gucy1a1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gucy1a1
Entrez Gene ID	550420
Full Name	guanylate cyclase 1 soluble subunit alpha 1
Synonyms	gucy1a3,im:7136149,sgc1a1,zgc:112159
General protein information	Preferred Names guanylate cyclase 1 soluble subunit alpha 1 Names guanylate cyclase soluble subunit alpha-3 guanylate cyclase 1, soluble, alpha 3 soluble guanylate cyclase large subunit alpha 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001309529.1	NP_001296458.1	guanylate cyclase soluble subunit alpha-3

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	gucy1a3	XP_004911212.1
Canis lupus familiaris (dog)	GUCY1A3	NP_001018045.1
Bos taurus (cattle)	GUCY1A3	NP_786972.1
Homo sapiens (human)	GUCY1A3	NP_001124154.1
Mus musculus (house mouse)	Gucy1a3	NP_068696.2
Rattus norvegicus (Norway rat)	Gucy1a3	NP_058786.2
Pan troglodytes (chimpanzee)	GUCY1A3	XP_003310599.1
	GUCY1A3	XP_002804280.1
Gallus gallus (chicken)	GUCY1A3	XP_420375.1
Danio rerio (zebrafish)	gucy1a3	NP_001017725.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Molecular and neuronal homology between the olfactory systems of zebrafish and mouse.
Saraiva LR, Ahuja G, Ivandic I, Syed AS, Marioni JC, Korsching SI, Logan DW
Scientific reports511487(2015 Jun)

A Zebrafish Drug-Repurposing Screen Reveals sGC-Dependent and sGC-Independent Pro-Inflammatory Activities of Nitric Oxide.
Wittmann C, Reischl M, Shah AH, Kronfuss E, Mikut R, Liebel U, Grabher C
PloS one10(10)e0137286(2015)

TET2 plays an essential role in erythropoiesis by regulating lineage-specific genes via DNA oxidative demethylation in a zebrafish model.
Ge L, Zhang RP, Wan F, Guo DY, Wang P, Xiang LX, Shao JZ
Molecular and cellular biology34(6)989-1002(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gucy1a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gucy1a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa05681	NM_001309529.1 Latest version!	Danio rerio guanylate cyclase 1 soluble subunit alpha 1 (gucy1a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gucy1a1 ( NM_001309529.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001296458.1 Danio rerio guanylate cyclase 1 soluble subunit alpha 1 (gucy1a1), mRNA.

Price & Availability↑

CloneID	ODa05681
Clone ID Related Accession (Same CDS sequence)	NM_001309529.1
Accession Version	NM_001309529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-01
Organism	Danio rerio(zebrafish)
Product	guanylate cyclase soluble subunit alpha-3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU571168.4 and HE577852.1. On Jun 10, 2015 this sequence version replaced XM_005170919.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: HE577852.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505374 [ECO:0000348] ##Evidence-Data-END##

ATGTTTTGCG CTAAACTGAA GGATCTTAAA ATTGCGGGCG AATGCCCCTT TTCTATGGTC 
TTGGGAAATG AGGAACCAGC GGACTTTGAG GGAAGCAATG CGGTGAATGT TGTGCCAGTA 
TCCAGAGAGG TGCAGGGCAA TATCACAAAC ATTCTGCCCA GACGCAAAAT AAGCCGGAGC 
AAAGTTAATC TGCATTCACT GGGAGAAAGC GTCCGAAAGC TGGTGTGTCC AGAGTTCGAG 
AAATTACGAA ATGCGTTGAT CAGAGTTATC AAACAGCAAA TAGACACGAG TTGCCCCGTA 
GCATGCAGCT CTGATGTCTC TCACACACTT GACAATGCTG AACAATTATC TGATGTCATG 
AAAAACTACT CCCTTAAAAC AGGTATCCCC ATGGACGCAC TAAAGATTTC TCTGGGTCTG 
GAGGTGTTTC GGGACTGTTA TGAAGAGGAC GGTCATATTC TCAGAGTGGT GGGGGGAGCT 
CTTCATGATT TTCTTAACAG TTTCAACGTT CTTCTGAAAC AAAGCACACC ACCCACCCCT 
GAGATACAGC GTTACATCCA CCAAGCCTCC ATCCTCTGCT TGGACAAAGA TCCAGGGCTC 
CTGACCGTCT ATTTCTTCAA TCCCCTTCCC AGCACTGAGC TCTTCTTCTC GGGTATCATC 
CAGGCGGCAG CGTGTTTGCT CTACTGCACA CGGGTGGAGG TCAGGATGGA TGTTGGGGGT 
AAAGACAGTG GCGCTCTACA GGCTGATCAT CAGCCCCATC TGCTGTACTC AGTGGTGGTG 
AGAGACGGCA GGAGCCTCAC ACCAAGCCCC ATGAGGACAC ACACTTCTGG AGACATTCCC 
CTCTCGCTGC TCTACTCCAC TTTTCCCTTC CACATTCTCC TGGATCAGGA AATGGGCCTG 
CTGCAGATCG GGGATGGTTT GAGGAGACGG CTAGGGAGAT GCAGAGATGG GCAAAGAAGA 
CCTGCTTTCA ATGAACACTT TGCCATTGTT TCACCAGAGA TACGAGCCAG CTTCCAGGAT 
ATTTTGACCA TGCTAAACAC TCAATTCTTG CTAAGGGTGA AGCAGCATGG AGCAAGCTCT 
GCTGACAGCC CGGGAAAGCA TATGGACCTA AAGGGCCAAA TGATCTTCAT GTCAGAAATG 
AGTGCTCTCC TCTTCCTCGG CTCTCCGTGT GTGGATAAGC TGGAGGAGCT GACAGGCCGG 
GGCCTTTACC TCTCGGATAT TCCCATCCAC AATGCACTAC GGGATGTTGT TCTGGTTGGG 
GAGCAAACCA AGGCCCAGGA CGGACTGAAA AAGCGTCTGG GCAAAGCCAA AGCTGCACTG 
GAACAGGCAC ACCAGGCCCT GGAGGAAGAA AAGAGGCGAA CAGTAGAGCT GCTCTTCACA 
ATCTTCCCAG GAAACGTTGC TCAGCGCTTG TGGCAGGGAC TTCCTGTGCA GGCGAAAAAG 
TTTGATCACG TCACCGTGCT GTTCTCAGAT ATAGTGGGCT TCACTGCCAT CTGCTCACGA 
TGTACACCCA TGCAGGTGGT AAATATGCTC AGTGAGCTCT ACACCAGGTT TGATCACCAC 
TGCGGTGAGC TGGATGTGTA TAAGGTGGAA ACCATCGGCG ATGCATACTG TGTAGCAGGA 
GGATTACACA AAGAGAGTCC GACCCATGCC GTCCAAATTG CTCTTATGGC TCTAAAAATG 
ATGGAGTTGT CTGATGAGGT CACGACTCCC ATGGGTGAGG TTATCAGGAT GCGTATTGGG 
ATCCATTCTG GCTCAGTTTT GGCTGGTGTG GTTGGTGTTA AAATGCCCCG CTACTGCCTT 
TTTGGCAATA ATGTCACATT AGCCAACAAG TTTGAGTCAT GTAGCCTGCC AAGAAAGATT 
AACGTCAGTC CCACGACATA CAGATTGTTA AAAGATTGTC CAGAGTTTAC TCTCATCCCC 
CGGACAAGAG AGGATCTTCC GCCCAACTTC CCCACTGACA TCCCTGGAGT TTGTTACTTC 
CTCCAGGCTC ACGATCAAAA GACAAATGCA GCTCCAGAGG AAACTCAGCC CAGTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296458.1
CDS	205..2244
Translation	MFCAKLKDLKIAGECPFSMVLGNEEPADFEGSNAVNVVPVSREVQGNITNILPRRKISRSKVNLHSLGESVRKLVCPEFEKLRNALIRVIKQQIDTSCPVACSSDVSHTLDNAEQLSDVMKNYSLKTGIPMDALKISLGLEVFRDCYEEDGHILRVVGGALHDFLNSFNVLLKQSTPPTPEIQRYIHQASILCLDKDPGLLTVYFFNPLPSTELFFSGIIQAAACLLYCTRVEVRMDVGGKDSGALQADHQPHLLYSVVVRDGRSLTPSPMRTHTSGDIPLSLLYSTFPFHILLDQEMGLLQIGDGLRRRLGRCRDGQRRPAFNEHFAIVSPEIRASFQDILTMLNTQFLLRVKQHGASSADSPGKHMDLKGQMIFMSEMSALLFLGSPCVDKLEELTGRGLYLSDIPIHNALRDVVLVGEQTKAQDGLKKRLGKAKAALEQAHQALEEEKRRTVELLFTIFPGNVAQRLWQGLPVQAKKFDHVTVLFSDIVGFTAICSRCTPMQVVNMLSELYTRFDHHCGELDVYKVETIGDAYCVAGGLHKESPTHAVQIALMALKMMELSDEVTTPMGEVIRMRIGIHSGSVLAGVVGVKMPRYCLFGNNVTLANKFESCSLPRKINVSPTTYRLLKDCPEFTLIPRTREDLPPNFPTDIPGVCYFLQAHDQKTNAAPEETQPSG

Target ORF information:

RefSeq Version	NM_001309529.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio guanylate cyclase 1 soluble subunit alpha 1 (gucy1a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001309529.1

ORF Insert Sequence:

ATGTTTTGCG CTAAACTGAA GGATCTTAAA ATTGCGGGCG AATGCCCCTT TTCTATGGTC 
TTGGGAAATG AGGAACCAGC GGACTTTGAG GGAAGCAATG CGGTGAATGT TGTGCCAGTA 
TCCAGAGAGG TGCAGGGCAA TATCACAAAC ATTCTGCCCA GACGCAAAAT AAGCCGGAGC 
AAAGTTAATC TGCATTCACT GGGAGAAAGC GTCCGAAAGC TGGTGTGTCC AGAGTTCGAG 
AAATTACGAA ATGCGTTGAT CAGAGTTATC AAACAGCAAA TAGACACGAG TTGCCCCGTA 
GCATGCAGCT CTGATGTCTC TCACACACTT GACAATGCTG AACAATTATC TGATGTCATG 
AAAAACTACT CCCTTAAAAC AGGTATCCCC ATGGACGCAC TAAAGATTTC TCTGGGTCTG 
GAGGTGTTTC GGGACTGTTA TGAAGAGGAC GGTCATATTC TCAGAGTGGT GGGGGGAGCT 
CTTCATGATT TTCTTAACAG TTTCAACGTT CTTCTGAAAC AAAGCACACC ACCCACCCCT 
GAGATACAGC GTTACATCCA CCAAGCCTCC ATCCTCTGCT TGGACAAAGA TCCAGGGCTC 
CTGACCGTCT ATTTCTTCAA TCCCCTTCCC AGCACTGAGC TCTTCTTCTC GGGTATCATC 
CAGGCGGCAG CGTGTTTGCT CTACTGCACA CGGGTGGAGG TCAGGATGGA TGTTGGGGGT 
AAAGACAGTG GCGCTCTACA GGCTGATCAT CAGCCCCATC TGCTGTACTC AGTGGTGGTG 
AGAGACGGCA GGAGCCTCAC ACCAAGCCCC ATGAGGACAC ACACTTCTGG AGACATTCCC 
CTCTCGCTGC TCTACTCCAC TTTTCCCTTC CACATTCTCC TGGATCAGGA AATGGGCCTG 
CTGCAGATCG GGGATGGTTT GAGGAGACGG CTAGGGAGAT GCAGAGATGG GCAAAGAAGA 
CCTGCTTTCA ATGAACACTT TGCCATTGTT TCACCAGAGA TACGAGCCAG CTTCCAGGAT 
ATTTTGACCA TGCTAAACAC TCAATTCTTG CTAAGGGTGA AGCAGCATGG AGCAAGCTCT 
GCTGACAGCC CGGGAAAGCA TATGGACCTA AAGGGCCAAA TGATCTTCAT GTCAGAAATG 
AGTGCTCTCC TCTTCCTCGG CTCTCCGTGT GTGGATAAGC TGGAGGAGCT GACAGGCCGG 
GGCCTTTACC TCTCGGATAT TCCCATCCAC AATGCACTAC GGGATGTTGT TCTGGTTGGG 
GAGCAAACCA AGGCCCAGGA CGGACTGAAA AAGCGTCTGG GCAAAGCCAA AGCTGCACTG 
GAACAGGCAC ACCAGGCCCT GGAGGAAGAA AAGAGGCGAA CAGTAGAGCT GCTCTTCACA 
ATCTTCCCAG GAAACGTTGC TCAGCGCTTG TGGCAGGGAC TTCCTGTGCA GGCGAAAAAG 
TTTGATCACG TCACCGTGCT GTTCTCAGAT ATAGTGGGCT TCACTGCCAT CTGCTCACGA 
TGTACACCCA TGCAGGTGGT AAATATGCTC AGTGAGCTCT ACACCAGGTT TGATCACCAC 
TGCGGTGAGC TGGATGTGTA TAAGGTGGAA ACCATCGGCG ATGCATACTG TGTAGCAGGA 
GGATTACACA AAGAGAGTCC GACCCATGCC GTCCAAATTG CTCTTATGGC TCTAAAAATG 
ATGGAGTTGT CTGATGAGGT CACGACTCCC ATGGGTGAGG TTATCAGGAT GCGTATTGGG 
ATCCATTCTG GCTCAGTTTT GGCTGGTGTG GTTGGTGTTA AAATGCCCCG CTACTGCCTT 
TTTGGCAATA ATGTCACATT AGCCAACAAG TTTGAGTCAT GTAGCCTGCC AAGAAAGATT 
AACGTCAGTC CCACGACATA CAGATTGTTA AAAGATTGTC CAGAGTTTAC TCTCATCCCC 
CGGACAAGAG AGGATCTTCC GCCCAACTTC CCCACTGACA TCCCTGGAGT TTGTTACTTC 
CTCCAGGCTC ACGATCAAAA GACAAATGCA GCTCCAGAGG AAACTCAGCC CAGTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Molecular and neuronal homology between the olfactory systems of zebrafish and mouse.
Scientific reports511487(2015 Jun)
Saraiva LR,Ahuja G,Ivandic I,Syed AS,Marioni JC,Korsching SI,Logan DW

A Zebrafish Drug-Repurposing Screen Reveals sGC-Dependent and sGC-Independent Pro-Inflammatory Activities of Nitric Oxide.
PloS one10(10)e0137286(2015)
Wittmann C,Reischl M,Shah AH,Kronfuss E,Mikut R,Liebel U,Grabher C

TET2 plays an essential role in erythropoiesis by regulating lineage-specific genes via DNA oxidative demethylation in a zebrafish model.
Molecular and cellular biology34(6)989-1002(2014 Mar)
Ge L,Zhang RP,Wan F,Guo DY,Wang P,Xiang LX,Shao JZ

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gucy1a1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

オンラインフォーム

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