PLA2G2D cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLA2G2D
Entrez Gene ID	26279
Full Name	phospholipase A2 group IID
Synonyms	PLA2IID,SPLASH,sPLA2-IID,sPLA2S
General protein information	Preferred Names phospholipase A2 group IID Names group IID secretory phospholipase A2 GIID sPLA2 phosphatidylcholine 2-acylhydrolase 2D secretory phospholipase A2s secretory-type PLA, stroma-associated homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.12
Summary	This gene encodes a secreted member of the phospholipase A2 family, and is found in a cluster of related family members on chromosome 1. Phospholipase A2 family members hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce lysophospholipids and free fatty acid. This gene may be involved in inflammation and immune response, and in weight loss associated with chronic obstructive pulmonary disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012].
Disorder MIM:	605630

mRNA and Protein(s)

mRNA	Protein	Name
NM_012400.4	NP_036532.1	group IID secretory phospholipase A2 isoform 1 precursor
NM_012400.3	NP_036532.1	group IID secretory phospholipase A2 isoform 1 precursor
NM_001271814.1	NP_001258743.1	group IID secretory phospholipase A2 isoform 2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	PLA2G2D	NP_036532.1
	PLA2G2D	XP_001095528.1
Pan troglodytes (chimpanzee)	PLA2G2D	XP_524585.3
Rattus norvegicus (Norway rat)	Pla2g2d	NP_001013446.1
Canis lupus familiaris (dog)	PLA2G2D	XP_544524.3
Mus musculus (house mouse)	Pla2g2d	NP_035239.1

Gene Ontology
Bibliography

Related articles in PubMed

Critical role of phospholipase A2 group IID in age-related susceptibility to severe acute respiratory syndrome-CoV infection.
Vijay R, Hua X, Meyerholz DK, Miki Y, Yamamoto K, Gelb M, Murakami M, Perlman S
The Journal of experimental medicine212(11)1851-68(2015 Oct)

Assessing phospholipase A2 activity toward cardiolipin by mass spectrometry.
Hsu YH, Dumlao DS, Cao J, Dennis EA
PloS one8(3)e59267(2013)

Secretory phospholipase A2 group IID Is involved in progesterone-induced acrosomal exocytosis of human spermatozoa.
Li K, Jin JY, Chen WY, Shi QX, Ni Y, Roldan ER
Journal of andrology33(5)975-83(2012 Sep-Oct)

The bactericidal effect of human secreted group IID phospholipase A2 results from both hydrolytic and non-hydrolytic activities.
Fonseca-Maldonado R, Ferreira TL, Ward RJ
Biochimie94(6)1437-40(2012 Jun)

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
Comuzzie AG, Cole SA, Laston SL, Voruganti VS, Haack K, Gibbs RA, Butte NF
PloS one7(12)e51954(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

found to induce PLA2G2D expression in mice and in human monocyte-derived macrophages
Title: Critical role of phospholipase A2 group IID in age-related susceptibility to severe acute respiratory syndrome-CoV infection.

sPLA(2)-IID is present at the head and midpiece in the human sperm, and its activation seems to be involved in acrosomal exocytosis.
Title: Secretory phospholipase A2 group IID Is involved in progesterone-induced acrosomal exocytosis of human spermatozoa.

phospholipid bilayer permeabilization by the hsPLA2GIID is independent of catalytic activity.
Title: The bactericidal effect of human secreted group IID phospholipase A2 results from both hydrolytic and non-hydrolytic activities.

Observational study of gene-disease association. (HuGE Navigator)
Title: New genetic associations detected in a host response study to hepatitis B vaccine.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Polymorphisms in innate immunity genes and patients response to dendritic cell-based HIV immuno-treatment.

The following PLA2G2D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLA2G2D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09864	NM_001271814.1 Latest version!	Homo sapiens phospholipase A2 group IID (PLA2G2D), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu10091	NM_012400.4 Latest version!	Homo sapiens phospholipase A2 group IID (PLA2G2D), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu10091	NM_012400.3	Homo sapiens phospholipase A2 group IID (PLA2G2D), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLA2G2D ( NM_001271814.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258743.1 Homo sapiens phospholipase A2 group IID (PLA2G2D), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09864
Clone ID Related Accession (Same CDS sequence)	NM_001271814.1
Accession Version	NM_001271814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	group IID secretory phospholipase A2 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC295429.1, AK310156.1, BC025706.1 and AK026347.1. Transcript Variant: This variant (2) lacks an internal exon in the coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK310156.1, DA982802.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal (PMID:10455175) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAACTTG CACTGCTGTG TGGGCTGGTG GTGATGGCTG GTGTGATTCC AATCCAGGGC 
GGGATCCTGA ACCTGAACAA GATGGTCAAG CAAGTGACTG GGAAAATGCC CATCCTCTCC 
TACTGGCCCT ACGGCTGTCA CTGCGGACTA GGTGGCAGAG GCCAACCCAA AGATGCCACG 
GACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258743.1
CDS	69..257
Translation	MELALLCGLVVMAGVIPIQGGILNLNKMVKQVTGKMPILSYWPYGCHCGLGGRGQPKDATDC

Target ORF information:

RefSeq Version	NM_001271814.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IID (PLA2G2D), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271814.1

ORF Insert Sequence:

ATGGAACTTG CACTGCTGTG TGGGCTGGTG GTGATGGCTG GTGTGATTCC AATCCAGGGC 
GGGATCCTGA ACCTGAACAA GATGGTCAAG CAAGTGACTG GGAAAATGCC CATCCTCTCC 
TACTGGCCCT ACGGCTGTCA CTGCGGACTA GGTGGCAGAG GCCAACCCAA AGATGCCACG 
GACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLA2G2D ( NM_012400.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036532.1 Homo sapiens phospholipase A2 group IID (PLA2G2D), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10091
Clone ID Related Accession (Same CDS sequence)	NM_012400.4 , NM_012400.3
Accession Version	NM_012400.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	group IID secretory phospholipase A2 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC295429.1, BC025706.1, AK026347.1 and AL158172.5. On May 17, 2019 this sequence version replaced NM_012400.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF188625.1, DC389636.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2155628 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000375105.8/ ENSP00000364246.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAACTTG CACTGCTGTG TGGGCTGGTG GTGATGGCTG GTGTGATTCC AATCCAGGGC 
GGGATCCTGA ACCTGAACAA GATGGTCAAG CAAGTGACTG GGAAAATGCC CATCCTCTCC 
TACTGGCCCT ACGGCTGTCA CTGCGGACTA GGTGGCAGAG GCCAACCCAA AGATGCCACG 
GACTGGTGCT GCCAGACCCA TGACTGCTGC TATGACCACC TGAAGACCCA GGGGTGCAGC 
ATCTACAAGG ACTATTACAG ATACAACTTT TCCCAGGGGA ACATCCACTG CTCTGACAAG 
GGAAGCTGGT GTGAGCAGCA GCTGTGTGCC TGTGACAAGG AGGTGGCCTT CTGCCTGAAG 
CGCAACCTGG ACACCTACCA GAAGCGACTG CGTTTCTACT GGCGGCCCCA CTGCCGGGGG 
CAGACCCCTG GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036532.1
CDS	39..476
Translation	MELALLCGLVVMAGVIPIQGGILNLNKMVKQVTGKMPILSYWPYGCHCGLGGRGQPKDATDWCCQTHDCCYDHLKTQGCSIYKDYYRYNFSQGNIHCSDKGSWCEQQLCACDKEVAFCLKRNLDTYQKRLRFYWRPHCRGQTPGC

Target ORF information:

RefSeq Version	NM_012400.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IID (PLA2G2D), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012400.4

ORF Insert Sequence:

ATGGAACTTG CACTGCTGTG TGGGCTGGTG GTGATGGCTG GTGTGATTCC AATCCAGGGC 
GGGATCCTGA ACCTGAACAA GATGGTCAAG CAAGTGACTG GGAAAATGCC CATCCTCTCC 
TACTGGCCCT ACGGCTGTCA CTGCGGACTA GGTGGCAGAG GCCAACCCAA AGATGCCACG 
GACTGGTGCT GCCAGACCCA TGACTGCTGC TATGACCACC TGAAGACCCA GGGGTGCAGC 
ATCTACAAGG ACTATTACAG ATACAACTTT TCCCAGGGGA ACATCCACTG CTCTGACAAG 
GGAAGCTGGT GTGAGCAGCA GCTGTGTGCC TGTGACAAGG AGGTGGCCTT CTGCCTGAAG 
CGCAACCTGG ACACCTACCA GAAGCGACTG CGTTTCTACT GGCGGCCCCA CTGCCGGGGG 
CAGACCCCTG GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLA2G2D ( NM_012400.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036532.1 Homo sapiens phospholipase A2 group IID (PLA2G2D), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10091
Clone ID Related Accession (Same CDS sequence)	NM_012400.4 , NM_012400.3
Accession Version	NM_012400.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	group IID secretory phospholipase A2 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC295429.1, BC025706.1 and AK026347.1. On Nov 28, 2012 this sequence version replaced NM_012400.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF188625.1, AK290406.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2155628 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal (PMID:10455175) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAACTTG CACTGCTGTG TGGGCTGGTG GTGATGGCTG GTGTGATTCC AATCCAGGGC 
GGGATCCTGA ACCTGAACAA GATGGTCAAG CAAGTGACTG GGAAAATGCC CATCCTCTCC 
TACTGGCCCT ACGGCTGTCA CTGCGGACTA GGTGGCAGAG GCCAACCCAA AGATGCCACG 
GACTGGTGCT GCCAGACCCA TGACTGCTGC TATGACCACC TGAAGACCCA GGGGTGCAGC 
ATCTACAAGG ACTATTACAG ATACAACTTT TCCCAGGGGA ACATCCACTG CTCTGACAAG 
GGAAGCTGGT GTGAGCAGCA GCTGTGTGCC TGTGACAAGG AGGTGGCCTT CTGCCTGAAG 
CGCAACCTGG ACACCTACCA GAAGCGACTG CGTTTCTACT GGCGGCCCCA CTGCCGGGGG 
CAGACCCCTG GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036532.1
CDS	69..506
Translation	MELALLCGLVVMAGVIPIQGGILNLNKMVKQVTGKMPILSYWPYGCHCGLGGRGQPKDATDWCCQTHDCCYDHLKTQGCSIYKDYYRYNFSQGNIHCSDKGSWCEQQLCACDKEVAFCLKRNLDTYQKRLRFYWRPHCRGQTPGC

Target ORF information:

RefSeq Version	NM_012400.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IID (PLA2G2D), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012400.3

ORF Insert Sequence:

ATGGAACTTG CACTGCTGTG TGGGCTGGTG GTGATGGCTG GTGTGATTCC AATCCAGGGC 
GGGATCCTGA ACCTGAACAA GATGGTCAAG CAAGTGACTG GGAAAATGCC CATCCTCTCC 
TACTGGCCCT ACGGCTGTCA CTGCGGACTA GGTGGCAGAG GCCAACCCAA AGATGCCACG 
GACTGGTGCT GCCAGACCCA TGACTGCTGC TATGACCACC TGAAGACCCA GGGGTGCAGC 
ATCTACAAGG ACTATTACAG ATACAACTTT TCCCAGGGGA ACATCCACTG CTCTGACAAG 
GGAAGCTGGT GTGAGCAGCA GCTGTGTGCC TGTGACAAGG AGGTGGCCTT CTGCCTGAAG 
CGCAACCTGG ACACCTACCA GAAGCGACTG CGTTTCTACT GGCGGCCCCA CTGCCGGGGG 
CAGACCCCTG GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Critical role of phospholipase A2 group IID in age-related susceptibility to severe acute respiratory syndrome-CoV infection.
The Journal of experimental medicine212(11)1851-68(2015 Oct)
Vijay R,Hua X,Meyerholz DK,Miki Y,Yamamoto K,Gelb M,Murakami M,Perlman S

Assessing phospholipase A2 activity toward cardiolipin by mass spectrometry.
PloS one8(3)e59267(2013)
Hsu YH,Dumlao DS,Cao J,Dennis EA

Secretory phospholipase A2 group IID Is involved in progesterone-induced acrosomal exocytosis of human spermatozoa.
Journal of andrology33(5)975-83(2012 Sep-Oct)
Li K,Jin JY,Chen WY,Shi QX,Ni Y,Roldan ER

The bactericidal effect of human secreted group IID phospholipase A2 results from both hydrolytic and non-hydrolytic activities.
Biochimie94(6)1437-40(2012 Jun)
Fonseca-Maldonado R,Ferreira TL,Ward RJ

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
PloS one7(12)e51954(2012)
Comuzzie AG,Cole SA,Laston SL,Voruganti VS,Haack K,Gibbs RA,Butte NF

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PLA2G2D cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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