GEMIN5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GEMIN5
Entrez Gene ID	25929
Full Name	gem nuclear organelle associated protein 5
Synonyms	GEMIN-5
General protein information	Preferred Names gem nuclear organelle associated protein 5 Names gem-associated protein 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q33.2
Summary	This gene encodes a WD repeat protein that is a component of the survival of motor neurons (SMN) complex. The SMN complex plays a critical role in mRNA splicing through the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), and may also mediate the assembly and transport of other classes of ribonucleoproteins. The encoded protein is the snRNA-binding component of the SMN complex. Dysregulation of this gene may play a role in alternative mRNA splicing and tumor cell motility. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011].
Disorder MIM:	607005

mRNA and Protein(s)

mRNA	Protein	Name
NM_001252156.1	NP_001239085.1	gem-associated protein 5 isoform 2
NM_015465.4	NP_056280.2	gem-associated protein 5 isoform 1
NM_015465.5	NP_056280.2	gem-associated protein 5 isoform 1

Pathways from BioSystems

Homologies

	GEMIN5	XP_001112869.2
Danio rerio (zebrafish)	gemin5	XP_001339880.4
Homo sapiens (human)	GEMIN5	NP_056280.2
Xenopus tropicalis (tropical clawed frog)	gemin5	XP_004912844.1
Bos taurus (cattle)	GEMIN5	XP_003586382.1
Canis lupus familiaris (dog)	GEMIN5	XP_005619319.1
Mus musculus (house mouse)	Gemin5	NP_001160141.1
Gallus gallus (chicken)	GEMIN5	XP_414574.3
Pan troglodytes (chimpanzee)	GEMIN5	XP_527093.2
Rattus norvegicus (Norway rat)	Gemin5	NP_001165560.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Structural basis for snRNA recognition by the double-WD40 repeat domain of Gemin5.
Jin W, Wang Y, Liu CP, Yang N, Jin M, Cong Y, Wang M, Xu RM
Genes & development30(21)2391-2403(2016 Nov)

Structural insights into Gemin5-guided selection of pre-snRNAs for snRNP assembly.
Xu C, Ishikawa H, Izumikawa K, Li L, He H, Nobe Y, Yamauchi Y, Shahjee HM, Wu XH, Yu YT, Isobe T, Takahashi N, Min J
Genes & development30(21)2376-2390(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

provide mechanistic understandings of Gemin5's snRNA-binding specificity as well as valuable insights into the molecular mechanism of RNA binding by WD40 repeat proteins in general.
Title: Structural basis for snRNA recognition by the double-WD40 repeat domain of Gemin5.

the WD40 domain of Gemin5 is both necessary and sufficient for binding the Sm site of pre-snRNAs
Title: Structural insights into Gemin5-guided selection of pre-snRNAs for snRNP assembly.

These results reveal the ribosome-binding capacity of the N-ter moiety, enabling Gemin5 to control global protein synthesis.
Title: The RNA-binding protein Gemin5 binds directly to the ribosome and regulates global translation.

Gemin5 is dispensable for snRNA identification and snRNP assembly.
Title: Reconstitution of the human U snRNP assembly machinery reveals stepwise Sm protein organization.

This work both reveals a new autoregulatory pathway governing SMN expression, and identifies a new mechanism through which SMN can modulate specific mRNA expression via Gemin5.
Title: Gemin5 Binds to the Survival Motor Neuron mRNA to Regulate SMN Expression.

The following GEMIN5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GEMIN5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02477	NM_001252156.1 Latest version!	Homo sapiens gem nuclear organelle associated protein 5 (GEMIN5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu07018	NM_015465.5 Latest version!	Homo sapiens gem nuclear organelle associated protein 5 (GEMIN5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OHu07018	NM_015465.4	Homo sapiens gem nuclear organelle associated protein 5 (GEMIN5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GEMIN5 ( NM_001252156.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001239085.1 Homo sapiens gem nuclear organelle associated protein 5 (GEMIN5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02477
Clone ID Related Accession (Same CDS sequence)	NM_001252156.1
Accession Version	NM_001252156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	gem-associated protein 5 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA400244.1, BC143724.1, BC036894.1, BC014147.2, BX329222.2, AK074066.2 and AL117665.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.229292.1, SRR1660805.250702.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGG AGCCGCGGAC GCTGCCGCCC TCCCCCAACT GGTACTGCGC CCGCTGCAGC 
GATGCCGTGC CCGGGGGCCT CTTTGGCTTC GCCGCGCGGA CCTCCGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCAGGCGA GAGTCCAGGG ACACCCCCGT TTCGAGTCAT AGGAGAGTTG 
GTGGGACACA CCGAAAGGGT CTCTGGCTTC ACATTTTCTC ATCACCCTGG TCAGTACAAC 
CTCTGTGCCA CCAGCTCCGA CGATGGGACT GTGAAAATAT GGGATGTAGA GACAAAAACA 
GTTGTGACAG AACATGCACT CCATCAGCAT ACGATATCAA CATTACATTG GTCTCCTCGA 
GTAAAGGACT TAATAGTATC TGGGGATGAA AAAGGAGTAG TTTTCTGTTA CTGGTTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCCAGGACAA TTTTCTGTCT TACTTGTTCA 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT GATAATTGAC 
ATCAGTAAGA AAGGAGAAGT TATTCATAGG CTTCGAGGCC ATGATGATGA AATCCACTCC 
ATAGCCTGGT GTCCCCTGCC TGGTGAAGAT TGTTTATCTA TAAACCAAGA GGAAACTTCA 
GAAGCTGAAA TTACCAACGG GAATGCTGTA GCACAAGCTC CAGTAACAAA AGGTTGCTAC 
TTAGCCACTG GAAGCAAAGA TCAAACCATT CGAATCTGGA GCTGTTCTAG AGGCCGAGGG 
GTGATGATTT TGAAATTGCC CTTTCTGAAG AGAAGAGGAG GGGGTATAGA CCCAACTGTT 
AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAGCAATC AACCAACACA GCTGGTATCT 
AGCTGTTTTG GAGGTGAACT GTTGCAATGG GATCTCACTC AATCTTGGAG ACGGAAATAC 
ACCCTCTTCA GTGCCTCATC AGAAGGGCAA AATCATTCAA GAATTGTGTT TAATTTATGT 
CCTTTACAAA CAGAGGATGA CAAACAGCTA TTACTTTCTA CATCAATGGA TAGAGATGTA 
AAATGTTGGG ACATAGCCAC CTTGGAGTGC AGCTGGACCC TTCCTTCCCT TGGTGGGTTT 
GCATACAGCC TGGCTTTCTC TTCTGTGGAC ATAGGCTCTT TGGCCATAGG TGTTGGGGAT 
GGCATGATCC GTGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAAAATTTT 
TGGCAAGGCG TGAAGTCCAA GGTTACAGCG CTGTGCTGGC ACCCAACCAA GGAAGGTTGC 
TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTATG ACACCTACTC CAACAAGCCT 
CCACAGATTT CTAGCACATA TCATAAGAAG ACTGTATATA CTTTAGCCTG GGGGCCACCA 
GTACCCCCCA TGTCACTTGG AGGAGAAGGA GACAGACCTT CCCTTGCTTT ATACAGCTGT 
GGAGGAGAAG GGATTGTCTT ACAGCATAAT CCCTGGAAGC TTAGTGGAGA AGCCTTTGAC 
ATCAACAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG 
ATAAGTTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA 
ATATTTCAGA TTCCCAACCT GAAACTGATC TGTACTATCC AACAGCATCA CAAGCTTGTG 
AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AATTGAGCTA TCTGATGGCC 
TCTGGCTCCA ACAATGCAGT CATTTACGTG CACAACCTGA AGACTGTCAT AGAGAGCAGC 
CCTGAGTCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TCTCAGGGCA TACGGCCAAG 
ATTACCAGTG TGGCGTGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT 
GGTACAGCCC AGGTGTGGGA TGCTCTCCGG GAAGAGCCCC TGTGCAATTT CCGAGGACAT 
CGAGGTCGAC TGCTTTGTGT GGCATGGTCT CCTTTGGATC CAGACTGCAT CTATTCAGGG 
GCAGATGACT TTTGTGTGCA CAAGTGGCTC ACTTCCATGC AAGATCATTC CCGGCCTCCT 
CAAGGCAAAA AAAGTATTGA ATTGGAGAAA AAACGGCTCT CTCAACCTAA GGCAAAGCCC 
AAAAAGAAGA AAAAGCCCAC CTTGAGAACT CCTGTAAAGC TGGAATCGAT TGATGGAAAT 
GAAGAAGAAA GCATGAAGGA GAACTCAGGA CCTGTTGAGA ATGGTGTGTC AGACCAAGAA 
GGGGAGGAGC AAGCACGGGA GCCGGAATTA CCCTGTGGCC TTGCTCCAGC GGTTTCTAGA 
GAACCAGTTA TCTGCACTCC AGTTTCCTCA GGCTTTGAAA AGTCAAAAGT CACCATTAAT 
AACAAAGTCA TTTTACTGAA AAAGGAGCCA CCAAAAGAGA AGCCAGAAAC CTTAATCAAG 
AAGAGAAAAG CTCGTTCCTT GCTTCCCCTG AGTACAAGCC TGGACCACAG ATCCAAAGAG 
GAGCTTCATC AGGACTGTTT GGTACTAGCA ACTGCAAAGC ACTCCAGAGA GCTGAATGAA 
GATGTGTCTG CTGATGTTGA GGAAAGATTT CATCTGGGGC TTTTCACAGA CAGGGCTACC 
CTGTATAGAA TGATTGATAT TGAAGGAAAA GGTCACTTAG AAAATGGCCA CCCTGAGTTA 
TTTCACCAGC TTATGCTTTG GAAAGGAGAT CTCAAAGGTG TTCTCCAGAC TGCAGCAGAA 
AGAGGGGAGC TGACAGACAA CCTTGTGGCT ATGGCACCAG CAGCTGGCTA CCATGTGTGG 
CTATGGGCTG TGGAAGCTTT TGCCAAACAG CTGTGTTTTC AGGATCAGTA TGTCAAGGCT 
GCTTCTCACC TACTTTCCAT CCACAAAGTG TATGAAGCGG TGGAGCTGCT CAAGTCAAAC 
CATTTTTACA GGGAAGCTAT TGCGATTGCC AAGGCCCGGC TGCGCCCGGA GGACCCAGTC 
CTGAAGGACT TGTACCTCAG CTGGGGAACC GTCCTAGAAA GAGATGGCCA CTATGCTGTA 
GCTGCCAAAT GCTATTTAGG GGCCACTTGT GCTTATGATG CAGCCAAAGT TTTGGCCAAA 
AAGGGGGATG CGGCATCACT TAGAACGGCT GCAGAGTTGG CTGCCATCGT AGGAGAGGAT 
GAGTTGTCTG CTTCCCTGGC TCTCAGATGT GCCCAAGAGC TGCTTCTGGC CAACAACTGG 
GTGGGAGCCC AGGAAGCCCT GCAGCTGCAT GAAAGTCTAC AGGGTCAGAG ATTGGTGTTT 
TGCCTTCTGG AGCTACTGTC CAGGCATCTG GAGGAAAAGC AGCTTTCAGA GGGCAAAAGC 
TCCTCCTCTT ACCACACTTG GAACACGGGC ACCGAAGGGC CTTTCGTGGA GAGGGTGACT 
GCAGTGTGGA AGAGCATCTT CAGCCTTGAC ACCCCTGAGC AGTATCAGGA AGCCTTTCAG 
AAGCTGCAGA ACATCAAGTA CCCATCTGCT ACAAATAACA CACCTGCCAA ACAGCTCCTG 
CTTCACATTT GCCATGACTT GACCCTGGCA GTGCTGAGCC AACAGATGGC CTCCTGGGAC 
GAGGCTGTGC AGGCGCTCCT TCGGGCGGTG GTCCGGAGCT ATGACTCAGG GAGCTTCACC 
ATCATGCAGG AAGTGTACTC AGCCTTTCTC CCTGACGGCT GTGACCACCT AAGAGACAAG 
TTGGGGGACC ATCAATCCCC TGCCACACCA GCTTTCAAAA GTTTGGAGGC CTTTTTTCTT 
TATGGGCGTC TGTATGAATT CTGGTGGTCT CTCTCCAGAC CTTGCCCAAA TTCCAGTGTC 
TGGGTAAGGG CTGGTCACAG AACACTCTCT GTTGAGCCAA GCCAGCAGTT AGACACTGCC 
AGCACTGAAG AAACGGACCC TGAAACTTCT CAGCCAGAGC CAAACAGGCC TTCAGAACTA 
GACTTGAGAC TCACAGAAGA AGGTGAGCGA ATGCTGAGTA CTTTTAAGGA GCTCTTTTCA 
GAAAAGCATG CCAGTCTCCA AAACTCACAG AGAACTGTTG CTGAAGTCCA AGAGACCTTG 
GCAGAAATGA TCCGACAACA CCAAAAGAGT CAACTCTGTA AATCCACAGC AAATGGTCCT 
GATAAGAATG AACCGGAAGT AGAAGCAGAG CAGCCCCTCT GCAGTTCTCA GAGCCAGTGT 
AAAGAAGAAA AAAATGAGCC ACTTTCTCTG CCTGAGTTAA CCAAAAGGCT TACCGAGGCA 
AATCAGAGAA TGGCGAAATT TCCTGAGAGC ATTAAGGCCT GGCCCTTCCC AGATGTGCTG 
GAGTGCTGCC TCGTCCTGCT TCTCATCAGG TCCCACTTTC CTGGCTGTCT GGCCCAGGAA 
ATGCAGCAGC AGGCCCAAGA GCTCCTTCAG AAATACGGCA ACACGAAAAC TTACAGAAGA 
CACTGCCAGA CCTTCTGTAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239085.1
CDS	84..4607
Translation	MGQEPRTLPPSPNWYCARCSDAVPGGLFGFAARTSVFLVRVGPGAGESPGTPPFRVIGELVGHTERVSGFTFSHHPGQYNLCATSSDDGTVKIWDVETKTVVTEHALHQHTISTLHWSPRVKDLIVSGDEKGVVFCYWFNRNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVIHRLRGHDDEIHSIAWCPLPGEDCLSINQEETSEAEITNGNAVAQAPVTKGCYLATGSKDQTIRIWSCSRGRGVMILKLPFLKRRGGGIDPTVKERLWLTLHWPSNQPTQLVSSCFGGELLQWDLTQSWRRKYTLFSASSEGQNHSRIVFNLCPLQTEDDKQLLLSTSMDRDVKCWDIATLECSWTLPSLGGFAYSLAFSSVDIGSLAIGVGDGMIRVWNTLSIKNNYDVKNFWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYTLAWGPPVPPMSLGGEGDRPSLALYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFQIPNLKLICTIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLKTVIESSPESPVTITEPYRTLSGHTAKITSVAWSPHHDGRLVSASYDGTAQVWDALREEPLCNFRGHRGRLLCVAWSPLDPDCIYSGADDFCVHKWLTSMQDHSRPPQGKKSIELEKKRLSQPKAKPKKKKKPTLRTPVKLESIDGNEEESMKENSGPVENGVSDQEGEEQAREPELPCGLAPAVSREPVICTPVSSGFEKSKVTINNKVILLKKEPPKEKPETLIKKRKARSLLPLSTSLDHRSKEELHQDCLVLATAKHSRELNEDVSADVEERFHLGLFTDRATLYRMIDIEGKGHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPAAGYHVWLWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPVLKDLYLSWGTVLERDGHYAVAAKCYLGATCAYDAAKVLAKKGDAASLRTAAELAAIVGEDELSASLALRCAQELLLANNWVGAQEALQLHESLQGQRLVFCLLELLSRHLEEKQLSEGKSSSSYHTWNTGTEGPFVERVTAVWKSIFSLDTPEQYQEAFQKLQNIKYPSATNNTPAKQLLLHICHDLTLAVLSQQMASWDEAVQALLRAVVRSYDSGSFTIMQEVYSAFLPDGCDHLRDKLGDHQSPATPAFKSLEAFFLYGRLYEFWWSLSRPCPNSSVWVRAGHRTLSVEPSQQLDTASTEETDPETSQPEPNRPSELDLRLTEEGERMLSTFKELFSEKHASLQNSQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEVEAEQPLCSSQSQCKEEKNEPLSLPELTKRLTEANQRMAKFPESIKAWPFPDVLECCLVLLLIRSHFPGCLAQEMQQQAQELLQKYGNTKTYRRHCQTFCM

Target ORF information:

RefSeq Version	NM_001252156.1
Organism	Homo sapiens(human)
Definition	Homo sapiens gem nuclear organelle associated protein 5 (GEMIN5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252156.1

ORF Insert Sequence:

ATGGGGCAGG AGCCGCGGAC GCTGCCGCCC TCCCCCAACT GGTACTGCGC CCGCTGCAGC 
GATGCCGTGC CCGGGGGCCT CTTTGGCTTC GCCGCGCGGA CCTCCGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCAGGCGA GAGTCCAGGG ACACCCCCGT TTCGAGTCAT AGGAGAGTTG 
GTGGGACACA CCGAAAGGGT CTCTGGCTTC ACATTTTCTC ATCACCCTGG TCAGTACAAC 
CTCTGTGCCA CCAGCTCCGA CGATGGGACT GTGAAAATAT GGGATGTAGA GACAAAAACA 
GTTGTGACAG AACATGCACT CCATCAGCAT ACGATATCAA CATTACATTG GTCTCCTCGA 
GTAAAGGACT TAATAGTATC TGGGGATGAA AAAGGAGTAG TTTTCTGTTA CTGGTTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCCAGGACAA TTTTCTGTCT TACTTGTTCA 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT GATAATTGAC 
ATCAGTAAGA AAGGAGAAGT TATTCATAGG CTTCGAGGCC ATGATGATGA AATCCACTCC 
ATAGCCTGGT GTCCCCTGCC TGGTGAAGAT TGTTTATCTA TAAACCAAGA GGAAACTTCA 
GAAGCTGAAA TTACCAACGG GAATGCTGTA GCACAAGCTC CAGTAACAAA AGGTTGCTAC 
TTAGCCACTG GAAGCAAAGA TCAAACCATT CGAATCTGGA GCTGTTCTAG AGGCCGAGGG 
GTGATGATTT TGAAATTGCC CTTTCTGAAG AGAAGAGGAG GGGGTATAGA CCCAACTGTT 
AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAGCAATC AACCAACACA GCTGGTATCT 
AGCTGTTTTG GAGGTGAACT GTTGCAATGG GATCTCACTC AATCTTGGAG ACGGAAATAC 
ACCCTCTTCA GTGCCTCATC AGAAGGGCAA AATCATTCAA GAATTGTGTT TAATTTATGT 
CCTTTACAAA CAGAGGATGA CAAACAGCTA TTACTTTCTA CATCAATGGA TAGAGATGTA 
AAATGTTGGG ACATAGCCAC CTTGGAGTGC AGCTGGACCC TTCCTTCCCT TGGTGGGTTT 
GCATACAGCC TGGCTTTCTC TTCTGTGGAC ATAGGCTCTT TGGCCATAGG TGTTGGGGAT 
GGCATGATCC GTGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAAAATTTT 
TGGCAAGGCG TGAAGTCCAA GGTTACAGCG CTGTGCTGGC ACCCAACCAA GGAAGGTTGC 
TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTATG ACACCTACTC CAACAAGCCT 
CCACAGATTT CTAGCACATA TCATAAGAAG ACTGTATATA CTTTAGCCTG GGGGCCACCA 
GTACCCCCCA TGTCACTTGG AGGAGAAGGA GACAGACCTT CCCTTGCTTT ATACAGCTGT 
GGAGGAGAAG GGATTGTCTT ACAGCATAAT CCCTGGAAGC TTAGTGGAGA AGCCTTTGAC 
ATCAACAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG 
ATAAGTTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA 
ATATTTCAGA TTCCCAACCT GAAACTGATC TGTACTATCC AACAGCATCA CAAGCTTGTG 
AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AATTGAGCTA TCTGATGGCC 
TCTGGCTCCA ACAATGCAGT CATTTACGTG CACAACCTGA AGACTGTCAT AGAGAGCAGC 
CCTGAGTCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TCTCAGGGCA TACGGCCAAG 
ATTACCAGTG TGGCGTGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT 
GGTACAGCCC AGGTGTGGGA TGCTCTCCGG GAAGAGCCCC TGTGCAATTT CCGAGGACAT 
CGAGGTCGAC TGCTTTGTGT GGCATGGTCT CCTTTGGATC CAGACTGCAT CTATTCAGGG 
GCAGATGACT TTTGTGTGCA CAAGTGGCTC ACTTCCATGC AAGATCATTC CCGGCCTCCT 
CAAGGCAAAA AAAGTATTGA ATTGGAGAAA AAACGGCTCT CTCAACCTAA GGCAAAGCCC 
AAAAAGAAGA AAAAGCCCAC CTTGAGAACT CCTGTAAAGC TGGAATCGAT TGATGGAAAT 
GAAGAAGAAA GCATGAAGGA GAACTCAGGA CCTGTTGAGA ATGGTGTGTC AGACCAAGAA 
GGGGAGGAGC AAGCACGGGA GCCGGAATTA CCCTGTGGCC TTGCTCCAGC GGTTTCTAGA 
GAACCAGTTA TCTGCACTCC AGTTTCCTCA GGCTTTGAAA AGTCAAAAGT CACCATTAAT 
AACAAAGTCA TTTTACTGAA AAAGGAGCCA CCAAAAGAGA AGCCAGAAAC CTTAATCAAG 
AAGAGAAAAG CTCGTTCCTT GCTTCCCCTG AGTACAAGCC TGGACCACAG ATCCAAAGAG 
GAGCTTCATC AGGACTGTTT GGTACTAGCA ACTGCAAAGC ACTCCAGAGA GCTGAATGAA 
GATGTGTCTG CTGATGTTGA GGAAAGATTT CATCTGGGGC TTTTCACAGA CAGGGCTACC 
CTGTATAGAA TGATTGATAT TGAAGGAAAA GGTCACTTAG AAAATGGCCA CCCTGAGTTA 
TTTCACCAGC TTATGCTTTG GAAAGGAGAT CTCAAAGGTG TTCTCCAGAC TGCAGCAGAA 
AGAGGGGAGC TGACAGACAA CCTTGTGGCT ATGGCACCAG CAGCTGGCTA CCATGTGTGG 
CTATGGGCTG TGGAAGCTTT TGCCAAACAG CTGTGTTTTC AGGATCAGTA TGTCAAGGCT 
GCTTCTCACC TACTTTCCAT CCACAAAGTG TATGAAGCGG TGGAGCTGCT CAAGTCAAAC 
CATTTTTACA GGGAAGCTAT TGCGATTGCC AAGGCCCGGC TGCGCCCGGA GGACCCAGTC 
CTGAAGGACT TGTACCTCAG CTGGGGAACC GTCCTAGAAA GAGATGGCCA CTATGCTGTA 
GCTGCCAAAT GCTATTTAGG GGCCACTTGT GCTTATGATG CAGCCAAAGT TTTGGCCAAA 
AAGGGGGATG CGGCATCACT TAGAACGGCT GCAGAGTTGG CTGCCATCGT AGGAGAGGAT 
GAGTTGTCTG CTTCCCTGGC TCTCAGATGT GCCCAAGAGC TGCTTCTGGC CAACAACTGG 
GTGGGAGCCC AGGAAGCCCT GCAGCTGCAT GAAAGTCTAC AGGGTCAGAG ATTGGTGTTT 
TGCCTTCTGG AGCTACTGTC CAGGCATCTG GAGGAAAAGC AGCTTTCAGA GGGCAAAAGC 
TCCTCCTCTT ACCACACTTG GAACACGGGC ACCGAAGGGC CTTTCGTGGA GAGGGTGACT 
GCAGTGTGGA AGAGCATCTT CAGCCTTGAC ACCCCTGAGC AGTATCAGGA AGCCTTTCAG 
AAGCTGCAGA ACATCAAGTA CCCATCTGCT ACAAATAACA CACCTGCCAA ACAGCTCCTG 
CTTCACATTT GCCATGACTT GACCCTGGCA GTGCTGAGCC AACAGATGGC CTCCTGGGAC 
GAGGCTGTGC AGGCGCTCCT TCGGGCGGTG GTCCGGAGCT ATGACTCAGG GAGCTTCACC 
ATCATGCAGG AAGTGTACTC AGCCTTTCTC CCTGACGGCT GTGACCACCT AAGAGACAAG 
TTGGGGGACC ATCAATCCCC TGCCACACCA GCTTTCAAAA GTTTGGAGGC CTTTTTTCTT 
TATGGGCGTC TGTATGAATT CTGGTGGTCT CTCTCCAGAC CTTGCCCAAA TTCCAGTGTC 
TGGGTAAGGG CTGGTCACAG AACACTCTCT GTTGAGCCAA GCCAGCAGTT AGACACTGCC 
AGCACTGAAG AAACGGACCC TGAAACTTCT CAGCCAGAGC CAAACAGGCC TTCAGAACTA 
GACTTGAGAC TCACAGAAGA AGGTGAGCGA ATGCTGAGTA CTTTTAAGGA GCTCTTTTCA 
GAAAAGCATG CCAGTCTCCA AAACTCACAG AGAACTGTTG CTGAAGTCCA AGAGACCTTG 
GCAGAAATGA TCCGACAACA CCAAAAGAGT CAACTCTGTA AATCCACAGC AAATGGTCCT 
GATAAGAATG AACCGGAAGT AGAAGCAGAG CAGCCCCTCT GCAGTTCTCA GAGCCAGTGT 
AAAGAAGAAA AAAATGAGCC ACTTTCTCTG CCTGAGTTAA CCAAAAGGCT TACCGAGGCA 
AATCAGAGAA TGGCGAAATT TCCTGAGAGC ATTAAGGCCT GGCCCTTCCC AGATGTGCTG 
GAGTGCTGCC TCGTCCTGCT TCTCATCAGG TCCCACTTTC CTGGCTGTCT GGCCCAGGAA 
ATGCAGCAGC AGGCCCAAGA GCTCCTTCAG AAATACGGCA ACACGAAAAC TTACAGAAGA 
CACTGCCAGA CCTTCTGTAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN5 ( NM_015465.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056280.2 Homo sapiens gem nuclear organelle associated protein 5 (GEMIN5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu07018
Clone ID Related Accession (Same CDS sequence)	NM_015465.4 , NM_015465.5
Accession Version	NM_015465.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	gem-associated protein 5 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA400244.1, BC036894.1, BC014147.2, BX329222.2, AK074066.2 and AL117665.1. This sequence is a reference standard in the RefSeqGene project. On Nov 5, 2011 this sequence version replaced NM_015465.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK074066.2, SRR1803616.259208.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGG AGCCGCGGAC GCTGCCGCCC TCCCCCAACT GGTACTGCGC CCGCTGCAGC 
GATGCCGTGC CCGGGGGCCT CTTTGGCTTC GCCGCGCGGA CCTCCGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCAGGCGA GAGTCCAGGG ACACCCCCGT TTCGAGTCAT AGGAGAGTTG 
GTGGGACACA CCGAAAGGGT CTCTGGCTTC ACATTTTCTC ATCACCCTGG TCAGTACAAC 
CTCTGTGCCA CCAGCTCCGA CGATGGGACT GTGAAAATAT GGGATGTAGA GACAAAAACA 
GTTGTGACAG AACATGCACT CCATCAGCAT ACGATATCAA CATTACATTG GTCTCCTCGA 
GTAAAGGACT TAATAGTATC TGGGGATGAA AAAGGAGTAG TTTTCTGTTA CTGGTTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCCAGGACAA TTTTCTGTCT TACTTGTTCA 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT GATAATTGAC 
ATCAGTAAGA AAGGAGAAGT TATTCATAGG CTTCGAGGCC ATGATGATGA AATCCACTCC 
ATAGCCTGGT GTCCCCTGCC TGGTGAAGAT TGTTTATCTA TAAACCAAGA GGAAACTTCA 
GAAGAAGCTG AAATTACCAA CGGGAATGCT GTAGCACAAG CTCCAGTAAC AAAAGGTTGC 
TACTTAGCCA CTGGAAGCAA AGATCAAACC ATTCGAATCT GGAGCTGTTC TAGAGGCCGA 
GGGGTGATGA TTTTGAAATT GCCCTTTCTG AAGAGAAGAG GAGGGGGTAT AGACCCAACT 
GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAGCA ATCAACCAAC ACAGCTGGTA 
TCTAGCTGTT TTGGAGGTGA ACTGTTGCAA TGGGATCTCA CTCAATCTTG GAGACGGAAA 
TACACCCTCT TCAGTGCCTC ATCAGAAGGG CAAAATCATT CAAGAATTGT GTTTAATTTA 
TGTCCTTTAC AAACAGAGGA TGACAAACAG CTATTACTTT CTACATCAAT GGATAGAGAT 
GTAAAATGTT GGGACATAGC CACCTTGGAG TGCAGCTGGA CCCTTCCTTC CCTTGGTGGG 
TTTGCATACA GCCTGGCTTT CTCTTCTGTG GACATAGGCT CTTTGGCCAT AGGTGTTGGG 
GATGGCATGA TCCGTGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAAAAT 
TTTTGGCAAG GCGTGAAGTC CAAGGTTACA GCGCTGTGCT GGCACCCAAC CAAGGAAGGT 
TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ATGACACCTA CTCCAACAAG 
CCTCCACAGA TTTCTAGCAC ATATCATAAG AAGACTGTAT ATACTTTAGC CTGGGGGCCA 
CCAGTACCCC CCATGTCACT TGGAGGAGAA GGAGACAGAC CTTCCCTTGC TTTATACAGC 
TGTGGAGGAG AAGGGATTGT CTTACAGCAT AATCCCTGGA AGCTTAGTGG AGAAGCCTTT 
GACATCAACA AACTCATCAG GGACACCAAT TCAATCAAAT ACAAATTGCC TGTACACACA 
GAGATAAGTT GGAAAGCAGA TGGCAAAATC ATGGCTCTTG GCAATGAAGA TGGATCAATA 
GAAATATTTC AGATTCCCAA CCTGAAACTG ATCTGTACTA TCCAACAGCA TCACAAGCTT 
GTGAATACCA TTAGCTGGCA TCATGAGCAT GGCAGCCAGC CAGAATTGAG CTATCTGATG 
GCCTCTGGCT CCAACAATGC AGTCATTTAC GTGCACAACC TGAAGACTGT CATAGAGAGC 
AGCCCTGAGT CTCCAGTGAC CATTACAGAG CCCTACCGGA CCCTCTCAGG GCATACGGCC 
AAGATTACCA GTGTGGCGTG GAGCCCACAT CATGATGGAA GGCTGGTATC TGCTTCCTAT 
GATGGTACAG CCCAGGTGTG GGATGCTCTC CGGGAAGAGC CCCTGTGCAA TTTCCGAGGA 
CATCGAGGTC GACTGCTTTG TGTGGCATGG TCTCCTTTGG ATCCAGACTG CATCTATTCA 
GGGGCAGATG ACTTTTGTGT GCACAAGTGG CTCACTTCCA TGCAAGATCA TTCCCGGCCT 
CCTCAAGGCA AAAAAAGTAT TGAATTGGAG AAAAAACGGC TCTCTCAACC TAAGGCAAAG 
CCCAAAAAGA AGAAAAAGCC CACCTTGAGA ACTCCTGTAA AGCTGGAATC GATTGATGGA 
AATGAAGAAG AAAGCATGAA GGAGAACTCA GGACCTGTTG AGAATGGTGT GTCAGACCAA 
GAAGGGGAGG AGCAAGCACG GGAGCCGGAA TTACCCTGTG GCCTTGCTCC AGCGGTTTCT 
AGAGAACCAG TTATCTGCAC TCCAGTTTCC TCAGGCTTTG AAAAGTCAAA AGTCACCATT 
AATAACAAAG TCATTTTACT GAAAAAGGAG CCACCAAAAG AGAAGCCAGA AACCTTAATC 
AAGAAGAGAA AAGCTCGTTC CTTGCTTCCC CTGAGTACAA GCCTGGACCA CAGATCCAAA 
GAGGAGCTTC ATCAGGACTG TTTGGTACTA GCAACTGCAA AGCACTCCAG AGAGCTGAAT 
GAAGATGTGT CTGCTGATGT TGAGGAAAGA TTTCATCTGG GGCTTTTCAC AGACAGGGCT 
ACCCTGTATA GAATGATTGA TATTGAAGGA AAAGGTCACT TAGAAAATGG CCACCCTGAG 
TTATTTCACC AGCTTATGCT TTGGAAAGGA GATCTCAAAG GTGTTCTCCA GACTGCAGCA 
GAAAGAGGGG AGCTGACAGA CAACCTTGTG GCTATGGCAC CAGCAGCTGG CTACCATGTG 
TGGCTATGGG CTGTGGAAGC TTTTGCCAAA CAGCTGTGTT TTCAGGATCA GTATGTCAAG 
GCTGCTTCTC ACCTACTTTC CATCCACAAA GTGTATGAAG CGGTGGAGCT GCTCAAGTCA 
AACCATTTTT ACAGGGAAGC TATTGCGATT GCCAAGGCCC GGCTGCGCCC GGAGGACCCA 
GTCCTGAAGG ACTTGTACCT CAGCTGGGGA ACCGTCCTAG AAAGAGATGG CCACTATGCT 
GTAGCTGCCA AATGCTATTT AGGGGCCACT TGTGCTTATG ATGCAGCCAA AGTTTTGGCC 
AAAAAGGGGG ATGCGGCATC ACTTAGAACG GCTGCAGAGT TGGCTGCCAT CGTAGGAGAG 
GATGAGTTGT CTGCTTCCCT GGCTCTCAGA TGTGCCCAAG AGCTGCTTCT GGCCAACAAC 
TGGGTGGGAG CCCAGGAAGC CCTGCAGCTG CATGAAAGTC TACAGGGTCA GAGATTGGTG 
TTTTGCCTTC TGGAGCTACT GTCCAGGCAT CTGGAGGAAA AGCAGCTTTC AGAGGGCAAA 
AGCTCCTCCT CTTACCACAC TTGGAACACG GGCACCGAAG GGCCTTTCGT GGAGAGGGTG 
ACTGCAGTGT GGAAGAGCAT CTTCAGCCTT GACACCCCTG AGCAGTATCA GGAAGCCTTT 
CAGAAGCTGC AGAACATCAA GTACCCATCT GCTACAAATA ACACACCTGC CAAACAGCTC 
CTGCTTCACA TTTGCCATGA CTTGACCCTG GCAGTGCTGA GCCAACAGAT GGCCTCCTGG 
GACGAGGCTG TGCAGGCGCT CCTTCGGGCG GTGGTCCGGA GCTATGACTC AGGGAGCTTC 
ACCATCATGC AGGAAGTGTA CTCAGCCTTT CTCCCTGACG GCTGTGACCA CCTAAGAGAC 
AAGTTGGGGG ACCATCAATC CCCTGCCACA CCAGCTTTCA AAAGTTTGGA GGCCTTTTTT 
CTTTATGGGC GTCTGTATGA ATTCTGGTGG TCTCTCTCCA GACCTTGCCC AAATTCCAGT 
GTCTGGGTAA GGGCTGGTCA CAGAACACTC TCTGTTGAGC CAAGCCAGCA GTTAGACACT 
GCCAGCACTG AAGAAACGGA CCCTGAAACT TCTCAGCCAG AGCCAAACAG GCCTTCAGAA 
CTAGACTTGA GACTCACAGA AGAAGGTGAG CGAATGCTGA GTACTTTTAA GGAGCTCTTT 
TCAGAAAAGC ATGCCAGTCT CCAAAACTCA CAGAGAACTG TTGCTGAAGT CCAAGAGACC 
TTGGCAGAAA TGATCCGACA ACACCAAAAG AGTCAACTCT GTAAATCCAC AGCAAATGGT 
CCTGATAAGA ATGAACCGGA AGTAGAAGCA GAGCAGCCCC TCTGCAGTTC TCAGAGCCAG 
TGTAAAGAAG AAAAAAATGA GCCACTTTCT CTGCCTGAGT TAACCAAAAG GCTTACCGAG 
GCAAATCAGA GAATGGCGAA ATTTCCTGAG AGCATTAAGG CCTGGCCCTT CCCAGATGTG 
CTGGAGTGCT GCCTCGTCCT GCTTCTCATC AGGTCCCACT TTCCTGGCTG TCTGGCCCAG 
GAAATGCAGC AGCAGGCCCA AGAGCTCCTT CAGAAATACG GCAACACGAA AACTTACAGA 
AGACACTGCC AGACCTTCTG TATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056280.2
CDS	84..4610
Translation	MGQEPRTLPPSPNWYCARCSDAVPGGLFGFAARTSVFLVRVGPGAGESPGTPPFRVIGELVGHTERVSGFTFSHHPGQYNLCATSSDDGTVKIWDVETKTVVTEHALHQHTISTLHWSPRVKDLIVSGDEKGVVFCYWFNRNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVIHRLRGHDDEIHSIAWCPLPGEDCLSINQEETSEEAEITNGNAVAQAPVTKGCYLATGSKDQTIRIWSCSRGRGVMILKLPFLKRRGGGIDPTVKERLWLTLHWPSNQPTQLVSSCFGGELLQWDLTQSWRRKYTLFSASSEGQNHSRIVFNLCPLQTEDDKQLLLSTSMDRDVKCWDIATLECSWTLPSLGGFAYSLAFSSVDIGSLAIGVGDGMIRVWNTLSIKNNYDVKNFWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYTLAWGPPVPPMSLGGEGDRPSLALYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFQIPNLKLICTIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLKTVIESSPESPVTITEPYRTLSGHTAKITSVAWSPHHDGRLVSASYDGTAQVWDALREEPLCNFRGHRGRLLCVAWSPLDPDCIYSGADDFCVHKWLTSMQDHSRPPQGKKSIELEKKRLSQPKAKPKKKKKPTLRTPVKLESIDGNEEESMKENSGPVENGVSDQEGEEQAREPELPCGLAPAVSREPVICTPVSSGFEKSKVTINNKVILLKKEPPKEKPETLIKKRKARSLLPLSTSLDHRSKEELHQDCLVLATAKHSRELNEDVSADVEERFHLGLFTDRATLYRMIDIEGKGHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPAAGYHVWLWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPVLKDLYLSWGTVLERDGHYAVAAKCYLGATCAYDAAKVLAKKGDAASLRTAAELAAIVGEDELSASLALRCAQELLLANNWVGAQEALQLHESLQGQRLVFCLLELLSRHLEEKQLSEGKSSSSYHTWNTGTEGPFVERVTAVWKSIFSLDTPEQYQEAFQKLQNIKYPSATNNTPAKQLLLHICHDLTLAVLSQQMASWDEAVQALLRAVVRSYDSGSFTIMQEVYSAFLPDGCDHLRDKLGDHQSPATPAFKSLEAFFLYGRLYEFWWSLSRPCPNSSVWVRAGHRTLSVEPSQQLDTASTEETDPETSQPEPNRPSELDLRLTEEGERMLSTFKELFSEKHASLQNSQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEVEAEQPLCSSQSQCKEEKNEPLSLPELTKRLTEANQRMAKFPESIKAWPFPDVLECCLVLLLIRSHFPGCLAQEMQQQAQELLQKYGNTKTYRRHCQTFCM

Target ORF information:

RefSeq Version	NM_015465.4
Organism	Homo sapiens(human)
Definition	Homo sapiens gem nuclear organelle associated protein 5 (GEMIN5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015465.4

ORF Insert Sequence:

ATGGGGCAGG AGCCGCGGAC GCTGCCGCCC TCCCCCAACT GGTACTGCGC CCGCTGCAGC 
GATGCCGTGC CCGGGGGCCT CTTTGGCTTC GCCGCGCGGA CCTCCGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCAGGCGA GAGTCCAGGG ACACCCCCGT TTCGAGTCAT AGGAGAGTTG 
GTGGGACACA CCGAAAGGGT CTCTGGCTTC ACATTTTCTC ATCACCCTGG TCAGTACAAC 
CTCTGTGCCA CCAGCTCCGA CGATGGGACT GTGAAAATAT GGGATGTAGA GACAAAAACA 
GTTGTGACAG AACATGCACT CCATCAGCAT ACGATATCAA CATTACATTG GTCTCCTCGA 
GTAAAGGACT TAATAGTATC TGGGGATGAA AAAGGAGTAG TTTTCTGTTA CTGGTTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCCAGGACAA TTTTCTGTCT TACTTGTTCA 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT GATAATTGAC 
ATCAGTAAGA AAGGAGAAGT TATTCATAGG CTTCGAGGCC ATGATGATGA AATCCACTCC 
ATAGCCTGGT GTCCCCTGCC TGGTGAAGAT TGTTTATCTA TAAACCAAGA GGAAACTTCA 
GAAGAAGCTG AAATTACCAA CGGGAATGCT GTAGCACAAG CTCCAGTAAC AAAAGGTTGC 
TACTTAGCCA CTGGAAGCAA AGATCAAACC ATTCGAATCT GGAGCTGTTC TAGAGGCCGA 
GGGGTGATGA TTTTGAAATT GCCCTTTCTG AAGAGAAGAG GAGGGGGTAT AGACCCAACT 
GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAGCA ATCAACCAAC ACAGCTGGTA 
TCTAGCTGTT TTGGAGGTGA ACTGTTGCAA TGGGATCTCA CTCAATCTTG GAGACGGAAA 
TACACCCTCT TCAGTGCCTC ATCAGAAGGG CAAAATCATT CAAGAATTGT GTTTAATTTA 
TGTCCTTTAC AAACAGAGGA TGACAAACAG CTATTACTTT CTACATCAAT GGATAGAGAT 
GTAAAATGTT GGGACATAGC CACCTTGGAG TGCAGCTGGA CCCTTCCTTC CCTTGGTGGG 
TTTGCATACA GCCTGGCTTT CTCTTCTGTG GACATAGGCT CTTTGGCCAT AGGTGTTGGG 
GATGGCATGA TCCGTGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAAAAT 
TTTTGGCAAG GCGTGAAGTC CAAGGTTACA GCGCTGTGCT GGCACCCAAC CAAGGAAGGT 
TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ATGACACCTA CTCCAACAAG 
CCTCCACAGA TTTCTAGCAC ATATCATAAG AAGACTGTAT ATACTTTAGC CTGGGGGCCA 
CCAGTACCCC CCATGTCACT TGGAGGAGAA GGAGACAGAC CTTCCCTTGC TTTATACAGC 
TGTGGAGGAG AAGGGATTGT CTTACAGCAT AATCCCTGGA AGCTTAGTGG AGAAGCCTTT 
GACATCAACA AACTCATCAG GGACACCAAT TCAATCAAAT ACAAATTGCC TGTACACACA 
GAGATAAGTT GGAAAGCAGA TGGCAAAATC ATGGCTCTTG GCAATGAAGA TGGATCAATA 
GAAATATTTC AGATTCCCAA CCTGAAACTG ATCTGTACTA TCCAACAGCA TCACAAGCTT 
GTGAATACCA TTAGCTGGCA TCATGAGCAT GGCAGCCAGC CAGAATTGAG CTATCTGATG 
GCCTCTGGCT CCAACAATGC AGTCATTTAC GTGCACAACC TGAAGACTGT CATAGAGAGC 
AGCCCTGAGT CTCCAGTGAC CATTACAGAG CCCTACCGGA CCCTCTCAGG GCATACGGCC 
AAGATTACCA GTGTGGCGTG GAGCCCACAT CATGATGGAA GGCTGGTATC TGCTTCCTAT 
GATGGTACAG CCCAGGTGTG GGATGCTCTC CGGGAAGAGC CCCTGTGCAA TTTCCGAGGA 
CATCGAGGTC GACTGCTTTG TGTGGCATGG TCTCCTTTGG ATCCAGACTG CATCTATTCA 
GGGGCAGATG ACTTTTGTGT GCACAAGTGG CTCACTTCCA TGCAAGATCA TTCCCGGCCT 
CCTCAAGGCA AAAAAAGTAT TGAATTGGAG AAAAAACGGC TCTCTCAACC TAAGGCAAAG 
CCCAAAAAGA AGAAAAAGCC CACCTTGAGA ACTCCTGTAA AGCTGGAATC GATTGATGGA 
AATGAAGAAG AAAGCATGAA GGAGAACTCA GGACCTGTTG AGAATGGTGT GTCAGACCAA 
GAAGGGGAGG AGCAAGCACG GGAGCCGGAA TTACCCTGTG GCCTTGCTCC AGCGGTTTCT 
AGAGAACCAG TTATCTGCAC TCCAGTTTCC TCAGGCTTTG AAAAGTCAAA AGTCACCATT 
AATAACAAAG TCATTTTACT GAAAAAGGAG CCACCAAAAG AGAAGCCAGA AACCTTAATC 
AAGAAGAGAA AAGCTCGTTC CTTGCTTCCC CTGAGTACAA GCCTGGACCA CAGATCCAAA 
GAGGAGCTTC ATCAGGACTG TTTGGTACTA GCAACTGCAA AGCACTCCAG AGAGCTGAAT 
GAAGATGTGT CTGCTGATGT TGAGGAAAGA TTTCATCTGG GGCTTTTCAC AGACAGGGCT 
ACCCTGTATA GAATGATTGA TATTGAAGGA AAAGGTCACT TAGAAAATGG CCACCCTGAG 
TTATTTCACC AGCTTATGCT TTGGAAAGGA GATCTCAAAG GTGTTCTCCA GACTGCAGCA 
GAAAGAGGGG AGCTGACAGA CAACCTTGTG GCTATGGCAC CAGCAGCTGG CTACCATGTG 
TGGCTATGGG CTGTGGAAGC TTTTGCCAAA CAGCTGTGTT TTCAGGATCA GTATGTCAAG 
GCTGCTTCTC ACCTACTTTC CATCCACAAA GTGTATGAAG CGGTGGAGCT GCTCAAGTCA 
AACCATTTTT ACAGGGAAGC TATTGCGATT GCCAAGGCCC GGCTGCGCCC GGAGGACCCA 
GTCCTGAAGG ACTTGTACCT CAGCTGGGGA ACCGTCCTAG AAAGAGATGG CCACTATGCT 
GTAGCTGCCA AATGCTATTT AGGGGCCACT TGTGCTTATG ATGCAGCCAA AGTTTTGGCC 
AAAAAGGGGG ATGCGGCATC ACTTAGAACG GCTGCAGAGT TGGCTGCCAT CGTAGGAGAG 
GATGAGTTGT CTGCTTCCCT GGCTCTCAGA TGTGCCCAAG AGCTGCTTCT GGCCAACAAC 
TGGGTGGGAG CCCAGGAAGC CCTGCAGCTG CATGAAAGTC TACAGGGTCA GAGATTGGTG 
TTTTGCCTTC TGGAGCTACT GTCCAGGCAT CTGGAGGAAA AGCAGCTTTC AGAGGGCAAA 
AGCTCCTCCT CTTACCACAC TTGGAACACG GGCACCGAAG GGCCTTTCGT GGAGAGGGTG 
ACTGCAGTGT GGAAGAGCAT CTTCAGCCTT GACACCCCTG AGCAGTATCA GGAAGCCTTT 
CAGAAGCTGC AGAACATCAA GTACCCATCT GCTACAAATA ACACACCTGC CAAACAGCTC 
CTGCTTCACA TTTGCCATGA CTTGACCCTG GCAGTGCTGA GCCAACAGAT GGCCTCCTGG 
GACGAGGCTG TGCAGGCGCT CCTTCGGGCG GTGGTCCGGA GCTATGACTC AGGGAGCTTC 
ACCATCATGC AGGAAGTGTA CTCAGCCTTT CTCCCTGACG GCTGTGACCA CCTAAGAGAC 
AAGTTGGGGG ACCATCAATC CCCTGCCACA CCAGCTTTCA AAAGTTTGGA GGCCTTTTTT 
CTTTATGGGC GTCTGTATGA ATTCTGGTGG TCTCTCTCCA GACCTTGCCC AAATTCCAGT 
GTCTGGGTAA GGGCTGGTCA CAGAACACTC TCTGTTGAGC CAAGCCAGCA GTTAGACACT 
GCCAGCACTG AAGAAACGGA CCCTGAAACT TCTCAGCCAG AGCCAAACAG GCCTTCAGAA 
CTAGACTTGA GACTCACAGA AGAAGGTGAG CGAATGCTGA GTACTTTTAA GGAGCTCTTT 
TCAGAAAAGC ATGCCAGTCT CCAAAACTCA CAGAGAACTG TTGCTGAAGT CCAAGAGACC 
TTGGCAGAAA TGATCCGACA ACACCAAAAG AGTCAACTCT GTAAATCCAC AGCAAATGGT 
CCTGATAAGA ATGAACCGGA AGTAGAAGCA GAGCAGCCCC TCTGCAGTTC TCAGAGCCAG 
TGTAAAGAAG AAAAAAATGA GCCACTTTCT CTGCCTGAGT TAACCAAAAG GCTTACCGAG 
GCAAATCAGA GAATGGCGAA ATTTCCTGAG AGCATTAAGG CCTGGCCCTT CCCAGATGTG 
CTGGAGTGCT GCCTCGTCCT GCTTCTCATC AGGTCCCACT TTCCTGGCTG TCTGGCCCAG 
GAAATGCAGC AGCAGGCCCA AGAGCTCCTT CAGAAATACG GCAACACGAA AACTTACAGA 
AGACACTGCC AGACCTTCTG TATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN5 ( NM_015465.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_056280.2 Homo sapiens gem nuclear organelle associated protein 5 (GEMIN5), transcript variant 1, mRNA.

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CloneID	OHu07018
Clone ID Related Accession (Same CDS sequence)	NM_015465.4 , NM_015465.5
Accession Version	NM_015465.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	gem-associated protein 5 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA400244.1, BC036894.1, BC014147.2, BX329222.2, AK074066.2, AL117665.1 and AC008421.6. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_015465.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK074066.2, SRR1803616.259208.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000285873.8/ ENSP00000285873.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGCAGG AGCCGCGGAC GCTGCCGCCC TCCCCCAACT GGTACTGCGC CCGCTGCAGC 
GATGCCGTGC CCGGGGGCCT CTTTGGCTTC GCCGCGCGGA CCTCCGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCAGGCGA GAGTCCAGGG ACACCCCCGT TTCGAGTCAT AGGAGAGTTG 
GTGGGACACA CCGAAAGGGT CTCTGGCTTC ACATTTTCTC ATCACCCTGG TCAGTACAAC 
CTCTGTGCCA CCAGCTCCGA CGATGGGACT GTGAAAATAT GGGATGTAGA GACAAAAACA 
GTTGTGACAG AACATGCACT CCATCAGCAT ACGATATCAA CATTACATTG GTCTCCTCGA 
GTAAAGGACT TAATAGTATC TGGGGATGAA AAAGGAGTAG TTTTCTGTTA CTGGTTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCCAGGACAA TTTTCTGTCT TACTTGTTCA 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT GATAATTGAC 
ATCAGTAAGA AAGGAGAAGT TATTCATAGG CTTCGAGGCC ATGATGATGA AATCCACTCC 
ATAGCCTGGT GTCCCCTGCC TGGTGAAGAT TGTTTATCTA TAAACCAAGA GGAAACTTCA 
GAAGAAGCTG AAATTACCAA CGGGAATGCT GTAGCACAAG CTCCAGTAAC AAAAGGTTGC 
TACTTAGCCA CTGGAAGCAA AGATCAAACC ATTCGAATCT GGAGCTGTTC TAGAGGCCGA 
GGGGTGATGA TTTTGAAATT GCCCTTTCTG AAGAGAAGAG GAGGGGGTAT AGACCCAACT 
GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAGCA ATCAACCAAC ACAGCTGGTA 
TCTAGCTGTT TTGGAGGTGA ACTGTTGCAA TGGGATCTCA CTCAATCTTG GAGACGGAAA 
TACACCCTCT TCAGTGCCTC ATCAGAAGGG CAAAATCATT CAAGAATTGT GTTTAATTTA 
TGTCCTTTAC AAACAGAGGA TGACAAACAG CTATTACTTT CTACATCAAT GGATAGAGAT 
GTAAAATGTT GGGACATAGC CACCTTGGAG TGCAGCTGGA CCCTTCCTTC CCTTGGTGGG 
TTTGCATACA GCCTGGCTTT CTCTTCTGTG GACATAGGCT CTTTGGCCAT AGGTGTTGGG 
GATGGCATGA TCCGTGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAAAAT 
TTTTGGCAAG GCGTGAAGTC CAAGGTTACA GCGCTGTGCT GGCACCCAAC CAAGGAAGGT 
TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ATGACACCTA CTCCAACAAG 
CCTCCACAGA TTTCTAGCAC ATATCATAAG AAGACTGTAT ATACTTTAGC CTGGGGGCCA 
CCAGTACCCC CCATGTCACT TGGAGGAGAA GGAGACAGAC CTTCCCTTGC TTTATACAGC 
TGTGGAGGAG AAGGGATTGT CTTACAGCAT AATCCCTGGA AGCTTAGTGG AGAAGCCTTT 
GACATCAACA AACTCATCAG GGACACCAAT TCAATCAAAT ACAAATTGCC TGTACACACA 
GAGATAAGTT GGAAAGCAGA TGGCAAAATC ATGGCTCTTG GCAATGAAGA TGGATCAATA 
GAAATATTTC AGATTCCCAA CCTGAAACTG ATCTGTACTA TCCAACAGCA TCACAAGCTT 
GTGAATACCA TTAGCTGGCA TCATGAGCAT GGCAGCCAGC CAGAATTGAG CTATCTGATG 
GCCTCTGGCT CCAACAATGC AGTCATTTAC GTGCACAACC TGAAGACTGT CATAGAGAGC 
AGCCCTGAGT CTCCAGTGAC CATTACAGAG CCCTACCGGA CCCTCTCAGG GCATACGGCC 
AAGATTACCA GTGTGGCGTG GAGCCCACAT CATGATGGAA GGCTGGTATC TGCTTCCTAT 
GATGGTACAG CCCAGGTGTG GGATGCTCTC CGGGAAGAGC CCCTGTGCAA TTTCCGAGGA 
CATCGAGGTC GACTGCTTTG TGTGGCATGG TCTCCTTTGG ATCCAGACTG CATCTATTCA 
GGGGCAGATG ACTTTTGTGT GCACAAGTGG CTCACTTCCA TGCAAGATCA TTCCCGGCCT 
CCTCAAGGCA AAAAAAGTAT TGAATTGGAG AAAAAACGGC TCTCTCAACC TAAGGCAAAG 
CCCAAAAAGA AGAAAAAGCC CACCTTGAGA ACTCCTGTAA AGCTGGAATC GATTGATGGA 
AATGAAGAAG AAAGCATGAA GGAGAACTCA GGACCTGTTG AGAATGGTGT GTCAGACCAA 
GAAGGGGAGG AGCAAGCACG GGAGCCGGAA TTACCCTGTG GCCTTGCTCC AGCGGTTTCT 
AGAGAACCAG TTATCTGCAC TCCAGTTTCC TCAGGCTTTG AAAAGTCAAA AGTCACCATT 
AATAACAAAG TCATTTTACT GAAAAAGGAG CCACCAAAAG AGAAGCCAGA AACCTTAATC 
AAGAAGAGAA AAGCTCGTTC CTTGCTTCCC CTGAGTACAA GCCTGGACCA CAGATCCAAA 
GAGGAGCTTC ATCAGGACTG TTTGGTACTA GCAACTGCAA AGCACTCCAG AGAGCTGAAT 
GAAGATGTGT CTGCTGATGT TGAGGAAAGA TTTCATCTGG GGCTTTTCAC AGACAGGGCT 
ACCCTGTATA GAATGATTGA TATTGAAGGA AAAGGTCACT TAGAAAATGG CCACCCTGAG 
TTATTTCACC AGCTTATGCT TTGGAAAGGA GATCTCAAAG GTGTTCTCCA GACTGCAGCA 
GAAAGAGGGG AGCTGACAGA CAACCTTGTG GCTATGGCAC CAGCAGCTGG CTACCATGTG 
TGGCTATGGG CTGTGGAAGC TTTTGCCAAA CAGCTGTGTT TTCAGGATCA GTATGTCAAG 
GCTGCTTCTC ACCTACTTTC CATCCACAAA GTGTATGAAG CGGTGGAGCT GCTCAAGTCA 
AACCATTTTT ACAGGGAAGC TATTGCGATT GCCAAGGCCC GGCTGCGCCC GGAGGACCCA 
GTCCTGAAGG ACTTGTACCT CAGCTGGGGA ACCGTCCTAG AAAGAGATGG CCACTATGCT 
GTAGCTGCCA AATGCTATTT AGGGGCCACT TGTGCTTATG ATGCAGCCAA AGTTTTGGCC 
AAAAAGGGGG ATGCGGCATC ACTTAGAACG GCTGCAGAGT TGGCTGCCAT CGTAGGAGAG 
GATGAGTTGT CTGCTTCCCT GGCTCTCAGA TGTGCCCAAG AGCTGCTTCT GGCCAACAAC 
TGGGTGGGAG CCCAGGAAGC CCTGCAGCTG CATGAAAGTC TACAGGGTCA GAGATTGGTG 
TTTTGCCTTC TGGAGCTACT GTCCAGGCAT CTGGAGGAAA AGCAGCTTTC AGAGGGCAAA 
AGCTCCTCCT CTTACCACAC TTGGAACACG GGCACCGAAG GGCCTTTCGT GGAGAGGGTG 
ACTGCAGTGT GGAAGAGCAT CTTCAGCCTT GACACCCCTG AGCAGTATCA GGAAGCCTTT 
CAGAAGCTGC AGAACATCAA GTACCCATCT GCTACAAATA ACACACCTGC CAAACAGCTC 
CTGCTTCACA TTTGCCATGA CTTGACCCTG GCAGTGCTGA GCCAACAGAT GGCCTCCTGG 
GACGAGGCTG TGCAGGCGCT CCTTCGGGCG GTGGTCCGGA GCTATGACTC AGGGAGCTTC 
ACCATCATGC AGGAAGTGTA CTCAGCCTTT CTCCCTGACG GCTGTGACCA CCTAAGAGAC 
AAGTTGGGGG ACCATCAATC CCCTGCCACA CCAGCTTTCA AAAGTTTGGA GGCCTTTTTT 
CTTTATGGGC GTCTGTATGA ATTCTGGTGG TCTCTCTCCA GACCTTGCCC AAATTCCAGT 
GTCTGGGTAA GGGCTGGTCA CAGAACACTC TCTGTTGAGC CAAGCCAGCA GTTAGACACT 
GCCAGCACTG AAGAAACGGA CCCTGAAACT TCTCAGCCAG AGCCAAACAG GCCTTCAGAA 
CTAGACTTGA GACTCACAGA AGAAGGTGAG CGAATGCTGA GTACTTTTAA GGAGCTCTTT 
TCAGAAAAGC ATGCCAGTCT CCAAAACTCA CAGAGAACTG TTGCTGAAGT CCAAGAGACC 
TTGGCAGAAA TGATCCGACA ACACCAAAAG AGTCAACTCT GTAAATCCAC AGCAAATGGT 
CCTGATAAGA ATGAACCGGA AGTAGAAGCA GAGCAGCCCC TCTGCAGTTC TCAGAGCCAG 
TGTAAAGAAG AAAAAAATGA GCCACTTTCT CTGCCTGAGT TAACCAAAAG GCTTACCGAG 
GCAAATCAGA GAATGGCGAA ATTTCCTGAG AGCATTAAGG CCTGGCCCTT CCCAGATGTG 
CTGGAGTGCT GCCTCGTCCT GCTTCTCATC AGGTCCCACT TTCCTGGCTG TCTGGCCCAG 
GAAATGCAGC AGCAGGCCCA AGAGCTCCTT CAGAAATACG GCAACACGAA AACTTACAGA 
AGACACTGCC AGACCTTCTG TATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056280.2
CDS	79..4605
Translation	MGQEPRTLPPSPNWYCARCSDAVPGGLFGFAARTSVFLVRVGPGAGESPGTPPFRVIGELVGHTERVSGFTFSHHPGQYNLCATSSDDGTVKIWDVETKTVVTEHALHQHTISTLHWSPRVKDLIVSGDEKGVVFCYWFNRNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVIHRLRGHDDEIHSIAWCPLPGEDCLSINQEETSEEAEITNGNAVAQAPVTKGCYLATGSKDQTIRIWSCSRGRGVMILKLPFLKRRGGGIDPTVKERLWLTLHWPSNQPTQLVSSCFGGELLQWDLTQSWRRKYTLFSASSEGQNHSRIVFNLCPLQTEDDKQLLLSTSMDRDVKCWDIATLECSWTLPSLGGFAYSLAFSSVDIGSLAIGVGDGMIRVWNTLSIKNNYDVKNFWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYTLAWGPPVPPMSLGGEGDRPSLALYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFQIPNLKLICTIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLKTVIESSPESPVTITEPYRTLSGHTAKITSVAWSPHHDGRLVSASYDGTAQVWDALREEPLCNFRGHRGRLLCVAWSPLDPDCIYSGADDFCVHKWLTSMQDHSRPPQGKKSIELEKKRLSQPKAKPKKKKKPTLRTPVKLESIDGNEEESMKENSGPVENGVSDQEGEEQAREPELPCGLAPAVSREPVICTPVSSGFEKSKVTINNKVILLKKEPPKEKPETLIKKRKARSLLPLSTSLDHRSKEELHQDCLVLATAKHSRELNEDVSADVEERFHLGLFTDRATLYRMIDIEGKGHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPAAGYHVWLWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPVLKDLYLSWGTVLERDGHYAVAAKCYLGATCAYDAAKVLAKKGDAASLRTAAELAAIVGEDELSASLALRCAQELLLANNWVGAQEALQLHESLQGQRLVFCLLELLSRHLEEKQLSEGKSSSSYHTWNTGTEGPFVERVTAVWKSIFSLDTPEQYQEAFQKLQNIKYPSATNNTPAKQLLLHICHDLTLAVLSQQMASWDEAVQALLRAVVRSYDSGSFTIMQEVYSAFLPDGCDHLRDKLGDHQSPATPAFKSLEAFFLYGRLYEFWWSLSRPCPNSSVWVRAGHRTLSVEPSQQLDTASTEETDPETSQPEPNRPSELDLRLTEEGERMLSTFKELFSEKHASLQNSQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEVEAEQPLCSSQSQCKEEKNEPLSLPELTKRLTEANQRMAKFPESIKAWPFPDVLECCLVLLLIRSHFPGCLAQEMQQQAQELLQKYGNTKTYRRHCQTFCM

Target ORF information:

RefSeq Version	NM_015465.5
Organism	Homo sapiens(human)
Definition	Homo sapiens gem nuclear organelle associated protein 5 (GEMIN5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015465.5

ORF Insert Sequence:

ATGGGGCAGG AGCCGCGGAC GCTGCCGCCC TCCCCCAACT GGTACTGCGC CCGCTGCAGC 
GATGCCGTGC CCGGGGGCCT CTTTGGCTTC GCCGCGCGGA CCTCCGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCAGGCGA GAGTCCAGGG ACACCCCCGT TTCGAGTCAT AGGAGAGTTG 
GTGGGACACA CCGAAAGGGT CTCTGGCTTC ACATTTTCTC ATCACCCTGG TCAGTACAAC 
CTCTGTGCCA CCAGCTCCGA CGATGGGACT GTGAAAATAT GGGATGTAGA GACAAAAACA 
GTTGTGACAG AACATGCACT CCATCAGCAT ACGATATCAA CATTACATTG GTCTCCTCGA 
GTAAAGGACT TAATAGTATC TGGGGATGAA AAAGGAGTAG TTTTCTGTTA CTGGTTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCCAGGACAA TTTTCTGTCT TACTTGTTCA 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT GATAATTGAC 
ATCAGTAAGA AAGGAGAAGT TATTCATAGG CTTCGAGGCC ATGATGATGA AATCCACTCC 
ATAGCCTGGT GTCCCCTGCC TGGTGAAGAT TGTTTATCTA TAAACCAAGA GGAAACTTCA 
GAAGAAGCTG AAATTACCAA CGGGAATGCT GTAGCACAAG CTCCAGTAAC AAAAGGTTGC 
TACTTAGCCA CTGGAAGCAA AGATCAAACC ATTCGAATCT GGAGCTGTTC TAGAGGCCGA 
GGGGTGATGA TTTTGAAATT GCCCTTTCTG AAGAGAAGAG GAGGGGGTAT AGACCCAACT 
GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAGCA ATCAACCAAC ACAGCTGGTA 
TCTAGCTGTT TTGGAGGTGA ACTGTTGCAA TGGGATCTCA CTCAATCTTG GAGACGGAAA 
TACACCCTCT TCAGTGCCTC ATCAGAAGGG CAAAATCATT CAAGAATTGT GTTTAATTTA 
TGTCCTTTAC AAACAGAGGA TGACAAACAG CTATTACTTT CTACATCAAT GGATAGAGAT 
GTAAAATGTT GGGACATAGC CACCTTGGAG TGCAGCTGGA CCCTTCCTTC CCTTGGTGGG 
TTTGCATACA GCCTGGCTTT CTCTTCTGTG GACATAGGCT CTTTGGCCAT AGGTGTTGGG 
GATGGCATGA TCCGTGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAAAAT 
TTTTGGCAAG GCGTGAAGTC CAAGGTTACA GCGCTGTGCT GGCACCCAAC CAAGGAAGGT 
TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ATGACACCTA CTCCAACAAG 
CCTCCACAGA TTTCTAGCAC ATATCATAAG AAGACTGTAT ATACTTTAGC CTGGGGGCCA 
CCAGTACCCC CCATGTCACT TGGAGGAGAA GGAGACAGAC CTTCCCTTGC TTTATACAGC 
TGTGGAGGAG AAGGGATTGT CTTACAGCAT AATCCCTGGA AGCTTAGTGG AGAAGCCTTT 
GACATCAACA AACTCATCAG GGACACCAAT TCAATCAAAT ACAAATTGCC TGTACACACA 
GAGATAAGTT GGAAAGCAGA TGGCAAAATC ATGGCTCTTG GCAATGAAGA TGGATCAATA 
GAAATATTTC AGATTCCCAA CCTGAAACTG ATCTGTACTA TCCAACAGCA TCACAAGCTT 
GTGAATACCA TTAGCTGGCA TCATGAGCAT GGCAGCCAGC CAGAATTGAG CTATCTGATG 
GCCTCTGGCT CCAACAATGC AGTCATTTAC GTGCACAACC TGAAGACTGT CATAGAGAGC 
AGCCCTGAGT CTCCAGTGAC CATTACAGAG CCCTACCGGA CCCTCTCAGG GCATACGGCC 
AAGATTACCA GTGTGGCGTG GAGCCCACAT CATGATGGAA GGCTGGTATC TGCTTCCTAT 
GATGGTACAG CCCAGGTGTG GGATGCTCTC CGGGAAGAGC CCCTGTGCAA TTTCCGAGGA 
CATCGAGGTC GACTGCTTTG TGTGGCATGG TCTCCTTTGG ATCCAGACTG CATCTATTCA 
GGGGCAGATG ACTTTTGTGT GCACAAGTGG CTCACTTCCA TGCAAGATCA TTCCCGGCCT 
CCTCAAGGCA AAAAAAGTAT TGAATTGGAG AAAAAACGGC TCTCTCAACC TAAGGCAAAG 
CCCAAAAAGA AGAAAAAGCC CACCTTGAGA ACTCCTGTAA AGCTGGAATC GATTGATGGA 
AATGAAGAAG AAAGCATGAA GGAGAACTCA GGACCTGTTG AGAATGGTGT GTCAGACCAA 
GAAGGGGAGG AGCAAGCACG GGAGCCGGAA TTACCCTGTG GCCTTGCTCC AGCGGTTTCT 
AGAGAACCAG TTATCTGCAC TCCAGTTTCC TCAGGCTTTG AAAAGTCAAA AGTCACCATT 
AATAACAAAG TCATTTTACT GAAAAAGGAG CCACCAAAAG AGAAGCCAGA AACCTTAATC 
AAGAAGAGAA AAGCTCGTTC CTTGCTTCCC CTGAGTACAA GCCTGGACCA CAGATCCAAA 
GAGGAGCTTC ATCAGGACTG TTTGGTACTA GCAACTGCAA AGCACTCCAG AGAGCTGAAT 
GAAGATGTGT CTGCTGATGT TGAGGAAAGA TTTCATCTGG GGCTTTTCAC AGACAGGGCT 
ACCCTGTATA GAATGATTGA TATTGAAGGA AAAGGTCACT TAGAAAATGG CCACCCTGAG 
TTATTTCACC AGCTTATGCT TTGGAAAGGA GATCTCAAAG GTGTTCTCCA GACTGCAGCA 
GAAAGAGGGG AGCTGACAGA CAACCTTGTG GCTATGGCAC CAGCAGCTGG CTACCATGTG 
TGGCTATGGG CTGTGGAAGC TTTTGCCAAA CAGCTGTGTT TTCAGGATCA GTATGTCAAG 
GCTGCTTCTC ACCTACTTTC CATCCACAAA GTGTATGAAG CGGTGGAGCT GCTCAAGTCA 
AACCATTTTT ACAGGGAAGC TATTGCGATT GCCAAGGCCC GGCTGCGCCC GGAGGACCCA 
GTCCTGAAGG ACTTGTACCT CAGCTGGGGA ACCGTCCTAG AAAGAGATGG CCACTATGCT 
GTAGCTGCCA AATGCTATTT AGGGGCCACT TGTGCTTATG ATGCAGCCAA AGTTTTGGCC 
AAAAAGGGGG ATGCGGCATC ACTTAGAACG GCTGCAGAGT TGGCTGCCAT CGTAGGAGAG 
GATGAGTTGT CTGCTTCCCT GGCTCTCAGA TGTGCCCAAG AGCTGCTTCT GGCCAACAAC 
TGGGTGGGAG CCCAGGAAGC CCTGCAGCTG CATGAAAGTC TACAGGGTCA GAGATTGGTG 
TTTTGCCTTC TGGAGCTACT GTCCAGGCAT CTGGAGGAAA AGCAGCTTTC AGAGGGCAAA 
AGCTCCTCCT CTTACCACAC TTGGAACACG GGCACCGAAG GGCCTTTCGT GGAGAGGGTG 
ACTGCAGTGT GGAAGAGCAT CTTCAGCCTT GACACCCCTG AGCAGTATCA GGAAGCCTTT 
CAGAAGCTGC AGAACATCAA GTACCCATCT GCTACAAATA ACACACCTGC CAAACAGCTC 
CTGCTTCACA TTTGCCATGA CTTGACCCTG GCAGTGCTGA GCCAACAGAT GGCCTCCTGG 
GACGAGGCTG TGCAGGCGCT CCTTCGGGCG GTGGTCCGGA GCTATGACTC AGGGAGCTTC 
ACCATCATGC AGGAAGTGTA CTCAGCCTTT CTCCCTGACG GCTGTGACCA CCTAAGAGAC 
AAGTTGGGGG ACCATCAATC CCCTGCCACA CCAGCTTTCA AAAGTTTGGA GGCCTTTTTT 
CTTTATGGGC GTCTGTATGA ATTCTGGTGG TCTCTCTCCA GACCTTGCCC AAATTCCAGT 
GTCTGGGTAA GGGCTGGTCA CAGAACACTC TCTGTTGAGC CAAGCCAGCA GTTAGACACT 
GCCAGCACTG AAGAAACGGA CCCTGAAACT TCTCAGCCAG AGCCAAACAG GCCTTCAGAA 
CTAGACTTGA GACTCACAGA AGAAGGTGAG CGAATGCTGA GTACTTTTAA GGAGCTCTTT 
TCAGAAAAGC ATGCCAGTCT CCAAAACTCA CAGAGAACTG TTGCTGAAGT CCAAGAGACC 
TTGGCAGAAA TGATCCGACA ACACCAAAAG AGTCAACTCT GTAAATCCAC AGCAAATGGT 
CCTGATAAGA ATGAACCGGA AGTAGAAGCA GAGCAGCCCC TCTGCAGTTC TCAGAGCCAG 
TGTAAAGAAG AAAAAAATGA GCCACTTTCT CTGCCTGAGT TAACCAAAAG GCTTACCGAG 
GCAAATCAGA GAATGGCGAA ATTTCCTGAG AGCATTAAGG CCTGGCCCTT CCCAGATGTG 
CTGGAGTGCT GCCTCGTCCT GCTTCTCATC AGGTCCCACT TTCCTGGCTG TCTGGCCCAG 
GAAATGCAGC AGCAGGCCCA AGAGCTCCTT CAGAAATACG GCAACACGAA AACTTACAGA 
AGACACTGCC AGACCTTCTG TATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Structural basis for snRNA recognition by the double-WD40 repeat domain of Gemin5.
Genes & development30(21)2391-2403(2016 Nov)
Jin W,Wang Y,Liu CP,Yang N,Jin M,Cong Y,Wang M,Xu RM

Structural insights into Gemin5-guided selection of pre-snRNAs for snRNP assembly.
Genes & development30(21)2376-2390(2016 Nov)
Xu C,Ishikawa H,Izumikawa K,Li L,He H,Nobe Y,Yamauchi Y,Shahjee HM,Wu XH,Yu YT,Isobe T,Takahashi N,Min J

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GEMIN5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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