Limk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Limk1
Entrez Gene ID	65172
Full Name	LIM domain kinase 1
General protein information	Preferred Names LIM domain kinase 1 Names LIM domain kinase 1 LIM motif-containing protein kinase 1 LIM-domain containing, protein kinase 1 LIMK-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q12
Summary	kinase that may be involved in contol of actin dynamics in the cell [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006249185.3	XP_006249247.1	LIM domain kinase 1 isoform X1
NM_031727.2	NP_113915.2	LIM domain kinase 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	LIMK1	NP_002305.1
Mus musculus (house mouse)	Limk1	NP_034847.1
Gallus gallus (chicken)	LIMK1	NP_989462.1
Xenopus tropicalis (tropical clawed frog)	limk1	NP_001072900.1
	LIMK1	XP_002803600.1
Rattus norvegicus (Norway rat)	Limk1	NP_113915.2
Pan troglodytes (chimpanzee)	LIMK1	XP_001148746.2
Canis lupus familiaris (dog)	LIMK1	XP_849646.1
Bos taurus (cattle)	LIMK1	NP_001193833.1
Drosophila melanogaster (fruit fly)	LIMK1	NP_511139.2
Danio rerio (zebrafish)	limk1a	NP_001036156.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011710	XP_320802.4

Gene Ontology
Bibliography

Related articles in PubMed

Electroacupuncture Regulates Hippocampal Synaptic Plasticity via miR-134-Mediated LIMK1 Function in Rats with Ischemic Stroke.
Liu W, Wu J, Huang J, Zhuo P, Lin Y, Wang L, Lin R, Chen L, Tao J
Neural plasticity20179545646(2017)

Sevoflurane induces cognitive impairments via the MiR-27b/LIMK1-signaling pathway in developing rats.
Yu Y, Zhang P, Yan J, Sun Y, Wu X, Xi S, Zhang L, Sun Y, Hu R, Jiang H
Inhalation toxicology28(14)731-738(2016 Dec)

Adaptor protein LRAP25 mediates myotonic dystrophy kinase-related Cdc42-binding kinase (MRCK) regulation of LIMK1 protein in lamellipodial F-actin dynamics.
Lee IC, Leung T, Tan I
The Journal of biological chemistry289(39)26989-7003(2014 Sep)

LIMK-dependent actin polymerization in primary sensory neurons promotes the development of inflammatory heat hyperalgesia in rats.
Li Y, Hu F, Chen HJ, Du YJ, Xie ZY, Zhang Y, Wang J, Wang Y
Science signaling7(331)ra61(2014 Jun)

CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and actin-dependent events.
Schultz ML, Tecedor L, Stein CS, Stamnes MA, Davidson BL
PloS one9(5)e96647(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MiR-134-mediated LIM domain kinase was involved in Regulates Hippocampal Synaptic Plasticity via miR-134-mediated LIMK1 function in rats with ischemic stroke induced hippocampal synaptic plasticity, which served as a contributor to improving learning and memory during the recovery stage of ischemic stroke.
Title: Electroacupuncture Regulates Hippocampal Synaptic Plasticity via miR-134-Mediated LIMK1 Function in Rats with Ischemic Stroke.

We demonstrated that LIMK1, as one target of miR-27b, might mediate sevoflurane-induced synapse deficits in the hippocampus. These results emphasize the potential role of LIMK1 in cognitive dysfunction in rats.
Title: Sevoflurane induces cognitive impairments via the MiR-27b/LIMK1-signaling pathway in developing rats.

the functional relevance of the LRAP25-MRCK complex in LIMK1-cofilin signaling and the importance of LRAP adaptors as key determinants of MRCK cellular localization and downstream specificities.
Title: Adaptor protein LRAP25 mediates myotonic dystrophy kinase-related Cdc42-binding kinase (MRCK) regulation of LIMK1 protein in lamellipodial F-actin dynamics.

Data indicate that LIM motif-containing protein kinases LIMK1 and LIMK2-dependent acin rearrangement is involved in the development of complete Freund's adjuvant (CFA)-induced inflammatory pain.
Title: LIMK-dependent actin polymerization in primary sensory neurons promotes the development of inflammatory heat hyperalgesia in rats.

the CaMKIIbeta phosphorylates LIMK1 at Thr-508 in the kinase domain and activates the cofilin-phosphorylating activity of LIMK1.
Title: CaMKII?-mediated LIM-kinase activation plays a crucial role in BDNF-induced neuritogenesis.

The following Limk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Limk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08603	NM_031727.2 Latest version!	Rattus norvegicus LIM domain kinase 1 (Limk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00
ORa34797	XM_006249185.3 Latest version!	Rattus norvegicus LIM domain kinase 1 (Limk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Limk1 ( NM_031727.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113915.2 Rattus norvegicus LIM domain kinase 1 (Limk1), mRNA.

Price & Availability↑

CloneID	ORa08603
Clone ID Related Accession (Same CDS sequence)	NM_031727.2
Accession Version	NM_031727.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Rattus norvegicus(Norway rat)
Product	LIM domain kinase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB726578.1, BF565872.1, CB702103.1, AW529884.1, CB691076.1, AC091000.2 and CV112377.1. On Oct 12, 2007 this sequence version replaced NM_031727.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D31873.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGTTGA CGCTACTTTG TTGCACCTGG AGGGAAGAAC GTATGGGAGA GGAAGGAAGC 
GAGTTGCCTG TGTGTGCGAG CTGTAGCCAG AGTATCTATG ACGGCCAGTA CCTCCAGGCC 
CTGAATGCTG ACTGGCATGC GGATTGCTTC AGGTGCTGCG AGTGTAGCAC CTCCCTTTCA 
CACCAGTACT ACGAGAAGGA TGGACAGCTC TTCTGCAAGA AGGACTACTG GGCCCGCTAT 
GGCGAGTCTT GCCACGGGTG CTCCGAACAC ATCACCAAAG GGCTGGTCAT GGTGGCCGGG 
GAGCTGAAGT ATCACCCCGA GTGCTTCATC TGCCTTGCCT GTGGAAACTT CATCGGTGAT 
GGGGACACCT ACACACTGGT GGAGCACTCC AAGCTGTACT GCGGGCAGTG CTACTACCAG 
ACTGTGGTAA CTCCAGTCAT CGAACAGATC CTCCCTGACT CACCCGGCTC CCACCTGCCC 
CACACAGTCA CCCTCGTGTC TATCCCAGCC TCTGCTCATG GCAAACGAGG CCTGTCTGTC 
TCCATCGACC CCCCTCACGG CCCACCAGGA TGTGGCACGG AGCATTCGCA CACGGTCCGA 
GTCCAAGGAG TGGACCCGGG CTGCATGAGC CCGGATGTGA AGAATTCCAT CCACATCGGA 
GACAGGATCT TGGAAATCAA CGGCACGCCC ATCCGGAATG TGCCGCTGGA CGAGATCGAC 
TTGCTGATCC AGGAGACCAG CCGCCTGCTC CAGCTGACCC TCGAGCACGA CCCCCATGAC 
TCACTGGGCC ATGGGCCAGT GTCGGATCCC AGCCCCCTGG CCTCCCCGGT ACACACTCCC 
AGCGGACAGG CAGGCAGCTC TGCCCGGCAG AAACCTGTCT TGAGGAGCTG CAGTATTGAC 
ACGTCCCCGG GTGCCGGCTC ACTAGTCTCA CCAGCCTCCC AGCGCAAGGA CCTGGGTCGC 
TCCGAATCCC TCCGAGTGGT TTGTCGACCA CACCGCATCT TCCGGCCGTC TGACCTCATC 
CATGGGGAGG TGTTGGGCAA GGGCTGTTTC GGCCAGGCCA TCAAGGTGAC ACATCGAGAA 
ACGGGCGAGG TGATGGTGAT GAAGGAACTC ATCCGGTTTG ACGAGGAGAC CCAGAGGACA 
TTCCTCAAGG AGGTGAAGGT CATGCGATGC CTGGAGCACC CTAACGTGCT CAAGTTCATC 
GGCGTGCTGT ACAAGGACAA GCGGCTGAAC TTCATCACCG AGTACATCAA GGGCGGCACC 
CTGCGGGGCA TCATCAAGAG CATGGACAGC CAGTACCCGT GGAGTCAGAG GGTCAGCTTT 
GCCAAGGACA TTGCATCAGG GATGGCCTAC CTCCATTCGA TGAACATCAT CCACCGAGAC 
CTCAACTCCC ACAACTGTCT GGTCCGTGAG AACAGGAACG TGGTGGTGGC TGACTTTGGG 
CTGGCTCGAC TCATGATCGA TGAGAAGGGT CAGTCTGAAG ACTTGCGTAG CCTTAAGAAG 
CCGGACCGCA AGAAACGGTA CACAGTGGTG GGTAACCCCT ACTGGATGGC GCCAGAGATG 
ATCAATGGTC GTAGTTACGA TGAAAAGGTG GACGTGTTTT CCTTTGGAAT TGTCCTTTGT 
GAGATTATCG GGCGTGTGAA TGCAGACCCT GACTATCTGC CCCGCACTAT GGACTTTGGC 
CTCAATGTAA GGGGTTTCCT GGACCGCTAC TGTCCACCAA ACTGTCCCCC AAGTTTCTTC 
CCCATCACTG TGCGCTGCTG TGACCTGGAT CCTGAGAAGA GGCCCTCTTT TGTAAAGCTG 
GAACAATGGC TAGAAACACT TCGCATGCAC TTGGCTGGTC ACCTGCCACT GGGCCCACAA 
CTGGAACAAC TGGAGCGGGG CTTCTGGGAG ACCTACCGGC GGGGCGAGAG CTCGCTACCC 
GCCCACCCCG AGGTCCCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113915.2
CDS	184..2127
Translation	MRLTLLCCTWREERMGEEGSELPVCASCSQSIYDGQYLQALNADWHADCFRCCECSTSLSHQYYEKDGQLFCKKDYWARYGESCHGCSEHITKGLVMVAGELKYHPECFICLACGNFIGDGDTYTLVEHSKLYCGQCYYQTVVTPVIEQILPDSPGSHLPHTVTLVSIPASAHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMSPDVKNSIHIGDRILEINGTPIRNVPLDEIDLLIQETSRLLQLTLEHDPHDSLGHGPVSDPSPLASPVHTPSGQAGSSARQKPVLRSCSIDTSPGAGSLVSPASQRKDLGRSESLRVVCRPHRIFRPSDLIHGEVLGKGCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENRNVVVADFGLARLMIDEKGQSEDLRSLKKPDRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEQWLETLRMHLAGHLPLGPQLEQLERGFWETYRRGESSLPAHPEVPD

Target ORF information:

RefSeq Version	NM_031727.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM domain kinase 1 (Limk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031727.2

ORF Insert Sequence:

ATGAGGTTGA CGCTACTTTG TTGCACCTGG AGGGAAGAAC GTATGGGAGA GGAAGGAAGC 
GAGTTGCCTG TGTGTGCGAG CTGTAGCCAG AGTATCTATG ACGGCCAGTA CCTCCAGGCC 
CTGAATGCTG ACTGGCATGC GGATTGCTTC AGGTGCTGCG AGTGTAGCAC CTCCCTTTCA 
CACCAGTACT ACGAGAAGGA TGGACAGCTC TTCTGCAAGA AGGACTACTG GGCCCGCTAT 
GGCGAGTCTT GCCACGGGTG CTCCGAACAC ATCACCAAAG GGCTGGTCAT GGTGGCCGGG 
GAGCTGAAGT ATCACCCCGA GTGCTTCATC TGCCTTGCCT GTGGAAACTT CATCGGTGAT 
GGGGACACCT ACACACTGGT GGAGCACTCC AAGCTGTACT GCGGGCAGTG CTACTACCAG 
ACTGTGGTAA CTCCAGTCAT CGAACAGATC CTCCCTGACT CACCCGGCTC CCACCTGCCC 
CACACAGTCA CCCTCGTGTC TATCCCAGCC TCTGCTCATG GCAAACGAGG CCTGTCTGTC 
TCCATCGACC CCCCTCACGG CCCACCAGGA TGTGGCACGG AGCATTCGCA CACGGTCCGA 
GTCCAAGGAG TGGACCCGGG CTGCATGAGC CCGGATGTGA AGAATTCCAT CCACATCGGA 
GACAGGATCT TGGAAATCAA CGGCACGCCC ATCCGGAATG TGCCGCTGGA CGAGATCGAC 
TTGCTGATCC AGGAGACCAG CCGCCTGCTC CAGCTGACCC TCGAGCACGA CCCCCATGAC 
TCACTGGGCC ATGGGCCAGT GTCGGATCCC AGCCCCCTGG CCTCCCCGGT ACACACTCCC 
AGCGGACAGG CAGGCAGCTC TGCCCGGCAG AAACCTGTCT TGAGGAGCTG CAGTATTGAC 
ACGTCCCCGG GTGCCGGCTC ACTAGTCTCA CCAGCCTCCC AGCGCAAGGA CCTGGGTCGC 
TCCGAATCCC TCCGAGTGGT TTGTCGACCA CACCGCATCT TCCGGCCGTC TGACCTCATC 
CATGGGGAGG TGTTGGGCAA GGGCTGTTTC GGCCAGGCCA TCAAGGTGAC ACATCGAGAA 
ACGGGCGAGG TGATGGTGAT GAAGGAACTC ATCCGGTTTG ACGAGGAGAC CCAGAGGACA 
TTCCTCAAGG AGGTGAAGGT CATGCGATGC CTGGAGCACC CTAACGTGCT CAAGTTCATC 
GGCGTGCTGT ACAAGGACAA GCGGCTGAAC TTCATCACCG AGTACATCAA GGGCGGCACC 
CTGCGGGGCA TCATCAAGAG CATGGACAGC CAGTACCCGT GGAGTCAGAG GGTCAGCTTT 
GCCAAGGACA TTGCATCAGG GATGGCCTAC CTCCATTCGA TGAACATCAT CCACCGAGAC 
CTCAACTCCC ACAACTGTCT GGTCCGTGAG AACAGGAACG TGGTGGTGGC TGACTTTGGG 
CTGGCTCGAC TCATGATCGA TGAGAAGGGT CAGTCTGAAG ACTTGCGTAG CCTTAAGAAG 
CCGGACCGCA AGAAACGGTA CACAGTGGTG GGTAACCCCT ACTGGATGGC GCCAGAGATG 
ATCAATGGTC GTAGTTACGA TGAAAAGGTG GACGTGTTTT CCTTTGGAAT TGTCCTTTGT 
GAGATTATCG GGCGTGTGAA TGCAGACCCT GACTATCTGC CCCGCACTAT GGACTTTGGC 
CTCAATGTAA GGGGTTTCCT GGACCGCTAC TGTCCACCAA ACTGTCCCCC AAGTTTCTTC 
CCCATCACTG TGCGCTGCTG TGACCTGGAT CCTGAGAAGA GGCCCTCTTT TGTAAAGCTG 
GAACAATGGC TAGAAACACT TCGCATGCAC TTGGCTGGTC ACCTGCCACT GGGCCCACAA 
CTGGAACAAC TGGAGCGGGG CTTCTGGGAG ACCTACCGGC GGGGCGAGAG CTCGCTACCC 
GCCCACCCCG AGGTCCCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Limk1 ( XM_006249185.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249247.1 Rattus norvegicus LIM domain kinase 1 (Limk1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa34797
Clone ID Related Accession (Same CDS sequence)	XM_006249185.3
Accession Version	XM_006249185.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LIM domain kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249185.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCAG CTTTGGCCCT GAGAAGGACC CGCTGCCTCC GTGGGGCTAA GTGCTGCGAG 
TGTAGCACCT CCCTTTCACA CCAGTACTAC GAGAAGGATG GACAGCTCTT CTGCAAGAAG 
GACTACTGGG CCCGCTATGG CGAGTCTTGC CACGGGTGCT CCGAACACAT CACCAAAGGG 
CTGGTCATGG TGGCCGGGGA GCTGAAGTAT CACCCCGAGT GCTTCATCTG CCTTGCCTGT 
GGAAACTTCA TCGGTGATGG GGACACCTAC ACACTGGTGG AGCACTCCAA GCTGTACTGC 
GGGCAGTGCT ACTACCAGAC TGTGGTAACT CCAGTCATCG AACAGATCCT CCCTGACTCA 
CCCGGCTCCC ACCTGCCCCA CACAGTCACC CTCGTGTCTA TCCCAGCCTC TGCTCATGGC 
AAACGAGGCC TGTCTGTCTC CATCGACCCC CCTCACGGCC CACCAGGATG TGGCACGGAG 
CATTCGCACA CGGTCCGAGT CCAAGGAGTG GACCCGGGCT GCATGAGCCC GGATGTGAAG 
AATTCCATCC ACATCGGAGA CAGGATCTTG GAAATCAACG GCACGCCCAT CCGGAATGTG 
CCGCTGGACG AGATCGACTT GCTGATCCAG GAGACCAGCC GCCTGCTCCA GCTGACCCTC 
GAGCACGACC CCCATGACTC ACTGGGCCAT GGGCCAGTGT CGGATCCCAG CCCCCTGGCC 
TCCCCGGTAC ACACTCCCAG CGGACAGGCA GGCAGCTCTG CCCGGCAGAA ACCTGTCTTG 
AGGAGCTGCA GTATTGACAC GTCCCCGGGT GCCGGCTCAC TAGTCTCACC AGCCTCCCAG 
CGCAAGGACC TGGGTCGCTC CGAATCCCTC CGAGTGGTTT GTCGACCACA CCGCATCTTC 
CGGCCGTCTG ACCTCATCCA TGGGGAGGTG TTGGGCAAGG GCTGTTTCGG CCAGGCCATC 
AAGGTGACAC ATCGAGAAAC GGGCGAGGTG ATGGTGATGA AGGAACTCAT CCGGTTTGAC 
GAGGAGACCC AGAGGACATT CCTCAAGGAG GTGAAGGTCA TGCGATGCCT GGAGCACCCT 
AACGTGCTCA AGTTCATCGG CGTGCTGTAC AAGGACAAGC GGCTGAACTT CATCACCGAG 
TACATCAAGG GCGGCACCCT GCGGGGCATC ATCAAGAGCA TGGACAGCCA GTACCCGTGG 
AGTCAGAGGG TCAGCTTTGC CAAGGACATT GCATCAGGGA TGGCCTACCT CCATTCGATG 
AACATCATCC ACCGAGACCT CAACTCCCAC AACTGTCTGG TCCGTGAGAA CAGGAACGTG 
GTGGTGGCTG ACTTTGGGCT GGCTCGACTC ATGATCGATG AGAAGGGTCA GTCTGAAGAC 
TTGCGTAGCC TTAAGAAGCC GGACCGCAAG AAACGGTACA CAGTGGTGGG TAACCCCTAC 
TGGATGGCGC CAGAGATGAT CAATGGTCGT AGTTACGATG AAAAGGTGGA CGTGTTTTCC 
TTTGGAATTG TCCTTTGTGA GATTATCGGG CGTGTGAATG CAGACCCTGA CTATCTGCCC 
CGCACTATGG ACTTTGGCCT CAATGTAAGG GGTTTCCTGG ACCGCTACTG TCCACCAAAC 
TGTCCCCCAA GTTTCTTCCC CATCACTGTG CGCTGCTGTG ACCTGGATCC TGAGAAGAGG 
CCCTCTTTTG TAAAGCTGGA ACAATGGCTA GAAACACTTC GCATGCACTT GGCTGGTCAC 
CTGCCACTGG GCCCACAACT GGAACAACTG GAGCGGGGCT TCTGGGAGAC CTACCGGCGG 
GGCGAGAGCT CGCTACCCGC CCACCCCGAG GTCCCTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249247.1
CDS	191..2032
Translation	MQPALALRRTRCLRGAKCCECSTSLSHQYYEKDGQLFCKKDYWARYGESCHGCSEHITKGLVMVAGELKYHPECFICLACGNFIGDGDTYTLVEHSKLYCGQCYYQTVVTPVIEQILPDSPGSHLPHTVTLVSIPASAHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMSPDVKNSIHIGDRILEINGTPIRNVPLDEIDLLIQETSRLLQLTLEHDPHDSLGHGPVSDPSPLASPVHTPSGQAGSSARQKPVLRSCSIDTSPGAGSLVSPASQRKDLGRSESLRVVCRPHRIFRPSDLIHGEVLGKGCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENRNVVVADFGLARLMIDEKGQSEDLRSLKKPDRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEQWLETLRMHLAGHLPLGPQLEQLERGFWETYRRGESSLPAHPEVPD

Target ORF information:

RefSeq Version	XM_006249185.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM domain kinase 1 (Limk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249185.3

ORF Insert Sequence:

ATGCAGCCAG CTTTGGCCCT GAGAAGGACC CGCTGCCTCC GTGGGGCTAA GTGCTGCGAG 
TGTAGCACCT CCCTTTCACA CCAGTACTAC GAGAAGGATG GACAGCTCTT CTGCAAGAAG 
GACTACTGGG CCCGCTATGG CGAGTCTTGC CACGGGTGCT CCGAACACAT CACCAAAGGG 
CTGGTCATGG TGGCCGGGGA GCTGAAGTAT CACCCCGAGT GCTTCATCTG CCTTGCCTGT 
GGAAACTTCA TCGGTGATGG GGACACCTAC ACACTGGTGG AGCACTCCAA GCTGTACTGC 
GGGCAGTGCT ACTACCAGAC TGTGGTAACT CCAGTCATCG AACAGATCCT CCCTGACTCA 
CCCGGCTCCC ACCTGCCCCA CACAGTCACC CTCGTGTCTA TCCCAGCCTC TGCTCATGGC 
AAACGAGGCC TGTCTGTCTC CATCGACCCC CCTCACGGCC CACCAGGATG TGGCACGGAG 
CATTCGCACA CGGTCCGAGT CCAAGGAGTG GACCCGGGCT GCATGAGCCC GGATGTGAAG 
AATTCCATCC ACATCGGAGA CAGGATCTTG GAAATCAACG GCACGCCCAT CCGGAATGTG 
CCGCTGGACG AGATCGACTT GCTGATCCAG GAGACCAGCC GCCTGCTCCA GCTGACCCTC 
GAGCACGACC CCCATGACTC ACTGGGCCAT GGGCCAGTGT CGGATCCCAG CCCCCTGGCC 
TCCCCGGTAC ACACTCCCAG CGGACAGGCA GGCAGCTCTG CCCGGCAGAA ACCTGTCTTG 
AGGAGCTGCA GTATTGACAC GTCCCCGGGT GCCGGCTCAC TAGTCTCACC AGCCTCCCAG 
CGCAAGGACC TGGGTCGCTC CGAATCCCTC CGAGTGGTTT GTCGACCACA CCGCATCTTC 
CGGCCGTCTG ACCTCATCCA TGGGGAGGTG TTGGGCAAGG GCTGTTTCGG CCAGGCCATC 
AAGGTGACAC ATCGAGAAAC GGGCGAGGTG ATGGTGATGA AGGAACTCAT CCGGTTTGAC 
GAGGAGACCC AGAGGACATT CCTCAAGGAG GTGAAGGTCA TGCGATGCCT GGAGCACCCT 
AACGTGCTCA AGTTCATCGG CGTGCTGTAC AAGGACAAGC GGCTGAACTT CATCACCGAG 
TACATCAAGG GCGGCACCCT GCGGGGCATC ATCAAGAGCA TGGACAGCCA GTACCCGTGG 
AGTCAGAGGG TCAGCTTTGC CAAGGACATT GCATCAGGGA TGGCCTACCT CCATTCGATG 
AACATCATCC ACCGAGACCT CAACTCCCAC AACTGTCTGG TCCGTGAGAA CAGGAACGTG 
GTGGTGGCTG ACTTTGGGCT GGCTCGACTC ATGATCGATG AGAAGGGTCA GTCTGAAGAC 
TTGCGTAGCC TTAAGAAGCC GGACCGCAAG AAACGGTACA CAGTGGTGGG TAACCCCTAC 
TGGATGGCGC CAGAGATGAT CAATGGTCGT AGTTACGATG AAAAGGTGGA CGTGTTTTCC 
TTTGGAATTG TCCTTTGTGA GATTATCGGG CGTGTGAATG CAGACCCTGA CTATCTGCCC 
CGCACTATGG ACTTTGGCCT CAATGTAAGG GGTTTCCTGG ACCGCTACTG TCCACCAAAC 
TGTCCCCCAA GTTTCTTCCC CATCACTGTG CGCTGCTGTG ACCTGGATCC TGAGAAGAGG 
CCCTCTTTTG TAAAGCTGGA ACAATGGCTA GAAACACTTC GCATGCACTT GGCTGGTCAC 
CTGCCACTGG GCCCACAACT GGAACAACTG GAGCGGGGCT TCTGGGAGAC CTACCGGCGG 
GGCGAGAGCT CGCTACCCGC CCACCCCGAG GTCCCTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Electroacupuncture Regulates Hippocampal Synaptic Plasticity via miR-134-Mediated LIMK1 Function in Rats with Ischemic Stroke.
Neural plasticity20179545646(2017)
Liu W,Wu J,Huang J,Zhuo P,Lin Y,Wang L,Lin R,Chen L,Tao J

Sevoflurane induces cognitive impairments via the MiR-27b/LIMK1-signaling pathway in developing rats.
Inhalation toxicology28(14)731-738(2016 Dec)
Yu Y,Zhang P,Yan J,Sun Y,Wu X,Xi S,Zhang L,Sun Y,Hu R,Jiang H

Adaptor protein LRAP25 mediates myotonic dystrophy kinase-related Cdc42-binding kinase (MRCK) regulation of LIMK1 protein in lamellipodial F-actin dynamics.
The Journal of biological chemistry289(39)26989-7003(2014 Sep)
Lee IC,Leung T,Tan I

LIMK-dependent actin polymerization in primary sensory neurons promotes the development of inflammatory heat hyperalgesia in rats.
Science signaling7(331)ra61(2014 Jun)
Li Y,Hu F,Chen HJ,Du YJ,Xie ZY,Zhang Y,Wang J,Wang Y

CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and actin-dependent events.
PloS one9(5)e96647(2014)
Schultz ML,Tecedor L,Stein CS,Stamnes MA,Davidson BL

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Limk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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