ANKRD12 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKRD12
Entrez Gene ID	23253
Full Name	ankyrin repeat domain 12
Synonyms	ANCO-2,ANCO1,GAC-1,Nbla00144
General protein information	Preferred Names ankyrin repeat domain 12 Names ankyrin repeat domain-containing protein 12 ankyrin repeat-containing cofactor 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18p11.22
Summary	This gene encodes a member of the ankyrin repeats-containing cofactor family. These proteins may inhibit the transcriptional activity of nuclear receptors through the recruitment of histone deacetylases. The encoded protein interacts with p160 coactivators and also represses transcription mediated by the coactivator alteration/deficiency in activation 3 (ADA3). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011].
Disorder MIM:	610616

mRNA and Protein(s)

mRNA	Protein	Name
XM_017025667.1	XP_016881156.1	ankyrin repeat domain-containing protein 12 isoform X6
XM_017025666.1	XP_016881155.1	ankyrin repeat domain-containing protein 12 isoform X4
XM_011525639.2	XP_011523941.1	ankyrin repeat domain-containing protein 12 isoform X6
XM_017025668.1	XP_016881157.1	ankyrin repeat domain-containing protein 12 isoform X6
XM_017025669.1	XP_016881158.1	ankyrin repeat domain-containing protein 12 isoform X6
XM_005258093.3	XP_005258150.1	ankyrin repeat domain-containing protein 12 isoform X1
XM_005258093.4	XP_005258150.1	ankyrin repeat domain-containing protein 12 isoform X1
XM_005258095.3	XP_005258152.1	ankyrin repeat domain-containing protein 12 isoform X2
XM_017025664.2	XP_016881153.1	ankyrin repeat domain-containing protein 12 isoform X4
XM_017025665.2	XP_016881154.1	ankyrin repeat domain-containing protein 12 isoform X4
XM_017025666.2	XP_016881155.1	ankyrin repeat domain-containing protein 12 isoform X4
XM_017025667.2	XP_016881156.1	ankyrin repeat domain-containing protein 12 isoform X6
XM_024451109.1	XP_024306877.1	ankyrin repeat domain-containing protein 12 isoform X6
XM_017025662.1	XP_016881151.1	ankyrin repeat domain-containing protein 12 isoform X2
XM_017025663.1	XP_016881152.1	ankyrin repeat domain-containing protein 12 isoform X3
XM_017025661.1	XP_016881150.1	ankyrin repeat domain-containing protein 12 isoform X2
NM_015208.4	NP_056023.3	ankyrin repeat domain-containing protein 12 isoform 1
XM_017025661.2	XP_016881150.1	ankyrin repeat domain-containing protein 12 isoform X2
XM_011525639.3	XP_011523941.1	ankyrin repeat domain-containing protein 12 isoform X6
NM_001083625.3	NP_001077094.1	ankyrin repeat domain-containing protein 12 isoform 2
XM_017025664.1	XP_016881153.1	ankyrin repeat domain-containing protein 12 isoform X4
XM_005258092.3	XP_005258149.1	ankyrin repeat domain-containing protein 12 isoform X1
XM_017025662.2	XP_016881151.1	ankyrin repeat domain-containing protein 12 isoform X2
XM_011525638.3	XP_011523940.1	ankyrin repeat domain-containing protein 12 isoform X5
XM_017025668.2	XP_016881157.1	ankyrin repeat domain-containing protein 12 isoform X6
XM_017025670.2	XP_016881159.1	ankyrin repeat domain-containing protein 12 isoform X7
NM_015208.5	NP_056023.3	ankyrin repeat domain-containing protein 12 isoform 1
XM_011525638.2	XP_011523940.1	ankyrin repeat domain-containing protein 12 isoform X5
NM_001083625.2	NP_001077094.1	ankyrin repeat domain-containing protein 12 isoform 2
XM_017025663.2	XP_016881152.1	ankyrin repeat domain-containing protein 12 isoform X3
XM_005258096.3	XP_005258153.1	ankyrin repeat domain-containing protein 12 isoform X4
NM_001204056.1	NP_001190985.1	ankyrin repeat domain-containing protein 12 isoform 2
XM_005258095.2	XP_005258152.1	ankyrin repeat domain-containing protein 12 isoform X2
XM_005258096.2	XP_005258153.1	ankyrin repeat domain-containing protein 12 isoform X4
XM_017025665.1	XP_016881154.1	ankyrin repeat domain-containing protein 12 isoform X4
XM_017025670.1	XP_016881159.1	ankyrin repeat domain-containing protein 12 isoform X7
XM_005258092.4	XP_005258149.1	ankyrin repeat domain-containing protein 12 isoform X1
XM_024451107.1	XP_024306875.1	ankyrin repeat domain-containing protein 12 isoform X1
XM_017025669.2	XP_016881158.1	ankyrin repeat domain-containing protein 12 isoform X6

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ankrd12	NP_001101708.1
	LOC705942	XP_002808200.1
Homo sapiens (human)	ANKRD12	NP_056023.3
Pan troglodytes (chimpanzee)	ANKRD12	XP_001139573.3
Bos taurus (cattle)	ANKRD12	XP_002697790.1
Gallus gallus (chicken)	ANKRD12	XP_423591.3
Canis lupus familiaris (dog)	ANKRD12	XP_537329.2
Mus musculus (house mouse)	Ankrd12	NP_001020743.1

Gene Ontology
Bibliography

Related articles in PubMed

Clinical significance of Ankyrin repeat domain 12 expression in colorectal cancer.
Bai R, Li D, Shi Z, Fang X, Ge W, Zheng S
Journal of experimental & clinical cancer research : CR32(1)35(2013 May)

Ankyrin repeats-containing cofactors interact with ADA3 and modulate its co-activator function.
Li CW, Dinh GK, Zhang A, Chen JD
The Biochemical journal413(2)349-57(2008 Jul)

A germline-specific class of small RNAs binds mammalian Piwi proteins.
Girard A, Sachidanandam R, Hannon GJ, Carmell MA
Nature442(7099)199-202(2006 Jul)

Identification of novel ARF binding proteins by two-hybrid screening.
Tompkins V, Hagen J, Zediak VP, Quelle DE
Cell cycle (Georgetown, Tex.)5(6)641-6(2006 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study revealed that ANKRD12 mRNA were down regulated in CRC tumor tissues and low ANKRD12 expression was correlated with liver metastasis and poor survival of CRC patients.
Title: Clinical significance of Ankyrin repeat domain 12 expression in colorectal cancer.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

ADA3 is a newly identified target of the ANCO proteins, which may modulate co-activator function in a transcription-factor-specific manner
Title: Ankyrin repeats-containing cofactors interact with ADA3 and modulate its co-activator function.

The following ANKRD12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKRD12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12352	NM_015208.5 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu12352	XM_005258092.4 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu12352	XM_024451107.1 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu12352	XM_005258093.4 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu10552	NM_001083625.3 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu10552	NM_001204056.1 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu10552	XM_017025663.2 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu50996	XM_017025662.2 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu50996	XM_017025661.2 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu50996	XM_005258095.3 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu50997	XM_005258096.3 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu50997	XM_017025665.2 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu50997	XM_017025664.2 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu50997	XM_017025666.2 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu50998	XM_017025667.2 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu50998	XM_017025668.2 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu50998	XM_017025669.2 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu50998	XM_011525639.3 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu50998	XM_024451109.1 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu65805	XM_011525638.3 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu87827	XM_017025670.2 Latest version!	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu12352	XM_005258093.3	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu50997	XM_017025666.1	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu50998	XM_017025667.1	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu50996	XM_017025661.1	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu50998	XM_011525639.2	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu50997	XM_017025664.1	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu50996	XM_017025662.1	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu50998	XM_017025668.1	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu12352	NM_015208.4	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu65805	XM_011525638.2	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu10552	NM_001083625.2	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu10552	XM_017025663.1	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu50996	XM_005258095.2	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu50997	XM_005258096.2	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu50997	XM_017025665.1	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu87827	XM_017025670.1	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu12352	XM_005258092.3	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu50998	XM_017025669.1	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANKRD12 ( NM_015208.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056023.3 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant 1, mRNA.

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CloneID	OHu12352
Clone ID Related Accession (Same CDS sequence)	XM_005258093.3 , XM_005258093.4 , NM_015208.4 , XM_005258092.3 , NM_015208.5 , XM_005258092.4 , XM_024451107.1
Accession Version	NM_015208.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP230422.1, AY373757.1, AY533564.2, AP001033.6 and BM992146.1. This sequence is a reference standard in the RefSeqGene project. On Feb 12, 2011 this sequence version replaced NM_015208.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY373757.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGAAAAAC TCATTGTATC CAACGAACAA GAAGTTTTGC GAGTTCATTA CAGAGCTGCA 
AGAACATTGG CAAATCAAAC ACTGCCATTT AGTGCTTGTA CTGTTTTGCT GGATGCCGAA 
GTATACAATG TACCATTGGA CTCTCAGTCT GATGACAGTA AAACTTCTGT GAGGGATCGC 
TTTAATGCAA GACAATTCAT GTCTTGGTTA CAAGATGTGG ATGATAAATT TGACAAATTA 
AAGACCTGTC TTTTAATGAG GCAACAACAT GAAGCTGCGG CTTTAAATGC TGTCCAGAGG 
TTAGAATGGC AGCTCAAACT CCAGGAACTT GATCCTGCCA CCTATAAATC TATCAGCATT 
TACGAAATCC AGGAGTTTTA TGTTCCCCTT GTTGATGTTA ACGACGACTT TGAATTGACT 
CCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056023.3
CDS	265..6453
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	NM_015208.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015208.4

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGAAAAAC TCATTGTATC CAACGAACAA GAAGTTTTGC GAGTTCATTA CAGAGCTGCA 
AGAACATTGG CAAATCAAAC ACTGCCATTT AGTGCTTGTA CTGTTTTGCT GGATGCCGAA 
GTATACAATG TACCATTGGA CTCTCAGTCT GATGACAGTA AAACTTCTGT GAGGGATCGC 
TTTAATGCAA GACAATTCAT GTCTTGGTTA CAAGATGTGG ATGATAAATT TGACAAATTA 
AAGACCTGTC TTTTAATGAG GCAACAACAT GAAGCTGCGG CTTTAAATGC TGTCCAGAGG 
TTAGAATGGC AGCTCAAACT CCAGGAACTT GATCCTGCCA CCTATAAATC TATCAGCATT 
TACGAAATCC AGGAGTTTTA TGTTCCCCTT GTTGATGTTA ACGACGACTT TGAATTGACT 
CCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( NM_015208.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_056023.3 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant 1, mRNA.

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CloneID	OHu12352
Clone ID Related Accession (Same CDS sequence)	XM_005258093.3 , XM_005258093.4 , NM_015208.4 , XM_005258092.3 , NM_015208.5 , XM_005258092.4 , XM_024451107.1
Accession Version	NM_015208.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP230422.1, AY373757.1, AY533564.2, AP001033.6 and BM992146.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_015208.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY373757.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262126.9/ ENSP00000262126.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGAAAAAC TCATTGTATC CAACGAACAA GAAGTTTTGC GAGTTCATTA CAGAGCTGCA 
AGAACATTGG CAAATCAAAC ACTGCCATTT AGTGCTTGTA CTGTTTTGCT GGATGCCGAA 
GTATACAATG TACCATTGGA CTCTCAGTCT GATGACAGTA AAACTTCTGT GAGGGATCGC 
TTTAATGCAA GACAATTCAT GTCTTGGTTA CAAGATGTGG ATGATAAATT TGACAAATTA 
AAGACCTGTC TTTTAATGAG GCAACAACAT GAAGCTGCGG CTTTAAATGC TGTCCAGAGG 
TTAGAATGGC AGCTCAAACT CCAGGAACTT GATCCTGCCA CCTATAAATC TATCAGCATT 
TACGAAATCC AGGAGTTTTA TGTTCCCCTT GTTGATGTTA ACGACGACTT TGAATTGACT 
CCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056023.3
CDS	237..6425
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	NM_015208.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015208.5

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGAAAAAC TCATTGTATC CAACGAACAA GAAGTTTTGC GAGTTCATTA CAGAGCTGCA 
AGAACATTGG CAAATCAAAC ACTGCCATTT AGTGCTTGTA CTGTTTTGCT GGATGCCGAA 
GTATACAATG TACCATTGGA CTCTCAGTCT GATGACAGTA AAACTTCTGT GAGGGATCGC 
TTTAATGCAA GACAATTCAT GTCTTGGTTA CAAGATGTGG ATGATAAATT TGACAAATTA 
AAGACCTGTC TTTTAATGAG GCAACAACAT GAAGCTGCGG CTTTAAATGC TGTCCAGAGG 
TTAGAATGGC AGCTCAAACT CCAGGAACTT GATCCTGCCA CCTATAAATC TATCAGCATT 
TACGAAATCC AGGAGTTTTA TGTTCCCCTT GTTGATGTTA ACGACGACTT TGAATTGACT 
CCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_005258093.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258150.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X1, mRNA.

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CloneID	OHu12352
Clone ID Related Accession (Same CDS sequence)	XM_005258093.3 , XM_005258093.4 , NM_015208.4 , XM_005258092.3 , NM_015208.5 , XM_005258092.4 , XM_024451107.1
Accession Version	XM_005258093.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005258093.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGAAAAAC TCATTGTATC CAACGAACAA GAAGTTTTGC GAGTTCATTA CAGAGCTGCA 
AGAACATTGG CAAATCAAAC ACTGCCATTT AGTGCTTGTA CTGTTTTGCT GGATGCCGAA 
GTATACAATG TACCATTGGA CTCTCAGTCT GATGACAGTA AAACTTCTGT GAGGGATCGC 
TTTAATGCAA GACAATTCAT GTCTTGGTTA CAAGATGTGG ATGATAAATT TGACAAATTA 
AAGACCTGTC TTTTAATGAG GCAACAACAT GAAGCTGCGG CTTTAAATGC TGTCCAGAGG 
TTAGAATGGC AGCTCAAACT CCAGGAACTT GATCCTGCCA CCTATAAATC TATCAGCATT 
TACGAAATCC AGGAGTTTTA TGTTCCCCTT GTTGATGTTA ACGACGACTT TGAATTGACT 
CCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258150.1
CDS	382..6570
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_005258093.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005258093.3

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGAAAAAC TCATTGTATC CAACGAACAA GAAGTTTTGC GAGTTCATTA CAGAGCTGCA 
AGAACATTGG CAAATCAAAC ACTGCCATTT AGTGCTTGTA CTGTTTTGCT GGATGCCGAA 
GTATACAATG TACCATTGGA CTCTCAGTCT GATGACAGTA AAACTTCTGT GAGGGATCGC 
TTTAATGCAA GACAATTCAT GTCTTGGTTA CAAGATGTGG ATGATAAATT TGACAAATTA 
AAGACCTGTC TTTTAATGAG GCAACAACAT GAAGCTGCGG CTTTAAATGC TGTCCAGAGG 
TTAGAATGGC AGCTCAAACT CCAGGAACTT GATCCTGCCA CCTATAAATC TATCAGCATT 
TACGAAATCC AGGAGTTTTA TGTTCCCCTT GTTGATGTTA ACGACGACTT TGAATTGACT 
CCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_005258093.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258150.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X1, mRNA.

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CloneID	OHu12352
Clone ID Related Accession (Same CDS sequence)	XM_005258093.3 , XM_005258093.4 , NM_015208.4 , XM_005258092.3 , NM_015208.5 , XM_005258092.4 , XM_024451107.1
Accession Version	XM_005258093.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005258093.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGAAAAAC TCATTGTATC CAACGAACAA GAAGTTTTGC GAGTTCATTA CAGAGCTGCA 
AGAACATTGG CAAATCAAAC ACTGCCATTT AGTGCTTGTA CTGTTTTGCT GGATGCCGAA 
GTATACAATG TACCATTGGA CTCTCAGTCT GATGACAGTA AAACTTCTGT GAGGGATCGC 
TTTAATGCAA GACAATTCAT GTCTTGGTTA CAAGATGTGG ATGATAAATT TGACAAATTA 
AAGACCTGTC TTTTAATGAG GCAACAACAT GAAGCTGCGG CTTTAAATGC TGTCCAGAGG 
TTAGAATGGC AGCTCAAACT CCAGGAACTT GATCCTGCCA CCTATAAATC TATCAGCATT 
TACGAAATCC AGGAGTTTTA TGTTCCCCTT GTTGATGTTA ACGACGACTT TGAATTGACT 
CCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258150.1
CDS	382..6570
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_005258093.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005258093.4

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGAAAAAC TCATTGTATC CAACGAACAA GAAGTTTTGC GAGTTCATTA CAGAGCTGCA 
AGAACATTGG CAAATCAAAC ACTGCCATTT AGTGCTTGTA CTGTTTTGCT GGATGCCGAA 
GTATACAATG TACCATTGGA CTCTCAGTCT GATGACAGTA AAACTTCTGT GAGGGATCGC 
TTTAATGCAA GACAATTCAT GTCTTGGTTA CAAGATGTGG ATGATAAATT TGACAAATTA 
AAGACCTGTC TTTTAATGAG GCAACAACAT GAAGCTGCGG CTTTAAATGC TGTCCAGAGG 
TTAGAATGGC AGCTCAAACT CCAGGAACTT GATCCTGCCA CCTATAAATC TATCAGCATT 
TACGAAATCC AGGAGTTTTA TGTTCCCCTT GTTGATGTTA ACGACGACTT TGAATTGACT 
CCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_005258092.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258149.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X2, mRNA.

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CloneID	OHu12352
Clone ID Related Accession (Same CDS sequence)	XM_005258093.3 , XM_005258093.4 , NM_015208.4 , XM_005258092.3 , NM_015208.5 , XM_005258092.4 , XM_024451107.1
Accession Version	XM_005258092.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005258092.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGAAAAAC TCATTGTATC CAACGAACAA GAAGTTTTGC GAGTTCATTA CAGAGCTGCA 
AGAACATTGG CAAATCAAAC ACTGCCATTT AGTGCTTGTA CTGTTTTGCT GGATGCCGAA 
GTATACAATG TACCATTGGA CTCTCAGTCT GATGACAGTA AAACTTCTGT GAGGGATCGC 
TTTAATGCAA GACAATTCAT GTCTTGGTTA CAAGATGTGG ATGATAAATT TGACAAATTA 
AAGACCTGTC TTTTAATGAG GCAACAACAT GAAGCTGCGG CTTTAAATGC TGTCCAGAGG 
TTAGAATGGC AGCTCAAACT CCAGGAACTT GATCCTGCCA CCTATAAATC TATCAGCATT 
TACGAAATCC AGGAGTTTTA TGTTCCCCTT GTTGATGTTA ACGACGACTT TGAATTGACT 
CCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258149.1
CDS	398..6586
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_005258092.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005258092.3

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGAAAAAC TCATTGTATC CAACGAACAA GAAGTTTTGC GAGTTCATTA CAGAGCTGCA 
AGAACATTGG CAAATCAAAC ACTGCCATTT AGTGCTTGTA CTGTTTTGCT GGATGCCGAA 
GTATACAATG TACCATTGGA CTCTCAGTCT GATGACAGTA AAACTTCTGT GAGGGATCGC 
TTTAATGCAA GACAATTCAT GTCTTGGTTA CAAGATGTGG ATGATAAATT TGACAAATTA 
AAGACCTGTC TTTTAATGAG GCAACAACAT GAAGCTGCGG CTTTAAATGC TGTCCAGAGG 
TTAGAATGGC AGCTCAAACT CCAGGAACTT GATCCTGCCA CCTATAAATC TATCAGCATT 
TACGAAATCC AGGAGTTTTA TGTTCCCCTT GTTGATGTTA ACGACGACTT TGAATTGACT 
CCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_005258092.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258149.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X2, mRNA.

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CloneID	OHu12352
Clone ID Related Accession (Same CDS sequence)	XM_005258093.3 , XM_005258093.4 , NM_015208.4 , XM_005258092.3 , NM_015208.5 , XM_005258092.4 , XM_024451107.1
Accession Version	XM_005258092.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005258092.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGAAAAAC TCATTGTATC CAACGAACAA GAAGTTTTGC GAGTTCATTA CAGAGCTGCA 
AGAACATTGG CAAATCAAAC ACTGCCATTT AGTGCTTGTA CTGTTTTGCT GGATGCCGAA 
GTATACAATG TACCATTGGA CTCTCAGTCT GATGACAGTA AAACTTCTGT GAGGGATCGC 
TTTAATGCAA GACAATTCAT GTCTTGGTTA CAAGATGTGG ATGATAAATT TGACAAATTA 
AAGACCTGTC TTTTAATGAG GCAACAACAT GAAGCTGCGG CTTTAAATGC TGTCCAGAGG 
TTAGAATGGC AGCTCAAACT CCAGGAACTT GATCCTGCCA CCTATAAATC TATCAGCATT 
TACGAAATCC AGGAGTTTTA TGTTCCCCTT GTTGATGTTA ACGACGACTT TGAATTGACT 
CCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258149.1
CDS	398..6586
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_005258092.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005258092.4

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGAAAAAC TCATTGTATC CAACGAACAA GAAGTTTTGC GAGTTCATTA CAGAGCTGCA 
AGAACATTGG CAAATCAAAC ACTGCCATTT AGTGCTTGTA CTGTTTTGCT GGATGCCGAA 
GTATACAATG TACCATTGGA CTCTCAGTCT GATGACAGTA AAACTTCTGT GAGGGATCGC 
TTTAATGCAA GACAATTCAT GTCTTGGTTA CAAGATGTGG ATGATAAATT TGACAAATTA 
AAGACCTGTC TTTTAATGAG GCAACAACAT GAAGCTGCGG CTTTAAATGC TGTCCAGAGG 
TTAGAATGGC AGCTCAAACT CCAGGAACTT GATCCTGCCA CCTATAAATC TATCAGCATT 
TACGAAATCC AGGAGTTTTA TGTTCCCCTT GTTGATGTTA ACGACGACTT TGAATTGACT 
CCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_024451107.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306875.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X3, mRNA.

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CloneID	OHu12352
Clone ID Related Accession (Same CDS sequence)	XM_005258093.3 , XM_005258093.4 , NM_015208.4 , XM_005258092.3 , NM_015208.5 , XM_005258092.4 , XM_024451107.1
Accession Version	XM_024451107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGAAAAAC TCATTGTATC CAACGAACAA GAAGTTTTGC GAGTTCATTA CAGAGCTGCA 
AGAACATTGG CAAATCAAAC ACTGCCATTT AGTGCTTGTA CTGTTTTGCT GGATGCCGAA 
GTATACAATG TACCATTGGA CTCTCAGTCT GATGACAGTA AAACTTCTGT GAGGGATCGC 
TTTAATGCAA GACAATTCAT GTCTTGGTTA CAAGATGTGG ATGATAAATT TGACAAATTA 
AAGACCTGTC TTTTAATGAG GCAACAACAT GAAGCTGCGG CTTTAAATGC TGTCCAGAGG 
TTAGAATGGC AGCTCAAACT CCAGGAACTT GATCCTGCCA CCTATAAATC TATCAGCATT 
TACGAAATCC AGGAGTTTTA TGTTCCCCTT GTTGATGTTA ACGACGACTT TGAATTGACT 
CCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306875.1
CDS	100..6288
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_024451107.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451107.1

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGAAAAAC TCATTGTATC CAACGAACAA GAAGTTTTGC GAGTTCATTA CAGAGCTGCA 
AGAACATTGG CAAATCAAAC ACTGCCATTT AGTGCTTGTA CTGTTTTGCT GGATGCCGAA 
GTATACAATG TACCATTGGA CTCTCAGTCT GATGACAGTA AAACTTCTGT GAGGGATCGC 
TTTAATGCAA GACAATTCAT GTCTTGGTTA CAAGATGTGG ATGATAAATT TGACAAATTA 
AAGACCTGTC TTTTAATGAG GCAACAACAT GAAGCTGCGG CTTTAAATGC TGTCCAGAGG 
TTAGAATGGC AGCTCAAACT CCAGGAACTT GATCCTGCCA CCTATAAATC TATCAGCATT 
TACGAAATCC AGGAGTTTTA TGTTCCCCTT GTTGATGTTA ACGACGACTT TGAATTGACT 
CCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( NM_001083625.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077094.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant 2, mRNA.

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CloneID	OHu10552
Clone ID Related Accession (Same CDS sequence)	XM_017025663.1 , NM_001083625.3 , NM_001083625.2 , XM_017025663.2 , NM_001204056.1
Accession Version	NM_001083625.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP230422.1, AL834204.1, AY533564.2, AP001033.6 and BM992146.1. On May 31, 2019 this sequence version replaced NM_001083625.2. Transcript Variant: This variant (2) lacks an exon in the 5' coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2 and 3 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AL834204.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAGTCC AGATTCCACA CCAAATCATC CATCACAAAC AACGCCTGCC 
CAAAAGAAAA CTCCCAGTTC TTCATCTCGA CAGAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCTGCT ATTCGAGGAG ATGTGAAACA AGTTAAAGAA 
TTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ATGTTGGATA TTACGATGTT GCTAAGATAC TTATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCTGCTAG TAGTGGGCAC 
AGAGATATAG TAAAGCTGTT ACTTCGTCAC GGTGGAAATC CATTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTGGATGT AGCAGAAACA GAGGAGTTGG AGTTGCTACT AAAAAGAGAG 
GTGCCTTTAT CTGATGATGA TGAAAGTTAC ACAGATTCCG AAGAGGCTCA ATCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCT GAAACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TACTTCCCAG TAAAACACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGATGAAGA AATTAATAAG ATGATTGATG ATAGGCATAT TCTTAGGAAA 
GAACAACGAA AAGAAAATGA ACCTGAAGCA GAAAAAACTC ATTTATTTGC AAAACAGGAG 
AAAGCCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTCTTATAT 
TCAAGTACTG AAAGTTCTGA TGAAGAAGCT CTTCAGAATA AAAAGATTTC TACTTCATGT 
TCCGTCATCC CTGAAACATC AAATTCTGAT ATGCAAACCA AAAAGGAATA TGTAGTTTCA 
GGTGAACACA AACAGAAAGG CAAAGTTAAA AGAAAATTGA AAAATCAGAA TAAAAATAAA 
GAGAACCAAG AGCTAAAGCA AGAAAAGGAA GGAAAAGAAA ATACAAGAAT AACAAACTTG 
ACAGTAAATA CTGGACTAGA TTGTTCAGAA AAGACCAGAG AGGAGGGGAA CTTTAGGAAA 
TCTTTTAGCC CAAAAGATGA TACTTCATTA CATTTATTTC ATATTTCCAC TGGTAAATCT 
CCCAAACATT CTTGTGGATT AAGTGAAAAA CAGTCAACAC CACTAAAACA AGAACATACT 
AAAACATGTT TATCACCAGG AAGTTCTGAA ATGTCATTAC AGCCTGATCT TGTTCGGTAT 
GATAATACAG AATCTGAATT CTTGCCAGAA AGTTCAAGTG TAAAATCTTG TAAGCATAAG 
GAAAAAAGCA AACATCAGAA AGATTTCCAC TTAGAATTTG GTGAAAAATC AAATGCCAAA 
ATAAAGGATG AAGATCATAG TCCAACATTT GAAAATTCAG ATTGCACACT GAAAAAAATG 
GATAAAGAAG GTAAAACATT AAAAAAACAT AAATTGAAGC ATAAAGAGAG GGAAAAAGAA 
AAGCATAAAA AAGAAATTGA AGGTGAAAAG GAAAAATACA AAACTAAGGA TAGTGCCAAA 
GAACTGCAGA GGAGTGTGGA ATTTGATAGA GAATTTTGGA AAGAGAATTT TTTTAAAAGT 
GATGAAACTG AAGATCTCTT TTTAAATATG GAACATGAAT CCTTAACATT AGAAAAAAAA 
TCAAAATTGG AAAAAAACAT CAAAGATGAT AAATCAACCA AGGAAAAGCA TGTGTCAAAA 
GAGAGGAACT TTAAAGAGGA ACGAGACAAG ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG 
GAGGAAAAAA TAAAAGATCT AAAAGAAGAG AGAGAAAACA TACCCACAGA TAAAGACTCA 
GAATTTACTT CTTTGGGTAT GAGTGCCATT GAGGAATCTA TAGGGCTTCA TTTAGTGGAA 
AAGGAAATAG ACATTGAAAA ACAAGAAAAG CATATAAAGG AAAGTAAAGA AAAACCTGAG 
AAGCGATCTC AAATTAAAGA AAAGGACATT GAGAAGATGG AAAGAAAAAC CTTTGAAAAA 
GAAAAGAAGA TAAAACATGA GCATAAGTCA GAAAAAGACA AATTAGATCT TAGTGAATGT 
GTTGATAAAA TAAAAGAAAA GGACAAGCTA TATTCGCATC ACACAGAAAA ATGCCATAAA 
GAAGGTGAGA AGAGCAAAAA TACTGCTGCT ATTAAAAAAA CTGACGACAG AGAGAAAAGT 
AGAGAAAAGA TGGATAGGAA ACATGACAAA GAAAAGCCTG AAAAAGAGAG GCATCTAGCA 
GAAAGCAAAG AAAAGCACTT GATGGAGAAA AAAAATAAAC AATCAGATAA TAGTGAATAC 
AGTAAATCAG AAAAAGGCAA AAATAAAGAA AAAGACAGGG AGCTAGATAA AAAGGAAAAA 
TCTAGAGATA AAGAAAGTAT AAATATAACT AACTCCAAAC ACATACAGGA AGAAAAAAAA 
TCAAGTATAG TAGACGGTAA TAAAGCACAA CATGAAAAAC CCTTATCCCT TAAAGAAAAA 
ACAAAAGATG AACCTTTGAA AACTCCAGAT GGAAAAGAAA AAGATAAAAA AGATAAAGAT 
ATAGATAGAT ACAAAGAACG AGACAAACAT AAAGATAAAA TTCAAATAAA TAGCTTACTC 
AAACTAAAAT CTGAAGCAGA TAAGCCTAAA CCTAAGTCAT CACCAGCATC AAAAGATACC 
CGACCTAAAG AAAAGAGGTT AGTGAATGAT GATTTAATGC AGACAAGTTT TGAACGAATG 
CTAAGCCTTA AAGACCTAGA AATAGAACAG TGGCACAAAA AACATAAGGA AAAAATTAAG 
CAAAAAGAAA AGGAACGGTT GAGAAACCGA AACTGTTTAG AACTTAAAAT AAAAGATAAA 
GAAAAAACAA AGCATACACC AACTGAATCC AAAAATAAAG AACTTACTAG GTCAAAGAGT 
TCAGAAGTGA CTGATGCATA TACCAAGGAG AAACAACCTA AAGATGCTGT AAGTAACAGA 
TCACAATCTG TTGACACCAA AAATGTAATG ACTTTAGGGA AGTCATCTTT TGTTTCAGAT 
AATAGCTTAA ACAGGTCTCC TAGATCAGAA AATGAAAAGC CGGGTCTCAG CTCCAGATCT 
GTATCCATGA TTTCTGTTGC TAGTTCAGAA GATTCCTGCC ATACTACAGT GACAACCCCA 
AGGCCTCCAG TTGAGTATGA CTCTGACTTT ATGTTAGAGA GTTCAGAATC CCAAATGTCC 
TTTTCCCAGT CACCTTTTTT GTCAATTGCC AAATCTCCTG CTCTTCATGA AAGGGAATTG 
GACAGCCTGG CTGACTTGCC GGAGCGGATT AAACCACCAT ATGCAAACAG ACTTTCAACA 
TCCCATCTTA GGTCATCTTC TGTAGAAGAT GTTAAACTAA TTATAAGCGA GGGGAGACCT 
ACCATAGAAG TTCGAAGATG TAGCATGCCT TCTGTCATTT GTGAACATAC CAAACAATTC 
CAAACAATAT CAGAAGAGAG CAATCAAGGT AGCTTATTAA CTGTGCCAGG AGATACTAGT 
CCTTCTCCCA AACCTGAGGT ATTCTCAAAT GTGCCTGAAA GAGACCTTTC AAATGTATCT 
AACATACATT CCAGTTTTGC AACTTCTCCA ACTGGAGCTT CAAACAGCAA GTATGTTTCA 
GCTGATAGAA ATCTCATCAA GAATACTGCC CCAGTGAACA CTGTAATGGA CAGTCCAGTG 
CATTTAGAGC CATCTAGTCA GGTTGGTGTG ATCCAGAATA AATCATGGGA GATGCCTGTT 
GATAGACTAG AGACATTAAG CACCAGAGAC TTTATCTGCC CAAATTCTAA CATACCTGAT 
CAAGAATCCT CTCTTCAGAG TTTTTGTAAT TCTGAAAATA AGGTATTGAA AGAAAATGCT 
GATTTTTTAT CCCTGCGCCA GACTGAACTG CCAGGAAACT CTTGTGCTCA GGATCCGGCA 
TCCTTTATGC CTCCACAGCA GCCTTGCTCT TTCCCCAGCC AATCACTTTC AGATGCTGAA 
TCGATTTCTA AACATATGTC TTTGTCATAT GTTGCTAATC AAGAGCCAGG TATTTTACAA 
CAAAAAAATG CAGTTCAGAT TATTAGTTCT GCTTTAGATA CTGATAATGA ATCTACAAAA 
GATACAGAAA ATACTTTTGT CCTAGGAGAT GTTCAAAAAA CAGATGCCTT TGTCCCAGTG 
TACTCTGACA GCACTATTCA AGAAGCATCA CCAAACTTTG AGAAAGCTTA TACTTTACCT 
GTGTTACCAT CAGAAAAGGA CTTTAATGGA AGTGATGCCT CTACCCAGCT AAATACACAT 
TATGCATTTA GCAAACTAAC TTACAAGTCT TCCAGTGGCC ATGAAGTTGA GAATAGCACA 
ACTGATACTC AGGTCATTTC ACATGAAAAA GAAAACAAAC TGGAGAGTTT GGTTTTAACT 
CATTTGAGTA GGTGTGATTC TGATTTATGT GAAATGAATG CAGGGATGCC AAAAGGAAAC 
CTAAATGAAC AAGATCCAAA ACATTGTCCT GAAAGTGAAA AGTGTTTGCT TTCCATAGAA 
GATGAGGAAT CTCAACAAAG CATTTTATCA AGTCTGGAAA ACCATTCACA GCAGTCAACT 
CAACCAGAAA TGCATAAATA TGGTCAGTTA GTTAAAGTAG AATTAGAAGA AAATGCCGAA 
GATGATAAAA CTGAAAACCA AATCCCTCAA AGAATGACTA GAAACAAAGC AAATACAATG 
GCAAATCAAA GCAAACAGAT TCTTGCTAGC TGTACACTAT TATCAGAAAA AGACAGTGAA 
TCCTCATCTC CTAGAGGAAG AATAAGATTA ACTGAAGATG ACGATCCTCA AATTCACCAT 
CCACGGAAAA GGAAAGTGTC ACGTGTACCT CAGCCTGTGC AAGTGAGTCC CTCTTTACTA 
CAAGCAAAAG AGAAAACTCA GCAATCTCTG GCAGCCATTG TAGATTCTCT AAAACTAGAT 
GAGATTCAGC CATACAGTTC AGAGAGAGCA AATCCATATT TTGAATACTT GCACATAAGG 
AAAAAAATAG AAGAAAAACG CAAATTACTG TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC 
TATGACGAAT ATGTAACATT TAACGGATCA TATCTCCTGG ATGGAAACCC CTTAAGCAAG 
ATTTGTATTC CCACAATTAC ACCACCACCT TCACTGTCAG ATCCACTTAA AGAGCTTTTT 
CGACAACAGG AAGTTGTAAG GATGAAACTA CGTTTGCAAC ACAGTATTGA AAGGGAAAAA 
CTCATTGTAT CCAACGAACA AGAAGTTTTG CGAGTTCATT ACAGAGCTGC AAGAACATTG 
GCAAATCAAA CACTGCCATT TAGTGCTTGT ACTGTTTTGC TGGATGCCGA AGTATACAAT 
GTACCATTGG ACTCTCAGTC TGATGACAGT AAAACTTCTG TGAGGGATCG CTTTAATGCA 
AGACAATTCA TGTCTTGGTT ACAAGATGTG GATGATAAAT TTGACAAATT AAAGACCTGT 
CTTTTAATGA GGCAACAACA TGAAGCTGCG GCTTTAAATG CTGTCCAGAG GTTAGAATGG 
CAGCTCAAAC TCCAGGAACT TGATCCTGCC ACCTATAAAT CTATCAGCAT TTACGAAATC 
CAGGAGTTTT ATGTTCCCCT TGTTGATGTT AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077094.1
CDS	237..6356
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	NM_001083625.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083625.3

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAGTCC AGATTCCACA CCAAATCATC CATCACAAAC AACGCCTGCC 
CAAAAGAAAA CTCCCAGTTC TTCATCTCGA CAGAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCTGCT ATTCGAGGAG ATGTGAAACA AGTTAAAGAA 
TTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ATGTTGGATA TTACGATGTT GCTAAGATAC TTATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCTGCTAG TAGTGGGCAC 
AGAGATATAG TAAAGCTGTT ACTTCGTCAC GGTGGAAATC CATTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTGGATGT AGCAGAAACA GAGGAGTTGG AGTTGCTACT AAAAAGAGAG 
GTGCCTTTAT CTGATGATGA TGAAAGTTAC ACAGATTCCG AAGAGGCTCA ATCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCT GAAACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TACTTCCCAG TAAAACACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGATGAAGA AATTAATAAG ATGATTGATG ATAGGCATAT TCTTAGGAAA 
GAACAACGAA AAGAAAATGA ACCTGAAGCA GAAAAAACTC ATTTATTTGC AAAACAGGAG 
AAAGCCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTCTTATAT 
TCAAGTACTG AAAGTTCTGA TGAAGAAGCT CTTCAGAATA AAAAGATTTC TACTTCATGT 
TCCGTCATCC CTGAAACATC AAATTCTGAT ATGCAAACCA AAAAGGAATA TGTAGTTTCA 
GGTGAACACA AACAGAAAGG CAAAGTTAAA AGAAAATTGA AAAATCAGAA TAAAAATAAA 
GAGAACCAAG AGCTAAAGCA AGAAAAGGAA GGAAAAGAAA ATACAAGAAT AACAAACTTG 
ACAGTAAATA CTGGACTAGA TTGTTCAGAA AAGACCAGAG AGGAGGGGAA CTTTAGGAAA 
TCTTTTAGCC CAAAAGATGA TACTTCATTA CATTTATTTC ATATTTCCAC TGGTAAATCT 
CCCAAACATT CTTGTGGATT AAGTGAAAAA CAGTCAACAC CACTAAAACA AGAACATACT 
AAAACATGTT TATCACCAGG AAGTTCTGAA ATGTCATTAC AGCCTGATCT TGTTCGGTAT 
GATAATACAG AATCTGAATT CTTGCCAGAA AGTTCAAGTG TAAAATCTTG TAAGCATAAG 
GAAAAAAGCA AACATCAGAA AGATTTCCAC TTAGAATTTG GTGAAAAATC AAATGCCAAA 
ATAAAGGATG AAGATCATAG TCCAACATTT GAAAATTCAG ATTGCACACT GAAAAAAATG 
GATAAAGAAG GTAAAACATT AAAAAAACAT AAATTGAAGC ATAAAGAGAG GGAAAAAGAA 
AAGCATAAAA AAGAAATTGA AGGTGAAAAG GAAAAATACA AAACTAAGGA TAGTGCCAAA 
GAACTGCAGA GGAGTGTGGA ATTTGATAGA GAATTTTGGA AAGAGAATTT TTTTAAAAGT 
GATGAAACTG AAGATCTCTT TTTAAATATG GAACATGAAT CCTTAACATT AGAAAAAAAA 
TCAAAATTGG AAAAAAACAT CAAAGATGAT AAATCAACCA AGGAAAAGCA TGTGTCAAAA 
GAGAGGAACT TTAAAGAGGA ACGAGACAAG ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG 
GAGGAAAAAA TAAAAGATCT AAAAGAAGAG AGAGAAAACA TACCCACAGA TAAAGACTCA 
GAATTTACTT CTTTGGGTAT GAGTGCCATT GAGGAATCTA TAGGGCTTCA TTTAGTGGAA 
AAGGAAATAG ACATTGAAAA ACAAGAAAAG CATATAAAGG AAAGTAAAGA AAAACCTGAG 
AAGCGATCTC AAATTAAAGA AAAGGACATT GAGAAGATGG AAAGAAAAAC CTTTGAAAAA 
GAAAAGAAGA TAAAACATGA GCATAAGTCA GAAAAAGACA AATTAGATCT TAGTGAATGT 
GTTGATAAAA TAAAAGAAAA GGACAAGCTA TATTCGCATC ACACAGAAAA ATGCCATAAA 
GAAGGTGAGA AGAGCAAAAA TACTGCTGCT ATTAAAAAAA CTGACGACAG AGAGAAAAGT 
AGAGAAAAGA TGGATAGGAA ACATGACAAA GAAAAGCCTG AAAAAGAGAG GCATCTAGCA 
GAAAGCAAAG AAAAGCACTT GATGGAGAAA AAAAATAAAC AATCAGATAA TAGTGAATAC 
AGTAAATCAG AAAAAGGCAA AAATAAAGAA AAAGACAGGG AGCTAGATAA AAAGGAAAAA 
TCTAGAGATA AAGAAAGTAT AAATATAACT AACTCCAAAC ACATACAGGA AGAAAAAAAA 
TCAAGTATAG TAGACGGTAA TAAAGCACAA CATGAAAAAC CCTTATCCCT TAAAGAAAAA 
ACAAAAGATG AACCTTTGAA AACTCCAGAT GGAAAAGAAA AAGATAAAAA AGATAAAGAT 
ATAGATAGAT ACAAAGAACG AGACAAACAT AAAGATAAAA TTCAAATAAA TAGCTTACTC 
AAACTAAAAT CTGAAGCAGA TAAGCCTAAA CCTAAGTCAT CACCAGCATC AAAAGATACC 
CGACCTAAAG AAAAGAGGTT AGTGAATGAT GATTTAATGC AGACAAGTTT TGAACGAATG 
CTAAGCCTTA AAGACCTAGA AATAGAACAG TGGCACAAAA AACATAAGGA AAAAATTAAG 
CAAAAAGAAA AGGAACGGTT GAGAAACCGA AACTGTTTAG AACTTAAAAT AAAAGATAAA 
GAAAAAACAA AGCATACACC AACTGAATCC AAAAATAAAG AACTTACTAG GTCAAAGAGT 
TCAGAAGTGA CTGATGCATA TACCAAGGAG AAACAACCTA AAGATGCTGT AAGTAACAGA 
TCACAATCTG TTGACACCAA AAATGTAATG ACTTTAGGGA AGTCATCTTT TGTTTCAGAT 
AATAGCTTAA ACAGGTCTCC TAGATCAGAA AATGAAAAGC CGGGTCTCAG CTCCAGATCT 
GTATCCATGA TTTCTGTTGC TAGTTCAGAA GATTCCTGCC ATACTACAGT GACAACCCCA 
AGGCCTCCAG TTGAGTATGA CTCTGACTTT ATGTTAGAGA GTTCAGAATC CCAAATGTCC 
TTTTCCCAGT CACCTTTTTT GTCAATTGCC AAATCTCCTG CTCTTCATGA AAGGGAATTG 
GACAGCCTGG CTGACTTGCC GGAGCGGATT AAACCACCAT ATGCAAACAG ACTTTCAACA 
TCCCATCTTA GGTCATCTTC TGTAGAAGAT GTTAAACTAA TTATAAGCGA GGGGAGACCT 
ACCATAGAAG TTCGAAGATG TAGCATGCCT TCTGTCATTT GTGAACATAC CAAACAATTC 
CAAACAATAT CAGAAGAGAG CAATCAAGGT AGCTTATTAA CTGTGCCAGG AGATACTAGT 
CCTTCTCCCA AACCTGAGGT ATTCTCAAAT GTGCCTGAAA GAGACCTTTC AAATGTATCT 
AACATACATT CCAGTTTTGC AACTTCTCCA ACTGGAGCTT CAAACAGCAA GTATGTTTCA 
GCTGATAGAA ATCTCATCAA GAATACTGCC CCAGTGAACA CTGTAATGGA CAGTCCAGTG 
CATTTAGAGC CATCTAGTCA GGTTGGTGTG ATCCAGAATA AATCATGGGA GATGCCTGTT 
GATAGACTAG AGACATTAAG CACCAGAGAC TTTATCTGCC CAAATTCTAA CATACCTGAT 
CAAGAATCCT CTCTTCAGAG TTTTTGTAAT TCTGAAAATA AGGTATTGAA AGAAAATGCT 
GATTTTTTAT CCCTGCGCCA GACTGAACTG CCAGGAAACT CTTGTGCTCA GGATCCGGCA 
TCCTTTATGC CTCCACAGCA GCCTTGCTCT TTCCCCAGCC AATCACTTTC AGATGCTGAA 
TCGATTTCTA AACATATGTC TTTGTCATAT GTTGCTAATC AAGAGCCAGG TATTTTACAA 
CAAAAAAATG CAGTTCAGAT TATTAGTTCT GCTTTAGATA CTGATAATGA ATCTACAAAA 
GATACAGAAA ATACTTTTGT CCTAGGAGAT GTTCAAAAAA CAGATGCCTT TGTCCCAGTG 
TACTCTGACA GCACTATTCA AGAAGCATCA CCAAACTTTG AGAAAGCTTA TACTTTACCT 
GTGTTACCAT CAGAAAAGGA CTTTAATGGA AGTGATGCCT CTACCCAGCT AAATACACAT 
TATGCATTTA GCAAACTAAC TTACAAGTCT TCCAGTGGCC ATGAAGTTGA GAATAGCACA 
ACTGATACTC AGGTCATTTC ACATGAAAAA GAAAACAAAC TGGAGAGTTT GGTTTTAACT 
CATTTGAGTA GGTGTGATTC TGATTTATGT GAAATGAATG CAGGGATGCC AAAAGGAAAC 
CTAAATGAAC AAGATCCAAA ACATTGTCCT GAAAGTGAAA AGTGTTTGCT TTCCATAGAA 
GATGAGGAAT CTCAACAAAG CATTTTATCA AGTCTGGAAA ACCATTCACA GCAGTCAACT 
CAACCAGAAA TGCATAAATA TGGTCAGTTA GTTAAAGTAG AATTAGAAGA AAATGCCGAA 
GATGATAAAA CTGAAAACCA AATCCCTCAA AGAATGACTA GAAACAAAGC AAATACAATG 
GCAAATCAAA GCAAACAGAT TCTTGCTAGC TGTACACTAT TATCAGAAAA AGACAGTGAA 
TCCTCATCTC CTAGAGGAAG AATAAGATTA ACTGAAGATG ACGATCCTCA AATTCACCAT 
CCACGGAAAA GGAAAGTGTC ACGTGTACCT CAGCCTGTGC AAGTGAGTCC CTCTTTACTA 
CAAGCAAAAG AGAAAACTCA GCAATCTCTG GCAGCCATTG TAGATTCTCT AAAACTAGAT 
GAGATTCAGC CATACAGTTC AGAGAGAGCA AATCCATATT TTGAATACTT GCACATAAGG 
AAAAAAATAG AAGAAAAACG CAAATTACTG TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC 
TATGACGAAT ATGTAACATT TAACGGATCA TATCTCCTGG ATGGAAACCC CTTAAGCAAG 
ATTTGTATTC CCACAATTAC ACCACCACCT TCACTGTCAG ATCCACTTAA AGAGCTTTTT 
CGACAACAGG AAGTTGTAAG GATGAAACTA CGTTTGCAAC ACAGTATTGA AAGGGAAAAA 
CTCATTGTAT CCAACGAACA AGAAGTTTTG CGAGTTCATT ACAGAGCTGC AAGAACATTG 
GCAAATCAAA CACTGCCATT TAGTGCTTGT ACTGTTTTGC TGGATGCCGA AGTATACAAT 
GTACCATTGG ACTCTCAGTC TGATGACAGT AAAACTTCTG TGAGGGATCG CTTTAATGCA 
AGACAATTCA TGTCTTGGTT ACAAGATGTG GATGATAAAT TTGACAAATT AAAGACCTGT 
CTTTTAATGA GGCAACAACA TGAAGCTGCG GCTTTAAATG CTGTCCAGAG GTTAGAATGG 
CAGCTCAAAC TCCAGGAACT TGATCCTGCC ACCTATAAAT CTATCAGCAT TTACGAAATC 
CAGGAGTTTT ATGTTCCCCT TGTTGATGTT AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( NM_001083625.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077094.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant 2, mRNA.

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CloneID	OHu10552
Clone ID Related Accession (Same CDS sequence)	XM_017025663.1 , NM_001083625.3 , NM_001083625.2 , XM_017025663.2 , NM_001204056.1
Accession Version	NM_001083625.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP230422.1, AL834204.1, AY533564.2, AP001033.6 and BM992146.1. On Feb 12, 2011 this sequence version replaced NM_001083625.1. Transcript Variant: This variant (2) lacks an exon in the 5' coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2 and 3 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AL834204.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAGTCC AGATTCCACA CCAAATCATC CATCACAAAC AACGCCTGCC 
CAAAAGAAAA CTCCCAGTTC TTCATCTCGA CAGAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCTGCT ATTCGAGGAG ATGTGAAACA AGTTAAAGAA 
TTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ATGTTGGATA TTACGATGTT GCTAAGATAC TTATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCTGCTAG TAGTGGGCAC 
AGAGATATAG TAAAGCTGTT ACTTCGTCAC GGTGGAAATC CATTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTGGATGT AGCAGAAACA GAGGAGTTGG AGTTGCTACT AAAAAGAGAG 
GTGCCTTTAT CTGATGATGA TGAAAGTTAC ACAGATTCCG AAGAGGCTCA ATCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCT GAAACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TACTTCCCAG TAAAACACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGATGAAGA AATTAATAAG ATGATTGATG ATAGGCATAT TCTTAGGAAA 
GAACAACGAA AAGAAAATGA ACCTGAAGCA GAAAAAACTC ATTTATTTGC AAAACAGGAG 
AAAGCCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTCTTATAT 
TCAAGTACTG AAAGTTCTGA TGAAGAAGCT CTTCAGAATA AAAAGATTTC TACTTCATGT 
TCCGTCATCC CTGAAACATC AAATTCTGAT ATGCAAACCA AAAAGGAATA TGTAGTTTCA 
GGTGAACACA AACAGAAAGG CAAAGTTAAA AGAAAATTGA AAAATCAGAA TAAAAATAAA 
GAGAACCAAG AGCTAAAGCA AGAAAAGGAA GGAAAAGAAA ATACAAGAAT AACAAACTTG 
ACAGTAAATA CTGGACTAGA TTGTTCAGAA AAGACCAGAG AGGAGGGGAA CTTTAGGAAA 
TCTTTTAGCC CAAAAGATGA TACTTCATTA CATTTATTTC ATATTTCCAC TGGTAAATCT 
CCCAAACATT CTTGTGGATT AAGTGAAAAA CAGTCAACAC CACTAAAACA AGAACATACT 
AAAACATGTT TATCACCAGG AAGTTCTGAA ATGTCATTAC AGCCTGATCT TGTTCGGTAT 
GATAATACAG AATCTGAATT CTTGCCAGAA AGTTCAAGTG TAAAATCTTG TAAGCATAAG 
GAAAAAAGCA AACATCAGAA AGATTTCCAC TTAGAATTTG GTGAAAAATC AAATGCCAAA 
ATAAAGGATG AAGATCATAG TCCAACATTT GAAAATTCAG ATTGCACACT GAAAAAAATG 
GATAAAGAAG GTAAAACATT AAAAAAACAT AAATTGAAGC ATAAAGAGAG GGAAAAAGAA 
AAGCATAAAA AAGAAATTGA AGGTGAAAAG GAAAAATACA AAACTAAGGA TAGTGCCAAA 
GAACTGCAGA GGAGTGTGGA ATTTGATAGA GAATTTTGGA AAGAGAATTT TTTTAAAAGT 
GATGAAACTG AAGATCTCTT TTTAAATATG GAACATGAAT CCTTAACATT AGAAAAAAAA 
TCAAAATTGG AAAAAAACAT CAAAGATGAT AAATCAACCA AGGAAAAGCA TGTGTCAAAA 
GAGAGGAACT TTAAAGAGGA ACGAGACAAG ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG 
GAGGAAAAAA TAAAAGATCT AAAAGAAGAG AGAGAAAACA TACCCACAGA TAAAGACTCA 
GAATTTACTT CTTTGGGTAT GAGTGCCATT GAGGAATCTA TAGGGCTTCA TTTAGTGGAA 
AAGGAAATAG ACATTGAAAA ACAAGAAAAG CATATAAAGG AAAGTAAAGA AAAACCTGAG 
AAGCGATCTC AAATTAAAGA AAAGGACATT GAGAAGATGG AAAGAAAAAC CTTTGAAAAA 
GAAAAGAAGA TAAAACATGA GCATAAGTCA GAAAAAGACA AATTAGATCT TAGTGAATGT 
GTTGATAAAA TAAAAGAAAA GGACAAGCTA TATTCGCATC ACACAGAAAA ATGCCATAAA 
GAAGGTGAGA AGAGCAAAAA TACTGCTGCT ATTAAAAAAA CTGACGACAG AGAGAAAAGT 
AGAGAAAAGA TGGATAGGAA ACATGACAAA GAAAAGCCTG AAAAAGAGAG GCATCTAGCA 
GAAAGCAAAG AAAAGCACTT GATGGAGAAA AAAAATAAAC AATCAGATAA TAGTGAATAC 
AGTAAATCAG AAAAAGGCAA AAATAAAGAA AAAGACAGGG AGCTAGATAA AAAGGAAAAA 
TCTAGAGATA AAGAAAGTAT AAATATAACT AACTCCAAAC ACATACAGGA AGAAAAAAAA 
TCAAGTATAG TAGACGGTAA TAAAGCACAA CATGAAAAAC CCTTATCCCT TAAAGAAAAA 
ACAAAAGATG AACCTTTGAA AACTCCAGAT GGAAAAGAAA AAGATAAAAA AGATAAAGAT 
ATAGATAGAT ACAAAGAACG AGACAAACAT AAAGATAAAA TTCAAATAAA TAGCTTACTC 
AAACTAAAAT CTGAAGCAGA TAAGCCTAAA CCTAAGTCAT CACCAGCATC AAAAGATACC 
CGACCTAAAG AAAAGAGGTT AGTGAATGAT GATTTAATGC AGACAAGTTT TGAACGAATG 
CTAAGCCTTA AAGACCTAGA AATAGAACAG TGGCACAAAA AACATAAGGA AAAAATTAAG 
CAAAAAGAAA AGGAACGGTT GAGAAACCGA AACTGTTTAG AACTTAAAAT AAAAGATAAA 
GAAAAAACAA AGCATACACC AACTGAATCC AAAAATAAAG AACTTACTAG GTCAAAGAGT 
TCAGAAGTGA CTGATGCATA TACCAAGGAG AAACAACCTA AAGATGCTGT AAGTAACAGA 
TCACAATCTG TTGACACCAA AAATGTAATG ACTTTAGGGA AGTCATCTTT TGTTTCAGAT 
AATAGCTTAA ACAGGTCTCC TAGATCAGAA AATGAAAAGC CGGGTCTCAG CTCCAGATCT 
GTATCCATGA TTTCTGTTGC TAGTTCAGAA GATTCCTGCC ATACTACAGT GACAACCCCA 
AGGCCTCCAG TTGAGTATGA CTCTGACTTT ATGTTAGAGA GTTCAGAATC CCAAATGTCC 
TTTTCCCAGT CACCTTTTTT GTCAATTGCC AAATCTCCTG CTCTTCATGA AAGGGAATTG 
GACAGCCTGG CTGACTTGCC GGAGCGGATT AAACCACCAT ATGCAAACAG ACTTTCAACA 
TCCCATCTTA GGTCATCTTC TGTAGAAGAT GTTAAACTAA TTATAAGCGA GGGGAGACCT 
ACCATAGAAG TTCGAAGATG TAGCATGCCT TCTGTCATTT GTGAACATAC CAAACAATTC 
CAAACAATAT CAGAAGAGAG CAATCAAGGT AGCTTATTAA CTGTGCCAGG AGATACTAGT 
CCTTCTCCCA AACCTGAGGT ATTCTCAAAT GTGCCTGAAA GAGACCTTTC AAATGTATCT 
AACATACATT CCAGTTTTGC AACTTCTCCA ACTGGAGCTT CAAACAGCAA GTATGTTTCA 
GCTGATAGAA ATCTCATCAA GAATACTGCC CCAGTGAACA CTGTAATGGA CAGTCCAGTG 
CATTTAGAGC CATCTAGTCA GGTTGGTGTG ATCCAGAATA AATCATGGGA GATGCCTGTT 
GATAGACTAG AGACATTAAG CACCAGAGAC TTTATCTGCC CAAATTCTAA CATACCTGAT 
CAAGAATCCT CTCTTCAGAG TTTTTGTAAT TCTGAAAATA AGGTATTGAA AGAAAATGCT 
GATTTTTTAT CCCTGCGCCA GACTGAACTG CCAGGAAACT CTTGTGCTCA GGATCCGGCA 
TCCTTTATGC CTCCACAGCA GCCTTGCTCT TTCCCCAGCC AATCACTTTC AGATGCTGAA 
TCGATTTCTA AACATATGTC TTTGTCATAT GTTGCTAATC AAGAGCCAGG TATTTTACAA 
CAAAAAAATG CAGTTCAGAT TATTAGTTCT GCTTTAGATA CTGATAATGA ATCTACAAAA 
GATACAGAAA ATACTTTTGT CCTAGGAGAT GTTCAAAAAA CAGATGCCTT TGTCCCAGTG 
TACTCTGACA GCACTATTCA AGAAGCATCA CCAAACTTTG AGAAAGCTTA TACTTTACCT 
GTGTTACCAT CAGAAAAGGA CTTTAATGGA AGTGATGCCT CTACCCAGCT AAATACACAT 
TATGCATTTA GCAAACTAAC TTACAAGTCT TCCAGTGGCC ATGAAGTTGA GAATAGCACA 
ACTGATACTC AGGTCATTTC ACATGAAAAA GAAAACAAAC TGGAGAGTTT GGTTTTAACT 
CATTTGAGTA GGTGTGATTC TGATTTATGT GAAATGAATG CAGGGATGCC AAAAGGAAAC 
CTAAATGAAC AAGATCCAAA ACATTGTCCT GAAAGTGAAA AGTGTTTGCT TTCCATAGAA 
GATGAGGAAT CTCAACAAAG CATTTTATCA AGTCTGGAAA ACCATTCACA GCAGTCAACT 
CAACCAGAAA TGCATAAATA TGGTCAGTTA GTTAAAGTAG AATTAGAAGA AAATGCCGAA 
GATGATAAAA CTGAAAACCA AATCCCTCAA AGAATGACTA GAAACAAAGC AAATACAATG 
GCAAATCAAA GCAAACAGAT TCTTGCTAGC TGTACACTAT TATCAGAAAA AGACAGTGAA 
TCCTCATCTC CTAGAGGAAG AATAAGATTA ACTGAAGATG ACGATCCTCA AATTCACCAT 
CCACGGAAAA GGAAAGTGTC ACGTGTACCT CAGCCTGTGC AAGTGAGTCC CTCTTTACTA 
CAAGCAAAAG AGAAAACTCA GCAATCTCTG GCAGCCATTG TAGATTCTCT AAAACTAGAT 
GAGATTCAGC CATACAGTTC AGAGAGAGCA AATCCATATT TTGAATACTT GCACATAAGG 
AAAAAAATAG AAGAAAAACG CAAATTACTG TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC 
TATGACGAAT ATGTAACATT TAACGGATCA TATCTCCTGG ATGGAAACCC CTTAAGCAAG 
ATTTGTATTC CCACAATTAC ACCACCACCT TCACTGTCAG ATCCACTTAA AGAGCTTTTT 
CGACAACAGG AAGTTGTAAG GATGAAACTA CGTTTGCAAC ACAGTATTGA AAGGGAAAAA 
CTCATTGTAT CCAACGAACA AGAAGTTTTG CGAGTTCATT ACAGAGCTGC AAGAACATTG 
GCAAATCAAA CACTGCCATT TAGTGCTTGT ACTGTTTTGC TGGATGCCGA AGTATACAAT 
GTACCATTGG ACTCTCAGTC TGATGACAGT AAAACTTCTG TGAGGGATCG CTTTAATGCA 
AGACAATTCA TGTCTTGGTT ACAAGATGTG GATGATAAAT TTGACAAATT AAAGACCTGT 
CTTTTAATGA GGCAACAACA TGAAGCTGCG GCTTTAAATG CTGTCCAGAG GTTAGAATGG 
CAGCTCAAAC TCCAGGAACT TGATCCTGCC ACCTATAAAT CTATCAGCAT TTACGAAATC 
CAGGAGTTTT ATGTTCCCCT TGTTGATGTT AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077094.1
CDS	265..6384
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	NM_001083625.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083625.2

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAGTCC AGATTCCACA CCAAATCATC CATCACAAAC AACGCCTGCC 
CAAAAGAAAA CTCCCAGTTC TTCATCTCGA CAGAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCTGCT ATTCGAGGAG ATGTGAAACA AGTTAAAGAA 
TTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ATGTTGGATA TTACGATGTT GCTAAGATAC TTATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCTGCTAG TAGTGGGCAC 
AGAGATATAG TAAAGCTGTT ACTTCGTCAC GGTGGAAATC CATTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTGGATGT AGCAGAAACA GAGGAGTTGG AGTTGCTACT AAAAAGAGAG 
GTGCCTTTAT CTGATGATGA TGAAAGTTAC ACAGATTCCG AAGAGGCTCA ATCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCT GAAACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TACTTCCCAG TAAAACACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGATGAAGA AATTAATAAG ATGATTGATG ATAGGCATAT TCTTAGGAAA 
GAACAACGAA AAGAAAATGA ACCTGAAGCA GAAAAAACTC ATTTATTTGC AAAACAGGAG 
AAAGCCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTCTTATAT 
TCAAGTACTG AAAGTTCTGA TGAAGAAGCT CTTCAGAATA AAAAGATTTC TACTTCATGT 
TCCGTCATCC CTGAAACATC AAATTCTGAT ATGCAAACCA AAAAGGAATA TGTAGTTTCA 
GGTGAACACA AACAGAAAGG CAAAGTTAAA AGAAAATTGA AAAATCAGAA TAAAAATAAA 
GAGAACCAAG AGCTAAAGCA AGAAAAGGAA GGAAAAGAAA ATACAAGAAT AACAAACTTG 
ACAGTAAATA CTGGACTAGA TTGTTCAGAA AAGACCAGAG AGGAGGGGAA CTTTAGGAAA 
TCTTTTAGCC CAAAAGATGA TACTTCATTA CATTTATTTC ATATTTCCAC TGGTAAATCT 
CCCAAACATT CTTGTGGATT AAGTGAAAAA CAGTCAACAC CACTAAAACA AGAACATACT 
AAAACATGTT TATCACCAGG AAGTTCTGAA ATGTCATTAC AGCCTGATCT TGTTCGGTAT 
GATAATACAG AATCTGAATT CTTGCCAGAA AGTTCAAGTG TAAAATCTTG TAAGCATAAG 
GAAAAAAGCA AACATCAGAA AGATTTCCAC TTAGAATTTG GTGAAAAATC AAATGCCAAA 
ATAAAGGATG AAGATCATAG TCCAACATTT GAAAATTCAG ATTGCACACT GAAAAAAATG 
GATAAAGAAG GTAAAACATT AAAAAAACAT AAATTGAAGC ATAAAGAGAG GGAAAAAGAA 
AAGCATAAAA AAGAAATTGA AGGTGAAAAG GAAAAATACA AAACTAAGGA TAGTGCCAAA 
GAACTGCAGA GGAGTGTGGA ATTTGATAGA GAATTTTGGA AAGAGAATTT TTTTAAAAGT 
GATGAAACTG AAGATCTCTT TTTAAATATG GAACATGAAT CCTTAACATT AGAAAAAAAA 
TCAAAATTGG AAAAAAACAT CAAAGATGAT AAATCAACCA AGGAAAAGCA TGTGTCAAAA 
GAGAGGAACT TTAAAGAGGA ACGAGACAAG ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG 
GAGGAAAAAA TAAAAGATCT AAAAGAAGAG AGAGAAAACA TACCCACAGA TAAAGACTCA 
GAATTTACTT CTTTGGGTAT GAGTGCCATT GAGGAATCTA TAGGGCTTCA TTTAGTGGAA 
AAGGAAATAG ACATTGAAAA ACAAGAAAAG CATATAAAGG AAAGTAAAGA AAAACCTGAG 
AAGCGATCTC AAATTAAAGA AAAGGACATT GAGAAGATGG AAAGAAAAAC CTTTGAAAAA 
GAAAAGAAGA TAAAACATGA GCATAAGTCA GAAAAAGACA AATTAGATCT TAGTGAATGT 
GTTGATAAAA TAAAAGAAAA GGACAAGCTA TATTCGCATC ACACAGAAAA ATGCCATAAA 
GAAGGTGAGA AGAGCAAAAA TACTGCTGCT ATTAAAAAAA CTGACGACAG AGAGAAAAGT 
AGAGAAAAGA TGGATAGGAA ACATGACAAA GAAAAGCCTG AAAAAGAGAG GCATCTAGCA 
GAAAGCAAAG AAAAGCACTT GATGGAGAAA AAAAATAAAC AATCAGATAA TAGTGAATAC 
AGTAAATCAG AAAAAGGCAA AAATAAAGAA AAAGACAGGG AGCTAGATAA AAAGGAAAAA 
TCTAGAGATA AAGAAAGTAT AAATATAACT AACTCCAAAC ACATACAGGA AGAAAAAAAA 
TCAAGTATAG TAGACGGTAA TAAAGCACAA CATGAAAAAC CCTTATCCCT TAAAGAAAAA 
ACAAAAGATG AACCTTTGAA AACTCCAGAT GGAAAAGAAA AAGATAAAAA AGATAAAGAT 
ATAGATAGAT ACAAAGAACG AGACAAACAT AAAGATAAAA TTCAAATAAA TAGCTTACTC 
AAACTAAAAT CTGAAGCAGA TAAGCCTAAA CCTAAGTCAT CACCAGCATC AAAAGATACC 
CGACCTAAAG AAAAGAGGTT AGTGAATGAT GATTTAATGC AGACAAGTTT TGAACGAATG 
CTAAGCCTTA AAGACCTAGA AATAGAACAG TGGCACAAAA AACATAAGGA AAAAATTAAG 
CAAAAAGAAA AGGAACGGTT GAGAAACCGA AACTGTTTAG AACTTAAAAT AAAAGATAAA 
GAAAAAACAA AGCATACACC AACTGAATCC AAAAATAAAG AACTTACTAG GTCAAAGAGT 
TCAGAAGTGA CTGATGCATA TACCAAGGAG AAACAACCTA AAGATGCTGT AAGTAACAGA 
TCACAATCTG TTGACACCAA AAATGTAATG ACTTTAGGGA AGTCATCTTT TGTTTCAGAT 
AATAGCTTAA ACAGGTCTCC TAGATCAGAA AATGAAAAGC CGGGTCTCAG CTCCAGATCT 
GTATCCATGA TTTCTGTTGC TAGTTCAGAA GATTCCTGCC ATACTACAGT GACAACCCCA 
AGGCCTCCAG TTGAGTATGA CTCTGACTTT ATGTTAGAGA GTTCAGAATC CCAAATGTCC 
TTTTCCCAGT CACCTTTTTT GTCAATTGCC AAATCTCCTG CTCTTCATGA AAGGGAATTG 
GACAGCCTGG CTGACTTGCC GGAGCGGATT AAACCACCAT ATGCAAACAG ACTTTCAACA 
TCCCATCTTA GGTCATCTTC TGTAGAAGAT GTTAAACTAA TTATAAGCGA GGGGAGACCT 
ACCATAGAAG TTCGAAGATG TAGCATGCCT TCTGTCATTT GTGAACATAC CAAACAATTC 
CAAACAATAT CAGAAGAGAG CAATCAAGGT AGCTTATTAA CTGTGCCAGG AGATACTAGT 
CCTTCTCCCA AACCTGAGGT ATTCTCAAAT GTGCCTGAAA GAGACCTTTC AAATGTATCT 
AACATACATT CCAGTTTTGC AACTTCTCCA ACTGGAGCTT CAAACAGCAA GTATGTTTCA 
GCTGATAGAA ATCTCATCAA GAATACTGCC CCAGTGAACA CTGTAATGGA CAGTCCAGTG 
CATTTAGAGC CATCTAGTCA GGTTGGTGTG ATCCAGAATA AATCATGGGA GATGCCTGTT 
GATAGACTAG AGACATTAAG CACCAGAGAC TTTATCTGCC CAAATTCTAA CATACCTGAT 
CAAGAATCCT CTCTTCAGAG TTTTTGTAAT TCTGAAAATA AGGTATTGAA AGAAAATGCT 
GATTTTTTAT CCCTGCGCCA GACTGAACTG CCAGGAAACT CTTGTGCTCA GGATCCGGCA 
TCCTTTATGC CTCCACAGCA GCCTTGCTCT TTCCCCAGCC AATCACTTTC AGATGCTGAA 
TCGATTTCTA AACATATGTC TTTGTCATAT GTTGCTAATC AAGAGCCAGG TATTTTACAA 
CAAAAAAATG CAGTTCAGAT TATTAGTTCT GCTTTAGATA CTGATAATGA ATCTACAAAA 
GATACAGAAA ATACTTTTGT CCTAGGAGAT GTTCAAAAAA CAGATGCCTT TGTCCCAGTG 
TACTCTGACA GCACTATTCA AGAAGCATCA CCAAACTTTG AGAAAGCTTA TACTTTACCT 
GTGTTACCAT CAGAAAAGGA CTTTAATGGA AGTGATGCCT CTACCCAGCT AAATACACAT 
TATGCATTTA GCAAACTAAC TTACAAGTCT TCCAGTGGCC ATGAAGTTGA GAATAGCACA 
ACTGATACTC AGGTCATTTC ACATGAAAAA GAAAACAAAC TGGAGAGTTT GGTTTTAACT 
CATTTGAGTA GGTGTGATTC TGATTTATGT GAAATGAATG CAGGGATGCC AAAAGGAAAC 
CTAAATGAAC AAGATCCAAA ACATTGTCCT GAAAGTGAAA AGTGTTTGCT TTCCATAGAA 
GATGAGGAAT CTCAACAAAG CATTTTATCA AGTCTGGAAA ACCATTCACA GCAGTCAACT 
CAACCAGAAA TGCATAAATA TGGTCAGTTA GTTAAAGTAG AATTAGAAGA AAATGCCGAA 
GATGATAAAA CTGAAAACCA AATCCCTCAA AGAATGACTA GAAACAAAGC AAATACAATG 
GCAAATCAAA GCAAACAGAT TCTTGCTAGC TGTACACTAT TATCAGAAAA AGACAGTGAA 
TCCTCATCTC CTAGAGGAAG AATAAGATTA ACTGAAGATG ACGATCCTCA AATTCACCAT 
CCACGGAAAA GGAAAGTGTC ACGTGTACCT CAGCCTGTGC AAGTGAGTCC CTCTTTACTA 
CAAGCAAAAG AGAAAACTCA GCAATCTCTG GCAGCCATTG TAGATTCTCT AAAACTAGAT 
GAGATTCAGC CATACAGTTC AGAGAGAGCA AATCCATATT TTGAATACTT GCACATAAGG 
AAAAAAATAG AAGAAAAACG CAAATTACTG TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC 
TATGACGAAT ATGTAACATT TAACGGATCA TATCTCCTGG ATGGAAACCC CTTAAGCAAG 
ATTTGTATTC CCACAATTAC ACCACCACCT TCACTGTCAG ATCCACTTAA AGAGCTTTTT 
CGACAACAGG AAGTTGTAAG GATGAAACTA CGTTTGCAAC ACAGTATTGA AAGGGAAAAA 
CTCATTGTAT CCAACGAACA AGAAGTTTTG CGAGTTCATT ACAGAGCTGC AAGAACATTG 
GCAAATCAAA CACTGCCATT TAGTGCTTGT ACTGTTTTGC TGGATGCCGA AGTATACAAT 
GTACCATTGG ACTCTCAGTC TGATGACAGT AAAACTTCTG TGAGGGATCG CTTTAATGCA 
AGACAATTCA TGTCTTGGTT ACAAGATGTG GATGATAAAT TTGACAAATT AAAGACCTGT 
CTTTTAATGA GGCAACAACA TGAAGCTGCG GCTTTAAATG CTGTCCAGAG GTTAGAATGG 
CAGCTCAAAC TCCAGGAACT TGATCCTGCC ACCTATAAAT CTATCAGCAT TTACGAAATC 
CAGGAGTTTT ATGTTCCCCT TGTTGATGTT AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( NM_001204056.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001190985.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant 3, mRNA.

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CloneID	OHu10552
Clone ID Related Accession (Same CDS sequence)	XM_017025663.1 , NM_001083625.3 , NM_001083625.2 , XM_017025663.2 , NM_001204056.1
Accession Version	NM_001204056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP005263.3, AY533564.2, AP001033.6 and BM992146.1. Transcript Variant: This variant (3) differs in the 5' UTR and lacks an in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2 and 3 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY533564.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAGTCC AGATTCCACA CCAAATCATC CATCACAAAC AACGCCTGCC 
CAAAAGAAAA CTCCCAGTTC TTCATCTCGA CAGAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCTGCT ATTCGAGGAG ATGTGAAACA AGTTAAAGAA 
TTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ATGTTGGATA TTACGATGTT GCTAAGATAC TTATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCTGCTAG TAGTGGGCAC 
AGAGATATAG TAAAGCTGTT ACTTCGTCAC GGTGGAAATC CATTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTGGATGT AGCAGAAACA GAGGAGTTGG AGTTGCTACT AAAAAGAGAG 
GTGCCTTTAT CTGATGATGA TGAAAGTTAC ACAGATTCCG AAGAGGCTCA ATCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCT GAAACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TACTTCCCAG TAAAACACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGATGAAGA AATTAATAAG ATGATTGATG ATAGGCATAT TCTTAGGAAA 
GAACAACGAA AAGAAAATGA ACCTGAAGCA GAAAAAACTC ATTTATTTGC AAAACAGGAG 
AAAGCCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTCTTATAT 
TCAAGTACTG AAAGTTCTGA TGAAGAAGCT CTTCAGAATA AAAAGATTTC TACTTCATGT 
TCCGTCATCC CTGAAACATC AAATTCTGAT ATGCAAACCA AAAAGGAATA TGTAGTTTCA 
GGTGAACACA AACAGAAAGG CAAAGTTAAA AGAAAATTGA AAAATCAGAA TAAAAATAAA 
GAGAACCAAG AGCTAAAGCA AGAAAAGGAA GGAAAAGAAA ATACAAGAAT AACAAACTTG 
ACAGTAAATA CTGGACTAGA TTGTTCAGAA AAGACCAGAG AGGAGGGGAA CTTTAGGAAA 
TCTTTTAGCC CAAAAGATGA TACTTCATTA CATTTATTTC ATATTTCCAC TGGTAAATCT 
CCCAAACATT CTTGTGGATT AAGTGAAAAA CAGTCAACAC CACTAAAACA AGAACATACT 
AAAACATGTT TATCACCAGG AAGTTCTGAA ATGTCATTAC AGCCTGATCT TGTTCGGTAT 
GATAATACAG AATCTGAATT CTTGCCAGAA AGTTCAAGTG TAAAATCTTG TAAGCATAAG 
GAAAAAAGCA AACATCAGAA AGATTTCCAC TTAGAATTTG GTGAAAAATC AAATGCCAAA 
ATAAAGGATG AAGATCATAG TCCAACATTT GAAAATTCAG ATTGCACACT GAAAAAAATG 
GATAAAGAAG GTAAAACATT AAAAAAACAT AAATTGAAGC ATAAAGAGAG GGAAAAAGAA 
AAGCATAAAA AAGAAATTGA AGGTGAAAAG GAAAAATACA AAACTAAGGA TAGTGCCAAA 
GAACTGCAGA GGAGTGTGGA ATTTGATAGA GAATTTTGGA AAGAGAATTT TTTTAAAAGT 
GATGAAACTG AAGATCTCTT TTTAAATATG GAACATGAAT CCTTAACATT AGAAAAAAAA 
TCAAAATTGG AAAAAAACAT CAAAGATGAT AAATCAACCA AGGAAAAGCA TGTGTCAAAA 
GAGAGGAACT TTAAAGAGGA ACGAGACAAG ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG 
GAGGAAAAAA TAAAAGATCT AAAAGAAGAG AGAGAAAACA TACCCACAGA TAAAGACTCA 
GAATTTACTT CTTTGGGTAT GAGTGCCATT GAGGAATCTA TAGGGCTTCA TTTAGTGGAA 
AAGGAAATAG ACATTGAAAA ACAAGAAAAG CATATAAAGG AAAGTAAAGA AAAACCTGAG 
AAGCGATCTC AAATTAAAGA AAAGGACATT GAGAAGATGG AAAGAAAAAC CTTTGAAAAA 
GAAAAGAAGA TAAAACATGA GCATAAGTCA GAAAAAGACA AATTAGATCT TAGTGAATGT 
GTTGATAAAA TAAAAGAAAA GGACAAGCTA TATTCGCATC ACACAGAAAA ATGCCATAAA 
GAAGGTGAGA AGAGCAAAAA TACTGCTGCT ATTAAAAAAA CTGACGACAG AGAGAAAAGT 
AGAGAAAAGA TGGATAGGAA ACATGACAAA GAAAAGCCTG AAAAAGAGAG GCATCTAGCA 
GAAAGCAAAG AAAAGCACTT GATGGAGAAA AAAAATAAAC AATCAGATAA TAGTGAATAC 
AGTAAATCAG AAAAAGGCAA AAATAAAGAA AAAGACAGGG AGCTAGATAA AAAGGAAAAA 
TCTAGAGATA AAGAAAGTAT AAATATAACT AACTCCAAAC ACATACAGGA AGAAAAAAAA 
TCAAGTATAG TAGACGGTAA TAAAGCACAA CATGAAAAAC CCTTATCCCT TAAAGAAAAA 
ACAAAAGATG AACCTTTGAA AACTCCAGAT GGAAAAGAAA AAGATAAAAA AGATAAAGAT 
ATAGATAGAT ACAAAGAACG AGACAAACAT AAAGATAAAA TTCAAATAAA TAGCTTACTC 
AAACTAAAAT CTGAAGCAGA TAAGCCTAAA CCTAAGTCAT CACCAGCATC AAAAGATACC 
CGACCTAAAG AAAAGAGGTT AGTGAATGAT GATTTAATGC AGACAAGTTT TGAACGAATG 
CTAAGCCTTA AAGACCTAGA AATAGAACAG TGGCACAAAA AACATAAGGA AAAAATTAAG 
CAAAAAGAAA AGGAACGGTT GAGAAACCGA AACTGTTTAG AACTTAAAAT AAAAGATAAA 
GAAAAAACAA AGCATACACC AACTGAATCC AAAAATAAAG AACTTACTAG GTCAAAGAGT 
TCAGAAGTGA CTGATGCATA TACCAAGGAG AAACAACCTA AAGATGCTGT AAGTAACAGA 
TCACAATCTG TTGACACCAA AAATGTAATG ACTTTAGGGA AGTCATCTTT TGTTTCAGAT 
AATAGCTTAA ACAGGTCTCC TAGATCAGAA AATGAAAAGC CGGGTCTCAG CTCCAGATCT 
GTATCCATGA TTTCTGTTGC TAGTTCAGAA GATTCCTGCC ATACTACAGT GACAACCCCA 
AGGCCTCCAG TTGAGTATGA CTCTGACTTT ATGTTAGAGA GTTCAGAATC CCAAATGTCC 
TTTTCCCAGT CACCTTTTTT GTCAATTGCC AAATCTCCTG CTCTTCATGA AAGGGAATTG 
GACAGCCTGG CTGACTTGCC GGAGCGGATT AAACCACCAT ATGCAAACAG ACTTTCAACA 
TCCCATCTTA GGTCATCTTC TGTAGAAGAT GTTAAACTAA TTATAAGCGA GGGGAGACCT 
ACCATAGAAG TTCGAAGATG TAGCATGCCT TCTGTCATTT GTGAACATAC CAAACAATTC 
CAAACAATAT CAGAAGAGAG CAATCAAGGT AGCTTATTAA CTGTGCCAGG AGATACTAGT 
CCTTCTCCCA AACCTGAGGT ATTCTCAAAT GTGCCTGAAA GAGACCTTTC AAATGTATCT 
AACATACATT CCAGTTTTGC AACTTCTCCA ACTGGAGCTT CAAACAGCAA GTATGTTTCA 
GCTGATAGAA ATCTCATCAA GAATACTGCC CCAGTGAACA CTGTAATGGA CAGTCCAGTG 
CATTTAGAGC CATCTAGTCA GGTTGGTGTG ATCCAGAATA AATCATGGGA GATGCCTGTT 
GATAGACTAG AGACATTAAG CACCAGAGAC TTTATCTGCC CAAATTCTAA CATACCTGAT 
CAAGAATCCT CTCTTCAGAG TTTTTGTAAT TCTGAAAATA AGGTATTGAA AGAAAATGCT 
GATTTTTTAT CCCTGCGCCA GACTGAACTG CCAGGAAACT CTTGTGCTCA GGATCCGGCA 
TCCTTTATGC CTCCACAGCA GCCTTGCTCT TTCCCCAGCC AATCACTTTC AGATGCTGAA 
TCGATTTCTA AACATATGTC TTTGTCATAT GTTGCTAATC AAGAGCCAGG TATTTTACAA 
CAAAAAAATG CAGTTCAGAT TATTAGTTCT GCTTTAGATA CTGATAATGA ATCTACAAAA 
GATACAGAAA ATACTTTTGT CCTAGGAGAT GTTCAAAAAA CAGATGCCTT TGTCCCAGTG 
TACTCTGACA GCACTATTCA AGAAGCATCA CCAAACTTTG AGAAAGCTTA TACTTTACCT 
GTGTTACCAT CAGAAAAGGA CTTTAATGGA AGTGATGCCT CTACCCAGCT AAATACACAT 
TATGCATTTA GCAAACTAAC TTACAAGTCT TCCAGTGGCC ATGAAGTTGA GAATAGCACA 
ACTGATACTC AGGTCATTTC ACATGAAAAA GAAAACAAAC TGGAGAGTTT GGTTTTAACT 
CATTTGAGTA GGTGTGATTC TGATTTATGT GAAATGAATG CAGGGATGCC AAAAGGAAAC 
CTAAATGAAC AAGATCCAAA ACATTGTCCT GAAAGTGAAA AGTGTTTGCT TTCCATAGAA 
GATGAGGAAT CTCAACAAAG CATTTTATCA AGTCTGGAAA ACCATTCACA GCAGTCAACT 
CAACCAGAAA TGCATAAATA TGGTCAGTTA GTTAAAGTAG AATTAGAAGA AAATGCCGAA 
GATGATAAAA CTGAAAACCA AATCCCTCAA AGAATGACTA GAAACAAAGC AAATACAATG 
GCAAATCAAA GCAAACAGAT TCTTGCTAGC TGTACACTAT TATCAGAAAA AGACAGTGAA 
TCCTCATCTC CTAGAGGAAG AATAAGATTA ACTGAAGATG ACGATCCTCA AATTCACCAT 
CCACGGAAAA GGAAAGTGTC ACGTGTACCT CAGCCTGTGC AAGTGAGTCC CTCTTTACTA 
CAAGCAAAAG AGAAAACTCA GCAATCTCTG GCAGCCATTG TAGATTCTCT AAAACTAGAT 
GAGATTCAGC CATACAGTTC AGAGAGAGCA AATCCATATT TTGAATACTT GCACATAAGG 
AAAAAAATAG AAGAAAAACG CAAATTACTG TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC 
TATGACGAAT ATGTAACATT TAACGGATCA TATCTCCTGG ATGGAAACCC CTTAAGCAAG 
ATTTGTATTC CCACAATTAC ACCACCACCT TCACTGTCAG ATCCACTTAA AGAGCTTTTT 
CGACAACAGG AAGTTGTAAG GATGAAACTA CGTTTGCAAC ACAGTATTGA AAGGGAAAAA 
CTCATTGTAT CCAACGAACA AGAAGTTTTG CGAGTTCATT ACAGAGCTGC AAGAACATTG 
GCAAATCAAA CACTGCCATT TAGTGCTTGT ACTGTTTTGC TGGATGCCGA AGTATACAAT 
GTACCATTGG ACTCTCAGTC TGATGACAGT AAAACTTCTG TGAGGGATCG CTTTAATGCA 
AGACAATTCA TGTCTTGGTT ACAAGATGTG GATGATAAAT TTGACAAATT AAAGACCTGT 
CTTTTAATGA GGCAACAACA TGAAGCTGCG GCTTTAAATG CTGTCCAGAG GTTAGAATGG 
CAGCTCAAAC TCCAGGAACT TGATCCTGCC ACCTATAAAT CTATCAGCAT TTACGAAATC 
CAGGAGTTTT ATGTTCCCCT TGTTGATGTT AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190985.1
CDS	363..6482
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	NM_001204056.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204056.1

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAGTCC AGATTCCACA CCAAATCATC CATCACAAAC AACGCCTGCC 
CAAAAGAAAA CTCCCAGTTC TTCATCTCGA CAGAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCTGCT ATTCGAGGAG ATGTGAAACA AGTTAAAGAA 
TTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ATGTTGGATA TTACGATGTT GCTAAGATAC TTATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCTGCTAG TAGTGGGCAC 
AGAGATATAG TAAAGCTGTT ACTTCGTCAC GGTGGAAATC CATTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTGGATGT AGCAGAAACA GAGGAGTTGG AGTTGCTACT AAAAAGAGAG 
GTGCCTTTAT CTGATGATGA TGAAAGTTAC ACAGATTCCG AAGAGGCTCA ATCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCT GAAACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TACTTCCCAG TAAAACACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGATGAAGA AATTAATAAG ATGATTGATG ATAGGCATAT TCTTAGGAAA 
GAACAACGAA AAGAAAATGA ACCTGAAGCA GAAAAAACTC ATTTATTTGC AAAACAGGAG 
AAAGCCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTCTTATAT 
TCAAGTACTG AAAGTTCTGA TGAAGAAGCT CTTCAGAATA AAAAGATTTC TACTTCATGT 
TCCGTCATCC CTGAAACATC AAATTCTGAT ATGCAAACCA AAAAGGAATA TGTAGTTTCA 
GGTGAACACA AACAGAAAGG CAAAGTTAAA AGAAAATTGA AAAATCAGAA TAAAAATAAA 
GAGAACCAAG AGCTAAAGCA AGAAAAGGAA GGAAAAGAAA ATACAAGAAT AACAAACTTG 
ACAGTAAATA CTGGACTAGA TTGTTCAGAA AAGACCAGAG AGGAGGGGAA CTTTAGGAAA 
TCTTTTAGCC CAAAAGATGA TACTTCATTA CATTTATTTC ATATTTCCAC TGGTAAATCT 
CCCAAACATT CTTGTGGATT AAGTGAAAAA CAGTCAACAC CACTAAAACA AGAACATACT 
AAAACATGTT TATCACCAGG AAGTTCTGAA ATGTCATTAC AGCCTGATCT TGTTCGGTAT 
GATAATACAG AATCTGAATT CTTGCCAGAA AGTTCAAGTG TAAAATCTTG TAAGCATAAG 
GAAAAAAGCA AACATCAGAA AGATTTCCAC TTAGAATTTG GTGAAAAATC AAATGCCAAA 
ATAAAGGATG AAGATCATAG TCCAACATTT GAAAATTCAG ATTGCACACT GAAAAAAATG 
GATAAAGAAG GTAAAACATT AAAAAAACAT AAATTGAAGC ATAAAGAGAG GGAAAAAGAA 
AAGCATAAAA AAGAAATTGA AGGTGAAAAG GAAAAATACA AAACTAAGGA TAGTGCCAAA 
GAACTGCAGA GGAGTGTGGA ATTTGATAGA GAATTTTGGA AAGAGAATTT TTTTAAAAGT 
GATGAAACTG AAGATCTCTT TTTAAATATG GAACATGAAT CCTTAACATT AGAAAAAAAA 
TCAAAATTGG AAAAAAACAT CAAAGATGAT AAATCAACCA AGGAAAAGCA TGTGTCAAAA 
GAGAGGAACT TTAAAGAGGA ACGAGACAAG ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG 
GAGGAAAAAA TAAAAGATCT AAAAGAAGAG AGAGAAAACA TACCCACAGA TAAAGACTCA 
GAATTTACTT CTTTGGGTAT GAGTGCCATT GAGGAATCTA TAGGGCTTCA TTTAGTGGAA 
AAGGAAATAG ACATTGAAAA ACAAGAAAAG CATATAAAGG AAAGTAAAGA AAAACCTGAG 
AAGCGATCTC AAATTAAAGA AAAGGACATT GAGAAGATGG AAAGAAAAAC CTTTGAAAAA 
GAAAAGAAGA TAAAACATGA GCATAAGTCA GAAAAAGACA AATTAGATCT TAGTGAATGT 
GTTGATAAAA TAAAAGAAAA GGACAAGCTA TATTCGCATC ACACAGAAAA ATGCCATAAA 
GAAGGTGAGA AGAGCAAAAA TACTGCTGCT ATTAAAAAAA CTGACGACAG AGAGAAAAGT 
AGAGAAAAGA TGGATAGGAA ACATGACAAA GAAAAGCCTG AAAAAGAGAG GCATCTAGCA 
GAAAGCAAAG AAAAGCACTT GATGGAGAAA AAAAATAAAC AATCAGATAA TAGTGAATAC 
AGTAAATCAG AAAAAGGCAA AAATAAAGAA AAAGACAGGG AGCTAGATAA AAAGGAAAAA 
TCTAGAGATA AAGAAAGTAT AAATATAACT AACTCCAAAC ACATACAGGA AGAAAAAAAA 
TCAAGTATAG TAGACGGTAA TAAAGCACAA CATGAAAAAC CCTTATCCCT TAAAGAAAAA 
ACAAAAGATG AACCTTTGAA AACTCCAGAT GGAAAAGAAA AAGATAAAAA AGATAAAGAT 
ATAGATAGAT ACAAAGAACG AGACAAACAT AAAGATAAAA TTCAAATAAA TAGCTTACTC 
AAACTAAAAT CTGAAGCAGA TAAGCCTAAA CCTAAGTCAT CACCAGCATC AAAAGATACC 
CGACCTAAAG AAAAGAGGTT AGTGAATGAT GATTTAATGC AGACAAGTTT TGAACGAATG 
CTAAGCCTTA AAGACCTAGA AATAGAACAG TGGCACAAAA AACATAAGGA AAAAATTAAG 
CAAAAAGAAA AGGAACGGTT GAGAAACCGA AACTGTTTAG AACTTAAAAT AAAAGATAAA 
GAAAAAACAA AGCATACACC AACTGAATCC AAAAATAAAG AACTTACTAG GTCAAAGAGT 
TCAGAAGTGA CTGATGCATA TACCAAGGAG AAACAACCTA AAGATGCTGT AAGTAACAGA 
TCACAATCTG TTGACACCAA AAATGTAATG ACTTTAGGGA AGTCATCTTT TGTTTCAGAT 
AATAGCTTAA ACAGGTCTCC TAGATCAGAA AATGAAAAGC CGGGTCTCAG CTCCAGATCT 
GTATCCATGA TTTCTGTTGC TAGTTCAGAA GATTCCTGCC ATACTACAGT GACAACCCCA 
AGGCCTCCAG TTGAGTATGA CTCTGACTTT ATGTTAGAGA GTTCAGAATC CCAAATGTCC 
TTTTCCCAGT CACCTTTTTT GTCAATTGCC AAATCTCCTG CTCTTCATGA AAGGGAATTG 
GACAGCCTGG CTGACTTGCC GGAGCGGATT AAACCACCAT ATGCAAACAG ACTTTCAACA 
TCCCATCTTA GGTCATCTTC TGTAGAAGAT GTTAAACTAA TTATAAGCGA GGGGAGACCT 
ACCATAGAAG TTCGAAGATG TAGCATGCCT TCTGTCATTT GTGAACATAC CAAACAATTC 
CAAACAATAT CAGAAGAGAG CAATCAAGGT AGCTTATTAA CTGTGCCAGG AGATACTAGT 
CCTTCTCCCA AACCTGAGGT ATTCTCAAAT GTGCCTGAAA GAGACCTTTC AAATGTATCT 
AACATACATT CCAGTTTTGC AACTTCTCCA ACTGGAGCTT CAAACAGCAA GTATGTTTCA 
GCTGATAGAA ATCTCATCAA GAATACTGCC CCAGTGAACA CTGTAATGGA CAGTCCAGTG 
CATTTAGAGC CATCTAGTCA GGTTGGTGTG ATCCAGAATA AATCATGGGA GATGCCTGTT 
GATAGACTAG AGACATTAAG CACCAGAGAC TTTATCTGCC CAAATTCTAA CATACCTGAT 
CAAGAATCCT CTCTTCAGAG TTTTTGTAAT TCTGAAAATA AGGTATTGAA AGAAAATGCT 
GATTTTTTAT CCCTGCGCCA GACTGAACTG CCAGGAAACT CTTGTGCTCA GGATCCGGCA 
TCCTTTATGC CTCCACAGCA GCCTTGCTCT TTCCCCAGCC AATCACTTTC AGATGCTGAA 
TCGATTTCTA AACATATGTC TTTGTCATAT GTTGCTAATC AAGAGCCAGG TATTTTACAA 
CAAAAAAATG CAGTTCAGAT TATTAGTTCT GCTTTAGATA CTGATAATGA ATCTACAAAA 
GATACAGAAA ATACTTTTGT CCTAGGAGAT GTTCAAAAAA CAGATGCCTT TGTCCCAGTG 
TACTCTGACA GCACTATTCA AGAAGCATCA CCAAACTTTG AGAAAGCTTA TACTTTACCT 
GTGTTACCAT CAGAAAAGGA CTTTAATGGA AGTGATGCCT CTACCCAGCT AAATACACAT 
TATGCATTTA GCAAACTAAC TTACAAGTCT TCCAGTGGCC ATGAAGTTGA GAATAGCACA 
ACTGATACTC AGGTCATTTC ACATGAAAAA GAAAACAAAC TGGAGAGTTT GGTTTTAACT 
CATTTGAGTA GGTGTGATTC TGATTTATGT GAAATGAATG CAGGGATGCC AAAAGGAAAC 
CTAAATGAAC AAGATCCAAA ACATTGTCCT GAAAGTGAAA AGTGTTTGCT TTCCATAGAA 
GATGAGGAAT CTCAACAAAG CATTTTATCA AGTCTGGAAA ACCATTCACA GCAGTCAACT 
CAACCAGAAA TGCATAAATA TGGTCAGTTA GTTAAAGTAG AATTAGAAGA AAATGCCGAA 
GATGATAAAA CTGAAAACCA AATCCCTCAA AGAATGACTA GAAACAAAGC AAATACAATG 
GCAAATCAAA GCAAACAGAT TCTTGCTAGC TGTACACTAT TATCAGAAAA AGACAGTGAA 
TCCTCATCTC CTAGAGGAAG AATAAGATTA ACTGAAGATG ACGATCCTCA AATTCACCAT 
CCACGGAAAA GGAAAGTGTC ACGTGTACCT CAGCCTGTGC AAGTGAGTCC CTCTTTACTA 
CAAGCAAAAG AGAAAACTCA GCAATCTCTG GCAGCCATTG TAGATTCTCT AAAACTAGAT 
GAGATTCAGC CATACAGTTC AGAGAGAGCA AATCCATATT TTGAATACTT GCACATAAGG 
AAAAAAATAG AAGAAAAACG CAAATTACTG TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC 
TATGACGAAT ATGTAACATT TAACGGATCA TATCTCCTGG ATGGAAACCC CTTAAGCAAG 
ATTTGTATTC CCACAATTAC ACCACCACCT TCACTGTCAG ATCCACTTAA AGAGCTTTTT 
CGACAACAGG AAGTTGTAAG GATGAAACTA CGTTTGCAAC ACAGTATTGA AAGGGAAAAA 
CTCATTGTAT CCAACGAACA AGAAGTTTTG CGAGTTCATT ACAGAGCTGC AAGAACATTG 
GCAAATCAAA CACTGCCATT TAGTGCTTGT ACTGTTTTGC TGGATGCCGA AGTATACAAT 
GTACCATTGG ACTCTCAGTC TGATGACAGT AAAACTTCTG TGAGGGATCG CTTTAATGCA 
AGACAATTCA TGTCTTGGTT ACAAGATGTG GATGATAAAT TTGACAAATT AAAGACCTGT 
CTTTTAATGA GGCAACAACA TGAAGCTGCG GCTTTAAATG CTGTCCAGAG GTTAGAATGG 
CAGCTCAAAC TCCAGGAACT TGATCCTGCC ACCTATAAAT CTATCAGCAT TTACGAAATC 
CAGGAGTTTT ATGTTCCCCT TGTTGATGTT AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025663.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881152.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X6, mRNA.

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CloneID	OHu10552
Clone ID Related Accession (Same CDS sequence)	XM_017025663.1 , NM_001083625.3 , NM_001083625.2 , XM_017025663.2 , NM_001204056.1
Accession Version	XM_017025663.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAGTCC AGATTCCACA CCAAATCATC CATCACAAAC AACGCCTGCC 
CAAAAGAAAA CTCCCAGTTC TTCATCTCGA CAGAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCTGCT ATTCGAGGAG ATGTGAAACA AGTTAAAGAA 
TTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ATGTTGGATA TTACGATGTT GCTAAGATAC TTATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCTGCTAG TAGTGGGCAC 
AGAGATATAG TAAAGCTGTT ACTTCGTCAC GGTGGAAATC CATTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTGGATGT AGCAGAAACA GAGGAGTTGG AGTTGCTACT AAAAAGAGAG 
GTGCCTTTAT CTGATGATGA TGAAAGTTAC ACAGATTCCG AAGAGGCTCA ATCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCT GAAACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TACTTCCCAG TAAAACACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGATGAAGA AATTAATAAG ATGATTGATG ATAGGCATAT TCTTAGGAAA 
GAACAACGAA AAGAAAATGA ACCTGAAGCA GAAAAAACTC ATTTATTTGC AAAACAGGAG 
AAAGCCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTCTTATAT 
TCAAGTACTG AAAGTTCTGA TGAAGAAGCT CTTCAGAATA AAAAGATTTC TACTTCATGT 
TCCGTCATCC CTGAAACATC AAATTCTGAT ATGCAAACCA AAAAGGAATA TGTAGTTTCA 
GGTGAACACA AACAGAAAGG CAAAGTTAAA AGAAAATTGA AAAATCAGAA TAAAAATAAA 
GAGAACCAAG AGCTAAAGCA AGAAAAGGAA GGAAAAGAAA ATACAAGAAT AACAAACTTG 
ACAGTAAATA CTGGACTAGA TTGTTCAGAA AAGACCAGAG AGGAGGGGAA CTTTAGGAAA 
TCTTTTAGCC CAAAAGATGA TACTTCATTA CATTTATTTC ATATTTCCAC TGGTAAATCT 
CCCAAACATT CTTGTGGATT AAGTGAAAAA CAGTCAACAC CACTAAAACA AGAACATACT 
AAAACATGTT TATCACCAGG AAGTTCTGAA ATGTCATTAC AGCCTGATCT TGTTCGGTAT 
GATAATACAG AATCTGAATT CTTGCCAGAA AGTTCAAGTG TAAAATCTTG TAAGCATAAG 
GAAAAAAGCA AACATCAGAA AGATTTCCAC TTAGAATTTG GTGAAAAATC AAATGCCAAA 
ATAAAGGATG AAGATCATAG TCCAACATTT GAAAATTCAG ATTGCACACT GAAAAAAATG 
GATAAAGAAG GTAAAACATT AAAAAAACAT AAATTGAAGC ATAAAGAGAG GGAAAAAGAA 
AAGCATAAAA AAGAAATTGA AGGTGAAAAG GAAAAATACA AAACTAAGGA TAGTGCCAAA 
GAACTGCAGA GGAGTGTGGA ATTTGATAGA GAATTTTGGA AAGAGAATTT TTTTAAAAGT 
GATGAAACTG AAGATCTCTT TTTAAATATG GAACATGAAT CCTTAACATT AGAAAAAAAA 
TCAAAATTGG AAAAAAACAT CAAAGATGAT AAATCAACCA AGGAAAAGCA TGTGTCAAAA 
GAGAGGAACT TTAAAGAGGA ACGAGACAAG ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG 
GAGGAAAAAA TAAAAGATCT AAAAGAAGAG AGAGAAAACA TACCCACAGA TAAAGACTCA 
GAATTTACTT CTTTGGGTAT GAGTGCCATT GAGGAATCTA TAGGGCTTCA TTTAGTGGAA 
AAGGAAATAG ACATTGAAAA ACAAGAAAAG CATATAAAGG AAAGTAAAGA AAAACCTGAG 
AAGCGATCTC AAATTAAAGA AAAGGACATT GAGAAGATGG AAAGAAAAAC CTTTGAAAAA 
GAAAAGAAGA TAAAACATGA GCATAAGTCA GAAAAAGACA AATTAGATCT TAGTGAATGT 
GTTGATAAAA TAAAAGAAAA GGACAAGCTA TATTCGCATC ACACAGAAAA ATGCCATAAA 
GAAGGTGAGA AGAGCAAAAA TACTGCTGCT ATTAAAAAAA CTGACGACAG AGAGAAAAGT 
AGAGAAAAGA TGGATAGGAA ACATGACAAA GAAAAGCCTG AAAAAGAGAG GCATCTAGCA 
GAAAGCAAAG AAAAGCACTT GATGGAGAAA AAAAATAAAC AATCAGATAA TAGTGAATAC 
AGTAAATCAG AAAAAGGCAA AAATAAAGAA AAAGACAGGG AGCTAGATAA AAAGGAAAAA 
TCTAGAGATA AAGAAAGTAT AAATATAACT AACTCCAAAC ACATACAGGA AGAAAAAAAA 
TCAAGTATAG TAGACGGTAA TAAAGCACAA CATGAAAAAC CCTTATCCCT TAAAGAAAAA 
ACAAAAGATG AACCTTTGAA AACTCCAGAT GGAAAAGAAA AAGATAAAAA AGATAAAGAT 
ATAGATAGAT ACAAAGAACG AGACAAACAT AAAGATAAAA TTCAAATAAA TAGCTTACTC 
AAACTAAAAT CTGAAGCAGA TAAGCCTAAA CCTAAGTCAT CACCAGCATC AAAAGATACC 
CGACCTAAAG AAAAGAGGTT AGTGAATGAT GATTTAATGC AGACAAGTTT TGAACGAATG 
CTAAGCCTTA AAGACCTAGA AATAGAACAG TGGCACAAAA AACATAAGGA AAAAATTAAG 
CAAAAAGAAA AGGAACGGTT GAGAAACCGA AACTGTTTAG AACTTAAAAT AAAAGATAAA 
GAAAAAACAA AGCATACACC AACTGAATCC AAAAATAAAG AACTTACTAG GTCAAAGAGT 
TCAGAAGTGA CTGATGCATA TACCAAGGAG AAACAACCTA AAGATGCTGT AAGTAACAGA 
TCACAATCTG TTGACACCAA AAATGTAATG ACTTTAGGGA AGTCATCTTT TGTTTCAGAT 
AATAGCTTAA ACAGGTCTCC TAGATCAGAA AATGAAAAGC CGGGTCTCAG CTCCAGATCT 
GTATCCATGA TTTCTGTTGC TAGTTCAGAA GATTCCTGCC ATACTACAGT GACAACCCCA 
AGGCCTCCAG TTGAGTATGA CTCTGACTTT ATGTTAGAGA GTTCAGAATC CCAAATGTCC 
TTTTCCCAGT CACCTTTTTT GTCAATTGCC AAATCTCCTG CTCTTCATGA AAGGGAATTG 
GACAGCCTGG CTGACTTGCC GGAGCGGATT AAACCACCAT ATGCAAACAG ACTTTCAACA 
TCCCATCTTA GGTCATCTTC TGTAGAAGAT GTTAAACTAA TTATAAGCGA GGGGAGACCT 
ACCATAGAAG TTCGAAGATG TAGCATGCCT TCTGTCATTT GTGAACATAC CAAACAATTC 
CAAACAATAT CAGAAGAGAG CAATCAAGGT AGCTTATTAA CTGTGCCAGG AGATACTAGT 
CCTTCTCCCA AACCTGAGGT ATTCTCAAAT GTGCCTGAAA GAGACCTTTC AAATGTATCT 
AACATACATT CCAGTTTTGC AACTTCTCCA ACTGGAGCTT CAAACAGCAA GTATGTTTCA 
GCTGATAGAA ATCTCATCAA GAATACTGCC CCAGTGAACA CTGTAATGGA CAGTCCAGTG 
CATTTAGAGC CATCTAGTCA GGTTGGTGTG ATCCAGAATA AATCATGGGA GATGCCTGTT 
GATAGACTAG AGACATTAAG CACCAGAGAC TTTATCTGCC CAAATTCTAA CATACCTGAT 
CAAGAATCCT CTCTTCAGAG TTTTTGTAAT TCTGAAAATA AGGTATTGAA AGAAAATGCT 
GATTTTTTAT CCCTGCGCCA GACTGAACTG CCAGGAAACT CTTGTGCTCA GGATCCGGCA 
TCCTTTATGC CTCCACAGCA GCCTTGCTCT TTCCCCAGCC AATCACTTTC AGATGCTGAA 
TCGATTTCTA AACATATGTC TTTGTCATAT GTTGCTAATC AAGAGCCAGG TATTTTACAA 
CAAAAAAATG CAGTTCAGAT TATTAGTTCT GCTTTAGATA CTGATAATGA ATCTACAAAA 
GATACAGAAA ATACTTTTGT CCTAGGAGAT GTTCAAAAAA CAGATGCCTT TGTCCCAGTG 
TACTCTGACA GCACTATTCA AGAAGCATCA CCAAACTTTG AGAAAGCTTA TACTTTACCT 
GTGTTACCAT CAGAAAAGGA CTTTAATGGA AGTGATGCCT CTACCCAGCT AAATACACAT 
TATGCATTTA GCAAACTAAC TTACAAGTCT TCCAGTGGCC ATGAAGTTGA GAATAGCACA 
ACTGATACTC AGGTCATTTC ACATGAAAAA GAAAACAAAC TGGAGAGTTT GGTTTTAACT 
CATTTGAGTA GGTGTGATTC TGATTTATGT GAAATGAATG CAGGGATGCC AAAAGGAAAC 
CTAAATGAAC AAGATCCAAA ACATTGTCCT GAAAGTGAAA AGTGTTTGCT TTCCATAGAA 
GATGAGGAAT CTCAACAAAG CATTTTATCA AGTCTGGAAA ACCATTCACA GCAGTCAACT 
CAACCAGAAA TGCATAAATA TGGTCAGTTA GTTAAAGTAG AATTAGAAGA AAATGCCGAA 
GATGATAAAA CTGAAAACCA AATCCCTCAA AGAATGACTA GAAACAAAGC AAATACAATG 
GCAAATCAAA GCAAACAGAT TCTTGCTAGC TGTACACTAT TATCAGAAAA AGACAGTGAA 
TCCTCATCTC CTAGAGGAAG AATAAGATTA ACTGAAGATG ACGATCCTCA AATTCACCAT 
CCACGGAAAA GGAAAGTGTC ACGTGTACCT CAGCCTGTGC AAGTGAGTCC CTCTTTACTA 
CAAGCAAAAG AGAAAACTCA GCAATCTCTG GCAGCCATTG TAGATTCTCT AAAACTAGAT 
GAGATTCAGC CATACAGTTC AGAGAGAGCA AATCCATATT TTGAATACTT GCACATAAGG 
AAAAAAATAG AAGAAAAACG CAAATTACTG TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC 
TATGACGAAT ATGTAACATT TAACGGATCA TATCTCCTGG ATGGAAACCC CTTAAGCAAG 
ATTTGTATTC CCACAATTAC ACCACCACCT TCACTGTCAG ATCCACTTAA AGAGCTTTTT 
CGACAACAGG AAGTTGTAAG GATGAAACTA CGTTTGCAAC ACAGTATTGA AAGGGAAAAA 
CTCATTGTAT CCAACGAACA AGAAGTTTTG CGAGTTCATT ACAGAGCTGC AAGAACATTG 
GCAAATCAAA CACTGCCATT TAGTGCTTGT ACTGTTTTGC TGGATGCCGA AGTATACAAT 
GTACCATTGG ACTCTCAGTC TGATGACAGT AAAACTTCTG TGAGGGATCG CTTTAATGCA 
AGACAATTCA TGTCTTGGTT ACAAGATGTG GATGATAAAT TTGACAAATT AAAGACCTGT 
CTTTTAATGA GGCAACAACA TGAAGCTGCG GCTTTAAATG CTGTCCAGAG GTTAGAATGG 
CAGCTCAAAC TCCAGGAACT TGATCCTGCC ACCTATAAAT CTATCAGCAT TTACGAAATC 
CAGGAGTTTT ATGTTCCCCT TGTTGATGTT AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881152.1
CDS	350..6469
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025663.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025663.1

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAGTCC AGATTCCACA CCAAATCATC CATCACAAAC AACGCCTGCC 
CAAAAGAAAA CTCCCAGTTC TTCATCTCGA CAGAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCTGCT ATTCGAGGAG ATGTGAAACA AGTTAAAGAA 
TTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ATGTTGGATA TTACGATGTT GCTAAGATAC TTATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCTGCTAG TAGTGGGCAC 
AGAGATATAG TAAAGCTGTT ACTTCGTCAC GGTGGAAATC CATTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTGGATGT AGCAGAAACA GAGGAGTTGG AGTTGCTACT AAAAAGAGAG 
GTGCCTTTAT CTGATGATGA TGAAAGTTAC ACAGATTCCG AAGAGGCTCA ATCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCT GAAACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TACTTCCCAG TAAAACACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGATGAAGA AATTAATAAG ATGATTGATG ATAGGCATAT TCTTAGGAAA 
GAACAACGAA AAGAAAATGA ACCTGAAGCA GAAAAAACTC ATTTATTTGC AAAACAGGAG 
AAAGCCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTCTTATAT 
TCAAGTACTG AAAGTTCTGA TGAAGAAGCT CTTCAGAATA AAAAGATTTC TACTTCATGT 
TCCGTCATCC CTGAAACATC AAATTCTGAT ATGCAAACCA AAAAGGAATA TGTAGTTTCA 
GGTGAACACA AACAGAAAGG CAAAGTTAAA AGAAAATTGA AAAATCAGAA TAAAAATAAA 
GAGAACCAAG AGCTAAAGCA AGAAAAGGAA GGAAAAGAAA ATACAAGAAT AACAAACTTG 
ACAGTAAATA CTGGACTAGA TTGTTCAGAA AAGACCAGAG AGGAGGGGAA CTTTAGGAAA 
TCTTTTAGCC CAAAAGATGA TACTTCATTA CATTTATTTC ATATTTCCAC TGGTAAATCT 
CCCAAACATT CTTGTGGATT AAGTGAAAAA CAGTCAACAC CACTAAAACA AGAACATACT 
AAAACATGTT TATCACCAGG AAGTTCTGAA ATGTCATTAC AGCCTGATCT TGTTCGGTAT 
GATAATACAG AATCTGAATT CTTGCCAGAA AGTTCAAGTG TAAAATCTTG TAAGCATAAG 
GAAAAAAGCA AACATCAGAA AGATTTCCAC TTAGAATTTG GTGAAAAATC AAATGCCAAA 
ATAAAGGATG AAGATCATAG TCCAACATTT GAAAATTCAG ATTGCACACT GAAAAAAATG 
GATAAAGAAG GTAAAACATT AAAAAAACAT AAATTGAAGC ATAAAGAGAG GGAAAAAGAA 
AAGCATAAAA AAGAAATTGA AGGTGAAAAG GAAAAATACA AAACTAAGGA TAGTGCCAAA 
GAACTGCAGA GGAGTGTGGA ATTTGATAGA GAATTTTGGA AAGAGAATTT TTTTAAAAGT 
GATGAAACTG AAGATCTCTT TTTAAATATG GAACATGAAT CCTTAACATT AGAAAAAAAA 
TCAAAATTGG AAAAAAACAT CAAAGATGAT AAATCAACCA AGGAAAAGCA TGTGTCAAAA 
GAGAGGAACT TTAAAGAGGA ACGAGACAAG ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG 
GAGGAAAAAA TAAAAGATCT AAAAGAAGAG AGAGAAAACA TACCCACAGA TAAAGACTCA 
GAATTTACTT CTTTGGGTAT GAGTGCCATT GAGGAATCTA TAGGGCTTCA TTTAGTGGAA 
AAGGAAATAG ACATTGAAAA ACAAGAAAAG CATATAAAGG AAAGTAAAGA AAAACCTGAG 
AAGCGATCTC AAATTAAAGA AAAGGACATT GAGAAGATGG AAAGAAAAAC CTTTGAAAAA 
GAAAAGAAGA TAAAACATGA GCATAAGTCA GAAAAAGACA AATTAGATCT TAGTGAATGT 
GTTGATAAAA TAAAAGAAAA GGACAAGCTA TATTCGCATC ACACAGAAAA ATGCCATAAA 
GAAGGTGAGA AGAGCAAAAA TACTGCTGCT ATTAAAAAAA CTGACGACAG AGAGAAAAGT 
AGAGAAAAGA TGGATAGGAA ACATGACAAA GAAAAGCCTG AAAAAGAGAG GCATCTAGCA 
GAAAGCAAAG AAAAGCACTT GATGGAGAAA AAAAATAAAC AATCAGATAA TAGTGAATAC 
AGTAAATCAG AAAAAGGCAA AAATAAAGAA AAAGACAGGG AGCTAGATAA AAAGGAAAAA 
TCTAGAGATA AAGAAAGTAT AAATATAACT AACTCCAAAC ACATACAGGA AGAAAAAAAA 
TCAAGTATAG TAGACGGTAA TAAAGCACAA CATGAAAAAC CCTTATCCCT TAAAGAAAAA 
ACAAAAGATG AACCTTTGAA AACTCCAGAT GGAAAAGAAA AAGATAAAAA AGATAAAGAT 
ATAGATAGAT ACAAAGAACG AGACAAACAT AAAGATAAAA TTCAAATAAA TAGCTTACTC 
AAACTAAAAT CTGAAGCAGA TAAGCCTAAA CCTAAGTCAT CACCAGCATC AAAAGATACC 
CGACCTAAAG AAAAGAGGTT AGTGAATGAT GATTTAATGC AGACAAGTTT TGAACGAATG 
CTAAGCCTTA AAGACCTAGA AATAGAACAG TGGCACAAAA AACATAAGGA AAAAATTAAG 
CAAAAAGAAA AGGAACGGTT GAGAAACCGA AACTGTTTAG AACTTAAAAT AAAAGATAAA 
GAAAAAACAA AGCATACACC AACTGAATCC AAAAATAAAG AACTTACTAG GTCAAAGAGT 
TCAGAAGTGA CTGATGCATA TACCAAGGAG AAACAACCTA AAGATGCTGT AAGTAACAGA 
TCACAATCTG TTGACACCAA AAATGTAATG ACTTTAGGGA AGTCATCTTT TGTTTCAGAT 
AATAGCTTAA ACAGGTCTCC TAGATCAGAA AATGAAAAGC CGGGTCTCAG CTCCAGATCT 
GTATCCATGA TTTCTGTTGC TAGTTCAGAA GATTCCTGCC ATACTACAGT GACAACCCCA 
AGGCCTCCAG TTGAGTATGA CTCTGACTTT ATGTTAGAGA GTTCAGAATC CCAAATGTCC 
TTTTCCCAGT CACCTTTTTT GTCAATTGCC AAATCTCCTG CTCTTCATGA AAGGGAATTG 
GACAGCCTGG CTGACTTGCC GGAGCGGATT AAACCACCAT ATGCAAACAG ACTTTCAACA 
TCCCATCTTA GGTCATCTTC TGTAGAAGAT GTTAAACTAA TTATAAGCGA GGGGAGACCT 
ACCATAGAAG TTCGAAGATG TAGCATGCCT TCTGTCATTT GTGAACATAC CAAACAATTC 
CAAACAATAT CAGAAGAGAG CAATCAAGGT AGCTTATTAA CTGTGCCAGG AGATACTAGT 
CCTTCTCCCA AACCTGAGGT ATTCTCAAAT GTGCCTGAAA GAGACCTTTC AAATGTATCT 
AACATACATT CCAGTTTTGC AACTTCTCCA ACTGGAGCTT CAAACAGCAA GTATGTTTCA 
GCTGATAGAA ATCTCATCAA GAATACTGCC CCAGTGAACA CTGTAATGGA CAGTCCAGTG 
CATTTAGAGC CATCTAGTCA GGTTGGTGTG ATCCAGAATA AATCATGGGA GATGCCTGTT 
GATAGACTAG AGACATTAAG CACCAGAGAC TTTATCTGCC CAAATTCTAA CATACCTGAT 
CAAGAATCCT CTCTTCAGAG TTTTTGTAAT TCTGAAAATA AGGTATTGAA AGAAAATGCT 
GATTTTTTAT CCCTGCGCCA GACTGAACTG CCAGGAAACT CTTGTGCTCA GGATCCGGCA 
TCCTTTATGC CTCCACAGCA GCCTTGCTCT TTCCCCAGCC AATCACTTTC AGATGCTGAA 
TCGATTTCTA AACATATGTC TTTGTCATAT GTTGCTAATC AAGAGCCAGG TATTTTACAA 
CAAAAAAATG CAGTTCAGAT TATTAGTTCT GCTTTAGATA CTGATAATGA ATCTACAAAA 
GATACAGAAA ATACTTTTGT CCTAGGAGAT GTTCAAAAAA CAGATGCCTT TGTCCCAGTG 
TACTCTGACA GCACTATTCA AGAAGCATCA CCAAACTTTG AGAAAGCTTA TACTTTACCT 
GTGTTACCAT CAGAAAAGGA CTTTAATGGA AGTGATGCCT CTACCCAGCT AAATACACAT 
TATGCATTTA GCAAACTAAC TTACAAGTCT TCCAGTGGCC ATGAAGTTGA GAATAGCACA 
ACTGATACTC AGGTCATTTC ACATGAAAAA GAAAACAAAC TGGAGAGTTT GGTTTTAACT 
CATTTGAGTA GGTGTGATTC TGATTTATGT GAAATGAATG CAGGGATGCC AAAAGGAAAC 
CTAAATGAAC AAGATCCAAA ACATTGTCCT GAAAGTGAAA AGTGTTTGCT TTCCATAGAA 
GATGAGGAAT CTCAACAAAG CATTTTATCA AGTCTGGAAA ACCATTCACA GCAGTCAACT 
CAACCAGAAA TGCATAAATA TGGTCAGTTA GTTAAAGTAG AATTAGAAGA AAATGCCGAA 
GATGATAAAA CTGAAAACCA AATCCCTCAA AGAATGACTA GAAACAAAGC AAATACAATG 
GCAAATCAAA GCAAACAGAT TCTTGCTAGC TGTACACTAT TATCAGAAAA AGACAGTGAA 
TCCTCATCTC CTAGAGGAAG AATAAGATTA ACTGAAGATG ACGATCCTCA AATTCACCAT 
CCACGGAAAA GGAAAGTGTC ACGTGTACCT CAGCCTGTGC AAGTGAGTCC CTCTTTACTA 
CAAGCAAAAG AGAAAACTCA GCAATCTCTG GCAGCCATTG TAGATTCTCT AAAACTAGAT 
GAGATTCAGC CATACAGTTC AGAGAGAGCA AATCCATATT TTGAATACTT GCACATAAGG 
AAAAAAATAG AAGAAAAACG CAAATTACTG TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC 
TATGACGAAT ATGTAACATT TAACGGATCA TATCTCCTGG ATGGAAACCC CTTAAGCAAG 
ATTTGTATTC CCACAATTAC ACCACCACCT TCACTGTCAG ATCCACTTAA AGAGCTTTTT 
CGACAACAGG AAGTTGTAAG GATGAAACTA CGTTTGCAAC ACAGTATTGA AAGGGAAAAA 
CTCATTGTAT CCAACGAACA AGAAGTTTTG CGAGTTCATT ACAGAGCTGC AAGAACATTG 
GCAAATCAAA CACTGCCATT TAGTGCTTGT ACTGTTTTGC TGGATGCCGA AGTATACAAT 
GTACCATTGG ACTCTCAGTC TGATGACAGT AAAACTTCTG TGAGGGATCG CTTTAATGCA 
AGACAATTCA TGTCTTGGTT ACAAGATGTG GATGATAAAT TTGACAAATT AAAGACCTGT 
CTTTTAATGA GGCAACAACA TGAAGCTGCG GCTTTAAATG CTGTCCAGAG GTTAGAATGG 
CAGCTCAAAC TCCAGGAACT TGATCCTGCC ACCTATAAAT CTATCAGCAT TTACGAAATC 
CAGGAGTTTT ATGTTCCCCT TGTTGATGTT AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025663.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881152.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X7, mRNA.

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CloneID	OHu10552
Clone ID Related Accession (Same CDS sequence)	XM_017025663.1 , NM_001083625.3 , NM_001083625.2 , XM_017025663.2 , NM_001204056.1
Accession Version	XM_017025663.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025663.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAGTCC AGATTCCACA CCAAATCATC CATCACAAAC AACGCCTGCC 
CAAAAGAAAA CTCCCAGTTC TTCATCTCGA CAGAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCTGCT ATTCGAGGAG ATGTGAAACA AGTTAAAGAA 
TTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ATGTTGGATA TTACGATGTT GCTAAGATAC TTATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCTGCTAG TAGTGGGCAC 
AGAGATATAG TAAAGCTGTT ACTTCGTCAC GGTGGAAATC CATTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTGGATGT AGCAGAAACA GAGGAGTTGG AGTTGCTACT AAAAAGAGAG 
GTGCCTTTAT CTGATGATGA TGAAAGTTAC ACAGATTCCG AAGAGGCTCA ATCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCT GAAACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TACTTCCCAG TAAAACACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGATGAAGA AATTAATAAG ATGATTGATG ATAGGCATAT TCTTAGGAAA 
GAACAACGAA AAGAAAATGA ACCTGAAGCA GAAAAAACTC ATTTATTTGC AAAACAGGAG 
AAAGCCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTCTTATAT 
TCAAGTACTG AAAGTTCTGA TGAAGAAGCT CTTCAGAATA AAAAGATTTC TACTTCATGT 
TCCGTCATCC CTGAAACATC AAATTCTGAT ATGCAAACCA AAAAGGAATA TGTAGTTTCA 
GGTGAACACA AACAGAAAGG CAAAGTTAAA AGAAAATTGA AAAATCAGAA TAAAAATAAA 
GAGAACCAAG AGCTAAAGCA AGAAAAGGAA GGAAAAGAAA ATACAAGAAT AACAAACTTG 
ACAGTAAATA CTGGACTAGA TTGTTCAGAA AAGACCAGAG AGGAGGGGAA CTTTAGGAAA 
TCTTTTAGCC CAAAAGATGA TACTTCATTA CATTTATTTC ATATTTCCAC TGGTAAATCT 
CCCAAACATT CTTGTGGATT AAGTGAAAAA CAGTCAACAC CACTAAAACA AGAACATACT 
AAAACATGTT TATCACCAGG AAGTTCTGAA ATGTCATTAC AGCCTGATCT TGTTCGGTAT 
GATAATACAG AATCTGAATT CTTGCCAGAA AGTTCAAGTG TAAAATCTTG TAAGCATAAG 
GAAAAAAGCA AACATCAGAA AGATTTCCAC TTAGAATTTG GTGAAAAATC AAATGCCAAA 
ATAAAGGATG AAGATCATAG TCCAACATTT GAAAATTCAG ATTGCACACT GAAAAAAATG 
GATAAAGAAG GTAAAACATT AAAAAAACAT AAATTGAAGC ATAAAGAGAG GGAAAAAGAA 
AAGCATAAAA AAGAAATTGA AGGTGAAAAG GAAAAATACA AAACTAAGGA TAGTGCCAAA 
GAACTGCAGA GGAGTGTGGA ATTTGATAGA GAATTTTGGA AAGAGAATTT TTTTAAAAGT 
GATGAAACTG AAGATCTCTT TTTAAATATG GAACATGAAT CCTTAACATT AGAAAAAAAA 
TCAAAATTGG AAAAAAACAT CAAAGATGAT AAATCAACCA AGGAAAAGCA TGTGTCAAAA 
GAGAGGAACT TTAAAGAGGA ACGAGACAAG ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG 
GAGGAAAAAA TAAAAGATCT AAAAGAAGAG AGAGAAAACA TACCCACAGA TAAAGACTCA 
GAATTTACTT CTTTGGGTAT GAGTGCCATT GAGGAATCTA TAGGGCTTCA TTTAGTGGAA 
AAGGAAATAG ACATTGAAAA ACAAGAAAAG CATATAAAGG AAAGTAAAGA AAAACCTGAG 
AAGCGATCTC AAATTAAAGA AAAGGACATT GAGAAGATGG AAAGAAAAAC CTTTGAAAAA 
GAAAAGAAGA TAAAACATGA GCATAAGTCA GAAAAAGACA AATTAGATCT TAGTGAATGT 
GTTGATAAAA TAAAAGAAAA GGACAAGCTA TATTCGCATC ACACAGAAAA ATGCCATAAA 
GAAGGTGAGA AGAGCAAAAA TACTGCTGCT ATTAAAAAAA CTGACGACAG AGAGAAAAGT 
AGAGAAAAGA TGGATAGGAA ACATGACAAA GAAAAGCCTG AAAAAGAGAG GCATCTAGCA 
GAAAGCAAAG AAAAGCACTT GATGGAGAAA AAAAATAAAC AATCAGATAA TAGTGAATAC 
AGTAAATCAG AAAAAGGCAA AAATAAAGAA AAAGACAGGG AGCTAGATAA AAAGGAAAAA 
TCTAGAGATA AAGAAAGTAT AAATATAACT AACTCCAAAC ACATACAGGA AGAAAAAAAA 
TCAAGTATAG TAGACGGTAA TAAAGCACAA CATGAAAAAC CCTTATCCCT TAAAGAAAAA 
ACAAAAGATG AACCTTTGAA AACTCCAGAT GGAAAAGAAA AAGATAAAAA AGATAAAGAT 
ATAGATAGAT ACAAAGAACG AGACAAACAT AAAGATAAAA TTCAAATAAA TAGCTTACTC 
AAACTAAAAT CTGAAGCAGA TAAGCCTAAA CCTAAGTCAT CACCAGCATC AAAAGATACC 
CGACCTAAAG AAAAGAGGTT AGTGAATGAT GATTTAATGC AGACAAGTTT TGAACGAATG 
CTAAGCCTTA AAGACCTAGA AATAGAACAG TGGCACAAAA AACATAAGGA AAAAATTAAG 
CAAAAAGAAA AGGAACGGTT GAGAAACCGA AACTGTTTAG AACTTAAAAT AAAAGATAAA 
GAAAAAACAA AGCATACACC AACTGAATCC AAAAATAAAG AACTTACTAG GTCAAAGAGT 
TCAGAAGTGA CTGATGCATA TACCAAGGAG AAACAACCTA AAGATGCTGT AAGTAACAGA 
TCACAATCTG TTGACACCAA AAATGTAATG ACTTTAGGGA AGTCATCTTT TGTTTCAGAT 
AATAGCTTAA ACAGGTCTCC TAGATCAGAA AATGAAAAGC CGGGTCTCAG CTCCAGATCT 
GTATCCATGA TTTCTGTTGC TAGTTCAGAA GATTCCTGCC ATACTACAGT GACAACCCCA 
AGGCCTCCAG TTGAGTATGA CTCTGACTTT ATGTTAGAGA GTTCAGAATC CCAAATGTCC 
TTTTCCCAGT CACCTTTTTT GTCAATTGCC AAATCTCCTG CTCTTCATGA AAGGGAATTG 
GACAGCCTGG CTGACTTGCC GGAGCGGATT AAACCACCAT ATGCAAACAG ACTTTCAACA 
TCCCATCTTA GGTCATCTTC TGTAGAAGAT GTTAAACTAA TTATAAGCGA GGGGAGACCT 
ACCATAGAAG TTCGAAGATG TAGCATGCCT TCTGTCATTT GTGAACATAC CAAACAATTC 
CAAACAATAT CAGAAGAGAG CAATCAAGGT AGCTTATTAA CTGTGCCAGG AGATACTAGT 
CCTTCTCCCA AACCTGAGGT ATTCTCAAAT GTGCCTGAAA GAGACCTTTC AAATGTATCT 
AACATACATT CCAGTTTTGC AACTTCTCCA ACTGGAGCTT CAAACAGCAA GTATGTTTCA 
GCTGATAGAA ATCTCATCAA GAATACTGCC CCAGTGAACA CTGTAATGGA CAGTCCAGTG 
CATTTAGAGC CATCTAGTCA GGTTGGTGTG ATCCAGAATA AATCATGGGA GATGCCTGTT 
GATAGACTAG AGACATTAAG CACCAGAGAC TTTATCTGCC CAAATTCTAA CATACCTGAT 
CAAGAATCCT CTCTTCAGAG TTTTTGTAAT TCTGAAAATA AGGTATTGAA AGAAAATGCT 
GATTTTTTAT CCCTGCGCCA GACTGAACTG CCAGGAAACT CTTGTGCTCA GGATCCGGCA 
TCCTTTATGC CTCCACAGCA GCCTTGCTCT TTCCCCAGCC AATCACTTTC AGATGCTGAA 
TCGATTTCTA AACATATGTC TTTGTCATAT GTTGCTAATC AAGAGCCAGG TATTTTACAA 
CAAAAAAATG CAGTTCAGAT TATTAGTTCT GCTTTAGATA CTGATAATGA ATCTACAAAA 
GATACAGAAA ATACTTTTGT CCTAGGAGAT GTTCAAAAAA CAGATGCCTT TGTCCCAGTG 
TACTCTGACA GCACTATTCA AGAAGCATCA CCAAACTTTG AGAAAGCTTA TACTTTACCT 
GTGTTACCAT CAGAAAAGGA CTTTAATGGA AGTGATGCCT CTACCCAGCT AAATACACAT 
TATGCATTTA GCAAACTAAC TTACAAGTCT TCCAGTGGCC ATGAAGTTGA GAATAGCACA 
ACTGATACTC AGGTCATTTC ACATGAAAAA GAAAACAAAC TGGAGAGTTT GGTTTTAACT 
CATTTGAGTA GGTGTGATTC TGATTTATGT GAAATGAATG CAGGGATGCC AAAAGGAAAC 
CTAAATGAAC AAGATCCAAA ACATTGTCCT GAAAGTGAAA AGTGTTTGCT TTCCATAGAA 
GATGAGGAAT CTCAACAAAG CATTTTATCA AGTCTGGAAA ACCATTCACA GCAGTCAACT 
CAACCAGAAA TGCATAAATA TGGTCAGTTA GTTAAAGTAG AATTAGAAGA AAATGCCGAA 
GATGATAAAA CTGAAAACCA AATCCCTCAA AGAATGACTA GAAACAAAGC AAATACAATG 
GCAAATCAAA GCAAACAGAT TCTTGCTAGC TGTACACTAT TATCAGAAAA AGACAGTGAA 
TCCTCATCTC CTAGAGGAAG AATAAGATTA ACTGAAGATG ACGATCCTCA AATTCACCAT 
CCACGGAAAA GGAAAGTGTC ACGTGTACCT CAGCCTGTGC AAGTGAGTCC CTCTTTACTA 
CAAGCAAAAG AGAAAACTCA GCAATCTCTG GCAGCCATTG TAGATTCTCT AAAACTAGAT 
GAGATTCAGC CATACAGTTC AGAGAGAGCA AATCCATATT TTGAATACTT GCACATAAGG 
AAAAAAATAG AAGAAAAACG CAAATTACTG TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC 
TATGACGAAT ATGTAACATT TAACGGATCA TATCTCCTGG ATGGAAACCC CTTAAGCAAG 
ATTTGTATTC CCACAATTAC ACCACCACCT TCACTGTCAG ATCCACTTAA AGAGCTTTTT 
CGACAACAGG AAGTTGTAAG GATGAAACTA CGTTTGCAAC ACAGTATTGA AAGGGAAAAA 
CTCATTGTAT CCAACGAACA AGAAGTTTTG CGAGTTCATT ACAGAGCTGC AAGAACATTG 
GCAAATCAAA CACTGCCATT TAGTGCTTGT ACTGTTTTGC TGGATGCCGA AGTATACAAT 
GTACCATTGG ACTCTCAGTC TGATGACAGT AAAACTTCTG TGAGGGATCG CTTTAATGCA 
AGACAATTCA TGTCTTGGTT ACAAGATGTG GATGATAAAT TTGACAAATT AAAGACCTGT 
CTTTTAATGA GGCAACAACA TGAAGCTGCG GCTTTAAATG CTGTCCAGAG GTTAGAATGG 
CAGCTCAAAC TCCAGGAACT TGATCCTGCC ACCTATAAAT CTATCAGCAT TTACGAAATC 
CAGGAGTTTT ATGTTCCCCT TGTTGATGTT AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881152.1
CDS	350..6469
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025663.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025663.2

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAGTCC AGATTCCACA CCAAATCATC CATCACAAAC AACGCCTGCC 
CAAAAGAAAA CTCCCAGTTC TTCATCTCGA CAGAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCTGCT ATTCGAGGAG ATGTGAAACA AGTTAAAGAA 
TTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ATGTTGGATA TTACGATGTT GCTAAGATAC TTATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCTGCTAG TAGTGGGCAC 
AGAGATATAG TAAAGCTGTT ACTTCGTCAC GGTGGAAATC CATTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTGGATGT AGCAGAAACA GAGGAGTTGG AGTTGCTACT AAAAAGAGAG 
GTGCCTTTAT CTGATGATGA TGAAAGTTAC ACAGATTCCG AAGAGGCTCA ATCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCT GAAACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TACTTCCCAG TAAAACACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGATGAAGA AATTAATAAG ATGATTGATG ATAGGCATAT TCTTAGGAAA 
GAACAACGAA AAGAAAATGA ACCTGAAGCA GAAAAAACTC ATTTATTTGC AAAACAGGAG 
AAAGCCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTCTTATAT 
TCAAGTACTG AAAGTTCTGA TGAAGAAGCT CTTCAGAATA AAAAGATTTC TACTTCATGT 
TCCGTCATCC CTGAAACATC AAATTCTGAT ATGCAAACCA AAAAGGAATA TGTAGTTTCA 
GGTGAACACA AACAGAAAGG CAAAGTTAAA AGAAAATTGA AAAATCAGAA TAAAAATAAA 
GAGAACCAAG AGCTAAAGCA AGAAAAGGAA GGAAAAGAAA ATACAAGAAT AACAAACTTG 
ACAGTAAATA CTGGACTAGA TTGTTCAGAA AAGACCAGAG AGGAGGGGAA CTTTAGGAAA 
TCTTTTAGCC CAAAAGATGA TACTTCATTA CATTTATTTC ATATTTCCAC TGGTAAATCT 
CCCAAACATT CTTGTGGATT AAGTGAAAAA CAGTCAACAC CACTAAAACA AGAACATACT 
AAAACATGTT TATCACCAGG AAGTTCTGAA ATGTCATTAC AGCCTGATCT TGTTCGGTAT 
GATAATACAG AATCTGAATT CTTGCCAGAA AGTTCAAGTG TAAAATCTTG TAAGCATAAG 
GAAAAAAGCA AACATCAGAA AGATTTCCAC TTAGAATTTG GTGAAAAATC AAATGCCAAA 
ATAAAGGATG AAGATCATAG TCCAACATTT GAAAATTCAG ATTGCACACT GAAAAAAATG 
GATAAAGAAG GTAAAACATT AAAAAAACAT AAATTGAAGC ATAAAGAGAG GGAAAAAGAA 
AAGCATAAAA AAGAAATTGA AGGTGAAAAG GAAAAATACA AAACTAAGGA TAGTGCCAAA 
GAACTGCAGA GGAGTGTGGA ATTTGATAGA GAATTTTGGA AAGAGAATTT TTTTAAAAGT 
GATGAAACTG AAGATCTCTT TTTAAATATG GAACATGAAT CCTTAACATT AGAAAAAAAA 
TCAAAATTGG AAAAAAACAT CAAAGATGAT AAATCAACCA AGGAAAAGCA TGTGTCAAAA 
GAGAGGAACT TTAAAGAGGA ACGAGACAAG ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG 
GAGGAAAAAA TAAAAGATCT AAAAGAAGAG AGAGAAAACA TACCCACAGA TAAAGACTCA 
GAATTTACTT CTTTGGGTAT GAGTGCCATT GAGGAATCTA TAGGGCTTCA TTTAGTGGAA 
AAGGAAATAG ACATTGAAAA ACAAGAAAAG CATATAAAGG AAAGTAAAGA AAAACCTGAG 
AAGCGATCTC AAATTAAAGA AAAGGACATT GAGAAGATGG AAAGAAAAAC CTTTGAAAAA 
GAAAAGAAGA TAAAACATGA GCATAAGTCA GAAAAAGACA AATTAGATCT TAGTGAATGT 
GTTGATAAAA TAAAAGAAAA GGACAAGCTA TATTCGCATC ACACAGAAAA ATGCCATAAA 
GAAGGTGAGA AGAGCAAAAA TACTGCTGCT ATTAAAAAAA CTGACGACAG AGAGAAAAGT 
AGAGAAAAGA TGGATAGGAA ACATGACAAA GAAAAGCCTG AAAAAGAGAG GCATCTAGCA 
GAAAGCAAAG AAAAGCACTT GATGGAGAAA AAAAATAAAC AATCAGATAA TAGTGAATAC 
AGTAAATCAG AAAAAGGCAA AAATAAAGAA AAAGACAGGG AGCTAGATAA AAAGGAAAAA 
TCTAGAGATA AAGAAAGTAT AAATATAACT AACTCCAAAC ACATACAGGA AGAAAAAAAA 
TCAAGTATAG TAGACGGTAA TAAAGCACAA CATGAAAAAC CCTTATCCCT TAAAGAAAAA 
ACAAAAGATG AACCTTTGAA AACTCCAGAT GGAAAAGAAA AAGATAAAAA AGATAAAGAT 
ATAGATAGAT ACAAAGAACG AGACAAACAT AAAGATAAAA TTCAAATAAA TAGCTTACTC 
AAACTAAAAT CTGAAGCAGA TAAGCCTAAA CCTAAGTCAT CACCAGCATC AAAAGATACC 
CGACCTAAAG AAAAGAGGTT AGTGAATGAT GATTTAATGC AGACAAGTTT TGAACGAATG 
CTAAGCCTTA AAGACCTAGA AATAGAACAG TGGCACAAAA AACATAAGGA AAAAATTAAG 
CAAAAAGAAA AGGAACGGTT GAGAAACCGA AACTGTTTAG AACTTAAAAT AAAAGATAAA 
GAAAAAACAA AGCATACACC AACTGAATCC AAAAATAAAG AACTTACTAG GTCAAAGAGT 
TCAGAAGTGA CTGATGCATA TACCAAGGAG AAACAACCTA AAGATGCTGT AAGTAACAGA 
TCACAATCTG TTGACACCAA AAATGTAATG ACTTTAGGGA AGTCATCTTT TGTTTCAGAT 
AATAGCTTAA ACAGGTCTCC TAGATCAGAA AATGAAAAGC CGGGTCTCAG CTCCAGATCT 
GTATCCATGA TTTCTGTTGC TAGTTCAGAA GATTCCTGCC ATACTACAGT GACAACCCCA 
AGGCCTCCAG TTGAGTATGA CTCTGACTTT ATGTTAGAGA GTTCAGAATC CCAAATGTCC 
TTTTCCCAGT CACCTTTTTT GTCAATTGCC AAATCTCCTG CTCTTCATGA AAGGGAATTG 
GACAGCCTGG CTGACTTGCC GGAGCGGATT AAACCACCAT ATGCAAACAG ACTTTCAACA 
TCCCATCTTA GGTCATCTTC TGTAGAAGAT GTTAAACTAA TTATAAGCGA GGGGAGACCT 
ACCATAGAAG TTCGAAGATG TAGCATGCCT TCTGTCATTT GTGAACATAC CAAACAATTC 
CAAACAATAT CAGAAGAGAG CAATCAAGGT AGCTTATTAA CTGTGCCAGG AGATACTAGT 
CCTTCTCCCA AACCTGAGGT ATTCTCAAAT GTGCCTGAAA GAGACCTTTC AAATGTATCT 
AACATACATT CCAGTTTTGC AACTTCTCCA ACTGGAGCTT CAAACAGCAA GTATGTTTCA 
GCTGATAGAA ATCTCATCAA GAATACTGCC CCAGTGAACA CTGTAATGGA CAGTCCAGTG 
CATTTAGAGC CATCTAGTCA GGTTGGTGTG ATCCAGAATA AATCATGGGA GATGCCTGTT 
GATAGACTAG AGACATTAAG CACCAGAGAC TTTATCTGCC CAAATTCTAA CATACCTGAT 
CAAGAATCCT CTCTTCAGAG TTTTTGTAAT TCTGAAAATA AGGTATTGAA AGAAAATGCT 
GATTTTTTAT CCCTGCGCCA GACTGAACTG CCAGGAAACT CTTGTGCTCA GGATCCGGCA 
TCCTTTATGC CTCCACAGCA GCCTTGCTCT TTCCCCAGCC AATCACTTTC AGATGCTGAA 
TCGATTTCTA AACATATGTC TTTGTCATAT GTTGCTAATC AAGAGCCAGG TATTTTACAA 
CAAAAAAATG CAGTTCAGAT TATTAGTTCT GCTTTAGATA CTGATAATGA ATCTACAAAA 
GATACAGAAA ATACTTTTGT CCTAGGAGAT GTTCAAAAAA CAGATGCCTT TGTCCCAGTG 
TACTCTGACA GCACTATTCA AGAAGCATCA CCAAACTTTG AGAAAGCTTA TACTTTACCT 
GTGTTACCAT CAGAAAAGGA CTTTAATGGA AGTGATGCCT CTACCCAGCT AAATACACAT 
TATGCATTTA GCAAACTAAC TTACAAGTCT TCCAGTGGCC ATGAAGTTGA GAATAGCACA 
ACTGATACTC AGGTCATTTC ACATGAAAAA GAAAACAAAC TGGAGAGTTT GGTTTTAACT 
CATTTGAGTA GGTGTGATTC TGATTTATGT GAAATGAATG CAGGGATGCC AAAAGGAAAC 
CTAAATGAAC AAGATCCAAA ACATTGTCCT GAAAGTGAAA AGTGTTTGCT TTCCATAGAA 
GATGAGGAAT CTCAACAAAG CATTTTATCA AGTCTGGAAA ACCATTCACA GCAGTCAACT 
CAACCAGAAA TGCATAAATA TGGTCAGTTA GTTAAAGTAG AATTAGAAGA AAATGCCGAA 
GATGATAAAA CTGAAAACCA AATCCCTCAA AGAATGACTA GAAACAAAGC AAATACAATG 
GCAAATCAAA GCAAACAGAT TCTTGCTAGC TGTACACTAT TATCAGAAAA AGACAGTGAA 
TCCTCATCTC CTAGAGGAAG AATAAGATTA ACTGAAGATG ACGATCCTCA AATTCACCAT 
CCACGGAAAA GGAAAGTGTC ACGTGTACCT CAGCCTGTGC AAGTGAGTCC CTCTTTACTA 
CAAGCAAAAG AGAAAACTCA GCAATCTCTG GCAGCCATTG TAGATTCTCT AAAACTAGAT 
GAGATTCAGC CATACAGTTC AGAGAGAGCA AATCCATATT TTGAATACTT GCACATAAGG 
AAAAAAATAG AAGAAAAACG CAAATTACTG TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC 
TATGACGAAT ATGTAACATT TAACGGATCA TATCTCCTGG ATGGAAACCC CTTAAGCAAG 
ATTTGTATTC CCACAATTAC ACCACCACCT TCACTGTCAG ATCCACTTAA AGAGCTTTTT 
CGACAACAGG AAGTTGTAAG GATGAAACTA CGTTTGCAAC ACAGTATTGA AAGGGAAAAA 
CTCATTGTAT CCAACGAACA AGAAGTTTTG CGAGTTCATT ACAGAGCTGC AAGAACATTG 
GCAAATCAAA CACTGCCATT TAGTGCTTGT ACTGTTTTGC TGGATGCCGA AGTATACAAT 
GTACCATTGG ACTCTCAGTC TGATGACAGT AAAACTTCTG TGAGGGATCG CTTTAATGCA 
AGACAATTCA TGTCTTGGTT ACAAGATGTG GATGATAAAT TTGACAAATT AAAGACCTGT 
CTTTTAATGA GGCAACAACA TGAAGCTGCG GCTTTAAATG CTGTCCAGAG GTTAGAATGG 
CAGCTCAAAC TCCAGGAACT TGATCCTGCC ACCTATAAAT CTATCAGCAT TTACGAAATC 
CAGGAGTTTT ATGTTCCCCT TGTTGATGTT AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_005258095.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258152.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X4, mRNA.

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CloneID	OHu50996
Clone ID Related Accession (Same CDS sequence)	XM_005258095.3 , XM_017025662.1 , XM_017025661.1 , XM_017025661.2 , XM_017025662.2 , XM_005258095.2
Accession Version	XM_005258095.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005258095.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAATTCC ACACCAAATC ATCCATCACA AACAACGCCT 
GCCCAAAAGA AAACTCCCAG TTCTTCATCT CGACAGAAAG ATAAAGTTAA TAAAAGAAAT 
GAACGTGGTG AAACTCCTTT ACACATGGCT GCTATTCGAG GAGATGTGAA ACAAGTTAAA 
GAATTAATAA GTTTAGGGGC AAATGTGAAT GTGAAAGATT TTGCAGGTTG GACACCACTG 
CATGAAGCTT GCAATGTTGG ATATTACGAT GTTGCTAAGA TACTTATAGC AGCTGGAGCA 
GATGTTAACA CACAAGGATT AGATGATGAC ACTCCACTCC ATGATTCTGC TAGTAGTGGG 
CACAGAGATA TAGTAAAGCT GTTACTTCGT CACGGTGGAA ATCCATTTCA AGCTAATAAA 
CATGGGGAGC GTCCAGTGGA TGTAGCAGAA ACAGAGGAGT TGGAGTTGCT ACTAAAAAGA 
GAGGTGCCTT TATCTGATGA TGATGAAAGT TACACAGATT CCGAAGAGGC TCAATCTGTA 
AATCCTTCTA GTGTTGATGA AAATATTGAC TCTGAAACAG AGAAAGACTC TCTCATCTGT 
GAAAGTAAAC AGATACTTCC CAGTAAAACA CCTCTTCCAT CTGCCCTTGA TGAGTATGAG 
TTCAAAGATG ATGATGATGA AGAAATTAAT AAGATGATTG ATGATAGGCA TATTCTTAGG 
AAAGAACAAC GAAAAGAAAA TGAACCTGAA GCAGAAAAAA CTCATTTATT TGCAAAACAG 
GAGAAAGCCT TCTATCCTAA ATCATTTAAA AGTAAAAAAC AAAAGCCATC TAGGGTCTTA 
TATTCAAGTA CTGAAAGTTC TGATGAAGAA GCTCTTCAGA ATAAAAAGAT TTCTACTTCA 
TGTTCCGTCA TCCCTGAAAC ATCAAATTCT GATATGCAAA CCAAAAAGGA ATATGTAGTT 
TCAGGTGAAC ACAAACAGAA AGGCAAAGTT AAAAGAAAAT TGAAAAATCA GAATAAAAAT 
AAAGAGAACC AAGAGCTAAA GCAAGAAAAG GAAGGAAAAG AAAATACAAG AATAACAAAC 
TTGACAGTAA ATACTGGACT AGATTGTTCA GAAAAGACCA GAGAGGAGGG GAACTTTAGG 
AAATCTTTTA GCCCAAAAGA TGATACTTCA TTACATTTAT TTCATATTTC CACTGGTAAA 
TCTCCCAAAC ATTCTTGTGG ATTAAGTGAA AAACAGTCAA CACCACTAAA ACAAGAACAT 
ACTAAAACAT GTTTATCACC AGGAAGTTCT GAAATGTCAT TACAGCCTGA TCTTGTTCGG 
TATGATAATA CAGAATCTGA ATTCTTGCCA GAAAGTTCAA GTGTAAAATC TTGTAAGCAT 
AAGGAAAAAA GCAAACATCA GAAAGATTTC CACTTAGAAT TTGGTGAAAA ATCAAATGCC 
AAAATAAAGG ATGAAGATCA TAGTCCAACA TTTGAAAATT CAGATTGCAC ACTGAAAAAA 
ATGGATAAAG AAGGTAAAAC ATTAAAAAAA CATAAATTGA AGCATAAAGA GAGGGAAAAA 
GAAAAGCATA AAAAAGAAAT TGAAGGTGAA AAGGAAAAAT ACAAAACTAA GGATAGTGCC 
AAAGAACTGC AGAGGAGTGT GGAATTTGAT AGAGAATTTT GGAAAGAGAA TTTTTTTAAA 
AGTGATGAAA CTGAAGATCT CTTTTTAAAT ATGGAACATG AATCCTTAAC ATTAGAAAAA 
AAATCAAAAT TGGAAAAAAA CATCAAAGAT GATAAATCAA CCAAGGAAAA GCATGTGTCA 
AAAGAGAGGA ACTTTAAAGA GGAACGAGAC AAGATTAAAA AGGAAAGCGA GAAATCTTTT 
AGGGAGGAAA AAATAAAAGA TCTAAAAGAA GAGAGAGAAA ACATACCCAC AGATAAAGAC 
TCAGAATTTA CTTCTTTGGG TATGAGTGCC ATTGAGGAAT CTATAGGGCT TCATTTAGTG 
GAAAAGGAAA TAGACATTGA AAAACAAGAA AAGCATATAA AGGAAAGTAA AGAAAAACCT 
GAGAAGCGAT CTCAAATTAA AGAAAAGGAC ATTGAGAAGA TGGAAAGAAA AACCTTTGAA 
AAAGAAAAGA AGATAAAACA TGAGCATAAG TCAGAAAAAG ACAAATTAGA TCTTAGTGAA 
TGTGTTGATA AAATAAAAGA AAAGGACAAG CTATATTCGC ATCACACAGA AAAATGCCAT 
AAAGAAGGTG AGAAGAGCAA AAATACTGCT GCTATTAAAA AAACTGACGA CAGAGAGAAA 
AGTAGAGAAA AGATGGATAG GAAACATGAC AAAGAAAAGC CTGAAAAAGA GAGGCATCTA 
GCAGAAAGCA AAGAAAAGCA CTTGATGGAG AAAAAAAATA AACAATCAGA TAATAGTGAA 
TACAGTAAAT CAGAAAAAGG CAAAAATAAA GAAAAAGACA GGGAGCTAGA TAAAAAGGAA 
AAATCTAGAG ATAAAGAAAG TATAAATATA ACTAACTCCA AACACATACA GGAAGAAAAA 
AAATCAAGTA TAGTAGACGG TAATAAAGCA CAACATGAAA AACCCTTATC CCTTAAAGAA 
AAAACAAAAG ATGAACCTTT GAAAACTCCA GATGGAAAAG AAAAAGATAA AAAAGATAAA 
GATATAGATA GATACAAAGA ACGAGACAAA CATAAAGATA AAATTCAAAT AAATAGCTTA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCTAAGT CATCACCAGC ATCAAAAGAT 
ACCCGACCTA AAGAAAAGAG GTTAGTGAAT GATGATTTAA TGCAGACAAG TTTTGAACGA 
ATGCTAAGCC TTAAAGACCT AGAAATAGAA CAGTGGCACA AAAAACATAA GGAAAAAATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAC CGAAACTGTT TAGAACTTAA AATAAAAGAT 
AAAGAAAAAA CAAAGCATAC ACCAACTGAA TCCAAAAATA AAGAACTTAC TAGGTCAAAG 
AGTTCAGAAG TGACTGATGC ATATACCAAG GAGAAACAAC CTAAAGATGC TGTAAGTAAC 
AGATCACAAT CTGTTGACAC CAAAAATGTA ATGACTTTAG GGAAGTCATC TTTTGTTTCA 
GATAATAGCT TAAACAGGTC TCCTAGATCA GAAAATGAAA AGCCGGGTCT CAGCTCCAGA 
TCTGTATCCA TGATTTCTGT TGCTAGTTCA GAAGATTCCT GCCATACTAC AGTGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCTGAC TTTATGTTAG AGAGTTCAGA ATCCCAAATG 
TCCTTTTCCC AGTCACCTTT TTTGTCAATT GCCAAATCTC CTGCTCTTCA TGAAAGGGAA 
TTGGACAGCC TGGCTGACTT GCCGGAGCGG ATTAAACCAC CATATGCAAA CAGACTTTCA 
ACATCCCATC TTAGGTCATC TTCTGTAGAA GATGTTAAAC TAATTATAAG CGAGGGGAGA 
CCTACCATAG AAGTTCGAAG ATGTAGCATG CCTTCTGTCA TTTGTGAACA TACCAAACAA 
TTCCAAACAA TATCAGAAGA GAGCAATCAA GGTAGCTTAT TAACTGTGCC AGGAGATACT 
AGTCCTTCTC CCAAACCTGA GGTATTCTCA AATGTGCCTG AAAGAGACCT TTCAAATGTA 
TCTAACATAC ATTCCAGTTT TGCAACTTCT CCAACTGGAG CTTCAAACAG CAAGTATGTT 
TCAGCTGATA GAAATCTCAT CAAGAATACT GCCCCAGTGA ACACTGTAAT GGACAGTCCA 
GTGCATTTAG AGCCATCTAG TCAGGTTGGT GTGATCCAGA ATAAATCATG GGAGATGCCT 
GTTGATAGAC TAGAGACATT AAGCACCAGA GACTTTATCT GCCCAAATTC TAACATACCT 
GATCAAGAAT CCTCTCTTCA GAGTTTTTGT AATTCTGAAA ATAAGGTATT GAAAGAAAAT 
GCTGATTTTT TATCCCTGCG CCAGACTGAA CTGCCAGGAA ACTCTTGTGC TCAGGATCCG 
GCATCCTTTA TGCCTCCACA GCAGCCTTGC TCTTTCCCCA GCCAATCACT TTCAGATGCT 
GAATCGATTT CTAAACATAT GTCTTTGTCA TATGTTGCTA ATCAAGAGCC AGGTATTTTA 
CAACAAAAAA ATGCAGTTCA GATTATTAGT TCTGCTTTAG ATACTGATAA TGAATCTACA 
AAAGATACAG AAAATACTTT TGTCCTAGGA GATGTTCAAA AAACAGATGC CTTTGTCCCA 
GTGTACTCTG ACAGCACTAT TCAAGAAGCA TCACCAAACT TTGAGAAAGC TTATACTTTA 
CCTGTGTTAC CATCAGAAAA GGACTTTAAT GGAAGTGATG CCTCTACCCA GCTAAATACA 
CATTATGCAT TTAGCAAACT AACTTACAAG TCTTCCAGTG GCCATGAAGT TGAGAATAGC 
ACAACTGATA CTCAGGTCAT TTCACATGAA AAAGAAAACA AACTGGAGAG TTTGGTTTTA 
ACTCATTTGA GTAGGTGTGA TTCTGATTTA TGTGAAATGA ATGCAGGGAT GCCAAAAGGA 
AACCTAAATG AACAAGATCC AAAACATTGT CCTGAAAGTG AAAAGTGTTT GCTTTCCATA 
GAAGATGAGG AATCTCAACA AAGCATTTTA TCAAGTCTGG AAAACCATTC ACAGCAGTCA 
ACTCAACCAG AAATGCATAA ATATGGTCAG TTAGTTAAAG TAGAATTAGA AGAAAATGCC 
GAAGATGATA AAACTGAAAA CCAAATCCCT CAAAGAATGA CTAGAAACAA AGCAAATACA 
ATGGCAAATC AAAGCAAACA GATTCTTGCT AGCTGTACAC TATTATCAGA AAAAGACAGT 
GAATCCTCAT CTCCTAGAGG AAGAATAAGA TTAACTGAAG ATGACGATCC TCAAATTCAC 
CATCCACGGA AAAGGAAAGT GTCACGTGTA CCTCAGCCTG TGCAAGTGAG TCCCTCTTTA 
CTACAAGCAA AAGAGAAAAC TCAGCAATCT CTGGCAGCCA TTGTAGATTC TCTAAAACTA 
GATGAGATTC AGCCATACAG TTCAGAGAGA GCAAATCCAT ATTTTGAATA CTTGCACATA 
AGGAAAAAAA TAGAAGAAAA ACGCAAATTA CTGTGTAGTG TGATTCCTCA AGCACCTCAG 
TACTATGACG AATATGTAAC ATTTAACGGA TCATATCTCC TGGATGGAAA CCCCTTAAGC 
AAGATTTGTA TTCCCACAAT TACACCACCA CCTTCACTGT CAGATCCACT TAAAGAGCTT 
TTTCGACAAC AGGAAGTTGT AAGGATGAAA CTACGTTTGC AACACAGTAT TGAAAGGGAA 
AAACTCATTG TATCCAACGA ACAAGAAGTT TTGCGAGTTC ATTACAGAGC TGCAAGAACA 
TTGGCAAATC AAACACTGCC ATTTAGTGCT TGTACTGTTT TGCTGGATGC CGAAGTATAC 
AATGTACCAT TGGACTCTCA GTCTGATGAC AGTAAAACTT CTGTGAGGGA TCGCTTTAAT 
GCAAGACAAT TCATGTCTTG GTTACAAGAT GTGGATGATA AATTTGACAA ATTAAAGACC 
TGTCTTTTAA TGAGGCAACA ACATGAAGCT GCGGCTTTAA ATGCTGTCCA GAGGTTAGAA 
TGGCAGCTCA AACTCCAGGA ACTTGATCCT GCCACCTATA AATCTATCAG CATTTACGAA 
ATCCAGGAGT TTTATGTTCC CCTTGTTGAT GTTAACGACG ACTTTGAATT GACTCCTATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258152.1
CDS	241..6423
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNNSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_005258095.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005258095.3

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAATTCC ACACCAAATC ATCCATCACA AACAACGCCT 
GCCCAAAAGA AAACTCCCAG TTCTTCATCT CGACAGAAAG ATAAAGTTAA TAAAAGAAAT 
GAACGTGGTG AAACTCCTTT ACACATGGCT GCTATTCGAG GAGATGTGAA ACAAGTTAAA 
GAATTAATAA GTTTAGGGGC AAATGTGAAT GTGAAAGATT TTGCAGGTTG GACACCACTG 
CATGAAGCTT GCAATGTTGG ATATTACGAT GTTGCTAAGA TACTTATAGC AGCTGGAGCA 
GATGTTAACA CACAAGGATT AGATGATGAC ACTCCACTCC ATGATTCTGC TAGTAGTGGG 
CACAGAGATA TAGTAAAGCT GTTACTTCGT CACGGTGGAA ATCCATTTCA AGCTAATAAA 
CATGGGGAGC GTCCAGTGGA TGTAGCAGAA ACAGAGGAGT TGGAGTTGCT ACTAAAAAGA 
GAGGTGCCTT TATCTGATGA TGATGAAAGT TACACAGATT CCGAAGAGGC TCAATCTGTA 
AATCCTTCTA GTGTTGATGA AAATATTGAC TCTGAAACAG AGAAAGACTC TCTCATCTGT 
GAAAGTAAAC AGATACTTCC CAGTAAAACA CCTCTTCCAT CTGCCCTTGA TGAGTATGAG 
TTCAAAGATG ATGATGATGA AGAAATTAAT AAGATGATTG ATGATAGGCA TATTCTTAGG 
AAAGAACAAC GAAAAGAAAA TGAACCTGAA GCAGAAAAAA CTCATTTATT TGCAAAACAG 
GAGAAAGCCT TCTATCCTAA ATCATTTAAA AGTAAAAAAC AAAAGCCATC TAGGGTCTTA 
TATTCAAGTA CTGAAAGTTC TGATGAAGAA GCTCTTCAGA ATAAAAAGAT TTCTACTTCA 
TGTTCCGTCA TCCCTGAAAC ATCAAATTCT GATATGCAAA CCAAAAAGGA ATATGTAGTT 
TCAGGTGAAC ACAAACAGAA AGGCAAAGTT AAAAGAAAAT TGAAAAATCA GAATAAAAAT 
AAAGAGAACC AAGAGCTAAA GCAAGAAAAG GAAGGAAAAG AAAATACAAG AATAACAAAC 
TTGACAGTAA ATACTGGACT AGATTGTTCA GAAAAGACCA GAGAGGAGGG GAACTTTAGG 
AAATCTTTTA GCCCAAAAGA TGATACTTCA TTACATTTAT TTCATATTTC CACTGGTAAA 
TCTCCCAAAC ATTCTTGTGG ATTAAGTGAA AAACAGTCAA CACCACTAAA ACAAGAACAT 
ACTAAAACAT GTTTATCACC AGGAAGTTCT GAAATGTCAT TACAGCCTGA TCTTGTTCGG 
TATGATAATA CAGAATCTGA ATTCTTGCCA GAAAGTTCAA GTGTAAAATC TTGTAAGCAT 
AAGGAAAAAA GCAAACATCA GAAAGATTTC CACTTAGAAT TTGGTGAAAA ATCAAATGCC 
AAAATAAAGG ATGAAGATCA TAGTCCAACA TTTGAAAATT CAGATTGCAC ACTGAAAAAA 
ATGGATAAAG AAGGTAAAAC ATTAAAAAAA CATAAATTGA AGCATAAAGA GAGGGAAAAA 
GAAAAGCATA AAAAAGAAAT TGAAGGTGAA AAGGAAAAAT ACAAAACTAA GGATAGTGCC 
AAAGAACTGC AGAGGAGTGT GGAATTTGAT AGAGAATTTT GGAAAGAGAA TTTTTTTAAA 
AGTGATGAAA CTGAAGATCT CTTTTTAAAT ATGGAACATG AATCCTTAAC ATTAGAAAAA 
AAATCAAAAT TGGAAAAAAA CATCAAAGAT GATAAATCAA CCAAGGAAAA GCATGTGTCA 
AAAGAGAGGA ACTTTAAAGA GGAACGAGAC AAGATTAAAA AGGAAAGCGA GAAATCTTTT 
AGGGAGGAAA AAATAAAAGA TCTAAAAGAA GAGAGAGAAA ACATACCCAC AGATAAAGAC 
TCAGAATTTA CTTCTTTGGG TATGAGTGCC ATTGAGGAAT CTATAGGGCT TCATTTAGTG 
GAAAAGGAAA TAGACATTGA AAAACAAGAA AAGCATATAA AGGAAAGTAA AGAAAAACCT 
GAGAAGCGAT CTCAAATTAA AGAAAAGGAC ATTGAGAAGA TGGAAAGAAA AACCTTTGAA 
AAAGAAAAGA AGATAAAACA TGAGCATAAG TCAGAAAAAG ACAAATTAGA TCTTAGTGAA 
TGTGTTGATA AAATAAAAGA AAAGGACAAG CTATATTCGC ATCACACAGA AAAATGCCAT 
AAAGAAGGTG AGAAGAGCAA AAATACTGCT GCTATTAAAA AAACTGACGA CAGAGAGAAA 
AGTAGAGAAA AGATGGATAG GAAACATGAC AAAGAAAAGC CTGAAAAAGA GAGGCATCTA 
GCAGAAAGCA AAGAAAAGCA CTTGATGGAG AAAAAAAATA AACAATCAGA TAATAGTGAA 
TACAGTAAAT CAGAAAAAGG CAAAAATAAA GAAAAAGACA GGGAGCTAGA TAAAAAGGAA 
AAATCTAGAG ATAAAGAAAG TATAAATATA ACTAACTCCA AACACATACA GGAAGAAAAA 
AAATCAAGTA TAGTAGACGG TAATAAAGCA CAACATGAAA AACCCTTATC CCTTAAAGAA 
AAAACAAAAG ATGAACCTTT GAAAACTCCA GATGGAAAAG AAAAAGATAA AAAAGATAAA 
GATATAGATA GATACAAAGA ACGAGACAAA CATAAAGATA AAATTCAAAT AAATAGCTTA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCTAAGT CATCACCAGC ATCAAAAGAT 
ACCCGACCTA AAGAAAAGAG GTTAGTGAAT GATGATTTAA TGCAGACAAG TTTTGAACGA 
ATGCTAAGCC TTAAAGACCT AGAAATAGAA CAGTGGCACA AAAAACATAA GGAAAAAATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAC CGAAACTGTT TAGAACTTAA AATAAAAGAT 
AAAGAAAAAA CAAAGCATAC ACCAACTGAA TCCAAAAATA AAGAACTTAC TAGGTCAAAG 
AGTTCAGAAG TGACTGATGC ATATACCAAG GAGAAACAAC CTAAAGATGC TGTAAGTAAC 
AGATCACAAT CTGTTGACAC CAAAAATGTA ATGACTTTAG GGAAGTCATC TTTTGTTTCA 
GATAATAGCT TAAACAGGTC TCCTAGATCA GAAAATGAAA AGCCGGGTCT CAGCTCCAGA 
TCTGTATCCA TGATTTCTGT TGCTAGTTCA GAAGATTCCT GCCATACTAC AGTGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCTGAC TTTATGTTAG AGAGTTCAGA ATCCCAAATG 
TCCTTTTCCC AGTCACCTTT TTTGTCAATT GCCAAATCTC CTGCTCTTCA TGAAAGGGAA 
TTGGACAGCC TGGCTGACTT GCCGGAGCGG ATTAAACCAC CATATGCAAA CAGACTTTCA 
ACATCCCATC TTAGGTCATC TTCTGTAGAA GATGTTAAAC TAATTATAAG CGAGGGGAGA 
CCTACCATAG AAGTTCGAAG ATGTAGCATG CCTTCTGTCA TTTGTGAACA TACCAAACAA 
TTCCAAACAA TATCAGAAGA GAGCAATCAA GGTAGCTTAT TAACTGTGCC AGGAGATACT 
AGTCCTTCTC CCAAACCTGA GGTATTCTCA AATGTGCCTG AAAGAGACCT TTCAAATGTA 
TCTAACATAC ATTCCAGTTT TGCAACTTCT CCAACTGGAG CTTCAAACAG CAAGTATGTT 
TCAGCTGATA GAAATCTCAT CAAGAATACT GCCCCAGTGA ACACTGTAAT GGACAGTCCA 
GTGCATTTAG AGCCATCTAG TCAGGTTGGT GTGATCCAGA ATAAATCATG GGAGATGCCT 
GTTGATAGAC TAGAGACATT AAGCACCAGA GACTTTATCT GCCCAAATTC TAACATACCT 
GATCAAGAAT CCTCTCTTCA GAGTTTTTGT AATTCTGAAA ATAAGGTATT GAAAGAAAAT 
GCTGATTTTT TATCCCTGCG CCAGACTGAA CTGCCAGGAA ACTCTTGTGC TCAGGATCCG 
GCATCCTTTA TGCCTCCACA GCAGCCTTGC TCTTTCCCCA GCCAATCACT TTCAGATGCT 
GAATCGATTT CTAAACATAT GTCTTTGTCA TATGTTGCTA ATCAAGAGCC AGGTATTTTA 
CAACAAAAAA ATGCAGTTCA GATTATTAGT TCTGCTTTAG ATACTGATAA TGAATCTACA 
AAAGATACAG AAAATACTTT TGTCCTAGGA GATGTTCAAA AAACAGATGC CTTTGTCCCA 
GTGTACTCTG ACAGCACTAT TCAAGAAGCA TCACCAAACT TTGAGAAAGC TTATACTTTA 
CCTGTGTTAC CATCAGAAAA GGACTTTAAT GGAAGTGATG CCTCTACCCA GCTAAATACA 
CATTATGCAT TTAGCAAACT AACTTACAAG TCTTCCAGTG GCCATGAAGT TGAGAATAGC 
ACAACTGATA CTCAGGTCAT TTCACATGAA AAAGAAAACA AACTGGAGAG TTTGGTTTTA 
ACTCATTTGA GTAGGTGTGA TTCTGATTTA TGTGAAATGA ATGCAGGGAT GCCAAAAGGA 
AACCTAAATG AACAAGATCC AAAACATTGT CCTGAAAGTG AAAAGTGTTT GCTTTCCATA 
GAAGATGAGG AATCTCAACA AAGCATTTTA TCAAGTCTGG AAAACCATTC ACAGCAGTCA 
ACTCAACCAG AAATGCATAA ATATGGTCAG TTAGTTAAAG TAGAATTAGA AGAAAATGCC 
GAAGATGATA AAACTGAAAA CCAAATCCCT CAAAGAATGA CTAGAAACAA AGCAAATACA 
ATGGCAAATC AAAGCAAACA GATTCTTGCT AGCTGTACAC TATTATCAGA AAAAGACAGT 
GAATCCTCAT CTCCTAGAGG AAGAATAAGA TTAACTGAAG ATGACGATCC TCAAATTCAC 
CATCCACGGA AAAGGAAAGT GTCACGTGTA CCTCAGCCTG TGCAAGTGAG TCCCTCTTTA 
CTACAAGCAA AAGAGAAAAC TCAGCAATCT CTGGCAGCCA TTGTAGATTC TCTAAAACTA 
GATGAGATTC AGCCATACAG TTCAGAGAGA GCAAATCCAT ATTTTGAATA CTTGCACATA 
AGGAAAAAAA TAGAAGAAAA ACGCAAATTA CTGTGTAGTG TGATTCCTCA AGCACCTCAG 
TACTATGACG AATATGTAAC ATTTAACGGA TCATATCTCC TGGATGGAAA CCCCTTAAGC 
AAGATTTGTA TTCCCACAAT TACACCACCA CCTTCACTGT CAGATCCACT TAAAGAGCTT 
TTTCGACAAC AGGAAGTTGT AAGGATGAAA CTACGTTTGC AACACAGTAT TGAAAGGGAA 
AAACTCATTG TATCCAACGA ACAAGAAGTT TTGCGAGTTC ATTACAGAGC TGCAAGAACA 
TTGGCAAATC AAACACTGCC ATTTAGTGCT TGTACTGTTT TGCTGGATGC CGAAGTATAC 
AATGTACCAT TGGACTCTCA GTCTGATGAC AGTAAAACTT CTGTGAGGGA TCGCTTTAAT 
GCAAGACAAT TCATGTCTTG GTTACAAGAT GTGGATGATA AATTTGACAA ATTAAAGACC 
TGTCTTTTAA TGAGGCAACA ACATGAAGCT GCGGCTTTAA ATGCTGTCCA GAGGTTAGAA 
TGGCAGCTCA AACTCCAGGA ACTTGATCCT GCCACCTATA AATCTATCAG CATTTACGAA 
ATCCAGGAGT TTTATGTTCC CCTTGTTGAT GTTAACGACG ACTTTGAATT GACTCCTATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025662.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881151.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X5, mRNA.

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CloneID	OHu50996
Clone ID Related Accession (Same CDS sequence)	XM_005258095.3 , XM_017025662.1 , XM_017025661.1 , XM_017025661.2 , XM_017025662.2 , XM_005258095.2
Accession Version	XM_017025662.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAATTCC ACACCAAATC ATCCATCACA AACAACGCCT 
GCCCAAAAGA AAACTCCCAG TTCTTCATCT CGACAGAAAG ATAAAGTTAA TAAAAGAAAT 
GAACGTGGTG AAACTCCTTT ACACATGGCT GCTATTCGAG GAGATGTGAA ACAAGTTAAA 
GAATTAATAA GTTTAGGGGC AAATGTGAAT GTGAAAGATT TTGCAGGTTG GACACCACTG 
CATGAAGCTT GCAATGTTGG ATATTACGAT GTTGCTAAGA TACTTATAGC AGCTGGAGCA 
GATGTTAACA CACAAGGATT AGATGATGAC ACTCCACTCC ATGATTCTGC TAGTAGTGGG 
CACAGAGATA TAGTAAAGCT GTTACTTCGT CACGGTGGAA ATCCATTTCA AGCTAATAAA 
CATGGGGAGC GTCCAGTGGA TGTAGCAGAA ACAGAGGAGT TGGAGTTGCT ACTAAAAAGA 
GAGGTGCCTT TATCTGATGA TGATGAAAGT TACACAGATT CCGAAGAGGC TCAATCTGTA 
AATCCTTCTA GTGTTGATGA AAATATTGAC TCTGAAACAG AGAAAGACTC TCTCATCTGT 
GAAAGTAAAC AGATACTTCC CAGTAAAACA CCTCTTCCAT CTGCCCTTGA TGAGTATGAG 
TTCAAAGATG ATGATGATGA AGAAATTAAT AAGATGATTG ATGATAGGCA TATTCTTAGG 
AAAGAACAAC GAAAAGAAAA TGAACCTGAA GCAGAAAAAA CTCATTTATT TGCAAAACAG 
GAGAAAGCCT TCTATCCTAA ATCATTTAAA AGTAAAAAAC AAAAGCCATC TAGGGTCTTA 
TATTCAAGTA CTGAAAGTTC TGATGAAGAA GCTCTTCAGA ATAAAAAGAT TTCTACTTCA 
TGTTCCGTCA TCCCTGAAAC ATCAAATTCT GATATGCAAA CCAAAAAGGA ATATGTAGTT 
TCAGGTGAAC ACAAACAGAA AGGCAAAGTT AAAAGAAAAT TGAAAAATCA GAATAAAAAT 
AAAGAGAACC AAGAGCTAAA GCAAGAAAAG GAAGGAAAAG AAAATACAAG AATAACAAAC 
TTGACAGTAA ATACTGGACT AGATTGTTCA GAAAAGACCA GAGAGGAGGG GAACTTTAGG 
AAATCTTTTA GCCCAAAAGA TGATACTTCA TTACATTTAT TTCATATTTC CACTGGTAAA 
TCTCCCAAAC ATTCTTGTGG ATTAAGTGAA AAACAGTCAA CACCACTAAA ACAAGAACAT 
ACTAAAACAT GTTTATCACC AGGAAGTTCT GAAATGTCAT TACAGCCTGA TCTTGTTCGG 
TATGATAATA CAGAATCTGA ATTCTTGCCA GAAAGTTCAA GTGTAAAATC TTGTAAGCAT 
AAGGAAAAAA GCAAACATCA GAAAGATTTC CACTTAGAAT TTGGTGAAAA ATCAAATGCC 
AAAATAAAGG ATGAAGATCA TAGTCCAACA TTTGAAAATT CAGATTGCAC ACTGAAAAAA 
ATGGATAAAG AAGGTAAAAC ATTAAAAAAA CATAAATTGA AGCATAAAGA GAGGGAAAAA 
GAAAAGCATA AAAAAGAAAT TGAAGGTGAA AAGGAAAAAT ACAAAACTAA GGATAGTGCC 
AAAGAACTGC AGAGGAGTGT GGAATTTGAT AGAGAATTTT GGAAAGAGAA TTTTTTTAAA 
AGTGATGAAA CTGAAGATCT CTTTTTAAAT ATGGAACATG AATCCTTAAC ATTAGAAAAA 
AAATCAAAAT TGGAAAAAAA CATCAAAGAT GATAAATCAA CCAAGGAAAA GCATGTGTCA 
AAAGAGAGGA ACTTTAAAGA GGAACGAGAC AAGATTAAAA AGGAAAGCGA GAAATCTTTT 
AGGGAGGAAA AAATAAAAGA TCTAAAAGAA GAGAGAGAAA ACATACCCAC AGATAAAGAC 
TCAGAATTTA CTTCTTTGGG TATGAGTGCC ATTGAGGAAT CTATAGGGCT TCATTTAGTG 
GAAAAGGAAA TAGACATTGA AAAACAAGAA AAGCATATAA AGGAAAGTAA AGAAAAACCT 
GAGAAGCGAT CTCAAATTAA AGAAAAGGAC ATTGAGAAGA TGGAAAGAAA AACCTTTGAA 
AAAGAAAAGA AGATAAAACA TGAGCATAAG TCAGAAAAAG ACAAATTAGA TCTTAGTGAA 
TGTGTTGATA AAATAAAAGA AAAGGACAAG CTATATTCGC ATCACACAGA AAAATGCCAT 
AAAGAAGGTG AGAAGAGCAA AAATACTGCT GCTATTAAAA AAACTGACGA CAGAGAGAAA 
AGTAGAGAAA AGATGGATAG GAAACATGAC AAAGAAAAGC CTGAAAAAGA GAGGCATCTA 
GCAGAAAGCA AAGAAAAGCA CTTGATGGAG AAAAAAAATA AACAATCAGA TAATAGTGAA 
TACAGTAAAT CAGAAAAAGG CAAAAATAAA GAAAAAGACA GGGAGCTAGA TAAAAAGGAA 
AAATCTAGAG ATAAAGAAAG TATAAATATA ACTAACTCCA AACACATACA GGAAGAAAAA 
AAATCAAGTA TAGTAGACGG TAATAAAGCA CAACATGAAA AACCCTTATC CCTTAAAGAA 
AAAACAAAAG ATGAACCTTT GAAAACTCCA GATGGAAAAG AAAAAGATAA AAAAGATAAA 
GATATAGATA GATACAAAGA ACGAGACAAA CATAAAGATA AAATTCAAAT AAATAGCTTA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCTAAGT CATCACCAGC ATCAAAAGAT 
ACCCGACCTA AAGAAAAGAG GTTAGTGAAT GATGATTTAA TGCAGACAAG TTTTGAACGA 
ATGCTAAGCC TTAAAGACCT AGAAATAGAA CAGTGGCACA AAAAACATAA GGAAAAAATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAC CGAAACTGTT TAGAACTTAA AATAAAAGAT 
AAAGAAAAAA CAAAGCATAC ACCAACTGAA TCCAAAAATA AAGAACTTAC TAGGTCAAAG 
AGTTCAGAAG TGACTGATGC ATATACCAAG GAGAAACAAC CTAAAGATGC TGTAAGTAAC 
AGATCACAAT CTGTTGACAC CAAAAATGTA ATGACTTTAG GGAAGTCATC TTTTGTTTCA 
GATAATAGCT TAAACAGGTC TCCTAGATCA GAAAATGAAA AGCCGGGTCT CAGCTCCAGA 
TCTGTATCCA TGATTTCTGT TGCTAGTTCA GAAGATTCCT GCCATACTAC AGTGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCTGAC TTTATGTTAG AGAGTTCAGA ATCCCAAATG 
TCCTTTTCCC AGTCACCTTT TTTGTCAATT GCCAAATCTC CTGCTCTTCA TGAAAGGGAA 
TTGGACAGCC TGGCTGACTT GCCGGAGCGG ATTAAACCAC CATATGCAAA CAGACTTTCA 
ACATCCCATC TTAGGTCATC TTCTGTAGAA GATGTTAAAC TAATTATAAG CGAGGGGAGA 
CCTACCATAG AAGTTCGAAG ATGTAGCATG CCTTCTGTCA TTTGTGAACA TACCAAACAA 
TTCCAAACAA TATCAGAAGA GAGCAATCAA GGTAGCTTAT TAACTGTGCC AGGAGATACT 
AGTCCTTCTC CCAAACCTGA GGTATTCTCA AATGTGCCTG AAAGAGACCT TTCAAATGTA 
TCTAACATAC ATTCCAGTTT TGCAACTTCT CCAACTGGAG CTTCAAACAG CAAGTATGTT 
TCAGCTGATA GAAATCTCAT CAAGAATACT GCCCCAGTGA ACACTGTAAT GGACAGTCCA 
GTGCATTTAG AGCCATCTAG TCAGGTTGGT GTGATCCAGA ATAAATCATG GGAGATGCCT 
GTTGATAGAC TAGAGACATT AAGCACCAGA GACTTTATCT GCCCAAATTC TAACATACCT 
GATCAAGAAT CCTCTCTTCA GAGTTTTTGT AATTCTGAAA ATAAGGTATT GAAAGAAAAT 
GCTGATTTTT TATCCCTGCG CCAGACTGAA CTGCCAGGAA ACTCTTGTGC TCAGGATCCG 
GCATCCTTTA TGCCTCCACA GCAGCCTTGC TCTTTCCCCA GCCAATCACT TTCAGATGCT 
GAATCGATTT CTAAACATAT GTCTTTGTCA TATGTTGCTA ATCAAGAGCC AGGTATTTTA 
CAACAAAAAA ATGCAGTTCA GATTATTAGT TCTGCTTTAG ATACTGATAA TGAATCTACA 
AAAGATACAG AAAATACTTT TGTCCTAGGA GATGTTCAAA AAACAGATGC CTTTGTCCCA 
GTGTACTCTG ACAGCACTAT TCAAGAAGCA TCACCAAACT TTGAGAAAGC TTATACTTTA 
CCTGTGTTAC CATCAGAAAA GGACTTTAAT GGAAGTGATG CCTCTACCCA GCTAAATACA 
CATTATGCAT TTAGCAAACT AACTTACAAG TCTTCCAGTG GCCATGAAGT TGAGAATAGC 
ACAACTGATA CTCAGGTCAT TTCACATGAA AAAGAAAACA AACTGGAGAG TTTGGTTTTA 
ACTCATTTGA GTAGGTGTGA TTCTGATTTA TGTGAAATGA ATGCAGGGAT GCCAAAAGGA 
AACCTAAATG AACAAGATCC AAAACATTGT CCTGAAAGTG AAAAGTGTTT GCTTTCCATA 
GAAGATGAGG AATCTCAACA AAGCATTTTA TCAAGTCTGG AAAACCATTC ACAGCAGTCA 
ACTCAACCAG AAATGCATAA ATATGGTCAG TTAGTTAAAG TAGAATTAGA AGAAAATGCC 
GAAGATGATA AAACTGAAAA CCAAATCCCT CAAAGAATGA CTAGAAACAA AGCAAATACA 
ATGGCAAATC AAAGCAAACA GATTCTTGCT AGCTGTACAC TATTATCAGA AAAAGACAGT 
GAATCCTCAT CTCCTAGAGG AAGAATAAGA TTAACTGAAG ATGACGATCC TCAAATTCAC 
CATCCACGGA AAAGGAAAGT GTCACGTGTA CCTCAGCCTG TGCAAGTGAG TCCCTCTTTA 
CTACAAGCAA AAGAGAAAAC TCAGCAATCT CTGGCAGCCA TTGTAGATTC TCTAAAACTA 
GATGAGATTC AGCCATACAG TTCAGAGAGA GCAAATCCAT ATTTTGAATA CTTGCACATA 
AGGAAAAAAA TAGAAGAAAA ACGCAAATTA CTGTGTAGTG TGATTCCTCA AGCACCTCAG 
TACTATGACG AATATGTAAC ATTTAACGGA TCATATCTCC TGGATGGAAA CCCCTTAAGC 
AAGATTTGTA TTCCCACAAT TACACCACCA CCTTCACTGT CAGATCCACT TAAAGAGCTT 
TTTCGACAAC AGGAAGTTGT AAGGATGAAA CTACGTTTGC AACACAGTAT TGAAAGGGAA 
AAACTCATTG TATCCAACGA ACAAGAAGTT TTGCGAGTTC ATTACAGAGC TGCAAGAACA 
TTGGCAAATC AAACACTGCC ATTTAGTGCT TGTACTGTTT TGCTGGATGC CGAAGTATAC 
AATGTACCAT TGGACTCTCA GTCTGATGAC AGTAAAACTT CTGTGAGGGA TCGCTTTAAT 
GCAAGACAAT TCATGTCTTG GTTACAAGAT GTGGATGATA AATTTGACAA ATTAAAGACC 
TGTCTTTTAA TGAGGCAACA ACATGAAGCT GCGGCTTTAA ATGCTGTCCA GAGGTTAGAA 
TGGCAGCTCA AACTCCAGGA ACTTGATCCT GCCACCTATA AATCTATCAG CATTTACGAA 
ATCCAGGAGT TTTATGTTCC CCTTGTTGAT GTTAACGACG ACTTTGAATT GACTCCTATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881151.1
CDS	411..6593
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNNSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025662.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025662.1

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAATTCC ACACCAAATC ATCCATCACA AACAACGCCT 
GCCCAAAAGA AAACTCCCAG TTCTTCATCT CGACAGAAAG ATAAAGTTAA TAAAAGAAAT 
GAACGTGGTG AAACTCCTTT ACACATGGCT GCTATTCGAG GAGATGTGAA ACAAGTTAAA 
GAATTAATAA GTTTAGGGGC AAATGTGAAT GTGAAAGATT TTGCAGGTTG GACACCACTG 
CATGAAGCTT GCAATGTTGG ATATTACGAT GTTGCTAAGA TACTTATAGC AGCTGGAGCA 
GATGTTAACA CACAAGGATT AGATGATGAC ACTCCACTCC ATGATTCTGC TAGTAGTGGG 
CACAGAGATA TAGTAAAGCT GTTACTTCGT CACGGTGGAA ATCCATTTCA AGCTAATAAA 
CATGGGGAGC GTCCAGTGGA TGTAGCAGAA ACAGAGGAGT TGGAGTTGCT ACTAAAAAGA 
GAGGTGCCTT TATCTGATGA TGATGAAAGT TACACAGATT CCGAAGAGGC TCAATCTGTA 
AATCCTTCTA GTGTTGATGA AAATATTGAC TCTGAAACAG AGAAAGACTC TCTCATCTGT 
GAAAGTAAAC AGATACTTCC CAGTAAAACA CCTCTTCCAT CTGCCCTTGA TGAGTATGAG 
TTCAAAGATG ATGATGATGA AGAAATTAAT AAGATGATTG ATGATAGGCA TATTCTTAGG 
AAAGAACAAC GAAAAGAAAA TGAACCTGAA GCAGAAAAAA CTCATTTATT TGCAAAACAG 
GAGAAAGCCT TCTATCCTAA ATCATTTAAA AGTAAAAAAC AAAAGCCATC TAGGGTCTTA 
TATTCAAGTA CTGAAAGTTC TGATGAAGAA GCTCTTCAGA ATAAAAAGAT TTCTACTTCA 
TGTTCCGTCA TCCCTGAAAC ATCAAATTCT GATATGCAAA CCAAAAAGGA ATATGTAGTT 
TCAGGTGAAC ACAAACAGAA AGGCAAAGTT AAAAGAAAAT TGAAAAATCA GAATAAAAAT 
AAAGAGAACC AAGAGCTAAA GCAAGAAAAG GAAGGAAAAG AAAATACAAG AATAACAAAC 
TTGACAGTAA ATACTGGACT AGATTGTTCA GAAAAGACCA GAGAGGAGGG GAACTTTAGG 
AAATCTTTTA GCCCAAAAGA TGATACTTCA TTACATTTAT TTCATATTTC CACTGGTAAA 
TCTCCCAAAC ATTCTTGTGG ATTAAGTGAA AAACAGTCAA CACCACTAAA ACAAGAACAT 
ACTAAAACAT GTTTATCACC AGGAAGTTCT GAAATGTCAT TACAGCCTGA TCTTGTTCGG 
TATGATAATA CAGAATCTGA ATTCTTGCCA GAAAGTTCAA GTGTAAAATC TTGTAAGCAT 
AAGGAAAAAA GCAAACATCA GAAAGATTTC CACTTAGAAT TTGGTGAAAA ATCAAATGCC 
AAAATAAAGG ATGAAGATCA TAGTCCAACA TTTGAAAATT CAGATTGCAC ACTGAAAAAA 
ATGGATAAAG AAGGTAAAAC ATTAAAAAAA CATAAATTGA AGCATAAAGA GAGGGAAAAA 
GAAAAGCATA AAAAAGAAAT TGAAGGTGAA AAGGAAAAAT ACAAAACTAA GGATAGTGCC 
AAAGAACTGC AGAGGAGTGT GGAATTTGAT AGAGAATTTT GGAAAGAGAA TTTTTTTAAA 
AGTGATGAAA CTGAAGATCT CTTTTTAAAT ATGGAACATG AATCCTTAAC ATTAGAAAAA 
AAATCAAAAT TGGAAAAAAA CATCAAAGAT GATAAATCAA CCAAGGAAAA GCATGTGTCA 
AAAGAGAGGA ACTTTAAAGA GGAACGAGAC AAGATTAAAA AGGAAAGCGA GAAATCTTTT 
AGGGAGGAAA AAATAAAAGA TCTAAAAGAA GAGAGAGAAA ACATACCCAC AGATAAAGAC 
TCAGAATTTA CTTCTTTGGG TATGAGTGCC ATTGAGGAAT CTATAGGGCT TCATTTAGTG 
GAAAAGGAAA TAGACATTGA AAAACAAGAA AAGCATATAA AGGAAAGTAA AGAAAAACCT 
GAGAAGCGAT CTCAAATTAA AGAAAAGGAC ATTGAGAAGA TGGAAAGAAA AACCTTTGAA 
AAAGAAAAGA AGATAAAACA TGAGCATAAG TCAGAAAAAG ACAAATTAGA TCTTAGTGAA 
TGTGTTGATA AAATAAAAGA AAAGGACAAG CTATATTCGC ATCACACAGA AAAATGCCAT 
AAAGAAGGTG AGAAGAGCAA AAATACTGCT GCTATTAAAA AAACTGACGA CAGAGAGAAA 
AGTAGAGAAA AGATGGATAG GAAACATGAC AAAGAAAAGC CTGAAAAAGA GAGGCATCTA 
GCAGAAAGCA AAGAAAAGCA CTTGATGGAG AAAAAAAATA AACAATCAGA TAATAGTGAA 
TACAGTAAAT CAGAAAAAGG CAAAAATAAA GAAAAAGACA GGGAGCTAGA TAAAAAGGAA 
AAATCTAGAG ATAAAGAAAG TATAAATATA ACTAACTCCA AACACATACA GGAAGAAAAA 
AAATCAAGTA TAGTAGACGG TAATAAAGCA CAACATGAAA AACCCTTATC CCTTAAAGAA 
AAAACAAAAG ATGAACCTTT GAAAACTCCA GATGGAAAAG AAAAAGATAA AAAAGATAAA 
GATATAGATA GATACAAAGA ACGAGACAAA CATAAAGATA AAATTCAAAT AAATAGCTTA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCTAAGT CATCACCAGC ATCAAAAGAT 
ACCCGACCTA AAGAAAAGAG GTTAGTGAAT GATGATTTAA TGCAGACAAG TTTTGAACGA 
ATGCTAAGCC TTAAAGACCT AGAAATAGAA CAGTGGCACA AAAAACATAA GGAAAAAATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAC CGAAACTGTT TAGAACTTAA AATAAAAGAT 
AAAGAAAAAA CAAAGCATAC ACCAACTGAA TCCAAAAATA AAGAACTTAC TAGGTCAAAG 
AGTTCAGAAG TGACTGATGC ATATACCAAG GAGAAACAAC CTAAAGATGC TGTAAGTAAC 
AGATCACAAT CTGTTGACAC CAAAAATGTA ATGACTTTAG GGAAGTCATC TTTTGTTTCA 
GATAATAGCT TAAACAGGTC TCCTAGATCA GAAAATGAAA AGCCGGGTCT CAGCTCCAGA 
TCTGTATCCA TGATTTCTGT TGCTAGTTCA GAAGATTCCT GCCATACTAC AGTGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCTGAC TTTATGTTAG AGAGTTCAGA ATCCCAAATG 
TCCTTTTCCC AGTCACCTTT TTTGTCAATT GCCAAATCTC CTGCTCTTCA TGAAAGGGAA 
TTGGACAGCC TGGCTGACTT GCCGGAGCGG ATTAAACCAC CATATGCAAA CAGACTTTCA 
ACATCCCATC TTAGGTCATC TTCTGTAGAA GATGTTAAAC TAATTATAAG CGAGGGGAGA 
CCTACCATAG AAGTTCGAAG ATGTAGCATG CCTTCTGTCA TTTGTGAACA TACCAAACAA 
TTCCAAACAA TATCAGAAGA GAGCAATCAA GGTAGCTTAT TAACTGTGCC AGGAGATACT 
AGTCCTTCTC CCAAACCTGA GGTATTCTCA AATGTGCCTG AAAGAGACCT TTCAAATGTA 
TCTAACATAC ATTCCAGTTT TGCAACTTCT CCAACTGGAG CTTCAAACAG CAAGTATGTT 
TCAGCTGATA GAAATCTCAT CAAGAATACT GCCCCAGTGA ACACTGTAAT GGACAGTCCA 
GTGCATTTAG AGCCATCTAG TCAGGTTGGT GTGATCCAGA ATAAATCATG GGAGATGCCT 
GTTGATAGAC TAGAGACATT AAGCACCAGA GACTTTATCT GCCCAAATTC TAACATACCT 
GATCAAGAAT CCTCTCTTCA GAGTTTTTGT AATTCTGAAA ATAAGGTATT GAAAGAAAAT 
GCTGATTTTT TATCCCTGCG CCAGACTGAA CTGCCAGGAA ACTCTTGTGC TCAGGATCCG 
GCATCCTTTA TGCCTCCACA GCAGCCTTGC TCTTTCCCCA GCCAATCACT TTCAGATGCT 
GAATCGATTT CTAAACATAT GTCTTTGTCA TATGTTGCTA ATCAAGAGCC AGGTATTTTA 
CAACAAAAAA ATGCAGTTCA GATTATTAGT TCTGCTTTAG ATACTGATAA TGAATCTACA 
AAAGATACAG AAAATACTTT TGTCCTAGGA GATGTTCAAA AAACAGATGC CTTTGTCCCA 
GTGTACTCTG ACAGCACTAT TCAAGAAGCA TCACCAAACT TTGAGAAAGC TTATACTTTA 
CCTGTGTTAC CATCAGAAAA GGACTTTAAT GGAAGTGATG CCTCTACCCA GCTAAATACA 
CATTATGCAT TTAGCAAACT AACTTACAAG TCTTCCAGTG GCCATGAAGT TGAGAATAGC 
ACAACTGATA CTCAGGTCAT TTCACATGAA AAAGAAAACA AACTGGAGAG TTTGGTTTTA 
ACTCATTTGA GTAGGTGTGA TTCTGATTTA TGTGAAATGA ATGCAGGGAT GCCAAAAGGA 
AACCTAAATG AACAAGATCC AAAACATTGT CCTGAAAGTG AAAAGTGTTT GCTTTCCATA 
GAAGATGAGG AATCTCAACA AAGCATTTTA TCAAGTCTGG AAAACCATTC ACAGCAGTCA 
ACTCAACCAG AAATGCATAA ATATGGTCAG TTAGTTAAAG TAGAATTAGA AGAAAATGCC 
GAAGATGATA AAACTGAAAA CCAAATCCCT CAAAGAATGA CTAGAAACAA AGCAAATACA 
ATGGCAAATC AAAGCAAACA GATTCTTGCT AGCTGTACAC TATTATCAGA AAAAGACAGT 
GAATCCTCAT CTCCTAGAGG AAGAATAAGA TTAACTGAAG ATGACGATCC TCAAATTCAC 
CATCCACGGA AAAGGAAAGT GTCACGTGTA CCTCAGCCTG TGCAAGTGAG TCCCTCTTTA 
CTACAAGCAA AAGAGAAAAC TCAGCAATCT CTGGCAGCCA TTGTAGATTC TCTAAAACTA 
GATGAGATTC AGCCATACAG TTCAGAGAGA GCAAATCCAT ATTTTGAATA CTTGCACATA 
AGGAAAAAAA TAGAAGAAAA ACGCAAATTA CTGTGTAGTG TGATTCCTCA AGCACCTCAG 
TACTATGACG AATATGTAAC ATTTAACGGA TCATATCTCC TGGATGGAAA CCCCTTAAGC 
AAGATTTGTA TTCCCACAAT TACACCACCA CCTTCACTGT CAGATCCACT TAAAGAGCTT 
TTTCGACAAC AGGAAGTTGT AAGGATGAAA CTACGTTTGC AACACAGTAT TGAAAGGGAA 
AAACTCATTG TATCCAACGA ACAAGAAGTT TTGCGAGTTC ATTACAGAGC TGCAAGAACA 
TTGGCAAATC AAACACTGCC ATTTAGTGCT TGTACTGTTT TGCTGGATGC CGAAGTATAC 
AATGTACCAT TGGACTCTCA GTCTGATGAC AGTAAAACTT CTGTGAGGGA TCGCTTTAAT 
GCAAGACAAT TCATGTCTTG GTTACAAGAT GTGGATGATA AATTTGACAA ATTAAAGACC 
TGTCTTTTAA TGAGGCAACA ACATGAAGCT GCGGCTTTAA ATGCTGTCCA GAGGTTAGAA 
TGGCAGCTCA AACTCCAGGA ACTTGATCCT GCCACCTATA AATCTATCAG CATTTACGAA 
ATCCAGGAGT TTTATGTTCC CCTTGTTGAT GTTAACGACG ACTTTGAATT GACTCCTATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025661.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881150.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X4, mRNA.

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CloneID	OHu50996
Clone ID Related Accession (Same CDS sequence)	XM_005258095.3 , XM_017025662.1 , XM_017025661.1 , XM_017025661.2 , XM_017025662.2 , XM_005258095.2
Accession Version	XM_017025661.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAATTCC ACACCAAATC ATCCATCACA AACAACGCCT 
GCCCAAAAGA AAACTCCCAG TTCTTCATCT CGACAGAAAG ATAAAGTTAA TAAAAGAAAT 
GAACGTGGTG AAACTCCTTT ACACATGGCT GCTATTCGAG GAGATGTGAA ACAAGTTAAA 
GAATTAATAA GTTTAGGGGC AAATGTGAAT GTGAAAGATT TTGCAGGTTG GACACCACTG 
CATGAAGCTT GCAATGTTGG ATATTACGAT GTTGCTAAGA TACTTATAGC AGCTGGAGCA 
GATGTTAACA CACAAGGATT AGATGATGAC ACTCCACTCC ATGATTCTGC TAGTAGTGGG 
CACAGAGATA TAGTAAAGCT GTTACTTCGT CACGGTGGAA ATCCATTTCA AGCTAATAAA 
CATGGGGAGC GTCCAGTGGA TGTAGCAGAA ACAGAGGAGT TGGAGTTGCT ACTAAAAAGA 
GAGGTGCCTT TATCTGATGA TGATGAAAGT TACACAGATT CCGAAGAGGC TCAATCTGTA 
AATCCTTCTA GTGTTGATGA AAATATTGAC TCTGAAACAG AGAAAGACTC TCTCATCTGT 
GAAAGTAAAC AGATACTTCC CAGTAAAACA CCTCTTCCAT CTGCCCTTGA TGAGTATGAG 
TTCAAAGATG ATGATGATGA AGAAATTAAT AAGATGATTG ATGATAGGCA TATTCTTAGG 
AAAGAACAAC GAAAAGAAAA TGAACCTGAA GCAGAAAAAA CTCATTTATT TGCAAAACAG 
GAGAAAGCCT TCTATCCTAA ATCATTTAAA AGTAAAAAAC AAAAGCCATC TAGGGTCTTA 
TATTCAAGTA CTGAAAGTTC TGATGAAGAA GCTCTTCAGA ATAAAAAGAT TTCTACTTCA 
TGTTCCGTCA TCCCTGAAAC ATCAAATTCT GATATGCAAA CCAAAAAGGA ATATGTAGTT 
TCAGGTGAAC ACAAACAGAA AGGCAAAGTT AAAAGAAAAT TGAAAAATCA GAATAAAAAT 
AAAGAGAACC AAGAGCTAAA GCAAGAAAAG GAAGGAAAAG AAAATACAAG AATAACAAAC 
TTGACAGTAA ATACTGGACT AGATTGTTCA GAAAAGACCA GAGAGGAGGG GAACTTTAGG 
AAATCTTTTA GCCCAAAAGA TGATACTTCA TTACATTTAT TTCATATTTC CACTGGTAAA 
TCTCCCAAAC ATTCTTGTGG ATTAAGTGAA AAACAGTCAA CACCACTAAA ACAAGAACAT 
ACTAAAACAT GTTTATCACC AGGAAGTTCT GAAATGTCAT TACAGCCTGA TCTTGTTCGG 
TATGATAATA CAGAATCTGA ATTCTTGCCA GAAAGTTCAA GTGTAAAATC TTGTAAGCAT 
AAGGAAAAAA GCAAACATCA GAAAGATTTC CACTTAGAAT TTGGTGAAAA ATCAAATGCC 
AAAATAAAGG ATGAAGATCA TAGTCCAACA TTTGAAAATT CAGATTGCAC ACTGAAAAAA 
ATGGATAAAG AAGGTAAAAC ATTAAAAAAA CATAAATTGA AGCATAAAGA GAGGGAAAAA 
GAAAAGCATA AAAAAGAAAT TGAAGGTGAA AAGGAAAAAT ACAAAACTAA GGATAGTGCC 
AAAGAACTGC AGAGGAGTGT GGAATTTGAT AGAGAATTTT GGAAAGAGAA TTTTTTTAAA 
AGTGATGAAA CTGAAGATCT CTTTTTAAAT ATGGAACATG AATCCTTAAC ATTAGAAAAA 
AAATCAAAAT TGGAAAAAAA CATCAAAGAT GATAAATCAA CCAAGGAAAA GCATGTGTCA 
AAAGAGAGGA ACTTTAAAGA GGAACGAGAC AAGATTAAAA AGGAAAGCGA GAAATCTTTT 
AGGGAGGAAA AAATAAAAGA TCTAAAAGAA GAGAGAGAAA ACATACCCAC AGATAAAGAC 
TCAGAATTTA CTTCTTTGGG TATGAGTGCC ATTGAGGAAT CTATAGGGCT TCATTTAGTG 
GAAAAGGAAA TAGACATTGA AAAACAAGAA AAGCATATAA AGGAAAGTAA AGAAAAACCT 
GAGAAGCGAT CTCAAATTAA AGAAAAGGAC ATTGAGAAGA TGGAAAGAAA AACCTTTGAA 
AAAGAAAAGA AGATAAAACA TGAGCATAAG TCAGAAAAAG ACAAATTAGA TCTTAGTGAA 
TGTGTTGATA AAATAAAAGA AAAGGACAAG CTATATTCGC ATCACACAGA AAAATGCCAT 
AAAGAAGGTG AGAAGAGCAA AAATACTGCT GCTATTAAAA AAACTGACGA CAGAGAGAAA 
AGTAGAGAAA AGATGGATAG GAAACATGAC AAAGAAAAGC CTGAAAAAGA GAGGCATCTA 
GCAGAAAGCA AAGAAAAGCA CTTGATGGAG AAAAAAAATA AACAATCAGA TAATAGTGAA 
TACAGTAAAT CAGAAAAAGG CAAAAATAAA GAAAAAGACA GGGAGCTAGA TAAAAAGGAA 
AAATCTAGAG ATAAAGAAAG TATAAATATA ACTAACTCCA AACACATACA GGAAGAAAAA 
AAATCAAGTA TAGTAGACGG TAATAAAGCA CAACATGAAA AACCCTTATC CCTTAAAGAA 
AAAACAAAAG ATGAACCTTT GAAAACTCCA GATGGAAAAG AAAAAGATAA AAAAGATAAA 
GATATAGATA GATACAAAGA ACGAGACAAA CATAAAGATA AAATTCAAAT AAATAGCTTA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCTAAGT CATCACCAGC ATCAAAAGAT 
ACCCGACCTA AAGAAAAGAG GTTAGTGAAT GATGATTTAA TGCAGACAAG TTTTGAACGA 
ATGCTAAGCC TTAAAGACCT AGAAATAGAA CAGTGGCACA AAAAACATAA GGAAAAAATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAC CGAAACTGTT TAGAACTTAA AATAAAAGAT 
AAAGAAAAAA CAAAGCATAC ACCAACTGAA TCCAAAAATA AAGAACTTAC TAGGTCAAAG 
AGTTCAGAAG TGACTGATGC ATATACCAAG GAGAAACAAC CTAAAGATGC TGTAAGTAAC 
AGATCACAAT CTGTTGACAC CAAAAATGTA ATGACTTTAG GGAAGTCATC TTTTGTTTCA 
GATAATAGCT TAAACAGGTC TCCTAGATCA GAAAATGAAA AGCCGGGTCT CAGCTCCAGA 
TCTGTATCCA TGATTTCTGT TGCTAGTTCA GAAGATTCCT GCCATACTAC AGTGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCTGAC TTTATGTTAG AGAGTTCAGA ATCCCAAATG 
TCCTTTTCCC AGTCACCTTT TTTGTCAATT GCCAAATCTC CTGCTCTTCA TGAAAGGGAA 
TTGGACAGCC TGGCTGACTT GCCGGAGCGG ATTAAACCAC CATATGCAAA CAGACTTTCA 
ACATCCCATC TTAGGTCATC TTCTGTAGAA GATGTTAAAC TAATTATAAG CGAGGGGAGA 
CCTACCATAG AAGTTCGAAG ATGTAGCATG CCTTCTGTCA TTTGTGAACA TACCAAACAA 
TTCCAAACAA TATCAGAAGA GAGCAATCAA GGTAGCTTAT TAACTGTGCC AGGAGATACT 
AGTCCTTCTC CCAAACCTGA GGTATTCTCA AATGTGCCTG AAAGAGACCT TTCAAATGTA 
TCTAACATAC ATTCCAGTTT TGCAACTTCT CCAACTGGAG CTTCAAACAG CAAGTATGTT 
TCAGCTGATA GAAATCTCAT CAAGAATACT GCCCCAGTGA ACACTGTAAT GGACAGTCCA 
GTGCATTTAG AGCCATCTAG TCAGGTTGGT GTGATCCAGA ATAAATCATG GGAGATGCCT 
GTTGATAGAC TAGAGACATT AAGCACCAGA GACTTTATCT GCCCAAATTC TAACATACCT 
GATCAAGAAT CCTCTCTTCA GAGTTTTTGT AATTCTGAAA ATAAGGTATT GAAAGAAAAT 
GCTGATTTTT TATCCCTGCG CCAGACTGAA CTGCCAGGAA ACTCTTGTGC TCAGGATCCG 
GCATCCTTTA TGCCTCCACA GCAGCCTTGC TCTTTCCCCA GCCAATCACT TTCAGATGCT 
GAATCGATTT CTAAACATAT GTCTTTGTCA TATGTTGCTA ATCAAGAGCC AGGTATTTTA 
CAACAAAAAA ATGCAGTTCA GATTATTAGT TCTGCTTTAG ATACTGATAA TGAATCTACA 
AAAGATACAG AAAATACTTT TGTCCTAGGA GATGTTCAAA AAACAGATGC CTTTGTCCCA 
GTGTACTCTG ACAGCACTAT TCAAGAAGCA TCACCAAACT TTGAGAAAGC TTATACTTTA 
CCTGTGTTAC CATCAGAAAA GGACTTTAAT GGAAGTGATG CCTCTACCCA GCTAAATACA 
CATTATGCAT TTAGCAAACT AACTTACAAG TCTTCCAGTG GCCATGAAGT TGAGAATAGC 
ACAACTGATA CTCAGGTCAT TTCACATGAA AAAGAAAACA AACTGGAGAG TTTGGTTTTA 
ACTCATTTGA GTAGGTGTGA TTCTGATTTA TGTGAAATGA ATGCAGGGAT GCCAAAAGGA 
AACCTAAATG AACAAGATCC AAAACATTGT CCTGAAAGTG AAAAGTGTTT GCTTTCCATA 
GAAGATGAGG AATCTCAACA AAGCATTTTA TCAAGTCTGG AAAACCATTC ACAGCAGTCA 
ACTCAACCAG AAATGCATAA ATATGGTCAG TTAGTTAAAG TAGAATTAGA AGAAAATGCC 
GAAGATGATA AAACTGAAAA CCAAATCCCT CAAAGAATGA CTAGAAACAA AGCAAATACA 
ATGGCAAATC AAAGCAAACA GATTCTTGCT AGCTGTACAC TATTATCAGA AAAAGACAGT 
GAATCCTCAT CTCCTAGAGG AAGAATAAGA TTAACTGAAG ATGACGATCC TCAAATTCAC 
CATCCACGGA AAAGGAAAGT GTCACGTGTA CCTCAGCCTG TGCAAGTGAG TCCCTCTTTA 
CTACAAGCAA AAGAGAAAAC TCAGCAATCT CTGGCAGCCA TTGTAGATTC TCTAAAACTA 
GATGAGATTC AGCCATACAG TTCAGAGAGA GCAAATCCAT ATTTTGAATA CTTGCACATA 
AGGAAAAAAA TAGAAGAAAA ACGCAAATTA CTGTGTAGTG TGATTCCTCA AGCACCTCAG 
TACTATGACG AATATGTAAC ATTTAACGGA TCATATCTCC TGGATGGAAA CCCCTTAAGC 
AAGATTTGTA TTCCCACAAT TACACCACCA CCTTCACTGT CAGATCCACT TAAAGAGCTT 
TTTCGACAAC AGGAAGTTGT AAGGATGAAA CTACGTTTGC AACACAGTAT TGAAAGGGAA 
AAACTCATTG TATCCAACGA ACAAGAAGTT TTGCGAGTTC ATTACAGAGC TGCAAGAACA 
TTGGCAAATC AAACACTGCC ATTTAGTGCT TGTACTGTTT TGCTGGATGC CGAAGTATAC 
AATGTACCAT TGGACTCTCA GTCTGATGAC AGTAAAACTT CTGTGAGGGA TCGCTTTAAT 
GCAAGACAAT TCATGTCTTG GTTACAAGAT GTGGATGATA AATTTGACAA ATTAAAGACC 
TGTCTTTTAA TGAGGCAACA ACATGAAGCT GCGGCTTTAA ATGCTGTCCA GAGGTTAGAA 
TGGCAGCTCA AACTCCAGGA ACTTGATCCT GCCACCTATA AATCTATCAG CATTTACGAA 
ATCCAGGAGT TTTATGTTCC CCTTGTTGAT GTTAACGACG ACTTTGAATT GACTCCTATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881150.1
CDS	366..6548
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNNSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025661.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025661.1

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAATTCC ACACCAAATC ATCCATCACA AACAACGCCT 
GCCCAAAAGA AAACTCCCAG TTCTTCATCT CGACAGAAAG ATAAAGTTAA TAAAAGAAAT 
GAACGTGGTG AAACTCCTTT ACACATGGCT GCTATTCGAG GAGATGTGAA ACAAGTTAAA 
GAATTAATAA GTTTAGGGGC AAATGTGAAT GTGAAAGATT TTGCAGGTTG GACACCACTG 
CATGAAGCTT GCAATGTTGG ATATTACGAT GTTGCTAAGA TACTTATAGC AGCTGGAGCA 
GATGTTAACA CACAAGGATT AGATGATGAC ACTCCACTCC ATGATTCTGC TAGTAGTGGG 
CACAGAGATA TAGTAAAGCT GTTACTTCGT CACGGTGGAA ATCCATTTCA AGCTAATAAA 
CATGGGGAGC GTCCAGTGGA TGTAGCAGAA ACAGAGGAGT TGGAGTTGCT ACTAAAAAGA 
GAGGTGCCTT TATCTGATGA TGATGAAAGT TACACAGATT CCGAAGAGGC TCAATCTGTA 
AATCCTTCTA GTGTTGATGA AAATATTGAC TCTGAAACAG AGAAAGACTC TCTCATCTGT 
GAAAGTAAAC AGATACTTCC CAGTAAAACA CCTCTTCCAT CTGCCCTTGA TGAGTATGAG 
TTCAAAGATG ATGATGATGA AGAAATTAAT AAGATGATTG ATGATAGGCA TATTCTTAGG 
AAAGAACAAC GAAAAGAAAA TGAACCTGAA GCAGAAAAAA CTCATTTATT TGCAAAACAG 
GAGAAAGCCT TCTATCCTAA ATCATTTAAA AGTAAAAAAC AAAAGCCATC TAGGGTCTTA 
TATTCAAGTA CTGAAAGTTC TGATGAAGAA GCTCTTCAGA ATAAAAAGAT TTCTACTTCA 
TGTTCCGTCA TCCCTGAAAC ATCAAATTCT GATATGCAAA CCAAAAAGGA ATATGTAGTT 
TCAGGTGAAC ACAAACAGAA AGGCAAAGTT AAAAGAAAAT TGAAAAATCA GAATAAAAAT 
AAAGAGAACC AAGAGCTAAA GCAAGAAAAG GAAGGAAAAG AAAATACAAG AATAACAAAC 
TTGACAGTAA ATACTGGACT AGATTGTTCA GAAAAGACCA GAGAGGAGGG GAACTTTAGG 
AAATCTTTTA GCCCAAAAGA TGATACTTCA TTACATTTAT TTCATATTTC CACTGGTAAA 
TCTCCCAAAC ATTCTTGTGG ATTAAGTGAA AAACAGTCAA CACCACTAAA ACAAGAACAT 
ACTAAAACAT GTTTATCACC AGGAAGTTCT GAAATGTCAT TACAGCCTGA TCTTGTTCGG 
TATGATAATA CAGAATCTGA ATTCTTGCCA GAAAGTTCAA GTGTAAAATC TTGTAAGCAT 
AAGGAAAAAA GCAAACATCA GAAAGATTTC CACTTAGAAT TTGGTGAAAA ATCAAATGCC 
AAAATAAAGG ATGAAGATCA TAGTCCAACA TTTGAAAATT CAGATTGCAC ACTGAAAAAA 
ATGGATAAAG AAGGTAAAAC ATTAAAAAAA CATAAATTGA AGCATAAAGA GAGGGAAAAA 
GAAAAGCATA AAAAAGAAAT TGAAGGTGAA AAGGAAAAAT ACAAAACTAA GGATAGTGCC 
AAAGAACTGC AGAGGAGTGT GGAATTTGAT AGAGAATTTT GGAAAGAGAA TTTTTTTAAA 
AGTGATGAAA CTGAAGATCT CTTTTTAAAT ATGGAACATG AATCCTTAAC ATTAGAAAAA 
AAATCAAAAT TGGAAAAAAA CATCAAAGAT GATAAATCAA CCAAGGAAAA GCATGTGTCA 
AAAGAGAGGA ACTTTAAAGA GGAACGAGAC AAGATTAAAA AGGAAAGCGA GAAATCTTTT 
AGGGAGGAAA AAATAAAAGA TCTAAAAGAA GAGAGAGAAA ACATACCCAC AGATAAAGAC 
TCAGAATTTA CTTCTTTGGG TATGAGTGCC ATTGAGGAAT CTATAGGGCT TCATTTAGTG 
GAAAAGGAAA TAGACATTGA AAAACAAGAA AAGCATATAA AGGAAAGTAA AGAAAAACCT 
GAGAAGCGAT CTCAAATTAA AGAAAAGGAC ATTGAGAAGA TGGAAAGAAA AACCTTTGAA 
AAAGAAAAGA AGATAAAACA TGAGCATAAG TCAGAAAAAG ACAAATTAGA TCTTAGTGAA 
TGTGTTGATA AAATAAAAGA AAAGGACAAG CTATATTCGC ATCACACAGA AAAATGCCAT 
AAAGAAGGTG AGAAGAGCAA AAATACTGCT GCTATTAAAA AAACTGACGA CAGAGAGAAA 
AGTAGAGAAA AGATGGATAG GAAACATGAC AAAGAAAAGC CTGAAAAAGA GAGGCATCTA 
GCAGAAAGCA AAGAAAAGCA CTTGATGGAG AAAAAAAATA AACAATCAGA TAATAGTGAA 
TACAGTAAAT CAGAAAAAGG CAAAAATAAA GAAAAAGACA GGGAGCTAGA TAAAAAGGAA 
AAATCTAGAG ATAAAGAAAG TATAAATATA ACTAACTCCA AACACATACA GGAAGAAAAA 
AAATCAAGTA TAGTAGACGG TAATAAAGCA CAACATGAAA AACCCTTATC CCTTAAAGAA 
AAAACAAAAG ATGAACCTTT GAAAACTCCA GATGGAAAAG AAAAAGATAA AAAAGATAAA 
GATATAGATA GATACAAAGA ACGAGACAAA CATAAAGATA AAATTCAAAT AAATAGCTTA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCTAAGT CATCACCAGC ATCAAAAGAT 
ACCCGACCTA AAGAAAAGAG GTTAGTGAAT GATGATTTAA TGCAGACAAG TTTTGAACGA 
ATGCTAAGCC TTAAAGACCT AGAAATAGAA CAGTGGCACA AAAAACATAA GGAAAAAATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAC CGAAACTGTT TAGAACTTAA AATAAAAGAT 
AAAGAAAAAA CAAAGCATAC ACCAACTGAA TCCAAAAATA AAGAACTTAC TAGGTCAAAG 
AGTTCAGAAG TGACTGATGC ATATACCAAG GAGAAACAAC CTAAAGATGC TGTAAGTAAC 
AGATCACAAT CTGTTGACAC CAAAAATGTA ATGACTTTAG GGAAGTCATC TTTTGTTTCA 
GATAATAGCT TAAACAGGTC TCCTAGATCA GAAAATGAAA AGCCGGGTCT CAGCTCCAGA 
TCTGTATCCA TGATTTCTGT TGCTAGTTCA GAAGATTCCT GCCATACTAC AGTGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCTGAC TTTATGTTAG AGAGTTCAGA ATCCCAAATG 
TCCTTTTCCC AGTCACCTTT TTTGTCAATT GCCAAATCTC CTGCTCTTCA TGAAAGGGAA 
TTGGACAGCC TGGCTGACTT GCCGGAGCGG ATTAAACCAC CATATGCAAA CAGACTTTCA 
ACATCCCATC TTAGGTCATC TTCTGTAGAA GATGTTAAAC TAATTATAAG CGAGGGGAGA 
CCTACCATAG AAGTTCGAAG ATGTAGCATG CCTTCTGTCA TTTGTGAACA TACCAAACAA 
TTCCAAACAA TATCAGAAGA GAGCAATCAA GGTAGCTTAT TAACTGTGCC AGGAGATACT 
AGTCCTTCTC CCAAACCTGA GGTATTCTCA AATGTGCCTG AAAGAGACCT TTCAAATGTA 
TCTAACATAC ATTCCAGTTT TGCAACTTCT CCAACTGGAG CTTCAAACAG CAAGTATGTT 
TCAGCTGATA GAAATCTCAT CAAGAATACT GCCCCAGTGA ACACTGTAAT GGACAGTCCA 
GTGCATTTAG AGCCATCTAG TCAGGTTGGT GTGATCCAGA ATAAATCATG GGAGATGCCT 
GTTGATAGAC TAGAGACATT AAGCACCAGA GACTTTATCT GCCCAAATTC TAACATACCT 
GATCAAGAAT CCTCTCTTCA GAGTTTTTGT AATTCTGAAA ATAAGGTATT GAAAGAAAAT 
GCTGATTTTT TATCCCTGCG CCAGACTGAA CTGCCAGGAA ACTCTTGTGC TCAGGATCCG 
GCATCCTTTA TGCCTCCACA GCAGCCTTGC TCTTTCCCCA GCCAATCACT TTCAGATGCT 
GAATCGATTT CTAAACATAT GTCTTTGTCA TATGTTGCTA ATCAAGAGCC AGGTATTTTA 
CAACAAAAAA ATGCAGTTCA GATTATTAGT TCTGCTTTAG ATACTGATAA TGAATCTACA 
AAAGATACAG AAAATACTTT TGTCCTAGGA GATGTTCAAA AAACAGATGC CTTTGTCCCA 
GTGTACTCTG ACAGCACTAT TCAAGAAGCA TCACCAAACT TTGAGAAAGC TTATACTTTA 
CCTGTGTTAC CATCAGAAAA GGACTTTAAT GGAAGTGATG CCTCTACCCA GCTAAATACA 
CATTATGCAT TTAGCAAACT AACTTACAAG TCTTCCAGTG GCCATGAAGT TGAGAATAGC 
ACAACTGATA CTCAGGTCAT TTCACATGAA AAAGAAAACA AACTGGAGAG TTTGGTTTTA 
ACTCATTTGA GTAGGTGTGA TTCTGATTTA TGTGAAATGA ATGCAGGGAT GCCAAAAGGA 
AACCTAAATG AACAAGATCC AAAACATTGT CCTGAAAGTG AAAAGTGTTT GCTTTCCATA 
GAAGATGAGG AATCTCAACA AAGCATTTTA TCAAGTCTGG AAAACCATTC ACAGCAGTCA 
ACTCAACCAG AAATGCATAA ATATGGTCAG TTAGTTAAAG TAGAATTAGA AGAAAATGCC 
GAAGATGATA AAACTGAAAA CCAAATCCCT CAAAGAATGA CTAGAAACAA AGCAAATACA 
ATGGCAAATC AAAGCAAACA GATTCTTGCT AGCTGTACAC TATTATCAGA AAAAGACAGT 
GAATCCTCAT CTCCTAGAGG AAGAATAAGA TTAACTGAAG ATGACGATCC TCAAATTCAC 
CATCCACGGA AAAGGAAAGT GTCACGTGTA CCTCAGCCTG TGCAAGTGAG TCCCTCTTTA 
CTACAAGCAA AAGAGAAAAC TCAGCAATCT CTGGCAGCCA TTGTAGATTC TCTAAAACTA 
GATGAGATTC AGCCATACAG TTCAGAGAGA GCAAATCCAT ATTTTGAATA CTTGCACATA 
AGGAAAAAAA TAGAAGAAAA ACGCAAATTA CTGTGTAGTG TGATTCCTCA AGCACCTCAG 
TACTATGACG AATATGTAAC ATTTAACGGA TCATATCTCC TGGATGGAAA CCCCTTAAGC 
AAGATTTGTA TTCCCACAAT TACACCACCA CCTTCACTGT CAGATCCACT TAAAGAGCTT 
TTTCGACAAC AGGAAGTTGT AAGGATGAAA CTACGTTTGC AACACAGTAT TGAAAGGGAA 
AAACTCATTG TATCCAACGA ACAAGAAGTT TTGCGAGTTC ATTACAGAGC TGCAAGAACA 
TTGGCAAATC AAACACTGCC ATTTAGTGCT TGTACTGTTT TGCTGGATGC CGAAGTATAC 
AATGTACCAT TGGACTCTCA GTCTGATGAC AGTAAAACTT CTGTGAGGGA TCGCTTTAAT 
GCAAGACAAT TCATGTCTTG GTTACAAGAT GTGGATGATA AATTTGACAA ATTAAAGACC 
TGTCTTTTAA TGAGGCAACA ACATGAAGCT GCGGCTTTAA ATGCTGTCCA GAGGTTAGAA 
TGGCAGCTCA AACTCCAGGA ACTTGATCCT GCCACCTATA AATCTATCAG CATTTACGAA 
ATCCAGGAGT TTTATGTTCC CCTTGTTGAT GTTAACGACG ACTTTGAATT GACTCCTATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025661.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881150.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X5, mRNA.

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CloneID	OHu50996
Clone ID Related Accession (Same CDS sequence)	XM_005258095.3 , XM_017025662.1 , XM_017025661.1 , XM_017025661.2 , XM_017025662.2 , XM_005258095.2
Accession Version	XM_017025661.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025661.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAATTCC ACACCAAATC ATCCATCACA AACAACGCCT 
GCCCAAAAGA AAACTCCCAG TTCTTCATCT CGACAGAAAG ATAAAGTTAA TAAAAGAAAT 
GAACGTGGTG AAACTCCTTT ACACATGGCT GCTATTCGAG GAGATGTGAA ACAAGTTAAA 
GAATTAATAA GTTTAGGGGC AAATGTGAAT GTGAAAGATT TTGCAGGTTG GACACCACTG 
CATGAAGCTT GCAATGTTGG ATATTACGAT GTTGCTAAGA TACTTATAGC AGCTGGAGCA 
GATGTTAACA CACAAGGATT AGATGATGAC ACTCCACTCC ATGATTCTGC TAGTAGTGGG 
CACAGAGATA TAGTAAAGCT GTTACTTCGT CACGGTGGAA ATCCATTTCA AGCTAATAAA 
CATGGGGAGC GTCCAGTGGA TGTAGCAGAA ACAGAGGAGT TGGAGTTGCT ACTAAAAAGA 
GAGGTGCCTT TATCTGATGA TGATGAAAGT TACACAGATT CCGAAGAGGC TCAATCTGTA 
AATCCTTCTA GTGTTGATGA AAATATTGAC TCTGAAACAG AGAAAGACTC TCTCATCTGT 
GAAAGTAAAC AGATACTTCC CAGTAAAACA CCTCTTCCAT CTGCCCTTGA TGAGTATGAG 
TTCAAAGATG ATGATGATGA AGAAATTAAT AAGATGATTG ATGATAGGCA TATTCTTAGG 
AAAGAACAAC GAAAAGAAAA TGAACCTGAA GCAGAAAAAA CTCATTTATT TGCAAAACAG 
GAGAAAGCCT TCTATCCTAA ATCATTTAAA AGTAAAAAAC AAAAGCCATC TAGGGTCTTA 
TATTCAAGTA CTGAAAGTTC TGATGAAGAA GCTCTTCAGA ATAAAAAGAT TTCTACTTCA 
TGTTCCGTCA TCCCTGAAAC ATCAAATTCT GATATGCAAA CCAAAAAGGA ATATGTAGTT 
TCAGGTGAAC ACAAACAGAA AGGCAAAGTT AAAAGAAAAT TGAAAAATCA GAATAAAAAT 
AAAGAGAACC AAGAGCTAAA GCAAGAAAAG GAAGGAAAAG AAAATACAAG AATAACAAAC 
TTGACAGTAA ATACTGGACT AGATTGTTCA GAAAAGACCA GAGAGGAGGG GAACTTTAGG 
AAATCTTTTA GCCCAAAAGA TGATACTTCA TTACATTTAT TTCATATTTC CACTGGTAAA 
TCTCCCAAAC ATTCTTGTGG ATTAAGTGAA AAACAGTCAA CACCACTAAA ACAAGAACAT 
ACTAAAACAT GTTTATCACC AGGAAGTTCT GAAATGTCAT TACAGCCTGA TCTTGTTCGG 
TATGATAATA CAGAATCTGA ATTCTTGCCA GAAAGTTCAA GTGTAAAATC TTGTAAGCAT 
AAGGAAAAAA GCAAACATCA GAAAGATTTC CACTTAGAAT TTGGTGAAAA ATCAAATGCC 
AAAATAAAGG ATGAAGATCA TAGTCCAACA TTTGAAAATT CAGATTGCAC ACTGAAAAAA 
ATGGATAAAG AAGGTAAAAC ATTAAAAAAA CATAAATTGA AGCATAAAGA GAGGGAAAAA 
GAAAAGCATA AAAAAGAAAT TGAAGGTGAA AAGGAAAAAT ACAAAACTAA GGATAGTGCC 
AAAGAACTGC AGAGGAGTGT GGAATTTGAT AGAGAATTTT GGAAAGAGAA TTTTTTTAAA 
AGTGATGAAA CTGAAGATCT CTTTTTAAAT ATGGAACATG AATCCTTAAC ATTAGAAAAA 
AAATCAAAAT TGGAAAAAAA CATCAAAGAT GATAAATCAA CCAAGGAAAA GCATGTGTCA 
AAAGAGAGGA ACTTTAAAGA GGAACGAGAC AAGATTAAAA AGGAAAGCGA GAAATCTTTT 
AGGGAGGAAA AAATAAAAGA TCTAAAAGAA GAGAGAGAAA ACATACCCAC AGATAAAGAC 
TCAGAATTTA CTTCTTTGGG TATGAGTGCC ATTGAGGAAT CTATAGGGCT TCATTTAGTG 
GAAAAGGAAA TAGACATTGA AAAACAAGAA AAGCATATAA AGGAAAGTAA AGAAAAACCT 
GAGAAGCGAT CTCAAATTAA AGAAAAGGAC ATTGAGAAGA TGGAAAGAAA AACCTTTGAA 
AAAGAAAAGA AGATAAAACA TGAGCATAAG TCAGAAAAAG ACAAATTAGA TCTTAGTGAA 
TGTGTTGATA AAATAAAAGA AAAGGACAAG CTATATTCGC ATCACACAGA AAAATGCCAT 
AAAGAAGGTG AGAAGAGCAA AAATACTGCT GCTATTAAAA AAACTGACGA CAGAGAGAAA 
AGTAGAGAAA AGATGGATAG GAAACATGAC AAAGAAAAGC CTGAAAAAGA GAGGCATCTA 
GCAGAAAGCA AAGAAAAGCA CTTGATGGAG AAAAAAAATA AACAATCAGA TAATAGTGAA 
TACAGTAAAT CAGAAAAAGG CAAAAATAAA GAAAAAGACA GGGAGCTAGA TAAAAAGGAA 
AAATCTAGAG ATAAAGAAAG TATAAATATA ACTAACTCCA AACACATACA GGAAGAAAAA 
AAATCAAGTA TAGTAGACGG TAATAAAGCA CAACATGAAA AACCCTTATC CCTTAAAGAA 
AAAACAAAAG ATGAACCTTT GAAAACTCCA GATGGAAAAG AAAAAGATAA AAAAGATAAA 
GATATAGATA GATACAAAGA ACGAGACAAA CATAAAGATA AAATTCAAAT AAATAGCTTA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCTAAGT CATCACCAGC ATCAAAAGAT 
ACCCGACCTA AAGAAAAGAG GTTAGTGAAT GATGATTTAA TGCAGACAAG TTTTGAACGA 
ATGCTAAGCC TTAAAGACCT AGAAATAGAA CAGTGGCACA AAAAACATAA GGAAAAAATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAC CGAAACTGTT TAGAACTTAA AATAAAAGAT 
AAAGAAAAAA CAAAGCATAC ACCAACTGAA TCCAAAAATA AAGAACTTAC TAGGTCAAAG 
AGTTCAGAAG TGACTGATGC ATATACCAAG GAGAAACAAC CTAAAGATGC TGTAAGTAAC 
AGATCACAAT CTGTTGACAC CAAAAATGTA ATGACTTTAG GGAAGTCATC TTTTGTTTCA 
GATAATAGCT TAAACAGGTC TCCTAGATCA GAAAATGAAA AGCCGGGTCT CAGCTCCAGA 
TCTGTATCCA TGATTTCTGT TGCTAGTTCA GAAGATTCCT GCCATACTAC AGTGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCTGAC TTTATGTTAG AGAGTTCAGA ATCCCAAATG 
TCCTTTTCCC AGTCACCTTT TTTGTCAATT GCCAAATCTC CTGCTCTTCA TGAAAGGGAA 
TTGGACAGCC TGGCTGACTT GCCGGAGCGG ATTAAACCAC CATATGCAAA CAGACTTTCA 
ACATCCCATC TTAGGTCATC TTCTGTAGAA GATGTTAAAC TAATTATAAG CGAGGGGAGA 
CCTACCATAG AAGTTCGAAG ATGTAGCATG CCTTCTGTCA TTTGTGAACA TACCAAACAA 
TTCCAAACAA TATCAGAAGA GAGCAATCAA GGTAGCTTAT TAACTGTGCC AGGAGATACT 
AGTCCTTCTC CCAAACCTGA GGTATTCTCA AATGTGCCTG AAAGAGACCT TTCAAATGTA 
TCTAACATAC ATTCCAGTTT TGCAACTTCT CCAACTGGAG CTTCAAACAG CAAGTATGTT 
TCAGCTGATA GAAATCTCAT CAAGAATACT GCCCCAGTGA ACACTGTAAT GGACAGTCCA 
GTGCATTTAG AGCCATCTAG TCAGGTTGGT GTGATCCAGA ATAAATCATG GGAGATGCCT 
GTTGATAGAC TAGAGACATT AAGCACCAGA GACTTTATCT GCCCAAATTC TAACATACCT 
GATCAAGAAT CCTCTCTTCA GAGTTTTTGT AATTCTGAAA ATAAGGTATT GAAAGAAAAT 
GCTGATTTTT TATCCCTGCG CCAGACTGAA CTGCCAGGAA ACTCTTGTGC TCAGGATCCG 
GCATCCTTTA TGCCTCCACA GCAGCCTTGC TCTTTCCCCA GCCAATCACT TTCAGATGCT 
GAATCGATTT CTAAACATAT GTCTTTGTCA TATGTTGCTA ATCAAGAGCC AGGTATTTTA 
CAACAAAAAA ATGCAGTTCA GATTATTAGT TCTGCTTTAG ATACTGATAA TGAATCTACA 
AAAGATACAG AAAATACTTT TGTCCTAGGA GATGTTCAAA AAACAGATGC CTTTGTCCCA 
GTGTACTCTG ACAGCACTAT TCAAGAAGCA TCACCAAACT TTGAGAAAGC TTATACTTTA 
CCTGTGTTAC CATCAGAAAA GGACTTTAAT GGAAGTGATG CCTCTACCCA GCTAAATACA 
CATTATGCAT TTAGCAAACT AACTTACAAG TCTTCCAGTG GCCATGAAGT TGAGAATAGC 
ACAACTGATA CTCAGGTCAT TTCACATGAA AAAGAAAACA AACTGGAGAG TTTGGTTTTA 
ACTCATTTGA GTAGGTGTGA TTCTGATTTA TGTGAAATGA ATGCAGGGAT GCCAAAAGGA 
AACCTAAATG AACAAGATCC AAAACATTGT CCTGAAAGTG AAAAGTGTTT GCTTTCCATA 
GAAGATGAGG AATCTCAACA AAGCATTTTA TCAAGTCTGG AAAACCATTC ACAGCAGTCA 
ACTCAACCAG AAATGCATAA ATATGGTCAG TTAGTTAAAG TAGAATTAGA AGAAAATGCC 
GAAGATGATA AAACTGAAAA CCAAATCCCT CAAAGAATGA CTAGAAACAA AGCAAATACA 
ATGGCAAATC AAAGCAAACA GATTCTTGCT AGCTGTACAC TATTATCAGA AAAAGACAGT 
GAATCCTCAT CTCCTAGAGG AAGAATAAGA TTAACTGAAG ATGACGATCC TCAAATTCAC 
CATCCACGGA AAAGGAAAGT GTCACGTGTA CCTCAGCCTG TGCAAGTGAG TCCCTCTTTA 
CTACAAGCAA AAGAGAAAAC TCAGCAATCT CTGGCAGCCA TTGTAGATTC TCTAAAACTA 
GATGAGATTC AGCCATACAG TTCAGAGAGA GCAAATCCAT ATTTTGAATA CTTGCACATA 
AGGAAAAAAA TAGAAGAAAA ACGCAAATTA CTGTGTAGTG TGATTCCTCA AGCACCTCAG 
TACTATGACG AATATGTAAC ATTTAACGGA TCATATCTCC TGGATGGAAA CCCCTTAAGC 
AAGATTTGTA TTCCCACAAT TACACCACCA CCTTCACTGT CAGATCCACT TAAAGAGCTT 
TTTCGACAAC AGGAAGTTGT AAGGATGAAA CTACGTTTGC AACACAGTAT TGAAAGGGAA 
AAACTCATTG TATCCAACGA ACAAGAAGTT TTGCGAGTTC ATTACAGAGC TGCAAGAACA 
TTGGCAAATC AAACACTGCC ATTTAGTGCT TGTACTGTTT TGCTGGATGC CGAAGTATAC 
AATGTACCAT TGGACTCTCA GTCTGATGAC AGTAAAACTT CTGTGAGGGA TCGCTTTAAT 
GCAAGACAAT TCATGTCTTG GTTACAAGAT GTGGATGATA AATTTGACAA ATTAAAGACC 
TGTCTTTTAA TGAGGCAACA ACATGAAGCT GCGGCTTTAA ATGCTGTCCA GAGGTTAGAA 
TGGCAGCTCA AACTCCAGGA ACTTGATCCT GCCACCTATA AATCTATCAG CATTTACGAA 
ATCCAGGAGT TTTATGTTCC CCTTGTTGAT GTTAACGACG ACTTTGAATT GACTCCTATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881150.1
CDS	366..6548
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNNSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025661.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025661.2

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAATTCC ACACCAAATC ATCCATCACA AACAACGCCT 
GCCCAAAAGA AAACTCCCAG TTCTTCATCT CGACAGAAAG ATAAAGTTAA TAAAAGAAAT 
GAACGTGGTG AAACTCCTTT ACACATGGCT GCTATTCGAG GAGATGTGAA ACAAGTTAAA 
GAATTAATAA GTTTAGGGGC AAATGTGAAT GTGAAAGATT TTGCAGGTTG GACACCACTG 
CATGAAGCTT GCAATGTTGG ATATTACGAT GTTGCTAAGA TACTTATAGC AGCTGGAGCA 
GATGTTAACA CACAAGGATT AGATGATGAC ACTCCACTCC ATGATTCTGC TAGTAGTGGG 
CACAGAGATA TAGTAAAGCT GTTACTTCGT CACGGTGGAA ATCCATTTCA AGCTAATAAA 
CATGGGGAGC GTCCAGTGGA TGTAGCAGAA ACAGAGGAGT TGGAGTTGCT ACTAAAAAGA 
GAGGTGCCTT TATCTGATGA TGATGAAAGT TACACAGATT CCGAAGAGGC TCAATCTGTA 
AATCCTTCTA GTGTTGATGA AAATATTGAC TCTGAAACAG AGAAAGACTC TCTCATCTGT 
GAAAGTAAAC AGATACTTCC CAGTAAAACA CCTCTTCCAT CTGCCCTTGA TGAGTATGAG 
TTCAAAGATG ATGATGATGA AGAAATTAAT AAGATGATTG ATGATAGGCA TATTCTTAGG 
AAAGAACAAC GAAAAGAAAA TGAACCTGAA GCAGAAAAAA CTCATTTATT TGCAAAACAG 
GAGAAAGCCT TCTATCCTAA ATCATTTAAA AGTAAAAAAC AAAAGCCATC TAGGGTCTTA 
TATTCAAGTA CTGAAAGTTC TGATGAAGAA GCTCTTCAGA ATAAAAAGAT TTCTACTTCA 
TGTTCCGTCA TCCCTGAAAC ATCAAATTCT GATATGCAAA CCAAAAAGGA ATATGTAGTT 
TCAGGTGAAC ACAAACAGAA AGGCAAAGTT AAAAGAAAAT TGAAAAATCA GAATAAAAAT 
AAAGAGAACC AAGAGCTAAA GCAAGAAAAG GAAGGAAAAG AAAATACAAG AATAACAAAC 
TTGACAGTAA ATACTGGACT AGATTGTTCA GAAAAGACCA GAGAGGAGGG GAACTTTAGG 
AAATCTTTTA GCCCAAAAGA TGATACTTCA TTACATTTAT TTCATATTTC CACTGGTAAA 
TCTCCCAAAC ATTCTTGTGG ATTAAGTGAA AAACAGTCAA CACCACTAAA ACAAGAACAT 
ACTAAAACAT GTTTATCACC AGGAAGTTCT GAAATGTCAT TACAGCCTGA TCTTGTTCGG 
TATGATAATA CAGAATCTGA ATTCTTGCCA GAAAGTTCAA GTGTAAAATC TTGTAAGCAT 
AAGGAAAAAA GCAAACATCA GAAAGATTTC CACTTAGAAT TTGGTGAAAA ATCAAATGCC 
AAAATAAAGG ATGAAGATCA TAGTCCAACA TTTGAAAATT CAGATTGCAC ACTGAAAAAA 
ATGGATAAAG AAGGTAAAAC ATTAAAAAAA CATAAATTGA AGCATAAAGA GAGGGAAAAA 
GAAAAGCATA AAAAAGAAAT TGAAGGTGAA AAGGAAAAAT ACAAAACTAA GGATAGTGCC 
AAAGAACTGC AGAGGAGTGT GGAATTTGAT AGAGAATTTT GGAAAGAGAA TTTTTTTAAA 
AGTGATGAAA CTGAAGATCT CTTTTTAAAT ATGGAACATG AATCCTTAAC ATTAGAAAAA 
AAATCAAAAT TGGAAAAAAA CATCAAAGAT GATAAATCAA CCAAGGAAAA GCATGTGTCA 
AAAGAGAGGA ACTTTAAAGA GGAACGAGAC AAGATTAAAA AGGAAAGCGA GAAATCTTTT 
AGGGAGGAAA AAATAAAAGA TCTAAAAGAA GAGAGAGAAA ACATACCCAC AGATAAAGAC 
TCAGAATTTA CTTCTTTGGG TATGAGTGCC ATTGAGGAAT CTATAGGGCT TCATTTAGTG 
GAAAAGGAAA TAGACATTGA AAAACAAGAA AAGCATATAA AGGAAAGTAA AGAAAAACCT 
GAGAAGCGAT CTCAAATTAA AGAAAAGGAC ATTGAGAAGA TGGAAAGAAA AACCTTTGAA 
AAAGAAAAGA AGATAAAACA TGAGCATAAG TCAGAAAAAG ACAAATTAGA TCTTAGTGAA 
TGTGTTGATA AAATAAAAGA AAAGGACAAG CTATATTCGC ATCACACAGA AAAATGCCAT 
AAAGAAGGTG AGAAGAGCAA AAATACTGCT GCTATTAAAA AAACTGACGA CAGAGAGAAA 
AGTAGAGAAA AGATGGATAG GAAACATGAC AAAGAAAAGC CTGAAAAAGA GAGGCATCTA 
GCAGAAAGCA AAGAAAAGCA CTTGATGGAG AAAAAAAATA AACAATCAGA TAATAGTGAA 
TACAGTAAAT CAGAAAAAGG CAAAAATAAA GAAAAAGACA GGGAGCTAGA TAAAAAGGAA 
AAATCTAGAG ATAAAGAAAG TATAAATATA ACTAACTCCA AACACATACA GGAAGAAAAA 
AAATCAAGTA TAGTAGACGG TAATAAAGCA CAACATGAAA AACCCTTATC CCTTAAAGAA 
AAAACAAAAG ATGAACCTTT GAAAACTCCA GATGGAAAAG AAAAAGATAA AAAAGATAAA 
GATATAGATA GATACAAAGA ACGAGACAAA CATAAAGATA AAATTCAAAT AAATAGCTTA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCTAAGT CATCACCAGC ATCAAAAGAT 
ACCCGACCTA AAGAAAAGAG GTTAGTGAAT GATGATTTAA TGCAGACAAG TTTTGAACGA 
ATGCTAAGCC TTAAAGACCT AGAAATAGAA CAGTGGCACA AAAAACATAA GGAAAAAATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAC CGAAACTGTT TAGAACTTAA AATAAAAGAT 
AAAGAAAAAA CAAAGCATAC ACCAACTGAA TCCAAAAATA AAGAACTTAC TAGGTCAAAG 
AGTTCAGAAG TGACTGATGC ATATACCAAG GAGAAACAAC CTAAAGATGC TGTAAGTAAC 
AGATCACAAT CTGTTGACAC CAAAAATGTA ATGACTTTAG GGAAGTCATC TTTTGTTTCA 
GATAATAGCT TAAACAGGTC TCCTAGATCA GAAAATGAAA AGCCGGGTCT CAGCTCCAGA 
TCTGTATCCA TGATTTCTGT TGCTAGTTCA GAAGATTCCT GCCATACTAC AGTGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCTGAC TTTATGTTAG AGAGTTCAGA ATCCCAAATG 
TCCTTTTCCC AGTCACCTTT TTTGTCAATT GCCAAATCTC CTGCTCTTCA TGAAAGGGAA 
TTGGACAGCC TGGCTGACTT GCCGGAGCGG ATTAAACCAC CATATGCAAA CAGACTTTCA 
ACATCCCATC TTAGGTCATC TTCTGTAGAA GATGTTAAAC TAATTATAAG CGAGGGGAGA 
CCTACCATAG AAGTTCGAAG ATGTAGCATG CCTTCTGTCA TTTGTGAACA TACCAAACAA 
TTCCAAACAA TATCAGAAGA GAGCAATCAA GGTAGCTTAT TAACTGTGCC AGGAGATACT 
AGTCCTTCTC CCAAACCTGA GGTATTCTCA AATGTGCCTG AAAGAGACCT TTCAAATGTA 
TCTAACATAC ATTCCAGTTT TGCAACTTCT CCAACTGGAG CTTCAAACAG CAAGTATGTT 
TCAGCTGATA GAAATCTCAT CAAGAATACT GCCCCAGTGA ACACTGTAAT GGACAGTCCA 
GTGCATTTAG AGCCATCTAG TCAGGTTGGT GTGATCCAGA ATAAATCATG GGAGATGCCT 
GTTGATAGAC TAGAGACATT AAGCACCAGA GACTTTATCT GCCCAAATTC TAACATACCT 
GATCAAGAAT CCTCTCTTCA GAGTTTTTGT AATTCTGAAA ATAAGGTATT GAAAGAAAAT 
GCTGATTTTT TATCCCTGCG CCAGACTGAA CTGCCAGGAA ACTCTTGTGC TCAGGATCCG 
GCATCCTTTA TGCCTCCACA GCAGCCTTGC TCTTTCCCCA GCCAATCACT TTCAGATGCT 
GAATCGATTT CTAAACATAT GTCTTTGTCA TATGTTGCTA ATCAAGAGCC AGGTATTTTA 
CAACAAAAAA ATGCAGTTCA GATTATTAGT TCTGCTTTAG ATACTGATAA TGAATCTACA 
AAAGATACAG AAAATACTTT TGTCCTAGGA GATGTTCAAA AAACAGATGC CTTTGTCCCA 
GTGTACTCTG ACAGCACTAT TCAAGAAGCA TCACCAAACT TTGAGAAAGC TTATACTTTA 
CCTGTGTTAC CATCAGAAAA GGACTTTAAT GGAAGTGATG CCTCTACCCA GCTAAATACA 
CATTATGCAT TTAGCAAACT AACTTACAAG TCTTCCAGTG GCCATGAAGT TGAGAATAGC 
ACAACTGATA CTCAGGTCAT TTCACATGAA AAAGAAAACA AACTGGAGAG TTTGGTTTTA 
ACTCATTTGA GTAGGTGTGA TTCTGATTTA TGTGAAATGA ATGCAGGGAT GCCAAAAGGA 
AACCTAAATG AACAAGATCC AAAACATTGT CCTGAAAGTG AAAAGTGTTT GCTTTCCATA 
GAAGATGAGG AATCTCAACA AAGCATTTTA TCAAGTCTGG AAAACCATTC ACAGCAGTCA 
ACTCAACCAG AAATGCATAA ATATGGTCAG TTAGTTAAAG TAGAATTAGA AGAAAATGCC 
GAAGATGATA AAACTGAAAA CCAAATCCCT CAAAGAATGA CTAGAAACAA AGCAAATACA 
ATGGCAAATC AAAGCAAACA GATTCTTGCT AGCTGTACAC TATTATCAGA AAAAGACAGT 
GAATCCTCAT CTCCTAGAGG AAGAATAAGA TTAACTGAAG ATGACGATCC TCAAATTCAC 
CATCCACGGA AAAGGAAAGT GTCACGTGTA CCTCAGCCTG TGCAAGTGAG TCCCTCTTTA 
CTACAAGCAA AAGAGAAAAC TCAGCAATCT CTGGCAGCCA TTGTAGATTC TCTAAAACTA 
GATGAGATTC AGCCATACAG TTCAGAGAGA GCAAATCCAT ATTTTGAATA CTTGCACATA 
AGGAAAAAAA TAGAAGAAAA ACGCAAATTA CTGTGTAGTG TGATTCCTCA AGCACCTCAG 
TACTATGACG AATATGTAAC ATTTAACGGA TCATATCTCC TGGATGGAAA CCCCTTAAGC 
AAGATTTGTA TTCCCACAAT TACACCACCA CCTTCACTGT CAGATCCACT TAAAGAGCTT 
TTTCGACAAC AGGAAGTTGT AAGGATGAAA CTACGTTTGC AACACAGTAT TGAAAGGGAA 
AAACTCATTG TATCCAACGA ACAAGAAGTT TTGCGAGTTC ATTACAGAGC TGCAAGAACA 
TTGGCAAATC AAACACTGCC ATTTAGTGCT TGTACTGTTT TGCTGGATGC CGAAGTATAC 
AATGTACCAT TGGACTCTCA GTCTGATGAC AGTAAAACTT CTGTGAGGGA TCGCTTTAAT 
GCAAGACAAT TCATGTCTTG GTTACAAGAT GTGGATGATA AATTTGACAA ATTAAAGACC 
TGTCTTTTAA TGAGGCAACA ACATGAAGCT GCGGCTTTAA ATGCTGTCCA GAGGTTAGAA 
TGGCAGCTCA AACTCCAGGA ACTTGATCCT GCCACCTATA AATCTATCAG CATTTACGAA 
ATCCAGGAGT TTTATGTTCC CCTTGTTGAT GTTAACGACG ACTTTGAATT GACTCCTATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025662.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881151.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X6, mRNA.

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CloneID	OHu50996
Clone ID Related Accession (Same CDS sequence)	XM_005258095.3 , XM_017025662.1 , XM_017025661.1 , XM_017025661.2 , XM_017025662.2 , XM_005258095.2
Accession Version	XM_017025662.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025662.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAATTCC ACACCAAATC ATCCATCACA AACAACGCCT 
GCCCAAAAGA AAACTCCCAG TTCTTCATCT CGACAGAAAG ATAAAGTTAA TAAAAGAAAT 
GAACGTGGTG AAACTCCTTT ACACATGGCT GCTATTCGAG GAGATGTGAA ACAAGTTAAA 
GAATTAATAA GTTTAGGGGC AAATGTGAAT GTGAAAGATT TTGCAGGTTG GACACCACTG 
CATGAAGCTT GCAATGTTGG ATATTACGAT GTTGCTAAGA TACTTATAGC AGCTGGAGCA 
GATGTTAACA CACAAGGATT AGATGATGAC ACTCCACTCC ATGATTCTGC TAGTAGTGGG 
CACAGAGATA TAGTAAAGCT GTTACTTCGT CACGGTGGAA ATCCATTTCA AGCTAATAAA 
CATGGGGAGC GTCCAGTGGA TGTAGCAGAA ACAGAGGAGT TGGAGTTGCT ACTAAAAAGA 
GAGGTGCCTT TATCTGATGA TGATGAAAGT TACACAGATT CCGAAGAGGC TCAATCTGTA 
AATCCTTCTA GTGTTGATGA AAATATTGAC TCTGAAACAG AGAAAGACTC TCTCATCTGT 
GAAAGTAAAC AGATACTTCC CAGTAAAACA CCTCTTCCAT CTGCCCTTGA TGAGTATGAG 
TTCAAAGATG ATGATGATGA AGAAATTAAT AAGATGATTG ATGATAGGCA TATTCTTAGG 
AAAGAACAAC GAAAAGAAAA TGAACCTGAA GCAGAAAAAA CTCATTTATT TGCAAAACAG 
GAGAAAGCCT TCTATCCTAA ATCATTTAAA AGTAAAAAAC AAAAGCCATC TAGGGTCTTA 
TATTCAAGTA CTGAAAGTTC TGATGAAGAA GCTCTTCAGA ATAAAAAGAT TTCTACTTCA 
TGTTCCGTCA TCCCTGAAAC ATCAAATTCT GATATGCAAA CCAAAAAGGA ATATGTAGTT 
TCAGGTGAAC ACAAACAGAA AGGCAAAGTT AAAAGAAAAT TGAAAAATCA GAATAAAAAT 
AAAGAGAACC AAGAGCTAAA GCAAGAAAAG GAAGGAAAAG AAAATACAAG AATAACAAAC 
TTGACAGTAA ATACTGGACT AGATTGTTCA GAAAAGACCA GAGAGGAGGG GAACTTTAGG 
AAATCTTTTA GCCCAAAAGA TGATACTTCA TTACATTTAT TTCATATTTC CACTGGTAAA 
TCTCCCAAAC ATTCTTGTGG ATTAAGTGAA AAACAGTCAA CACCACTAAA ACAAGAACAT 
ACTAAAACAT GTTTATCACC AGGAAGTTCT GAAATGTCAT TACAGCCTGA TCTTGTTCGG 
TATGATAATA CAGAATCTGA ATTCTTGCCA GAAAGTTCAA GTGTAAAATC TTGTAAGCAT 
AAGGAAAAAA GCAAACATCA GAAAGATTTC CACTTAGAAT TTGGTGAAAA ATCAAATGCC 
AAAATAAAGG ATGAAGATCA TAGTCCAACA TTTGAAAATT CAGATTGCAC ACTGAAAAAA 
ATGGATAAAG AAGGTAAAAC ATTAAAAAAA CATAAATTGA AGCATAAAGA GAGGGAAAAA 
GAAAAGCATA AAAAAGAAAT TGAAGGTGAA AAGGAAAAAT ACAAAACTAA GGATAGTGCC 
AAAGAACTGC AGAGGAGTGT GGAATTTGAT AGAGAATTTT GGAAAGAGAA TTTTTTTAAA 
AGTGATGAAA CTGAAGATCT CTTTTTAAAT ATGGAACATG AATCCTTAAC ATTAGAAAAA 
AAATCAAAAT TGGAAAAAAA CATCAAAGAT GATAAATCAA CCAAGGAAAA GCATGTGTCA 
AAAGAGAGGA ACTTTAAAGA GGAACGAGAC AAGATTAAAA AGGAAAGCGA GAAATCTTTT 
AGGGAGGAAA AAATAAAAGA TCTAAAAGAA GAGAGAGAAA ACATACCCAC AGATAAAGAC 
TCAGAATTTA CTTCTTTGGG TATGAGTGCC ATTGAGGAAT CTATAGGGCT TCATTTAGTG 
GAAAAGGAAA TAGACATTGA AAAACAAGAA AAGCATATAA AGGAAAGTAA AGAAAAACCT 
GAGAAGCGAT CTCAAATTAA AGAAAAGGAC ATTGAGAAGA TGGAAAGAAA AACCTTTGAA 
AAAGAAAAGA AGATAAAACA TGAGCATAAG TCAGAAAAAG ACAAATTAGA TCTTAGTGAA 
TGTGTTGATA AAATAAAAGA AAAGGACAAG CTATATTCGC ATCACACAGA AAAATGCCAT 
AAAGAAGGTG AGAAGAGCAA AAATACTGCT GCTATTAAAA AAACTGACGA CAGAGAGAAA 
AGTAGAGAAA AGATGGATAG GAAACATGAC AAAGAAAAGC CTGAAAAAGA GAGGCATCTA 
GCAGAAAGCA AAGAAAAGCA CTTGATGGAG AAAAAAAATA AACAATCAGA TAATAGTGAA 
TACAGTAAAT CAGAAAAAGG CAAAAATAAA GAAAAAGACA GGGAGCTAGA TAAAAAGGAA 
AAATCTAGAG ATAAAGAAAG TATAAATATA ACTAACTCCA AACACATACA GGAAGAAAAA 
AAATCAAGTA TAGTAGACGG TAATAAAGCA CAACATGAAA AACCCTTATC CCTTAAAGAA 
AAAACAAAAG ATGAACCTTT GAAAACTCCA GATGGAAAAG AAAAAGATAA AAAAGATAAA 
GATATAGATA GATACAAAGA ACGAGACAAA CATAAAGATA AAATTCAAAT AAATAGCTTA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCTAAGT CATCACCAGC ATCAAAAGAT 
ACCCGACCTA AAGAAAAGAG GTTAGTGAAT GATGATTTAA TGCAGACAAG TTTTGAACGA 
ATGCTAAGCC TTAAAGACCT AGAAATAGAA CAGTGGCACA AAAAACATAA GGAAAAAATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAC CGAAACTGTT TAGAACTTAA AATAAAAGAT 
AAAGAAAAAA CAAAGCATAC ACCAACTGAA TCCAAAAATA AAGAACTTAC TAGGTCAAAG 
AGTTCAGAAG TGACTGATGC ATATACCAAG GAGAAACAAC CTAAAGATGC TGTAAGTAAC 
AGATCACAAT CTGTTGACAC CAAAAATGTA ATGACTTTAG GGAAGTCATC TTTTGTTTCA 
GATAATAGCT TAAACAGGTC TCCTAGATCA GAAAATGAAA AGCCGGGTCT CAGCTCCAGA 
TCTGTATCCA TGATTTCTGT TGCTAGTTCA GAAGATTCCT GCCATACTAC AGTGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCTGAC TTTATGTTAG AGAGTTCAGA ATCCCAAATG 
TCCTTTTCCC AGTCACCTTT TTTGTCAATT GCCAAATCTC CTGCTCTTCA TGAAAGGGAA 
TTGGACAGCC TGGCTGACTT GCCGGAGCGG ATTAAACCAC CATATGCAAA CAGACTTTCA 
ACATCCCATC TTAGGTCATC TTCTGTAGAA GATGTTAAAC TAATTATAAG CGAGGGGAGA 
CCTACCATAG AAGTTCGAAG ATGTAGCATG CCTTCTGTCA TTTGTGAACA TACCAAACAA 
TTCCAAACAA TATCAGAAGA GAGCAATCAA GGTAGCTTAT TAACTGTGCC AGGAGATACT 
AGTCCTTCTC CCAAACCTGA GGTATTCTCA AATGTGCCTG AAAGAGACCT TTCAAATGTA 
TCTAACATAC ATTCCAGTTT TGCAACTTCT CCAACTGGAG CTTCAAACAG CAAGTATGTT 
TCAGCTGATA GAAATCTCAT CAAGAATACT GCCCCAGTGA ACACTGTAAT GGACAGTCCA 
GTGCATTTAG AGCCATCTAG TCAGGTTGGT GTGATCCAGA ATAAATCATG GGAGATGCCT 
GTTGATAGAC TAGAGACATT AAGCACCAGA GACTTTATCT GCCCAAATTC TAACATACCT 
GATCAAGAAT CCTCTCTTCA GAGTTTTTGT AATTCTGAAA ATAAGGTATT GAAAGAAAAT 
GCTGATTTTT TATCCCTGCG CCAGACTGAA CTGCCAGGAA ACTCTTGTGC TCAGGATCCG 
GCATCCTTTA TGCCTCCACA GCAGCCTTGC TCTTTCCCCA GCCAATCACT TTCAGATGCT 
GAATCGATTT CTAAACATAT GTCTTTGTCA TATGTTGCTA ATCAAGAGCC AGGTATTTTA 
CAACAAAAAA ATGCAGTTCA GATTATTAGT TCTGCTTTAG ATACTGATAA TGAATCTACA 
AAAGATACAG AAAATACTTT TGTCCTAGGA GATGTTCAAA AAACAGATGC CTTTGTCCCA 
GTGTACTCTG ACAGCACTAT TCAAGAAGCA TCACCAAACT TTGAGAAAGC TTATACTTTA 
CCTGTGTTAC CATCAGAAAA GGACTTTAAT GGAAGTGATG CCTCTACCCA GCTAAATACA 
CATTATGCAT TTAGCAAACT AACTTACAAG TCTTCCAGTG GCCATGAAGT TGAGAATAGC 
ACAACTGATA CTCAGGTCAT TTCACATGAA AAAGAAAACA AACTGGAGAG TTTGGTTTTA 
ACTCATTTGA GTAGGTGTGA TTCTGATTTA TGTGAAATGA ATGCAGGGAT GCCAAAAGGA 
AACCTAAATG AACAAGATCC AAAACATTGT CCTGAAAGTG AAAAGTGTTT GCTTTCCATA 
GAAGATGAGG AATCTCAACA AAGCATTTTA TCAAGTCTGG AAAACCATTC ACAGCAGTCA 
ACTCAACCAG AAATGCATAA ATATGGTCAG TTAGTTAAAG TAGAATTAGA AGAAAATGCC 
GAAGATGATA AAACTGAAAA CCAAATCCCT CAAAGAATGA CTAGAAACAA AGCAAATACA 
ATGGCAAATC AAAGCAAACA GATTCTTGCT AGCTGTACAC TATTATCAGA AAAAGACAGT 
GAATCCTCAT CTCCTAGAGG AAGAATAAGA TTAACTGAAG ATGACGATCC TCAAATTCAC 
CATCCACGGA AAAGGAAAGT GTCACGTGTA CCTCAGCCTG TGCAAGTGAG TCCCTCTTTA 
CTACAAGCAA AAGAGAAAAC TCAGCAATCT CTGGCAGCCA TTGTAGATTC TCTAAAACTA 
GATGAGATTC AGCCATACAG TTCAGAGAGA GCAAATCCAT ATTTTGAATA CTTGCACATA 
AGGAAAAAAA TAGAAGAAAA ACGCAAATTA CTGTGTAGTG TGATTCCTCA AGCACCTCAG 
TACTATGACG AATATGTAAC ATTTAACGGA TCATATCTCC TGGATGGAAA CCCCTTAAGC 
AAGATTTGTA TTCCCACAAT TACACCACCA CCTTCACTGT CAGATCCACT TAAAGAGCTT 
TTTCGACAAC AGGAAGTTGT AAGGATGAAA CTACGTTTGC AACACAGTAT TGAAAGGGAA 
AAACTCATTG TATCCAACGA ACAAGAAGTT TTGCGAGTTC ATTACAGAGC TGCAAGAACA 
TTGGCAAATC AAACACTGCC ATTTAGTGCT TGTACTGTTT TGCTGGATGC CGAAGTATAC 
AATGTACCAT TGGACTCTCA GTCTGATGAC AGTAAAACTT CTGTGAGGGA TCGCTTTAAT 
GCAAGACAAT TCATGTCTTG GTTACAAGAT GTGGATGATA AATTTGACAA ATTAAAGACC 
TGTCTTTTAA TGAGGCAACA ACATGAAGCT GCGGCTTTAA ATGCTGTCCA GAGGTTAGAA 
TGGCAGCTCA AACTCCAGGA ACTTGATCCT GCCACCTATA AATCTATCAG CATTTACGAA 
ATCCAGGAGT TTTATGTTCC CCTTGTTGAT GTTAACGACG ACTTTGAATT GACTCCTATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881151.1
CDS	411..6593
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNNSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025662.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025662.2

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAATTCC ACACCAAATC ATCCATCACA AACAACGCCT 
GCCCAAAAGA AAACTCCCAG TTCTTCATCT CGACAGAAAG ATAAAGTTAA TAAAAGAAAT 
GAACGTGGTG AAACTCCTTT ACACATGGCT GCTATTCGAG GAGATGTGAA ACAAGTTAAA 
GAATTAATAA GTTTAGGGGC AAATGTGAAT GTGAAAGATT TTGCAGGTTG GACACCACTG 
CATGAAGCTT GCAATGTTGG ATATTACGAT GTTGCTAAGA TACTTATAGC AGCTGGAGCA 
GATGTTAACA CACAAGGATT AGATGATGAC ACTCCACTCC ATGATTCTGC TAGTAGTGGG 
CACAGAGATA TAGTAAAGCT GTTACTTCGT CACGGTGGAA ATCCATTTCA AGCTAATAAA 
CATGGGGAGC GTCCAGTGGA TGTAGCAGAA ACAGAGGAGT TGGAGTTGCT ACTAAAAAGA 
GAGGTGCCTT TATCTGATGA TGATGAAAGT TACACAGATT CCGAAGAGGC TCAATCTGTA 
AATCCTTCTA GTGTTGATGA AAATATTGAC TCTGAAACAG AGAAAGACTC TCTCATCTGT 
GAAAGTAAAC AGATACTTCC CAGTAAAACA CCTCTTCCAT CTGCCCTTGA TGAGTATGAG 
TTCAAAGATG ATGATGATGA AGAAATTAAT AAGATGATTG ATGATAGGCA TATTCTTAGG 
AAAGAACAAC GAAAAGAAAA TGAACCTGAA GCAGAAAAAA CTCATTTATT TGCAAAACAG 
GAGAAAGCCT TCTATCCTAA ATCATTTAAA AGTAAAAAAC AAAAGCCATC TAGGGTCTTA 
TATTCAAGTA CTGAAAGTTC TGATGAAGAA GCTCTTCAGA ATAAAAAGAT TTCTACTTCA 
TGTTCCGTCA TCCCTGAAAC ATCAAATTCT GATATGCAAA CCAAAAAGGA ATATGTAGTT 
TCAGGTGAAC ACAAACAGAA AGGCAAAGTT AAAAGAAAAT TGAAAAATCA GAATAAAAAT 
AAAGAGAACC AAGAGCTAAA GCAAGAAAAG GAAGGAAAAG AAAATACAAG AATAACAAAC 
TTGACAGTAA ATACTGGACT AGATTGTTCA GAAAAGACCA GAGAGGAGGG GAACTTTAGG 
AAATCTTTTA GCCCAAAAGA TGATACTTCA TTACATTTAT TTCATATTTC CACTGGTAAA 
TCTCCCAAAC ATTCTTGTGG ATTAAGTGAA AAACAGTCAA CACCACTAAA ACAAGAACAT 
ACTAAAACAT GTTTATCACC AGGAAGTTCT GAAATGTCAT TACAGCCTGA TCTTGTTCGG 
TATGATAATA CAGAATCTGA ATTCTTGCCA GAAAGTTCAA GTGTAAAATC TTGTAAGCAT 
AAGGAAAAAA GCAAACATCA GAAAGATTTC CACTTAGAAT TTGGTGAAAA ATCAAATGCC 
AAAATAAAGG ATGAAGATCA TAGTCCAACA TTTGAAAATT CAGATTGCAC ACTGAAAAAA 
ATGGATAAAG AAGGTAAAAC ATTAAAAAAA CATAAATTGA AGCATAAAGA GAGGGAAAAA 
GAAAAGCATA AAAAAGAAAT TGAAGGTGAA AAGGAAAAAT ACAAAACTAA GGATAGTGCC 
AAAGAACTGC AGAGGAGTGT GGAATTTGAT AGAGAATTTT GGAAAGAGAA TTTTTTTAAA 
AGTGATGAAA CTGAAGATCT CTTTTTAAAT ATGGAACATG AATCCTTAAC ATTAGAAAAA 
AAATCAAAAT TGGAAAAAAA CATCAAAGAT GATAAATCAA CCAAGGAAAA GCATGTGTCA 
AAAGAGAGGA ACTTTAAAGA GGAACGAGAC AAGATTAAAA AGGAAAGCGA GAAATCTTTT 
AGGGAGGAAA AAATAAAAGA TCTAAAAGAA GAGAGAGAAA ACATACCCAC AGATAAAGAC 
TCAGAATTTA CTTCTTTGGG TATGAGTGCC ATTGAGGAAT CTATAGGGCT TCATTTAGTG 
GAAAAGGAAA TAGACATTGA AAAACAAGAA AAGCATATAA AGGAAAGTAA AGAAAAACCT 
GAGAAGCGAT CTCAAATTAA AGAAAAGGAC ATTGAGAAGA TGGAAAGAAA AACCTTTGAA 
AAAGAAAAGA AGATAAAACA TGAGCATAAG TCAGAAAAAG ACAAATTAGA TCTTAGTGAA 
TGTGTTGATA AAATAAAAGA AAAGGACAAG CTATATTCGC ATCACACAGA AAAATGCCAT 
AAAGAAGGTG AGAAGAGCAA AAATACTGCT GCTATTAAAA AAACTGACGA CAGAGAGAAA 
AGTAGAGAAA AGATGGATAG GAAACATGAC AAAGAAAAGC CTGAAAAAGA GAGGCATCTA 
GCAGAAAGCA AAGAAAAGCA CTTGATGGAG AAAAAAAATA AACAATCAGA TAATAGTGAA 
TACAGTAAAT CAGAAAAAGG CAAAAATAAA GAAAAAGACA GGGAGCTAGA TAAAAAGGAA 
AAATCTAGAG ATAAAGAAAG TATAAATATA ACTAACTCCA AACACATACA GGAAGAAAAA 
AAATCAAGTA TAGTAGACGG TAATAAAGCA CAACATGAAA AACCCTTATC CCTTAAAGAA 
AAAACAAAAG ATGAACCTTT GAAAACTCCA GATGGAAAAG AAAAAGATAA AAAAGATAAA 
GATATAGATA GATACAAAGA ACGAGACAAA CATAAAGATA AAATTCAAAT AAATAGCTTA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCTAAGT CATCACCAGC ATCAAAAGAT 
ACCCGACCTA AAGAAAAGAG GTTAGTGAAT GATGATTTAA TGCAGACAAG TTTTGAACGA 
ATGCTAAGCC TTAAAGACCT AGAAATAGAA CAGTGGCACA AAAAACATAA GGAAAAAATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAC CGAAACTGTT TAGAACTTAA AATAAAAGAT 
AAAGAAAAAA CAAAGCATAC ACCAACTGAA TCCAAAAATA AAGAACTTAC TAGGTCAAAG 
AGTTCAGAAG TGACTGATGC ATATACCAAG GAGAAACAAC CTAAAGATGC TGTAAGTAAC 
AGATCACAAT CTGTTGACAC CAAAAATGTA ATGACTTTAG GGAAGTCATC TTTTGTTTCA 
GATAATAGCT TAAACAGGTC TCCTAGATCA GAAAATGAAA AGCCGGGTCT CAGCTCCAGA 
TCTGTATCCA TGATTTCTGT TGCTAGTTCA GAAGATTCCT GCCATACTAC AGTGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCTGAC TTTATGTTAG AGAGTTCAGA ATCCCAAATG 
TCCTTTTCCC AGTCACCTTT TTTGTCAATT GCCAAATCTC CTGCTCTTCA TGAAAGGGAA 
TTGGACAGCC TGGCTGACTT GCCGGAGCGG ATTAAACCAC CATATGCAAA CAGACTTTCA 
ACATCCCATC TTAGGTCATC TTCTGTAGAA GATGTTAAAC TAATTATAAG CGAGGGGAGA 
CCTACCATAG AAGTTCGAAG ATGTAGCATG CCTTCTGTCA TTTGTGAACA TACCAAACAA 
TTCCAAACAA TATCAGAAGA GAGCAATCAA GGTAGCTTAT TAACTGTGCC AGGAGATACT 
AGTCCTTCTC CCAAACCTGA GGTATTCTCA AATGTGCCTG AAAGAGACCT TTCAAATGTA 
TCTAACATAC ATTCCAGTTT TGCAACTTCT CCAACTGGAG CTTCAAACAG CAAGTATGTT 
TCAGCTGATA GAAATCTCAT CAAGAATACT GCCCCAGTGA ACACTGTAAT GGACAGTCCA 
GTGCATTTAG AGCCATCTAG TCAGGTTGGT GTGATCCAGA ATAAATCATG GGAGATGCCT 
GTTGATAGAC TAGAGACATT AAGCACCAGA GACTTTATCT GCCCAAATTC TAACATACCT 
GATCAAGAAT CCTCTCTTCA GAGTTTTTGT AATTCTGAAA ATAAGGTATT GAAAGAAAAT 
GCTGATTTTT TATCCCTGCG CCAGACTGAA CTGCCAGGAA ACTCTTGTGC TCAGGATCCG 
GCATCCTTTA TGCCTCCACA GCAGCCTTGC TCTTTCCCCA GCCAATCACT TTCAGATGCT 
GAATCGATTT CTAAACATAT GTCTTTGTCA TATGTTGCTA ATCAAGAGCC AGGTATTTTA 
CAACAAAAAA ATGCAGTTCA GATTATTAGT TCTGCTTTAG ATACTGATAA TGAATCTACA 
AAAGATACAG AAAATACTTT TGTCCTAGGA GATGTTCAAA AAACAGATGC CTTTGTCCCA 
GTGTACTCTG ACAGCACTAT TCAAGAAGCA TCACCAAACT TTGAGAAAGC TTATACTTTA 
CCTGTGTTAC CATCAGAAAA GGACTTTAAT GGAAGTGATG CCTCTACCCA GCTAAATACA 
CATTATGCAT TTAGCAAACT AACTTACAAG TCTTCCAGTG GCCATGAAGT TGAGAATAGC 
ACAACTGATA CTCAGGTCAT TTCACATGAA AAAGAAAACA AACTGGAGAG TTTGGTTTTA 
ACTCATTTGA GTAGGTGTGA TTCTGATTTA TGTGAAATGA ATGCAGGGAT GCCAAAAGGA 
AACCTAAATG AACAAGATCC AAAACATTGT CCTGAAAGTG AAAAGTGTTT GCTTTCCATA 
GAAGATGAGG AATCTCAACA AAGCATTTTA TCAAGTCTGG AAAACCATTC ACAGCAGTCA 
ACTCAACCAG AAATGCATAA ATATGGTCAG TTAGTTAAAG TAGAATTAGA AGAAAATGCC 
GAAGATGATA AAACTGAAAA CCAAATCCCT CAAAGAATGA CTAGAAACAA AGCAAATACA 
ATGGCAAATC AAAGCAAACA GATTCTTGCT AGCTGTACAC TATTATCAGA AAAAGACAGT 
GAATCCTCAT CTCCTAGAGG AAGAATAAGA TTAACTGAAG ATGACGATCC TCAAATTCAC 
CATCCACGGA AAAGGAAAGT GTCACGTGTA CCTCAGCCTG TGCAAGTGAG TCCCTCTTTA 
CTACAAGCAA AAGAGAAAAC TCAGCAATCT CTGGCAGCCA TTGTAGATTC TCTAAAACTA 
GATGAGATTC AGCCATACAG TTCAGAGAGA GCAAATCCAT ATTTTGAATA CTTGCACATA 
AGGAAAAAAA TAGAAGAAAA ACGCAAATTA CTGTGTAGTG TGATTCCTCA AGCACCTCAG 
TACTATGACG AATATGTAAC ATTTAACGGA TCATATCTCC TGGATGGAAA CCCCTTAAGC 
AAGATTTGTA TTCCCACAAT TACACCACCA CCTTCACTGT CAGATCCACT TAAAGAGCTT 
TTTCGACAAC AGGAAGTTGT AAGGATGAAA CTACGTTTGC AACACAGTAT TGAAAGGGAA 
AAACTCATTG TATCCAACGA ACAAGAAGTT TTGCGAGTTC ATTACAGAGC TGCAAGAACA 
TTGGCAAATC AAACACTGCC ATTTAGTGCT TGTACTGTTT TGCTGGATGC CGAAGTATAC 
AATGTACCAT TGGACTCTCA GTCTGATGAC AGTAAAACTT CTGTGAGGGA TCGCTTTAAT 
GCAAGACAAT TCATGTCTTG GTTACAAGAT GTGGATGATA AATTTGACAA ATTAAAGACC 
TGTCTTTTAA TGAGGCAACA ACATGAAGCT GCGGCTTTAA ATGCTGTCCA GAGGTTAGAA 
TGGCAGCTCA AACTCCAGGA ACTTGATCCT GCCACCTATA AATCTATCAG CATTTACGAA 
ATCCAGGAGT TTTATGTTCC CCTTGTTGAT GTTAACGACG ACTTTGAATT GACTCCTATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_005258095.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258152.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X3, mRNA.

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CloneID	OHu50996
Clone ID Related Accession (Same CDS sequence)	XM_005258095.3 , XM_017025662.1 , XM_017025661.1 , XM_017025661.2 , XM_017025662.2 , XM_005258095.2
Accession Version	XM_005258095.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005258095.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAATTCC ACACCAAATC ATCCATCACA AACAACGCCT 
GCCCAAAAGA AAACTCCCAG TTCTTCATCT CGACAGAAAG ATAAAGTTAA TAAAAGAAAT 
GAACGTGGTG AAACTCCTTT ACACATGGCT GCTATTCGAG GAGATGTGAA ACAAGTTAAA 
GAATTAATAA GTTTAGGGGC AAATGTGAAT GTGAAAGATT TTGCAGGTTG GACACCACTG 
CATGAAGCTT GCAATGTTGG ATATTACGAT GTTGCTAAGA TACTTATAGC AGCTGGAGCA 
GATGTTAACA CACAAGGATT AGATGATGAC ACTCCACTCC ATGATTCTGC TAGTAGTGGG 
CACAGAGATA TAGTAAAGCT GTTACTTCGT CACGGTGGAA ATCCATTTCA AGCTAATAAA 
CATGGGGAGC GTCCAGTGGA TGTAGCAGAA ACAGAGGAGT TGGAGTTGCT ACTAAAAAGA 
GAGGTGCCTT TATCTGATGA TGATGAAAGT TACACAGATT CCGAAGAGGC TCAATCTGTA 
AATCCTTCTA GTGTTGATGA AAATATTGAC TCTGAAACAG AGAAAGACTC TCTCATCTGT 
GAAAGTAAAC AGATACTTCC CAGTAAAACA CCTCTTCCAT CTGCCCTTGA TGAGTATGAG 
TTCAAAGATG ATGATGATGA AGAAATTAAT AAGATGATTG ATGATAGGCA TATTCTTAGG 
AAAGAACAAC GAAAAGAAAA TGAACCTGAA GCAGAAAAAA CTCATTTATT TGCAAAACAG 
GAGAAAGCCT TCTATCCTAA ATCATTTAAA AGTAAAAAAC AAAAGCCATC TAGGGTCTTA 
TATTCAAGTA CTGAAAGTTC TGATGAAGAA GCTCTTCAGA ATAAAAAGAT TTCTACTTCA 
TGTTCCGTCA TCCCTGAAAC ATCAAATTCT GATATGCAAA CCAAAAAGGA ATATGTAGTT 
TCAGGTGAAC ACAAACAGAA AGGCAAAGTT AAAAGAAAAT TGAAAAATCA GAATAAAAAT 
AAAGAGAACC AAGAGCTAAA GCAAGAAAAG GAAGGAAAAG AAAATACAAG AATAACAAAC 
TTGACAGTAA ATACTGGACT AGATTGTTCA GAAAAGACCA GAGAGGAGGG GAACTTTAGG 
AAATCTTTTA GCCCAAAAGA TGATACTTCA TTACATTTAT TTCATATTTC CACTGGTAAA 
TCTCCCAAAC ATTCTTGTGG ATTAAGTGAA AAACAGTCAA CACCACTAAA ACAAGAACAT 
ACTAAAACAT GTTTATCACC AGGAAGTTCT GAAATGTCAT TACAGCCTGA TCTTGTTCGG 
TATGATAATA CAGAATCTGA ATTCTTGCCA GAAAGTTCAA GTGTAAAATC TTGTAAGCAT 
AAGGAAAAAA GCAAACATCA GAAAGATTTC CACTTAGAAT TTGGTGAAAA ATCAAATGCC 
AAAATAAAGG ATGAAGATCA TAGTCCAACA TTTGAAAATT CAGATTGCAC ACTGAAAAAA 
ATGGATAAAG AAGGTAAAAC ATTAAAAAAA CATAAATTGA AGCATAAAGA GAGGGAAAAA 
GAAAAGCATA AAAAAGAAAT TGAAGGTGAA AAGGAAAAAT ACAAAACTAA GGATAGTGCC 
AAAGAACTGC AGAGGAGTGT GGAATTTGAT AGAGAATTTT GGAAAGAGAA TTTTTTTAAA 
AGTGATGAAA CTGAAGATCT CTTTTTAAAT ATGGAACATG AATCCTTAAC ATTAGAAAAA 
AAATCAAAAT TGGAAAAAAA CATCAAAGAT GATAAATCAA CCAAGGAAAA GCATGTGTCA 
AAAGAGAGGA ACTTTAAAGA GGAACGAGAC AAGATTAAAA AGGAAAGCGA GAAATCTTTT 
AGGGAGGAAA AAATAAAAGA TCTAAAAGAA GAGAGAGAAA ACATACCCAC AGATAAAGAC 
TCAGAATTTA CTTCTTTGGG TATGAGTGCC ATTGAGGAAT CTATAGGGCT TCATTTAGTG 
GAAAAGGAAA TAGACATTGA AAAACAAGAA AAGCATATAA AGGAAAGTAA AGAAAAACCT 
GAGAAGCGAT CTCAAATTAA AGAAAAGGAC ATTGAGAAGA TGGAAAGAAA AACCTTTGAA 
AAAGAAAAGA AGATAAAACA TGAGCATAAG TCAGAAAAAG ACAAATTAGA TCTTAGTGAA 
TGTGTTGATA AAATAAAAGA AAAGGACAAG CTATATTCGC ATCACACAGA AAAATGCCAT 
AAAGAAGGTG AGAAGAGCAA AAATACTGCT GCTATTAAAA AAACTGACGA CAGAGAGAAA 
AGTAGAGAAA AGATGGATAG GAAACATGAC AAAGAAAAGC CTGAAAAAGA GAGGCATCTA 
GCAGAAAGCA AAGAAAAGCA CTTGATGGAG AAAAAAAATA AACAATCAGA TAATAGTGAA 
TACAGTAAAT CAGAAAAAGG CAAAAATAAA GAAAAAGACA GGGAGCTAGA TAAAAAGGAA 
AAATCTAGAG ATAAAGAAAG TATAAATATA ACTAACTCCA AACACATACA GGAAGAAAAA 
AAATCAAGTA TAGTAGACGG TAATAAAGCA CAACATGAAA AACCCTTATC CCTTAAAGAA 
AAAACAAAAG ATGAACCTTT GAAAACTCCA GATGGAAAAG AAAAAGATAA AAAAGATAAA 
GATATAGATA GATACAAAGA ACGAGACAAA CATAAAGATA AAATTCAAAT AAATAGCTTA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCTAAGT CATCACCAGC ATCAAAAGAT 
ACCCGACCTA AAGAAAAGAG GTTAGTGAAT GATGATTTAA TGCAGACAAG TTTTGAACGA 
ATGCTAAGCC TTAAAGACCT AGAAATAGAA CAGTGGCACA AAAAACATAA GGAAAAAATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAC CGAAACTGTT TAGAACTTAA AATAAAAGAT 
AAAGAAAAAA CAAAGCATAC ACCAACTGAA TCCAAAAATA AAGAACTTAC TAGGTCAAAG 
AGTTCAGAAG TGACTGATGC ATATACCAAG GAGAAACAAC CTAAAGATGC TGTAAGTAAC 
AGATCACAAT CTGTTGACAC CAAAAATGTA ATGACTTTAG GGAAGTCATC TTTTGTTTCA 
GATAATAGCT TAAACAGGTC TCCTAGATCA GAAAATGAAA AGCCGGGTCT CAGCTCCAGA 
TCTGTATCCA TGATTTCTGT TGCTAGTTCA GAAGATTCCT GCCATACTAC AGTGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCTGAC TTTATGTTAG AGAGTTCAGA ATCCCAAATG 
TCCTTTTCCC AGTCACCTTT TTTGTCAATT GCCAAATCTC CTGCTCTTCA TGAAAGGGAA 
TTGGACAGCC TGGCTGACTT GCCGGAGCGG ATTAAACCAC CATATGCAAA CAGACTTTCA 
ACATCCCATC TTAGGTCATC TTCTGTAGAA GATGTTAAAC TAATTATAAG CGAGGGGAGA 
CCTACCATAG AAGTTCGAAG ATGTAGCATG CCTTCTGTCA TTTGTGAACA TACCAAACAA 
TTCCAAACAA TATCAGAAGA GAGCAATCAA GGTAGCTTAT TAACTGTGCC AGGAGATACT 
AGTCCTTCTC CCAAACCTGA GGTATTCTCA AATGTGCCTG AAAGAGACCT TTCAAATGTA 
TCTAACATAC ATTCCAGTTT TGCAACTTCT CCAACTGGAG CTTCAAACAG CAAGTATGTT 
TCAGCTGATA GAAATCTCAT CAAGAATACT GCCCCAGTGA ACACTGTAAT GGACAGTCCA 
GTGCATTTAG AGCCATCTAG TCAGGTTGGT GTGATCCAGA ATAAATCATG GGAGATGCCT 
GTTGATAGAC TAGAGACATT AAGCACCAGA GACTTTATCT GCCCAAATTC TAACATACCT 
GATCAAGAAT CCTCTCTTCA GAGTTTTTGT AATTCTGAAA ATAAGGTATT GAAAGAAAAT 
GCTGATTTTT TATCCCTGCG CCAGACTGAA CTGCCAGGAA ACTCTTGTGC TCAGGATCCG 
GCATCCTTTA TGCCTCCACA GCAGCCTTGC TCTTTCCCCA GCCAATCACT TTCAGATGCT 
GAATCGATTT CTAAACATAT GTCTTTGTCA TATGTTGCTA ATCAAGAGCC AGGTATTTTA 
CAACAAAAAA ATGCAGTTCA GATTATTAGT TCTGCTTTAG ATACTGATAA TGAATCTACA 
AAAGATACAG AAAATACTTT TGTCCTAGGA GATGTTCAAA AAACAGATGC CTTTGTCCCA 
GTGTACTCTG ACAGCACTAT TCAAGAAGCA TCACCAAACT TTGAGAAAGC TTATACTTTA 
CCTGTGTTAC CATCAGAAAA GGACTTTAAT GGAAGTGATG CCTCTACCCA GCTAAATACA 
CATTATGCAT TTAGCAAACT AACTTACAAG TCTTCCAGTG GCCATGAAGT TGAGAATAGC 
ACAACTGATA CTCAGGTCAT TTCACATGAA AAAGAAAACA AACTGGAGAG TTTGGTTTTA 
ACTCATTTGA GTAGGTGTGA TTCTGATTTA TGTGAAATGA ATGCAGGGAT GCCAAAAGGA 
AACCTAAATG AACAAGATCC AAAACATTGT CCTGAAAGTG AAAAGTGTTT GCTTTCCATA 
GAAGATGAGG AATCTCAACA AAGCATTTTA TCAAGTCTGG AAAACCATTC ACAGCAGTCA 
ACTCAACCAG AAATGCATAA ATATGGTCAG TTAGTTAAAG TAGAATTAGA AGAAAATGCC 
GAAGATGATA AAACTGAAAA CCAAATCCCT CAAAGAATGA CTAGAAACAA AGCAAATACA 
ATGGCAAATC AAAGCAAACA GATTCTTGCT AGCTGTACAC TATTATCAGA AAAAGACAGT 
GAATCCTCAT CTCCTAGAGG AAGAATAAGA TTAACTGAAG ATGACGATCC TCAAATTCAC 
CATCCACGGA AAAGGAAAGT GTCACGTGTA CCTCAGCCTG TGCAAGTGAG TCCCTCTTTA 
CTACAAGCAA AAGAGAAAAC TCAGCAATCT CTGGCAGCCA TTGTAGATTC TCTAAAACTA 
GATGAGATTC AGCCATACAG TTCAGAGAGA GCAAATCCAT ATTTTGAATA CTTGCACATA 
AGGAAAAAAA TAGAAGAAAA ACGCAAATTA CTGTGTAGTG TGATTCCTCA AGCACCTCAG 
TACTATGACG AATATGTAAC ATTTAACGGA TCATATCTCC TGGATGGAAA CCCCTTAAGC 
AAGATTTGTA TTCCCACAAT TACACCACCA CCTTCACTGT CAGATCCACT TAAAGAGCTT 
TTTCGACAAC AGGAAGTTGT AAGGATGAAA CTACGTTTGC AACACAGTAT TGAAAGGGAA 
AAACTCATTG TATCCAACGA ACAAGAAGTT TTGCGAGTTC ATTACAGAGC TGCAAGAACA 
TTGGCAAATC AAACACTGCC ATTTAGTGCT TGTACTGTTT TGCTGGATGC CGAAGTATAC 
AATGTACCAT TGGACTCTCA GTCTGATGAC AGTAAAACTT CTGTGAGGGA TCGCTTTAAT 
GCAAGACAAT TCATGTCTTG GTTACAAGAT GTGGATGATA AATTTGACAA ATTAAAGACC 
TGTCTTTTAA TGAGGCAACA ACATGAAGCT GCGGCTTTAA ATGCTGTCCA GAGGTTAGAA 
TGGCAGCTCA AACTCCAGGA ACTTGATCCT GCCACCTATA AATCTATCAG CATTTACGAA 
ATCCAGGAGT TTTATGTTCC CCTTGTTGAT GTTAACGACG ACTTTGAATT GACTCCTATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258152.1
CDS	237..6419
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNNSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_005258095.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005258095.2

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAATTCC ACACCAAATC ATCCATCACA AACAACGCCT 
GCCCAAAAGA AAACTCCCAG TTCTTCATCT CGACAGAAAG ATAAAGTTAA TAAAAGAAAT 
GAACGTGGTG AAACTCCTTT ACACATGGCT GCTATTCGAG GAGATGTGAA ACAAGTTAAA 
GAATTAATAA GTTTAGGGGC AAATGTGAAT GTGAAAGATT TTGCAGGTTG GACACCACTG 
CATGAAGCTT GCAATGTTGG ATATTACGAT GTTGCTAAGA TACTTATAGC AGCTGGAGCA 
GATGTTAACA CACAAGGATT AGATGATGAC ACTCCACTCC ATGATTCTGC TAGTAGTGGG 
CACAGAGATA TAGTAAAGCT GTTACTTCGT CACGGTGGAA ATCCATTTCA AGCTAATAAA 
CATGGGGAGC GTCCAGTGGA TGTAGCAGAA ACAGAGGAGT TGGAGTTGCT ACTAAAAAGA 
GAGGTGCCTT TATCTGATGA TGATGAAAGT TACACAGATT CCGAAGAGGC TCAATCTGTA 
AATCCTTCTA GTGTTGATGA AAATATTGAC TCTGAAACAG AGAAAGACTC TCTCATCTGT 
GAAAGTAAAC AGATACTTCC CAGTAAAACA CCTCTTCCAT CTGCCCTTGA TGAGTATGAG 
TTCAAAGATG ATGATGATGA AGAAATTAAT AAGATGATTG ATGATAGGCA TATTCTTAGG 
AAAGAACAAC GAAAAGAAAA TGAACCTGAA GCAGAAAAAA CTCATTTATT TGCAAAACAG 
GAGAAAGCCT TCTATCCTAA ATCATTTAAA AGTAAAAAAC AAAAGCCATC TAGGGTCTTA 
TATTCAAGTA CTGAAAGTTC TGATGAAGAA GCTCTTCAGA ATAAAAAGAT TTCTACTTCA 
TGTTCCGTCA TCCCTGAAAC ATCAAATTCT GATATGCAAA CCAAAAAGGA ATATGTAGTT 
TCAGGTGAAC ACAAACAGAA AGGCAAAGTT AAAAGAAAAT TGAAAAATCA GAATAAAAAT 
AAAGAGAACC AAGAGCTAAA GCAAGAAAAG GAAGGAAAAG AAAATACAAG AATAACAAAC 
TTGACAGTAA ATACTGGACT AGATTGTTCA GAAAAGACCA GAGAGGAGGG GAACTTTAGG 
AAATCTTTTA GCCCAAAAGA TGATACTTCA TTACATTTAT TTCATATTTC CACTGGTAAA 
TCTCCCAAAC ATTCTTGTGG ATTAAGTGAA AAACAGTCAA CACCACTAAA ACAAGAACAT 
ACTAAAACAT GTTTATCACC AGGAAGTTCT GAAATGTCAT TACAGCCTGA TCTTGTTCGG 
TATGATAATA CAGAATCTGA ATTCTTGCCA GAAAGTTCAA GTGTAAAATC TTGTAAGCAT 
AAGGAAAAAA GCAAACATCA GAAAGATTTC CACTTAGAAT TTGGTGAAAA ATCAAATGCC 
AAAATAAAGG ATGAAGATCA TAGTCCAACA TTTGAAAATT CAGATTGCAC ACTGAAAAAA 
ATGGATAAAG AAGGTAAAAC ATTAAAAAAA CATAAATTGA AGCATAAAGA GAGGGAAAAA 
GAAAAGCATA AAAAAGAAAT TGAAGGTGAA AAGGAAAAAT ACAAAACTAA GGATAGTGCC 
AAAGAACTGC AGAGGAGTGT GGAATTTGAT AGAGAATTTT GGAAAGAGAA TTTTTTTAAA 
AGTGATGAAA CTGAAGATCT CTTTTTAAAT ATGGAACATG AATCCTTAAC ATTAGAAAAA 
AAATCAAAAT TGGAAAAAAA CATCAAAGAT GATAAATCAA CCAAGGAAAA GCATGTGTCA 
AAAGAGAGGA ACTTTAAAGA GGAACGAGAC AAGATTAAAA AGGAAAGCGA GAAATCTTTT 
AGGGAGGAAA AAATAAAAGA TCTAAAAGAA GAGAGAGAAA ACATACCCAC AGATAAAGAC 
TCAGAATTTA CTTCTTTGGG TATGAGTGCC ATTGAGGAAT CTATAGGGCT TCATTTAGTG 
GAAAAGGAAA TAGACATTGA AAAACAAGAA AAGCATATAA AGGAAAGTAA AGAAAAACCT 
GAGAAGCGAT CTCAAATTAA AGAAAAGGAC ATTGAGAAGA TGGAAAGAAA AACCTTTGAA 
AAAGAAAAGA AGATAAAACA TGAGCATAAG TCAGAAAAAG ACAAATTAGA TCTTAGTGAA 
TGTGTTGATA AAATAAAAGA AAAGGACAAG CTATATTCGC ATCACACAGA AAAATGCCAT 
AAAGAAGGTG AGAAGAGCAA AAATACTGCT GCTATTAAAA AAACTGACGA CAGAGAGAAA 
AGTAGAGAAA AGATGGATAG GAAACATGAC AAAGAAAAGC CTGAAAAAGA GAGGCATCTA 
GCAGAAAGCA AAGAAAAGCA CTTGATGGAG AAAAAAAATA AACAATCAGA TAATAGTGAA 
TACAGTAAAT CAGAAAAAGG CAAAAATAAA GAAAAAGACA GGGAGCTAGA TAAAAAGGAA 
AAATCTAGAG ATAAAGAAAG TATAAATATA ACTAACTCCA AACACATACA GGAAGAAAAA 
AAATCAAGTA TAGTAGACGG TAATAAAGCA CAACATGAAA AACCCTTATC CCTTAAAGAA 
AAAACAAAAG ATGAACCTTT GAAAACTCCA GATGGAAAAG AAAAAGATAA AAAAGATAAA 
GATATAGATA GATACAAAGA ACGAGACAAA CATAAAGATA AAATTCAAAT AAATAGCTTA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCTAAGT CATCACCAGC ATCAAAAGAT 
ACCCGACCTA AAGAAAAGAG GTTAGTGAAT GATGATTTAA TGCAGACAAG TTTTGAACGA 
ATGCTAAGCC TTAAAGACCT AGAAATAGAA CAGTGGCACA AAAAACATAA GGAAAAAATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAC CGAAACTGTT TAGAACTTAA AATAAAAGAT 
AAAGAAAAAA CAAAGCATAC ACCAACTGAA TCCAAAAATA AAGAACTTAC TAGGTCAAAG 
AGTTCAGAAG TGACTGATGC ATATACCAAG GAGAAACAAC CTAAAGATGC TGTAAGTAAC 
AGATCACAAT CTGTTGACAC CAAAAATGTA ATGACTTTAG GGAAGTCATC TTTTGTTTCA 
GATAATAGCT TAAACAGGTC TCCTAGATCA GAAAATGAAA AGCCGGGTCT CAGCTCCAGA 
TCTGTATCCA TGATTTCTGT TGCTAGTTCA GAAGATTCCT GCCATACTAC AGTGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCTGAC TTTATGTTAG AGAGTTCAGA ATCCCAAATG 
TCCTTTTCCC AGTCACCTTT TTTGTCAATT GCCAAATCTC CTGCTCTTCA TGAAAGGGAA 
TTGGACAGCC TGGCTGACTT GCCGGAGCGG ATTAAACCAC CATATGCAAA CAGACTTTCA 
ACATCCCATC TTAGGTCATC TTCTGTAGAA GATGTTAAAC TAATTATAAG CGAGGGGAGA 
CCTACCATAG AAGTTCGAAG ATGTAGCATG CCTTCTGTCA TTTGTGAACA TACCAAACAA 
TTCCAAACAA TATCAGAAGA GAGCAATCAA GGTAGCTTAT TAACTGTGCC AGGAGATACT 
AGTCCTTCTC CCAAACCTGA GGTATTCTCA AATGTGCCTG AAAGAGACCT TTCAAATGTA 
TCTAACATAC ATTCCAGTTT TGCAACTTCT CCAACTGGAG CTTCAAACAG CAAGTATGTT 
TCAGCTGATA GAAATCTCAT CAAGAATACT GCCCCAGTGA ACACTGTAAT GGACAGTCCA 
GTGCATTTAG AGCCATCTAG TCAGGTTGGT GTGATCCAGA ATAAATCATG GGAGATGCCT 
GTTGATAGAC TAGAGACATT AAGCACCAGA GACTTTATCT GCCCAAATTC TAACATACCT 
GATCAAGAAT CCTCTCTTCA GAGTTTTTGT AATTCTGAAA ATAAGGTATT GAAAGAAAAT 
GCTGATTTTT TATCCCTGCG CCAGACTGAA CTGCCAGGAA ACTCTTGTGC TCAGGATCCG 
GCATCCTTTA TGCCTCCACA GCAGCCTTGC TCTTTCCCCA GCCAATCACT TTCAGATGCT 
GAATCGATTT CTAAACATAT GTCTTTGTCA TATGTTGCTA ATCAAGAGCC AGGTATTTTA 
CAACAAAAAA ATGCAGTTCA GATTATTAGT TCTGCTTTAG ATACTGATAA TGAATCTACA 
AAAGATACAG AAAATACTTT TGTCCTAGGA GATGTTCAAA AAACAGATGC CTTTGTCCCA 
GTGTACTCTG ACAGCACTAT TCAAGAAGCA TCACCAAACT TTGAGAAAGC TTATACTTTA 
CCTGTGTTAC CATCAGAAAA GGACTTTAAT GGAAGTGATG CCTCTACCCA GCTAAATACA 
CATTATGCAT TTAGCAAACT AACTTACAAG TCTTCCAGTG GCCATGAAGT TGAGAATAGC 
ACAACTGATA CTCAGGTCAT TTCACATGAA AAAGAAAACA AACTGGAGAG TTTGGTTTTA 
ACTCATTTGA GTAGGTGTGA TTCTGATTTA TGTGAAATGA ATGCAGGGAT GCCAAAAGGA 
AACCTAAATG AACAAGATCC AAAACATTGT CCTGAAAGTG AAAAGTGTTT GCTTTCCATA 
GAAGATGAGG AATCTCAACA AAGCATTTTA TCAAGTCTGG AAAACCATTC ACAGCAGTCA 
ACTCAACCAG AAATGCATAA ATATGGTCAG TTAGTTAAAG TAGAATTAGA AGAAAATGCC 
GAAGATGATA AAACTGAAAA CCAAATCCCT CAAAGAATGA CTAGAAACAA AGCAAATACA 
ATGGCAAATC AAAGCAAACA GATTCTTGCT AGCTGTACAC TATTATCAGA AAAAGACAGT 
GAATCCTCAT CTCCTAGAGG AAGAATAAGA TTAACTGAAG ATGACGATCC TCAAATTCAC 
CATCCACGGA AAAGGAAAGT GTCACGTGTA CCTCAGCCTG TGCAAGTGAG TCCCTCTTTA 
CTACAAGCAA AAGAGAAAAC TCAGCAATCT CTGGCAGCCA TTGTAGATTC TCTAAAACTA 
GATGAGATTC AGCCATACAG TTCAGAGAGA GCAAATCCAT ATTTTGAATA CTTGCACATA 
AGGAAAAAAA TAGAAGAAAA ACGCAAATTA CTGTGTAGTG TGATTCCTCA AGCACCTCAG 
TACTATGACG AATATGTAAC ATTTAACGGA TCATATCTCC TGGATGGAAA CCCCTTAAGC 
AAGATTTGTA TTCCCACAAT TACACCACCA CCTTCACTGT CAGATCCACT TAAAGAGCTT 
TTTCGACAAC AGGAAGTTGT AAGGATGAAA CTACGTTTGC AACACAGTAT TGAAAGGGAA 
AAACTCATTG TATCCAACGA ACAAGAAGTT TTGCGAGTTC ATTACAGAGC TGCAAGAACA 
TTGGCAAATC AAACACTGCC ATTTAGTGCT TGTACTGTTT TGCTGGATGC CGAAGTATAC 
AATGTACCAT TGGACTCTCA GTCTGATGAC AGTAAAACTT CTGTGAGGGA TCGCTTTAAT 
GCAAGACAAT TCATGTCTTG GTTACAAGAT GTGGATGATA AATTTGACAA ATTAAAGACC 
TGTCTTTTAA TGAGGCAACA ACATGAAGCT GCGGCTTTAA ATGCTGTCCA GAGGTTAGAA 
TGGCAGCTCA AACTCCAGGA ACTTGATCCT GCCACCTATA AATCTATCAG CATTTACGAA 
ATCCAGGAGT TTTATGTTCC CCTTGTTGAT GTTAACGACG ACTTTGAATT GACTCCTATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025666.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881155.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X10, mRNA.

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CloneID	OHu50997
Clone ID Related Accession (Same CDS sequence)	XM_017025666.1 , XM_017025664.2 , XM_017025665.2 , XM_017025666.2 , XM_017025664.1 , XM_005258096.3 , XM_005258096.2 , XM_017025665.1
Accession Version	XM_017025666.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAATTC CACACCAAAT CATCCATCAC AAACAACGCC TGCCCAAAAG 
AAAACTCCCA GTTCTTCATC TCGACAGAAA GATAAAGTTA ATAAAAGAAA TGAACGTGGT 
GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA AACAAGTTAA AGAATTAATA 
AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT GGACACCACT GCATGAAGCT 
TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG CAGCTGGAGC AGATGTTAAC 
ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG CTAGTAGTGG GCACAGAGAT 
ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC AAGCTAATAA ACATGGGGAG 
CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC TACTAAAAAG AGAGGTGCCT 
TTATCTGATG ATGATGAAAG TTACACAGAT TCCGAAGAGG CTCAATCTGT AAATCCTTCT 
AGTGTTGATG AAAATATTGA CTCTGAAACA GAGAAAGACT CTCTCATCTG TGAAAGTAAA 
CAGATACTTC CCAGTAAAAC ACCTCTTCCA TCTGCCCTTG ATGAGTATGA GTTCAAAGAT 
GATGATGATG AAGAAATTAA TAAGATGATT GATGATAGGC ATATTCTTAG GAAAGAACAA 
CGAAAAGAAA ATGAACCTGA AGCAGAAAAA ACTCATTTAT TTGCAAAACA GGAGAAAGCC 
TTCTATCCTA AATCATTTAA AAGTAAAAAA CAAAAGCCAT CTAGGGTCTT ATATTCAAGT 
ACTGAAAGTT CTGATGAAGA AGCTCTTCAG AATAAAAAGA TTTCTACTTC ATGTTCCGTC 
ATCCCTGAAA CATCAAATTC TGATATGCAA ACCAAAAAGG AATATGTAGT TTCAGGTGAA 
CACAAACAGA AAGGCAAAGT TAAAAGAAAA TTGAAAAATC AGAATAAAAA TAAAGAGAAC 
CAAGAGCTAA AGCAAGAAAA GGAAGGAAAA GAAAATACAA GAATAACAAA CTTGACAGTA 
AATACTGGAC TAGATTGTTC AGAAAAGACC AGAGAGGAGG GGAACTTTAG GAAATCTTTT 
AGCCCAAAAG ATGATACTTC ATTACATTTA TTTCATATTT CCACTGGTAA ATCTCCCAAA 
CATTCTTGTG GATTAAGTGA AAAACAGTCA ACACCACTAA AACAAGAACA TACTAAAACA 
TGTTTATCAC CAGGAAGTTC TGAAATGTCA TTACAGCCTG ATCTTGTTCG GTATGATAAT 
ACAGAATCTG AATTCTTGCC AGAAAGTTCA AGTGTAAAAT CTTGTAAGCA TAAGGAAAAA 
AGCAAACATC AGAAAGATTT CCACTTAGAA TTTGGTGAAA AATCAAATGC CAAAATAAAG 
GATGAAGATC ATAGTCCAAC ATTTGAAAAT TCAGATTGCA CACTGAAAAA AATGGATAAA 
GAAGGTAAAA CATTAAAAAA ACATAAATTG AAGCATAAAG AGAGGGAAAA AGAAAAGCAT 
AAAAAAGAAA TTGAAGGTGA AAAGGAAAAA TACAAAACTA AGGATAGTGC CAAAGAACTG 
CAGAGGAGTG TGGAATTTGA TAGAGAATTT TGGAAAGAGA ATTTTTTTAA AAGTGATGAA 
ACTGAAGATC TCTTTTTAAA TATGGAACAT GAATCCTTAA CATTAGAAAA AAAATCAAAA 
TTGGAAAAAA ACATCAAAGA TGATAAATCA ACCAAGGAAA AGCATGTGTC AAAAGAGAGG 
AACTTTAAAG AGGAACGAGA CAAGATTAAA AAGGAAAGCG AGAAATCTTT TAGGGAGGAA 
AAAATAAAAG ATCTAAAAGA AGAGAGAGAA AACATACCCA CAGATAAAGA CTCAGAATTT 
ACTTCTTTGG GTATGAGTGC CATTGAGGAA TCTATAGGGC TTCATTTAGT GGAAAAGGAA 
ATAGACATTG AAAAACAAGA AAAGCATATA AAGGAAAGTA AAGAAAAACC TGAGAAGCGA 
TCTCAAATTA AAGAAAAGGA CATTGAGAAG ATGGAAAGAA AAACCTTTGA AAAAGAAAAG 
AAGATAAAAC ATGAGCATAA GTCAGAAAAA GACAAATTAG ATCTTAGTGA ATGTGTTGAT 
AAAATAAAAG AAAAGGACAA GCTATATTCG CATCACACAG AAAAATGCCA TAAAGAAGGT 
GAGAAGAGCA AAAATACTGC TGCTATTAAA AAAACTGACG ACAGAGAGAA AAGTAGAGAA 
AAGATGGATA GGAAACATGA CAAAGAAAAG CCTGAAAAAG AGAGGCATCT AGCAGAAAGC 
AAAGAAAAGC ACTTGATGGA GAAAAAAAAT AAACAATCAG ATAATAGTGA ATACAGTAAA 
TCAGAAAAAG GCAAAAATAA AGAAAAAGAC AGGGAGCTAG ATAAAAAGGA AAAATCTAGA 
GATAAAGAAA GTATAAATAT AACTAACTCC AAACACATAC AGGAAGAAAA AAAATCAAGT 
ATAGTAGACG GTAATAAAGC ACAACATGAA AAACCCTTAT CCCTTAAAGA AAAAACAAAA 
GATGAACCTT TGAAAACTCC AGATGGAAAA GAAAAAGATA AAAAAGATAA AGATATAGAT 
AGATACAAAG AACGAGACAA ACATAAAGAT AAAATTCAAA TAAATAGCTT ACTCAAACTA 
AAATCTGAAG CAGATAAGCC TAAACCTAAG TCATCACCAG CATCAAAAGA TACCCGACCT 
AAAGAAAAGA GGTTAGTGAA TGATGATTTA ATGCAGACAA GTTTTGAACG AATGCTAAGC 
CTTAAAGACC TAGAAATAGA ACAGTGGCAC AAAAAACATA AGGAAAAAAT TAAGCAAAAA 
GAAAAGGAAC GGTTGAGAAA CCGAAACTGT TTAGAACTTA AAATAAAAGA TAAAGAAAAA 
ACAAAGCATA CACCAACTGA ATCCAAAAAT AAAGAACTTA CTAGGTCAAA GAGTTCAGAA 
GTGACTGATG CATATACCAA GGAGAAACAA CCTAAAGATG CTGTAAGTAA CAGATCACAA 
TCTGTTGACA CCAAAAATGT AATGACTTTA GGGAAGTCAT CTTTTGTTTC AGATAATAGC 
TTAAACAGGT CTCCTAGATC AGAAAATGAA AAGCCGGGTC TCAGCTCCAG ATCTGTATCC 
ATGATTTCTG TTGCTAGTTC AGAAGATTCC TGCCATACTA CAGTGACAAC CCCAAGGCCT 
CCAGTTGAGT ATGACTCTGA CTTTATGTTA GAGAGTTCAG AATCCCAAAT GTCCTTTTCC 
CAGTCACCTT TTTTGTCAAT TGCCAAATCT CCTGCTCTTC ATGAAAGGGA ATTGGACAGC 
CTGGCTGACT TGCCGGAGCG GATTAAACCA CCATATGCAA ACAGACTTTC AACATCCCAT 
CTTAGGTCAT CTTCTGTAGA AGATGTTAAA CTAATTATAA GCGAGGGGAG ACCTACCATA 
GAAGTTCGAA GATGTAGCAT GCCTTCTGTC ATTTGTGAAC ATACCAAACA ATTCCAAACA 
ATATCAGAAG AGAGCAATCA AGGTAGCTTA TTAACTGTGC CAGGAGATAC TAGTCCTTCT 
CCCAAACCTG AGGTATTCTC AAATGTGCCT GAAAGAGACC TTTCAAATGT ATCTAACATA 
CATTCCAGTT TTGCAACTTC TCCAACTGGA GCTTCAAACA GCAAGTATGT TTCAGCTGAT 
AGAAATCTCA TCAAGAATAC TGCCCCAGTG AACACTGTAA TGGACAGTCC AGTGCATTTA 
GAGCCATCTA GTCAGGTTGG TGTGATCCAG AATAAATCAT GGGAGATGCC TGTTGATAGA 
CTAGAGACAT TAAGCACCAG AGACTTTATC TGCCCAAATT CTAACATACC TGATCAAGAA 
TCCTCTCTTC AGAGTTTTTG TAATTCTGAA AATAAGGTAT TGAAAGAAAA TGCTGATTTT 
TTATCCCTGC GCCAGACTGA ACTGCCAGGA AACTCTTGTG CTCAGGATCC GGCATCCTTT 
ATGCCTCCAC AGCAGCCTTG CTCTTTCCCC AGCCAATCAC TTTCAGATGC TGAATCGATT 
TCTAAACATA TGTCTTTGTC ATATGTTGCT AATCAAGAGC CAGGTATTTT ACAACAAAAA 
AATGCAGTTC AGATTATTAG TTCTGCTTTA GATACTGATA ATGAATCTAC AAAAGATACA 
GAAAATACTT TTGTCCTAGG AGATGTTCAA AAAACAGATG CCTTTGTCCC AGTGTACTCT 
GACAGCACTA TTCAAGAAGC ATCACCAAAC TTTGAGAAAG CTTATACTTT ACCTGTGTTA 
CCATCAGAAA AGGACTTTAA TGGAAGTGAT GCCTCTACCC AGCTAAATAC ACATTATGCA 
TTTAGCAAAC TAACTTACAA GTCTTCCAGT GGCCATGAAG TTGAGAATAG CACAACTGAT 
ACTCAGGTCA TTTCACATGA AAAAGAAAAC AAACTGGAGA GTTTGGTTTT AACTCATTTG 
AGTAGGTGTG ATTCTGATTT ATGTGAAATG AATGCAGGGA TGCCAAAAGG AAACCTAAAT 
GAACAAGATC CAAAACATTG TCCTGAAAGT GAAAAGTGTT TGCTTTCCAT AGAAGATGAG 
GAATCTCAAC AAAGCATTTT ATCAAGTCTG GAAAACCATT CACAGCAGTC AACTCAACCA 
GAAATGCATA AATATGGTCA GTTAGTTAAA GTAGAATTAG AAGAAAATGC CGAAGATGAT 
AAAACTGAAA ACCAAATCCC TCAAAGAATG ACTAGAAACA AAGCAAATAC AATGGCAAAT 
CAAAGCAAAC AGATTCTTGC TAGCTGTACA CTATTATCAG AAAAAGACAG TGAATCCTCA 
TCTCCTAGAG GAAGAATAAG ATTAACTGAA GATGACGATC CTCAAATTCA CCATCCACGG 
AAAAGGAAAG TGTCACGTGT ACCTCAGCCT GTGCAAGTGA GTCCCTCTTT ACTACAAGCA 
AAAGAGAAAA CTCAGCAATC TCTGGCAGCC ATTGTAGATT CTCTAAAACT AGATGAGATT 
CAGCCATACA GTTCAGAGAG AGCAAATCCA TATTTTGAAT ACTTGCACAT AAGGAAAAAA 
ATAGAAGAAA AACGCAAATT ACTGTGTAGT GTGATTCCTC AAGCACCTCA GTACTATGAC 
GAATATGTAA CATTTAACGG ATCATATCTC CTGGATGGAA ACCCCTTAAG CAAGATTTGT 
ATTCCCACAA TTACACCACC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TAAGGATGAA ACTACGTTTG CAACACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAACG AACAAGAAGT TTTGCGAGTT CATTACAGAG CTGCAAGAAC ATTGGCAAAT 
CAAACACTGC CATTTAGTGC TTGTACTGTT TTGCTGGATG CCGAAGTATA CAATGTACCA 
TTGGACTCTC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAA 
TTCATGTCTT GGTTACAAGA TGTGGATGAT AAATTTGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AACATGAAGC TGCGGCTTTA AATGCTGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATTTACGA AATCCAGGAG 
TTTTATGTTC CCCTTGTTGA TGTTAACGAC GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881155.1
CDS	323..6436
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNNSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025666.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025666.1

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAATTC CACACCAAAT CATCCATCAC AAACAACGCC TGCCCAAAAG 
AAAACTCCCA GTTCTTCATC TCGACAGAAA GATAAAGTTA ATAAAAGAAA TGAACGTGGT 
GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA AACAAGTTAA AGAATTAATA 
AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT GGACACCACT GCATGAAGCT 
TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG CAGCTGGAGC AGATGTTAAC 
ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG CTAGTAGTGG GCACAGAGAT 
ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC AAGCTAATAA ACATGGGGAG 
CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC TACTAAAAAG AGAGGTGCCT 
TTATCTGATG ATGATGAAAG TTACACAGAT TCCGAAGAGG CTCAATCTGT AAATCCTTCT 
AGTGTTGATG AAAATATTGA CTCTGAAACA GAGAAAGACT CTCTCATCTG TGAAAGTAAA 
CAGATACTTC CCAGTAAAAC ACCTCTTCCA TCTGCCCTTG ATGAGTATGA GTTCAAAGAT 
GATGATGATG AAGAAATTAA TAAGATGATT GATGATAGGC ATATTCTTAG GAAAGAACAA 
CGAAAAGAAA ATGAACCTGA AGCAGAAAAA ACTCATTTAT TTGCAAAACA GGAGAAAGCC 
TTCTATCCTA AATCATTTAA AAGTAAAAAA CAAAAGCCAT CTAGGGTCTT ATATTCAAGT 
ACTGAAAGTT CTGATGAAGA AGCTCTTCAG AATAAAAAGA TTTCTACTTC ATGTTCCGTC 
ATCCCTGAAA CATCAAATTC TGATATGCAA ACCAAAAAGG AATATGTAGT TTCAGGTGAA 
CACAAACAGA AAGGCAAAGT TAAAAGAAAA TTGAAAAATC AGAATAAAAA TAAAGAGAAC 
CAAGAGCTAA AGCAAGAAAA GGAAGGAAAA GAAAATACAA GAATAACAAA CTTGACAGTA 
AATACTGGAC TAGATTGTTC AGAAAAGACC AGAGAGGAGG GGAACTTTAG GAAATCTTTT 
AGCCCAAAAG ATGATACTTC ATTACATTTA TTTCATATTT CCACTGGTAA ATCTCCCAAA 
CATTCTTGTG GATTAAGTGA AAAACAGTCA ACACCACTAA AACAAGAACA TACTAAAACA 
TGTTTATCAC CAGGAAGTTC TGAAATGTCA TTACAGCCTG ATCTTGTTCG GTATGATAAT 
ACAGAATCTG AATTCTTGCC AGAAAGTTCA AGTGTAAAAT CTTGTAAGCA TAAGGAAAAA 
AGCAAACATC AGAAAGATTT CCACTTAGAA TTTGGTGAAA AATCAAATGC CAAAATAAAG 
GATGAAGATC ATAGTCCAAC ATTTGAAAAT TCAGATTGCA CACTGAAAAA AATGGATAAA 
GAAGGTAAAA CATTAAAAAA ACATAAATTG AAGCATAAAG AGAGGGAAAA AGAAAAGCAT 
AAAAAAGAAA TTGAAGGTGA AAAGGAAAAA TACAAAACTA AGGATAGTGC CAAAGAACTG 
CAGAGGAGTG TGGAATTTGA TAGAGAATTT TGGAAAGAGA ATTTTTTTAA AAGTGATGAA 
ACTGAAGATC TCTTTTTAAA TATGGAACAT GAATCCTTAA CATTAGAAAA AAAATCAAAA 
TTGGAAAAAA ACATCAAAGA TGATAAATCA ACCAAGGAAA AGCATGTGTC AAAAGAGAGG 
AACTTTAAAG AGGAACGAGA CAAGATTAAA AAGGAAAGCG AGAAATCTTT TAGGGAGGAA 
AAAATAAAAG ATCTAAAAGA AGAGAGAGAA AACATACCCA CAGATAAAGA CTCAGAATTT 
ACTTCTTTGG GTATGAGTGC CATTGAGGAA TCTATAGGGC TTCATTTAGT GGAAAAGGAA 
ATAGACATTG AAAAACAAGA AAAGCATATA AAGGAAAGTA AAGAAAAACC TGAGAAGCGA 
TCTCAAATTA AAGAAAAGGA CATTGAGAAG ATGGAAAGAA AAACCTTTGA AAAAGAAAAG 
AAGATAAAAC ATGAGCATAA GTCAGAAAAA GACAAATTAG ATCTTAGTGA ATGTGTTGAT 
AAAATAAAAG AAAAGGACAA GCTATATTCG CATCACACAG AAAAATGCCA TAAAGAAGGT 
GAGAAGAGCA AAAATACTGC TGCTATTAAA AAAACTGACG ACAGAGAGAA AAGTAGAGAA 
AAGATGGATA GGAAACATGA CAAAGAAAAG CCTGAAAAAG AGAGGCATCT AGCAGAAAGC 
AAAGAAAAGC ACTTGATGGA GAAAAAAAAT AAACAATCAG ATAATAGTGA ATACAGTAAA 
TCAGAAAAAG GCAAAAATAA AGAAAAAGAC AGGGAGCTAG ATAAAAAGGA AAAATCTAGA 
GATAAAGAAA GTATAAATAT AACTAACTCC AAACACATAC AGGAAGAAAA AAAATCAAGT 
ATAGTAGACG GTAATAAAGC ACAACATGAA AAACCCTTAT CCCTTAAAGA AAAAACAAAA 
GATGAACCTT TGAAAACTCC AGATGGAAAA GAAAAAGATA AAAAAGATAA AGATATAGAT 
AGATACAAAG AACGAGACAA ACATAAAGAT AAAATTCAAA TAAATAGCTT ACTCAAACTA 
AAATCTGAAG CAGATAAGCC TAAACCTAAG TCATCACCAG CATCAAAAGA TACCCGACCT 
AAAGAAAAGA GGTTAGTGAA TGATGATTTA ATGCAGACAA GTTTTGAACG AATGCTAAGC 
CTTAAAGACC TAGAAATAGA ACAGTGGCAC AAAAAACATA AGGAAAAAAT TAAGCAAAAA 
GAAAAGGAAC GGTTGAGAAA CCGAAACTGT TTAGAACTTA AAATAAAAGA TAAAGAAAAA 
ACAAAGCATA CACCAACTGA ATCCAAAAAT AAAGAACTTA CTAGGTCAAA GAGTTCAGAA 
GTGACTGATG CATATACCAA GGAGAAACAA CCTAAAGATG CTGTAAGTAA CAGATCACAA 
TCTGTTGACA CCAAAAATGT AATGACTTTA GGGAAGTCAT CTTTTGTTTC AGATAATAGC 
TTAAACAGGT CTCCTAGATC AGAAAATGAA AAGCCGGGTC TCAGCTCCAG ATCTGTATCC 
ATGATTTCTG TTGCTAGTTC AGAAGATTCC TGCCATACTA CAGTGACAAC CCCAAGGCCT 
CCAGTTGAGT ATGACTCTGA CTTTATGTTA GAGAGTTCAG AATCCCAAAT GTCCTTTTCC 
CAGTCACCTT TTTTGTCAAT TGCCAAATCT CCTGCTCTTC ATGAAAGGGA ATTGGACAGC 
CTGGCTGACT TGCCGGAGCG GATTAAACCA CCATATGCAA ACAGACTTTC AACATCCCAT 
CTTAGGTCAT CTTCTGTAGA AGATGTTAAA CTAATTATAA GCGAGGGGAG ACCTACCATA 
GAAGTTCGAA GATGTAGCAT GCCTTCTGTC ATTTGTGAAC ATACCAAACA ATTCCAAACA 
ATATCAGAAG AGAGCAATCA AGGTAGCTTA TTAACTGTGC CAGGAGATAC TAGTCCTTCT 
CCCAAACCTG AGGTATTCTC AAATGTGCCT GAAAGAGACC TTTCAAATGT ATCTAACATA 
CATTCCAGTT TTGCAACTTC TCCAACTGGA GCTTCAAACA GCAAGTATGT TTCAGCTGAT 
AGAAATCTCA TCAAGAATAC TGCCCCAGTG AACACTGTAA TGGACAGTCC AGTGCATTTA 
GAGCCATCTA GTCAGGTTGG TGTGATCCAG AATAAATCAT GGGAGATGCC TGTTGATAGA 
CTAGAGACAT TAAGCACCAG AGACTTTATC TGCCCAAATT CTAACATACC TGATCAAGAA 
TCCTCTCTTC AGAGTTTTTG TAATTCTGAA AATAAGGTAT TGAAAGAAAA TGCTGATTTT 
TTATCCCTGC GCCAGACTGA ACTGCCAGGA AACTCTTGTG CTCAGGATCC GGCATCCTTT 
ATGCCTCCAC AGCAGCCTTG CTCTTTCCCC AGCCAATCAC TTTCAGATGC TGAATCGATT 
TCTAAACATA TGTCTTTGTC ATATGTTGCT AATCAAGAGC CAGGTATTTT ACAACAAAAA 
AATGCAGTTC AGATTATTAG TTCTGCTTTA GATACTGATA ATGAATCTAC AAAAGATACA 
GAAAATACTT TTGTCCTAGG AGATGTTCAA AAAACAGATG CCTTTGTCCC AGTGTACTCT 
GACAGCACTA TTCAAGAAGC ATCACCAAAC TTTGAGAAAG CTTATACTTT ACCTGTGTTA 
CCATCAGAAA AGGACTTTAA TGGAAGTGAT GCCTCTACCC AGCTAAATAC ACATTATGCA 
TTTAGCAAAC TAACTTACAA GTCTTCCAGT GGCCATGAAG TTGAGAATAG CACAACTGAT 
ACTCAGGTCA TTTCACATGA AAAAGAAAAC AAACTGGAGA GTTTGGTTTT AACTCATTTG 
AGTAGGTGTG ATTCTGATTT ATGTGAAATG AATGCAGGGA TGCCAAAAGG AAACCTAAAT 
GAACAAGATC CAAAACATTG TCCTGAAAGT GAAAAGTGTT TGCTTTCCAT AGAAGATGAG 
GAATCTCAAC AAAGCATTTT ATCAAGTCTG GAAAACCATT CACAGCAGTC AACTCAACCA 
GAAATGCATA AATATGGTCA GTTAGTTAAA GTAGAATTAG AAGAAAATGC CGAAGATGAT 
AAAACTGAAA ACCAAATCCC TCAAAGAATG ACTAGAAACA AAGCAAATAC AATGGCAAAT 
CAAAGCAAAC AGATTCTTGC TAGCTGTACA CTATTATCAG AAAAAGACAG TGAATCCTCA 
TCTCCTAGAG GAAGAATAAG ATTAACTGAA GATGACGATC CTCAAATTCA CCATCCACGG 
AAAAGGAAAG TGTCACGTGT ACCTCAGCCT GTGCAAGTGA GTCCCTCTTT ACTACAAGCA 
AAAGAGAAAA CTCAGCAATC TCTGGCAGCC ATTGTAGATT CTCTAAAACT AGATGAGATT 
CAGCCATACA GTTCAGAGAG AGCAAATCCA TATTTTGAAT ACTTGCACAT AAGGAAAAAA 
ATAGAAGAAA AACGCAAATT ACTGTGTAGT GTGATTCCTC AAGCACCTCA GTACTATGAC 
GAATATGTAA CATTTAACGG ATCATATCTC CTGGATGGAA ACCCCTTAAG CAAGATTTGT 
ATTCCCACAA TTACACCACC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TAAGGATGAA ACTACGTTTG CAACACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAACG AACAAGAAGT TTTGCGAGTT CATTACAGAG CTGCAAGAAC ATTGGCAAAT 
CAAACACTGC CATTTAGTGC TTGTACTGTT TTGCTGGATG CCGAAGTATA CAATGTACCA 
TTGGACTCTC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAA 
TTCATGTCTT GGTTACAAGA TGTGGATGAT AAATTTGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AACATGAAGC TGCGGCTTTA AATGCTGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATTTACGA AATCCAGGAG 
TTTTATGTTC CCCTTGTTGA TGTTAACGAC GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025664.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881153.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X9, mRNA.

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CloneID	OHu50997
Clone ID Related Accession (Same CDS sequence)	XM_017025666.1 , XM_017025664.2 , XM_017025665.2 , XM_017025666.2 , XM_017025664.1 , XM_005258096.3 , XM_005258096.2 , XM_017025665.1
Accession Version	XM_017025664.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025664.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAATTC CACACCAAAT CATCCATCAC AAACAACGCC TGCCCAAAAG 
AAAACTCCCA GTTCTTCATC TCGACAGAAA GATAAAGTTA ATAAAAGAAA TGAACGTGGT 
GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA AACAAGTTAA AGAATTAATA 
AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT GGACACCACT GCATGAAGCT 
TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG CAGCTGGAGC AGATGTTAAC 
ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG CTAGTAGTGG GCACAGAGAT 
ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC AAGCTAATAA ACATGGGGAG 
CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC TACTAAAAAG AGAGGTGCCT 
TTATCTGATG ATGATGAAAG TTACACAGAT TCCGAAGAGG CTCAATCTGT AAATCCTTCT 
AGTGTTGATG AAAATATTGA CTCTGAAACA GAGAAAGACT CTCTCATCTG TGAAAGTAAA 
CAGATACTTC CCAGTAAAAC ACCTCTTCCA TCTGCCCTTG ATGAGTATGA GTTCAAAGAT 
GATGATGATG AAGAAATTAA TAAGATGATT GATGATAGGC ATATTCTTAG GAAAGAACAA 
CGAAAAGAAA ATGAACCTGA AGCAGAAAAA ACTCATTTAT TTGCAAAACA GGAGAAAGCC 
TTCTATCCTA AATCATTTAA AAGTAAAAAA CAAAAGCCAT CTAGGGTCTT ATATTCAAGT 
ACTGAAAGTT CTGATGAAGA AGCTCTTCAG AATAAAAAGA TTTCTACTTC ATGTTCCGTC 
ATCCCTGAAA CATCAAATTC TGATATGCAA ACCAAAAAGG AATATGTAGT TTCAGGTGAA 
CACAAACAGA AAGGCAAAGT TAAAAGAAAA TTGAAAAATC AGAATAAAAA TAAAGAGAAC 
CAAGAGCTAA AGCAAGAAAA GGAAGGAAAA GAAAATACAA GAATAACAAA CTTGACAGTA 
AATACTGGAC TAGATTGTTC AGAAAAGACC AGAGAGGAGG GGAACTTTAG GAAATCTTTT 
AGCCCAAAAG ATGATACTTC ATTACATTTA TTTCATATTT CCACTGGTAA ATCTCCCAAA 
CATTCTTGTG GATTAAGTGA AAAACAGTCA ACACCACTAA AACAAGAACA TACTAAAACA 
TGTTTATCAC CAGGAAGTTC TGAAATGTCA TTACAGCCTG ATCTTGTTCG GTATGATAAT 
ACAGAATCTG AATTCTTGCC AGAAAGTTCA AGTGTAAAAT CTTGTAAGCA TAAGGAAAAA 
AGCAAACATC AGAAAGATTT CCACTTAGAA TTTGGTGAAA AATCAAATGC CAAAATAAAG 
GATGAAGATC ATAGTCCAAC ATTTGAAAAT TCAGATTGCA CACTGAAAAA AATGGATAAA 
GAAGGTAAAA CATTAAAAAA ACATAAATTG AAGCATAAAG AGAGGGAAAA AGAAAAGCAT 
AAAAAAGAAA TTGAAGGTGA AAAGGAAAAA TACAAAACTA AGGATAGTGC CAAAGAACTG 
CAGAGGAGTG TGGAATTTGA TAGAGAATTT TGGAAAGAGA ATTTTTTTAA AAGTGATGAA 
ACTGAAGATC TCTTTTTAAA TATGGAACAT GAATCCTTAA CATTAGAAAA AAAATCAAAA 
TTGGAAAAAA ACATCAAAGA TGATAAATCA ACCAAGGAAA AGCATGTGTC AAAAGAGAGG 
AACTTTAAAG AGGAACGAGA CAAGATTAAA AAGGAAAGCG AGAAATCTTT TAGGGAGGAA 
AAAATAAAAG ATCTAAAAGA AGAGAGAGAA AACATACCCA CAGATAAAGA CTCAGAATTT 
ACTTCTTTGG GTATGAGTGC CATTGAGGAA TCTATAGGGC TTCATTTAGT GGAAAAGGAA 
ATAGACATTG AAAAACAAGA AAAGCATATA AAGGAAAGTA AAGAAAAACC TGAGAAGCGA 
TCTCAAATTA AAGAAAAGGA CATTGAGAAG ATGGAAAGAA AAACCTTTGA AAAAGAAAAG 
AAGATAAAAC ATGAGCATAA GTCAGAAAAA GACAAATTAG ATCTTAGTGA ATGTGTTGAT 
AAAATAAAAG AAAAGGACAA GCTATATTCG CATCACACAG AAAAATGCCA TAAAGAAGGT 
GAGAAGAGCA AAAATACTGC TGCTATTAAA AAAACTGACG ACAGAGAGAA AAGTAGAGAA 
AAGATGGATA GGAAACATGA CAAAGAAAAG CCTGAAAAAG AGAGGCATCT AGCAGAAAGC 
AAAGAAAAGC ACTTGATGGA GAAAAAAAAT AAACAATCAG ATAATAGTGA ATACAGTAAA 
TCAGAAAAAG GCAAAAATAA AGAAAAAGAC AGGGAGCTAG ATAAAAAGGA AAAATCTAGA 
GATAAAGAAA GTATAAATAT AACTAACTCC AAACACATAC AGGAAGAAAA AAAATCAAGT 
ATAGTAGACG GTAATAAAGC ACAACATGAA AAACCCTTAT CCCTTAAAGA AAAAACAAAA 
GATGAACCTT TGAAAACTCC AGATGGAAAA GAAAAAGATA AAAAAGATAA AGATATAGAT 
AGATACAAAG AACGAGACAA ACATAAAGAT AAAATTCAAA TAAATAGCTT ACTCAAACTA 
AAATCTGAAG CAGATAAGCC TAAACCTAAG TCATCACCAG CATCAAAAGA TACCCGACCT 
AAAGAAAAGA GGTTAGTGAA TGATGATTTA ATGCAGACAA GTTTTGAACG AATGCTAAGC 
CTTAAAGACC TAGAAATAGA ACAGTGGCAC AAAAAACATA AGGAAAAAAT TAAGCAAAAA 
GAAAAGGAAC GGTTGAGAAA CCGAAACTGT TTAGAACTTA AAATAAAAGA TAAAGAAAAA 
ACAAAGCATA CACCAACTGA ATCCAAAAAT AAAGAACTTA CTAGGTCAAA GAGTTCAGAA 
GTGACTGATG CATATACCAA GGAGAAACAA CCTAAAGATG CTGTAAGTAA CAGATCACAA 
TCTGTTGACA CCAAAAATGT AATGACTTTA GGGAAGTCAT CTTTTGTTTC AGATAATAGC 
TTAAACAGGT CTCCTAGATC AGAAAATGAA AAGCCGGGTC TCAGCTCCAG ATCTGTATCC 
ATGATTTCTG TTGCTAGTTC AGAAGATTCC TGCCATACTA CAGTGACAAC CCCAAGGCCT 
CCAGTTGAGT ATGACTCTGA CTTTATGTTA GAGAGTTCAG AATCCCAAAT GTCCTTTTCC 
CAGTCACCTT TTTTGTCAAT TGCCAAATCT CCTGCTCTTC ATGAAAGGGA ATTGGACAGC 
CTGGCTGACT TGCCGGAGCG GATTAAACCA CCATATGCAA ACAGACTTTC AACATCCCAT 
CTTAGGTCAT CTTCTGTAGA AGATGTTAAA CTAATTATAA GCGAGGGGAG ACCTACCATA 
GAAGTTCGAA GATGTAGCAT GCCTTCTGTC ATTTGTGAAC ATACCAAACA ATTCCAAACA 
ATATCAGAAG AGAGCAATCA AGGTAGCTTA TTAACTGTGC CAGGAGATAC TAGTCCTTCT 
CCCAAACCTG AGGTATTCTC AAATGTGCCT GAAAGAGACC TTTCAAATGT ATCTAACATA 
CATTCCAGTT TTGCAACTTC TCCAACTGGA GCTTCAAACA GCAAGTATGT TTCAGCTGAT 
AGAAATCTCA TCAAGAATAC TGCCCCAGTG AACACTGTAA TGGACAGTCC AGTGCATTTA 
GAGCCATCTA GTCAGGTTGG TGTGATCCAG AATAAATCAT GGGAGATGCC TGTTGATAGA 
CTAGAGACAT TAAGCACCAG AGACTTTATC TGCCCAAATT CTAACATACC TGATCAAGAA 
TCCTCTCTTC AGAGTTTTTG TAATTCTGAA AATAAGGTAT TGAAAGAAAA TGCTGATTTT 
TTATCCCTGC GCCAGACTGA ACTGCCAGGA AACTCTTGTG CTCAGGATCC GGCATCCTTT 
ATGCCTCCAC AGCAGCCTTG CTCTTTCCCC AGCCAATCAC TTTCAGATGC TGAATCGATT 
TCTAAACATA TGTCTTTGTC ATATGTTGCT AATCAAGAGC CAGGTATTTT ACAACAAAAA 
AATGCAGTTC AGATTATTAG TTCTGCTTTA GATACTGATA ATGAATCTAC AAAAGATACA 
GAAAATACTT TTGTCCTAGG AGATGTTCAA AAAACAGATG CCTTTGTCCC AGTGTACTCT 
GACAGCACTA TTCAAGAAGC ATCACCAAAC TTTGAGAAAG CTTATACTTT ACCTGTGTTA 
CCATCAGAAA AGGACTTTAA TGGAAGTGAT GCCTCTACCC AGCTAAATAC ACATTATGCA 
TTTAGCAAAC TAACTTACAA GTCTTCCAGT GGCCATGAAG TTGAGAATAG CACAACTGAT 
ACTCAGGTCA TTTCACATGA AAAAGAAAAC AAACTGGAGA GTTTGGTTTT AACTCATTTG 
AGTAGGTGTG ATTCTGATTT ATGTGAAATG AATGCAGGGA TGCCAAAAGG AAACCTAAAT 
GAACAAGATC CAAAACATTG TCCTGAAAGT GAAAAGTGTT TGCTTTCCAT AGAAGATGAG 
GAATCTCAAC AAAGCATTTT ATCAAGTCTG GAAAACCATT CACAGCAGTC AACTCAACCA 
GAAATGCATA AATATGGTCA GTTAGTTAAA GTAGAATTAG AAGAAAATGC CGAAGATGAT 
AAAACTGAAA ACCAAATCCC TCAAAGAATG ACTAGAAACA AAGCAAATAC AATGGCAAAT 
CAAAGCAAAC AGATTCTTGC TAGCTGTACA CTATTATCAG AAAAAGACAG TGAATCCTCA 
TCTCCTAGAG GAAGAATAAG ATTAACTGAA GATGACGATC CTCAAATTCA CCATCCACGG 
AAAAGGAAAG TGTCACGTGT ACCTCAGCCT GTGCAAGTGA GTCCCTCTTT ACTACAAGCA 
AAAGAGAAAA CTCAGCAATC TCTGGCAGCC ATTGTAGATT CTCTAAAACT AGATGAGATT 
CAGCCATACA GTTCAGAGAG AGCAAATCCA TATTTTGAAT ACTTGCACAT AAGGAAAAAA 
ATAGAAGAAA AACGCAAATT ACTGTGTAGT GTGATTCCTC AAGCACCTCA GTACTATGAC 
GAATATGTAA CATTTAACGG ATCATATCTC CTGGATGGAA ACCCCTTAAG CAAGATTTGT 
ATTCCCACAA TTACACCACC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TAAGGATGAA ACTACGTTTG CAACACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAACG AACAAGAAGT TTTGCGAGTT CATTACAGAG CTGCAAGAAC ATTGGCAAAT 
CAAACACTGC CATTTAGTGC TTGTACTGTT TTGCTGGATG CCGAAGTATA CAATGTACCA 
TTGGACTCTC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAA 
TTCATGTCTT GGTTACAAGA TGTGGATGAT AAATTTGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AACATGAAGC TGCGGCTTTA AATGCTGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATTTACGA AATCCAGGAG 
TTTTATGTTC CCCTTGTTGA TGTTAACGAC GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881153.1
CDS	350..6463
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNNSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025664.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025664.2

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAATTC CACACCAAAT CATCCATCAC AAACAACGCC TGCCCAAAAG 
AAAACTCCCA GTTCTTCATC TCGACAGAAA GATAAAGTTA ATAAAAGAAA TGAACGTGGT 
GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA AACAAGTTAA AGAATTAATA 
AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT GGACACCACT GCATGAAGCT 
TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG CAGCTGGAGC AGATGTTAAC 
ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG CTAGTAGTGG GCACAGAGAT 
ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC AAGCTAATAA ACATGGGGAG 
CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC TACTAAAAAG AGAGGTGCCT 
TTATCTGATG ATGATGAAAG TTACACAGAT TCCGAAGAGG CTCAATCTGT AAATCCTTCT 
AGTGTTGATG AAAATATTGA CTCTGAAACA GAGAAAGACT CTCTCATCTG TGAAAGTAAA 
CAGATACTTC CCAGTAAAAC ACCTCTTCCA TCTGCCCTTG ATGAGTATGA GTTCAAAGAT 
GATGATGATG AAGAAATTAA TAAGATGATT GATGATAGGC ATATTCTTAG GAAAGAACAA 
CGAAAAGAAA ATGAACCTGA AGCAGAAAAA ACTCATTTAT TTGCAAAACA GGAGAAAGCC 
TTCTATCCTA AATCATTTAA AAGTAAAAAA CAAAAGCCAT CTAGGGTCTT ATATTCAAGT 
ACTGAAAGTT CTGATGAAGA AGCTCTTCAG AATAAAAAGA TTTCTACTTC ATGTTCCGTC 
ATCCCTGAAA CATCAAATTC TGATATGCAA ACCAAAAAGG AATATGTAGT TTCAGGTGAA 
CACAAACAGA AAGGCAAAGT TAAAAGAAAA TTGAAAAATC AGAATAAAAA TAAAGAGAAC 
CAAGAGCTAA AGCAAGAAAA GGAAGGAAAA GAAAATACAA GAATAACAAA CTTGACAGTA 
AATACTGGAC TAGATTGTTC AGAAAAGACC AGAGAGGAGG GGAACTTTAG GAAATCTTTT 
AGCCCAAAAG ATGATACTTC ATTACATTTA TTTCATATTT CCACTGGTAA ATCTCCCAAA 
CATTCTTGTG GATTAAGTGA AAAACAGTCA ACACCACTAA AACAAGAACA TACTAAAACA 
TGTTTATCAC CAGGAAGTTC TGAAATGTCA TTACAGCCTG ATCTTGTTCG GTATGATAAT 
ACAGAATCTG AATTCTTGCC AGAAAGTTCA AGTGTAAAAT CTTGTAAGCA TAAGGAAAAA 
AGCAAACATC AGAAAGATTT CCACTTAGAA TTTGGTGAAA AATCAAATGC CAAAATAAAG 
GATGAAGATC ATAGTCCAAC ATTTGAAAAT TCAGATTGCA CACTGAAAAA AATGGATAAA 
GAAGGTAAAA CATTAAAAAA ACATAAATTG AAGCATAAAG AGAGGGAAAA AGAAAAGCAT 
AAAAAAGAAA TTGAAGGTGA AAAGGAAAAA TACAAAACTA AGGATAGTGC CAAAGAACTG 
CAGAGGAGTG TGGAATTTGA TAGAGAATTT TGGAAAGAGA ATTTTTTTAA AAGTGATGAA 
ACTGAAGATC TCTTTTTAAA TATGGAACAT GAATCCTTAA CATTAGAAAA AAAATCAAAA 
TTGGAAAAAA ACATCAAAGA TGATAAATCA ACCAAGGAAA AGCATGTGTC AAAAGAGAGG 
AACTTTAAAG AGGAACGAGA CAAGATTAAA AAGGAAAGCG AGAAATCTTT TAGGGAGGAA 
AAAATAAAAG ATCTAAAAGA AGAGAGAGAA AACATACCCA CAGATAAAGA CTCAGAATTT 
ACTTCTTTGG GTATGAGTGC CATTGAGGAA TCTATAGGGC TTCATTTAGT GGAAAAGGAA 
ATAGACATTG AAAAACAAGA AAAGCATATA AAGGAAAGTA AAGAAAAACC TGAGAAGCGA 
TCTCAAATTA AAGAAAAGGA CATTGAGAAG ATGGAAAGAA AAACCTTTGA AAAAGAAAAG 
AAGATAAAAC ATGAGCATAA GTCAGAAAAA GACAAATTAG ATCTTAGTGA ATGTGTTGAT 
AAAATAAAAG AAAAGGACAA GCTATATTCG CATCACACAG AAAAATGCCA TAAAGAAGGT 
GAGAAGAGCA AAAATACTGC TGCTATTAAA AAAACTGACG ACAGAGAGAA AAGTAGAGAA 
AAGATGGATA GGAAACATGA CAAAGAAAAG CCTGAAAAAG AGAGGCATCT AGCAGAAAGC 
AAAGAAAAGC ACTTGATGGA GAAAAAAAAT AAACAATCAG ATAATAGTGA ATACAGTAAA 
TCAGAAAAAG GCAAAAATAA AGAAAAAGAC AGGGAGCTAG ATAAAAAGGA AAAATCTAGA 
GATAAAGAAA GTATAAATAT AACTAACTCC AAACACATAC AGGAAGAAAA AAAATCAAGT 
ATAGTAGACG GTAATAAAGC ACAACATGAA AAACCCTTAT CCCTTAAAGA AAAAACAAAA 
GATGAACCTT TGAAAACTCC AGATGGAAAA GAAAAAGATA AAAAAGATAA AGATATAGAT 
AGATACAAAG AACGAGACAA ACATAAAGAT AAAATTCAAA TAAATAGCTT ACTCAAACTA 
AAATCTGAAG CAGATAAGCC TAAACCTAAG TCATCACCAG CATCAAAAGA TACCCGACCT 
AAAGAAAAGA GGTTAGTGAA TGATGATTTA ATGCAGACAA GTTTTGAACG AATGCTAAGC 
CTTAAAGACC TAGAAATAGA ACAGTGGCAC AAAAAACATA AGGAAAAAAT TAAGCAAAAA 
GAAAAGGAAC GGTTGAGAAA CCGAAACTGT TTAGAACTTA AAATAAAAGA TAAAGAAAAA 
ACAAAGCATA CACCAACTGA ATCCAAAAAT AAAGAACTTA CTAGGTCAAA GAGTTCAGAA 
GTGACTGATG CATATACCAA GGAGAAACAA CCTAAAGATG CTGTAAGTAA CAGATCACAA 
TCTGTTGACA CCAAAAATGT AATGACTTTA GGGAAGTCAT CTTTTGTTTC AGATAATAGC 
TTAAACAGGT CTCCTAGATC AGAAAATGAA AAGCCGGGTC TCAGCTCCAG ATCTGTATCC 
ATGATTTCTG TTGCTAGTTC AGAAGATTCC TGCCATACTA CAGTGACAAC CCCAAGGCCT 
CCAGTTGAGT ATGACTCTGA CTTTATGTTA GAGAGTTCAG AATCCCAAAT GTCCTTTTCC 
CAGTCACCTT TTTTGTCAAT TGCCAAATCT CCTGCTCTTC ATGAAAGGGA ATTGGACAGC 
CTGGCTGACT TGCCGGAGCG GATTAAACCA CCATATGCAA ACAGACTTTC AACATCCCAT 
CTTAGGTCAT CTTCTGTAGA AGATGTTAAA CTAATTATAA GCGAGGGGAG ACCTACCATA 
GAAGTTCGAA GATGTAGCAT GCCTTCTGTC ATTTGTGAAC ATACCAAACA ATTCCAAACA 
ATATCAGAAG AGAGCAATCA AGGTAGCTTA TTAACTGTGC CAGGAGATAC TAGTCCTTCT 
CCCAAACCTG AGGTATTCTC AAATGTGCCT GAAAGAGACC TTTCAAATGT ATCTAACATA 
CATTCCAGTT TTGCAACTTC TCCAACTGGA GCTTCAAACA GCAAGTATGT TTCAGCTGAT 
AGAAATCTCA TCAAGAATAC TGCCCCAGTG AACACTGTAA TGGACAGTCC AGTGCATTTA 
GAGCCATCTA GTCAGGTTGG TGTGATCCAG AATAAATCAT GGGAGATGCC TGTTGATAGA 
CTAGAGACAT TAAGCACCAG AGACTTTATC TGCCCAAATT CTAACATACC TGATCAAGAA 
TCCTCTCTTC AGAGTTTTTG TAATTCTGAA AATAAGGTAT TGAAAGAAAA TGCTGATTTT 
TTATCCCTGC GCCAGACTGA ACTGCCAGGA AACTCTTGTG CTCAGGATCC GGCATCCTTT 
ATGCCTCCAC AGCAGCCTTG CTCTTTCCCC AGCCAATCAC TTTCAGATGC TGAATCGATT 
TCTAAACATA TGTCTTTGTC ATATGTTGCT AATCAAGAGC CAGGTATTTT ACAACAAAAA 
AATGCAGTTC AGATTATTAG TTCTGCTTTA GATACTGATA ATGAATCTAC AAAAGATACA 
GAAAATACTT TTGTCCTAGG AGATGTTCAA AAAACAGATG CCTTTGTCCC AGTGTACTCT 
GACAGCACTA TTCAAGAAGC ATCACCAAAC TTTGAGAAAG CTTATACTTT ACCTGTGTTA 
CCATCAGAAA AGGACTTTAA TGGAAGTGAT GCCTCTACCC AGCTAAATAC ACATTATGCA 
TTTAGCAAAC TAACTTACAA GTCTTCCAGT GGCCATGAAG TTGAGAATAG CACAACTGAT 
ACTCAGGTCA TTTCACATGA AAAAGAAAAC AAACTGGAGA GTTTGGTTTT AACTCATTTG 
AGTAGGTGTG ATTCTGATTT ATGTGAAATG AATGCAGGGA TGCCAAAAGG AAACCTAAAT 
GAACAAGATC CAAAACATTG TCCTGAAAGT GAAAAGTGTT TGCTTTCCAT AGAAGATGAG 
GAATCTCAAC AAAGCATTTT ATCAAGTCTG GAAAACCATT CACAGCAGTC AACTCAACCA 
GAAATGCATA AATATGGTCA GTTAGTTAAA GTAGAATTAG AAGAAAATGC CGAAGATGAT 
AAAACTGAAA ACCAAATCCC TCAAAGAATG ACTAGAAACA AAGCAAATAC AATGGCAAAT 
CAAAGCAAAC AGATTCTTGC TAGCTGTACA CTATTATCAG AAAAAGACAG TGAATCCTCA 
TCTCCTAGAG GAAGAATAAG ATTAACTGAA GATGACGATC CTCAAATTCA CCATCCACGG 
AAAAGGAAAG TGTCACGTGT ACCTCAGCCT GTGCAAGTGA GTCCCTCTTT ACTACAAGCA 
AAAGAGAAAA CTCAGCAATC TCTGGCAGCC ATTGTAGATT CTCTAAAACT AGATGAGATT 
CAGCCATACA GTTCAGAGAG AGCAAATCCA TATTTTGAAT ACTTGCACAT AAGGAAAAAA 
ATAGAAGAAA AACGCAAATT ACTGTGTAGT GTGATTCCTC AAGCACCTCA GTACTATGAC 
GAATATGTAA CATTTAACGG ATCATATCTC CTGGATGGAA ACCCCTTAAG CAAGATTTGT 
ATTCCCACAA TTACACCACC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TAAGGATGAA ACTACGTTTG CAACACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAACG AACAAGAAGT TTTGCGAGTT CATTACAGAG CTGCAAGAAC ATTGGCAAAT 
CAAACACTGC CATTTAGTGC TTGTACTGTT TTGCTGGATG CCGAAGTATA CAATGTACCA 
TTGGACTCTC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAA 
TTCATGTCTT GGTTACAAGA TGTGGATGAT AAATTTGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AACATGAAGC TGCGGCTTTA AATGCTGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATTTACGA AATCCAGGAG 
TTTTATGTTC CCCTTGTTGA TGTTAACGAC GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025665.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881154.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X10, mRNA.

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CloneID	OHu50997
Clone ID Related Accession (Same CDS sequence)	XM_017025666.1 , XM_017025664.2 , XM_017025665.2 , XM_017025666.2 , XM_017025664.1 , XM_005258096.3 , XM_005258096.2 , XM_017025665.1
Accession Version	XM_017025665.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025665.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAATTC CACACCAAAT CATCCATCAC AAACAACGCC TGCCCAAAAG 
AAAACTCCCA GTTCTTCATC TCGACAGAAA GATAAAGTTA ATAAAAGAAA TGAACGTGGT 
GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA AACAAGTTAA AGAATTAATA 
AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT GGACACCACT GCATGAAGCT 
TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG CAGCTGGAGC AGATGTTAAC 
ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG CTAGTAGTGG GCACAGAGAT 
ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC AAGCTAATAA ACATGGGGAG 
CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC TACTAAAAAG AGAGGTGCCT 
TTATCTGATG ATGATGAAAG TTACACAGAT TCCGAAGAGG CTCAATCTGT AAATCCTTCT 
AGTGTTGATG AAAATATTGA CTCTGAAACA GAGAAAGACT CTCTCATCTG TGAAAGTAAA 
CAGATACTTC CCAGTAAAAC ACCTCTTCCA TCTGCCCTTG ATGAGTATGA GTTCAAAGAT 
GATGATGATG AAGAAATTAA TAAGATGATT GATGATAGGC ATATTCTTAG GAAAGAACAA 
CGAAAAGAAA ATGAACCTGA AGCAGAAAAA ACTCATTTAT TTGCAAAACA GGAGAAAGCC 
TTCTATCCTA AATCATTTAA AAGTAAAAAA CAAAAGCCAT CTAGGGTCTT ATATTCAAGT 
ACTGAAAGTT CTGATGAAGA AGCTCTTCAG AATAAAAAGA TTTCTACTTC ATGTTCCGTC 
ATCCCTGAAA CATCAAATTC TGATATGCAA ACCAAAAAGG AATATGTAGT TTCAGGTGAA 
CACAAACAGA AAGGCAAAGT TAAAAGAAAA TTGAAAAATC AGAATAAAAA TAAAGAGAAC 
CAAGAGCTAA AGCAAGAAAA GGAAGGAAAA GAAAATACAA GAATAACAAA CTTGACAGTA 
AATACTGGAC TAGATTGTTC AGAAAAGACC AGAGAGGAGG GGAACTTTAG GAAATCTTTT 
AGCCCAAAAG ATGATACTTC ATTACATTTA TTTCATATTT CCACTGGTAA ATCTCCCAAA 
CATTCTTGTG GATTAAGTGA AAAACAGTCA ACACCACTAA AACAAGAACA TACTAAAACA 
TGTTTATCAC CAGGAAGTTC TGAAATGTCA TTACAGCCTG ATCTTGTTCG GTATGATAAT 
ACAGAATCTG AATTCTTGCC AGAAAGTTCA AGTGTAAAAT CTTGTAAGCA TAAGGAAAAA 
AGCAAACATC AGAAAGATTT CCACTTAGAA TTTGGTGAAA AATCAAATGC CAAAATAAAG 
GATGAAGATC ATAGTCCAAC ATTTGAAAAT TCAGATTGCA CACTGAAAAA AATGGATAAA 
GAAGGTAAAA CATTAAAAAA ACATAAATTG AAGCATAAAG AGAGGGAAAA AGAAAAGCAT 
AAAAAAGAAA TTGAAGGTGA AAAGGAAAAA TACAAAACTA AGGATAGTGC CAAAGAACTG 
CAGAGGAGTG TGGAATTTGA TAGAGAATTT TGGAAAGAGA ATTTTTTTAA AAGTGATGAA 
ACTGAAGATC TCTTTTTAAA TATGGAACAT GAATCCTTAA CATTAGAAAA AAAATCAAAA 
TTGGAAAAAA ACATCAAAGA TGATAAATCA ACCAAGGAAA AGCATGTGTC AAAAGAGAGG 
AACTTTAAAG AGGAACGAGA CAAGATTAAA AAGGAAAGCG AGAAATCTTT TAGGGAGGAA 
AAAATAAAAG ATCTAAAAGA AGAGAGAGAA AACATACCCA CAGATAAAGA CTCAGAATTT 
ACTTCTTTGG GTATGAGTGC CATTGAGGAA TCTATAGGGC TTCATTTAGT GGAAAAGGAA 
ATAGACATTG AAAAACAAGA AAAGCATATA AAGGAAAGTA AAGAAAAACC TGAGAAGCGA 
TCTCAAATTA AAGAAAAGGA CATTGAGAAG ATGGAAAGAA AAACCTTTGA AAAAGAAAAG 
AAGATAAAAC ATGAGCATAA GTCAGAAAAA GACAAATTAG ATCTTAGTGA ATGTGTTGAT 
AAAATAAAAG AAAAGGACAA GCTATATTCG CATCACACAG AAAAATGCCA TAAAGAAGGT 
GAGAAGAGCA AAAATACTGC TGCTATTAAA AAAACTGACG ACAGAGAGAA AAGTAGAGAA 
AAGATGGATA GGAAACATGA CAAAGAAAAG CCTGAAAAAG AGAGGCATCT AGCAGAAAGC 
AAAGAAAAGC ACTTGATGGA GAAAAAAAAT AAACAATCAG ATAATAGTGA ATACAGTAAA 
TCAGAAAAAG GCAAAAATAA AGAAAAAGAC AGGGAGCTAG ATAAAAAGGA AAAATCTAGA 
GATAAAGAAA GTATAAATAT AACTAACTCC AAACACATAC AGGAAGAAAA AAAATCAAGT 
ATAGTAGACG GTAATAAAGC ACAACATGAA AAACCCTTAT CCCTTAAAGA AAAAACAAAA 
GATGAACCTT TGAAAACTCC AGATGGAAAA GAAAAAGATA AAAAAGATAA AGATATAGAT 
AGATACAAAG AACGAGACAA ACATAAAGAT AAAATTCAAA TAAATAGCTT ACTCAAACTA 
AAATCTGAAG CAGATAAGCC TAAACCTAAG TCATCACCAG CATCAAAAGA TACCCGACCT 
AAAGAAAAGA GGTTAGTGAA TGATGATTTA ATGCAGACAA GTTTTGAACG AATGCTAAGC 
CTTAAAGACC TAGAAATAGA ACAGTGGCAC AAAAAACATA AGGAAAAAAT TAAGCAAAAA 
GAAAAGGAAC GGTTGAGAAA CCGAAACTGT TTAGAACTTA AAATAAAAGA TAAAGAAAAA 
ACAAAGCATA CACCAACTGA ATCCAAAAAT AAAGAACTTA CTAGGTCAAA GAGTTCAGAA 
GTGACTGATG CATATACCAA GGAGAAACAA CCTAAAGATG CTGTAAGTAA CAGATCACAA 
TCTGTTGACA CCAAAAATGT AATGACTTTA GGGAAGTCAT CTTTTGTTTC AGATAATAGC 
TTAAACAGGT CTCCTAGATC AGAAAATGAA AAGCCGGGTC TCAGCTCCAG ATCTGTATCC 
ATGATTTCTG TTGCTAGTTC AGAAGATTCC TGCCATACTA CAGTGACAAC CCCAAGGCCT 
CCAGTTGAGT ATGACTCTGA CTTTATGTTA GAGAGTTCAG AATCCCAAAT GTCCTTTTCC 
CAGTCACCTT TTTTGTCAAT TGCCAAATCT CCTGCTCTTC ATGAAAGGGA ATTGGACAGC 
CTGGCTGACT TGCCGGAGCG GATTAAACCA CCATATGCAA ACAGACTTTC AACATCCCAT 
CTTAGGTCAT CTTCTGTAGA AGATGTTAAA CTAATTATAA GCGAGGGGAG ACCTACCATA 
GAAGTTCGAA GATGTAGCAT GCCTTCTGTC ATTTGTGAAC ATACCAAACA ATTCCAAACA 
ATATCAGAAG AGAGCAATCA AGGTAGCTTA TTAACTGTGC CAGGAGATAC TAGTCCTTCT 
CCCAAACCTG AGGTATTCTC AAATGTGCCT GAAAGAGACC TTTCAAATGT ATCTAACATA 
CATTCCAGTT TTGCAACTTC TCCAACTGGA GCTTCAAACA GCAAGTATGT TTCAGCTGAT 
AGAAATCTCA TCAAGAATAC TGCCCCAGTG AACACTGTAA TGGACAGTCC AGTGCATTTA 
GAGCCATCTA GTCAGGTTGG TGTGATCCAG AATAAATCAT GGGAGATGCC TGTTGATAGA 
CTAGAGACAT TAAGCACCAG AGACTTTATC TGCCCAAATT CTAACATACC TGATCAAGAA 
TCCTCTCTTC AGAGTTTTTG TAATTCTGAA AATAAGGTAT TGAAAGAAAA TGCTGATTTT 
TTATCCCTGC GCCAGACTGA ACTGCCAGGA AACTCTTGTG CTCAGGATCC GGCATCCTTT 
ATGCCTCCAC AGCAGCCTTG CTCTTTCCCC AGCCAATCAC TTTCAGATGC TGAATCGATT 
TCTAAACATA TGTCTTTGTC ATATGTTGCT AATCAAGAGC CAGGTATTTT ACAACAAAAA 
AATGCAGTTC AGATTATTAG TTCTGCTTTA GATACTGATA ATGAATCTAC AAAAGATACA 
GAAAATACTT TTGTCCTAGG AGATGTTCAA AAAACAGATG CCTTTGTCCC AGTGTACTCT 
GACAGCACTA TTCAAGAAGC ATCACCAAAC TTTGAGAAAG CTTATACTTT ACCTGTGTTA 
CCATCAGAAA AGGACTTTAA TGGAAGTGAT GCCTCTACCC AGCTAAATAC ACATTATGCA 
TTTAGCAAAC TAACTTACAA GTCTTCCAGT GGCCATGAAG TTGAGAATAG CACAACTGAT 
ACTCAGGTCA TTTCACATGA AAAAGAAAAC AAACTGGAGA GTTTGGTTTT AACTCATTTG 
AGTAGGTGTG ATTCTGATTT ATGTGAAATG AATGCAGGGA TGCCAAAAGG AAACCTAAAT 
GAACAAGATC CAAAACATTG TCCTGAAAGT GAAAAGTGTT TGCTTTCCAT AGAAGATGAG 
GAATCTCAAC AAAGCATTTT ATCAAGTCTG GAAAACCATT CACAGCAGTC AACTCAACCA 
GAAATGCATA AATATGGTCA GTTAGTTAAA GTAGAATTAG AAGAAAATGC CGAAGATGAT 
AAAACTGAAA ACCAAATCCC TCAAAGAATG ACTAGAAACA AAGCAAATAC AATGGCAAAT 
CAAAGCAAAC AGATTCTTGC TAGCTGTACA CTATTATCAG AAAAAGACAG TGAATCCTCA 
TCTCCTAGAG GAAGAATAAG ATTAACTGAA GATGACGATC CTCAAATTCA CCATCCACGG 
AAAAGGAAAG TGTCACGTGT ACCTCAGCCT GTGCAAGTGA GTCCCTCTTT ACTACAAGCA 
AAAGAGAAAA CTCAGCAATC TCTGGCAGCC ATTGTAGATT CTCTAAAACT AGATGAGATT 
CAGCCATACA GTTCAGAGAG AGCAAATCCA TATTTTGAAT ACTTGCACAT AAGGAAAAAA 
ATAGAAGAAA AACGCAAATT ACTGTGTAGT GTGATTCCTC AAGCACCTCA GTACTATGAC 
GAATATGTAA CATTTAACGG ATCATATCTC CTGGATGGAA ACCCCTTAAG CAAGATTTGT 
ATTCCCACAA TTACACCACC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TAAGGATGAA ACTACGTTTG CAACACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAACG AACAAGAAGT TTTGCGAGTT CATTACAGAG CTGCAAGAAC ATTGGCAAAT 
CAAACACTGC CATTTAGTGC TTGTACTGTT TTGCTGGATG CCGAAGTATA CAATGTACCA 
TTGGACTCTC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAA 
TTCATGTCTT GGTTACAAGA TGTGGATGAT AAATTTGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AACATGAAGC TGCGGCTTTA AATGCTGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATTTACGA AATCCAGGAG 
TTTTATGTTC CCCTTGTTGA TGTTAACGAC GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881154.1
CDS	384..6497
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNNSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025665.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025665.2

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAATTC CACACCAAAT CATCCATCAC AAACAACGCC TGCCCAAAAG 
AAAACTCCCA GTTCTTCATC TCGACAGAAA GATAAAGTTA ATAAAAGAAA TGAACGTGGT 
GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA AACAAGTTAA AGAATTAATA 
AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT GGACACCACT GCATGAAGCT 
TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG CAGCTGGAGC AGATGTTAAC 
ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG CTAGTAGTGG GCACAGAGAT 
ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC AAGCTAATAA ACATGGGGAG 
CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC TACTAAAAAG AGAGGTGCCT 
TTATCTGATG ATGATGAAAG TTACACAGAT TCCGAAGAGG CTCAATCTGT AAATCCTTCT 
AGTGTTGATG AAAATATTGA CTCTGAAACA GAGAAAGACT CTCTCATCTG TGAAAGTAAA 
CAGATACTTC CCAGTAAAAC ACCTCTTCCA TCTGCCCTTG ATGAGTATGA GTTCAAAGAT 
GATGATGATG AAGAAATTAA TAAGATGATT GATGATAGGC ATATTCTTAG GAAAGAACAA 
CGAAAAGAAA ATGAACCTGA AGCAGAAAAA ACTCATTTAT TTGCAAAACA GGAGAAAGCC 
TTCTATCCTA AATCATTTAA AAGTAAAAAA CAAAAGCCAT CTAGGGTCTT ATATTCAAGT 
ACTGAAAGTT CTGATGAAGA AGCTCTTCAG AATAAAAAGA TTTCTACTTC ATGTTCCGTC 
ATCCCTGAAA CATCAAATTC TGATATGCAA ACCAAAAAGG AATATGTAGT TTCAGGTGAA 
CACAAACAGA AAGGCAAAGT TAAAAGAAAA TTGAAAAATC AGAATAAAAA TAAAGAGAAC 
CAAGAGCTAA AGCAAGAAAA GGAAGGAAAA GAAAATACAA GAATAACAAA CTTGACAGTA 
AATACTGGAC TAGATTGTTC AGAAAAGACC AGAGAGGAGG GGAACTTTAG GAAATCTTTT 
AGCCCAAAAG ATGATACTTC ATTACATTTA TTTCATATTT CCACTGGTAA ATCTCCCAAA 
CATTCTTGTG GATTAAGTGA AAAACAGTCA ACACCACTAA AACAAGAACA TACTAAAACA 
TGTTTATCAC CAGGAAGTTC TGAAATGTCA TTACAGCCTG ATCTTGTTCG GTATGATAAT 
ACAGAATCTG AATTCTTGCC AGAAAGTTCA AGTGTAAAAT CTTGTAAGCA TAAGGAAAAA 
AGCAAACATC AGAAAGATTT CCACTTAGAA TTTGGTGAAA AATCAAATGC CAAAATAAAG 
GATGAAGATC ATAGTCCAAC ATTTGAAAAT TCAGATTGCA CACTGAAAAA AATGGATAAA 
GAAGGTAAAA CATTAAAAAA ACATAAATTG AAGCATAAAG AGAGGGAAAA AGAAAAGCAT 
AAAAAAGAAA TTGAAGGTGA AAAGGAAAAA TACAAAACTA AGGATAGTGC CAAAGAACTG 
CAGAGGAGTG TGGAATTTGA TAGAGAATTT TGGAAAGAGA ATTTTTTTAA AAGTGATGAA 
ACTGAAGATC TCTTTTTAAA TATGGAACAT GAATCCTTAA CATTAGAAAA AAAATCAAAA 
TTGGAAAAAA ACATCAAAGA TGATAAATCA ACCAAGGAAA AGCATGTGTC AAAAGAGAGG 
AACTTTAAAG AGGAACGAGA CAAGATTAAA AAGGAAAGCG AGAAATCTTT TAGGGAGGAA 
AAAATAAAAG ATCTAAAAGA AGAGAGAGAA AACATACCCA CAGATAAAGA CTCAGAATTT 
ACTTCTTTGG GTATGAGTGC CATTGAGGAA TCTATAGGGC TTCATTTAGT GGAAAAGGAA 
ATAGACATTG AAAAACAAGA AAAGCATATA AAGGAAAGTA AAGAAAAACC TGAGAAGCGA 
TCTCAAATTA AAGAAAAGGA CATTGAGAAG ATGGAAAGAA AAACCTTTGA AAAAGAAAAG 
AAGATAAAAC ATGAGCATAA GTCAGAAAAA GACAAATTAG ATCTTAGTGA ATGTGTTGAT 
AAAATAAAAG AAAAGGACAA GCTATATTCG CATCACACAG AAAAATGCCA TAAAGAAGGT 
GAGAAGAGCA AAAATACTGC TGCTATTAAA AAAACTGACG ACAGAGAGAA AAGTAGAGAA 
AAGATGGATA GGAAACATGA CAAAGAAAAG CCTGAAAAAG AGAGGCATCT AGCAGAAAGC 
AAAGAAAAGC ACTTGATGGA GAAAAAAAAT AAACAATCAG ATAATAGTGA ATACAGTAAA 
TCAGAAAAAG GCAAAAATAA AGAAAAAGAC AGGGAGCTAG ATAAAAAGGA AAAATCTAGA 
GATAAAGAAA GTATAAATAT AACTAACTCC AAACACATAC AGGAAGAAAA AAAATCAAGT 
ATAGTAGACG GTAATAAAGC ACAACATGAA AAACCCTTAT CCCTTAAAGA AAAAACAAAA 
GATGAACCTT TGAAAACTCC AGATGGAAAA GAAAAAGATA AAAAAGATAA AGATATAGAT 
AGATACAAAG AACGAGACAA ACATAAAGAT AAAATTCAAA TAAATAGCTT ACTCAAACTA 
AAATCTGAAG CAGATAAGCC TAAACCTAAG TCATCACCAG CATCAAAAGA TACCCGACCT 
AAAGAAAAGA GGTTAGTGAA TGATGATTTA ATGCAGACAA GTTTTGAACG AATGCTAAGC 
CTTAAAGACC TAGAAATAGA ACAGTGGCAC AAAAAACATA AGGAAAAAAT TAAGCAAAAA 
GAAAAGGAAC GGTTGAGAAA CCGAAACTGT TTAGAACTTA AAATAAAAGA TAAAGAAAAA 
ACAAAGCATA CACCAACTGA ATCCAAAAAT AAAGAACTTA CTAGGTCAAA GAGTTCAGAA 
GTGACTGATG CATATACCAA GGAGAAACAA CCTAAAGATG CTGTAAGTAA CAGATCACAA 
TCTGTTGACA CCAAAAATGT AATGACTTTA GGGAAGTCAT CTTTTGTTTC AGATAATAGC 
TTAAACAGGT CTCCTAGATC AGAAAATGAA AAGCCGGGTC TCAGCTCCAG ATCTGTATCC 
ATGATTTCTG TTGCTAGTTC AGAAGATTCC TGCCATACTA CAGTGACAAC CCCAAGGCCT 
CCAGTTGAGT ATGACTCTGA CTTTATGTTA GAGAGTTCAG AATCCCAAAT GTCCTTTTCC 
CAGTCACCTT TTTTGTCAAT TGCCAAATCT CCTGCTCTTC ATGAAAGGGA ATTGGACAGC 
CTGGCTGACT TGCCGGAGCG GATTAAACCA CCATATGCAA ACAGACTTTC AACATCCCAT 
CTTAGGTCAT CTTCTGTAGA AGATGTTAAA CTAATTATAA GCGAGGGGAG ACCTACCATA 
GAAGTTCGAA GATGTAGCAT GCCTTCTGTC ATTTGTGAAC ATACCAAACA ATTCCAAACA 
ATATCAGAAG AGAGCAATCA AGGTAGCTTA TTAACTGTGC CAGGAGATAC TAGTCCTTCT 
CCCAAACCTG AGGTATTCTC AAATGTGCCT GAAAGAGACC TTTCAAATGT ATCTAACATA 
CATTCCAGTT TTGCAACTTC TCCAACTGGA GCTTCAAACA GCAAGTATGT TTCAGCTGAT 
AGAAATCTCA TCAAGAATAC TGCCCCAGTG AACACTGTAA TGGACAGTCC AGTGCATTTA 
GAGCCATCTA GTCAGGTTGG TGTGATCCAG AATAAATCAT GGGAGATGCC TGTTGATAGA 
CTAGAGACAT TAAGCACCAG AGACTTTATC TGCCCAAATT CTAACATACC TGATCAAGAA 
TCCTCTCTTC AGAGTTTTTG TAATTCTGAA AATAAGGTAT TGAAAGAAAA TGCTGATTTT 
TTATCCCTGC GCCAGACTGA ACTGCCAGGA AACTCTTGTG CTCAGGATCC GGCATCCTTT 
ATGCCTCCAC AGCAGCCTTG CTCTTTCCCC AGCCAATCAC TTTCAGATGC TGAATCGATT 
TCTAAACATA TGTCTTTGTC ATATGTTGCT AATCAAGAGC CAGGTATTTT ACAACAAAAA 
AATGCAGTTC AGATTATTAG TTCTGCTTTA GATACTGATA ATGAATCTAC AAAAGATACA 
GAAAATACTT TTGTCCTAGG AGATGTTCAA AAAACAGATG CCTTTGTCCC AGTGTACTCT 
GACAGCACTA TTCAAGAAGC ATCACCAAAC TTTGAGAAAG CTTATACTTT ACCTGTGTTA 
CCATCAGAAA AGGACTTTAA TGGAAGTGAT GCCTCTACCC AGCTAAATAC ACATTATGCA 
TTTAGCAAAC TAACTTACAA GTCTTCCAGT GGCCATGAAG TTGAGAATAG CACAACTGAT 
ACTCAGGTCA TTTCACATGA AAAAGAAAAC AAACTGGAGA GTTTGGTTTT AACTCATTTG 
AGTAGGTGTG ATTCTGATTT ATGTGAAATG AATGCAGGGA TGCCAAAAGG AAACCTAAAT 
GAACAAGATC CAAAACATTG TCCTGAAAGT GAAAAGTGTT TGCTTTCCAT AGAAGATGAG 
GAATCTCAAC AAAGCATTTT ATCAAGTCTG GAAAACCATT CACAGCAGTC AACTCAACCA 
GAAATGCATA AATATGGTCA GTTAGTTAAA GTAGAATTAG AAGAAAATGC CGAAGATGAT 
AAAACTGAAA ACCAAATCCC TCAAAGAATG ACTAGAAACA AAGCAAATAC AATGGCAAAT 
CAAAGCAAAC AGATTCTTGC TAGCTGTACA CTATTATCAG AAAAAGACAG TGAATCCTCA 
TCTCCTAGAG GAAGAATAAG ATTAACTGAA GATGACGATC CTCAAATTCA CCATCCACGG 
AAAAGGAAAG TGTCACGTGT ACCTCAGCCT GTGCAAGTGA GTCCCTCTTT ACTACAAGCA 
AAAGAGAAAA CTCAGCAATC TCTGGCAGCC ATTGTAGATT CTCTAAAACT AGATGAGATT 
CAGCCATACA GTTCAGAGAG AGCAAATCCA TATTTTGAAT ACTTGCACAT AAGGAAAAAA 
ATAGAAGAAA AACGCAAATT ACTGTGTAGT GTGATTCCTC AAGCACCTCA GTACTATGAC 
GAATATGTAA CATTTAACGG ATCATATCTC CTGGATGGAA ACCCCTTAAG CAAGATTTGT 
ATTCCCACAA TTACACCACC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TAAGGATGAA ACTACGTTTG CAACACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAACG AACAAGAAGT TTTGCGAGTT CATTACAGAG CTGCAAGAAC ATTGGCAAAT 
CAAACACTGC CATTTAGTGC TTGTACTGTT TTGCTGGATG CCGAAGTATA CAATGTACCA 
TTGGACTCTC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAA 
TTCATGTCTT GGTTACAAGA TGTGGATGAT AAATTTGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AACATGAAGC TGCGGCTTTA AATGCTGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATTTACGA AATCCAGGAG 
TTTTATGTTC CCCTTGTTGA TGTTAACGAC GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025666.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881155.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X11, mRNA.

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CloneID	OHu50997
Clone ID Related Accession (Same CDS sequence)	XM_017025666.1 , XM_017025664.2 , XM_017025665.2 , XM_017025666.2 , XM_017025664.1 , XM_005258096.3 , XM_005258096.2 , XM_017025665.1
Accession Version	XM_017025666.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025666.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAATTC CACACCAAAT CATCCATCAC AAACAACGCC TGCCCAAAAG 
AAAACTCCCA GTTCTTCATC TCGACAGAAA GATAAAGTTA ATAAAAGAAA TGAACGTGGT 
GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA AACAAGTTAA AGAATTAATA 
AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT GGACACCACT GCATGAAGCT 
TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG CAGCTGGAGC AGATGTTAAC 
ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG CTAGTAGTGG GCACAGAGAT 
ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC AAGCTAATAA ACATGGGGAG 
CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC TACTAAAAAG AGAGGTGCCT 
TTATCTGATG ATGATGAAAG TTACACAGAT TCCGAAGAGG CTCAATCTGT AAATCCTTCT 
AGTGTTGATG AAAATATTGA CTCTGAAACA GAGAAAGACT CTCTCATCTG TGAAAGTAAA 
CAGATACTTC CCAGTAAAAC ACCTCTTCCA TCTGCCCTTG ATGAGTATGA GTTCAAAGAT 
GATGATGATG AAGAAATTAA TAAGATGATT GATGATAGGC ATATTCTTAG GAAAGAACAA 
CGAAAAGAAA ATGAACCTGA AGCAGAAAAA ACTCATTTAT TTGCAAAACA GGAGAAAGCC 
TTCTATCCTA AATCATTTAA AAGTAAAAAA CAAAAGCCAT CTAGGGTCTT ATATTCAAGT 
ACTGAAAGTT CTGATGAAGA AGCTCTTCAG AATAAAAAGA TTTCTACTTC ATGTTCCGTC 
ATCCCTGAAA CATCAAATTC TGATATGCAA ACCAAAAAGG AATATGTAGT TTCAGGTGAA 
CACAAACAGA AAGGCAAAGT TAAAAGAAAA TTGAAAAATC AGAATAAAAA TAAAGAGAAC 
CAAGAGCTAA AGCAAGAAAA GGAAGGAAAA GAAAATACAA GAATAACAAA CTTGACAGTA 
AATACTGGAC TAGATTGTTC AGAAAAGACC AGAGAGGAGG GGAACTTTAG GAAATCTTTT 
AGCCCAAAAG ATGATACTTC ATTACATTTA TTTCATATTT CCACTGGTAA ATCTCCCAAA 
CATTCTTGTG GATTAAGTGA AAAACAGTCA ACACCACTAA AACAAGAACA TACTAAAACA 
TGTTTATCAC CAGGAAGTTC TGAAATGTCA TTACAGCCTG ATCTTGTTCG GTATGATAAT 
ACAGAATCTG AATTCTTGCC AGAAAGTTCA AGTGTAAAAT CTTGTAAGCA TAAGGAAAAA 
AGCAAACATC AGAAAGATTT CCACTTAGAA TTTGGTGAAA AATCAAATGC CAAAATAAAG 
GATGAAGATC ATAGTCCAAC ATTTGAAAAT TCAGATTGCA CACTGAAAAA AATGGATAAA 
GAAGGTAAAA CATTAAAAAA ACATAAATTG AAGCATAAAG AGAGGGAAAA AGAAAAGCAT 
AAAAAAGAAA TTGAAGGTGA AAAGGAAAAA TACAAAACTA AGGATAGTGC CAAAGAACTG 
CAGAGGAGTG TGGAATTTGA TAGAGAATTT TGGAAAGAGA ATTTTTTTAA AAGTGATGAA 
ACTGAAGATC TCTTTTTAAA TATGGAACAT GAATCCTTAA CATTAGAAAA AAAATCAAAA 
TTGGAAAAAA ACATCAAAGA TGATAAATCA ACCAAGGAAA AGCATGTGTC AAAAGAGAGG 
AACTTTAAAG AGGAACGAGA CAAGATTAAA AAGGAAAGCG AGAAATCTTT TAGGGAGGAA 
AAAATAAAAG ATCTAAAAGA AGAGAGAGAA AACATACCCA CAGATAAAGA CTCAGAATTT 
ACTTCTTTGG GTATGAGTGC CATTGAGGAA TCTATAGGGC TTCATTTAGT GGAAAAGGAA 
ATAGACATTG AAAAACAAGA AAAGCATATA AAGGAAAGTA AAGAAAAACC TGAGAAGCGA 
TCTCAAATTA AAGAAAAGGA CATTGAGAAG ATGGAAAGAA AAACCTTTGA AAAAGAAAAG 
AAGATAAAAC ATGAGCATAA GTCAGAAAAA GACAAATTAG ATCTTAGTGA ATGTGTTGAT 
AAAATAAAAG AAAAGGACAA GCTATATTCG CATCACACAG AAAAATGCCA TAAAGAAGGT 
GAGAAGAGCA AAAATACTGC TGCTATTAAA AAAACTGACG ACAGAGAGAA AAGTAGAGAA 
AAGATGGATA GGAAACATGA CAAAGAAAAG CCTGAAAAAG AGAGGCATCT AGCAGAAAGC 
AAAGAAAAGC ACTTGATGGA GAAAAAAAAT AAACAATCAG ATAATAGTGA ATACAGTAAA 
TCAGAAAAAG GCAAAAATAA AGAAAAAGAC AGGGAGCTAG ATAAAAAGGA AAAATCTAGA 
GATAAAGAAA GTATAAATAT AACTAACTCC AAACACATAC AGGAAGAAAA AAAATCAAGT 
ATAGTAGACG GTAATAAAGC ACAACATGAA AAACCCTTAT CCCTTAAAGA AAAAACAAAA 
GATGAACCTT TGAAAACTCC AGATGGAAAA GAAAAAGATA AAAAAGATAA AGATATAGAT 
AGATACAAAG AACGAGACAA ACATAAAGAT AAAATTCAAA TAAATAGCTT ACTCAAACTA 
AAATCTGAAG CAGATAAGCC TAAACCTAAG TCATCACCAG CATCAAAAGA TACCCGACCT 
AAAGAAAAGA GGTTAGTGAA TGATGATTTA ATGCAGACAA GTTTTGAACG AATGCTAAGC 
CTTAAAGACC TAGAAATAGA ACAGTGGCAC AAAAAACATA AGGAAAAAAT TAAGCAAAAA 
GAAAAGGAAC GGTTGAGAAA CCGAAACTGT TTAGAACTTA AAATAAAAGA TAAAGAAAAA 
ACAAAGCATA CACCAACTGA ATCCAAAAAT AAAGAACTTA CTAGGTCAAA GAGTTCAGAA 
GTGACTGATG CATATACCAA GGAGAAACAA CCTAAAGATG CTGTAAGTAA CAGATCACAA 
TCTGTTGACA CCAAAAATGT AATGACTTTA GGGAAGTCAT CTTTTGTTTC AGATAATAGC 
TTAAACAGGT CTCCTAGATC AGAAAATGAA AAGCCGGGTC TCAGCTCCAG ATCTGTATCC 
ATGATTTCTG TTGCTAGTTC AGAAGATTCC TGCCATACTA CAGTGACAAC CCCAAGGCCT 
CCAGTTGAGT ATGACTCTGA CTTTATGTTA GAGAGTTCAG AATCCCAAAT GTCCTTTTCC 
CAGTCACCTT TTTTGTCAAT TGCCAAATCT CCTGCTCTTC ATGAAAGGGA ATTGGACAGC 
CTGGCTGACT TGCCGGAGCG GATTAAACCA CCATATGCAA ACAGACTTTC AACATCCCAT 
CTTAGGTCAT CTTCTGTAGA AGATGTTAAA CTAATTATAA GCGAGGGGAG ACCTACCATA 
GAAGTTCGAA GATGTAGCAT GCCTTCTGTC ATTTGTGAAC ATACCAAACA ATTCCAAACA 
ATATCAGAAG AGAGCAATCA AGGTAGCTTA TTAACTGTGC CAGGAGATAC TAGTCCTTCT 
CCCAAACCTG AGGTATTCTC AAATGTGCCT GAAAGAGACC TTTCAAATGT ATCTAACATA 
CATTCCAGTT TTGCAACTTC TCCAACTGGA GCTTCAAACA GCAAGTATGT TTCAGCTGAT 
AGAAATCTCA TCAAGAATAC TGCCCCAGTG AACACTGTAA TGGACAGTCC AGTGCATTTA 
GAGCCATCTA GTCAGGTTGG TGTGATCCAG AATAAATCAT GGGAGATGCC TGTTGATAGA 
CTAGAGACAT TAAGCACCAG AGACTTTATC TGCCCAAATT CTAACATACC TGATCAAGAA 
TCCTCTCTTC AGAGTTTTTG TAATTCTGAA AATAAGGTAT TGAAAGAAAA TGCTGATTTT 
TTATCCCTGC GCCAGACTGA ACTGCCAGGA AACTCTTGTG CTCAGGATCC GGCATCCTTT 
ATGCCTCCAC AGCAGCCTTG CTCTTTCCCC AGCCAATCAC TTTCAGATGC TGAATCGATT 
TCTAAACATA TGTCTTTGTC ATATGTTGCT AATCAAGAGC CAGGTATTTT ACAACAAAAA 
AATGCAGTTC AGATTATTAG TTCTGCTTTA GATACTGATA ATGAATCTAC AAAAGATACA 
GAAAATACTT TTGTCCTAGG AGATGTTCAA AAAACAGATG CCTTTGTCCC AGTGTACTCT 
GACAGCACTA TTCAAGAAGC ATCACCAAAC TTTGAGAAAG CTTATACTTT ACCTGTGTTA 
CCATCAGAAA AGGACTTTAA TGGAAGTGAT GCCTCTACCC AGCTAAATAC ACATTATGCA 
TTTAGCAAAC TAACTTACAA GTCTTCCAGT GGCCATGAAG TTGAGAATAG CACAACTGAT 
ACTCAGGTCA TTTCACATGA AAAAGAAAAC AAACTGGAGA GTTTGGTTTT AACTCATTTG 
AGTAGGTGTG ATTCTGATTT ATGTGAAATG AATGCAGGGA TGCCAAAAGG AAACCTAAAT 
GAACAAGATC CAAAACATTG TCCTGAAAGT GAAAAGTGTT TGCTTTCCAT AGAAGATGAG 
GAATCTCAAC AAAGCATTTT ATCAAGTCTG GAAAACCATT CACAGCAGTC AACTCAACCA 
GAAATGCATA AATATGGTCA GTTAGTTAAA GTAGAATTAG AAGAAAATGC CGAAGATGAT 
AAAACTGAAA ACCAAATCCC TCAAAGAATG ACTAGAAACA AAGCAAATAC AATGGCAAAT 
CAAAGCAAAC AGATTCTTGC TAGCTGTACA CTATTATCAG AAAAAGACAG TGAATCCTCA 
TCTCCTAGAG GAAGAATAAG ATTAACTGAA GATGACGATC CTCAAATTCA CCATCCACGG 
AAAAGGAAAG TGTCACGTGT ACCTCAGCCT GTGCAAGTGA GTCCCTCTTT ACTACAAGCA 
AAAGAGAAAA CTCAGCAATC TCTGGCAGCC ATTGTAGATT CTCTAAAACT AGATGAGATT 
CAGCCATACA GTTCAGAGAG AGCAAATCCA TATTTTGAAT ACTTGCACAT AAGGAAAAAA 
ATAGAAGAAA AACGCAAATT ACTGTGTAGT GTGATTCCTC AAGCACCTCA GTACTATGAC 
GAATATGTAA CATTTAACGG ATCATATCTC CTGGATGGAA ACCCCTTAAG CAAGATTTGT 
ATTCCCACAA TTACACCACC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TAAGGATGAA ACTACGTTTG CAACACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAACG AACAAGAAGT TTTGCGAGTT CATTACAGAG CTGCAAGAAC ATTGGCAAAT 
CAAACACTGC CATTTAGTGC TTGTACTGTT TTGCTGGATG CCGAAGTATA CAATGTACCA 
TTGGACTCTC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAA 
TTCATGTCTT GGTTACAAGA TGTGGATGAT AAATTTGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AACATGAAGC TGCGGCTTTA AATGCTGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATTTACGA AATCCAGGAG 
TTTTATGTTC CCCTTGTTGA TGTTAACGAC GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881155.1
CDS	323..6436
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNNSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025666.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025666.2

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAATTC CACACCAAAT CATCCATCAC AAACAACGCC TGCCCAAAAG 
AAAACTCCCA GTTCTTCATC TCGACAGAAA GATAAAGTTA ATAAAAGAAA TGAACGTGGT 
GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA AACAAGTTAA AGAATTAATA 
AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT GGACACCACT GCATGAAGCT 
TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG CAGCTGGAGC AGATGTTAAC 
ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG CTAGTAGTGG GCACAGAGAT 
ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC AAGCTAATAA ACATGGGGAG 
CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC TACTAAAAAG AGAGGTGCCT 
TTATCTGATG ATGATGAAAG TTACACAGAT TCCGAAGAGG CTCAATCTGT AAATCCTTCT 
AGTGTTGATG AAAATATTGA CTCTGAAACA GAGAAAGACT CTCTCATCTG TGAAAGTAAA 
CAGATACTTC CCAGTAAAAC ACCTCTTCCA TCTGCCCTTG ATGAGTATGA GTTCAAAGAT 
GATGATGATG AAGAAATTAA TAAGATGATT GATGATAGGC ATATTCTTAG GAAAGAACAA 
CGAAAAGAAA ATGAACCTGA AGCAGAAAAA ACTCATTTAT TTGCAAAACA GGAGAAAGCC 
TTCTATCCTA AATCATTTAA AAGTAAAAAA CAAAAGCCAT CTAGGGTCTT ATATTCAAGT 
ACTGAAAGTT CTGATGAAGA AGCTCTTCAG AATAAAAAGA TTTCTACTTC ATGTTCCGTC 
ATCCCTGAAA CATCAAATTC TGATATGCAA ACCAAAAAGG AATATGTAGT TTCAGGTGAA 
CACAAACAGA AAGGCAAAGT TAAAAGAAAA TTGAAAAATC AGAATAAAAA TAAAGAGAAC 
CAAGAGCTAA AGCAAGAAAA GGAAGGAAAA GAAAATACAA GAATAACAAA CTTGACAGTA 
AATACTGGAC TAGATTGTTC AGAAAAGACC AGAGAGGAGG GGAACTTTAG GAAATCTTTT 
AGCCCAAAAG ATGATACTTC ATTACATTTA TTTCATATTT CCACTGGTAA ATCTCCCAAA 
CATTCTTGTG GATTAAGTGA AAAACAGTCA ACACCACTAA AACAAGAACA TACTAAAACA 
TGTTTATCAC CAGGAAGTTC TGAAATGTCA TTACAGCCTG ATCTTGTTCG GTATGATAAT 
ACAGAATCTG AATTCTTGCC AGAAAGTTCA AGTGTAAAAT CTTGTAAGCA TAAGGAAAAA 
AGCAAACATC AGAAAGATTT CCACTTAGAA TTTGGTGAAA AATCAAATGC CAAAATAAAG 
GATGAAGATC ATAGTCCAAC ATTTGAAAAT TCAGATTGCA CACTGAAAAA AATGGATAAA 
GAAGGTAAAA CATTAAAAAA ACATAAATTG AAGCATAAAG AGAGGGAAAA AGAAAAGCAT 
AAAAAAGAAA TTGAAGGTGA AAAGGAAAAA TACAAAACTA AGGATAGTGC CAAAGAACTG 
CAGAGGAGTG TGGAATTTGA TAGAGAATTT TGGAAAGAGA ATTTTTTTAA AAGTGATGAA 
ACTGAAGATC TCTTTTTAAA TATGGAACAT GAATCCTTAA CATTAGAAAA AAAATCAAAA 
TTGGAAAAAA ACATCAAAGA TGATAAATCA ACCAAGGAAA AGCATGTGTC AAAAGAGAGG 
AACTTTAAAG AGGAACGAGA CAAGATTAAA AAGGAAAGCG AGAAATCTTT TAGGGAGGAA 
AAAATAAAAG ATCTAAAAGA AGAGAGAGAA AACATACCCA CAGATAAAGA CTCAGAATTT 
ACTTCTTTGG GTATGAGTGC CATTGAGGAA TCTATAGGGC TTCATTTAGT GGAAAAGGAA 
ATAGACATTG AAAAACAAGA AAAGCATATA AAGGAAAGTA AAGAAAAACC TGAGAAGCGA 
TCTCAAATTA AAGAAAAGGA CATTGAGAAG ATGGAAAGAA AAACCTTTGA AAAAGAAAAG 
AAGATAAAAC ATGAGCATAA GTCAGAAAAA GACAAATTAG ATCTTAGTGA ATGTGTTGAT 
AAAATAAAAG AAAAGGACAA GCTATATTCG CATCACACAG AAAAATGCCA TAAAGAAGGT 
GAGAAGAGCA AAAATACTGC TGCTATTAAA AAAACTGACG ACAGAGAGAA AAGTAGAGAA 
AAGATGGATA GGAAACATGA CAAAGAAAAG CCTGAAAAAG AGAGGCATCT AGCAGAAAGC 
AAAGAAAAGC ACTTGATGGA GAAAAAAAAT AAACAATCAG ATAATAGTGA ATACAGTAAA 
TCAGAAAAAG GCAAAAATAA AGAAAAAGAC AGGGAGCTAG ATAAAAAGGA AAAATCTAGA 
GATAAAGAAA GTATAAATAT AACTAACTCC AAACACATAC AGGAAGAAAA AAAATCAAGT 
ATAGTAGACG GTAATAAAGC ACAACATGAA AAACCCTTAT CCCTTAAAGA AAAAACAAAA 
GATGAACCTT TGAAAACTCC AGATGGAAAA GAAAAAGATA AAAAAGATAA AGATATAGAT 
AGATACAAAG AACGAGACAA ACATAAAGAT AAAATTCAAA TAAATAGCTT ACTCAAACTA 
AAATCTGAAG CAGATAAGCC TAAACCTAAG TCATCACCAG CATCAAAAGA TACCCGACCT 
AAAGAAAAGA GGTTAGTGAA TGATGATTTA ATGCAGACAA GTTTTGAACG AATGCTAAGC 
CTTAAAGACC TAGAAATAGA ACAGTGGCAC AAAAAACATA AGGAAAAAAT TAAGCAAAAA 
GAAAAGGAAC GGTTGAGAAA CCGAAACTGT TTAGAACTTA AAATAAAAGA TAAAGAAAAA 
ACAAAGCATA CACCAACTGA ATCCAAAAAT AAAGAACTTA CTAGGTCAAA GAGTTCAGAA 
GTGACTGATG CATATACCAA GGAGAAACAA CCTAAAGATG CTGTAAGTAA CAGATCACAA 
TCTGTTGACA CCAAAAATGT AATGACTTTA GGGAAGTCAT CTTTTGTTTC AGATAATAGC 
TTAAACAGGT CTCCTAGATC AGAAAATGAA AAGCCGGGTC TCAGCTCCAG ATCTGTATCC 
ATGATTTCTG TTGCTAGTTC AGAAGATTCC TGCCATACTA CAGTGACAAC CCCAAGGCCT 
CCAGTTGAGT ATGACTCTGA CTTTATGTTA GAGAGTTCAG AATCCCAAAT GTCCTTTTCC 
CAGTCACCTT TTTTGTCAAT TGCCAAATCT CCTGCTCTTC ATGAAAGGGA ATTGGACAGC 
CTGGCTGACT TGCCGGAGCG GATTAAACCA CCATATGCAA ACAGACTTTC AACATCCCAT 
CTTAGGTCAT CTTCTGTAGA AGATGTTAAA CTAATTATAA GCGAGGGGAG ACCTACCATA 
GAAGTTCGAA GATGTAGCAT GCCTTCTGTC ATTTGTGAAC ATACCAAACA ATTCCAAACA 
ATATCAGAAG AGAGCAATCA AGGTAGCTTA TTAACTGTGC CAGGAGATAC TAGTCCTTCT 
CCCAAACCTG AGGTATTCTC AAATGTGCCT GAAAGAGACC TTTCAAATGT ATCTAACATA 
CATTCCAGTT TTGCAACTTC TCCAACTGGA GCTTCAAACA GCAAGTATGT TTCAGCTGAT 
AGAAATCTCA TCAAGAATAC TGCCCCAGTG AACACTGTAA TGGACAGTCC AGTGCATTTA 
GAGCCATCTA GTCAGGTTGG TGTGATCCAG AATAAATCAT GGGAGATGCC TGTTGATAGA 
CTAGAGACAT TAAGCACCAG AGACTTTATC TGCCCAAATT CTAACATACC TGATCAAGAA 
TCCTCTCTTC AGAGTTTTTG TAATTCTGAA AATAAGGTAT TGAAAGAAAA TGCTGATTTT 
TTATCCCTGC GCCAGACTGA ACTGCCAGGA AACTCTTGTG CTCAGGATCC GGCATCCTTT 
ATGCCTCCAC AGCAGCCTTG CTCTTTCCCC AGCCAATCAC TTTCAGATGC TGAATCGATT 
TCTAAACATA TGTCTTTGTC ATATGTTGCT AATCAAGAGC CAGGTATTTT ACAACAAAAA 
AATGCAGTTC AGATTATTAG TTCTGCTTTA GATACTGATA ATGAATCTAC AAAAGATACA 
GAAAATACTT TTGTCCTAGG AGATGTTCAA AAAACAGATG CCTTTGTCCC AGTGTACTCT 
GACAGCACTA TTCAAGAAGC ATCACCAAAC TTTGAGAAAG CTTATACTTT ACCTGTGTTA 
CCATCAGAAA AGGACTTTAA TGGAAGTGAT GCCTCTACCC AGCTAAATAC ACATTATGCA 
TTTAGCAAAC TAACTTACAA GTCTTCCAGT GGCCATGAAG TTGAGAATAG CACAACTGAT 
ACTCAGGTCA TTTCACATGA AAAAGAAAAC AAACTGGAGA GTTTGGTTTT AACTCATTTG 
AGTAGGTGTG ATTCTGATTT ATGTGAAATG AATGCAGGGA TGCCAAAAGG AAACCTAAAT 
GAACAAGATC CAAAACATTG TCCTGAAAGT GAAAAGTGTT TGCTTTCCAT AGAAGATGAG 
GAATCTCAAC AAAGCATTTT ATCAAGTCTG GAAAACCATT CACAGCAGTC AACTCAACCA 
GAAATGCATA AATATGGTCA GTTAGTTAAA GTAGAATTAG AAGAAAATGC CGAAGATGAT 
AAAACTGAAA ACCAAATCCC TCAAAGAATG ACTAGAAACA AAGCAAATAC AATGGCAAAT 
CAAAGCAAAC AGATTCTTGC TAGCTGTACA CTATTATCAG AAAAAGACAG TGAATCCTCA 
TCTCCTAGAG GAAGAATAAG ATTAACTGAA GATGACGATC CTCAAATTCA CCATCCACGG 
AAAAGGAAAG TGTCACGTGT ACCTCAGCCT GTGCAAGTGA GTCCCTCTTT ACTACAAGCA 
AAAGAGAAAA CTCAGCAATC TCTGGCAGCC ATTGTAGATT CTCTAAAACT AGATGAGATT 
CAGCCATACA GTTCAGAGAG AGCAAATCCA TATTTTGAAT ACTTGCACAT AAGGAAAAAA 
ATAGAAGAAA AACGCAAATT ACTGTGTAGT GTGATTCCTC AAGCACCTCA GTACTATGAC 
GAATATGTAA CATTTAACGG ATCATATCTC CTGGATGGAA ACCCCTTAAG CAAGATTTGT 
ATTCCCACAA TTACACCACC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TAAGGATGAA ACTACGTTTG CAACACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAACG AACAAGAAGT TTTGCGAGTT CATTACAGAG CTGCAAGAAC ATTGGCAAAT 
CAAACACTGC CATTTAGTGC TTGTACTGTT TTGCTGGATG CCGAAGTATA CAATGTACCA 
TTGGACTCTC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAA 
TTCATGTCTT GGTTACAAGA TGTGGATGAT AAATTTGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AACATGAAGC TGCGGCTTTA AATGCTGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATTTACGA AATCCAGGAG 
TTTTATGTTC CCCTTGTTGA TGTTAACGAC GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025664.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881153.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X8, mRNA.

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CloneID	OHu50997
Clone ID Related Accession (Same CDS sequence)	XM_017025666.1 , XM_017025664.2 , XM_017025665.2 , XM_017025666.2 , XM_017025664.1 , XM_005258096.3 , XM_005258096.2 , XM_017025665.1
Accession Version	XM_017025664.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAATTC CACACCAAAT CATCCATCAC AAACAACGCC TGCCCAAAAG 
AAAACTCCCA GTTCTTCATC TCGACAGAAA GATAAAGTTA ATAAAAGAAA TGAACGTGGT 
GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA AACAAGTTAA AGAATTAATA 
AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT GGACACCACT GCATGAAGCT 
TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG CAGCTGGAGC AGATGTTAAC 
ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG CTAGTAGTGG GCACAGAGAT 
ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC AAGCTAATAA ACATGGGGAG 
CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC TACTAAAAAG AGAGGTGCCT 
TTATCTGATG ATGATGAAAG TTACACAGAT TCCGAAGAGG CTCAATCTGT AAATCCTTCT 
AGTGTTGATG AAAATATTGA CTCTGAAACA GAGAAAGACT CTCTCATCTG TGAAAGTAAA 
CAGATACTTC CCAGTAAAAC ACCTCTTCCA TCTGCCCTTG ATGAGTATGA GTTCAAAGAT 
GATGATGATG AAGAAATTAA TAAGATGATT GATGATAGGC ATATTCTTAG GAAAGAACAA 
CGAAAAGAAA ATGAACCTGA AGCAGAAAAA ACTCATTTAT TTGCAAAACA GGAGAAAGCC 
TTCTATCCTA AATCATTTAA AAGTAAAAAA CAAAAGCCAT CTAGGGTCTT ATATTCAAGT 
ACTGAAAGTT CTGATGAAGA AGCTCTTCAG AATAAAAAGA TTTCTACTTC ATGTTCCGTC 
ATCCCTGAAA CATCAAATTC TGATATGCAA ACCAAAAAGG AATATGTAGT TTCAGGTGAA 
CACAAACAGA AAGGCAAAGT TAAAAGAAAA TTGAAAAATC AGAATAAAAA TAAAGAGAAC 
CAAGAGCTAA AGCAAGAAAA GGAAGGAAAA GAAAATACAA GAATAACAAA CTTGACAGTA 
AATACTGGAC TAGATTGTTC AGAAAAGACC AGAGAGGAGG GGAACTTTAG GAAATCTTTT 
AGCCCAAAAG ATGATACTTC ATTACATTTA TTTCATATTT CCACTGGTAA ATCTCCCAAA 
CATTCTTGTG GATTAAGTGA AAAACAGTCA ACACCACTAA AACAAGAACA TACTAAAACA 
TGTTTATCAC CAGGAAGTTC TGAAATGTCA TTACAGCCTG ATCTTGTTCG GTATGATAAT 
ACAGAATCTG AATTCTTGCC AGAAAGTTCA AGTGTAAAAT CTTGTAAGCA TAAGGAAAAA 
AGCAAACATC AGAAAGATTT CCACTTAGAA TTTGGTGAAA AATCAAATGC CAAAATAAAG 
GATGAAGATC ATAGTCCAAC ATTTGAAAAT TCAGATTGCA CACTGAAAAA AATGGATAAA 
GAAGGTAAAA CATTAAAAAA ACATAAATTG AAGCATAAAG AGAGGGAAAA AGAAAAGCAT 
AAAAAAGAAA TTGAAGGTGA AAAGGAAAAA TACAAAACTA AGGATAGTGC CAAAGAACTG 
CAGAGGAGTG TGGAATTTGA TAGAGAATTT TGGAAAGAGA ATTTTTTTAA AAGTGATGAA 
ACTGAAGATC TCTTTTTAAA TATGGAACAT GAATCCTTAA CATTAGAAAA AAAATCAAAA 
TTGGAAAAAA ACATCAAAGA TGATAAATCA ACCAAGGAAA AGCATGTGTC AAAAGAGAGG 
AACTTTAAAG AGGAACGAGA CAAGATTAAA AAGGAAAGCG AGAAATCTTT TAGGGAGGAA 
AAAATAAAAG ATCTAAAAGA AGAGAGAGAA AACATACCCA CAGATAAAGA CTCAGAATTT 
ACTTCTTTGG GTATGAGTGC CATTGAGGAA TCTATAGGGC TTCATTTAGT GGAAAAGGAA 
ATAGACATTG AAAAACAAGA AAAGCATATA AAGGAAAGTA AAGAAAAACC TGAGAAGCGA 
TCTCAAATTA AAGAAAAGGA CATTGAGAAG ATGGAAAGAA AAACCTTTGA AAAAGAAAAG 
AAGATAAAAC ATGAGCATAA GTCAGAAAAA GACAAATTAG ATCTTAGTGA ATGTGTTGAT 
AAAATAAAAG AAAAGGACAA GCTATATTCG CATCACACAG AAAAATGCCA TAAAGAAGGT 
GAGAAGAGCA AAAATACTGC TGCTATTAAA AAAACTGACG ACAGAGAGAA AAGTAGAGAA 
AAGATGGATA GGAAACATGA CAAAGAAAAG CCTGAAAAAG AGAGGCATCT AGCAGAAAGC 
AAAGAAAAGC ACTTGATGGA GAAAAAAAAT AAACAATCAG ATAATAGTGA ATACAGTAAA 
TCAGAAAAAG GCAAAAATAA AGAAAAAGAC AGGGAGCTAG ATAAAAAGGA AAAATCTAGA 
GATAAAGAAA GTATAAATAT AACTAACTCC AAACACATAC AGGAAGAAAA AAAATCAAGT 
ATAGTAGACG GTAATAAAGC ACAACATGAA AAACCCTTAT CCCTTAAAGA AAAAACAAAA 
GATGAACCTT TGAAAACTCC AGATGGAAAA GAAAAAGATA AAAAAGATAA AGATATAGAT 
AGATACAAAG AACGAGACAA ACATAAAGAT AAAATTCAAA TAAATAGCTT ACTCAAACTA 
AAATCTGAAG CAGATAAGCC TAAACCTAAG TCATCACCAG CATCAAAAGA TACCCGACCT 
AAAGAAAAGA GGTTAGTGAA TGATGATTTA ATGCAGACAA GTTTTGAACG AATGCTAAGC 
CTTAAAGACC TAGAAATAGA ACAGTGGCAC AAAAAACATA AGGAAAAAAT TAAGCAAAAA 
GAAAAGGAAC GGTTGAGAAA CCGAAACTGT TTAGAACTTA AAATAAAAGA TAAAGAAAAA 
ACAAAGCATA CACCAACTGA ATCCAAAAAT AAAGAACTTA CTAGGTCAAA GAGTTCAGAA 
GTGACTGATG CATATACCAA GGAGAAACAA CCTAAAGATG CTGTAAGTAA CAGATCACAA 
TCTGTTGACA CCAAAAATGT AATGACTTTA GGGAAGTCAT CTTTTGTTTC AGATAATAGC 
TTAAACAGGT CTCCTAGATC AGAAAATGAA AAGCCGGGTC TCAGCTCCAG ATCTGTATCC 
ATGATTTCTG TTGCTAGTTC AGAAGATTCC TGCCATACTA CAGTGACAAC CCCAAGGCCT 
CCAGTTGAGT ATGACTCTGA CTTTATGTTA GAGAGTTCAG AATCCCAAAT GTCCTTTTCC 
CAGTCACCTT TTTTGTCAAT TGCCAAATCT CCTGCTCTTC ATGAAAGGGA ATTGGACAGC 
CTGGCTGACT TGCCGGAGCG GATTAAACCA CCATATGCAA ACAGACTTTC AACATCCCAT 
CTTAGGTCAT CTTCTGTAGA AGATGTTAAA CTAATTATAA GCGAGGGGAG ACCTACCATA 
GAAGTTCGAA GATGTAGCAT GCCTTCTGTC ATTTGTGAAC ATACCAAACA ATTCCAAACA 
ATATCAGAAG AGAGCAATCA AGGTAGCTTA TTAACTGTGC CAGGAGATAC TAGTCCTTCT 
CCCAAACCTG AGGTATTCTC AAATGTGCCT GAAAGAGACC TTTCAAATGT ATCTAACATA 
CATTCCAGTT TTGCAACTTC TCCAACTGGA GCTTCAAACA GCAAGTATGT TTCAGCTGAT 
AGAAATCTCA TCAAGAATAC TGCCCCAGTG AACACTGTAA TGGACAGTCC AGTGCATTTA 
GAGCCATCTA GTCAGGTTGG TGTGATCCAG AATAAATCAT GGGAGATGCC TGTTGATAGA 
CTAGAGACAT TAAGCACCAG AGACTTTATC TGCCCAAATT CTAACATACC TGATCAAGAA 
TCCTCTCTTC AGAGTTTTTG TAATTCTGAA AATAAGGTAT TGAAAGAAAA TGCTGATTTT 
TTATCCCTGC GCCAGACTGA ACTGCCAGGA AACTCTTGTG CTCAGGATCC GGCATCCTTT 
ATGCCTCCAC AGCAGCCTTG CTCTTTCCCC AGCCAATCAC TTTCAGATGC TGAATCGATT 
TCTAAACATA TGTCTTTGTC ATATGTTGCT AATCAAGAGC CAGGTATTTT ACAACAAAAA 
AATGCAGTTC AGATTATTAG TTCTGCTTTA GATACTGATA ATGAATCTAC AAAAGATACA 
GAAAATACTT TTGTCCTAGG AGATGTTCAA AAAACAGATG CCTTTGTCCC AGTGTACTCT 
GACAGCACTA TTCAAGAAGC ATCACCAAAC TTTGAGAAAG CTTATACTTT ACCTGTGTTA 
CCATCAGAAA AGGACTTTAA TGGAAGTGAT GCCTCTACCC AGCTAAATAC ACATTATGCA 
TTTAGCAAAC TAACTTACAA GTCTTCCAGT GGCCATGAAG TTGAGAATAG CACAACTGAT 
ACTCAGGTCA TTTCACATGA AAAAGAAAAC AAACTGGAGA GTTTGGTTTT AACTCATTTG 
AGTAGGTGTG ATTCTGATTT ATGTGAAATG AATGCAGGGA TGCCAAAAGG AAACCTAAAT 
GAACAAGATC CAAAACATTG TCCTGAAAGT GAAAAGTGTT TGCTTTCCAT AGAAGATGAG 
GAATCTCAAC AAAGCATTTT ATCAAGTCTG GAAAACCATT CACAGCAGTC AACTCAACCA 
GAAATGCATA AATATGGTCA GTTAGTTAAA GTAGAATTAG AAGAAAATGC CGAAGATGAT 
AAAACTGAAA ACCAAATCCC TCAAAGAATG ACTAGAAACA AAGCAAATAC AATGGCAAAT 
CAAAGCAAAC AGATTCTTGC TAGCTGTACA CTATTATCAG AAAAAGACAG TGAATCCTCA 
TCTCCTAGAG GAAGAATAAG ATTAACTGAA GATGACGATC CTCAAATTCA CCATCCACGG 
AAAAGGAAAG TGTCACGTGT ACCTCAGCCT GTGCAAGTGA GTCCCTCTTT ACTACAAGCA 
AAAGAGAAAA CTCAGCAATC TCTGGCAGCC ATTGTAGATT CTCTAAAACT AGATGAGATT 
CAGCCATACA GTTCAGAGAG AGCAAATCCA TATTTTGAAT ACTTGCACAT AAGGAAAAAA 
ATAGAAGAAA AACGCAAATT ACTGTGTAGT GTGATTCCTC AAGCACCTCA GTACTATGAC 
GAATATGTAA CATTTAACGG ATCATATCTC CTGGATGGAA ACCCCTTAAG CAAGATTTGT 
ATTCCCACAA TTACACCACC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TAAGGATGAA ACTACGTTTG CAACACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAACG AACAAGAAGT TTTGCGAGTT CATTACAGAG CTGCAAGAAC ATTGGCAAAT 
CAAACACTGC CATTTAGTGC TTGTACTGTT TTGCTGGATG CCGAAGTATA CAATGTACCA 
TTGGACTCTC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAA 
TTCATGTCTT GGTTACAAGA TGTGGATGAT AAATTTGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AACATGAAGC TGCGGCTTTA AATGCTGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATTTACGA AATCCAGGAG 
TTTTATGTTC CCCTTGTTGA TGTTAACGAC GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881153.1
CDS	350..6463
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNNSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025664.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025664.1

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAATTC CACACCAAAT CATCCATCAC AAACAACGCC TGCCCAAAAG 
AAAACTCCCA GTTCTTCATC TCGACAGAAA GATAAAGTTA ATAAAAGAAA TGAACGTGGT 
GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA AACAAGTTAA AGAATTAATA 
AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT GGACACCACT GCATGAAGCT 
TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG CAGCTGGAGC AGATGTTAAC 
ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG CTAGTAGTGG GCACAGAGAT 
ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC AAGCTAATAA ACATGGGGAG 
CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC TACTAAAAAG AGAGGTGCCT 
TTATCTGATG ATGATGAAAG TTACACAGAT TCCGAAGAGG CTCAATCTGT AAATCCTTCT 
AGTGTTGATG AAAATATTGA CTCTGAAACA GAGAAAGACT CTCTCATCTG TGAAAGTAAA 
CAGATACTTC CCAGTAAAAC ACCTCTTCCA TCTGCCCTTG ATGAGTATGA GTTCAAAGAT 
GATGATGATG AAGAAATTAA TAAGATGATT GATGATAGGC ATATTCTTAG GAAAGAACAA 
CGAAAAGAAA ATGAACCTGA AGCAGAAAAA ACTCATTTAT TTGCAAAACA GGAGAAAGCC 
TTCTATCCTA AATCATTTAA AAGTAAAAAA CAAAAGCCAT CTAGGGTCTT ATATTCAAGT 
ACTGAAAGTT CTGATGAAGA AGCTCTTCAG AATAAAAAGA TTTCTACTTC ATGTTCCGTC 
ATCCCTGAAA CATCAAATTC TGATATGCAA ACCAAAAAGG AATATGTAGT TTCAGGTGAA 
CACAAACAGA AAGGCAAAGT TAAAAGAAAA TTGAAAAATC AGAATAAAAA TAAAGAGAAC 
CAAGAGCTAA AGCAAGAAAA GGAAGGAAAA GAAAATACAA GAATAACAAA CTTGACAGTA 
AATACTGGAC TAGATTGTTC AGAAAAGACC AGAGAGGAGG GGAACTTTAG GAAATCTTTT 
AGCCCAAAAG ATGATACTTC ATTACATTTA TTTCATATTT CCACTGGTAA ATCTCCCAAA 
CATTCTTGTG GATTAAGTGA AAAACAGTCA ACACCACTAA AACAAGAACA TACTAAAACA 
TGTTTATCAC CAGGAAGTTC TGAAATGTCA TTACAGCCTG ATCTTGTTCG GTATGATAAT 
ACAGAATCTG AATTCTTGCC AGAAAGTTCA AGTGTAAAAT CTTGTAAGCA TAAGGAAAAA 
AGCAAACATC AGAAAGATTT CCACTTAGAA TTTGGTGAAA AATCAAATGC CAAAATAAAG 
GATGAAGATC ATAGTCCAAC ATTTGAAAAT TCAGATTGCA CACTGAAAAA AATGGATAAA 
GAAGGTAAAA CATTAAAAAA ACATAAATTG AAGCATAAAG AGAGGGAAAA AGAAAAGCAT 
AAAAAAGAAA TTGAAGGTGA AAAGGAAAAA TACAAAACTA AGGATAGTGC CAAAGAACTG 
CAGAGGAGTG TGGAATTTGA TAGAGAATTT TGGAAAGAGA ATTTTTTTAA AAGTGATGAA 
ACTGAAGATC TCTTTTTAAA TATGGAACAT GAATCCTTAA CATTAGAAAA AAAATCAAAA 
TTGGAAAAAA ACATCAAAGA TGATAAATCA ACCAAGGAAA AGCATGTGTC AAAAGAGAGG 
AACTTTAAAG AGGAACGAGA CAAGATTAAA AAGGAAAGCG AGAAATCTTT TAGGGAGGAA 
AAAATAAAAG ATCTAAAAGA AGAGAGAGAA AACATACCCA CAGATAAAGA CTCAGAATTT 
ACTTCTTTGG GTATGAGTGC CATTGAGGAA TCTATAGGGC TTCATTTAGT GGAAAAGGAA 
ATAGACATTG AAAAACAAGA AAAGCATATA AAGGAAAGTA AAGAAAAACC TGAGAAGCGA 
TCTCAAATTA AAGAAAAGGA CATTGAGAAG ATGGAAAGAA AAACCTTTGA AAAAGAAAAG 
AAGATAAAAC ATGAGCATAA GTCAGAAAAA GACAAATTAG ATCTTAGTGA ATGTGTTGAT 
AAAATAAAAG AAAAGGACAA GCTATATTCG CATCACACAG AAAAATGCCA TAAAGAAGGT 
GAGAAGAGCA AAAATACTGC TGCTATTAAA AAAACTGACG ACAGAGAGAA AAGTAGAGAA 
AAGATGGATA GGAAACATGA CAAAGAAAAG CCTGAAAAAG AGAGGCATCT AGCAGAAAGC 
AAAGAAAAGC ACTTGATGGA GAAAAAAAAT AAACAATCAG ATAATAGTGA ATACAGTAAA 
TCAGAAAAAG GCAAAAATAA AGAAAAAGAC AGGGAGCTAG ATAAAAAGGA AAAATCTAGA 
GATAAAGAAA GTATAAATAT AACTAACTCC AAACACATAC AGGAAGAAAA AAAATCAAGT 
ATAGTAGACG GTAATAAAGC ACAACATGAA AAACCCTTAT CCCTTAAAGA AAAAACAAAA 
GATGAACCTT TGAAAACTCC AGATGGAAAA GAAAAAGATA AAAAAGATAA AGATATAGAT 
AGATACAAAG AACGAGACAA ACATAAAGAT AAAATTCAAA TAAATAGCTT ACTCAAACTA 
AAATCTGAAG CAGATAAGCC TAAACCTAAG TCATCACCAG CATCAAAAGA TACCCGACCT 
AAAGAAAAGA GGTTAGTGAA TGATGATTTA ATGCAGACAA GTTTTGAACG AATGCTAAGC 
CTTAAAGACC TAGAAATAGA ACAGTGGCAC AAAAAACATA AGGAAAAAAT TAAGCAAAAA 
GAAAAGGAAC GGTTGAGAAA CCGAAACTGT TTAGAACTTA AAATAAAAGA TAAAGAAAAA 
ACAAAGCATA CACCAACTGA ATCCAAAAAT AAAGAACTTA CTAGGTCAAA GAGTTCAGAA 
GTGACTGATG CATATACCAA GGAGAAACAA CCTAAAGATG CTGTAAGTAA CAGATCACAA 
TCTGTTGACA CCAAAAATGT AATGACTTTA GGGAAGTCAT CTTTTGTTTC AGATAATAGC 
TTAAACAGGT CTCCTAGATC AGAAAATGAA AAGCCGGGTC TCAGCTCCAG ATCTGTATCC 
ATGATTTCTG TTGCTAGTTC AGAAGATTCC TGCCATACTA CAGTGACAAC CCCAAGGCCT 
CCAGTTGAGT ATGACTCTGA CTTTATGTTA GAGAGTTCAG AATCCCAAAT GTCCTTTTCC 
CAGTCACCTT TTTTGTCAAT TGCCAAATCT CCTGCTCTTC ATGAAAGGGA ATTGGACAGC 
CTGGCTGACT TGCCGGAGCG GATTAAACCA CCATATGCAA ACAGACTTTC AACATCCCAT 
CTTAGGTCAT CTTCTGTAGA AGATGTTAAA CTAATTATAA GCGAGGGGAG ACCTACCATA 
GAAGTTCGAA GATGTAGCAT GCCTTCTGTC ATTTGTGAAC ATACCAAACA ATTCCAAACA 
ATATCAGAAG AGAGCAATCA AGGTAGCTTA TTAACTGTGC CAGGAGATAC TAGTCCTTCT 
CCCAAACCTG AGGTATTCTC AAATGTGCCT GAAAGAGACC TTTCAAATGT ATCTAACATA 
CATTCCAGTT TTGCAACTTC TCCAACTGGA GCTTCAAACA GCAAGTATGT TTCAGCTGAT 
AGAAATCTCA TCAAGAATAC TGCCCCAGTG AACACTGTAA TGGACAGTCC AGTGCATTTA 
GAGCCATCTA GTCAGGTTGG TGTGATCCAG AATAAATCAT GGGAGATGCC TGTTGATAGA 
CTAGAGACAT TAAGCACCAG AGACTTTATC TGCCCAAATT CTAACATACC TGATCAAGAA 
TCCTCTCTTC AGAGTTTTTG TAATTCTGAA AATAAGGTAT TGAAAGAAAA TGCTGATTTT 
TTATCCCTGC GCCAGACTGA ACTGCCAGGA AACTCTTGTG CTCAGGATCC GGCATCCTTT 
ATGCCTCCAC AGCAGCCTTG CTCTTTCCCC AGCCAATCAC TTTCAGATGC TGAATCGATT 
TCTAAACATA TGTCTTTGTC ATATGTTGCT AATCAAGAGC CAGGTATTTT ACAACAAAAA 
AATGCAGTTC AGATTATTAG TTCTGCTTTA GATACTGATA ATGAATCTAC AAAAGATACA 
GAAAATACTT TTGTCCTAGG AGATGTTCAA AAAACAGATG CCTTTGTCCC AGTGTACTCT 
GACAGCACTA TTCAAGAAGC ATCACCAAAC TTTGAGAAAG CTTATACTTT ACCTGTGTTA 
CCATCAGAAA AGGACTTTAA TGGAAGTGAT GCCTCTACCC AGCTAAATAC ACATTATGCA 
TTTAGCAAAC TAACTTACAA GTCTTCCAGT GGCCATGAAG TTGAGAATAG CACAACTGAT 
ACTCAGGTCA TTTCACATGA AAAAGAAAAC AAACTGGAGA GTTTGGTTTT AACTCATTTG 
AGTAGGTGTG ATTCTGATTT ATGTGAAATG AATGCAGGGA TGCCAAAAGG AAACCTAAAT 
GAACAAGATC CAAAACATTG TCCTGAAAGT GAAAAGTGTT TGCTTTCCAT AGAAGATGAG 
GAATCTCAAC AAAGCATTTT ATCAAGTCTG GAAAACCATT CACAGCAGTC AACTCAACCA 
GAAATGCATA AATATGGTCA GTTAGTTAAA GTAGAATTAG AAGAAAATGC CGAAGATGAT 
AAAACTGAAA ACCAAATCCC TCAAAGAATG ACTAGAAACA AAGCAAATAC AATGGCAAAT 
CAAAGCAAAC AGATTCTTGC TAGCTGTACA CTATTATCAG AAAAAGACAG TGAATCCTCA 
TCTCCTAGAG GAAGAATAAG ATTAACTGAA GATGACGATC CTCAAATTCA CCATCCACGG 
AAAAGGAAAG TGTCACGTGT ACCTCAGCCT GTGCAAGTGA GTCCCTCTTT ACTACAAGCA 
AAAGAGAAAA CTCAGCAATC TCTGGCAGCC ATTGTAGATT CTCTAAAACT AGATGAGATT 
CAGCCATACA GTTCAGAGAG AGCAAATCCA TATTTTGAAT ACTTGCACAT AAGGAAAAAA 
ATAGAAGAAA AACGCAAATT ACTGTGTAGT GTGATTCCTC AAGCACCTCA GTACTATGAC 
GAATATGTAA CATTTAACGG ATCATATCTC CTGGATGGAA ACCCCTTAAG CAAGATTTGT 
ATTCCCACAA TTACACCACC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TAAGGATGAA ACTACGTTTG CAACACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAACG AACAAGAAGT TTTGCGAGTT CATTACAGAG CTGCAAGAAC ATTGGCAAAT 
CAAACACTGC CATTTAGTGC TTGTACTGTT TTGCTGGATG CCGAAGTATA CAATGTACCA 
TTGGACTCTC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAA 
TTCATGTCTT GGTTACAAGA TGTGGATGAT AAATTTGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AACATGAAGC TGCGGCTTTA AATGCTGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATTTACGA AATCCAGGAG 
TTTTATGTTC CCCTTGTTGA TGTTAACGAC GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_005258096.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258153.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X8, mRNA.

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CloneID	OHu50997
Clone ID Related Accession (Same CDS sequence)	XM_017025666.1 , XM_017025664.2 , XM_017025665.2 , XM_017025666.2 , XM_017025664.1 , XM_005258096.3 , XM_005258096.2 , XM_017025665.1
Accession Version	XM_005258096.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005258096.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAATTC CACACCAAAT CATCCATCAC AAACAACGCC TGCCCAAAAG 
AAAACTCCCA GTTCTTCATC TCGACAGAAA GATAAAGTTA ATAAAAGAAA TGAACGTGGT 
GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA AACAAGTTAA AGAATTAATA 
AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT GGACACCACT GCATGAAGCT 
TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG CAGCTGGAGC AGATGTTAAC 
ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG CTAGTAGTGG GCACAGAGAT 
ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC AAGCTAATAA ACATGGGGAG 
CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC TACTAAAAAG AGAGGTGCCT 
TTATCTGATG ATGATGAAAG TTACACAGAT TCCGAAGAGG CTCAATCTGT AAATCCTTCT 
AGTGTTGATG AAAATATTGA CTCTGAAACA GAGAAAGACT CTCTCATCTG TGAAAGTAAA 
CAGATACTTC CCAGTAAAAC ACCTCTTCCA TCTGCCCTTG ATGAGTATGA GTTCAAAGAT 
GATGATGATG AAGAAATTAA TAAGATGATT GATGATAGGC ATATTCTTAG GAAAGAACAA 
CGAAAAGAAA ATGAACCTGA AGCAGAAAAA ACTCATTTAT TTGCAAAACA GGAGAAAGCC 
TTCTATCCTA AATCATTTAA AAGTAAAAAA CAAAAGCCAT CTAGGGTCTT ATATTCAAGT 
ACTGAAAGTT CTGATGAAGA AGCTCTTCAG AATAAAAAGA TTTCTACTTC ATGTTCCGTC 
ATCCCTGAAA CATCAAATTC TGATATGCAA ACCAAAAAGG AATATGTAGT TTCAGGTGAA 
CACAAACAGA AAGGCAAAGT TAAAAGAAAA TTGAAAAATC AGAATAAAAA TAAAGAGAAC 
CAAGAGCTAA AGCAAGAAAA GGAAGGAAAA GAAAATACAA GAATAACAAA CTTGACAGTA 
AATACTGGAC TAGATTGTTC AGAAAAGACC AGAGAGGAGG GGAACTTTAG GAAATCTTTT 
AGCCCAAAAG ATGATACTTC ATTACATTTA TTTCATATTT CCACTGGTAA ATCTCCCAAA 
CATTCTTGTG GATTAAGTGA AAAACAGTCA ACACCACTAA AACAAGAACA TACTAAAACA 
TGTTTATCAC CAGGAAGTTC TGAAATGTCA TTACAGCCTG ATCTTGTTCG GTATGATAAT 
ACAGAATCTG AATTCTTGCC AGAAAGTTCA AGTGTAAAAT CTTGTAAGCA TAAGGAAAAA 
AGCAAACATC AGAAAGATTT CCACTTAGAA TTTGGTGAAA AATCAAATGC CAAAATAAAG 
GATGAAGATC ATAGTCCAAC ATTTGAAAAT TCAGATTGCA CACTGAAAAA AATGGATAAA 
GAAGGTAAAA CATTAAAAAA ACATAAATTG AAGCATAAAG AGAGGGAAAA AGAAAAGCAT 
AAAAAAGAAA TTGAAGGTGA AAAGGAAAAA TACAAAACTA AGGATAGTGC CAAAGAACTG 
CAGAGGAGTG TGGAATTTGA TAGAGAATTT TGGAAAGAGA ATTTTTTTAA AAGTGATGAA 
ACTGAAGATC TCTTTTTAAA TATGGAACAT GAATCCTTAA CATTAGAAAA AAAATCAAAA 
TTGGAAAAAA ACATCAAAGA TGATAAATCA ACCAAGGAAA AGCATGTGTC AAAAGAGAGG 
AACTTTAAAG AGGAACGAGA CAAGATTAAA AAGGAAAGCG AGAAATCTTT TAGGGAGGAA 
AAAATAAAAG ATCTAAAAGA AGAGAGAGAA AACATACCCA CAGATAAAGA CTCAGAATTT 
ACTTCTTTGG GTATGAGTGC CATTGAGGAA TCTATAGGGC TTCATTTAGT GGAAAAGGAA 
ATAGACATTG AAAAACAAGA AAAGCATATA AAGGAAAGTA AAGAAAAACC TGAGAAGCGA 
TCTCAAATTA AAGAAAAGGA CATTGAGAAG ATGGAAAGAA AAACCTTTGA AAAAGAAAAG 
AAGATAAAAC ATGAGCATAA GTCAGAAAAA GACAAATTAG ATCTTAGTGA ATGTGTTGAT 
AAAATAAAAG AAAAGGACAA GCTATATTCG CATCACACAG AAAAATGCCA TAAAGAAGGT 
GAGAAGAGCA AAAATACTGC TGCTATTAAA AAAACTGACG ACAGAGAGAA AAGTAGAGAA 
AAGATGGATA GGAAACATGA CAAAGAAAAG CCTGAAAAAG AGAGGCATCT AGCAGAAAGC 
AAAGAAAAGC ACTTGATGGA GAAAAAAAAT AAACAATCAG ATAATAGTGA ATACAGTAAA 
TCAGAAAAAG GCAAAAATAA AGAAAAAGAC AGGGAGCTAG ATAAAAAGGA AAAATCTAGA 
GATAAAGAAA GTATAAATAT AACTAACTCC AAACACATAC AGGAAGAAAA AAAATCAAGT 
ATAGTAGACG GTAATAAAGC ACAACATGAA AAACCCTTAT CCCTTAAAGA AAAAACAAAA 
GATGAACCTT TGAAAACTCC AGATGGAAAA GAAAAAGATA AAAAAGATAA AGATATAGAT 
AGATACAAAG AACGAGACAA ACATAAAGAT AAAATTCAAA TAAATAGCTT ACTCAAACTA 
AAATCTGAAG CAGATAAGCC TAAACCTAAG TCATCACCAG CATCAAAAGA TACCCGACCT 
AAAGAAAAGA GGTTAGTGAA TGATGATTTA ATGCAGACAA GTTTTGAACG AATGCTAAGC 
CTTAAAGACC TAGAAATAGA ACAGTGGCAC AAAAAACATA AGGAAAAAAT TAAGCAAAAA 
GAAAAGGAAC GGTTGAGAAA CCGAAACTGT TTAGAACTTA AAATAAAAGA TAAAGAAAAA 
ACAAAGCATA CACCAACTGA ATCCAAAAAT AAAGAACTTA CTAGGTCAAA GAGTTCAGAA 
GTGACTGATG CATATACCAA GGAGAAACAA CCTAAAGATG CTGTAAGTAA CAGATCACAA 
TCTGTTGACA CCAAAAATGT AATGACTTTA GGGAAGTCAT CTTTTGTTTC AGATAATAGC 
TTAAACAGGT CTCCTAGATC AGAAAATGAA AAGCCGGGTC TCAGCTCCAG ATCTGTATCC 
ATGATTTCTG TTGCTAGTTC AGAAGATTCC TGCCATACTA CAGTGACAAC CCCAAGGCCT 
CCAGTTGAGT ATGACTCTGA CTTTATGTTA GAGAGTTCAG AATCCCAAAT GTCCTTTTCC 
CAGTCACCTT TTTTGTCAAT TGCCAAATCT CCTGCTCTTC ATGAAAGGGA ATTGGACAGC 
CTGGCTGACT TGCCGGAGCG GATTAAACCA CCATATGCAA ACAGACTTTC AACATCCCAT 
CTTAGGTCAT CTTCTGTAGA AGATGTTAAA CTAATTATAA GCGAGGGGAG ACCTACCATA 
GAAGTTCGAA GATGTAGCAT GCCTTCTGTC ATTTGTGAAC ATACCAAACA ATTCCAAACA 
ATATCAGAAG AGAGCAATCA AGGTAGCTTA TTAACTGTGC CAGGAGATAC TAGTCCTTCT 
CCCAAACCTG AGGTATTCTC AAATGTGCCT GAAAGAGACC TTTCAAATGT ATCTAACATA 
CATTCCAGTT TTGCAACTTC TCCAACTGGA GCTTCAAACA GCAAGTATGT TTCAGCTGAT 
AGAAATCTCA TCAAGAATAC TGCCCCAGTG AACACTGTAA TGGACAGTCC AGTGCATTTA 
GAGCCATCTA GTCAGGTTGG TGTGATCCAG AATAAATCAT GGGAGATGCC TGTTGATAGA 
CTAGAGACAT TAAGCACCAG AGACTTTATC TGCCCAAATT CTAACATACC TGATCAAGAA 
TCCTCTCTTC AGAGTTTTTG TAATTCTGAA AATAAGGTAT TGAAAGAAAA TGCTGATTTT 
TTATCCCTGC GCCAGACTGA ACTGCCAGGA AACTCTTGTG CTCAGGATCC GGCATCCTTT 
ATGCCTCCAC AGCAGCCTTG CTCTTTCCCC AGCCAATCAC TTTCAGATGC TGAATCGATT 
TCTAAACATA TGTCTTTGTC ATATGTTGCT AATCAAGAGC CAGGTATTTT ACAACAAAAA 
AATGCAGTTC AGATTATTAG TTCTGCTTTA GATACTGATA ATGAATCTAC AAAAGATACA 
GAAAATACTT TTGTCCTAGG AGATGTTCAA AAAACAGATG CCTTTGTCCC AGTGTACTCT 
GACAGCACTA TTCAAGAAGC ATCACCAAAC TTTGAGAAAG CTTATACTTT ACCTGTGTTA 
CCATCAGAAA AGGACTTTAA TGGAAGTGAT GCCTCTACCC AGCTAAATAC ACATTATGCA 
TTTAGCAAAC TAACTTACAA GTCTTCCAGT GGCCATGAAG TTGAGAATAG CACAACTGAT 
ACTCAGGTCA TTTCACATGA AAAAGAAAAC AAACTGGAGA GTTTGGTTTT AACTCATTTG 
AGTAGGTGTG ATTCTGATTT ATGTGAAATG AATGCAGGGA TGCCAAAAGG AAACCTAAAT 
GAACAAGATC CAAAACATTG TCCTGAAAGT GAAAAGTGTT TGCTTTCCAT AGAAGATGAG 
GAATCTCAAC AAAGCATTTT ATCAAGTCTG GAAAACCATT CACAGCAGTC AACTCAACCA 
GAAATGCATA AATATGGTCA GTTAGTTAAA GTAGAATTAG AAGAAAATGC CGAAGATGAT 
AAAACTGAAA ACCAAATCCC TCAAAGAATG ACTAGAAACA AAGCAAATAC AATGGCAAAT 
CAAAGCAAAC AGATTCTTGC TAGCTGTACA CTATTATCAG AAAAAGACAG TGAATCCTCA 
TCTCCTAGAG GAAGAATAAG ATTAACTGAA GATGACGATC CTCAAATTCA CCATCCACGG 
AAAAGGAAAG TGTCACGTGT ACCTCAGCCT GTGCAAGTGA GTCCCTCTTT ACTACAAGCA 
AAAGAGAAAA CTCAGCAATC TCTGGCAGCC ATTGTAGATT CTCTAAAACT AGATGAGATT 
CAGCCATACA GTTCAGAGAG AGCAAATCCA TATTTTGAAT ACTTGCACAT AAGGAAAAAA 
ATAGAAGAAA AACGCAAATT ACTGTGTAGT GTGATTCCTC AAGCACCTCA GTACTATGAC 
GAATATGTAA CATTTAACGG ATCATATCTC CTGGATGGAA ACCCCTTAAG CAAGATTTGT 
ATTCCCACAA TTACACCACC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TAAGGATGAA ACTACGTTTG CAACACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAACG AACAAGAAGT TTTGCGAGTT CATTACAGAG CTGCAAGAAC ATTGGCAAAT 
CAAACACTGC CATTTAGTGC TTGTACTGTT TTGCTGGATG CCGAAGTATA CAATGTACCA 
TTGGACTCTC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAA 
TTCATGTCTT GGTTACAAGA TGTGGATGAT AAATTTGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AACATGAAGC TGCGGCTTTA AATGCTGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATTTACGA AATCCAGGAG 
TTTTATGTTC CCCTTGTTGA TGTTAACGAC GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258153.1
CDS	241..6354
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNNSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_005258096.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005258096.3

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAATTC CACACCAAAT CATCCATCAC AAACAACGCC TGCCCAAAAG 
AAAACTCCCA GTTCTTCATC TCGACAGAAA GATAAAGTTA ATAAAAGAAA TGAACGTGGT 
GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA AACAAGTTAA AGAATTAATA 
AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT GGACACCACT GCATGAAGCT 
TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG CAGCTGGAGC AGATGTTAAC 
ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG CTAGTAGTGG GCACAGAGAT 
ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC AAGCTAATAA ACATGGGGAG 
CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC TACTAAAAAG AGAGGTGCCT 
TTATCTGATG ATGATGAAAG TTACACAGAT TCCGAAGAGG CTCAATCTGT AAATCCTTCT 
AGTGTTGATG AAAATATTGA CTCTGAAACA GAGAAAGACT CTCTCATCTG TGAAAGTAAA 
CAGATACTTC CCAGTAAAAC ACCTCTTCCA TCTGCCCTTG ATGAGTATGA GTTCAAAGAT 
GATGATGATG AAGAAATTAA TAAGATGATT GATGATAGGC ATATTCTTAG GAAAGAACAA 
CGAAAAGAAA ATGAACCTGA AGCAGAAAAA ACTCATTTAT TTGCAAAACA GGAGAAAGCC 
TTCTATCCTA AATCATTTAA AAGTAAAAAA CAAAAGCCAT CTAGGGTCTT ATATTCAAGT 
ACTGAAAGTT CTGATGAAGA AGCTCTTCAG AATAAAAAGA TTTCTACTTC ATGTTCCGTC 
ATCCCTGAAA CATCAAATTC TGATATGCAA ACCAAAAAGG AATATGTAGT TTCAGGTGAA 
CACAAACAGA AAGGCAAAGT TAAAAGAAAA TTGAAAAATC AGAATAAAAA TAAAGAGAAC 
CAAGAGCTAA AGCAAGAAAA GGAAGGAAAA GAAAATACAA GAATAACAAA CTTGACAGTA 
AATACTGGAC TAGATTGTTC AGAAAAGACC AGAGAGGAGG GGAACTTTAG GAAATCTTTT 
AGCCCAAAAG ATGATACTTC ATTACATTTA TTTCATATTT CCACTGGTAA ATCTCCCAAA 
CATTCTTGTG GATTAAGTGA AAAACAGTCA ACACCACTAA AACAAGAACA TACTAAAACA 
TGTTTATCAC CAGGAAGTTC TGAAATGTCA TTACAGCCTG ATCTTGTTCG GTATGATAAT 
ACAGAATCTG AATTCTTGCC AGAAAGTTCA AGTGTAAAAT CTTGTAAGCA TAAGGAAAAA 
AGCAAACATC AGAAAGATTT CCACTTAGAA TTTGGTGAAA AATCAAATGC CAAAATAAAG 
GATGAAGATC ATAGTCCAAC ATTTGAAAAT TCAGATTGCA CACTGAAAAA AATGGATAAA 
GAAGGTAAAA CATTAAAAAA ACATAAATTG AAGCATAAAG AGAGGGAAAA AGAAAAGCAT 
AAAAAAGAAA TTGAAGGTGA AAAGGAAAAA TACAAAACTA AGGATAGTGC CAAAGAACTG 
CAGAGGAGTG TGGAATTTGA TAGAGAATTT TGGAAAGAGA ATTTTTTTAA AAGTGATGAA 
ACTGAAGATC TCTTTTTAAA TATGGAACAT GAATCCTTAA CATTAGAAAA AAAATCAAAA 
TTGGAAAAAA ACATCAAAGA TGATAAATCA ACCAAGGAAA AGCATGTGTC AAAAGAGAGG 
AACTTTAAAG AGGAACGAGA CAAGATTAAA AAGGAAAGCG AGAAATCTTT TAGGGAGGAA 
AAAATAAAAG ATCTAAAAGA AGAGAGAGAA AACATACCCA CAGATAAAGA CTCAGAATTT 
ACTTCTTTGG GTATGAGTGC CATTGAGGAA TCTATAGGGC TTCATTTAGT GGAAAAGGAA 
ATAGACATTG AAAAACAAGA AAAGCATATA AAGGAAAGTA AAGAAAAACC TGAGAAGCGA 
TCTCAAATTA AAGAAAAGGA CATTGAGAAG ATGGAAAGAA AAACCTTTGA AAAAGAAAAG 
AAGATAAAAC ATGAGCATAA GTCAGAAAAA GACAAATTAG ATCTTAGTGA ATGTGTTGAT 
AAAATAAAAG AAAAGGACAA GCTATATTCG CATCACACAG AAAAATGCCA TAAAGAAGGT 
GAGAAGAGCA AAAATACTGC TGCTATTAAA AAAACTGACG ACAGAGAGAA AAGTAGAGAA 
AAGATGGATA GGAAACATGA CAAAGAAAAG CCTGAAAAAG AGAGGCATCT AGCAGAAAGC 
AAAGAAAAGC ACTTGATGGA GAAAAAAAAT AAACAATCAG ATAATAGTGA ATACAGTAAA 
TCAGAAAAAG GCAAAAATAA AGAAAAAGAC AGGGAGCTAG ATAAAAAGGA AAAATCTAGA 
GATAAAGAAA GTATAAATAT AACTAACTCC AAACACATAC AGGAAGAAAA AAAATCAAGT 
ATAGTAGACG GTAATAAAGC ACAACATGAA AAACCCTTAT CCCTTAAAGA AAAAACAAAA 
GATGAACCTT TGAAAACTCC AGATGGAAAA GAAAAAGATA AAAAAGATAA AGATATAGAT 
AGATACAAAG AACGAGACAA ACATAAAGAT AAAATTCAAA TAAATAGCTT ACTCAAACTA 
AAATCTGAAG CAGATAAGCC TAAACCTAAG TCATCACCAG CATCAAAAGA TACCCGACCT 
AAAGAAAAGA GGTTAGTGAA TGATGATTTA ATGCAGACAA GTTTTGAACG AATGCTAAGC 
CTTAAAGACC TAGAAATAGA ACAGTGGCAC AAAAAACATA AGGAAAAAAT TAAGCAAAAA 
GAAAAGGAAC GGTTGAGAAA CCGAAACTGT TTAGAACTTA AAATAAAAGA TAAAGAAAAA 
ACAAAGCATA CACCAACTGA ATCCAAAAAT AAAGAACTTA CTAGGTCAAA GAGTTCAGAA 
GTGACTGATG CATATACCAA GGAGAAACAA CCTAAAGATG CTGTAAGTAA CAGATCACAA 
TCTGTTGACA CCAAAAATGT AATGACTTTA GGGAAGTCAT CTTTTGTTTC AGATAATAGC 
TTAAACAGGT CTCCTAGATC AGAAAATGAA AAGCCGGGTC TCAGCTCCAG ATCTGTATCC 
ATGATTTCTG TTGCTAGTTC AGAAGATTCC TGCCATACTA CAGTGACAAC CCCAAGGCCT 
CCAGTTGAGT ATGACTCTGA CTTTATGTTA GAGAGTTCAG AATCCCAAAT GTCCTTTTCC 
CAGTCACCTT TTTTGTCAAT TGCCAAATCT CCTGCTCTTC ATGAAAGGGA ATTGGACAGC 
CTGGCTGACT TGCCGGAGCG GATTAAACCA CCATATGCAA ACAGACTTTC AACATCCCAT 
CTTAGGTCAT CTTCTGTAGA AGATGTTAAA CTAATTATAA GCGAGGGGAG ACCTACCATA 
GAAGTTCGAA GATGTAGCAT GCCTTCTGTC ATTTGTGAAC ATACCAAACA ATTCCAAACA 
ATATCAGAAG AGAGCAATCA AGGTAGCTTA TTAACTGTGC CAGGAGATAC TAGTCCTTCT 
CCCAAACCTG AGGTATTCTC AAATGTGCCT GAAAGAGACC TTTCAAATGT ATCTAACATA 
CATTCCAGTT TTGCAACTTC TCCAACTGGA GCTTCAAACA GCAAGTATGT TTCAGCTGAT 
AGAAATCTCA TCAAGAATAC TGCCCCAGTG AACACTGTAA TGGACAGTCC AGTGCATTTA 
GAGCCATCTA GTCAGGTTGG TGTGATCCAG AATAAATCAT GGGAGATGCC TGTTGATAGA 
CTAGAGACAT TAAGCACCAG AGACTTTATC TGCCCAAATT CTAACATACC TGATCAAGAA 
TCCTCTCTTC AGAGTTTTTG TAATTCTGAA AATAAGGTAT TGAAAGAAAA TGCTGATTTT 
TTATCCCTGC GCCAGACTGA ACTGCCAGGA AACTCTTGTG CTCAGGATCC GGCATCCTTT 
ATGCCTCCAC AGCAGCCTTG CTCTTTCCCC AGCCAATCAC TTTCAGATGC TGAATCGATT 
TCTAAACATA TGTCTTTGTC ATATGTTGCT AATCAAGAGC CAGGTATTTT ACAACAAAAA 
AATGCAGTTC AGATTATTAG TTCTGCTTTA GATACTGATA ATGAATCTAC AAAAGATACA 
GAAAATACTT TTGTCCTAGG AGATGTTCAA AAAACAGATG CCTTTGTCCC AGTGTACTCT 
GACAGCACTA TTCAAGAAGC ATCACCAAAC TTTGAGAAAG CTTATACTTT ACCTGTGTTA 
CCATCAGAAA AGGACTTTAA TGGAAGTGAT GCCTCTACCC AGCTAAATAC ACATTATGCA 
TTTAGCAAAC TAACTTACAA GTCTTCCAGT GGCCATGAAG TTGAGAATAG CACAACTGAT 
ACTCAGGTCA TTTCACATGA AAAAGAAAAC AAACTGGAGA GTTTGGTTTT AACTCATTTG 
AGTAGGTGTG ATTCTGATTT ATGTGAAATG AATGCAGGGA TGCCAAAAGG AAACCTAAAT 
GAACAAGATC CAAAACATTG TCCTGAAAGT GAAAAGTGTT TGCTTTCCAT AGAAGATGAG 
GAATCTCAAC AAAGCATTTT ATCAAGTCTG GAAAACCATT CACAGCAGTC AACTCAACCA 
GAAATGCATA AATATGGTCA GTTAGTTAAA GTAGAATTAG AAGAAAATGC CGAAGATGAT 
AAAACTGAAA ACCAAATCCC TCAAAGAATG ACTAGAAACA AAGCAAATAC AATGGCAAAT 
CAAAGCAAAC AGATTCTTGC TAGCTGTACA CTATTATCAG AAAAAGACAG TGAATCCTCA 
TCTCCTAGAG GAAGAATAAG ATTAACTGAA GATGACGATC CTCAAATTCA CCATCCACGG 
AAAAGGAAAG TGTCACGTGT ACCTCAGCCT GTGCAAGTGA GTCCCTCTTT ACTACAAGCA 
AAAGAGAAAA CTCAGCAATC TCTGGCAGCC ATTGTAGATT CTCTAAAACT AGATGAGATT 
CAGCCATACA GTTCAGAGAG AGCAAATCCA TATTTTGAAT ACTTGCACAT AAGGAAAAAA 
ATAGAAGAAA AACGCAAATT ACTGTGTAGT GTGATTCCTC AAGCACCTCA GTACTATGAC 
GAATATGTAA CATTTAACGG ATCATATCTC CTGGATGGAA ACCCCTTAAG CAAGATTTGT 
ATTCCCACAA TTACACCACC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TAAGGATGAA ACTACGTTTG CAACACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAACG AACAAGAAGT TTTGCGAGTT CATTACAGAG CTGCAAGAAC ATTGGCAAAT 
CAAACACTGC CATTTAGTGC TTGTACTGTT TTGCTGGATG CCGAAGTATA CAATGTACCA 
TTGGACTCTC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAA 
TTCATGTCTT GGTTACAAGA TGTGGATGAT AAATTTGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AACATGAAGC TGCGGCTTTA AATGCTGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATTTACGA AATCCAGGAG 
TTTTATGTTC CCCTTGTTGA TGTTAACGAC GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_005258096.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258153.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X7, mRNA.

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CloneID	OHu50997
Clone ID Related Accession (Same CDS sequence)	XM_017025666.1 , XM_017025664.2 , XM_017025665.2 , XM_017025666.2 , XM_017025664.1 , XM_005258096.3 , XM_005258096.2 , XM_017025665.1
Accession Version	XM_005258096.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005258096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAATTC CACACCAAAT CATCCATCAC AAACAACGCC TGCCCAAAAG 
AAAACTCCCA GTTCTTCATC TCGACAGAAA GATAAAGTTA ATAAAAGAAA TGAACGTGGT 
GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA AACAAGTTAA AGAATTAATA 
AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT GGACACCACT GCATGAAGCT 
TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG CAGCTGGAGC AGATGTTAAC 
ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG CTAGTAGTGG GCACAGAGAT 
ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC AAGCTAATAA ACATGGGGAG 
CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC TACTAAAAAG AGAGGTGCCT 
TTATCTGATG ATGATGAAAG TTACACAGAT TCCGAAGAGG CTCAATCTGT AAATCCTTCT 
AGTGTTGATG AAAATATTGA CTCTGAAACA GAGAAAGACT CTCTCATCTG TGAAAGTAAA 
CAGATACTTC CCAGTAAAAC ACCTCTTCCA TCTGCCCTTG ATGAGTATGA GTTCAAAGAT 
GATGATGATG AAGAAATTAA TAAGATGATT GATGATAGGC ATATTCTTAG GAAAGAACAA 
CGAAAAGAAA ATGAACCTGA AGCAGAAAAA ACTCATTTAT TTGCAAAACA GGAGAAAGCC 
TTCTATCCTA AATCATTTAA AAGTAAAAAA CAAAAGCCAT CTAGGGTCTT ATATTCAAGT 
ACTGAAAGTT CTGATGAAGA AGCTCTTCAG AATAAAAAGA TTTCTACTTC ATGTTCCGTC 
ATCCCTGAAA CATCAAATTC TGATATGCAA ACCAAAAAGG AATATGTAGT TTCAGGTGAA 
CACAAACAGA AAGGCAAAGT TAAAAGAAAA TTGAAAAATC AGAATAAAAA TAAAGAGAAC 
CAAGAGCTAA AGCAAGAAAA GGAAGGAAAA GAAAATACAA GAATAACAAA CTTGACAGTA 
AATACTGGAC TAGATTGTTC AGAAAAGACC AGAGAGGAGG GGAACTTTAG GAAATCTTTT 
AGCCCAAAAG ATGATACTTC ATTACATTTA TTTCATATTT CCACTGGTAA ATCTCCCAAA 
CATTCTTGTG GATTAAGTGA AAAACAGTCA ACACCACTAA AACAAGAACA TACTAAAACA 
TGTTTATCAC CAGGAAGTTC TGAAATGTCA TTACAGCCTG ATCTTGTTCG GTATGATAAT 
ACAGAATCTG AATTCTTGCC AGAAAGTTCA AGTGTAAAAT CTTGTAAGCA TAAGGAAAAA 
AGCAAACATC AGAAAGATTT CCACTTAGAA TTTGGTGAAA AATCAAATGC CAAAATAAAG 
GATGAAGATC ATAGTCCAAC ATTTGAAAAT TCAGATTGCA CACTGAAAAA AATGGATAAA 
GAAGGTAAAA CATTAAAAAA ACATAAATTG AAGCATAAAG AGAGGGAAAA AGAAAAGCAT 
AAAAAAGAAA TTGAAGGTGA AAAGGAAAAA TACAAAACTA AGGATAGTGC CAAAGAACTG 
CAGAGGAGTG TGGAATTTGA TAGAGAATTT TGGAAAGAGA ATTTTTTTAA AAGTGATGAA 
ACTGAAGATC TCTTTTTAAA TATGGAACAT GAATCCTTAA CATTAGAAAA AAAATCAAAA 
TTGGAAAAAA ACATCAAAGA TGATAAATCA ACCAAGGAAA AGCATGTGTC AAAAGAGAGG 
AACTTTAAAG AGGAACGAGA CAAGATTAAA AAGGAAAGCG AGAAATCTTT TAGGGAGGAA 
AAAATAAAAG ATCTAAAAGA AGAGAGAGAA AACATACCCA CAGATAAAGA CTCAGAATTT 
ACTTCTTTGG GTATGAGTGC CATTGAGGAA TCTATAGGGC TTCATTTAGT GGAAAAGGAA 
ATAGACATTG AAAAACAAGA AAAGCATATA AAGGAAAGTA AAGAAAAACC TGAGAAGCGA 
TCTCAAATTA AAGAAAAGGA CATTGAGAAG ATGGAAAGAA AAACCTTTGA AAAAGAAAAG 
AAGATAAAAC ATGAGCATAA GTCAGAAAAA GACAAATTAG ATCTTAGTGA ATGTGTTGAT 
AAAATAAAAG AAAAGGACAA GCTATATTCG CATCACACAG AAAAATGCCA TAAAGAAGGT 
GAGAAGAGCA AAAATACTGC TGCTATTAAA AAAACTGACG ACAGAGAGAA AAGTAGAGAA 
AAGATGGATA GGAAACATGA CAAAGAAAAG CCTGAAAAAG AGAGGCATCT AGCAGAAAGC 
AAAGAAAAGC ACTTGATGGA GAAAAAAAAT AAACAATCAG ATAATAGTGA ATACAGTAAA 
TCAGAAAAAG GCAAAAATAA AGAAAAAGAC AGGGAGCTAG ATAAAAAGGA AAAATCTAGA 
GATAAAGAAA GTATAAATAT AACTAACTCC AAACACATAC AGGAAGAAAA AAAATCAAGT 
ATAGTAGACG GTAATAAAGC ACAACATGAA AAACCCTTAT CCCTTAAAGA AAAAACAAAA 
GATGAACCTT TGAAAACTCC AGATGGAAAA GAAAAAGATA AAAAAGATAA AGATATAGAT 
AGATACAAAG AACGAGACAA ACATAAAGAT AAAATTCAAA TAAATAGCTT ACTCAAACTA 
AAATCTGAAG CAGATAAGCC TAAACCTAAG TCATCACCAG CATCAAAAGA TACCCGACCT 
AAAGAAAAGA GGTTAGTGAA TGATGATTTA ATGCAGACAA GTTTTGAACG AATGCTAAGC 
CTTAAAGACC TAGAAATAGA ACAGTGGCAC AAAAAACATA AGGAAAAAAT TAAGCAAAAA 
GAAAAGGAAC GGTTGAGAAA CCGAAACTGT TTAGAACTTA AAATAAAAGA TAAAGAAAAA 
ACAAAGCATA CACCAACTGA ATCCAAAAAT AAAGAACTTA CTAGGTCAAA GAGTTCAGAA 
GTGACTGATG CATATACCAA GGAGAAACAA CCTAAAGATG CTGTAAGTAA CAGATCACAA 
TCTGTTGACA CCAAAAATGT AATGACTTTA GGGAAGTCAT CTTTTGTTTC AGATAATAGC 
TTAAACAGGT CTCCTAGATC AGAAAATGAA AAGCCGGGTC TCAGCTCCAG ATCTGTATCC 
ATGATTTCTG TTGCTAGTTC AGAAGATTCC TGCCATACTA CAGTGACAAC CCCAAGGCCT 
CCAGTTGAGT ATGACTCTGA CTTTATGTTA GAGAGTTCAG AATCCCAAAT GTCCTTTTCC 
CAGTCACCTT TTTTGTCAAT TGCCAAATCT CCTGCTCTTC ATGAAAGGGA ATTGGACAGC 
CTGGCTGACT TGCCGGAGCG GATTAAACCA CCATATGCAA ACAGACTTTC AACATCCCAT 
CTTAGGTCAT CTTCTGTAGA AGATGTTAAA CTAATTATAA GCGAGGGGAG ACCTACCATA 
GAAGTTCGAA GATGTAGCAT GCCTTCTGTC ATTTGTGAAC ATACCAAACA ATTCCAAACA 
ATATCAGAAG AGAGCAATCA AGGTAGCTTA TTAACTGTGC CAGGAGATAC TAGTCCTTCT 
CCCAAACCTG AGGTATTCTC AAATGTGCCT GAAAGAGACC TTTCAAATGT ATCTAACATA 
CATTCCAGTT TTGCAACTTC TCCAACTGGA GCTTCAAACA GCAAGTATGT TTCAGCTGAT 
AGAAATCTCA TCAAGAATAC TGCCCCAGTG AACACTGTAA TGGACAGTCC AGTGCATTTA 
GAGCCATCTA GTCAGGTTGG TGTGATCCAG AATAAATCAT GGGAGATGCC TGTTGATAGA 
CTAGAGACAT TAAGCACCAG AGACTTTATC TGCCCAAATT CTAACATACC TGATCAAGAA 
TCCTCTCTTC AGAGTTTTTG TAATTCTGAA AATAAGGTAT TGAAAGAAAA TGCTGATTTT 
TTATCCCTGC GCCAGACTGA ACTGCCAGGA AACTCTTGTG CTCAGGATCC GGCATCCTTT 
ATGCCTCCAC AGCAGCCTTG CTCTTTCCCC AGCCAATCAC TTTCAGATGC TGAATCGATT 
TCTAAACATA TGTCTTTGTC ATATGTTGCT AATCAAGAGC CAGGTATTTT ACAACAAAAA 
AATGCAGTTC AGATTATTAG TTCTGCTTTA GATACTGATA ATGAATCTAC AAAAGATACA 
GAAAATACTT TTGTCCTAGG AGATGTTCAA AAAACAGATG CCTTTGTCCC AGTGTACTCT 
GACAGCACTA TTCAAGAAGC ATCACCAAAC TTTGAGAAAG CTTATACTTT ACCTGTGTTA 
CCATCAGAAA AGGACTTTAA TGGAAGTGAT GCCTCTACCC AGCTAAATAC ACATTATGCA 
TTTAGCAAAC TAACTTACAA GTCTTCCAGT GGCCATGAAG TTGAGAATAG CACAACTGAT 
ACTCAGGTCA TTTCACATGA AAAAGAAAAC AAACTGGAGA GTTTGGTTTT AACTCATTTG 
AGTAGGTGTG ATTCTGATTT ATGTGAAATG AATGCAGGGA TGCCAAAAGG AAACCTAAAT 
GAACAAGATC CAAAACATTG TCCTGAAAGT GAAAAGTGTT TGCTTTCCAT AGAAGATGAG 
GAATCTCAAC AAAGCATTTT ATCAAGTCTG GAAAACCATT CACAGCAGTC AACTCAACCA 
GAAATGCATA AATATGGTCA GTTAGTTAAA GTAGAATTAG AAGAAAATGC CGAAGATGAT 
AAAACTGAAA ACCAAATCCC TCAAAGAATG ACTAGAAACA AAGCAAATAC AATGGCAAAT 
CAAAGCAAAC AGATTCTTGC TAGCTGTACA CTATTATCAG AAAAAGACAG TGAATCCTCA 
TCTCCTAGAG GAAGAATAAG ATTAACTGAA GATGACGATC CTCAAATTCA CCATCCACGG 
AAAAGGAAAG TGTCACGTGT ACCTCAGCCT GTGCAAGTGA GTCCCTCTTT ACTACAAGCA 
AAAGAGAAAA CTCAGCAATC TCTGGCAGCC ATTGTAGATT CTCTAAAACT AGATGAGATT 
CAGCCATACA GTTCAGAGAG AGCAAATCCA TATTTTGAAT ACTTGCACAT AAGGAAAAAA 
ATAGAAGAAA AACGCAAATT ACTGTGTAGT GTGATTCCTC AAGCACCTCA GTACTATGAC 
GAATATGTAA CATTTAACGG ATCATATCTC CTGGATGGAA ACCCCTTAAG CAAGATTTGT 
ATTCCCACAA TTACACCACC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TAAGGATGAA ACTACGTTTG CAACACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAACG AACAAGAAGT TTTGCGAGTT CATTACAGAG CTGCAAGAAC ATTGGCAAAT 
CAAACACTGC CATTTAGTGC TTGTACTGTT TTGCTGGATG CCGAAGTATA CAATGTACCA 
TTGGACTCTC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAA 
TTCATGTCTT GGTTACAAGA TGTGGATGAT AAATTTGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AACATGAAGC TGCGGCTTTA AATGCTGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATTTACGA AATCCAGGAG 
TTTTATGTTC CCCTTGTTGA TGTTAACGAC GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258153.1
CDS	241..6354
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNNSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_005258096.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005258096.2

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAATTC CACACCAAAT CATCCATCAC AAACAACGCC TGCCCAAAAG 
AAAACTCCCA GTTCTTCATC TCGACAGAAA GATAAAGTTA ATAAAAGAAA TGAACGTGGT 
GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA AACAAGTTAA AGAATTAATA 
AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT GGACACCACT GCATGAAGCT 
TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG CAGCTGGAGC AGATGTTAAC 
ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG CTAGTAGTGG GCACAGAGAT 
ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC AAGCTAATAA ACATGGGGAG 
CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC TACTAAAAAG AGAGGTGCCT 
TTATCTGATG ATGATGAAAG TTACACAGAT TCCGAAGAGG CTCAATCTGT AAATCCTTCT 
AGTGTTGATG AAAATATTGA CTCTGAAACA GAGAAAGACT CTCTCATCTG TGAAAGTAAA 
CAGATACTTC CCAGTAAAAC ACCTCTTCCA TCTGCCCTTG ATGAGTATGA GTTCAAAGAT 
GATGATGATG AAGAAATTAA TAAGATGATT GATGATAGGC ATATTCTTAG GAAAGAACAA 
CGAAAAGAAA ATGAACCTGA AGCAGAAAAA ACTCATTTAT TTGCAAAACA GGAGAAAGCC 
TTCTATCCTA AATCATTTAA AAGTAAAAAA CAAAAGCCAT CTAGGGTCTT ATATTCAAGT 
ACTGAAAGTT CTGATGAAGA AGCTCTTCAG AATAAAAAGA TTTCTACTTC ATGTTCCGTC 
ATCCCTGAAA CATCAAATTC TGATATGCAA ACCAAAAAGG AATATGTAGT TTCAGGTGAA 
CACAAACAGA AAGGCAAAGT TAAAAGAAAA TTGAAAAATC AGAATAAAAA TAAAGAGAAC 
CAAGAGCTAA AGCAAGAAAA GGAAGGAAAA GAAAATACAA GAATAACAAA CTTGACAGTA 
AATACTGGAC TAGATTGTTC AGAAAAGACC AGAGAGGAGG GGAACTTTAG GAAATCTTTT 
AGCCCAAAAG ATGATACTTC ATTACATTTA TTTCATATTT CCACTGGTAA ATCTCCCAAA 
CATTCTTGTG GATTAAGTGA AAAACAGTCA ACACCACTAA AACAAGAACA TACTAAAACA 
TGTTTATCAC CAGGAAGTTC TGAAATGTCA TTACAGCCTG ATCTTGTTCG GTATGATAAT 
ACAGAATCTG AATTCTTGCC AGAAAGTTCA AGTGTAAAAT CTTGTAAGCA TAAGGAAAAA 
AGCAAACATC AGAAAGATTT CCACTTAGAA TTTGGTGAAA AATCAAATGC CAAAATAAAG 
GATGAAGATC ATAGTCCAAC ATTTGAAAAT TCAGATTGCA CACTGAAAAA AATGGATAAA 
GAAGGTAAAA CATTAAAAAA ACATAAATTG AAGCATAAAG AGAGGGAAAA AGAAAAGCAT 
AAAAAAGAAA TTGAAGGTGA AAAGGAAAAA TACAAAACTA AGGATAGTGC CAAAGAACTG 
CAGAGGAGTG TGGAATTTGA TAGAGAATTT TGGAAAGAGA ATTTTTTTAA AAGTGATGAA 
ACTGAAGATC TCTTTTTAAA TATGGAACAT GAATCCTTAA CATTAGAAAA AAAATCAAAA 
TTGGAAAAAA ACATCAAAGA TGATAAATCA ACCAAGGAAA AGCATGTGTC AAAAGAGAGG 
AACTTTAAAG AGGAACGAGA CAAGATTAAA AAGGAAAGCG AGAAATCTTT TAGGGAGGAA 
AAAATAAAAG ATCTAAAAGA AGAGAGAGAA AACATACCCA CAGATAAAGA CTCAGAATTT 
ACTTCTTTGG GTATGAGTGC CATTGAGGAA TCTATAGGGC TTCATTTAGT GGAAAAGGAA 
ATAGACATTG AAAAACAAGA AAAGCATATA AAGGAAAGTA AAGAAAAACC TGAGAAGCGA 
TCTCAAATTA AAGAAAAGGA CATTGAGAAG ATGGAAAGAA AAACCTTTGA AAAAGAAAAG 
AAGATAAAAC ATGAGCATAA GTCAGAAAAA GACAAATTAG ATCTTAGTGA ATGTGTTGAT 
AAAATAAAAG AAAAGGACAA GCTATATTCG CATCACACAG AAAAATGCCA TAAAGAAGGT 
GAGAAGAGCA AAAATACTGC TGCTATTAAA AAAACTGACG ACAGAGAGAA AAGTAGAGAA 
AAGATGGATA GGAAACATGA CAAAGAAAAG CCTGAAAAAG AGAGGCATCT AGCAGAAAGC 
AAAGAAAAGC ACTTGATGGA GAAAAAAAAT AAACAATCAG ATAATAGTGA ATACAGTAAA 
TCAGAAAAAG GCAAAAATAA AGAAAAAGAC AGGGAGCTAG ATAAAAAGGA AAAATCTAGA 
GATAAAGAAA GTATAAATAT AACTAACTCC AAACACATAC AGGAAGAAAA AAAATCAAGT 
ATAGTAGACG GTAATAAAGC ACAACATGAA AAACCCTTAT CCCTTAAAGA AAAAACAAAA 
GATGAACCTT TGAAAACTCC AGATGGAAAA GAAAAAGATA AAAAAGATAA AGATATAGAT 
AGATACAAAG AACGAGACAA ACATAAAGAT AAAATTCAAA TAAATAGCTT ACTCAAACTA 
AAATCTGAAG CAGATAAGCC TAAACCTAAG TCATCACCAG CATCAAAAGA TACCCGACCT 
AAAGAAAAGA GGTTAGTGAA TGATGATTTA ATGCAGACAA GTTTTGAACG AATGCTAAGC 
CTTAAAGACC TAGAAATAGA ACAGTGGCAC AAAAAACATA AGGAAAAAAT TAAGCAAAAA 
GAAAAGGAAC GGTTGAGAAA CCGAAACTGT TTAGAACTTA AAATAAAAGA TAAAGAAAAA 
ACAAAGCATA CACCAACTGA ATCCAAAAAT AAAGAACTTA CTAGGTCAAA GAGTTCAGAA 
GTGACTGATG CATATACCAA GGAGAAACAA CCTAAAGATG CTGTAAGTAA CAGATCACAA 
TCTGTTGACA CCAAAAATGT AATGACTTTA GGGAAGTCAT CTTTTGTTTC AGATAATAGC 
TTAAACAGGT CTCCTAGATC AGAAAATGAA AAGCCGGGTC TCAGCTCCAG ATCTGTATCC 
ATGATTTCTG TTGCTAGTTC AGAAGATTCC TGCCATACTA CAGTGACAAC CCCAAGGCCT 
CCAGTTGAGT ATGACTCTGA CTTTATGTTA GAGAGTTCAG AATCCCAAAT GTCCTTTTCC 
CAGTCACCTT TTTTGTCAAT TGCCAAATCT CCTGCTCTTC ATGAAAGGGA ATTGGACAGC 
CTGGCTGACT TGCCGGAGCG GATTAAACCA CCATATGCAA ACAGACTTTC AACATCCCAT 
CTTAGGTCAT CTTCTGTAGA AGATGTTAAA CTAATTATAA GCGAGGGGAG ACCTACCATA 
GAAGTTCGAA GATGTAGCAT GCCTTCTGTC ATTTGTGAAC ATACCAAACA ATTCCAAACA 
ATATCAGAAG AGAGCAATCA AGGTAGCTTA TTAACTGTGC CAGGAGATAC TAGTCCTTCT 
CCCAAACCTG AGGTATTCTC AAATGTGCCT GAAAGAGACC TTTCAAATGT ATCTAACATA 
CATTCCAGTT TTGCAACTTC TCCAACTGGA GCTTCAAACA GCAAGTATGT TTCAGCTGAT 
AGAAATCTCA TCAAGAATAC TGCCCCAGTG AACACTGTAA TGGACAGTCC AGTGCATTTA 
GAGCCATCTA GTCAGGTTGG TGTGATCCAG AATAAATCAT GGGAGATGCC TGTTGATAGA 
CTAGAGACAT TAAGCACCAG AGACTTTATC TGCCCAAATT CTAACATACC TGATCAAGAA 
TCCTCTCTTC AGAGTTTTTG TAATTCTGAA AATAAGGTAT TGAAAGAAAA TGCTGATTTT 
TTATCCCTGC GCCAGACTGA ACTGCCAGGA AACTCTTGTG CTCAGGATCC GGCATCCTTT 
ATGCCTCCAC AGCAGCCTTG CTCTTTCCCC AGCCAATCAC TTTCAGATGC TGAATCGATT 
TCTAAACATA TGTCTTTGTC ATATGTTGCT AATCAAGAGC CAGGTATTTT ACAACAAAAA 
AATGCAGTTC AGATTATTAG TTCTGCTTTA GATACTGATA ATGAATCTAC AAAAGATACA 
GAAAATACTT TTGTCCTAGG AGATGTTCAA AAAACAGATG CCTTTGTCCC AGTGTACTCT 
GACAGCACTA TTCAAGAAGC ATCACCAAAC TTTGAGAAAG CTTATACTTT ACCTGTGTTA 
CCATCAGAAA AGGACTTTAA TGGAAGTGAT GCCTCTACCC AGCTAAATAC ACATTATGCA 
TTTAGCAAAC TAACTTACAA GTCTTCCAGT GGCCATGAAG TTGAGAATAG CACAACTGAT 
ACTCAGGTCA TTTCACATGA AAAAGAAAAC AAACTGGAGA GTTTGGTTTT AACTCATTTG 
AGTAGGTGTG ATTCTGATTT ATGTGAAATG AATGCAGGGA TGCCAAAAGG AAACCTAAAT 
GAACAAGATC CAAAACATTG TCCTGAAAGT GAAAAGTGTT TGCTTTCCAT AGAAGATGAG 
GAATCTCAAC AAAGCATTTT ATCAAGTCTG GAAAACCATT CACAGCAGTC AACTCAACCA 
GAAATGCATA AATATGGTCA GTTAGTTAAA GTAGAATTAG AAGAAAATGC CGAAGATGAT 
AAAACTGAAA ACCAAATCCC TCAAAGAATG ACTAGAAACA AAGCAAATAC AATGGCAAAT 
CAAAGCAAAC AGATTCTTGC TAGCTGTACA CTATTATCAG AAAAAGACAG TGAATCCTCA 
TCTCCTAGAG GAAGAATAAG ATTAACTGAA GATGACGATC CTCAAATTCA CCATCCACGG 
AAAAGGAAAG TGTCACGTGT ACCTCAGCCT GTGCAAGTGA GTCCCTCTTT ACTACAAGCA 
AAAGAGAAAA CTCAGCAATC TCTGGCAGCC ATTGTAGATT CTCTAAAACT AGATGAGATT 
CAGCCATACA GTTCAGAGAG AGCAAATCCA TATTTTGAAT ACTTGCACAT AAGGAAAAAA 
ATAGAAGAAA AACGCAAATT ACTGTGTAGT GTGATTCCTC AAGCACCTCA GTACTATGAC 
GAATATGTAA CATTTAACGG ATCATATCTC CTGGATGGAA ACCCCTTAAG CAAGATTTGT 
ATTCCCACAA TTACACCACC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TAAGGATGAA ACTACGTTTG CAACACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAACG AACAAGAAGT TTTGCGAGTT CATTACAGAG CTGCAAGAAC ATTGGCAAAT 
CAAACACTGC CATTTAGTGC TTGTACTGTT TTGCTGGATG CCGAAGTATA CAATGTACCA 
TTGGACTCTC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAA 
TTCATGTCTT GGTTACAAGA TGTGGATGAT AAATTTGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AACATGAAGC TGCGGCTTTA AATGCTGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATTTACGA AATCCAGGAG 
TTTTATGTTC CCCTTGTTGA TGTTAACGAC GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025665.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881154.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X9, mRNA.

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CloneID	OHu50997
Clone ID Related Accession (Same CDS sequence)	XM_017025666.1 , XM_017025664.2 , XM_017025665.2 , XM_017025666.2 , XM_017025664.1 , XM_005258096.3 , XM_005258096.2 , XM_017025665.1
Accession Version	XM_017025665.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAATTC CACACCAAAT CATCCATCAC AAACAACGCC TGCCCAAAAG 
AAAACTCCCA GTTCTTCATC TCGACAGAAA GATAAAGTTA ATAAAAGAAA TGAACGTGGT 
GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA AACAAGTTAA AGAATTAATA 
AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT GGACACCACT GCATGAAGCT 
TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG CAGCTGGAGC AGATGTTAAC 
ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG CTAGTAGTGG GCACAGAGAT 
ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC AAGCTAATAA ACATGGGGAG 
CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC TACTAAAAAG AGAGGTGCCT 
TTATCTGATG ATGATGAAAG TTACACAGAT TCCGAAGAGG CTCAATCTGT AAATCCTTCT 
AGTGTTGATG AAAATATTGA CTCTGAAACA GAGAAAGACT CTCTCATCTG TGAAAGTAAA 
CAGATACTTC CCAGTAAAAC ACCTCTTCCA TCTGCCCTTG ATGAGTATGA GTTCAAAGAT 
GATGATGATG AAGAAATTAA TAAGATGATT GATGATAGGC ATATTCTTAG GAAAGAACAA 
CGAAAAGAAA ATGAACCTGA AGCAGAAAAA ACTCATTTAT TTGCAAAACA GGAGAAAGCC 
TTCTATCCTA AATCATTTAA AAGTAAAAAA CAAAAGCCAT CTAGGGTCTT ATATTCAAGT 
ACTGAAAGTT CTGATGAAGA AGCTCTTCAG AATAAAAAGA TTTCTACTTC ATGTTCCGTC 
ATCCCTGAAA CATCAAATTC TGATATGCAA ACCAAAAAGG AATATGTAGT TTCAGGTGAA 
CACAAACAGA AAGGCAAAGT TAAAAGAAAA TTGAAAAATC AGAATAAAAA TAAAGAGAAC 
CAAGAGCTAA AGCAAGAAAA GGAAGGAAAA GAAAATACAA GAATAACAAA CTTGACAGTA 
AATACTGGAC TAGATTGTTC AGAAAAGACC AGAGAGGAGG GGAACTTTAG GAAATCTTTT 
AGCCCAAAAG ATGATACTTC ATTACATTTA TTTCATATTT CCACTGGTAA ATCTCCCAAA 
CATTCTTGTG GATTAAGTGA AAAACAGTCA ACACCACTAA AACAAGAACA TACTAAAACA 
TGTTTATCAC CAGGAAGTTC TGAAATGTCA TTACAGCCTG ATCTTGTTCG GTATGATAAT 
ACAGAATCTG AATTCTTGCC AGAAAGTTCA AGTGTAAAAT CTTGTAAGCA TAAGGAAAAA 
AGCAAACATC AGAAAGATTT CCACTTAGAA TTTGGTGAAA AATCAAATGC CAAAATAAAG 
GATGAAGATC ATAGTCCAAC ATTTGAAAAT TCAGATTGCA CACTGAAAAA AATGGATAAA 
GAAGGTAAAA CATTAAAAAA ACATAAATTG AAGCATAAAG AGAGGGAAAA AGAAAAGCAT 
AAAAAAGAAA TTGAAGGTGA AAAGGAAAAA TACAAAACTA AGGATAGTGC CAAAGAACTG 
CAGAGGAGTG TGGAATTTGA TAGAGAATTT TGGAAAGAGA ATTTTTTTAA AAGTGATGAA 
ACTGAAGATC TCTTTTTAAA TATGGAACAT GAATCCTTAA CATTAGAAAA AAAATCAAAA 
TTGGAAAAAA ACATCAAAGA TGATAAATCA ACCAAGGAAA AGCATGTGTC AAAAGAGAGG 
AACTTTAAAG AGGAACGAGA CAAGATTAAA AAGGAAAGCG AGAAATCTTT TAGGGAGGAA 
AAAATAAAAG ATCTAAAAGA AGAGAGAGAA AACATACCCA CAGATAAAGA CTCAGAATTT 
ACTTCTTTGG GTATGAGTGC CATTGAGGAA TCTATAGGGC TTCATTTAGT GGAAAAGGAA 
ATAGACATTG AAAAACAAGA AAAGCATATA AAGGAAAGTA AAGAAAAACC TGAGAAGCGA 
TCTCAAATTA AAGAAAAGGA CATTGAGAAG ATGGAAAGAA AAACCTTTGA AAAAGAAAAG 
AAGATAAAAC ATGAGCATAA GTCAGAAAAA GACAAATTAG ATCTTAGTGA ATGTGTTGAT 
AAAATAAAAG AAAAGGACAA GCTATATTCG CATCACACAG AAAAATGCCA TAAAGAAGGT 
GAGAAGAGCA AAAATACTGC TGCTATTAAA AAAACTGACG ACAGAGAGAA AAGTAGAGAA 
AAGATGGATA GGAAACATGA CAAAGAAAAG CCTGAAAAAG AGAGGCATCT AGCAGAAAGC 
AAAGAAAAGC ACTTGATGGA GAAAAAAAAT AAACAATCAG ATAATAGTGA ATACAGTAAA 
TCAGAAAAAG GCAAAAATAA AGAAAAAGAC AGGGAGCTAG ATAAAAAGGA AAAATCTAGA 
GATAAAGAAA GTATAAATAT AACTAACTCC AAACACATAC AGGAAGAAAA AAAATCAAGT 
ATAGTAGACG GTAATAAAGC ACAACATGAA AAACCCTTAT CCCTTAAAGA AAAAACAAAA 
GATGAACCTT TGAAAACTCC AGATGGAAAA GAAAAAGATA AAAAAGATAA AGATATAGAT 
AGATACAAAG AACGAGACAA ACATAAAGAT AAAATTCAAA TAAATAGCTT ACTCAAACTA 
AAATCTGAAG CAGATAAGCC TAAACCTAAG TCATCACCAG CATCAAAAGA TACCCGACCT 
AAAGAAAAGA GGTTAGTGAA TGATGATTTA ATGCAGACAA GTTTTGAACG AATGCTAAGC 
CTTAAAGACC TAGAAATAGA ACAGTGGCAC AAAAAACATA AGGAAAAAAT TAAGCAAAAA 
GAAAAGGAAC GGTTGAGAAA CCGAAACTGT TTAGAACTTA AAATAAAAGA TAAAGAAAAA 
ACAAAGCATA CACCAACTGA ATCCAAAAAT AAAGAACTTA CTAGGTCAAA GAGTTCAGAA 
GTGACTGATG CATATACCAA GGAGAAACAA CCTAAAGATG CTGTAAGTAA CAGATCACAA 
TCTGTTGACA CCAAAAATGT AATGACTTTA GGGAAGTCAT CTTTTGTTTC AGATAATAGC 
TTAAACAGGT CTCCTAGATC AGAAAATGAA AAGCCGGGTC TCAGCTCCAG ATCTGTATCC 
ATGATTTCTG TTGCTAGTTC AGAAGATTCC TGCCATACTA CAGTGACAAC CCCAAGGCCT 
CCAGTTGAGT ATGACTCTGA CTTTATGTTA GAGAGTTCAG AATCCCAAAT GTCCTTTTCC 
CAGTCACCTT TTTTGTCAAT TGCCAAATCT CCTGCTCTTC ATGAAAGGGA ATTGGACAGC 
CTGGCTGACT TGCCGGAGCG GATTAAACCA CCATATGCAA ACAGACTTTC AACATCCCAT 
CTTAGGTCAT CTTCTGTAGA AGATGTTAAA CTAATTATAA GCGAGGGGAG ACCTACCATA 
GAAGTTCGAA GATGTAGCAT GCCTTCTGTC ATTTGTGAAC ATACCAAACA ATTCCAAACA 
ATATCAGAAG AGAGCAATCA AGGTAGCTTA TTAACTGTGC CAGGAGATAC TAGTCCTTCT 
CCCAAACCTG AGGTATTCTC AAATGTGCCT GAAAGAGACC TTTCAAATGT ATCTAACATA 
CATTCCAGTT TTGCAACTTC TCCAACTGGA GCTTCAAACA GCAAGTATGT TTCAGCTGAT 
AGAAATCTCA TCAAGAATAC TGCCCCAGTG AACACTGTAA TGGACAGTCC AGTGCATTTA 
GAGCCATCTA GTCAGGTTGG TGTGATCCAG AATAAATCAT GGGAGATGCC TGTTGATAGA 
CTAGAGACAT TAAGCACCAG AGACTTTATC TGCCCAAATT CTAACATACC TGATCAAGAA 
TCCTCTCTTC AGAGTTTTTG TAATTCTGAA AATAAGGTAT TGAAAGAAAA TGCTGATTTT 
TTATCCCTGC GCCAGACTGA ACTGCCAGGA AACTCTTGTG CTCAGGATCC GGCATCCTTT 
ATGCCTCCAC AGCAGCCTTG CTCTTTCCCC AGCCAATCAC TTTCAGATGC TGAATCGATT 
TCTAAACATA TGTCTTTGTC ATATGTTGCT AATCAAGAGC CAGGTATTTT ACAACAAAAA 
AATGCAGTTC AGATTATTAG TTCTGCTTTA GATACTGATA ATGAATCTAC AAAAGATACA 
GAAAATACTT TTGTCCTAGG AGATGTTCAA AAAACAGATG CCTTTGTCCC AGTGTACTCT 
GACAGCACTA TTCAAGAAGC ATCACCAAAC TTTGAGAAAG CTTATACTTT ACCTGTGTTA 
CCATCAGAAA AGGACTTTAA TGGAAGTGAT GCCTCTACCC AGCTAAATAC ACATTATGCA 
TTTAGCAAAC TAACTTACAA GTCTTCCAGT GGCCATGAAG TTGAGAATAG CACAACTGAT 
ACTCAGGTCA TTTCACATGA AAAAGAAAAC AAACTGGAGA GTTTGGTTTT AACTCATTTG 
AGTAGGTGTG ATTCTGATTT ATGTGAAATG AATGCAGGGA TGCCAAAAGG AAACCTAAAT 
GAACAAGATC CAAAACATTG TCCTGAAAGT GAAAAGTGTT TGCTTTCCAT AGAAGATGAG 
GAATCTCAAC AAAGCATTTT ATCAAGTCTG GAAAACCATT CACAGCAGTC AACTCAACCA 
GAAATGCATA AATATGGTCA GTTAGTTAAA GTAGAATTAG AAGAAAATGC CGAAGATGAT 
AAAACTGAAA ACCAAATCCC TCAAAGAATG ACTAGAAACA AAGCAAATAC AATGGCAAAT 
CAAAGCAAAC AGATTCTTGC TAGCTGTACA CTATTATCAG AAAAAGACAG TGAATCCTCA 
TCTCCTAGAG GAAGAATAAG ATTAACTGAA GATGACGATC CTCAAATTCA CCATCCACGG 
AAAAGGAAAG TGTCACGTGT ACCTCAGCCT GTGCAAGTGA GTCCCTCTTT ACTACAAGCA 
AAAGAGAAAA CTCAGCAATC TCTGGCAGCC ATTGTAGATT CTCTAAAACT AGATGAGATT 
CAGCCATACA GTTCAGAGAG AGCAAATCCA TATTTTGAAT ACTTGCACAT AAGGAAAAAA 
ATAGAAGAAA AACGCAAATT ACTGTGTAGT GTGATTCCTC AAGCACCTCA GTACTATGAC 
GAATATGTAA CATTTAACGG ATCATATCTC CTGGATGGAA ACCCCTTAAG CAAGATTTGT 
ATTCCCACAA TTACACCACC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TAAGGATGAA ACTACGTTTG CAACACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAACG AACAAGAAGT TTTGCGAGTT CATTACAGAG CTGCAAGAAC ATTGGCAAAT 
CAAACACTGC CATTTAGTGC TTGTACTGTT TTGCTGGATG CCGAAGTATA CAATGTACCA 
TTGGACTCTC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAA 
TTCATGTCTT GGTTACAAGA TGTGGATGAT AAATTTGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AACATGAAGC TGCGGCTTTA AATGCTGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATTTACGA AATCCAGGAG 
TTTTATGTTC CCCTTGTTGA TGTTAACGAC GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881154.1
CDS	384..6497
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNNSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025665.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025665.1

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA GACAAAAAAG GAAGCTGGAA ATAAGAAATC CACACCAGTT 
AGCATTCTTT TTGGTTATCC ACTCTCTGAG CGAAAACAGA TGGCACTTCT TATGCAGATG 
ACAGCAAGAG ACAACAATTC CACACCAAAT CATCCATCAC AAACAACGCC TGCCCAAAAG 
AAAACTCCCA GTTCTTCATC TCGACAGAAA GATAAAGTTA ATAAAAGAAA TGAACGTGGT 
GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA AACAAGTTAA AGAATTAATA 
AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT GGACACCACT GCATGAAGCT 
TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG CAGCTGGAGC AGATGTTAAC 
ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG CTAGTAGTGG GCACAGAGAT 
ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC AAGCTAATAA ACATGGGGAG 
CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC TACTAAAAAG AGAGGTGCCT 
TTATCTGATG ATGATGAAAG TTACACAGAT TCCGAAGAGG CTCAATCTGT AAATCCTTCT 
AGTGTTGATG AAAATATTGA CTCTGAAACA GAGAAAGACT CTCTCATCTG TGAAAGTAAA 
CAGATACTTC CCAGTAAAAC ACCTCTTCCA TCTGCCCTTG ATGAGTATGA GTTCAAAGAT 
GATGATGATG AAGAAATTAA TAAGATGATT GATGATAGGC ATATTCTTAG GAAAGAACAA 
CGAAAAGAAA ATGAACCTGA AGCAGAAAAA ACTCATTTAT TTGCAAAACA GGAGAAAGCC 
TTCTATCCTA AATCATTTAA AAGTAAAAAA CAAAAGCCAT CTAGGGTCTT ATATTCAAGT 
ACTGAAAGTT CTGATGAAGA AGCTCTTCAG AATAAAAAGA TTTCTACTTC ATGTTCCGTC 
ATCCCTGAAA CATCAAATTC TGATATGCAA ACCAAAAAGG AATATGTAGT TTCAGGTGAA 
CACAAACAGA AAGGCAAAGT TAAAAGAAAA TTGAAAAATC AGAATAAAAA TAAAGAGAAC 
CAAGAGCTAA AGCAAGAAAA GGAAGGAAAA GAAAATACAA GAATAACAAA CTTGACAGTA 
AATACTGGAC TAGATTGTTC AGAAAAGACC AGAGAGGAGG GGAACTTTAG GAAATCTTTT 
AGCCCAAAAG ATGATACTTC ATTACATTTA TTTCATATTT CCACTGGTAA ATCTCCCAAA 
CATTCTTGTG GATTAAGTGA AAAACAGTCA ACACCACTAA AACAAGAACA TACTAAAACA 
TGTTTATCAC CAGGAAGTTC TGAAATGTCA TTACAGCCTG ATCTTGTTCG GTATGATAAT 
ACAGAATCTG AATTCTTGCC AGAAAGTTCA AGTGTAAAAT CTTGTAAGCA TAAGGAAAAA 
AGCAAACATC AGAAAGATTT CCACTTAGAA TTTGGTGAAA AATCAAATGC CAAAATAAAG 
GATGAAGATC ATAGTCCAAC ATTTGAAAAT TCAGATTGCA CACTGAAAAA AATGGATAAA 
GAAGGTAAAA CATTAAAAAA ACATAAATTG AAGCATAAAG AGAGGGAAAA AGAAAAGCAT 
AAAAAAGAAA TTGAAGGTGA AAAGGAAAAA TACAAAACTA AGGATAGTGC CAAAGAACTG 
CAGAGGAGTG TGGAATTTGA TAGAGAATTT TGGAAAGAGA ATTTTTTTAA AAGTGATGAA 
ACTGAAGATC TCTTTTTAAA TATGGAACAT GAATCCTTAA CATTAGAAAA AAAATCAAAA 
TTGGAAAAAA ACATCAAAGA TGATAAATCA ACCAAGGAAA AGCATGTGTC AAAAGAGAGG 
AACTTTAAAG AGGAACGAGA CAAGATTAAA AAGGAAAGCG AGAAATCTTT TAGGGAGGAA 
AAAATAAAAG ATCTAAAAGA AGAGAGAGAA AACATACCCA CAGATAAAGA CTCAGAATTT 
ACTTCTTTGG GTATGAGTGC CATTGAGGAA TCTATAGGGC TTCATTTAGT GGAAAAGGAA 
ATAGACATTG AAAAACAAGA AAAGCATATA AAGGAAAGTA AAGAAAAACC TGAGAAGCGA 
TCTCAAATTA AAGAAAAGGA CATTGAGAAG ATGGAAAGAA AAACCTTTGA AAAAGAAAAG 
AAGATAAAAC ATGAGCATAA GTCAGAAAAA GACAAATTAG ATCTTAGTGA ATGTGTTGAT 
AAAATAAAAG AAAAGGACAA GCTATATTCG CATCACACAG AAAAATGCCA TAAAGAAGGT 
GAGAAGAGCA AAAATACTGC TGCTATTAAA AAAACTGACG ACAGAGAGAA AAGTAGAGAA 
AAGATGGATA GGAAACATGA CAAAGAAAAG CCTGAAAAAG AGAGGCATCT AGCAGAAAGC 
AAAGAAAAGC ACTTGATGGA GAAAAAAAAT AAACAATCAG ATAATAGTGA ATACAGTAAA 
TCAGAAAAAG GCAAAAATAA AGAAAAAGAC AGGGAGCTAG ATAAAAAGGA AAAATCTAGA 
GATAAAGAAA GTATAAATAT AACTAACTCC AAACACATAC AGGAAGAAAA AAAATCAAGT 
ATAGTAGACG GTAATAAAGC ACAACATGAA AAACCCTTAT CCCTTAAAGA AAAAACAAAA 
GATGAACCTT TGAAAACTCC AGATGGAAAA GAAAAAGATA AAAAAGATAA AGATATAGAT 
AGATACAAAG AACGAGACAA ACATAAAGAT AAAATTCAAA TAAATAGCTT ACTCAAACTA 
AAATCTGAAG CAGATAAGCC TAAACCTAAG TCATCACCAG CATCAAAAGA TACCCGACCT 
AAAGAAAAGA GGTTAGTGAA TGATGATTTA ATGCAGACAA GTTTTGAACG AATGCTAAGC 
CTTAAAGACC TAGAAATAGA ACAGTGGCAC AAAAAACATA AGGAAAAAAT TAAGCAAAAA 
GAAAAGGAAC GGTTGAGAAA CCGAAACTGT TTAGAACTTA AAATAAAAGA TAAAGAAAAA 
ACAAAGCATA CACCAACTGA ATCCAAAAAT AAAGAACTTA CTAGGTCAAA GAGTTCAGAA 
GTGACTGATG CATATACCAA GGAGAAACAA CCTAAAGATG CTGTAAGTAA CAGATCACAA 
TCTGTTGACA CCAAAAATGT AATGACTTTA GGGAAGTCAT CTTTTGTTTC AGATAATAGC 
TTAAACAGGT CTCCTAGATC AGAAAATGAA AAGCCGGGTC TCAGCTCCAG ATCTGTATCC 
ATGATTTCTG TTGCTAGTTC AGAAGATTCC TGCCATACTA CAGTGACAAC CCCAAGGCCT 
CCAGTTGAGT ATGACTCTGA CTTTATGTTA GAGAGTTCAG AATCCCAAAT GTCCTTTTCC 
CAGTCACCTT TTTTGTCAAT TGCCAAATCT CCTGCTCTTC ATGAAAGGGA ATTGGACAGC 
CTGGCTGACT TGCCGGAGCG GATTAAACCA CCATATGCAA ACAGACTTTC AACATCCCAT 
CTTAGGTCAT CTTCTGTAGA AGATGTTAAA CTAATTATAA GCGAGGGGAG ACCTACCATA 
GAAGTTCGAA GATGTAGCAT GCCTTCTGTC ATTTGTGAAC ATACCAAACA ATTCCAAACA 
ATATCAGAAG AGAGCAATCA AGGTAGCTTA TTAACTGTGC CAGGAGATAC TAGTCCTTCT 
CCCAAACCTG AGGTATTCTC AAATGTGCCT GAAAGAGACC TTTCAAATGT ATCTAACATA 
CATTCCAGTT TTGCAACTTC TCCAACTGGA GCTTCAAACA GCAAGTATGT TTCAGCTGAT 
AGAAATCTCA TCAAGAATAC TGCCCCAGTG AACACTGTAA TGGACAGTCC AGTGCATTTA 
GAGCCATCTA GTCAGGTTGG TGTGATCCAG AATAAATCAT GGGAGATGCC TGTTGATAGA 
CTAGAGACAT TAAGCACCAG AGACTTTATC TGCCCAAATT CTAACATACC TGATCAAGAA 
TCCTCTCTTC AGAGTTTTTG TAATTCTGAA AATAAGGTAT TGAAAGAAAA TGCTGATTTT 
TTATCCCTGC GCCAGACTGA ACTGCCAGGA AACTCTTGTG CTCAGGATCC GGCATCCTTT 
ATGCCTCCAC AGCAGCCTTG CTCTTTCCCC AGCCAATCAC TTTCAGATGC TGAATCGATT 
TCTAAACATA TGTCTTTGTC ATATGTTGCT AATCAAGAGC CAGGTATTTT ACAACAAAAA 
AATGCAGTTC AGATTATTAG TTCTGCTTTA GATACTGATA ATGAATCTAC AAAAGATACA 
GAAAATACTT TTGTCCTAGG AGATGTTCAA AAAACAGATG CCTTTGTCCC AGTGTACTCT 
GACAGCACTA TTCAAGAAGC ATCACCAAAC TTTGAGAAAG CTTATACTTT ACCTGTGTTA 
CCATCAGAAA AGGACTTTAA TGGAAGTGAT GCCTCTACCC AGCTAAATAC ACATTATGCA 
TTTAGCAAAC TAACTTACAA GTCTTCCAGT GGCCATGAAG TTGAGAATAG CACAACTGAT 
ACTCAGGTCA TTTCACATGA AAAAGAAAAC AAACTGGAGA GTTTGGTTTT AACTCATTTG 
AGTAGGTGTG ATTCTGATTT ATGTGAAATG AATGCAGGGA TGCCAAAAGG AAACCTAAAT 
GAACAAGATC CAAAACATTG TCCTGAAAGT GAAAAGTGTT TGCTTTCCAT AGAAGATGAG 
GAATCTCAAC AAAGCATTTT ATCAAGTCTG GAAAACCATT CACAGCAGTC AACTCAACCA 
GAAATGCATA AATATGGTCA GTTAGTTAAA GTAGAATTAG AAGAAAATGC CGAAGATGAT 
AAAACTGAAA ACCAAATCCC TCAAAGAATG ACTAGAAACA AAGCAAATAC AATGGCAAAT 
CAAAGCAAAC AGATTCTTGC TAGCTGTACA CTATTATCAG AAAAAGACAG TGAATCCTCA 
TCTCCTAGAG GAAGAATAAG ATTAACTGAA GATGACGATC CTCAAATTCA CCATCCACGG 
AAAAGGAAAG TGTCACGTGT ACCTCAGCCT GTGCAAGTGA GTCCCTCTTT ACTACAAGCA 
AAAGAGAAAA CTCAGCAATC TCTGGCAGCC ATTGTAGATT CTCTAAAACT AGATGAGATT 
CAGCCATACA GTTCAGAGAG AGCAAATCCA TATTTTGAAT ACTTGCACAT AAGGAAAAAA 
ATAGAAGAAA AACGCAAATT ACTGTGTAGT GTGATTCCTC AAGCACCTCA GTACTATGAC 
GAATATGTAA CATTTAACGG ATCATATCTC CTGGATGGAA ACCCCTTAAG CAAGATTTGT 
ATTCCCACAA TTACACCACC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TAAGGATGAA ACTACGTTTG CAACACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAACG AACAAGAAGT TTTGCGAGTT CATTACAGAG CTGCAAGAAC ATTGGCAAAT 
CAAACACTGC CATTTAGTGC TTGTACTGTT TTGCTGGATG CCGAAGTATA CAATGTACCA 
TTGGACTCTC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAA 
TTCATGTCTT GGTTACAAGA TGTGGATGAT AAATTTGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AACATGAAGC TGCGGCTTTA AATGCTGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATTTACGA AATCCAGGAG 
TTTTATGTTC CCCTTGTTGA TGTTAACGAC GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025667.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881156.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X12, mRNA.

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CloneID	OHu50998
Clone ID Related Accession (Same CDS sequence)	XM_017025667.1 , XM_011525639.2 , XM_017025668.1 , XM_017025669.1 , XM_017025667.2 , XM_024451109.1 , XM_011525639.3 , XM_017025668.2 , XM_017025669.2
Accession Version	XM_017025667.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881156.1
CDS	1138..6447
Translation	MVNVPELLLGQSPSRNDTKIINSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025667.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025667.1

ORF Insert Sequence:

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_011525639.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523941.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X14, mRNA.

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CloneID	OHu50998
Clone ID Related Accession (Same CDS sequence)	XM_017025667.1 , XM_011525639.2 , XM_017025668.1 , XM_017025669.1 , XM_017025667.2 , XM_024451109.1 , XM_011525639.3 , XM_017025668.2 , XM_017025669.2
Accession Version	XM_011525639.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525639.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523941.1
CDS	1307..6616
Translation	MVNVPELLLGQSPSRNDTKIINSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_011525639.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525639.2

ORF Insert Sequence:

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025668.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881157.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X13, mRNA.

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CloneID	OHu50998
Clone ID Related Accession (Same CDS sequence)	XM_017025667.1 , XM_011525639.2 , XM_017025668.1 , XM_017025669.1 , XM_017025667.2 , XM_024451109.1 , XM_011525639.3 , XM_017025668.2 , XM_017025669.2
Accession Version	XM_017025668.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881157.1
CDS	1132..6441
Translation	MVNVPELLLGQSPSRNDTKIINSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025668.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025668.1

ORF Insert Sequence:

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025669.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881158.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X15, mRNA.

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CloneID	OHu50998
Clone ID Related Accession (Same CDS sequence)	XM_017025667.1 , XM_011525639.2 , XM_017025668.1 , XM_017025669.1 , XM_017025667.2 , XM_024451109.1 , XM_011525639.3 , XM_017025668.2 , XM_017025669.2
Accession Version	XM_017025669.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881158.1
CDS	234..5543
Translation	MVNVPELLLGQSPSRNDTKIINSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025669.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025669.1

ORF Insert Sequence:

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025667.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881156.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X13, mRNA.

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CloneID	OHu50998
Clone ID Related Accession (Same CDS sequence)	XM_017025667.1 , XM_011525639.2 , XM_017025668.1 , XM_017025669.1 , XM_017025667.2 , XM_024451109.1 , XM_011525639.3 , XM_017025668.2 , XM_017025669.2
Accession Version	XM_017025667.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025667.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881156.1
CDS	1163..6472
Translation	MVNVPELLLGQSPSRNDTKIINSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025667.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025667.2

ORF Insert Sequence:

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_024451109.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306877.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X14, mRNA.

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CloneID	OHu50998
Clone ID Related Accession (Same CDS sequence)	XM_017025667.1 , XM_011525639.2 , XM_017025668.1 , XM_017025669.1 , XM_017025667.2 , XM_024451109.1 , XM_011525639.3 , XM_017025668.2 , XM_017025669.2
Accession Version	XM_024451109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306877.1
CDS	1062..6371
Translation	MVNVPELLLGQSPSRNDTKIINSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_024451109.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451109.1

ORF Insert Sequence:

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_011525639.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523941.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X16, mRNA.

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CloneID	OHu50998
Clone ID Related Accession (Same CDS sequence)	XM_017025667.1 , XM_011525639.2 , XM_017025668.1 , XM_017025669.1 , XM_017025667.2 , XM_024451109.1 , XM_011525639.3 , XM_017025668.2 , XM_017025669.2
Accession Version	XM_011525639.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011525639.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523941.1
CDS	1307..6616
Translation	MVNVPELLLGQSPSRNDTKIINSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_011525639.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525639.3

ORF Insert Sequence:

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025668.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881157.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X15, mRNA.

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CloneID	OHu50998
Clone ID Related Accession (Same CDS sequence)	XM_017025667.1 , XM_011525639.2 , XM_017025668.1 , XM_017025669.1 , XM_017025667.2 , XM_024451109.1 , XM_011525639.3 , XM_017025668.2 , XM_017025669.2
Accession Version	XM_017025668.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025668.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881157.1
CDS	1157..6466
Translation	MVNVPELLLGQSPSRNDTKIINSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025668.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025668.2

ORF Insert Sequence:

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025669.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881158.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X17, mRNA.

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CloneID	OHu50998
Clone ID Related Accession (Same CDS sequence)	XM_017025667.1 , XM_011525639.2 , XM_017025668.1 , XM_017025669.1 , XM_017025667.2 , XM_024451109.1 , XM_011525639.3 , XM_017025668.2 , XM_017025669.2
Accession Version	XM_017025669.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025669.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881158.1
CDS	236..5545
Translation	MVNVPELLLGQSPSRNDTKIINSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025669.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025669.2

ORF Insert Sequence:

ATGGTGAATG TGCCTGAATT ATTGCTTGGT CAAAGTCCCT CAAGGAATGA TACAAAAATC 
ATCAATTCCG AAGAGGCTCA ATCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCT 
GAAACAGAGA AAGACTCTCT CATCTGTGAA AGTAAACAGA TACTTCCCAG TAAAACACCT 
CTTCCATCTG CCCTTGATGA GTATGAGTTC AAAGATGATG ATGATGAAGA AATTAATAAG 
ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_011525638.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523940.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X12, mRNA.

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CloneID	OHu65805
Clone ID Related Accession (Same CDS sequence)	XM_011525638.3 , XM_011525638.2
Accession Version	XM_011525638.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011525638.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGTTTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523940.1
CDS	241..6012
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIERVS

Target ORF information:

RefSeq Version	XM_011525638.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525638.3

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGTTTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_011525638.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523940.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X11, mRNA.

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CloneID	OHu65805
Clone ID Related Accession (Same CDS sequence)	XM_011525638.3 , XM_011525638.2
Accession Version	XM_011525638.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGTTTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523940.1
CDS	237..6008
Translation	MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDEYEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIERVS

Target ORF information:

RefSeq Version	XM_011525638.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525638.2

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAATT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAGA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA CAGCAAAACT 
CCAAAAATTG AACGAAGTGA TGTGAGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACTATTAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GATCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAG ACAAAAAAGG AAGCTGGAAA TAAGAAATCC 
ACACCAGTTA GCATTCTTTT TGGTTATCCA CTCTCTGAGC GAAAACAGAT GGCACTTCTT 
ATGCAGATGA CAGCAAGAGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCACAAACA 
ACGCCTGCCC AAAAGAAAAC TCCCAGTTCT TCATCTCGAC AGAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCTGCTA TTCGAGGAGA TGTGAAACAA 
GTTAAAGAAT TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA TGTTGGATAT TACGATGTTG CTAAGATACT TATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCTGCTAGT 
AGTGGGCACA GAGATATAGT AAAGCTGTTA CTTCGTCACG GTGGAAATCC ATTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTGGATGTA GCAGAAACAG AGGAGTTGGA GTTGCTACTA 
AAAAGAGAGG TGCCTTTATC TGATGATGAT GAAAGTTACA CAGATTCCGA AGAGGCTCAA 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCTG AAACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT ACTTCCCAGT AAAACACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGATGAAGAA ATTAATAAGA TGATTGATGA TAGGCATATT 
CTTAGGAAAG AACAACGAAA AGAAAATGAA CCTGAAGCAG AAAAAACTCA TTTATTTGCA 
AAACAGGAGA AAGCCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTCTTATATT CAAGTACTGA AAGTTCTGAT GAAGAAGCTC TTCAGAATAA AAAGATTTCT 
ACTTCATGTT CCGTCATCCC TGAAACATCA AATTCTGATA TGCAAACCAA AAAGGAATAT 
GTAGTTTCAG GTGAACACAA ACAGAAAGGC AAAGTTAAAA GAAAATTGAA AAATCAGAAT 
AAAAATAAAG AGAACCAAGA GCTAAAGCAA GAAAAGGAAG GAAAAGAAAA TACAAGAATA 
ACAAACTTGA CAGTAAATAC TGGACTAGAT TGTTCAGAAA AGACCAGAGA GGAGGGGAAC 
TTTAGGAAAT CTTTTAGCCC AAAAGATGAT ACTTCATTAC ATTTATTTCA TATTTCCACT 
GGTAAATCTC CCAAACATTC TTGTGGATTA AGTGAAAAAC AGTCAACACC ACTAAAACAA 
GAACATACTA AAACATGTTT ATCACCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTT 
GTTCGGTATG ATAATACAGA ATCTGAATTC TTGCCAGAAA GTTCAAGTGT AAAATCTTGT 
AAGCATAAGG AAAAAAGCAA ACATCAGAAA GATTTCCACT TAGAATTTGG TGAAAAATCA 
AATGCCAAAA TAAAGGATGA AGATCATAGT CCAACATTTG AAAATTCAGA TTGCACACTG 
AAAAAAATGG ATAAAGAAGG TAAAACATTA AAAAAACATA AATTGAAGCA TAAAGAGAGG 
GAAAAAGAAA AGCATAAAAA AGAAATTGAA GGTGAAAAGG AAAAATACAA AACTAAGGAT 
AGTGCCAAAG AACTGCAGAG GAGTGTGGAA TTTGATAGAG AATTTTGGAA AGAGAATTTT 
TTTAAAAGTG ATGAAACTGA AGATCTCTTT TTAAATATGG AACATGAATC CTTAACATTA 
GAAAAAAAAT CAAAATTGGA AAAAAACATC AAAGATGATA AATCAACCAA GGAAAAGCAT 
GTGTCAAAAG AGAGGAACTT TAAAGAGGAA CGAGACAAGA TTAAAAAGGA AAGCGAGAAA 
TCTTTTAGGG AGGAAAAAAT AAAAGATCTA AAAGAAGAGA GAGAAAACAT ACCCACAGAT 
AAAGACTCAG AATTTACTTC TTTGGGTATG AGTGCCATTG AGGAATCTAT AGGGCTTCAT 
TTAGTGGAAA AGGAAATAGA CATTGAAAAA CAAGAAAAGC ATATAAAGGA AAGTAAAGAA 
AAACCTGAGA AGCGATCTCA AATTAAAGAA AAGGACATTG AGAAGATGGA AAGAAAAACC 
TTTGAAAAAG AAAAGAAGAT AAAACATGAG CATAAGTCAG AAAAAGACAA ATTAGATCTT 
AGTGAATGTG TTGATAAAAT AAAAGAAAAG GACAAGCTAT ATTCGCATCA CACAGAAAAA 
TGCCATAAAG AAGGTGAGAA GAGCAAAAAT ACTGCTGCTA TTAAAAAAAC TGACGACAGA 
GAGAAAAGTA GAGAAAAGAT GGATAGGAAA CATGACAAAG AAAAGCCTGA AAAAGAGAGG 
CATCTAGCAG AAAGCAAAGA AAAGCACTTG ATGGAGAAAA AAAATAAACA ATCAGATAAT 
AGTGAATACA GTAAATCAGA AAAAGGCAAA AATAAAGAAA AAGACAGGGA GCTAGATAAA 
AAGGAAAAAT CTAGAGATAA AGAAAGTATA AATATAACTA ACTCCAAACA CATACAGGAA 
GAAAAAAAAT CAAGTATAGT AGACGGTAAT AAAGCACAAC ATGAAAAACC CTTATCCCTT 
AAAGAAAAAA CAAAAGATGA ACCTTTGAAA ACTCCAGATG GAAAAGAAAA AGATAAAAAA 
GATAAAGATA TAGATAGATA CAAAGAACGA GACAAACATA AAGATAAAAT TCAAATAAAT 
AGCTTACTCA AACTAAAATC TGAAGCAGAT AAGCCTAAAC CTAAGTCATC ACCAGCATCA 
AAAGATACCC GACCTAAAGA AAAGAGGTTA GTGAATGATG ATTTAATGCA GACAAGTTTT 
GAACGAATGC TAAGCCTTAA AGACCTAGAA ATAGAACAGT GGCACAAAAA ACATAAGGAA 
AAAATTAAGC AAAAAGAAAA GGAACGGTTG AGAAACCGAA ACTGTTTAGA ACTTAAAATA 
AAAGATAAAG AAAAAACAAA GCATACACCA ACTGAATCCA AAAATAAAGA ACTTACTAGG 
TCAAAGAGTT CAGAAGTGAC TGATGCATAT ACCAAGGAGA AACAACCTAA AGATGCTGTA 
AGTAACAGAT CACAATCTGT TGACACCAAA AATGTAATGA CTTTAGGGAA GTCATCTTTT 
GTTTCAGATA ATAGCTTAAA CAGGTCTCCT AGATCAGAAA ATGAAAAGCC GGGTCTCAGC 
TCCAGATCTG TATCCATGAT TTCTGTTGCT AGTTCAGAAG ATTCCTGCCA TACTACAGTG 
ACAACCCCAA GGCCTCCAGT TGAGTATGAC TCTGACTTTA TGTTAGAGAG TTCAGAATCC 
CAAATGTCCT TTTCCCAGTC ACCTTTTTTG TCAATTGCCA AATCTCCTGC TCTTCATGAA 
AGGGAATTGG ACAGCCTGGC TGACTTGCCG GAGCGGATTA AACCACCATA TGCAAACAGA 
CTTTCAACAT CCCATCTTAG GTCATCTTCT GTAGAAGATG TTAAACTAAT TATAAGCGAG 
GGGAGACCTA CCATAGAAGT TCGAAGATGT AGCATGCCTT CTGTCATTTG TGAACATACC 
AAACAATTCC AAACAATATC AGAAGAGAGC AATCAAGGTA GCTTATTAAC TGTGCCAGGA 
GATACTAGTC CTTCTCCCAA ACCTGAGGTA TTCTCAAATG TGCCTGAAAG AGACCTTTCA 
AATGTATCTA ACATACATTC CAGTTTTGCA ACTTCTCCAA CTGGAGCTTC AAACAGCAAG 
TATGTTTCAG CTGATAGAAA TCTCATCAAG AATACTGCCC CAGTGAACAC TGTAATGGAC 
AGTCCAGTGC ATTTAGAGCC ATCTAGTCAG GTTGGTGTGA TCCAGAATAA ATCATGGGAG 
ATGCCTGTTG ATAGACTAGA GACATTAAGC ACCAGAGACT TTATCTGCCC AAATTCTAAC 
ATACCTGATC AAGAATCCTC TCTTCAGAGT TTTTGTAATT CTGAAAATAA GGTATTGAAA 
GAAAATGCTG ATTTTTTATC CCTGCGCCAG ACTGAACTGC CAGGAAACTC TTGTGCTCAG 
GATCCGGCAT CCTTTATGCC TCCACAGCAG CCTTGCTCTT TCCCCAGCCA ATCACTTTCA 
GATGCTGAAT CGATTTCTAA ACATATGTCT TTGTCATATG TTGCTAATCA AGAGCCAGGT 
ATTTTACAAC AAAAAAATGC AGTTCAGATT ATTAGTTCTG CTTTAGATAC TGATAATGAA 
TCTACAAAAG ATACAGAAAA TACTTTTGTC CTAGGAGATG TTCAAAAAAC AGATGCCTTT 
GTCCCAGTGT ACTCTGACAG CACTATTCAA GAAGCATCAC CAAACTTTGA GAAAGCTTAT 
ACTTTACCTG TGTTACCATC AGAAAAGGAC TTTAATGGAA GTGATGCCTC TACCCAGCTA 
AATACACATT ATGCATTTAG CAAACTAACT TACAAGTCTT CCAGTGGCCA TGAAGTTGAG 
AATAGCACAA CTGATACTCA GGTCATTTCA CATGAAAAAG AAAACAAACT GGAGAGTTTG 
GTTTTAACTC ATTTGAGTAG GTGTGATTCT GATTTATGTG AAATGAATGC AGGGATGCCA 
AAAGGAAACC TAAATGAACA AGATCCAAAA CATTGTCCTG AAAGTGAAAA GTGTTTGCTT 
TCCATAGAAG ATGAGGAATC TCAACAAAGC ATTTTATCAA GTCTGGAAAA CCATTCACAG 
CAGTCAACTC AACCAGAAAT GCATAAATAT GGTCAGTTAG TTAAAGTAGA ATTAGAAGAA 
AATGCCGAAG ATGATAAAAC TGAAAACCAA ATCCCTCAAA GAATGACTAG AAACAAAGCA 
AATACAATGG CAAATCAAAG CAAACAGATT CTTGCTAGCT GTACACTATT ATCAGAAAAA 
GACAGTGAAT CCTCATCTCC TAGAGGAAGA ATAAGATTAA CTGAAGATGA CGATCCTCAA 
ATTCACCATC CACGGAAAAG GAAAGTGTCA CGTGTACCTC AGCCTGTGCA AGTGAGTCCC 
TCTTTACTAC AAGCAAAAGA GAAAACTCAG CAATCTCTGG CAGCCATTGT AGATTCTCTA 
AAACTAGATG AGATTCAGCC ATACAGTTCA GAGAGAGCAA ATCCATATTT TGAATACTTG 
CACATAAGGA AAAAAATAGA AGAAAAACGC AAATTACTGT GTAGTGTGAT TCCTCAAGCA 
CCTCAGTACT ATGACGAATA TGTAACATTT AACGGATCAT ATCTCCTGGA TGGAAACCCC 
TTAAGCAAGA TTTGTATTCC CACAATTACA CCACCACCTT CACTGTCAGA TCCACTTAAA 
GAGCTTTTTC GACAACAGGA AGTTGTAAGG ATGAAACTAC GTTTGCAACA CAGTATTGAA 
AGGGTTTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025670.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881159.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X18, mRNA.

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CloneID	OHu87827
Clone ID Related Accession (Same CDS sequence)	XM_017025670.2 , XM_017025670.1
Accession Version	XM_017025670.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025670.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881159.1
CDS	941..6010
Translation	MIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025670.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025670.2

ORF Insert Sequence:

ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_017025670.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881159.1 Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X16, mRNA.

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CloneID	OHu87827
Clone ID Related Accession (Same CDS sequence)	XM_017025670.2 , XM_017025670.1
Accession Version	XM_017025670.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 12 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881159.1
CDS	941..6010
Translation	MIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSRVLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTLEKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTDKDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKTFEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDREKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTRSKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLSSRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHERELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHTKQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSKYVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSNIPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLSDAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAFVPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVENSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLLSIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_017025670.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025670.1

ORF Insert Sequence:

ATGATTGATG ATAGGCATAT TCTTAGGAAA GAACAACGAA AAGAAAATGA ACCTGAAGCA 
GAAAAAACTC ATTTATTTGC AAAACAGGAG AAAGCCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTCTTATAT TCAAGTACTG AAAGTTCTGA TGAAGAAGCT 
CTTCAGAATA AAAAGATTTC TACTTCATGT TCCGTCATCC CTGAAACATC AAATTCTGAT 
ATGCAAACCA AAAAGGAATA TGTAGTTTCA GGTGAACACA AACAGAAAGG CAAAGTTAAA 
AGAAAATTGA AAAATCAGAA TAAAAATAAA GAGAACCAAG AGCTAAAGCA AGAAAAGGAA 
GGAAAAGAAA ATACAAGAAT AACAAACTTG ACAGTAAATA CTGGACTAGA TTGTTCAGAA 
AAGACCAGAG AGGAGGGGAA CTTTAGGAAA TCTTTTAGCC CAAAAGATGA TACTTCATTA 
CATTTATTTC ATATTTCCAC TGGTAAATCT CCCAAACATT CTTGTGGATT AAGTGAAAAA 
CAGTCAACAC CACTAAAACA AGAACATACT AAAACATGTT TATCACCAGG AAGTTCTGAA 
ATGTCATTAC AGCCTGATCT TGTTCGGTAT GATAATACAG AATCTGAATT CTTGCCAGAA 
AGTTCAAGTG TAAAATCTTG TAAGCATAAG GAAAAAAGCA AACATCAGAA AGATTTCCAC 
TTAGAATTTG GTGAAAAATC AAATGCCAAA ATAAAGGATG AAGATCATAG TCCAACATTT 
GAAAATTCAG ATTGCACACT GAAAAAAATG GATAAAGAAG GTAAAACATT AAAAAAACAT 
AAATTGAAGC ATAAAGAGAG GGAAAAAGAA AAGCATAAAA AAGAAATTGA AGGTGAAAAG 
GAAAAATACA AAACTAAGGA TAGTGCCAAA GAACTGCAGA GGAGTGTGGA ATTTGATAGA 
GAATTTTGGA AAGAGAATTT TTTTAAAAGT GATGAAACTG AAGATCTCTT TTTAAATATG 
GAACATGAAT CCTTAACATT AGAAAAAAAA TCAAAATTGG AAAAAAACAT CAAAGATGAT 
AAATCAACCA AGGAAAAGCA TGTGTCAAAA GAGAGGAACT TTAAAGAGGA ACGAGACAAG 
ATTAAAAAGG AAAGCGAGAA ATCTTTTAGG GAGGAAAAAA TAAAAGATCT AAAAGAAGAG 
AGAGAAAACA TACCCACAGA TAAAGACTCA GAATTTACTT CTTTGGGTAT GAGTGCCATT 
GAGGAATCTA TAGGGCTTCA TTTAGTGGAA AAGGAAATAG ACATTGAAAA ACAAGAAAAG 
CATATAAAGG AAAGTAAAGA AAAACCTGAG AAGCGATCTC AAATTAAAGA AAAGGACATT 
GAGAAGATGG AAAGAAAAAC CTTTGAAAAA GAAAAGAAGA TAAAACATGA GCATAAGTCA 
GAAAAAGACA AATTAGATCT TAGTGAATGT GTTGATAAAA TAAAAGAAAA GGACAAGCTA 
TATTCGCATC ACACAGAAAA ATGCCATAAA GAAGGTGAGA AGAGCAAAAA TACTGCTGCT 
ATTAAAAAAA CTGACGACAG AGAGAAAAGT AGAGAAAAGA TGGATAGGAA ACATGACAAA 
GAAAAGCCTG AAAAAGAGAG GCATCTAGCA GAAAGCAAAG AAAAGCACTT GATGGAGAAA 
AAAAATAAAC AATCAGATAA TAGTGAATAC AGTAAATCAG AAAAAGGCAA AAATAAAGAA 
AAAGACAGGG AGCTAGATAA AAAGGAAAAA TCTAGAGATA AAGAAAGTAT AAATATAACT 
AACTCCAAAC ACATACAGGA AGAAAAAAAA TCAAGTATAG TAGACGGTAA TAAAGCACAA 
CATGAAAAAC CCTTATCCCT TAAAGAAAAA ACAAAAGATG AACCTTTGAA AACTCCAGAT 
GGAAAAGAAA AAGATAAAAA AGATAAAGAT ATAGATAGAT ACAAAGAACG AGACAAACAT 
AAAGATAAAA TTCAAATAAA TAGCTTACTC AAACTAAAAT CTGAAGCAGA TAAGCCTAAA 
CCTAAGTCAT CACCAGCATC AAAAGATACC CGACCTAAAG AAAAGAGGTT AGTGAATGAT 
GATTTAATGC AGACAAGTTT TGAACGAATG CTAAGCCTTA AAGACCTAGA AATAGAACAG 
TGGCACAAAA AACATAAGGA AAAAATTAAG CAAAAAGAAA AGGAACGGTT GAGAAACCGA 
AACTGTTTAG AACTTAAAAT AAAAGATAAA GAAAAAACAA AGCATACACC AACTGAATCC 
AAAAATAAAG AACTTACTAG GTCAAAGAGT TCAGAAGTGA CTGATGCATA TACCAAGGAG 
AAACAACCTA AAGATGCTGT AAGTAACAGA TCACAATCTG TTGACACCAA AAATGTAATG 
ACTTTAGGGA AGTCATCTTT TGTTTCAGAT AATAGCTTAA ACAGGTCTCC TAGATCAGAA 
AATGAAAAGC CGGGTCTCAG CTCCAGATCT GTATCCATGA TTTCTGTTGC TAGTTCAGAA 
GATTCCTGCC ATACTACAGT GACAACCCCA AGGCCTCCAG TTGAGTATGA CTCTGACTTT 
ATGTTAGAGA GTTCAGAATC CCAAATGTCC TTTTCCCAGT CACCTTTTTT GTCAATTGCC 
AAATCTCCTG CTCTTCATGA AAGGGAATTG GACAGCCTGG CTGACTTGCC GGAGCGGATT 
AAACCACCAT ATGCAAACAG ACTTTCAACA TCCCATCTTA GGTCATCTTC TGTAGAAGAT 
GTTAAACTAA TTATAAGCGA GGGGAGACCT ACCATAGAAG TTCGAAGATG TAGCATGCCT 
TCTGTCATTT GTGAACATAC CAAACAATTC CAAACAATAT CAGAAGAGAG CAATCAAGGT 
AGCTTATTAA CTGTGCCAGG AGATACTAGT CCTTCTCCCA AACCTGAGGT ATTCTCAAAT 
GTGCCTGAAA GAGACCTTTC AAATGTATCT AACATACATT CCAGTTTTGC AACTTCTCCA 
ACTGGAGCTT CAAACAGCAA GTATGTTTCA GCTGATAGAA ATCTCATCAA GAATACTGCC 
CCAGTGAACA CTGTAATGGA CAGTCCAGTG CATTTAGAGC CATCTAGTCA GGTTGGTGTG 
ATCCAGAATA AATCATGGGA GATGCCTGTT GATAGACTAG AGACATTAAG CACCAGAGAC 
TTTATCTGCC CAAATTCTAA CATACCTGAT CAAGAATCCT CTCTTCAGAG TTTTTGTAAT 
TCTGAAAATA AGGTATTGAA AGAAAATGCT GATTTTTTAT CCCTGCGCCA GACTGAACTG 
CCAGGAAACT CTTGTGCTCA GGATCCGGCA TCCTTTATGC CTCCACAGCA GCCTTGCTCT 
TTCCCCAGCC AATCACTTTC AGATGCTGAA TCGATTTCTA AACATATGTC TTTGTCATAT 
GTTGCTAATC AAGAGCCAGG TATTTTACAA CAAAAAAATG CAGTTCAGAT TATTAGTTCT 
GCTTTAGATA CTGATAATGA ATCTACAAAA GATACAGAAA ATACTTTTGT CCTAGGAGAT 
GTTCAAAAAA CAGATGCCTT TGTCCCAGTG TACTCTGACA GCACTATTCA AGAAGCATCA 
CCAAACTTTG AGAAAGCTTA TACTTTACCT GTGTTACCAT CAGAAAAGGA CTTTAATGGA 
AGTGATGCCT CTACCCAGCT AAATACACAT TATGCATTTA GCAAACTAAC TTACAAGTCT 
TCCAGTGGCC ATGAAGTTGA GAATAGCACA ACTGATACTC AGGTCATTTC ACATGAAAAA 
GAAAACAAAC TGGAGAGTTT GGTTTTAACT CATTTGAGTA GGTGTGATTC TGATTTATGT 
GAAATGAATG CAGGGATGCC AAAAGGAAAC CTAAATGAAC AAGATCCAAA ACATTGTCCT 
GAAAGTGAAA AGTGTTTGCT TTCCATAGAA GATGAGGAAT CTCAACAAAG CATTTTATCA 
AGTCTGGAAA ACCATTCACA GCAGTCAACT CAACCAGAAA TGCATAAATA TGGTCAGTTA 
GTTAAAGTAG AATTAGAAGA AAATGCCGAA GATGATAAAA CTGAAAACCA AATCCCTCAA 
AGAATGACTA GAAACAAAGC AAATACAATG GCAAATCAAA GCAAACAGAT TCTTGCTAGC 
TGTACACTAT TATCAGAAAA AGACAGTGAA TCCTCATCTC CTAGAGGAAG AATAAGATTA 
ACTGAAGATG ACGATCCTCA AATTCACCAT CCACGGAAAA GGAAAGTGTC ACGTGTACCT 
CAGCCTGTGC AAGTGAGTCC CTCTTTACTA CAAGCAAAAG AGAAAACTCA GCAATCTCTG 
GCAGCCATTG TAGATTCTCT AAAACTAGAT GAGATTCAGC CATACAGTTC AGAGAGAGCA 
AATCCATATT TTGAATACTT GCACATAAGG AAAAAAATAG AAGAAAAACG CAAATTACTG 
TGTAGTGTGA TTCCTCAAGC ACCTCAGTAC TATGACGAAT ATGTAACATT TAACGGATCA 
TATCTCCTGG ATGGAAACCC CTTAAGCAAG ATTTGTATTC CCACAATTAC ACCACCACCT 
TCACTGTCAG ATCCACTTAA AGAGCTTTTT CGACAACAGG AAGTTGTAAG GATGAAACTA 
CGTTTGCAAC ACAGTATTGA AAGGGAAAAA CTCATTGTAT CCAACGAACA AGAAGTTTTG 
CGAGTTCATT ACAGAGCTGC AAGAACATTG GCAAATCAAA CACTGCCATT TAGTGCTTGT 
ACTGTTTTGC TGGATGCCGA AGTATACAAT GTACCATTGG ACTCTCAGTC TGATGACAGT 
AAAACTTCTG TGAGGGATCG CTTTAATGCA AGACAATTCA TGTCTTGGTT ACAAGATGTG 
GATGATAAAT TTGACAAATT AAAGACCTGT CTTTTAATGA GGCAACAACA TGAAGCTGCG 
GCTTTAAATG CTGTCCAGAG GTTAGAATGG CAGCTCAAAC TCCAGGAACT TGATCCTGCC 
ACCTATAAAT CTATCAGCAT TTACGAAATC CAGGAGTTTT ATGTTCCCCT TGTTGATGTT 
AACGACGACT TTGAATTGAC TCCTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Clinical significance of Ankyrin repeat domain 12 expression in colorectal cancer.
Journal of experimental & clinical cancer research : CR32(1)35(2013 May)
Bai R,Li D,Shi Z,Fang X,Ge W,Zheng S

Ankyrin repeats-containing cofactors interact with ADA3 and modulate its co-activator function.
The Biochemical journal413(2)349-57(2008 Jul)
Li CW,Dinh GK,Zhang A,Chen JD

A germline-specific class of small RNAs binds mammalian Piwi proteins.
Nature442(7099)199-202(2006 Jul)
Girard A,Sachidanandam R,Hannon GJ,Carmell MA

Identification of novel ARF binding proteins by two-hybrid screening.
Cell cycle (Georgetown, Tex.)5(6)641-6(2006 Mar)
Tompkins V,Hagen J,Zediak VP,Quelle DE

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オンライン注文

ANKRD12 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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