ESYT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ESYT1
Entrez Gene ID	23344
Full Name	extended synaptotagmin 1
Synonyms	FAM62A,MBC2
General protein information	Preferred Names extended synaptotagmin 1 Names extended synaptotagmin-1 extended synaptotagmin like protein 1 extended synaptotagmin protein 1 family with sequence similarity 62 (C2 domain containing), member A membrane-bound C2 domain-containing protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.2
Disorder MIM:	616670

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184796.1	NP_001171725.1	extended synaptotagmin-1 isoform 1
NM_015292.2	NP_056107.1	extended synaptotagmin-1 isoform 2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	esyt1a	XP_695611.3
Arabidopsis thaliana (thale cress)	SYTA	NP_565495.1
Homo sapiens (human)	ESYT1	NP_001171725.1
Pan troglodytes (chimpanzee)	ESYT1	XP_509135.2
	ESYT1	XP_001114269.1
Rattus norvegicus (Norway rat)	Esyt1	NP_058945.2
Danio rerio (zebrafish)	esyt1b	XP_699731.6
Oryza sativa (rice)	Os02g0448400	NP_001046755.1
Oryza sativa (rice)	Os09g0538800	NP_001063800.1
Xenopus tropicalis (tropical clawed frog)	esyt1	XP_002934579.1
Canis lupus familiaris (dog)	ESYT1	XP_005625706.1
Bos taurus (cattle)	ESYT1	NP_001071411.1
Mus musculus (house mouse)	Esyt1	NP_035973.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel microscopy-based assay identifies extended synaptotagmin-1 (ESYT1) as a positive regulator of anoctamin 1 traffic.
L?rias JR, Pinto MC, Botelho HM, Awatade NT, Quaresma MC, Silva IAL, Wanitchakool P, Schreiber R, Pepperkok R, Kunzelmann K, Amaral MD
Biochimica et biophysica acta1865(2)421-431(2018 Feb)

Ca
Bian X, Saheki Y, De Camilli P
The EMBO journal37(2)219-234(2018 Jan)

Control of plasma membrane lipid homeostasis by the extended synaptotagmins.
Saheki Y, Bian X, Schauder CM, Sawaki Y, Surma MA, Klose C, Pincet F, Reinisch KM, De Camilli P
Nature cell biology18(5)504-15(2016 05)

Extended Synaptotagmin Interaction with the Fibroblast Growth Factor Receptor Depends on Receptor Conformation, Not Catalytic Activity.
Tremblay MG, Herdman C, Guillou F, Mishra PK, Baril J, Bellenfant S, Moss T
The Journal of biological chemistry290(26)16142-56(2015 Jun)

Proteomic and genomic evidence implicates the postsynaptic density in schizophrenia.
F?cking M, Lopez LM, English JA, Dicker P, Wolff A, Brindley E, Wynne K, Cagney G, Cotter DR
Molecular psychiatry20(4)424-32(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ESyt2 and ESyt3, but not ESyt1, interact with activated FGFR1.
Title: Extended Synaptotagmin Interaction with the Fibroblast Growth Factor Receptor Depends on Receptor Conformation, Not Catalytic Activity.

This study identified and confirmed ESYT1 protein changes within the postsynaptic density in schizophrenia.
Title: Proteomic and genomic evidence implicates the postsynaptic density in schizophrenia.

The extended synaptotagmins (E-Syt1, E-Syt2, and E-Syt3) are endoplasmic reticulum-anchored proteins. They form homo- and heteromeric complexes that mediate contacts with the plasma membrane. Such contacts are critically dependent on the presence of PI(4,5)P2 in this membrane and are additionally regulated by cytosolic Ca2+ via the Ca2+-sensing property of E-Syt1.
Title: PI(4,5)P(2)-dependent and Ca(2+)-regulated ER-PM interactions mediated by the extended synaptotagmins.

E-Syt1 is a mediator of cancer cell invasion.
Title: The oncogenic lung cancer fusion kinase CD74-ROS activates a novel invasiveness pathway through E-Syt1 phosphorylation.

The following ESYT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESYT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu55597	NM_001184796.1 Latest version!	Homo sapiens extended synaptotagmin 1 (ESYT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu55658	NM_015292.2 Latest version!	Homo sapiens extended synaptotagmin 1 (ESYT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ESYT1 ( NM_001184796.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171725.1 Homo sapiens extended synaptotagmin 1 (ESYT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55597
Clone ID Related Accession (Same CDS sequence)	NM_001184796.1
Accession Version	NM_001184796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	extended synaptotagmin-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB100366.1, AB018290.1 and AJ346011.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.118586.1, SRR1660809.138444.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGAT CTCCAGGAGA GGGCCCCAGC CCCAGCCCCA TGGACCAGCC CTCTGCTCCC 
TCCGACCCCA CTGACCAGCC CCCCGCTGCT CACGCAAAGC CAGACCCAGG TTCTGGGGGC 
CAACCTGCTG GCCCTGGCGC GGCGGGTGAG GCCCTGGCGG TGCTGACTTC ATTCGGGAGG 
CGGTTGCTGG TGCTGATACC TGTGTATTTG GCCGGGGCAG TGGGACTCAG CGTGGGTTTC 
GTGCTCTTCG GCCTCGCCCT CTACCTGGGC TGGCGCCGGG TCCGCGACGA GAAAGAACGG 
AGCCTTCGAG CAGCGAGGCA GCTACTGGAC GACGAGGAGC AGCTCACTGC GAAAACTCTC 
TATATGAGTC ATCGAGAGCT ACCTGCCTGG GTCAGCTTCC CAGACGTGGA AAAGGCTGAA 
TGGCTCAATA AGATTGTGGC CCAGGTCTGG CCCTTCCTGG GCCAGTATAT GGAGAAGCTT 
CTGGCTGAAA CTGTGGCTCC GGCTGTTAGG GGATCTAACC CCCATCTGCA AACATTTACA 
TTTACACGAG TGGAACTGGG TGAAAAGCCA TTGCGCATCA TTGGAGTCAA GGTTCACCCA 
GGTCAGAGAA AAGAGCAGAT CCTGCTGGAC TTGAACATCA GCTATGTAGG TGATGTGCAG 
ATTGATGTGG AAGTGAAGAA ATATTTTTGC AAAGCAGGAG TCAAGGGCAT GCAGCTACAT 
GGCGTTTTGC GGGTGATACT GGAGCCACTC ATTGGGGACC TTCCCTTCGT GGGGGCTGTG 
TCAATGTTCT TCATCCGACG CCCGACCCTA GACATCAACT GGACAGGGAT GACCAACCTG 
CTGGATATCC CAGGACTTAG CTCACTCTCT GACACCATGA TCATGGACTC CATTGCTGCC 
TTCCTCGTGT TGCCCAACCG ATTACTGGTG CCCCTTGTGC CTGACCTTCA AGATGTGGCT 
CAGTTGCGTT CCCCTCTGCC CAGGGGCATT ATTCGAATTC ACCTGCTGGC TGCTCGAGGG 
CTGAGTTCCA AGGACAAATA TGTGAAGGGC CTGATTGAGG GCAAGTCAGA CCCATATGCA 
CTTGTGCGTT TGGGTACCCA GACATTCTGC AGTCGTGTCA TTGATGAAGA ACTCAACCCA 
CAGTGGGGAG AGACTTATGA GGTGATGGTA CACGAGGTCC CAGGGCAGGA GATTGAAGTG 
GAGGTGTTCG ACAAGGATCC AGATAAAGAT GACTTTCTGG GCAGAATGAA GCTGGATGTA 
GGGAAGGTGT TACAGGCTAG CGTTCTGGAT GATTGGTTCC CTCTACAAGG TGGGCAAGGC 
CAAGTTCACT TGAGGCTAGA ATGGCTGTCA CTTTTGTCAG ATGCAGAGAA ACTGGAGCAG 
GTTCTACAGT GGAATTGGGG AGTCTCCTCT CGACCAGATC CCCCGTCAGC TGCCATCTTA 
GTTGTCTACC TGGATCGGGC CCAGGATCTT CCTATGGTGA CCTCTGAATT GTACCCACCA 
CAGCTGAAGA AGGGGAACAA GGAACCCAAC CCTATGGTAC AACTGTCAAT TCAGGATGTG 
ACTCAGGAGA GCAAGGCTGT CTACAGTACC AACTGCCCAG TGTGGGAGGA AGCGTTCCGG 
TTCTTCCTAC AAGACCCTCA AAGCCAGGAG CTCGATGTGC AAGTGAAGGA TGATTCCAGG 
GCCCTGACTT TAGGAGCACT GACGCTGCCT CTGGCCCGCC TGCTGACTGC CCCAGAACTC 
ATCCTGGACC AGTGGTTCCA GCTCAGCAGC TCTGGTCCAA ACTCCAGACT CTATATGAAA 
CTAGTCATGA GGATCCTGTA CTTGGATTCA TCAGAAATAT GCTTCCCCAC GGTGCCTGGT 
TGTCCTGGTG CTTGGGACGT GGACAGTGAG AATCCCCAGA GAGGCAGCAG TGTGGATGCC 
CCACCTCGAC CCTGTCACAC GACTCCTGAT AGCCAGTTTG GGACTGAGCA TGTGCTTCGG 
ATCCATGTAT TAGAGGCCCA GGACCTGATT GCCAAAGACC GTTTCTTGGG GGGACTGGTG 
AAGGGCAAGT CAGACCCCTA TGTCAAACTA AAGTTGGCAG GACGAAGCTT CCGGAGCCAT 
GTTGTTCGGG AAGATCTCAA TCCCCGCTGG AATGAGGTTT TTGAGGTGAT CGTCACATCA 
GTTCCAGGCC AAGAGCTAGA GGTTGAAGTC TTTGACAAGG ACTTGGACAA GGATGATTTT 
CTGGGCAGGT GTAAAGTGCG TCTCACCACA GTCTTAAACA GTGGCTTCCT TGATGAGTGG 
CTGACCCTGG AGGATGTCCC ATCTGGCCGC CTGCACTTGC GCCTGGAGCG TCTCACCCCC 
CGTCCCACTG CTGCTGAGTT AGAGGAGGTG CTGCAGGTGA ATAGTTTGAT CCAGACTCAG 
AAGAGTGCGG AGCTGGCTGC GGCCCTGCTA TCCATCTATA TGGAGCGGGC AGAGGACCTC 
CCGCTGCGAA AAGGCACCAA GCACCTCAGC CCTTATGCTA CTCTCACTGT GGGAGATAGT 
TCTCATAAAA CCAAGACTAT TTCGCAAACT TCAGCCCCTG TCTGGGATGA GAGTGCCTCC 
TTTCTCATCA GGAAACCACA CACTGAGAGC CTAGAGTTGC AGGTTCGGGG TGAGGGCACT 
GGCGTGCTGG GCTCATTATC CCTGCCCCTC TCAGAGCTCC TCGTGGCTGA CCAGCTCTGC 
TTGGACCGCT GGTTTACACT CAGCAGTGGT CAGGGGCAGG TGCTACTGAG AGCACAGCTA 
GGGATCCTGG TGTCCCAGCA CTCGGGAGTG GAAGCTCATA GCCACAGCTA CAGCCACAGC 
TCCTCATCGC TGAGTGAAGA ACCAGAGCTC TCGGGGGGAC CCCCTCACAT CACCTCCTCA 
GCCCCAGAGC TCCGGCAGCG CCTAACACAT GTTGACAGTC CCCTTGAGGC TCCAGCCGGG 
CCTCTGGGCC AGGTGAAACT GACTCTGTGG TACTACAGTG AAGAACGAAA GCTGGTCAGC 
ATTGTTCATG GTTGCCGGTC CCTTCGACAG AATGGACGTG ATCCTCCTGA TCCCTATGTG 
TCACTGTTGC TACTGCCAGA CAAGAACCGA GGCACCAAGA GGAGGACCTC ACAGAAGAAG 
AGGACCCTGA GTCCTGAATT TAATGAACGG TTTGAGTGGG AACTCCCCCT GGATGAGGCC 
CAGAGACGAA AGCTGGATGT CTCTGTCAAG TCTAATTCCT CCTTCATGTC AAGAGAGCGT 
GAGCTGCTGG GGAAGGTGCA GCTGGACCTA GCTGAGACAG ACCTTTCCCA GGGTGTAGCC 
CGGTGGTATG ACCTGATGGA CAACAAGGAC AAGGGCAGCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171725.1
CDS	119..3463
Translation	MERSPGEGPSPSPMDQPSAPSDPTDQPPAAHAKPDPGSGGQPAGPGAAGEALAVLTSFGRRLLVLIPVYLAGAVGLSVGFVLFGLALYLGWRRVRDEKERSLRAARQLLDDEEQLTAKTLYMSHRELPAWVSFPDVEKAEWLNKIVAQVWPFLGQYMEKLLAETVAPAVRGSNPHLQTFTFTRVELGEKPLRIIGVKVHPGQRKEQILLDLNISYVGDVQIDVEVKKYFCKAGVKGMQLHGVLRVILEPLIGDLPFVGAVSMFFIRRPTLDINWTGMTNLLDIPGLSSLSDTMIMDSIAAFLVLPNRLLVPLVPDLQDVAQLRSPLPRGIIRIHLLAARGLSSKDKYVKGLIEGKSDPYALVRLGTQTFCSRVIDEELNPQWGETYEVMVHEVPGQEIEVEVFDKDPDKDDFLGRMKLDVGKVLQASVLDDWFPLQGGQGQVHLRLEWLSLLSDAEKLEQVLQWNWGVSSRPDPPSAAILVVYLDRAQDLPMVTSELYPPQLKKGNKEPNPMVQLSIQDVTQESKAVYSTNCPVWEEAFRFFLQDPQSQELDVQVKDDSRALTLGALTLPLARLLTAPELILDQWFQLSSSGPNSRLYMKLVMRILYLDSSEICFPTVPGCPGAWDVDSENPQRGSSVDAPPRPCHTTPDSQFGTEHVLRIHVLEAQDLIAKDRFLGGLVKGKSDPYVKLKLAGRSFRSHVVREDLNPRWNEVFEVIVTSVPGQELEVEVFDKDLDKDDFLGRCKVRLTTVLNSGFLDEWLTLEDVPSGRLHLRLERLTPRPTAAELEEVLQVNSLIQTQKSAELAAALLSIYMERAEDLPLRKGTKHLSPYATLTVGDSSHKTKTISQTSAPVWDESASFLIRKPHTESLELQVRGEGTGVLGSLSLPLSELLVADQLCLDRWFTLSSGQGQVLLRAQLGILVSQHSGVEAHSHSYSHSSSSLSEEPELSGGPPHITSSAPELRQRLTHVDSPLEAPAGPLGQVKLTLWYYSEERKLVSIVHGCRSLRQNGRDPPDPYVSLLLLPDKNRGTKRRTSQKKRTLSPEFNERFEWELPLDEAQRRKLDVSVKSNSSFMSRERELLGKVQLDLAETDLSQGVARWYDLMDNKDKGSS

Target ORF information:

RefSeq Version	NM_001184796.1
Organism	Homo sapiens(human)
Definition	Homo sapiens extended synaptotagmin 1 (ESYT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184796.1

ORF Insert Sequence:

ATGGAGCGAT CTCCAGGAGA GGGCCCCAGC CCCAGCCCCA TGGACCAGCC CTCTGCTCCC 
TCCGACCCCA CTGACCAGCC CCCCGCTGCT CACGCAAAGC CAGACCCAGG TTCTGGGGGC 
CAACCTGCTG GCCCTGGCGC GGCGGGTGAG GCCCTGGCGG TGCTGACTTC ATTCGGGAGG 
CGGTTGCTGG TGCTGATACC TGTGTATTTG GCCGGGGCAG TGGGACTCAG CGTGGGTTTC 
GTGCTCTTCG GCCTCGCCCT CTACCTGGGC TGGCGCCGGG TCCGCGACGA GAAAGAACGG 
AGCCTTCGAG CAGCGAGGCA GCTACTGGAC GACGAGGAGC AGCTCACTGC GAAAACTCTC 
TATATGAGTC ATCGAGAGCT ACCTGCCTGG GTCAGCTTCC CAGACGTGGA AAAGGCTGAA 
TGGCTCAATA AGATTGTGGC CCAGGTCTGG CCCTTCCTGG GCCAGTATAT GGAGAAGCTT 
CTGGCTGAAA CTGTGGCTCC GGCTGTTAGG GGATCTAACC CCCATCTGCA AACATTTACA 
TTTACACGAG TGGAACTGGG TGAAAAGCCA TTGCGCATCA TTGGAGTCAA GGTTCACCCA 
GGTCAGAGAA AAGAGCAGAT CCTGCTGGAC TTGAACATCA GCTATGTAGG TGATGTGCAG 
ATTGATGTGG AAGTGAAGAA ATATTTTTGC AAAGCAGGAG TCAAGGGCAT GCAGCTACAT 
GGCGTTTTGC GGGTGATACT GGAGCCACTC ATTGGGGACC TTCCCTTCGT GGGGGCTGTG 
TCAATGTTCT TCATCCGACG CCCGACCCTA GACATCAACT GGACAGGGAT GACCAACCTG 
CTGGATATCC CAGGACTTAG CTCACTCTCT GACACCATGA TCATGGACTC CATTGCTGCC 
TTCCTCGTGT TGCCCAACCG ATTACTGGTG CCCCTTGTGC CTGACCTTCA AGATGTGGCT 
CAGTTGCGTT CCCCTCTGCC CAGGGGCATT ATTCGAATTC ACCTGCTGGC TGCTCGAGGG 
CTGAGTTCCA AGGACAAATA TGTGAAGGGC CTGATTGAGG GCAAGTCAGA CCCATATGCA 
CTTGTGCGTT TGGGTACCCA GACATTCTGC AGTCGTGTCA TTGATGAAGA ACTCAACCCA 
CAGTGGGGAG AGACTTATGA GGTGATGGTA CACGAGGTCC CAGGGCAGGA GATTGAAGTG 
GAGGTGTTCG ACAAGGATCC AGATAAAGAT GACTTTCTGG GCAGAATGAA GCTGGATGTA 
GGGAAGGTGT TACAGGCTAG CGTTCTGGAT GATTGGTTCC CTCTACAAGG TGGGCAAGGC 
CAAGTTCACT TGAGGCTAGA ATGGCTGTCA CTTTTGTCAG ATGCAGAGAA ACTGGAGCAG 
GTTCTACAGT GGAATTGGGG AGTCTCCTCT CGACCAGATC CCCCGTCAGC TGCCATCTTA 
GTTGTCTACC TGGATCGGGC CCAGGATCTT CCTATGGTGA CCTCTGAATT GTACCCACCA 
CAGCTGAAGA AGGGGAACAA GGAACCCAAC CCTATGGTAC AACTGTCAAT TCAGGATGTG 
ACTCAGGAGA GCAAGGCTGT CTACAGTACC AACTGCCCAG TGTGGGAGGA AGCGTTCCGG 
TTCTTCCTAC AAGACCCTCA AAGCCAGGAG CTCGATGTGC AAGTGAAGGA TGATTCCAGG 
GCCCTGACTT TAGGAGCACT GACGCTGCCT CTGGCCCGCC TGCTGACTGC CCCAGAACTC 
ATCCTGGACC AGTGGTTCCA GCTCAGCAGC TCTGGTCCAA ACTCCAGACT CTATATGAAA 
CTAGTCATGA GGATCCTGTA CTTGGATTCA TCAGAAATAT GCTTCCCCAC GGTGCCTGGT 
TGTCCTGGTG CTTGGGACGT GGACAGTGAG AATCCCCAGA GAGGCAGCAG TGTGGATGCC 
CCACCTCGAC CCTGTCACAC GACTCCTGAT AGCCAGTTTG GGACTGAGCA TGTGCTTCGG 
ATCCATGTAT TAGAGGCCCA GGACCTGATT GCCAAAGACC GTTTCTTGGG GGGACTGGTG 
AAGGGCAAGT CAGACCCCTA TGTCAAACTA AAGTTGGCAG GACGAAGCTT CCGGAGCCAT 
GTTGTTCGGG AAGATCTCAA TCCCCGCTGG AATGAGGTTT TTGAGGTGAT CGTCACATCA 
GTTCCAGGCC AAGAGCTAGA GGTTGAAGTC TTTGACAAGG ACTTGGACAA GGATGATTTT 
CTGGGCAGGT GTAAAGTGCG TCTCACCACA GTCTTAAACA GTGGCTTCCT TGATGAGTGG 
CTGACCCTGG AGGATGTCCC ATCTGGCCGC CTGCACTTGC GCCTGGAGCG TCTCACCCCC 
CGTCCCACTG CTGCTGAGTT AGAGGAGGTG CTGCAGGTGA ATAGTTTGAT CCAGACTCAG 
AAGAGTGCGG AGCTGGCTGC GGCCCTGCTA TCCATCTATA TGGAGCGGGC AGAGGACCTC 
CCGCTGCGAA AAGGCACCAA GCACCTCAGC CCTTATGCTA CTCTCACTGT GGGAGATAGT 
TCTCATAAAA CCAAGACTAT TTCGCAAACT TCAGCCCCTG TCTGGGATGA GAGTGCCTCC 
TTTCTCATCA GGAAACCACA CACTGAGAGC CTAGAGTTGC AGGTTCGGGG TGAGGGCACT 
GGCGTGCTGG GCTCATTATC CCTGCCCCTC TCAGAGCTCC TCGTGGCTGA CCAGCTCTGC 
TTGGACCGCT GGTTTACACT CAGCAGTGGT CAGGGGCAGG TGCTACTGAG AGCACAGCTA 
GGGATCCTGG TGTCCCAGCA CTCGGGAGTG GAAGCTCATA GCCACAGCTA CAGCCACAGC 
TCCTCATCGC TGAGTGAAGA ACCAGAGCTC TCGGGGGGAC CCCCTCACAT CACCTCCTCA 
GCCCCAGAGC TCCGGCAGCG CCTAACACAT GTTGACAGTC CCCTTGAGGC TCCAGCCGGG 
CCTCTGGGCC AGGTGAAACT GACTCTGTGG TACTACAGTG AAGAACGAAA GCTGGTCAGC 
ATTGTTCATG GTTGCCGGTC CCTTCGACAG AATGGACGTG ATCCTCCTGA TCCCTATGTG 
TCACTGTTGC TACTGCCAGA CAAGAACCGA GGCACCAAGA GGAGGACCTC ACAGAAGAAG 
AGGACCCTGA GTCCTGAATT TAATGAACGG TTTGAGTGGG AACTCCCCCT GGATGAGGCC 
CAGAGACGAA AGCTGGATGT CTCTGTCAAG TCTAATTCCT CCTTCATGTC AAGAGAGCGT 
GAGCTGCTGG GGAAGGTGCA GCTGGACCTA GCTGAGACAG ACCTTTCCCA GGGTGTAGCC 
CGGTGGTATG ACCTGATGGA CAACAAGGAC AAGGGCAGCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESYT1 ( NM_015292.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_056107.1 Homo sapiens extended synaptotagmin 1 (ESYT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55658
Clone ID Related Accession (Same CDS sequence)	NM_015292.2
Accession Version	NM_015292.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	extended synaptotagmin-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB100366.1, BC004998.1 and AJ346011.1. On May 20, 2010 this sequence version replaced NM_015292.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.30799.1, SRR1660809.127345.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGAT CTCCAGGAGA GGGCCCCAGC CCCAGCCCCA TGGACCAGCC CTCTGCTCCC 
TCCGACCCCA CTGACCAGCC CCCCGCTGCT CACGCAAAGC CAGACCCAGG TTCTGGGGGC 
CAACCTGCTG GCCCTGGCGC GGCGGGTGAG GCCCTGGCGG TGCTGACTTC ATTCGGGAGG 
CGGTTGCTGG TGCTGATACC TGTGTATTTG GCCGGGGCAG TGGGACTCAG CGTGGGTTTC 
GTGCTCTTCG GCCTCGCCCT CTACCTGGGC TGGCGCCGGG TCCGCGACGA GAAAGAACGG 
AGCCTTCGAG CAGCGAGGCA GCTACTGGAC GACGAGGAGC AGCTCACTGC GAAAACTCTC 
TATATGAGTC ATCGAGAGCT ACCTGCCTGG GTCAGCTTCC CAGACGTGGA AAAGGCTGAA 
TGGCTCAATA AGATTGTGGC CCAGGTCTGG CCCTTCCTGG GCCAGTATAT GGAGAAGCTT 
CTGGCTGAAA CTGTGGCTCC GGCTGTTAGG GGATCTAACC CCCATCTGCA AACATTTACA 
TTTACACGAG TGGAACTGGG TGAAAAGCCA TTGCGCATCA TTGGAGTCAA GGTTCACCCA 
GGTCAGAGAA AAGAGCAGAT CCTGCTGGAC TTGAACATCA GCTATGTAGG TGATGTGCAG 
ATTGATGTGG AAGTGAAGAA ATATTTTTGC AAAGCAGGAG TCAAGGGCAT GCAGCTACAT 
GGCGTTTTGC GGGTGATACT GGAGCCACTC ATTGGGGACC TTCCCTTCGT GGGGGCTGTG 
TCAATGTTCT TCATCCGACG CCCGACCCTA GACATCAACT GGACAGGGAT GACCAACCTG 
CTGGATATCC CAGGACTTAG CTCACTCTCT GACACCATGA TCATGGACTC CATTGCTGCC 
TTCCTCGTGT TGCCCAACCG ATTACTGGTG CCCCTTGTGC CTGACCTTCA AGATGTGGCT 
CAGTTGCGTT CCCCTCTGCC CAGGGGCATT ATTCGAATTC ACCTGCTGGC TGCTCGAGGG 
CTGAGTTCCA AGGACAAATA TGTGAAGGGC CTGATTGAGG GCAAGTCAGA CCCATATGCA 
CTTGTGCGTT TGGGTACCCA GACATTCTGC AGTCGTGTCA TTGATGAAGA ACTCAACCCA 
CAGTGGGGAG AGACTTATGA GGTGATGGTA CACGAGGTCC CAGGGCAGGA GATTGAAGTG 
GAGGTGTTCG ACAAGGATCC AGATAAAGAT GACTTTCTGG GCAGAATGAA GCTGGATGTA 
GGGAAGGTGT TACAGGCTAG CGTTCTGGAT GATTGGTTCC CTCTACAAGG TGGGCAAGGC 
CAAGTTCACT TGAGGCTAGA ATGGCTGTCA CTTTTGTCAG ATGCAGAGAA ACTGGAGCAG 
GTTCTACAGT GGAATTGGGG AGTCTCCTCT CGACCAGATC CCCCGTCAGC TGCCATCTTA 
GTTGTCTACC TGGATCGGGC CCAGGATCTT CCTCTGAAGA AGGGGAACAA GGAACCCAAC 
CCTATGGTAC AACTGTCAAT TCAGGATGTG ACTCAGGAGA GCAAGGCTGT CTACAGTACC 
AACTGCCCAG TGTGGGAGGA AGCGTTCCGG TTCTTCCTAC AAGACCCTCA AAGCCAGGAG 
CTCGATGTGC AAGTGAAGGA TGATTCCAGG GCCCTGACTT TAGGAGCACT GACGCTGCCT 
CTGGCCCGCC TGCTGACTGC CCCAGAACTC ATCCTGGACC AGTGGTTCCA GCTCAGCAGC 
TCTGGTCCAA ACTCCAGACT CTATATGAAA CTAGTCATGA GGATCCTGTA CTTGGATTCA 
TCAGAAATAT GCTTCCCCAC GGTGCCTGGT TGTCCTGGTG CTTGGGACGT GGACAGTGAG 
AATCCCCAGA GAGGCAGCAG TGTGGATGCC CCACCTCGAC CCTGTCACAC GACTCCTGAT 
AGCCAGTTTG GGACTGAGCA TGTGCTTCGG ATCCATGTAT TAGAGGCCCA GGACCTGATT 
GCCAAAGACC GTTTCTTGGG GGGACTGGTG AAGGGCAAGT CAGACCCCTA TGTCAAACTA 
AAGTTGGCAG GACGAAGCTT CCGGAGCCAT GTTGTTCGGG AAGATCTCAA TCCCCGCTGG 
AATGAGGTTT TTGAGGTGAT CGTCACATCA GTTCCAGGCC AAGAGCTAGA GGTTGAAGTC 
TTTGACAAGG ACTTGGACAA GGATGATTTT CTGGGCAGGT GTAAAGTGCG TCTCACCACA 
GTCTTAAACA GTGGCTTCCT TGATGAGTGG CTGACCCTGG AGGATGTCCC ATCTGGCCGC 
CTGCACTTGC GCCTGGAGCG TCTCACCCCC CGTCCCACTG CTGCTGAGTT AGAGGAGGTG 
CTGCAGGTGA ATAGTTTGAT CCAGACTCAG AAGAGTGCGG AGCTGGCTGC GGCCCTGCTA 
TCCATCTATA TGGAGCGGGC AGAGGACCTC CCGCTGCGAA AAGGCACCAA GCACCTCAGC 
CCTTATGCTA CTCTCACTGT GGGAGATAGT TCTCATAAAA CCAAGACTAT TTCGCAAACT 
TCAGCCCCTG TCTGGGATGA GAGTGCCTCC TTTCTCATCA GGAAACCACA CACTGAGAGC 
CTAGAGTTGC AGGTTCGGGG TGAGGGCACT GGCGTGCTGG GCTCATTATC CCTGCCCCTC 
TCAGAGCTCC TCGTGGCTGA CCAGCTCTGC TTGGACCGCT GGTTTACACT CAGCAGTGGT 
CAGGGGCAGG TGCTACTGAG AGCACAGCTA GGGATCCTGG TGTCCCAGCA CTCGGGAGTG 
GAAGCTCATA GCCACAGCTA CAGCCACAGC TCCTCATCGC TGAGTGAAGA ACCAGAGCTC 
TCGGGGGGAC CCCCTCACAT CACCTCCTCA GCCCCAGAGC TCCGGCAGCG CCTAACACAT 
GTTGACAGTC CCCTTGAGGC TCCAGCCGGG CCTCTGGGCC AGGTGAAACT GACTCTGTGG 
TACTACAGTG AAGAACGAAA GCTGGTCAGC ATTGTTCATG GTTGCCGGTC CCTTCGACAG 
AATGGACGTG ATCCTCCTGA TCCCTATGTG TCACTGTTGC TACTGCCAGA CAAGAACCGA 
GGCACCAAGA GGAGGACCTC ACAGAAGAAG AGGACCCTGA GTCCTGAATT TAATGAACGG 
TTTGAGTGGG AACTCCCCCT GGATGAGGCC CAGAGACGAA AGCTGGATGT CTCTGTCAAG 
TCTAATTCCT CCTTCATGTC AAGAGAGCGT GAGCTGCTGG GGAAGGTGCA GCTGGACCTA 
GCTGAGACAG ACCTTTCCCA GGGTGTAGCC CGGTGGTATG ACCTGATGGA CAACAAGGAC 
AAGGGCAGCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056107.1
CDS	119..3433
Translation	MERSPGEGPSPSPMDQPSAPSDPTDQPPAAHAKPDPGSGGQPAGPGAAGEALAVLTSFGRRLLVLIPVYLAGAVGLSVGFVLFGLALYLGWRRVRDEKERSLRAARQLLDDEEQLTAKTLYMSHRELPAWVSFPDVEKAEWLNKIVAQVWPFLGQYMEKLLAETVAPAVRGSNPHLQTFTFTRVELGEKPLRIIGVKVHPGQRKEQILLDLNISYVGDVQIDVEVKKYFCKAGVKGMQLHGVLRVILEPLIGDLPFVGAVSMFFIRRPTLDINWTGMTNLLDIPGLSSLSDTMIMDSIAAFLVLPNRLLVPLVPDLQDVAQLRSPLPRGIIRIHLLAARGLSSKDKYVKGLIEGKSDPYALVRLGTQTFCSRVIDEELNPQWGETYEVMVHEVPGQEIEVEVFDKDPDKDDFLGRMKLDVGKVLQASVLDDWFPLQGGQGQVHLRLEWLSLLSDAEKLEQVLQWNWGVSSRPDPPSAAILVVYLDRAQDLPLKKGNKEPNPMVQLSIQDVTQESKAVYSTNCPVWEEAFRFFLQDPQSQELDVQVKDDSRALTLGALTLPLARLLTAPELILDQWFQLSSSGPNSRLYMKLVMRILYLDSSEICFPTVPGCPGAWDVDSENPQRGSSVDAPPRPCHTTPDSQFGTEHVLRIHVLEAQDLIAKDRFLGGLVKGKSDPYVKLKLAGRSFRSHVVREDLNPRWNEVFEVIVTSVPGQELEVEVFDKDLDKDDFLGRCKVRLTTVLNSGFLDEWLTLEDVPSGRLHLRLERLTPRPTAAELEEVLQVNSLIQTQKSAELAAALLSIYMERAEDLPLRKGTKHLSPYATLTVGDSSHKTKTISQTSAPVWDESASFLIRKPHTESLELQVRGEGTGVLGSLSLPLSELLVADQLCLDRWFTLSSGQGQVLLRAQLGILVSQHSGVEAHSHSYSHSSSSLSEEPELSGGPPHITSSAPELRQRLTHVDSPLEAPAGPLGQVKLTLWYYSEERKLVSIVHGCRSLRQNGRDPPDPYVSLLLLPDKNRGTKRRTSQKKRTLSPEFNERFEWELPLDEAQRRKLDVSVKSNSSFMSRERELLGKVQLDLAETDLSQGVARWYDLMDNKDKGSS

Target ORF information:

RefSeq Version	NM_015292.2
Organism	Homo sapiens(human)
Definition	Homo sapiens extended synaptotagmin 1 (ESYT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015292.2

ORF Insert Sequence:

ATGGAGCGAT CTCCAGGAGA GGGCCCCAGC CCCAGCCCCA TGGACCAGCC CTCTGCTCCC 
TCCGACCCCA CTGACCAGCC CCCCGCTGCT CACGCAAAGC CAGACCCAGG TTCTGGGGGC 
CAACCTGCTG GCCCTGGCGC GGCGGGTGAG GCCCTGGCGG TGCTGACTTC ATTCGGGAGG 
CGGTTGCTGG TGCTGATACC TGTGTATTTG GCCGGGGCAG TGGGACTCAG CGTGGGTTTC 
GTGCTCTTCG GCCTCGCCCT CTACCTGGGC TGGCGCCGGG TCCGCGACGA GAAAGAACGG 
AGCCTTCGAG CAGCGAGGCA GCTACTGGAC GACGAGGAGC AGCTCACTGC GAAAACTCTC 
TATATGAGTC ATCGAGAGCT ACCTGCCTGG GTCAGCTTCC CAGACGTGGA AAAGGCTGAA 
TGGCTCAATA AGATTGTGGC CCAGGTCTGG CCCTTCCTGG GCCAGTATAT GGAGAAGCTT 
CTGGCTGAAA CTGTGGCTCC GGCTGTTAGG GGATCTAACC CCCATCTGCA AACATTTACA 
TTTACACGAG TGGAACTGGG TGAAAAGCCA TTGCGCATCA TTGGAGTCAA GGTTCACCCA 
GGTCAGAGAA AAGAGCAGAT CCTGCTGGAC TTGAACATCA GCTATGTAGG TGATGTGCAG 
ATTGATGTGG AAGTGAAGAA ATATTTTTGC AAAGCAGGAG TCAAGGGCAT GCAGCTACAT 
GGCGTTTTGC GGGTGATACT GGAGCCACTC ATTGGGGACC TTCCCTTCGT GGGGGCTGTG 
TCAATGTTCT TCATCCGACG CCCGACCCTA GACATCAACT GGACAGGGAT GACCAACCTG 
CTGGATATCC CAGGACTTAG CTCACTCTCT GACACCATGA TCATGGACTC CATTGCTGCC 
TTCCTCGTGT TGCCCAACCG ATTACTGGTG CCCCTTGTGC CTGACCTTCA AGATGTGGCT 
CAGTTGCGTT CCCCTCTGCC CAGGGGCATT ATTCGAATTC ACCTGCTGGC TGCTCGAGGG 
CTGAGTTCCA AGGACAAATA TGTGAAGGGC CTGATTGAGG GCAAGTCAGA CCCATATGCA 
CTTGTGCGTT TGGGTACCCA GACATTCTGC AGTCGTGTCA TTGATGAAGA ACTCAACCCA 
CAGTGGGGAG AGACTTATGA GGTGATGGTA CACGAGGTCC CAGGGCAGGA GATTGAAGTG 
GAGGTGTTCG ACAAGGATCC AGATAAAGAT GACTTTCTGG GCAGAATGAA GCTGGATGTA 
GGGAAGGTGT TACAGGCTAG CGTTCTGGAT GATTGGTTCC CTCTACAAGG TGGGCAAGGC 
CAAGTTCACT TGAGGCTAGA ATGGCTGTCA CTTTTGTCAG ATGCAGAGAA ACTGGAGCAG 
GTTCTACAGT GGAATTGGGG AGTCTCCTCT CGACCAGATC CCCCGTCAGC TGCCATCTTA 
GTTGTCTACC TGGATCGGGC CCAGGATCTT CCTCTGAAGA AGGGGAACAA GGAACCCAAC 
CCTATGGTAC AACTGTCAAT TCAGGATGTG ACTCAGGAGA GCAAGGCTGT CTACAGTACC 
AACTGCCCAG TGTGGGAGGA AGCGTTCCGG TTCTTCCTAC AAGACCCTCA AAGCCAGGAG 
CTCGATGTGC AAGTGAAGGA TGATTCCAGG GCCCTGACTT TAGGAGCACT GACGCTGCCT 
CTGGCCCGCC TGCTGACTGC CCCAGAACTC ATCCTGGACC AGTGGTTCCA GCTCAGCAGC 
TCTGGTCCAA ACTCCAGACT CTATATGAAA CTAGTCATGA GGATCCTGTA CTTGGATTCA 
TCAGAAATAT GCTTCCCCAC GGTGCCTGGT TGTCCTGGTG CTTGGGACGT GGACAGTGAG 
AATCCCCAGA GAGGCAGCAG TGTGGATGCC CCACCTCGAC CCTGTCACAC GACTCCTGAT 
AGCCAGTTTG GGACTGAGCA TGTGCTTCGG ATCCATGTAT TAGAGGCCCA GGACCTGATT 
GCCAAAGACC GTTTCTTGGG GGGACTGGTG AAGGGCAAGT CAGACCCCTA TGTCAAACTA 
AAGTTGGCAG GACGAAGCTT CCGGAGCCAT GTTGTTCGGG AAGATCTCAA TCCCCGCTGG 
AATGAGGTTT TTGAGGTGAT CGTCACATCA GTTCCAGGCC AAGAGCTAGA GGTTGAAGTC 
TTTGACAAGG ACTTGGACAA GGATGATTTT CTGGGCAGGT GTAAAGTGCG TCTCACCACA 
GTCTTAAACA GTGGCTTCCT TGATGAGTGG CTGACCCTGG AGGATGTCCC ATCTGGCCGC 
CTGCACTTGC GCCTGGAGCG TCTCACCCCC CGTCCCACTG CTGCTGAGTT AGAGGAGGTG 
CTGCAGGTGA ATAGTTTGAT CCAGACTCAG AAGAGTGCGG AGCTGGCTGC GGCCCTGCTA 
TCCATCTATA TGGAGCGGGC AGAGGACCTC CCGCTGCGAA AAGGCACCAA GCACCTCAGC 
CCTTATGCTA CTCTCACTGT GGGAGATAGT TCTCATAAAA CCAAGACTAT TTCGCAAACT 
TCAGCCCCTG TCTGGGATGA GAGTGCCTCC TTTCTCATCA GGAAACCACA CACTGAGAGC 
CTAGAGTTGC AGGTTCGGGG TGAGGGCACT GGCGTGCTGG GCTCATTATC CCTGCCCCTC 
TCAGAGCTCC TCGTGGCTGA CCAGCTCTGC TTGGACCGCT GGTTTACACT CAGCAGTGGT 
CAGGGGCAGG TGCTACTGAG AGCACAGCTA GGGATCCTGG TGTCCCAGCA CTCGGGAGTG 
GAAGCTCATA GCCACAGCTA CAGCCACAGC TCCTCATCGC TGAGTGAAGA ACCAGAGCTC 
TCGGGGGGAC CCCCTCACAT CACCTCCTCA GCCCCAGAGC TCCGGCAGCG CCTAACACAT 
GTTGACAGTC CCCTTGAGGC TCCAGCCGGG CCTCTGGGCC AGGTGAAACT GACTCTGTGG 
TACTACAGTG AAGAACGAAA GCTGGTCAGC ATTGTTCATG GTTGCCGGTC CCTTCGACAG 
AATGGACGTG ATCCTCCTGA TCCCTATGTG TCACTGTTGC TACTGCCAGA CAAGAACCGA 
GGCACCAAGA GGAGGACCTC ACAGAAGAAG AGGACCCTGA GTCCTGAATT TAATGAACGG 
TTTGAGTGGG AACTCCCCCT GGATGAGGCC CAGAGACGAA AGCTGGATGT CTCTGTCAAG 
TCTAATTCCT CCTTCATGTC AAGAGAGCGT GAGCTGCTGG GGAAGGTGCA GCTGGACCTA 
GCTGAGACAG ACCTTTCCCA GGGTGTAGCC CGGTGGTATG ACCTGATGGA CAACAAGGAC 
AAGGGCAGCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel microscopy-based assay identifies extended synaptotagmin-1 (ESYT1) as a positive regulator of anoctamin 1 traffic.
Biochimica et biophysica acta1865(2)421-431(2018 Feb)
L?rias JR,Pinto MC,Botelho HM,Awatade NT,Quaresma MC,Silva IAL,Wanitchakool P,Schreiber R,Pepperkok R,Kunzelmann K,Amaral MD

Ca
The EMBO journal37(2)219-234(2018 Jan)
Bian X,Saheki Y,De Camilli P

Control of plasma membrane lipid homeostasis by the extended synaptotagmins.
Nature cell biology18(5)504-15(2016 05)
Saheki Y,Bian X,Schauder CM,Sawaki Y,Surma MA,Klose C,Pincet F,Reinisch KM,De Camilli P

Extended Synaptotagmin Interaction with the Fibroblast Growth Factor Receptor Depends on Receptor Conformation, Not Catalytic Activity.
The Journal of biological chemistry290(26)16142-56(2015 Jun)
Tremblay MG,Herdman C,Guillou F,Mishra PK,Baril J,Bellenfant S,Moss T

Proteomic and genomic evidence implicates the postsynaptic density in schizophrenia.
Molecular psychiatry20(4)424-32(2015 Apr)
F?cking M,Lopez LM,English JA,Dicker P,Wolff A,Brindley E,Wynne K,Cagney G,Cotter DR

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ESYT1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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