TMIE cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMIE
Entrez Gene ID	259236
Full Name	transmembrane inner ear
Synonyms	DFNB6
General protein information	Preferred Names transmembrane inner ear Names transmembrane inner ear expressed protein transmembrane inner ear protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.31
Summary	This gene encodes a transmembrane inner ear protein. Studies in mouse suggest that this gene is required for normal postnatal maturation of sensory hair cells in the cochlea, including correct development of stereocilia bundles. This gene is one of multiple genes responsible for recessive non-syndromic deafness (DFNB), also known as autosomal recessive nonsyndromic hearing loss (ARNSHL), the most common form of congenitally acquired inherited hearing impairment. [provided by RefSeq, Mar 2009].
Disorder MIM:	607237
Disorder Html:	Deafness, autosomal recessive 6, 600971 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006713097.4	XP_006713160.1	transmembrane inner ear expressed protein isoform X1
NM_001370524.1	NP_001357453.1	transmembrane inner ear expressed protein isoform 2
NM_001370525.1	NP_001357454.1	transmembrane inner ear expressed protein isoform 2
XM_006713097.3	XP_006713160.1	transmembrane inner ear expressed protein isoform X1
NM_147196.2	NP_671729.2	transmembrane inner ear expressed protein isoform 1 precursor
XM_024453446.1	XP_024309214.1	transmembrane inner ear expressed protein isoform X1
NM_147196.3	NP_671729.2	transmembrane inner ear expressed protein isoform 1 precursor

Pathways from BioSystems

Homologies

	LOC712706	XP_001113938.1
Canis lupus familiaris (dog)	TMIE	XP_851689.2
Mus musculus (house mouse)	Tmie	NP_666372.1
Rattus norvegicus (Norway rat)	Tmie	NP_001102769.1
Pan troglodytes (chimpanzee)	TMIE	XP_001151741.1
Homo sapiens (human)	TMIE	NP_671729.2
Bos taurus (cattle)	TMIE	NP_001193663.1

Gene Ontology
Bibliography

Related articles in PubMed

Non-syndromic hearing impairment in India: high allelic heterogeneity among mutations in TMPRSS3, TMC1, USHIC, CDH23 and TMIE.
Ganapathy A, Pandey N, Srisailapathy CR, Jalvi R, Malhotra V, Venkatappa M, Chatterjee A, Sharma M, Santhanam R, Chadha S, Ramesh A, Agarwal AK, Rangasayee RR, Anand A
PloS one9(1)e84773(2014)

Identification of novel variants in the TMIE gene of patients with nonsyndromic hearing loss.
Yang JJ, Su MC, Chien KH, Hsin CH, Li SY
International journal of pediatric otorhinolaryngology74(5)489-93(2010 May)

A founder TMIE mutation is a frequent cause of hearing loss in southeastern Anatolia.
Sirmaci A, Ozt?rkmen-Akay H, Erbek S, Incesulu A, Duman D, Ta?ir-Yilmaz S, Ozda? H, Tekin M
Clinical genetics75(6)562-7(2009 Jun)

Novel sequence variants in the TMIE gene in families with autosomal recessive nonsyndromic hearing impairment.
Santos RL, El-Shanti H, Sikandar S, Lee K, Bhatti A, Yan K, Chahrour MH, McArthur N, Pham TL, Mahasneh AA, Ahmad W, Leal SM
Journal of molecular medicine (Berlin, Germany)84(3)226-31(2006 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Description of the spectrum of mutations in TMIE in 374 families with autosomal recessive, non-syndromic hearing loss from India.
Title: Non-syndromic hearing impairment in India: high allelic heterogeneity among mutations in TMPRSS3, TMC1, USHIC, CDH23 and TMIE.

Observational study of gene-disease association. (HuGE Navigator)
Title: Identification of novel variants in the TMIE gene of patients with nonsyndromic hearing loss.

p.R84W could be a common mutation in other Middle Eastern populations and should be included in mutation screening offered to individuals with autosomal recessive non-syndromic sensorineural hearing loss (ARNSSNHL)
Title: A founder TMIE mutation is a frequent cause of hearing loss in southeastern Anatolia.

The circling mouse is a potential animal model for DFNB6 deafness in humans.
Title: Fine mapping of the circling (cir) gene on the distal portion of mouse chromosome 9.

identification of 5 different homozygous recessive mutations in a novel gene, TMIE= transmembrane inner ear expressed gene, in affected members of consanguineous families segregating severe-to-profound prelingual deafness, consistent with linkage to DFNB6
Title: Mutations in a novel gene, TMIE, are associated with hearing loss linked to the DFNB6 locus.

The following TMIE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMIE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21318	NM_147196.3 Latest version!	Homo sapiens transmembrane inner ear (TMIE), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu37152	NM_001370524.1 Latest version!	Homo sapiens transmembrane inner ear (TMIE), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
OHu37152	NM_001370525.1 Latest version!	Homo sapiens transmembrane inner ear (TMIE), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
OHu37152	XM_024453446.1 Latest version!	Homo sapiens transmembrane inner ear (TMIE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
OHu37152	XM_006713097.4 Latest version!	Homo sapiens transmembrane inner ear (TMIE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
OHu37152	XM_006713097.3	Homo sapiens transmembrane inner ear (TMIE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
OHu21318	NM_147196.2	Homo sapiens transmembrane inner ear (TMIE), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMIE ( NM_147196.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_671729.2 Homo sapiens transmembrane inner ear (TMIE), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21318
Clone ID Related Accession (Same CDS sequence)	NM_147196.2 , NM_147196.3
Accession Version	NM_147196.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-08
Organism	Homo sapiens(human)
Product	transmembrane inner ear expressed protein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY081842.1 and AC134504.2. This sequence is a reference standard in the RefSeqGene project. On Oct 24, 2007 this sequence version replaced NM_147196.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY081842.1, SRR1803617.92397.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT GGCCGGGCGC GGGTCCCCTC TGCGTGCTGG GCGGCGCCGC ACTCGGGGTG 
TGCCTCGCGG GGGTTGCCGG GCAGCTGGTG GAGCCCAGCA CGGCCCCACC CAAGCCCAAG 
CCGCCTCCGC TGACCAAGGA GACAGTGGTG TTCTGGGACA TGCGCCTGTG GCACGTGGTG 
GGCATCTTTT CGCTCTTCGT GTTGTCCATC ATCATCACGC TGTGCTGTGT CTTCAACTGT 
CGTGTGCCAC GGACCCGGAA GGAGATCGAA GCCCGGTACC TGCAGCGAAA GGCAGCCAAG 
ATGTACACAG ACAAGCTGGA GACTGTGCCA CCCCTCAATG AGCTCACAGA AGTCCCAGGA 
GAGGATAAGA AGAAGAAGAA GAAGAAGAAG AAGGACAGTG TGGACACAGT GGCCATCAAA 
GTAGAGGAGG ATGAGAAGAA TGAGGCCAAG AAGAAGAAAG GAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_671729.2
CDS	156..626
Translation	MAGWPGAGPLCVLGGAALGVCLAGVAGQLVEPSTAPPKPKPPPLTKETVVFWDMRLWHVVGIFSLFVLSIIITLCCVFNCRVPRTRKEIEARYLQRKAAKMYTDKLETVPPLNELTEVPGEDKKKKKKKKKDSVDTVAIKVEEDEKNEAKKKKGEK

Target ORF information:

RefSeq Version	NM_147196.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane inner ear (TMIE), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_147196.2

ORF Insert Sequence:

ATGGCGGGGT GGCCGGGCGC GGGTCCCCTC TGCGTGCTGG GCGGCGCCGC ACTCGGGGTG 
TGCCTCGCGG GGGTTGCCGG GCAGCTGGTG GAGCCCAGCA CGGCCCCACC CAAGCCCAAG 
CCGCCTCCGC TGACCAAGGA GACAGTGGTG TTCTGGGACA TGCGCCTGTG GCACGTGGTG 
GGCATCTTTT CGCTCTTCGT GTTGTCCATC ATCATCACGC TGTGCTGTGT CTTCAACTGT 
CGTGTGCCAC GGACCCGGAA GGAGATCGAA GCCCGGTACC TGCAGCGAAA GGCAGCCAAG 
ATGTACACAG ACAAGCTGGA GACTGTGCCA CCCCTCAATG AGCTCACAGA AGTCCCAGGA 
GAGGATAAGA AGAAGAAGAA GAAGAAGAAG AAGGACAGTG TGGACACAGT GGCCATCAAA 
GTAGAGGAGG ATGAGAAGAA TGAGGCCAAG AAGAAGAAAG GAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMIE ( NM_147196.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_671729.2 Homo sapiens transmembrane inner ear (TMIE), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21318
Clone ID Related Accession (Same CDS sequence)	NM_147196.2 , NM_147196.3
Accession Version	NM_147196.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	transmembrane inner ear expressed protein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC134504.2. On Sep 24, 2019 this sequence version replaced NM_147196.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY081842.1, BC126260.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGGGT GGCCGGGCGC GGGTCCCCTC TGCGTGCTGG GCGGCGCCGC ACTCGGGGTG 
TGCCTCGCGG GGGTTGCCGG GCAGCTGGTG GAGCCCAGCA CGGCCCCACC CAAGCCCAAG 
CCGCCTCCGC TGACCAAGGA GACAGTGGTG TTCTGGGACA TGCGCCTGTG GCACGTGGTG 
GGCATCTTTT CGCTCTTCGT GTTGTCCATC ATCATCACGC TGTGCTGTGT CTTCAACTGT 
CGTGTGCCAC GGACCCGGAA GGAGATCGAA GCCCGGTACC TGCAGCGAAA GGCAGCCAAG 
ATGTACACAG ACAAGCTGGA GACTGTGCCA CCCCTCAATG AGCTCACAGA AGTCCCAGGA 
GAGGATAAGA AGAAGAAGAA GAAGAAGAAG AAGGACAGTG TGGACACAGT GGCCATCAAA 
GTAGAGGAGG ATGAGAAGAA TGAGGCCAAG AAGAAGAAAG GAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_671729.2
CDS	97..567
Translation	MAGWPGAGPLCVLGGAALGVCLAGVAGQLVEPSTAPPKPKPPPLTKETVVFWDMRLWHVVGIFSLFVLSIIITLCCVFNCRVPRTRKEIEARYLQRKAAKMYTDKLETVPPLNELTEVPGEDKKKKKKKKKDSVDTVAIKVEEDEKNEAKKKKGEK

Target ORF information:

RefSeq Version	NM_147196.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane inner ear (TMIE), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_147196.3

ORF Insert Sequence:

ATGGCGGGGT GGCCGGGCGC GGGTCCCCTC TGCGTGCTGG GCGGCGCCGC ACTCGGGGTG 
TGCCTCGCGG GGGTTGCCGG GCAGCTGGTG GAGCCCAGCA CGGCCCCACC CAAGCCCAAG 
CCGCCTCCGC TGACCAAGGA GACAGTGGTG TTCTGGGACA TGCGCCTGTG GCACGTGGTG 
GGCATCTTTT CGCTCTTCGT GTTGTCCATC ATCATCACGC TGTGCTGTGT CTTCAACTGT 
CGTGTGCCAC GGACCCGGAA GGAGATCGAA GCCCGGTACC TGCAGCGAAA GGCAGCCAAG 
ATGTACACAG ACAAGCTGGA GACTGTGCCA CCCCTCAATG AGCTCACAGA AGTCCCAGGA 
GAGGATAAGA AGAAGAAGAA GAAGAAGAAG AAGGACAGTG TGGACACAGT GGCCATCAAA 
GTAGAGGAGG ATGAGAAGAA TGAGGCCAAG AAGAAGAAAG GAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMIE ( NM_001370524.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357453.1 Homo sapiens transmembrane inner ear (TMIE), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu37152
Clone ID Related Accession (Same CDS sequence)	XM_006713097.4 , NM_001370524.1 , NM_001370525.1 , XM_006713097.3 , XM_024453446.1
Accession Version	NM_001370524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	transmembrane inner ear expressed protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC134504.2. On May 9, 2019 this sequence version replaced XM_006713097.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.222480.1, SRR1803615.432034.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCGCCTGT GGCACGTGGT GGGCATCTTT TCGCTCTTCG TGTTGTCCAT CATCATCACG 
CTGTGCTGTG TCTTCAACTG TCGTGTGCCA CGGACCCGGA AGGAGATCGA AGCCCGGTAC 
CTGCAGCGAA AGGCAGCCAA GATGTACACA GACAAGCTGG AGACTGTGCC ACCCCTCAAT 
GAGCTCACAG AAGTCCCAGG AGAGGATAAG AAGAAGAAGA AGAAGAAGAA GAAGGACAGT 
GTGGACACAG TGGCCATCAA AGTAGAGGAG GATGAGAAGA ATGAGGCCAA GAAGAAGAAA 
GGAGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357453.1
CDS	908..1219
Translation	MRLWHVVGIFSLFVLSIIITLCCVFNCRVPRTRKEIEARYLQRKAAKMYTDKLETVPPLNELTEVPGEDKKKKKKKKKDSVDTVAIKVEEDEKNEAKKKKGEK

Target ORF information:

RefSeq Version	NM_001370524.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane inner ear (TMIE), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370524.1

ORF Insert Sequence:

ATGCGCCTGT GGCACGTGGT GGGCATCTTT TCGCTCTTCG TGTTGTCCAT CATCATCACG 
CTGTGCTGTG TCTTCAACTG TCGTGTGCCA CGGACCCGGA AGGAGATCGA AGCCCGGTAC 
CTGCAGCGAA AGGCAGCCAA GATGTACACA GACAAGCTGG AGACTGTGCC ACCCCTCAAT 
GAGCTCACAG AAGTCCCAGG AGAGGATAAG AAGAAGAAGA AGAAGAAGAA GAAGGACAGT 
GTGGACACAG TGGCCATCAA AGTAGAGGAG GATGAGAAGA ATGAGGCCAA GAAGAAGAAA 
GGAGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMIE ( NM_001370525.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357454.1 Homo sapiens transmembrane inner ear (TMIE), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu37152
Clone ID Related Accession (Same CDS sequence)	XM_006713097.4 , NM_001370524.1 , NM_001370525.1 , XM_006713097.3 , XM_024453446.1
Accession Version	NM_001370525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	transmembrane inner ear expressed protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC134504.2. On May 9, 2019 this sequence version replaced XM_024453446.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.871365.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCGCCTGT GGCACGTGGT GGGCATCTTT TCGCTCTTCG TGTTGTCCAT CATCATCACG 
CTGTGCTGTG TCTTCAACTG TCGTGTGCCA CGGACCCGGA AGGAGATCGA AGCCCGGTAC 
CTGCAGCGAA AGGCAGCCAA GATGTACACA GACAAGCTGG AGACTGTGCC ACCCCTCAAT 
GAGCTCACAG AAGTCCCAGG AGAGGATAAG AAGAAGAAGA AGAAGAAGAA GAAGGACAGT 
GTGGACACAG TGGCCATCAA AGTAGAGGAG GATGAGAAGA ATGAGGCCAA GAAGAAGAAA 
GGAGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357454.1
CDS	598..909
Translation	MRLWHVVGIFSLFVLSIIITLCCVFNCRVPRTRKEIEARYLQRKAAKMYTDKLETVPPLNELTEVPGEDKKKKKKKKKDSVDTVAIKVEEDEKNEAKKKKGEK

Target ORF information:

RefSeq Version	NM_001370525.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane inner ear (TMIE), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370525.1

ORF Insert Sequence:

ATGCGCCTGT GGCACGTGGT GGGCATCTTT TCGCTCTTCG TGTTGTCCAT CATCATCACG 
CTGTGCTGTG TCTTCAACTG TCGTGTGCCA CGGACCCGGA AGGAGATCGA AGCCCGGTAC 
CTGCAGCGAA AGGCAGCCAA GATGTACACA GACAAGCTGG AGACTGTGCC ACCCCTCAAT 
GAGCTCACAG AAGTCCCAGG AGAGGATAAG AAGAAGAAGA AGAAGAAGAA GAAGGACAGT 
GTGGACACAG TGGCCATCAA AGTAGAGGAG GATGAGAAGA ATGAGGCCAA GAAGAAGAAA 
GGAGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMIE ( XM_006713097.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713160.1 Homo sapiens transmembrane inner ear (TMIE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu37152
Clone ID Related Accession (Same CDS sequence)	XM_006713097.4 , NM_001370524.1 , NM_001370525.1 , XM_006713097.3 , XM_024453446.1
Accession Version	XM_006713097.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	transmembrane inner ear expressed protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006713097.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCTGT GGCACGTGGT GGGCATCTTT TCGCTCTTCG TGTTGTCCAT CATCATCACG 
CTGTGCTGTG TCTTCAACTG TCGTGTGCCA CGGACCCGGA AGGAGATCGA AGCCCGGTAC 
CTGCAGCGAA AGGCAGCCAA GATGTACACA GACAAGCTGG AGACTGTGCC ACCCCTCAAT 
GAGCTCACAG AAGTCCCAGG AGAGGATAAG AAGAAGAAGA AGAAGAAGAA GAAGGACAGT 
GTGGACACAG TGGCCATCAA AGTAGAGGAG GATGAGAAGA ATGAGGCCAA GAAGAAGAAA 
GGAGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713160.1
CDS	908..1219
Translation	MRLWHVVGIFSLFVLSIIITLCCVFNCRVPRTRKEIEARYLQRKAAKMYTDKLETVPPLNELTEVPGEDKKKKKKKKKDSVDTVAIKVEEDEKNEAKKKKGEK

Target ORF information:

RefSeq Version	XM_006713097.4
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane inner ear (TMIE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713097.4

ORF Insert Sequence:

ATGCGCCTGT GGCACGTGGT GGGCATCTTT TCGCTCTTCG TGTTGTCCAT CATCATCACG 
CTGTGCTGTG TCTTCAACTG TCGTGTGCCA CGGACCCGGA AGGAGATCGA AGCCCGGTAC 
CTGCAGCGAA AGGCAGCCAA GATGTACACA GACAAGCTGG AGACTGTGCC ACCCCTCAAT 
GAGCTCACAG AAGTCCCAGG AGAGGATAAG AAGAAGAAGA AGAAGAAGAA GAAGGACAGT 
GTGGACACAG TGGCCATCAA AGTAGAGGAG GATGAGAAGA ATGAGGCCAA GAAGAAGAAA 
GGAGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMIE ( XM_006713097.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713160.1 Homo sapiens transmembrane inner ear (TMIE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu37152
Clone ID Related Accession (Same CDS sequence)	XM_006713097.4 , NM_001370524.1 , NM_001370525.1 , XM_006713097.3 , XM_024453446.1
Accession Version	XM_006713097.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	transmembrane inner ear expressed protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713097.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCTGT GGCACGTGGT GGGCATCTTT TCGCTCTTCG TGTTGTCCAT CATCATCACG 
CTGTGCTGTG TCTTCAACTG TCGTGTGCCA CGGACCCGGA AGGAGATCGA AGCCCGGTAC 
CTGCAGCGAA AGGCAGCCAA GATGTACACA GACAAGCTGG AGACTGTGCC ACCCCTCAAT 
GAGCTCACAG AAGTCCCAGG AGAGGATAAG AAGAAGAAGA AGAAGAAGAA GAAGGACAGT 
GTGGACACAG TGGCCATCAA AGTAGAGGAG GATGAGAAGA ATGAGGCCAA GAAGAAGAAA 
GGAGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713160.1
CDS	886..1197
Translation	MRLWHVVGIFSLFVLSIIITLCCVFNCRVPRTRKEIEARYLQRKAAKMYTDKLETVPPLNELTEVPGEDKKKKKKKKKDSVDTVAIKVEEDEKNEAKKKKGEK

Target ORF information:

RefSeq Version	XM_006713097.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane inner ear (TMIE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713097.3

ORF Insert Sequence:

ATGCGCCTGT GGCACGTGGT GGGCATCTTT TCGCTCTTCG TGTTGTCCAT CATCATCACG 
CTGTGCTGTG TCTTCAACTG TCGTGTGCCA CGGACCCGGA AGGAGATCGA AGCCCGGTAC 
CTGCAGCGAA AGGCAGCCAA GATGTACACA GACAAGCTGG AGACTGTGCC ACCCCTCAAT 
GAGCTCACAG AAGTCCCAGG AGAGGATAAG AAGAAGAAGA AGAAGAAGAA GAAGGACAGT 
GTGGACACAG TGGCCATCAA AGTAGAGGAG GATGAGAAGA ATGAGGCCAA GAAGAAGAAA 
GGAGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMIE ( XM_024453446.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309214.1 Homo sapiens transmembrane inner ear (TMIE), transcript variant X2, mRNA.

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CloneID	OHu37152
Clone ID Related Accession (Same CDS sequence)	XM_006713097.4 , NM_001370524.1 , NM_001370525.1 , XM_006713097.3 , XM_024453446.1
Accession Version	XM_024453446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	transmembrane inner ear expressed protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCTGT GGCACGTGGT GGGCATCTTT TCGCTCTTCG TGTTGTCCAT CATCATCACG 
CTGTGCTGTG TCTTCAACTG TCGTGTGCCA CGGACCCGGA AGGAGATCGA AGCCCGGTAC 
CTGCAGCGAA AGGCAGCCAA GATGTACACA GACAAGCTGG AGACTGTGCC ACCCCTCAAT 
GAGCTCACAG AAGTCCCAGG AGAGGATAAG AAGAAGAAGA AGAAGAAGAA GAAGGACAGT 
GTGGACACAG TGGCCATCAA AGTAGAGGAG GATGAGAAGA ATGAGGCCAA GAAGAAGAAA 
GGAGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309214.1
CDS	576..887
Translation	MRLWHVVGIFSLFVLSIIITLCCVFNCRVPRTRKEIEARYLQRKAAKMYTDKLETVPPLNELTEVPGEDKKKKKKKKKDSVDTVAIKVEEDEKNEAKKKKGEK

Target ORF information:

RefSeq Version	XM_024453446.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane inner ear (TMIE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453446.1

ORF Insert Sequence:

ATGCGCCTGT GGCACGTGGT GGGCATCTTT TCGCTCTTCG TGTTGTCCAT CATCATCACG 
CTGTGCTGTG TCTTCAACTG TCGTGTGCCA CGGACCCGGA AGGAGATCGA AGCCCGGTAC 
CTGCAGCGAA AGGCAGCCAA GATGTACACA GACAAGCTGG AGACTGTGCC ACCCCTCAAT 
GAGCTCACAG AAGTCCCAGG AGAGGATAAG AAGAAGAAGA AGAAGAAGAA GAAGGACAGT 
GTGGACACAG TGGCCATCAA AGTAGAGGAG GATGAGAAGA ATGAGGCCAA GAAGAAGAAA 
GGAGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Non-syndromic hearing impairment in India: high allelic heterogeneity among mutations in TMPRSS3, TMC1, USHIC, CDH23 and TMIE.
PloS one9(1)e84773(2014)
Ganapathy A,Pandey N,Srisailapathy CR,Jalvi R,Malhotra V,Venkatappa M,Chatterjee A,Sharma M,Santhanam R,Chadha S,Ramesh A,Agarwal AK,Rangasayee RR,Anand A

Identification of novel variants in the TMIE gene of patients with nonsyndromic hearing loss.
International journal of pediatric otorhinolaryngology74(5)489-93(2010 May)
Yang JJ,Su MC,Chien KH,Hsin CH,Li SY

A founder TMIE mutation is a frequent cause of hearing loss in southeastern Anatolia.
Clinical genetics75(6)562-7(2009 Jun)
Sirmaci A,Ozt?rkmen-Akay H,Erbek S,Incesulu A,Duman D,Ta?ir-Yilmaz S,Ozda? H,Tekin M

Novel sequence variants in the TMIE gene in families with autosomal recessive nonsyndromic hearing impairment.
Journal of molecular medicine (Berlin, Germany)84(3)226-31(2006 Mar)
Santos RL,El-Shanti H,Sikandar S,Lee K,Bhatti A,Yan K,Chahrour MH,McArthur N,Pham TL,Mahasneh AA,Ahmad W,Leal SM

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TMIE cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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