TMEM189-UBE2V1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMEM189-UBE2V1
Entrez Gene ID	387522
Full Name	TMEM189-UBE2V1 readthrough
Synonyms	CROC-1B,CROC1B,KUA-UEV
General protein information	Preferred Names TMEM189-UBE2V1 readthrough Names TMEM189-UBE2V1 fusion protein TMEM189-UBE2V1 readthrough transcript transmembrane protein 189-ubiquitin-conjugating enzyme E2 variant 1 read-through
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q13.13
Summary	The TMEM189-UEV mRNA is an infrequent but naturally occurring read-through transcript of the neighboring TMEM189 and UBE2V1 genes. Ubiquitin-conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein produced by this transcript has UEV1 B domains but the protein is localized to the cytoplasm rather than to the nucleus. The significance of this read-through mRNA and the function of its protein product has not yet been determined. [provided by RefSeq, Oct 2010].

mRNA and Protein(s)

mRNA	Protein	Name
NM_199203.2	NP_954673.1	TMEM189-UBE2V1 fusion protein

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os03g0712300	NP_001051063.1
Homo sapiens (human)	TMEM189-UBE2V1	NP_954673.1
Arabidopsis thaliana (thale cress)	MMZ1	NP_564191.1
Arabidopsis thaliana (thale cress)	MMZ2	NP_564994.1

Gene Ontology
Bibliography

Related articles in PubMed

An approach based on a genome-wide association study reveals candidate loci for narcolepsy.
Shimada M, Miyagawa T, Kawashima M, Tanaka S, Honda Y, Honda M, Tokunaga K
Human genetics128(4)433-41(2010 Oct)

Expression of conjoined genes: another mechanism for gene regulation in eukaryotes.
Prakash T, Sharma VK, Adati N, Ozawa R, Kumar N, Nishida Y, Fujikake T, Takeda T, Taylor TD
PloS one5(10)e13284(2010 Oct)

Fusion of the human gene for the polyubiquitination coeffector UEV1 with Kua, a newly identified gene.
Thomson TM, Lozano JJ, Loukili N, Carri? R, Serras F, Cormand B, Valeri M, D?az VM, Abril J, Burset M, Merino J, Macaya A, Corominas M, Guig? R
Genome research10(11)1743-56(2000 Nov)

A new function evolved from gene fusion.
Long M
Genome research10(11)1655-7(2000 Nov)

Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain.
Deng L, Wang C, Spencer E, Yang L, Braun A, You J, Slaughter C, Pickart C, Chen ZJ
Cell103(2)351-61(2000 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: An approach based on a genome-wide association study reveals candidate loci for narcolepsy.

The following TMEM189-UBE2V1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM189-UBE2V1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu08821	NM_199203.2 Latest version!	Homo sapiens TMEM189-UBE2V1 readthrough (TMEM189-UBE2V1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEM189-UBE2V1 ( NM_199203.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_954673.1 Homo sapiens TMEM189-UBE2V1 readthrough (TMEM189-UBE2V1), mRNA.

Price & Availability↑

CloneID	OHu08821
Clone ID Related Accession (Same CDS sequence)	NM_199203.2
Accession Version	NM_199203.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-30
Organism	Homo sapiens(human)
Product	TMEM189-UBE2V1 fusion protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL034423.21, AK023028.1, U39361.1 and CR738799.1. On Jun 25, 2009 this sequence version replaced NM_199203.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments and on experimental data in PMID:11076860. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## readthrough transcript :: includes exons from GeneID 7335, 387521 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGCG CCGAGGACTG GCCGGGCCAG CAGCTGGAGC TGGACGAGGA CGAGGCGTCT 
TGTTGCCGCT GGGGCGCGCA GCACGCCGGG GCCCGCGAGC TGGCTGCGCT CTACTCGCCA 
GGCAAGCGCC TCCAGGAGTG GTGCTCTGTG ATCCTGTGCT TCAGCCTCAT CGCCCACAAC 
CTGGTCCATC TCCTGCTGCT GGCCCGCTGG GAGGACACAC CCCTCGTCAT ACTCGGTGTT 
GTTGCAGGGG CTCTCATTGC TGACTTCTTG TCTGGCCTGG TACACTGGGG TGCTGACACA 
TGGGGCTCTG TGGAGCTGCC CATTGTGGGG AAGGCTTTCA TCCGACCCTT CCGGGAGCAC 
CACATTGACC CGACAGCTAT CACACGGCAC GACTTCATCG AGACCAACGG GGACAACTGC 
CTGGTGACAC TGCTGCCGCT GCTAAACATG GCCTACAAGT TCCGCACCCA CAGCCCTGAA 
GCCCTGGAGC AGCTATACCC CTGGGAGTGC TTCGTCTTCT GCCTGATCAT CTTCGGCACC 
TTCACCAACC AGATCCACAA GTGGTCGCAC ACGTACTTTG GGCTGCCACG CTGGGTCACC 
CTCCTGCAGG ACTGGCATGT CATCCTGCCA CGTAAACACC ATCGCATCCA CCACGTCTCA 
CCCCACGAGA CCTACTTCTG CATCACCACA GGAGTAAAAG TCCCTCGCAA TTTCCGACTG 
TTGGAAGAAC TCGAAGAAGG CCAGAAAGGA GTAGGAGATG GCACAGTTAG CTGGGGTCTA 
GAAGATGACG AAGACATGAC ACTTACAAGA TGGACAGGGA TGATAATTGG GCCTCCAAGA 
ACAATTTATG AAAACCGAAT ATACAGCCTT AAAATAGAAT GTGGACCTAA ATACCCAGAA 
GCACCCCCCT TTGTAAGATT TGTAACAAAA ATTAATATGA ATGGAGTAAA TAGTTCTAAT 
GGAGTGGTGG ACCCAAGAGC CATATCAGTG CTAGCAAAAT GGCAGAATTC ATATAGCATC 
AAAGTTGTCC TGCAAGAGCT TCGGCGCCTA ATGATGTCTA AAGAAAATAT GAAACTCCCT 
CAGCCGCCCG AAGGACAGTG TTACAGCAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954673.1
CDS	162..1274
Translation	MAGAEDWPGQQLELDEDEASCCRWGAQHAGARELAALYSPGKRLQEWCSVILCFSLIAHNLVHLLLLARWEDTPLVILGVVAGALIADFLSGLVHWGADTWGSVELPIVGKAFIRPFREHHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTHSPEALEQLYPWECFVFCLIIFGTFTNQIHKWSHTYFGLPRWVTLLQDWHVILPRKHHRIHHVSPHETYFCITTGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQCYSN

Target ORF information:

RefSeq Version	NM_199203.2
Organism	Homo sapiens(human)
Definition	Homo sapiens TMEM189-UBE2V1 readthrough (TMEM189-UBE2V1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199203.2

ORF Insert Sequence:

ATGGCGGGCG CCGAGGACTG GCCGGGCCAG CAGCTGGAGC TGGACGAGGA CGAGGCGTCT 
TGTTGCCGCT GGGGCGCGCA GCACGCCGGG GCCCGCGAGC TGGCTGCGCT CTACTCGCCA 
GGCAAGCGCC TCCAGGAGTG GTGCTCTGTG ATCCTGTGCT TCAGCCTCAT CGCCCACAAC 
CTGGTCCATC TCCTGCTGCT GGCCCGCTGG GAGGACACAC CCCTCGTCAT ACTCGGTGTT 
GTTGCAGGGG CTCTCATTGC TGACTTCTTG TCTGGCCTGG TACACTGGGG TGCTGACACA 
TGGGGCTCTG TGGAGCTGCC CATTGTGGGG AAGGCTTTCA TCCGACCCTT CCGGGAGCAC 
CACATTGACC CGACAGCTAT CACACGGCAC GACTTCATCG AGACCAACGG GGACAACTGC 
CTGGTGACAC TGCTGCCGCT GCTAAACATG GCCTACAAGT TCCGCACCCA CAGCCCTGAA 
GCCCTGGAGC AGCTATACCC CTGGGAGTGC TTCGTCTTCT GCCTGATCAT CTTCGGCACC 
TTCACCAACC AGATCCACAA GTGGTCGCAC ACGTACTTTG GGCTGCCACG CTGGGTCACC 
CTCCTGCAGG ACTGGCATGT CATCCTGCCA CGTAAACACC ATCGCATCCA CCACGTCTCA 
CCCCACGAGA CCTACTTCTG CATCACCACA GGAGTAAAAG TCCCTCGCAA TTTCCGACTG 
TTGGAAGAAC TCGAAGAAGG CCAGAAAGGA GTAGGAGATG GCACAGTTAG CTGGGGTCTA 
GAAGATGACG AAGACATGAC ACTTACAAGA TGGACAGGGA TGATAATTGG GCCTCCAAGA 
ACAATTTATG AAAACCGAAT ATACAGCCTT AAAATAGAAT GTGGACCTAA ATACCCAGAA 
GCACCCCCCT TTGTAAGATT TGTAACAAAA ATTAATATGA ATGGAGTAAA TAGTTCTAAT 
GGAGTGGTGG ACCCAAGAGC CATATCAGTG CTAGCAAAAT GGCAGAATTC ATATAGCATC 
AAAGTTGTCC TGCAAGAGCT TCGGCGCCTA ATGATGTCTA AAGAAAATAT GAAACTCCCT 
CAGCCGCCCG AAGGACAGTG TTACAGCAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An approach based on a genome-wide association study reveals candidate loci for narcolepsy.
Human genetics128(4)433-41(2010 Oct)
Shimada M,Miyagawa T,Kawashima M,Tanaka S,Honda Y,Honda M,Tokunaga K

Expression of conjoined genes: another mechanism for gene regulation in eukaryotes.
PloS one5(10)e13284(2010 Oct)
Prakash T,Sharma VK,Adati N,Ozawa R,Kumar N,Nishida Y,Fujikake T,Takeda T,Taylor TD

Fusion of the human gene for the polyubiquitination coeffector UEV1 with Kua, a newly identified gene.
Genome research10(11)1743-56(2000 Nov)
Thomson TM,Lozano JJ,Loukili N,Carri? R,Serras F,Cormand B,Valeri M,D?az VM,Abril J,Burset M,Merino J,Macaya A,Corominas M,Guig? R

A new function evolved from gene fusion.
Genome research10(11)1655-7(2000 Nov)
Long M

Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain.
Cell103(2)351-61(2000 Oct)
Deng L,Wang C,Spencer E,Yang L,Braun A,You J,Slaughter C,Pickart C,Chen ZJ

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TMEM189-UBE2V1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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