gRNAs
The following limk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the limk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OXa20848 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001079432.1 | |
| Accession Version | NM_001079432.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-22 | |
| Organism | Xenopus tropicalis(tropical clawed frog) | |
| Product | LIM domain kinase 1 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC121406.1. ##Evidence-Data-START## Transcript exon combination :: BC121406.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN06679859 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGAGGTTGA TGTTACTGTG CTGCTCCTGG AGTGAGGAAC ACATGGGAGA GGAGGAAGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001072900.1 |
| CDS | 327..2174 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001079432.1 |
1 | ATGAGGTTGA TGTTACTGTG CTGCTCCTGG AGTGAGGAAC ACATGGGAGA GGAGGAAGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OXa29733 | |
| Clone ID Related Accession (Same CDS sequence) | XM_012957333.2 , XM_012957333.3 | |
| Accession Version | XM_012957333.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-09-07 | |
| Organism | Xenopus tropicalis(tropical clawed frog) | |
| Product | LIM domain kinase 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957333.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGGAGA AGCAGCAGGC TGGGAAGAGA CAATGTAGTG ACTGCGGTGT GTCCTTGTCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_012812787.1 |
| CDS | 68..1792 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_012957333.2 |
1 | ATGGAGGAGA AGCAGCAGGC TGGGAAGAGA CAATGTAGTG ACTGCGGTGT GTCCTTGTCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OXa29733 | |
| Clone ID Related Accession (Same CDS sequence) | XM_012957333.2 , XM_012957333.3 | |
| Accession Version | XM_012957333.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-17 | |
| Organism | Xenopus tropicalis(tropical clawed frog) | |
| Product | LIM domain kinase 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957333.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGGAGA AGCAGCAGGC TGGGAAGAGA CAATGTAGTG ACTGCGGTGT GTCCTTGTCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_012812787.1 |
| CDS | 70..1794 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_012957333.3 |
1 | ATGGAGGAGA AGCAGCAGGC TGGGAAGAGA CAATGTAGTG ACTGCGGTGT GTCCTTGTCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
BMP gradients steer nerve growth cones by a balancing act of LIM kinase and Slingshot phosphatase on ADF/cofilin. |
Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative. |
