GEMIN5 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010807535.2	XP_010805837.1	gem-associated protein 5 isoform X1
XM_010807537.3	XP_010805839.2	gem-associated protein 5 isoform X3
XM_010798518.2	XP_010796820.2	gem-associated protein 5 isoform X1
XM_015464342.1	XP_015319828.1	gem-associated protein 5 isoform X4
XM_010807535.3	XP_010805837.2	gem-associated protein 5 isoform X1
XM_024995269.1	XP_024851037.1	gem-associated protein 5 isoform X4
XM_010807538.2	XP_010805840.1	gem-associated protein 5 isoform X4
XM_010798519.2	XP_010796821.2	gem-associated protein 5 isoform X2
XM_010807536.3	XP_010805838.2	gem-associated protein 5 isoform X2
XM_010807538.3	XP_010805840.2	gem-associated protein 5 isoform X5
XM_015464341.1	XP_015319827.1	gem-associated protein 5 isoform X3
XM_010807536.2	XP_010805838.1	gem-associated protein 5 isoform X2
XM_010807537.2	XP_010805839.1	gem-associated protein 5 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa236777	XM_024995269.1 Latest version!	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa236774	XM_010807535.3 Latest version!	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa236775	XM_010807536.3 Latest version!	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa236776	XM_010807537.3 Latest version!	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa236778	XM_010807538.3 Latest version!	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa52456	XM_010798518.2 Latest version!	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa52456	XM_010807535.2 Latest version!	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa52457	XM_010798519.2 Latest version!	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa52457	XM_010807536.2 Latest version!	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa52458	XM_015464341.1 Latest version!	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa52458	XM_010807537.2 Latest version!	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa52459	XM_015464342.1 Latest version!	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa52459	XM_010807538.2 Latest version!	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

GEMIN5 ( XM_024995269.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851037.1 Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa236777
Clone ID Related Accession (Same CDS sequence)	XM_024995269.1
Accession Version	XM_024995269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	gem-associated protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAATG GGAAAGTTAT AACACAAACT CCAGTAACAA AAGGCTGCTA CTTAGCCACT 
GGGAGCAAAG ACCAGACCAT TCGGATCTGG AGCTGCTCTA GAGGCCGAGG GGTGATGACT 
TTGAAACTGC CCTTTCTTAA AAGAAGAGGA GGGGGTGTAG ACCCAACTGT TAAAGAGCGC 
CTTTGGTTGA CACTCCATTG GCCCAAGGAT CAGCCAACTC AGCTGGTATC CAGCTGTTTT 
GGAGGTGAAC TGCTGCTATG GGATCTCACT CAGTCTTGGA GACGGAAATA TACCCTCTTT 
AGTGGTTCAT CAGAAGGGCA AAATCATTCA AGGATTGTGT TTAATTTATG TCCTCTGCAA 
ACTGAAGATG ACAAACAGCT GCTGCTTTCC ATATCAATGG ACAGAGATGT AAAATGTTGG 
GACATGGCCA CTCTGGAGTG CTGCTGGAGC CTGCCTTCCC TCGGTGGGTT TGCCTACAGT 
CTGGCTTTCT CTCCTGTGGA CACAGGCTGC TTAGCGATAG GCGTCGGGGA TGGCATGATC 
CGCGTATGGA ATACACTCTC CATAAAGAAC AACTATGATG TGAAACATTT TTGGCAAGGT 
GTCAAGTCCA AGGTTACAGC GCTGTGCTGG CACCCGACCA AGGAAGGCTG CTTAGCTTTT 
GGAACTGATG ATGGAAAAGT GGGATTGTAC GACACCTACT CCAACAAACC TCCACAGATT 
TCCAGCACGT ATCACAAGAA GACTGTGTAC TCGTTAGCCT GGGGACCACC AGTACCCGCC 
ATGGCACTAG GAGAAGGAGA CAGACCTTCC CTCACTTTAT ACAGTTGTGG AGGAGAAGGG 
ATTGTCTTAC AGCACAACCC TTGGAAGCTT AGTGGAGAGG CCTTTGACAT CAATAAACTC 
ATCAGGGACA CCAATTCAAT CAAATACAAA TTGCCTGTAC ACACAGAGAT CAGCTGGAAA 
GCAGATGGCA AAATCATGGC TCTTGGCAAT GAAGATGGAT CAATAGAAAT ATTCAAGCTT 
CCTAACTTGA AACTGATCTG CAGCATCCAG CAGCATCATA AGCTTGTGAA TACCATTAGC 
TGGCATCATG AGCATGGCAG CCAGCCAGAA CTGAGCTATC TGATGGCCTC TGGATCCAAC 
AATGCAGTCA TTTATGTGCA CAACCTGCAG ACTGTCATAG AGAGCAATCC TCAATCTCCA 
GTGACCATTA CAGAGCCCTA CCGGACCCTG TCAGGGCACA CAGCCAAGAT CACCAGTCTG 
GCATGGAGCC CACATCATGA TGGAAGGCTG GTATCTGCTT CCTATGATGG TACAGCTCAG 
GTGTGGGATA CTCTCCAGGA AGAGCCTCTG TGCAATTTCC GGGGACATCG AGGTCGACTG 
CTTTGCGTTG TGTGGTCCCC GTTGGATCCA GATTGCATCT ACTCAGGGGC AGATGATTTC 
TGTGTGTATA AATGGCTCAC TTCCATGCAG GAACACTCCC GACCTCCTCA AGGCAAGAAA 
AGTATTGAAT TAGAGAAAAA GCGGCTCTCT CAACCTAAAC CAAAGCCCAA AAAGAAGAAA 
AAGCCCACCT TGAGAGTGCC TGTAAGGCAA GAATTATGTG ATGGGAATGA AGAAGAGAGT 
GTAAAGGAGA GCCTGGGCCC TATTGAAAAT GGCGTGTCAG ACCAGGAGAC TGAGGAGGAA 
GCCCGTGAGC CAGAGCCACC CTGTGGTGTC ACCGCAGCGG TTTCTAAAGA ACCAATTATC 
TGCACTCCAG CAGCCTTAGG TTTTGAAAAG TCAAAAAGCA CCATTAATAA CAAAGTCACT 
TTACTGAAAA AGGAGGTACC TAAAGAGAAG CCAGAAGCCT TAATCAAGAA GCGAAAAGCT 
CGTTCCATGC TTCCACTGAG TACAAGTCTG GACCACAGAT CCAAAGAGGA GCTTCATCAG 
GACTGTTTGG TATTAGCCAC CGCCAAACAC TCCACAGCAG GGCCAAACGA GGATGTGTCT 
ACTGATCTTG AGGAAAGATT TCACCTGGGG CTTTTCACAG ACAGGGCTAC CCTGTACAGA 
ATGATTGAGG TTGAAGGAAA AAGTCACTTG GAAAATGGCC ACCCCGAGTT ATTTCACCAG 
CTCATGCTTT GGAAAGGAGA TCTAAAAGGT GTTCTGCAGA CTGCAGCAGA AAGAGGGGAG 
CTGACAGACA ATCTCGTGGC TATGGCACCA GTCGCCGGTT ACCATGTGTG GGTATGGGCT 
GTAGAAGCTT TTGCCAAACA ACTGTGTTTT CAGGACCAGT ATGTCAAGGC TGCCTCTCAC 
CTGCTTTCTA TCCACAAAGT GTATGAAGCT GTGGAGCTGC TCAAGTCAAA CCATTTCTAC 
AGGGAAGCTA TTGCAATTGC TAAGGCCCGG CTGCGCCCAG AGGACCCAAT CATAAAAGAC 
CTGTACCTCA GCTGGGGAGC CATCCTAGAA AAAGACGGCC ACTATGCCTT AGCAGCAAAA 
TGCTATTTAG GGGCCACTTC AGCTTATGAT GCAGCCAAAG TTCTGGCCAA AAAGGGGGAT 
GCGGCATCAC TTAGGACGGC AGCAGAGTTG GCCTCCATCG TGGGAGAGAA TGAGTTGTCA 
GCTTCCCTGG CTCTAAGATG TGCCCAAGAA CTGCTTCTGG CCCGGAACTG GGTGGGAGCT 
CAGGAGGCCC TGCAGCTCCA CACAAGTCTA AAGGGTCAGA GATTGGTGTT TTGCCTTCTT 
GAGCTACTGT CCAAGCACCT GGAGGAAAAG CAGCTCTCTG AGGGCAAAAG CTCCTTTTCT 
TCTTACAATG ATTGGCCCAC AGACAGCGAA GGGTCCTTTG TGGAGAGGGT GACTGCAGTA 
TGGAAGAGCA CCTTCGGCCT CGACTCCCCA GAGCAGTACC AGGCAGCATT TCAGCAGCTG 
CAAAATATCA AGTACCCATC TGCTACAAGT AACACTCCCT CCAAACAGCT TCTGCTCCAC 
ATTTGCCACG ACCTGACCTT GGCAGTGCTA AGCCAGGAAG CGGGCTCCTG GGACGCAGCT 
GTGGAAGGAT TGCTTCGGGC TGTGATCCGG AGCTACGACT CAGCAAACTT CACCATCATG 
CAGGAAGTGC ACTCAGCCTT TCTTCCGCAG GGCTGTGACC ATCTAAGAGA CAAACTGGGT 
GATCATCAGT CTCCCACCAC ACCAGCTTTC AAAAGTCTGA AGGCCTTTTT TATTTACGGA 
TGTCTATATG AATTCTGGTG GTCTCTCTCT GGGCCTTGCC CGGAGTCCAG TGTCTGGCTA 
AGAGCTGCTC ACAGAAGTAA AACCTCTACT GAACAGAGCC AAGAGGTTGA CAGTGTCAGC 
CCTGAAGAAA CTGACCCCAA ACCTTCCCAG CCAGAGCCAA GCAGGACTCC AGAACTAGAC 
GTGAGACTTT CAGAAGAAGG TGAACAAATG CAGAACGCTT GTAAGGAGCT TTTGTCAGAA 
AAGCATGCTG GTCTCCAAAA TGCACAGAGA ACTGTTGCTG AAGTCCAAGA GACCTTGGCA 
GAAATGATCC GCCAACACCA GAAGAGTCAA CTGTGTAAAT CCACAGCAAA TGGCCCTGAT 
AAGAATGAAC CTGAACAGGA AGGACAGTCC CTCTCTCATC CTCAGAACCA GTGTAAAGAA 
GAAAAAAATG AACCAGTTTC TTTGCCTGAA TTAACCAAAA GACTTACAGA GGCAAATGAG 
AGAATAGCTA AGCTTCCTGA GAGTATTAAG ACCTGGCCTT TCCCGGACGT GCTGGAATGC 
TGCCTCATCC TGCTTCACAT TGGGTCCCAA TGTCCTAGCT TTGTGACCCA GGAAATGCAG 
CAACAGGCTC AAGAGTTCCT TCAGAAGTAC GGCAACACAA AAGTTTACAA ACGACATTAC 
CAGACCCTCT GCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851037.1
CDS	145..4002
Translation	MPNGKVITQTPVTKGCYLATGSKDQTIRIWSCSRGRGVMTLKLPFLKRRGGGVDPTVKERLWLTLHWPKDQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSGSSEGQNHSRIVFNLCPLQTEDDKQLLLSISMDRDVKCWDMATLECCWSLPSLGGFAYSLAFSPVDTGCLAIGVGDGMIRVWNTLSIKNNYDVKHFWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYSLAWGPPVPAMALGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFKLPNLKLICSIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLQTVIESNPQSPVTITEPYRTLSGHTAKITSLAWSPHHDGRLVSASYDGTAQVWDTLQEEPLCNFRGHRGRLLCVVWSPLDPDCIYSGADDFCVYKWLTSMQEHSRPPQGKKSIELEKKRLSQPKPKPKKKKKPTLRVPVRQELCDGNEEESVKESLGPIENGVSDQETEEEAREPEPPCGVTAAVSKEPIICTPAALGFEKSKSTINNKVTLLKKEVPKEKPEALIKKRKARSMLPLSTSLDHRSKEELHQDCLVLATAKHSTAGPNEDVSTDLEERFHLGLFTDRATLYRMIEVEGKSHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPVAGYHVWVWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPIIKDLYLSWGAILEKDGHYALAAKCYLGATSAYDAAKVLAKKGDAASLRTAAELASIVGENELSASLALRCAQELLLARNWVGAQEALQLHTSLKGQRLVFCLLELLSKHLEEKQLSEGKSSFSSYNDWPTDSEGSFVERVTAVWKSTFGLDSPEQYQAAFQQLQNIKYPSATSNTPSKQLLLHICHDLTLAVLSQEAGSWDAAVEGLLRAVIRSYDSANFTIMQEVHSAFLPQGCDHLRDKLGDHQSPTTPAFKSLKAFFIYGCLYEFWWSLSGPCPESSVWLRAAHRSKTSTEQSQEVDSVSPEETDPKPSQPEPSRTPELDVRLSEEGEQMQNACKELLSEKHAGLQNAQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEQEGQSLSHPQNQCKEEKNEPVSLPELTKRLTEANERIAKLPESIKTWPFPDVLECCLILLHIGSQCPSFVTQEMQQQAQEFLQKYGNTKVYKRHYQTLCT

Target ORF information:

RefSeq Version	XM_024995269.1
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995269.1

ORF Insert Sequence:

ATGCCCAATG GGAAAGTTAT AACACAAACT CCAGTAACAA AAGGCTGCTA CTTAGCCACT 
GGGAGCAAAG ACCAGACCAT TCGGATCTGG AGCTGCTCTA GAGGCCGAGG GGTGATGACT 
TTGAAACTGC CCTTTCTTAA AAGAAGAGGA GGGGGTGTAG ACCCAACTGT TAAAGAGCGC 
CTTTGGTTGA CACTCCATTG GCCCAAGGAT CAGCCAACTC AGCTGGTATC CAGCTGTTTT 
GGAGGTGAAC TGCTGCTATG GGATCTCACT CAGTCTTGGA GACGGAAATA TACCCTCTTT 
AGTGGTTCAT CAGAAGGGCA AAATCATTCA AGGATTGTGT TTAATTTATG TCCTCTGCAA 
ACTGAAGATG ACAAACAGCT GCTGCTTTCC ATATCAATGG ACAGAGATGT AAAATGTTGG 
GACATGGCCA CTCTGGAGTG CTGCTGGAGC CTGCCTTCCC TCGGTGGGTT TGCCTACAGT 
CTGGCTTTCT CTCCTGTGGA CACAGGCTGC TTAGCGATAG GCGTCGGGGA TGGCATGATC 
CGCGTATGGA ATACACTCTC CATAAAGAAC AACTATGATG TGAAACATTT TTGGCAAGGT 
GTCAAGTCCA AGGTTACAGC GCTGTGCTGG CACCCGACCA AGGAAGGCTG CTTAGCTTTT 
GGAACTGATG ATGGAAAAGT GGGATTGTAC GACACCTACT CCAACAAACC TCCACAGATT 
TCCAGCACGT ATCACAAGAA GACTGTGTAC TCGTTAGCCT GGGGACCACC AGTACCCGCC 
ATGGCACTAG GAGAAGGAGA CAGACCTTCC CTCACTTTAT ACAGTTGTGG AGGAGAAGGG 
ATTGTCTTAC AGCACAACCC TTGGAAGCTT AGTGGAGAGG CCTTTGACAT CAATAAACTC 
ATCAGGGACA CCAATTCAAT CAAATACAAA TTGCCTGTAC ACACAGAGAT CAGCTGGAAA 
GCAGATGGCA AAATCATGGC TCTTGGCAAT GAAGATGGAT CAATAGAAAT ATTCAAGCTT 
CCTAACTTGA AACTGATCTG CAGCATCCAG CAGCATCATA AGCTTGTGAA TACCATTAGC 
TGGCATCATG AGCATGGCAG CCAGCCAGAA CTGAGCTATC TGATGGCCTC TGGATCCAAC 
AATGCAGTCA TTTATGTGCA CAACCTGCAG ACTGTCATAG AGAGCAATCC TCAATCTCCA 
GTGACCATTA CAGAGCCCTA CCGGACCCTG TCAGGGCACA CAGCCAAGAT CACCAGTCTG 
GCATGGAGCC CACATCATGA TGGAAGGCTG GTATCTGCTT CCTATGATGG TACAGCTCAG 
GTGTGGGATA CTCTCCAGGA AGAGCCTCTG TGCAATTTCC GGGGACATCG AGGTCGACTG 
CTTTGCGTTG TGTGGTCCCC GTTGGATCCA GATTGCATCT ACTCAGGGGC AGATGATTTC 
TGTGTGTATA AATGGCTCAC TTCCATGCAG GAACACTCCC GACCTCCTCA AGGCAAGAAA 
AGTATTGAAT TAGAGAAAAA GCGGCTCTCT CAACCTAAAC CAAAGCCCAA AAAGAAGAAA 
AAGCCCACCT TGAGAGTGCC TGTAAGGCAA GAATTATGTG ATGGGAATGA AGAAGAGAGT 
GTAAAGGAGA GCCTGGGCCC TATTGAAAAT GGCGTGTCAG ACCAGGAGAC TGAGGAGGAA 
GCCCGTGAGC CAGAGCCACC CTGTGGTGTC ACCGCAGCGG TTTCTAAAGA ACCAATTATC 
TGCACTCCAG CAGCCTTAGG TTTTGAAAAG TCAAAAAGCA CCATTAATAA CAAAGTCACT 
TTACTGAAAA AGGAGGTACC TAAAGAGAAG CCAGAAGCCT TAATCAAGAA GCGAAAAGCT 
CGTTCCATGC TTCCACTGAG TACAAGTCTG GACCACAGAT CCAAAGAGGA GCTTCATCAG 
GACTGTTTGG TATTAGCCAC CGCCAAACAC TCCACAGCAG GGCCAAACGA GGATGTGTCT 
ACTGATCTTG AGGAAAGATT TCACCTGGGG CTTTTCACAG ACAGGGCTAC CCTGTACAGA 
ATGATTGAGG TTGAAGGAAA AAGTCACTTG GAAAATGGCC ACCCCGAGTT ATTTCACCAG 
CTCATGCTTT GGAAAGGAGA TCTAAAAGGT GTTCTGCAGA CTGCAGCAGA AAGAGGGGAG 
CTGACAGACA ATCTCGTGGC TATGGCACCA GTCGCCGGTT ACCATGTGTG GGTATGGGCT 
GTAGAAGCTT TTGCCAAACA ACTGTGTTTT CAGGACCAGT ATGTCAAGGC TGCCTCTCAC 
CTGCTTTCTA TCCACAAAGT GTATGAAGCT GTGGAGCTGC TCAAGTCAAA CCATTTCTAC 
AGGGAAGCTA TTGCAATTGC TAAGGCCCGG CTGCGCCCAG AGGACCCAAT CATAAAAGAC 
CTGTACCTCA GCTGGGGAGC CATCCTAGAA AAAGACGGCC ACTATGCCTT AGCAGCAAAA 
TGCTATTTAG GGGCCACTTC AGCTTATGAT GCAGCCAAAG TTCTGGCCAA AAAGGGGGAT 
GCGGCATCAC TTAGGACGGC AGCAGAGTTG GCCTCCATCG TGGGAGAGAA TGAGTTGTCA 
GCTTCCCTGG CTCTAAGATG TGCCCAAGAA CTGCTTCTGG CCCGGAACTG GGTGGGAGCT 
CAGGAGGCCC TGCAGCTCCA CACAAGTCTA AAGGGTCAGA GATTGGTGTT TTGCCTTCTT 
GAGCTACTGT CCAAGCACCT GGAGGAAAAG CAGCTCTCTG AGGGCAAAAG CTCCTTTTCT 
TCTTACAATG ATTGGCCCAC AGACAGCGAA GGGTCCTTTG TGGAGAGGGT GACTGCAGTA 
TGGAAGAGCA CCTTCGGCCT CGACTCCCCA GAGCAGTACC AGGCAGCATT TCAGCAGCTG 
CAAAATATCA AGTACCCATC TGCTACAAGT AACACTCCCT CCAAACAGCT TCTGCTCCAC 
ATTTGCCACG ACCTGACCTT GGCAGTGCTA AGCCAGGAAG CGGGCTCCTG GGACGCAGCT 
GTGGAAGGAT TGCTTCGGGC TGTGATCCGG AGCTACGACT CAGCAAACTT CACCATCATG 
CAGGAAGTGC ACTCAGCCTT TCTTCCGCAG GGCTGTGACC ATCTAAGAGA CAAACTGGGT 
GATCATCAGT CTCCCACCAC ACCAGCTTTC AAAAGTCTGA AGGCCTTTTT TATTTACGGA 
TGTCTATATG AATTCTGGTG GTCTCTCTCT GGGCCTTGCC CGGAGTCCAG TGTCTGGCTA 
AGAGCTGCTC ACAGAAGTAA AACCTCTACT GAACAGAGCC AAGAGGTTGA CAGTGTCAGC 
CCTGAAGAAA CTGACCCCAA ACCTTCCCAG CCAGAGCCAA GCAGGACTCC AGAACTAGAC 
GTGAGACTTT CAGAAGAAGG TGAACAAATG CAGAACGCTT GTAAGGAGCT TTTGTCAGAA 
AAGCATGCTG GTCTCCAAAA TGCACAGAGA ACTGTTGCTG AAGTCCAAGA GACCTTGGCA 
GAAATGATCC GCCAACACCA GAAGAGTCAA CTGTGTAAAT CCACAGCAAA TGGCCCTGAT 
AAGAATGAAC CTGAACAGGA AGGACAGTCC CTCTCTCATC CTCAGAACCA GTGTAAAGAA 
GAAAAAAATG AACCAGTTTC TTTGCCTGAA TTAACCAAAA GACTTACAGA GGCAAATGAG 
AGAATAGCTA AGCTTCCTGA GAGTATTAAG ACCTGGCCTT TCCCGGACGT GCTGGAATGC 
TGCCTCATCC TGCTTCACAT TGGGTCCCAA TGTCCTAGCT TTGTGACCCA GGAAATGCAG 
CAACAGGCTC AAGAGTTCCT TCAGAAGTAC GGCAACACAA AAGTTTACAA ACGACATTAC 
CAGACCCTCT GCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN5 ( XM_010807535.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805837.2 Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa236774
Clone ID Related Accession (Same CDS sequence)	XM_010807535.3
Accession Version	XM_010807535.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	gem-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807535.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCAT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTCTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTC GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC 
TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA 
GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT 
GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA 
TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA 
TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA 
TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG 
TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG 
GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT 
TTTTGGCAAG GTGTCAAGTC CAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC 
TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA 
CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA 
CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT 
GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC 
ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG 
ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA 
ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG 
AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC 
TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT 
CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG 
ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT 
GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT 
CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG 
GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT 
CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC 
AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT 
GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG 
ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA 
GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT 
AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG 
AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG 
GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGC AGGGCCAAAC 
GAGGATGTGT CTACTGATCT TGAGGAAAGA TTTCACCTGG GGCTTTTCAC AGACAGGGCT 
ACCCTGTACA GAATGATTGA GGTTGAAGGA AAAAGTCACT TGGAAAATGG CCACCCCGAG 
TTATTTCACC AGCTCATGCT TTGGAAAGGA GATCTAAAAG GTGTTCTGCA GACTGCAGCA 
GAAAGAGGGG AGCTGACAGA CAATCTCGTG GCTATGGCAC CAGTCGCCGG TTACCATGTG 
TGGGTATGGG CTGTAGAAGC TTTTGCCAAA CAACTGTGTT TTCAGGACCA GTATGTCAAG 
GCTGCCTCTC ACCTGCTTTC TATCCACAAA GTGTATGAAG CTGTGGAGCT GCTCAAGTCA 
AACCATTTCT ACAGGGAAGC TATTGCAATT GCTAAGGCCC GGCTGCGCCC AGAGGACCCA 
ATCATAAAAG ACCTGTACCT CAGCTGGGGA GCCATCCTAG AAAAAGACGG CCACTATGCC 
TTAGCAGCAA AATGCTATTT AGGGGCCACT TCAGCTTATG ATGCAGCCAA AGTTCTGGCC 
AAAAAGGGGG ATGCGGCATC ACTTAGGACG GCAGCAGAGT TGGCCTCCAT CGTGGGAGAG 
AATGAGTTGT CAGCTTCCCT GGCTCTAAGA TGTGCCCAAG AACTGCTTCT GGCCCGGAAC 
TGGGTGGGAG CTCAGGAGGC CCTGCAGCTC CACACAAGTC TAAAGGGTCA GAGATTGGTG 
TTTTGCCTTC TTGAGCTACT GTCCAAGCAC CTGGAGGAAA AGCAGCTCTC TGAGGGCAAA 
AGCTCCTTTT CTTCTTACAA TGATTGGCCC ACAGACAGCG AAGGGTCCTT TGTGGAGAGG 
GTGACTGCAG TATGGAAGAG CACCTTCGGC CTCGACTCCC CAGAGCAGTA CCAGGCAGCA 
TTTCAGCAGC TGCAAAATAT CAAGTACCCA TCTGCTACAA GTAACACTCC CTCCAAACAG 
CTTCTGCTCC ACATTTGCCA CGACCTGACC TTGGCAGTGC TAAGCCAGGA AGCGGGCTCC 
TGGGACGCAG CTGTGGAAGG ATTGCTTCGG GCTGTGATCC GGAGCTACGA CTCAGCAAAC 
TTCACCATCA TGCAGGAAGT GCACTCAGCC TTTCTTCCGC AGGGCTGTGA CCATCTAAGA 
GACAAACTGG GTGATCATCA GTCTCCCACC ACACCAGCTT TCAAAAGTCT GAAGGCCTTT 
TTTATTTACG GATGTCTATA TGAATTCTGG TGGTCTCTCT CTGGGCCTTG CCCGGAGTCC 
AGTGTCTGGC TAAGAGCTGC TCACAGAAGT AAAACCTCTA CTGAACAGAG CCAAGAGGTT 
GACAGTGTCA GCCCTGAAGA AACTGACCCC AAACCTTCCC AGCCAGAGCC AAGCAGGACT 
CCAGAACTAG ACGTGAGACT TTCAGAAGAA GGTGAACAAA TGCAGAACGC TTGTAAGGAG 
CTTTTGTCAG AAAAGCATGC TGGTCTCCAA AATGCACAGA GAACTGTTGC TGAAGTCCAA 
GAGACCTTGG CAGAAATGAT CCGCCAACAC CAGAAGAGTC AACTGTGTAA ATCCACAGCA 
AATGGCCCTG ATAAGAATGA ACCTGAACAG GAAGGACAGT CCCTCTCTCA TCCTCAGAAC 
CAGTGTAAAG AAGAAAAAAA TGAACCAGTT TCTTTGCCTG AATTAACCAA AAGACTTACA 
GAGGCAAATG AGAGAATAGC TAAGCTTCCT GAGAGTATTA AGACCTGGCC TTTCCCGGAC 
GTGCTGGAAT GCTGCCTCAT CCTGCTTCAC ATTGGGTCCC AATGTCCTAG CTTTGTGACC 
CAGGAAATGC AGCAACAGGC TCAAGAGTTC CTTCAGAAGT ACGGCAACAC AAAAGTTTAC 
AAACGACATT ACCAGACCCT CTGCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805837.2
CDS	87..4616
Translation	MGQEPRTLPPSPNWYCSRCSDAVPGGLFGFAARTSVFLVRVGPGADAIPGTPPFRVIGELVGHTEKVSGFTFCHHPGQYNLCATSSDDGTVKIWDVGTKTVVTEHALHQHTISALHWSPRVKDLIVSGDEKGVVFCYWLNRNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVMHRLRGHDDEIHSIAWCPLSGEDCLSINQEENSEEPEMPNGKVITQTPVTKGCYLATGSKDQTIRIWSCSRGRGVMTLKLPFLKRRGGGVDPTVKERLWLTLHWPKDQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSGSSEGQNHSRIVFNLCPLQTEDDKQLLLSISMDRDVKCWDMATLECCWSLPSLGGFAYSLAFSPVDTGCLAIGVGDGMIRVWNTLSIKNNYDVKHFWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYSLAWGPPVPAMALGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFKLPNLKLICSIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLQTVIESNPQSPVTITEPYRTLSGHTAKITSLAWSPHHDGRLVSASYDGTAQVWDTLQEEPLCNFRGHRGRLLCVVWSPLDPDCIYSGADDFCVYKWLTSMQEHSRPPQGKKSIELEKKRLSQPKPKPKKKKKPTLRVPVRQELCDGNEEESVKESLGPIENGVSDQETEEEAREPEPPCGVTAAVSKEPIICTPAALGFEKSKSTINNKVTLLKKEVPKEKPEALIKKRKARSMLPLSTSLDHRSKEELHQDCLVLATAKHSTAGPNEDVSTDLEERFHLGLFTDRATLYRMIEVEGKSHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPVAGYHVWVWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPIIKDLYLSWGAILEKDGHYALAAKCYLGATSAYDAAKVLAKKGDAASLRTAAELASIVGENELSASLALRCAQELLLARNWVGAQEALQLHTSLKGQRLVFCLLELLSKHLEEKQLSEGKSSFSSYNDWPTDSEGSFVERVTAVWKSTFGLDSPEQYQAAFQQLQNIKYPSATSNTPSKQLLLHICHDLTLAVLSQEAGSWDAAVEGLLRAVIRSYDSANFTIMQEVHSAFLPQGCDHLRDKLGDHQSPTTPAFKSLKAFFIYGCLYEFWWSLSGPCPESSVWLRAAHRSKTSTEQSQEVDSVSPEETDPKPSQPEPSRTPELDVRLSEEGEQMQNACKELLSEKHAGLQNAQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEQEGQSLSHPQNQCKEEKNEPVSLPELTKRLTEANERIAKLPESIKTWPFPDVLECCLILLHIGSQCPSFVTQEMQQQAQEFLQKYGNTKVYKRHYQTLCT

Target ORF information:

RefSeq Version	XM_010807535.3
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807535.3

ORF Insert Sequence:

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCAT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTCTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTC GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC 
TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA 
GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT 
GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA 
TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA 
TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA 
TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG 
TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG 
GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT 
TTTTGGCAAG GTGTCAAGTC CAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC 
TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA 
CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA 
CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT 
GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC 
ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG 
ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA 
ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG 
AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC 
TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT 
CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG 
ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT 
GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT 
CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG 
GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT 
CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC 
AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT 
GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG 
ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA 
GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT 
AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG 
AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG 
GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGC AGGGCCAAAC 
GAGGATGTGT CTACTGATCT TGAGGAAAGA TTTCACCTGG GGCTTTTCAC AGACAGGGCT 
ACCCTGTACA GAATGATTGA GGTTGAAGGA AAAAGTCACT TGGAAAATGG CCACCCCGAG 
TTATTTCACC AGCTCATGCT TTGGAAAGGA GATCTAAAAG GTGTTCTGCA GACTGCAGCA 
GAAAGAGGGG AGCTGACAGA CAATCTCGTG GCTATGGCAC CAGTCGCCGG TTACCATGTG 
TGGGTATGGG CTGTAGAAGC TTTTGCCAAA CAACTGTGTT TTCAGGACCA GTATGTCAAG 
GCTGCCTCTC ACCTGCTTTC TATCCACAAA GTGTATGAAG CTGTGGAGCT GCTCAAGTCA 
AACCATTTCT ACAGGGAAGC TATTGCAATT GCTAAGGCCC GGCTGCGCCC AGAGGACCCA 
ATCATAAAAG ACCTGTACCT CAGCTGGGGA GCCATCCTAG AAAAAGACGG CCACTATGCC 
TTAGCAGCAA AATGCTATTT AGGGGCCACT TCAGCTTATG ATGCAGCCAA AGTTCTGGCC 
AAAAAGGGGG ATGCGGCATC ACTTAGGACG GCAGCAGAGT TGGCCTCCAT CGTGGGAGAG 
AATGAGTTGT CAGCTTCCCT GGCTCTAAGA TGTGCCCAAG AACTGCTTCT GGCCCGGAAC 
TGGGTGGGAG CTCAGGAGGC CCTGCAGCTC CACACAAGTC TAAAGGGTCA GAGATTGGTG 
TTTTGCCTTC TTGAGCTACT GTCCAAGCAC CTGGAGGAAA AGCAGCTCTC TGAGGGCAAA 
AGCTCCTTTT CTTCTTACAA TGATTGGCCC ACAGACAGCG AAGGGTCCTT TGTGGAGAGG 
GTGACTGCAG TATGGAAGAG CACCTTCGGC CTCGACTCCC CAGAGCAGTA CCAGGCAGCA 
TTTCAGCAGC TGCAAAATAT CAAGTACCCA TCTGCTACAA GTAACACTCC CTCCAAACAG 
CTTCTGCTCC ACATTTGCCA CGACCTGACC TTGGCAGTGC TAAGCCAGGA AGCGGGCTCC 
TGGGACGCAG CTGTGGAAGG ATTGCTTCGG GCTGTGATCC GGAGCTACGA CTCAGCAAAC 
TTCACCATCA TGCAGGAAGT GCACTCAGCC TTTCTTCCGC AGGGCTGTGA CCATCTAAGA 
GACAAACTGG GTGATCATCA GTCTCCCACC ACACCAGCTT TCAAAAGTCT GAAGGCCTTT 
TTTATTTACG GATGTCTATA TGAATTCTGG TGGTCTCTCT CTGGGCCTTG CCCGGAGTCC 
AGTGTCTGGC TAAGAGCTGC TCACAGAAGT AAAACCTCTA CTGAACAGAG CCAAGAGGTT 
GACAGTGTCA GCCCTGAAGA AACTGACCCC AAACCTTCCC AGCCAGAGCC AAGCAGGACT 
CCAGAACTAG ACGTGAGACT TTCAGAAGAA GGTGAACAAA TGCAGAACGC TTGTAAGGAG 
CTTTTGTCAG AAAAGCATGC TGGTCTCCAA AATGCACAGA GAACTGTTGC TGAAGTCCAA 
GAGACCTTGG CAGAAATGAT CCGCCAACAC CAGAAGAGTC AACTGTGTAA ATCCACAGCA 
AATGGCCCTG ATAAGAATGA ACCTGAACAG GAAGGACAGT CCCTCTCTCA TCCTCAGAAC 
CAGTGTAAAG AAGAAAAAAA TGAACCAGTT TCTTTGCCTG AATTAACCAA AAGACTTACA 
GAGGCAAATG AGAGAATAGC TAAGCTTCCT GAGAGTATTA AGACCTGGCC TTTCCCGGAC 
GTGCTGGAAT GCTGCCTCAT CCTGCTTCAC ATTGGGTCCC AATGTCCTAG CTTTGTGACC 
CAGGAAATGC AGCAACAGGC TCAAGAGTTC CTTCAGAAGT ACGGCAACAC AAAAGTTTAC 
AAACGACATT ACCAGACCCT CTGCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN5 ( XM_010807536.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805838.2 Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X2, mRNA.

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CloneID	OBa236775
Clone ID Related Accession (Same CDS sequence)	XM_010807536.3
Accession Version	XM_010807536.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	gem-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807536.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCAT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTCTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTC GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC 
TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA 
GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT 
GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA 
TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA 
TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA 
TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG 
TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG 
GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT 
TTTTGGCAAG GTGTCAAGTC CAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC 
TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA 
CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA 
CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT 
GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC 
ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG 
ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA 
ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG 
AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC 
TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT 
CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG 
ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT 
GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT 
CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG 
GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT 
CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC 
AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT 
GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG 
ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA 
GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT 
AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG 
AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG 
GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGG GCCAAACGAG 
GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC 
CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA 
TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA 
AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG 
GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT 
GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC 
CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC 
ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA 
GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA 
AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT 
GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG 
GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT 
TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC 
TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG 
ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC GACTCCCCAG AGCAGTACCA GGCAGCATTT 
CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT 
CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG 
GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGCAAACTTC 
ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC 
AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT 
ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT 
GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC 
AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA 
GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT 
TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG 
ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT 
GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG 
TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG 
GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG 
CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG 
GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA 
CGACATTACC AGACCCTCTG CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805838.2
CDS	84..4610
Translation	MGQEPRTLPPSPNWYCSRCSDAVPGGLFGFAARTSVFLVRVGPGADAIPGTPPFRVIGELVGHTEKVSGFTFCHHPGQYNLCATSSDDGTVKIWDVGTKTVVTEHALHQHTISALHWSPRVKDLIVSGDEKGVVFCYWLNRNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVMHRLRGHDDEIHSIAWCPLSGEDCLSINQEENSEEPEMPNGKVITQTPVTKGCYLATGSKDQTIRIWSCSRGRGVMTLKLPFLKRRGGGVDPTVKERLWLTLHWPKDQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSGSSEGQNHSRIVFNLCPLQTEDDKQLLLSISMDRDVKCWDMATLECCWSLPSLGGFAYSLAFSPVDTGCLAIGVGDGMIRVWNTLSIKNNYDVKHFWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYSLAWGPPVPAMALGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFKLPNLKLICSIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLQTVIESNPQSPVTITEPYRTLSGHTAKITSLAWSPHHDGRLVSASYDGTAQVWDTLQEEPLCNFRGHRGRLLCVVWSPLDPDCIYSGADDFCVYKWLTSMQEHSRPPQGKKSIELEKKRLSQPKPKPKKKKKPTLRVPVRQELCDGNEEESVKESLGPIENGVSDQETEEEAREPEPPCGVTAAVSKEPIICTPAALGFEKSKSTINNKVTLLKKEVPKEKPEALIKKRKARSMLPLSTSLDHRSKEELHQDCLVLATAKHSTGPNEDVSTDLEERFHLGLFTDRATLYRMIEVEGKSHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPVAGYHVWVWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPIIKDLYLSWGAILEKDGHYALAAKCYLGATSAYDAAKVLAKKGDAASLRTAAELASIVGENELSASLALRCAQELLLARNWVGAQEALQLHTSLKGQRLVFCLLELLSKHLEEKQLSEGKSSFSSYNDWPTDSEGSFVERVTAVWKSTFGLDSPEQYQAAFQQLQNIKYPSATSNTPSKQLLLHICHDLTLAVLSQEAGSWDAAVEGLLRAVIRSYDSANFTIMQEVHSAFLPQGCDHLRDKLGDHQSPTTPAFKSLKAFFIYGCLYEFWWSLSGPCPESSVWLRAAHRSKTSTEQSQEVDSVSPEETDPKPSQPEPSRTPELDVRLSEEGEQMQNACKELLSEKHAGLQNAQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEQEGQSLSHPQNQCKEEKNEPVSLPELTKRLTEANERIAKLPESIKTWPFPDVLECCLILLHIGSQCPSFVTQEMQQQAQEFLQKYGNTKVYKRHYQTLCT

Target ORF information:

RefSeq Version	XM_010807536.3
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807536.3

ORF Insert Sequence:

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCAT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTCTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTC GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC 
TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA 
GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT 
GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA 
TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA 
TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA 
TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG 
TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG 
GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT 
TTTTGGCAAG GTGTCAAGTC CAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC 
TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA 
CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA 
CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT 
GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC 
ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG 
ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA 
ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG 
AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC 
TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT 
CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG 
ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT 
GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT 
CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG 
GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT 
CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC 
AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT 
GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG 
ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA 
GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT 
AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG 
AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG 
GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGG GCCAAACGAG 
GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC 
CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA 
TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA 
AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG 
GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT 
GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC 
CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC 
ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA 
GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA 
AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT 
GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG 
GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT 
TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC 
TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG 
ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC GACTCCCCAG AGCAGTACCA GGCAGCATTT 
CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT 
CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG 
GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGCAAACTTC 
ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC 
AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT 
ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT 
GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC 
AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA 
GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT 
TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG 
ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT 
GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG 
TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG 
GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG 
CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG 
GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA 
CGACATTACC AGACCCTCTG CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN5 ( XM_010807537.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805839.2 Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X3, mRNA.

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CloneID	OBa236776
Clone ID Related Accession (Same CDS sequence)	XM_010807537.3
Accession Version	XM_010807537.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	gem-associated protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807537.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCAT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTCTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTC GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC 
TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG 
GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT 
AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC 
AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT 
ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT 
CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT 
GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT 
GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT 
TGGCAAGGTG TCAAGTCCAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC 
TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT 
CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA 
GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA 
GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC 
AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC 
AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA 
TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT 
ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT 
GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT 
CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC 
ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT 
ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA 
GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA 
GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA 
GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA 
AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA 
GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT 
GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA 
CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC 
AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG 
CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG 
CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGCAGG GCCAAACGAG 
GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC 
CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA 
TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA 
AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG 
GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT 
GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC 
CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC 
ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA 
GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA 
AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT 
GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG 
GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT 
TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC 
TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG 
ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC GACTCCCCAG AGCAGTACCA GGCAGCATTT 
CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT 
CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG 
GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGCAAACTTC 
ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC 
AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT 
ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT 
GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC 
AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA 
GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT 
TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG 
ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT 
GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG 
TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG 
GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG 
CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG 
GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA 
CGACATTACC AGACCCTCTG CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805839.2
CDS	84..4610
Translation	MGQEPRTLPPSPNWYCSRCSDAVPGGLFGFAARTSVFLVRVGPGADAIPGTPPFRVIGELVGHTEKVSGFTFCHHPGQYNLCATSSDDGTVKIWDVGTKTVVTEHALHQHTISALHWSPRVKDLIVSGDEKGVVFCYWLNRNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVMHRLRGHDDEIHSIAWCPLSGEDCLSINQEENSEPEMPNGKVITQTPVTKGCYLATGSKDQTIRIWSCSRGRGVMTLKLPFLKRRGGGVDPTVKERLWLTLHWPKDQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSGSSEGQNHSRIVFNLCPLQTEDDKQLLLSISMDRDVKCWDMATLECCWSLPSLGGFAYSLAFSPVDTGCLAIGVGDGMIRVWNTLSIKNNYDVKHFWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYSLAWGPPVPAMALGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFKLPNLKLICSIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLQTVIESNPQSPVTITEPYRTLSGHTAKITSLAWSPHHDGRLVSASYDGTAQVWDTLQEEPLCNFRGHRGRLLCVVWSPLDPDCIYSGADDFCVYKWLTSMQEHSRPPQGKKSIELEKKRLSQPKPKPKKKKKPTLRVPVRQELCDGNEEESVKESLGPIENGVSDQETEEEAREPEPPCGVTAAVSKEPIICTPAALGFEKSKSTINNKVTLLKKEVPKEKPEALIKKRKARSMLPLSTSLDHRSKEELHQDCLVLATAKHSTAGPNEDVSTDLEERFHLGLFTDRATLYRMIEVEGKSHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPVAGYHVWVWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPIIKDLYLSWGAILEKDGHYALAAKCYLGATSAYDAAKVLAKKGDAASLRTAAELASIVGENELSASLALRCAQELLLARNWVGAQEALQLHTSLKGQRLVFCLLELLSKHLEEKQLSEGKSSFSSYNDWPTDSEGSFVERVTAVWKSTFGLDSPEQYQAAFQQLQNIKYPSATSNTPSKQLLLHICHDLTLAVLSQEAGSWDAAVEGLLRAVIRSYDSANFTIMQEVHSAFLPQGCDHLRDKLGDHQSPTTPAFKSLKAFFIYGCLYEFWWSLSGPCPESSVWLRAAHRSKTSTEQSQEVDSVSPEETDPKPSQPEPSRTPELDVRLSEEGEQMQNACKELLSEKHAGLQNAQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEQEGQSLSHPQNQCKEEKNEPVSLPELTKRLTEANERIAKLPESIKTWPFPDVLECCLILLHIGSQCPSFVTQEMQQQAQEFLQKYGNTKVYKRHYQTLCT

Target ORF information:

RefSeq Version	XM_010807537.3
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807537.3

ORF Insert Sequence:

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCAT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTCTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTC GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC 
TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG 
GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT 
AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC 
AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT 
ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT 
CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT 
GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT 
GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT 
TGGCAAGGTG TCAAGTCCAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC 
TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT 
CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA 
GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA 
GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC 
AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC 
AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA 
TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT 
ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT 
GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT 
CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC 
ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT 
ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA 
GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA 
GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA 
GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA 
AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA 
GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT 
GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA 
CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC 
AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG 
CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG 
CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGCAGG GCCAAACGAG 
GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC 
CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA 
TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA 
AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG 
GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT 
GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC 
CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC 
ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA 
GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA 
AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT 
GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG 
GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT 
TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC 
TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG 
ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC GACTCCCCAG AGCAGTACCA GGCAGCATTT 
CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT 
CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG 
GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGCAAACTTC 
ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC 
AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT 
ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT 
GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC 
AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA 
GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT 
TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG 
ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT 
GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG 
TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG 
GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG 
CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG 
GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA 
CGACATTACC AGACCCTCTG CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN5 ( XM_010807538.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805840.2 Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa236778
Clone ID Related Accession (Same CDS sequence)	XM_010807538.3
Accession Version	XM_010807538.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	gem-associated protein 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807538.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCAT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTCTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTC GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC 
TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG 
GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT 
AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC 
AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT 
ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT 
CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT 
GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT 
GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT 
TGGCAAGGTG TCAAGTCCAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC 
TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT 
CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA 
GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA 
GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC 
AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC 
AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA 
TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT 
ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT 
GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT 
CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC 
ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT 
ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA 
GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA 
GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA 
GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA 
AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA 
GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT 
GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA 
CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC 
AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG 
CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG 
CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGGGCC AAACGAGGAT 
GTGTCTACTG ATCTTGAGGA AAGATTTCAC CTGGGGCTTT TCACAGACAG GGCTACCCTG 
TACAGAATGA TTGAGGTTGA AGGAAAAAGT CACTTGGAAA ATGGCCACCC CGAGTTATTT 
CACCAGCTCA TGCTTTGGAA AGGAGATCTA AAAGGTGTTC TGCAGACTGC AGCAGAAAGA 
GGGGAGCTGA CAGACAATCT CGTGGCTATG GCACCAGTCG CCGGTTACCA TGTGTGGGTA 
TGGGCTGTAG AAGCTTTTGC CAAACAACTG TGTTTTCAGG ACCAGTATGT CAAGGCTGCC 
TCTCACCTGC TTTCTATCCA CAAAGTGTAT GAAGCTGTGG AGCTGCTCAA GTCAAACCAT 
TTCTACAGGG AAGCTATTGC AATTGCTAAG GCCCGGCTGC GCCCAGAGGA CCCAATCATA 
AAAGACCTGT ACCTCAGCTG GGGAGCCATC CTAGAAAAAG ACGGCCACTA TGCCTTAGCA 
GCAAAATGCT ATTTAGGGGC CACTTCAGCT TATGATGCAG CCAAAGTTCT GGCCAAAAAG 
GGGGATGCGG CATCACTTAG GACGGCAGCA GAGTTGGCCT CCATCGTGGG AGAGAATGAG 
TTGTCAGCTT CCCTGGCTCT AAGATGTGCC CAAGAACTGC TTCTGGCCCG GAACTGGGTG 
GGAGCTCAGG AGGCCCTGCA GCTCCACACA AGTCTAAAGG GTCAGAGATT GGTGTTTTGC 
CTTCTTGAGC TACTGTCCAA GCACCTGGAG GAAAAGCAGC TCTCTGAGGG CAAAAGCTCC 
TTTTCTTCTT ACAATGATTG GCCCACAGAC AGCGAAGGGT CCTTTGTGGA GAGGGTGACT 
GCAGTATGGA AGAGCACCTT CGGCCTCGAC TCCCCAGAGC AGTACCAGGC AGCATTTCAG 
CAGCTGCAAA ATATCAAGTA CCCATCTGCT ACAAGTAACA CTCCCTCCAA ACAGCTTCTG 
CTCCACATTT GCCACGACCT GACCTTGGCA GTGCTAAGCC AGGAAGCGGG CTCCTGGGAC 
GCAGCTGTGG AAGGATTGCT TCGGGCTGTG ATCCGGAGCT ACGACTCAGC AAACTTCACC 
ATCATGCAGG AAGTGCACTC AGCCTTTCTT CCGCAGGGCT GTGACCATCT AAGAGACAAA 
CTGGGTGATC ATCAGTCTCC CACCACACCA GCTTTCAAAA GTCTGAAGGC CTTTTTTATT 
TACGGATGTC TATATGAATT CTGGTGGTCT CTCTCTGGGC CTTGCCCGGA GTCCAGTGTC 
TGGCTAAGAG CTGCTCACAG AAGTAAAACC TCTACTGAAC AGAGCCAAGA GGTTGACAGT 
GTCAGCCCTG AAGAAACTGA CCCCAAACCT TCCCAGCCAG AGCCAAGCAG GACTCCAGAA 
CTAGACGTGA GACTTTCAGA AGAAGGTGAA CAAATGCAGA ACGCTTGTAA GGAGCTTTTG 
TCAGAAAAGC ATGCTGGTCT CCAAAATGCA CAGAGAACTG TTGCTGAAGT CCAAGAGACC 
TTGGCAGAAA TGATCCGCCA ACACCAGAAG AGTCAACTGT GTAAATCCAC AGCAAATGGC 
CCTGATAAGA ATGAACCTGA ACAGGAAGGA CAGTCCCTCT CTCATCCTCA GAACCAGTGT 
AAAGAAGAAA AAAATGAACC AGTTTCTTTG CCTGAATTAA CCAAAAGACT TACAGAGGCA 
AATGAGAGAA TAGCTAAGCT TCCTGAGAGT ATTAAGACCT GGCCTTTCCC GGACGTGCTG 
GAATGCTGCC TCATCCTGCT TCACATTGGG TCCCAATGTC CTAGCTTTGT GACCCAGGAA 
ATGCAGCAAC AGGCTCAAGA GTTCCTTCAG AAGTACGGCA ACACAAAAGT TTACAAACGA 
CATTACCAGA CCCTCTGCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805840.2
CDS	84..4607
Translation	MGQEPRTLPPSPNWYCSRCSDAVPGGLFGFAARTSVFLVRVGPGADAIPGTPPFRVIGELVGHTEKVSGFTFCHHPGQYNLCATSSDDGTVKIWDVGTKTVVTEHALHQHTISALHWSPRVKDLIVSGDEKGVVFCYWLNRNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVMHRLRGHDDEIHSIAWCPLSGEDCLSINQEENSEPEMPNGKVITQTPVTKGCYLATGSKDQTIRIWSCSRGRGVMTLKLPFLKRRGGGVDPTVKERLWLTLHWPKDQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSGSSEGQNHSRIVFNLCPLQTEDDKQLLLSISMDRDVKCWDMATLECCWSLPSLGGFAYSLAFSPVDTGCLAIGVGDGMIRVWNTLSIKNNYDVKHFWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYSLAWGPPVPAMALGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFKLPNLKLICSIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLQTVIESNPQSPVTITEPYRTLSGHTAKITSLAWSPHHDGRLVSASYDGTAQVWDTLQEEPLCNFRGHRGRLLCVVWSPLDPDCIYSGADDFCVYKWLTSMQEHSRPPQGKKSIELEKKRLSQPKPKPKKKKKPTLRVPVRQELCDGNEEESVKESLGPIENGVSDQETEEEAREPEPPCGVTAAVSKEPIICTPAALGFEKSKSTINNKVTLLKKEVPKEKPEALIKKRKARSMLPLSTSLDHRSKEELHQDCLVLATAKHSTGPNEDVSTDLEERFHLGLFTDRATLYRMIEVEGKSHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPVAGYHVWVWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPIIKDLYLSWGAILEKDGHYALAAKCYLGATSAYDAAKVLAKKGDAASLRTAAELASIVGENELSASLALRCAQELLLARNWVGAQEALQLHTSLKGQRLVFCLLELLSKHLEEKQLSEGKSSFSSYNDWPTDSEGSFVERVTAVWKSTFGLDSPEQYQAAFQQLQNIKYPSATSNTPSKQLLLHICHDLTLAVLSQEAGSWDAAVEGLLRAVIRSYDSANFTIMQEVHSAFLPQGCDHLRDKLGDHQSPTTPAFKSLKAFFIYGCLYEFWWSLSGPCPESSVWLRAAHRSKTSTEQSQEVDSVSPEETDPKPSQPEPSRTPELDVRLSEEGEQMQNACKELLSEKHAGLQNAQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEQEGQSLSHPQNQCKEEKNEPVSLPELTKRLTEANERIAKLPESIKTWPFPDVLECCLILLHIGSQCPSFVTQEMQQQAQEFLQKYGNTKVYKRHYQTLCT

Target ORF information:

RefSeq Version	XM_010807538.3
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807538.3

ORF Insert Sequence:

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCAT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTCTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTC GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC 
TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG 
GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT 
AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC 
AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT 
ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT 
CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT 
GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT 
GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT 
TGGCAAGGTG TCAAGTCCAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC 
TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT 
CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA 
GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA 
GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC 
AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC 
AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA 
TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT 
ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT 
GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT 
CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC 
ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT 
ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA 
GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA 
GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA 
GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA 
AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA 
GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT 
GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA 
CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC 
AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG 
CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG 
CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGGGCC AAACGAGGAT 
GTGTCTACTG ATCTTGAGGA AAGATTTCAC CTGGGGCTTT TCACAGACAG GGCTACCCTG 
TACAGAATGA TTGAGGTTGA AGGAAAAAGT CACTTGGAAA ATGGCCACCC CGAGTTATTT 
CACCAGCTCA TGCTTTGGAA AGGAGATCTA AAAGGTGTTC TGCAGACTGC AGCAGAAAGA 
GGGGAGCTGA CAGACAATCT CGTGGCTATG GCACCAGTCG CCGGTTACCA TGTGTGGGTA 
TGGGCTGTAG AAGCTTTTGC CAAACAACTG TGTTTTCAGG ACCAGTATGT CAAGGCTGCC 
TCTCACCTGC TTTCTATCCA CAAAGTGTAT GAAGCTGTGG AGCTGCTCAA GTCAAACCAT 
TTCTACAGGG AAGCTATTGC AATTGCTAAG GCCCGGCTGC GCCCAGAGGA CCCAATCATA 
AAAGACCTGT ACCTCAGCTG GGGAGCCATC CTAGAAAAAG ACGGCCACTA TGCCTTAGCA 
GCAAAATGCT ATTTAGGGGC CACTTCAGCT TATGATGCAG CCAAAGTTCT GGCCAAAAAG 
GGGGATGCGG CATCACTTAG GACGGCAGCA GAGTTGGCCT CCATCGTGGG AGAGAATGAG 
TTGTCAGCTT CCCTGGCTCT AAGATGTGCC CAAGAACTGC TTCTGGCCCG GAACTGGGTG 
GGAGCTCAGG AGGCCCTGCA GCTCCACACA AGTCTAAAGG GTCAGAGATT GGTGTTTTGC 
CTTCTTGAGC TACTGTCCAA GCACCTGGAG GAAAAGCAGC TCTCTGAGGG CAAAAGCTCC 
TTTTCTTCTT ACAATGATTG GCCCACAGAC AGCGAAGGGT CCTTTGTGGA GAGGGTGACT 
GCAGTATGGA AGAGCACCTT CGGCCTCGAC TCCCCAGAGC AGTACCAGGC AGCATTTCAG 
CAGCTGCAAA ATATCAAGTA CCCATCTGCT ACAAGTAACA CTCCCTCCAA ACAGCTTCTG 
CTCCACATTT GCCACGACCT GACCTTGGCA GTGCTAAGCC AGGAAGCGGG CTCCTGGGAC 
GCAGCTGTGG AAGGATTGCT TCGGGCTGTG ATCCGGAGCT ACGACTCAGC AAACTTCACC 
ATCATGCAGG AAGTGCACTC AGCCTTTCTT CCGCAGGGCT GTGACCATCT AAGAGACAAA 
CTGGGTGATC ATCAGTCTCC CACCACACCA GCTTTCAAAA GTCTGAAGGC CTTTTTTATT 
TACGGATGTC TATATGAATT CTGGTGGTCT CTCTCTGGGC CTTGCCCGGA GTCCAGTGTC 
TGGCTAAGAG CTGCTCACAG AAGTAAAACC TCTACTGAAC AGAGCCAAGA GGTTGACAGT 
GTCAGCCCTG AAGAAACTGA CCCCAAACCT TCCCAGCCAG AGCCAAGCAG GACTCCAGAA 
CTAGACGTGA GACTTTCAGA AGAAGGTGAA CAAATGCAGA ACGCTTGTAA GGAGCTTTTG 
TCAGAAAAGC ATGCTGGTCT CCAAAATGCA CAGAGAACTG TTGCTGAAGT CCAAGAGACC 
TTGGCAGAAA TGATCCGCCA ACACCAGAAG AGTCAACTGT GTAAATCCAC AGCAAATGGC 
CCTGATAAGA ATGAACCTGA ACAGGAAGGA CAGTCCCTCT CTCATCCTCA GAACCAGTGT 
AAAGAAGAAA AAAATGAACC AGTTTCTTTG CCTGAATTAA CCAAAAGACT TACAGAGGCA 
AATGAGAGAA TAGCTAAGCT TCCTGAGAGT ATTAAGACCT GGCCTTTCCC GGACGTGCTG 
GAATGCTGCC TCATCCTGCT TCACATTGGG TCCCAATGTC CTAGCTTTGT GACCCAGGAA 
ATGCAGCAAC AGGCTCAAGA GTTCCTTCAG AAGTACGGCA ACACAAAAGT TTACAAACGA 
CATTACCAGA CCCTCTGCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN5 ( XM_010807535.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805837.1 Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa52456
Clone ID Related Accession (Same CDS sequence)	XM_010807535.2 , XM_010798518.2
Accession Version	XM_010807535.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	gem-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807535.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC 
TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA 
GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT 
GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA 
TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA 
TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA 
TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG 
TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG 
GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT 
TTTTGGCAAG GTGTCAAGTC GAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC 
TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA 
CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA 
CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT 
GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC 
ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG 
ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA 
ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG 
AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC 
TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT 
CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG 
ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT 
GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT 
CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG 
GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT 
CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC 
AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT 
GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG 
ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA 
GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT 
AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG 
AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG 
GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGC AGGGCCAAAC 
GAGGATGTGT CTACTGATCT TGAGGAAAGA TTTCACCTGG GGCTTTTCAC AGACAGGGCT 
ACCCTGTACA GAATGATTGA GGTTGAAGGA AAAAGTCACT TGGAAAATGG CCACCCCGAG 
TTATTTCACC AGCTCATGCT TTGGAAAGGA GATCTAAAAG GTGTTCTGCA GACTGCAGCA 
GAAAGAGGGG AGCTGACAGA CAATCTCGTG GCTATGGCAC CAGTCGCCGG TTACCATGTG 
TGGGTATGGG CTGTAGAAGC TTTTGCCAAA CAACTGTGTT TTCAGGACCA GTATGTCAAG 
GCTGCCTCTC ACCTGCTTTC TATCCACAAA GTGTATGAAG CTGTGGAGCT GCTCAAGTCA 
AACCATTTCT ACAGGGAAGC TATTGCAATT GCTAAGGCCC GGCTGCGCCC AGAGGACCCA 
ATCATAAAAG ACCTGTACCT CAGCTGGGGA GCCATCCTAG AAAAAGACGG CCACTATGCC 
TTAGCAGCAA AATGCTATTT AGGGGCCACT TCAGCTTATG ATGCAGCCAA AGTTCTGGCC 
AAAAAGGGGG ATGCGGCATC ACTTAGGACG GCAGCAGAGT TGGCCTCCAT CGTGGGAGAG 
AATGAGTTGT CAGCTTCCCT GGCTCTAAGA TGTGCCCAAG AACTGCTTCT GGCCCGGAAC 
TGGGTGGGAG CTCAGGAGGC CCTGCAGCTC CACACAAGTC TAAAGGGTCA GAGATTGGTG 
TTTTGCCTTC TTGAGCTACT GTCCAAGCAC CTGGAGGAAA AGCAGCTCTC TGAGGGCAAA 
AGCTCCTTTT CTTCTTACAA TGATTGGCCC ACAGACAGCG AAGGGTCCTT TGTGGAGAGG 
GTGACTGCAG TATGGAAGAG CACCTTCGGC CTCAACTCCC CAGAGCAGTA CCAGGCAGCA 
TTTCAGCAGC TGCAAAATAT CAAGTACCCA TCTGCTACAA GTAACACTCC CTCCAAACAG 
CTTCTGCTCC ACATTTGCCA CGACCTGACC TTGGCAGTGC TAAGCCAGGA AGCGGGCTCC 
TGGGACGCAG CTGTGGAAGG ATTGCTTCGG GCTGTGATCC GGAGCTACGA CTCAGGAAAC 
TTCACCATCA TGCAGGAAGT GCACTCAGCC TTTCTTCCGC AGGGCTGTGA CCATCTAAGA 
GACAAACTGG GTGATCATCA GTCTCCCACC ACACCAGCTT TCAAAAGTCT GAAGGCCTTT 
TTTATTTACG GATGTCTATA TGAATTCTGG TGGTCTCTCT CTGGGCCTTG CCCGGAGTCC 
AGTGTCTGGC TAAGAGCTGC TCACAGAAGT AAAACCTCTA CTGAACAGAG CCAAGAGGTT 
GACAGTGTCA GCCCTGAAGA AACTGACCCC AAACCTTCCC AGCCAGAGCC AAGCAGGACT 
CCAGAACTAG ACGTGAGACT TTCAGAAGAA GGTGAACAAA TGCAGAACGC TTGTAAGGAG 
CTTTTGTCAG AAAAGCATGC TGGTCTCCAA AATGCACAGA GAACTGTTGC TGAAGTCCAA 
GAGACCTTGG CAGAAATGAT CCGCCAACAC CAGAAGAGTC AACTGTGTAA ATCCACAGCA 
AATGGCCCTG ATAAGAATGA ACCTGAACAG GAAGGACAGT CCCTCTCTCA TCCTCAGAAC 
CAGTGTAAAG AAGAAAAAAA TGAACCAGTT TCTTTGCCTG AATTAACCAA AAGACTTACA 
GAGGCAAATG AGAGAATAGC TAAGCTTCCT GAGAGTATTA AGACCTGGCC TTTCCCGGAC 
GTGCTGGAAT GCTGCCTCAT CCTGCTTCAC ATTGGGTCCC AATGTCCTAG CTTTGTGACC 
CAGGAAATGC AGCAACAGGC TCAAGAGTTC CTTCAGAAGT ACGGCAACAC AAAAGTTTAC 
AAACGACATT ACCAGACCCT CTGCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805837.1
CDS	77..4606
Translation	MGQEPRTLPPSPNWYCSRCSDAVPGGLFGFAARTSVFLVRVGPGADAIPGTPPFRVVGELVGHTEKVSGFTFCHHPGQYNLCATSSDDGTVKIWDVGTKTVVTEHALHQHTISALHWSPRVKDLIVSGDEKGVVFCYWLNRNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVMHRLRGHDDEIHSIAWCPLLGEDCLSINQEENSEEPEMPNGKVITQTPVTKGCYLATGSKDQTIRIWSCSRGRGVMTLKLPFLKRRGGGVDPTVKERLWLTLHWPKDQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSGSSEGQNHSRIVFNLCPLQTEDDKQLLLSISMDRDVKCWDMATLECCWSLPSLGGFAYSLAFSPVDTGCLAIGVGDGMIRVWNTLSIKNNYDVKHFWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYSLAWGPPVPAMALGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFKLPNLKLICSIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLQTVIESNPQSPVTITEPYRTLSGHTAKITSLAWSPHHDGRLVSASYDGTAQVWDTLQEEPLCNFRGHRGRLLCVVWSPLDPDCIYSGADDFCVYKWLTSMQEHSRPPQGKKSIELEKKRLSQPKPKPKKKKKPTLRVPVRQELCDGNEEESVKESLGPIENGVSDQETEEEAREPEPPCGVTAAVSKEPIICTPAALGFEKSKSTINNKVTLLKKEVPKEKPEALIKKRKARSMLPLSTSLDHRSKEELHQDCLVLATAKHSTAGPNEDVSTDLEERFHLGLFTDRATLYRMIEVEGKSHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPVAGYHVWVWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPIIKDLYLSWGAILEKDGHYALAAKCYLGATSAYDAAKVLAKKGDAASLRTAAELASIVGENELSASLALRCAQELLLARNWVGAQEALQLHTSLKGQRLVFCLLELLSKHLEEKQLSEGKSSFSSYNDWPTDSEGSFVERVTAVWKSTFGLNSPEQYQAAFQQLQNIKYPSATSNTPSKQLLLHICHDLTLAVLSQEAGSWDAAVEGLLRAVIRSYDSGNFTIMQEVHSAFLPQGCDHLRDKLGDHQSPTTPAFKSLKAFFIYGCLYEFWWSLSGPCPESSVWLRAAHRSKTSTEQSQEVDSVSPEETDPKPSQPEPSRTPELDVRLSEEGEQMQNACKELLSEKHAGLQNAQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEQEGQSLSHPQNQCKEEKNEPVSLPELTKRLTEANERIAKLPESIKTWPFPDVLECCLILLHIGSQCPSFVTQEMQQQAQEFLQKYGNTKVYKRHYQTLCT

Target ORF information:

RefSeq Version	XM_010807535.2
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807535.2

ORF Insert Sequence:

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC 
TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA 
GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT 
GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA 
TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA 
TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA 
TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG 
TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG 
GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT 
TTTTGGCAAG GTGTCAAGTC GAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC 
TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA 
CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA 
CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT 
GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC 
ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG 
ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA 
ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG 
AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC 
TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT 
CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG 
ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT 
GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT 
CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG 
GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT 
CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC 
AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT 
GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG 
ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA 
GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT 
AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG 
AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG 
GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGC AGGGCCAAAC 
GAGGATGTGT CTACTGATCT TGAGGAAAGA TTTCACCTGG GGCTTTTCAC AGACAGGGCT 
ACCCTGTACA GAATGATTGA GGTTGAAGGA AAAAGTCACT TGGAAAATGG CCACCCCGAG 
TTATTTCACC AGCTCATGCT TTGGAAAGGA GATCTAAAAG GTGTTCTGCA GACTGCAGCA 
GAAAGAGGGG AGCTGACAGA CAATCTCGTG GCTATGGCAC CAGTCGCCGG TTACCATGTG 
TGGGTATGGG CTGTAGAAGC TTTTGCCAAA CAACTGTGTT TTCAGGACCA GTATGTCAAG 
GCTGCCTCTC ACCTGCTTTC TATCCACAAA GTGTATGAAG CTGTGGAGCT GCTCAAGTCA 
AACCATTTCT ACAGGGAAGC TATTGCAATT GCTAAGGCCC GGCTGCGCCC AGAGGACCCA 
ATCATAAAAG ACCTGTACCT CAGCTGGGGA GCCATCCTAG AAAAAGACGG CCACTATGCC 
TTAGCAGCAA AATGCTATTT AGGGGCCACT TCAGCTTATG ATGCAGCCAA AGTTCTGGCC 
AAAAAGGGGG ATGCGGCATC ACTTAGGACG GCAGCAGAGT TGGCCTCCAT CGTGGGAGAG 
AATGAGTTGT CAGCTTCCCT GGCTCTAAGA TGTGCCCAAG AACTGCTTCT GGCCCGGAAC 
TGGGTGGGAG CTCAGGAGGC CCTGCAGCTC CACACAAGTC TAAAGGGTCA GAGATTGGTG 
TTTTGCCTTC TTGAGCTACT GTCCAAGCAC CTGGAGGAAA AGCAGCTCTC TGAGGGCAAA 
AGCTCCTTTT CTTCTTACAA TGATTGGCCC ACAGACAGCG AAGGGTCCTT TGTGGAGAGG 
GTGACTGCAG TATGGAAGAG CACCTTCGGC CTCAACTCCC CAGAGCAGTA CCAGGCAGCA 
TTTCAGCAGC TGCAAAATAT CAAGTACCCA TCTGCTACAA GTAACACTCC CTCCAAACAG 
CTTCTGCTCC ACATTTGCCA CGACCTGACC TTGGCAGTGC TAAGCCAGGA AGCGGGCTCC 
TGGGACGCAG CTGTGGAAGG ATTGCTTCGG GCTGTGATCC GGAGCTACGA CTCAGGAAAC 
TTCACCATCA TGCAGGAAGT GCACTCAGCC TTTCTTCCGC AGGGCTGTGA CCATCTAAGA 
GACAAACTGG GTGATCATCA GTCTCCCACC ACACCAGCTT TCAAAAGTCT GAAGGCCTTT 
TTTATTTACG GATGTCTATA TGAATTCTGG TGGTCTCTCT CTGGGCCTTG CCCGGAGTCC 
AGTGTCTGGC TAAGAGCTGC TCACAGAAGT AAAACCTCTA CTGAACAGAG CCAAGAGGTT 
GACAGTGTCA GCCCTGAAGA AACTGACCCC AAACCTTCCC AGCCAGAGCC AAGCAGGACT 
CCAGAACTAG ACGTGAGACT TTCAGAAGAA GGTGAACAAA TGCAGAACGC TTGTAAGGAG 
CTTTTGTCAG AAAAGCATGC TGGTCTCCAA AATGCACAGA GAACTGTTGC TGAAGTCCAA 
GAGACCTTGG CAGAAATGAT CCGCCAACAC CAGAAGAGTC AACTGTGTAA ATCCACAGCA 
AATGGCCCTG ATAAGAATGA ACCTGAACAG GAAGGACAGT CCCTCTCTCA TCCTCAGAAC 
CAGTGTAAAG AAGAAAAAAA TGAACCAGTT TCTTTGCCTG AATTAACCAA AAGACTTACA 
GAGGCAAATG AGAGAATAGC TAAGCTTCCT GAGAGTATTA AGACCTGGCC TTTCCCGGAC 
GTGCTGGAAT GCTGCCTCAT CCTGCTTCAC ATTGGGTCCC AATGTCCTAG CTTTGTGACC 
CAGGAAATGC AGCAACAGGC TCAAGAGTTC CTTCAGAAGT ACGGCAACAC AAAAGTTTAC 
AAACGACATT ACCAGACCCT CTGCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN5 ( XM_010798518.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010796820.2 Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X1, mRNA.

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CloneID	OBa52456
Clone ID Related Accession (Same CDS sequence)	XM_010807535.2 , XM_010798518.2
Accession Version	XM_010798518.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	gem-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC 
TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA 
GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT 
GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA 
TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA 
TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA 
TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG 
TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG 
GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT 
TTTTGGCAAG GTGTCAAGTC GAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC 
TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA 
CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA 
CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT 
GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC 
ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG 
ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA 
ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG 
AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC 
TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT 
CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG 
ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT 
GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT 
CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG 
GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT 
CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC 
AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT 
GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG 
ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA 
GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT 
AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG 
AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG 
GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGC AGGGCCAAAC 
GAGGATGTGT CTACTGATCT TGAGGAAAGA TTTCACCTGG GGCTTTTCAC AGACAGGGCT 
ACCCTGTACA GAATGATTGA GGTTGAAGGA AAAAGTCACT TGGAAAATGG CCACCCCGAG 
TTATTTCACC AGCTCATGCT TTGGAAAGGA GATCTAAAAG GTGTTCTGCA GACTGCAGCA 
GAAAGAGGGG AGCTGACAGA CAATCTCGTG GCTATGGCAC CAGTCGCCGG TTACCATGTG 
TGGGTATGGG CTGTAGAAGC TTTTGCCAAA CAACTGTGTT TTCAGGACCA GTATGTCAAG 
GCTGCCTCTC ACCTGCTTTC TATCCACAAA GTGTATGAAG CTGTGGAGCT GCTCAAGTCA 
AACCATTTCT ACAGGGAAGC TATTGCAATT GCTAAGGCCC GGCTGCGCCC AGAGGACCCA 
ATCATAAAAG ACCTGTACCT CAGCTGGGGA GCCATCCTAG AAAAAGACGG CCACTATGCC 
TTAGCAGCAA AATGCTATTT AGGGGCCACT TCAGCTTATG ATGCAGCCAA AGTTCTGGCC 
AAAAAGGGGG ATGCGGCATC ACTTAGGACG GCAGCAGAGT TGGCCTCCAT CGTGGGAGAG 
AATGAGTTGT CAGCTTCCCT GGCTCTAAGA TGTGCCCAAG AACTGCTTCT GGCCCGGAAC 
TGGGTGGGAG CTCAGGAGGC CCTGCAGCTC CACACAAGTC TAAAGGGTCA GAGATTGGTG 
TTTTGCCTTC TTGAGCTACT GTCCAAGCAC CTGGAGGAAA AGCAGCTCTC TGAGGGCAAA 
AGCTCCTTTT CTTCTTACAA TGATTGGCCC ACAGACAGCG AAGGGTCCTT TGTGGAGAGG 
GTGACTGCAG TATGGAAGAG CACCTTCGGC CTCAACTCCC CAGAGCAGTA CCAGGCAGCA 
TTTCAGCAGC TGCAAAATAT CAAGTACCCA TCTGCTACAA GTAACACTCC CTCCAAACAG 
CTTCTGCTCC ACATTTGCCA CGACCTGACC TTGGCAGTGC TAAGCCAGGA AGCGGGCTCC 
TGGGACGCAG CTGTGGAAGG ATTGCTTCGG GCTGTGATCC GGAGCTACGA CTCAGGAAAC 
TTCACCATCA TGCAGGAAGT GCACTCAGCC TTTCTTCCGC AGGGCTGTGA CCATCTAAGA 
GACAAACTGG GTGATCATCA GTCTCCCACC ACACCAGCTT TCAAAAGTCT GAAGGCCTTT 
TTTATTTACG GATGTCTATA TGAATTCTGG TGGTCTCTCT CTGGGCCTTG CCCGGAGTCC 
AGTGTCTGGC TAAGAGCTGC TCACAGAAGT AAAACCTCTA CTGAACAGAG CCAAGAGGTT 
GACAGTGTCA GCCCTGAAGA AACTGACCCC AAACCTTCCC AGCCAGAGCC AAGCAGGACT 
CCAGAACTAG ACGTGAGACT TTCAGAAGAA GGTGAACAAA TGCAGAACGC TTGTAAGGAG 
CTTTTGTCAG AAAAGCATGC TGGTCTCCAA AATGCACAGA GAACTGTTGC TGAAGTCCAA 
GAGACCTTGG CAGAAATGAT CCGCCAACAC CAGAAGAGTC AACTGTGTAA ATCCACAGCA 
AATGGCCCTG ATAAGAATGA ACCTGAACAG GAAGGACAGT CCCTCTCTCA TCCTCAGAAC 
CAGTGTAAAG AAGAAAAAAA TGAACCAGTT TCTTTGCCTG AATTAACCAA AAGACTTACA 
GAGGCAAATG AGAGAATAGC TAAGCTTCCT GAGAGTATTA AGACCTGGCC TTTCCCGGAC 
GTGCTGGAAT GCTGCCTCAT CCTGCTTCAC ATTGGGTCCC AATGTCCTAG CTTTGTGACC 
CAGGAAATGC AGCAACAGGC TCAAGAGTTC CTTCAGAAGT ACGGCAACAC AAAAGTTTAC 
AAACGACATT ACCAGACCCT CTGCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010796820.2
CDS	77..4606
Translation	MGQEPRTLPPSPNWYCSRCSDAVPGGLFGFAARTSVFLVRVGPGADAIPGTPPFRVVGELVGHTEKVSGFTFCHHPGQYNLCATSSDDGTVKIWDVGTKTVVTEHALHQHTISALHWSPRVKDLIVSGDEKGVVFCYWLNRNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVMHRLRGHDDEIHSIAWCPLLGEDCLSINQEENSEEPEMPNGKVITQTPVTKGCYLATGSKDQTIRIWSCSRGRGVMTLKLPFLKRRGGGVDPTVKERLWLTLHWPKDQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSGSSEGQNHSRIVFNLCPLQTEDDKQLLLSISMDRDVKCWDMATLECCWSLPSLGGFAYSLAFSPVDTGCLAIGVGDGMIRVWNTLSIKNNYDVKHFWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYSLAWGPPVPAMALGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFKLPNLKLICSIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLQTVIESNPQSPVTITEPYRTLSGHTAKITSLAWSPHHDGRLVSASYDGTAQVWDTLQEEPLCNFRGHRGRLLCVVWSPLDPDCIYSGADDFCVYKWLTSMQEHSRPPQGKKSIELEKKRLSQPKPKPKKKKKPTLRVPVRQELCDGNEEESVKESLGPIENGVSDQETEEEAREPEPPCGVTAAVSKEPIICTPAALGFEKSKSTINNKVTLLKKEVPKEKPEALIKKRKARSMLPLSTSLDHRSKEELHQDCLVLATAKHSTAGPNEDVSTDLEERFHLGLFTDRATLYRMIEVEGKSHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPVAGYHVWVWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPIIKDLYLSWGAILEKDGHYALAAKCYLGATSAYDAAKVLAKKGDAASLRTAAELASIVGENELSASLALRCAQELLLARNWVGAQEALQLHTSLKGQRLVFCLLELLSKHLEEKQLSEGKSSFSSYNDWPTDSEGSFVERVTAVWKSTFGLNSPEQYQAAFQQLQNIKYPSATSNTPSKQLLLHICHDLTLAVLSQEAGSWDAAVEGLLRAVIRSYDSGNFTIMQEVHSAFLPQGCDHLRDKLGDHQSPTTPAFKSLKAFFIYGCLYEFWWSLSGPCPESSVWLRAAHRSKTSTEQSQEVDSVSPEETDPKPSQPEPSRTPELDVRLSEEGEQMQNACKELLSEKHAGLQNAQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEQEGQSLSHPQNQCKEEKNEPVSLPELTKRLTEANERIAKLPESIKTWPFPDVLECCLILLHIGSQCPSFVTQEMQQQAQEFLQKYGNTKVYKRHYQTLCT

Target ORF information:

RefSeq Version	XM_010798518.2
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010798518.2

ORF Insert Sequence:

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC 
TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA 
GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT 
GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA 
TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA 
TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA 
TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG 
TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG 
GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT 
TTTTGGCAAG GTGTCAAGTC GAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC 
TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA 
CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA 
CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT 
GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC 
ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG 
ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA 
ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG 
AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC 
TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT 
CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG 
ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT 
GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT 
CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG 
GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT 
CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC 
AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT 
GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG 
ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA 
GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT 
AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG 
AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG 
GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGC AGGGCCAAAC 
GAGGATGTGT CTACTGATCT TGAGGAAAGA TTTCACCTGG GGCTTTTCAC AGACAGGGCT 
ACCCTGTACA GAATGATTGA GGTTGAAGGA AAAAGTCACT TGGAAAATGG CCACCCCGAG 
TTATTTCACC AGCTCATGCT TTGGAAAGGA GATCTAAAAG GTGTTCTGCA GACTGCAGCA 
GAAAGAGGGG AGCTGACAGA CAATCTCGTG GCTATGGCAC CAGTCGCCGG TTACCATGTG 
TGGGTATGGG CTGTAGAAGC TTTTGCCAAA CAACTGTGTT TTCAGGACCA GTATGTCAAG 
GCTGCCTCTC ACCTGCTTTC TATCCACAAA GTGTATGAAG CTGTGGAGCT GCTCAAGTCA 
AACCATTTCT ACAGGGAAGC TATTGCAATT GCTAAGGCCC GGCTGCGCCC AGAGGACCCA 
ATCATAAAAG ACCTGTACCT CAGCTGGGGA GCCATCCTAG AAAAAGACGG CCACTATGCC 
TTAGCAGCAA AATGCTATTT AGGGGCCACT TCAGCTTATG ATGCAGCCAA AGTTCTGGCC 
AAAAAGGGGG ATGCGGCATC ACTTAGGACG GCAGCAGAGT TGGCCTCCAT CGTGGGAGAG 
AATGAGTTGT CAGCTTCCCT GGCTCTAAGA TGTGCCCAAG AACTGCTTCT GGCCCGGAAC 
TGGGTGGGAG CTCAGGAGGC CCTGCAGCTC CACACAAGTC TAAAGGGTCA GAGATTGGTG 
TTTTGCCTTC TTGAGCTACT GTCCAAGCAC CTGGAGGAAA AGCAGCTCTC TGAGGGCAAA 
AGCTCCTTTT CTTCTTACAA TGATTGGCCC ACAGACAGCG AAGGGTCCTT TGTGGAGAGG 
GTGACTGCAG TATGGAAGAG CACCTTCGGC CTCAACTCCC CAGAGCAGTA CCAGGCAGCA 
TTTCAGCAGC TGCAAAATAT CAAGTACCCA TCTGCTACAA GTAACACTCC CTCCAAACAG 
CTTCTGCTCC ACATTTGCCA CGACCTGACC TTGGCAGTGC TAAGCCAGGA AGCGGGCTCC 
TGGGACGCAG CTGTGGAAGG ATTGCTTCGG GCTGTGATCC GGAGCTACGA CTCAGGAAAC 
TTCACCATCA TGCAGGAAGT GCACTCAGCC TTTCTTCCGC AGGGCTGTGA CCATCTAAGA 
GACAAACTGG GTGATCATCA GTCTCCCACC ACACCAGCTT TCAAAAGTCT GAAGGCCTTT 
TTTATTTACG GATGTCTATA TGAATTCTGG TGGTCTCTCT CTGGGCCTTG CCCGGAGTCC 
AGTGTCTGGC TAAGAGCTGC TCACAGAAGT AAAACCTCTA CTGAACAGAG CCAAGAGGTT 
GACAGTGTCA GCCCTGAAGA AACTGACCCC AAACCTTCCC AGCCAGAGCC AAGCAGGACT 
CCAGAACTAG ACGTGAGACT TTCAGAAGAA GGTGAACAAA TGCAGAACGC TTGTAAGGAG 
CTTTTGTCAG AAAAGCATGC TGGTCTCCAA AATGCACAGA GAACTGTTGC TGAAGTCCAA 
GAGACCTTGG CAGAAATGAT CCGCCAACAC CAGAAGAGTC AACTGTGTAA ATCCACAGCA 
AATGGCCCTG ATAAGAATGA ACCTGAACAG GAAGGACAGT CCCTCTCTCA TCCTCAGAAC 
CAGTGTAAAG AAGAAAAAAA TGAACCAGTT TCTTTGCCTG AATTAACCAA AAGACTTACA 
GAGGCAAATG AGAGAATAGC TAAGCTTCCT GAGAGTATTA AGACCTGGCC TTTCCCGGAC 
GTGCTGGAAT GCTGCCTCAT CCTGCTTCAC ATTGGGTCCC AATGTCCTAG CTTTGTGACC 
CAGGAAATGC AGCAACAGGC TCAAGAGTTC CTTCAGAAGT ACGGCAACAC AAAAGTTTAC 
AAACGACATT ACCAGACCCT CTGCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN5 ( XM_010798519.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010796821.2 Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X2, mRNA.

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CloneID	OBa52457
Clone ID Related Accession (Same CDS sequence)	XM_010798519.2 , XM_010807536.2
Accession Version	XM_010798519.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	gem-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC 
TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA 
GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT 
GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA 
TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA 
TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA 
TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG 
TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG 
GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT 
TTTTGGCAAG GTGTCAAGTC GAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC 
TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA 
CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA 
CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT 
GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC 
ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG 
ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA 
ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG 
AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC 
TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT 
CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG 
ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT 
GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT 
CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG 
GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT 
CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC 
AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT 
GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG 
ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA 
GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT 
AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG 
AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG 
GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGG GCCAAACGAG 
GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC 
CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA 
TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA 
AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG 
GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT 
GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC 
CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC 
ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA 
GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA 
AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT 
GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG 
GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT 
TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC 
TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG 
ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC AACTCCCCAG AGCAGTACCA GGCAGCATTT 
CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT 
CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG 
GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGGAAACTTC 
ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC 
AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT 
ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT 
GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC 
AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA 
GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT 
TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG 
ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT 
GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG 
TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG 
GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG 
CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG 
GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA 
CGACATTACC AGACCCTCTG CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010796821.2
CDS	77..4603
Translation	MGQEPRTLPPSPNWYCSRCSDAVPGGLFGFAARTSVFLVRVGPGADAIPGTPPFRVVGELVGHTEKVSGFTFCHHPGQYNLCATSSDDGTVKIWDVGTKTVVTEHALHQHTISALHWSPRVKDLIVSGDEKGVVFCYWLNRNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVMHRLRGHDDEIHSIAWCPLLGEDCLSINQEENSEEPEMPNGKVITQTPVTKGCYLATGSKDQTIRIWSCSRGRGVMTLKLPFLKRRGGGVDPTVKERLWLTLHWPKDQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSGSSEGQNHSRIVFNLCPLQTEDDKQLLLSISMDRDVKCWDMATLECCWSLPSLGGFAYSLAFSPVDTGCLAIGVGDGMIRVWNTLSIKNNYDVKHFWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYSLAWGPPVPAMALGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFKLPNLKLICSIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLQTVIESNPQSPVTITEPYRTLSGHTAKITSLAWSPHHDGRLVSASYDGTAQVWDTLQEEPLCNFRGHRGRLLCVVWSPLDPDCIYSGADDFCVYKWLTSMQEHSRPPQGKKSIELEKKRLSQPKPKPKKKKKPTLRVPVRQELCDGNEEESVKESLGPIENGVSDQETEEEAREPEPPCGVTAAVSKEPIICTPAALGFEKSKSTINNKVTLLKKEVPKEKPEALIKKRKARSMLPLSTSLDHRSKEELHQDCLVLATAKHSTGPNEDVSTDLEERFHLGLFTDRATLYRMIEVEGKSHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPVAGYHVWVWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPIIKDLYLSWGAILEKDGHYALAAKCYLGATSAYDAAKVLAKKGDAASLRTAAELASIVGENELSASLALRCAQELLLARNWVGAQEALQLHTSLKGQRLVFCLLELLSKHLEEKQLSEGKSSFSSYNDWPTDSEGSFVERVTAVWKSTFGLNSPEQYQAAFQQLQNIKYPSATSNTPSKQLLLHICHDLTLAVLSQEAGSWDAAVEGLLRAVIRSYDSGNFTIMQEVHSAFLPQGCDHLRDKLGDHQSPTTPAFKSLKAFFIYGCLYEFWWSLSGPCPESSVWLRAAHRSKTSTEQSQEVDSVSPEETDPKPSQPEPSRTPELDVRLSEEGEQMQNACKELLSEKHAGLQNAQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEQEGQSLSHPQNQCKEEKNEPVSLPELTKRLTEANERIAKLPESIKTWPFPDVLECCLILLHIGSQCPSFVTQEMQQQAQEFLQKYGNTKVYKRHYQTLCT

Target ORF information:

RefSeq Version	XM_010798519.2
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010798519.2

ORF Insert Sequence:

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC 
TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA 
GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT 
GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA 
TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA 
TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA 
TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG 
TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG 
GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT 
TTTTGGCAAG GTGTCAAGTC GAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC 
TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA 
CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA 
CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT 
GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC 
ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG 
ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA 
ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG 
AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC 
TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT 
CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG 
ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT 
GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT 
CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG 
GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT 
CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC 
AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT 
GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG 
ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA 
GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT 
AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG 
AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG 
GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGG GCCAAACGAG 
GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC 
CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA 
TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA 
AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG 
GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT 
GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC 
CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC 
ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA 
GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA 
AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT 
GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG 
GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT 
TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC 
TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG 
ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC AACTCCCCAG AGCAGTACCA GGCAGCATTT 
CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT 
CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG 
GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGGAAACTTC 
ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC 
AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT 
ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT 
GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC 
AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA 
GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT 
TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG 
ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT 
GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG 
TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG 
GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG 
CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG 
GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA 
CGACATTACC AGACCCTCTG CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN5 ( XM_010807536.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805838.1 Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X2, mRNA.

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CloneID	OBa52457
Clone ID Related Accession (Same CDS sequence)	XM_010798519.2 , XM_010807536.2
Accession Version	XM_010807536.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	gem-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC 
TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA 
GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT 
GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA 
TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA 
TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA 
TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG 
TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG 
GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT 
TTTTGGCAAG GTGTCAAGTC GAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC 
TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA 
CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA 
CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT 
GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC 
ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG 
ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA 
ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG 
AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC 
TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT 
CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG 
ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT 
GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT 
CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG 
GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT 
CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC 
AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT 
GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG 
ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA 
GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT 
AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG 
AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG 
GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGG GCCAAACGAG 
GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC 
CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA 
TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA 
AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG 
GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT 
GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC 
CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC 
ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA 
GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA 
AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT 
GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG 
GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT 
TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC 
TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG 
ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC AACTCCCCAG AGCAGTACCA GGCAGCATTT 
CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT 
CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG 
GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGGAAACTTC 
ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC 
AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT 
ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT 
GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC 
AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA 
GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT 
TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG 
ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT 
GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG 
TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG 
GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG 
CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG 
GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA 
CGACATTACC AGACCCTCTG CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805838.1
CDS	77..4603
Translation	MGQEPRTLPPSPNWYCSRCSDAVPGGLFGFAARTSVFLVRVGPGADAIPGTPPFRVVGELVGHTEKVSGFTFCHHPGQYNLCATSSDDGTVKIWDVGTKTVVTEHALHQHTISALHWSPRVKDLIVSGDEKGVVFCYWLNRNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVMHRLRGHDDEIHSIAWCPLLGEDCLSINQEENSEEPEMPNGKVITQTPVTKGCYLATGSKDQTIRIWSCSRGRGVMTLKLPFLKRRGGGVDPTVKERLWLTLHWPKDQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSGSSEGQNHSRIVFNLCPLQTEDDKQLLLSISMDRDVKCWDMATLECCWSLPSLGGFAYSLAFSPVDTGCLAIGVGDGMIRVWNTLSIKNNYDVKHFWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYSLAWGPPVPAMALGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFKLPNLKLICSIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLQTVIESNPQSPVTITEPYRTLSGHTAKITSLAWSPHHDGRLVSASYDGTAQVWDTLQEEPLCNFRGHRGRLLCVVWSPLDPDCIYSGADDFCVYKWLTSMQEHSRPPQGKKSIELEKKRLSQPKPKPKKKKKPTLRVPVRQELCDGNEEESVKESLGPIENGVSDQETEEEAREPEPPCGVTAAVSKEPIICTPAALGFEKSKSTINNKVTLLKKEVPKEKPEALIKKRKARSMLPLSTSLDHRSKEELHQDCLVLATAKHSTGPNEDVSTDLEERFHLGLFTDRATLYRMIEVEGKSHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPVAGYHVWVWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPIIKDLYLSWGAILEKDGHYALAAKCYLGATSAYDAAKVLAKKGDAASLRTAAELASIVGENELSASLALRCAQELLLARNWVGAQEALQLHTSLKGQRLVFCLLELLSKHLEEKQLSEGKSSFSSYNDWPTDSEGSFVERVTAVWKSTFGLNSPEQYQAAFQQLQNIKYPSATSNTPSKQLLLHICHDLTLAVLSQEAGSWDAAVEGLLRAVIRSYDSGNFTIMQEVHSAFLPQGCDHLRDKLGDHQSPTTPAFKSLKAFFIYGCLYEFWWSLSGPCPESSVWLRAAHRSKTSTEQSQEVDSVSPEETDPKPSQPEPSRTPELDVRLSEEGEQMQNACKELLSEKHAGLQNAQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEQEGQSLSHPQNQCKEEKNEPVSLPELTKRLTEANERIAKLPESIKTWPFPDVLECCLILLHIGSQCPSFVTQEMQQQAQEFLQKYGNTKVYKRHYQTLCT

Target ORF information:

RefSeq Version	XM_010807536.2
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807536.2

ORF Insert Sequence:

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC 
TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA 
GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT 
GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA 
TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA 
TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA 
TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG 
TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG 
GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT 
TTTTGGCAAG GTGTCAAGTC GAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC 
TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA 
CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA 
CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT 
GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC 
ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG 
ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA 
ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG 
AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC 
TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT 
CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG 
ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT 
GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT 
CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG 
GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT 
CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC 
AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT 
GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG 
ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA 
GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT 
AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG 
AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG 
GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGG GCCAAACGAG 
GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC 
CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA 
TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA 
AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG 
GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT 
GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC 
CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC 
ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA 
GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA 
AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT 
GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG 
GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT 
TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC 
TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG 
ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC AACTCCCCAG AGCAGTACCA GGCAGCATTT 
CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT 
CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG 
GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGGAAACTTC 
ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC 
AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT 
ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT 
GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC 
AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA 
GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT 
TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG 
ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT 
GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG 
TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG 
GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG 
CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG 
GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA 
CGACATTACC AGACCCTCTG CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN5 ( XM_015464341.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319827.1 Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa52458
Clone ID Related Accession (Same CDS sequence)	XM_015464341.1 , XM_010807537.2
Accession Version	XM_015464341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	gem-associated protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC 
TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG 
GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT 
AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC 
AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT 
ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT 
CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT 
GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT 
GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT 
TGGCAAGGTG TCAAGTCGAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC 
TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT 
CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA 
GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA 
GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC 
AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC 
AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA 
TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT 
ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT 
GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT 
CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC 
ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT 
ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA 
GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA 
GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA 
GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA 
AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA 
GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT 
GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA 
CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC 
AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG 
CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG 
CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGCAGG GCCAAACGAG 
GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC 
CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA 
TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA 
AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG 
GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT 
GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC 
CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC 
ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA 
GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA 
AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT 
GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG 
GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT 
TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC 
TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG 
ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC AACTCCCCAG AGCAGTACCA GGCAGCATTT 
CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT 
CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG 
GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGGAAACTTC 
ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC 
AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT 
ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT 
GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC 
AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA 
GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT 
TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG 
ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT 
GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG 
TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG 
GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG 
CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG 
GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA 
CGACATTACC AGACCCTCTG CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319827.1
CDS	77..4603
Translation	MGQEPRTLPPSPNWYCSRCSDAVPGGLFGFAARTSVFLVRVGPGADAIPGTPPFRVVGELVGHTEKVSGFTFCHHPGQYNLCATSSDDGTVKIWDVGTKTVVTEHALHQHTISALHWSPRVKDLIVSGDEKGVVFCYWLNRNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVMHRLRGHDDEIHSIAWCPLLGEDCLSINQEENSEPEMPNGKVITQTPVTKGCYLATGSKDQTIRIWSCSRGRGVMTLKLPFLKRRGGGVDPTVKERLWLTLHWPKDQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSGSSEGQNHSRIVFNLCPLQTEDDKQLLLSISMDRDVKCWDMATLECCWSLPSLGGFAYSLAFSPVDTGCLAIGVGDGMIRVWNTLSIKNNYDVKHFWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYSLAWGPPVPAMALGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFKLPNLKLICSIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLQTVIESNPQSPVTITEPYRTLSGHTAKITSLAWSPHHDGRLVSASYDGTAQVWDTLQEEPLCNFRGHRGRLLCVVWSPLDPDCIYSGADDFCVYKWLTSMQEHSRPPQGKKSIELEKKRLSQPKPKPKKKKKPTLRVPVRQELCDGNEEESVKESLGPIENGVSDQETEEEAREPEPPCGVTAAVSKEPIICTPAALGFEKSKSTINNKVTLLKKEVPKEKPEALIKKRKARSMLPLSTSLDHRSKEELHQDCLVLATAKHSTAGPNEDVSTDLEERFHLGLFTDRATLYRMIEVEGKSHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPVAGYHVWVWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPIIKDLYLSWGAILEKDGHYALAAKCYLGATSAYDAAKVLAKKGDAASLRTAAELASIVGENELSASLALRCAQELLLARNWVGAQEALQLHTSLKGQRLVFCLLELLSKHLEEKQLSEGKSSFSSYNDWPTDSEGSFVERVTAVWKSTFGLNSPEQYQAAFQQLQNIKYPSATSNTPSKQLLLHICHDLTLAVLSQEAGSWDAAVEGLLRAVIRSYDSGNFTIMQEVHSAFLPQGCDHLRDKLGDHQSPTTPAFKSLKAFFIYGCLYEFWWSLSGPCPESSVWLRAAHRSKTSTEQSQEVDSVSPEETDPKPSQPEPSRTPELDVRLSEEGEQMQNACKELLSEKHAGLQNAQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEQEGQSLSHPQNQCKEEKNEPVSLPELTKRLTEANERIAKLPESIKTWPFPDVLECCLILLHIGSQCPSFVTQEMQQQAQEFLQKYGNTKVYKRHYQTLCT

Target ORF information:

RefSeq Version	XM_015464341.1
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464341.1

ORF Insert Sequence:

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC 
TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG 
GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT 
AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC 
AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT 
ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT 
CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT 
GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT 
GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT 
TGGCAAGGTG TCAAGTCGAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC 
TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT 
CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA 
GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA 
GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC 
AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC 
AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA 
TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT 
ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT 
GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT 
CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC 
ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT 
ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA 
GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA 
GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA 
GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA 
AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA 
GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT 
GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA 
CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC 
AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG 
CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG 
CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGCAGG GCCAAACGAG 
GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC 
CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA 
TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA 
AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG 
GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT 
GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC 
CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC 
ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA 
GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA 
AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT 
GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG 
GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT 
TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC 
TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG 
ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC AACTCCCCAG AGCAGTACCA GGCAGCATTT 
CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT 
CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG 
GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGGAAACTTC 
ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC 
AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT 
ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT 
GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC 
AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA 
GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT 
TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG 
ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT 
GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG 
TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG 
GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG 
CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG 
GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA 
CGACATTACC AGACCCTCTG CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN5 ( XM_010807537.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805839.1 Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa52458
Clone ID Related Accession (Same CDS sequence)	XM_015464341.1 , XM_010807537.2
Accession Version	XM_010807537.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	gem-associated protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC 
TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG 
GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT 
AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC 
AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT 
ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT 
CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT 
GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT 
GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT 
TGGCAAGGTG TCAAGTCGAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC 
TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT 
CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA 
GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA 
GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC 
AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC 
AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA 
TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT 
ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT 
GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT 
CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC 
ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT 
ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA 
GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA 
GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA 
GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA 
AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA 
GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT 
GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA 
CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC 
AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG 
CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG 
CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGCAGG GCCAAACGAG 
GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC 
CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA 
TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA 
AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG 
GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT 
GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC 
CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC 
ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA 
GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA 
AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT 
GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG 
GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT 
TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC 
TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG 
ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC AACTCCCCAG AGCAGTACCA GGCAGCATTT 
CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT 
CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG 
GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGGAAACTTC 
ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC 
AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT 
ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT 
GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC 
AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA 
GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT 
TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG 
ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT 
GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG 
TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG 
GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG 
CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG 
GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA 
CGACATTACC AGACCCTCTG CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805839.1
CDS	77..4603
Translation	MGQEPRTLPPSPNWYCSRCSDAVPGGLFGFAARTSVFLVRVGPGADAIPGTPPFRVVGELVGHTEKVSGFTFCHHPGQYNLCATSSDDGTVKIWDVGTKTVVTEHALHQHTISALHWSPRVKDLIVSGDEKGVVFCYWLNRNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVMHRLRGHDDEIHSIAWCPLLGEDCLSINQEENSEPEMPNGKVITQTPVTKGCYLATGSKDQTIRIWSCSRGRGVMTLKLPFLKRRGGGVDPTVKERLWLTLHWPKDQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSGSSEGQNHSRIVFNLCPLQTEDDKQLLLSISMDRDVKCWDMATLECCWSLPSLGGFAYSLAFSPVDTGCLAIGVGDGMIRVWNTLSIKNNYDVKHFWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYSLAWGPPVPAMALGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFKLPNLKLICSIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLQTVIESNPQSPVTITEPYRTLSGHTAKITSLAWSPHHDGRLVSASYDGTAQVWDTLQEEPLCNFRGHRGRLLCVVWSPLDPDCIYSGADDFCVYKWLTSMQEHSRPPQGKKSIELEKKRLSQPKPKPKKKKKPTLRVPVRQELCDGNEEESVKESLGPIENGVSDQETEEEAREPEPPCGVTAAVSKEPIICTPAALGFEKSKSTINNKVTLLKKEVPKEKPEALIKKRKARSMLPLSTSLDHRSKEELHQDCLVLATAKHSTAGPNEDVSTDLEERFHLGLFTDRATLYRMIEVEGKSHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPVAGYHVWVWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPIIKDLYLSWGAILEKDGHYALAAKCYLGATSAYDAAKVLAKKGDAASLRTAAELASIVGENELSASLALRCAQELLLARNWVGAQEALQLHTSLKGQRLVFCLLELLSKHLEEKQLSEGKSSFSSYNDWPTDSEGSFVERVTAVWKSTFGLNSPEQYQAAFQQLQNIKYPSATSNTPSKQLLLHICHDLTLAVLSQEAGSWDAAVEGLLRAVIRSYDSGNFTIMQEVHSAFLPQGCDHLRDKLGDHQSPTTPAFKSLKAFFIYGCLYEFWWSLSGPCPESSVWLRAAHRSKTSTEQSQEVDSVSPEETDPKPSQPEPSRTPELDVRLSEEGEQMQNACKELLSEKHAGLQNAQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEQEGQSLSHPQNQCKEEKNEPVSLPELTKRLTEANERIAKLPESIKTWPFPDVLECCLILLHIGSQCPSFVTQEMQQQAQEFLQKYGNTKVYKRHYQTLCT

Target ORF information:

RefSeq Version	XM_010807537.2
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807537.2

ORF Insert Sequence:

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC 
TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG 
GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT 
AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC 
AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT 
ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT 
CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT 
GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT 
GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT 
TGGCAAGGTG TCAAGTCGAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC 
TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT 
CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA 
GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA 
GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC 
AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC 
AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA 
TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT 
ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT 
GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT 
CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC 
ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT 
ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA 
GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA 
GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA 
GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA 
AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA 
GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT 
GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA 
CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC 
AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG 
CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG 
CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGCAGG GCCAAACGAG 
GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC 
CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA 
TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA 
AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG 
GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT 
GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC 
CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC 
ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA 
GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA 
AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT 
GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG 
GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT 
TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC 
TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG 
ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC AACTCCCCAG AGCAGTACCA GGCAGCATTT 
CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT 
CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG 
GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGGAAACTTC 
ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC 
AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT 
ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT 
GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC 
AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA 
GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT 
TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG 
ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT 
GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG 
TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG 
GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG 
CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG 
GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA 
CGACATTACC AGACCCTCTG CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN5 ( XM_015464342.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319828.1 Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X4, mRNA.

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CloneID	OBa52459
Clone ID Related Accession (Same CDS sequence)	XM_015464342.1 , XM_010807538.2
Accession Version	XM_015464342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	gem-associated protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC 
TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG 
GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT 
AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC 
AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT 
ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT 
CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT 
GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT 
GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT 
TGGCAAGGTG TCAAGTCGAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC 
TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT 
CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA 
GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA 
GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC 
AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC 
AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA 
TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT 
ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT 
GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT 
CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC 
ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT 
ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA 
GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA 
GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA 
GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA 
AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA 
GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT 
GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA 
CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC 
AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG 
CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG 
CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGGGCC AAACGAGGAT 
GTGTCTACTG ATCTTGAGGA AAGATTTCAC CTGGGGCTTT TCACAGACAG GGCTACCCTG 
TACAGAATGA TTGAGGTTGA AGGAAAAAGT CACTTGGAAA ATGGCCACCC CGAGTTATTT 
CACCAGCTCA TGCTTTGGAA AGGAGATCTA AAAGGTGTTC TGCAGACTGC AGCAGAAAGA 
GGGGAGCTGA CAGACAATCT CGTGGCTATG GCACCAGTCG CCGGTTACCA TGTGTGGGTA 
TGGGCTGTAG AAGCTTTTGC CAAACAACTG TGTTTTCAGG ACCAGTATGT CAAGGCTGCC 
TCTCACCTGC TTTCTATCCA CAAAGTGTAT GAAGCTGTGG AGCTGCTCAA GTCAAACCAT 
TTCTACAGGG AAGCTATTGC AATTGCTAAG GCCCGGCTGC GCCCAGAGGA CCCAATCATA 
AAAGACCTGT ACCTCAGCTG GGGAGCCATC CTAGAAAAAG ACGGCCACTA TGCCTTAGCA 
GCAAAATGCT ATTTAGGGGC CACTTCAGCT TATGATGCAG CCAAAGTTCT GGCCAAAAAG 
GGGGATGCGG CATCACTTAG GACGGCAGCA GAGTTGGCCT CCATCGTGGG AGAGAATGAG 
TTGTCAGCTT CCCTGGCTCT AAGATGTGCC CAAGAACTGC TTCTGGCCCG GAACTGGGTG 
GGAGCTCAGG AGGCCCTGCA GCTCCACACA AGTCTAAAGG GTCAGAGATT GGTGTTTTGC 
CTTCTTGAGC TACTGTCCAA GCACCTGGAG GAAAAGCAGC TCTCTGAGGG CAAAAGCTCC 
TTTTCTTCTT ACAATGATTG GCCCACAGAC AGCGAAGGGT CCTTTGTGGA GAGGGTGACT 
GCAGTATGGA AGAGCACCTT CGGCCTCAAC TCCCCAGAGC AGTACCAGGC AGCATTTCAG 
CAGCTGCAAA ATATCAAGTA CCCATCTGCT ACAAGTAACA CTCCCTCCAA ACAGCTTCTG 
CTCCACATTT GCCACGACCT GACCTTGGCA GTGCTAAGCC AGGAAGCGGG CTCCTGGGAC 
GCAGCTGTGG AAGGATTGCT TCGGGCTGTG ATCCGGAGCT ACGACTCAGG AAACTTCACC 
ATCATGCAGG AAGTGCACTC AGCCTTTCTT CCGCAGGGCT GTGACCATCT AAGAGACAAA 
CTGGGTGATC ATCAGTCTCC CACCACACCA GCTTTCAAAA GTCTGAAGGC CTTTTTTATT 
TACGGATGTC TATATGAATT CTGGTGGTCT CTCTCTGGGC CTTGCCCGGA GTCCAGTGTC 
TGGCTAAGAG CTGCTCACAG AAGTAAAACC TCTACTGAAC AGAGCCAAGA GGTTGACAGT 
GTCAGCCCTG AAGAAACTGA CCCCAAACCT TCCCAGCCAG AGCCAAGCAG GACTCCAGAA 
CTAGACGTGA GACTTTCAGA AGAAGGTGAA CAAATGCAGA ACGCTTGTAA GGAGCTTTTG 
TCAGAAAAGC ATGCTGGTCT CCAAAATGCA CAGAGAACTG TTGCTGAAGT CCAAGAGACC 
TTGGCAGAAA TGATCCGCCA ACACCAGAAG AGTCAACTGT GTAAATCCAC AGCAAATGGC 
CCTGATAAGA ATGAACCTGA ACAGGAAGGA CAGTCCCTCT CTCATCCTCA GAACCAGTGT 
AAAGAAGAAA AAAATGAACC AGTTTCTTTG CCTGAATTAA CCAAAAGACT TACAGAGGCA 
AATGAGAGAA TAGCTAAGCT TCCTGAGAGT ATTAAGACCT GGCCTTTCCC GGACGTGCTG 
GAATGCTGCC TCATCCTGCT TCACATTGGG TCCCAATGTC CTAGCTTTGT GACCCAGGAA 
ATGCAGCAAC AGGCTCAAGA GTTCCTTCAG AAGTACGGCA ACACAAAAGT TTACAAACGA 
CATTACCAGA CCCTCTGCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319828.1
CDS	77..4600
Translation	MGQEPRTLPPSPNWYCSRCSDAVPGGLFGFAARTSVFLVRVGPGADAIPGTPPFRVVGELVGHTEKVSGFTFCHHPGQYNLCATSSDDGTVKIWDVGTKTVVTEHALHQHTISALHWSPRVKDLIVSGDEKGVVFCYWLNRNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVMHRLRGHDDEIHSIAWCPLLGEDCLSINQEENSEPEMPNGKVITQTPVTKGCYLATGSKDQTIRIWSCSRGRGVMTLKLPFLKRRGGGVDPTVKERLWLTLHWPKDQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSGSSEGQNHSRIVFNLCPLQTEDDKQLLLSISMDRDVKCWDMATLECCWSLPSLGGFAYSLAFSPVDTGCLAIGVGDGMIRVWNTLSIKNNYDVKHFWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYSLAWGPPVPAMALGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFKLPNLKLICSIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLQTVIESNPQSPVTITEPYRTLSGHTAKITSLAWSPHHDGRLVSASYDGTAQVWDTLQEEPLCNFRGHRGRLLCVVWSPLDPDCIYSGADDFCVYKWLTSMQEHSRPPQGKKSIELEKKRLSQPKPKPKKKKKPTLRVPVRQELCDGNEEESVKESLGPIENGVSDQETEEEAREPEPPCGVTAAVSKEPIICTPAALGFEKSKSTINNKVTLLKKEVPKEKPEALIKKRKARSMLPLSTSLDHRSKEELHQDCLVLATAKHSTGPNEDVSTDLEERFHLGLFTDRATLYRMIEVEGKSHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPVAGYHVWVWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPIIKDLYLSWGAILEKDGHYALAAKCYLGATSAYDAAKVLAKKGDAASLRTAAELASIVGENELSASLALRCAQELLLARNWVGAQEALQLHTSLKGQRLVFCLLELLSKHLEEKQLSEGKSSFSSYNDWPTDSEGSFVERVTAVWKSTFGLNSPEQYQAAFQQLQNIKYPSATSNTPSKQLLLHICHDLTLAVLSQEAGSWDAAVEGLLRAVIRSYDSGNFTIMQEVHSAFLPQGCDHLRDKLGDHQSPTTPAFKSLKAFFIYGCLYEFWWSLSGPCPESSVWLRAAHRSKTSTEQSQEVDSVSPEETDPKPSQPEPSRTPELDVRLSEEGEQMQNACKELLSEKHAGLQNAQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEQEGQSLSHPQNQCKEEKNEPVSLPELTKRLTEANERIAKLPESIKTWPFPDVLECCLILLHIGSQCPSFVTQEMQQQAQEFLQKYGNTKVYKRHYQTLCT

Target ORF information:

RefSeq Version	XM_015464342.1
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464342.1

ORF Insert Sequence:

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC 
TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG 
GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT 
AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC 
AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT 
ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT 
CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT 
GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT 
GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT 
TGGCAAGGTG TCAAGTCGAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC 
TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT 
CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA 
GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA 
GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC 
AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC 
AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA 
TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT 
ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT 
GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT 
CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC 
ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT 
ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA 
GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA 
GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA 
GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA 
AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA 
GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT 
GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA 
CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC 
AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG 
CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG 
CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGGGCC AAACGAGGAT 
GTGTCTACTG ATCTTGAGGA AAGATTTCAC CTGGGGCTTT TCACAGACAG GGCTACCCTG 
TACAGAATGA TTGAGGTTGA AGGAAAAAGT CACTTGGAAA ATGGCCACCC CGAGTTATTT 
CACCAGCTCA TGCTTTGGAA AGGAGATCTA AAAGGTGTTC TGCAGACTGC AGCAGAAAGA 
GGGGAGCTGA CAGACAATCT CGTGGCTATG GCACCAGTCG CCGGTTACCA TGTGTGGGTA 
TGGGCTGTAG AAGCTTTTGC CAAACAACTG TGTTTTCAGG ACCAGTATGT CAAGGCTGCC 
TCTCACCTGC TTTCTATCCA CAAAGTGTAT GAAGCTGTGG AGCTGCTCAA GTCAAACCAT 
TTCTACAGGG AAGCTATTGC AATTGCTAAG GCCCGGCTGC GCCCAGAGGA CCCAATCATA 
AAAGACCTGT ACCTCAGCTG GGGAGCCATC CTAGAAAAAG ACGGCCACTA TGCCTTAGCA 
GCAAAATGCT ATTTAGGGGC CACTTCAGCT TATGATGCAG CCAAAGTTCT GGCCAAAAAG 
GGGGATGCGG CATCACTTAG GACGGCAGCA GAGTTGGCCT CCATCGTGGG AGAGAATGAG 
TTGTCAGCTT CCCTGGCTCT AAGATGTGCC CAAGAACTGC TTCTGGCCCG GAACTGGGTG 
GGAGCTCAGG AGGCCCTGCA GCTCCACACA AGTCTAAAGG GTCAGAGATT GGTGTTTTGC 
CTTCTTGAGC TACTGTCCAA GCACCTGGAG GAAAAGCAGC TCTCTGAGGG CAAAAGCTCC 
TTTTCTTCTT ACAATGATTG GCCCACAGAC AGCGAAGGGT CCTTTGTGGA GAGGGTGACT 
GCAGTATGGA AGAGCACCTT CGGCCTCAAC TCCCCAGAGC AGTACCAGGC AGCATTTCAG 
CAGCTGCAAA ATATCAAGTA CCCATCTGCT ACAAGTAACA CTCCCTCCAA ACAGCTTCTG 
CTCCACATTT GCCACGACCT GACCTTGGCA GTGCTAAGCC AGGAAGCGGG CTCCTGGGAC 
GCAGCTGTGG AAGGATTGCT TCGGGCTGTG ATCCGGAGCT ACGACTCAGG AAACTTCACC 
ATCATGCAGG AAGTGCACTC AGCCTTTCTT CCGCAGGGCT GTGACCATCT AAGAGACAAA 
CTGGGTGATC ATCAGTCTCC CACCACACCA GCTTTCAAAA GTCTGAAGGC CTTTTTTATT 
TACGGATGTC TATATGAATT CTGGTGGTCT CTCTCTGGGC CTTGCCCGGA GTCCAGTGTC 
TGGCTAAGAG CTGCTCACAG AAGTAAAACC TCTACTGAAC AGAGCCAAGA GGTTGACAGT 
GTCAGCCCTG AAGAAACTGA CCCCAAACCT TCCCAGCCAG AGCCAAGCAG GACTCCAGAA 
CTAGACGTGA GACTTTCAGA AGAAGGTGAA CAAATGCAGA ACGCTTGTAA GGAGCTTTTG 
TCAGAAAAGC ATGCTGGTCT CCAAAATGCA CAGAGAACTG TTGCTGAAGT CCAAGAGACC 
TTGGCAGAAA TGATCCGCCA ACACCAGAAG AGTCAACTGT GTAAATCCAC AGCAAATGGC 
CCTGATAAGA ATGAACCTGA ACAGGAAGGA CAGTCCCTCT CTCATCCTCA GAACCAGTGT 
AAAGAAGAAA AAAATGAACC AGTTTCTTTG CCTGAATTAA CCAAAAGACT TACAGAGGCA 
AATGAGAGAA TAGCTAAGCT TCCTGAGAGT ATTAAGACCT GGCCTTTCCC GGACGTGCTG 
GAATGCTGCC TCATCCTGCT TCACATTGGG TCCCAATGTC CTAGCTTTGT GACCCAGGAA 
ATGCAGCAAC AGGCTCAAGA GTTCCTTCAG AAGTACGGCA ACACAAAAGT TTACAAACGA 
CATTACCAGA CCCTCTGCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN5 ( XM_010807538.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805840.1 Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X4, mRNA.

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CloneID	OBa52459
Clone ID Related Accession (Same CDS sequence)	XM_015464342.1 , XM_010807538.2
Accession Version	XM_010807538.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	gem-associated protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807538.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC 
TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG 
GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT 
AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC 
AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT 
ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT 
CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT 
GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT 
GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT 
TGGCAAGGTG TCAAGTCGAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC 
TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT 
CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA 
GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA 
GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC 
AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC 
AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA 
TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT 
ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT 
GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT 
CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC 
ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT 
ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA 
GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA 
GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA 
GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA 
AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA 
GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT 
GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA 
CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC 
AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG 
CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG 
CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGGGCC AAACGAGGAT 
GTGTCTACTG ATCTTGAGGA AAGATTTCAC CTGGGGCTTT TCACAGACAG GGCTACCCTG 
TACAGAATGA TTGAGGTTGA AGGAAAAAGT CACTTGGAAA ATGGCCACCC CGAGTTATTT 
CACCAGCTCA TGCTTTGGAA AGGAGATCTA AAAGGTGTTC TGCAGACTGC AGCAGAAAGA 
GGGGAGCTGA CAGACAATCT CGTGGCTATG GCACCAGTCG CCGGTTACCA TGTGTGGGTA 
TGGGCTGTAG AAGCTTTTGC CAAACAACTG TGTTTTCAGG ACCAGTATGT CAAGGCTGCC 
TCTCACCTGC TTTCTATCCA CAAAGTGTAT GAAGCTGTGG AGCTGCTCAA GTCAAACCAT 
TTCTACAGGG AAGCTATTGC AATTGCTAAG GCCCGGCTGC GCCCAGAGGA CCCAATCATA 
AAAGACCTGT ACCTCAGCTG GGGAGCCATC CTAGAAAAAG ACGGCCACTA TGCCTTAGCA 
GCAAAATGCT ATTTAGGGGC CACTTCAGCT TATGATGCAG CCAAAGTTCT GGCCAAAAAG 
GGGGATGCGG CATCACTTAG GACGGCAGCA GAGTTGGCCT CCATCGTGGG AGAGAATGAG 
TTGTCAGCTT CCCTGGCTCT AAGATGTGCC CAAGAACTGC TTCTGGCCCG GAACTGGGTG 
GGAGCTCAGG AGGCCCTGCA GCTCCACACA AGTCTAAAGG GTCAGAGATT GGTGTTTTGC 
CTTCTTGAGC TACTGTCCAA GCACCTGGAG GAAAAGCAGC TCTCTGAGGG CAAAAGCTCC 
TTTTCTTCTT ACAATGATTG GCCCACAGAC AGCGAAGGGT CCTTTGTGGA GAGGGTGACT 
GCAGTATGGA AGAGCACCTT CGGCCTCAAC TCCCCAGAGC AGTACCAGGC AGCATTTCAG 
CAGCTGCAAA ATATCAAGTA CCCATCTGCT ACAAGTAACA CTCCCTCCAA ACAGCTTCTG 
CTCCACATTT GCCACGACCT GACCTTGGCA GTGCTAAGCC AGGAAGCGGG CTCCTGGGAC 
GCAGCTGTGG AAGGATTGCT TCGGGCTGTG ATCCGGAGCT ACGACTCAGG AAACTTCACC 
ATCATGCAGG AAGTGCACTC AGCCTTTCTT CCGCAGGGCT GTGACCATCT AAGAGACAAA 
CTGGGTGATC ATCAGTCTCC CACCACACCA GCTTTCAAAA GTCTGAAGGC CTTTTTTATT 
TACGGATGTC TATATGAATT CTGGTGGTCT CTCTCTGGGC CTTGCCCGGA GTCCAGTGTC 
TGGCTAAGAG CTGCTCACAG AAGTAAAACC TCTACTGAAC AGAGCCAAGA GGTTGACAGT 
GTCAGCCCTG AAGAAACTGA CCCCAAACCT TCCCAGCCAG AGCCAAGCAG GACTCCAGAA 
CTAGACGTGA GACTTTCAGA AGAAGGTGAA CAAATGCAGA ACGCTTGTAA GGAGCTTTTG 
TCAGAAAAGC ATGCTGGTCT CCAAAATGCA CAGAGAACTG TTGCTGAAGT CCAAGAGACC 
TTGGCAGAAA TGATCCGCCA ACACCAGAAG AGTCAACTGT GTAAATCCAC AGCAAATGGC 
CCTGATAAGA ATGAACCTGA ACAGGAAGGA CAGTCCCTCT CTCATCCTCA GAACCAGTGT 
AAAGAAGAAA AAAATGAACC AGTTTCTTTG CCTGAATTAA CCAAAAGACT TACAGAGGCA 
AATGAGAGAA TAGCTAAGCT TCCTGAGAGT ATTAAGACCT GGCCTTTCCC GGACGTGCTG 
GAATGCTGCC TCATCCTGCT TCACATTGGG TCCCAATGTC CTAGCTTTGT GACCCAGGAA 
ATGCAGCAAC AGGCTCAAGA GTTCCTTCAG AAGTACGGCA ACACAAAAGT TTACAAACGA 
CATTACCAGA CCCTCTGCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805840.1
CDS	77..4600
Translation	MGQEPRTLPPSPNWYCSRCSDAVPGGLFGFAARTSVFLVRVGPGADAIPGTPPFRVVGELVGHTEKVSGFTFCHHPGQYNLCATSSDDGTVKIWDVGTKTVVTEHALHQHTISALHWSPRVKDLIVSGDEKGVVFCYWLNRNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVMHRLRGHDDEIHSIAWCPLLGEDCLSINQEENSEPEMPNGKVITQTPVTKGCYLATGSKDQTIRIWSCSRGRGVMTLKLPFLKRRGGGVDPTVKERLWLTLHWPKDQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSGSSEGQNHSRIVFNLCPLQTEDDKQLLLSISMDRDVKCWDMATLECCWSLPSLGGFAYSLAFSPVDTGCLAIGVGDGMIRVWNTLSIKNNYDVKHFWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYSLAWGPPVPAMALGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFKLPNLKLICSIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLQTVIESNPQSPVTITEPYRTLSGHTAKITSLAWSPHHDGRLVSASYDGTAQVWDTLQEEPLCNFRGHRGRLLCVVWSPLDPDCIYSGADDFCVYKWLTSMQEHSRPPQGKKSIELEKKRLSQPKPKPKKKKKPTLRVPVRQELCDGNEEESVKESLGPIENGVSDQETEEEAREPEPPCGVTAAVSKEPIICTPAALGFEKSKSTINNKVTLLKKEVPKEKPEALIKKRKARSMLPLSTSLDHRSKEELHQDCLVLATAKHSTGPNEDVSTDLEERFHLGLFTDRATLYRMIEVEGKSHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPVAGYHVWVWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPIIKDLYLSWGAILEKDGHYALAAKCYLGATSAYDAAKVLAKKGDAASLRTAAELASIVGENELSASLALRCAQELLLARNWVGAQEALQLHTSLKGQRLVFCLLELLSKHLEEKQLSEGKSSFSSYNDWPTDSEGSFVERVTAVWKSTFGLNSPEQYQAAFQQLQNIKYPSATSNTPSKQLLLHICHDLTLAVLSQEAGSWDAAVEGLLRAVIRSYDSGNFTIMQEVHSAFLPQGCDHLRDKLGDHQSPTTPAFKSLKAFFIYGCLYEFWWSLSGPCPESSVWLRAAHRSKTSTEQSQEVDSVSPEETDPKPSQPEPSRTPELDVRLSEEGEQMQNACKELLSEKHAGLQNAQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEQEGQSLSHPQNQCKEEKNEPVSLPELTKRLTEANERIAKLPESIKTWPFPDVLECCLILLHIGSQCPSFVTQEMQQQAQEFLQKYGNTKVYKRHYQTLCT

Target ORF information:

RefSeq Version	XM_010807538.2
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807538.2

ORF Insert Sequence:

ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC 
GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG 
GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC 
CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA 
GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA 
GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC 
AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG 
CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC 
ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC 
ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA 
GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC 
TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG 
GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT 
AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC 
AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT 
ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT 
CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT 
GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT 
GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT 
TGGCAAGGTG TCAAGTCGAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC 
TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT 
CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA 
GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA 
GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC 
AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC 
AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA 
TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT 
ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT 
GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT 
CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC 
ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT 
ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA 
GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA 
GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA 
GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA 
AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA 
GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT 
GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA 
CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC 
AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG 
CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG 
CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGGGCC AAACGAGGAT 
GTGTCTACTG ATCTTGAGGA AAGATTTCAC CTGGGGCTTT TCACAGACAG GGCTACCCTG 
TACAGAATGA TTGAGGTTGA AGGAAAAAGT CACTTGGAAA ATGGCCACCC CGAGTTATTT 
CACCAGCTCA TGCTTTGGAA AGGAGATCTA AAAGGTGTTC TGCAGACTGC AGCAGAAAGA 
GGGGAGCTGA CAGACAATCT CGTGGCTATG GCACCAGTCG CCGGTTACCA TGTGTGGGTA 
TGGGCTGTAG AAGCTTTTGC CAAACAACTG TGTTTTCAGG ACCAGTATGT CAAGGCTGCC 
TCTCACCTGC TTTCTATCCA CAAAGTGTAT GAAGCTGTGG AGCTGCTCAA GTCAAACCAT 
TTCTACAGGG AAGCTATTGC AATTGCTAAG GCCCGGCTGC GCCCAGAGGA CCCAATCATA 
AAAGACCTGT ACCTCAGCTG GGGAGCCATC CTAGAAAAAG ACGGCCACTA TGCCTTAGCA 
GCAAAATGCT ATTTAGGGGC CACTTCAGCT TATGATGCAG CCAAAGTTCT GGCCAAAAAG 
GGGGATGCGG CATCACTTAG GACGGCAGCA GAGTTGGCCT CCATCGTGGG AGAGAATGAG 
TTGTCAGCTT CCCTGGCTCT AAGATGTGCC CAAGAACTGC TTCTGGCCCG GAACTGGGTG 
GGAGCTCAGG AGGCCCTGCA GCTCCACACA AGTCTAAAGG GTCAGAGATT GGTGTTTTGC 
CTTCTTGAGC TACTGTCCAA GCACCTGGAG GAAAAGCAGC TCTCTGAGGG CAAAAGCTCC 
TTTTCTTCTT ACAATGATTG GCCCACAGAC AGCGAAGGGT CCTTTGTGGA GAGGGTGACT 
GCAGTATGGA AGAGCACCTT CGGCCTCAAC TCCCCAGAGC AGTACCAGGC AGCATTTCAG 
CAGCTGCAAA ATATCAAGTA CCCATCTGCT ACAAGTAACA CTCCCTCCAA ACAGCTTCTG 
CTCCACATTT GCCACGACCT GACCTTGGCA GTGCTAAGCC AGGAAGCGGG CTCCTGGGAC 
GCAGCTGTGG AAGGATTGCT TCGGGCTGTG ATCCGGAGCT ACGACTCAGG AAACTTCACC 
ATCATGCAGG AAGTGCACTC AGCCTTTCTT CCGCAGGGCT GTGACCATCT AAGAGACAAA 
CTGGGTGATC ATCAGTCTCC CACCACACCA GCTTTCAAAA GTCTGAAGGC CTTTTTTATT 
TACGGATGTC TATATGAATT CTGGTGGTCT CTCTCTGGGC CTTGCCCGGA GTCCAGTGTC 
TGGCTAAGAG CTGCTCACAG AAGTAAAACC TCTACTGAAC AGAGCCAAGA GGTTGACAGT 
GTCAGCCCTG AAGAAACTGA CCCCAAACCT TCCCAGCCAG AGCCAAGCAG GACTCCAGAA 
CTAGACGTGA GACTTTCAGA AGAAGGTGAA CAAATGCAGA ACGCTTGTAA GGAGCTTTTG 
TCAGAAAAGC ATGCTGGTCT CCAAAATGCA CAGAGAACTG TTGCTGAAGT CCAAGAGACC 
TTGGCAGAAA TGATCCGCCA ACACCAGAAG AGTCAACTGT GTAAATCCAC AGCAAATGGC 
CCTGATAAGA ATGAACCTGA ACAGGAAGGA CAGTCCCTCT CTCATCCTCA GAACCAGTGT 
AAAGAAGAAA AAAATGAACC AGTTTCTTTG CCTGAATTAA CCAAAAGACT TACAGAGGCA 
AATGAGAGAA TAGCTAAGCT TCCTGAGAGT ATTAAGACCT GGCCTTTCCC GGACGTGCTG 
GAATGCTGCC TCATCCTGCT TCACATTGGG TCCCAATGTC CTAGCTTTGT GACCCAGGAA 
ATGCAGCAAC AGGCTCAAGA GTTCCTTCAG AAGTACGGCA ACACAAAAGT TTACAAACGA 
CATTACCAGA CCCTCTGCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GEMIN5
Entrez Gene ID	533444
Full Name	gem nuclear organelle associated protein 5
General protein information	Preferred Names gem nuclear organelle associated protein 5 Names gem-associated protein 5
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	GEMIN5	XP_001112869.2
Danio rerio (zebrafish)	gemin5	XP_001339880.4
Homo sapiens (human)	GEMIN5	NP_056280.2
Xenopus tropicalis (tropical clawed frog)	gemin5	XP_004912844.1
Bos taurus (cattle)	GEMIN5	XP_003586382.1
Canis lupus familiaris (dog)	GEMIN5	XP_005619319.1
Mus musculus (house mouse)	Gemin5	NP_001160141.1
Gallus gallus (chicken)	GEMIN5	XP_414574.3
Pan troglodytes (chimpanzee)	GEMIN5	XP_527093.2
Rattus norvegicus (Norway rat)	Gemin5	NP_001165560.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

GEMIN5 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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