GUCY1A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GUCY1A1
Entrez Gene ID	2982
Full Name	guanylate cyclase 1 soluble subunit alpha 1
Synonyms	GC-SA3,GUC1A3,GUCA3,GUCSA3,GUCY1A3,MYMY6
General protein information	Preferred Names guanylate cyclase 1 soluble subunit alpha 1 Names guanylate cyclase soluble subunit alpha-3 GC-S-alpha-1 GCS-alpha-3 guanylate cyclase 1, soluble, alpha 3 soluble guanylate cyclase large subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q32.1
Summary	Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012].
Disorder MIM:	139396
Disorder Html:	Moyamoya 6 with achalasia, 615750 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006714198.2	XP_006714261.1	guanylate cyclase soluble subunit alpha-3 isoform X2
XM_006714197.1	XP_006714260.1	guanylate cyclase soluble subunit alpha-3 isoform X1
NM_000856.5	NP_000847.2	guanylate cyclase soluble subunit alpha-1 isoform A
NM_001130684.2	NP_001124156.1	guanylate cyclase soluble subunit alpha-1 isoform A
NM_001130684.3	NP_001124156.1	guanylate cyclase soluble subunit alpha-1 isoform A
NM_001256449.2	NP_001243378.1	guanylate cyclase soluble subunit alpha-1 isoform A
XM_006714197.2	XP_006714260.1	guanylate cyclase soluble subunit alpha-1 isoform X1
NM_001130682.2	NP_001124154.1	guanylate cyclase soluble subunit alpha-1 isoform A
NM_001130687.2	NP_001124159.1	guanylate cyclase soluble subunit alpha-1 isoform D
XM_005262955.3	XP_005263012.1	guanylate cyclase soluble subunit alpha-1 isoform X1
XM_005262955.2	XP_005263012.1	guanylate cyclase soluble subunit alpha-3 isoform X1
XM_006714196.1	XP_006714259.1	guanylate cyclase soluble subunit alpha-3 isoform X1
NM_001130683.4	NP_001124155.1	guanylate cyclase soluble subunit alpha-1 isoform A
XM_006714196.2	XP_006714259.1	guanylate cyclase soluble subunit alpha-1 isoform X1
XM_011531899.1	XP_011530201.1	guanylate cyclase soluble subunit alpha-3 isoform X1
XM_005262955.1	XP_005263012.1	guanylate cyclase soluble subunit alpha-3 isoform X1
XM_005262956.2	XP_005263013.1	guanylate cyclase soluble subunit alpha-3 isoform X2
NM_001130685.2	NP_001124157.1	guanylate cyclase soluble subunit alpha-1 isoform B
NM_001130683.3	NP_001124155.1	guanylate cyclase soluble subunit alpha-1 isoform A
XM_005262956.3	XP_005263013.1	guanylate cyclase soluble subunit alpha-1 isoform X2
XM_011531900.2	XP_011530202.1	guanylate cyclase soluble subunit alpha-1 isoform X2
NM_001256449.1	NP_001243378.1	guanylate cyclase soluble subunit alpha-1 isoform A
XM_005262957.2	XP_005263014.1	guanylate cyclase soluble subunit alpha-3 isoform X2
NM_000856.6	NP_000847.2	guanylate cyclase soluble subunit alpha-1 isoform A
NM_001130682.3	NP_001124154.1	guanylate cyclase soluble subunit alpha-1 isoform A
NM_001130685.3	NP_001124157.1	guanylate cyclase soluble subunit alpha-1 isoform B
XM_005262957.3	XP_005263014.1	guanylate cyclase soluble subunit alpha-1 isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	gucy1a3	XP_004911212.1
Canis lupus familiaris (dog)	GUCY1A3	NP_001018045.1
Bos taurus (cattle)	GUCY1A3	NP_786972.1
Homo sapiens (human)	GUCY1A3	NP_001124154.1
Mus musculus (house mouse)	Gucy1a3	NP_068696.2
Rattus norvegicus (Norway rat)	Gucy1a3	NP_058786.2
Pan troglodytes (chimpanzee)	GUCY1A3	XP_003310599.1
	GUCY1A3	XP_002804280.1
Gallus gallus (chicken)	GUCY1A3	XP_420375.1
Danio rerio (zebrafish)	gucy1a3	NP_001017725.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of a soluble guanylate cyclase in RBCs: preserved activity in patients with coronary artery disease.
Cortese-Krott MM, Mergia E, Kramer CM, L?ckst?dt W, Yang J, Wolff G, Panknin C, Bracht T, Sitek B, Pernow J, Stasch JP, Feelisch M, Koesling D, Kelm M
Redox biology14328-337(2018 Apr)

Phenotypic Consequences of a Genetic Predisposition to Enhanced Nitric Oxide Signaling.
Emdin CA, Khera AV, Klarin D, Natarajan P, Zekavat SM, Nomura A, Haas M, Aragam K, Ardissino D, Wilson JG, Schunkert H, McPherson R, Watkins H, Elosua R, Bown MJ, Samani NJ, Baber U, Erdmann J, Gormley P, Palotie A, Stitziel NO, Gupta N, Danesh J, Saleheen D, Gabriel S, Kathiresan S
Circulation137(3)222-232(2018 Jan)

Functional Characterization of the
Kessler T, Wobst J, Wolf B, Eckhold J, Vilne B, Hollstein R, von Ameln S, Dang TA, Sager HB, Moritz Rumpf P, Aherrahrou R, Kastrati A, Bj?rkegren JLM, Erdmann J, Lusis AJ, Civelek M, Kaiser FJ, Schunkert H
Circulation136(5)476-489(2017 Aug)

Peptide B targets soluble guanylyl cyclase ?1 and kills prostate cancer cells.
Zhou J, Gao S, Hsieh CL, Malla M, Shemshedini L
PloS one12(8)e0184088(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rs7692387 is located in an intronic site that modulates GUCY1A3 promoter activity. The transcription factor ZEB1 binds preferentially to the nonrisk allele, leading to an increase in GUCY1A3 expression, higher sGC levels, and higher sGC activity after stimulation.
Title: Functional Characterization of the

Peptide B-8R killed both androgen-dependent and androgen-independent prostate cancer cells that expressed sGCalpha1, but not cells that do not express this gene. Peptide B-8R induced apoptosis of prostate cancer cells.
Title: Peptide B targets soluble guanylyl cyclase ?1 and kills prostate cancer cells.

Mutations in the GUCY1A3 gene are associated with moyamoya disease, achalasia, and hypertension.
Title: Disrupted nitric oxide signaling due to GUCY1A3 mutations increases risk for moyamoya disease, achalasia and hypertension.

In conclusion, rare coding variants in GUCY1A3 lead to reduced cGMP formation which can be rescued by a soluble guanylyl cyclase stimulator in vitro
Title: Stimulators of the soluble guanylyl cyclase: promising functional insights from rare coding atherosclerosis-related GUCY1A3 variants.

Expression of the alpha1-A680T sGC variant in reporter cells resulted in higher cyclic guanosine monophosphate production compared with the wild-type enzyme and the purified alpha1-A680T sGC exhibited enhanced sensitivity to nitric oxide in vitro.
Title: ?1-A680T variant in GUCY1A3 as a candidate conferring protection from pulmonary hypertension among Kyrgyz highlanders.

The following GUCY1A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUCY1A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25093	NM_001130687.2 Latest version!	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu25145	NM_001256449.2 Latest version!	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25145	NM_001130683.4 Latest version!	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25145	NM_001130682.3 Latest version!	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25145	NM_000856.6 Latest version!	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25145	NM_001130684.3 Latest version!	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25145	XM_006714196.2 Latest version!	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25145	XM_011531899.1 Latest version!	Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25145	XM_006714197.2 Latest version!	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25145	XM_005262955.3 Latest version!	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25175	NM_001130685.3 Latest version!	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu25175	XM_011531900.2 Latest version!	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu25175	XM_006714198.2 Latest version!	Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu25175	XM_005262957.3 Latest version!	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu25175	XM_005262956.3 Latest version!	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
View Old Accession Versions >>
OHu25145	NM_001130684.2	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25145	XM_006714197.1	Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25145	XM_005262955.2	Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25145	XM_006714196.1	Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25145	XM_005262955.1	Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25145	NM_001130683.3	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25175	XM_005262956.2	Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu25145	NM_001256449.1	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25145	NM_000856.5	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25145	NM_001130682.2	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25175	NM_001130685.2	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu25175	XM_005262957.2	Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GUCY1A1 ( NM_001130687.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124159.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 7, mRNA.

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CloneID	OHu25093
Clone ID Related Accession (Same CDS sequence)	NM_001130687.2
Accession Version	NM_001130687.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA793192.1, AK309950.1 and AC104083.3. On Jan 28, 2012 this sequence version replaced NM_001130687.1. Transcript Variant: This variant (7) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (D) is shorter at the C-terminus compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK309950.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124159.1
CDS	443..2317
Translation	MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYR

Target ORF information:

RefSeq Version	NM_001130687.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130687.2

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( NM_000856.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000847.2 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 1, mRNA.

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CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	NM_000856.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA793192.1, Y15723.1, AC104083.3 and CD671731.1. This sequence is a reference standard in the RefSeqGene project. On Jan 28, 2012 this sequence version replaced NM_000856.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (A). Variants 1, 2, 3, 4, and 8 all encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.175812.1, Y15723.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000847.2
CDS	537..2609
Translation	MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	NM_000856.5
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000856.5

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( NM_001130684.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124156.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 4, mRNA.

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CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	NM_001130684.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104083.3, Y15723.1 and CD671731.1. On Jan 28, 2012 this sequence version replaced NM_001130684.1. Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1, and encodes isoform A. Variants 1, 2, 3, 4, and 8 all encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028384.2, SRR1803617.141532.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124156.1
CDS	383..2455
Translation	MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	NM_001130684.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130684.2

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( NM_001130684.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124156.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	NM_001130684.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104083.3, Y15723.1 and CD671731.1. On Jun 2, 2019 this sequence version replaced NM_001130684.2. Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1, and encodes isoform A. Variants 1, 2, 3, 4, and 8 all encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.141532.1, SRR1803612.106684.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124156.1
CDS	384..2456
Translation	MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	NM_001130684.3
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130684.3

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( NM_001256449.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243378.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 8, mRNA.

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CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	NM_001256449.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104083.3, AK096588.1, Y15723.1 and CD671731.1. On Jun 2, 2019 this sequence version replaced NM_001256449.1. Transcript Variant: This variant (8) differs in the 5' UTR, compared to variant 1, and encodes isoform A. Variants 1, 2, 3, 4, and 8 all encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK096588.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243378.1
CDS	489..2561
Translation	MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	NM_001256449.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256449.2

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( NM_001130682.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124154.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	NM_001130682.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA793192.1, Y15723.1, AC104083.3 and CD671731.1. On Jan 28, 2012 this sequence version replaced NM_001130682.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1, and encodes isoform A. Variants 1, 2, 3, 4, and 8 all encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.279345.1, SRR1803613.65444.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124154.1
CDS	443..2515
Translation	MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	NM_001130682.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130682.2

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( NM_001130683.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124155.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	NM_001130683.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104083.3, DA518091.1, BX649180.1, AK226125.1 and CD671731.1. On Jun 2, 2019 this sequence version replaced NM_001130683.3. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1, and encodes isoform A. Variants 1, 2, 3, 4, and 8 all encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647200.1, SRR1803613.33278.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124155.1
CDS	560..2632
Translation	MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	NM_001130683.4
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130683.4

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( NM_001130683.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124155.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	NM_001130683.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA518091.1, BX649180.1, AC104083.3, AK226125.1 and CD671731.1. On Jan 28, 2012 this sequence version replaced NM_001130683.2. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1, and encodes isoform A. Variants 1, 2, 3, 4, and 8 all encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647200.1, SRR1803613.33278.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124155.1
CDS	411..2483
Translation	MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	NM_001130683.3
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130683.3

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( NM_001256449.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243378.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	NM_001256449.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK096588.1, Y15723.1, AC104083.3 and CD671731.1. Transcript Variant: This variant (8) differs in the 5' UTR, compared to variant 1, and encodes isoform A. Variants 1, 2, 3, 4, and 8 all encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK096588.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243378.1
CDS	374..2446
Translation	MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	NM_001256449.1
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256449.1

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( NM_000856.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_000847.2 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 1, mRNA.

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CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	NM_000856.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Y15723.1, AC104083.3 and CD671731.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_000856.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (A). Variants 1, 2, 3, 4, and 8 all encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.175812.1, Y15723.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000847.2
CDS	399..2471
Translation	MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	NM_000856.6
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000856.6

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( NM_001130682.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124154.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	NM_001130682.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-08
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA793192.1, Y15723.1, AC104083.3 and CD671731.1. On Jun 2, 2019 this sequence version replaced NM_001130682.2. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1, and encodes isoform A. Variants 1, 2, 3, 4, and 8 all encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.185004.1, SRR1803611.279345.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124154.1
CDS	305..2377
Translation	MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	NM_001130682.3
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130682.3

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( XM_006714197.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714260.1 Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X4, mRNA.

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CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	XM_006714197.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	guanylate cyclase soluble subunit alpha-3 isoform X1
Comment

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006714260.1

Translation

MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_006714197.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714197.1

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( XM_006714197.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714260.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	XM_006714197.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714197.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714260.1
CDS	167..2239
Translation	MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_006714197.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714197.2

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( XM_005262955.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263012.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	XM_005262955.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005262955.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263012.1
CDS	390..2462
Translation	MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_005262955.3
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005262955.3

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( XM_005262955.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263012.1 Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	XM_005262955.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005262955.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263012.1
CDS	405..2477
Translation	MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_005262955.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005262955.2

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( XM_006714196.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714259.1 Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	XM_006714196.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	guanylate cyclase soluble subunit alpha-3 isoform X1
Comment

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006714259.1

Translation

MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_006714196.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714196.1

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( XM_006714196.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714259.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	XM_006714196.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714196.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714259.1
CDS	392..2464
Translation	MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_006714196.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714196.2

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( XM_011531899.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530201.1 Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	XM_011531899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	guanylate cyclase soluble subunit alpha-3 isoform X1
Comment

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011530201.1

Translation

MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_011531899.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531899.1

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( XM_005262955.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263012.1 Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X1, mRNA.

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CloneID	OHu25145
Clone ID Related Accession (Same CDS sequence)	XM_006714197.1 , NM_000856.5 , NM_001130684.2 , NM_001130684.3 , NM_001256449.2 , XM_006714197.2 , NM_001130682.2 , XM_005262955.3 , XM_005262955.2 , XM_006714196.1 , NM_001130683.4 , XM_006714196.2 , XM_011531899.1 , XM_005262955.1 , NM_001130683.3 , NM_001256449.1 , NM_000856.6 , NM_001130682.3
Accession Version	XM_005262955.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	guanylate cyclase soluble subunit alpha-3 isoform X1
Comment

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005263012.1

Translation

MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_005262955.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005262955.1

ORF Insert Sequence:

ATGTTCTGCA CGAAGCTCAA GGATCTCAAG ATCACAGGAG AGTGTCCTTT CTCCTTACTG 
GCACCAGGTC AAGTTCCTAA CGAGTCTTCA GAGGAGGCAG CAGGAAGCTC AGAGAGCTGC 
AAAGCAACCG TGCCCATCTG TCAAGACATT CCTGAGAAGA ACATACAAGA AAGTCTTCCT 
CAAAGAAAAA CCAGTCGGAG CCGAGTCTAT CTTCACACTT TGGCAGAGAG TATTTGCAAA 
CTGATTTTCC CAGAGTTTGA ACGGCTGAAT GTTGCACTTC AGAGAACATT GGCAAAGCAC 
AAAATAAAAG AAAGCAGGAA ATCTTTGGAA AGAGAAGACT TTGAAAAAAC AATTGCAGAG 
CAAGCAGTTG CAGCAGGAGT TCCAGTGGAG GTTATCAAAG AATCTCTTGG TGAAGAGGTT 
TTTAAAATAT GTTACGAGGA AGATGAAAAC ATCCTTGGGG TGGTTGGAGG CACCCTTAAA 
GATTTTTTAA ACAGCTTCAG TACCCTTCTG AAACAGAGCA GCCATTGCCA AGAAGCAGGA 
AAAAGGGGCA GGCTTGAGGA CGCCTCCATT CTATGCCTGG ATAAGGAGGA TGATTTTCTA 
CATGTTTACT ACTTCTTCCC TAAGAGAACC ACCTCCCTGA TTCTTCCCGG CATCATAAAG 
GCAGCTGCTC ACGTATTATA TGAAACGGAA GTGGAAGTGT CGTTAATGCC TCCCTGCTTC 
CATAATGATT GCAGCGAGTT TGTGAATCAG CCCTACTTGT TGTACTCCGT TCACATGAAA 
AGCACCAAGC CATCCCTGTC CCCCAGCAAA CCCCAGTCCT CGCTGGTGAT TCCCACATCG 
CTATTCTGCA AGACATTTCC ATTCCATTTC ATGTTTGACA AAGATATGAC AATTCTGCAA 
TTTGGCAATG GCATCAGAAG GCTGATGAAC AGGAGAGACT TTCAAGGAAA GCCTAATTTT 
GAAGAATACT TTGAAATTCT GACTCCAAAA ATCAACCAGA CGTTTAGCGG GATCATGACT 
ATGTTGAATA TGCAGTTTGT TGTACGAGTG AGGAGATGGG ACAACTCTGT GAAAAAATCT 
TCAAGGGTTA TGGACCTCAA AGGCCAAATG ATCTACATTG TTGAATCCAG TGCAATCTTG 
TTTTTGGGGT CACCCTGTGT GGACAGATTA GAAGATTTTA CAGGACGAGG GCTCTACCTC 
TCAGACATCC CAATTCACAA TGCACTGAGG GATGTGGTCT TAATAGGGGA ACAAGCCCGA 
GCTCAAGATG GCCTGAAGAA GAGGCTGGGG AAGCTGAAGG CTACCCTTGA GCAAGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAAAAGACA GTAGACCTTC TGTGCTCCAT ATTTCCCTGT 
GAGGTTGCTC AGCAGCTGTG GCAAGGGCAA GTTGTGCAAG CCAAGAAGTT CAGTAATGTC 
ACCATGCTCT TCTCAGACAT CGTTGGGTTC ACTGCCATCT GCTCCCAGTG CTCACCGCTG 
CAGGTCATCA CCATGCTCAA TGCACTGTAC ACTCGCTTCG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATTGGCGAT GCCTATTGTG TAGCTGGGGG ATTACACAAA 
GAGAGTGATA CTCATGCTGT TCAGATAGCG CTGATGGCCC TGAAGATGAT GGAGCTCTCT 
GATGAAGTTA TGTCTCCCCA TGGAGAACCT ATCAAGATGC GAATTGGACT GCACTCTGGA 
TCAGTTTTTG CTGGCGTCGT TGGAGTTAAA ATGCCCCGTT ACTGTCTTTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAGTCCTGC AGTGTACCAC GAAAAATCAA TGTCAGCCCA 
ACAACTTACA GATTACTCAA AGACTGTCCT GGTTTCGTGT TTACCCCTCG ATCAAGGGAG 
GAACTTCCAC CAAACTTCCC TAGTGAAATC CCCGGAATCT GCCATTTTCT GGATGCTTAC 
CAACAAGGAA CAAACTCAAA ACCATGCTTC CAAAAGAAAG ATGTGGAAGA TGGCAATGCC 
AATTTTTTAG GCAAAGCATC AGGAATAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( NM_001130685.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124157.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu25175
Clone ID Related Accession (Same CDS sequence)	XM_006714198.2 , XM_005262956.2 , NM_001130685.2 , XM_005262956.3 , XM_011531900.2 , XM_005262957.2 , NM_001130685.3 , XM_005262957.3
Accession Version	NM_001130685.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-14
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA518091.1, AK226125.1, AC104083.3 and CD671731.1. On Jan 28, 2012 this sequence version replaced NM_001130685.1. Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (B) is shorter at the N-terminus compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK226125.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCCCT GCTTCCATAA TGATTGCAGC GAGTTTGTGA ATCAGCCCTA CTTGTTGTAC 
TCCGTTCACA TGAAAAGCAC CAAGCCATCC CTGTCCCCCA GCAAACCCCA GTCCTCGCTG 
GTGATTCCCA CATCGCTATT CTGCAAGACA TTTCCATTCC ATTTCATGTT TGACAAAGAT 
ATGACAATTC TGCAATTTGG CAATGGCATC AGAAGGCTGA TGAACAGGAG AGACTTTCAA 
GGAAAGCCTA ATTTTGAAGA ATACTTTGAA ATTCTGACTC CAAAAATCAA CCAGACGTTT 
AGCGGGATCA TGACTATGTT GAATATGCAG TTTGTTGTAC GAGTGAGGAG ATGGGACAAC 
TCTGTGAAAA AATCTTCAAG GGTTATGGAC CTCAAAGGCC AAATGATCTA CATTGTTGAA 
TCCAGTGCAA TCTTGTTTTT GGGGTCACCC TGTGTGGACA GATTAGAAGA TTTTACAGGA 
CGAGGGCTCT ACCTCTCAGA CATCCCAATT CACAATGCAC TGAGGGATGT GGTCTTAATA 
GGGGAACAAG CCCGAGCTCA AGATGGCCTG AAGAAGAGGC TGGGGAAGCT GAAGGCTACC 
CTTGAGCAAG CCCACCAAGC CCTGGAGGAG GAGAAGAAAA AGACAGTAGA CCTTCTGTGC 
TCCATATTTC CCTGTGAGGT TGCTCAGCAG CTGTGGCAAG GGCAAGTTGT GCAAGCCAAG 
AAGTTCAGTA ATGTCACCAT GCTCTTCTCA GACATCGTTG GGTTCACTGC CATCTGCTCC 
CAGTGCTCAC CGCTGCAGGT CATCACCATG CTCAATGCAC TGTACACTCG CTTCGACCAG 
CAGTGTGGAG AGCTGGATGT CTACAAGGTG GAGACCATTG GCGATGCCTA TTGTGTAGCT 
GGGGGATTAC ACAAAGAGAG TGATACTCAT GCTGTTCAGA TAGCGCTGAT GGCCCTGAAG 
ATGATGGAGC TCTCTGATGA AGTTATGTCT CCCCATGGAG AACCTATCAA GATGCGAATT 
GGACTGCACT CTGGATCAGT TTTTGCTGGC GTCGTTGGAG TTAAAATGCC CCGTTACTGT 
CTTTTTGGAA ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA 
ATCAATGTCA GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC 
CCTCGATCAA GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT 
TTTCTGGATG CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG 
GAAGATGGCA ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124157.1
CDS	937..2304
Translation	MPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	NM_001130685.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130685.2

ORF Insert Sequence:

ATGCCTCCCT GCTTCCATAA TGATTGCAGC GAGTTTGTGA ATCAGCCCTA CTTGTTGTAC 
TCCGTTCACA TGAAAAGCAC CAAGCCATCC CTGTCCCCCA GCAAACCCCA GTCCTCGCTG 
GTGATTCCCA CATCGCTATT CTGCAAGACA TTTCCATTCC ATTTCATGTT TGACAAAGAT 
ATGACAATTC TGCAATTTGG CAATGGCATC AGAAGGCTGA TGAACAGGAG AGACTTTCAA 
GGAAAGCCTA ATTTTGAAGA ATACTTTGAA ATTCTGACTC CAAAAATCAA CCAGACGTTT 
AGCGGGATCA TGACTATGTT GAATATGCAG TTTGTTGTAC GAGTGAGGAG ATGGGACAAC 
TCTGTGAAAA AATCTTCAAG GGTTATGGAC CTCAAAGGCC AAATGATCTA CATTGTTGAA 
TCCAGTGCAA TCTTGTTTTT GGGGTCACCC TGTGTGGACA GATTAGAAGA TTTTACAGGA 
CGAGGGCTCT ACCTCTCAGA CATCCCAATT CACAATGCAC TGAGGGATGT GGTCTTAATA 
GGGGAACAAG CCCGAGCTCA AGATGGCCTG AAGAAGAGGC TGGGGAAGCT GAAGGCTACC 
CTTGAGCAAG CCCACCAAGC CCTGGAGGAG GAGAAGAAAA AGACAGTAGA CCTTCTGTGC 
TCCATATTTC CCTGTGAGGT TGCTCAGCAG CTGTGGCAAG GGCAAGTTGT GCAAGCCAAG 
AAGTTCAGTA ATGTCACCAT GCTCTTCTCA GACATCGTTG GGTTCACTGC CATCTGCTCC 
CAGTGCTCAC CGCTGCAGGT CATCACCATG CTCAATGCAC TGTACACTCG CTTCGACCAG 
CAGTGTGGAG AGCTGGATGT CTACAAGGTG GAGACCATTG GCGATGCCTA TTGTGTAGCT 
GGGGGATTAC ACAAAGAGAG TGATACTCAT GCTGTTCAGA TAGCGCTGAT GGCCCTGAAG 
ATGATGGAGC TCTCTGATGA AGTTATGTCT CCCCATGGAG AACCTATCAA GATGCGAATT 
GGACTGCACT CTGGATCAGT TTTTGCTGGC GTCGTTGGAG TTAAAATGCC CCGTTACTGT 
CTTTTTGGAA ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA 
ATCAATGTCA GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC 
CCTCGATCAA GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT 
TTTCTGGATG CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG 
GAAGATGGCA ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( NM_001130685.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124157.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu25175
Clone ID Related Accession (Same CDS sequence)	XM_006714198.2 , XM_005262956.2 , NM_001130685.2 , XM_005262956.3 , XM_011531900.2 , XM_005262957.2 , NM_001130685.3 , XM_005262957.3
Accession Version	NM_001130685.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104083.3, DA518091.1, AK226125.1 and CD671731.1. On Jun 2, 2019 this sequence version replaced NM_001130685.2. Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (B) is shorter at the N-terminus compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK226125.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCCCT GCTTCCATAA TGATTGCAGC GAGTTTGTGA ATCAGCCCTA CTTGTTGTAC 
TCCGTTCACA TGAAAAGCAC CAAGCCATCC CTGTCCCCCA GCAAACCCCA GTCCTCGCTG 
GTGATTCCCA CATCGCTATT CTGCAAGACA TTTCCATTCC ATTTCATGTT TGACAAAGAT 
ATGACAATTC TGCAATTTGG CAATGGCATC AGAAGGCTGA TGAACAGGAG AGACTTTCAA 
GGAAAGCCTA ATTTTGAAGA ATACTTTGAA ATTCTGACTC CAAAAATCAA CCAGACGTTT 
AGCGGGATCA TGACTATGTT GAATATGCAG TTTGTTGTAC GAGTGAGGAG ATGGGACAAC 
TCTGTGAAAA AATCTTCAAG GGTTATGGAC CTCAAAGGCC AAATGATCTA CATTGTTGAA 
TCCAGTGCAA TCTTGTTTTT GGGGTCACCC TGTGTGGACA GATTAGAAGA TTTTACAGGA 
CGAGGGCTCT ACCTCTCAGA CATCCCAATT CACAATGCAC TGAGGGATGT GGTCTTAATA 
GGGGAACAAG CCCGAGCTCA AGATGGCCTG AAGAAGAGGC TGGGGAAGCT GAAGGCTACC 
CTTGAGCAAG CCCACCAAGC CCTGGAGGAG GAGAAGAAAA AGACAGTAGA CCTTCTGTGC 
TCCATATTTC CCTGTGAGGT TGCTCAGCAG CTGTGGCAAG GGCAAGTTGT GCAAGCCAAG 
AAGTTCAGTA ATGTCACCAT GCTCTTCTCA GACATCGTTG GGTTCACTGC CATCTGCTCC 
CAGTGCTCAC CGCTGCAGGT CATCACCATG CTCAATGCAC TGTACACTCG CTTCGACCAG 
CAGTGTGGAG AGCTGGATGT CTACAAGGTG GAGACCATTG GCGATGCCTA TTGTGTAGCT 
GGGGGATTAC ACAAAGAGAG TGATACTCAT GCTGTTCAGA TAGCGCTGAT GGCCCTGAAG 
ATGATGGAGC TCTCTGATGA AGTTATGTCT CCCCATGGAG AACCTATCAA GATGCGAATT 
GGACTGCACT CTGGATCAGT TTTTGCTGGC GTCGTTGGAG TTAAAATGCC CCGTTACTGT 
CTTTTTGGAA ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA 
ATCAATGTCA GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC 
CCTCGATCAA GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT 
TTTCTGGATG CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG 
GAAGATGGCA ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124157.1
CDS	1086..2453
Translation	MPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	NM_001130685.3
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130685.3

ORF Insert Sequence:

ATGCCTCCCT GCTTCCATAA TGATTGCAGC GAGTTTGTGA ATCAGCCCTA CTTGTTGTAC 
TCCGTTCACA TGAAAAGCAC CAAGCCATCC CTGTCCCCCA GCAAACCCCA GTCCTCGCTG 
GTGATTCCCA CATCGCTATT CTGCAAGACA TTTCCATTCC ATTTCATGTT TGACAAAGAT 
ATGACAATTC TGCAATTTGG CAATGGCATC AGAAGGCTGA TGAACAGGAG AGACTTTCAA 
GGAAAGCCTA ATTTTGAAGA ATACTTTGAA ATTCTGACTC CAAAAATCAA CCAGACGTTT 
AGCGGGATCA TGACTATGTT GAATATGCAG TTTGTTGTAC GAGTGAGGAG ATGGGACAAC 
TCTGTGAAAA AATCTTCAAG GGTTATGGAC CTCAAAGGCC AAATGATCTA CATTGTTGAA 
TCCAGTGCAA TCTTGTTTTT GGGGTCACCC TGTGTGGACA GATTAGAAGA TTTTACAGGA 
CGAGGGCTCT ACCTCTCAGA CATCCCAATT CACAATGCAC TGAGGGATGT GGTCTTAATA 
GGGGAACAAG CCCGAGCTCA AGATGGCCTG AAGAAGAGGC TGGGGAAGCT GAAGGCTACC 
CTTGAGCAAG CCCACCAAGC CCTGGAGGAG GAGAAGAAAA AGACAGTAGA CCTTCTGTGC 
TCCATATTTC CCTGTGAGGT TGCTCAGCAG CTGTGGCAAG GGCAAGTTGT GCAAGCCAAG 
AAGTTCAGTA ATGTCACCAT GCTCTTCTCA GACATCGTTG GGTTCACTGC CATCTGCTCC 
CAGTGCTCAC CGCTGCAGGT CATCACCATG CTCAATGCAC TGTACACTCG CTTCGACCAG 
CAGTGTGGAG AGCTGGATGT CTACAAGGTG GAGACCATTG GCGATGCCTA TTGTGTAGCT 
GGGGGATTAC ACAAAGAGAG TGATACTCAT GCTGTTCAGA TAGCGCTGAT GGCCCTGAAG 
ATGATGGAGC TCTCTGATGA AGTTATGTCT CCCCATGGAG AACCTATCAA GATGCGAATT 
GGACTGCACT CTGGATCAGT TTTTGCTGGC GTCGTTGGAG TTAAAATGCC CCGTTACTGT 
CTTTTTGGAA ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA 
ATCAATGTCA GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC 
CCTCGATCAA GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT 
TTTCTGGATG CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG 
GAAGATGGCA ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( XM_006714198.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714261.1 Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu25175
Clone ID Related Accession (Same CDS sequence)	XM_006714198.2 , XM_005262956.2 , NM_001130685.2 , XM_005262956.3 , XM_011531900.2 , XM_005262957.2 , NM_001130685.3 , XM_005262957.3
Accession Version	XM_006714198.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714198.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCCT GCTTCCATAA TGATTGCAGC GAGTTTGTGA ATCAGCCCTA CTTGTTGTAC 
TCCGTTCACA TGAAAAGCAC CAAGCCATCC CTGTCCCCCA GCAAACCCCA GTCCTCGCTG 
GTGATTCCCA CATCGCTATT CTGCAAGACA TTTCCATTCC ATTTCATGTT TGACAAAGAT 
ATGACAATTC TGCAATTTGG CAATGGCATC AGAAGGCTGA TGAACAGGAG AGACTTTCAA 
GGAAAGCCTA ATTTTGAAGA ATACTTTGAA ATTCTGACTC CAAAAATCAA CCAGACGTTT 
AGCGGGATCA TGACTATGTT GAATATGCAG TTTGTTGTAC GAGTGAGGAG ATGGGACAAC 
TCTGTGAAAA AATCTTCAAG GGTTATGGAC CTCAAAGGCC AAATGATCTA CATTGTTGAA 
TCCAGTGCAA TCTTGTTTTT GGGGTCACCC TGTGTGGACA GATTAGAAGA TTTTACAGGA 
CGAGGGCTCT ACCTCTCAGA CATCCCAATT CACAATGCAC TGAGGGATGT GGTCTTAATA 
GGGGAACAAG CCCGAGCTCA AGATGGCCTG AAGAAGAGGC TGGGGAAGCT GAAGGCTACC 
CTTGAGCAAG CCCACCAAGC CCTGGAGGAG GAGAAGAAAA AGACAGTAGA CCTTCTGTGC 
TCCATATTTC CCTGTGAGGT TGCTCAGCAG CTGTGGCAAG GGCAAGTTGT GCAAGCCAAG 
AAGTTCAGTA ATGTCACCAT GCTCTTCTCA GACATCGTTG GGTTCACTGC CATCTGCTCC 
CAGTGCTCAC CGCTGCAGGT CATCACCATG CTCAATGCAC TGTACACTCG CTTCGACCAG 
CAGTGTGGAG AGCTGGATGT CTACAAGGTG GAGACCATTG GCGATGCCTA TTGTGTAGCT 
GGGGGATTAC ACAAAGAGAG TGATACTCAT GCTGTTCAGA TAGCGCTGAT GGCCCTGAAG 
ATGATGGAGC TCTCTGATGA AGTTATGTCT CCCCATGGAG AACCTATCAA GATGCGAATT 
GGACTGCACT CTGGATCAGT TTTTGCTGGC GTCGTTGGAG TTAAAATGCC CCGTTACTGT 
CTTTTTGGAA ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA 
ATCAATGTCA GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC 
CCTCGATCAA GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT 
TTTCTGGATG CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG 
GAAGATGGCA ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714261.1
CDS	757..2124
Translation	MPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_006714198.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714198.2

ORF Insert Sequence:

ATGCCTCCCT GCTTCCATAA TGATTGCAGC GAGTTTGTGA ATCAGCCCTA CTTGTTGTAC 
TCCGTTCACA TGAAAAGCAC CAAGCCATCC CTGTCCCCCA GCAAACCCCA GTCCTCGCTG 
GTGATTCCCA CATCGCTATT CTGCAAGACA TTTCCATTCC ATTTCATGTT TGACAAAGAT 
ATGACAATTC TGCAATTTGG CAATGGCATC AGAAGGCTGA TGAACAGGAG AGACTTTCAA 
GGAAAGCCTA ATTTTGAAGA ATACTTTGAA ATTCTGACTC CAAAAATCAA CCAGACGTTT 
AGCGGGATCA TGACTATGTT GAATATGCAG TTTGTTGTAC GAGTGAGGAG ATGGGACAAC 
TCTGTGAAAA AATCTTCAAG GGTTATGGAC CTCAAAGGCC AAATGATCTA CATTGTTGAA 
TCCAGTGCAA TCTTGTTTTT GGGGTCACCC TGTGTGGACA GATTAGAAGA TTTTACAGGA 
CGAGGGCTCT ACCTCTCAGA CATCCCAATT CACAATGCAC TGAGGGATGT GGTCTTAATA 
GGGGAACAAG CCCGAGCTCA AGATGGCCTG AAGAAGAGGC TGGGGAAGCT GAAGGCTACC 
CTTGAGCAAG CCCACCAAGC CCTGGAGGAG GAGAAGAAAA AGACAGTAGA CCTTCTGTGC 
TCCATATTTC CCTGTGAGGT TGCTCAGCAG CTGTGGCAAG GGCAAGTTGT GCAAGCCAAG 
AAGTTCAGTA ATGTCACCAT GCTCTTCTCA GACATCGTTG GGTTCACTGC CATCTGCTCC 
CAGTGCTCAC CGCTGCAGGT CATCACCATG CTCAATGCAC TGTACACTCG CTTCGACCAG 
CAGTGTGGAG AGCTGGATGT CTACAAGGTG GAGACCATTG GCGATGCCTA TTGTGTAGCT 
GGGGGATTAC ACAAAGAGAG TGATACTCAT GCTGTTCAGA TAGCGCTGAT GGCCCTGAAG 
ATGATGGAGC TCTCTGATGA AGTTATGTCT CCCCATGGAG AACCTATCAA GATGCGAATT 
GGACTGCACT CTGGATCAGT TTTTGCTGGC GTCGTTGGAG TTAAAATGCC CCGTTACTGT 
CTTTTTGGAA ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA 
ATCAATGTCA GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC 
CCTCGATCAA GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT 
TTTCTGGATG CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG 
GAAGATGGCA ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( XM_005262956.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263013.1 Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu25175
Clone ID Related Accession (Same CDS sequence)	XM_006714198.2 , XM_005262956.2 , NM_001130685.2 , XM_005262956.3 , XM_011531900.2 , XM_005262957.2 , NM_001130685.3 , XM_005262957.3
Accession Version	XM_005262956.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005262956.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCCT GCTTCCATAA TGATTGCAGC GAGTTTGTGA ATCAGCCCTA CTTGTTGTAC 
TCCGTTCACA TGAAAAGCAC CAAGCCATCC CTGTCCCCCA GCAAACCCCA GTCCTCGCTG 
GTGATTCCCA CATCGCTATT CTGCAAGACA TTTCCATTCC ATTTCATGTT TGACAAAGAT 
ATGACAATTC TGCAATTTGG CAATGGCATC AGAAGGCTGA TGAACAGGAG AGACTTTCAA 
GGAAAGCCTA ATTTTGAAGA ATACTTTGAA ATTCTGACTC CAAAAATCAA CCAGACGTTT 
AGCGGGATCA TGACTATGTT GAATATGCAG TTTGTTGTAC GAGTGAGGAG ATGGGACAAC 
TCTGTGAAAA AATCTTCAAG GGTTATGGAC CTCAAAGGCC AAATGATCTA CATTGTTGAA 
TCCAGTGCAA TCTTGTTTTT GGGGTCACCC TGTGTGGACA GATTAGAAGA TTTTACAGGA 
CGAGGGCTCT ACCTCTCAGA CATCCCAATT CACAATGCAC TGAGGGATGT GGTCTTAATA 
GGGGAACAAG CCCGAGCTCA AGATGGCCTG AAGAAGAGGC TGGGGAAGCT GAAGGCTACC 
CTTGAGCAAG CCCACCAAGC CCTGGAGGAG GAGAAGAAAA AGACAGTAGA CCTTCTGTGC 
TCCATATTTC CCTGTGAGGT TGCTCAGCAG CTGTGGCAAG GGCAAGTTGT GCAAGCCAAG 
AAGTTCAGTA ATGTCACCAT GCTCTTCTCA GACATCGTTG GGTTCACTGC CATCTGCTCC 
CAGTGCTCAC CGCTGCAGGT CATCACCATG CTCAATGCAC TGTACACTCG CTTCGACCAG 
CAGTGTGGAG AGCTGGATGT CTACAAGGTG GAGACCATTG GCGATGCCTA TTGTGTAGCT 
GGGGGATTAC ACAAAGAGAG TGATACTCAT GCTGTTCAGA TAGCGCTGAT GGCCCTGAAG 
ATGATGGAGC TCTCTGATGA AGTTATGTCT CCCCATGGAG AACCTATCAA GATGCGAATT 
GGACTGCACT CTGGATCAGT TTTTGCTGGC GTCGTTGGAG TTAAAATGCC CCGTTACTGT 
CTTTTTGGAA ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA 
ATCAATGTCA GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC 
CCTCGATCAA GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT 
TTTCTGGATG CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG 
GAAGATGGCA ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263013.1
CDS	925..2292
Translation	MPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_005262956.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005262956.2

ORF Insert Sequence:

ATGCCTCCCT GCTTCCATAA TGATTGCAGC GAGTTTGTGA ATCAGCCCTA CTTGTTGTAC 
TCCGTTCACA TGAAAAGCAC CAAGCCATCC CTGTCCCCCA GCAAACCCCA GTCCTCGCTG 
GTGATTCCCA CATCGCTATT CTGCAAGACA TTTCCATTCC ATTTCATGTT TGACAAAGAT 
ATGACAATTC TGCAATTTGG CAATGGCATC AGAAGGCTGA TGAACAGGAG AGACTTTCAA 
GGAAAGCCTA ATTTTGAAGA ATACTTTGAA ATTCTGACTC CAAAAATCAA CCAGACGTTT 
AGCGGGATCA TGACTATGTT GAATATGCAG TTTGTTGTAC GAGTGAGGAG ATGGGACAAC 
TCTGTGAAAA AATCTTCAAG GGTTATGGAC CTCAAAGGCC AAATGATCTA CATTGTTGAA 
TCCAGTGCAA TCTTGTTTTT GGGGTCACCC TGTGTGGACA GATTAGAAGA TTTTACAGGA 
CGAGGGCTCT ACCTCTCAGA CATCCCAATT CACAATGCAC TGAGGGATGT GGTCTTAATA 
GGGGAACAAG CCCGAGCTCA AGATGGCCTG AAGAAGAGGC TGGGGAAGCT GAAGGCTACC 
CTTGAGCAAG CCCACCAAGC CCTGGAGGAG GAGAAGAAAA AGACAGTAGA CCTTCTGTGC 
TCCATATTTC CCTGTGAGGT TGCTCAGCAG CTGTGGCAAG GGCAAGTTGT GCAAGCCAAG 
AAGTTCAGTA ATGTCACCAT GCTCTTCTCA GACATCGTTG GGTTCACTGC CATCTGCTCC 
CAGTGCTCAC CGCTGCAGGT CATCACCATG CTCAATGCAC TGTACACTCG CTTCGACCAG 
CAGTGTGGAG AGCTGGATGT CTACAAGGTG GAGACCATTG GCGATGCCTA TTGTGTAGCT 
GGGGGATTAC ACAAAGAGAG TGATACTCAT GCTGTTCAGA TAGCGCTGAT GGCCCTGAAG 
ATGATGGAGC TCTCTGATGA AGTTATGTCT CCCCATGGAG AACCTATCAA GATGCGAATT 
GGACTGCACT CTGGATCAGT TTTTGCTGGC GTCGTTGGAG TTAAAATGCC CCGTTACTGT 
CTTTTTGGAA ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA 
ATCAATGTCA GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC 
CCTCGATCAA GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT 
TTTCTGGATG CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG 
GAAGATGGCA ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( XM_005262956.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263013.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu25175
Clone ID Related Accession (Same CDS sequence)	XM_006714198.2 , XM_005262956.2 , NM_001130685.2 , XM_005262956.3 , XM_011531900.2 , XM_005262957.2 , NM_001130685.3 , XM_005262957.3
Accession Version	XM_005262956.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005262956.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCCT GCTTCCATAA TGATTGCAGC GAGTTTGTGA ATCAGCCCTA CTTGTTGTAC 
TCCGTTCACA TGAAAAGCAC CAAGCCATCC CTGTCCCCCA GCAAACCCCA GTCCTCGCTG 
GTGATTCCCA CATCGCTATT CTGCAAGACA TTTCCATTCC ATTTCATGTT TGACAAAGAT 
ATGACAATTC TGCAATTTGG CAATGGCATC AGAAGGCTGA TGAACAGGAG AGACTTTCAA 
GGAAAGCCTA ATTTTGAAGA ATACTTTGAA ATTCTGACTC CAAAAATCAA CCAGACGTTT 
AGCGGGATCA TGACTATGTT GAATATGCAG TTTGTTGTAC GAGTGAGGAG ATGGGACAAC 
TCTGTGAAAA AATCTTCAAG GGTTATGGAC CTCAAAGGCC AAATGATCTA CATTGTTGAA 
TCCAGTGCAA TCTTGTTTTT GGGGTCACCC TGTGTGGACA GATTAGAAGA TTTTACAGGA 
CGAGGGCTCT ACCTCTCAGA CATCCCAATT CACAATGCAC TGAGGGATGT GGTCTTAATA 
GGGGAACAAG CCCGAGCTCA AGATGGCCTG AAGAAGAGGC TGGGGAAGCT GAAGGCTACC 
CTTGAGCAAG CCCACCAAGC CCTGGAGGAG GAGAAGAAAA AGACAGTAGA CCTTCTGTGC 
TCCATATTTC CCTGTGAGGT TGCTCAGCAG CTGTGGCAAG GGCAAGTTGT GCAAGCCAAG 
AAGTTCAGTA ATGTCACCAT GCTCTTCTCA GACATCGTTG GGTTCACTGC CATCTGCTCC 
CAGTGCTCAC CGCTGCAGGT CATCACCATG CTCAATGCAC TGTACACTCG CTTCGACCAG 
CAGTGTGGAG AGCTGGATGT CTACAAGGTG GAGACCATTG GCGATGCCTA TTGTGTAGCT 
GGGGGATTAC ACAAAGAGAG TGATACTCAT GCTGTTCAGA TAGCGCTGAT GGCCCTGAAG 
ATGATGGAGC TCTCTGATGA AGTTATGTCT CCCCATGGAG AACCTATCAA GATGCGAATT 
GGACTGCACT CTGGATCAGT TTTTGCTGGC GTCGTTGGAG TTAAAATGCC CCGTTACTGT 
CTTTTTGGAA ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA 
ATCAATGTCA GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC 
CCTCGATCAA GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT 
TTTCTGGATG CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG 
GAAGATGGCA ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263013.1
CDS	1048..2415
Translation	MPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_005262956.3
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005262956.3

ORF Insert Sequence:

ATGCCTCCCT GCTTCCATAA TGATTGCAGC GAGTTTGTGA ATCAGCCCTA CTTGTTGTAC 
TCCGTTCACA TGAAAAGCAC CAAGCCATCC CTGTCCCCCA GCAAACCCCA GTCCTCGCTG 
GTGATTCCCA CATCGCTATT CTGCAAGACA TTTCCATTCC ATTTCATGTT TGACAAAGAT 
ATGACAATTC TGCAATTTGG CAATGGCATC AGAAGGCTGA TGAACAGGAG AGACTTTCAA 
GGAAAGCCTA ATTTTGAAGA ATACTTTGAA ATTCTGACTC CAAAAATCAA CCAGACGTTT 
AGCGGGATCA TGACTATGTT GAATATGCAG TTTGTTGTAC GAGTGAGGAG ATGGGACAAC 
TCTGTGAAAA AATCTTCAAG GGTTATGGAC CTCAAAGGCC AAATGATCTA CATTGTTGAA 
TCCAGTGCAA TCTTGTTTTT GGGGTCACCC TGTGTGGACA GATTAGAAGA TTTTACAGGA 
CGAGGGCTCT ACCTCTCAGA CATCCCAATT CACAATGCAC TGAGGGATGT GGTCTTAATA 
GGGGAACAAG CCCGAGCTCA AGATGGCCTG AAGAAGAGGC TGGGGAAGCT GAAGGCTACC 
CTTGAGCAAG CCCACCAAGC CCTGGAGGAG GAGAAGAAAA AGACAGTAGA CCTTCTGTGC 
TCCATATTTC CCTGTGAGGT TGCTCAGCAG CTGTGGCAAG GGCAAGTTGT GCAAGCCAAG 
AAGTTCAGTA ATGTCACCAT GCTCTTCTCA GACATCGTTG GGTTCACTGC CATCTGCTCC 
CAGTGCTCAC CGCTGCAGGT CATCACCATG CTCAATGCAC TGTACACTCG CTTCGACCAG 
CAGTGTGGAG AGCTGGATGT CTACAAGGTG GAGACCATTG GCGATGCCTA TTGTGTAGCT 
GGGGGATTAC ACAAAGAGAG TGATACTCAT GCTGTTCAGA TAGCGCTGAT GGCCCTGAAG 
ATGATGGAGC TCTCTGATGA AGTTATGTCT CCCCATGGAG AACCTATCAA GATGCGAATT 
GGACTGCACT CTGGATCAGT TTTTGCTGGC GTCGTTGGAG TTAAAATGCC CCGTTACTGT 
CTTTTTGGAA ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA 
ATCAATGTCA GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC 
CCTCGATCAA GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT 
TTTCTGGATG CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG 
GAAGATGGCA ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( XM_011531900.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530202.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu25175
Clone ID Related Accession (Same CDS sequence)	XM_006714198.2 , XM_005262956.2 , NM_001130685.2 , XM_005262956.3 , XM_011531900.2 , XM_005262957.2 , NM_001130685.3 , XM_005262957.3
Accession Version	XM_011531900.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011531900.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCCT GCTTCCATAA TGATTGCAGC GAGTTTGTGA ATCAGCCCTA CTTGTTGTAC 
TCCGTTCACA TGAAAAGCAC CAAGCCATCC CTGTCCCCCA GCAAACCCCA GTCCTCGCTG 
GTGATTCCCA CATCGCTATT CTGCAAGACA TTTCCATTCC ATTTCATGTT TGACAAAGAT 
ATGACAATTC TGCAATTTGG CAATGGCATC AGAAGGCTGA TGAACAGGAG AGACTTTCAA 
GGAAAGCCTA ATTTTGAAGA ATACTTTGAA ATTCTGACTC CAAAAATCAA CCAGACGTTT 
AGCGGGATCA TGACTATGTT GAATATGCAG TTTGTTGTAC GAGTGAGGAG ATGGGACAAC 
TCTGTGAAAA AATCTTCAAG GGTTATGGAC CTCAAAGGCC AAATGATCTA CATTGTTGAA 
TCCAGTGCAA TCTTGTTTTT GGGGTCACCC TGTGTGGACA GATTAGAAGA TTTTACAGGA 
CGAGGGCTCT ACCTCTCAGA CATCCCAATT CACAATGCAC TGAGGGATGT GGTCTTAATA 
GGGGAACAAG CCCGAGCTCA AGATGGCCTG AAGAAGAGGC TGGGGAAGCT GAAGGCTACC 
CTTGAGCAAG CCCACCAAGC CCTGGAGGAG GAGAAGAAAA AGACAGTAGA CCTTCTGTGC 
TCCATATTTC CCTGTGAGGT TGCTCAGCAG CTGTGGCAAG GGCAAGTTGT GCAAGCCAAG 
AAGTTCAGTA ATGTCACCAT GCTCTTCTCA GACATCGTTG GGTTCACTGC CATCTGCTCC 
CAGTGCTCAC CGCTGCAGGT CATCACCATG CTCAATGCAC TGTACACTCG CTTCGACCAG 
CAGTGTGGAG AGCTGGATGT CTACAAGGTG GAGACCATTG GCGATGCCTA TTGTGTAGCT 
GGGGGATTAC ACAAAGAGAG TGATACTCAT GCTGTTCAGA TAGCGCTGAT GGCCCTGAAG 
ATGATGGAGC TCTCTGATGA AGTTATGTCT CCCCATGGAG AACCTATCAA GATGCGAATT 
GGACTGCACT CTGGATCAGT TTTTGCTGGC GTCGTTGGAG TTAAAATGCC CCGTTACTGT 
CTTTTTGGAA ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA 
ATCAATGTCA GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC 
CCTCGATCAA GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT 
TTTCTGGATG CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG 
GAAGATGGCA ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530202.1
CDS	836..2203
Translation	MPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_011531900.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531900.2

ORF Insert Sequence:

ATGCCTCCCT GCTTCCATAA TGATTGCAGC GAGTTTGTGA ATCAGCCCTA CTTGTTGTAC 
TCCGTTCACA TGAAAAGCAC CAAGCCATCC CTGTCCCCCA GCAAACCCCA GTCCTCGCTG 
GTGATTCCCA CATCGCTATT CTGCAAGACA TTTCCATTCC ATTTCATGTT TGACAAAGAT 
ATGACAATTC TGCAATTTGG CAATGGCATC AGAAGGCTGA TGAACAGGAG AGACTTTCAA 
GGAAAGCCTA ATTTTGAAGA ATACTTTGAA ATTCTGACTC CAAAAATCAA CCAGACGTTT 
AGCGGGATCA TGACTATGTT GAATATGCAG TTTGTTGTAC GAGTGAGGAG ATGGGACAAC 
TCTGTGAAAA AATCTTCAAG GGTTATGGAC CTCAAAGGCC AAATGATCTA CATTGTTGAA 
TCCAGTGCAA TCTTGTTTTT GGGGTCACCC TGTGTGGACA GATTAGAAGA TTTTACAGGA 
CGAGGGCTCT ACCTCTCAGA CATCCCAATT CACAATGCAC TGAGGGATGT GGTCTTAATA 
GGGGAACAAG CCCGAGCTCA AGATGGCCTG AAGAAGAGGC TGGGGAAGCT GAAGGCTACC 
CTTGAGCAAG CCCACCAAGC CCTGGAGGAG GAGAAGAAAA AGACAGTAGA CCTTCTGTGC 
TCCATATTTC CCTGTGAGGT TGCTCAGCAG CTGTGGCAAG GGCAAGTTGT GCAAGCCAAG 
AAGTTCAGTA ATGTCACCAT GCTCTTCTCA GACATCGTTG GGTTCACTGC CATCTGCTCC 
CAGTGCTCAC CGCTGCAGGT CATCACCATG CTCAATGCAC TGTACACTCG CTTCGACCAG 
CAGTGTGGAG AGCTGGATGT CTACAAGGTG GAGACCATTG GCGATGCCTA TTGTGTAGCT 
GGGGGATTAC ACAAAGAGAG TGATACTCAT GCTGTTCAGA TAGCGCTGAT GGCCCTGAAG 
ATGATGGAGC TCTCTGATGA AGTTATGTCT CCCCATGGAG AACCTATCAA GATGCGAATT 
GGACTGCACT CTGGATCAGT TTTTGCTGGC GTCGTTGGAG TTAAAATGCC CCGTTACTGT 
CTTTTTGGAA ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA 
ATCAATGTCA GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC 
CCTCGATCAA GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT 
TTTCTGGATG CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG 
GAAGATGGCA ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( XM_005262957.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263014.1 Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X5, mRNA.

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CloneID	OHu25175
Clone ID Related Accession (Same CDS sequence)	XM_006714198.2 , XM_005262956.2 , NM_001130685.2 , XM_005262956.3 , XM_011531900.2 , XM_005262957.2 , NM_001130685.3 , XM_005262957.3
Accession Version	XM_005262957.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005262957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCCT GCTTCCATAA TGATTGCAGC GAGTTTGTGA ATCAGCCCTA CTTGTTGTAC 
TCCGTTCACA TGAAAAGCAC CAAGCCATCC CTGTCCCCCA GCAAACCCCA GTCCTCGCTG 
GTGATTCCCA CATCGCTATT CTGCAAGACA TTTCCATTCC ATTTCATGTT TGACAAAGAT 
ATGACAATTC TGCAATTTGG CAATGGCATC AGAAGGCTGA TGAACAGGAG AGACTTTCAA 
GGAAAGCCTA ATTTTGAAGA ATACTTTGAA ATTCTGACTC CAAAAATCAA CCAGACGTTT 
AGCGGGATCA TGACTATGTT GAATATGCAG TTTGTTGTAC GAGTGAGGAG ATGGGACAAC 
TCTGTGAAAA AATCTTCAAG GGTTATGGAC CTCAAAGGCC AAATGATCTA CATTGTTGAA 
TCCAGTGCAA TCTTGTTTTT GGGGTCACCC TGTGTGGACA GATTAGAAGA TTTTACAGGA 
CGAGGGCTCT ACCTCTCAGA CATCCCAATT CACAATGCAC TGAGGGATGT GGTCTTAATA 
GGGGAACAAG CCCGAGCTCA AGATGGCCTG AAGAAGAGGC TGGGGAAGCT GAAGGCTACC 
CTTGAGCAAG CCCACCAAGC CCTGGAGGAG GAGAAGAAAA AGACAGTAGA CCTTCTGTGC 
TCCATATTTC CCTGTGAGGT TGCTCAGCAG CTGTGGCAAG GGCAAGTTGT GCAAGCCAAG 
AAGTTCAGTA ATGTCACCAT GCTCTTCTCA GACATCGTTG GGTTCACTGC CATCTGCTCC 
CAGTGCTCAC CGCTGCAGGT CATCACCATG CTCAATGCAC TGTACACTCG CTTCGACCAG 
CAGTGTGGAG AGCTGGATGT CTACAAGGTG GAGACCATTG GCGATGCCTA TTGTGTAGCT 
GGGGGATTAC ACAAAGAGAG TGATACTCAT GCTGTTCAGA TAGCGCTGAT GGCCCTGAAG 
ATGATGGAGC TCTCTGATGA AGTTATGTCT CCCCATGGAG AACCTATCAA GATGCGAATT 
GGACTGCACT CTGGATCAGT TTTTGCTGGC GTCGTTGGAG TTAAAATGCC CCGTTACTGT 
CTTTTTGGAA ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA 
ATCAATGTCA GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC 
CCTCGATCAA GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT 
TTTCTGGATG CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG 
GAAGATGGCA ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263014.1
CDS	1030..2397
Translation	MPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_005262957.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005262957.2

ORF Insert Sequence:

ATGCCTCCCT GCTTCCATAA TGATTGCAGC GAGTTTGTGA ATCAGCCCTA CTTGTTGTAC 
TCCGTTCACA TGAAAAGCAC CAAGCCATCC CTGTCCCCCA GCAAACCCCA GTCCTCGCTG 
GTGATTCCCA CATCGCTATT CTGCAAGACA TTTCCATTCC ATTTCATGTT TGACAAAGAT 
ATGACAATTC TGCAATTTGG CAATGGCATC AGAAGGCTGA TGAACAGGAG AGACTTTCAA 
GGAAAGCCTA ATTTTGAAGA ATACTTTGAA ATTCTGACTC CAAAAATCAA CCAGACGTTT 
AGCGGGATCA TGACTATGTT GAATATGCAG TTTGTTGTAC GAGTGAGGAG ATGGGACAAC 
TCTGTGAAAA AATCTTCAAG GGTTATGGAC CTCAAAGGCC AAATGATCTA CATTGTTGAA 
TCCAGTGCAA TCTTGTTTTT GGGGTCACCC TGTGTGGACA GATTAGAAGA TTTTACAGGA 
CGAGGGCTCT ACCTCTCAGA CATCCCAATT CACAATGCAC TGAGGGATGT GGTCTTAATA 
GGGGAACAAG CCCGAGCTCA AGATGGCCTG AAGAAGAGGC TGGGGAAGCT GAAGGCTACC 
CTTGAGCAAG CCCACCAAGC CCTGGAGGAG GAGAAGAAAA AGACAGTAGA CCTTCTGTGC 
TCCATATTTC CCTGTGAGGT TGCTCAGCAG CTGTGGCAAG GGCAAGTTGT GCAAGCCAAG 
AAGTTCAGTA ATGTCACCAT GCTCTTCTCA GACATCGTTG GGTTCACTGC CATCTGCTCC 
CAGTGCTCAC CGCTGCAGGT CATCACCATG CTCAATGCAC TGTACACTCG CTTCGACCAG 
CAGTGTGGAG AGCTGGATGT CTACAAGGTG GAGACCATTG GCGATGCCTA TTGTGTAGCT 
GGGGGATTAC ACAAAGAGAG TGATACTCAT GCTGTTCAGA TAGCGCTGAT GGCCCTGAAG 
ATGATGGAGC TCTCTGATGA AGTTATGTCT CCCCATGGAG AACCTATCAA GATGCGAATT 
GGACTGCACT CTGGATCAGT TTTTGCTGGC GTCGTTGGAG TTAAAATGCC CCGTTACTGT 
CTTTTTGGAA ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA 
ATCAATGTCA GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC 
CCTCGATCAA GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT 
TTTCTGGATG CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG 
GAAGATGGCA ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A1 ( XM_005262957.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263014.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu25175
Clone ID Related Accession (Same CDS sequence)	XM_006714198.2 , XM_005262956.2 , NM_001130685.2 , XM_005262956.3 , XM_011531900.2 , XM_005262957.2 , NM_001130685.3 , XM_005262957.3
Accession Version	XM_005262957.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005262957.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCCT GCTTCCATAA TGATTGCAGC GAGTTTGTGA ATCAGCCCTA CTTGTTGTAC 
TCCGTTCACA TGAAAAGCAC CAAGCCATCC CTGTCCCCCA GCAAACCCCA GTCCTCGCTG 
GTGATTCCCA CATCGCTATT CTGCAAGACA TTTCCATTCC ATTTCATGTT TGACAAAGAT 
ATGACAATTC TGCAATTTGG CAATGGCATC AGAAGGCTGA TGAACAGGAG AGACTTTCAA 
GGAAAGCCTA ATTTTGAAGA ATACTTTGAA ATTCTGACTC CAAAAATCAA CCAGACGTTT 
AGCGGGATCA TGACTATGTT GAATATGCAG TTTGTTGTAC GAGTGAGGAG ATGGGACAAC 
TCTGTGAAAA AATCTTCAAG GGTTATGGAC CTCAAAGGCC AAATGATCTA CATTGTTGAA 
TCCAGTGCAA TCTTGTTTTT GGGGTCACCC TGTGTGGACA GATTAGAAGA TTTTACAGGA 
CGAGGGCTCT ACCTCTCAGA CATCCCAATT CACAATGCAC TGAGGGATGT GGTCTTAATA 
GGGGAACAAG CCCGAGCTCA AGATGGCCTG AAGAAGAGGC TGGGGAAGCT GAAGGCTACC 
CTTGAGCAAG CCCACCAAGC CCTGGAGGAG GAGAAGAAAA AGACAGTAGA CCTTCTGTGC 
TCCATATTTC CCTGTGAGGT TGCTCAGCAG CTGTGGCAAG GGCAAGTTGT GCAAGCCAAG 
AAGTTCAGTA ATGTCACCAT GCTCTTCTCA GACATCGTTG GGTTCACTGC CATCTGCTCC 
CAGTGCTCAC CGCTGCAGGT CATCACCATG CTCAATGCAC TGTACACTCG CTTCGACCAG 
CAGTGTGGAG AGCTGGATGT CTACAAGGTG GAGACCATTG GCGATGCCTA TTGTGTAGCT 
GGGGGATTAC ACAAAGAGAG TGATACTCAT GCTGTTCAGA TAGCGCTGAT GGCCCTGAAG 
ATGATGGAGC TCTCTGATGA AGTTATGTCT CCCCATGGAG AACCTATCAA GATGCGAATT 
GGACTGCACT CTGGATCAGT TTTTGCTGGC GTCGTTGGAG TTAAAATGCC CCGTTACTGT 
CTTTTTGGAA ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA 
ATCAATGTCA GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC 
CCTCGATCAA GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT 
TTTCTGGATG CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG 
GAAGATGGCA ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263014.1
CDS	1303..2670
Translation	MPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_005262957.3
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005262957.3

ORF Insert Sequence:

ATGCCTCCCT GCTTCCATAA TGATTGCAGC GAGTTTGTGA ATCAGCCCTA CTTGTTGTAC 
TCCGTTCACA TGAAAAGCAC CAAGCCATCC CTGTCCCCCA GCAAACCCCA GTCCTCGCTG 
GTGATTCCCA CATCGCTATT CTGCAAGACA TTTCCATTCC ATTTCATGTT TGACAAAGAT 
ATGACAATTC TGCAATTTGG CAATGGCATC AGAAGGCTGA TGAACAGGAG AGACTTTCAA 
GGAAAGCCTA ATTTTGAAGA ATACTTTGAA ATTCTGACTC CAAAAATCAA CCAGACGTTT 
AGCGGGATCA TGACTATGTT GAATATGCAG TTTGTTGTAC GAGTGAGGAG ATGGGACAAC 
TCTGTGAAAA AATCTTCAAG GGTTATGGAC CTCAAAGGCC AAATGATCTA CATTGTTGAA 
TCCAGTGCAA TCTTGTTTTT GGGGTCACCC TGTGTGGACA GATTAGAAGA TTTTACAGGA 
CGAGGGCTCT ACCTCTCAGA CATCCCAATT CACAATGCAC TGAGGGATGT GGTCTTAATA 
GGGGAACAAG CCCGAGCTCA AGATGGCCTG AAGAAGAGGC TGGGGAAGCT GAAGGCTACC 
CTTGAGCAAG CCCACCAAGC CCTGGAGGAG GAGAAGAAAA AGACAGTAGA CCTTCTGTGC 
TCCATATTTC CCTGTGAGGT TGCTCAGCAG CTGTGGCAAG GGCAAGTTGT GCAAGCCAAG 
AAGTTCAGTA ATGTCACCAT GCTCTTCTCA GACATCGTTG GGTTCACTGC CATCTGCTCC 
CAGTGCTCAC CGCTGCAGGT CATCACCATG CTCAATGCAC TGTACACTCG CTTCGACCAG 
CAGTGTGGAG AGCTGGATGT CTACAAGGTG GAGACCATTG GCGATGCCTA TTGTGTAGCT 
GGGGGATTAC ACAAAGAGAG TGATACTCAT GCTGTTCAGA TAGCGCTGAT GGCCCTGAAG 
ATGATGGAGC TCTCTGATGA AGTTATGTCT CCCCATGGAG AACCTATCAA GATGCGAATT 
GGACTGCACT CTGGATCAGT TTTTGCTGGC GTCGTTGGAG TTAAAATGCC CCGTTACTGT 
CTTTTTGGAA ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA 
ATCAATGTCA GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC 
CCTCGATCAA GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT 
TTTCTGGATG CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG 
GAAGATGGCA ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of a soluble guanylate cyclase in RBCs: preserved activity in patients with coronary artery disease.
Redox biology14328-337(2018 Apr)
Cortese-Krott MM,Mergia E,Kramer CM,L?ckst?dt W,Yang J,Wolff G,Panknin C,Bracht T,Sitek B,Pernow J,Stasch JP,Feelisch M,Koesling D,Kelm M

Phenotypic Consequences of a Genetic Predisposition to Enhanced Nitric Oxide Signaling.
Circulation137(3)222-232(2018 Jan)
Emdin CA,Khera AV,Klarin D,Natarajan P,Zekavat SM,Nomura A,Haas M,Aragam K,Ardissino D,Wilson JG,Schunkert H,McPherson R,Watkins H,Elosua R,Bown MJ,Samani NJ,Baber U,Erdmann J,Gormley P,Palotie A,Stitziel NO,Gupta N,Danesh J,Saleheen D,Gabriel S,Kathiresan S

Functional Characterization of the
Circulation136(5)476-489(2017 Aug)
Kessler T,Wobst J,Wolf B,Eckhold J,Vilne B,Hollstein R,von Ameln S,Dang TA,Sager HB,Moritz Rumpf P,Aherrahrou R,Kastrati A,Bj?rkegren JLM,Erdmann J,Lusis AJ,Civelek M,Kaiser FJ,Schunkert H

Peptide B targets soluble guanylyl cyclase ?1 and kills prostate cancer cells.
PloS one12(8)e0184088(2017)
Zhou J,Gao S,Hsieh CL,Malla M,Shemshedini L

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GUCY1A1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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