gemin5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	gemin5
Entrez Gene ID	100493267
Full Name	gem nuclear organelle associated protein 5
General protein information	Preferred Names gem nuclear organelle associated protein 5 Names gem-associated protein 5
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004912787.1	XP_004912844.1	gem-associated protein 5 isoform X1
XM_031898704.1	XP_031754564.1	gem-associated protein 5 isoform X3
XM_018092816.1	XP_017948305.1	gem-associated protein 5 isoform X3
XM_004912786.2	XP_004912843.1	gem-associated protein 5 isoform X1
XM_004912786.1	XP_004912843.1	gem-associated protein 5 isoform X1
XM_004912787.2	XP_004912844.1	gem-associated protein 5 isoform X1
XM_002932376.2	XP_002932422.1	gem-associated protein 5 isoform X2
XM_031898703.1	XP_031754563.1	gem-associated protein 5 isoform X2

Pathways from BioSystems

Homologies

	GEMIN5	XP_001112869.2
Danio rerio (zebrafish)	gemin5	XP_001339880.4
Homo sapiens (human)	GEMIN5	NP_056280.2
Xenopus tropicalis (tropical clawed frog)	gemin5	XP_004912844.1
Bos taurus (cattle)	GEMIN5	XP_003586382.1
Canis lupus familiaris (dog)	GEMIN5	XP_005619319.1
Mus musculus (house mouse)	Gemin5	NP_001160141.1
Gallus gallus (chicken)	GEMIN5	XP_414574.3
Pan troglodytes (chimpanzee)	GEMIN5	XP_527093.2
Rattus norvegicus (Norway rat)	Gemin5	NP_001165560.1

Gene Ontology

The following gemin5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gemin5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa43670	XM_018092816.1 Latest version!	Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OXa54342	XM_004912786.2 Latest version!	Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa54342	XM_004912787.2 Latest version!	Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa54343	XM_031898703.1 Latest version!	Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa54344	XM_031898704.1 Latest version!	Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa07510	XM_004912786.1 Latest version!	Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa07510	XM_004912787.1 Latest version!	Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa07511	XM_002932376.2 Latest version!	Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gemin5 ( XM_018092816.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948305.1 Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa43670
Clone ID Related Accession (Same CDS sequence)	XM_018092816.1
Accession Version	XM_018092816.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gem-associated protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAC CAGATCGGAT GCTCAGTGCG TCACCCAACT GGTACTGTAG CCGCTCCAGT 
GATGCTGCCG GTGGACTGTT CGGATTCGCT GCTCGAAATT CTGTCTACCT CCTGAACATT 
AATCTGCAGG AGCCTGGATT TTATGGAGAG CTGGTTGGAC ATACAGACCG CGTCTCTGGC 
TTTGCTTTTT CCCATTTCTC AGGCCAATCT CACCTGTGTG CCAGTAGCTC TGATGATGGT 
ACTGTAAAGG TTTGGGACAC GCAGACAAAG AACACTGTAA CGGAGCACCA TCTCCATCGG 
AGTACAATTG CTGCTCTTCA CTGGTCACCT TTAGTGAAGG ATCTGCTGGT ATCCGGGGAT 
GAAAAAGGAG TATTGGTTTG TTTCTGGTGT AACCGAAATG ACAGCCAGCA GTTTTTTCCA 
GAGCCAAGGA CTATTTTCTG CCTTTCCTGC TCCCCGCACA AGGAGGACAT TGTAGCTATA 
GGGTACAAAG ATGGCATGAT AGTGCTGGTG GATATCAGCC GCAGAGGAGA TGTGTTGCAT 
CGGCTACGAG GGCATGAAGA GGAGATACAC TGTTTGGAAT GGAGCCCCCA TGCCTTGGAG 
TGTGACCTGC AACAAAAGCT GGAGGAGACA GAGATAAACA CAGCCAATGG GGAGCTTCTT 
CCTGAGACAG AAAACAGGAG ATGTTATCTG GCTTCTGGCA GCAAAGACCA GACTGTCCGT 
ATCTGGAGTG CTTCTACAGG CAAAGCAGTT ACAGTGTTGA AGCTGCCATA CTTAAAAAGA 
CGTGGAGGCG GAGTGGATCC AGCCATCAAG GAGCGGCTCT GGTTGACACT GCACTGGCCA 
CCTGGCCATT CAACACAGAT TCTGTCCAGT TGTTTTGGGG GTGAATTGCT CCTGTGGGAT 
TTAACCAAAT CTGGGAAGCA GAAGTGGACT CTCCTTGGCA CCTCTGATGG ACAGAATCAT 
TCTCGAATTG TCTTTAACCT TTGTTCTGTG GTCACTGAGG AAGGGAAAGA GCTCTTGCTG 
TCTACTTCCA TGGACCGAGA TGTGAAATGT TGGGATCTGG GTTCCCTGTC CTGCTGCTGG 
TCCCTAACTT CCCTCGGTGG CTTTGTATAC AGCCTAGCTT TCTCCCCGGT AGCAGCTGGC 
AGCCTGGCTG TTGGGGTGGG GGACAGCATG ATCCGTGTAT GGAATACAAT GTCGGTTGTC 
AATGCATATG ATGTGAAGAC CCTGTGGCAG GGCATCAGAT CCAAGGTCAC TGCGCTGTCA 
TGGCATCCGA CCAAAGAGGG CAGTCTTGCT TTTGGCACCG AGGATGGGAA AGTTGGCATC 
TTTGAGGTCT ACATCAGCAA ACCCCCTCAG ATTTCTGCTA CATACCACAA AAAGACTGTT 
TATTCCTTAT CATGGGGACC TCTGCTGCCT CCATTCTCAT TTGGAGCAGA AGGGGACCGT 
CCTACTTATA CACTGTACAG CTGTGGGGGG GAAGGGGTTA TTTACCAGCA CAATCCTTGG 
AAACTGACAG ATGAAGCCAG TAATATTAAC AAGCTGATCC GGGACACCAA TGACATCAAA 
CACAAGTTAC CAGCACACAC AGAGTTAAGC TGGAGACCTG ATGGACAGTA TTTAGCTATT 
GGCAATGAAG ATGGAACCAT TGAGGTCTTC CACACTCAGC GTCTGCAGCT TATCTGTACC 
ATTCAGCAGC ATCACAAGCT AGTGAATGCT GTGCGCTGGC ATCATGGCCA TAGCCAAGAC 
CCTGACTTGA ACTTTCTGCT GGCATCTGGA TCTAACAATG CTACTGTCTA CGTGCACAAC 
CTGAAAGGGC CCCTGGAGAA TCCCACAGAT TCCCCAGTCA CCATCACTGA GCCTTTCCGT 
ACCTTGCCTG GACACACTGC AAAGATCACA AGCCTGTCAT GGAGCCCTCA CCACGATGCC 
AGACTGGTGT CTGCCAGTTA TGATGGTACT GCCCAGGTAT GGGATGTTCT GCAGGAAGAG 
CCACTCAGTA ACTATCGTGG GCACAAAGGA AGACTGCTTT CTGTTCAGTG GTCTCCTGTG 
GAGCCAGACC AGGTGTGGAC AGGAGCTGAT GACTTTTGCC TTCATAGCTG GTCTGTATCA 
AAGCAAGAGC ATAGCAGGCC TCCCAAAGGG AAAAAAGCCA CTGAGCTTGA AAAAAAGCGT 
ACCCTTCAAC CAAAACCAAA AGCCAAGAAG AAGAAAAAGT TAGACACCCC CAAAGAGGAG 
GCAGGAAGAG AGGTACAGAG GGGAGAAAAG GAAAGCCCTC TAACACAGAA TGGGACATCC 
GATCAAGAGG AGGGACAGGG GACTCCTGAG CCAGAGGCAG CTGACAGAGG CTTACATGAA 
GATCTGCAAA TCAGGAAGAA GAAGAGTCGA TCGTTGCTTC CCATCAGCAC CAGCAAAGAT 
CACCGATCTA AAGAAGAGCA ACACCGAGAC TGTGTTAAGT TGGCAACTGT TGTATGTGCG 
AAGGATTTGG AAGAGAAATC TCATTTTCAC TCGGAGGACA CAATGCACCT GGGACTATAC 
AGTGACCGAG CAGCCCTTTA TCACCTTATG GAGGAGGAGG GTAAGGCTCA TCTGGAGAAT 
GGGCACCCTG AGCTCCAGCA TCAAATTATG CTTTGGAAAG GGGATCTGAA GGGGACTTTG 
CAACTGGCTG CAGAGAGGGG TGAACTGACT GATCAGCTGG TGGCGCTGTC TCCCGTAGCC 
GGATACAAGG TCTGGCTATG GACCGTTGAA GCCTTTGTGA AACAGCTGTG CTTTCATGAG 
CAGTACGTGA AAGCCGCTTC CCACCTGCTG TCCATTAATA AAGTGTATGA AGCCGTGGAA 
CTGCTCAAGG GGAACCACCT CTACAGAGAA GCCATTGCAG TGGCGAAGGC TCGGCTACTC 
CCCGATGATT CAGTCCTGCG GGAGCTGTAC ACTAGCTGGG CAGCAGTCCT TGAGCAGGAC 
GGGCACTATT CCATGGCTGC TAAGTGTTAT TTAGGGTCCT CCTCTCCCTA TGATGCTGCC 
AAGGTTCTAG CTAAGAAGGG GGATTTGCTA TCCCTGAGCG CTGCTGCTGA ACTTGCATTA 
GTTGCAGGAG AAAAAGACCT TGCTGCTTCT TTTGCCATCC GCTGCGCCCA GGAACTGTTC 
ACTGCAAAGA ATTTTTTAGC AGCCCAGGAA GCACTACGAC AGCACAACAG TCTGCTGGGG 
CAGAGACTGC TGTTTTGTAC CAGTGAGCTG CTGTACATGC ATTTGAAGGA GAAGATGCCC 
ATAGATTGGA AGACACAAAG CAATCCATGT TTCCATGAAC AGACAGGCAG TAGTAATGGG 
GAACCTTTTA TGACCAAGCT TACAAGTCTA TGGCAGAGTT TGTGTGAAGT AAACAGCCCA 
GAGCAATACA AAGATGCCTT GCAACAACTG TCTGCCTCAG AGATTCCACA GACCACTGTC 
AATACTGGCC CTAAACAGCT GCTGCTGCAC CTTTCCCATG AGGTCACAAT ATTGTCTCTG 
AATTGTCAGC TTAAAAACTG GAAAGAGGCT GTTCCATCTC TCCTCCATAT CCTGTTCCGC 
TGTCAGCAGG CAGGCCATTT CACCCTGATG CAAGGGATAG CCAGCCTGCT GCTTCCTAAC 
GGCTGTGAAG AGCTGAGGGC TCTGTTGTCC CCAGAAAACA AAGAGAATTT GACTGCCTGT 
GACAGCGTGC AGGCCTTCCT TGCTTACATA AAGATGTATG AGCTGTGGTG GGGACAGCAA 
GAAATGCCAC CAAATTCCAA AGATCCAGCG GCTGCAGTAA ATATGGACAG TCCAAATGGA 
GCCTCTGCTG CTGTGCAGGA GCCAGATATG AAACCAGGGG ATTTGTGCGG CTTTCAGGTG 
TTGCTGTCAG ACCTTCACGC GCCCTTCCAG AGTGCCCAAA GAGAGATTGC TGAGGTTCAG 
AGAGCCCTTC GGGAGATGAT ACAGCAACAC CAGAGAATCA GGCAGAACCA GGACAGCTCT 
TTGGAACTAG AACAAGAGCC TGAATTAAAC ACTTCTTCAT CTCAGGATAA ATCTCTTGAC 
AGCCAAGTCA GCATATCCGC TGGTCCAAGT CCTGTGTCAC TGACAGAGCT GATGAAGAAG 
CTGTCAGTGG CCAACCACCG ACTAGCAGAG TTCCCTGAAT ATTTAAAGGC CTTACCCTTT 
CCTGATGTAC TAGAGAGCTG CCTGGTGCTA CTGCATCTTC CATCCTATCA GATCTCCCCA 
GAGATCCGCT CCCAAGCCTT GGCGTTGTTG CACAGTCATA GCCATGGATC TGCTGATCAC 
CAAATAGCAG CCAAGAAGTT CCTCTCCATC CCAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948305.1
CDS	99..4397
Translation	MGEPDRMLSASPNWYCSRSSDAAGGLFGFAARNSVYLLNINLQEPGFYGELVGHTDRVSGFAFSHFSGQSHLCASSSDDGTVKVWDTQTKNTVTEHHLHRSTIAALHWSPLVKDLLVSGDEKGVLVCFWCNRNDSQQFFPEPRTIFCLSCSPHKEDIVAIGYKDGMIVLVDISRRGDVLHRLRGHEEEIHCLEWSPHALECDLQQKLEETEINTANGELLPETENRRCYLASGSKDQTVRIWSASTGKAVTVLKLPYLKRRGGGVDPAIKERLWLTLHWPPGHSTQILSSCFGGELLLWDLTKSGKQKWTLLGTSDGQNHSRIVFNLCSVVTEEGKELLLSTSMDRDVKCWDLGSLSCCWSLTSLGGFVYSLAFSPVAAGSLAVGVGDSMIRVWNTMSVVNAYDVKTLWQGIRSKVTALSWHPTKEGSLAFGTEDGKVGIFEVYISKPPQISATYHKKTVYSLSWGPLLPPFSFGAEGDRPTYTLYSCGGEGVIYQHNPWKLTDEASNINKLIRDTNDIKHKLPAHTELSWRPDGQYLAIGNEDGTIEVFHTQRLQLICTIQQHHKLVNAVRWHHGHSQDPDLNFLLASGSNNATVYVHNLKGPLENPTDSPVTITEPFRTLPGHTAKITSLSWSPHHDARLVSASYDGTAQVWDVLQEEPLSNYRGHKGRLLSVQWSPVEPDQVWTGADDFCLHSWSVSKQEHSRPPKGKKATELEKKRTLQPKPKAKKKKKLDTPKEEAGREVQRGEKESPLTQNGTSDQEEGQGTPEPEAADRGLHEDLQIRKKKSRSLLPISTSKDHRSKEEQHRDCVKLATVVCAKDLEEKSHFHSEDTMHLGLYSDRAALYHLMEEEGKAHLENGHPELQHQIMLWKGDLKGTLQLAAERGELTDQLVALSPVAGYKVWLWTVEAFVKQLCFHEQYVKAASHLLSINKVYEAVELLKGNHLYREAIAVAKARLLPDDSVLRELYTSWAAVLEQDGHYSMAAKCYLGSSSPYDAAKVLAKKGDLLSLSAAAELALVAGEKDLAASFAIRCAQELFTAKNFLAAQEALRQHNSLLGQRLLFCTSELLYMHLKEKMPIDWKTQSNPCFHEQTGSSNGEPFMTKLTSLWQSLCEVNSPEQYKDALQQLSASEIPQTTVNTGPKQLLLHLSHEVTILSLNCQLKNWKEAVPSLLHILFRCQQAGHFTLMQGIASLLLPNGCEELRALLSPENKENLTACDSVQAFLAYIKMYELWWGQQEMPPNSKDPAAAVNMDSPNGASAAVQEPDMKPGDLCGFQVLLSDLHAPFQSAQREIAEVQRALREMIQQHQRIRQNQDSSLELEQEPELNTSSSQDKSLDSQVSISAGPSPVSLTELMKKLSVANHRLAEFPEYLKALPFPDVLESCLVLLHLPSYQISPEIRSQALALLHSHSHGSADHQIAAKKFLSIPE

Target ORF information:

RefSeq Version	XM_018092816.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092816.1

ORF Insert Sequence:

ATGGGGGAAC CAGATCGGAT GCTCAGTGCG TCACCCAACT GGTACTGTAG CCGCTCCAGT 
GATGCTGCCG GTGGACTGTT CGGATTCGCT GCTCGAAATT CTGTCTACCT CCTGAACATT 
AATCTGCAGG AGCCTGGATT TTATGGAGAG CTGGTTGGAC ATACAGACCG CGTCTCTGGC 
TTTGCTTTTT CCCATTTCTC AGGCCAATCT CACCTGTGTG CCAGTAGCTC TGATGATGGT 
ACTGTAAAGG TTTGGGACAC GCAGACAAAG AACACTGTAA CGGAGCACCA TCTCCATCGG 
AGTACAATTG CTGCTCTTCA CTGGTCACCT TTAGTGAAGG ATCTGCTGGT ATCCGGGGAT 
GAAAAAGGAG TATTGGTTTG TTTCTGGTGT AACCGAAATG ACAGCCAGCA GTTTTTTCCA 
GAGCCAAGGA CTATTTTCTG CCTTTCCTGC TCCCCGCACA AGGAGGACAT TGTAGCTATA 
GGGTACAAAG ATGGCATGAT AGTGCTGGTG GATATCAGCC GCAGAGGAGA TGTGTTGCAT 
CGGCTACGAG GGCATGAAGA GGAGATACAC TGTTTGGAAT GGAGCCCCCA TGCCTTGGAG 
TGTGACCTGC AACAAAAGCT GGAGGAGACA GAGATAAACA CAGCCAATGG GGAGCTTCTT 
CCTGAGACAG AAAACAGGAG ATGTTATCTG GCTTCTGGCA GCAAAGACCA GACTGTCCGT 
ATCTGGAGTG CTTCTACAGG CAAAGCAGTT ACAGTGTTGA AGCTGCCATA CTTAAAAAGA 
CGTGGAGGCG GAGTGGATCC AGCCATCAAG GAGCGGCTCT GGTTGACACT GCACTGGCCA 
CCTGGCCATT CAACACAGAT TCTGTCCAGT TGTTTTGGGG GTGAATTGCT CCTGTGGGAT 
TTAACCAAAT CTGGGAAGCA GAAGTGGACT CTCCTTGGCA CCTCTGATGG ACAGAATCAT 
TCTCGAATTG TCTTTAACCT TTGTTCTGTG GTCACTGAGG AAGGGAAAGA GCTCTTGCTG 
TCTACTTCCA TGGACCGAGA TGTGAAATGT TGGGATCTGG GTTCCCTGTC CTGCTGCTGG 
TCCCTAACTT CCCTCGGTGG CTTTGTATAC AGCCTAGCTT TCTCCCCGGT AGCAGCTGGC 
AGCCTGGCTG TTGGGGTGGG GGACAGCATG ATCCGTGTAT GGAATACAAT GTCGGTTGTC 
AATGCATATG ATGTGAAGAC CCTGTGGCAG GGCATCAGAT CCAAGGTCAC TGCGCTGTCA 
TGGCATCCGA CCAAAGAGGG CAGTCTTGCT TTTGGCACCG AGGATGGGAA AGTTGGCATC 
TTTGAGGTCT ACATCAGCAA ACCCCCTCAG ATTTCTGCTA CATACCACAA AAAGACTGTT 
TATTCCTTAT CATGGGGACC TCTGCTGCCT CCATTCTCAT TTGGAGCAGA AGGGGACCGT 
CCTACTTATA CACTGTACAG CTGTGGGGGG GAAGGGGTTA TTTACCAGCA CAATCCTTGG 
AAACTGACAG ATGAAGCCAG TAATATTAAC AAGCTGATCC GGGACACCAA TGACATCAAA 
CACAAGTTAC CAGCACACAC AGAGTTAAGC TGGAGACCTG ATGGACAGTA TTTAGCTATT 
GGCAATGAAG ATGGAACCAT TGAGGTCTTC CACACTCAGC GTCTGCAGCT TATCTGTACC 
ATTCAGCAGC ATCACAAGCT AGTGAATGCT GTGCGCTGGC ATCATGGCCA TAGCCAAGAC 
CCTGACTTGA ACTTTCTGCT GGCATCTGGA TCTAACAATG CTACTGTCTA CGTGCACAAC 
CTGAAAGGGC CCCTGGAGAA TCCCACAGAT TCCCCAGTCA CCATCACTGA GCCTTTCCGT 
ACCTTGCCTG GACACACTGC AAAGATCACA AGCCTGTCAT GGAGCCCTCA CCACGATGCC 
AGACTGGTGT CTGCCAGTTA TGATGGTACT GCCCAGGTAT GGGATGTTCT GCAGGAAGAG 
CCACTCAGTA ACTATCGTGG GCACAAAGGA AGACTGCTTT CTGTTCAGTG GTCTCCTGTG 
GAGCCAGACC AGGTGTGGAC AGGAGCTGAT GACTTTTGCC TTCATAGCTG GTCTGTATCA 
AAGCAAGAGC ATAGCAGGCC TCCCAAAGGG AAAAAAGCCA CTGAGCTTGA AAAAAAGCGT 
ACCCTTCAAC CAAAACCAAA AGCCAAGAAG AAGAAAAAGT TAGACACCCC CAAAGAGGAG 
GCAGGAAGAG AGGTACAGAG GGGAGAAAAG GAAAGCCCTC TAACACAGAA TGGGACATCC 
GATCAAGAGG AGGGACAGGG GACTCCTGAG CCAGAGGCAG CTGACAGAGG CTTACATGAA 
GATCTGCAAA TCAGGAAGAA GAAGAGTCGA TCGTTGCTTC CCATCAGCAC CAGCAAAGAT 
CACCGATCTA AAGAAGAGCA ACACCGAGAC TGTGTTAAGT TGGCAACTGT TGTATGTGCG 
AAGGATTTGG AAGAGAAATC TCATTTTCAC TCGGAGGACA CAATGCACCT GGGACTATAC 
AGTGACCGAG CAGCCCTTTA TCACCTTATG GAGGAGGAGG GTAAGGCTCA TCTGGAGAAT 
GGGCACCCTG AGCTCCAGCA TCAAATTATG CTTTGGAAAG GGGATCTGAA GGGGACTTTG 
CAACTGGCTG CAGAGAGGGG TGAACTGACT GATCAGCTGG TGGCGCTGTC TCCCGTAGCC 
GGATACAAGG TCTGGCTATG GACCGTTGAA GCCTTTGTGA AACAGCTGTG CTTTCATGAG 
CAGTACGTGA AAGCCGCTTC CCACCTGCTG TCCATTAATA AAGTGTATGA AGCCGTGGAA 
CTGCTCAAGG GGAACCACCT CTACAGAGAA GCCATTGCAG TGGCGAAGGC TCGGCTACTC 
CCCGATGATT CAGTCCTGCG GGAGCTGTAC ACTAGCTGGG CAGCAGTCCT TGAGCAGGAC 
GGGCACTATT CCATGGCTGC TAAGTGTTAT TTAGGGTCCT CCTCTCCCTA TGATGCTGCC 
AAGGTTCTAG CTAAGAAGGG GGATTTGCTA TCCCTGAGCG CTGCTGCTGA ACTTGCATTA 
GTTGCAGGAG AAAAAGACCT TGCTGCTTCT TTTGCCATCC GCTGCGCCCA GGAACTGTTC 
ACTGCAAAGA ATTTTTTAGC AGCCCAGGAA GCACTACGAC AGCACAACAG TCTGCTGGGG 
CAGAGACTGC TGTTTTGTAC CAGTGAGCTG CTGTACATGC ATTTGAAGGA GAAGATGCCC 
ATAGATTGGA AGACACAAAG CAATCCATGT TTCCATGAAC AGACAGGCAG TAGTAATGGG 
GAACCTTTTA TGACCAAGCT TACAAGTCTA TGGCAGAGTT TGTGTGAAGT AAACAGCCCA 
GAGCAATACA AAGATGCCTT GCAACAACTG TCTGCCTCAG AGATTCCACA GACCACTGTC 
AATACTGGCC CTAAACAGCT GCTGCTGCAC CTTTCCCATG AGGTCACAAT ATTGTCTCTG 
AATTGTCAGC TTAAAAACTG GAAAGAGGCT GTTCCATCTC TCCTCCATAT CCTGTTCCGC 
TGTCAGCAGG CAGGCCATTT CACCCTGATG CAAGGGATAG CCAGCCTGCT GCTTCCTAAC 
GGCTGTGAAG AGCTGAGGGC TCTGTTGTCC CCAGAAAACA AAGAGAATTT GACTGCCTGT 
GACAGCGTGC AGGCCTTCCT TGCTTACATA AAGATGTATG AGCTGTGGTG GGGACAGCAA 
GAAATGCCAC CAAATTCCAA AGATCCAGCG GCTGCAGTAA ATATGGACAG TCCAAATGGA 
GCCTCTGCTG CTGTGCAGGA GCCAGATATG AAACCAGGGG ATTTGTGCGG CTTTCAGGTG 
TTGCTGTCAG ACCTTCACGC GCCCTTCCAG AGTGCCCAAA GAGAGATTGC TGAGGTTCAG 
AGAGCCCTTC GGGAGATGAT ACAGCAACAC CAGAGAATCA GGCAGAACCA GGACAGCTCT 
TTGGAACTAG AACAAGAGCC TGAATTAAAC ACTTCTTCAT CTCAGGATAA ATCTCTTGAC 
AGCCAAGTCA GCATATCCGC TGGTCCAAGT CCTGTGTCAC TGACAGAGCT GATGAAGAAG 
CTGTCAGTGG CCAACCACCG ACTAGCAGAG TTCCCTGAAT ATTTAAAGGC CTTACCCTTT 
CCTGATGTAC TAGAGAGCTG CCTGGTGCTA CTGCATCTTC CATCCTATCA GATCTCCCCA 
GAGATCCGCT CCCAAGCCTT GGCGTTGTTG CACAGTCATA GCCATGGATC TGCTGATCAC 
CAAATAGCAG CCAAGAAGTT CCTCTCCATC CCAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gemin5 ( XM_004912786.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912843.1 Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa54342
Clone ID Related Accession (Same CDS sequence)	XM_004912786.2 , XM_004912787.2
Accession Version	XM_004912786.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gem-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912786.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAC CAGATCGGAT GCTCAGTGCG TCACCCAACT GGTACTGTAG CCGCTCCAGT 
GATGCTGCCG GTGGACTGTT CGGATTCGCT GCTCGAAATT CTGTCTACCT CCTGAACATT 
AATCTGCAGG AGCCTGGATT TTATGGAGAG CTGGTTGGAC ATACAGACCG CGTCTCTGGC 
TTTGCTTTTT CCCATTTCTC AGGCCAATCT CACCTGTGTG CCAGTAGCTC TGATGATGGT 
ACTGTAAAGG TTTGGGACAC GCAGACAAAG AACACTGTAA CGGAGCACCA TCTCCATCGG 
AGTACAATTG CTGCTCTTCA CTGGTCACCT TTAGTGAAGG ATCTGCTGGT ATCCGGGGAT 
GAAAAAGGAG TATTGGTTTG TTTCTGGTGT AACCGAAATG ACAGCCAGCA GTTTTTTCCA 
GAGCCAAGGA CTATTTTCTG CCTTTCCTGC TCCCCGCACA AGGAGGACAT TGTAGCTATA 
GGGTACAAAG ATGGCATGAT AGTGCTGGTG GATATCAGCC GCAGAGGAGA TGTGTTGCAT 
CGGCTACGAG GGCATGAAGA GGAGATACAC TGTTTGGAAT GGAGCCCCCA TGCCTTGGAG 
TGTGACCTGC AACAAAAGCT GGAGGAGACA GAGATAAACA CAGCCAATGG GGAGCTTCTT 
CCTGAGACAG AAAACAGGAG ATGTTATCTG GCTTCTGGCA GCAAAGACCA GACTGTCCGT 
ATCTGGAGTG CTTCTACAGG CAAAGCAGTT ACAGTGTTGA AGCTGCCATA CTTAAAAAGA 
CGTGGAGGCG GAGTGGATCC AGCCATCAAG GAGCGGCTCT GGTTGACACT GCACTGGCCA 
CCTGGCCATT CAACACAGAT TCTGTCCAGT TGTTTTGGGG GTGAATTGCT CCTGTGGGAT 
TTAACCAAAT CTGGGAAGCA GAAGTGGACT CTCCTTGGCA CCTCTGATGG ACAGAATCAT 
TCTCGAATTG TCTTTAACCT TTGTTCTGTG GTCACTGAGG AAGGGAAAGA GCTCTTGCTG 
TCTACTTCCA TGGACCGAGA TGTGAAATGT TGGGATCTGG GTTCCCTGTC CTGCTGCTGG 
TCCCTAACTT CCCTCGGTGG CTTTGTATAC AGCCTAGCTT TCTCCCCGGT AGCAGCTGGC 
AGCCTGGCTG TTGGGGTGGG GGACAGCATG ATCCGTGTAT GGAATACAAT GTCGGTTGTC 
AATGCATATG ATGTGAAGAC CCTGTGGCAG GGCATCAGAT CCAAGGTCAC TGCGCTGTCA 
TGGCATCCGA CCAAAGAGGG CAGTCTTGCT TTTGGCACCG AGGATGGGAA AGTTGGCATC 
TTTGAGGTCT ACATCAGCAA ACCCCCTCAG ATTTCTGCTA CATACCACAA AAAGACTGTT 
TATTCCTTAT CATGGGGACC TCTGCTGCCT CCATTCTCAT TTGGAGCAGA AGGGGACCGT 
CCTACTTATA CACTGTACAG CTGTGGGGGG GAAGGGGTTA TTTACCAGCA CAATCCTTGG 
AAACTGACAG ATGAAGCCAG TAATATTAAC AAGCTGATCC GGGACACCAA TGACATCAAA 
CACAAGTTAC CAGCACACAC AGAGTTAAGC TGGAGACCTG ATGGACAGTA TTTAGCTATT 
GGCAATGAAG ATGGAACCAT TGAGGTCTTC CACACTCAGC GTCTGCAGCT TATCTGTACC 
ATTCAGCAGC ATCACAAGCT AGTGAATGCT GTGCGCTGGC ATCATGGCCA TAGCCAAGAC 
CCTGACTTGA ACTTTCTGCT GGCATCTGGA TCTAACAATG CTACTGTCTA CGTGCACAAC 
CTGAAAGGGC CCCTGGAGAA TCCAACAGAT TCCCCAGTCA CCATCACTGA GCCTTTCCGT 
ACCTTGCCTG GACACACTGC AAAGATCACA AGCCTGTCAT GGAGCCCTCA CCACGATGCC 
AGACTGGTGT CTGCCAGTTA TGATGGTACT GCCCAGGTAT GGGATGTTCT GCAGGAAGAG 
CCACTCAGTA ACTATCGTGG GCACAAAGGA AGACTGCTTT CTGTTCAGTG GTCTCCTGTG 
GAGCCAGACC AGGTGTGGAC AGGAGCTGAT GACTTTTGCC TTCATAGCTG GTCTGTATCA 
AAGCAAGAGC ATAGCAGGCC TCCCAAAGGG AAAAAAGCCA CTGAGCTTGA AAAAAAGCGT 
ACCCTTCAAC CAAAACCAAA AGCCAAGAAG AAGAAAAAGT TAGACACCCC CAAAGAGGAG 
GCAGGAAGAG AGGTACAGAG GGGAGAAAAG GAAAGCCCTC TAACACAGAA TGGGACATCC 
GATCAAGAGG AGGGACAGGG GACTCCTGAG CCAGAGGCAG CTGACAGAGG CTTACATGAA 
GCAGATGTAG AATGTCCTTT TCAGCCAGTG GTCCCTTCTC CAGTTCCTGC TCAGGACAAG 
CACAGTCCCA TTTCATTTGC TAAGACTGTA CCAGTTAGGA AGGACCTGGC CAAAGAACGA 
GCAGATCTGC AAATCAGGAA GAAGAAGAGT CGATCGTTGC TTCCCATCAG CACCAGCAAA 
GATCACCGAT CTAAAGAAGA GCAACACCGA GACTGTGTTA AGTTGGCAAC TGTTGTATGT 
GCGAAGGATT TGGAAGAGAA ATCTCATTTT CACTCGGAGG ACACAATGCA CCTGGGACTA 
TACAGTGACC GAGCAGCCCT TTATCACCTT ATGGAGGAGG AGGGTAAGGC TCATCTGGAG 
AATGGGCACC CTGAGCTCCA GCATCAAATT ATGCTTTGGA AAGGGGATCT GAAGGGGACT 
TTGCAACTGG CTGCAGAGAG GGGTGAACTG ACTGATCAGC TGGTGGCGCT GTCTCCCGTA 
GCCGGATACA AGGTCTGGCT ATGGACCGTT GAAGCCTTTG TGAAACAGCT GTGCTTTCAT 
GAGCAGTACG TGAAAGCCGC TTCCCACCTG CTGTCCATTA ATAAAGTGTA TGAAGCCGTG 
GAACTGCTCA AGGGGAACCA CCTCTACAGA GAAGCCATTG CAGTGGCGAA GGCTCGGCTA 
CTCCCCGATG ATTCAGTCCT GCGGGAGCTG TACACTAGCT GGGCAGCAGT CCTTGAGCAG 
GACGGGCACT ATTCCATGGC TGCTAAGTGT TATTTAGGGT CCTCCTCTCC CTATGATGCT 
GCCAAGGTTC TAGCTAAGAA GGGGGATTTG CTATCCCTGA GCGCTGCTGC TGAACTTGCA 
TTAGTTGCAG GAGAAAAAGA CCTTGCTGCT TCTTTTGCCA TCCGCTGCGC CCAGGAACTG 
TTCACTGCAA AGAATTTTTT AGCAGCCCAG GAAGCACTAC GACAGCACAA CAGTCTGCTG 
GGGCAGAGAC TGCTGTTTTG TACCAGTGAG CTGCTGTACA TGCATTTGAA GGAGAAGATG 
CCCATAGATT GGAAGACACA AAGCAATCCA TGTTTCCATG AACAGACAGG CAGTAGTAAT 
GGGGAACCTT TTATGACCAA GCTTACAAGT CTATGGCAGA GTTTGTGTGA AGTAAACAGC 
CCAGAGCAAT ACAAAGATGC CTTGCAACAA CTGTCTGCCT CAGAGATTCC ACAGACCACT 
GTCAATACTG GCCCTAAACA GCTGCTGCTG CACCTTTCCC ATGAGGTCAC AATATTGTCT 
CTGAATTGTC AGCTTAAAAA CTGGAAAGAG GCTGTTCCAT CTCTCCTCCA TATCCTGTTC 
CGCTGTCAGC AGGCAGGCCA TTTCACCCTG ATGCAAGGGA TAGCCAGCCT GCTGCTTCCT 
AACGGCTGTG AAGAGCTGAG GGCTCTGTTG TCCCCAGAAA ACAAAGAGAA TTTGACTGCC 
TGTGACAGCG TGCAGGCCTT CCTTGCTTAC ATAAAGATGT ATGAGCTGTG GTGGGGACAG 
CAAGAAATGC CACCAAATTC CAAAGATCCA GCGGCTGCAG TAAATATGGA CAGTCCAAAT 
GGAGCCTCTG CTGCTGTGCA GGAGCCAGAT ATGAAACCAG GGGATTTGTG CGGCTTTCAG 
GTGTTGCTGT CAGACCTTCA CGCGCCCTTC CAGAGTGCCC AAAGAGAGAT TGCTGAGGTT 
CAGAGAGCCC TTCGGGAGAT GATACAGCAA CACCAGAGAA TCAGGCAGAA CCAGGACAGC 
TCTTTGGAAC TAGAACAAGA GCCTGAATTA AACACTTCTT CATCTCAGGA TAAATCTCTT 
GACAGCCAAG TCAGCATATC CGCTGGTCCA AGTCCTGTGT CACTGACAGA GCTGATGAAG 
AAGCTGTCAG TGGCCAACCA CCGACTAGCA GAGTTCCCTG AATATTTAAA GGCCTTACCC 
TTTCCTGATG TACTAGAGAG CTGCCTGGTG CTACTGCATC TTCCATCCTA TCAGATCTCC 
CCAGAGATCC GCTCCCAAGC CTTGGCGTTG TTGCACAGTC ATAGCCATGG ATCTGCTGAT 
CACCAAATAG CAGCCAAGAA GTTCCTCTCC ATCCCAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912843.1
CDS	100..4521
Translation	MGEPDRMLSASPNWYCSRSSDAAGGLFGFAARNSVYLLNINLQEPGFYGELVGHTDRVSGFAFSHFSGQSHLCASSSDDGTVKVWDTQTKNTVTEHHLHRSTIAALHWSPLVKDLLVSGDEKGVLVCFWCNRNDSQQFFPEPRTIFCLSCSPHKEDIVAIGYKDGMIVLVDISRRGDVLHRLRGHEEEIHCLEWSPHALECDLQQKLEETEINTANGELLPETENRRCYLASGSKDQTVRIWSASTGKAVTVLKLPYLKRRGGGVDPAIKERLWLTLHWPPGHSTQILSSCFGGELLLWDLTKSGKQKWTLLGTSDGQNHSRIVFNLCSVVTEEGKELLLSTSMDRDVKCWDLGSLSCCWSLTSLGGFVYSLAFSPVAAGSLAVGVGDSMIRVWNTMSVVNAYDVKTLWQGIRSKVTALSWHPTKEGSLAFGTEDGKVGIFEVYISKPPQISATYHKKTVYSLSWGPLLPPFSFGAEGDRPTYTLYSCGGEGVIYQHNPWKLTDEASNINKLIRDTNDIKHKLPAHTELSWRPDGQYLAIGNEDGTIEVFHTQRLQLICTIQQHHKLVNAVRWHHGHSQDPDLNFLLASGSNNATVYVHNLKGPLENPTDSPVTITEPFRTLPGHTAKITSLSWSPHHDARLVSASYDGTAQVWDVLQEEPLSNYRGHKGRLLSVQWSPVEPDQVWTGADDFCLHSWSVSKQEHSRPPKGKKATELEKKRTLQPKPKAKKKKKLDTPKEEAGREVQRGEKESPLTQNGTSDQEEGQGTPEPEAADRGLHEADVECPFQPVVPSPVPAQDKHSPISFAKTVPVRKDLAKERADLQIRKKKSRSLLPISTSKDHRSKEEQHRDCVKLATVVCAKDLEEKSHFHSEDTMHLGLYSDRAALYHLMEEEGKAHLENGHPELQHQIMLWKGDLKGTLQLAAERGELTDQLVALSPVAGYKVWLWTVEAFVKQLCFHEQYVKAASHLLSINKVYEAVELLKGNHLYREAIAVAKARLLPDDSVLRELYTSWAAVLEQDGHYSMAAKCYLGSSSPYDAAKVLAKKGDLLSLSAAAELALVAGEKDLAASFAIRCAQELFTAKNFLAAQEALRQHNSLLGQRLLFCTSELLYMHLKEKMPIDWKTQSNPCFHEQTGSSNGEPFMTKLTSLWQSLCEVNSPEQYKDALQQLSASEIPQTTVNTGPKQLLLHLSHEVTILSLNCQLKNWKEAVPSLLHILFRCQQAGHFTLMQGIASLLLPNGCEELRALLSPENKENLTACDSVQAFLAYIKMYELWWGQQEMPPNSKDPAAAVNMDSPNGASAAVQEPDMKPGDLCGFQVLLSDLHAPFQSAQREIAEVQRALREMIQQHQRIRQNQDSSLELEQEPELNTSSSQDKSLDSQVSISAGPSPVSLTELMKKLSVANHRLAEFPEYLKALPFPDVLESCLVLLHLPSYQISPEIRSQALALLHSHSHGSADHQIAAKKFLSIPE

Target ORF information:

RefSeq Version	XM_004912786.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912786.2

ORF Insert Sequence:

ATGGGGGAAC CAGATCGGAT GCTCAGTGCG TCACCCAACT GGTACTGTAG CCGCTCCAGT 
GATGCTGCCG GTGGACTGTT CGGATTCGCT GCTCGAAATT CTGTCTACCT CCTGAACATT 
AATCTGCAGG AGCCTGGATT TTATGGAGAG CTGGTTGGAC ATACAGACCG CGTCTCTGGC 
TTTGCTTTTT CCCATTTCTC AGGCCAATCT CACCTGTGTG CCAGTAGCTC TGATGATGGT 
ACTGTAAAGG TTTGGGACAC GCAGACAAAG AACACTGTAA CGGAGCACCA TCTCCATCGG 
AGTACAATTG CTGCTCTTCA CTGGTCACCT TTAGTGAAGG ATCTGCTGGT ATCCGGGGAT 
GAAAAAGGAG TATTGGTTTG TTTCTGGTGT AACCGAAATG ACAGCCAGCA GTTTTTTCCA 
GAGCCAAGGA CTATTTTCTG CCTTTCCTGC TCCCCGCACA AGGAGGACAT TGTAGCTATA 
GGGTACAAAG ATGGCATGAT AGTGCTGGTG GATATCAGCC GCAGAGGAGA TGTGTTGCAT 
CGGCTACGAG GGCATGAAGA GGAGATACAC TGTTTGGAAT GGAGCCCCCA TGCCTTGGAG 
TGTGACCTGC AACAAAAGCT GGAGGAGACA GAGATAAACA CAGCCAATGG GGAGCTTCTT 
CCTGAGACAG AAAACAGGAG ATGTTATCTG GCTTCTGGCA GCAAAGACCA GACTGTCCGT 
ATCTGGAGTG CTTCTACAGG CAAAGCAGTT ACAGTGTTGA AGCTGCCATA CTTAAAAAGA 
CGTGGAGGCG GAGTGGATCC AGCCATCAAG GAGCGGCTCT GGTTGACACT GCACTGGCCA 
CCTGGCCATT CAACACAGAT TCTGTCCAGT TGTTTTGGGG GTGAATTGCT CCTGTGGGAT 
TTAACCAAAT CTGGGAAGCA GAAGTGGACT CTCCTTGGCA CCTCTGATGG ACAGAATCAT 
TCTCGAATTG TCTTTAACCT TTGTTCTGTG GTCACTGAGG AAGGGAAAGA GCTCTTGCTG 
TCTACTTCCA TGGACCGAGA TGTGAAATGT TGGGATCTGG GTTCCCTGTC CTGCTGCTGG 
TCCCTAACTT CCCTCGGTGG CTTTGTATAC AGCCTAGCTT TCTCCCCGGT AGCAGCTGGC 
AGCCTGGCTG TTGGGGTGGG GGACAGCATG ATCCGTGTAT GGAATACAAT GTCGGTTGTC 
AATGCATATG ATGTGAAGAC CCTGTGGCAG GGCATCAGAT CCAAGGTCAC TGCGCTGTCA 
TGGCATCCGA CCAAAGAGGG CAGTCTTGCT TTTGGCACCG AGGATGGGAA AGTTGGCATC 
TTTGAGGTCT ACATCAGCAA ACCCCCTCAG ATTTCTGCTA CATACCACAA AAAGACTGTT 
TATTCCTTAT CATGGGGACC TCTGCTGCCT CCATTCTCAT TTGGAGCAGA AGGGGACCGT 
CCTACTTATA CACTGTACAG CTGTGGGGGG GAAGGGGTTA TTTACCAGCA CAATCCTTGG 
AAACTGACAG ATGAAGCCAG TAATATTAAC AAGCTGATCC GGGACACCAA TGACATCAAA 
CACAAGTTAC CAGCACACAC AGAGTTAAGC TGGAGACCTG ATGGACAGTA TTTAGCTATT 
GGCAATGAAG ATGGAACCAT TGAGGTCTTC CACACTCAGC GTCTGCAGCT TATCTGTACC 
ATTCAGCAGC ATCACAAGCT AGTGAATGCT GTGCGCTGGC ATCATGGCCA TAGCCAAGAC 
CCTGACTTGA ACTTTCTGCT GGCATCTGGA TCTAACAATG CTACTGTCTA CGTGCACAAC 
CTGAAAGGGC CCCTGGAGAA TCCAACAGAT TCCCCAGTCA CCATCACTGA GCCTTTCCGT 
ACCTTGCCTG GACACACTGC AAAGATCACA AGCCTGTCAT GGAGCCCTCA CCACGATGCC 
AGACTGGTGT CTGCCAGTTA TGATGGTACT GCCCAGGTAT GGGATGTTCT GCAGGAAGAG 
CCACTCAGTA ACTATCGTGG GCACAAAGGA AGACTGCTTT CTGTTCAGTG GTCTCCTGTG 
GAGCCAGACC AGGTGTGGAC AGGAGCTGAT GACTTTTGCC TTCATAGCTG GTCTGTATCA 
AAGCAAGAGC ATAGCAGGCC TCCCAAAGGG AAAAAAGCCA CTGAGCTTGA AAAAAAGCGT 
ACCCTTCAAC CAAAACCAAA AGCCAAGAAG AAGAAAAAGT TAGACACCCC CAAAGAGGAG 
GCAGGAAGAG AGGTACAGAG GGGAGAAAAG GAAAGCCCTC TAACACAGAA TGGGACATCC 
GATCAAGAGG AGGGACAGGG GACTCCTGAG CCAGAGGCAG CTGACAGAGG CTTACATGAA 
GCAGATGTAG AATGTCCTTT TCAGCCAGTG GTCCCTTCTC CAGTTCCTGC TCAGGACAAG 
CACAGTCCCA TTTCATTTGC TAAGACTGTA CCAGTTAGGA AGGACCTGGC CAAAGAACGA 
GCAGATCTGC AAATCAGGAA GAAGAAGAGT CGATCGTTGC TTCCCATCAG CACCAGCAAA 
GATCACCGAT CTAAAGAAGA GCAACACCGA GACTGTGTTA AGTTGGCAAC TGTTGTATGT 
GCGAAGGATT TGGAAGAGAA ATCTCATTTT CACTCGGAGG ACACAATGCA CCTGGGACTA 
TACAGTGACC GAGCAGCCCT TTATCACCTT ATGGAGGAGG AGGGTAAGGC TCATCTGGAG 
AATGGGCACC CTGAGCTCCA GCATCAAATT ATGCTTTGGA AAGGGGATCT GAAGGGGACT 
TTGCAACTGG CTGCAGAGAG GGGTGAACTG ACTGATCAGC TGGTGGCGCT GTCTCCCGTA 
GCCGGATACA AGGTCTGGCT ATGGACCGTT GAAGCCTTTG TGAAACAGCT GTGCTTTCAT 
GAGCAGTACG TGAAAGCCGC TTCCCACCTG CTGTCCATTA ATAAAGTGTA TGAAGCCGTG 
GAACTGCTCA AGGGGAACCA CCTCTACAGA GAAGCCATTG CAGTGGCGAA GGCTCGGCTA 
CTCCCCGATG ATTCAGTCCT GCGGGAGCTG TACACTAGCT GGGCAGCAGT CCTTGAGCAG 
GACGGGCACT ATTCCATGGC TGCTAAGTGT TATTTAGGGT CCTCCTCTCC CTATGATGCT 
GCCAAGGTTC TAGCTAAGAA GGGGGATTTG CTATCCCTGA GCGCTGCTGC TGAACTTGCA 
TTAGTTGCAG GAGAAAAAGA CCTTGCTGCT TCTTTTGCCA TCCGCTGCGC CCAGGAACTG 
TTCACTGCAA AGAATTTTTT AGCAGCCCAG GAAGCACTAC GACAGCACAA CAGTCTGCTG 
GGGCAGAGAC TGCTGTTTTG TACCAGTGAG CTGCTGTACA TGCATTTGAA GGAGAAGATG 
CCCATAGATT GGAAGACACA AAGCAATCCA TGTTTCCATG AACAGACAGG CAGTAGTAAT 
GGGGAACCTT TTATGACCAA GCTTACAAGT CTATGGCAGA GTTTGTGTGA AGTAAACAGC 
CCAGAGCAAT ACAAAGATGC CTTGCAACAA CTGTCTGCCT CAGAGATTCC ACAGACCACT 
GTCAATACTG GCCCTAAACA GCTGCTGCTG CACCTTTCCC ATGAGGTCAC AATATTGTCT 
CTGAATTGTC AGCTTAAAAA CTGGAAAGAG GCTGTTCCAT CTCTCCTCCA TATCCTGTTC 
CGCTGTCAGC AGGCAGGCCA TTTCACCCTG ATGCAAGGGA TAGCCAGCCT GCTGCTTCCT 
AACGGCTGTG AAGAGCTGAG GGCTCTGTTG TCCCCAGAAA ACAAAGAGAA TTTGACTGCC 
TGTGACAGCG TGCAGGCCTT CCTTGCTTAC ATAAAGATGT ATGAGCTGTG GTGGGGACAG 
CAAGAAATGC CACCAAATTC CAAAGATCCA GCGGCTGCAG TAAATATGGA CAGTCCAAAT 
GGAGCCTCTG CTGCTGTGCA GGAGCCAGAT ATGAAACCAG GGGATTTGTG CGGCTTTCAG 
GTGTTGCTGT CAGACCTTCA CGCGCCCTTC CAGAGTGCCC AAAGAGAGAT TGCTGAGGTT 
CAGAGAGCCC TTCGGGAGAT GATACAGCAA CACCAGAGAA TCAGGCAGAA CCAGGACAGC 
TCTTTGGAAC TAGAACAAGA GCCTGAATTA AACACTTCTT CATCTCAGGA TAAATCTCTT 
GACAGCCAAG TCAGCATATC CGCTGGTCCA AGTCCTGTGT CACTGACAGA GCTGATGAAG 
AAGCTGTCAG TGGCCAACCA CCGACTAGCA GAGTTCCCTG AATATTTAAA GGCCTTACCC 
TTTCCTGATG TACTAGAGAG CTGCCTGGTG CTACTGCATC TTCCATCCTA TCAGATCTCC 
CCAGAGATCC GCTCCCAAGC CTTGGCGTTG TTGCACAGTC ATAGCCATGG ATCTGCTGAT 
CACCAAATAG CAGCCAAGAA GTTCCTCTCC ATCCCAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gemin5 ( XM_004912787.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912844.1 Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X1, mRNA.

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CloneID	OXa54342
Clone ID Related Accession (Same CDS sequence)	XM_004912786.2 , XM_004912787.2
Accession Version	XM_004912787.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gem-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912787.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAC CAGATCGGAT GCTCAGTGCG TCACCCAACT GGTACTGTAG CCGCTCCAGT 
GATGCTGCCG GTGGACTGTT CGGATTCGCT GCTCGAAATT CTGTCTACCT CCTGAACATT 
AATCTGCAGG AGCCTGGATT TTATGGAGAG CTGGTTGGAC ATACAGACCG CGTCTCTGGC 
TTTGCTTTTT CCCATTTCTC AGGCCAATCT CACCTGTGTG CCAGTAGCTC TGATGATGGT 
ACTGTAAAGG TTTGGGACAC GCAGACAAAG AACACTGTAA CGGAGCACCA TCTCCATCGG 
AGTACAATTG CTGCTCTTCA CTGGTCACCT TTAGTGAAGG ATCTGCTGGT ATCCGGGGAT 
GAAAAAGGAG TATTGGTTTG TTTCTGGTGT AACCGAAATG ACAGCCAGCA GTTTTTTCCA 
GAGCCAAGGA CTATTTTCTG CCTTTCCTGC TCCCCGCACA AGGAGGACAT TGTAGCTATA 
GGGTACAAAG ATGGCATGAT AGTGCTGGTG GATATCAGCC GCAGAGGAGA TGTGTTGCAT 
CGGCTACGAG GGCATGAAGA GGAGATACAC TGTTTGGAAT GGAGCCCCCA TGCCTTGGAG 
TGTGACCTGC AACAAAAGCT GGAGGAGACA GAGATAAACA CAGCCAATGG GGAGCTTCTT 
CCTGAGACAG AAAACAGGAG ATGTTATCTG GCTTCTGGCA GCAAAGACCA GACTGTCCGT 
ATCTGGAGTG CTTCTACAGG CAAAGCAGTT ACAGTGTTGA AGCTGCCATA CTTAAAAAGA 
CGTGGAGGCG GAGTGGATCC AGCCATCAAG GAGCGGCTCT GGTTGACACT GCACTGGCCA 
CCTGGCCATT CAACACAGAT TCTGTCCAGT TGTTTTGGGG GTGAATTGCT CCTGTGGGAT 
TTAACCAAAT CTGGGAAGCA GAAGTGGACT CTCCTTGGCA CCTCTGATGG ACAGAATCAT 
TCTCGAATTG TCTTTAACCT TTGTTCTGTG GTCACTGAGG AAGGGAAAGA GCTCTTGCTG 
TCTACTTCCA TGGACCGAGA TGTGAAATGT TGGGATCTGG GTTCCCTGTC CTGCTGCTGG 
TCCCTAACTT CCCTCGGTGG CTTTGTATAC AGCCTAGCTT TCTCCCCGGT AGCAGCTGGC 
AGCCTGGCTG TTGGGGTGGG GGACAGCATG ATCCGTGTAT GGAATACAAT GTCGGTTGTC 
AATGCATATG ATGTGAAGAC CCTGTGGCAG GGCATCAGAT CCAAGGTCAC TGCGCTGTCA 
TGGCATCCGA CCAAAGAGGG CAGTCTTGCT TTTGGCACCG AGGATGGGAA AGTTGGCATC 
TTTGAGGTCT ACATCAGCAA ACCCCCTCAG ATTTCTGCTA CATACCACAA AAAGACTGTT 
TATTCCTTAT CATGGGGACC TCTGCTGCCT CCATTCTCAT TTGGAGCAGA AGGGGACCGT 
CCTACTTATA CACTGTACAG CTGTGGGGGG GAAGGGGTTA TTTACCAGCA CAATCCTTGG 
AAACTGACAG ATGAAGCCAG TAATATTAAC AAGCTGATCC GGGACACCAA TGACATCAAA 
CACAAGTTAC CAGCACACAC AGAGTTAAGC TGGAGACCTG ATGGACAGTA TTTAGCTATT 
GGCAATGAAG ATGGAACCAT TGAGGTCTTC CACACTCAGC GTCTGCAGCT TATCTGTACC 
ATTCAGCAGC ATCACAAGCT AGTGAATGCT GTGCGCTGGC ATCATGGCCA TAGCCAAGAC 
CCTGACTTGA ACTTTCTGCT GGCATCTGGA TCTAACAATG CTACTGTCTA CGTGCACAAC 
CTGAAAGGGC CCCTGGAGAA TCCAACAGAT TCCCCAGTCA CCATCACTGA GCCTTTCCGT 
ACCTTGCCTG GACACACTGC AAAGATCACA AGCCTGTCAT GGAGCCCTCA CCACGATGCC 
AGACTGGTGT CTGCCAGTTA TGATGGTACT GCCCAGGTAT GGGATGTTCT GCAGGAAGAG 
CCACTCAGTA ACTATCGTGG GCACAAAGGA AGACTGCTTT CTGTTCAGTG GTCTCCTGTG 
GAGCCAGACC AGGTGTGGAC AGGAGCTGAT GACTTTTGCC TTCATAGCTG GTCTGTATCA 
AAGCAAGAGC ATAGCAGGCC TCCCAAAGGG AAAAAAGCCA CTGAGCTTGA AAAAAAGCGT 
ACCCTTCAAC CAAAACCAAA AGCCAAGAAG AAGAAAAAGT TAGACACCCC CAAAGAGGAG 
GCAGGAAGAG AGGTACAGAG GGGAGAAAAG GAAAGCCCTC TAACACAGAA TGGGACATCC 
GATCAAGAGG AGGGACAGGG GACTCCTGAG CCAGAGGCAG CTGACAGAGG CTTACATGAA 
GCAGATGTAG AATGTCCTTT TCAGCCAGTG GTCCCTTCTC CAGTTCCTGC TCAGGACAAG 
CACAGTCCCA TTTCATTTGC TAAGACTGTA CCAGTTAGGA AGGACCTGGC CAAAGAACGA 
GCAGATCTGC AAATCAGGAA GAAGAAGAGT CGATCGTTGC TTCCCATCAG CACCAGCAAA 
GATCACCGAT CTAAAGAAGA GCAACACCGA GACTGTGTTA AGTTGGCAAC TGTTGTATGT 
GCGAAGGATT TGGAAGAGAA ATCTCATTTT CACTCGGAGG ACACAATGCA CCTGGGACTA 
TACAGTGACC GAGCAGCCCT TTATCACCTT ATGGAGGAGG AGGGTAAGGC TCATCTGGAG 
AATGGGCACC CTGAGCTCCA GCATCAAATT ATGCTTTGGA AAGGGGATCT GAAGGGGACT 
TTGCAACTGG CTGCAGAGAG GGGTGAACTG ACTGATCAGC TGGTGGCGCT GTCTCCCGTA 
GCCGGATACA AGGTCTGGCT ATGGACCGTT GAAGCCTTTG TGAAACAGCT GTGCTTTCAT 
GAGCAGTACG TGAAAGCCGC TTCCCACCTG CTGTCCATTA ATAAAGTGTA TGAAGCCGTG 
GAACTGCTCA AGGGGAACCA CCTCTACAGA GAAGCCATTG CAGTGGCGAA GGCTCGGCTA 
CTCCCCGATG ATTCAGTCCT GCGGGAGCTG TACACTAGCT GGGCAGCAGT CCTTGAGCAG 
GACGGGCACT ATTCCATGGC TGCTAAGTGT TATTTAGGGT CCTCCTCTCC CTATGATGCT 
GCCAAGGTTC TAGCTAAGAA GGGGGATTTG CTATCCCTGA GCGCTGCTGC TGAACTTGCA 
TTAGTTGCAG GAGAAAAAGA CCTTGCTGCT TCTTTTGCCA TCCGCTGCGC CCAGGAACTG 
TTCACTGCAA AGAATTTTTT AGCAGCCCAG GAAGCACTAC GACAGCACAA CAGTCTGCTG 
GGGCAGAGAC TGCTGTTTTG TACCAGTGAG CTGCTGTACA TGCATTTGAA GGAGAAGATG 
CCCATAGATT GGAAGACACA AAGCAATCCA TGTTTCCATG AACAGACAGG CAGTAGTAAT 
GGGGAACCTT TTATGACCAA GCTTACAAGT CTATGGCAGA GTTTGTGTGA AGTAAACAGC 
CCAGAGCAAT ACAAAGATGC CTTGCAACAA CTGTCTGCCT CAGAGATTCC ACAGACCACT 
GTCAATACTG GCCCTAAACA GCTGCTGCTG CACCTTTCCC ATGAGGTCAC AATATTGTCT 
CTGAATTGTC AGCTTAAAAA CTGGAAAGAG GCTGTTCCAT CTCTCCTCCA TATCCTGTTC 
CGCTGTCAGC AGGCAGGCCA TTTCACCCTG ATGCAAGGGA TAGCCAGCCT GCTGCTTCCT 
AACGGCTGTG AAGAGCTGAG GGCTCTGTTG TCCCCAGAAA ACAAAGAGAA TTTGACTGCC 
TGTGACAGCG TGCAGGCCTT CCTTGCTTAC ATAAAGATGT ATGAGCTGTG GTGGGGACAG 
CAAGAAATGC CACCAAATTC CAAAGATCCA GCGGCTGCAG TAAATATGGA CAGTCCAAAT 
GGAGCCTCTG CTGCTGTGCA GGAGCCAGAT ATGAAACCAG GGGATTTGTG CGGCTTTCAG 
GTGTTGCTGT CAGACCTTCA CGCGCCCTTC CAGAGTGCCC AAAGAGAGAT TGCTGAGGTT 
CAGAGAGCCC TTCGGGAGAT GATACAGCAA CACCAGAGAA TCAGGCAGAA CCAGGACAGC 
TCTTTGGAAC TAGAACAAGA GCCTGAATTA AACACTTCTT CATCTCAGGA TAAATCTCTT 
GACAGCCAAG TCAGCATATC CGCTGGTCCA AGTCCTGTGT CACTGACAGA GCTGATGAAG 
AAGCTGTCAG TGGCCAACCA CCGACTAGCA GAGTTCCCTG AATATTTAAA GGCCTTACCC 
TTTCCTGATG TACTAGAGAG CTGCCTGGTG CTACTGCATC TTCCATCCTA TCAGATCTCC 
CCAGAGATCC GCTCCCAAGC CTTGGCGTTG TTGCACAGTC ATAGCCATGG ATCTGCTGAT 
CACCAAATAG CAGCCAAGAA GTTCCTCTCC ATCCCAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912844.1
CDS	96..4517
Translation	MGEPDRMLSASPNWYCSRSSDAAGGLFGFAARNSVYLLNINLQEPGFYGELVGHTDRVSGFAFSHFSGQSHLCASSSDDGTVKVWDTQTKNTVTEHHLHRSTIAALHWSPLVKDLLVSGDEKGVLVCFWCNRNDSQQFFPEPRTIFCLSCSPHKEDIVAIGYKDGMIVLVDISRRGDVLHRLRGHEEEIHCLEWSPHALECDLQQKLEETEINTANGELLPETENRRCYLASGSKDQTVRIWSASTGKAVTVLKLPYLKRRGGGVDPAIKERLWLTLHWPPGHSTQILSSCFGGELLLWDLTKSGKQKWTLLGTSDGQNHSRIVFNLCSVVTEEGKELLLSTSMDRDVKCWDLGSLSCCWSLTSLGGFVYSLAFSPVAAGSLAVGVGDSMIRVWNTMSVVNAYDVKTLWQGIRSKVTALSWHPTKEGSLAFGTEDGKVGIFEVYISKPPQISATYHKKTVYSLSWGPLLPPFSFGAEGDRPTYTLYSCGGEGVIYQHNPWKLTDEASNINKLIRDTNDIKHKLPAHTELSWRPDGQYLAIGNEDGTIEVFHTQRLQLICTIQQHHKLVNAVRWHHGHSQDPDLNFLLASGSNNATVYVHNLKGPLENPTDSPVTITEPFRTLPGHTAKITSLSWSPHHDARLVSASYDGTAQVWDVLQEEPLSNYRGHKGRLLSVQWSPVEPDQVWTGADDFCLHSWSVSKQEHSRPPKGKKATELEKKRTLQPKPKAKKKKKLDTPKEEAGREVQRGEKESPLTQNGTSDQEEGQGTPEPEAADRGLHEADVECPFQPVVPSPVPAQDKHSPISFAKTVPVRKDLAKERADLQIRKKKSRSLLPISTSKDHRSKEEQHRDCVKLATVVCAKDLEEKSHFHSEDTMHLGLYSDRAALYHLMEEEGKAHLENGHPELQHQIMLWKGDLKGTLQLAAERGELTDQLVALSPVAGYKVWLWTVEAFVKQLCFHEQYVKAASHLLSINKVYEAVELLKGNHLYREAIAVAKARLLPDDSVLRELYTSWAAVLEQDGHYSMAAKCYLGSSSPYDAAKVLAKKGDLLSLSAAAELALVAGEKDLAASFAIRCAQELFTAKNFLAAQEALRQHNSLLGQRLLFCTSELLYMHLKEKMPIDWKTQSNPCFHEQTGSSNGEPFMTKLTSLWQSLCEVNSPEQYKDALQQLSASEIPQTTVNTGPKQLLLHLSHEVTILSLNCQLKNWKEAVPSLLHILFRCQQAGHFTLMQGIASLLLPNGCEELRALLSPENKENLTACDSVQAFLAYIKMYELWWGQQEMPPNSKDPAAAVNMDSPNGASAAVQEPDMKPGDLCGFQVLLSDLHAPFQSAQREIAEVQRALREMIQQHQRIRQNQDSSLELEQEPELNTSSSQDKSLDSQVSISAGPSPVSLTELMKKLSVANHRLAEFPEYLKALPFPDVLESCLVLLHLPSYQISPEIRSQALALLHSHSHGSADHQIAAKKFLSIPE

Target ORF information:

RefSeq Version	XM_004912787.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912787.2

ORF Insert Sequence:

ATGGGGGAAC CAGATCGGAT GCTCAGTGCG TCACCCAACT GGTACTGTAG CCGCTCCAGT 
GATGCTGCCG GTGGACTGTT CGGATTCGCT GCTCGAAATT CTGTCTACCT CCTGAACATT 
AATCTGCAGG AGCCTGGATT TTATGGAGAG CTGGTTGGAC ATACAGACCG CGTCTCTGGC 
TTTGCTTTTT CCCATTTCTC AGGCCAATCT CACCTGTGTG CCAGTAGCTC TGATGATGGT 
ACTGTAAAGG TTTGGGACAC GCAGACAAAG AACACTGTAA CGGAGCACCA TCTCCATCGG 
AGTACAATTG CTGCTCTTCA CTGGTCACCT TTAGTGAAGG ATCTGCTGGT ATCCGGGGAT 
GAAAAAGGAG TATTGGTTTG TTTCTGGTGT AACCGAAATG ACAGCCAGCA GTTTTTTCCA 
GAGCCAAGGA CTATTTTCTG CCTTTCCTGC TCCCCGCACA AGGAGGACAT TGTAGCTATA 
GGGTACAAAG ATGGCATGAT AGTGCTGGTG GATATCAGCC GCAGAGGAGA TGTGTTGCAT 
CGGCTACGAG GGCATGAAGA GGAGATACAC TGTTTGGAAT GGAGCCCCCA TGCCTTGGAG 
TGTGACCTGC AACAAAAGCT GGAGGAGACA GAGATAAACA CAGCCAATGG GGAGCTTCTT 
CCTGAGACAG AAAACAGGAG ATGTTATCTG GCTTCTGGCA GCAAAGACCA GACTGTCCGT 
ATCTGGAGTG CTTCTACAGG CAAAGCAGTT ACAGTGTTGA AGCTGCCATA CTTAAAAAGA 
CGTGGAGGCG GAGTGGATCC AGCCATCAAG GAGCGGCTCT GGTTGACACT GCACTGGCCA 
CCTGGCCATT CAACACAGAT TCTGTCCAGT TGTTTTGGGG GTGAATTGCT CCTGTGGGAT 
TTAACCAAAT CTGGGAAGCA GAAGTGGACT CTCCTTGGCA CCTCTGATGG ACAGAATCAT 
TCTCGAATTG TCTTTAACCT TTGTTCTGTG GTCACTGAGG AAGGGAAAGA GCTCTTGCTG 
TCTACTTCCA TGGACCGAGA TGTGAAATGT TGGGATCTGG GTTCCCTGTC CTGCTGCTGG 
TCCCTAACTT CCCTCGGTGG CTTTGTATAC AGCCTAGCTT TCTCCCCGGT AGCAGCTGGC 
AGCCTGGCTG TTGGGGTGGG GGACAGCATG ATCCGTGTAT GGAATACAAT GTCGGTTGTC 
AATGCATATG ATGTGAAGAC CCTGTGGCAG GGCATCAGAT CCAAGGTCAC TGCGCTGTCA 
TGGCATCCGA CCAAAGAGGG CAGTCTTGCT TTTGGCACCG AGGATGGGAA AGTTGGCATC 
TTTGAGGTCT ACATCAGCAA ACCCCCTCAG ATTTCTGCTA CATACCACAA AAAGACTGTT 
TATTCCTTAT CATGGGGACC TCTGCTGCCT CCATTCTCAT TTGGAGCAGA AGGGGACCGT 
CCTACTTATA CACTGTACAG CTGTGGGGGG GAAGGGGTTA TTTACCAGCA CAATCCTTGG 
AAACTGACAG ATGAAGCCAG TAATATTAAC AAGCTGATCC GGGACACCAA TGACATCAAA 
CACAAGTTAC CAGCACACAC AGAGTTAAGC TGGAGACCTG ATGGACAGTA TTTAGCTATT 
GGCAATGAAG ATGGAACCAT TGAGGTCTTC CACACTCAGC GTCTGCAGCT TATCTGTACC 
ATTCAGCAGC ATCACAAGCT AGTGAATGCT GTGCGCTGGC ATCATGGCCA TAGCCAAGAC 
CCTGACTTGA ACTTTCTGCT GGCATCTGGA TCTAACAATG CTACTGTCTA CGTGCACAAC 
CTGAAAGGGC CCCTGGAGAA TCCAACAGAT TCCCCAGTCA CCATCACTGA GCCTTTCCGT 
ACCTTGCCTG GACACACTGC AAAGATCACA AGCCTGTCAT GGAGCCCTCA CCACGATGCC 
AGACTGGTGT CTGCCAGTTA TGATGGTACT GCCCAGGTAT GGGATGTTCT GCAGGAAGAG 
CCACTCAGTA ACTATCGTGG GCACAAAGGA AGACTGCTTT CTGTTCAGTG GTCTCCTGTG 
GAGCCAGACC AGGTGTGGAC AGGAGCTGAT GACTTTTGCC TTCATAGCTG GTCTGTATCA 
AAGCAAGAGC ATAGCAGGCC TCCCAAAGGG AAAAAAGCCA CTGAGCTTGA AAAAAAGCGT 
ACCCTTCAAC CAAAACCAAA AGCCAAGAAG AAGAAAAAGT TAGACACCCC CAAAGAGGAG 
GCAGGAAGAG AGGTACAGAG GGGAGAAAAG GAAAGCCCTC TAACACAGAA TGGGACATCC 
GATCAAGAGG AGGGACAGGG GACTCCTGAG CCAGAGGCAG CTGACAGAGG CTTACATGAA 
GCAGATGTAG AATGTCCTTT TCAGCCAGTG GTCCCTTCTC CAGTTCCTGC TCAGGACAAG 
CACAGTCCCA TTTCATTTGC TAAGACTGTA CCAGTTAGGA AGGACCTGGC CAAAGAACGA 
GCAGATCTGC AAATCAGGAA GAAGAAGAGT CGATCGTTGC TTCCCATCAG CACCAGCAAA 
GATCACCGAT CTAAAGAAGA GCAACACCGA GACTGTGTTA AGTTGGCAAC TGTTGTATGT 
GCGAAGGATT TGGAAGAGAA ATCTCATTTT CACTCGGAGG ACACAATGCA CCTGGGACTA 
TACAGTGACC GAGCAGCCCT TTATCACCTT ATGGAGGAGG AGGGTAAGGC TCATCTGGAG 
AATGGGCACC CTGAGCTCCA GCATCAAATT ATGCTTTGGA AAGGGGATCT GAAGGGGACT 
TTGCAACTGG CTGCAGAGAG GGGTGAACTG ACTGATCAGC TGGTGGCGCT GTCTCCCGTA 
GCCGGATACA AGGTCTGGCT ATGGACCGTT GAAGCCTTTG TGAAACAGCT GTGCTTTCAT 
GAGCAGTACG TGAAAGCCGC TTCCCACCTG CTGTCCATTA ATAAAGTGTA TGAAGCCGTG 
GAACTGCTCA AGGGGAACCA CCTCTACAGA GAAGCCATTG CAGTGGCGAA GGCTCGGCTA 
CTCCCCGATG ATTCAGTCCT GCGGGAGCTG TACACTAGCT GGGCAGCAGT CCTTGAGCAG 
GACGGGCACT ATTCCATGGC TGCTAAGTGT TATTTAGGGT CCTCCTCTCC CTATGATGCT 
GCCAAGGTTC TAGCTAAGAA GGGGGATTTG CTATCCCTGA GCGCTGCTGC TGAACTTGCA 
TTAGTTGCAG GAGAAAAAGA CCTTGCTGCT TCTTTTGCCA TCCGCTGCGC CCAGGAACTG 
TTCACTGCAA AGAATTTTTT AGCAGCCCAG GAAGCACTAC GACAGCACAA CAGTCTGCTG 
GGGCAGAGAC TGCTGTTTTG TACCAGTGAG CTGCTGTACA TGCATTTGAA GGAGAAGATG 
CCCATAGATT GGAAGACACA AAGCAATCCA TGTTTCCATG AACAGACAGG CAGTAGTAAT 
GGGGAACCTT TTATGACCAA GCTTACAAGT CTATGGCAGA GTTTGTGTGA AGTAAACAGC 
CCAGAGCAAT ACAAAGATGC CTTGCAACAA CTGTCTGCCT CAGAGATTCC ACAGACCACT 
GTCAATACTG GCCCTAAACA GCTGCTGCTG CACCTTTCCC ATGAGGTCAC AATATTGTCT 
CTGAATTGTC AGCTTAAAAA CTGGAAAGAG GCTGTTCCAT CTCTCCTCCA TATCCTGTTC 
CGCTGTCAGC AGGCAGGCCA TTTCACCCTG ATGCAAGGGA TAGCCAGCCT GCTGCTTCCT 
AACGGCTGTG AAGAGCTGAG GGCTCTGTTG TCCCCAGAAA ACAAAGAGAA TTTGACTGCC 
TGTGACAGCG TGCAGGCCTT CCTTGCTTAC ATAAAGATGT ATGAGCTGTG GTGGGGACAG 
CAAGAAATGC CACCAAATTC CAAAGATCCA GCGGCTGCAG TAAATATGGA CAGTCCAAAT 
GGAGCCTCTG CTGCTGTGCA GGAGCCAGAT ATGAAACCAG GGGATTTGTG CGGCTTTCAG 
GTGTTGCTGT CAGACCTTCA CGCGCCCTTC CAGAGTGCCC AAAGAGAGAT TGCTGAGGTT 
CAGAGAGCCC TTCGGGAGAT GATACAGCAA CACCAGAGAA TCAGGCAGAA CCAGGACAGC 
TCTTTGGAAC TAGAACAAGA GCCTGAATTA AACACTTCTT CATCTCAGGA TAAATCTCTT 
GACAGCCAAG TCAGCATATC CGCTGGTCCA AGTCCTGTGT CACTGACAGA GCTGATGAAG 
AAGCTGTCAG TGGCCAACCA CCGACTAGCA GAGTTCCCTG AATATTTAAA GGCCTTACCC 
TTTCCTGATG TACTAGAGAG CTGCCTGGTG CTACTGCATC TTCCATCCTA TCAGATCTCC 
CCAGAGATCC GCTCCCAAGC CTTGGCGTTG TTGCACAGTC ATAGCCATGG ATCTGCTGAT 
CACCAAATAG CAGCCAAGAA GTTCCTCTCC ATCCCAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gemin5 ( XM_031898703.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754563.1 Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X3, mRNA.

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CloneID	OXa54343
Clone ID Related Accession (Same CDS sequence)	XM_031898703.1
Accession Version	XM_031898703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gem-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAC CAGATCGGAT GCTCAGTGCG TCACCCAACT GGTACTGTAG CCGCTCCAGT 
GATGCTGCCG GTGGACTGTT CGGATTCGCT GCTCGAAATT CTGTCTACCT CCTGAACATT 
AATCTGCAGG AGCCTGGATT TTATGGAGAG CTGGTTGGAC ATACAGACCG CGTCTCTGGC 
TTTGCTTTTT CCCATTTCTC AGGCCAATCT CACCTGTGTG CCAGTAGCTC TGATGATGGT 
ACTGTAAAGG TTTGGGACAC GCAGACAAAG AACACTGTAA CGGAGCACCA TCTCCATCGG 
AGTACAATTG CTGCTCTTCA CTGGTCACCT TTAGTGAAGG ATCTGCTGGT ATCCGGGGAT 
GAAAAAGGAG TATTGGTTTG TTTCTGGTGT AACCGAAATG ACAGCCAGCA GTTTTTTCCA 
GAGCCAAGGA CTATTTTCTG CCTTTCCTGC TCCCCGCACA AGGAGGACAT TGTAGCTATA 
GGGTACAAAG ATGGCATGAT AGTGCTGGTG GATATCAGCC GCAGAGGAGA TGTGTTGCAT 
CGGCTACGAG GGCATGAAGA GGAGATACAC TGTTTGGAAT GGAGCCCCCA TGCCTTGGAG 
TGTGACCTGC AACAAAAGCT GGAGGAGACA GAGATAAACA CAGCCAATGG GGAGCTTCTT 
CCTGAGACAG AAAACAGGAG ATGTTATCTG GCTTCTGGCA GCAAAGACCA GACTGTCCGT 
ATCTGGAGTG CTTCTACAGG CAAAGCAGTT ACAGTGTTGA AGCTGCCATA CTTAAAAAGA 
CGTGGAGGCG GAGTGGATCC AGCCATCAAG GAGCGGCTCT GGTTGACACT GCACTGGCCA 
CCTGGCCATT CAACACAGAT TCTGTCCAGT TGTTTTGGGG GTGAATTGCT CCTGTGGGAT 
TTAACCAAAT CTGGGAAGCA GAAGTGGACT CTCCTTGGCA CCTCTGATGG ACAGAATCAT 
TCTCGAATTG TCTTTAACCT TTGTTCTGTG GTCACTGAGG AAGGGAAAGA GCTCTTGCTG 
TCTACTTCCA TGGACCGAGA TGTGAAATGT TGGGATCTGG GTTCCCTGTC CTGCTGCTGG 
TCCCTAACTT CCCTCGGTGG CTTTGTATAC AGCCTAGCTT TCTCCCCGGT AGCAGCTGGC 
AGCCTGGCTG TTGGGGTGGG GGACAGCATG ATCCGTGTAT GGAATACAAT GTCGGTTGTC 
AATGCATATG ATGTGAAGAC CCTGTGGCAG GGCATCAGAT CCAAGGTCAC TGCGCTGTCA 
TGGCATCCGA CCAAAGAGGG CAGTCTTGCT TTTGGCACCG AGGATGGGAA AGTTGGCATC 
TTTGAGGTCT ACATCAGCAA ACCCCCTCAG ATTTCTGCTA CATACCACAA AAAGACTGTT 
TATTCCTTAT CATGGGGACC TCTGCTGCCT CCATTCTCAT TTGGAGCAGA AGGGGACCGT 
CCTACTTATA CACTGTACAG CTGTGGGGGG GAAGGGGTTA TTTACCAGCA CAATCCTTGG 
AAACTGACAG ATGAAGCCAG TAATATTAAC AAGCTGATCC GGGACACCAA TGACATCAAA 
CACAAGTTAC CAGCACACAC AGAGTTAAGC TGGAGACCTG ATGGACAGTA TTTAGCTATT 
GGCAATGAAG ATGGAACCAT TGAGGTCTTC CACACTCAGC GTCTGCAGCT TATCTGTACC 
ATTCAGCAGC ATCACAAGCT AGTGAATGCT GTGCGCTGGC ATCATGGCCA TAGCCAAGAC 
CCTGACTTGA ACTTTCTGCT GGCATCTGGA TCTAACAATG CTACTGTCTA CGTGCACAAC 
CTGAAAGGGC CCCTGGAGAA TCCAACAGAT TCCCCAGTCA CCATCACTGA GCCTTTCCGT 
ACCTTGCCTG GACACACTGC AAAGATCACA AGCCTGTCAT GGAGCCCTCA CCACGATGCC 
AGACTGGTGT CTGCCAGTTA TGATGGTACT GCCCAGGTAT GGGATGTTCT GCAGGAAGAG 
CCACTCAGTA ACTATCGTGG GCACAAAGGA AGACTGCTTT CTGTTCAGTG GTCTCCTGTG 
GAGCCAGACC AGGTGTGGAC AGGAGCTGAT GACTTTTGCC TTCATAGCTG GTCTGTATCA 
AAGCAAGAGC ATAGCAGGCC TCCCAAAGGG AAAAAAGCCA CTGAGCTTGA AAAAAAGCGT 
ACCCTTCAAC CAAAACCAAA AGCCAAGAAG AAGAAAAAGT TAGACACCCC CAAAGAGGAG 
GCAGGAAGAG AGGTACAGAG GGGAGAAAAG GAAAGCCCTC TAACACAGAA TGGGACATCC 
GATCAAGAGG AGGGACAGGG GACTCCTGAG CCAGAGGCAG CTGACAGAGG CTTACATGAA 
GATGTAGAAT GTCCTTTTCA GCCAGTGGTC CCTTCTCCAG TTCCTGCTCA GGACAAGCAC 
AGTCCCATTT CATTTGCTAA GACTGTACCA GTTAGGAAGG ACCTGGCCAA AGAACGAGCA 
GATCTGCAAA TCAGGAAGAA GAAGAGTCGA TCGTTGCTTC CCATCAGCAC CAGCAAAGAT 
CACCGATCTA AAGAAGAGCA ACACCGAGAC TGTGTTAAGT TGGCAACTGT TGTATGTGCG 
AAGGATTTGG AAGAGAAATC TCATTTTCAC TCGGAGGACA CAATGCACCT GGGACTATAC 
AGTGACCGAG CAGCCCTTTA TCACCTTATG GAGGAGGAGG GTAAGGCTCA TCTGGAGAAT 
GGGCACCCTG AGCTCCAGCA TCAAATTATG CTTTGGAAAG GGGATCTGAA GGGGACTTTG 
CAACTGGCTG CAGAGAGGGG TGAACTGACT GATCAGCTGG TGGCGCTGTC TCCCGTAGCC 
GGATACAAGG TCTGGCTATG GACCGTTGAA GCCTTTGTGA AACAGCTGTG CTTTCATGAG 
CAGTACGTGA AAGCCGCTTC CCACCTGCTG TCCATTAATA AAGTGTATGA AGCCGTGGAA 
CTGCTCAAGG GGAACCACCT CTACAGAGAA GCCATTGCAG TGGCGAAGGC TCGGCTACTC 
CCCGATGATT CAGTCCTGCG GGAGCTGTAC ACTAGCTGGG CAGCAGTCCT TGAGCAGGAC 
GGGCACTATT CCATGGCTGC TAAGTGTTAT TTAGGGTCCT CCTCTCCCTA TGATGCTGCC 
AAGGTTCTAG CTAAGAAGGG GGATTTGCTA TCCCTGAGCG CTGCTGCTGA ACTTGCATTA 
GTTGCAGGAG AAAAAGACCT TGCTGCTTCT TTTGCCATCC GCTGCGCCCA GGAACTGTTC 
ACTGCAAAGA ATTTTTTAGC AGCCCAGGAA GCACTACGAC AGCACAACAG TCTGCTGGGG 
CAGAGACTGC TGTTTTGTAC CAGTGAGCTG CTGTACATGC ATTTGAAGGA GAAGATGCCC 
ATAGATTGGA AGACACAAAG CAATCCATGT TTCCATGAAC AGACAGGCAG TAGTAATGGG 
GAACCTTTTA TGACCAAGCT TACAAGTCTA TGGCAGAGTT TGTGTGAAGT AAACAGCCCA 
GAGCAATACA AAGATGCCTT GCAACAACTG TCTGCCTCAG AGATTCCACA GACCACTGTC 
AATACTGGCC CTAAACAGCT GCTGCTGCAC CTTTCCCATG AGGTCACAAT ATTGTCTCTG 
AATTGTCAGC TTAAAAACTG GAAAGAGGCT GTTCCATCTC TCCTCCATAT CCTGTTCCGC 
TGTCAGCAGG CAGGCCATTT CACCCTGATG CAAGGGATAG CCAGCCTGCT GCTTCCTAAC 
GGCTGTGAAG AGCTGAGGGC TCTGTTGTCC CCAGAAAACA AAGAGAATTT GACTGCCTGT 
GACAGCGTGC AGGCCTTCCT TGCTTACATA AAGATGTATG AGCTGTGGTG GGGACAGCAA 
GAAATGCCAC CAAATTCCAA AGATCCAGCG GCTGCAGTAA ATATGGACAG TCCAAATGGA 
GCCTCTGCTG CTGTGCAGGA GCCAGATATG AAACCAGGGG ATTTGTGCGG CTTTCAGGTG 
TTGCTGTCAG ACCTTCACGC GCCCTTCCAG AGTGCCCAAA GAGAGATTGC TGAGGTTCAG 
AGAGCCCTTC GGGAGATGAT ACAGCAACAC CAGAGAATCA GGCAGAACCA GGACAGCTCT 
TTGGAACTAG AACAAGAGCC TGAATTAAAC ACTTCTTCAT CTCAGGATAA ATCTCTTGAC 
AGCCAAGTCA GCATATCCGC TGGTCCAAGT CCTGTGTCAC TGACAGAGCT GATGAAGAAG 
CTGTCAGTGG CCAACCACCG ACTAGCAGAG TTCCCTGAAT ATTTAAAGGC CTTACCCTTT 
CCTGATGTAC TAGAGAGCTG CCTGGTGCTA CTGCATCTTC CATCCTATCA GATCTCCCCA 
GAGATCCGCT CCCAAGCCTT GGCGTTGTTG CACAGTCATA GCCATGGATC TGCTGATCAC 
CAAATAGCAG CCAAGAAGTT CCTCTCCATC CCAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754563.1
CDS	96..4514
Translation	MGEPDRMLSASPNWYCSRSSDAAGGLFGFAARNSVYLLNINLQEPGFYGELVGHTDRVSGFAFSHFSGQSHLCASSSDDGTVKVWDTQTKNTVTEHHLHRSTIAALHWSPLVKDLLVSGDEKGVLVCFWCNRNDSQQFFPEPRTIFCLSCSPHKEDIVAIGYKDGMIVLVDISRRGDVLHRLRGHEEEIHCLEWSPHALECDLQQKLEETEINTANGELLPETENRRCYLASGSKDQTVRIWSASTGKAVTVLKLPYLKRRGGGVDPAIKERLWLTLHWPPGHSTQILSSCFGGELLLWDLTKSGKQKWTLLGTSDGQNHSRIVFNLCSVVTEEGKELLLSTSMDRDVKCWDLGSLSCCWSLTSLGGFVYSLAFSPVAAGSLAVGVGDSMIRVWNTMSVVNAYDVKTLWQGIRSKVTALSWHPTKEGSLAFGTEDGKVGIFEVYISKPPQISATYHKKTVYSLSWGPLLPPFSFGAEGDRPTYTLYSCGGEGVIYQHNPWKLTDEASNINKLIRDTNDIKHKLPAHTELSWRPDGQYLAIGNEDGTIEVFHTQRLQLICTIQQHHKLVNAVRWHHGHSQDPDLNFLLASGSNNATVYVHNLKGPLENPTDSPVTITEPFRTLPGHTAKITSLSWSPHHDARLVSASYDGTAQVWDVLQEEPLSNYRGHKGRLLSVQWSPVEPDQVWTGADDFCLHSWSVSKQEHSRPPKGKKATELEKKRTLQPKPKAKKKKKLDTPKEEAGREVQRGEKESPLTQNGTSDQEEGQGTPEPEAADRGLHEDVECPFQPVVPSPVPAQDKHSPISFAKTVPVRKDLAKERADLQIRKKKSRSLLPISTSKDHRSKEEQHRDCVKLATVVCAKDLEEKSHFHSEDTMHLGLYSDRAALYHLMEEEGKAHLENGHPELQHQIMLWKGDLKGTLQLAAERGELTDQLVALSPVAGYKVWLWTVEAFVKQLCFHEQYVKAASHLLSINKVYEAVELLKGNHLYREAIAVAKARLLPDDSVLRELYTSWAAVLEQDGHYSMAAKCYLGSSSPYDAAKVLAKKGDLLSLSAAAELALVAGEKDLAASFAIRCAQELFTAKNFLAAQEALRQHNSLLGQRLLFCTSELLYMHLKEKMPIDWKTQSNPCFHEQTGSSNGEPFMTKLTSLWQSLCEVNSPEQYKDALQQLSASEIPQTTVNTGPKQLLLHLSHEVTILSLNCQLKNWKEAVPSLLHILFRCQQAGHFTLMQGIASLLLPNGCEELRALLSPENKENLTACDSVQAFLAYIKMYELWWGQQEMPPNSKDPAAAVNMDSPNGASAAVQEPDMKPGDLCGFQVLLSDLHAPFQSAQREIAEVQRALREMIQQHQRIRQNQDSSLELEQEPELNTSSSQDKSLDSQVSISAGPSPVSLTELMKKLSVANHRLAEFPEYLKALPFPDVLESCLVLLHLPSYQISPEIRSQALALLHSHSHGSADHQIAAKKFLSIPE

Target ORF information:

RefSeq Version	XM_031898703.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898703.1

ORF Insert Sequence:

ATGGGGGAAC CAGATCGGAT GCTCAGTGCG TCACCCAACT GGTACTGTAG CCGCTCCAGT 
GATGCTGCCG GTGGACTGTT CGGATTCGCT GCTCGAAATT CTGTCTACCT CCTGAACATT 
AATCTGCAGG AGCCTGGATT TTATGGAGAG CTGGTTGGAC ATACAGACCG CGTCTCTGGC 
TTTGCTTTTT CCCATTTCTC AGGCCAATCT CACCTGTGTG CCAGTAGCTC TGATGATGGT 
ACTGTAAAGG TTTGGGACAC GCAGACAAAG AACACTGTAA CGGAGCACCA TCTCCATCGG 
AGTACAATTG CTGCTCTTCA CTGGTCACCT TTAGTGAAGG ATCTGCTGGT ATCCGGGGAT 
GAAAAAGGAG TATTGGTTTG TTTCTGGTGT AACCGAAATG ACAGCCAGCA GTTTTTTCCA 
GAGCCAAGGA CTATTTTCTG CCTTTCCTGC TCCCCGCACA AGGAGGACAT TGTAGCTATA 
GGGTACAAAG ATGGCATGAT AGTGCTGGTG GATATCAGCC GCAGAGGAGA TGTGTTGCAT 
CGGCTACGAG GGCATGAAGA GGAGATACAC TGTTTGGAAT GGAGCCCCCA TGCCTTGGAG 
TGTGACCTGC AACAAAAGCT GGAGGAGACA GAGATAAACA CAGCCAATGG GGAGCTTCTT 
CCTGAGACAG AAAACAGGAG ATGTTATCTG GCTTCTGGCA GCAAAGACCA GACTGTCCGT 
ATCTGGAGTG CTTCTACAGG CAAAGCAGTT ACAGTGTTGA AGCTGCCATA CTTAAAAAGA 
CGTGGAGGCG GAGTGGATCC AGCCATCAAG GAGCGGCTCT GGTTGACACT GCACTGGCCA 
CCTGGCCATT CAACACAGAT TCTGTCCAGT TGTTTTGGGG GTGAATTGCT CCTGTGGGAT 
TTAACCAAAT CTGGGAAGCA GAAGTGGACT CTCCTTGGCA CCTCTGATGG ACAGAATCAT 
TCTCGAATTG TCTTTAACCT TTGTTCTGTG GTCACTGAGG AAGGGAAAGA GCTCTTGCTG 
TCTACTTCCA TGGACCGAGA TGTGAAATGT TGGGATCTGG GTTCCCTGTC CTGCTGCTGG 
TCCCTAACTT CCCTCGGTGG CTTTGTATAC AGCCTAGCTT TCTCCCCGGT AGCAGCTGGC 
AGCCTGGCTG TTGGGGTGGG GGACAGCATG ATCCGTGTAT GGAATACAAT GTCGGTTGTC 
AATGCATATG ATGTGAAGAC CCTGTGGCAG GGCATCAGAT CCAAGGTCAC TGCGCTGTCA 
TGGCATCCGA CCAAAGAGGG CAGTCTTGCT TTTGGCACCG AGGATGGGAA AGTTGGCATC 
TTTGAGGTCT ACATCAGCAA ACCCCCTCAG ATTTCTGCTA CATACCACAA AAAGACTGTT 
TATTCCTTAT CATGGGGACC TCTGCTGCCT CCATTCTCAT TTGGAGCAGA AGGGGACCGT 
CCTACTTATA CACTGTACAG CTGTGGGGGG GAAGGGGTTA TTTACCAGCA CAATCCTTGG 
AAACTGACAG ATGAAGCCAG TAATATTAAC AAGCTGATCC GGGACACCAA TGACATCAAA 
CACAAGTTAC CAGCACACAC AGAGTTAAGC TGGAGACCTG ATGGACAGTA TTTAGCTATT 
GGCAATGAAG ATGGAACCAT TGAGGTCTTC CACACTCAGC GTCTGCAGCT TATCTGTACC 
ATTCAGCAGC ATCACAAGCT AGTGAATGCT GTGCGCTGGC ATCATGGCCA TAGCCAAGAC 
CCTGACTTGA ACTTTCTGCT GGCATCTGGA TCTAACAATG CTACTGTCTA CGTGCACAAC 
CTGAAAGGGC CCCTGGAGAA TCCAACAGAT TCCCCAGTCA CCATCACTGA GCCTTTCCGT 
ACCTTGCCTG GACACACTGC AAAGATCACA AGCCTGTCAT GGAGCCCTCA CCACGATGCC 
AGACTGGTGT CTGCCAGTTA TGATGGTACT GCCCAGGTAT GGGATGTTCT GCAGGAAGAG 
CCACTCAGTA ACTATCGTGG GCACAAAGGA AGACTGCTTT CTGTTCAGTG GTCTCCTGTG 
GAGCCAGACC AGGTGTGGAC AGGAGCTGAT GACTTTTGCC TTCATAGCTG GTCTGTATCA 
AAGCAAGAGC ATAGCAGGCC TCCCAAAGGG AAAAAAGCCA CTGAGCTTGA AAAAAAGCGT 
ACCCTTCAAC CAAAACCAAA AGCCAAGAAG AAGAAAAAGT TAGACACCCC CAAAGAGGAG 
GCAGGAAGAG AGGTACAGAG GGGAGAAAAG GAAAGCCCTC TAACACAGAA TGGGACATCC 
GATCAAGAGG AGGGACAGGG GACTCCTGAG CCAGAGGCAG CTGACAGAGG CTTACATGAA 
GATGTAGAAT GTCCTTTTCA GCCAGTGGTC CCTTCTCCAG TTCCTGCTCA GGACAAGCAC 
AGTCCCATTT CATTTGCTAA GACTGTACCA GTTAGGAAGG ACCTGGCCAA AGAACGAGCA 
GATCTGCAAA TCAGGAAGAA GAAGAGTCGA TCGTTGCTTC CCATCAGCAC CAGCAAAGAT 
CACCGATCTA AAGAAGAGCA ACACCGAGAC TGTGTTAAGT TGGCAACTGT TGTATGTGCG 
AAGGATTTGG AAGAGAAATC TCATTTTCAC TCGGAGGACA CAATGCACCT GGGACTATAC 
AGTGACCGAG CAGCCCTTTA TCACCTTATG GAGGAGGAGG GTAAGGCTCA TCTGGAGAAT 
GGGCACCCTG AGCTCCAGCA TCAAATTATG CTTTGGAAAG GGGATCTGAA GGGGACTTTG 
CAACTGGCTG CAGAGAGGGG TGAACTGACT GATCAGCTGG TGGCGCTGTC TCCCGTAGCC 
GGATACAAGG TCTGGCTATG GACCGTTGAA GCCTTTGTGA AACAGCTGTG CTTTCATGAG 
CAGTACGTGA AAGCCGCTTC CCACCTGCTG TCCATTAATA AAGTGTATGA AGCCGTGGAA 
CTGCTCAAGG GGAACCACCT CTACAGAGAA GCCATTGCAG TGGCGAAGGC TCGGCTACTC 
CCCGATGATT CAGTCCTGCG GGAGCTGTAC ACTAGCTGGG CAGCAGTCCT TGAGCAGGAC 
GGGCACTATT CCATGGCTGC TAAGTGTTAT TTAGGGTCCT CCTCTCCCTA TGATGCTGCC 
AAGGTTCTAG CTAAGAAGGG GGATTTGCTA TCCCTGAGCG CTGCTGCTGA ACTTGCATTA 
GTTGCAGGAG AAAAAGACCT TGCTGCTTCT TTTGCCATCC GCTGCGCCCA GGAACTGTTC 
ACTGCAAAGA ATTTTTTAGC AGCCCAGGAA GCACTACGAC AGCACAACAG TCTGCTGGGG 
CAGAGACTGC TGTTTTGTAC CAGTGAGCTG CTGTACATGC ATTTGAAGGA GAAGATGCCC 
ATAGATTGGA AGACACAAAG CAATCCATGT TTCCATGAAC AGACAGGCAG TAGTAATGGG 
GAACCTTTTA TGACCAAGCT TACAAGTCTA TGGCAGAGTT TGTGTGAAGT AAACAGCCCA 
GAGCAATACA AAGATGCCTT GCAACAACTG TCTGCCTCAG AGATTCCACA GACCACTGTC 
AATACTGGCC CTAAACAGCT GCTGCTGCAC CTTTCCCATG AGGTCACAAT ATTGTCTCTG 
AATTGTCAGC TTAAAAACTG GAAAGAGGCT GTTCCATCTC TCCTCCATAT CCTGTTCCGC 
TGTCAGCAGG CAGGCCATTT CACCCTGATG CAAGGGATAG CCAGCCTGCT GCTTCCTAAC 
GGCTGTGAAG AGCTGAGGGC TCTGTTGTCC CCAGAAAACA AAGAGAATTT GACTGCCTGT 
GACAGCGTGC AGGCCTTCCT TGCTTACATA AAGATGTATG AGCTGTGGTG GGGACAGCAA 
GAAATGCCAC CAAATTCCAA AGATCCAGCG GCTGCAGTAA ATATGGACAG TCCAAATGGA 
GCCTCTGCTG CTGTGCAGGA GCCAGATATG AAACCAGGGG ATTTGTGCGG CTTTCAGGTG 
TTGCTGTCAG ACCTTCACGC GCCCTTCCAG AGTGCCCAAA GAGAGATTGC TGAGGTTCAG 
AGAGCCCTTC GGGAGATGAT ACAGCAACAC CAGAGAATCA GGCAGAACCA GGACAGCTCT 
TTGGAACTAG AACAAGAGCC TGAATTAAAC ACTTCTTCAT CTCAGGATAA ATCTCTTGAC 
AGCCAAGTCA GCATATCCGC TGGTCCAAGT CCTGTGTCAC TGACAGAGCT GATGAAGAAG 
CTGTCAGTGG CCAACCACCG ACTAGCAGAG TTCCCTGAAT ATTTAAAGGC CTTACCCTTT 
CCTGATGTAC TAGAGAGCTG CCTGGTGCTA CTGCATCTTC CATCCTATCA GATCTCCCCA 
GAGATCCGCT CCCAAGCCTT GGCGTTGTTG CACAGTCATA GCCATGGATC TGCTGATCAC 
CAAATAGCAG CCAAGAAGTT CCTCTCCATC CCAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gemin5 ( XM_031898704.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754564.1 Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X4, mRNA.

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CloneID	OXa54344
Clone ID Related Accession (Same CDS sequence)	XM_031898704.1
Accession Version	XM_031898704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gem-associated protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAC CAGATCGGAT GCTCAGTGCG TCACCCAACT GGTACTGTAG CCGCTCCAGT 
GATGCTGCCG GTGGACTGTT CGGATTCGCT GCTCGAAATT CTGTCTACCT CCTGAACATT 
AATCTGCAGG AGCCTGGATT TTATGGAGAG CTGGTTGGAC ATACAGACCG CGTCTCTGGC 
TTTGCTTTTT CCCATTTCTC AGGCCAATCT CACCTGTGTG CCAGTAGCTC TGATGATGGT 
ACTGTAAAGG TTTGGGACAC GCAGACAAAG AACACTGTAA CGGAGCACCA TCTCCATCGG 
AGTACAATTG CTGCTCTTCA CTGGTCACCT TTAGTGAAGG ATCTGCTGGT ATCCGGGGAT 
GAAAAAGGAG TATTGGTTTG TTTCTGGTGT AACCGAAATG ACAGCCAGCA GTTTTTTCCA 
GAGCCAAGGA CTATTTTCTG CCTTTCCTGC TCCCCGCACA AGGAGGACAT TGTAGCTATA 
GGGTACAAAG ATGGCATGAT AGTGCTGGTG GATATCAGCC GCAGAGGAGA TGTGTTGCAT 
CGGCTACGAG GGCATGAAGA GGAGATACAC TGTTTGGAAT GGAGCCCCCA TGCCTTGGAG 
TGTGACCTGC AACAAAAGCT GGAGGAGACA GAGATAAACA CAGCCAATGG GGAGCTTCTT 
CCTGAGACAG AAAACAGGAG ATGTTATCTG GCTTCTGGCA GCAAAGACCA GACTGTCCGT 
ATCTGGAGTG CTTCTACAGG CAAAGCAGTT ACAGTGTTGA AGCTGCCATA CTTAAAAAGA 
CGTGGAGGCG GAGTGGATCC AGCCATCAAG GAGCGGCTCT GGTTGACACT GCACTGGCCA 
CCTGGCCATT CAACACAGAT TCTGTCCAGT TGTTTTGGGG GTGAATTGCT CCTGTGGGAT 
TTAACCAAAT CTGGGAAGCA GAAGTGGACT CTCCTTGGCA CCTCTGATGG ACAGAATCAT 
TCTCGAATTG TCTTTAACCT TTGTTCTGTG GTCACTGAGG AAGGGAAAGA GCTCTTGCTG 
TCTACTTCCA TGGACCGAGA TGTGAAATGT TGGGATCTGG GTTCCCTGTC CTGCTGCTGG 
TCCCTAACTT CCCTCGGTGG CTTTGTATAC AGCCTAGCTT TCTCCCCGGT AGCAGCTGGC 
AGCCTGGCTG TTGGGGTGGG GGACAGCATG ATCCGTGTAT GGAATACAAT GTCGGTTGTC 
AATGCATATG ATGTGAAGAC CCTGTGGCAG GGCATCAGAT CCAAGGTCAC TGCGCTGTCA 
TGGCATCCGA CCAAAGAGGG CAGTCTTGCT TTTGGCACCG AGGATGGGAA AGTTGGCATC 
TTTGAGGTCT ACATCAGCAA ACCCCCTCAG ATTTCTGCTA CATACCACAA AAAGACTGTT 
TATTCCTTAT CATGGGGACC TCTGCTGCCT CCATTCTCAT TTGGAGCAGA AGGGGACCGT 
CCTACTTATA CACTGTACAG CTGTGGGGGG GAAGGGGTTA TTTACCAGCA CAATCCTTGG 
AAACTGACAG ATGAAGCCAG TAATATTAAC AAGCTGATCC GGGACACCAA TGACATCAAA 
CACAAGTTAC CAGCACACAC AGAGTTAAGC TGGAGACCTG ATGGACAGTA TTTAGCTATT 
GGCAATGAAG ATGGAACCAT TGAGGTCTTC CACACTCAGC GTCTGCAGCT TATCTGTACC 
ATTCAGCAGC ATCACAAGCT AGTGAATGCT GTGCGCTGGC ATCATGGCCA TAGCCAAGAC 
CCTGACTTGA ACTTTCTGCT GGCATCTGGA TCTAACAATG CTACTGTCTA CGTGCACAAC 
CTGAAAGGGC CCCTGGAGAA TCCAACAGAT TCCCCAGTCA CCATCACTGA GCCTTTCCGT 
ACCTTGCCTG GACACACTGC AAAGATCACA AGCCTGTCAT GGAGCCCTCA CCACGATGCC 
AGACTGGTGT CTGCCAGTTA TGATGGTACT GCCCAGGTAT GGGATGTTCT GCAGGAAGAG 
CCACTCAGTA ACTATCGTGG GCACAAAGGA AGACTGCTTT CTGTTCAGTG GTCTCCTGTG 
GAGCCAGACC AGGTGTGGAC AGGAGCTGAT GACTTTTGCC TTCATAGCTG GTCTGTATCA 
AAGCAAGAGC ATAGCAGGCC TCCCAAAGGG AAAAAAGCCA CTGAGCTTGA AAAAAAGCGT 
ACCCTTCAAC CAAAACCAAA AGCCAAGAAG AAGAAAAAGT TAGACACCCC CAAAGAGGAG 
GCAGGAAGAG AGGTACAGAG GGGAGAAAAG GAAAGCCCTC TAACACAGAA TGGGACATCC 
GATCAAGAGG AGGGACAGGG GACTCCTGAG CCAGAGGCAG CTGACAGAGG CTTACATGAA 
GATCTGCAAA TCAGGAAGAA GAAGAGTCGA TCGTTGCTTC CCATCAGCAC CAGCAAAGAT 
CACCGATCTA AAGAAGAGCA ACACCGAGAC TGTGTTAAGT TGGCAACTGT TGTATGTGCG 
AAGGATTTGG AAGAGAAATC TCATTTTCAC TCGGAGGACA CAATGCACCT GGGACTATAC 
AGTGACCGAG CAGCCCTTTA TCACCTTATG GAGGAGGAGG GTAAGGCTCA TCTGGAGAAT 
GGGCACCCTG AGCTCCAGCA TCAAATTATG CTTTGGAAAG GGGATCTGAA GGGGACTTTG 
CAACTGGCTG CAGAGAGGGG TGAACTGACT GATCAGCTGG TGGCGCTGTC TCCCGTAGCC 
GGATACAAGG TCTGGCTATG GACCGTTGAA GCCTTTGTGA AACAGCTGTG CTTTCATGAG 
CAGTACGTGA AAGCCGCTTC CCACCTGCTG TCCATTAATA AAGTGTATGA AGCCGTGGAA 
CTGCTCAAGG GGAACCACCT CTACAGAGAA GCCATTGCAG TGGCGAAGGC TCGGCTACTC 
CCCGATGATT CAGTCCTGCG GGAGCTGTAC ACTAGCTGGG CAGCAGTCCT TGAGCAGGAC 
GGGCACTATT CCATGGCTGC TAAGTGTTAT TTAGGGTCCT CCTCTCCCTA TGATGCTGCC 
AAGGTTCTAG CTAAGAAGGG GGATTTGCTA TCCCTGAGCG CTGCTGCTGA ACTTGCATTA 
GTTGCAGGAG AAAAAGACCT TGCTGCTTCT TTTGCCATCC GCTGCGCCCA GGAACTGTTC 
ACTGCAAAGA ATTTTTTAGC AGCCCAGGAA GCACTACGAC AGCACAACAG TCTGCTGGGG 
CAGAGACTGC TGTTTTGTAC CAGTGAGCTG CTGTACATGC ATTTGAAGGA GAAGATGCCC 
ATAGATTGGA AGACACAAAG CAATCCATGT TTCCATGAAC AGACAGGCAG TAGTAATGGG 
GAACCTTTTA TGACCAAGCT TACAAGTCTA TGGCAGAGTT TGTGTGAAGT AAACAGCCCA 
GAGCAATACA AAGATGCCTT GCAACAACTG TCTGCCTCAG AGATTCCACA GACCACTGTC 
AATACTGGCC CTAAACAGCT GCTGCTGCAC CTTTCCCATG AGGTCACAAT ATTGTCTCTG 
AATTGTCAGC TTAAAAACTG GAAAGAGGCT GTTCCATCTC TCCTCCATAT CCTGTTCCGC 
TGTCAGCAGG CAGGCCATTT CACCCTGATG CAAGGGATAG CCAGCCTGCT GCTTCCTAAC 
GGCTGTGAAG AGCTGAGGGC TCTGTTGTCC CCAGAAAACA AAGAGAATTT GACTGCCTGT 
GACAGCGTGC AGGCCTTCCT TGCTTACATA AAGATGTATG AGCTGTGGTG GGGACAGCAA 
GAAATGCCAC CAAATTCCAA AGATCCAGCG GCTGCAGTAA ATATGGACAG TCCAAATGGA 
GCCTCTGCTG CTGTGCAGGA GCCAGATATG AAACCAGGGG ATTTGTGCGG CTTTCAGGTG 
TTGCTGTCAG ACCTTCACGC GCCCTTCCAG AGTGCCCAAA GAGAGATTGC TGAGGTTCAG 
AGAGCCCTTC GGGAGATGAT ACAGCAACAC CAGAGAATCA GGCAGAACCA GGACAGCTCT 
TTGGAACTAG AACAAGAGCC TGAATTAAAC ACTTCTTCAT CTCAGGATAA ATCTCTTGAC 
AGCCAAGTCA GCATATCCGC TGGTCCAAGT CCTGTGTCAC TGACAGAGCT GATGAAGAAG 
CTGTCAGTGG CCAACCACCG ACTAGCAGAG TTCCCTGAAT ATTTAAAGGC CTTACCCTTT 
CCTGATGTAC TAGAGAGCTG CCTGGTGCTA CTGCATCTTC CATCCTATCA GATCTCCCCA 
GAGATCCGCT CCCAAGCCTT GGCGTTGTTG CACAGTCATA GCCATGGATC TGCTGATCAC 
CAAATAGCAG CCAAGAAGTT CCTCTCCATC CCAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754564.1
CDS	96..4394
Translation	MGEPDRMLSASPNWYCSRSSDAAGGLFGFAARNSVYLLNINLQEPGFYGELVGHTDRVSGFAFSHFSGQSHLCASSSDDGTVKVWDTQTKNTVTEHHLHRSTIAALHWSPLVKDLLVSGDEKGVLVCFWCNRNDSQQFFPEPRTIFCLSCSPHKEDIVAIGYKDGMIVLVDISRRGDVLHRLRGHEEEIHCLEWSPHALECDLQQKLEETEINTANGELLPETENRRCYLASGSKDQTVRIWSASTGKAVTVLKLPYLKRRGGGVDPAIKERLWLTLHWPPGHSTQILSSCFGGELLLWDLTKSGKQKWTLLGTSDGQNHSRIVFNLCSVVTEEGKELLLSTSMDRDVKCWDLGSLSCCWSLTSLGGFVYSLAFSPVAAGSLAVGVGDSMIRVWNTMSVVNAYDVKTLWQGIRSKVTALSWHPTKEGSLAFGTEDGKVGIFEVYISKPPQISATYHKKTVYSLSWGPLLPPFSFGAEGDRPTYTLYSCGGEGVIYQHNPWKLTDEASNINKLIRDTNDIKHKLPAHTELSWRPDGQYLAIGNEDGTIEVFHTQRLQLICTIQQHHKLVNAVRWHHGHSQDPDLNFLLASGSNNATVYVHNLKGPLENPTDSPVTITEPFRTLPGHTAKITSLSWSPHHDARLVSASYDGTAQVWDVLQEEPLSNYRGHKGRLLSVQWSPVEPDQVWTGADDFCLHSWSVSKQEHSRPPKGKKATELEKKRTLQPKPKAKKKKKLDTPKEEAGREVQRGEKESPLTQNGTSDQEEGQGTPEPEAADRGLHEDLQIRKKKSRSLLPISTSKDHRSKEEQHRDCVKLATVVCAKDLEEKSHFHSEDTMHLGLYSDRAALYHLMEEEGKAHLENGHPELQHQIMLWKGDLKGTLQLAAERGELTDQLVALSPVAGYKVWLWTVEAFVKQLCFHEQYVKAASHLLSINKVYEAVELLKGNHLYREAIAVAKARLLPDDSVLRELYTSWAAVLEQDGHYSMAAKCYLGSSSPYDAAKVLAKKGDLLSLSAAAELALVAGEKDLAASFAIRCAQELFTAKNFLAAQEALRQHNSLLGQRLLFCTSELLYMHLKEKMPIDWKTQSNPCFHEQTGSSNGEPFMTKLTSLWQSLCEVNSPEQYKDALQQLSASEIPQTTVNTGPKQLLLHLSHEVTILSLNCQLKNWKEAVPSLLHILFRCQQAGHFTLMQGIASLLLPNGCEELRALLSPENKENLTACDSVQAFLAYIKMYELWWGQQEMPPNSKDPAAAVNMDSPNGASAAVQEPDMKPGDLCGFQVLLSDLHAPFQSAQREIAEVQRALREMIQQHQRIRQNQDSSLELEQEPELNTSSSQDKSLDSQVSISAGPSPVSLTELMKKLSVANHRLAEFPEYLKALPFPDVLESCLVLLHLPSYQISPEIRSQALALLHSHSHGSADHQIAAKKFLSIPE

Target ORF information:

RefSeq Version	XM_031898704.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898704.1

ORF Insert Sequence:

ATGGGGGAAC CAGATCGGAT GCTCAGTGCG TCACCCAACT GGTACTGTAG CCGCTCCAGT 
GATGCTGCCG GTGGACTGTT CGGATTCGCT GCTCGAAATT CTGTCTACCT CCTGAACATT 
AATCTGCAGG AGCCTGGATT TTATGGAGAG CTGGTTGGAC ATACAGACCG CGTCTCTGGC 
TTTGCTTTTT CCCATTTCTC AGGCCAATCT CACCTGTGTG CCAGTAGCTC TGATGATGGT 
ACTGTAAAGG TTTGGGACAC GCAGACAAAG AACACTGTAA CGGAGCACCA TCTCCATCGG 
AGTACAATTG CTGCTCTTCA CTGGTCACCT TTAGTGAAGG ATCTGCTGGT ATCCGGGGAT 
GAAAAAGGAG TATTGGTTTG TTTCTGGTGT AACCGAAATG ACAGCCAGCA GTTTTTTCCA 
GAGCCAAGGA CTATTTTCTG CCTTTCCTGC TCCCCGCACA AGGAGGACAT TGTAGCTATA 
GGGTACAAAG ATGGCATGAT AGTGCTGGTG GATATCAGCC GCAGAGGAGA TGTGTTGCAT 
CGGCTACGAG GGCATGAAGA GGAGATACAC TGTTTGGAAT GGAGCCCCCA TGCCTTGGAG 
TGTGACCTGC AACAAAAGCT GGAGGAGACA GAGATAAACA CAGCCAATGG GGAGCTTCTT 
CCTGAGACAG AAAACAGGAG ATGTTATCTG GCTTCTGGCA GCAAAGACCA GACTGTCCGT 
ATCTGGAGTG CTTCTACAGG CAAAGCAGTT ACAGTGTTGA AGCTGCCATA CTTAAAAAGA 
CGTGGAGGCG GAGTGGATCC AGCCATCAAG GAGCGGCTCT GGTTGACACT GCACTGGCCA 
CCTGGCCATT CAACACAGAT TCTGTCCAGT TGTTTTGGGG GTGAATTGCT CCTGTGGGAT 
TTAACCAAAT CTGGGAAGCA GAAGTGGACT CTCCTTGGCA CCTCTGATGG ACAGAATCAT 
TCTCGAATTG TCTTTAACCT TTGTTCTGTG GTCACTGAGG AAGGGAAAGA GCTCTTGCTG 
TCTACTTCCA TGGACCGAGA TGTGAAATGT TGGGATCTGG GTTCCCTGTC CTGCTGCTGG 
TCCCTAACTT CCCTCGGTGG CTTTGTATAC AGCCTAGCTT TCTCCCCGGT AGCAGCTGGC 
AGCCTGGCTG TTGGGGTGGG GGACAGCATG ATCCGTGTAT GGAATACAAT GTCGGTTGTC 
AATGCATATG ATGTGAAGAC CCTGTGGCAG GGCATCAGAT CCAAGGTCAC TGCGCTGTCA 
TGGCATCCGA CCAAAGAGGG CAGTCTTGCT TTTGGCACCG AGGATGGGAA AGTTGGCATC 
TTTGAGGTCT ACATCAGCAA ACCCCCTCAG ATTTCTGCTA CATACCACAA AAAGACTGTT 
TATTCCTTAT CATGGGGACC TCTGCTGCCT CCATTCTCAT TTGGAGCAGA AGGGGACCGT 
CCTACTTATA CACTGTACAG CTGTGGGGGG GAAGGGGTTA TTTACCAGCA CAATCCTTGG 
AAACTGACAG ATGAAGCCAG TAATATTAAC AAGCTGATCC GGGACACCAA TGACATCAAA 
CACAAGTTAC CAGCACACAC AGAGTTAAGC TGGAGACCTG ATGGACAGTA TTTAGCTATT 
GGCAATGAAG ATGGAACCAT TGAGGTCTTC CACACTCAGC GTCTGCAGCT TATCTGTACC 
ATTCAGCAGC ATCACAAGCT AGTGAATGCT GTGCGCTGGC ATCATGGCCA TAGCCAAGAC 
CCTGACTTGA ACTTTCTGCT GGCATCTGGA TCTAACAATG CTACTGTCTA CGTGCACAAC 
CTGAAAGGGC CCCTGGAGAA TCCAACAGAT TCCCCAGTCA CCATCACTGA GCCTTTCCGT 
ACCTTGCCTG GACACACTGC AAAGATCACA AGCCTGTCAT GGAGCCCTCA CCACGATGCC 
AGACTGGTGT CTGCCAGTTA TGATGGTACT GCCCAGGTAT GGGATGTTCT GCAGGAAGAG 
CCACTCAGTA ACTATCGTGG GCACAAAGGA AGACTGCTTT CTGTTCAGTG GTCTCCTGTG 
GAGCCAGACC AGGTGTGGAC AGGAGCTGAT GACTTTTGCC TTCATAGCTG GTCTGTATCA 
AAGCAAGAGC ATAGCAGGCC TCCCAAAGGG AAAAAAGCCA CTGAGCTTGA AAAAAAGCGT 
ACCCTTCAAC CAAAACCAAA AGCCAAGAAG AAGAAAAAGT TAGACACCCC CAAAGAGGAG 
GCAGGAAGAG AGGTACAGAG GGGAGAAAAG GAAAGCCCTC TAACACAGAA TGGGACATCC 
GATCAAGAGG AGGGACAGGG GACTCCTGAG CCAGAGGCAG CTGACAGAGG CTTACATGAA 
GATCTGCAAA TCAGGAAGAA GAAGAGTCGA TCGTTGCTTC CCATCAGCAC CAGCAAAGAT 
CACCGATCTA AAGAAGAGCA ACACCGAGAC TGTGTTAAGT TGGCAACTGT TGTATGTGCG 
AAGGATTTGG AAGAGAAATC TCATTTTCAC TCGGAGGACA CAATGCACCT GGGACTATAC 
AGTGACCGAG CAGCCCTTTA TCACCTTATG GAGGAGGAGG GTAAGGCTCA TCTGGAGAAT 
GGGCACCCTG AGCTCCAGCA TCAAATTATG CTTTGGAAAG GGGATCTGAA GGGGACTTTG 
CAACTGGCTG CAGAGAGGGG TGAACTGACT GATCAGCTGG TGGCGCTGTC TCCCGTAGCC 
GGATACAAGG TCTGGCTATG GACCGTTGAA GCCTTTGTGA AACAGCTGTG CTTTCATGAG 
CAGTACGTGA AAGCCGCTTC CCACCTGCTG TCCATTAATA AAGTGTATGA AGCCGTGGAA 
CTGCTCAAGG GGAACCACCT CTACAGAGAA GCCATTGCAG TGGCGAAGGC TCGGCTACTC 
CCCGATGATT CAGTCCTGCG GGAGCTGTAC ACTAGCTGGG CAGCAGTCCT TGAGCAGGAC 
GGGCACTATT CCATGGCTGC TAAGTGTTAT TTAGGGTCCT CCTCTCCCTA TGATGCTGCC 
AAGGTTCTAG CTAAGAAGGG GGATTTGCTA TCCCTGAGCG CTGCTGCTGA ACTTGCATTA 
GTTGCAGGAG AAAAAGACCT TGCTGCTTCT TTTGCCATCC GCTGCGCCCA GGAACTGTTC 
ACTGCAAAGA ATTTTTTAGC AGCCCAGGAA GCACTACGAC AGCACAACAG TCTGCTGGGG 
CAGAGACTGC TGTTTTGTAC CAGTGAGCTG CTGTACATGC ATTTGAAGGA GAAGATGCCC 
ATAGATTGGA AGACACAAAG CAATCCATGT TTCCATGAAC AGACAGGCAG TAGTAATGGG 
GAACCTTTTA TGACCAAGCT TACAAGTCTA TGGCAGAGTT TGTGTGAAGT AAACAGCCCA 
GAGCAATACA AAGATGCCTT GCAACAACTG TCTGCCTCAG AGATTCCACA GACCACTGTC 
AATACTGGCC CTAAACAGCT GCTGCTGCAC CTTTCCCATG AGGTCACAAT ATTGTCTCTG 
AATTGTCAGC TTAAAAACTG GAAAGAGGCT GTTCCATCTC TCCTCCATAT CCTGTTCCGC 
TGTCAGCAGG CAGGCCATTT CACCCTGATG CAAGGGATAG CCAGCCTGCT GCTTCCTAAC 
GGCTGTGAAG AGCTGAGGGC TCTGTTGTCC CCAGAAAACA AAGAGAATTT GACTGCCTGT 
GACAGCGTGC AGGCCTTCCT TGCTTACATA AAGATGTATG AGCTGTGGTG GGGACAGCAA 
GAAATGCCAC CAAATTCCAA AGATCCAGCG GCTGCAGTAA ATATGGACAG TCCAAATGGA 
GCCTCTGCTG CTGTGCAGGA GCCAGATATG AAACCAGGGG ATTTGTGCGG CTTTCAGGTG 
TTGCTGTCAG ACCTTCACGC GCCCTTCCAG AGTGCCCAAA GAGAGATTGC TGAGGTTCAG 
AGAGCCCTTC GGGAGATGAT ACAGCAACAC CAGAGAATCA GGCAGAACCA GGACAGCTCT 
TTGGAACTAG AACAAGAGCC TGAATTAAAC ACTTCTTCAT CTCAGGATAA ATCTCTTGAC 
AGCCAAGTCA GCATATCCGC TGGTCCAAGT CCTGTGTCAC TGACAGAGCT GATGAAGAAG 
CTGTCAGTGG CCAACCACCG ACTAGCAGAG TTCCCTGAAT ATTTAAAGGC CTTACCCTTT 
CCTGATGTAC TAGAGAGCTG CCTGGTGCTA CTGCATCTTC CATCCTATCA GATCTCCCCA 
GAGATCCGCT CCCAAGCCTT GGCGTTGTTG CACAGTCATA GCCATGGATC TGCTGATCAC 
CAAATAGCAG CCAAGAAGTT CCTCTCCATC CCAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gemin5 ( XM_004912787.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912844.1 Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa07510
Clone ID Related Accession (Same CDS sequence)	XM_004912787.1 , XM_004912786.1
Accession Version	XM_004912787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gem-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAC CAGATCGGAT GCTCAGTGCG TCACCCAACT GGTACTGTAG CCGCTCCAGT 
GATGCTGCCG GTGGACTGTT CGGATTCGCT GCTCGAAATT CTGTCTACCT CCTGAACATT 
AATCTGCAGG AGCCTGGATT TTATGGAGAG CTGGTTGGAC ATACAGACCG CGTCTCTGGC 
TTTGCTTTTT CCCATTTCTC AGGCCAATCT CACCTGTGTG CCAGTAGCTC TGATGATGGT 
ACTGTAAAGG TTTGGGACAC GCAGACAAAG AACACTGTAA CGGAGCACCA TCTCCATCGG 
AGTACAATTG CTGCTCTTCA CTGGTCACCT TTAGTGAAGG ATCTGCTGGT ATCCGGGGAT 
GAAAAAGGAG TATTGGTTTG TTTCTGGTGT AACCGAAATG ACAGCCAGCA GTTTTTTCCA 
GAGCCAAGGA CTATTTTCTG CCTTTCCTGC TCCCCGCACA AGGAGGACAT TGTAGCTATA 
GGGTACAAAG ATGGCATGAT AGTGCTGGTG GATATCAGCC GCAGAGGAGA TGTGTTGCAT 
CGGCTACGAG GGCATGAAGA GGAGATACAC TGTTTGGAAT GGAGCCCCCA TGCCTTGGAG 
TGTGACCTGC AACAAAAGCT GGAGGAGACA GAGATAAACA CAGCCAATGG GGAGCTTCTT 
CCTGAGACAG AAAACAGGAG ATGTTATCTG GCTTCTGGCA GCAAAGACCA GACTGTCCGT 
ATCTGGAGTG CTTCTACAGG CAAAGCAGTT ACAGTGTTGA AGCTGCCATA CTTAAAAAGA 
CGTGGAGGCG GAGTGGATCC AGCCATCAAG GAGCGGCTCT GGTTGACACT GCACTGGCCA 
CCTGGCCATT CAACACAGAT TCTGTCCAGT TGTTTTGGGG GTGAATTGCT CCTGTGGGAT 
TTAACCAAAT CTGGGAAGCA GAAGTGGACT CTCCTTGGCA CCTCTGATGG ACAGAATCAT 
TCTCGAATTG TCTTTAACCT TTGTTCTGTG GTCACTGAGG AAGGGAAAGA GCTCTTGCTG 
TCTACTTCCA TGGACCGAGA TGTGAAATGT TGGGATCTGG GTTCCCTGTC CTGCTGCTGG 
TCCCTAACTT CCCTCGGTGG CTTTGTATAC AGCCTAGCTT TCTCCCCGGT AGCAGCTGGC 
AGCCTGGCTG TTGGGGTGGG GGACAGCATG ATCCGTGTAT GGAATACAAT GTCGGTTGTC 
AATGCATATG ATGTGAAGAC CCTGTGGCAG GGCATCAGAT CCAAGGTCAC TGCGCTGTCA 
TGGCATCCGA CCAAAGAGGG CAGTCTTGCT TTTGGCACCG AGGATGGGAA AGTTGGCATC 
TTTGAGGTCT ACATCAGCAA ACCCCCTCAG ATTTCTGCTA CATACCACAA AAAGACTGTT 
TATTCCTTAT CATGGGGACC TCTGCTGCCT CCATTCTCAT TTGGAGCAGA AGGGGACCGT 
CCTACTTATA CACTGTACAG CTGTGGGGGG GAAGGGGTTA TTTACCAGCA CAATCCTTGG 
AAACTGACAG ATGAAGCCAG TAATATTAAC AAGCTGATCC GGGACACCAA TGACATCAAA 
CACAAGTTAC CAGCACACAC AGAGTTAAGC TGGAGACCTG ATGGACAGTA TTTAGCTATT 
GGCAATGAAG ATGGAACCAT TGAGGTCTTC CACACTCAGC GTCTGCAGCT TATCTGTACC 
ATTCAGCAGC ATCACAAGCT AGTGAATGCT GTGCGCTGGC ATCATGGCCA TAGCCAAGAC 
CCTGACTTGA ACTTTCTGCT GGCATCTGGA TCTAACAATG CTACTGTCTA CGTGCACAAC 
CTGAAAGGGC CCCTGGAGAA TCCCACAGAT TCCCCAGTCA CCATCACTGA GCCTTTCCGT 
ACCTTGCCTG GACACACTGC AAAGATCACA AGCCTGTCAT GGAGCCCTCA CCACGATGCC 
AGACTGGTGT CTGCCAGTTA TGATGGTACT GCCCAGGTAT GGGATGTTCT GCAGGAAGAG 
CCACTCAGTA ACTATCGTGG GCACAAAGGA AGACTGCTTT CTGTTCAGTG GTCTCCTGTG 
GAGCCAGACC AGGTGTGGAC AGGAGCTGAT GACTTTTGCC TTCATAGCTG GTCTGTATCA 
AAGCAAGAGC ATAGCAGGCC TCCCAAAGGG AAAAAAGCCA CTGAGCTTGA AAAAAAGCGT 
ACCCTTCAAC CAAAACCAAA AGCCAAGAAG AAGAAAAAGT TAGACACCCC CAAAGAGGAG 
GCAGGAAGAG AGGTACAGAG GGGAGAAAAG GAAAGCCCTC TAACACAGAA TGGGACATCC 
GATCAAGAGG AGGGACAGGG GACTCCTGAG CCAGAGGCAG CTGACAGAGG CTTACATGAA 
GCAGATGTAG AATGTCCTTT TCAGCCAGTG GTCCCTTCTC CAGTTCCTGC TCAGGACAAG 
CACAGTCCCA TTTCATTTGC TAAGACTGTA CCAGTTAGGA AGGACCTGGC CAAAGAACGA 
GCAGATCTGC AAATCAGGAA GAAGAAGAGT CGATCGTTGC TTCCCATCAG CACCAGCAAA 
GATCACCGAT CTAAAGAAGA GCAACACCGA GACTGTGTTA AGTTGGCAAC TGTTGTATGT 
GCGAAGGATT TGGAAGAGAA ATCTCATTTT CACTCGGAGG ACACAATGCA CCTGGGACTA 
TACAGTGACC GAGCAGCCCT TTATCACCTT ATGGAGGAGG AGGGTAAGGC TCATCTGGAG 
AATGGGCACC CTGAGCTCCA GCATCAAATT ATGCTTTGGA AAGGGGATCT GAAGGGGACT 
TTGCAACTGG CTGCAGAGAG GGGTGAACTG ACTGATCAGC TGGTGGCGCT GTCTCCCGTA 
GCCGGATACA AGGTCTGGCT ATGGACCGTT GAAGCCTTTG TGAAACAGCT GTGCTTTCAT 
GAGCAGTACG TGAAAGCCGC TTCCCACCTG CTGTCCATTA ATAAAGTGTA TGAAGCCGTG 
GAACTGCTCA AGGGGAACCA CCTCTACAGA GAAGCCATTG CAGTGGCGAA GGCTCGGCTA 
CTCCCCGATG ATTCAGTCCT GCGGGAGCTG TACACTAGCT GGGCAGCAGT CCTTGAGCAG 
GACGGGCACT ATTCCATGGC TGCTAAGTGT TATTTAGGGT CCTCCTCTCC CTATGATGCT 
GCCAAGGTTC TAGCTAAGAA GGGGGATTTG CTATCCCTGA GCGCTGCTGC TGAACTTGCA 
TTAGTTGCAG GAGAAAAAGA CCTTGCTGCT TCTTTTGCCA TCCGCTGCGC CCAGGAACTG 
TTCACTGCAA AGAATTTTTT AGCAGCCCAG GAAGCACTAC GACAGCACAA CAGTCTGCTG 
GGGCAGAGAC TGCTGTTTTG TACCAGTGAG CTGCTGTACA TGCATTTGAA GGAGAAGATG 
CCCATAGATT GGAAGACACA AAGCAATCCA TGTTTCCATG AACAGACAGG CAGTAGTAAT 
GGGGAACCTT TTATGACCAA GCTTACAAGT CTATGGCAGA GTTTGTGTGA AGTAAACAGC 
CCAGAGCAAT ACAAAGATGC CTTGCAACAA CTGTCTGCCT CAGAGATTCC ACAGACCACT 
GTCAATACTG GCCCTAAACA GCTGCTGCTG CACCTTTCCC ATGAGGTCAC AATATTGTCT 
CTGAATTGTC AGCTTAAAAA CTGGAAAGAG GCTGTTCCAT CTCTCCTCCA TATCCTGTTC 
CGCTGTCAGC AGGCAGGCCA TTTCACCCTG ATGCAAGGGA TAGCCAGCCT GCTGCTTCCT 
AACGGCTGTG AAGAGCTGAG GGCTCTGTTG TCCCCAGAAA ACAAAGAGAA TTTGACTGCC 
TGTGACAGCG TGCAGGCCTT CCTTGCTTAC ATAAAGATGT ATGAGCTGTG GTGGGGACAG 
CAAGAAATGC CACCAAATTC CAAAGATCCA GCGGCTGCAG TAAATATGGA CAGTCCAAAT 
GGAGCCTCTG CTGCTGTGCA GGAGCCAGAT ATGAAACCAG GGGATTTGTG CGGCTTTCAG 
GTGTTGCTGT CAGACCTTCA CGCGCCCTTC CAGAGTGCCC AAAGAGAGAT TGCTGAGGTT 
CAGAGAGCCC TTCGGGAGAT GATACAGCAA CACCAGAGAA TCAGGCAGAA CCAGGACAGC 
TCTTTGGAAC TAGAACAAGA GCCTGAATTA AACACTTCTT CATCTCAGGA TAAATCTCTT 
GACAGCCAAG TCAGCATATC CGCTGGTCCA AGTCCTGTGT CACTGACAGA GCTGATGAAG 
AAGCTGTCAG TGGCCAACCA CCGACTAGCA GAGTTCCCTG AATATTTAAA GGCCTTACCC 
TTTCCTGATG TACTAGAGAG CTGCCTGGTG CTACTGCATC TTCCATCCTA TCAGATCTCC 
CCAGAGATCC GCTCCCAAGC CTTGGCGTTG TTGCACAGTC ATAGCCATGG ATCTGCTGAT 
CACCAAATAG CAGCCAAGAA GTTCCTCTCC ATCCCAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912844.1
CDS	92..4513
Translation	MGEPDRMLSASPNWYCSRSSDAAGGLFGFAARNSVYLLNINLQEPGFYGELVGHTDRVSGFAFSHFSGQSHLCASSSDDGTVKVWDTQTKNTVTEHHLHRSTIAALHWSPLVKDLLVSGDEKGVLVCFWCNRNDSQQFFPEPRTIFCLSCSPHKEDIVAIGYKDGMIVLVDISRRGDVLHRLRGHEEEIHCLEWSPHALECDLQQKLEETEINTANGELLPETENRRCYLASGSKDQTVRIWSASTGKAVTVLKLPYLKRRGGGVDPAIKERLWLTLHWPPGHSTQILSSCFGGELLLWDLTKSGKQKWTLLGTSDGQNHSRIVFNLCSVVTEEGKELLLSTSMDRDVKCWDLGSLSCCWSLTSLGGFVYSLAFSPVAAGSLAVGVGDSMIRVWNTMSVVNAYDVKTLWQGIRSKVTALSWHPTKEGSLAFGTEDGKVGIFEVYISKPPQISATYHKKTVYSLSWGPLLPPFSFGAEGDRPTYTLYSCGGEGVIYQHNPWKLTDEASNINKLIRDTNDIKHKLPAHTELSWRPDGQYLAIGNEDGTIEVFHTQRLQLICTIQQHHKLVNAVRWHHGHSQDPDLNFLLASGSNNATVYVHNLKGPLENPTDSPVTITEPFRTLPGHTAKITSLSWSPHHDARLVSASYDGTAQVWDVLQEEPLSNYRGHKGRLLSVQWSPVEPDQVWTGADDFCLHSWSVSKQEHSRPPKGKKATELEKKRTLQPKPKAKKKKKLDTPKEEAGREVQRGEKESPLTQNGTSDQEEGQGTPEPEAADRGLHEADVECPFQPVVPSPVPAQDKHSPISFAKTVPVRKDLAKERADLQIRKKKSRSLLPISTSKDHRSKEEQHRDCVKLATVVCAKDLEEKSHFHSEDTMHLGLYSDRAALYHLMEEEGKAHLENGHPELQHQIMLWKGDLKGTLQLAAERGELTDQLVALSPVAGYKVWLWTVEAFVKQLCFHEQYVKAASHLLSINKVYEAVELLKGNHLYREAIAVAKARLLPDDSVLRELYTSWAAVLEQDGHYSMAAKCYLGSSSPYDAAKVLAKKGDLLSLSAAAELALVAGEKDLAASFAIRCAQELFTAKNFLAAQEALRQHNSLLGQRLLFCTSELLYMHLKEKMPIDWKTQSNPCFHEQTGSSNGEPFMTKLTSLWQSLCEVNSPEQYKDALQQLSASEIPQTTVNTGPKQLLLHLSHEVTILSLNCQLKNWKEAVPSLLHILFRCQQAGHFTLMQGIASLLLPNGCEELRALLSPENKENLTACDSVQAFLAYIKMYELWWGQQEMPPNSKDPAAAVNMDSPNGASAAVQEPDMKPGDLCGFQVLLSDLHAPFQSAQREIAEVQRALREMIQQHQRIRQNQDSSLELEQEPELNTSSSQDKSLDSQVSISAGPSPVSLTELMKKLSVANHRLAEFPEYLKALPFPDVLESCLVLLHLPSYQISPEIRSQALALLHSHSHGSADHQIAAKKFLSIPE

Target ORF information:

RefSeq Version	XM_004912787.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912787.1

ORF Insert Sequence:

ATGGGGGAAC CAGATCGGAT GCTCAGTGCG TCACCCAACT GGTACTGTAG CCGCTCCAGT 
GATGCTGCCG GTGGACTGTT CGGATTCGCT GCTCGAAATT CTGTCTACCT CCTGAACATT 
AATCTGCAGG AGCCTGGATT TTATGGAGAG CTGGTTGGAC ATACAGACCG CGTCTCTGGC 
TTTGCTTTTT CCCATTTCTC AGGCCAATCT CACCTGTGTG CCAGTAGCTC TGATGATGGT 
ACTGTAAAGG TTTGGGACAC GCAGACAAAG AACACTGTAA CGGAGCACCA TCTCCATCGG 
AGTACAATTG CTGCTCTTCA CTGGTCACCT TTAGTGAAGG ATCTGCTGGT ATCCGGGGAT 
GAAAAAGGAG TATTGGTTTG TTTCTGGTGT AACCGAAATG ACAGCCAGCA GTTTTTTCCA 
GAGCCAAGGA CTATTTTCTG CCTTTCCTGC TCCCCGCACA AGGAGGACAT TGTAGCTATA 
GGGTACAAAG ATGGCATGAT AGTGCTGGTG GATATCAGCC GCAGAGGAGA TGTGTTGCAT 
CGGCTACGAG GGCATGAAGA GGAGATACAC TGTTTGGAAT GGAGCCCCCA TGCCTTGGAG 
TGTGACCTGC AACAAAAGCT GGAGGAGACA GAGATAAACA CAGCCAATGG GGAGCTTCTT 
CCTGAGACAG AAAACAGGAG ATGTTATCTG GCTTCTGGCA GCAAAGACCA GACTGTCCGT 
ATCTGGAGTG CTTCTACAGG CAAAGCAGTT ACAGTGTTGA AGCTGCCATA CTTAAAAAGA 
CGTGGAGGCG GAGTGGATCC AGCCATCAAG GAGCGGCTCT GGTTGACACT GCACTGGCCA 
CCTGGCCATT CAACACAGAT TCTGTCCAGT TGTTTTGGGG GTGAATTGCT CCTGTGGGAT 
TTAACCAAAT CTGGGAAGCA GAAGTGGACT CTCCTTGGCA CCTCTGATGG ACAGAATCAT 
TCTCGAATTG TCTTTAACCT TTGTTCTGTG GTCACTGAGG AAGGGAAAGA GCTCTTGCTG 
TCTACTTCCA TGGACCGAGA TGTGAAATGT TGGGATCTGG GTTCCCTGTC CTGCTGCTGG 
TCCCTAACTT CCCTCGGTGG CTTTGTATAC AGCCTAGCTT TCTCCCCGGT AGCAGCTGGC 
AGCCTGGCTG TTGGGGTGGG GGACAGCATG ATCCGTGTAT GGAATACAAT GTCGGTTGTC 
AATGCATATG ATGTGAAGAC CCTGTGGCAG GGCATCAGAT CCAAGGTCAC TGCGCTGTCA 
TGGCATCCGA CCAAAGAGGG CAGTCTTGCT TTTGGCACCG AGGATGGGAA AGTTGGCATC 
TTTGAGGTCT ACATCAGCAA ACCCCCTCAG ATTTCTGCTA CATACCACAA AAAGACTGTT 
TATTCCTTAT CATGGGGACC TCTGCTGCCT CCATTCTCAT TTGGAGCAGA AGGGGACCGT 
CCTACTTATA CACTGTACAG CTGTGGGGGG GAAGGGGTTA TTTACCAGCA CAATCCTTGG 
AAACTGACAG ATGAAGCCAG TAATATTAAC AAGCTGATCC GGGACACCAA TGACATCAAA 
CACAAGTTAC CAGCACACAC AGAGTTAAGC TGGAGACCTG ATGGACAGTA TTTAGCTATT 
GGCAATGAAG ATGGAACCAT TGAGGTCTTC CACACTCAGC GTCTGCAGCT TATCTGTACC 
ATTCAGCAGC ATCACAAGCT AGTGAATGCT GTGCGCTGGC ATCATGGCCA TAGCCAAGAC 
CCTGACTTGA ACTTTCTGCT GGCATCTGGA TCTAACAATG CTACTGTCTA CGTGCACAAC 
CTGAAAGGGC CCCTGGAGAA TCCCACAGAT TCCCCAGTCA CCATCACTGA GCCTTTCCGT 
ACCTTGCCTG GACACACTGC AAAGATCACA AGCCTGTCAT GGAGCCCTCA CCACGATGCC 
AGACTGGTGT CTGCCAGTTA TGATGGTACT GCCCAGGTAT GGGATGTTCT GCAGGAAGAG 
CCACTCAGTA ACTATCGTGG GCACAAAGGA AGACTGCTTT CTGTTCAGTG GTCTCCTGTG 
GAGCCAGACC AGGTGTGGAC AGGAGCTGAT GACTTTTGCC TTCATAGCTG GTCTGTATCA 
AAGCAAGAGC ATAGCAGGCC TCCCAAAGGG AAAAAAGCCA CTGAGCTTGA AAAAAAGCGT 
ACCCTTCAAC CAAAACCAAA AGCCAAGAAG AAGAAAAAGT TAGACACCCC CAAAGAGGAG 
GCAGGAAGAG AGGTACAGAG GGGAGAAAAG GAAAGCCCTC TAACACAGAA TGGGACATCC 
GATCAAGAGG AGGGACAGGG GACTCCTGAG CCAGAGGCAG CTGACAGAGG CTTACATGAA 
GCAGATGTAG AATGTCCTTT TCAGCCAGTG GTCCCTTCTC CAGTTCCTGC TCAGGACAAG 
CACAGTCCCA TTTCATTTGC TAAGACTGTA CCAGTTAGGA AGGACCTGGC CAAAGAACGA 
GCAGATCTGC AAATCAGGAA GAAGAAGAGT CGATCGTTGC TTCCCATCAG CACCAGCAAA 
GATCACCGAT CTAAAGAAGA GCAACACCGA GACTGTGTTA AGTTGGCAAC TGTTGTATGT 
GCGAAGGATT TGGAAGAGAA ATCTCATTTT CACTCGGAGG ACACAATGCA CCTGGGACTA 
TACAGTGACC GAGCAGCCCT TTATCACCTT ATGGAGGAGG AGGGTAAGGC TCATCTGGAG 
AATGGGCACC CTGAGCTCCA GCATCAAATT ATGCTTTGGA AAGGGGATCT GAAGGGGACT 
TTGCAACTGG CTGCAGAGAG GGGTGAACTG ACTGATCAGC TGGTGGCGCT GTCTCCCGTA 
GCCGGATACA AGGTCTGGCT ATGGACCGTT GAAGCCTTTG TGAAACAGCT GTGCTTTCAT 
GAGCAGTACG TGAAAGCCGC TTCCCACCTG CTGTCCATTA ATAAAGTGTA TGAAGCCGTG 
GAACTGCTCA AGGGGAACCA CCTCTACAGA GAAGCCATTG CAGTGGCGAA GGCTCGGCTA 
CTCCCCGATG ATTCAGTCCT GCGGGAGCTG TACACTAGCT GGGCAGCAGT CCTTGAGCAG 
GACGGGCACT ATTCCATGGC TGCTAAGTGT TATTTAGGGT CCTCCTCTCC CTATGATGCT 
GCCAAGGTTC TAGCTAAGAA GGGGGATTTG CTATCCCTGA GCGCTGCTGC TGAACTTGCA 
TTAGTTGCAG GAGAAAAAGA CCTTGCTGCT TCTTTTGCCA TCCGCTGCGC CCAGGAACTG 
TTCACTGCAA AGAATTTTTT AGCAGCCCAG GAAGCACTAC GACAGCACAA CAGTCTGCTG 
GGGCAGAGAC TGCTGTTTTG TACCAGTGAG CTGCTGTACA TGCATTTGAA GGAGAAGATG 
CCCATAGATT GGAAGACACA AAGCAATCCA TGTTTCCATG AACAGACAGG CAGTAGTAAT 
GGGGAACCTT TTATGACCAA GCTTACAAGT CTATGGCAGA GTTTGTGTGA AGTAAACAGC 
CCAGAGCAAT ACAAAGATGC CTTGCAACAA CTGTCTGCCT CAGAGATTCC ACAGACCACT 
GTCAATACTG GCCCTAAACA GCTGCTGCTG CACCTTTCCC ATGAGGTCAC AATATTGTCT 
CTGAATTGTC AGCTTAAAAA CTGGAAAGAG GCTGTTCCAT CTCTCCTCCA TATCCTGTTC 
CGCTGTCAGC AGGCAGGCCA TTTCACCCTG ATGCAAGGGA TAGCCAGCCT GCTGCTTCCT 
AACGGCTGTG AAGAGCTGAG GGCTCTGTTG TCCCCAGAAA ACAAAGAGAA TTTGACTGCC 
TGTGACAGCG TGCAGGCCTT CCTTGCTTAC ATAAAGATGT ATGAGCTGTG GTGGGGACAG 
CAAGAAATGC CACCAAATTC CAAAGATCCA GCGGCTGCAG TAAATATGGA CAGTCCAAAT 
GGAGCCTCTG CTGCTGTGCA GGAGCCAGAT ATGAAACCAG GGGATTTGTG CGGCTTTCAG 
GTGTTGCTGT CAGACCTTCA CGCGCCCTTC CAGAGTGCCC AAAGAGAGAT TGCTGAGGTT 
CAGAGAGCCC TTCGGGAGAT GATACAGCAA CACCAGAGAA TCAGGCAGAA CCAGGACAGC 
TCTTTGGAAC TAGAACAAGA GCCTGAATTA AACACTTCTT CATCTCAGGA TAAATCTCTT 
GACAGCCAAG TCAGCATATC CGCTGGTCCA AGTCCTGTGT CACTGACAGA GCTGATGAAG 
AAGCTGTCAG TGGCCAACCA CCGACTAGCA GAGTTCCCTG AATATTTAAA GGCCTTACCC 
TTTCCTGATG TACTAGAGAG CTGCCTGGTG CTACTGCATC TTCCATCCTA TCAGATCTCC 
CCAGAGATCC GCTCCCAAGC CTTGGCGTTG TTGCACAGTC ATAGCCATGG ATCTGCTGAT 
CACCAAATAG CAGCCAAGAA GTTCCTCTCC ATCCCAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gemin5 ( XM_004912786.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912843.1 Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X1, mRNA.

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CloneID	OXa07510
Clone ID Related Accession (Same CDS sequence)	XM_004912787.1 , XM_004912786.1
Accession Version	XM_004912786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gem-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAC CAGATCGGAT GCTCAGTGCG TCACCCAACT GGTACTGTAG CCGCTCCAGT 
GATGCTGCCG GTGGACTGTT CGGATTCGCT GCTCGAAATT CTGTCTACCT CCTGAACATT 
AATCTGCAGG AGCCTGGATT TTATGGAGAG CTGGTTGGAC ATACAGACCG CGTCTCTGGC 
TTTGCTTTTT CCCATTTCTC AGGCCAATCT CACCTGTGTG CCAGTAGCTC TGATGATGGT 
ACTGTAAAGG TTTGGGACAC GCAGACAAAG AACACTGTAA CGGAGCACCA TCTCCATCGG 
AGTACAATTG CTGCTCTTCA CTGGTCACCT TTAGTGAAGG ATCTGCTGGT ATCCGGGGAT 
GAAAAAGGAG TATTGGTTTG TTTCTGGTGT AACCGAAATG ACAGCCAGCA GTTTTTTCCA 
GAGCCAAGGA CTATTTTCTG CCTTTCCTGC TCCCCGCACA AGGAGGACAT TGTAGCTATA 
GGGTACAAAG ATGGCATGAT AGTGCTGGTG GATATCAGCC GCAGAGGAGA TGTGTTGCAT 
CGGCTACGAG GGCATGAAGA GGAGATACAC TGTTTGGAAT GGAGCCCCCA TGCCTTGGAG 
TGTGACCTGC AACAAAAGCT GGAGGAGACA GAGATAAACA CAGCCAATGG GGAGCTTCTT 
CCTGAGACAG AAAACAGGAG ATGTTATCTG GCTTCTGGCA GCAAAGACCA GACTGTCCGT 
ATCTGGAGTG CTTCTACAGG CAAAGCAGTT ACAGTGTTGA AGCTGCCATA CTTAAAAAGA 
CGTGGAGGCG GAGTGGATCC AGCCATCAAG GAGCGGCTCT GGTTGACACT GCACTGGCCA 
CCTGGCCATT CAACACAGAT TCTGTCCAGT TGTTTTGGGG GTGAATTGCT CCTGTGGGAT 
TTAACCAAAT CTGGGAAGCA GAAGTGGACT CTCCTTGGCA CCTCTGATGG ACAGAATCAT 
TCTCGAATTG TCTTTAACCT TTGTTCTGTG GTCACTGAGG AAGGGAAAGA GCTCTTGCTG 
TCTACTTCCA TGGACCGAGA TGTGAAATGT TGGGATCTGG GTTCCCTGTC CTGCTGCTGG 
TCCCTAACTT CCCTCGGTGG CTTTGTATAC AGCCTAGCTT TCTCCCCGGT AGCAGCTGGC 
AGCCTGGCTG TTGGGGTGGG GGACAGCATG ATCCGTGTAT GGAATACAAT GTCGGTTGTC 
AATGCATATG ATGTGAAGAC CCTGTGGCAG GGCATCAGAT CCAAGGTCAC TGCGCTGTCA 
TGGCATCCGA CCAAAGAGGG CAGTCTTGCT TTTGGCACCG AGGATGGGAA AGTTGGCATC 
TTTGAGGTCT ACATCAGCAA ACCCCCTCAG ATTTCTGCTA CATACCACAA AAAGACTGTT 
TATTCCTTAT CATGGGGACC TCTGCTGCCT CCATTCTCAT TTGGAGCAGA AGGGGACCGT 
CCTACTTATA CACTGTACAG CTGTGGGGGG GAAGGGGTTA TTTACCAGCA CAATCCTTGG 
AAACTGACAG ATGAAGCCAG TAATATTAAC AAGCTGATCC GGGACACCAA TGACATCAAA 
CACAAGTTAC CAGCACACAC AGAGTTAAGC TGGAGACCTG ATGGACAGTA TTTAGCTATT 
GGCAATGAAG ATGGAACCAT TGAGGTCTTC CACACTCAGC GTCTGCAGCT TATCTGTACC 
ATTCAGCAGC ATCACAAGCT AGTGAATGCT GTGCGCTGGC ATCATGGCCA TAGCCAAGAC 
CCTGACTTGA ACTTTCTGCT GGCATCTGGA TCTAACAATG CTACTGTCTA CGTGCACAAC 
CTGAAAGGGC CCCTGGAGAA TCCCACAGAT TCCCCAGTCA CCATCACTGA GCCTTTCCGT 
ACCTTGCCTG GACACACTGC AAAGATCACA AGCCTGTCAT GGAGCCCTCA CCACGATGCC 
AGACTGGTGT CTGCCAGTTA TGATGGTACT GCCCAGGTAT GGGATGTTCT GCAGGAAGAG 
CCACTCAGTA ACTATCGTGG GCACAAAGGA AGACTGCTTT CTGTTCAGTG GTCTCCTGTG 
GAGCCAGACC AGGTGTGGAC AGGAGCTGAT GACTTTTGCC TTCATAGCTG GTCTGTATCA 
AAGCAAGAGC ATAGCAGGCC TCCCAAAGGG AAAAAAGCCA CTGAGCTTGA AAAAAAGCGT 
ACCCTTCAAC CAAAACCAAA AGCCAAGAAG AAGAAAAAGT TAGACACCCC CAAAGAGGAG 
GCAGGAAGAG AGGTACAGAG GGGAGAAAAG GAAAGCCCTC TAACACAGAA TGGGACATCC 
GATCAAGAGG AGGGACAGGG GACTCCTGAG CCAGAGGCAG CTGACAGAGG CTTACATGAA 
GCAGATGTAG AATGTCCTTT TCAGCCAGTG GTCCCTTCTC CAGTTCCTGC TCAGGACAAG 
CACAGTCCCA TTTCATTTGC TAAGACTGTA CCAGTTAGGA AGGACCTGGC CAAAGAACGA 
GCAGATCTGC AAATCAGGAA GAAGAAGAGT CGATCGTTGC TTCCCATCAG CACCAGCAAA 
GATCACCGAT CTAAAGAAGA GCAACACCGA GACTGTGTTA AGTTGGCAAC TGTTGTATGT 
GCGAAGGATT TGGAAGAGAA ATCTCATTTT CACTCGGAGG ACACAATGCA CCTGGGACTA 
TACAGTGACC GAGCAGCCCT TTATCACCTT ATGGAGGAGG AGGGTAAGGC TCATCTGGAG 
AATGGGCACC CTGAGCTCCA GCATCAAATT ATGCTTTGGA AAGGGGATCT GAAGGGGACT 
TTGCAACTGG CTGCAGAGAG GGGTGAACTG ACTGATCAGC TGGTGGCGCT GTCTCCCGTA 
GCCGGATACA AGGTCTGGCT ATGGACCGTT GAAGCCTTTG TGAAACAGCT GTGCTTTCAT 
GAGCAGTACG TGAAAGCCGC TTCCCACCTG CTGTCCATTA ATAAAGTGTA TGAAGCCGTG 
GAACTGCTCA AGGGGAACCA CCTCTACAGA GAAGCCATTG CAGTGGCGAA GGCTCGGCTA 
CTCCCCGATG ATTCAGTCCT GCGGGAGCTG TACACTAGCT GGGCAGCAGT CCTTGAGCAG 
GACGGGCACT ATTCCATGGC TGCTAAGTGT TATTTAGGGT CCTCCTCTCC CTATGATGCT 
GCCAAGGTTC TAGCTAAGAA GGGGGATTTG CTATCCCTGA GCGCTGCTGC TGAACTTGCA 
TTAGTTGCAG GAGAAAAAGA CCTTGCTGCT TCTTTTGCCA TCCGCTGCGC CCAGGAACTG 
TTCACTGCAA AGAATTTTTT AGCAGCCCAG GAAGCACTAC GACAGCACAA CAGTCTGCTG 
GGGCAGAGAC TGCTGTTTTG TACCAGTGAG CTGCTGTACA TGCATTTGAA GGAGAAGATG 
CCCATAGATT GGAAGACACA AAGCAATCCA TGTTTCCATG AACAGACAGG CAGTAGTAAT 
GGGGAACCTT TTATGACCAA GCTTACAAGT CTATGGCAGA GTTTGTGTGA AGTAAACAGC 
CCAGAGCAAT ACAAAGATGC CTTGCAACAA CTGTCTGCCT CAGAGATTCC ACAGACCACT 
GTCAATACTG GCCCTAAACA GCTGCTGCTG CACCTTTCCC ATGAGGTCAC AATATTGTCT 
CTGAATTGTC AGCTTAAAAA CTGGAAAGAG GCTGTTCCAT CTCTCCTCCA TATCCTGTTC 
CGCTGTCAGC AGGCAGGCCA TTTCACCCTG ATGCAAGGGA TAGCCAGCCT GCTGCTTCCT 
AACGGCTGTG AAGAGCTGAG GGCTCTGTTG TCCCCAGAAA ACAAAGAGAA TTTGACTGCC 
TGTGACAGCG TGCAGGCCTT CCTTGCTTAC ATAAAGATGT ATGAGCTGTG GTGGGGACAG 
CAAGAAATGC CACCAAATTC CAAAGATCCA GCGGCTGCAG TAAATATGGA CAGTCCAAAT 
GGAGCCTCTG CTGCTGTGCA GGAGCCAGAT ATGAAACCAG GGGATTTGTG CGGCTTTCAG 
GTGTTGCTGT CAGACCTTCA CGCGCCCTTC CAGAGTGCCC AAAGAGAGAT TGCTGAGGTT 
CAGAGAGCCC TTCGGGAGAT GATACAGCAA CACCAGAGAA TCAGGCAGAA CCAGGACAGC 
TCTTTGGAAC TAGAACAAGA GCCTGAATTA AACACTTCTT CATCTCAGGA TAAATCTCTT 
GACAGCCAAG TCAGCATATC CGCTGGTCCA AGTCCTGTGT CACTGACAGA GCTGATGAAG 
AAGCTGTCAG TGGCCAACCA CCGACTAGCA GAGTTCCCTG AATATTTAAA GGCCTTACCC 
TTTCCTGATG TACTAGAGAG CTGCCTGGTG CTACTGCATC TTCCATCCTA TCAGATCTCC 
CCAGAGATCC GCTCCCAAGC CTTGGCGTTG TTGCACAGTC ATAGCCATGG ATCTGCTGAT 
CACCAAATAG CAGCCAAGAA GTTCCTCTCC ATCCCAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912843.1
CDS	99..4520
Translation	MGEPDRMLSASPNWYCSRSSDAAGGLFGFAARNSVYLLNINLQEPGFYGELVGHTDRVSGFAFSHFSGQSHLCASSSDDGTVKVWDTQTKNTVTEHHLHRSTIAALHWSPLVKDLLVSGDEKGVLVCFWCNRNDSQQFFPEPRTIFCLSCSPHKEDIVAIGYKDGMIVLVDISRRGDVLHRLRGHEEEIHCLEWSPHALECDLQQKLEETEINTANGELLPETENRRCYLASGSKDQTVRIWSASTGKAVTVLKLPYLKRRGGGVDPAIKERLWLTLHWPPGHSTQILSSCFGGELLLWDLTKSGKQKWTLLGTSDGQNHSRIVFNLCSVVTEEGKELLLSTSMDRDVKCWDLGSLSCCWSLTSLGGFVYSLAFSPVAAGSLAVGVGDSMIRVWNTMSVVNAYDVKTLWQGIRSKVTALSWHPTKEGSLAFGTEDGKVGIFEVYISKPPQISATYHKKTVYSLSWGPLLPPFSFGAEGDRPTYTLYSCGGEGVIYQHNPWKLTDEASNINKLIRDTNDIKHKLPAHTELSWRPDGQYLAIGNEDGTIEVFHTQRLQLICTIQQHHKLVNAVRWHHGHSQDPDLNFLLASGSNNATVYVHNLKGPLENPTDSPVTITEPFRTLPGHTAKITSLSWSPHHDARLVSASYDGTAQVWDVLQEEPLSNYRGHKGRLLSVQWSPVEPDQVWTGADDFCLHSWSVSKQEHSRPPKGKKATELEKKRTLQPKPKAKKKKKLDTPKEEAGREVQRGEKESPLTQNGTSDQEEGQGTPEPEAADRGLHEADVECPFQPVVPSPVPAQDKHSPISFAKTVPVRKDLAKERADLQIRKKKSRSLLPISTSKDHRSKEEQHRDCVKLATVVCAKDLEEKSHFHSEDTMHLGLYSDRAALYHLMEEEGKAHLENGHPELQHQIMLWKGDLKGTLQLAAERGELTDQLVALSPVAGYKVWLWTVEAFVKQLCFHEQYVKAASHLLSINKVYEAVELLKGNHLYREAIAVAKARLLPDDSVLRELYTSWAAVLEQDGHYSMAAKCYLGSSSPYDAAKVLAKKGDLLSLSAAAELALVAGEKDLAASFAIRCAQELFTAKNFLAAQEALRQHNSLLGQRLLFCTSELLYMHLKEKMPIDWKTQSNPCFHEQTGSSNGEPFMTKLTSLWQSLCEVNSPEQYKDALQQLSASEIPQTTVNTGPKQLLLHLSHEVTILSLNCQLKNWKEAVPSLLHILFRCQQAGHFTLMQGIASLLLPNGCEELRALLSPENKENLTACDSVQAFLAYIKMYELWWGQQEMPPNSKDPAAAVNMDSPNGASAAVQEPDMKPGDLCGFQVLLSDLHAPFQSAQREIAEVQRALREMIQQHQRIRQNQDSSLELEQEPELNTSSSQDKSLDSQVSISAGPSPVSLTELMKKLSVANHRLAEFPEYLKALPFPDVLESCLVLLHLPSYQISPEIRSQALALLHSHSHGSADHQIAAKKFLSIPE

Target ORF information:

RefSeq Version	XM_004912786.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912786.1

ORF Insert Sequence:

ATGGGGGAAC CAGATCGGAT GCTCAGTGCG TCACCCAACT GGTACTGTAG CCGCTCCAGT 
GATGCTGCCG GTGGACTGTT CGGATTCGCT GCTCGAAATT CTGTCTACCT CCTGAACATT 
AATCTGCAGG AGCCTGGATT TTATGGAGAG CTGGTTGGAC ATACAGACCG CGTCTCTGGC 
TTTGCTTTTT CCCATTTCTC AGGCCAATCT CACCTGTGTG CCAGTAGCTC TGATGATGGT 
ACTGTAAAGG TTTGGGACAC GCAGACAAAG AACACTGTAA CGGAGCACCA TCTCCATCGG 
AGTACAATTG CTGCTCTTCA CTGGTCACCT TTAGTGAAGG ATCTGCTGGT ATCCGGGGAT 
GAAAAAGGAG TATTGGTTTG TTTCTGGTGT AACCGAAATG ACAGCCAGCA GTTTTTTCCA 
GAGCCAAGGA CTATTTTCTG CCTTTCCTGC TCCCCGCACA AGGAGGACAT TGTAGCTATA 
GGGTACAAAG ATGGCATGAT AGTGCTGGTG GATATCAGCC GCAGAGGAGA TGTGTTGCAT 
CGGCTACGAG GGCATGAAGA GGAGATACAC TGTTTGGAAT GGAGCCCCCA TGCCTTGGAG 
TGTGACCTGC AACAAAAGCT GGAGGAGACA GAGATAAACA CAGCCAATGG GGAGCTTCTT 
CCTGAGACAG AAAACAGGAG ATGTTATCTG GCTTCTGGCA GCAAAGACCA GACTGTCCGT 
ATCTGGAGTG CTTCTACAGG CAAAGCAGTT ACAGTGTTGA AGCTGCCATA CTTAAAAAGA 
CGTGGAGGCG GAGTGGATCC AGCCATCAAG GAGCGGCTCT GGTTGACACT GCACTGGCCA 
CCTGGCCATT CAACACAGAT TCTGTCCAGT TGTTTTGGGG GTGAATTGCT CCTGTGGGAT 
TTAACCAAAT CTGGGAAGCA GAAGTGGACT CTCCTTGGCA CCTCTGATGG ACAGAATCAT 
TCTCGAATTG TCTTTAACCT TTGTTCTGTG GTCACTGAGG AAGGGAAAGA GCTCTTGCTG 
TCTACTTCCA TGGACCGAGA TGTGAAATGT TGGGATCTGG GTTCCCTGTC CTGCTGCTGG 
TCCCTAACTT CCCTCGGTGG CTTTGTATAC AGCCTAGCTT TCTCCCCGGT AGCAGCTGGC 
AGCCTGGCTG TTGGGGTGGG GGACAGCATG ATCCGTGTAT GGAATACAAT GTCGGTTGTC 
AATGCATATG ATGTGAAGAC CCTGTGGCAG GGCATCAGAT CCAAGGTCAC TGCGCTGTCA 
TGGCATCCGA CCAAAGAGGG CAGTCTTGCT TTTGGCACCG AGGATGGGAA AGTTGGCATC 
TTTGAGGTCT ACATCAGCAA ACCCCCTCAG ATTTCTGCTA CATACCACAA AAAGACTGTT 
TATTCCTTAT CATGGGGACC TCTGCTGCCT CCATTCTCAT TTGGAGCAGA AGGGGACCGT 
CCTACTTATA CACTGTACAG CTGTGGGGGG GAAGGGGTTA TTTACCAGCA CAATCCTTGG 
AAACTGACAG ATGAAGCCAG TAATATTAAC AAGCTGATCC GGGACACCAA TGACATCAAA 
CACAAGTTAC CAGCACACAC AGAGTTAAGC TGGAGACCTG ATGGACAGTA TTTAGCTATT 
GGCAATGAAG ATGGAACCAT TGAGGTCTTC CACACTCAGC GTCTGCAGCT TATCTGTACC 
ATTCAGCAGC ATCACAAGCT AGTGAATGCT GTGCGCTGGC ATCATGGCCA TAGCCAAGAC 
CCTGACTTGA ACTTTCTGCT GGCATCTGGA TCTAACAATG CTACTGTCTA CGTGCACAAC 
CTGAAAGGGC CCCTGGAGAA TCCCACAGAT TCCCCAGTCA CCATCACTGA GCCTTTCCGT 
ACCTTGCCTG GACACACTGC AAAGATCACA AGCCTGTCAT GGAGCCCTCA CCACGATGCC 
AGACTGGTGT CTGCCAGTTA TGATGGTACT GCCCAGGTAT GGGATGTTCT GCAGGAAGAG 
CCACTCAGTA ACTATCGTGG GCACAAAGGA AGACTGCTTT CTGTTCAGTG GTCTCCTGTG 
GAGCCAGACC AGGTGTGGAC AGGAGCTGAT GACTTTTGCC TTCATAGCTG GTCTGTATCA 
AAGCAAGAGC ATAGCAGGCC TCCCAAAGGG AAAAAAGCCA CTGAGCTTGA AAAAAAGCGT 
ACCCTTCAAC CAAAACCAAA AGCCAAGAAG AAGAAAAAGT TAGACACCCC CAAAGAGGAG 
GCAGGAAGAG AGGTACAGAG GGGAGAAAAG GAAAGCCCTC TAACACAGAA TGGGACATCC 
GATCAAGAGG AGGGACAGGG GACTCCTGAG CCAGAGGCAG CTGACAGAGG CTTACATGAA 
GCAGATGTAG AATGTCCTTT TCAGCCAGTG GTCCCTTCTC CAGTTCCTGC TCAGGACAAG 
CACAGTCCCA TTTCATTTGC TAAGACTGTA CCAGTTAGGA AGGACCTGGC CAAAGAACGA 
GCAGATCTGC AAATCAGGAA GAAGAAGAGT CGATCGTTGC TTCCCATCAG CACCAGCAAA 
GATCACCGAT CTAAAGAAGA GCAACACCGA GACTGTGTTA AGTTGGCAAC TGTTGTATGT 
GCGAAGGATT TGGAAGAGAA ATCTCATTTT CACTCGGAGG ACACAATGCA CCTGGGACTA 
TACAGTGACC GAGCAGCCCT TTATCACCTT ATGGAGGAGG AGGGTAAGGC TCATCTGGAG 
AATGGGCACC CTGAGCTCCA GCATCAAATT ATGCTTTGGA AAGGGGATCT GAAGGGGACT 
TTGCAACTGG CTGCAGAGAG GGGTGAACTG ACTGATCAGC TGGTGGCGCT GTCTCCCGTA 
GCCGGATACA AGGTCTGGCT ATGGACCGTT GAAGCCTTTG TGAAACAGCT GTGCTTTCAT 
GAGCAGTACG TGAAAGCCGC TTCCCACCTG CTGTCCATTA ATAAAGTGTA TGAAGCCGTG 
GAACTGCTCA AGGGGAACCA CCTCTACAGA GAAGCCATTG CAGTGGCGAA GGCTCGGCTA 
CTCCCCGATG ATTCAGTCCT GCGGGAGCTG TACACTAGCT GGGCAGCAGT CCTTGAGCAG 
GACGGGCACT ATTCCATGGC TGCTAAGTGT TATTTAGGGT CCTCCTCTCC CTATGATGCT 
GCCAAGGTTC TAGCTAAGAA GGGGGATTTG CTATCCCTGA GCGCTGCTGC TGAACTTGCA 
TTAGTTGCAG GAGAAAAAGA CCTTGCTGCT TCTTTTGCCA TCCGCTGCGC CCAGGAACTG 
TTCACTGCAA AGAATTTTTT AGCAGCCCAG GAAGCACTAC GACAGCACAA CAGTCTGCTG 
GGGCAGAGAC TGCTGTTTTG TACCAGTGAG CTGCTGTACA TGCATTTGAA GGAGAAGATG 
CCCATAGATT GGAAGACACA AAGCAATCCA TGTTTCCATG AACAGACAGG CAGTAGTAAT 
GGGGAACCTT TTATGACCAA GCTTACAAGT CTATGGCAGA GTTTGTGTGA AGTAAACAGC 
CCAGAGCAAT ACAAAGATGC CTTGCAACAA CTGTCTGCCT CAGAGATTCC ACAGACCACT 
GTCAATACTG GCCCTAAACA GCTGCTGCTG CACCTTTCCC ATGAGGTCAC AATATTGTCT 
CTGAATTGTC AGCTTAAAAA CTGGAAAGAG GCTGTTCCAT CTCTCCTCCA TATCCTGTTC 
CGCTGTCAGC AGGCAGGCCA TTTCACCCTG ATGCAAGGGA TAGCCAGCCT GCTGCTTCCT 
AACGGCTGTG AAGAGCTGAG GGCTCTGTTG TCCCCAGAAA ACAAAGAGAA TTTGACTGCC 
TGTGACAGCG TGCAGGCCTT CCTTGCTTAC ATAAAGATGT ATGAGCTGTG GTGGGGACAG 
CAAGAAATGC CACCAAATTC CAAAGATCCA GCGGCTGCAG TAAATATGGA CAGTCCAAAT 
GGAGCCTCTG CTGCTGTGCA GGAGCCAGAT ATGAAACCAG GGGATTTGTG CGGCTTTCAG 
GTGTTGCTGT CAGACCTTCA CGCGCCCTTC CAGAGTGCCC AAAGAGAGAT TGCTGAGGTT 
CAGAGAGCCC TTCGGGAGAT GATACAGCAA CACCAGAGAA TCAGGCAGAA CCAGGACAGC 
TCTTTGGAAC TAGAACAAGA GCCTGAATTA AACACTTCTT CATCTCAGGA TAAATCTCTT 
GACAGCCAAG TCAGCATATC CGCTGGTCCA AGTCCTGTGT CACTGACAGA GCTGATGAAG 
AAGCTGTCAG TGGCCAACCA CCGACTAGCA GAGTTCCCTG AATATTTAAA GGCCTTACCC 
TTTCCTGATG TACTAGAGAG CTGCCTGGTG CTACTGCATC TTCCATCCTA TCAGATCTCC 
CCAGAGATCC GCTCCCAAGC CTTGGCGTTG TTGCACAGTC ATAGCCATGG ATCTGCTGAT 
CACCAAATAG CAGCCAAGAA GTTCCTCTCC ATCCCAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gemin5 ( XM_002932376.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932422.1 Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X3, mRNA.

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CloneID	OXa07511
Clone ID Related Accession (Same CDS sequence)	XM_002932376.2
Accession Version	XM_002932376.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gem-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 18, 2013 this sequence version replaced XM_002932376.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAC CAGATCGGAT GCTCAGTGCG TCACCCAACT GGTACTGTAG CCGCTCCAGT 
GATGCTGCCG GTGGACTGTT CGGATTCGCT GCTCGAAATT CTGTCTACCT CCTGAACATT 
AATCTGCAGG AGCCTGGATT TTATGGAGAG CTGGTTGGAC ATACAGACCG CGTCTCTGGC 
TTTGCTTTTT CCCATTTCTC AGGCCAATCT CACCTGTGTG CCAGTAGCTC TGATGATGGT 
ACTGTAAAGG TTTGGGACAC GCAGACAAAG AACACTGTAA CGGAGCACCA TCTCCATCGG 
AGTACAATTG CTGCTCTTCA CTGGTCACCT TTAGTGAAGG ATCTGCTGGT ATCCGGGGAT 
GAAAAAGGAG TATTGGTTTG TTTCTGGTGT AACCGAAATG ACAGCCAGCA GTTTTTTCCA 
GAGCCAAGGA CTATTTTCTG CCTTTCCTGC TCCCCGCACA AGGAGGACAT TGTAGCTATA 
GGGTACAAAG ATGGCATGAT AGTGCTGGTG GATATCAGCC GCAGAGGAGA TGTGTTGCAT 
CGGCTACGAG GGCATGAAGA GGAGATACAC TGTTTGGAAT GGAGCCCCCA TGCCTTGGAG 
TGTGACCTGC AACAAAAGCT GGAGGAGACA GAGATAAACA CAGCCAATGG GGAGCTTCTT 
CCTGAGACAG AAAACAGGAG ATGTTATCTG GCTTCTGGCA GCAAAGACCA GACTGTCCGT 
ATCTGGAGTG CTTCTACAGG CAAAGCAGTT ACAGTGTTGA AGCTGCCATA CTTAAAAAGA 
CGTGGAGGCG GAGTGGATCC AGCCATCAAG GAGCGGCTCT GGTTGACACT GCACTGGCCA 
CCTGGCCATT CAACACAGAT TCTGTCCAGT TGTTTTGGGG GTGAATTGCT CCTGTGGGAT 
TTAACCAAAT CTGGGAAGCA GAAGTGGACT CTCCTTGGCA CCTCTGATGG ACAGAATCAT 
TCTCGAATTG TCTTTAACCT TTGTTCTGTG GTCACTGAGG AAGGGAAAGA GCTCTTGCTG 
TCTACTTCCA TGGACCGAGA TGTGAAATGT TGGGATCTGG GTTCCCTGTC CTGCTGCTGG 
TCCCTAACTT CCCTCGGTGG CTTTGTATAC AGCCTAGCTT TCTCCCCGGT AGCAGCTGGC 
AGCCTGGCTG TTGGGGTGGG GGACAGCATG ATCCGTGTAT GGAATACAAT GTCGGTTGTC 
AATGCATATG ATGTGAAGAC CCTGTGGCAG GGCATCAGAT CCAAGGTCAC TGCGCTGTCA 
TGGCATCCGA CCAAAGAGGG CAGTCTTGCT TTTGGCACCG AGGATGGGAA AGTTGGCATC 
TTTGAGGTCT ACATCAGCAA ACCCCCTCAG ATTTCTGCTA CATACCACAA AAAGACTGTT 
TATTCCTTAT CATGGGGACC TCTGCTGCCT CCATTCTCAT TTGGAGCAGA AGGGGACCGT 
CCTACTTATA CACTGTACAG CTGTGGGGGG GAAGGGGTTA TTTACCAGCA CAATCCTTGG 
AAACTGACAG ATGAAGCCAG TAATATTAAC AAGCTGATCC GGGACACCAA TGACATCAAA 
CACAAGTTAC CAGCACACAC AGAGTTAAGC TGGAGACCTG ATGGACAGTA TTTAGCTATT 
GGCAATGAAG ATGGAACCAT TGAGGTCTTC CACACTCAGC GTCTGCAGCT TATCTGTACC 
ATTCAGCAGC ATCACAAGCT AGTGAATGCT GTGCGCTGGC ATCATGGCCA TAGCCAAGAC 
CCTGACTTGA ACTTTCTGCT GGCATCTGGA TCTAACAATG CTACTGTCTA CGTGCACAAC 
CTGAAAGGGC CCCTGGAGAA TCCCACAGAT TCCCCAGTCA CCATCACTGA GCCTTTCCGT 
ACCTTGCCTG GACACACTGC AAAGATCACA AGCCTGTCAT GGAGCCCTCA CCACGATGCC 
AGACTGGTGT CTGCCAGTTA TGATGGTACT GCCCAGGTAT GGGATGTTCT GCAGGAAGAG 
CCACTCAGTA ACTATCGTGG GCACAAAGGA AGACTGCTTT CTGTTCAGTG GTCTCCTGTG 
GAGCCAGACC AGGTGTGGAC AGGAGCTGAT GACTTTTGCC TTCATAGCTG GTCTGTATCA 
AAGCAAGAGC ATAGCAGGCC TCCCAAAGGG AAAAAAGCCA CTGAGCTTGA AAAAAAGCGT 
ACCCTTCAAC CAAAACCAAA AGCCAAGAAG AAGAAAAAGT TAGACACCCC CAAAGAGGAG 
GCAGGAAGAG AGGTACAGAG GGGAGAAAAG GAAAGCCCTC TAACACAGAA TGGGACATCC 
GATCAAGAGG AGGGACAGGG GACTCCTGAG CCAGAGGCAG CTGACAGAGG CTTACATGAA 
GATGTAGAAT GTCCTTTTCA GCCAGTGGTC CCTTCTCCAG TTCCTGCTCA GGACAAGCAC 
AGTCCCATTT CATTTGCTAA GACTGTACCA GTTAGGAAGG ACCTGGCCAA AGAACGAGCA 
GATCTGCAAA TCAGGAAGAA GAAGAGTCGA TCGTTGCTTC CCATCAGCAC CAGCAAAGAT 
CACCGATCTA AAGAAGAGCA ACACCGAGAC TGTGTTAAGT TGGCAACTGT TGTATGTGCG 
AAGGATTTGG AAGAGAAATC TCATTTTCAC TCGGAGGACA CAATGCACCT GGGACTATAC 
AGTGACCGAG CAGCCCTTTA TCACCTTATG GAGGAGGAGG GTAAGGCTCA TCTGGAGAAT 
GGGCACCCTG AGCTCCAGCA TCAAATTATG CTTTGGAAAG GGGATCTGAA GGGGACTTTG 
CAACTGGCTG CAGAGAGGGG TGAACTGACT GATCAGCTGG TGGCGCTGTC TCCCGTAGCC 
GGATACAAGG TCTGGCTATG GACCGTTGAA GCCTTTGTGA AACAGCTGTG CTTTCATGAG 
CAGTACGTGA AAGCCGCTTC CCACCTGCTG TCCATTAATA AAGTGTATGA AGCCGTGGAA 
CTGCTCAAGG GGAACCACCT CTACAGAGAA GCCATTGCAG TGGCGAAGGC TCGGCTACTC 
CCCGATGATT CAGTCCTGCG GGAGCTGTAC ACTAGCTGGG CAGCAGTCCT TGAGCAGGAC 
GGGCACTATT CCATGGCTGC TAAGTGTTAT TTAGGGTCCT CCTCTCCCTA TGATGCTGCC 
AAGGTTCTAG CTAAGAAGGG GGATTTGCTA TCCCTGAGCG CTGCTGCTGA ACTTGCATTA 
GTTGCAGGAG AAAAAGACCT TGCTGCTTCT TTTGCCATCC GCTGCGCCCA GGAACTGTTC 
ACTGCAAAGA ATTTTTTAGC AGCCCAGGAA GCACTACGAC AGCACAACAG TCTGCTGGGG 
CAGAGACTGC TGTTTTGTAC CAGTGAGCTG CTGTACATGC ATTTGAAGGA GAAGATGCCC 
ATAGATTGGA AGACACAAAG CAATCCATGT TTCCATGAAC AGACAGGCAG TAGTAATGGG 
GAACCTTTTA TGACCAAGCT TACAAGTCTA TGGCAGAGTT TGTGTGAAGT AAACAGCCCA 
GAGCAATACA AAGATGCCTT GCAACAACTG TCTGCCTCAG AGATTCCACA GACCACTGTC 
AATACTGGCC CTAAACAGCT GCTGCTGCAC CTTTCCCATG AGGTCACAAT ATTGTCTCTG 
AATTGTCAGC TTAAAAACTG GAAAGAGGCT GTTCCATCTC TCCTCCATAT CCTGTTCCGC 
TGTCAGCAGG CAGGCCATTT CACCCTGATG CAAGGGATAG CCAGCCTGCT GCTTCCTAAC 
GGCTGTGAAG AGCTGAGGGC TCTGTTGTCC CCAGAAAACA AAGAGAATTT GACTGCCTGT 
GACAGCGTGC AGGCCTTCCT TGCTTACATA AAGATGTATG AGCTGTGGTG GGGACAGCAA 
GAAATGCCAC CAAATTCCAA AGATCCAGCG GCTGCAGTAA ATATGGACAG TCCAAATGGA 
GCCTCTGCTG CTGTGCAGGA GCCAGATATG AAACCAGGGG ATTTGTGCGG CTTTCAGGTG 
TTGCTGTCAG ACCTTCACGC GCCCTTCCAG AGTGCCCAAA GAGAGATTGC TGAGGTTCAG 
AGAGCCCTTC GGGAGATGAT ACAGCAACAC CAGAGAATCA GGCAGAACCA GGACAGCTCT 
TTGGAACTAG AACAAGAGCC TGAATTAAAC ACTTCTTCAT CTCAGGATAA ATCTCTTGAC 
AGCCAAGTCA GCATATCCGC TGGTCCAAGT CCTGTGTCAC TGACAGAGCT GATGAAGAAG 
CTGTCAGTGG CCAACCACCG ACTAGCAGAG TTCCCTGAAT ATTTAAAGGC CTTACCCTTT 
CCTGATGTAC TAGAGAGCTG CCTGGTGCTA CTGCATCTTC CATCCTATCA GATCTCCCCA 
GAGATCCGCT CCCAAGCCTT GGCGTTGTTG CACAGTCATA GCCATGGATC TGCTGATCAC 
CAAATAGCAG CCAAGAAGTT CCTCTCCATC CCAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932422.1
CDS	99..4517
Translation	MGEPDRMLSASPNWYCSRSSDAAGGLFGFAARNSVYLLNINLQEPGFYGELVGHTDRVSGFAFSHFSGQSHLCASSSDDGTVKVWDTQTKNTVTEHHLHRSTIAALHWSPLVKDLLVSGDEKGVLVCFWCNRNDSQQFFPEPRTIFCLSCSPHKEDIVAIGYKDGMIVLVDISRRGDVLHRLRGHEEEIHCLEWSPHALECDLQQKLEETEINTANGELLPETENRRCYLASGSKDQTVRIWSASTGKAVTVLKLPYLKRRGGGVDPAIKERLWLTLHWPPGHSTQILSSCFGGELLLWDLTKSGKQKWTLLGTSDGQNHSRIVFNLCSVVTEEGKELLLSTSMDRDVKCWDLGSLSCCWSLTSLGGFVYSLAFSPVAAGSLAVGVGDSMIRVWNTMSVVNAYDVKTLWQGIRSKVTALSWHPTKEGSLAFGTEDGKVGIFEVYISKPPQISATYHKKTVYSLSWGPLLPPFSFGAEGDRPTYTLYSCGGEGVIYQHNPWKLTDEASNINKLIRDTNDIKHKLPAHTELSWRPDGQYLAIGNEDGTIEVFHTQRLQLICTIQQHHKLVNAVRWHHGHSQDPDLNFLLASGSNNATVYVHNLKGPLENPTDSPVTITEPFRTLPGHTAKITSLSWSPHHDARLVSASYDGTAQVWDVLQEEPLSNYRGHKGRLLSVQWSPVEPDQVWTGADDFCLHSWSVSKQEHSRPPKGKKATELEKKRTLQPKPKAKKKKKLDTPKEEAGREVQRGEKESPLTQNGTSDQEEGQGTPEPEAADRGLHEDVECPFQPVVPSPVPAQDKHSPISFAKTVPVRKDLAKERADLQIRKKKSRSLLPISTSKDHRSKEEQHRDCVKLATVVCAKDLEEKSHFHSEDTMHLGLYSDRAALYHLMEEEGKAHLENGHPELQHQIMLWKGDLKGTLQLAAERGELTDQLVALSPVAGYKVWLWTVEAFVKQLCFHEQYVKAASHLLSINKVYEAVELLKGNHLYREAIAVAKARLLPDDSVLRELYTSWAAVLEQDGHYSMAAKCYLGSSSPYDAAKVLAKKGDLLSLSAAAELALVAGEKDLAASFAIRCAQELFTAKNFLAAQEALRQHNSLLGQRLLFCTSELLYMHLKEKMPIDWKTQSNPCFHEQTGSSNGEPFMTKLTSLWQSLCEVNSPEQYKDALQQLSASEIPQTTVNTGPKQLLLHLSHEVTILSLNCQLKNWKEAVPSLLHILFRCQQAGHFTLMQGIASLLLPNGCEELRALLSPENKENLTACDSVQAFLAYIKMYELWWGQQEMPPNSKDPAAAVNMDSPNGASAAVQEPDMKPGDLCGFQVLLSDLHAPFQSAQREIAEVQRALREMIQQHQRIRQNQDSSLELEQEPELNTSSSQDKSLDSQVSISAGPSPVSLTELMKKLSVANHRLAEFPEYLKALPFPDVLESCLVLLHLPSYQISPEIRSQALALLHSHSHGSADHQIAAKKFLSIPE

Target ORF information:

RefSeq Version	XM_002932376.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gem nuclear organelle associated protein 5 (gemin5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932376.2

ORF Insert Sequence:

ATGGGGGAAC CAGATCGGAT GCTCAGTGCG TCACCCAACT GGTACTGTAG CCGCTCCAGT 
GATGCTGCCG GTGGACTGTT CGGATTCGCT GCTCGAAATT CTGTCTACCT CCTGAACATT 
AATCTGCAGG AGCCTGGATT TTATGGAGAG CTGGTTGGAC ATACAGACCG CGTCTCTGGC 
TTTGCTTTTT CCCATTTCTC AGGCCAATCT CACCTGTGTG CCAGTAGCTC TGATGATGGT 
ACTGTAAAGG TTTGGGACAC GCAGACAAAG AACACTGTAA CGGAGCACCA TCTCCATCGG 
AGTACAATTG CTGCTCTTCA CTGGTCACCT TTAGTGAAGG ATCTGCTGGT ATCCGGGGAT 
GAAAAAGGAG TATTGGTTTG TTTCTGGTGT AACCGAAATG ACAGCCAGCA GTTTTTTCCA 
GAGCCAAGGA CTATTTTCTG CCTTTCCTGC TCCCCGCACA AGGAGGACAT TGTAGCTATA 
GGGTACAAAG ATGGCATGAT AGTGCTGGTG GATATCAGCC GCAGAGGAGA TGTGTTGCAT 
CGGCTACGAG GGCATGAAGA GGAGATACAC TGTTTGGAAT GGAGCCCCCA TGCCTTGGAG 
TGTGACCTGC AACAAAAGCT GGAGGAGACA GAGATAAACA CAGCCAATGG GGAGCTTCTT 
CCTGAGACAG AAAACAGGAG ATGTTATCTG GCTTCTGGCA GCAAAGACCA GACTGTCCGT 
ATCTGGAGTG CTTCTACAGG CAAAGCAGTT ACAGTGTTGA AGCTGCCATA CTTAAAAAGA 
CGTGGAGGCG GAGTGGATCC AGCCATCAAG GAGCGGCTCT GGTTGACACT GCACTGGCCA 
CCTGGCCATT CAACACAGAT TCTGTCCAGT TGTTTTGGGG GTGAATTGCT CCTGTGGGAT 
TTAACCAAAT CTGGGAAGCA GAAGTGGACT CTCCTTGGCA CCTCTGATGG ACAGAATCAT 
TCTCGAATTG TCTTTAACCT TTGTTCTGTG GTCACTGAGG AAGGGAAAGA GCTCTTGCTG 
TCTACTTCCA TGGACCGAGA TGTGAAATGT TGGGATCTGG GTTCCCTGTC CTGCTGCTGG 
TCCCTAACTT CCCTCGGTGG CTTTGTATAC AGCCTAGCTT TCTCCCCGGT AGCAGCTGGC 
AGCCTGGCTG TTGGGGTGGG GGACAGCATG ATCCGTGTAT GGAATACAAT GTCGGTTGTC 
AATGCATATG ATGTGAAGAC CCTGTGGCAG GGCATCAGAT CCAAGGTCAC TGCGCTGTCA 
TGGCATCCGA CCAAAGAGGG CAGTCTTGCT TTTGGCACCG AGGATGGGAA AGTTGGCATC 
TTTGAGGTCT ACATCAGCAA ACCCCCTCAG ATTTCTGCTA CATACCACAA AAAGACTGTT 
TATTCCTTAT CATGGGGACC TCTGCTGCCT CCATTCTCAT TTGGAGCAGA AGGGGACCGT 
CCTACTTATA CACTGTACAG CTGTGGGGGG GAAGGGGTTA TTTACCAGCA CAATCCTTGG 
AAACTGACAG ATGAAGCCAG TAATATTAAC AAGCTGATCC GGGACACCAA TGACATCAAA 
CACAAGTTAC CAGCACACAC AGAGTTAAGC TGGAGACCTG ATGGACAGTA TTTAGCTATT 
GGCAATGAAG ATGGAACCAT TGAGGTCTTC CACACTCAGC GTCTGCAGCT TATCTGTACC 
ATTCAGCAGC ATCACAAGCT AGTGAATGCT GTGCGCTGGC ATCATGGCCA TAGCCAAGAC 
CCTGACTTGA ACTTTCTGCT GGCATCTGGA TCTAACAATG CTACTGTCTA CGTGCACAAC 
CTGAAAGGGC CCCTGGAGAA TCCCACAGAT TCCCCAGTCA CCATCACTGA GCCTTTCCGT 
ACCTTGCCTG GACACACTGC AAAGATCACA AGCCTGTCAT GGAGCCCTCA CCACGATGCC 
AGACTGGTGT CTGCCAGTTA TGATGGTACT GCCCAGGTAT GGGATGTTCT GCAGGAAGAG 
CCACTCAGTA ACTATCGTGG GCACAAAGGA AGACTGCTTT CTGTTCAGTG GTCTCCTGTG 
GAGCCAGACC AGGTGTGGAC AGGAGCTGAT GACTTTTGCC TTCATAGCTG GTCTGTATCA 
AAGCAAGAGC ATAGCAGGCC TCCCAAAGGG AAAAAAGCCA CTGAGCTTGA AAAAAAGCGT 
ACCCTTCAAC CAAAACCAAA AGCCAAGAAG AAGAAAAAGT TAGACACCCC CAAAGAGGAG 
GCAGGAAGAG AGGTACAGAG GGGAGAAAAG GAAAGCCCTC TAACACAGAA TGGGACATCC 
GATCAAGAGG AGGGACAGGG GACTCCTGAG CCAGAGGCAG CTGACAGAGG CTTACATGAA 
GATGTAGAAT GTCCTTTTCA GCCAGTGGTC CCTTCTCCAG TTCCTGCTCA GGACAAGCAC 
AGTCCCATTT CATTTGCTAA GACTGTACCA GTTAGGAAGG ACCTGGCCAA AGAACGAGCA 
GATCTGCAAA TCAGGAAGAA GAAGAGTCGA TCGTTGCTTC CCATCAGCAC CAGCAAAGAT 
CACCGATCTA AAGAAGAGCA ACACCGAGAC TGTGTTAAGT TGGCAACTGT TGTATGTGCG 
AAGGATTTGG AAGAGAAATC TCATTTTCAC TCGGAGGACA CAATGCACCT GGGACTATAC 
AGTGACCGAG CAGCCCTTTA TCACCTTATG GAGGAGGAGG GTAAGGCTCA TCTGGAGAAT 
GGGCACCCTG AGCTCCAGCA TCAAATTATG CTTTGGAAAG GGGATCTGAA GGGGACTTTG 
CAACTGGCTG CAGAGAGGGG TGAACTGACT GATCAGCTGG TGGCGCTGTC TCCCGTAGCC 
GGATACAAGG TCTGGCTATG GACCGTTGAA GCCTTTGTGA AACAGCTGTG CTTTCATGAG 
CAGTACGTGA AAGCCGCTTC CCACCTGCTG TCCATTAATA AAGTGTATGA AGCCGTGGAA 
CTGCTCAAGG GGAACCACCT CTACAGAGAA GCCATTGCAG TGGCGAAGGC TCGGCTACTC 
CCCGATGATT CAGTCCTGCG GGAGCTGTAC ACTAGCTGGG CAGCAGTCCT TGAGCAGGAC 
GGGCACTATT CCATGGCTGC TAAGTGTTAT TTAGGGTCCT CCTCTCCCTA TGATGCTGCC 
AAGGTTCTAG CTAAGAAGGG GGATTTGCTA TCCCTGAGCG CTGCTGCTGA ACTTGCATTA 
GTTGCAGGAG AAAAAGACCT TGCTGCTTCT TTTGCCATCC GCTGCGCCCA GGAACTGTTC 
ACTGCAAAGA ATTTTTTAGC AGCCCAGGAA GCACTACGAC AGCACAACAG TCTGCTGGGG 
CAGAGACTGC TGTTTTGTAC CAGTGAGCTG CTGTACATGC ATTTGAAGGA GAAGATGCCC 
ATAGATTGGA AGACACAAAG CAATCCATGT TTCCATGAAC AGACAGGCAG TAGTAATGGG 
GAACCTTTTA TGACCAAGCT TACAAGTCTA TGGCAGAGTT TGTGTGAAGT AAACAGCCCA 
GAGCAATACA AAGATGCCTT GCAACAACTG TCTGCCTCAG AGATTCCACA GACCACTGTC 
AATACTGGCC CTAAACAGCT GCTGCTGCAC CTTTCCCATG AGGTCACAAT ATTGTCTCTG 
AATTGTCAGC TTAAAAACTG GAAAGAGGCT GTTCCATCTC TCCTCCATAT CCTGTTCCGC 
TGTCAGCAGG CAGGCCATTT CACCCTGATG CAAGGGATAG CCAGCCTGCT GCTTCCTAAC 
GGCTGTGAAG AGCTGAGGGC TCTGTTGTCC CCAGAAAACA AAGAGAATTT GACTGCCTGT 
GACAGCGTGC AGGCCTTCCT TGCTTACATA AAGATGTATG AGCTGTGGTG GGGACAGCAA 
GAAATGCCAC CAAATTCCAA AGATCCAGCG GCTGCAGTAA ATATGGACAG TCCAAATGGA 
GCCTCTGCTG CTGTGCAGGA GCCAGATATG AAACCAGGGG ATTTGTGCGG CTTTCAGGTG 
TTGCTGTCAG ACCTTCACGC GCCCTTCCAG AGTGCCCAAA GAGAGATTGC TGAGGTTCAG 
AGAGCCCTTC GGGAGATGAT ACAGCAACAC CAGAGAATCA GGCAGAACCA GGACAGCTCT 
TTGGAACTAG AACAAGAGCC TGAATTAAAC ACTTCTTCAT CTCAGGATAA ATCTCTTGAC 
AGCCAAGTCA GCATATCCGC TGGTCCAAGT CCTGTGTCAC TGACAGAGCT GATGAAGAAG 
CTGTCAGTGG CCAACCACCG ACTAGCAGAG TTCCCTGAAT ATTTAAAGGC CTTACCCTTT 
CCTGATGTAC TAGAGAGCTG CCTGGTGCTA CTGCATCTTC CATCCTATCA GATCTCCCCA 
GAGATCCGCT CCCAAGCCTT GGCGTTGTTG CACAGTCATA GCCATGGATC TGCTGATCAC 
CAAATAGCAG CCAAGAAGTT CCTCTCCATC CCAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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gemin5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

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FAX

オンラインフォーム

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