PRKD2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKD2
Entrez Gene ID	25865
Full Name	protein kinase D2
Synonyms	HSPC187,PKD2,nPKC-D2
General protein information	Preferred Names protein kinase D2 Names serine/threonine-protein kinase D2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.32
Summary	The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].
Disorder MIM:	607074

mRNA and Protein(s)

mRNA	Protein	Name
NM_016457.4	NP_057541.2	serine/threonine-protein kinase D2 isoform A
NM_001079882.1	NP_001073351.1	serine/threonine-protein kinase D2 isoform B
XM_005258716.2	XP_005258773.2	serine/threonine-protein kinase D2 isoform X1
NM_001079881.1	NP_001073350.1	serine/threonine-protein kinase D2 isoform A
NM_001079880.1	NP_001073349.1	serine/threonine-protein kinase D2 isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRKD2	NP_057541.2
Pan troglodytes (chimpanzee)	PRKD2	XP_524314.3
Rattus norvegicus (Norway rat)	Prkd2	NP_001013917.1
Mus musculus (house mouse)	Prkd2	NP_001239387.1
Bos taurus (cattle)	PRKD2	XP_005219507.1
Canis lupus familiaris (dog)	PRKD2	XP_005616468.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein kinase D2: a versatile player in cancer biology.
Azoitei N, Cobbaut M, Becher A, Van Lint J, Seufferlein T
Oncogene(2017 Dec)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Differential regulation of PKD isoforms in oxidative stress conditions through phosphorylation of a conserved Tyr in the P+1 loop.
Cobbaut M, Derua R, D?ppler H, Lou HJ, Vandoninck S, Storz P, Turk BE, Seufferlein T, Waelkens E, Janssens V, Van Lint J
Scientific reports7(1)887(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study discovered and characterized a novel, highly conserved N-terminal domain, comprising 92 amino acids, which mediates dimerization of Protein Kinase D (PKD) isoforms, PKD1, PKD2, and PKD3 monomers.
Title: A Novel Conserved Domain Mediates Dimerization of Protein Kinase D (PKD) Isoforms: DIMERIZATION IS ESSENTIAL FOR PKD-DEPENDENT REGULATION OF SECRETION AND INNATE IMMUNITY.

None of the Polymorphous low-grade adenocarcinoma (PLGA) lacking PRKD1 somatic mutations or PRKD gene family rearrangements harboured somatic mutations in the kinase domains of the PRKD2 or PRKD3 genes.
Title: Lack of PRKD2 and PRKD3 kinase domain somatic mutations in PRKD1 wild-type classic polymorphous low-grade adenocarcinomas of the salivary gland.

Studies indicate that the loss of protein kinase D PKD1 is thought to promote invasion and metastasis, while PKD2 and upregulated PKD3 to be positive regulators of proliferation.
Title: Functional and therapeutic significance of protein kinase D enzymes in invasive breast cancer.

the knockdown of PKD2 reduces cell death and promotes polyploidization induced by PMA. PMA/PKD2-mediated necrosis via PARP cleavage involves both SOD1-dependent and -independent pathways.
Title: Down-regulation of superoxide dismutase 1 by PMA is involved in cell fate determination and mediated via protein kinase D2 in myeloid leukemia cells.

The results implied that PKD2 silencing might inhibit migration of MCF7/DOX cells without affecting chemoresistance significantly
Title: Protein kinase D2 silencing reduced motility of doxorubicin-resistant MCF7 cells.

The following PRKD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02238	NM_001079880.1 Latest version!	Homo sapiens protein kinase D2 (PRKD2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu02238	NM_001079881.1 Latest version!	Homo sapiens protein kinase D2 (PRKD2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu02238	NM_016457.4 Latest version!	Homo sapiens protein kinase D2 (PRKD2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu05402	NM_001079882.1 Latest version!	Homo sapiens protein kinase D2 (PRKD2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu05402	XM_005258716.2 Latest version!	Homo sapiens protein kinase D2 (PRKD2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKD2 ( NM_016457.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057541.2 Homo sapiens protein kinase D2 (PRKD2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02238
Clone ID Related Accession (Same CDS sequence)	NM_016457.4 , NM_001079881.1 , NM_001079880.1
Accession Version	NM_016457.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase D2 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008635.6, AF309082.1 and AL050147.1. On Jan 4, 2007 this sequence version replaced NM_016457.3. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK092896.1, AF309082.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCACCG CCCCCTCTTA TCCCGCCGGG CTCCCTGGCT CTCCCGGGCC GGGGTCTCCT 
CCGCCCCCCG GCGGCCTAGA GCTGCAGTCG CCGCCACCGC TACTGCCCCA GATCCCGGCC 
CCGGGTTCCG GGGTCTCCTT TCACATCCAG ATCGGGCTGA CCCGCGAGTT CGTGCTGTTG 
CCCGCCGCCT CCGAGCTGGC TCATGTGAAG CAGCTGGCCT GTTCCATCGT GGACCAGAAG 
TTCCCTGAGT GTGGCTTCTA CGGCCTTTAC GACAAGATCC TGCTTTTCAA ACATGACCCC 
ACGTCGGCCA ACCTCCTGCA GCTGGTGCGC TCGTCCGGAG ACATCCAGGA GGGCGACCTG 
GTGGAGGTGG TGCTGTCGGC CTCGGCCACC TTCGAGGACT TCCAGATCCG CCCGCACGCC 
CTCACGGTGC ACTCCTATCG GGCGCCTGCC TTCTGTGATC ACTGCGGGGA GATGCTCTTC 
GGCCTAGTGC GCCAGGGCCT CAAGTGCGAT GGCTGCGGGC TGAACTACCA CAAGCGCTGT 
GCCTTCAGCA TCCCCAACAA CTGTAGTGGG GCCCGCAAAC GGCGCCTGTC ATCCACGTCT 
CTGGCCAGTG GCCACTCGGT GCGCCTCGGC ACCTCCGAGT CCCTGCCCTG CACGGCTGAA 
GAGCTGAGCC GTAGCACCAC CGAACTCCTG CCTCGCCGTC CCCCGTCATC CTCTTCCTCC 
TCTTCTGCCT CATCGTATAC GGGCCGCCCC ATTGAGCTGG ACAAGATGCT GCTCTCCAAG 
GTCAAGGTGC CGCACACCTT CCTCATCCAC AGCTATACAC GGCCCACCGT TTGCCAGGCT 
TGCAAGAAAC TCCTCAAGGG CCTCTTCCGG CAGGGCCTGC AATGCAAAGA CTGCAAGTTT 
AACTGTCACA AACGCTGCGC CACCCGCGTC CCTAATGACT GCCTGGGGGA GGCCCTTATC 
AATGGAGATG TGCCGATGGA GGAGGCCACC GATTTCAGCG AGGCTGACAA GAGCGCCCTC 
ATGGATGAGT CAGAGGACTC CGGTGTCATC CCTGGCTCCC ACTCAGAGAA TGCGCTCCAC 
GCCAGTGAGG AGGAGGAAGG CGAGGGAGGC AAGGCCCAGA GCTCCCTGGG GTACATCCCC 
CTAATGAGGG TGGTGCAATC GGTGCGACAC ACGACGCGGA AATCCAGCAC CACGCTGCGG 
GAGGGTTGGG TGGTTCATTA CAGCAACAAG GACACGCTGA GAAAGCGGCA CTATTGGCGC 
CTGGACTGCA AGTGTATCAC GCTCTTCCAG AACAACACGA CCAACAGATA CTATAAGGAA 
ATTCCGCTGT CAGAAATCCT CACGGTGGAG TCCGCCCAGA ACTTCAGCCT TGTGCCGCCG 
GGCACCAACC CACACTGCTT TGAGATCGTC ACTGCCAATG CCACCTACTT CGTGGGCGAG 
ATGCCTGGCG GGACTCCGGG TGGGCCAAGT GGGCAGGGGG CTGAGGCCGC CCGGGGCTGG 
GAGACAGCCA TCCGCCAGGC CCTGATGCCC GTCATCCTTC AGGACGCACC CAGCGCCCCA 
GGCCACGCGC CCCACAGACA AGCTTCTCTG AGCATCTCTG TGTCCAACAG TCAGATCCAA 
GAGAATGTGG ACATTGCCAC TGTCTACCAG ATCTTCCCTG ACGAAGTGCT GGGCTCAGGG 
CAGTTTGGAG TGGTCTATGG AGGAAAACAC CGGAAGACAG GCCGGGACGT GGCAGTTAAG 
GTCATTGACA AACTGCGCTT CCCTACCAAG CAGGAGAGCC AGCTCCGGAA TGAAGTGGCC 
ATTCTGCAGA GCCTGCGGCA TCCCGGGATC GTGAACCTGG AGTGCATGTT CGAGACGCCT 
GAGAAAGTGT TTGTGGTGAT GGAGAAGCTG CATGGGGACA TGTTGGAGAT GATCCTGTCC 
AGTGAGAAGG GCCGGCTGCC TGAGCGCCTC ACCAAGTTCC TCATCACCCA GATCCTGGTG 
GCTTTGAGAC ACCTTCACTT CAAGAACATT GTCCACTGTG ACTTGAAACC AGAAAACGTG 
TTGCTGGCAT CAGCAGACCC ATTTCCTCAG GTGAAGCTGT GTGACTTTGG CTTTGCTCGC 
ATCATCGGCG AGAAGTCGTT CCGCCGCTCA GTGGTGGGCA CGCCGGCCTA CCTGGCACCC 
GAGGTGCTGC TCAACCAGGG CTACAACCGC TCGCTGGACA TGTGGTCAGT GGGCGTGATC 
ATGTACGTCA GCCTCAGCGG CACCTTCCCT TTCAACGAGG ATGAGGACAT CAATGACCAG 
ATCCAGAACG CCGCCTTCAT GTACCCGGCC AGCCCCTGGA GCCACATCTC AGCTGGAGCC 
ATTGACCTCA TCAACAACCT GCTGCAGGTG AAGATGCGCA AACGCTACAG CGTGGACAAA 
TCTCTCAGCC ACCCCTGGTT ACAGGAGTAC CAGACGTGGC TGGACCTCCG AGAGCTGGAG 
GGGAAGATGG GAGAGCGATA CATCACGCAT GAGAGTGACG ACGCGCGCTG GGAGCAGTTT 
GCAGCAGAGC ATCCGCTGCC TGGGTCTGGG CTGCCCACGG ACAGGGATCT CGGTGGGGCC 
TGTCCACCAC AGGACCACGA CATGCAGGGG CTGGCGGAGC GCATCAGTGT TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057541.2
CDS	758..3394
Translation	MATAPSYPAGLPGSPGPGSPPPPGGLELQSPPPLLPQIPAPGSGVSFHIQIGLTREFVLLPAASELAHVKQLACSIVDQKFPECGFYGLYDKILLFKHDPTSANLLQLVRSSGDIQEGDLVEVVLSASATFEDFQIRPHALTVHSYRAPAFCDHCGEMLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTSLASGHSVRLGTSESLPCTAEELSRSTTELLPRRPPSSSSSSSASSYTGRPIELDKMLLSKVKVPHTFLIHSYTRPTVCQACKKLLKGLFRQGLQCKDCKFNCHKRCATRVPNDCLGEALINGDVPMEEATDFSEADKSALMDESEDSGVIPGSHSENALHASEEEEGEGGKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILTVESAQNFSLVPPGTNPHCFEIVTANATYFVGEMPGGTPGGPSGQGAEAARGWETAIRQALMPVILQDAPSAPGHAPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYPASPWSHISAGAIDLINNLLQVKMRKRYSVDKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWEQFAAEHPLPGSGLPTDRDLGGACPPQDHDMQGLAERISVL

Target ORF information:

RefSeq Version	NM_016457.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase D2 (PRKD2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016457.4

ORF Insert Sequence:

ATGGCCACCG CCCCCTCTTA TCCCGCCGGG CTCCCTGGCT CTCCCGGGCC GGGGTCTCCT 
CCGCCCCCCG GCGGCCTAGA GCTGCAGTCG CCGCCACCGC TACTGCCCCA GATCCCGGCC 
CCGGGTTCCG GGGTCTCCTT TCACATCCAG ATCGGGCTGA CCCGCGAGTT CGTGCTGTTG 
CCCGCCGCCT CCGAGCTGGC TCATGTGAAG CAGCTGGCCT GTTCCATCGT GGACCAGAAG 
TTCCCTGAGT GTGGCTTCTA CGGCCTTTAC GACAAGATCC TGCTTTTCAA ACATGACCCC 
ACGTCGGCCA ACCTCCTGCA GCTGGTGCGC TCGTCCGGAG ACATCCAGGA GGGCGACCTG 
GTGGAGGTGG TGCTGTCGGC CTCGGCCACC TTCGAGGACT TCCAGATCCG CCCGCACGCC 
CTCACGGTGC ACTCCTATCG GGCGCCTGCC TTCTGTGATC ACTGCGGGGA GATGCTCTTC 
GGCCTAGTGC GCCAGGGCCT CAAGTGCGAT GGCTGCGGGC TGAACTACCA CAAGCGCTGT 
GCCTTCAGCA TCCCCAACAA CTGTAGTGGG GCCCGCAAAC GGCGCCTGTC ATCCACGTCT 
CTGGCCAGTG GCCACTCGGT GCGCCTCGGC ACCTCCGAGT CCCTGCCCTG CACGGCTGAA 
GAGCTGAGCC GTAGCACCAC CGAACTCCTG CCTCGCCGTC CCCCGTCATC CTCTTCCTCC 
TCTTCTGCCT CATCGTATAC GGGCCGCCCC ATTGAGCTGG ACAAGATGCT GCTCTCCAAG 
GTCAAGGTGC CGCACACCTT CCTCATCCAC AGCTATACAC GGCCCACCGT TTGCCAGGCT 
TGCAAGAAAC TCCTCAAGGG CCTCTTCCGG CAGGGCCTGC AATGCAAAGA CTGCAAGTTT 
AACTGTCACA AACGCTGCGC CACCCGCGTC CCTAATGACT GCCTGGGGGA GGCCCTTATC 
AATGGAGATG TGCCGATGGA GGAGGCCACC GATTTCAGCG AGGCTGACAA GAGCGCCCTC 
ATGGATGAGT CAGAGGACTC CGGTGTCATC CCTGGCTCCC ACTCAGAGAA TGCGCTCCAC 
GCCAGTGAGG AGGAGGAAGG CGAGGGAGGC AAGGCCCAGA GCTCCCTGGG GTACATCCCC 
CTAATGAGGG TGGTGCAATC GGTGCGACAC ACGACGCGGA AATCCAGCAC CACGCTGCGG 
GAGGGTTGGG TGGTTCATTA CAGCAACAAG GACACGCTGA GAAAGCGGCA CTATTGGCGC 
CTGGACTGCA AGTGTATCAC GCTCTTCCAG AACAACACGA CCAACAGATA CTATAAGGAA 
ATTCCGCTGT CAGAAATCCT CACGGTGGAG TCCGCCCAGA ACTTCAGCCT TGTGCCGCCG 
GGCACCAACC CACACTGCTT TGAGATCGTC ACTGCCAATG CCACCTACTT CGTGGGCGAG 
ATGCCTGGCG GGACTCCGGG TGGGCCAAGT GGGCAGGGGG CTGAGGCCGC CCGGGGCTGG 
GAGACAGCCA TCCGCCAGGC CCTGATGCCC GTCATCCTTC AGGACGCACC CAGCGCCCCA 
GGCCACGCGC CCCACAGACA AGCTTCTCTG AGCATCTCTG TGTCCAACAG TCAGATCCAA 
GAGAATGTGG ACATTGCCAC TGTCTACCAG ATCTTCCCTG ACGAAGTGCT GGGCTCAGGG 
CAGTTTGGAG TGGTCTATGG AGGAAAACAC CGGAAGACAG GCCGGGACGT GGCAGTTAAG 
GTCATTGACA AACTGCGCTT CCCTACCAAG CAGGAGAGCC AGCTCCGGAA TGAAGTGGCC 
ATTCTGCAGA GCCTGCGGCA TCCCGGGATC GTGAACCTGG AGTGCATGTT CGAGACGCCT 
GAGAAAGTGT TTGTGGTGAT GGAGAAGCTG CATGGGGACA TGTTGGAGAT GATCCTGTCC 
AGTGAGAAGG GCCGGCTGCC TGAGCGCCTC ACCAAGTTCC TCATCACCCA GATCCTGGTG 
GCTTTGAGAC ACCTTCACTT CAAGAACATT GTCCACTGTG ACTTGAAACC AGAAAACGTG 
TTGCTGGCAT CAGCAGACCC ATTTCCTCAG GTGAAGCTGT GTGACTTTGG CTTTGCTCGC 
ATCATCGGCG AGAAGTCGTT CCGCCGCTCA GTGGTGGGCA CGCCGGCCTA CCTGGCACCC 
GAGGTGCTGC TCAACCAGGG CTACAACCGC TCGCTGGACA TGTGGTCAGT GGGCGTGATC 
ATGTACGTCA GCCTCAGCGG CACCTTCCCT TTCAACGAGG ATGAGGACAT CAATGACCAG 
ATCCAGAACG CCGCCTTCAT GTACCCGGCC AGCCCCTGGA GCCACATCTC AGCTGGAGCC 
ATTGACCTCA TCAACAACCT GCTGCAGGTG AAGATGCGCA AACGCTACAG CGTGGACAAA 
TCTCTCAGCC ACCCCTGGTT ACAGGAGTAC CAGACGTGGC TGGACCTCCG AGAGCTGGAG 
GGGAAGATGG GAGAGCGATA CATCACGCAT GAGAGTGACG ACGCGCGCTG GGAGCAGTTT 
GCAGCAGAGC ATCCGCTGCC TGGGTCTGGG CTGCCCACGG ACAGGGATCT CGGTGGGGCC 
TGTCCACCAC AGGACCACGA CATGCAGGGG CTGGCGGAGC GCATCAGTGT TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKD2 ( NM_001079881.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001073350.1 Homo sapiens protein kinase D2 (PRKD2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu02238
Clone ID Related Accession (Same CDS sequence)	NM_016457.4 , NM_001079881.1 , NM_001079880.1
Accession Version	NM_001079881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase D2 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008635.6, AF309082.1 and AL050147.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK092896.1, AF309082.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACCG CCCCCTCTTA TCCCGCCGGG CTCCCTGGCT CTCCCGGGCC GGGGTCTCCT 
CCGCCCCCCG GCGGCCTAGA GCTGCAGTCG CCGCCACCGC TACTGCCCCA GATCCCGGCC 
CCGGGTTCCG GGGTCTCCTT TCACATCCAG ATCGGGCTGA CCCGCGAGTT CGTGCTGTTG 
CCCGCCGCCT CCGAGCTGGC TCATGTGAAG CAGCTGGCCT GTTCCATCGT GGACCAGAAG 
TTCCCTGAGT GTGGCTTCTA CGGCCTTTAC GACAAGATCC TGCTTTTCAA ACATGACCCC 
ACGTCGGCCA ACCTCCTGCA GCTGGTGCGC TCGTCCGGAG ACATCCAGGA GGGCGACCTG 
GTGGAGGTGG TGCTGTCGGC CTCGGCCACC TTCGAGGACT TCCAGATCCG CCCGCACGCC 
CTCACGGTGC ACTCCTATCG GGCGCCTGCC TTCTGTGATC ACTGCGGGGA GATGCTCTTC 
GGCCTAGTGC GCCAGGGCCT CAAGTGCGAT GGCTGCGGGC TGAACTACCA CAAGCGCTGT 
GCCTTCAGCA TCCCCAACAA CTGTAGTGGG GCCCGCAAAC GGCGCCTGTC ATCCACGTCT 
CTGGCCAGTG GCCACTCGGT GCGCCTCGGC ACCTCCGAGT CCCTGCCCTG CACGGCTGAA 
GAGCTGAGCC GTAGCACCAC CGAACTCCTG CCTCGCCGTC CCCCGTCATC CTCTTCCTCC 
TCTTCTGCCT CATCGTATAC GGGCCGCCCC ATTGAGCTGG ACAAGATGCT GCTCTCCAAG 
GTCAAGGTGC CGCACACCTT CCTCATCCAC AGCTATACAC GGCCCACCGT TTGCCAGGCT 
TGCAAGAAAC TCCTCAAGGG CCTCTTCCGG CAGGGCCTGC AATGCAAAGA CTGCAAGTTT 
AACTGTCACA AACGCTGCGC CACCCGCGTC CCTAATGACT GCCTGGGGGA GGCCCTTATC 
AATGGAGATG TGCCGATGGA GGAGGCCACC GATTTCAGCG AGGCTGACAA GAGCGCCCTC 
ATGGATGAGT CAGAGGACTC CGGTGTCATC CCTGGCTCCC ACTCAGAGAA TGCGCTCCAC 
GCCAGTGAGG AGGAGGAAGG CGAGGGAGGC AAGGCCCAGA GCTCCCTGGG GTACATCCCC 
CTAATGAGGG TGGTGCAATC GGTGCGACAC ACGACGCGGA AATCCAGCAC CACGCTGCGG 
GAGGGTTGGG TGGTTCATTA CAGCAACAAG GACACGCTGA GAAAGCGGCA CTATTGGCGC 
CTGGACTGCA AGTGTATCAC GCTCTTCCAG AACAACACGA CCAACAGATA CTATAAGGAA 
ATTCCGCTGT CAGAAATCCT CACGGTGGAG TCCGCCCAGA ACTTCAGCCT TGTGCCGCCG 
GGCACCAACC CACACTGCTT TGAGATCGTC ACTGCCAATG CCACCTACTT CGTGGGCGAG 
ATGCCTGGCG GGACTCCGGG TGGGCCAAGT GGGCAGGGGG CTGAGGCCGC CCGGGGCTGG 
GAGACAGCCA TCCGCCAGGC CCTGATGCCC GTCATCCTTC AGGACGCACC CAGCGCCCCA 
GGCCACGCGC CCCACAGACA AGCTTCTCTG AGCATCTCTG TGTCCAACAG TCAGATCCAA 
GAGAATGTGG ACATTGCCAC TGTCTACCAG ATCTTCCCTG ACGAAGTGCT GGGCTCAGGG 
CAGTTTGGAG TGGTCTATGG AGGAAAACAC CGGAAGACAG GCCGGGACGT GGCAGTTAAG 
GTCATTGACA AACTGCGCTT CCCTACCAAG CAGGAGAGCC AGCTCCGGAA TGAAGTGGCC 
ATTCTGCAGA GCCTGCGGCA TCCCGGGATC GTGAACCTGG AGTGCATGTT CGAGACGCCT 
GAGAAAGTGT TTGTGGTGAT GGAGAAGCTG CATGGGGACA TGTTGGAGAT GATCCTGTCC 
AGTGAGAAGG GCCGGCTGCC TGAGCGCCTC ACCAAGTTCC TCATCACCCA GATCCTGGTG 
GCTTTGAGAC ACCTTCACTT CAAGAACATT GTCCACTGTG ACTTGAAACC AGAAAACGTG 
TTGCTGGCAT CAGCAGACCC ATTTCCTCAG GTGAAGCTGT GTGACTTTGG CTTTGCTCGC 
ATCATCGGCG AGAAGTCGTT CCGCCGCTCA GTGGTGGGCA CGCCGGCCTA CCTGGCACCC 
GAGGTGCTGC TCAACCAGGG CTACAACCGC TCGCTGGACA TGTGGTCAGT GGGCGTGATC 
ATGTACGTCA GCCTCAGCGG CACCTTCCCT TTCAACGAGG ATGAGGACAT CAATGACCAG 
ATCCAGAACG CCGCCTTCAT GTACCCGGCC AGCCCCTGGA GCCACATCTC AGCTGGAGCC 
ATTGACCTCA TCAACAACCT GCTGCAGGTG AAGATGCGCA AACGCTACAG CGTGGACAAA 
TCTCTCAGCC ACCCCTGGTT ACAGGAGTAC CAGACGTGGC TGGACCTCCG AGAGCTGGAG 
GGGAAGATGG GAGAGCGATA CATCACGCAT GAGAGTGACG ACGCGCGCTG GGAGCAGTTT 
GCAGCAGAGC ATCCGCTGCC TGGGTCTGGG CTGCCCACGG ACAGGGATCT CGGTGGGGCC 
TGTCCACCAC AGGACCACGA CATGCAGGGG CTGGCGGAGC GCATCAGTGT TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073350.1
CDS	343..2979
Translation	MATAPSYPAGLPGSPGPGSPPPPGGLELQSPPPLLPQIPAPGSGVSFHIQIGLTREFVLLPAASELAHVKQLACSIVDQKFPECGFYGLYDKILLFKHDPTSANLLQLVRSSGDIQEGDLVEVVLSASATFEDFQIRPHALTVHSYRAPAFCDHCGEMLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTSLASGHSVRLGTSESLPCTAEELSRSTTELLPRRPPSSSSSSSASSYTGRPIELDKMLLSKVKVPHTFLIHSYTRPTVCQACKKLLKGLFRQGLQCKDCKFNCHKRCATRVPNDCLGEALINGDVPMEEATDFSEADKSALMDESEDSGVIPGSHSENALHASEEEEGEGGKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILTVESAQNFSLVPPGTNPHCFEIVTANATYFVGEMPGGTPGGPSGQGAEAARGWETAIRQALMPVILQDAPSAPGHAPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYPASPWSHISAGAIDLINNLLQVKMRKRYSVDKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWEQFAAEHPLPGSGLPTDRDLGGACPPQDHDMQGLAERISVL

Target ORF information:

RefSeq Version	NM_001079881.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase D2 (PRKD2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079881.1

ORF Insert Sequence:

ATGGCCACCG CCCCCTCTTA TCCCGCCGGG CTCCCTGGCT CTCCCGGGCC GGGGTCTCCT 
CCGCCCCCCG GCGGCCTAGA GCTGCAGTCG CCGCCACCGC TACTGCCCCA GATCCCGGCC 
CCGGGTTCCG GGGTCTCCTT TCACATCCAG ATCGGGCTGA CCCGCGAGTT CGTGCTGTTG 
CCCGCCGCCT CCGAGCTGGC TCATGTGAAG CAGCTGGCCT GTTCCATCGT GGACCAGAAG 
TTCCCTGAGT GTGGCTTCTA CGGCCTTTAC GACAAGATCC TGCTTTTCAA ACATGACCCC 
ACGTCGGCCA ACCTCCTGCA GCTGGTGCGC TCGTCCGGAG ACATCCAGGA GGGCGACCTG 
GTGGAGGTGG TGCTGTCGGC CTCGGCCACC TTCGAGGACT TCCAGATCCG CCCGCACGCC 
CTCACGGTGC ACTCCTATCG GGCGCCTGCC TTCTGTGATC ACTGCGGGGA GATGCTCTTC 
GGCCTAGTGC GCCAGGGCCT CAAGTGCGAT GGCTGCGGGC TGAACTACCA CAAGCGCTGT 
GCCTTCAGCA TCCCCAACAA CTGTAGTGGG GCCCGCAAAC GGCGCCTGTC ATCCACGTCT 
CTGGCCAGTG GCCACTCGGT GCGCCTCGGC ACCTCCGAGT CCCTGCCCTG CACGGCTGAA 
GAGCTGAGCC GTAGCACCAC CGAACTCCTG CCTCGCCGTC CCCCGTCATC CTCTTCCTCC 
TCTTCTGCCT CATCGTATAC GGGCCGCCCC ATTGAGCTGG ACAAGATGCT GCTCTCCAAG 
GTCAAGGTGC CGCACACCTT CCTCATCCAC AGCTATACAC GGCCCACCGT TTGCCAGGCT 
TGCAAGAAAC TCCTCAAGGG CCTCTTCCGG CAGGGCCTGC AATGCAAAGA CTGCAAGTTT 
AACTGTCACA AACGCTGCGC CACCCGCGTC CCTAATGACT GCCTGGGGGA GGCCCTTATC 
AATGGAGATG TGCCGATGGA GGAGGCCACC GATTTCAGCG AGGCTGACAA GAGCGCCCTC 
ATGGATGAGT CAGAGGACTC CGGTGTCATC CCTGGCTCCC ACTCAGAGAA TGCGCTCCAC 
GCCAGTGAGG AGGAGGAAGG CGAGGGAGGC AAGGCCCAGA GCTCCCTGGG GTACATCCCC 
CTAATGAGGG TGGTGCAATC GGTGCGACAC ACGACGCGGA AATCCAGCAC CACGCTGCGG 
GAGGGTTGGG TGGTTCATTA CAGCAACAAG GACACGCTGA GAAAGCGGCA CTATTGGCGC 
CTGGACTGCA AGTGTATCAC GCTCTTCCAG AACAACACGA CCAACAGATA CTATAAGGAA 
ATTCCGCTGT CAGAAATCCT CACGGTGGAG TCCGCCCAGA ACTTCAGCCT TGTGCCGCCG 
GGCACCAACC CACACTGCTT TGAGATCGTC ACTGCCAATG CCACCTACTT CGTGGGCGAG 
ATGCCTGGCG GGACTCCGGG TGGGCCAAGT GGGCAGGGGG CTGAGGCCGC CCGGGGCTGG 
GAGACAGCCA TCCGCCAGGC CCTGATGCCC GTCATCCTTC AGGACGCACC CAGCGCCCCA 
GGCCACGCGC CCCACAGACA AGCTTCTCTG AGCATCTCTG TGTCCAACAG TCAGATCCAA 
GAGAATGTGG ACATTGCCAC TGTCTACCAG ATCTTCCCTG ACGAAGTGCT GGGCTCAGGG 
CAGTTTGGAG TGGTCTATGG AGGAAAACAC CGGAAGACAG GCCGGGACGT GGCAGTTAAG 
GTCATTGACA AACTGCGCTT CCCTACCAAG CAGGAGAGCC AGCTCCGGAA TGAAGTGGCC 
ATTCTGCAGA GCCTGCGGCA TCCCGGGATC GTGAACCTGG AGTGCATGTT CGAGACGCCT 
GAGAAAGTGT TTGTGGTGAT GGAGAAGCTG CATGGGGACA TGTTGGAGAT GATCCTGTCC 
AGTGAGAAGG GCCGGCTGCC TGAGCGCCTC ACCAAGTTCC TCATCACCCA GATCCTGGTG 
GCTTTGAGAC ACCTTCACTT CAAGAACATT GTCCACTGTG ACTTGAAACC AGAAAACGTG 
TTGCTGGCAT CAGCAGACCC ATTTCCTCAG GTGAAGCTGT GTGACTTTGG CTTTGCTCGC 
ATCATCGGCG AGAAGTCGTT CCGCCGCTCA GTGGTGGGCA CGCCGGCCTA CCTGGCACCC 
GAGGTGCTGC TCAACCAGGG CTACAACCGC TCGCTGGACA TGTGGTCAGT GGGCGTGATC 
ATGTACGTCA GCCTCAGCGG CACCTTCCCT TTCAACGAGG ATGAGGACAT CAATGACCAG 
ATCCAGAACG CCGCCTTCAT GTACCCGGCC AGCCCCTGGA GCCACATCTC AGCTGGAGCC 
ATTGACCTCA TCAACAACCT GCTGCAGGTG AAGATGCGCA AACGCTACAG CGTGGACAAA 
TCTCTCAGCC ACCCCTGGTT ACAGGAGTAC CAGACGTGGC TGGACCTCCG AGAGCTGGAG 
GGGAAGATGG GAGAGCGATA CATCACGCAT GAGAGTGACG ACGCGCGCTG GGAGCAGTTT 
GCAGCAGAGC ATCCGCTGCC TGGGTCTGGG CTGCCCACGG ACAGGGATCT CGGTGGGGCC 
TGTCCACCAC AGGACCACGA CATGCAGGGG CTGGCGGAGC GCATCAGTGT TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKD2 ( NM_001079880.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001073349.1 Homo sapiens protein kinase D2 (PRKD2), transcript variant 2, mRNA.

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CloneID	OHu02238
Clone ID Related Accession (Same CDS sequence)	NM_016457.4 , NM_001079881.1 , NM_001079880.1
Accession Version	NM_001079880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase D2 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008635.6, AF309082.1 and AL050147.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK092896.1, AF309082.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACCG CCCCCTCTTA TCCCGCCGGG CTCCCTGGCT CTCCCGGGCC GGGGTCTCCT 
CCGCCCCCCG GCGGCCTAGA GCTGCAGTCG CCGCCACCGC TACTGCCCCA GATCCCGGCC 
CCGGGTTCCG GGGTCTCCTT TCACATCCAG ATCGGGCTGA CCCGCGAGTT CGTGCTGTTG 
CCCGCCGCCT CCGAGCTGGC TCATGTGAAG CAGCTGGCCT GTTCCATCGT GGACCAGAAG 
TTCCCTGAGT GTGGCTTCTA CGGCCTTTAC GACAAGATCC TGCTTTTCAA ACATGACCCC 
ACGTCGGCCA ACCTCCTGCA GCTGGTGCGC TCGTCCGGAG ACATCCAGGA GGGCGACCTG 
GTGGAGGTGG TGCTGTCGGC CTCGGCCACC TTCGAGGACT TCCAGATCCG CCCGCACGCC 
CTCACGGTGC ACTCCTATCG GGCGCCTGCC TTCTGTGATC ACTGCGGGGA GATGCTCTTC 
GGCCTAGTGC GCCAGGGCCT CAAGTGCGAT GGCTGCGGGC TGAACTACCA CAAGCGCTGT 
GCCTTCAGCA TCCCCAACAA CTGTAGTGGG GCCCGCAAAC GGCGCCTGTC ATCCACGTCT 
CTGGCCAGTG GCCACTCGGT GCGCCTCGGC ACCTCCGAGT CCCTGCCCTG CACGGCTGAA 
GAGCTGAGCC GTAGCACCAC CGAACTCCTG CCTCGCCGTC CCCCGTCATC CTCTTCCTCC 
TCTTCTGCCT CATCGTATAC GGGCCGCCCC ATTGAGCTGG ACAAGATGCT GCTCTCCAAG 
GTCAAGGTGC CGCACACCTT CCTCATCCAC AGCTATACAC GGCCCACCGT TTGCCAGGCT 
TGCAAGAAAC TCCTCAAGGG CCTCTTCCGG CAGGGCCTGC AATGCAAAGA CTGCAAGTTT 
AACTGTCACA AACGCTGCGC CACCCGCGTC CCTAATGACT GCCTGGGGGA GGCCCTTATC 
AATGGAGATG TGCCGATGGA GGAGGCCACC GATTTCAGCG AGGCTGACAA GAGCGCCCTC 
ATGGATGAGT CAGAGGACTC CGGTGTCATC CCTGGCTCCC ACTCAGAGAA TGCGCTCCAC 
GCCAGTGAGG AGGAGGAAGG CGAGGGAGGC AAGGCCCAGA GCTCCCTGGG GTACATCCCC 
CTAATGAGGG TGGTGCAATC GGTGCGACAC ACGACGCGGA AATCCAGCAC CACGCTGCGG 
GAGGGTTGGG TGGTTCATTA CAGCAACAAG GACACGCTGA GAAAGCGGCA CTATTGGCGC 
CTGGACTGCA AGTGTATCAC GCTCTTCCAG AACAACACGA CCAACAGATA CTATAAGGAA 
ATTCCGCTGT CAGAAATCCT CACGGTGGAG TCCGCCCAGA ACTTCAGCCT TGTGCCGCCG 
GGCACCAACC CACACTGCTT TGAGATCGTC ACTGCCAATG CCACCTACTT CGTGGGCGAG 
ATGCCTGGCG GGACTCCGGG TGGGCCAAGT GGGCAGGGGG CTGAGGCCGC CCGGGGCTGG 
GAGACAGCCA TCCGCCAGGC CCTGATGCCC GTCATCCTTC AGGACGCACC CAGCGCCCCA 
GGCCACGCGC CCCACAGACA AGCTTCTCTG AGCATCTCTG TGTCCAACAG TCAGATCCAA 
GAGAATGTGG ACATTGCCAC TGTCTACCAG ATCTTCCCTG ACGAAGTGCT GGGCTCAGGG 
CAGTTTGGAG TGGTCTATGG AGGAAAACAC CGGAAGACAG GCCGGGACGT GGCAGTTAAG 
GTCATTGACA AACTGCGCTT CCCTACCAAG CAGGAGAGCC AGCTCCGGAA TGAAGTGGCC 
ATTCTGCAGA GCCTGCGGCA TCCCGGGATC GTGAACCTGG AGTGCATGTT CGAGACGCCT 
GAGAAAGTGT TTGTGGTGAT GGAGAAGCTG CATGGGGACA TGTTGGAGAT GATCCTGTCC 
AGTGAGAAGG GCCGGCTGCC TGAGCGCCTC ACCAAGTTCC TCATCACCCA GATCCTGGTG 
GCTTTGAGAC ACCTTCACTT CAAGAACATT GTCCACTGTG ACTTGAAACC AGAAAACGTG 
TTGCTGGCAT CAGCAGACCC ATTTCCTCAG GTGAAGCTGT GTGACTTTGG CTTTGCTCGC 
ATCATCGGCG AGAAGTCGTT CCGCCGCTCA GTGGTGGGCA CGCCGGCCTA CCTGGCACCC 
GAGGTGCTGC TCAACCAGGG CTACAACCGC TCGCTGGACA TGTGGTCAGT GGGCGTGATC 
ATGTACGTCA GCCTCAGCGG CACCTTCCCT TTCAACGAGG ATGAGGACAT CAATGACCAG 
ATCCAGAACG CCGCCTTCAT GTACCCGGCC AGCCCCTGGA GCCACATCTC AGCTGGAGCC 
ATTGACCTCA TCAACAACCT GCTGCAGGTG AAGATGCGCA AACGCTACAG CGTGGACAAA 
TCTCTCAGCC ACCCCTGGTT ACAGGAGTAC CAGACGTGGC TGGACCTCCG AGAGCTGGAG 
GGGAAGATGG GAGAGCGATA CATCACGCAT GAGAGTGACG ACGCGCGCTG GGAGCAGTTT 
GCAGCAGAGC ATCCGCTGCC TGGGTCTGGG CTGCCCACGG ACAGGGATCT CGGTGGGGCC 
TGTCCACCAC AGGACCACGA CATGCAGGGG CTGGCGGAGC GCATCAGTGT TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073349.1
CDS	479..3115
Translation	MATAPSYPAGLPGSPGPGSPPPPGGLELQSPPPLLPQIPAPGSGVSFHIQIGLTREFVLLPAASELAHVKQLACSIVDQKFPECGFYGLYDKILLFKHDPTSANLLQLVRSSGDIQEGDLVEVVLSASATFEDFQIRPHALTVHSYRAPAFCDHCGEMLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTSLASGHSVRLGTSESLPCTAEELSRSTTELLPRRPPSSSSSSSASSYTGRPIELDKMLLSKVKVPHTFLIHSYTRPTVCQACKKLLKGLFRQGLQCKDCKFNCHKRCATRVPNDCLGEALINGDVPMEEATDFSEADKSALMDESEDSGVIPGSHSENALHASEEEEGEGGKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILTVESAQNFSLVPPGTNPHCFEIVTANATYFVGEMPGGTPGGPSGQGAEAARGWETAIRQALMPVILQDAPSAPGHAPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYPASPWSHISAGAIDLINNLLQVKMRKRYSVDKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWEQFAAEHPLPGSGLPTDRDLGGACPPQDHDMQGLAERISVL

Target ORF information:

RefSeq Version	NM_001079880.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase D2 (PRKD2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079880.1

ORF Insert Sequence:

ATGGCCACCG CCCCCTCTTA TCCCGCCGGG CTCCCTGGCT CTCCCGGGCC GGGGTCTCCT 
CCGCCCCCCG GCGGCCTAGA GCTGCAGTCG CCGCCACCGC TACTGCCCCA GATCCCGGCC 
CCGGGTTCCG GGGTCTCCTT TCACATCCAG ATCGGGCTGA CCCGCGAGTT CGTGCTGTTG 
CCCGCCGCCT CCGAGCTGGC TCATGTGAAG CAGCTGGCCT GTTCCATCGT GGACCAGAAG 
TTCCCTGAGT GTGGCTTCTA CGGCCTTTAC GACAAGATCC TGCTTTTCAA ACATGACCCC 
ACGTCGGCCA ACCTCCTGCA GCTGGTGCGC TCGTCCGGAG ACATCCAGGA GGGCGACCTG 
GTGGAGGTGG TGCTGTCGGC CTCGGCCACC TTCGAGGACT TCCAGATCCG CCCGCACGCC 
CTCACGGTGC ACTCCTATCG GGCGCCTGCC TTCTGTGATC ACTGCGGGGA GATGCTCTTC 
GGCCTAGTGC GCCAGGGCCT CAAGTGCGAT GGCTGCGGGC TGAACTACCA CAAGCGCTGT 
GCCTTCAGCA TCCCCAACAA CTGTAGTGGG GCCCGCAAAC GGCGCCTGTC ATCCACGTCT 
CTGGCCAGTG GCCACTCGGT GCGCCTCGGC ACCTCCGAGT CCCTGCCCTG CACGGCTGAA 
GAGCTGAGCC GTAGCACCAC CGAACTCCTG CCTCGCCGTC CCCCGTCATC CTCTTCCTCC 
TCTTCTGCCT CATCGTATAC GGGCCGCCCC ATTGAGCTGG ACAAGATGCT GCTCTCCAAG 
GTCAAGGTGC CGCACACCTT CCTCATCCAC AGCTATACAC GGCCCACCGT TTGCCAGGCT 
TGCAAGAAAC TCCTCAAGGG CCTCTTCCGG CAGGGCCTGC AATGCAAAGA CTGCAAGTTT 
AACTGTCACA AACGCTGCGC CACCCGCGTC CCTAATGACT GCCTGGGGGA GGCCCTTATC 
AATGGAGATG TGCCGATGGA GGAGGCCACC GATTTCAGCG AGGCTGACAA GAGCGCCCTC 
ATGGATGAGT CAGAGGACTC CGGTGTCATC CCTGGCTCCC ACTCAGAGAA TGCGCTCCAC 
GCCAGTGAGG AGGAGGAAGG CGAGGGAGGC AAGGCCCAGA GCTCCCTGGG GTACATCCCC 
CTAATGAGGG TGGTGCAATC GGTGCGACAC ACGACGCGGA AATCCAGCAC CACGCTGCGG 
GAGGGTTGGG TGGTTCATTA CAGCAACAAG GACACGCTGA GAAAGCGGCA CTATTGGCGC 
CTGGACTGCA AGTGTATCAC GCTCTTCCAG AACAACACGA CCAACAGATA CTATAAGGAA 
ATTCCGCTGT CAGAAATCCT CACGGTGGAG TCCGCCCAGA ACTTCAGCCT TGTGCCGCCG 
GGCACCAACC CACACTGCTT TGAGATCGTC ACTGCCAATG CCACCTACTT CGTGGGCGAG 
ATGCCTGGCG GGACTCCGGG TGGGCCAAGT GGGCAGGGGG CTGAGGCCGC CCGGGGCTGG 
GAGACAGCCA TCCGCCAGGC CCTGATGCCC GTCATCCTTC AGGACGCACC CAGCGCCCCA 
GGCCACGCGC CCCACAGACA AGCTTCTCTG AGCATCTCTG TGTCCAACAG TCAGATCCAA 
GAGAATGTGG ACATTGCCAC TGTCTACCAG ATCTTCCCTG ACGAAGTGCT GGGCTCAGGG 
CAGTTTGGAG TGGTCTATGG AGGAAAACAC CGGAAGACAG GCCGGGACGT GGCAGTTAAG 
GTCATTGACA AACTGCGCTT CCCTACCAAG CAGGAGAGCC AGCTCCGGAA TGAAGTGGCC 
ATTCTGCAGA GCCTGCGGCA TCCCGGGATC GTGAACCTGG AGTGCATGTT CGAGACGCCT 
GAGAAAGTGT TTGTGGTGAT GGAGAAGCTG CATGGGGACA TGTTGGAGAT GATCCTGTCC 
AGTGAGAAGG GCCGGCTGCC TGAGCGCCTC ACCAAGTTCC TCATCACCCA GATCCTGGTG 
GCTTTGAGAC ACCTTCACTT CAAGAACATT GTCCACTGTG ACTTGAAACC AGAAAACGTG 
TTGCTGGCAT CAGCAGACCC ATTTCCTCAG GTGAAGCTGT GTGACTTTGG CTTTGCTCGC 
ATCATCGGCG AGAAGTCGTT CCGCCGCTCA GTGGTGGGCA CGCCGGCCTA CCTGGCACCC 
GAGGTGCTGC TCAACCAGGG CTACAACCGC TCGCTGGACA TGTGGTCAGT GGGCGTGATC 
ATGTACGTCA GCCTCAGCGG CACCTTCCCT TTCAACGAGG ATGAGGACAT CAATGACCAG 
ATCCAGAACG CCGCCTTCAT GTACCCGGCC AGCCCCTGGA GCCACATCTC AGCTGGAGCC 
ATTGACCTCA TCAACAACCT GCTGCAGGTG AAGATGCGCA AACGCTACAG CGTGGACAAA 
TCTCTCAGCC ACCCCTGGTT ACAGGAGTAC CAGACGTGGC TGGACCTCCG AGAGCTGGAG 
GGGAAGATGG GAGAGCGATA CATCACGCAT GAGAGTGACG ACGCGCGCTG GGAGCAGTTT 
GCAGCAGAGC ATCCGCTGCC TGGGTCTGGG CTGCCCACGG ACAGGGATCT CGGTGGGGCC 
TGTCCACCAC AGGACCACGA CATGCAGGGG CTGGCGGAGC GCATCAGTGT TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKD2 ( NM_001079882.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001073351.1 Homo sapiens protein kinase D2 (PRKD2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu05402
Clone ID Related Accession (Same CDS sequence)	NM_001079882.1 , XM_005258716.2
Accession Version	NM_001079882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase D2 isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA475821.1, AF309082.1 and AL050147.1. Transcript Variant: This variant (4) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (B) has a shorter N-terminus when compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK075266.1, SRR1660809.27682.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCTTCG GCCTAGTGCG CCAGGGCCTC AAGTGCGATG GCTGCGGGCT GAACTACCAC 
AAGCGCTGTG CCTTCAGCAT CCCCAACAAC TGTAGTGGGG CCCGCAAACG GCGCCTGTCA 
TCCACGTCTC TGGCCAGTGG CCACTCGGTG CGCCTCGGCA CCTCCGAGTC CCTGCCCTGC 
ACGGCTGAAG AGCTGAGCCG TAGCACCACC GAACTCCTGC CTCGCCGTCC CCCGTCATCC 
TCTTCCTCCT CTTCTGCCTC ATCGTATACG GGCCGCCCCA TTGAGCTGGA CAAGATGCTG 
CTCTCCAAGG TCAAGGTGCC GCACACCTTC CTCATCCACA GCTATACACG GCCCACCGTT 
TGCCAGGCTT GCAAGAAACT CCTCAAGGGC CTCTTCCGGC AGGGCCTGCA ATGCAAAGAC 
TGCAAGTTTA ACTGTCACAA ACGCTGCGCC ACCCGCGTCC CTAATGACTG CCTGGGGGAG 
GCCCTTATCA ATGGAGATGT GCCGATGGAG GAGGCCACCG ATTTCAGCGA GGCTGACAAG 
AGCGCCCTCA TGGATGAGTC AGAGGACTCC GGTGTCATCC CTGGCTCCCA CTCAGAGAAT 
GCGCTCCACG CCAGTGAGGA GGAGGAAGGC GAGGGAGGCA AGGCCCAGAG CTCCCTGGGG 
TACATCCCCC TAATGAGGGT GGTGCAATCG GTGCGACACA CGACGCGGAA ATCCAGCACC 
ACGCTGCGGG AGGGTTGGGT GGTTCATTAC AGCAACAAGG ACACGCTGAG AAAGCGGCAC 
TATTGGCGCC TGGACTGCAA GTGTATCACG CTCTTCCAGA ACAACACGAC CAACAGATAC 
TATAAGGAAA TTCCGCTGTC AGAAATCCTC ACGGTGGAGT CCGCCCAGAA CTTCAGCCTT 
GTGCCGCCGG GCACCAACCC ACACTGCTTT GAGATCGTCA CTGCCAATGC CACCTACTTC 
GTGGGCGAGA TGCCTGGCGG GACTCCGGGT GGGCCAAGTG GGCAGGGGGC TGAGGCCGCC 
CGGGGCTGGG AGACAGCCAT CCGCCAGGCC CTGATGCCCG TCATCCTTCA GGACGCACCC 
AGCGCCCCAG GCCACGCGCC CCACAGACAA GCTTCTCTGA GCATCTCTGT GTCCAACAGT 
CAGATCCAAG AGAATGTGGA CATTGCCACT GTCTACCAGA TCTTCCCTGA CGAAGTGCTG 
GGCTCAGGGC AGTTTGGAGT GGTCTATGGA GGAAAACACC GGAAGACAGG CCGGGACGTG 
GCAGTTAAGG TCATTGACAA ACTGCGCTTC CCTACCAAGC AGGAGAGCCA GCTCCGGAAT 
GAAGTGGCCA TTCTGCAGAG CCTGCGGCAT CCCGGGATCG TGAACCTGGA GTGCATGTTC 
GAGACGCCTG AGAAAGTGTT TGTGGTGATG GAGAAGCTGC ATGGGGACAT GTTGGAGATG 
ATCCTGTCCA GTGAGAAGGG CCGGCTGCCT GAGCGCCTCA CCAAGTTCCT CATCACCCAG 
ATCCTGGTGG CTTTGAGACA CCTTCACTTC AAGAACATTG TCCACTGTGA CTTGAAACCA 
GAAAACGTGT TGCTGGCATC AGCAGACCCA TTTCCTCAGG TGAAGCTGTG TGACTTTGGC 
TTTGCTCGCA TCATCGGCGA GAAGTCGTTC CGCCGCTCAG TGGTGGGCAC GCCGGCCTAC 
CTGGCACCCG AGGTGCTGCT CAACCAGGGC TACAACCGCT CGCTGGACAT GTGGTCAGTG 
GGCGTGATCA TGTACGTCAG CCTCAGCGGC ACCTTCCCTT TCAACGAGGA TGAGGACATC 
AATGACCAGA TCCAGAACGC CGCCTTCATG TACCCGGCCA GCCCCTGGAG CCACATCTCA 
GCTGGAGCCA TTGACCTCAT CAACAACCTG CTGCAGGTGA AGATGCGCAA ACGCTACAGC 
GTGGACAAAT CTCTCAGCCA CCCCTGGTTA CAGGAGTACC AGACGTGGCT GGACCTCCGA 
GAGCTGGAGG GGAAGATGGG AGAGCGATAC ATCACGCATG AGAGTGACGA CGCGCGCTGG 
GAGCAGTTTG CAGCAGAGCA TCCGCTGCCT GGGTCTGGGC TGCCCACGGA CAGGGATCTC 
GGTGGGGCCT GTCCACCACA GGACCACGAC ATGCAGGGGC TGGCGGAGCG CATCAGTGTT 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073351.1
CDS	551..2716
Translation	MLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTSLASGHSVRLGTSESLPCTAEELSRSTTELLPRRPPSSSSSSSASSYTGRPIELDKMLLSKVKVPHTFLIHSYTRPTVCQACKKLLKGLFRQGLQCKDCKFNCHKRCATRVPNDCLGEALINGDVPMEEATDFSEADKSALMDESEDSGVIPGSHSENALHASEEEEGEGGKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILTVESAQNFSLVPPGTNPHCFEIVTANATYFVGEMPGGTPGGPSGQGAEAARGWETAIRQALMPVILQDAPSAPGHAPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYPASPWSHISAGAIDLINNLLQVKMRKRYSVDKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWEQFAAEHPLPGSGLPTDRDLGGACPPQDHDMQGLAERISVL

Target ORF information:

RefSeq Version	NM_001079882.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase D2 (PRKD2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079882.1

ORF Insert Sequence:

ATGCTCTTCG GCCTAGTGCG CCAGGGCCTC AAGTGCGATG GCTGCGGGCT GAACTACCAC 
AAGCGCTGTG CCTTCAGCAT CCCCAACAAC TGTAGTGGGG CCCGCAAACG GCGCCTGTCA 
TCCACGTCTC TGGCCAGTGG CCACTCGGTG CGCCTCGGCA CCTCCGAGTC CCTGCCCTGC 
ACGGCTGAAG AGCTGAGCCG TAGCACCACC GAACTCCTGC CTCGCCGTCC CCCGTCATCC 
TCTTCCTCCT CTTCTGCCTC ATCGTATACG GGCCGCCCCA TTGAGCTGGA CAAGATGCTG 
CTCTCCAAGG TCAAGGTGCC GCACACCTTC CTCATCCACA GCTATACACG GCCCACCGTT 
TGCCAGGCTT GCAAGAAACT CCTCAAGGGC CTCTTCCGGC AGGGCCTGCA ATGCAAAGAC 
TGCAAGTTTA ACTGTCACAA ACGCTGCGCC ACCCGCGTCC CTAATGACTG CCTGGGGGAG 
GCCCTTATCA ATGGAGATGT GCCGATGGAG GAGGCCACCG ATTTCAGCGA GGCTGACAAG 
AGCGCCCTCA TGGATGAGTC AGAGGACTCC GGTGTCATCC CTGGCTCCCA CTCAGAGAAT 
GCGCTCCACG CCAGTGAGGA GGAGGAAGGC GAGGGAGGCA AGGCCCAGAG CTCCCTGGGG 
TACATCCCCC TAATGAGGGT GGTGCAATCG GTGCGACACA CGACGCGGAA ATCCAGCACC 
ACGCTGCGGG AGGGTTGGGT GGTTCATTAC AGCAACAAGG ACACGCTGAG AAAGCGGCAC 
TATTGGCGCC TGGACTGCAA GTGTATCACG CTCTTCCAGA ACAACACGAC CAACAGATAC 
TATAAGGAAA TTCCGCTGTC AGAAATCCTC ACGGTGGAGT CCGCCCAGAA CTTCAGCCTT 
GTGCCGCCGG GCACCAACCC ACACTGCTTT GAGATCGTCA CTGCCAATGC CACCTACTTC 
GTGGGCGAGA TGCCTGGCGG GACTCCGGGT GGGCCAAGTG GGCAGGGGGC TGAGGCCGCC 
CGGGGCTGGG AGACAGCCAT CCGCCAGGCC CTGATGCCCG TCATCCTTCA GGACGCACCC 
AGCGCCCCAG GCCACGCGCC CCACAGACAA GCTTCTCTGA GCATCTCTGT GTCCAACAGT 
CAGATCCAAG AGAATGTGGA CATTGCCACT GTCTACCAGA TCTTCCCTGA CGAAGTGCTG 
GGCTCAGGGC AGTTTGGAGT GGTCTATGGA GGAAAACACC GGAAGACAGG CCGGGACGTG 
GCAGTTAAGG TCATTGACAA ACTGCGCTTC CCTACCAAGC AGGAGAGCCA GCTCCGGAAT 
GAAGTGGCCA TTCTGCAGAG CCTGCGGCAT CCCGGGATCG TGAACCTGGA GTGCATGTTC 
GAGACGCCTG AGAAAGTGTT TGTGGTGATG GAGAAGCTGC ATGGGGACAT GTTGGAGATG 
ATCCTGTCCA GTGAGAAGGG CCGGCTGCCT GAGCGCCTCA CCAAGTTCCT CATCACCCAG 
ATCCTGGTGG CTTTGAGACA CCTTCACTTC AAGAACATTG TCCACTGTGA CTTGAAACCA 
GAAAACGTGT TGCTGGCATC AGCAGACCCA TTTCCTCAGG TGAAGCTGTG TGACTTTGGC 
TTTGCTCGCA TCATCGGCGA GAAGTCGTTC CGCCGCTCAG TGGTGGGCAC GCCGGCCTAC 
CTGGCACCCG AGGTGCTGCT CAACCAGGGC TACAACCGCT CGCTGGACAT GTGGTCAGTG 
GGCGTGATCA TGTACGTCAG CCTCAGCGGC ACCTTCCCTT TCAACGAGGA TGAGGACATC 
AATGACCAGA TCCAGAACGC CGCCTTCATG TACCCGGCCA GCCCCTGGAG CCACATCTCA 
GCTGGAGCCA TTGACCTCAT CAACAACCTG CTGCAGGTGA AGATGCGCAA ACGCTACAGC 
GTGGACAAAT CTCTCAGCCA CCCCTGGTTA CAGGAGTACC AGACGTGGCT GGACCTCCGA 
GAGCTGGAGG GGAAGATGGG AGAGCGATAC ATCACGCATG AGAGTGACGA CGCGCGCTGG 
GAGCAGTTTG CAGCAGAGCA TCCGCTGCCT GGGTCTGGGC TGCCCACGGA CAGGGATCTC 
GGTGGGGCCT GTCCACCACA GGACCACGAC ATGCAGGGGC TGGCGGAGCG CATCAGTGTT 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKD2 ( XM_005258716.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258773.2 Homo sapiens protein kinase D2 (PRKD2), transcript variant X1, mRNA.

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CloneID	OHu05402
Clone ID Related Accession (Same CDS sequence)	NM_001079882.1 , XM_005258716.2
Accession Version	XM_005258716.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase D2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005258716.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTCG GCCTAGTGCG CCAGGGCCTC AAGTGCGATG GCTGCGGGCT GAACTACCAC 
AAGCGCTGTG CCTTCAGCAT CCCCAACAAC TGTAGTGGGG CCCGCAAACG GCGCCTGTCA 
TCCACGTCTC TGGCCAGTGG CCACTCGGTG CGCCTCGGCA CCTCCGAGTC CCTGCCCTGC 
ACGGCTGAAG AGCTGAGCCG TAGCACCACC GAACTCCTGC CTCGCCGTCC CCCGTCATCC 
TCTTCCTCCT CTTCTGCCTC ATCGTATACG GGCCGCCCCA TTGAGCTGGA CAAGATGCTG 
CTCTCCAAGG TCAAGGTGCC GCACACCTTC CTCATCCACA GCTATACACG GCCCACCGTT 
TGCCAGGCTT GCAAGAAACT CCTCAAGGGC CTCTTCCGGC AGGGCCTGCA ATGCAAAGAC 
TGCAAGTTTA ACTGTCACAA ACGCTGCGCC ACCCGCGTCC CTAATGACTG CCTGGGGGAG 
GCCCTTATCA ATGGAGATGT GCCGATGGAG GAGGCCACCG ATTTCAGCGA GGCTGACAAG 
AGCGCCCTCA TGGATGAGTC AGAGGACTCC GGTGTCATCC CTGGCTCCCA CTCAGAGAAT 
GCGCTCCACG CCAGTGAGGA GGAGGAAGGC GAGGGAGGCA AGGCCCAGAG CTCCCTGGGG 
TACATCCCCC TAATGAGGGT GGTGCAATCG GTGCGACACA CGACGCGGAA ATCCAGCACC 
ACGCTGCGGG AGGGTTGGGT GGTTCATTAC AGCAACAAGG ACACGCTGAG AAAGCGGCAC 
TATTGGCGCC TGGACTGCAA GTGTATCACG CTCTTCCAGA ACAACACGAC CAACAGATAC 
TATAAGGAAA TTCCGCTGTC AGAAATCCTC ACGGTGGAGT CCGCCCAGAA CTTCAGCCTT 
GTGCCGCCGG GCACCAACCC ACACTGCTTT GAGATCGTCA CTGCCAATGC CACCTACTTC 
GTGGGCGAGA TGCCTGGCGG GACTCCGGGT GGGCCAAGTG GGCAGGGGGC TGAGGCCGCC 
CGGGGCTGGG AGACAGCCAT CCGCCAGGCC CTGATGCCCG TCATCCTTCA GGACGCACCC 
AGCGCCCCAG GCCACGCGCC CCACAGACAA GCTTCTCTGA GCATCTCTGT GTCCAACAGT 
CAGATCCAAG AGAATGTGGA CATTGCCACT GTCTACCAGA TCTTCCCTGA CGAAGTGCTG 
GGCTCAGGGC AGTTTGGAGT GGTCTATGGA GGAAAACACC GGAAGACAGG CCGGGACGTG 
GCAGTTAAGG TCATTGACAA ACTGCGCTTC CCTACCAAGC AGGAGAGCCA GCTCCGGAAT 
GAAGTGGCCA TTCTGCAGAG CCTGCGGCAT CCCGGGATCG TGAACCTGGA GTGCATGTTC 
GAGACGCCTG AGAAAGTGTT TGTGGTGATG GAGAAGCTGC ATGGGGACAT GTTGGAGATG 
ATCCTGTCCA GTGAGAAGGG CCGGCTGCCT GAGCGCCTCA CCAAGTTCCT CATCACCCAG 
ATCCTGGTGG CTTTGAGACA CCTTCACTTC AAGAACATTG TCCACTGTGA CTTGAAACCA 
GAAAACGTGT TGCTGGCATC AGCAGACCCA TTTCCTCAGG TGAAGCTGTG TGACTTTGGC 
TTTGCTCGCA TCATCGGCGA GAAGTCGTTC CGCCGCTCAG TGGTGGGCAC GCCGGCCTAC 
CTGGCACCCG AGGTGCTGCT CAACCAGGGC TACAACCGCT CGCTGGACAT GTGGTCAGTG 
GGCGTGATCA TGTACGTCAG CCTCAGCGGC ACCTTCCCTT TCAACGAGGA TGAGGACATC 
AATGACCAGA TCCAGAACGC CGCCTTCATG TACCCGGCCA GCCCCTGGAG CCACATCTCA 
GCTGGAGCCA TTGACCTCAT CAACAACCTG CTGCAGGTGA AGATGCGCAA ACGCTACAGC 
GTGGACAAAT CTCTCAGCCA CCCCTGGTTA CAGGAGTACC AGACGTGGCT GGACCTCCGA 
GAGCTGGAGG GGAAGATGGG AGAGCGATAC ATCACGCATG AGAGTGACGA CGCGCGCTGG 
GAGCAGTTTG CAGCAGAGCA TCCGCTGCCT GGGTCTGGGC TGCCCACGGA CAGGGATCTC 
GGTGGGGCCT GTCCACCACA GGACCACGAC ATGCAGGGGC TGGCGGAGCG CATCAGTGTT 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258773.2
CDS	207..2372
Translation	MLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTSLASGHSVRLGTSESLPCTAEELSRSTTELLPRRPPSSSSSSSASSYTGRPIELDKMLLSKVKVPHTFLIHSYTRPTVCQACKKLLKGLFRQGLQCKDCKFNCHKRCATRVPNDCLGEALINGDVPMEEATDFSEADKSALMDESEDSGVIPGSHSENALHASEEEEGEGGKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILTVESAQNFSLVPPGTNPHCFEIVTANATYFVGEMPGGTPGGPSGQGAEAARGWETAIRQALMPVILQDAPSAPGHAPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYPASPWSHISAGAIDLINNLLQVKMRKRYSVDKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWEQFAAEHPLPGSGLPTDRDLGGACPPQDHDMQGLAERISVL

Target ORF information:

RefSeq Version	XM_005258716.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase D2 (PRKD2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005258716.2

ORF Insert Sequence:

ATGCTCTTCG GCCTAGTGCG CCAGGGCCTC AAGTGCGATG GCTGCGGGCT GAACTACCAC 
AAGCGCTGTG CCTTCAGCAT CCCCAACAAC TGTAGTGGGG CCCGCAAACG GCGCCTGTCA 
TCCACGTCTC TGGCCAGTGG CCACTCGGTG CGCCTCGGCA CCTCCGAGTC CCTGCCCTGC 
ACGGCTGAAG AGCTGAGCCG TAGCACCACC GAACTCCTGC CTCGCCGTCC CCCGTCATCC 
TCTTCCTCCT CTTCTGCCTC ATCGTATACG GGCCGCCCCA TTGAGCTGGA CAAGATGCTG 
CTCTCCAAGG TCAAGGTGCC GCACACCTTC CTCATCCACA GCTATACACG GCCCACCGTT 
TGCCAGGCTT GCAAGAAACT CCTCAAGGGC CTCTTCCGGC AGGGCCTGCA ATGCAAAGAC 
TGCAAGTTTA ACTGTCACAA ACGCTGCGCC ACCCGCGTCC CTAATGACTG CCTGGGGGAG 
GCCCTTATCA ATGGAGATGT GCCGATGGAG GAGGCCACCG ATTTCAGCGA GGCTGACAAG 
AGCGCCCTCA TGGATGAGTC AGAGGACTCC GGTGTCATCC CTGGCTCCCA CTCAGAGAAT 
GCGCTCCACG CCAGTGAGGA GGAGGAAGGC GAGGGAGGCA AGGCCCAGAG CTCCCTGGGG 
TACATCCCCC TAATGAGGGT GGTGCAATCG GTGCGACACA CGACGCGGAA ATCCAGCACC 
ACGCTGCGGG AGGGTTGGGT GGTTCATTAC AGCAACAAGG ACACGCTGAG AAAGCGGCAC 
TATTGGCGCC TGGACTGCAA GTGTATCACG CTCTTCCAGA ACAACACGAC CAACAGATAC 
TATAAGGAAA TTCCGCTGTC AGAAATCCTC ACGGTGGAGT CCGCCCAGAA CTTCAGCCTT 
GTGCCGCCGG GCACCAACCC ACACTGCTTT GAGATCGTCA CTGCCAATGC CACCTACTTC 
GTGGGCGAGA TGCCTGGCGG GACTCCGGGT GGGCCAAGTG GGCAGGGGGC TGAGGCCGCC 
CGGGGCTGGG AGACAGCCAT CCGCCAGGCC CTGATGCCCG TCATCCTTCA GGACGCACCC 
AGCGCCCCAG GCCACGCGCC CCACAGACAA GCTTCTCTGA GCATCTCTGT GTCCAACAGT 
CAGATCCAAG AGAATGTGGA CATTGCCACT GTCTACCAGA TCTTCCCTGA CGAAGTGCTG 
GGCTCAGGGC AGTTTGGAGT GGTCTATGGA GGAAAACACC GGAAGACAGG CCGGGACGTG 
GCAGTTAAGG TCATTGACAA ACTGCGCTTC CCTACCAAGC AGGAGAGCCA GCTCCGGAAT 
GAAGTGGCCA TTCTGCAGAG CCTGCGGCAT CCCGGGATCG TGAACCTGGA GTGCATGTTC 
GAGACGCCTG AGAAAGTGTT TGTGGTGATG GAGAAGCTGC ATGGGGACAT GTTGGAGATG 
ATCCTGTCCA GTGAGAAGGG CCGGCTGCCT GAGCGCCTCA CCAAGTTCCT CATCACCCAG 
ATCCTGGTGG CTTTGAGACA CCTTCACTTC AAGAACATTG TCCACTGTGA CTTGAAACCA 
GAAAACGTGT TGCTGGCATC AGCAGACCCA TTTCCTCAGG TGAAGCTGTG TGACTTTGGC 
TTTGCTCGCA TCATCGGCGA GAAGTCGTTC CGCCGCTCAG TGGTGGGCAC GCCGGCCTAC 
CTGGCACCCG AGGTGCTGCT CAACCAGGGC TACAACCGCT CGCTGGACAT GTGGTCAGTG 
GGCGTGATCA TGTACGTCAG CCTCAGCGGC ACCTTCCCTT TCAACGAGGA TGAGGACATC 
AATGACCAGA TCCAGAACGC CGCCTTCATG TACCCGGCCA GCCCCTGGAG CCACATCTCA 
GCTGGAGCCA TTGACCTCAT CAACAACCTG CTGCAGGTGA AGATGCGCAA ACGCTACAGC 
GTGGACAAAT CTCTCAGCCA CCCCTGGTTA CAGGAGTACC AGACGTGGCT GGACCTCCGA 
GAGCTGGAGG GGAAGATGGG AGAGCGATAC ATCACGCATG AGAGTGACGA CGCGCGCTGG 
GAGCAGTTTG CAGCAGAGCA TCCGCTGCCT GGGTCTGGGC TGCCCACGGA CAGGGATCTC 
GGTGGGGCCT GTCCACCACA GGACCACGAC ATGCAGGGGC TGGCGGAGCG CATCAGTGTT 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein kinase D2: a versatile player in cancer biology.
Oncogene(2017 Dec)
Azoitei N,Cobbaut M,Becher A,Van Lint J,Seufferlein T

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Differential regulation of PKD isoforms in oxidative stress conditions through phosphorylation of a conserved Tyr in the P+1 loop.
Scientific reports7(1)887(2017 Apr)
Cobbaut M,Derua R,D?ppler H,Lou HJ,Vandoninck S,Storz P,Turk BE,Seufferlein T,Waelkens E,Janssens V,Van Lint J

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PRKD2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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