ESYT1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ESYT1B
Entrez Gene ID	571083
Full Name	extended synaptotagmin-like protein 1b
Synonyms	fam62a,si:ch211-239j9.4
General protein information	Preferred Names extended synaptotagmin-like protein 1b Names extended synaptotagmin-1 extended synaptotagmin-like protein 1b esyt1 family with sequence similarity 62 (C2 domain containing), member A
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_694639.9	XP_699731.6	extended synaptotagmin-1 isoform X1
XM_017356724.2	XP_017212213.1	extended synaptotagmin-1 isoform X3
XM_017356724.1	XP_017212213.1	extended synaptotagmin-1 isoform X2
XM_021477304.1	XP_021332979.1	extended synaptotagmin-1 isoform X2
XM_694639.8	XP_699731.6	extended synaptotagmin-1 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	esyt1a	XP_695611.3
Arabidopsis thaliana (thale cress)	SYTA	NP_565495.1
Homo sapiens (human)	ESYT1	NP_001171725.1
Pan troglodytes (chimpanzee)	ESYT1	XP_509135.2
	ESYT1	XP_001114269.1
Rattus norvegicus (Norway rat)	Esyt1	NP_058945.2
Danio rerio (zebrafish)	esyt1b	XP_699731.6
Oryza sativa (rice)	Os02g0448400	NP_001046755.1
Oryza sativa (rice)	Os09g0538800	NP_001063800.1
Xenopus tropicalis (tropical clawed frog)	esyt1	XP_002934579.1
Canis lupus familiaris (dog)	ESYT1	XP_005625706.1
Bos taurus (cattle)	ESYT1	NP_001071411.1
Mus musculus (house mouse)	Esyt1	NP_035973.1

Gene Ontology

The following ESYT1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESYT1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa12268	XM_694639.9 Latest version!	Danio rerio extended synaptotagmin-like protein 1b (esyt1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa143141	XM_021477304.1 Latest version!	Danio rerio extended synaptotagmin-like protein 1b (esyt1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa46083	XM_017356724.2 Latest version!	Danio rerio extended synaptotagmin-like protein 1b (esyt1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
ODa46083	XM_017356724.1	Danio rerio extended synaptotagmin-like protein 1b (esyt1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa12268	XM_694639.8	Danio rerio extended synaptotagmin-like protein 1b (esyt1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ESYT1B ( XM_694639.9 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_699731.6 Danio rerio extended synaptotagmin-like protein 1b (esyt1b), transcript variant X1, mRNA.

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CloneID	ODa12268
Clone ID Related Accession (Same CDS sequence)	XM_694639.9 , XM_694639.8
Accession Version	XM_694639.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	extended synaptotagmin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_694639.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCATG TAGATTCAGA GACGACAAAT GACTCGCAGA AGATTACCGA ACCAGCGCAG 
GACAGTTCGT CTGGAGAAAT GGACGTCCCG AAGACCAAAT TGTTCGACGC CAAGGCGATC 
TTGTGGACTT TTGGGAAATG TCTCACCGCC TTGTTGCCCG TGTACCTGGC CGGGTACTAT 
CGGATGAGCA CCAGTTTGGT GGTGTTTGGG ATGATGGTTT ACGCAGGATG GAAACACACG 
CGTGAGGCGA AGGAGGCGAG ACTGAGGTCT GCCATACAGC TAGTAAACGA CGAGCAAGAA 
TATGTGTCCT CCAAATCATT TAGAAGTAAA AGAGACCTGC CTTCTTGGGT CAACTTCCCA 
GATGTGGAGA AGGTTGAATG GCTAAACAAG GTTATCCACC AGGCCTGGCC ATTTATTGGT 
CAGTATTTGG AGAAGCTGCT GACGGAGACC ATTGCTCCGG CCATCCGTGG CTCCAGTGCT 
CATCTGCAGA CCCTCAGCTT CACCAAGATA GACTTCGGTG GTAAGCCAAT GAAAGTGGTC 
GGCGTGAAGG CTCATACAGA AAACGATAAA GGTCAAATTC TGCTTGACGT TTACATCAGT 
TATGTTGGGG ATGTGGAGAT AAACGTGGAG GTGAAGAGAT ACTTCTGCAA AGCTGGTGTG 
AAGGGAATAC AGCTTCATGG AATGATGCGT GTGATTTTGG AGCCGCTGAT CAGCGACGTC 
CCAATAGTAG GAGCTGTGAC CATGTTCTTC ATCCAGAGAC CTAAACTGAC CATTAACTGG 
ACCGGTCTGA CCAACCTGCT GGACATTCCA GGACTCAATG TGATGTCTGA CACCATGATC 
ATGGATGCGA TTGCTTCATT CCTGGTGTTG CCCAATCGCC TGACAGTTCC TCTGGTGGCA 
GACCTGCCTG TGGCACAGCT GCGGTGCCCT TTGCCCAGGG GAGTTGTGCG TATTCACCTG 
CTGGAGGCTG ATAATCTAGC TGCGAAGGAT AATTATGTAA AGGGTGTGAT GGCCGGCATG 
TCTGACCCGT ACGCCATTGT CCGAGTCGGA CCACAAACCT TCAAATCCCA TCACCTGGAC 
AACACGCTCA GTCCCAAATG GGGTGAAGTG TATGAGGTTG TCGTTCATGA GGTGCCTGGT 
CAGGAGCTAG AGGTGGAAGT GTTTGATAAA GATCCCGATC ATGATGATTT CCTGGGCAGG 
ACTAAATTAG ACTTGGGGAT TGTGAAGAAA TCTAAAATAG TTGATGAGTG GTTCAACCTG 
AAGGACACTC AAACAGGACG AGTTCACCTC AAACTGGAGT GGCTGACGCT TGAAACTCAC 
ACAGAAAGAC TGAAAGAGGT TTTGAAGCGA AATGAAAGTG TGGTGAGTAA AGCTGCAGAG 
CCGCCCTCAG CCGCCATCTT GGCAGTGTAT TTGGACAAGG CTGAGGCACT TCCTATGAAG 
AAAGGCAATA AGGATCCCAA TCCGATAGTT CAAATCTCAG TGCAAAATGC TACTCGTGAC 
AGCCGGATTT GCTGGAATAC TGTAAATCCC CAGTGGGAGG ACGCTTTTAC TTTCTTCATC 
AGAGATCCAA ACAATCAGGA CATTAGTGTA CAGGTGAAGG ATAATGACCG TGTTCAGCTT 
TTGGGGAAAA TGTCTATTCC TGCATCCCGT CTCCTCTCTC ATCCCGATCT GTCTATGGAT 
GAATGGTATA ATCTGGAGAA TTCTGGCCCA AAGAGTCGCA TTCACATCAA CACAGTGCTC 
AGGGTTCTCT GGTTGGATGA AGCCGCTGTA ACTGCGTCGC TCCTCTCTAG TGGACCTCTT 
TCAAAGAGCT CACGGCCAGA GAAAACCACT CCCCACTCCA GCTTCGCCAC CGAGGGGCTG 
CTGCGGATCC ACTTGGTCGA GGGTCAGAAT CTGGTGGCTA AAGACAATCT GATGGGTGGG 
ATGGTGAAGG GGAAAAGCGA CCCATATGTC AAGATCCAAA TCGGAGGTGA AACCTTCAAA 
AGTCACGTGA TCAAGGAGAA CCTCAACCCC ACCTGGAATG AGATGTATGA GGTGGTTTTG 
ACGGAGCTTC CGGGTCAAGA GCTCACGTTA GAAGTTTTTG ATAAGGACAT GGATATGAAA 
GATGACTTTA TGGGCAGGCT GAAGATGAGT CTGAGTGACA TCATCAGCTC TCAGTACATT 
AATGAGTGGT TTTCTCTGAG CGATGTAAAG CGTGGTCGTG TTCACCTCGC TCTGGAGTGG 
CTTCCCACTG TAACTAAACC AGAGAAACTA CAGCAGGTGC TGCATTTCCA GTCAAAGAGC 
TCATTTCTGA ATAAGGCAGT GCCGTCTGCA GCTCTCCTGT TTGTGTATGT GGAGCAAGCA 
TATGAGCTTC CTCTCAAGAA GAGCGGGAAG GAGCCTAAAG TTGGAGCTGA GCTGGTCTTA 
GGAGGAACAT CTCGAAAAAC AACGGTGTGT GATCGCACCA GCACTCCTAA ATGGGATGAA 
GCGTTTTACT TTCTGGTTCG AGACCCTTTG AATGAAGACC TTATTGTGAA GTTGTCCCAT 
AACTGGGATT TTTCCGTGGG GTCTGTGGTG ATTCCTATCA AAGAGCTTCT CTCTGAGCCG 
GATCTGCTGC TGGACCAGTG GCTGGATCTG GACGGAGCTT CACCTCAGAG TCAGATTCTG 
CTCAGAGCTC AGCTGAAAAT TCTTTGCCCT AAAAAGATGG AGAGCTCAGA GGAGCAGCAT 
GAGGAGCCCA AACATCATGA AGAGTCCAGC ATCAGAAGAA AACAACAAGA GGAGCTCATG 
CAGAAGTCTA GTATTGAGGA GGTTCCTCCA TCTCCCGTCA GCAGGACGTC CTCAGTTTCT 
GTCCCAGAAG AAGAGGAGGC ACCAGAGGTC ACACAAGTCT CCAGTTCAGA TGACCTTCGA 
CCCCTGCACA CCAGCCCTGA CCCCAGCTTT GGTACAGAGG GTGTTCTGCG GCTCATCCTG 
CTGGAGGCTC AAGATCTGGT GGCGAAGGAT GGTCTGATGG GTGGGATGGT GAAGGGGAAA 
AGTGACCCGT ATGTTAAGAT CCACATCGGT GACACAACAT TTAAGAGTCA CGTAATCAAG 
GAGAACCTCA ACCCCACCTG GAATGAGATG TATGAGCTGG TTCTGACCTC CAGCTCCTCT 
TCTGAAGTGC TCGTAGAGGT TTTCGACAAG GATATGGACA AAGATGACTT TTTAGGGAGG 
ATGAAGATCA GTCTACAGGA GATCATTCAG TCTCAGATCA CTGACAGATG GTTTTCTCTG 
AGTGACGTCA AACATGGTCG TGTTCATTTG ATTCTGGAGT GGCTGAACAC AGTGACAAAA 
CCTGACCCAC TGCAAAAGGC TGTGCAGTTA CAGTCAGATC ATTCATACCT GAACAAAAGT 
GTGCCATCGG CTGCTCTGTT CTTCATCCTC CTGGAAAGAG CTCATAATCT GCCTCTGAAG 
AAGAGTGGAA AAGAGCCTAA AGCTGCTGCA GAGCTGGTGT TAGGAGACAT CACTCACAAA 
ACTAAGGTGT GTGAGAGATC CATGTCTCCT CAGTGGAGCG AAGCTTTTCA CTTCCTGGTT 
CACAAACCTA CTGAGGAGAT TCTTATTATC AAGCTTTCCA GTGCATTCGA GCAGCCGCTG 
GGCTCTCTGG TTCTGCCAAT CAGAGAGCTG CTTTCTAAAA CAGACCTGCT GATGGACCAA 
TGGCTGAGTC TGGATGGAGC AGCTGCTGAC AGTCAGATTC TTCTCAGAGC TCAACTCAAG 
ATTTTGGACT CAAGGCTGGA TTTGAGTGTT TTTCATCGTC AGAAGCCCGT TGTTGAAGAT 
CATTCAACAG GAGAAGAGCA TGAAGAAAAA GGCCCAGCTA CTGCTGAACA TTCACCCGCT 
GCAGTCAGTA AAGAGTCGGA GAAGAAAACA CTGCCAGCAA AGCAAAGCAG GTTGAGTGAA 
CTTGCAGCAT CTACTTTTCC AGTCCCTTTA TCACTGCCTG AAGAACATCA GAAACAAACA 
ACTCACGCCA TTGAGGAGCA ACACACACCT TCGACACCTA AAACACACAT GCCGACTGAG 
GATCTCAGTA AAGCGCCTGA CTCCACTACT AATATTGCTG AACATAAGGA ATCAGCCCAT 
AAAGATCCAC AACACCTGGA TAAAGCAACA GAACCGTCTC ACAAGAGTAG TTCTCCCAGT 
GTTCCTGCTG AGGAGACTAA AGTTACCAGT TCTACAGACA CTCGACCCCA AAAGACGAGC 
CACAACTCCA ACTTTGGCAC CAAGGGGCTG CTGCGGCTCC ACCTGCTGGA GGCTCAGGAT 
CTGGTGGCGA AGGATGGTCT GATGGGTGGG ATGATGAAGG GGAAAAGTGA CCCCTATGTC 
AAGATCCACA TCGGAGACAC AACATTTAAG AGTCACGTGA TCAAGGAAAA CCTCAACCCC 
ACCTGGAATG AGATGTATGA GCTGATTCTG AGTCCTGATC CTAATCTAGA GGTGAAGTTC 
GAGGTCTATG ATAAAGATGT TGATTCTGAT GATTTTCTTG GAAGATTTAA GCTCAGACTT 
GGAGACATCA TCAAATCTCA GTACAATGAT GAGTGGTTTA CATTGAATGA TATCAAACAT 
GGCCGTGTGC ATCTGGTTGT GGAGTGGCTG CCCACCGTCA CCCAGCGGGA CAAACTGGAG 
CAGGTTATGC AGATGCAGAG CTCGCAGTCC TACCAGAACA AGAGTGTGGC TTCAGCAGCG 
CTGCTCTTCA TTCTGTTAGA CAGAGCTCAT CAGCTGCCCC TGAAGAAAAG TGGAAAGGAA 
CCCAAAGCTG CAGCTGAACT TACACTTGGA GGAACGTCTT ATAAATCCAA GGTGTGTGAA 
CGTTCATCTT CTCCTCACTG GAATGAAACT TTTGACTTTC TGGTTCACGA CCCCAAAAAG 
GACGTGCTGG TGATCAAGCT CTCGAGTGCC TGGGATCAAC CAATGGGCTC TTTGGTTCTG 
CCAATCAGAG AGCTGCTTTT AAAACCAGAT CTTCTGCTGG ACCAATGGCT GAGCCTGGAT 
GGAGCTTCAG CACAGAGTCA GATTCTGCTC AGAGCTCAGC TGAAGATCTT GGATTCCAAA 
ATGGCGGCTT TAGTGGCGAT GGGTTCAGGT CCGGTTTTGA GTAACAAGCA AACGGCCACA 
ACAGGACAAA TCCAGCTCTC CATGTCCTTC CAGAAGAAGC TGACGCTGCT CGTCCACAAC 
TGCAGGGGTC TGGTCACATC ATCTAAGGAC AGCTTAGACA CTTACGTCTC CATCATTCTG 
CTGCCAGATA AAAGCAAGGC CACCAAGAGA AAGACCAGTG TGAAGAAGAA AACCCTCAAT 
CCAGAGTTCA ATGAAAAGTT TGAGTTTGAT ATGAGTATGG AAGAGGTTCA GAGACGAGAG 
CTCAGTGTGT GTGTGAAAAA CGCTTCCTCG TCCTTCATGA ACCGAGACAA AGACGTGATC 
GGCCAGGTGC AAATAGACCT GGGACACATT GATTTAATCT CTGGAGTCAC ACAATGGTTT 
GATCTCAAAG AAGAGCAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_699731.6
CDS	282..5765
Translation	MSHVDSETTNDSQKITEPAQDSSSGEMDVPKTKLFDAKAILWTFGKCLTALLPVYLAGYYRMSTSLVVFGMMVYAGWKHTREAKEARLRSAIQLVNDEQEYVSSKSFRSKRDLPSWVNFPDVEKVEWLNKVIHQAWPFIGQYLEKLLTETIAPAIRGSSAHLQTLSFTKIDFGGKPMKVVGVKAHTENDKGQILLDVYISYVGDVEINVEVKRYFCKAGVKGIQLHGMMRVILEPLISDVPIVGAVTMFFIQRPKLTINWTGLTNLLDIPGLNVMSDTMIMDAIASFLVLPNRLTVPLVADLPVAQLRCPLPRGVVRIHLLEADNLAAKDNYVKGVMAGMSDPYAIVRVGPQTFKSHHLDNTLSPKWGEVYEVVVHEVPGQELEVEVFDKDPDHDDFLGRTKLDLGIVKKSKIVDEWFNLKDTQTGRVHLKLEWLTLETHTERLKEVLKRNESVVSKAAEPPSAAILAVYLDKAEALPMKKGNKDPNPIVQISVQNATRDSRICWNTVNPQWEDAFTFFIRDPNNQDISVQVKDNDRVQLLGKMSIPASRLLSHPDLSMDEWYNLENSGPKSRIHINTVLRVLWLDEAAVTASLLSSGPLSKSSRPEKTTPHSSFATEGLLRIHLVEGQNLVAKDNLMGGMVKGKSDPYVKIQIGGETFKSHVIKENLNPTWNEMYEVVLTELPGQELTLEVFDKDMDMKDDFMGRLKMSLSDIISSQYINEWFSLSDVKRGRVHLALEWLPTVTKPEKLQQVLHFQSKSSFLNKAVPSAALLFVYVEQAYELPLKKSGKEPKVGAELVLGGTSRKTTVCDRTSTPKWDEAFYFLVRDPLNEDLIVKLSHNWDFSVGSVVIPIKELLSEPDLLLDQWLDLDGASPQSQILLRAQLKILCPKKMESSEEQHEEPKHHEESSIRRKQQEELMQKSSIEEVPPSPVSRTSSVSVPEEEEAPEVTQVSSSDDLRPLHTSPDPSFGTEGVLRLILLEAQDLVAKDGLMGGMVKGKSDPYVKIHIGDTTFKSHVIKENLNPTWNEMYELVLTSSSSSEVLVEVFDKDMDKDDFLGRMKISLQEIIQSQITDRWFSLSDVKHGRVHLILEWLNTVTKPDPLQKAVQLQSDHSYLNKSVPSAALFFILLERAHNLPLKKSGKEPKAAAELVLGDITHKTKVCERSMSPQWSEAFHFLVHKPTEEILIIKLSSAFEQPLGSLVLPIRELLSKTDLLMDQWLSLDGAAADSQILLRAQLKILDSRLDLSVFHRQKPVVEDHSTGEEHEEKGPATAEHSPAAVSKESEKKTLPAKQSRLSELAASTFPVPLSLPEEHQKQTTHAIEEQHTPSTPKTHMPTEDLSKAPDSTTNIAEHKESAHKDPQHLDKATEPSHKSSSPSVPAEETKVTSSTDTRPQKTSHNSNFGTKGLLRLHLLEAQDLVAKDGLMGGMMKGKSDPYVKIHIGDTTFKSHVIKENLNPTWNEMYELILSPDPNLEVKFEVYDKDVDSDDFLGRFKLRLGDIIKSQYNDEWFTLNDIKHGRVHLVVEWLPTVTQRDKLEQVMQMQSSQSYQNKSVASAALLFILLDRAHQLPLKKSGKEPKAAAELTLGGTSYKSKVCERSSSPHWNETFDFLVHDPKKDVLVIKLSSAWDQPMGSLVLPIRELLLKPDLLLDQWLSLDGASAQSQILLRAQLKILDSKMAALVAMGSGPVLSNKQTATTGQIQLSMSFQKKLTLLVHNCRGLVTSSKDSLDTYVSIILLPDKSKATKRKTSVKKKTLNPEFNEKFEFDMSMEEVQRRELSVCVKNASSSFMNRDKDVIGQVQIDLGHIDLISGVTQWFDLKEEQN

Target ORF information:

RefSeq Version	XM_694639.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio extended synaptotagmin-like protein 1b (esyt1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_694639.9

ORF Insert Sequence:

ATGTCGCATG TAGATTCAGA GACGACAAAT GACTCGCAGA AGATTACCGA ACCAGCGCAG 
GACAGTTCGT CTGGAGAAAT GGACGTCCCG AAGACCAAAT TGTTCGACGC CAAGGCGATC 
TTGTGGACTT TTGGGAAATG TCTCACCGCC TTGTTGCCCG TGTACCTGGC CGGGTACTAT 
CGGATGAGCA CCAGTTTGGT GGTGTTTGGG ATGATGGTTT ACGCAGGATG GAAACACACG 
CGTGAGGCGA AGGAGGCGAG ACTGAGGTCT GCCATACAGC TAGTAAACGA CGAGCAAGAA 
TATGTGTCCT CCAAATCATT TAGAAGTAAA AGAGACCTGC CTTCTTGGGT CAACTTCCCA 
GATGTGGAGA AGGTTGAATG GCTAAACAAG GTTATCCACC AGGCCTGGCC ATTTATTGGT 
CAGTATTTGG AGAAGCTGCT GACGGAGACC ATTGCTCCGG CCATCCGTGG CTCCAGTGCT 
CATCTGCAGA CCCTCAGCTT CACCAAGATA GACTTCGGTG GTAAGCCAAT GAAAGTGGTC 
GGCGTGAAGG CTCATACAGA AAACGATAAA GGTCAAATTC TGCTTGACGT TTACATCAGT 
TATGTTGGGG ATGTGGAGAT AAACGTGGAG GTGAAGAGAT ACTTCTGCAA AGCTGGTGTG 
AAGGGAATAC AGCTTCATGG AATGATGCGT GTGATTTTGG AGCCGCTGAT CAGCGACGTC 
CCAATAGTAG GAGCTGTGAC CATGTTCTTC ATCCAGAGAC CTAAACTGAC CATTAACTGG 
ACCGGTCTGA CCAACCTGCT GGACATTCCA GGACTCAATG TGATGTCTGA CACCATGATC 
ATGGATGCGA TTGCTTCATT CCTGGTGTTG CCCAATCGCC TGACAGTTCC TCTGGTGGCA 
GACCTGCCTG TGGCACAGCT GCGGTGCCCT TTGCCCAGGG GAGTTGTGCG TATTCACCTG 
CTGGAGGCTG ATAATCTAGC TGCGAAGGAT AATTATGTAA AGGGTGTGAT GGCCGGCATG 
TCTGACCCGT ACGCCATTGT CCGAGTCGGA CCACAAACCT TCAAATCCCA TCACCTGGAC 
AACACGCTCA GTCCCAAATG GGGTGAAGTG TATGAGGTTG TCGTTCATGA GGTGCCTGGT 
CAGGAGCTAG AGGTGGAAGT GTTTGATAAA GATCCCGATC ATGATGATTT CCTGGGCAGG 
ACTAAATTAG ACTTGGGGAT TGTGAAGAAA TCTAAAATAG TTGATGAGTG GTTCAACCTG 
AAGGACACTC AAACAGGACG AGTTCACCTC AAACTGGAGT GGCTGACGCT TGAAACTCAC 
ACAGAAAGAC TGAAAGAGGT TTTGAAGCGA AATGAAAGTG TGGTGAGTAA AGCTGCAGAG 
CCGCCCTCAG CCGCCATCTT GGCAGTGTAT TTGGACAAGG CTGAGGCACT TCCTATGAAG 
AAAGGCAATA AGGATCCCAA TCCGATAGTT CAAATCTCAG TGCAAAATGC TACTCGTGAC 
AGCCGGATTT GCTGGAATAC TGTAAATCCC CAGTGGGAGG ACGCTTTTAC TTTCTTCATC 
AGAGATCCAA ACAATCAGGA CATTAGTGTA CAGGTGAAGG ATAATGACCG TGTTCAGCTT 
TTGGGGAAAA TGTCTATTCC TGCATCCCGT CTCCTCTCTC ATCCCGATCT GTCTATGGAT 
GAATGGTATA ATCTGGAGAA TTCTGGCCCA AAGAGTCGCA TTCACATCAA CACAGTGCTC 
AGGGTTCTCT GGTTGGATGA AGCCGCTGTA ACTGCGTCGC TCCTCTCTAG TGGACCTCTT 
TCAAAGAGCT CACGGCCAGA GAAAACCACT CCCCACTCCA GCTTCGCCAC CGAGGGGCTG 
CTGCGGATCC ACTTGGTCGA GGGTCAGAAT CTGGTGGCTA AAGACAATCT GATGGGTGGG 
ATGGTGAAGG GGAAAAGCGA CCCATATGTC AAGATCCAAA TCGGAGGTGA AACCTTCAAA 
AGTCACGTGA TCAAGGAGAA CCTCAACCCC ACCTGGAATG AGATGTATGA GGTGGTTTTG 
ACGGAGCTTC CGGGTCAAGA GCTCACGTTA GAAGTTTTTG ATAAGGACAT GGATATGAAA 
GATGACTTTA TGGGCAGGCT GAAGATGAGT CTGAGTGACA TCATCAGCTC TCAGTACATT 
AATGAGTGGT TTTCTCTGAG CGATGTAAAG CGTGGTCGTG TTCACCTCGC TCTGGAGTGG 
CTTCCCACTG TAACTAAACC AGAGAAACTA CAGCAGGTGC TGCATTTCCA GTCAAAGAGC 
TCATTTCTGA ATAAGGCAGT GCCGTCTGCA GCTCTCCTGT TTGTGTATGT GGAGCAAGCA 
TATGAGCTTC CTCTCAAGAA GAGCGGGAAG GAGCCTAAAG TTGGAGCTGA GCTGGTCTTA 
GGAGGAACAT CTCGAAAAAC AACGGTGTGT GATCGCACCA GCACTCCTAA ATGGGATGAA 
GCGTTTTACT TTCTGGTTCG AGACCCTTTG AATGAAGACC TTATTGTGAA GTTGTCCCAT 
AACTGGGATT TTTCCGTGGG GTCTGTGGTG ATTCCTATCA AAGAGCTTCT CTCTGAGCCG 
GATCTGCTGC TGGACCAGTG GCTGGATCTG GACGGAGCTT CACCTCAGAG TCAGATTCTG 
CTCAGAGCTC AGCTGAAAAT TCTTTGCCCT AAAAAGATGG AGAGCTCAGA GGAGCAGCAT 
GAGGAGCCCA AACATCATGA AGAGTCCAGC ATCAGAAGAA AACAACAAGA GGAGCTCATG 
CAGAAGTCTA GTATTGAGGA GGTTCCTCCA TCTCCCGTCA GCAGGACGTC CTCAGTTTCT 
GTCCCAGAAG AAGAGGAGGC ACCAGAGGTC ACACAAGTCT CCAGTTCAGA TGACCTTCGA 
CCCCTGCACA CCAGCCCTGA CCCCAGCTTT GGTACAGAGG GTGTTCTGCG GCTCATCCTG 
CTGGAGGCTC AAGATCTGGT GGCGAAGGAT GGTCTGATGG GTGGGATGGT GAAGGGGAAA 
AGTGACCCGT ATGTTAAGAT CCACATCGGT GACACAACAT TTAAGAGTCA CGTAATCAAG 
GAGAACCTCA ACCCCACCTG GAATGAGATG TATGAGCTGG TTCTGACCTC CAGCTCCTCT 
TCTGAAGTGC TCGTAGAGGT TTTCGACAAG GATATGGACA AAGATGACTT TTTAGGGAGG 
ATGAAGATCA GTCTACAGGA GATCATTCAG TCTCAGATCA CTGACAGATG GTTTTCTCTG 
AGTGACGTCA AACATGGTCG TGTTCATTTG ATTCTGGAGT GGCTGAACAC AGTGACAAAA 
CCTGACCCAC TGCAAAAGGC TGTGCAGTTA CAGTCAGATC ATTCATACCT GAACAAAAGT 
GTGCCATCGG CTGCTCTGTT CTTCATCCTC CTGGAAAGAG CTCATAATCT GCCTCTGAAG 
AAGAGTGGAA AAGAGCCTAA AGCTGCTGCA GAGCTGGTGT TAGGAGACAT CACTCACAAA 
ACTAAGGTGT GTGAGAGATC CATGTCTCCT CAGTGGAGCG AAGCTTTTCA CTTCCTGGTT 
CACAAACCTA CTGAGGAGAT TCTTATTATC AAGCTTTCCA GTGCATTCGA GCAGCCGCTG 
GGCTCTCTGG TTCTGCCAAT CAGAGAGCTG CTTTCTAAAA CAGACCTGCT GATGGACCAA 
TGGCTGAGTC TGGATGGAGC AGCTGCTGAC AGTCAGATTC TTCTCAGAGC TCAACTCAAG 
ATTTTGGACT CAAGGCTGGA TTTGAGTGTT TTTCATCGTC AGAAGCCCGT TGTTGAAGAT 
CATTCAACAG GAGAAGAGCA TGAAGAAAAA GGCCCAGCTA CTGCTGAACA TTCACCCGCT 
GCAGTCAGTA AAGAGTCGGA GAAGAAAACA CTGCCAGCAA AGCAAAGCAG GTTGAGTGAA 
CTTGCAGCAT CTACTTTTCC AGTCCCTTTA TCACTGCCTG AAGAACATCA GAAACAAACA 
ACTCACGCCA TTGAGGAGCA ACACACACCT TCGACACCTA AAACACACAT GCCGACTGAG 
GATCTCAGTA AAGCGCCTGA CTCCACTACT AATATTGCTG AACATAAGGA ATCAGCCCAT 
AAAGATCCAC AACACCTGGA TAAAGCAACA GAACCGTCTC ACAAGAGTAG TTCTCCCAGT 
GTTCCTGCTG AGGAGACTAA AGTTACCAGT TCTACAGACA CTCGACCCCA AAAGACGAGC 
CACAACTCCA ACTTTGGCAC CAAGGGGCTG CTGCGGCTCC ACCTGCTGGA GGCTCAGGAT 
CTGGTGGCGA AGGATGGTCT GATGGGTGGG ATGATGAAGG GGAAAAGTGA CCCCTATGTC 
AAGATCCACA TCGGAGACAC AACATTTAAG AGTCACGTGA TCAAGGAAAA CCTCAACCCC 
ACCTGGAATG AGATGTATGA GCTGATTCTG AGTCCTGATC CTAATCTAGA GGTGAAGTTC 
GAGGTCTATG ATAAAGATGT TGATTCTGAT GATTTTCTTG GAAGATTTAA GCTCAGACTT 
GGAGACATCA TCAAATCTCA GTACAATGAT GAGTGGTTTA CATTGAATGA TATCAAACAT 
GGCCGTGTGC ATCTGGTTGT GGAGTGGCTG CCCACCGTCA CCCAGCGGGA CAAACTGGAG 
CAGGTTATGC AGATGCAGAG CTCGCAGTCC TACCAGAACA AGAGTGTGGC TTCAGCAGCG 
CTGCTCTTCA TTCTGTTAGA CAGAGCTCAT CAGCTGCCCC TGAAGAAAAG TGGAAAGGAA 
CCCAAAGCTG CAGCTGAACT TACACTTGGA GGAACGTCTT ATAAATCCAA GGTGTGTGAA 
CGTTCATCTT CTCCTCACTG GAATGAAACT TTTGACTTTC TGGTTCACGA CCCCAAAAAG 
GACGTGCTGG TGATCAAGCT CTCGAGTGCC TGGGATCAAC CAATGGGCTC TTTGGTTCTG 
CCAATCAGAG AGCTGCTTTT AAAACCAGAT CTTCTGCTGG ACCAATGGCT GAGCCTGGAT 
GGAGCTTCAG CACAGAGTCA GATTCTGCTC AGAGCTCAGC TGAAGATCTT GGATTCCAAA 
ATGGCGGCTT TAGTGGCGAT GGGTTCAGGT CCGGTTTTGA GTAACAAGCA AACGGCCACA 
ACAGGACAAA TCCAGCTCTC CATGTCCTTC CAGAAGAAGC TGACGCTGCT CGTCCACAAC 
TGCAGGGGTC TGGTCACATC ATCTAAGGAC AGCTTAGACA CTTACGTCTC CATCATTCTG 
CTGCCAGATA AAAGCAAGGC CACCAAGAGA AAGACCAGTG TGAAGAAGAA AACCCTCAAT 
CCAGAGTTCA ATGAAAAGTT TGAGTTTGAT ATGAGTATGG AAGAGGTTCA GAGACGAGAG 
CTCAGTGTGT GTGTGAAAAA CGCTTCCTCG TCCTTCATGA ACCGAGACAA AGACGTGATC 
GGCCAGGTGC AAATAGACCT GGGACACATT GATTTAATCT CTGGAGTCAC ACAATGGTTT 
GATCTCAAAG AAGAGCAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESYT1B ( XM_694639.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_699731.6 Danio rerio extended synaptotagmin-like protein 1b (esyt1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa12268
Clone ID Related Accession (Same CDS sequence)	XM_694639.9 , XM_694639.8
Accession Version	XM_694639.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	extended synaptotagmin-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_694639.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCATG TAGATTCAGA GACGACAAAT GACTCGCAGA AGATTACCGA ACCAGCGCAG 
GACAGTTCGT CTGGAGAAAT GGACGTCCCG AAGACCAAAT TGTTCGACGC CAAGGCGATC 
TTGTGGACTT TTGGGAAATG TCTCACCGCC TTGTTGCCCG TGTACCTGGC CGGGTACTAT 
CGGATGAGCA CCAGTTTGGT GGTGTTTGGG ATGATGGTTT ACGCAGGATG GAAACACACG 
CGTGAGGCGA AGGAGGCGAG ACTGAGGTCT GCCATACAGC TAGTAAACGA CGAGCAAGAA 
TATGTGTCCT CCAAATCATT TAGAAGTAAA AGAGACCTGC CTTCTTGGGT CAACTTCCCA 
GATGTGGAGA AGGTTGAATG GCTAAACAAG GTTATCCACC AGGCCTGGCC ATTTATTGGT 
CAGTATTTGG AGAAGCTGCT GACGGAGACC ATTGCTCCGG CCATCCGTGG CTCCAGTGCT 
CATCTGCAGA CCCTCAGCTT CACCAAGATA GACTTCGGTG GTAAGCCAAT GAAAGTGGTC 
GGCGTGAAGG CTCATACAGA AAACGATAAA GGTCAAATTC TGCTTGACGT TTACATCAGT 
TATGTTGGGG ATGTGGAGAT AAACGTGGAG GTGAAGAGAT ACTTCTGCAA AGCTGGTGTG 
AAGGGAATAC AGCTTCATGG AATGATGCGT GTGATTTTGG AGCCGCTGAT CAGCGACGTC 
CCAATAGTAG GAGCTGTGAC CATGTTCTTC ATCCAGAGAC CTAAACTGAC CATTAACTGG 
ACCGGTCTGA CCAACCTGCT GGACATTCCA GGACTCAATG TGATGTCTGA CACCATGATC 
ATGGATGCGA TTGCTTCATT CCTGGTGTTG CCCAATCGCC TGACAGTTCC TCTGGTGGCA 
GACCTGCCTG TGGCACAGCT GCGGTGCCCT TTGCCCAGGG GAGTTGTGCG TATTCACCTG 
CTGGAGGCTG ATAATCTAGC TGCGAAGGAT AATTATGTAA AGGGTGTGAT GGCCGGCATG 
TCTGACCCGT ACGCCATTGT CCGAGTCGGA CCACAAACCT TCAAATCCCA TCACCTGGAC 
AACACGCTCA GTCCCAAATG GGGTGAAGTG TATGAGGTTG TCGTTCATGA GGTGCCTGGT 
CAGGAGCTAG AGGTGGAAGT GTTTGATAAA GATCCCGATC ATGATGATTT CCTGGGCAGG 
ACTAAATTAG ACTTGGGGAT TGTGAAGAAA TCTAAAATAG TTGATGAGTG GTTCAACCTG 
AAGGACACTC AAACAGGACG AGTTCACCTC AAACTGGAGT GGCTGACGCT TGAAACTCAC 
ACAGAAAGAC TGAAAGAGGT TTTGAAGCGA AATGAAAGTG TGGTGAGTAA AGCTGCAGAG 
CCGCCCTCAG CCGCCATCTT GGCAGTGTAT TTGGACAAGG CTGAGGCACT TCCTATGAAG 
AAAGGCAATA AGGATCCCAA TCCGATAGTT CAAATCTCAG TGCAAAATGC TACTCGTGAC 
AGCCGGATTT GCTGGAATAC TGTAAATCCC CAGTGGGAGG ACGCTTTTAC TTTCTTCATC 
AGAGATCCAA ACAATCAGGA CATTAGTGTA CAGGTGAAGG ATAATGACCG TGTTCAGCTT 
TTGGGGAAAA TGTCTATTCC TGCATCCCGT CTCCTCTCTC ATCCCGATCT GTCTATGGAT 
GAATGGTATA ATCTGGAGAA TTCTGGCCCA AAGAGTCGCA TTCACATCAA CACAGTGCTC 
AGGGTTCTCT GGTTGGATGA AGCCGCTGTA ACTGCGTCGC TCCTCTCTAG TGGACCTCTT 
TCAAAGAGCT CACGGCCAGA GAAAACCACT CCCCACTCCA GCTTCGCCAC CGAGGGGCTG 
CTGCGGATCC ACTTGGTCGA GGGTCAGAAT CTGGTGGCTA AAGACAATCT GATGGGTGGG 
ATGGTGAAGG GGAAAAGCGA CCCATATGTC AAGATCCAAA TCGGAGGTGA AACCTTCAAA 
AGTCACGTGA TCAAGGAGAA CCTCAACCCC ACCTGGAATG AGATGTATGA GGTGGTTTTG 
ACGGAGCTTC CGGGTCAAGA GCTCACGTTA GAAGTTTTTG ATAAGGACAT GGATATGAAA 
GATGACTTTA TGGGCAGGCT GAAGATGAGT CTGAGTGACA TCATCAGCTC TCAGTACATT 
AATGAGTGGT TTTCTCTGAG CGATGTAAAG CGTGGTCGTG TTCACCTCGC TCTGGAGTGG 
CTTCCCACTG TAACTAAACC AGAGAAACTA CAGCAGGTGC TGCATTTCCA GTCAAAGAGC 
TCATTTCTGA ATAAGGCAGT GCCGTCTGCA GCTCTCCTGT TTGTGTATGT GGAGCAAGCA 
TATGAGCTTC CTCTCAAGAA GAGCGGGAAG GAGCCTAAAG TTGGAGCTGA GCTGGTCTTA 
GGAGGAACAT CTCGAAAAAC AACGGTGTGT GATCGCACCA GCACTCCTAA ATGGGATGAA 
GCGTTTTACT TTCTGGTTCG AGACCCTTTG AATGAAGACC TTATTGTGAA GTTGTCCCAT 
AACTGGGATT TTTCCGTGGG GTCTGTGGTG ATTCCTATCA AAGAGCTTCT CTCTGAGCCG 
GATCTGCTGC TGGACCAGTG GCTGGATCTG GACGGAGCTT CACCTCAGAG TCAGATTCTG 
CTCAGAGCTC AGCTGAAAAT TCTTTGCCCT AAAAAGATGG AGAGCTCAGA GGAGCAGCAT 
GAGGAGCCCA AACATCATGA AGAGTCCAGC ATCAGAAGAA AACAACAAGA GGAGCTCATG 
CAGAAGTCTA GTATTGAGGA GGTTCCTCCA TCTCCCGTCA GCAGGACGTC CTCAGTTTCT 
GTCCCAGAAG AAGAGGAGGC ACCAGAGGTC ACACAAGTCT CCAGTTCAGA TGACCTTCGA 
CCCCTGCACA CCAGCCCTGA CCCCAGCTTT GGTACAGAGG GTGTTCTGCG GCTCATCCTG 
CTGGAGGCTC AAGATCTGGT GGCGAAGGAT GGTCTGATGG GTGGGATGGT GAAGGGGAAA 
AGTGACCCGT ATGTTAAGAT CCACATCGGT GACACAACAT TTAAGAGTCA CGTAATCAAG 
GAGAACCTCA ACCCCACCTG GAATGAGATG TATGAGCTGG TTCTGACCTC CAGCTCCTCT 
TCTGAAGTGC TCGTAGAGGT TTTCGACAAG GATATGGACA AAGATGACTT TTTAGGGAGG 
ATGAAGATCA GTCTACAGGA GATCATTCAG TCTCAGATCA CTGACAGATG GTTTTCTCTG 
AGTGACGTCA AACATGGTCG TGTTCATTTG ATTCTGGAGT GGCTGAACAC AGTGACAAAA 
CCTGACCCAC TGCAAAAGGC TGTGCAGTTA CAGTCAGATC ATTCATACCT GAACAAAAGT 
GTGCCATCGG CTGCTCTGTT CTTCATCCTC CTGGAAAGAG CTCATAATCT GCCTCTGAAG 
AAGAGTGGAA AAGAGCCTAA AGCTGCTGCA GAGCTGGTGT TAGGAGACAT CACTCACAAA 
ACTAAGGTGT GTGAGAGATC CATGTCTCCT CAGTGGAGCG AAGCTTTTCA CTTCCTGGTT 
CACAAACCTA CTGAGGAGAT TCTTATTATC AAGCTTTCCA GTGCATTCGA GCAGCCGCTG 
GGCTCTCTGG TTCTGCCAAT CAGAGAGCTG CTTTCTAAAA CAGACCTGCT GATGGACCAA 
TGGCTGAGTC TGGATGGAGC AGCTGCTGAC AGTCAGATTC TTCTCAGAGC TCAACTCAAG 
ATTTTGGACT CAAGGCTGGA TTTGAGTGTT TTTCATCGTC AGAAGCCCGT TGTTGAAGAT 
CATTCAACAG GAGAAGAGCA TGAAGAAAAA GGCCCAGCTA CTGCTGAACA TTCACCCGCT 
GCAGTCAGTA AAGAGTCGGA GAAGAAAACA CTGCCAGCAA AGCAAAGCAG GTTGAGTGAA 
CTTGCAGCAT CTACTTTTCC AGTCCCTTTA TCACTGCCTG AAGAACATCA GAAACAAACA 
ACTCACGCCA TTGAGGAGCA ACACACACCT TCGACACCTA AAACACACAT GCCGACTGAG 
GATCTCAGTA AAGCGCCTGA CTCCACTACT AATATTGCTG AACATAAGGA ATCAGCCCAT 
AAAGATCCAC AACACCTGGA TAAAGCAACA GAACCGTCTC ACAAGAGTAG TTCTCCCAGT 
GTTCCTGCTG AGGAGACTAA AGTTACCAGT TCTACAGACA CTCGACCCCA AAAGACGAGC 
CACAACTCCA ACTTTGGCAC CAAGGGGCTG CTGCGGCTCC ACCTGCTGGA GGCTCAGGAT 
CTGGTGGCGA AGGATGGTCT GATGGGTGGG ATGATGAAGG GGAAAAGTGA CCCCTATGTC 
AAGATCCACA TCGGAGACAC AACATTTAAG AGTCACGTGA TCAAGGAAAA CCTCAACCCC 
ACCTGGAATG AGATGTATGA GCTGATTCTG AGTCCTGATC CTAATCTAGA GGTGAAGTTC 
GAGGTCTATG ATAAAGATGT TGATTCTGAT GATTTTCTTG GAAGATTTAA GCTCAGACTT 
GGAGACATCA TCAAATCTCA GTACAATGAT GAGTGGTTTA CATTGAATGA TATCAAACAT 
GGCCGTGTGC ATCTGGTTGT GGAGTGGCTG CCCACCGTCA CCCAGCGGGA CAAACTGGAG 
CAGGTTATGC AGATGCAGAG CTCGCAGTCC TACCAGAACA AGAGTGTGGC TTCAGCAGCG 
CTGCTCTTCA TTCTGTTAGA CAGAGCTCAT CAGCTGCCCC TGAAGAAAAG TGGAAAGGAA 
CCCAAAGCTG CAGCTGAACT TACACTTGGA GGAACGTCTT ATAAATCCAA GGTGTGTGAA 
CGTTCATCTT CTCCTCACTG GAATGAAACT TTTGACTTTC TGGTTCACGA CCCCAAAAAG 
GACGTGCTGG TGATCAAGCT CTCGAGTGCC TGGGATCAAC CAATGGGCTC TTTGGTTCTG 
CCAATCAGAG AGCTGCTTTT AAAACCAGAT CTTCTGCTGG ACCAATGGCT GAGCCTGGAT 
GGAGCTTCAG CACAGAGTCA GATTCTGCTC AGAGCTCAGC TGAAGATCTT GGATTCCAAA 
ATGGCGGCTT TAGTGGCGAT GGGTTCAGGT CCGGTTTTGA GTAACAAGCA AACGGCCACA 
ACAGGACAAA TCCAGCTCTC CATGTCCTTC CAGAAGAAGC TGACGCTGCT CGTCCACAAC 
TGCAGGGGTC TGGTCACATC ATCTAAGGAC AGCTTAGACA CTTACGTCTC CATCATTCTG 
CTGCCAGATA AAAGCAAGGC CACCAAGAGA AAGACCAGTG TGAAGAAGAA AACCCTCAAT 
CCAGAGTTCA ATGAAAAGTT TGAGTTTGAT ATGAGTATGG AAGAGGTTCA GAGACGAGAG 
CTCAGTGTGT GTGTGAAAAA CGCTTCCTCG TCCTTCATGA ACCGAGACAA AGACGTGATC 
GGCCAGGTGC AAATAGACCT GGGACACATT GATTTAATCT CTGGAGTCAC ACAATGGTTT 
GATCTCAAAG AAGAGCAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_699731.6
CDS	371..5854
Translation	MSHVDSETTNDSQKITEPAQDSSSGEMDVPKTKLFDAKAILWTFGKCLTALLPVYLAGYYRMSTSLVVFGMMVYAGWKHTREAKEARLRSAIQLVNDEQEYVSSKSFRSKRDLPSWVNFPDVEKVEWLNKVIHQAWPFIGQYLEKLLTETIAPAIRGSSAHLQTLSFTKIDFGGKPMKVVGVKAHTENDKGQILLDVYISYVGDVEINVEVKRYFCKAGVKGIQLHGMMRVILEPLISDVPIVGAVTMFFIQRPKLTINWTGLTNLLDIPGLNVMSDTMIMDAIASFLVLPNRLTVPLVADLPVAQLRCPLPRGVVRIHLLEADNLAAKDNYVKGVMAGMSDPYAIVRVGPQTFKSHHLDNTLSPKWGEVYEVVVHEVPGQELEVEVFDKDPDHDDFLGRTKLDLGIVKKSKIVDEWFNLKDTQTGRVHLKLEWLTLETHTERLKEVLKRNESVVSKAAEPPSAAILAVYLDKAEALPMKKGNKDPNPIVQISVQNATRDSRICWNTVNPQWEDAFTFFIRDPNNQDISVQVKDNDRVQLLGKMSIPASRLLSHPDLSMDEWYNLENSGPKSRIHINTVLRVLWLDEAAVTASLLSSGPLSKSSRPEKTTPHSSFATEGLLRIHLVEGQNLVAKDNLMGGMVKGKSDPYVKIQIGGETFKSHVIKENLNPTWNEMYEVVLTELPGQELTLEVFDKDMDMKDDFMGRLKMSLSDIISSQYINEWFSLSDVKRGRVHLALEWLPTVTKPEKLQQVLHFQSKSSFLNKAVPSAALLFVYVEQAYELPLKKSGKEPKVGAELVLGGTSRKTTVCDRTSTPKWDEAFYFLVRDPLNEDLIVKLSHNWDFSVGSVVIPIKELLSEPDLLLDQWLDLDGASPQSQILLRAQLKILCPKKMESSEEQHEEPKHHEESSIRRKQQEELMQKSSIEEVPPSPVSRTSSVSVPEEEEAPEVTQVSSSDDLRPLHTSPDPSFGTEGVLRLILLEAQDLVAKDGLMGGMVKGKSDPYVKIHIGDTTFKSHVIKENLNPTWNEMYELVLTSSSSSEVLVEVFDKDMDKDDFLGRMKISLQEIIQSQITDRWFSLSDVKHGRVHLILEWLNTVTKPDPLQKAVQLQSDHSYLNKSVPSAALFFILLERAHNLPLKKSGKEPKAAAELVLGDITHKTKVCERSMSPQWSEAFHFLVHKPTEEILIIKLSSAFEQPLGSLVLPIRELLSKTDLLMDQWLSLDGAAADSQILLRAQLKILDSRLDLSVFHRQKPVVEDHSTGEEHEEKGPATAEHSPAAVSKESEKKTLPAKQSRLSELAASTFPVPLSLPEEHQKQTTHAIEEQHTPSTPKTHMPTEDLSKAPDSTTNIAEHKESAHKDPQHLDKATEPSHKSSSPSVPAEETKVTSSTDTRPQKTSHNSNFGTKGLLRLHLLEAQDLVAKDGLMGGMMKGKSDPYVKIHIGDTTFKSHVIKENLNPTWNEMYELILSPDPNLEVKFEVYDKDVDSDDFLGRFKLRLGDIIKSQYNDEWFTLNDIKHGRVHLVVEWLPTVTQRDKLEQVMQMQSSQSYQNKSVASAALLFILLDRAHQLPLKKSGKEPKAAAELTLGGTSYKSKVCERSSSPHWNETFDFLVHDPKKDVLVIKLSSAWDQPMGSLVLPIRELLLKPDLLLDQWLSLDGASAQSQILLRAQLKILDSKMAALVAMGSGPVLSNKQTATTGQIQLSMSFQKKLTLLVHNCRGLVTSSKDSLDTYVSIILLPDKSKATKRKTSVKKKTLNPEFNEKFEFDMSMEEVQRRELSVCVKNASSSFMNRDKDVIGQVQIDLGHIDLISGVTQWFDLKEEQN

Target ORF information:

RefSeq Version	XM_694639.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio extended synaptotagmin-like protein 1b (esyt1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_694639.8

ORF Insert Sequence:

ATGTCGCATG TAGATTCAGA GACGACAAAT GACTCGCAGA AGATTACCGA ACCAGCGCAG 
GACAGTTCGT CTGGAGAAAT GGACGTCCCG AAGACCAAAT TGTTCGACGC CAAGGCGATC 
TTGTGGACTT TTGGGAAATG TCTCACCGCC TTGTTGCCCG TGTACCTGGC CGGGTACTAT 
CGGATGAGCA CCAGTTTGGT GGTGTTTGGG ATGATGGTTT ACGCAGGATG GAAACACACG 
CGTGAGGCGA AGGAGGCGAG ACTGAGGTCT GCCATACAGC TAGTAAACGA CGAGCAAGAA 
TATGTGTCCT CCAAATCATT TAGAAGTAAA AGAGACCTGC CTTCTTGGGT CAACTTCCCA 
GATGTGGAGA AGGTTGAATG GCTAAACAAG GTTATCCACC AGGCCTGGCC ATTTATTGGT 
CAGTATTTGG AGAAGCTGCT GACGGAGACC ATTGCTCCGG CCATCCGTGG CTCCAGTGCT 
CATCTGCAGA CCCTCAGCTT CACCAAGATA GACTTCGGTG GTAAGCCAAT GAAAGTGGTC 
GGCGTGAAGG CTCATACAGA AAACGATAAA GGTCAAATTC TGCTTGACGT TTACATCAGT 
TATGTTGGGG ATGTGGAGAT AAACGTGGAG GTGAAGAGAT ACTTCTGCAA AGCTGGTGTG 
AAGGGAATAC AGCTTCATGG AATGATGCGT GTGATTTTGG AGCCGCTGAT CAGCGACGTC 
CCAATAGTAG GAGCTGTGAC CATGTTCTTC ATCCAGAGAC CTAAACTGAC CATTAACTGG 
ACCGGTCTGA CCAACCTGCT GGACATTCCA GGACTCAATG TGATGTCTGA CACCATGATC 
ATGGATGCGA TTGCTTCATT CCTGGTGTTG CCCAATCGCC TGACAGTTCC TCTGGTGGCA 
GACCTGCCTG TGGCACAGCT GCGGTGCCCT TTGCCCAGGG GAGTTGTGCG TATTCACCTG 
CTGGAGGCTG ATAATCTAGC TGCGAAGGAT AATTATGTAA AGGGTGTGAT GGCCGGCATG 
TCTGACCCGT ACGCCATTGT CCGAGTCGGA CCACAAACCT TCAAATCCCA TCACCTGGAC 
AACACGCTCA GTCCCAAATG GGGTGAAGTG TATGAGGTTG TCGTTCATGA GGTGCCTGGT 
CAGGAGCTAG AGGTGGAAGT GTTTGATAAA GATCCCGATC ATGATGATTT CCTGGGCAGG 
ACTAAATTAG ACTTGGGGAT TGTGAAGAAA TCTAAAATAG TTGATGAGTG GTTCAACCTG 
AAGGACACTC AAACAGGACG AGTTCACCTC AAACTGGAGT GGCTGACGCT TGAAACTCAC 
ACAGAAAGAC TGAAAGAGGT TTTGAAGCGA AATGAAAGTG TGGTGAGTAA AGCTGCAGAG 
CCGCCCTCAG CCGCCATCTT GGCAGTGTAT TTGGACAAGG CTGAGGCACT TCCTATGAAG 
AAAGGCAATA AGGATCCCAA TCCGATAGTT CAAATCTCAG TGCAAAATGC TACTCGTGAC 
AGCCGGATTT GCTGGAATAC TGTAAATCCC CAGTGGGAGG ACGCTTTTAC TTTCTTCATC 
AGAGATCCAA ACAATCAGGA CATTAGTGTA CAGGTGAAGG ATAATGACCG TGTTCAGCTT 
TTGGGGAAAA TGTCTATTCC TGCATCCCGT CTCCTCTCTC ATCCCGATCT GTCTATGGAT 
GAATGGTATA ATCTGGAGAA TTCTGGCCCA AAGAGTCGCA TTCACATCAA CACAGTGCTC 
AGGGTTCTCT GGTTGGATGA AGCCGCTGTA ACTGCGTCGC TCCTCTCTAG TGGACCTCTT 
TCAAAGAGCT CACGGCCAGA GAAAACCACT CCCCACTCCA GCTTCGCCAC CGAGGGGCTG 
CTGCGGATCC ACTTGGTCGA GGGTCAGAAT CTGGTGGCTA AAGACAATCT GATGGGTGGG 
ATGGTGAAGG GGAAAAGCGA CCCATATGTC AAGATCCAAA TCGGAGGTGA AACCTTCAAA 
AGTCACGTGA TCAAGGAGAA CCTCAACCCC ACCTGGAATG AGATGTATGA GGTGGTTTTG 
ACGGAGCTTC CGGGTCAAGA GCTCACGTTA GAAGTTTTTG ATAAGGACAT GGATATGAAA 
GATGACTTTA TGGGCAGGCT GAAGATGAGT CTGAGTGACA TCATCAGCTC TCAGTACATT 
AATGAGTGGT TTTCTCTGAG CGATGTAAAG CGTGGTCGTG TTCACCTCGC TCTGGAGTGG 
CTTCCCACTG TAACTAAACC AGAGAAACTA CAGCAGGTGC TGCATTTCCA GTCAAAGAGC 
TCATTTCTGA ATAAGGCAGT GCCGTCTGCA GCTCTCCTGT TTGTGTATGT GGAGCAAGCA 
TATGAGCTTC CTCTCAAGAA GAGCGGGAAG GAGCCTAAAG TTGGAGCTGA GCTGGTCTTA 
GGAGGAACAT CTCGAAAAAC AACGGTGTGT GATCGCACCA GCACTCCTAA ATGGGATGAA 
GCGTTTTACT TTCTGGTTCG AGACCCTTTG AATGAAGACC TTATTGTGAA GTTGTCCCAT 
AACTGGGATT TTTCCGTGGG GTCTGTGGTG ATTCCTATCA AAGAGCTTCT CTCTGAGCCG 
GATCTGCTGC TGGACCAGTG GCTGGATCTG GACGGAGCTT CACCTCAGAG TCAGATTCTG 
CTCAGAGCTC AGCTGAAAAT TCTTTGCCCT AAAAAGATGG AGAGCTCAGA GGAGCAGCAT 
GAGGAGCCCA AACATCATGA AGAGTCCAGC ATCAGAAGAA AACAACAAGA GGAGCTCATG 
CAGAAGTCTA GTATTGAGGA GGTTCCTCCA TCTCCCGTCA GCAGGACGTC CTCAGTTTCT 
GTCCCAGAAG AAGAGGAGGC ACCAGAGGTC ACACAAGTCT CCAGTTCAGA TGACCTTCGA 
CCCCTGCACA CCAGCCCTGA CCCCAGCTTT GGTACAGAGG GTGTTCTGCG GCTCATCCTG 
CTGGAGGCTC AAGATCTGGT GGCGAAGGAT GGTCTGATGG GTGGGATGGT GAAGGGGAAA 
AGTGACCCGT ATGTTAAGAT CCACATCGGT GACACAACAT TTAAGAGTCA CGTAATCAAG 
GAGAACCTCA ACCCCACCTG GAATGAGATG TATGAGCTGG TTCTGACCTC CAGCTCCTCT 
TCTGAAGTGC TCGTAGAGGT TTTCGACAAG GATATGGACA AAGATGACTT TTTAGGGAGG 
ATGAAGATCA GTCTACAGGA GATCATTCAG TCTCAGATCA CTGACAGATG GTTTTCTCTG 
AGTGACGTCA AACATGGTCG TGTTCATTTG ATTCTGGAGT GGCTGAACAC AGTGACAAAA 
CCTGACCCAC TGCAAAAGGC TGTGCAGTTA CAGTCAGATC ATTCATACCT GAACAAAAGT 
GTGCCATCGG CTGCTCTGTT CTTCATCCTC CTGGAAAGAG CTCATAATCT GCCTCTGAAG 
AAGAGTGGAA AAGAGCCTAA AGCTGCTGCA GAGCTGGTGT TAGGAGACAT CACTCACAAA 
ACTAAGGTGT GTGAGAGATC CATGTCTCCT CAGTGGAGCG AAGCTTTTCA CTTCCTGGTT 
CACAAACCTA CTGAGGAGAT TCTTATTATC AAGCTTTCCA GTGCATTCGA GCAGCCGCTG 
GGCTCTCTGG TTCTGCCAAT CAGAGAGCTG CTTTCTAAAA CAGACCTGCT GATGGACCAA 
TGGCTGAGTC TGGATGGAGC AGCTGCTGAC AGTCAGATTC TTCTCAGAGC TCAACTCAAG 
ATTTTGGACT CAAGGCTGGA TTTGAGTGTT TTTCATCGTC AGAAGCCCGT TGTTGAAGAT 
CATTCAACAG GAGAAGAGCA TGAAGAAAAA GGCCCAGCTA CTGCTGAACA TTCACCCGCT 
GCAGTCAGTA AAGAGTCGGA GAAGAAAACA CTGCCAGCAA AGCAAAGCAG GTTGAGTGAA 
CTTGCAGCAT CTACTTTTCC AGTCCCTTTA TCACTGCCTG AAGAACATCA GAAACAAACA 
ACTCACGCCA TTGAGGAGCA ACACACACCT TCGACACCTA AAACACACAT GCCGACTGAG 
GATCTCAGTA AAGCGCCTGA CTCCACTACT AATATTGCTG AACATAAGGA ATCAGCCCAT 
AAAGATCCAC AACACCTGGA TAAAGCAACA GAACCGTCTC ACAAGAGTAG TTCTCCCAGT 
GTTCCTGCTG AGGAGACTAA AGTTACCAGT TCTACAGACA CTCGACCCCA AAAGACGAGC 
CACAACTCCA ACTTTGGCAC CAAGGGGCTG CTGCGGCTCC ACCTGCTGGA GGCTCAGGAT 
CTGGTGGCGA AGGATGGTCT GATGGGTGGG ATGATGAAGG GGAAAAGTGA CCCCTATGTC 
AAGATCCACA TCGGAGACAC AACATTTAAG AGTCACGTGA TCAAGGAAAA CCTCAACCCC 
ACCTGGAATG AGATGTATGA GCTGATTCTG AGTCCTGATC CTAATCTAGA GGTGAAGTTC 
GAGGTCTATG ATAAAGATGT TGATTCTGAT GATTTTCTTG GAAGATTTAA GCTCAGACTT 
GGAGACATCA TCAAATCTCA GTACAATGAT GAGTGGTTTA CATTGAATGA TATCAAACAT 
GGCCGTGTGC ATCTGGTTGT GGAGTGGCTG CCCACCGTCA CCCAGCGGGA CAAACTGGAG 
CAGGTTATGC AGATGCAGAG CTCGCAGTCC TACCAGAACA AGAGTGTGGC TTCAGCAGCG 
CTGCTCTTCA TTCTGTTAGA CAGAGCTCAT CAGCTGCCCC TGAAGAAAAG TGGAAAGGAA 
CCCAAAGCTG CAGCTGAACT TACACTTGGA GGAACGTCTT ATAAATCCAA GGTGTGTGAA 
CGTTCATCTT CTCCTCACTG GAATGAAACT TTTGACTTTC TGGTTCACGA CCCCAAAAAG 
GACGTGCTGG TGATCAAGCT CTCGAGTGCC TGGGATCAAC CAATGGGCTC TTTGGTTCTG 
CCAATCAGAG AGCTGCTTTT AAAACCAGAT CTTCTGCTGG ACCAATGGCT GAGCCTGGAT 
GGAGCTTCAG CACAGAGTCA GATTCTGCTC AGAGCTCAGC TGAAGATCTT GGATTCCAAA 
ATGGCGGCTT TAGTGGCGAT GGGTTCAGGT CCGGTTTTGA GTAACAAGCA AACGGCCACA 
ACAGGACAAA TCCAGCTCTC CATGTCCTTC CAGAAGAAGC TGACGCTGCT CGTCCACAAC 
TGCAGGGGTC TGGTCACATC ATCTAAGGAC AGCTTAGACA CTTACGTCTC CATCATTCTG 
CTGCCAGATA AAAGCAAGGC CACCAAGAGA AAGACCAGTG TGAAGAAGAA AACCCTCAAT 
CCAGAGTTCA ATGAAAAGTT TGAGTTTGAT ATGAGTATGG AAGAGGTTCA GAGACGAGAG 
CTCAGTGTGT GTGTGAAAAA CGCTTCCTCG TCCTTCATGA ACCGAGACAA AGACGTGATC 
GGCCAGGTGC AAATAGACCT GGGACACATT GATTTAATCT CTGGAGTCAC ACAATGGTTT 
GATCTCAAAG AAGAGCAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESYT1B ( XM_021477304.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021332979.1 Danio rerio extended synaptotagmin-like protein 1b (esyt1b), transcript variant X2, mRNA.

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CloneID	ODa143141
Clone ID Related Accession (Same CDS sequence)	XM_021477304.1
Accession Version	XM_021477304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	extended synaptotagmin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGACTA AATTAGACTT GGGGATTGTG AAGAAATCTA AAATAGTTGA TGAGTGGTTC 
AACCTGAAGG ACACTCAAAC AGGACGAGTT CACCTCAAAC TGGAGTGGCT GACGCTTGAA 
ACTCACACAG AAAGACTGAA AGAGGTTTTG AAGCGAAATG AAAGTGTGGT GAGTAAAGCT 
GCAGAGCCGC CCTCAGCCGC CATCTTGGCA GTGTATTTGG ACAAGGCTGA GGCACTTCCT 
ATGAAGAAAG GCAATAAGGA TCCCAATCCG ATAGTTCAAA TCTCAGTGCA AAATGCTACT 
CGTGACAGCC GGATTTGCTG GAATACTGTA AATCCCCAGT GGGAGGACGC TTTTACTTTC 
TTCATCAGAG ATCCAAACAA TCAGGACATT AGTGTACAGG TGAAGGATAA TGACCGTGTT 
CAGCTTTTGG GGAAAATGTC TATTCCTGCA TCCCGTCTCC TCTCTCATCC CGATCTGTCT 
ATGGATGAAT GGTATAATCT GGAGAATTCT GGCCCAAAGA GTCGCATTCA CATCAACACA 
GTGCTCAGGG TTCTCTGGTT GGATGAAGCC GCTGTAACTG CGTCGCTCCT CTCTAGTGGA 
CCTCTTTCAA AGAGCTCACG GCCAGAGAAA ACCACTCCCC ACTCCAGCTT CGCCACCGAG 
GGGCTGCTGC GGATCCACTT GGTCGAGGGT CAGAATCTGG TGGCTAAAGA CAATCTGATG 
GGTGGGATGG TGAAGGGGAA AAGCGACCCA TATGTCAAGA TCCAAATCGG AGGTGAAACC 
TTCAAAAGTC ACGTGATCAA GGAGAACCTC AACCCCACCT GGAATGAGAT GTATGAGGTG 
GTTTTGACGG AGCTTCCGGG TCAAGAGCTC ACGTTAGAAG TTTTTGATAA GGACATGGAT 
ATGAAAGATG ACTTTATGGG CAGGCTGAAG ATGAGTCTGA GTGACATCAT CAGCTCTCAG 
TACATTAATG AGTGGTTTTC TCTGAGCGAT GTAAAGCGTG GTCGTGTTCA CCTCGCTCTG 
GAGTGGCTTC CCACTGTAAC TAAACCAGAG AAACTACAGC AGGTGCTGCA TTTCCAGTCA 
AAGAGCTCAT TTCTGAATAA GGCAGTGCCG TCTGCAGCTC TCCTGTTTGT GTATGTGGAG 
CAAGCATATG AGCTTCCTCT CAAGAAGAGC GGGAAGGAGC CTAAAGTTGG AGCTGAGCTG 
GTCTTAGGAG GAACATCTCG AAAAACAACG GTGTGTGATC GCACCAGCAC TCCTAAATGG 
GATGAAGCGT TTTACTTTCT GGTTCGAGAC CCTTTGAATG AAGACCTTAT TGTGAAGTTG 
TCCCATAACT GGGATTTTTC CGTGGGGTCT GTGGTGATTC CTATCAAAGA GCTTCTCTCT 
GAGCCGGATC TGCTGCTGGA CCAGTGGCTG GATCTGGACG GAGCTTCACC TCAGAGTCAG 
ATTCTGCTCA GAGCTCAGCT GAAAATTCTT TGCCCTAAAA AGATGGAGAG CTCAGAGGAG 
CAGCATGAGG AGCCCAAACA TCATGAAGAG TCCAGCATCA GAAGAAAACA ACAAGAGGAG 
CTCATGCAGA AGTCTAGTAT TGAGGAGGTT CCTCCATCTC CCGTCAGCAG GACGTCCTCA 
GTTTCTGTCC CAGAAGAAGA GGAGGCACCA GAGGTCACAC AAGTCTCCAG TTCAGATGAC 
CTTCGACCCC TGCACACCAG CCCTGACCCC AGCTTTGGTA CAGAGGGTGT TCTGCGGCTC 
ATCCTGCTGG AGGCTCAAGA TCTGGTGGCG AAGGATGGTC TGATGGGTGG GATGGTGAAG 
GGGAAAAGTG ACCCGTATGT TAAGATCCAC ATCGGTGACA CAACATTTAA GAGTCACGTA 
ATCAAGGAGA ACCTCAACCC CACCTGGAAT GAGATGTATG AGCTGGTTCT GACCTCCAGC 
TCCTCTTCTG AAGTGCTCGT AGAGGTTTTC GACAAGGATA TGGACAAAGA TGACTTTTTA 
GGGAGGATGA AGATCAGTCT ACAGGAGATC ATTCAGTCTC AGATCACTGA CAGATGGTTT 
TCTCTGAGTG ACGTCAAACA TGGTCGTGTT CATTTGATTC TGGAGTGGCT GAACACAGTG 
ACAAAACCTG ACCCACTGCA AAAGGCTGTG CAGTTACAGT CAGATCATTC ATACCTGAAC 
AAAAGTGTGC CATCGGCTGC TCTGTTCTTC ATCCTCCTGG AAAGAGCTCA TAATCTGCCT 
CTGAAGAAGA GTGGAAAAGA GCCTAAAGCT GCTGCAGAGC TGGTGTTAGG AGACATCACT 
CACAAAACTA AGGTGTGTGA GAGATCCATG TCTCCTCAGT GGAGCGAAGC TTTTCACTTC 
CTGGTTCACA AACCTACTGA GGAGATTCTT ATTATCAAGC TTTCCAGTGC ATTCGAGCAG 
CCGCTGGGCT CTCTGGTTCT GCCAATCAGA GAGCTGCTTT CTAAAACAGA CCTGCTGATG 
GACCAATGGC TGAGTCTGGA TGGAGCAGCT GCTGACAGTC AGATTCTTCT CAGAGCTCAA 
CTCAAGATTT TGGACTCAAG GCTGGATTTG AGTGTTTTTC ATCGTCAGAA GCCCGTTGTT 
GAAGATCATT CAACAGGAGA AGAGCATGAA GAAAAAGGCC CAGCTACTGC TGAACATTCA 
CCCGCTGCAG TCAGTAAAGA GTCGGAGAAG AAAACACTGC CAGCAAAGCA AAGCAGGTTG 
AGTGAACTTG CAGCATCTAC TTTTCCAGTC CCTTTATCAC TGCCTGAAGA ACATCAGAAA 
CAAACAACTC ACGCCATTGA GGAGCAACAC ACACCTTCGA CACCTAAAAC ACACATGCCG 
ACTGAGGATC TCAGTAAAGC GCCTGACTCC ACTACTAATA TTGCTGAACA TAAGGAATCA 
GCCCATAAAG ATCCACAACA CCTGGATAAA GCAACAGAAC CGTCTCACAA GAGTAGTTCT 
CCCAGTGTTC CTGCTGAGGA GACTAAAGTT ACCAGTTCTA CAGACACTCG ACCCCAAAAG 
ACGAGCCACA ACTCCAACTT TGGCACCAAG GGGCTGCTGC GGCTCCACCT GCTGGAGGCT 
CAGGATCTGG TGGCGAAGGA TGGTCTGATG GGTGGGATGA TGAAGGGGAA AAGTGACCCC 
TATGTCAAGA TCCACATCGG AGACACAACA TTTAAGAGTC ACGTGATCAA GGAAAACCTC 
AACCCCACCT GGAATGAGAT GTATGAGCTG ATTCTGAGTC CTGATCCTAA TCTAGAGGTG 
AAGTTCGAGG TCTATGATAA AGATGTTGAT TCTGATGATT TTCTTGGAAG ATTTAAGCTC 
AGACTTGGAG ACATCATCAA ATCTCAGTAC AATGATGAGT GGTTTACATT GAATGATATC 
AAACATGGCC GTGTGCATCT GGTTGTGGAG TGGCTGCCCA CCGTCACCCA GCGGGACAAA 
CTGGAGCAGG TTATGCAGAT GCAGAGCTCG CAGTCCTACC AGAACAAGAG TGTGGCTTCA 
GCAGCGCTGC TCTTCATTCT GTTAGACAGA GCTCATCAGC TGCCCCTGAA GAAAAGTGGA 
AAGGAACCCA AAGCTGCAGC TGAACTTACA CTTGGAGGAA CGTCTTATAA ATCCAAGGTG 
TGTGAACGTT CATCTTCTCC TCACTGGAAT GAAACTTTTG ACTTTCTGGT TCACGACCCC 
AAAAAGGACG TGCTGGTGAT CAAGCTCTCG AGTGCCTGGG ATCAACCAAT GGGCTCTTTG 
GTTCTGCCAA TCAGAGAGCT GCTTTTAAAA CCAGATCTTC TGCTGGACCA ATGGCTGAGC 
CTGGATGGAG CTTCAGCACA GAGTCAGATT CTGCTCAGAG CTCAGCTGAA GATCTTGGAT 
TCCAAAATGG CGGCTTTAGT GGCGATGGGT TCAGGTCCGG TTTTGAGTAA CAAGCAAACG 
GCCACAACAG GACAAATCCA GCTCTCCATG TCCTTCCAGA AGAAGCTGAC GCTGCTCGTC 
CACAACTGCA GGGGTCTGGT CACATCATCT AAGGACAGCT TAGACACTTA CGTCTCCATC 
ATTCTGCTGC CAGATAAAAG CAAGGCCACC AAGAGAAAGA CCAGTGTGAA GAAGAAAACC 
CTCAATCCAG AGTTCAATGA AAAGTTTGAG TTTGATATGA GTATGGAAGA GGTTCAGAGA 
CGAGAGCTCA GTGTGTGTGT GAAAAACGCT TCCTCGTCCT TCATGAACCG AGACAAAGAC 
GTGATCGGCC AGGTGCAAAT AGACCTGGGA CACATTGATT TAATCTCTGG AGTCACACAA 
TGGTTTGATC TCAAAGAAGA GCAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332979.1
CDS	75..4364
Translation	MRTKLDLGIVKKSKIVDEWFNLKDTQTGRVHLKLEWLTLETHTERLKEVLKRNESVVSKAAEPPSAAILAVYLDKAEALPMKKGNKDPNPIVQISVQNATRDSRICWNTVNPQWEDAFTFFIRDPNNQDISVQVKDNDRVQLLGKMSIPASRLLSHPDLSMDEWYNLENSGPKSRIHINTVLRVLWLDEAAVTASLLSSGPLSKSSRPEKTTPHSSFATEGLLRIHLVEGQNLVAKDNLMGGMVKGKSDPYVKIQIGGETFKSHVIKENLNPTWNEMYEVVLTELPGQELTLEVFDKDMDMKDDFMGRLKMSLSDIISSQYINEWFSLSDVKRGRVHLALEWLPTVTKPEKLQQVLHFQSKSSFLNKAVPSAALLFVYVEQAYELPLKKSGKEPKVGAELVLGGTSRKTTVCDRTSTPKWDEAFYFLVRDPLNEDLIVKLSHNWDFSVGSVVIPIKELLSEPDLLLDQWLDLDGASPQSQILLRAQLKILCPKKMESSEEQHEEPKHHEESSIRRKQQEELMQKSSIEEVPPSPVSRTSSVSVPEEEEAPEVTQVSSSDDLRPLHTSPDPSFGTEGVLRLILLEAQDLVAKDGLMGGMVKGKSDPYVKIHIGDTTFKSHVIKENLNPTWNEMYELVLTSSSSSEVLVEVFDKDMDKDDFLGRMKISLQEIIQSQITDRWFSLSDVKHGRVHLILEWLNTVTKPDPLQKAVQLQSDHSYLNKSVPSAALFFILLERAHNLPLKKSGKEPKAAAELVLGDITHKTKVCERSMSPQWSEAFHFLVHKPTEEILIIKLSSAFEQPLGSLVLPIRELLSKTDLLMDQWLSLDGAAADSQILLRAQLKILDSRLDLSVFHRQKPVVEDHSTGEEHEEKGPATAEHSPAAVSKESEKKTLPAKQSRLSELAASTFPVPLSLPEEHQKQTTHAIEEQHTPSTPKTHMPTEDLSKAPDSTTNIAEHKESAHKDPQHLDKATEPSHKSSSPSVPAEETKVTSSTDTRPQKTSHNSNFGTKGLLRLHLLEAQDLVAKDGLMGGMMKGKSDPYVKIHIGDTTFKSHVIKENLNPTWNEMYELILSPDPNLEVKFEVYDKDVDSDDFLGRFKLRLGDIIKSQYNDEWFTLNDIKHGRVHLVVEWLPTVTQRDKLEQVMQMQSSQSYQNKSVASAALLFILLDRAHQLPLKKSGKEPKAAAELTLGGTSYKSKVCERSSSPHWNETFDFLVHDPKKDVLVIKLSSAWDQPMGSLVLPIRELLLKPDLLLDQWLSLDGASAQSQILLRAQLKILDSKMAALVAMGSGPVLSNKQTATTGQIQLSMSFQKKLTLLVHNCRGLVTSSKDSLDTYVSIILLPDKSKATKRKTSVKKKTLNPEFNEKFEFDMSMEEVQRRELSVCVKNASSSFMNRDKDVIGQVQIDLGHIDLISGVTQWFDLKEEQN

Target ORF information:

RefSeq Version	XM_021477304.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio extended synaptotagmin-like protein 1b (esyt1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477304.1

ORF Insert Sequence:

ATGAGGACTA AATTAGACTT GGGGATTGTG AAGAAATCTA AAATAGTTGA TGAGTGGTTC 
AACCTGAAGG ACACTCAAAC AGGACGAGTT CACCTCAAAC TGGAGTGGCT GACGCTTGAA 
ACTCACACAG AAAGACTGAA AGAGGTTTTG AAGCGAAATG AAAGTGTGGT GAGTAAAGCT 
GCAGAGCCGC CCTCAGCCGC CATCTTGGCA GTGTATTTGG ACAAGGCTGA GGCACTTCCT 
ATGAAGAAAG GCAATAAGGA TCCCAATCCG ATAGTTCAAA TCTCAGTGCA AAATGCTACT 
CGTGACAGCC GGATTTGCTG GAATACTGTA AATCCCCAGT GGGAGGACGC TTTTACTTTC 
TTCATCAGAG ATCCAAACAA TCAGGACATT AGTGTACAGG TGAAGGATAA TGACCGTGTT 
CAGCTTTTGG GGAAAATGTC TATTCCTGCA TCCCGTCTCC TCTCTCATCC CGATCTGTCT 
ATGGATGAAT GGTATAATCT GGAGAATTCT GGCCCAAAGA GTCGCATTCA CATCAACACA 
GTGCTCAGGG TTCTCTGGTT GGATGAAGCC GCTGTAACTG CGTCGCTCCT CTCTAGTGGA 
CCTCTTTCAA AGAGCTCACG GCCAGAGAAA ACCACTCCCC ACTCCAGCTT CGCCACCGAG 
GGGCTGCTGC GGATCCACTT GGTCGAGGGT CAGAATCTGG TGGCTAAAGA CAATCTGATG 
GGTGGGATGG TGAAGGGGAA AAGCGACCCA TATGTCAAGA TCCAAATCGG AGGTGAAACC 
TTCAAAAGTC ACGTGATCAA GGAGAACCTC AACCCCACCT GGAATGAGAT GTATGAGGTG 
GTTTTGACGG AGCTTCCGGG TCAAGAGCTC ACGTTAGAAG TTTTTGATAA GGACATGGAT 
ATGAAAGATG ACTTTATGGG CAGGCTGAAG ATGAGTCTGA GTGACATCAT CAGCTCTCAG 
TACATTAATG AGTGGTTTTC TCTGAGCGAT GTAAAGCGTG GTCGTGTTCA CCTCGCTCTG 
GAGTGGCTTC CCACTGTAAC TAAACCAGAG AAACTACAGC AGGTGCTGCA TTTCCAGTCA 
AAGAGCTCAT TTCTGAATAA GGCAGTGCCG TCTGCAGCTC TCCTGTTTGT GTATGTGGAG 
CAAGCATATG AGCTTCCTCT CAAGAAGAGC GGGAAGGAGC CTAAAGTTGG AGCTGAGCTG 
GTCTTAGGAG GAACATCTCG AAAAACAACG GTGTGTGATC GCACCAGCAC TCCTAAATGG 
GATGAAGCGT TTTACTTTCT GGTTCGAGAC CCTTTGAATG AAGACCTTAT TGTGAAGTTG 
TCCCATAACT GGGATTTTTC CGTGGGGTCT GTGGTGATTC CTATCAAAGA GCTTCTCTCT 
GAGCCGGATC TGCTGCTGGA CCAGTGGCTG GATCTGGACG GAGCTTCACC TCAGAGTCAG 
ATTCTGCTCA GAGCTCAGCT GAAAATTCTT TGCCCTAAAA AGATGGAGAG CTCAGAGGAG 
CAGCATGAGG AGCCCAAACA TCATGAAGAG TCCAGCATCA GAAGAAAACA ACAAGAGGAG 
CTCATGCAGA AGTCTAGTAT TGAGGAGGTT CCTCCATCTC CCGTCAGCAG GACGTCCTCA 
GTTTCTGTCC CAGAAGAAGA GGAGGCACCA GAGGTCACAC AAGTCTCCAG TTCAGATGAC 
CTTCGACCCC TGCACACCAG CCCTGACCCC AGCTTTGGTA CAGAGGGTGT TCTGCGGCTC 
ATCCTGCTGG AGGCTCAAGA TCTGGTGGCG AAGGATGGTC TGATGGGTGG GATGGTGAAG 
GGGAAAAGTG ACCCGTATGT TAAGATCCAC ATCGGTGACA CAACATTTAA GAGTCACGTA 
ATCAAGGAGA ACCTCAACCC CACCTGGAAT GAGATGTATG AGCTGGTTCT GACCTCCAGC 
TCCTCTTCTG AAGTGCTCGT AGAGGTTTTC GACAAGGATA TGGACAAAGA TGACTTTTTA 
GGGAGGATGA AGATCAGTCT ACAGGAGATC ATTCAGTCTC AGATCACTGA CAGATGGTTT 
TCTCTGAGTG ACGTCAAACA TGGTCGTGTT CATTTGATTC TGGAGTGGCT GAACACAGTG 
ACAAAACCTG ACCCACTGCA AAAGGCTGTG CAGTTACAGT CAGATCATTC ATACCTGAAC 
AAAAGTGTGC CATCGGCTGC TCTGTTCTTC ATCCTCCTGG AAAGAGCTCA TAATCTGCCT 
CTGAAGAAGA GTGGAAAAGA GCCTAAAGCT GCTGCAGAGC TGGTGTTAGG AGACATCACT 
CACAAAACTA AGGTGTGTGA GAGATCCATG TCTCCTCAGT GGAGCGAAGC TTTTCACTTC 
CTGGTTCACA AACCTACTGA GGAGATTCTT ATTATCAAGC TTTCCAGTGC ATTCGAGCAG 
CCGCTGGGCT CTCTGGTTCT GCCAATCAGA GAGCTGCTTT CTAAAACAGA CCTGCTGATG 
GACCAATGGC TGAGTCTGGA TGGAGCAGCT GCTGACAGTC AGATTCTTCT CAGAGCTCAA 
CTCAAGATTT TGGACTCAAG GCTGGATTTG AGTGTTTTTC ATCGTCAGAA GCCCGTTGTT 
GAAGATCATT CAACAGGAGA AGAGCATGAA GAAAAAGGCC CAGCTACTGC TGAACATTCA 
CCCGCTGCAG TCAGTAAAGA GTCGGAGAAG AAAACACTGC CAGCAAAGCA AAGCAGGTTG 
AGTGAACTTG CAGCATCTAC TTTTCCAGTC CCTTTATCAC TGCCTGAAGA ACATCAGAAA 
CAAACAACTC ACGCCATTGA GGAGCAACAC ACACCTTCGA CACCTAAAAC ACACATGCCG 
ACTGAGGATC TCAGTAAAGC GCCTGACTCC ACTACTAATA TTGCTGAACA TAAGGAATCA 
GCCCATAAAG ATCCACAACA CCTGGATAAA GCAACAGAAC CGTCTCACAA GAGTAGTTCT 
CCCAGTGTTC CTGCTGAGGA GACTAAAGTT ACCAGTTCTA CAGACACTCG ACCCCAAAAG 
ACGAGCCACA ACTCCAACTT TGGCACCAAG GGGCTGCTGC GGCTCCACCT GCTGGAGGCT 
CAGGATCTGG TGGCGAAGGA TGGTCTGATG GGTGGGATGA TGAAGGGGAA AAGTGACCCC 
TATGTCAAGA TCCACATCGG AGACACAACA TTTAAGAGTC ACGTGATCAA GGAAAACCTC 
AACCCCACCT GGAATGAGAT GTATGAGCTG ATTCTGAGTC CTGATCCTAA TCTAGAGGTG 
AAGTTCGAGG TCTATGATAA AGATGTTGAT TCTGATGATT TTCTTGGAAG ATTTAAGCTC 
AGACTTGGAG ACATCATCAA ATCTCAGTAC AATGATGAGT GGTTTACATT GAATGATATC 
AAACATGGCC GTGTGCATCT GGTTGTGGAG TGGCTGCCCA CCGTCACCCA GCGGGACAAA 
CTGGAGCAGG TTATGCAGAT GCAGAGCTCG CAGTCCTACC AGAACAAGAG TGTGGCTTCA 
GCAGCGCTGC TCTTCATTCT GTTAGACAGA GCTCATCAGC TGCCCCTGAA GAAAAGTGGA 
AAGGAACCCA AAGCTGCAGC TGAACTTACA CTTGGAGGAA CGTCTTATAA ATCCAAGGTG 
TGTGAACGTT CATCTTCTCC TCACTGGAAT GAAACTTTTG ACTTTCTGGT TCACGACCCC 
AAAAAGGACG TGCTGGTGAT CAAGCTCTCG AGTGCCTGGG ATCAACCAAT GGGCTCTTTG 
GTTCTGCCAA TCAGAGAGCT GCTTTTAAAA CCAGATCTTC TGCTGGACCA ATGGCTGAGC 
CTGGATGGAG CTTCAGCACA GAGTCAGATT CTGCTCAGAG CTCAGCTGAA GATCTTGGAT 
TCCAAAATGG CGGCTTTAGT GGCGATGGGT TCAGGTCCGG TTTTGAGTAA CAAGCAAACG 
GCCACAACAG GACAAATCCA GCTCTCCATG TCCTTCCAGA AGAAGCTGAC GCTGCTCGTC 
CACAACTGCA GGGGTCTGGT CACATCATCT AAGGACAGCT TAGACACTTA CGTCTCCATC 
ATTCTGCTGC CAGATAAAAG CAAGGCCACC AAGAGAAAGA CCAGTGTGAA GAAGAAAACC 
CTCAATCCAG AGTTCAATGA AAAGTTTGAG TTTGATATGA GTATGGAAGA GGTTCAGAGA 
CGAGAGCTCA GTGTGTGTGT GAAAAACGCT TCCTCGTCCT TCATGAACCG AGACAAAGAC 
GTGATCGGCC AGGTGCAAAT AGACCTGGGA CACATTGATT TAATCTCTGG AGTCACACAA 
TGGTTTGATC TCAAAGAAGA GCAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESYT1B ( XM_017356724.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212213.1 Danio rerio extended synaptotagmin-like protein 1b (esyt1b), transcript variant X3, mRNA.

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CloneID	ODa46083
Clone ID Related Accession (Same CDS sequence)	XM_017356724.2 , XM_017356724.1
Accession Version	XM_017356724.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	extended synaptotagmin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356724.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTATTC CTGCATCCCG TCTCCTCTCT CATCCCGATC TGTCTATGGA TGAATGGTAT 
AATCTGGAGA ATTCTGGCCC AAAGAGTCGC ATTCACATCA ACACAGTGCT CAGGGTTCTC 
TGGTTGGATG AAGCCGCTGT AACTGCGTCG CTCCTCTCTA GTGGACCTCT TTCAAAGAGC 
TCACGGCCAG AGAAAACCAC TCCCCACTCC AGCTTCGCCA CCGAGGGGCT GCTGCGGATC 
CACTTGGTCG AGGGTCAGAA TCTGGTGGCT AAAGACAATC TGATGGGTGG GATGGTGAAG 
GGGAAAAGCG ACCCATATGT CAAGATCCAA ATCGGAGGTG AAACCTTCAA AAGTCACGTG 
ATCAAGGAGA ACCTCAACCC CACCTGGAAT GAGATGTATG AGGTGGTTTT GACGGAGCTT 
CCGGGTCAAG AGCTCACGTT AGAAGTTTTT GATAAGGACA TGGATATGAA AGATGACTTT 
ATGGGCAGGC TGAAGATGAG TCTGAGTGAC ATCATCAGCT CTCAGTACAT TAATGAGTGG 
TTTTCTCTGA GCGATGTAAA GCGTGGTCGT GTTCACCTCG CTCTGGAGTG GCTTCCCACT 
GTAACTAAAC CAGAGAAACT ACAGCAGGTG CTGCATTTCC AGTCAAAGAG CTCATTTCTG 
AATAAGGCAG TGCCGTCTGC AGCTCTCCTG TTTGTGTATG TGGAGCAAGC ATATGAGCTT 
CCTCTCAAGA AGAGCGGGAA GGAGCCTAAA GTTGGAGCTG AGCTGGTCTT AGGAGGAACA 
TCTCGAAAAA CAACGGTGTG TGATCGCACC AGCACTCCTA AATGGGATGA AGCGTTTTAC 
TTTCTGGTTC GAGACCCTTT GAATGAAGAC CTTATTGTGA AGTTGTCCCA TAACTGGGAT 
TTTTCCGTGG GGTCTGTGGT GATTCCTATC AAAGAGCTTC TCTCTGAGCC GGATCTGCTG 
CTGGACCAGT GGCTGGATCT GGACGGAGCT TCACCTCAGA GTCAGATTCT GCTCAGAGCT 
CAGCTGAAAA TTCTTTGCCC TAAAAAGATG GAGAGCTCAG AGGAGCAGCA TGAGGAGCCC 
AAACATCATG AAGAGTCCAG CATCAGAAGA AAACAACAAG AGGAGCTCAT GCAGAAGTCT 
AGTATTGAGG AGGTTCCTCC ATCTCCCGTC AGCAGGACGT CCTCAGTTTC TGTCCCAGAA 
GAAGAGGAGG CACCAGAGGT CACACAAGTC TCCAGTTCAG ATGACCTTCG ACCCCTGCAC 
ACCAGCCCTG ACCCCAGCTT TGGTACAGAG GGTGTTCTGC GGCTCATCCT GCTGGAGGCT 
CAAGATCTGG TGGCGAAGGA TGGTCTGATG GGTGGGATGG TGAAGGGGAA AAGTGACCCG 
TATGTTAAGA TCCACATCGG TGACACAACA TTTAAGAGTC ACGTAATCAA GGAGAACCTC 
AACCCCACCT GGAATGAGAT GTATGAGCTG GTTCTGACCT CCAGCTCCTC TTCTGAAGTG 
CTCGTAGAGG TTTTCGACAA GGATATGGAC AAAGATGACT TTTTAGGGAG GATGAAGATC 
AGTCTACAGG AGATCATTCA GTCTCAGATC ACTGACAGAT GGTTTTCTCT GAGTGACGTC 
AAACATGGTC GTGTTCATTT GATTCTGGAG TGGCTGAACA CAGTGACAAA ACCTGACCCA 
CTGCAAAAGG CTGTGCAGTT ACAGTCAGAT CATTCATACC TGAACAAAAG TGTGCCATCG 
GCTGCTCTGT TCTTCATCCT CCTGGAAAGA GCTCATAATC TGCCTCTGAA GAAGAGTGGA 
AAAGAGCCTA AAGCTGCTGC AGAGCTGGTG TTAGGAGACA TCACTCACAA AACTAAGGTG 
TGTGAGAGAT CCATGTCTCC TCAGTGGAGC GAAGCTTTTC ACTTCCTGGT TCACAAACCT 
ACTGAGGAGA TTCTTATTAT CAAGCTTTCC AGTGCATTCG AGCAGCCGCT GGGCTCTCTG 
GTTCTGCCAA TCAGAGAGCT GCTTTCTAAA ACAGACCTGC TGATGGACCA ATGGCTGAGT 
CTGGATGGAG CAGCTGCTGA CAGTCAGATT CTTCTCAGAG CTCAACTCAA GATTTTGGAC 
TCAAGGCTGG ATTTGAGTGT TTTTCATCGT CAGAAGCCCG TTGTTGAAGA TCATTCAACA 
GGAGAAGAGC ATGAAGAAAA AGGCCCAGCT ACTGCTGAAC ATTCACCCGC TGCAGTCAGT 
AAAGAGTCGG AGAAGAAAAC ACTGCCAGCA AAGCAAAGCA GGTTGAGTGA ACTTGCAGCA 
TCTACTTTTC CAGTCCCTTT ATCACTGCCT GAAGAACATC AGAAACAAAC AACTCACGCC 
ATTGAGGAGC AACACACACC TTCGACACCT AAAACACACA TGCCGACTGA GGATCTCAGT 
AAAGCGCCTG ACTCCACTAC TAATATTGCT GAACATAAGG AATCAGCCCA TAAAGATCCA 
CAACACCTGG ATAAAGCAAC AGAACCGTCT CACAAGAGTA GTTCTCCCAG TGTTCCTGCT 
GAGGAGACTA AAGTTACCAG TTCTACAGAC ACTCGACCCC AAAAGACGAG CCACAACTCC 
AACTTTGGCA CCAAGGGGCT GCTGCGGCTC CACCTGCTGG AGGCTCAGGA TCTGGTGGCG 
AAGGATGGTC TGATGGGTGG GATGATGAAG GGGAAAAGTG ACCCCTATGT CAAGATCCAC 
ATCGGAGACA CAACATTTAA GAGTCACGTG ATCAAGGAAA ACCTCAACCC CACCTGGAAT 
GAGATGTATG AGCTGATTCT GAGTCCTGAT CCTAATCTAG AGGTGAAGTT CGAGGTCTAT 
GATAAAGATG TTGATTCTGA TGATTTTCTT GGAAGATTTA AGCTCAGACT TGGAGACATC 
ATCAAATCTC AGTACAATGA TGAGTGGTTT ACATTGAATG ATATCAAACA TGGCCGTGTG 
CATCTGGTTG TGGAGTGGCT GCCCACCGTC ACCCAGCGGG ACAAACTGGA GCAGGTTATG 
CAGATGCAGA GCTCGCAGTC CTACCAGAAC AAGAGTGTGG CTTCAGCAGC GCTGCTCTTC 
ATTCTGTTAG ACAGAGCTCA TCAGCTGCCC CTGAAGAAAA GTGGAAAGGA ACCCAAAGCT 
GCAGCTGAAC TTACACTTGG AGGAACGTCT TATAAATCCA AGGTGTGTGA ACGTTCATCT 
TCTCCTCACT GGAATGAAAC TTTTGACTTT CTGGTTCACG ACCCCAAAAA GGACGTGCTG 
GTGATCAAGC TCTCGAGTGC CTGGGATCAA CCAATGGGCT CTTTGGTTCT GCCAATCAGA 
GAGCTGCTTT TAAAACCAGA TCTTCTGCTG GACCAATGGC TGAGCCTGGA TGGAGCTTCA 
GCACAGAGTC AGATTCTGCT CAGAGCTCAG CTGAAGATCT TGGATTCCAA AATGGCGGCT 
TTAGTGGCGA TGGGTTCAGG TCCGGTTTTG AGTAACAAGC AAACGGCCAC AACAGGACAA 
ATCCAGCTCT CCATGTCCTT CCAGAAGAAG CTGACGCTGC TCGTCCACAA CTGCAGGGGT 
CTGGTCACAT CATCTAAGGA CAGCTTAGAC ACTTACGTCT CCATCATTCT GCTGCCAGAT 
AAAAGCAAGG CCACCAAGAG AAAGACCAGT GTGAAGAAGA AAACCCTCAA TCCAGAGTTC 
AATGAAAAGT TTGAGTTTGA TATGAGTATG GAAGAGGTTC AGAGACGAGA GCTCAGTGTG 
TGTGTGAAAA ACGCTTCCTC GTCCTTCATG AACCGAGACA AAGACGTGAT CGGCCAGGTG 
CAAATAGACC TGGGACACAT TGATTTAATC TCTGGAGTCA CACAATGGTT TGATCTCAAA 
GAAGAGCAGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212213.1
CDS	140..3994
Translation	MSIPASRLLSHPDLSMDEWYNLENSGPKSRIHINTVLRVLWLDEAAVTASLLSSGPLSKSSRPEKTTPHSSFATEGLLRIHLVEGQNLVAKDNLMGGMVKGKSDPYVKIQIGGETFKSHVIKENLNPTWNEMYEVVLTELPGQELTLEVFDKDMDMKDDFMGRLKMSLSDIISSQYINEWFSLSDVKRGRVHLALEWLPTVTKPEKLQQVLHFQSKSSFLNKAVPSAALLFVYVEQAYELPLKKSGKEPKVGAELVLGGTSRKTTVCDRTSTPKWDEAFYFLVRDPLNEDLIVKLSHNWDFSVGSVVIPIKELLSEPDLLLDQWLDLDGASPQSQILLRAQLKILCPKKMESSEEQHEEPKHHEESSIRRKQQEELMQKSSIEEVPPSPVSRTSSVSVPEEEEAPEVTQVSSSDDLRPLHTSPDPSFGTEGVLRLILLEAQDLVAKDGLMGGMVKGKSDPYVKIHIGDTTFKSHVIKENLNPTWNEMYELVLTSSSSSEVLVEVFDKDMDKDDFLGRMKISLQEIIQSQITDRWFSLSDVKHGRVHLILEWLNTVTKPDPLQKAVQLQSDHSYLNKSVPSAALFFILLERAHNLPLKKSGKEPKAAAELVLGDITHKTKVCERSMSPQWSEAFHFLVHKPTEEILIIKLSSAFEQPLGSLVLPIRELLSKTDLLMDQWLSLDGAAADSQILLRAQLKILDSRLDLSVFHRQKPVVEDHSTGEEHEEKGPATAEHSPAAVSKESEKKTLPAKQSRLSELAASTFPVPLSLPEEHQKQTTHAIEEQHTPSTPKTHMPTEDLSKAPDSTTNIAEHKESAHKDPQHLDKATEPSHKSSSPSVPAEETKVTSSTDTRPQKTSHNSNFGTKGLLRLHLLEAQDLVAKDGLMGGMMKGKSDPYVKIHIGDTTFKSHVIKENLNPTWNEMYELILSPDPNLEVKFEVYDKDVDSDDFLGRFKLRLGDIIKSQYNDEWFTLNDIKHGRVHLVVEWLPTVTQRDKLEQVMQMQSSQSYQNKSVASAALLFILLDRAHQLPLKKSGKEPKAAAELTLGGTSYKSKVCERSSSPHWNETFDFLVHDPKKDVLVIKLSSAWDQPMGSLVLPIRELLLKPDLLLDQWLSLDGASAQSQILLRAQLKILDSKMAALVAMGSGPVLSNKQTATTGQIQLSMSFQKKLTLLVHNCRGLVTSSKDSLDTYVSIILLPDKSKATKRKTSVKKKTLNPEFNEKFEFDMSMEEVQRRELSVCVKNASSSFMNRDKDVIGQVQIDLGHIDLISGVTQWFDLKEEQN

Target ORF information:

RefSeq Version	XM_017356724.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio extended synaptotagmin-like protein 1b (esyt1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356724.2

ORF Insert Sequence:

ATGTCTATTC CTGCATCCCG TCTCCTCTCT CATCCCGATC TGTCTATGGA TGAATGGTAT 
AATCTGGAGA ATTCTGGCCC AAAGAGTCGC ATTCACATCA ACACAGTGCT CAGGGTTCTC 
TGGTTGGATG AAGCCGCTGT AACTGCGTCG CTCCTCTCTA GTGGACCTCT TTCAAAGAGC 
TCACGGCCAG AGAAAACCAC TCCCCACTCC AGCTTCGCCA CCGAGGGGCT GCTGCGGATC 
CACTTGGTCG AGGGTCAGAA TCTGGTGGCT AAAGACAATC TGATGGGTGG GATGGTGAAG 
GGGAAAAGCG ACCCATATGT CAAGATCCAA ATCGGAGGTG AAACCTTCAA AAGTCACGTG 
ATCAAGGAGA ACCTCAACCC CACCTGGAAT GAGATGTATG AGGTGGTTTT GACGGAGCTT 
CCGGGTCAAG AGCTCACGTT AGAAGTTTTT GATAAGGACA TGGATATGAA AGATGACTTT 
ATGGGCAGGC TGAAGATGAG TCTGAGTGAC ATCATCAGCT CTCAGTACAT TAATGAGTGG 
TTTTCTCTGA GCGATGTAAA GCGTGGTCGT GTTCACCTCG CTCTGGAGTG GCTTCCCACT 
GTAACTAAAC CAGAGAAACT ACAGCAGGTG CTGCATTTCC AGTCAAAGAG CTCATTTCTG 
AATAAGGCAG TGCCGTCTGC AGCTCTCCTG TTTGTGTATG TGGAGCAAGC ATATGAGCTT 
CCTCTCAAGA AGAGCGGGAA GGAGCCTAAA GTTGGAGCTG AGCTGGTCTT AGGAGGAACA 
TCTCGAAAAA CAACGGTGTG TGATCGCACC AGCACTCCTA AATGGGATGA AGCGTTTTAC 
TTTCTGGTTC GAGACCCTTT GAATGAAGAC CTTATTGTGA AGTTGTCCCA TAACTGGGAT 
TTTTCCGTGG GGTCTGTGGT GATTCCTATC AAAGAGCTTC TCTCTGAGCC GGATCTGCTG 
CTGGACCAGT GGCTGGATCT GGACGGAGCT TCACCTCAGA GTCAGATTCT GCTCAGAGCT 
CAGCTGAAAA TTCTTTGCCC TAAAAAGATG GAGAGCTCAG AGGAGCAGCA TGAGGAGCCC 
AAACATCATG AAGAGTCCAG CATCAGAAGA AAACAACAAG AGGAGCTCAT GCAGAAGTCT 
AGTATTGAGG AGGTTCCTCC ATCTCCCGTC AGCAGGACGT CCTCAGTTTC TGTCCCAGAA 
GAAGAGGAGG CACCAGAGGT CACACAAGTC TCCAGTTCAG ATGACCTTCG ACCCCTGCAC 
ACCAGCCCTG ACCCCAGCTT TGGTACAGAG GGTGTTCTGC GGCTCATCCT GCTGGAGGCT 
CAAGATCTGG TGGCGAAGGA TGGTCTGATG GGTGGGATGG TGAAGGGGAA AAGTGACCCG 
TATGTTAAGA TCCACATCGG TGACACAACA TTTAAGAGTC ACGTAATCAA GGAGAACCTC 
AACCCCACCT GGAATGAGAT GTATGAGCTG GTTCTGACCT CCAGCTCCTC TTCTGAAGTG 
CTCGTAGAGG TTTTCGACAA GGATATGGAC AAAGATGACT TTTTAGGGAG GATGAAGATC 
AGTCTACAGG AGATCATTCA GTCTCAGATC ACTGACAGAT GGTTTTCTCT GAGTGACGTC 
AAACATGGTC GTGTTCATTT GATTCTGGAG TGGCTGAACA CAGTGACAAA ACCTGACCCA 
CTGCAAAAGG CTGTGCAGTT ACAGTCAGAT CATTCATACC TGAACAAAAG TGTGCCATCG 
GCTGCTCTGT TCTTCATCCT CCTGGAAAGA GCTCATAATC TGCCTCTGAA GAAGAGTGGA 
AAAGAGCCTA AAGCTGCTGC AGAGCTGGTG TTAGGAGACA TCACTCACAA AACTAAGGTG 
TGTGAGAGAT CCATGTCTCC TCAGTGGAGC GAAGCTTTTC ACTTCCTGGT TCACAAACCT 
ACTGAGGAGA TTCTTATTAT CAAGCTTTCC AGTGCATTCG AGCAGCCGCT GGGCTCTCTG 
GTTCTGCCAA TCAGAGAGCT GCTTTCTAAA ACAGACCTGC TGATGGACCA ATGGCTGAGT 
CTGGATGGAG CAGCTGCTGA CAGTCAGATT CTTCTCAGAG CTCAACTCAA GATTTTGGAC 
TCAAGGCTGG ATTTGAGTGT TTTTCATCGT CAGAAGCCCG TTGTTGAAGA TCATTCAACA 
GGAGAAGAGC ATGAAGAAAA AGGCCCAGCT ACTGCTGAAC ATTCACCCGC TGCAGTCAGT 
AAAGAGTCGG AGAAGAAAAC ACTGCCAGCA AAGCAAAGCA GGTTGAGTGA ACTTGCAGCA 
TCTACTTTTC CAGTCCCTTT ATCACTGCCT GAAGAACATC AGAAACAAAC AACTCACGCC 
ATTGAGGAGC AACACACACC TTCGACACCT AAAACACACA TGCCGACTGA GGATCTCAGT 
AAAGCGCCTG ACTCCACTAC TAATATTGCT GAACATAAGG AATCAGCCCA TAAAGATCCA 
CAACACCTGG ATAAAGCAAC AGAACCGTCT CACAAGAGTA GTTCTCCCAG TGTTCCTGCT 
GAGGAGACTA AAGTTACCAG TTCTACAGAC ACTCGACCCC AAAAGACGAG CCACAACTCC 
AACTTTGGCA CCAAGGGGCT GCTGCGGCTC CACCTGCTGG AGGCTCAGGA TCTGGTGGCG 
AAGGATGGTC TGATGGGTGG GATGATGAAG GGGAAAAGTG ACCCCTATGT CAAGATCCAC 
ATCGGAGACA CAACATTTAA GAGTCACGTG ATCAAGGAAA ACCTCAACCC CACCTGGAAT 
GAGATGTATG AGCTGATTCT GAGTCCTGAT CCTAATCTAG AGGTGAAGTT CGAGGTCTAT 
GATAAAGATG TTGATTCTGA TGATTTTCTT GGAAGATTTA AGCTCAGACT TGGAGACATC 
ATCAAATCTC AGTACAATGA TGAGTGGTTT ACATTGAATG ATATCAAACA TGGCCGTGTG 
CATCTGGTTG TGGAGTGGCT GCCCACCGTC ACCCAGCGGG ACAAACTGGA GCAGGTTATG 
CAGATGCAGA GCTCGCAGTC CTACCAGAAC AAGAGTGTGG CTTCAGCAGC GCTGCTCTTC 
ATTCTGTTAG ACAGAGCTCA TCAGCTGCCC CTGAAGAAAA GTGGAAAGGA ACCCAAAGCT 
GCAGCTGAAC TTACACTTGG AGGAACGTCT TATAAATCCA AGGTGTGTGA ACGTTCATCT 
TCTCCTCACT GGAATGAAAC TTTTGACTTT CTGGTTCACG ACCCCAAAAA GGACGTGCTG 
GTGATCAAGC TCTCGAGTGC CTGGGATCAA CCAATGGGCT CTTTGGTTCT GCCAATCAGA 
GAGCTGCTTT TAAAACCAGA TCTTCTGCTG GACCAATGGC TGAGCCTGGA TGGAGCTTCA 
GCACAGAGTC AGATTCTGCT CAGAGCTCAG CTGAAGATCT TGGATTCCAA AATGGCGGCT 
TTAGTGGCGA TGGGTTCAGG TCCGGTTTTG AGTAACAAGC AAACGGCCAC AACAGGACAA 
ATCCAGCTCT CCATGTCCTT CCAGAAGAAG CTGACGCTGC TCGTCCACAA CTGCAGGGGT 
CTGGTCACAT CATCTAAGGA CAGCTTAGAC ACTTACGTCT CCATCATTCT GCTGCCAGAT 
AAAAGCAAGG CCACCAAGAG AAAGACCAGT GTGAAGAAGA AAACCCTCAA TCCAGAGTTC 
AATGAAAAGT TTGAGTTTGA TATGAGTATG GAAGAGGTTC AGAGACGAGA GCTCAGTGTG 
TGTGTGAAAA ACGCTTCCTC GTCCTTCATG AACCGAGACA AAGACGTGAT CGGCCAGGTG 
CAAATAGACC TGGGACACAT TGATTTAATC TCTGGAGTCA CACAATGGTT TGATCTCAAA 
GAAGAGCAGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESYT1B ( XM_017356724.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212213.1 Danio rerio extended synaptotagmin-like protein 1b (esyt1b), transcript variant X2, mRNA.

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CloneID	ODa46083
Clone ID Related Accession (Same CDS sequence)	XM_017356724.2 , XM_017356724.1
Accession Version	XM_017356724.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	extended synaptotagmin-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTATTC CTGCATCCCG TCTCCTCTCT CATCCCGATC TGTCTATGGA TGAATGGTAT 
AATCTGGAGA ATTCTGGCCC AAAGAGTCGC ATTCACATCA ACACAGTGCT CAGGGTTCTC 
TGGTTGGATG AAGCCGCTGT AACTGCGTCG CTCCTCTCTA GTGGACCTCT TTCAAAGAGC 
TCACGGCCAG AGAAAACCAC TCCCCACTCC AGCTTCGCCA CCGAGGGGCT GCTGCGGATC 
CACTTGGTCG AGGGTCAGAA TCTGGTGGCT AAAGACAATC TGATGGGTGG GATGGTGAAG 
GGGAAAAGCG ACCCATATGT CAAGATCCAA ATCGGAGGTG AAACCTTCAA AAGTCACGTG 
ATCAAGGAGA ACCTCAACCC CACCTGGAAT GAGATGTATG AGGTGGTTTT GACGGAGCTT 
CCGGGTCAAG AGCTCACGTT AGAAGTTTTT GATAAGGACA TGGATATGAA AGATGACTTT 
ATGGGCAGGC TGAAGATGAG TCTGAGTGAC ATCATCAGCT CTCAGTACAT TAATGAGTGG 
TTTTCTCTGA GCGATGTAAA GCGTGGTCGT GTTCACCTCG CTCTGGAGTG GCTTCCCACT 
GTAACTAAAC CAGAGAAACT ACAGCAGGTG CTGCATTTCC AGTCAAAGAG CTCATTTCTG 
AATAAGGCAG TGCCGTCTGC AGCTCTCCTG TTTGTGTATG TGGAGCAAGC ATATGAGCTT 
CCTCTCAAGA AGAGCGGGAA GGAGCCTAAA GTTGGAGCTG AGCTGGTCTT AGGAGGAACA 
TCTCGAAAAA CAACGGTGTG TGATCGCACC AGCACTCCTA AATGGGATGA AGCGTTTTAC 
TTTCTGGTTC GAGACCCTTT GAATGAAGAC CTTATTGTGA AGTTGTCCCA TAACTGGGAT 
TTTTCCGTGG GGTCTGTGGT GATTCCTATC AAAGAGCTTC TCTCTGAGCC GGATCTGCTG 
CTGGACCAGT GGCTGGATCT GGACGGAGCT TCACCTCAGA GTCAGATTCT GCTCAGAGCT 
CAGCTGAAAA TTCTTTGCCC TAAAAAGATG GAGAGCTCAG AGGAGCAGCA TGAGGAGCCC 
AAACATCATG AAGAGTCCAG CATCAGAAGA AAACAACAAG AGGAGCTCAT GCAGAAGTCT 
AGTATTGAGG AGGTTCCTCC ATCTCCCGTC AGCAGGACGT CCTCAGTTTC TGTCCCAGAA 
GAAGAGGAGG CACCAGAGGT CACACAAGTC TCCAGTTCAG ATGACCTTCG ACCCCTGCAC 
ACCAGCCCTG ACCCCAGCTT TGGTACAGAG GGTGTTCTGC GGCTCATCCT GCTGGAGGCT 
CAAGATCTGG TGGCGAAGGA TGGTCTGATG GGTGGGATGG TGAAGGGGAA AAGTGACCCG 
TATGTTAAGA TCCACATCGG TGACACAACA TTTAAGAGTC ACGTAATCAA GGAGAACCTC 
AACCCCACCT GGAATGAGAT GTATGAGCTG GTTCTGACCT CCAGCTCCTC TTCTGAAGTG 
CTCGTAGAGG TTTTCGACAA GGATATGGAC AAAGATGACT TTTTAGGGAG GATGAAGATC 
AGTCTACAGG AGATCATTCA GTCTCAGATC ACTGACAGAT GGTTTTCTCT GAGTGACGTC 
AAACATGGTC GTGTTCATTT GATTCTGGAG TGGCTGAACA CAGTGACAAA ACCTGACCCA 
CTGCAAAAGG CTGTGCAGTT ACAGTCAGAT CATTCATACC TGAACAAAAG TGTGCCATCG 
GCTGCTCTGT TCTTCATCCT CCTGGAAAGA GCTCATAATC TGCCTCTGAA GAAGAGTGGA 
AAAGAGCCTA AAGCTGCTGC AGAGCTGGTG TTAGGAGACA TCACTCACAA AACTAAGGTG 
TGTGAGAGAT CCATGTCTCC TCAGTGGAGC GAAGCTTTTC ACTTCCTGGT TCACAAACCT 
ACTGAGGAGA TTCTTATTAT CAAGCTTTCC AGTGCATTCG AGCAGCCGCT GGGCTCTCTG 
GTTCTGCCAA TCAGAGAGCT GCTTTCTAAA ACAGACCTGC TGATGGACCA ATGGCTGAGT 
CTGGATGGAG CAGCTGCTGA CAGTCAGATT CTTCTCAGAG CTCAACTCAA GATTTTGGAC 
TCAAGGCTGG ATTTGAGTGT TTTTCATCGT CAGAAGCCCG TTGTTGAAGA TCATTCAACA 
GGAGAAGAGC ATGAAGAAAA AGGCCCAGCT ACTGCTGAAC ATTCACCCGC TGCAGTCAGT 
AAAGAGTCGG AGAAGAAAAC ACTGCCAGCA AAGCAAAGCA GGTTGAGTGA ACTTGCAGCA 
TCTACTTTTC CAGTCCCTTT ATCACTGCCT GAAGAACATC AGAAACAAAC AACTCACGCC 
ATTGAGGAGC AACACACACC TTCGACACCT AAAACACACA TGCCGACTGA GGATCTCAGT 
AAAGCGCCTG ACTCCACTAC TAATATTGCT GAACATAAGG AATCAGCCCA TAAAGATCCA 
CAACACCTGG ATAAAGCAAC AGAACCGTCT CACAAGAGTA GTTCTCCCAG TGTTCCTGCT 
GAGGAGACTA AAGTTACCAG TTCTACAGAC ACTCGACCCC AAAAGACGAG CCACAACTCC 
AACTTTGGCA CCAAGGGGCT GCTGCGGCTC CACCTGCTGG AGGCTCAGGA TCTGGTGGCG 
AAGGATGGTC TGATGGGTGG GATGATGAAG GGGAAAAGTG ACCCCTATGT CAAGATCCAC 
ATCGGAGACA CAACATTTAA GAGTCACGTG ATCAAGGAAA ACCTCAACCC CACCTGGAAT 
GAGATGTATG AGCTGATTCT GAGTCCTGAT CCTAATCTAG AGGTGAAGTT CGAGGTCTAT 
GATAAAGATG TTGATTCTGA TGATTTTCTT GGAAGATTTA AGCTCAGACT TGGAGACATC 
ATCAAATCTC AGTACAATGA TGAGTGGTTT ACATTGAATG ATATCAAACA TGGCCGTGTG 
CATCTGGTTG TGGAGTGGCT GCCCACCGTC ACCCAGCGGG ACAAACTGGA GCAGGTTATG 
CAGATGCAGA GCTCGCAGTC CTACCAGAAC AAGAGTGTGG CTTCAGCAGC GCTGCTCTTC 
ATTCTGTTAG ACAGAGCTCA TCAGCTGCCC CTGAAGAAAA GTGGAAAGGA ACCCAAAGCT 
GCAGCTGAAC TTACACTTGG AGGAACGTCT TATAAATCCA AGGTGTGTGA ACGTTCATCT 
TCTCCTCACT GGAATGAAAC TTTTGACTTT CTGGTTCACG ACCCCAAAAA GGACGTGCTG 
GTGATCAAGC TCTCGAGTGC CTGGGATCAA CCAATGGGCT CTTTGGTTCT GCCAATCAGA 
GAGCTGCTTT TAAAACCAGA TCTTCTGCTG GACCAATGGC TGAGCCTGGA TGGAGCTTCA 
GCACAGAGTC AGATTCTGCT CAGAGCTCAG CTGAAGATCT TGGATTCCAA AATGGCGGCT 
TTAGTGGCGA TGGGTTCAGG TCCGGTTTTG AGTAACAAGC AAACGGCCAC AACAGGACAA 
ATCCAGCTCT CCATGTCCTT CCAGAAGAAG CTGACGCTGC TCGTCCACAA CTGCAGGGGT 
CTGGTCACAT CATCTAAGGA CAGCTTAGAC ACTTACGTCT CCATCATTCT GCTGCCAGAT 
AAAAGCAAGG CCACCAAGAG AAAGACCAGT GTGAAGAAGA AAACCCTCAA TCCAGAGTTC 
AATGAAAAGT TTGAGTTTGA TATGAGTATG GAAGAGGTTC AGAGACGAGA GCTCAGTGTG 
TGTGTGAAAA ACGCTTCCTC GTCCTTCATG AACCGAGACA AAGACGTGAT CGGCCAGGTG 
CAAATAGACC TGGGACACAT TGATTTAATC TCTGGAGTCA CACAATGGTT TGATCTCAAA 
GAAGAGCAGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212213.1
CDS	150..4004
Translation	MSIPASRLLSHPDLSMDEWYNLENSGPKSRIHINTVLRVLWLDEAAVTASLLSSGPLSKSSRPEKTTPHSSFATEGLLRIHLVEGQNLVAKDNLMGGMVKGKSDPYVKIQIGGETFKSHVIKENLNPTWNEMYEVVLTELPGQELTLEVFDKDMDMKDDFMGRLKMSLSDIISSQYINEWFSLSDVKRGRVHLALEWLPTVTKPEKLQQVLHFQSKSSFLNKAVPSAALLFVYVEQAYELPLKKSGKEPKVGAELVLGGTSRKTTVCDRTSTPKWDEAFYFLVRDPLNEDLIVKLSHNWDFSVGSVVIPIKELLSEPDLLLDQWLDLDGASPQSQILLRAQLKILCPKKMESSEEQHEEPKHHEESSIRRKQQEELMQKSSIEEVPPSPVSRTSSVSVPEEEEAPEVTQVSSSDDLRPLHTSPDPSFGTEGVLRLILLEAQDLVAKDGLMGGMVKGKSDPYVKIHIGDTTFKSHVIKENLNPTWNEMYELVLTSSSSSEVLVEVFDKDMDKDDFLGRMKISLQEIIQSQITDRWFSLSDVKHGRVHLILEWLNTVTKPDPLQKAVQLQSDHSYLNKSVPSAALFFILLERAHNLPLKKSGKEPKAAAELVLGDITHKTKVCERSMSPQWSEAFHFLVHKPTEEILIIKLSSAFEQPLGSLVLPIRELLSKTDLLMDQWLSLDGAAADSQILLRAQLKILDSRLDLSVFHRQKPVVEDHSTGEEHEEKGPATAEHSPAAVSKESEKKTLPAKQSRLSELAASTFPVPLSLPEEHQKQTTHAIEEQHTPSTPKTHMPTEDLSKAPDSTTNIAEHKESAHKDPQHLDKATEPSHKSSSPSVPAEETKVTSSTDTRPQKTSHNSNFGTKGLLRLHLLEAQDLVAKDGLMGGMMKGKSDPYVKIHIGDTTFKSHVIKENLNPTWNEMYELILSPDPNLEVKFEVYDKDVDSDDFLGRFKLRLGDIIKSQYNDEWFTLNDIKHGRVHLVVEWLPTVTQRDKLEQVMQMQSSQSYQNKSVASAALLFILLDRAHQLPLKKSGKEPKAAAELTLGGTSYKSKVCERSSSPHWNETFDFLVHDPKKDVLVIKLSSAWDQPMGSLVLPIRELLLKPDLLLDQWLSLDGASAQSQILLRAQLKILDSKMAALVAMGSGPVLSNKQTATTGQIQLSMSFQKKLTLLVHNCRGLVTSSKDSLDTYVSIILLPDKSKATKRKTSVKKKTLNPEFNEKFEFDMSMEEVQRRELSVCVKNASSSFMNRDKDVIGQVQIDLGHIDLISGVTQWFDLKEEQN

Target ORF information:

RefSeq Version	XM_017356724.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio extended synaptotagmin-like protein 1b (esyt1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356724.1

ORF Insert Sequence:

ATGTCTATTC CTGCATCCCG TCTCCTCTCT CATCCCGATC TGTCTATGGA TGAATGGTAT 
AATCTGGAGA ATTCTGGCCC AAAGAGTCGC ATTCACATCA ACACAGTGCT CAGGGTTCTC 
TGGTTGGATG AAGCCGCTGT AACTGCGTCG CTCCTCTCTA GTGGACCTCT TTCAAAGAGC 
TCACGGCCAG AGAAAACCAC TCCCCACTCC AGCTTCGCCA CCGAGGGGCT GCTGCGGATC 
CACTTGGTCG AGGGTCAGAA TCTGGTGGCT AAAGACAATC TGATGGGTGG GATGGTGAAG 
GGGAAAAGCG ACCCATATGT CAAGATCCAA ATCGGAGGTG AAACCTTCAA AAGTCACGTG 
ATCAAGGAGA ACCTCAACCC CACCTGGAAT GAGATGTATG AGGTGGTTTT GACGGAGCTT 
CCGGGTCAAG AGCTCACGTT AGAAGTTTTT GATAAGGACA TGGATATGAA AGATGACTTT 
ATGGGCAGGC TGAAGATGAG TCTGAGTGAC ATCATCAGCT CTCAGTACAT TAATGAGTGG 
TTTTCTCTGA GCGATGTAAA GCGTGGTCGT GTTCACCTCG CTCTGGAGTG GCTTCCCACT 
GTAACTAAAC CAGAGAAACT ACAGCAGGTG CTGCATTTCC AGTCAAAGAG CTCATTTCTG 
AATAAGGCAG TGCCGTCTGC AGCTCTCCTG TTTGTGTATG TGGAGCAAGC ATATGAGCTT 
CCTCTCAAGA AGAGCGGGAA GGAGCCTAAA GTTGGAGCTG AGCTGGTCTT AGGAGGAACA 
TCTCGAAAAA CAACGGTGTG TGATCGCACC AGCACTCCTA AATGGGATGA AGCGTTTTAC 
TTTCTGGTTC GAGACCCTTT GAATGAAGAC CTTATTGTGA AGTTGTCCCA TAACTGGGAT 
TTTTCCGTGG GGTCTGTGGT GATTCCTATC AAAGAGCTTC TCTCTGAGCC GGATCTGCTG 
CTGGACCAGT GGCTGGATCT GGACGGAGCT TCACCTCAGA GTCAGATTCT GCTCAGAGCT 
CAGCTGAAAA TTCTTTGCCC TAAAAAGATG GAGAGCTCAG AGGAGCAGCA TGAGGAGCCC 
AAACATCATG AAGAGTCCAG CATCAGAAGA AAACAACAAG AGGAGCTCAT GCAGAAGTCT 
AGTATTGAGG AGGTTCCTCC ATCTCCCGTC AGCAGGACGT CCTCAGTTTC TGTCCCAGAA 
GAAGAGGAGG CACCAGAGGT CACACAAGTC TCCAGTTCAG ATGACCTTCG ACCCCTGCAC 
ACCAGCCCTG ACCCCAGCTT TGGTACAGAG GGTGTTCTGC GGCTCATCCT GCTGGAGGCT 
CAAGATCTGG TGGCGAAGGA TGGTCTGATG GGTGGGATGG TGAAGGGGAA AAGTGACCCG 
TATGTTAAGA TCCACATCGG TGACACAACA TTTAAGAGTC ACGTAATCAA GGAGAACCTC 
AACCCCACCT GGAATGAGAT GTATGAGCTG GTTCTGACCT CCAGCTCCTC TTCTGAAGTG 
CTCGTAGAGG TTTTCGACAA GGATATGGAC AAAGATGACT TTTTAGGGAG GATGAAGATC 
AGTCTACAGG AGATCATTCA GTCTCAGATC ACTGACAGAT GGTTTTCTCT GAGTGACGTC 
AAACATGGTC GTGTTCATTT GATTCTGGAG TGGCTGAACA CAGTGACAAA ACCTGACCCA 
CTGCAAAAGG CTGTGCAGTT ACAGTCAGAT CATTCATACC TGAACAAAAG TGTGCCATCG 
GCTGCTCTGT TCTTCATCCT CCTGGAAAGA GCTCATAATC TGCCTCTGAA GAAGAGTGGA 
AAAGAGCCTA AAGCTGCTGC AGAGCTGGTG TTAGGAGACA TCACTCACAA AACTAAGGTG 
TGTGAGAGAT CCATGTCTCC TCAGTGGAGC GAAGCTTTTC ACTTCCTGGT TCACAAACCT 
ACTGAGGAGA TTCTTATTAT CAAGCTTTCC AGTGCATTCG AGCAGCCGCT GGGCTCTCTG 
GTTCTGCCAA TCAGAGAGCT GCTTTCTAAA ACAGACCTGC TGATGGACCA ATGGCTGAGT 
CTGGATGGAG CAGCTGCTGA CAGTCAGATT CTTCTCAGAG CTCAACTCAA GATTTTGGAC 
TCAAGGCTGG ATTTGAGTGT TTTTCATCGT CAGAAGCCCG TTGTTGAAGA TCATTCAACA 
GGAGAAGAGC ATGAAGAAAA AGGCCCAGCT ACTGCTGAAC ATTCACCCGC TGCAGTCAGT 
AAAGAGTCGG AGAAGAAAAC ACTGCCAGCA AAGCAAAGCA GGTTGAGTGA ACTTGCAGCA 
TCTACTTTTC CAGTCCCTTT ATCACTGCCT GAAGAACATC AGAAACAAAC AACTCACGCC 
ATTGAGGAGC AACACACACC TTCGACACCT AAAACACACA TGCCGACTGA GGATCTCAGT 
AAAGCGCCTG ACTCCACTAC TAATATTGCT GAACATAAGG AATCAGCCCA TAAAGATCCA 
CAACACCTGG ATAAAGCAAC AGAACCGTCT CACAAGAGTA GTTCTCCCAG TGTTCCTGCT 
GAGGAGACTA AAGTTACCAG TTCTACAGAC ACTCGACCCC AAAAGACGAG CCACAACTCC 
AACTTTGGCA CCAAGGGGCT GCTGCGGCTC CACCTGCTGG AGGCTCAGGA TCTGGTGGCG 
AAGGATGGTC TGATGGGTGG GATGATGAAG GGGAAAAGTG ACCCCTATGT CAAGATCCAC 
ATCGGAGACA CAACATTTAA GAGTCACGTG ATCAAGGAAA ACCTCAACCC CACCTGGAAT 
GAGATGTATG AGCTGATTCT GAGTCCTGAT CCTAATCTAG AGGTGAAGTT CGAGGTCTAT 
GATAAAGATG TTGATTCTGA TGATTTTCTT GGAAGATTTA AGCTCAGACT TGGAGACATC 
ATCAAATCTC AGTACAATGA TGAGTGGTTT ACATTGAATG ATATCAAACA TGGCCGTGTG 
CATCTGGTTG TGGAGTGGCT GCCCACCGTC ACCCAGCGGG ACAAACTGGA GCAGGTTATG 
CAGATGCAGA GCTCGCAGTC CTACCAGAAC AAGAGTGTGG CTTCAGCAGC GCTGCTCTTC 
ATTCTGTTAG ACAGAGCTCA TCAGCTGCCC CTGAAGAAAA GTGGAAAGGA ACCCAAAGCT 
GCAGCTGAAC TTACACTTGG AGGAACGTCT TATAAATCCA AGGTGTGTGA ACGTTCATCT 
TCTCCTCACT GGAATGAAAC TTTTGACTTT CTGGTTCACG ACCCCAAAAA GGACGTGCTG 
GTGATCAAGC TCTCGAGTGC CTGGGATCAA CCAATGGGCT CTTTGGTTCT GCCAATCAGA 
GAGCTGCTTT TAAAACCAGA TCTTCTGCTG GACCAATGGC TGAGCCTGGA TGGAGCTTCA 
GCACAGAGTC AGATTCTGCT CAGAGCTCAG CTGAAGATCT TGGATTCCAA AATGGCGGCT 
TTAGTGGCGA TGGGTTCAGG TCCGGTTTTG AGTAACAAGC AAACGGCCAC AACAGGACAA 
ATCCAGCTCT CCATGTCCTT CCAGAAGAAG CTGACGCTGC TCGTCCACAA CTGCAGGGGT 
CTGGTCACAT CATCTAAGGA CAGCTTAGAC ACTTACGTCT CCATCATTCT GCTGCCAGAT 
AAAAGCAAGG CCACCAAGAG AAAGACCAGT GTGAAGAAGA AAACCCTCAA TCCAGAGTTC 
AATGAAAAGT TTGAGTTTGA TATGAGTATG GAAGAGGTTC AGAGACGAGA GCTCAGTGTG 
TGTGTGAAAA ACGCTTCCTC GTCCTTCATG AACCGAGACA AAGACGTGAT CGGCCAGGTG 
CAAATAGACC TGGGACACAT TGATTTAATC TCTGGAGTCA CACAATGGTT TGATCTCAAA 
GAAGAGCAGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ESYT1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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