SLC25A4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC25A4
Entrez Gene ID	291
Full Name	solute carrier family 25 member 4
Synonyms	AAC1,ANT,ANT 1,ANT1,MTDPS12,MTDPS12A,PEO2,PEO3,PEOA2,T1
General protein information	Preferred Names solute carrier family 25 member 4 Names ADP/ATP translocase 1 ADP,ATP carrier protein 1 ADP,ATP carrier protein, heart/skeletal muscle adenine nucleotide translocator 1 (skeletal muscle) heart/skeletal muscle ATP/ADP translocator solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q35.1
Summary	This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into the cytoplasm. The protein forms a homodimer embedded in the inner mitochondria membrane. Mutations in this gene have been shown to result in autosomal dominant progressive external opthalmoplegia and familial hypertrophic cardiomyopathy. [provided by RefSeq, Jun 2013].
Disorder MIM:	103220
Disorder Html:	Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal dominant 2, 609283 (3); Mitochondrial DNA depletion syndrome 12A (cardiomyopathic type) AD, 617184 (3); Mitochondrial DNA depletion syndrome 12B (cardiomyopathic type) AR, 615418 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001151.4	NP_001142.2	ADP/ATP translocase 1
NM_001151.3	NP_001142.2	ADP/ATP translocase 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SLC25A4	NP_777083.1
Anopheles gambiae (African malaria mosquito)	ADT2_ANOGA	XP_003436097.1
Danio rerio (zebrafish)	slc25a4	NP_999867.1
Xenopus tropicalis (tropical clawed frog)	slc25a4	NP_988909.1
	SLC25A4	XP_001084544.1
Canis lupus familiaris (dog)	SLC25A4	XP_532844.2
Mus musculus (house mouse)	Slc25a4	NP_031476.3
Gallus gallus (chicken)	SLC25A4	NP_001006443.1
Drosophila melanogaster (fruit fly)	sesB	NP_727448.1
Homo sapiens (human)	SLC25A4	NP_001142.2
Pan troglodytes (chimpanzee)	SLC25A4	XP_517556.3
Rattus norvegicus (Norway rat)	Slc25a4	NP_445967.1

Gene Ontology
Bibliography

Related articles in PubMed

Tyrosine phosphatase SHP2 negatively regulates NLRP3 inflammasome activation via ANT1-dependent mitochondrial homeostasis.
Guo W, Liu W, Chen Z, Gu Y, Peng S, Shen L, Shen Y, Wang X, Feng GS, Sun Y, Xu Q
Nature communications8(1)2168(2017 Dec)

Dominance of yeast aac2
Dallabona C, Baruffini E, Goffrini P, Lodi T
Biochemical and biophysical research communications493(2)909-913(2017 11)

Two new cases of mitochondrial myopathy with exercise intolerance, hyperlactatemia and cardiomyopathy, caused by recessive SLC25A4 mutations.
Tosserams A, Papadopoulos C, Jardel C, Lemi?re I, Romero NB, De Lonlay P, Wahbi K, Voermans N, Hogrel JY, Lafor?t P
Mitochondrion(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Yeast aac2 R96H and aac2 R252G mutations are equivalent to R80H and R235G human ANT1 pathological mutations. mtDNA instability induced by aac2R96H and aac2R252G is rescued by N-acetylcysteine.
Title: Dominance of yeast aac2

A directed proteomic approach discovered the novel interaction of BKCa with Tom22, a component of the mitochondrion outer membrane import system, and the adenine nucleotide translocator (ANT).
Title: The mitochondrial BK

identification by whole-exome sequencing of seven probands harboring dominant, de novo SLC25A4 mutations; all affected individuals presented at birth, were ventilator dependent and, where tested, revealed severe combined mitochondrial respiratory chain deficiencies associated with a marked loss of mitochondrial DNA copy number in skeletal muscle
Title: Recurrent De Novo Dominant Mutations in SLC25A4 Cause Severe Early-Onset Mitochondrial Disease and Loss of Mitochondrial DNA Copy Number.

Identification of ZNF555 as a putative transcriptional factor that impacts ANT1 promoter activity in facioscapulohumeral dystrophy myoblasts.
Title: ZNF555 protein binds to transcriptional activator site of 4qA allele and ANT1: potential implication in Facioscapulohumeral dystrophy.

Data suggest acetylation of ANT1 at lysines 10/23/92 has dramatic physiological effects on ADP-ATP exchange; extent of acetylation of lysine 23 decreases following physical activity; this change is highly dependent on insulin sensitivity/resistance.
Title: Adenine nucleotide translocase is acetylated in vivo in human muscle: Modeling predicts a decreased ADP affinity and altered control of oxidative phosphorylation.

The following SLC25A4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC25A4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22413	NM_001151.4 Latest version!	Homo sapiens solute carrier family 25 member 4 (SLC25A4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu22413	NM_001151.3	Homo sapiens solute carrier family 25 member 4 (SLC25A4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC25A4 ( NM_001151.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001142.2 Homo sapiens solute carrier family 25 member 4 (SLC25A4), mRNA.

Price & Availability↑

CloneID	OHu22413
Clone ID Related Accession (Same CDS sequence)	NM_001151.4 , NM_001151.3
Accession Version	NM_001151.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	ADP/ATP translocase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI544547.1, BC008664.1, AC093824.3, AC112237.1 and AC093592.3. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001151.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.48497.1, SRR1660805.245794.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000281456.11/ ENSP00000281456.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGTGATC ACGCTTGGAG CTTCCTAAAG GACTTCCTGG CCGGGGGCGT CGCCGCTGCC 
GTCTCCAAGA CCGCGGTCGC CCCCATCGAG AGGGTCAAAC TGCTGCTGCA GGTCCAGCAT 
GCCAGCAAAC AGATCAGTGC TGAGAAGCAG TACAAAGGGA TCATTGATTG TGTGGTGAGA 
ATCCCTAAGG AGCAGGGCTT CCTCTCCTTC TGGAGGGGTA ACCTGGCCAA CGTGATCCGT 
TACTTCCCCA CCCAAGCTCT CAACTTCGCC TTCAAGGACA AGTACAAGCA GCTCTTCTTA 
GGGGGTGTGG ATCGGCATAA GCAGTTCTGG CGCTACTTTG CTGGTAACCT GGCGTCCGGT 
GGGGCCGCTG GGGCCACCTC CCTTTGCTTT GTCTACCCGC TGGACTTTGC TAGGACCAGG 
TTGGCTGCTG ATGTGGGCAA GGGCGCCGCC CAGCGTGAGT TCCATGGTCT GGGCGACTGT 
ATCATCAAGA TCTTCAAGTC TGATGGCCTG AGGGGGCTCT ACCAGGGTTT CAACGTCTCT 
GTCCAAGGCA TCATTATCTA TAGAGCTGCC TACTTCGGAG TCTATGATAC TGCCAAGGGG 
ATGCTGCCTG ACCCCAAGAA CGTGCACATT TTTGTGAGCT GGATGATTGC CCAGAGTGTG 
ACGGCAGTCG CAGGGCTGGT GTCCTACCCC TTTGACACTG TTCGTCGTAG AATGATGATG 
CAGTCCGGCC GGAAAGGGGC CGATATTATG TACACGGGGA CAGTTGACTG CTGGAGGAAG 
ATTGCAAAAG ACGAAGGAGC CAAGGCCTTC TTCAAAGGTG CCTGGTCCAA TGTGCTGAGA 
GGCATGGGCG GTGCTTTTGT ATTGGTGTTG TATGATGAGA TCAAAAAATA TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001142.2
CDS	108..1004
Translation	MGDHAWSFLKDFLAGGVAAAVSKTAVAPIERVKLLLQVQHASKQISAEKQYKGIIDCVVRIPKEQGFLSFWRGNLANVIRYFPTQALNFAFKDKYKQLFLGGVDRHKQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKGAAQREFHGLGDCIIKIFKSDGLRGLYQGFNVSVQGIIIYRAAYFGVYDTAKGMLPDPKNVHIFVSWMIAQSVTAVAGLVSYPFDTVRRRMMMQSGRKGADIMYTGTVDCWRKIAKDEGAKAFFKGAWSNVLRGMGGAFVLVLYDEIKKYV

Target ORF information:

RefSeq Version	NM_001151.4
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 25 member 4 (SLC25A4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001151.4

ORF Insert Sequence:

ATGGGTGATC ACGCTTGGAG CTTCCTAAAG GACTTCCTGG CCGGGGGCGT CGCCGCTGCC 
GTCTCCAAGA CCGCGGTCGC CCCCATCGAG AGGGTCAAAC TGCTGCTGCA GGTCCAGCAT 
GCCAGCAAAC AGATCAGTGC TGAGAAGCAG TACAAAGGGA TCATTGATTG TGTGGTGAGA 
ATCCCTAAGG AGCAGGGCTT CCTCTCCTTC TGGAGGGGTA ACCTGGCCAA CGTGATCCGT 
TACTTCCCCA CCCAAGCTCT CAACTTCGCC TTCAAGGACA AGTACAAGCA GCTCTTCTTA 
GGGGGTGTGG ATCGGCATAA GCAGTTCTGG CGCTACTTTG CTGGTAACCT GGCGTCCGGT 
GGGGCCGCTG GGGCCACCTC CCTTTGCTTT GTCTACCCGC TGGACTTTGC TAGGACCAGG 
TTGGCTGCTG ATGTGGGCAA GGGCGCCGCC CAGCGTGAGT TCCATGGTCT GGGCGACTGT 
ATCATCAAGA TCTTCAAGTC TGATGGCCTG AGGGGGCTCT ACCAGGGTTT CAACGTCTCT 
GTCCAAGGCA TCATTATCTA TAGAGCTGCC TACTTCGGAG TCTATGATAC TGCCAAGGGG 
ATGCTGCCTG ACCCCAAGAA CGTGCACATT TTTGTGAGCT GGATGATTGC CCAGAGTGTG 
ACGGCAGTCG CAGGGCTGGT GTCCTACCCC TTTGACACTG TTCGTCGTAG AATGATGATG 
CAGTCCGGCC GGAAAGGGGC CGATATTATG TACACGGGGA CAGTTGACTG CTGGAGGAAG 
ATTGCAAAAG ACGAAGGAGC CAAGGCCTTC TTCAAAGGTG CCTGGTCCAA TGTGCTGAGA 
GGCATGGGCG GTGCTTTTGT ATTGGTGTTG TATGATGAGA TCAAAAAATA TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC25A4 ( NM_001151.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001142.2 Homo sapiens solute carrier family 25 member 4 (SLC25A4), mRNA.

Price & Availability↑

CloneID	OHu22413
Clone ID Related Accession (Same CDS sequence)	NM_001151.4 , NM_001151.3
Accession Version	NM_001151.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	ADP/ATP translocase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI544547.1, BC008664.1, AC093824.3, AC112237.1 and AC093592.3. This sequence is a reference standard in the RefSeqGene project. On Sep 15, 2009 this sequence version replaced NM_001151.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.9748.1, SRR1803613.48497.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGTGATC ACGCTTGGAG CTTCCTAAAG GACTTCCTGG CCGGGGGCGT CGCCGCTGCC 
GTCTCCAAGA CCGCGGTCGC CCCCATCGAG AGGGTCAAAC TGCTGCTGCA GGTCCAGCAT 
GCCAGCAAAC AGATCAGTGC TGAGAAGCAG TACAAAGGGA TCATTGATTG TGTGGTGAGA 
ATCCCTAAGG AGCAGGGCTT CCTCTCCTTC TGGAGGGGTA ACCTGGCCAA CGTGATCCGT 
TACTTCCCCA CCCAAGCTCT CAACTTCGCC TTCAAGGACA AGTACAAGCA GCTCTTCTTA 
GGGGGTGTGG ATCGGCATAA GCAGTTCTGG CGCTACTTTG CTGGTAACCT GGCGTCCGGT 
GGGGCCGCTG GGGCCACCTC CCTTTGCTTT GTCTACCCGC TGGACTTTGC TAGGACCAGG 
TTGGCTGCTG ATGTGGGCAA GGGCGCCGCC CAGCGTGAGT TCCATGGTCT GGGCGACTGT 
ATCATCAAGA TCTTCAAGTC TGATGGCCTG AGGGGGCTCT ACCAGGGTTT CAACGTCTCT 
GTCCAAGGCA TCATTATCTA TAGAGCTGCC TACTTCGGAG TCTATGATAC TGCCAAGGGG 
ATGCTGCCTG ACCCCAAGAA CGTGCACATT TTTGTGAGCT GGATGATTGC CCAGAGTGTG 
ACGGCAGTCG CAGGGCTGGT GTCCTACCCC TTTGACACTG TTCGTCGTAG AATGATGATG 
CAGTCCGGCC GGAAAGGGGC CGATATTATG TACACGGGGA CAGTTGACTG CTGGAGGAAG 
ATTGCAAAAG ACGAAGGAGC CAAGGCCTTC TTCAAAGGTG CCTGGTCCAA TGTGCTGAGA 
GGCATGGGCG GTGCTTTTGT ATTGGTGTTG TATGATGAGA TCAAAAAATA TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001142.2
CDS	111..1007
Translation	MGDHAWSFLKDFLAGGVAAAVSKTAVAPIERVKLLLQVQHASKQISAEKQYKGIIDCVVRIPKEQGFLSFWRGNLANVIRYFPTQALNFAFKDKYKQLFLGGVDRHKQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKGAAQREFHGLGDCIIKIFKSDGLRGLYQGFNVSVQGIIIYRAAYFGVYDTAKGMLPDPKNVHIFVSWMIAQSVTAVAGLVSYPFDTVRRRMMMQSGRKGADIMYTGTVDCWRKIAKDEGAKAFFKGAWSNVLRGMGGAFVLVLYDEIKKYV

Target ORF information:

RefSeq Version	NM_001151.3
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 25 member 4 (SLC25A4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001151.3

ORF Insert Sequence:

ATGGGTGATC ACGCTTGGAG CTTCCTAAAG GACTTCCTGG CCGGGGGCGT CGCCGCTGCC 
GTCTCCAAGA CCGCGGTCGC CCCCATCGAG AGGGTCAAAC TGCTGCTGCA GGTCCAGCAT 
GCCAGCAAAC AGATCAGTGC TGAGAAGCAG TACAAAGGGA TCATTGATTG TGTGGTGAGA 
ATCCCTAAGG AGCAGGGCTT CCTCTCCTTC TGGAGGGGTA ACCTGGCCAA CGTGATCCGT 
TACTTCCCCA CCCAAGCTCT CAACTTCGCC TTCAAGGACA AGTACAAGCA GCTCTTCTTA 
GGGGGTGTGG ATCGGCATAA GCAGTTCTGG CGCTACTTTG CTGGTAACCT GGCGTCCGGT 
GGGGCCGCTG GGGCCACCTC CCTTTGCTTT GTCTACCCGC TGGACTTTGC TAGGACCAGG 
TTGGCTGCTG ATGTGGGCAA GGGCGCCGCC CAGCGTGAGT TCCATGGTCT GGGCGACTGT 
ATCATCAAGA TCTTCAAGTC TGATGGCCTG AGGGGGCTCT ACCAGGGTTT CAACGTCTCT 
GTCCAAGGCA TCATTATCTA TAGAGCTGCC TACTTCGGAG TCTATGATAC TGCCAAGGGG 
ATGCTGCCTG ACCCCAAGAA CGTGCACATT TTTGTGAGCT GGATGATTGC CCAGAGTGTG 
ACGGCAGTCG CAGGGCTGGT GTCCTACCCC TTTGACACTG TTCGTCGTAG AATGATGATG 
CAGTCCGGCC GGAAAGGGGC CGATATTATG TACACGGGGA CAGTTGACTG CTGGAGGAAG 
ATTGCAAAAG ACGAAGGAGC CAAGGCCTTC TTCAAAGGTG CCTGGTCCAA TGTGCTGAGA 
GGCATGGGCG GTGCTTTTGT ATTGGTGTTG TATGATGAGA TCAAAAAATA TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tyrosine phosphatase SHP2 negatively regulates NLRP3 inflammasome activation via ANT1-dependent mitochondrial homeostasis.
Nature communications8(1)2168(2017 Dec)
Guo W,Liu W,Chen Z,Gu Y,Peng S,Shen L,Shen Y,Wang X,Feng GS,Sun Y,Xu Q

Dominance of yeast aac2
Biochemical and biophysical research communications493(2)909-913(2017 11)
Dallabona C,Baruffini E,Goffrini P,Lodi T

Two new cases of mitochondrial myopathy with exercise intolerance, hyperlactatemia and cardiomyopathy, caused by recessive SLC25A4 mutations.
Mitochondrion(2017 Aug)
Tosserams A,Papadopoulos C,Jardel C,Lemi?re I,Romero NB,De Lonlay P,Wahbi K,Voermans N,Hogrel JY,Lafor?t P

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SLC25A4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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