Prkd2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkd2
Entrez Gene ID	101540
Full Name	protein kinase D2
Synonyms	AI325941,PKD2
General protein information	Preferred Names protein kinase D2 Names serine/threonine-protein kinase D2 nPKC-D2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001252458.1	NP_001239387.1	serine/threonine-protein kinase D2
NM_178900.4	NP_849231.1	serine/threonine-protein kinase D2
XM_006539444.1	XP_006539507.1	serine/threonine-protein kinase D2 isoform X1
XM_006539444.2	XP_006539507.1	serine/threonine-protein kinase D2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRKD2	NP_057541.2
Pan troglodytes (chimpanzee)	PRKD2	XP_524314.3
Rattus norvegicus (Norway rat)	Prkd2	NP_001013917.1
Mus musculus (house mouse)	Prkd2	NP_001239387.1
Bos taurus (cattle)	PRKD2	XP_005219507.1
Canis lupus familiaris (dog)	PRKD2	XP_005616468.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein kinase D regulates positive selection of CD4
Ishikawa E, Kosako H, Yasuda T, Ohmuraya M, Araki K, Kurosaki T, Saito T, Yamasaki S
Nature communications712756(2016 Sep)

Protein kinase D1/2 is involved in the maturation of multivesicular bodies and secretion of exosomes in T and B lymphocytes.
Mazzeo C, Calvo V, Alonso R, M?rida I, Izquierdo M
Cell death and differentiation23(1)99-109(2016 Jan)

Functional redundancy of protein kinase D1 and protein kinase D2 in neuronal polarity.
Avriyanti E, Atik N, Kunii M, Furumoto N, Iwano T, Yoshimura S, Harada R, Harada A
Neuroscience research9512-20(2015 Jun)

Protein kinase D2 is a digital amplifier of T cell receptor-stimulated diacylglycerol signaling in na?ve CD8? T cells.
Navarro MN, Feijoo-Carnero C, Arandilla AG, Trost M, Cantrell DA
Science signaling7(348)ra99(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our studies demonstrate that PKD1/2 is a key regulator of MVB maturation and exosome secretion, and constitutes a mediator of the DGK alpha effect on MVB secretory traffic.
Title: Protein kinase D1/2 is involved in the maturation of multivesicular bodies and secretion of exosomes in T and B lymphocytes.

Results reveal that whereas protein kinase D1 and protein kinase D2 are essential for neuronal polarity, there exists a functional redundancy between the two proteins.
Title: Functional redundancy of protein kinase D1 and protein kinase D2 in neuronal polarity.

PKD2 acted as an amplification checkpoint for antigen-stimulated digital cytokine responses and translated the differential strength of TCR signaling to determine the number of naive CD8(+) T cells that became effector cells.
Title: Protein kinase D2 is a digital amplifier of T cell receptor-stimulated diacylglycerol signaling in na?ve CD8? T cells.

Protein kinase D2 promotes in vitro osteoclast differentiation and fusion
Title: Protein kinase D promotes in vitro osteoclast differentiation and fusion.

A bioinformatic screen identified the serine-threonine kinase protein kinase D2 (PRKD2) as a potential effector of GABP in hematopoietic stem cells
Title: GABP transcription factor is required for development of chronic myelogenous leukemia via its control of PRKD2.

The following Prkd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19152	NM_001252458.1 Latest version!	Mus musculus protein kinase D2 (Prkd2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu19152	NM_178900.4 Latest version!	Mus musculus protein kinase D2 (Prkd2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu37943	XM_006539444.2 Latest version!	Mus musculus protein kinase D2 (Prkd2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu37943	XM_006539444.1	Mus musculus protein kinase D2 (Prkd2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkd2 ( XM_006539444.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539507.1 Mus musculus protein kinase D2 (Prkd2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu37943
Clone ID Related Accession (Same CDS sequence)	XM_006539444.1 , XM_006539444.2
Accession Version	XM_006539444.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase D2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAG CCGCTGATTA CAGTGAGGCT GATAAGAGCT CCATCTCAGA TGAGTTGGAG 
GATTCTGGTG TCATCCCCGG CTCCCACTCA GAGAGTGCTC TCCATGCCAG TGAGGAAGAG 
GAAGGCGAGG GACACAAGGC CCAGAGCTCG CTGGGATACA TCCCCCTGAT GCGCGTGGTA 
CAGTCTGTGC GACACACGAC CCGGAAATCC AGCACCACCC TGCGGGAGGG CTGGGTGGTC 
CATTACAGCA ACAAGGACAC ACTGAGGAAA CGGCACTACT GGCGTCTAGA CTGTAAATGC 
ATTACCCTCT TCCAGAACAA CACGACCAAC AGATACTACA AGGAAATCCC GCTGTCTGAA 
ATCCTTGCGG TGGAACCGGC TCAGAACTTC AGCCTCGTAC CCCCGGGCAC CAACCCACAC 
TGCTTCGAGA TCATCACGGC CAATGTCACC TACTTTGTGG GCGAAACCCC TGGCGGGGCC 
CCGGGAGGGC CAAGTGGACA GGGAACAGAG GCCGTTCGGG GTTGGGAGAC AGCCATCCGC 
CAGGCACTGA TGCCCGTTAT CCTCCAAGAT GCGCCCAGTG CCCCGGGGCA CACACCGCAC 
AGACAAGCGT CTCTGAGTAT CTCCGTGTCC AACAGTCAGA TCCAAGAGAA TGTGGATATC 
GCCACTGTCT ACCAGATCTT CCCAGATGAG GTGCTGGGCT CTGGGCAGTT TGGAGTGGTT 
TATGGAGGAA AACACAGGAA GACTGGCAGA GACGTTGCAG TAAAGGTCAT TGACAAACTG 
CGCTTCCCCA CCAAGCAGGA GAGCCAGCTC AGGAATGAAG TGGCCATTCT CCAGAGTCTA 
CGGCACCCCG GGATTGTGAA CCTCGAGTGC ATGTTTGAGA CTCCTGAGAA GGTATTCGTG 
GTGATGGAGA AACTGCACGG GGACATGTTG GAGATGATCC TCTCCAGTGA GAAGGGCCGG 
CTTCCTGAGC GTCTCACCAA GTTCCTTATC ACGCAGATCC TGGTGGCGCT GAGACACCTG 
CACTTCAAGA ACATCGTCCA CTGTGACTTG AAACCAGAGA ATGTGTTACT GGCATCAGCC 
GATCCGTTTC CTCAGGTGAA GCTCTGTGAC TTTGGCTTCG CTCGCATCAT CGGCGAGAAG 
TCCTTCCGGC GCTCAGTGGT GGGCACGCCT GCCTACCTGG CACCCGAGGT ACTGCTCAAC 
CAGGGTTACA ACCGCTCGCT GGACATGTGG TCCGTGGGTG TGATCATGTA CGTGAGCCTT 
AGTGGCACGT TCCCCTTCAA TGAGGACGAG GACATCAATG ACCAGATACA GAACGCGGCC 
TTTATGTACC CGGCCAGCCC CTGGAGCCAC ATCTCATCTG GAGCCATCGA CCTCATCAAC 
AACCTGTTGC AGGTGAAGAT GCGCAAGCGC TACAGCGTGG ACAAGTCTCT CAGCCACCCA 
TGGTTACAGG AGTACCAGAC GTGGCTTGAC CTCCGAGAGC TAGAGGGAAA GATGGGCGAG 
CGATATATCA CGCACGAGAG CGACGACGCA CGCTGGGATC AGTTTGTGGC AGAGCGCCAT 
GGGACTCCTG CAGAAGGGGA CCTGGGTGGC GCCTGTCTGC CGCAGGACCA CGAGATGCAG 
GGGCTGGCTG AGCGCATCAG CATCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539507.1
CDS	92..1741
Translation	MEEAADYSEADKSSISDELEDSGVIPGSHSESALHASEEEEGEGHKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILAVEPAQNFSLVPPGTNPHCFEIITANVTYFVGETPGGAPGGPSGQGTEAVRGWETAIRQALMPVILQDAPSAPGHTPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYPASPWSHISSGAIDLINNLLQVKMRKRYSVDKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWDQFVAERHGTPAEGDLGGACLPQDHEMQGLAERISIL

Target ORF information:

RefSeq Version	XM_006539444.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase D2 (Prkd2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539444.1

ORF Insert Sequence:

ATGGAGGAAG CCGCTGATTA CAGTGAGGCT GATAAGAGCT CCATCTCAGA TGAGTTGGAG 
GATTCTGGTG TCATCCCCGG CTCCCACTCA GAGAGTGCTC TCCATGCCAG TGAGGAAGAG 
GAAGGCGAGG GACACAAGGC CCAGAGCTCG CTGGGATACA TCCCCCTGAT GCGCGTGGTA 
CAGTCTGTGC GACACACGAC CCGGAAATCC AGCACCACCC TGCGGGAGGG CTGGGTGGTC 
CATTACAGCA ACAAGGACAC ACTGAGGAAA CGGCACTACT GGCGTCTAGA CTGTAAATGC 
ATTACCCTCT TCCAGAACAA CACGACCAAC AGATACTACA AGGAAATCCC GCTGTCTGAA 
ATCCTTGCGG TGGAACCGGC TCAGAACTTC AGCCTCGTAC CCCCGGGCAC CAACCCACAC 
TGCTTCGAGA TCATCACGGC CAATGTCACC TACTTTGTGG GCGAAACCCC TGGCGGGGCC 
CCGGGAGGGC CAAGTGGACA GGGAACAGAG GCCGTTCGGG GTTGGGAGAC AGCCATCCGC 
CAGGCACTGA TGCCCGTTAT CCTCCAAGAT GCGCCCAGTG CCCCGGGGCA CACACCGCAC 
AGACAAGCGT CTCTGAGTAT CTCCGTGTCC AACAGTCAGA TCCAAGAGAA TGTGGATATC 
GCCACTGTCT ACCAGATCTT CCCAGATGAG GTGCTGGGCT CTGGGCAGTT TGGAGTGGTT 
TATGGAGGAA AACACAGGAA GACTGGCAGA GACGTTGCAG TAAAGGTCAT TGACAAACTG 
CGCTTCCCCA CCAAGCAGGA GAGCCAGCTC AGGAATGAAG TGGCCATTCT CCAGAGTCTA 
CGGCACCCCG GGATTGTGAA CCTCGAGTGC ATGTTTGAGA CTCCTGAGAA GGTATTCGTG 
GTGATGGAGA AACTGCACGG GGACATGTTG GAGATGATCC TCTCCAGTGA GAAGGGCCGG 
CTTCCTGAGC GTCTCACCAA GTTCCTTATC ACGCAGATCC TGGTGGCGCT GAGACACCTG 
CACTTCAAGA ACATCGTCCA CTGTGACTTG AAACCAGAGA ATGTGTTACT GGCATCAGCC 
GATCCGTTTC CTCAGGTGAA GCTCTGTGAC TTTGGCTTCG CTCGCATCAT CGGCGAGAAG 
TCCTTCCGGC GCTCAGTGGT GGGCACGCCT GCCTACCTGG CACCCGAGGT ACTGCTCAAC 
CAGGGTTACA ACCGCTCGCT GGACATGTGG TCCGTGGGTG TGATCATGTA CGTGAGCCTT 
AGTGGCACGT TCCCCTTCAA TGAGGACGAG GACATCAATG ACCAGATACA GAACGCGGCC 
TTTATGTACC CGGCCAGCCC CTGGAGCCAC ATCTCATCTG GAGCCATCGA CCTCATCAAC 
AACCTGTTGC AGGTGAAGAT GCGCAAGCGC TACAGCGTGG ACAAGTCTCT CAGCCACCCA 
TGGTTACAGG AGTACCAGAC GTGGCTTGAC CTCCGAGAGC TAGAGGGAAA GATGGGCGAG 
CGATATATCA CGCACGAGAG CGACGACGCA CGCTGGGATC AGTTTGTGGC AGAGCGCCAT 
GGGACTCCTG CAGAAGGGGA CCTGGGTGGC GCCTGTCTGC CGCAGGACCA CGAGATGCAG 
GGGCTGGCTG AGCGCATCAG CATCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkd2 ( NM_001252458.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239387.1 Mus musculus protein kinase D2 (Prkd2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19152
Clone ID Related Accession (Same CDS sequence)	NM_001252458.1 , NM_178900.4
Accession Version	NM_001252458.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase D2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY202739.1, AK037030.1 and BF146730.1. Transcript Variant: This variant (2) has a different 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC096444.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGCCG CCCCCTCCCA TCCCGCAGGA CTCCCCGGCT CTCCGGGGCC CGGGTCTCCT 
CCGCCCCCCG GTGGCTTGGA TCTCCAGTCG CCGCCACCTC TGCTGCCTCA GATCCCGGCT 
CCGGGTTCGG GGGTCTCCTT CCATATCCAG ATCGGATTGA CCCGAGAGTT CGTGCTGTTG 
CCGGCTGCCT CAGAGTTGGC TCATGTGAAG CAACTAGCCT GTTCTATCGT GGACCAGAAG 
TTCCCAGAGT GTGGCTTCTA CGGCCTGTAC GACAAGATCC TGCTCTTCAA ACATGACCCA 
ACATCAGCCA ACCTCCTGCA GCTGGTGCGA TCAGCTGCAG ATATCCAGGA GGGTGACCTG 
GTGGAGGTGG TTCTGTCGGC CTCGGCCACC TTCGAGGATT TCCAGATCCG ACCGCATGCT 
CTCACTGTGC ACTCGTACCG CGCTCCCGCC TTCTGTGACC ACTGCGGGGA GATGCTCTTC 
GGCCTCGTGC GCCAGGGCCT CAAGTGCGAT GGCTGCGGGC TGAATTACCA CAAACGCTGC 
GCCTTCAGCA TCCCGAACAA CTGCAGTGGT GCTCGAAAGC GCCGCCTGTC ATCCACGTCT 
CTGGCCAGTG GCCATTCTGT GCGCCTTGGC AGCTCCGAGT CCTTACCCTG CACCGCCGAA 
GAGCTGAGCC GTAGCACCAC CGACCTCCTT CCTCGCCGAC CACCCTCGTC GTCGTCCTCC 
TCCTCTTCCT CTTCCTTCTA CACAGGCCGA CCCATTGAGT TGGACAAGAT GCTGATGTCC 
AAGGTCAAGG TGCCACACAC CTTCCTTATA CATAGCTACA CACGGCCCAC CGTTTGCCAG 
GCTTGCAAGA AACTGCTCAA GGGTCTATTC CGCCAGGGCC TGCAGTGCAA AGACTGCAAG 
TTTAACTGTC ACAAACGCTG TGCTACCCGC GTCCCTAACG ACTGCCTGGG GGAGGCGCTC 
ATCAATGGAG ACGTGCCGAT GGAGGAAGCC GCTGATTACA GTGAGGCTGA TAAGAGCTCC 
ATCTCAGATG AGTTGGAGGA TTCTGGTGTC ATCCCCGGCT CCCACTCAGA GAGTGCTCTC 
CATGCCAGTG AGGAAGAGGA AGGCGAGGGA CACAAGGCCC AGAGCTCGCT GGGATACATC 
CCCCTGATGC GCGTGGTACA GTCTGTGCGA CACACGACCC GGAAATCCAG CACCACCCTG 
CGGGAGGGCT GGGTGGTCCA TTACAGCAAC AAGGACACAC TGAGGAAACG GCACTACTGG 
CGTCTAGACT GTAAATGCAT TACCCTCTTC CAGAACAACA CGACCAACAG ATACTACAAG 
GAAATCCCGC TGTCTGAAAT CCTTGCGGTG GAACCGGCTC AGAACTTCAG CCTCGTACCC 
CCGGGCACCA ACCCACACTG CTTCGAGATC ATCACGGCCA ATGTCACCTA CTTTGTGGGC 
GAAACCCCTG GCGGGGCCCC GGGAGGGCCA AGTGGACAGG GAACAGAGGC CGTTCGGGGT 
TGGGAGACAG CCATCCGCCA GGCACTGATG CCCGTTATCC TCCAAGATGC GCCCAGTGCC 
CCGGGGCACA CACCGCACAG ACAAGCGTCT CTGAGTATCT CCGTGTCCAA CAGTCAGATC 
CAAGAGAATG TGGATATCGC CACTGTCTAC CAGATCTTCC CAGATGAGGT GCTGGGCTCT 
GGGCAGTTTG GAGTGGTTTA TGGAGGAAAA CACAGGAAGA CTGGCAGAGA CGTTGCAGTA 
AAGGTCATTG ACAAACTGCG CTTCCCCACC AAGCAGGAGA GCCAGCTCAG GAATGAAGTG 
GCCATTCTCC AGAGTCTACG GCACCCCGGG ATTGTGAACC TCGAGTGCAT GTTTGAGACT 
CCTGAGAAGG TATTCGTGGT GATGGAGAAA CTGCACGGGG ACATGTTGGA GATGATCCTC 
TCCAGTGAGA AGGGCCGGCT TCCTGAGCGT CTCACCAAGT TCCTTATCAC GCAGATCCTG 
GTGGCGCTGA GACACCTGCA CTTCAAGAAC ATCGTCCACT GTGACTTGAA ACCAGAGAAT 
GTGTTACTGG CATCAGCCGA TCCGTTTCCT CAGGTGAAGC TCTGTGACTT TGGCTTCGCT 
CGCATCATCG GCGAGAAGTC CTTCCGGCGC TCAGTGGTGG GCACGCCTGC CTACCTGGCA 
CCCGAGGTAC TGCTCAACCA GGGTTACAAC CGCTCGCTGG ACATGTGGTC CGTGGGTGTG 
ATCATGTACG TGAGCCTTAG TGGCACGTTC CCCTTCAATG AGGACGAGGA CATCAATGAC 
CAGATACAGA ACGCGGCCTT TATGTACCCG GCCAGCCCCT GGAGCCACAT CTCATCTGGA 
GCCATCGACC TCATCAACAA CCTGTTGCAG GTGAAGATGC GCAAGCGCTA CAGCGTGGAC 
AAGTCTCTCA GCCACCCATG GTTACAGGAG TACCAGACGT GGCTTGACCT CCGAGAGCTA 
GAGGGAAAGA TGGGCGAGCG ATATATCACG CACGAGAGCG ACGACGCACG CTGGGATCAG 
TTTGTGGCAG AGCGCCATGG GACTCCTGCA GAAGGGGACC TGGGTGGCGC CTGTCTGCCG 
CAGGACCACG AGATGCAGGG GCTGGCTGAG CGCATCAGCA TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239387.1
CDS	275..2902
Translation	MAAAPSHPAGLPGSPGPGSPPPPGGLDLQSPPPLLPQIPAPGSGVSFHIQIGLTREFVLLPAASELAHVKQLACSIVDQKFPECGFYGLYDKILLFKHDPTSANLLQLVRSAADIQEGDLVEVVLSASATFEDFQIRPHALTVHSYRAPAFCDHCGEMLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTSLASGHSVRLGSSESLPCTAEELSRSTTDLLPRRPPSSSSSSSSSSFYTGRPIELDKMLMSKVKVPHTFLIHSYTRPTVCQACKKLLKGLFRQGLQCKDCKFNCHKRCATRVPNDCLGEALINGDVPMEEAADYSEADKSSISDELEDSGVIPGSHSESALHASEEEEGEGHKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILAVEPAQNFSLVPPGTNPHCFEIITANVTYFVGETPGGAPGGPSGQGTEAVRGWETAIRQALMPVILQDAPSAPGHTPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYPASPWSHISSGAIDLINNLLQVKMRKRYSVDKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWDQFVAERHGTPAEGDLGGACLPQDHEMQGLAERISIL

Target ORF information:

RefSeq Version	NM_001252458.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase D2 (Prkd2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252458.1

ORF Insert Sequence:

ATGGCCGCCG CCCCCTCCCA TCCCGCAGGA CTCCCCGGCT CTCCGGGGCC CGGGTCTCCT 
CCGCCCCCCG GTGGCTTGGA TCTCCAGTCG CCGCCACCTC TGCTGCCTCA GATCCCGGCT 
CCGGGTTCGG GGGTCTCCTT CCATATCCAG ATCGGATTGA CCCGAGAGTT CGTGCTGTTG 
CCGGCTGCCT CAGAGTTGGC TCATGTGAAG CAACTAGCCT GTTCTATCGT GGACCAGAAG 
TTCCCAGAGT GTGGCTTCTA CGGCCTGTAC GACAAGATCC TGCTCTTCAA ACATGACCCA 
ACATCAGCCA ACCTCCTGCA GCTGGTGCGA TCAGCTGCAG ATATCCAGGA GGGTGACCTG 
GTGGAGGTGG TTCTGTCGGC CTCGGCCACC TTCGAGGATT TCCAGATCCG ACCGCATGCT 
CTCACTGTGC ACTCGTACCG CGCTCCCGCC TTCTGTGACC ACTGCGGGGA GATGCTCTTC 
GGCCTCGTGC GCCAGGGCCT CAAGTGCGAT GGCTGCGGGC TGAATTACCA CAAACGCTGC 
GCCTTCAGCA TCCCGAACAA CTGCAGTGGT GCTCGAAAGC GCCGCCTGTC ATCCACGTCT 
CTGGCCAGTG GCCATTCTGT GCGCCTTGGC AGCTCCGAGT CCTTACCCTG CACCGCCGAA 
GAGCTGAGCC GTAGCACCAC CGACCTCCTT CCTCGCCGAC CACCCTCGTC GTCGTCCTCC 
TCCTCTTCCT CTTCCTTCTA CACAGGCCGA CCCATTGAGT TGGACAAGAT GCTGATGTCC 
AAGGTCAAGG TGCCACACAC CTTCCTTATA CATAGCTACA CACGGCCCAC CGTTTGCCAG 
GCTTGCAAGA AACTGCTCAA GGGTCTATTC CGCCAGGGCC TGCAGTGCAA AGACTGCAAG 
TTTAACTGTC ACAAACGCTG TGCTACCCGC GTCCCTAACG ACTGCCTGGG GGAGGCGCTC 
ATCAATGGAG ACGTGCCGAT GGAGGAAGCC GCTGATTACA GTGAGGCTGA TAAGAGCTCC 
ATCTCAGATG AGTTGGAGGA TTCTGGTGTC ATCCCCGGCT CCCACTCAGA GAGTGCTCTC 
CATGCCAGTG AGGAAGAGGA AGGCGAGGGA CACAAGGCCC AGAGCTCGCT GGGATACATC 
CCCCTGATGC GCGTGGTACA GTCTGTGCGA CACACGACCC GGAAATCCAG CACCACCCTG 
CGGGAGGGCT GGGTGGTCCA TTACAGCAAC AAGGACACAC TGAGGAAACG GCACTACTGG 
CGTCTAGACT GTAAATGCAT TACCCTCTTC CAGAACAACA CGACCAACAG ATACTACAAG 
GAAATCCCGC TGTCTGAAAT CCTTGCGGTG GAACCGGCTC AGAACTTCAG CCTCGTACCC 
CCGGGCACCA ACCCACACTG CTTCGAGATC ATCACGGCCA ATGTCACCTA CTTTGTGGGC 
GAAACCCCTG GCGGGGCCCC GGGAGGGCCA AGTGGACAGG GAACAGAGGC CGTTCGGGGT 
TGGGAGACAG CCATCCGCCA GGCACTGATG CCCGTTATCC TCCAAGATGC GCCCAGTGCC 
CCGGGGCACA CACCGCACAG ACAAGCGTCT CTGAGTATCT CCGTGTCCAA CAGTCAGATC 
CAAGAGAATG TGGATATCGC CACTGTCTAC CAGATCTTCC CAGATGAGGT GCTGGGCTCT 
GGGCAGTTTG GAGTGGTTTA TGGAGGAAAA CACAGGAAGA CTGGCAGAGA CGTTGCAGTA 
AAGGTCATTG ACAAACTGCG CTTCCCCACC AAGCAGGAGA GCCAGCTCAG GAATGAAGTG 
GCCATTCTCC AGAGTCTACG GCACCCCGGG ATTGTGAACC TCGAGTGCAT GTTTGAGACT 
CCTGAGAAGG TATTCGTGGT GATGGAGAAA CTGCACGGGG ACATGTTGGA GATGATCCTC 
TCCAGTGAGA AGGGCCGGCT TCCTGAGCGT CTCACCAAGT TCCTTATCAC GCAGATCCTG 
GTGGCGCTGA GACACCTGCA CTTCAAGAAC ATCGTCCACT GTGACTTGAA ACCAGAGAAT 
GTGTTACTGG CATCAGCCGA TCCGTTTCCT CAGGTGAAGC TCTGTGACTT TGGCTTCGCT 
CGCATCATCG GCGAGAAGTC CTTCCGGCGC TCAGTGGTGG GCACGCCTGC CTACCTGGCA 
CCCGAGGTAC TGCTCAACCA GGGTTACAAC CGCTCGCTGG ACATGTGGTC CGTGGGTGTG 
ATCATGTACG TGAGCCTTAG TGGCACGTTC CCCTTCAATG AGGACGAGGA CATCAATGAC 
CAGATACAGA ACGCGGCCTT TATGTACCCG GCCAGCCCCT GGAGCCACAT CTCATCTGGA 
GCCATCGACC TCATCAACAA CCTGTTGCAG GTGAAGATGC GCAAGCGCTA CAGCGTGGAC 
AAGTCTCTCA GCCACCCATG GTTACAGGAG TACCAGACGT GGCTTGACCT CCGAGAGCTA 
GAGGGAAAGA TGGGCGAGCG ATATATCACG CACGAGAGCG ACGACGCACG CTGGGATCAG 
TTTGTGGCAG AGCGCCATGG GACTCCTGCA GAAGGGGACC TGGGTGGCGC CTGTCTGCCG 
CAGGACCACG AGATGCAGGG GCTGGCTGAG CGCATCAGCA TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkd2 ( NM_178900.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_849231.1 Mus musculus protein kinase D2 (Prkd2), transcript variant 1, mRNA.

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CloneID	OMu19152
Clone ID Related Accession (Same CDS sequence)	NM_001252458.1 , NM_178900.4
Accession Version	NM_178900.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase D2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK037030.1 and BF146730.1. On May 2, 2007 this sequence version replaced NM_178900.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK037030.1, AK154029.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGCCG CCCCCTCCCA TCCCGCAGGA CTCCCCGGCT CTCCGGGGCC CGGGTCTCCT 
CCGCCCCCCG GTGGCTTGGA TCTCCAGTCG CCGCCACCTC TGCTGCCTCA GATCCCGGCT 
CCGGGTTCGG GGGTCTCCTT CCATATCCAG ATCGGATTGA CCCGAGAGTT CGTGCTGTTG 
CCGGCTGCCT CAGAGTTGGC TCATGTGAAG CAACTAGCCT GTTCTATCGT GGACCAGAAG 
TTCCCAGAGT GTGGCTTCTA CGGCCTGTAC GACAAGATCC TGCTCTTCAA ACATGACCCA 
ACATCAGCCA ACCTCCTGCA GCTGGTGCGA TCAGCTGCAG ATATCCAGGA GGGTGACCTG 
GTGGAGGTGG TTCTGTCGGC CTCGGCCACC TTCGAGGATT TCCAGATCCG ACCGCATGCT 
CTCACTGTGC ACTCGTACCG CGCTCCCGCC TTCTGTGACC ACTGCGGGGA GATGCTCTTC 
GGCCTCGTGC GCCAGGGCCT CAAGTGCGAT GGCTGCGGGC TGAATTACCA CAAACGCTGC 
GCCTTCAGCA TCCCGAACAA CTGCAGTGGT GCTCGAAAGC GCCGCCTGTC ATCCACGTCT 
CTGGCCAGTG GCCATTCTGT GCGCCTTGGC AGCTCCGAGT CCTTACCCTG CACCGCCGAA 
GAGCTGAGCC GTAGCACCAC CGACCTCCTT CCTCGCCGAC CACCCTCGTC GTCGTCCTCC 
TCCTCTTCCT CTTCCTTCTA CACAGGCCGA CCCATTGAGT TGGACAAGAT GCTGATGTCC 
AAGGTCAAGG TGCCACACAC CTTCCTTATA CATAGCTACA CACGGCCCAC CGTTTGCCAG 
GCTTGCAAGA AACTGCTCAA GGGTCTATTC CGCCAGGGCC TGCAGTGCAA AGACTGCAAG 
TTTAACTGTC ACAAACGCTG TGCTACCCGC GTCCCTAACG ACTGCCTGGG GGAGGCGCTC 
ATCAATGGAG ACGTGCCGAT GGAGGAAGCC GCTGATTACA GTGAGGCTGA TAAGAGCTCC 
ATCTCAGATG AGTTGGAGGA TTCTGGTGTC ATCCCCGGCT CCCACTCAGA GAGTGCTCTC 
CATGCCAGTG AGGAAGAGGA AGGCGAGGGA CACAAGGCCC AGAGCTCGCT GGGATACATC 
CCCCTGATGC GCGTGGTACA GTCTGTGCGA CACACGACCC GGAAATCCAG CACCACCCTG 
CGGGAGGGCT GGGTGGTCCA TTACAGCAAC AAGGACACAC TGAGGAAACG GCACTACTGG 
CGTCTAGACT GTAAATGCAT TACCCTCTTC CAGAACAACA CGACCAACAG ATACTACAAG 
GAAATCCCGC TGTCTGAAAT CCTTGCGGTG GAACCGGCTC AGAACTTCAG CCTCGTACCC 
CCGGGCACCA ACCCACACTG CTTCGAGATC ATCACGGCCA ATGTCACCTA CTTTGTGGGC 
GAAACCCCTG GCGGGGCCCC GGGAGGGCCA AGTGGACAGG GAACAGAGGC CGTTCGGGGT 
TGGGAGACAG CCATCCGCCA GGCACTGATG CCCGTTATCC TCCAAGATGC GCCCAGTGCC 
CCGGGGCACA CACCGCACAG ACAAGCGTCT CTGAGTATCT CCGTGTCCAA CAGTCAGATC 
CAAGAGAATG TGGATATCGC CACTGTCTAC CAGATCTTCC CAGATGAGGT GCTGGGCTCT 
GGGCAGTTTG GAGTGGTTTA TGGAGGAAAA CACAGGAAGA CTGGCAGAGA CGTTGCAGTA 
AAGGTCATTG ACAAACTGCG CTTCCCCACC AAGCAGGAGA GCCAGCTCAG GAATGAAGTG 
GCCATTCTCC AGAGTCTACG GCACCCCGGG ATTGTGAACC TCGAGTGCAT GTTTGAGACT 
CCTGAGAAGG TATTCGTGGT GATGGAGAAA CTGCACGGGG ACATGTTGGA GATGATCCTC 
TCCAGTGAGA AGGGCCGGCT TCCTGAGCGT CTCACCAAGT TCCTTATCAC GCAGATCCTG 
GTGGCGCTGA GACACCTGCA CTTCAAGAAC ATCGTCCACT GTGACTTGAA ACCAGAGAAT 
GTGTTACTGG CATCAGCCGA TCCGTTTCCT CAGGTGAAGC TCTGTGACTT TGGCTTCGCT 
CGCATCATCG GCGAGAAGTC CTTCCGGCGC TCAGTGGTGG GCACGCCTGC CTACCTGGCA 
CCCGAGGTAC TGCTCAACCA GGGTTACAAC CGCTCGCTGG ACATGTGGTC CGTGGGTGTG 
ATCATGTACG TGAGCCTTAG TGGCACGTTC CCCTTCAATG AGGACGAGGA CATCAATGAC 
CAGATACAGA ACGCGGCCTT TATGTACCCG GCCAGCCCCT GGAGCCACAT CTCATCTGGA 
GCCATCGACC TCATCAACAA CCTGTTGCAG GTGAAGATGC GCAAGCGCTA CAGCGTGGAC 
AAGTCTCTCA GCCACCCATG GTTACAGGAG TACCAGACGT GGCTTGACCT CCGAGAGCTA 
GAGGGAAAGA TGGGCGAGCG ATATATCACG CACGAGAGCG ACGACGCACG CTGGGATCAG 
TTTGTGGCAG AGCGCCATGG GACTCCTGCA GAAGGGGACC TGGGTGGCGC CTGTCTGCCG 
CAGGACCACG AGATGCAGGG GCTGGCTGAG CGCATCAGCA TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849231.1
CDS	560..3187
Translation	MAAAPSHPAGLPGSPGPGSPPPPGGLDLQSPPPLLPQIPAPGSGVSFHIQIGLTREFVLLPAASELAHVKQLACSIVDQKFPECGFYGLYDKILLFKHDPTSANLLQLVRSAADIQEGDLVEVVLSASATFEDFQIRPHALTVHSYRAPAFCDHCGEMLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTSLASGHSVRLGSSESLPCTAEELSRSTTDLLPRRPPSSSSSSSSSSFYTGRPIELDKMLMSKVKVPHTFLIHSYTRPTVCQACKKLLKGLFRQGLQCKDCKFNCHKRCATRVPNDCLGEALINGDVPMEEAADYSEADKSSISDELEDSGVIPGSHSESALHASEEEEGEGHKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILAVEPAQNFSLVPPGTNPHCFEIITANVTYFVGETPGGAPGGPSGQGTEAVRGWETAIRQALMPVILQDAPSAPGHTPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYPASPWSHISSGAIDLINNLLQVKMRKRYSVDKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWDQFVAERHGTPAEGDLGGACLPQDHEMQGLAERISIL

Target ORF information:

RefSeq Version	NM_178900.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase D2 (Prkd2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178900.4

ORF Insert Sequence:

ATGGCCGCCG CCCCCTCCCA TCCCGCAGGA CTCCCCGGCT CTCCGGGGCC CGGGTCTCCT 
CCGCCCCCCG GTGGCTTGGA TCTCCAGTCG CCGCCACCTC TGCTGCCTCA GATCCCGGCT 
CCGGGTTCGG GGGTCTCCTT CCATATCCAG ATCGGATTGA CCCGAGAGTT CGTGCTGTTG 
CCGGCTGCCT CAGAGTTGGC TCATGTGAAG CAACTAGCCT GTTCTATCGT GGACCAGAAG 
TTCCCAGAGT GTGGCTTCTA CGGCCTGTAC GACAAGATCC TGCTCTTCAA ACATGACCCA 
ACATCAGCCA ACCTCCTGCA GCTGGTGCGA TCAGCTGCAG ATATCCAGGA GGGTGACCTG 
GTGGAGGTGG TTCTGTCGGC CTCGGCCACC TTCGAGGATT TCCAGATCCG ACCGCATGCT 
CTCACTGTGC ACTCGTACCG CGCTCCCGCC TTCTGTGACC ACTGCGGGGA GATGCTCTTC 
GGCCTCGTGC GCCAGGGCCT CAAGTGCGAT GGCTGCGGGC TGAATTACCA CAAACGCTGC 
GCCTTCAGCA TCCCGAACAA CTGCAGTGGT GCTCGAAAGC GCCGCCTGTC ATCCACGTCT 
CTGGCCAGTG GCCATTCTGT GCGCCTTGGC AGCTCCGAGT CCTTACCCTG CACCGCCGAA 
GAGCTGAGCC GTAGCACCAC CGACCTCCTT CCTCGCCGAC CACCCTCGTC GTCGTCCTCC 
TCCTCTTCCT CTTCCTTCTA CACAGGCCGA CCCATTGAGT TGGACAAGAT GCTGATGTCC 
AAGGTCAAGG TGCCACACAC CTTCCTTATA CATAGCTACA CACGGCCCAC CGTTTGCCAG 
GCTTGCAAGA AACTGCTCAA GGGTCTATTC CGCCAGGGCC TGCAGTGCAA AGACTGCAAG 
TTTAACTGTC ACAAACGCTG TGCTACCCGC GTCCCTAACG ACTGCCTGGG GGAGGCGCTC 
ATCAATGGAG ACGTGCCGAT GGAGGAAGCC GCTGATTACA GTGAGGCTGA TAAGAGCTCC 
ATCTCAGATG AGTTGGAGGA TTCTGGTGTC ATCCCCGGCT CCCACTCAGA GAGTGCTCTC 
CATGCCAGTG AGGAAGAGGA AGGCGAGGGA CACAAGGCCC AGAGCTCGCT GGGATACATC 
CCCCTGATGC GCGTGGTACA GTCTGTGCGA CACACGACCC GGAAATCCAG CACCACCCTG 
CGGGAGGGCT GGGTGGTCCA TTACAGCAAC AAGGACACAC TGAGGAAACG GCACTACTGG 
CGTCTAGACT GTAAATGCAT TACCCTCTTC CAGAACAACA CGACCAACAG ATACTACAAG 
GAAATCCCGC TGTCTGAAAT CCTTGCGGTG GAACCGGCTC AGAACTTCAG CCTCGTACCC 
CCGGGCACCA ACCCACACTG CTTCGAGATC ATCACGGCCA ATGTCACCTA CTTTGTGGGC 
GAAACCCCTG GCGGGGCCCC GGGAGGGCCA AGTGGACAGG GAACAGAGGC CGTTCGGGGT 
TGGGAGACAG CCATCCGCCA GGCACTGATG CCCGTTATCC TCCAAGATGC GCCCAGTGCC 
CCGGGGCACA CACCGCACAG ACAAGCGTCT CTGAGTATCT CCGTGTCCAA CAGTCAGATC 
CAAGAGAATG TGGATATCGC CACTGTCTAC CAGATCTTCC CAGATGAGGT GCTGGGCTCT 
GGGCAGTTTG GAGTGGTTTA TGGAGGAAAA CACAGGAAGA CTGGCAGAGA CGTTGCAGTA 
AAGGTCATTG ACAAACTGCG CTTCCCCACC AAGCAGGAGA GCCAGCTCAG GAATGAAGTG 
GCCATTCTCC AGAGTCTACG GCACCCCGGG ATTGTGAACC TCGAGTGCAT GTTTGAGACT 
CCTGAGAAGG TATTCGTGGT GATGGAGAAA CTGCACGGGG ACATGTTGGA GATGATCCTC 
TCCAGTGAGA AGGGCCGGCT TCCTGAGCGT CTCACCAAGT TCCTTATCAC GCAGATCCTG 
GTGGCGCTGA GACACCTGCA CTTCAAGAAC ATCGTCCACT GTGACTTGAA ACCAGAGAAT 
GTGTTACTGG CATCAGCCGA TCCGTTTCCT CAGGTGAAGC TCTGTGACTT TGGCTTCGCT 
CGCATCATCG GCGAGAAGTC CTTCCGGCGC TCAGTGGTGG GCACGCCTGC CTACCTGGCA 
CCCGAGGTAC TGCTCAACCA GGGTTACAAC CGCTCGCTGG ACATGTGGTC CGTGGGTGTG 
ATCATGTACG TGAGCCTTAG TGGCACGTTC CCCTTCAATG AGGACGAGGA CATCAATGAC 
CAGATACAGA ACGCGGCCTT TATGTACCCG GCCAGCCCCT GGAGCCACAT CTCATCTGGA 
GCCATCGACC TCATCAACAA CCTGTTGCAG GTGAAGATGC GCAAGCGCTA CAGCGTGGAC 
AAGTCTCTCA GCCACCCATG GTTACAGGAG TACCAGACGT GGCTTGACCT CCGAGAGCTA 
GAGGGAAAGA TGGGCGAGCG ATATATCACG CACGAGAGCG ACGACGCACG CTGGGATCAG 
TTTGTGGCAG AGCGCCATGG GACTCCTGCA GAAGGGGACC TGGGTGGCGC CTGTCTGCCG 
CAGGACCACG AGATGCAGGG GCTGGCTGAG CGCATCAGCA TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkd2 ( XM_006539444.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539507.1 Mus musculus protein kinase D2 (Prkd2), transcript variant X2, mRNA.

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CloneID	OMu37943
Clone ID Related Accession (Same CDS sequence)	XM_006539444.1 , XM_006539444.2
Accession Version	XM_006539444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase D2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539444.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAG CCGCTGATTA CAGTGAGGCT GATAAGAGCT CCATCTCAGA TGAGTTGGAG 
GATTCTGGTG TCATCCCCGG CTCCCACTCA GAGAGTGCTC TCCATGCCAG TGAGGAAGAG 
GAAGGCGAGG GACACAAGGC CCAGAGCTCG CTGGGATACA TCCCCCTGAT GCGCGTGGTA 
CAGTCTGTGC GACACACGAC CCGGAAATCC AGCACCACCC TGCGGGAGGG CTGGGTGGTC 
CATTACAGCA ACAAGGACAC ACTGAGGAAA CGGCACTACT GGCGTCTAGA CTGTAAATGC 
ATTACCCTCT TCCAGAACAA CACGACCAAC AGATACTACA AGGAAATCCC GCTGTCTGAA 
ATCCTTGCGG TGGAACCGGC TCAGAACTTC AGCCTCGTAC CCCCGGGCAC CAACCCACAC 
TGCTTCGAGA TCATCACGGC CAATGTCACC TACTTTGTGG GCGAAACCCC TGGCGGGGCC 
CCGGGAGGGC CAAGTGGACA GGGAACAGAG GCCGTTCGGG GTTGGGAGAC AGCCATCCGC 
CAGGCACTGA TGCCCGTTAT CCTCCAAGAT GCGCCCAGTG CCCCGGGGCA CACACCGCAC 
AGACAAGCGT CTCTGAGTAT CTCCGTGTCC AACAGTCAGA TCCAAGAGAA TGTGGATATC 
GCCACTGTCT ACCAGATCTT CCCAGATGAG GTGCTGGGCT CTGGGCAGTT TGGAGTGGTT 
TATGGAGGAA AACACAGGAA GACTGGCAGA GACGTTGCAG TAAAGGTCAT TGACAAACTG 
CGCTTCCCCA CCAAGCAGGA GAGCCAGCTC AGGAATGAAG TGGCCATTCT CCAGAGTCTA 
CGGCACCCCG GGATTGTGAA CCTCGAGTGC ATGTTTGAGA CTCCTGAGAA GGTATTCGTG 
GTGATGGAGA AACTGCACGG GGACATGTTG GAGATGATCC TCTCCAGTGA GAAGGGCCGG 
CTTCCTGAGC GTCTCACCAA GTTCCTTATC ACGCAGATCC TGGTGGCGCT GAGACACCTG 
CACTTCAAGA ACATCGTCCA CTGTGACTTG AAACCAGAGA ATGTGTTACT GGCATCAGCC 
GATCCGTTTC CTCAGGTGAA GCTCTGTGAC TTTGGCTTCG CTCGCATCAT CGGCGAGAAG 
TCCTTCCGGC GCTCAGTGGT GGGCACGCCT GCCTACCTGG CACCCGAGGT ACTGCTCAAC 
CAGGGTTACA ACCGCTCGCT GGACATGTGG TCCGTGGGTG TGATCATGTA CGTGAGCCTT 
AGTGGCACGT TCCCCTTCAA TGAGGACGAG GACATCAATG ACCAGATACA GAACGCGGCC 
TTTATGTACC CGGCCAGCCC CTGGAGCCAC ATCTCATCTG GAGCCATCGA CCTCATCAAC 
AACCTGTTGC AGGTGAAGAT GCGCAAGCGC TACAGCGTGG ACAAGTCTCT CAGCCACCCA 
TGGTTACAGG AGTACCAGAC GTGGCTTGAC CTCCGAGAGC TAGAGGGAAA GATGGGCGAG 
CGATATATCA CGCACGAGAG CGACGACGCA CGCTGGGATC AGTTTGTGGC AGAGCGCCAT 
GGGACTCCTG CAGAAGGGGA CCTGGGTGGC GCCTGTCTGC CGCAGGACCA CGAGATGCAG 
GGGCTGGCTG AGCGCATCAG CATCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539507.1
CDS	1244..2893
Translation	MEEAADYSEADKSSISDELEDSGVIPGSHSESALHASEEEEGEGHKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILAVEPAQNFSLVPPGTNPHCFEIITANVTYFVGETPGGAPGGPSGQGTEAVRGWETAIRQALMPVILQDAPSAPGHTPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYPASPWSHISSGAIDLINNLLQVKMRKRYSVDKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWDQFVAERHGTPAEGDLGGACLPQDHEMQGLAERISIL

Target ORF information:

RefSeq Version	XM_006539444.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase D2 (Prkd2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539444.2

ORF Insert Sequence:

ATGGAGGAAG CCGCTGATTA CAGTGAGGCT GATAAGAGCT CCATCTCAGA TGAGTTGGAG 
GATTCTGGTG TCATCCCCGG CTCCCACTCA GAGAGTGCTC TCCATGCCAG TGAGGAAGAG 
GAAGGCGAGG GACACAAGGC CCAGAGCTCG CTGGGATACA TCCCCCTGAT GCGCGTGGTA 
CAGTCTGTGC GACACACGAC CCGGAAATCC AGCACCACCC TGCGGGAGGG CTGGGTGGTC 
CATTACAGCA ACAAGGACAC ACTGAGGAAA CGGCACTACT GGCGTCTAGA CTGTAAATGC 
ATTACCCTCT TCCAGAACAA CACGACCAAC AGATACTACA AGGAAATCCC GCTGTCTGAA 
ATCCTTGCGG TGGAACCGGC TCAGAACTTC AGCCTCGTAC CCCCGGGCAC CAACCCACAC 
TGCTTCGAGA TCATCACGGC CAATGTCACC TACTTTGTGG GCGAAACCCC TGGCGGGGCC 
CCGGGAGGGC CAAGTGGACA GGGAACAGAG GCCGTTCGGG GTTGGGAGAC AGCCATCCGC 
CAGGCACTGA TGCCCGTTAT CCTCCAAGAT GCGCCCAGTG CCCCGGGGCA CACACCGCAC 
AGACAAGCGT CTCTGAGTAT CTCCGTGTCC AACAGTCAGA TCCAAGAGAA TGTGGATATC 
GCCACTGTCT ACCAGATCTT CCCAGATGAG GTGCTGGGCT CTGGGCAGTT TGGAGTGGTT 
TATGGAGGAA AACACAGGAA GACTGGCAGA GACGTTGCAG TAAAGGTCAT TGACAAACTG 
CGCTTCCCCA CCAAGCAGGA GAGCCAGCTC AGGAATGAAG TGGCCATTCT CCAGAGTCTA 
CGGCACCCCG GGATTGTGAA CCTCGAGTGC ATGTTTGAGA CTCCTGAGAA GGTATTCGTG 
GTGATGGAGA AACTGCACGG GGACATGTTG GAGATGATCC TCTCCAGTGA GAAGGGCCGG 
CTTCCTGAGC GTCTCACCAA GTTCCTTATC ACGCAGATCC TGGTGGCGCT GAGACACCTG 
CACTTCAAGA ACATCGTCCA CTGTGACTTG AAACCAGAGA ATGTGTTACT GGCATCAGCC 
GATCCGTTTC CTCAGGTGAA GCTCTGTGAC TTTGGCTTCG CTCGCATCAT CGGCGAGAAG 
TCCTTCCGGC GCTCAGTGGT GGGCACGCCT GCCTACCTGG CACCCGAGGT ACTGCTCAAC 
CAGGGTTACA ACCGCTCGCT GGACATGTGG TCCGTGGGTG TGATCATGTA CGTGAGCCTT 
AGTGGCACGT TCCCCTTCAA TGAGGACGAG GACATCAATG ACCAGATACA GAACGCGGCC 
TTTATGTACC CGGCCAGCCC CTGGAGCCAC ATCTCATCTG GAGCCATCGA CCTCATCAAC 
AACCTGTTGC AGGTGAAGAT GCGCAAGCGC TACAGCGTGG ACAAGTCTCT CAGCCACCCA 
TGGTTACAGG AGTACCAGAC GTGGCTTGAC CTCCGAGAGC TAGAGGGAAA GATGGGCGAG 
CGATATATCA CGCACGAGAG CGACGACGCA CGCTGGGATC AGTTTGTGGC AGAGCGCCAT 
GGGACTCCTG CAGAAGGGGA CCTGGGTGGC GCCTGTCTGC CGCAGGACCA CGAGATGCAG 
GGGCTGGCTG AGCGCATCAG CATCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein kinase D regulates positive selection of CD4
Nature communications712756(2016 Sep)
Ishikawa E,Kosako H,Yasuda T,Ohmuraya M,Araki K,Kurosaki T,Saito T,Yamasaki S

Protein kinase D1/2 is involved in the maturation of multivesicular bodies and secretion of exosomes in T and B lymphocytes.
Cell death and differentiation23(1)99-109(2016 Jan)
Mazzeo C,Calvo V,Alonso R,M?rida I,Izquierdo M

Functional redundancy of protein kinase D1 and protein kinase D2 in neuronal polarity.
Neuroscience research9512-20(2015 Jun)
Avriyanti E,Atik N,Kunii M,Furumoto N,Iwano T,Yoshimura S,Harada R,Harada A

Protein kinase D2 is a digital amplifier of T cell receptor-stimulated diacylglycerol signaling in na?ve CD8? T cells.
Science signaling7(348)ra99(2014 Oct)
Navarro MN,Feijoo-Carnero C,Arandilla AG,Trost M,Cantrell DA

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Prkd2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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