Ankrd12 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_030249418.1	XP_030105278.1	ankyrin repeat domain-containing protein 12 isoform X3
XM_006523399.4	XP_006523462.1	ankyrin repeat domain-containing protein 12 isoform X5
XM_006523395.3	XP_006523458.1	ankyrin repeat domain-containing protein 12 isoform X1
XM_006523395.4	XP_006523458.1	ankyrin repeat domain-containing protein 12 isoform X1
XM_011246238.2	XP_011244540.1	ankyrin repeat domain-containing protein 12 isoform X5
XM_006523397.4	XP_006523460.1	ankyrin repeat domain-containing protein 12 isoform X3
XM_030249417.1	XP_030105277.1	ankyrin repeat domain-containing protein 12 isoform X3
XM_030249419.1	XP_030105279.1	ankyrin repeat domain-containing protein 12 isoform X4
XM_006523394.3	XP_006523457.1	ankyrin repeat domain-containing protein 12 isoform X1
XM_006523393.4	XP_006523456.1	ankyrin repeat domain-containing protein 12 isoform X1
XM_006523394.4	XP_006523457.1	ankyrin repeat domain-containing protein 12 isoform X1
NM_001025572.1	NP_001020743.1	ankyrin repeat domain-containing protein 12
XM_006523393.3	XP_006523456.1	ankyrin repeat domain-containing protein 12 isoform X1
XM_006523396.3	XP_006523459.1	ankyrin repeat domain-containing protein 12 isoform X2
XM_006523397.3	XP_006523460.1	ankyrin repeat domain-containing protein 12 isoform X3
XM_006523399.3	XP_006523462.1	ankyrin repeat domain-containing protein 12 isoform X4
XM_006523396.4	XP_006523459.1	ankyrin repeat domain-containing protein 12 isoform X2
XM_030249420.1	XP_030105280.1	ankyrin repeat domain-containing protein 12 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu72156	XM_030249420.1 Latest version!	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu72156	XM_011246238.2 Latest version!	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu52953	XM_006523393.4 Latest version!	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu52953	XM_006523395.4 Latest version!	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu52953	XM_006523394.4 Latest version!	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu52954	XM_006523396.4 Latest version!	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu52955	XM_030249417.1 Latest version!	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu52955	XM_030249418.1 Latest version!	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu52955	XM_006523397.4 Latest version!	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu52957	XM_006523399.4 Latest version!	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu87054	XM_030249419.1 Latest version!	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu12207	NM_001025572.1 Latest version!	Mus musculus ankyrin repeat domain 12 (Ankrd12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu52953	XM_006523395.3	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu52953	XM_006523394.3	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu52953	XM_006523393.3	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu52955	XM_006523397.3	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu52957	XM_006523399.3	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu52954	XM_006523396.3	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

Ankrd12 ( XM_030249420.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105280.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu72156
Clone ID Related Accession (Same CDS sequence)	XM_011246238.2 , XM_030249420.1
Accession Version	XM_030249420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG TGCCTGAATT ATTGCTTGAT CAAAGTGCGT CAAGGAATGA TGCAAAAATT 
ATCAATTCAG AAGAGGCTCA GTCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCA 
GAGACAGAGA AAGACTCTTT CGTCTGTGGC AGTAAAATCC TCCCAAGCAA AGCCCCGCTG 
CCTTCTGCAC TGGATGAGTA TGAGTTCAAA GATGATGATG AAGAAGAGAT AAGTAAGATG 
ATTGACGACA GGCACATTCT TAGGAAAGAA CAGCGGAGAG AGAATGAATC TGAAGCAGAA 
AGGAGTGGTC TGTTTGCAAA ACAGGAGAAA ACCTTCTATT CTAAGTCATT TAAAACAAAA 
AAACAGAAGC CGTCAAGGGT TCTGTGCTCC AGTACTGAAA GCTCTGATGA AGAGGGTCTT 
CAGAACAGAA AGATTTCTAC TGCTTGTTCT GTTGCTGAAT CATCACATCC TGATATGCAA 
GCCAAAAAAG AATATGAATA CAAACAGAAA GGCAAAGTTA AAAGAAAATT AAAAAATCAG 
AATAAAAACA AAGAGAACCA AGAGCTGAAA CAAGAAAAAG AGGGGAAAGA AAATAGCAGG 
ATAGCAACCT TGACAGTTAA TACTGCGCTG GATTGTTCAG AAAAATACAG AGAGGAGGGG 
AACTTCAGGA AATCTTTTAG CCCAAAGGAT GATACTTCAT TACACTTATT TCATATTTCC 
ACTGGTAAAT CTCCCAAACA TTCCTGTGGA TTAAGTGAAA AACAGACACC ACTGAAGCAA 
GAACACACGA AAGCATGCAT ATCCCCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTC 
ACTCGGTATG ATAATGCAGA ACCTGAAAGT TCAAATGTCA AATCTTATAA GCACAAGGAA 
AAAAACAAAC AGCAGAAAGA TTTTCACATA GAGTTTGGTG AAAAATCAAA TGCCAAAATT 
AAAGATGAAG ATCATAATCC AGCATTTGAA AACTCAGACT GCACACTGAA GAAGATGGAT 
AAAGAGGGTA AAACATTAAA AAAACATAAA TTAAAACATA AAGAGAAGGA TAAAGAAAAG 
CACAAAAAGG AAGTTGAAGG TGAAAAGGAA AAATATAAAA ATAAAGACAA TGCTAAAGAG 
CTACAGAGGA GCATCGAATT TGACAGAGAG TTTTGGAAAG AAAATTTTTT TAAAAGTGAT 
GAAACTGAGG ATCTCTTTTT AACTATGGAA CATGAGTCCA TAATAGAAAA AAAGACAAAA 
ATGGAAAAAA ACATGAAAGA TGATAGGTCA GCTAAAGAAA AGCATGTCTC AAAAGAAAGG 
AACTTTAAAG AAGAACGGGA AAAGACTAAA AAGGAAAATG AAAAATCTCT TAGGGAAGAA 
AAGGTAAAAG ATCCGAAAGA AGAAAAAGAC AGTGTATCCA CAGAGAAGGA GGCCGAGTGC 
TGTTCGGTGG GTGTCAGTGT GAGCCAGGAA GCTGCAGGGC TGCATTCATC AGAAAAGGAA 
ATGGATGCTG AGAAGCAAGA AAGACACACA AAAGAGAGGG AAAAGTCTGA TCGGCGCTTT 
CAGACTAAGG AGAAAGAGCT TGACAGGAAC GAGAGGAAAT TTTCTGAAAA AGAAAAGAAG 
ATAAAACATG AACATAAGTC AGAAAGAGAG AAGCTGGATT TCAGTGACTG CACTGACAGA 
GTGAGAGAGA AGGACAAGCT GTACTCACAC CAGACAGAAA GGTGCCACAA GGACAGCGAG 
AAGCTGAAAA ACACTGTCAG AAAGACTGAT GACAGAGAGA GGAACAGAGA AAAGGCGGAT 
AGAAAGCACG ACAGAGAGAA ACCTGAACGA GAGAGGTACT TAGCAGAAAA CAAAGAAAAG 
CACTTAGAGA AAAGAAAGCA ATCTGACAGC AGTGAGTATT CCAAATCAGA GAAGGTCAGA 
ACTAAAGACA GAGACAGGGA AGGAGAGAAG AAGGAGAAAC TTAGAGATAA AGAAAGTGTA 
AATGTAACTA ACTTGAAACA TTTGCAGGAA GAGAGAAAGT CCAGTACAGG AGACAGCAGT 
GGTAAAACCC AGCATGAGAA AGCTCTGTCC CTTAAAGAAA GGGCAAGAGA TGAGCCTTTG 
AAAACTCCAG ATGGAAAAGA GAAAGATAAA AAGGATATAG ACAGATACAA AGAGCGAGAC 
AAGCGTAAAG ATAAAGCCCA GCTAAATACT TTAATCAGAC TAAAATCTGA AACAGAGAAG 
CTTAAACCTA AATTATCACC AGCATCAAAA GATACTCGAC CTAAAGAAAA GAGGTTAGTA 
AATGATGATT TAATGCAGAC AAGTTTTGAA CGAATGCTTA GCCTTAAAGA CCTAGAAATA 
GAACAGTGGC ACAGAAAACA TAAGGAAAAA ATTAAGCAAA AGGAAAAAGA ACGATTACGC 
AATCGTACTT GTTTAGAACT TAAAGTAAAA GATAAAGAAA AAACAAAACA TGCACCCACA 
GAATCCAAAA GTAAAGAACT TACCAGGTCA AGAAGTTCAG AGTTGACTGA TGTTTATAGC 
AAGGAGAAAC AATCTAAAGA TGTTGGCAGT AATCGCTCAC AATCTGTTGA CAACAGAAAT 
GTATTGAATT TAGGAAAGCC ATCCTACATT TCAGAGAGTG GCTCCAACCG GTCTCCTAGG 
TCGGAAGGTG AGAGGCTGGG TCTTAGCTCT AGGTCTGTGT CCATGCTTTC TGTTGCTAGT 
TCAGAAGACT CTTGCCATAC AGTGACAACC CCAAGGCCTC TCGTTGAGTA TGACTCTGAC 
TTTATGTTAG AGGGCTCGGA ATCTCAGTTG TCATTTTCTC AGTCACCTTT TTTGCCAAAT 
GCCAAATCTC CAGCTCTTTA TGAAAGAGAG CTGGAGGGTC TCACTGAACT ACCAGACCGA 
CTTAAACCAC CATATACAAA CAGACTTCCA GTGTGCCATC TCAGGTCATC TTCTGTGGAA 
GATGTTAAAC TAATTACTAA TGAGGTAAGA CCTGCTGTAG AAATTAGAAG ATGCAGTATG 
CCCTCAGTTA TCTGTGAGCC CATAAAACAT TTCCAAGTAT CAGAAGAAAG CAGTCAAGGC 
AACTTAGCTG TTCCCAGAGA GACCTGTCTG TCTCCCAAGC CTGAAGTGTC TTCAGCAGTA 
CCTGAGAGGG GCCTTTCGCA TGTGTCTGAC TTACATCCTA ACTGTACAGG CTCTCCAACG 
AGGTCTGCAA ACAGCAGCTA TATTTCATCT GATGTAAATG TAATCAAGAG TGCAACTCTT 
GTGGGTACAC TGATTGACAG TTCGATGCAT TTGGAACCGT CTAATCAGGT TGGAGTGATT 
CCAAGTAAAT CATGGGAGAC GCCTGTTGAT AAACTGGAGT CTTTAAGCAC CAATGACTTC 
ATCTGTCCAG ATTCTAATAT ATCTGATCAA GATTCCTCTG CTCAGAGTTT TTATAATTCA 
GAAAACAAAA TGTTAAGAGA ACAAAATACT GACTGCTTAC CTCCGCACCA GCCTGAACTG 
CCAGGAAACT CATGTGCTCA GGACCCAGCA TCCTTTCTGT CTTCACCTCA GCCCTGCTCT 
TTTTCCAACC AACCACTGGC AGGTGCTGAC TCTGTTCCGA AACCCGTGTC TTTGTCATAT 
GTTGTCAGTC AAGAGCCAGG TGTTTTACAA CAGAAAAGTG CAGCTCAGAT GGCCAGTTCT 
GTTTTAGATA GTGATAATAA CTTTCCAGAG GATGTGGAAA ATACATTTGT CTTAGCAGAC 
ATTCAGAAGA CAAATTCTTT TTTACCAGCT TACTCTGAAA GCACTGTGCA AGAAGCACTC 
CCAAGCTTTG AGAAGGCCAA TACTTTGCTT GTGTTGCCAT CAGAAAAGGA CTTCAATGGG 
AGTGATACTG CTCAGCCAAA CACACATTAT GCATTTAGCA AACTCACATA CAAGTCTTCC 
AATAGCCAGG AAATTGAGAA GGGCCCAGAT GATACTCAAA TCATTTCACA TGAAAAGGAA 
AACAAATTAC AAAGTTTGGT CTTAACTCAG TTGAGTAAAT GTGATTCTGA TTTGTATGAG 
ATGAATGCAG GGCTGCCAAA AGGAAACCTG AATGAACAGG ATAACCCAAA GCATGGTCTT 
GACAGCGAAA AGTACTCGCT TTCCACTGAA GATGAAGAAT CTCAGCAGAG CACCTTATCA 
AGTGTGGAAA ACCATTCACA GCAGTCGGCC CAACCAGAGA TGCATAAATA TGACCAGCTT 
AGTAAAGTAG AACTAGAAGA AAATGCCGAA GACGATAAAA CTGAAAACCA GACCCCTCAA 
AGAGTGACTA GAAACAAAGC AAGCACAGTG GTCAATCCAA GCAAGCCCAT GCTTGCTGGC 
TGTGCCCTTG TAGCAGAGAA AGATAACGAG TCCTCCCCTA GGGGACGAAT CAGACTCACT 
GAAGAGGATG ATCCTCAGAT CCACCATCCT CGGAAGAGGA AAGTGTCACG GGTTCCTCAG 
CCTGTGCAAG TGAGCCCCTC TTTACTGCAA GCAAAGGAGA AAACTCAGCA GTCTCTAGCA 
GCCATTGTAG ATTCTCTGAA GTTAGATGAG ATTGAGCCAT ACAGTTCTGA GAGAGCAAAT 
CCATATTTTG AATATTTGCA CATAAGAAAA AAAATTGAAG AAAAACGCAA GTTGCTATGT 
AGCGTTATCC CTCAAGCTCC TCAGTATTAT GATGAATACG TAACGTTTAA TGGATCCTAT 
CTCCTGGATG GAAACCCCTT AAGCAAGATT TGTATTCCTA CAAGAAGGGA GAGAATCTCG 
GTTGATTTTC TCAGCTCATC AACCTTAAGG TCACCTTCAC TGCCATGCTT GTGCCAAAGG 
ATCACACCAC CACCTTCGCT GTCAGATCCA CTTAAAGAGC TCTTTCGGCA ACAGGAAGTA 
GTAAGGATGA AACTGCGTTT GCAACATAGT ATTGAAAGGG AAAAACTTAT TGTATCCAAT 
GAACAAGAAG TTCTGCGAGT TCATTACAGA GCTGCAAGAA CACTGGCCAA TCAAACACTG 
CCATTTAGTG CTTGTACTGT CTTACTGGAT GCAGAAGTGT ACAATGTGCC CTTGGACTCA 
CAGTCTGATG ACAGCAAAAC TTCTGTGAGA GATCGATTTA ATGCAAGACA GTTCATGTCC 
TGGTTGCAAG ATGTGGACGA TAAATTTGAC AAGCTAAAGA CCTGTCTCTT AATGAGGCAG 
CAACACGAAG CTGCAGCTTT AAATGCCGTC CAGAGGCTAG AATGGCAGCT CAAACTCCAG 
GAGCTGGACC CTGCCACGTA TAAGTCTCTC AGTATTTATG AAATCCAGGA GTTTTATGTT 
CCCCTTGTTG ATGTTAATGA TGATTTTGAA CTGACTCCTA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105280.1
CDS	1202..6526
Translation	MVNVPELLLDQSASRNDAKIINSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTRRERISVDFLSSSTLRSPSLPCLCQRITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSLSIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_030249420.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249420.1

ORF Insert Sequence:

ATGGTGAATG TGCCTGAATT ATTGCTTGAT CAAAGTGCGT CAAGGAATGA TGCAAAAATT 
ATCAATTCAG AAGAGGCTCA GTCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCA 
GAGACAGAGA AAGACTCTTT CGTCTGTGGC AGTAAAATCC TCCCAAGCAA AGCCCCGCTG 
CCTTCTGCAC TGGATGAGTA TGAGTTCAAA GATGATGATG AAGAAGAGAT AAGTAAGATG 
ATTGACGACA GGCACATTCT TAGGAAAGAA CAGCGGAGAG AGAATGAATC TGAAGCAGAA 
AGGAGTGGTC TGTTTGCAAA ACAGGAGAAA ACCTTCTATT CTAAGTCATT TAAAACAAAA 
AAACAGAAGC CGTCAAGGGT TCTGTGCTCC AGTACTGAAA GCTCTGATGA AGAGGGTCTT 
CAGAACAGAA AGATTTCTAC TGCTTGTTCT GTTGCTGAAT CATCACATCC TGATATGCAA 
GCCAAAAAAG AATATGAATA CAAACAGAAA GGCAAAGTTA AAAGAAAATT AAAAAATCAG 
AATAAAAACA AAGAGAACCA AGAGCTGAAA CAAGAAAAAG AGGGGAAAGA AAATAGCAGG 
ATAGCAACCT TGACAGTTAA TACTGCGCTG GATTGTTCAG AAAAATACAG AGAGGAGGGG 
AACTTCAGGA AATCTTTTAG CCCAAAGGAT GATACTTCAT TACACTTATT TCATATTTCC 
ACTGGTAAAT CTCCCAAACA TTCCTGTGGA TTAAGTGAAA AACAGACACC ACTGAAGCAA 
GAACACACGA AAGCATGCAT ATCCCCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTC 
ACTCGGTATG ATAATGCAGA ACCTGAAAGT TCAAATGTCA AATCTTATAA GCACAAGGAA 
AAAAACAAAC AGCAGAAAGA TTTTCACATA GAGTTTGGTG AAAAATCAAA TGCCAAAATT 
AAAGATGAAG ATCATAATCC AGCATTTGAA AACTCAGACT GCACACTGAA GAAGATGGAT 
AAAGAGGGTA AAACATTAAA AAAACATAAA TTAAAACATA AAGAGAAGGA TAAAGAAAAG 
CACAAAAAGG AAGTTGAAGG TGAAAAGGAA AAATATAAAA ATAAAGACAA TGCTAAAGAG 
CTACAGAGGA GCATCGAATT TGACAGAGAG TTTTGGAAAG AAAATTTTTT TAAAAGTGAT 
GAAACTGAGG ATCTCTTTTT AACTATGGAA CATGAGTCCA TAATAGAAAA AAAGACAAAA 
ATGGAAAAAA ACATGAAAGA TGATAGGTCA GCTAAAGAAA AGCATGTCTC AAAAGAAAGG 
AACTTTAAAG AAGAACGGGA AAAGACTAAA AAGGAAAATG AAAAATCTCT TAGGGAAGAA 
AAGGTAAAAG ATCCGAAAGA AGAAAAAGAC AGTGTATCCA CAGAGAAGGA GGCCGAGTGC 
TGTTCGGTGG GTGTCAGTGT GAGCCAGGAA GCTGCAGGGC TGCATTCATC AGAAAAGGAA 
ATGGATGCTG AGAAGCAAGA AAGACACACA AAAGAGAGGG AAAAGTCTGA TCGGCGCTTT 
CAGACTAAGG AGAAAGAGCT TGACAGGAAC GAGAGGAAAT TTTCTGAAAA AGAAAAGAAG 
ATAAAACATG AACATAAGTC AGAAAGAGAG AAGCTGGATT TCAGTGACTG CACTGACAGA 
GTGAGAGAGA AGGACAAGCT GTACTCACAC CAGACAGAAA GGTGCCACAA GGACAGCGAG 
AAGCTGAAAA ACACTGTCAG AAAGACTGAT GACAGAGAGA GGAACAGAGA AAAGGCGGAT 
AGAAAGCACG ACAGAGAGAA ACCTGAACGA GAGAGGTACT TAGCAGAAAA CAAAGAAAAG 
CACTTAGAGA AAAGAAAGCA ATCTGACAGC AGTGAGTATT CCAAATCAGA GAAGGTCAGA 
ACTAAAGACA GAGACAGGGA AGGAGAGAAG AAGGAGAAAC TTAGAGATAA AGAAAGTGTA 
AATGTAACTA ACTTGAAACA TTTGCAGGAA GAGAGAAAGT CCAGTACAGG AGACAGCAGT 
GGTAAAACCC AGCATGAGAA AGCTCTGTCC CTTAAAGAAA GGGCAAGAGA TGAGCCTTTG 
AAAACTCCAG ATGGAAAAGA GAAAGATAAA AAGGATATAG ACAGATACAA AGAGCGAGAC 
AAGCGTAAAG ATAAAGCCCA GCTAAATACT TTAATCAGAC TAAAATCTGA AACAGAGAAG 
CTTAAACCTA AATTATCACC AGCATCAAAA GATACTCGAC CTAAAGAAAA GAGGTTAGTA 
AATGATGATT TAATGCAGAC AAGTTTTGAA CGAATGCTTA GCCTTAAAGA CCTAGAAATA 
GAACAGTGGC ACAGAAAACA TAAGGAAAAA ATTAAGCAAA AGGAAAAAGA ACGATTACGC 
AATCGTACTT GTTTAGAACT TAAAGTAAAA GATAAAGAAA AAACAAAACA TGCACCCACA 
GAATCCAAAA GTAAAGAACT TACCAGGTCA AGAAGTTCAG AGTTGACTGA TGTTTATAGC 
AAGGAGAAAC AATCTAAAGA TGTTGGCAGT AATCGCTCAC AATCTGTTGA CAACAGAAAT 
GTATTGAATT TAGGAAAGCC ATCCTACATT TCAGAGAGTG GCTCCAACCG GTCTCCTAGG 
TCGGAAGGTG AGAGGCTGGG TCTTAGCTCT AGGTCTGTGT CCATGCTTTC TGTTGCTAGT 
TCAGAAGACT CTTGCCATAC AGTGACAACC CCAAGGCCTC TCGTTGAGTA TGACTCTGAC 
TTTATGTTAG AGGGCTCGGA ATCTCAGTTG TCATTTTCTC AGTCACCTTT TTTGCCAAAT 
GCCAAATCTC CAGCTCTTTA TGAAAGAGAG CTGGAGGGTC TCACTGAACT ACCAGACCGA 
CTTAAACCAC CATATACAAA CAGACTTCCA GTGTGCCATC TCAGGTCATC TTCTGTGGAA 
GATGTTAAAC TAATTACTAA TGAGGTAAGA CCTGCTGTAG AAATTAGAAG ATGCAGTATG 
CCCTCAGTTA TCTGTGAGCC CATAAAACAT TTCCAAGTAT CAGAAGAAAG CAGTCAAGGC 
AACTTAGCTG TTCCCAGAGA GACCTGTCTG TCTCCCAAGC CTGAAGTGTC TTCAGCAGTA 
CCTGAGAGGG GCCTTTCGCA TGTGTCTGAC TTACATCCTA ACTGTACAGG CTCTCCAACG 
AGGTCTGCAA ACAGCAGCTA TATTTCATCT GATGTAAATG TAATCAAGAG TGCAACTCTT 
GTGGGTACAC TGATTGACAG TTCGATGCAT TTGGAACCGT CTAATCAGGT TGGAGTGATT 
CCAAGTAAAT CATGGGAGAC GCCTGTTGAT AAACTGGAGT CTTTAAGCAC CAATGACTTC 
ATCTGTCCAG ATTCTAATAT ATCTGATCAA GATTCCTCTG CTCAGAGTTT TTATAATTCA 
GAAAACAAAA TGTTAAGAGA ACAAAATACT GACTGCTTAC CTCCGCACCA GCCTGAACTG 
CCAGGAAACT CATGTGCTCA GGACCCAGCA TCCTTTCTGT CTTCACCTCA GCCCTGCTCT 
TTTTCCAACC AACCACTGGC AGGTGCTGAC TCTGTTCCGA AACCCGTGTC TTTGTCATAT 
GTTGTCAGTC AAGAGCCAGG TGTTTTACAA CAGAAAAGTG CAGCTCAGAT GGCCAGTTCT 
GTTTTAGATA GTGATAATAA CTTTCCAGAG GATGTGGAAA ATACATTTGT CTTAGCAGAC 
ATTCAGAAGA CAAATTCTTT TTTACCAGCT TACTCTGAAA GCACTGTGCA AGAAGCACTC 
CCAAGCTTTG AGAAGGCCAA TACTTTGCTT GTGTTGCCAT CAGAAAAGGA CTTCAATGGG 
AGTGATACTG CTCAGCCAAA CACACATTAT GCATTTAGCA AACTCACATA CAAGTCTTCC 
AATAGCCAGG AAATTGAGAA GGGCCCAGAT GATACTCAAA TCATTTCACA TGAAAAGGAA 
AACAAATTAC AAAGTTTGGT CTTAACTCAG TTGAGTAAAT GTGATTCTGA TTTGTATGAG 
ATGAATGCAG GGCTGCCAAA AGGAAACCTG AATGAACAGG ATAACCCAAA GCATGGTCTT 
GACAGCGAAA AGTACTCGCT TTCCACTGAA GATGAAGAAT CTCAGCAGAG CACCTTATCA 
AGTGTGGAAA ACCATTCACA GCAGTCGGCC CAACCAGAGA TGCATAAATA TGACCAGCTT 
AGTAAAGTAG AACTAGAAGA AAATGCCGAA GACGATAAAA CTGAAAACCA GACCCCTCAA 
AGAGTGACTA GAAACAAAGC AAGCACAGTG GTCAATCCAA GCAAGCCCAT GCTTGCTGGC 
TGTGCCCTTG TAGCAGAGAA AGATAACGAG TCCTCCCCTA GGGGACGAAT CAGACTCACT 
GAAGAGGATG ATCCTCAGAT CCACCATCCT CGGAAGAGGA AAGTGTCACG GGTTCCTCAG 
CCTGTGCAAG TGAGCCCCTC TTTACTGCAA GCAAAGGAGA AAACTCAGCA GTCTCTAGCA 
GCCATTGTAG ATTCTCTGAA GTTAGATGAG ATTGAGCCAT ACAGTTCTGA GAGAGCAAAT 
CCATATTTTG AATATTTGCA CATAAGAAAA AAAATTGAAG AAAAACGCAA GTTGCTATGT 
AGCGTTATCC CTCAAGCTCC TCAGTATTAT GATGAATACG TAACGTTTAA TGGATCCTAT 
CTCCTGGATG GAAACCCCTT AAGCAAGATT TGTATTCCTA CAAGAAGGGA GAGAATCTCG 
GTTGATTTTC TCAGCTCATC AACCTTAAGG TCACCTTCAC TGCCATGCTT GTGCCAAAGG 
ATCACACCAC CACCTTCGCT GTCAGATCCA CTTAAAGAGC TCTTTCGGCA ACAGGAAGTA 
GTAAGGATGA AACTGCGTTT GCAACATAGT ATTGAAAGGG AAAAACTTAT TGTATCCAAT 
GAACAAGAAG TTCTGCGAGT TCATTACAGA GCTGCAAGAA CACTGGCCAA TCAAACACTG 
CCATTTAGTG CTTGTACTGT CTTACTGGAT GCAGAAGTGT ACAATGTGCC CTTGGACTCA 
CAGTCTGATG ACAGCAAAAC TTCTGTGAGA GATCGATTTA ATGCAAGACA GTTCATGTCC 
TGGTTGCAAG ATGTGGACGA TAAATTTGAC AAGCTAAAGA CCTGTCTCTT AATGAGGCAG 
CAACACGAAG CTGCAGCTTT AAATGCCGTC CAGAGGCTAG AATGGCAGCT CAAACTCCAG 
GAGCTGGACC CTGCCACGTA TAAGTCTCTC AGTATTTATG AAATCCAGGA GTTTTATGTT 
CCCCTTGTTG ATGTTAATGA TGATTTTGAA CTGACTCCTA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( XM_011246238.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244540.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X7, mRNA.

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CloneID	OMu72156
Clone ID Related Accession (Same CDS sequence)	XM_011246238.2 , XM_030249420.1
Accession Version	XM_011246238.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246238.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG TGCCTGAATT ATTGCTTGAT CAAAGTGCGT CAAGGAATGA TGCAAAAATT 
ATCAATTCAG AAGAGGCTCA GTCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCA 
GAGACAGAGA AAGACTCTTT CGTCTGTGGC AGTAAAATCC TCCCAAGCAA AGCCCCGCTG 
CCTTCTGCAC TGGATGAGTA TGAGTTCAAA GATGATGATG AAGAAGAGAT AAGTAAGATG 
ATTGACGACA GGCACATTCT TAGGAAAGAA CAGCGGAGAG AGAATGAATC TGAAGCAGAA 
AGGAGTGGTC TGTTTGCAAA ACAGGAGAAA ACCTTCTATT CTAAGTCATT TAAAACAAAA 
AAACAGAAGC CGTCAAGGGT TCTGTGCTCC AGTACTGAAA GCTCTGATGA AGAGGGTCTT 
CAGAACAGAA AGATTTCTAC TGCTTGTTCT GTTGCTGAAT CATCACATCC TGATATGCAA 
GCCAAAAAAG AATATGAATA CAAACAGAAA GGCAAAGTTA AAAGAAAATT AAAAAATCAG 
AATAAAAACA AAGAGAACCA AGAGCTGAAA CAAGAAAAAG AGGGGAAAGA AAATAGCAGG 
ATAGCAACCT TGACAGTTAA TACTGCGCTG GATTGTTCAG AAAAATACAG AGAGGAGGGG 
AACTTCAGGA AATCTTTTAG CCCAAAGGAT GATACTTCAT TACACTTATT TCATATTTCC 
ACTGGTAAAT CTCCCAAACA TTCCTGTGGA TTAAGTGAAA AACAGACACC ACTGAAGCAA 
GAACACACGA AAGCATGCAT ATCCCCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTC 
ACTCGGTATG ATAATGCAGA ACCTGAAAGT TCAAATGTCA AATCTTATAA GCACAAGGAA 
AAAAACAAAC AGCAGAAAGA TTTTCACATA GAGTTTGGTG AAAAATCAAA TGCCAAAATT 
AAAGATGAAG ATCATAATCC AGCATTTGAA AACTCAGACT GCACACTGAA GAAGATGGAT 
AAAGAGGGTA AAACATTAAA AAAACATAAA TTAAAACATA AAGAGAAGGA TAAAGAAAAG 
CACAAAAAGG AAGTTGAAGG TGAAAAGGAA AAATATAAAA ATAAAGACAA TGCTAAAGAG 
CTACAGAGGA GCATCGAATT TGACAGAGAG TTTTGGAAAG AAAATTTTTT TAAAAGTGAT 
GAAACTGAGG ATCTCTTTTT AACTATGGAA CATGAGTCCA TAATAGAAAA AAAGACAAAA 
ATGGAAAAAA ACATGAAAGA TGATAGGTCA GCTAAAGAAA AGCATGTCTC AAAAGAAAGG 
AACTTTAAAG AAGAACGGGA AAAGACTAAA AAGGAAAATG AAAAATCTCT TAGGGAAGAA 
AAGGTAAAAG ATCCGAAAGA AGAAAAAGAC AGTGTATCCA CAGAGAAGGA GGCCGAGTGC 
TGTTCGGTGG GTGTCAGTGT GAGCCAGGAA GCTGCAGGGC TGCATTCATC AGAAAAGGAA 
ATGGATGCTG AGAAGCAAGA AAGACACACA AAAGAGAGGG AAAAGTCTGA TCGGCGCTTT 
CAGACTAAGG AGAAAGAGCT TGACAGGAAC GAGAGGAAAT TTTCTGAAAA AGAAAAGAAG 
ATAAAACATG AACATAAGTC AGAAAGAGAG AAGCTGGATT TCAGTGACTG CACTGACAGA 
GTGAGAGAGA AGGACAAGCT GTACTCACAC CAGACAGAAA GGTGCCACAA GGACAGCGAG 
AAGCTGAAAA ACACTGTCAG AAAGACTGAT GACAGAGAGA GGAACAGAGA AAAGGCGGAT 
AGAAAGCACG ACAGAGAGAA ACCTGAACGA GAGAGGTACT TAGCAGAAAA CAAAGAAAAG 
CACTTAGAGA AAAGAAAGCA ATCTGACAGC AGTGAGTATT CCAAATCAGA GAAGGTCAGA 
ACTAAAGACA GAGACAGGGA AGGAGAGAAG AAGGAGAAAC TTAGAGATAA AGAAAGTGTA 
AATGTAACTA ACTTGAAACA TTTGCAGGAA GAGAGAAAGT CCAGTACAGG AGACAGCAGT 
GGTAAAACCC AGCATGAGAA AGCTCTGTCC CTTAAAGAAA GGGCAAGAGA TGAGCCTTTG 
AAAACTCCAG ATGGAAAAGA GAAAGATAAA AAGGATATAG ACAGATACAA AGAGCGAGAC 
AAGCGTAAAG ATAAAGCCCA GCTAAATACT TTAATCAGAC TAAAATCTGA AACAGAGAAG 
CTTAAACCTA AATTATCACC AGCATCAAAA GATACTCGAC CTAAAGAAAA GAGGTTAGTA 
AATGATGATT TAATGCAGAC AAGTTTTGAA CGAATGCTTA GCCTTAAAGA CCTAGAAATA 
GAACAGTGGC ACAGAAAACA TAAGGAAAAA ATTAAGCAAA AGGAAAAAGA ACGATTACGC 
AATCGTACTT GTTTAGAACT TAAAGTAAAA GATAAAGAAA AAACAAAACA TGCACCCACA 
GAATCCAAAA GTAAAGAACT TACCAGGTCA AGAAGTTCAG AGTTGACTGA TGTTTATAGC 
AAGGAGAAAC AATCTAAAGA TGTTGGCAGT AATCGCTCAC AATCTGTTGA CAACAGAAAT 
GTATTGAATT TAGGAAAGCC ATCCTACATT TCAGAGAGTG GCTCCAACCG GTCTCCTAGG 
TCGGAAGGTG AGAGGCTGGG TCTTAGCTCT AGGTCTGTGT CCATGCTTTC TGTTGCTAGT 
TCAGAAGACT CTTGCCATAC AGTGACAACC CCAAGGCCTC TCGTTGAGTA TGACTCTGAC 
TTTATGTTAG AGGGCTCGGA ATCTCAGTTG TCATTTTCTC AGTCACCTTT TTTGCCAAAT 
GCCAAATCTC CAGCTCTTTA TGAAAGAGAG CTGGAGGGTC TCACTGAACT ACCAGACCGA 
CTTAAACCAC CATATACAAA CAGACTTCCA GTGTGCCATC TCAGGTCATC TTCTGTGGAA 
GATGTTAAAC TAATTACTAA TGAGGTAAGA CCTGCTGTAG AAATTAGAAG ATGCAGTATG 
CCCTCAGTTA TCTGTGAGCC CATAAAACAT TTCCAAGTAT CAGAAGAAAG CAGTCAAGGC 
AACTTAGCTG TTCCCAGAGA GACCTGTCTG TCTCCCAAGC CTGAAGTGTC TTCAGCAGTA 
CCTGAGAGGG GCCTTTCGCA TGTGTCTGAC TTACATCCTA ACTGTACAGG CTCTCCAACG 
AGGTCTGCAA ACAGCAGCTA TATTTCATCT GATGTAAATG TAATCAAGAG TGCAACTCTT 
GTGGGTACAC TGATTGACAG TTCGATGCAT TTGGAACCGT CTAATCAGGT TGGAGTGATT 
CCAAGTAAAT CATGGGAGAC GCCTGTTGAT AAACTGGAGT CTTTAAGCAC CAATGACTTC 
ATCTGTCCAG ATTCTAATAT ATCTGATCAA GATTCCTCTG CTCAGAGTTT TTATAATTCA 
GAAAACAAAA TGTTAAGAGA ACAAAATACT GACTGCTTAC CTCCGCACCA GCCTGAACTG 
CCAGGAAACT CATGTGCTCA GGACCCAGCA TCCTTTCTGT CTTCACCTCA GCCCTGCTCT 
TTTTCCAACC AACCACTGGC AGGTGCTGAC TCTGTTCCGA AACCCGTGTC TTTGTCATAT 
GTTGTCAGTC AAGAGCCAGG TGTTTTACAA CAGAAAAGTG CAGCTCAGAT GGCCAGTTCT 
GTTTTAGATA GTGATAATAA CTTTCCAGAG GATGTGGAAA ATACATTTGT CTTAGCAGAC 
ATTCAGAAGA CAAATTCTTT TTTACCAGCT TACTCTGAAA GCACTGTGCA AGAAGCACTC 
CCAAGCTTTG AGAAGGCCAA TACTTTGCTT GTGTTGCCAT CAGAAAAGGA CTTCAATGGG 
AGTGATACTG CTCAGCCAAA CACACATTAT GCATTTAGCA AACTCACATA CAAGTCTTCC 
AATAGCCAGG AAATTGAGAA GGGCCCAGAT GATACTCAAA TCATTTCACA TGAAAAGGAA 
AACAAATTAC AAAGTTTGGT CTTAACTCAG TTGAGTAAAT GTGATTCTGA TTTGTATGAG 
ATGAATGCAG GGCTGCCAAA AGGAAACCTG AATGAACAGG ATAACCCAAA GCATGGTCTT 
GACAGCGAAA AGTACTCGCT TTCCACTGAA GATGAAGAAT CTCAGCAGAG CACCTTATCA 
AGTGTGGAAA ACCATTCACA GCAGTCGGCC CAACCAGAGA TGCATAAATA TGACCAGCTT 
AGTAAAGTAG AACTAGAAGA AAATGCCGAA GACGATAAAA CTGAAAACCA GACCCCTCAA 
AGAGTGACTA GAAACAAAGC AAGCACAGTG GTCAATCCAA GCAAGCCCAT GCTTGCTGGC 
TGTGCCCTTG TAGCAGAGAA AGATAACGAG TCCTCCCCTA GGGGACGAAT CAGACTCACT 
GAAGAGGATG ATCCTCAGAT CCACCATCCT CGGAAGAGGA AAGTGTCACG GGTTCCTCAG 
CCTGTGCAAG TGAGCCCCTC TTTACTGCAA GCAAAGGAGA AAACTCAGCA GTCTCTAGCA 
GCCATTGTAG ATTCTCTGAA GTTAGATGAG ATTGAGCCAT ACAGTTCTGA GAGAGCAAAT 
CCATATTTTG AATATTTGCA CATAAGAAAA AAAATTGAAG AAAAACGCAA GTTGCTATGT 
AGCGTTATCC CTCAAGCTCC TCAGTATTAT GATGAATACG TAACGTTTAA TGGATCCTAT 
CTCCTGGATG GAAACCCCTT AAGCAAGATT TGTATTCCTA CAAGAAGGGA GAGAATCTCG 
GTTGATTTTC TCAGCTCATC AACCTTAAGG TCACCTTCAC TGCCATGCTT GTGCCAAAGG 
ATCACACCAC CACCTTCGCT GTCAGATCCA CTTAAAGAGC TCTTTCGGCA ACAGGAAGTA 
GTAAGGATGA AACTGCGTTT GCAACATAGT ATTGAAAGGG AAAAACTTAT TGTATCCAAT 
GAACAAGAAG TTCTGCGAGT TCATTACAGA GCTGCAAGAA CACTGGCCAA TCAAACACTG 
CCATTTAGTG CTTGTACTGT CTTACTGGAT GCAGAAGTGT ACAATGTGCC CTTGGACTCA 
CAGTCTGATG ACAGCAAAAC TTCTGTGAGA GATCGATTTA ATGCAAGACA GTTCATGTCC 
TGGTTGCAAG ATGTGGACGA TAAATTTGAC AAGCTAAAGA CCTGTCTCTT AATGAGGCAG 
CAACACGAAG CTGCAGCTTT AAATGCCGTC CAGAGGCTAG AATGGCAGCT CAAACTCCAG 
GAGCTGGACC CTGCCACGTA TAAGTCTCTC AGTATTTATG AAATCCAGGA GTTTTATGTT 
CCCCTTGTTG ATGTTAATGA TGATTTTGAA CTGACTCCTA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244540.1
CDS	489..5813
Translation	MVNVPELLLDQSASRNDAKIINSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTRRERISVDFLSSSTLRSPSLPCLCQRITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSLSIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_011246238.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246238.2

ORF Insert Sequence:

ATGGTGAATG TGCCTGAATT ATTGCTTGAT CAAAGTGCGT CAAGGAATGA TGCAAAAATT 
ATCAATTCAG AAGAGGCTCA GTCTGTAAAT CCTTCTAGTG TTGATGAAAA TATTGACTCA 
GAGACAGAGA AAGACTCTTT CGTCTGTGGC AGTAAAATCC TCCCAAGCAA AGCCCCGCTG 
CCTTCTGCAC TGGATGAGTA TGAGTTCAAA GATGATGATG AAGAAGAGAT AAGTAAGATG 
ATTGACGACA GGCACATTCT TAGGAAAGAA CAGCGGAGAG AGAATGAATC TGAAGCAGAA 
AGGAGTGGTC TGTTTGCAAA ACAGGAGAAA ACCTTCTATT CTAAGTCATT TAAAACAAAA 
AAACAGAAGC CGTCAAGGGT TCTGTGCTCC AGTACTGAAA GCTCTGATGA AGAGGGTCTT 
CAGAACAGAA AGATTTCTAC TGCTTGTTCT GTTGCTGAAT CATCACATCC TGATATGCAA 
GCCAAAAAAG AATATGAATA CAAACAGAAA GGCAAAGTTA AAAGAAAATT AAAAAATCAG 
AATAAAAACA AAGAGAACCA AGAGCTGAAA CAAGAAAAAG AGGGGAAAGA AAATAGCAGG 
ATAGCAACCT TGACAGTTAA TACTGCGCTG GATTGTTCAG AAAAATACAG AGAGGAGGGG 
AACTTCAGGA AATCTTTTAG CCCAAAGGAT GATACTTCAT TACACTTATT TCATATTTCC 
ACTGGTAAAT CTCCCAAACA TTCCTGTGGA TTAAGTGAAA AACAGACACC ACTGAAGCAA 
GAACACACGA AAGCATGCAT ATCCCCAGGA AGTTCTGAAA TGTCATTACA GCCTGATCTC 
ACTCGGTATG ATAATGCAGA ACCTGAAAGT TCAAATGTCA AATCTTATAA GCACAAGGAA 
AAAAACAAAC AGCAGAAAGA TTTTCACATA GAGTTTGGTG AAAAATCAAA TGCCAAAATT 
AAAGATGAAG ATCATAATCC AGCATTTGAA AACTCAGACT GCACACTGAA GAAGATGGAT 
AAAGAGGGTA AAACATTAAA AAAACATAAA TTAAAACATA AAGAGAAGGA TAAAGAAAAG 
CACAAAAAGG AAGTTGAAGG TGAAAAGGAA AAATATAAAA ATAAAGACAA TGCTAAAGAG 
CTACAGAGGA GCATCGAATT TGACAGAGAG TTTTGGAAAG AAAATTTTTT TAAAAGTGAT 
GAAACTGAGG ATCTCTTTTT AACTATGGAA CATGAGTCCA TAATAGAAAA AAAGACAAAA 
ATGGAAAAAA ACATGAAAGA TGATAGGTCA GCTAAAGAAA AGCATGTCTC AAAAGAAAGG 
AACTTTAAAG AAGAACGGGA AAAGACTAAA AAGGAAAATG AAAAATCTCT TAGGGAAGAA 
AAGGTAAAAG ATCCGAAAGA AGAAAAAGAC AGTGTATCCA CAGAGAAGGA GGCCGAGTGC 
TGTTCGGTGG GTGTCAGTGT GAGCCAGGAA GCTGCAGGGC TGCATTCATC AGAAAAGGAA 
ATGGATGCTG AGAAGCAAGA AAGACACACA AAAGAGAGGG AAAAGTCTGA TCGGCGCTTT 
CAGACTAAGG AGAAAGAGCT TGACAGGAAC GAGAGGAAAT TTTCTGAAAA AGAAAAGAAG 
ATAAAACATG AACATAAGTC AGAAAGAGAG AAGCTGGATT TCAGTGACTG CACTGACAGA 
GTGAGAGAGA AGGACAAGCT GTACTCACAC CAGACAGAAA GGTGCCACAA GGACAGCGAG 
AAGCTGAAAA ACACTGTCAG AAAGACTGAT GACAGAGAGA GGAACAGAGA AAAGGCGGAT 
AGAAAGCACG ACAGAGAGAA ACCTGAACGA GAGAGGTACT TAGCAGAAAA CAAAGAAAAG 
CACTTAGAGA AAAGAAAGCA ATCTGACAGC AGTGAGTATT CCAAATCAGA GAAGGTCAGA 
ACTAAAGACA GAGACAGGGA AGGAGAGAAG AAGGAGAAAC TTAGAGATAA AGAAAGTGTA 
AATGTAACTA ACTTGAAACA TTTGCAGGAA GAGAGAAAGT CCAGTACAGG AGACAGCAGT 
GGTAAAACCC AGCATGAGAA AGCTCTGTCC CTTAAAGAAA GGGCAAGAGA TGAGCCTTTG 
AAAACTCCAG ATGGAAAAGA GAAAGATAAA AAGGATATAG ACAGATACAA AGAGCGAGAC 
AAGCGTAAAG ATAAAGCCCA GCTAAATACT TTAATCAGAC TAAAATCTGA AACAGAGAAG 
CTTAAACCTA AATTATCACC AGCATCAAAA GATACTCGAC CTAAAGAAAA GAGGTTAGTA 
AATGATGATT TAATGCAGAC AAGTTTTGAA CGAATGCTTA GCCTTAAAGA CCTAGAAATA 
GAACAGTGGC ACAGAAAACA TAAGGAAAAA ATTAAGCAAA AGGAAAAAGA ACGATTACGC 
AATCGTACTT GTTTAGAACT TAAAGTAAAA GATAAAGAAA AAACAAAACA TGCACCCACA 
GAATCCAAAA GTAAAGAACT TACCAGGTCA AGAAGTTCAG AGTTGACTGA TGTTTATAGC 
AAGGAGAAAC AATCTAAAGA TGTTGGCAGT AATCGCTCAC AATCTGTTGA CAACAGAAAT 
GTATTGAATT TAGGAAAGCC ATCCTACATT TCAGAGAGTG GCTCCAACCG GTCTCCTAGG 
TCGGAAGGTG AGAGGCTGGG TCTTAGCTCT AGGTCTGTGT CCATGCTTTC TGTTGCTAGT 
TCAGAAGACT CTTGCCATAC AGTGACAACC CCAAGGCCTC TCGTTGAGTA TGACTCTGAC 
TTTATGTTAG AGGGCTCGGA ATCTCAGTTG TCATTTTCTC AGTCACCTTT TTTGCCAAAT 
GCCAAATCTC CAGCTCTTTA TGAAAGAGAG CTGGAGGGTC TCACTGAACT ACCAGACCGA 
CTTAAACCAC CATATACAAA CAGACTTCCA GTGTGCCATC TCAGGTCATC TTCTGTGGAA 
GATGTTAAAC TAATTACTAA TGAGGTAAGA CCTGCTGTAG AAATTAGAAG ATGCAGTATG 
CCCTCAGTTA TCTGTGAGCC CATAAAACAT TTCCAAGTAT CAGAAGAAAG CAGTCAAGGC 
AACTTAGCTG TTCCCAGAGA GACCTGTCTG TCTCCCAAGC CTGAAGTGTC TTCAGCAGTA 
CCTGAGAGGG GCCTTTCGCA TGTGTCTGAC TTACATCCTA ACTGTACAGG CTCTCCAACG 
AGGTCTGCAA ACAGCAGCTA TATTTCATCT GATGTAAATG TAATCAAGAG TGCAACTCTT 
GTGGGTACAC TGATTGACAG TTCGATGCAT TTGGAACCGT CTAATCAGGT TGGAGTGATT 
CCAAGTAAAT CATGGGAGAC GCCTGTTGAT AAACTGGAGT CTTTAAGCAC CAATGACTTC 
ATCTGTCCAG ATTCTAATAT ATCTGATCAA GATTCCTCTG CTCAGAGTTT TTATAATTCA 
GAAAACAAAA TGTTAAGAGA ACAAAATACT GACTGCTTAC CTCCGCACCA GCCTGAACTG 
CCAGGAAACT CATGTGCTCA GGACCCAGCA TCCTTTCTGT CTTCACCTCA GCCCTGCTCT 
TTTTCCAACC AACCACTGGC AGGTGCTGAC TCTGTTCCGA AACCCGTGTC TTTGTCATAT 
GTTGTCAGTC AAGAGCCAGG TGTTTTACAA CAGAAAAGTG CAGCTCAGAT GGCCAGTTCT 
GTTTTAGATA GTGATAATAA CTTTCCAGAG GATGTGGAAA ATACATTTGT CTTAGCAGAC 
ATTCAGAAGA CAAATTCTTT TTTACCAGCT TACTCTGAAA GCACTGTGCA AGAAGCACTC 
CCAAGCTTTG AGAAGGCCAA TACTTTGCTT GTGTTGCCAT CAGAAAAGGA CTTCAATGGG 
AGTGATACTG CTCAGCCAAA CACACATTAT GCATTTAGCA AACTCACATA CAAGTCTTCC 
AATAGCCAGG AAATTGAGAA GGGCCCAGAT GATACTCAAA TCATTTCACA TGAAAAGGAA 
AACAAATTAC AAAGTTTGGT CTTAACTCAG TTGAGTAAAT GTGATTCTGA TTTGTATGAG 
ATGAATGCAG GGCTGCCAAA AGGAAACCTG AATGAACAGG ATAACCCAAA GCATGGTCTT 
GACAGCGAAA AGTACTCGCT TTCCACTGAA GATGAAGAAT CTCAGCAGAG CACCTTATCA 
AGTGTGGAAA ACCATTCACA GCAGTCGGCC CAACCAGAGA TGCATAAATA TGACCAGCTT 
AGTAAAGTAG AACTAGAAGA AAATGCCGAA GACGATAAAA CTGAAAACCA GACCCCTCAA 
AGAGTGACTA GAAACAAAGC AAGCACAGTG GTCAATCCAA GCAAGCCCAT GCTTGCTGGC 
TGTGCCCTTG TAGCAGAGAA AGATAACGAG TCCTCCCCTA GGGGACGAAT CAGACTCACT 
GAAGAGGATG ATCCTCAGAT CCACCATCCT CGGAAGAGGA AAGTGTCACG GGTTCCTCAG 
CCTGTGCAAG TGAGCCCCTC TTTACTGCAA GCAAAGGAGA AAACTCAGCA GTCTCTAGCA 
GCCATTGTAG ATTCTCTGAA GTTAGATGAG ATTGAGCCAT ACAGTTCTGA GAGAGCAAAT 
CCATATTTTG AATATTTGCA CATAAGAAAA AAAATTGAAG AAAAACGCAA GTTGCTATGT 
AGCGTTATCC CTCAAGCTCC TCAGTATTAT GATGAATACG TAACGTTTAA TGGATCCTAT 
CTCCTGGATG GAAACCCCTT AAGCAAGATT TGTATTCCTA CAAGAAGGGA GAGAATCTCG 
GTTGATTTTC TCAGCTCATC AACCTTAAGG TCACCTTCAC TGCCATGCTT GTGCCAAAGG 
ATCACACCAC CACCTTCGCT GTCAGATCCA CTTAAAGAGC TCTTTCGGCA ACAGGAAGTA 
GTAAGGATGA AACTGCGTTT GCAACATAGT ATTGAAAGGG AAAAACTTAT TGTATCCAAT 
GAACAAGAAG TTCTGCGAGT TCATTACAGA GCTGCAAGAA CACTGGCCAA TCAAACACTG 
CCATTTAGTG CTTGTACTGT CTTACTGGAT GCAGAAGTGT ACAATGTGCC CTTGGACTCA 
CAGTCTGATG ACAGCAAAAC TTCTGTGAGA GATCGATTTA ATGCAAGACA GTTCATGTCC 
TGGTTGCAAG ATGTGGACGA TAAATTTGAC AAGCTAAAGA CCTGTCTCTT AATGAGGCAG 
CAACACGAAG CTGCAGCTTT AAATGCCGTC CAGAGGCTAG AATGGCAGCT CAAACTCCAG 
GAGCTGGACC CTGCCACGTA TAAGTCTCTC AGTATTTATG AAATCCAGGA GTTTTATGTT 
CCCCTTGTTG ATGTTAATGA TGATTTTGAA CTGACTCCTA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( XM_006523395.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523458.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X3, mRNA.

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CloneID	OMu52953
Clone ID Related Accession (Same CDS sequence)	XM_006523395.3 , XM_006523395.4 , XM_006523394.3 , XM_006523393.4 , XM_006523394.4 , XM_006523393.3
Accession Version	XM_006523395.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523395.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AAGAAGGGAG AGAATCTCGG TTGATTTTCT CAGCTCATCA 
ACCTTAAGGT CACCTTCACT GCCATGCTTG TGCCAAAGGA TCACACCACC ACCTTCGCTG 
TCAGATCCAC TTAAAGAGCT CTTTCGGCAA CAGGAAGTAG TAAGGATGAA ACTGCGTTTG 
CAACATAGTA TTGAAAGGGA AAAACTTATT GTATCCAATG AACAAGAAGT TCTGCGAGTT 
CATTACAGAG CTGCAAGAAC ACTGGCCAAT CAAACACTGC CATTTAGTGC TTGTACTGTC 
TTACTGGATG CAGAAGTGTA CAATGTGCCC TTGGACTCAC AGTCTGATGA CAGCAAAACT 
TCTGTGAGAG ATCGATTTAA TGCAAGACAG TTCATGTCCT GGTTGCAAGA TGTGGACGAT 
AAATTTGACA AGCTAAAGAC CTGTCTCTTA ATGAGGCAGC AACACGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGCTAGA ATGGCAGCTC AAACTCCAGG AGCTGGACCC TGCCACGTAT 
AAGTCTCTCA GTATTTATGA AATCCAGGAG TTTTATGTTC CCCTTGTTGA TGTTAATGAT 
GATTTTGAAC TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523458.1
CDS	357..6560
Translation	MPKSGFTKPVQSENSDSDSSMVERPYGRKSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSGDDESYTDSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTRRERISVDFLSSSTLRSPSLPCLCQRITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSLSIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_006523395.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523395.4

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AAGAAGGGAG AGAATCTCGG TTGATTTTCT CAGCTCATCA 
ACCTTAAGGT CACCTTCACT GCCATGCTTG TGCCAAAGGA TCACACCACC ACCTTCGCTG 
TCAGATCCAC TTAAAGAGCT CTTTCGGCAA CAGGAAGTAG TAAGGATGAA ACTGCGTTTG 
CAACATAGTA TTGAAAGGGA AAAACTTATT GTATCCAATG AACAAGAAGT TCTGCGAGTT 
CATTACAGAG CTGCAAGAAC ACTGGCCAAT CAAACACTGC CATTTAGTGC TTGTACTGTC 
TTACTGGATG CAGAAGTGTA CAATGTGCCC TTGGACTCAC AGTCTGATGA CAGCAAAACT 
TCTGTGAGAG ATCGATTTAA TGCAAGACAG TTCATGTCCT GGTTGCAAGA TGTGGACGAT 
AAATTTGACA AGCTAAAGAC CTGTCTCTTA ATGAGGCAGC AACACGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGCTAGA ATGGCAGCTC AAACTCCAGG AGCTGGACCC TGCCACGTAT 
AAGTCTCTCA GTATTTATGA AATCCAGGAG TTTTATGTTC CCCTTGTTGA TGTTAATGAT 
GATTTTGAAC TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( XM_006523393.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523456.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X1, mRNA.

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CloneID	OMu52953
Clone ID Related Accession (Same CDS sequence)	XM_006523395.3 , XM_006523395.4 , XM_006523394.3 , XM_006523393.4 , XM_006523394.4 , XM_006523393.3
Accession Version	XM_006523393.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523393.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AAGAAGGGAG AGAATCTCGG TTGATTTTCT CAGCTCATCA 
ACCTTAAGGT CACCTTCACT GCCATGCTTG TGCCAAAGGA TCACACCACC ACCTTCGCTG 
TCAGATCCAC TTAAAGAGCT CTTTCGGCAA CAGGAAGTAG TAAGGATGAA ACTGCGTTTG 
CAACATAGTA TTGAAAGGGA AAAACTTATT GTATCCAATG AACAAGAAGT TCTGCGAGTT 
CATTACAGAG CTGCAAGAAC ACTGGCCAAT CAAACACTGC CATTTAGTGC TTGTACTGTC 
TTACTGGATG CAGAAGTGTA CAATGTGCCC TTGGACTCAC AGTCTGATGA CAGCAAAACT 
TCTGTGAGAG ATCGATTTAA TGCAAGACAG TTCATGTCCT GGTTGCAAGA TGTGGACGAT 
AAATTTGACA AGCTAAAGAC CTGTCTCTTA ATGAGGCAGC AACACGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGCTAGA ATGGCAGCTC AAACTCCAGG AGCTGGACCC TGCCACGTAT 
AAGTCTCTCA GTATTTATGA AATCCAGGAG TTTTATGTTC CCCTTGTTGA TGTTAATGAT 
GATTTTGAAC TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523456.1
CDS	1973..8176
Translation	MPKSGFTKPVQSENSDSDSSMVERPYGRKSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSGDDESYTDSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTRRERISVDFLSSSTLRSPSLPCLCQRITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSLSIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_006523393.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523393.4

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AAGAAGGGAG AGAATCTCGG TTGATTTTCT CAGCTCATCA 
ACCTTAAGGT CACCTTCACT GCCATGCTTG TGCCAAAGGA TCACACCACC ACCTTCGCTG 
TCAGATCCAC TTAAAGAGCT CTTTCGGCAA CAGGAAGTAG TAAGGATGAA ACTGCGTTTG 
CAACATAGTA TTGAAAGGGA AAAACTTATT GTATCCAATG AACAAGAAGT TCTGCGAGTT 
CATTACAGAG CTGCAAGAAC ACTGGCCAAT CAAACACTGC CATTTAGTGC TTGTACTGTC 
TTACTGGATG CAGAAGTGTA CAATGTGCCC TTGGACTCAC AGTCTGATGA CAGCAAAACT 
TCTGTGAGAG ATCGATTTAA TGCAAGACAG TTCATGTCCT GGTTGCAAGA TGTGGACGAT 
AAATTTGACA AGCTAAAGAC CTGTCTCTTA ATGAGGCAGC AACACGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGCTAGA ATGGCAGCTC AAACTCCAGG AGCTGGACCC TGCCACGTAT 
AAGTCTCTCA GTATTTATGA AATCCAGGAG TTTTATGTTC CCCTTGTTGA TGTTAATGAT 
GATTTTGAAC TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( XM_006523394.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523457.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X2, mRNA.

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CloneID	OMu52953
Clone ID Related Accession (Same CDS sequence)	XM_006523395.3 , XM_006523395.4 , XM_006523394.3 , XM_006523393.4 , XM_006523394.4 , XM_006523393.3
Accession Version	XM_006523394.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523394.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AAGAAGGGAG AGAATCTCGG TTGATTTTCT CAGCTCATCA 
ACCTTAAGGT CACCTTCACT GCCATGCTTG TGCCAAAGGA TCACACCACC ACCTTCGCTG 
TCAGATCCAC TTAAAGAGCT CTTTCGGCAA CAGGAAGTAG TAAGGATGAA ACTGCGTTTG 
CAACATAGTA TTGAAAGGGA AAAACTTATT GTATCCAATG AACAAGAAGT TCTGCGAGTT 
CATTACAGAG CTGCAAGAAC ACTGGCCAAT CAAACACTGC CATTTAGTGC TTGTACTGTC 
TTACTGGATG CAGAAGTGTA CAATGTGCCC TTGGACTCAC AGTCTGATGA CAGCAAAACT 
TCTGTGAGAG ATCGATTTAA TGCAAGACAG TTCATGTCCT GGTTGCAAGA TGTGGACGAT 
AAATTTGACA AGCTAAAGAC CTGTCTCTTA ATGAGGCAGC AACACGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGCTAGA ATGGCAGCTC AAACTCCAGG AGCTGGACCC TGCCACGTAT 
AAGTCTCTCA GTATTTATGA AATCCAGGAG TTTTATGTTC CCCTTGTTGA TGTTAATGAT 
GATTTTGAAC TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523457.1
CDS	538..6741
Translation	MPKSGFTKPVQSENSDSDSSMVERPYGRKSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSGDDESYTDSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTRRERISVDFLSSSTLRSPSLPCLCQRITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSLSIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_006523394.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523394.4

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AAGAAGGGAG AGAATCTCGG TTGATTTTCT CAGCTCATCA 
ACCTTAAGGT CACCTTCACT GCCATGCTTG TGCCAAAGGA TCACACCACC ACCTTCGCTG 
TCAGATCCAC TTAAAGAGCT CTTTCGGCAA CAGGAAGTAG TAAGGATGAA ACTGCGTTTG 
CAACATAGTA TTGAAAGGGA AAAACTTATT GTATCCAATG AACAAGAAGT TCTGCGAGTT 
CATTACAGAG CTGCAAGAAC ACTGGCCAAT CAAACACTGC CATTTAGTGC TTGTACTGTC 
TTACTGGATG CAGAAGTGTA CAATGTGCCC TTGGACTCAC AGTCTGATGA CAGCAAAACT 
TCTGTGAGAG ATCGATTTAA TGCAAGACAG TTCATGTCCT GGTTGCAAGA TGTGGACGAT 
AAATTTGACA AGCTAAAGAC CTGTCTCTTA ATGAGGCAGC AACACGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGCTAGA ATGGCAGCTC AAACTCCAGG AGCTGGACCC TGCCACGTAT 
AAGTCTCTCA GTATTTATGA AATCCAGGAG TTTTATGTTC CCCTTGTTGA TGTTAATGAT 
GATTTTGAAC TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( XM_006523396.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523459.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X4, mRNA.

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CloneID	OMu52954
Clone ID Related Accession (Same CDS sequence)	XM_006523396.3 , XM_006523396.4
Accession Version	XM_006523396.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523396.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AAGAAGGGAG AGAATCTCGG TTGATTTTCT CAGCTCATCA 
ACCTTAAGGT CACCTTCACT GCCATGCTTG TGCCAAAGGA TCACACCACC ACCTTCGCTG 
TCAGATCCAC TTAAAGAGCT CTTTCGGCAA CAGGAAGTAG TAAGGATGAA ACTGCGTTTG 
CAACATAGTA TTGAAAGGGA AAAACTTATT GTATCCAATG AACAAGAAGT TCTGCGAGTT 
CATTACAGAG CTGCAAGAAC ACTGGCCAAT CAAACACTGC CATTTAGTGC TTGTACTGTC 
TTACTGGATG CAGAAGTGTA CAATGTGCCC TTGGACTCAC AGTCTGATGA CAGCAAAACT 
TCTGTGAGAG ATCGATTTAA TGCAAGACAG TTCATGTCCT GGTTGCAAGA TGTGGACGAT 
AAATTTGACA AGCTAAAGAA AGAAAATTTG ATGAGCTCGT GTGGCAGATT ACAAAAATTA 
AAAAAGATTA AATGTTCAAG CAAGCAAGAG AAAAGGAAAT CTGTAGAGCA TTTGGTTCTG 
ATGGTCCCGC CAGGGACCAG TCGAGGAAGT AGTACTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523459.1
CDS	258..6419
Translation	MPKSGFTKPVQSENSDSDSSMVERPYGRKSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSGDDESYTDSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTRRERISVDFLSSSTLRSPSLPCLCQRITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKKENLMSSCGRLQKLKKIKCSSKQEKRKSVEHLVLMVPPGTSRGSSTD

Target ORF information:

RefSeq Version	XM_006523396.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523396.4

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AAGAAGGGAG AGAATCTCGG TTGATTTTCT CAGCTCATCA 
ACCTTAAGGT CACCTTCACT GCCATGCTTG TGCCAAAGGA TCACACCACC ACCTTCGCTG 
TCAGATCCAC TTAAAGAGCT CTTTCGGCAA CAGGAAGTAG TAAGGATGAA ACTGCGTTTG 
CAACATAGTA TTGAAAGGGA AAAACTTATT GTATCCAATG AACAAGAAGT TCTGCGAGTT 
CATTACAGAG CTGCAAGAAC ACTGGCCAAT CAAACACTGC CATTTAGTGC TTGTACTGTC 
TTACTGGATG CAGAAGTGTA CAATGTGCCC TTGGACTCAC AGTCTGATGA CAGCAAAACT 
TCTGTGAGAG ATCGATTTAA TGCAAGACAG TTCATGTCCT GGTTGCAAGA TGTGGACGAT 
AAATTTGACA AGCTAAAGAA AGAAAATTTG ATGAGCTCGT GTGGCAGATT ACAAAAATTA 
AAAAAGATTA AATGTTCAAG CAAGCAAGAG AAAAGGAAAT CTGTAGAGCA TTTGGTTCTG 
ATGGTCCCGC CAGGGACCAG TCGAGGAAGT AGTACTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( XM_030249418.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105278.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X7, mRNA.

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CloneID	OMu52955
Clone ID Related Accession (Same CDS sequence)	XM_030249418.1 , XM_006523397.4 , XM_030249417.1 , XM_006523397.3
Accession Version	XM_030249418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGAAAAA 
GAAGGTCCAG AGAAGAAGAA AACAAAAAAA GAAGCTGGAA CTAAGAAGTC CACCCCAGTT 
AGCATTCTTT TTGGTTATCC ACTTTCTGAA CGAAAGCAGA TGGCACTCCT AATGCAGATG 
ACAGCAAGGG ACAACAGCCC AGATTCCACA CCAAGTCATC CGTCACAGGC AACGCCAGCC 
CAGAAGAAAA CGCCCAGCTC TTCATCTCGA CAGAAAGATA AAATTAATAA AAGAAATGAG 
CGTGGCGAAA CTCCTTTACA CATGGCCGCT ATTCGAGGAG ACGTGAAGCA AGTCAAAGAG 
TTAATTAGTT TAGGGGCAAA CGTCAATGTG AAGGACTTTG CAGGTTGGAC CCCTTTGCAT 
GAAGCTTGCA ATGTTGGCTA TTACGATGTT GCTAAGATAC TGATAGCAGC TGGCGCAGAC 
GTTAACACAC AAGGACTGGA TGATGACACC CCGCTCCATG ACTCTGCCAG CAGTGGCCAT 
AGAGACATAG TGAAGTTGTT GCTTCGTCAT GGCGGGAATC CATTCCAAGC TAATAAACAT 
GGAGAACGGC CAGTGGATGT GGCAGAGACA GAGGAGTTGG AGTTGCTGCT AAAAAGAGAG 
GTGCCTTTGT CTGGCGATGA TGAAAGTTAT ACAGATTCAG AAGAGGCTCA GTCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCA GAGACAGAGA AAGACTCTTT CGTCTGTGGC 
AGTAAAATCC TCCCAAGCAA AGCCCCGCTG CCTTCTGCAC TGGATGAGTA TGAGTTCAAA 
GATGATGATG AAGAAGAGAT AAGTAAGATG ATTGACGACA GGCACATTCT TAGGAAAGAA 
CAGCGGAGAG AGAATGAATC TGAAGCAGAA AGGAGTGGTC TGTTTGCAAA ACAGGAGAAA 
ACCTTCTATT CTAAGTCATT TAAAACAAAA AAACAGAAGC CGTCAAGGGT TCTGTGCTCC 
AGTACTGAAA GCTCTGATGA AGAGGGTCTT CAGAACAGAA AGATTTCTAC TGCTTGTTCT 
GTTGCTGAAT CATCACATCC TGATATGCAA GCCAAAAAAG AATATGAATA CAAACAGAAA 
GGCAAAGTTA AAAGAAAATT AAAAAATCAG AATAAAAACA AAGAGAACCA AGAGCTGAAA 
CAAGAAAAAG AGGGGAAAGA AAATAGCAGG ATAGCAACCT TGACAGTTAA TACTGCGCTG 
GATTGTTCAG AAAAATACAG AGAGGAGGGG AACTTCAGGA AATCTTTTAG CCCAAAGGAT 
GATACTTCAT TACACTTATT TCATATTTCC ACTGGTAAAT CTCCCAAACA TTCCTGTGGA 
TTAAGTGAAA AACAGACACC ACTGAAGCAA GAACACACGA AAGCATGCAT ATCCCCAGGA 
AGTTCTGAAA TGTCATTACA GCCTGATCTC ACTCGGTATG ATAATGCAGA ACCTGAAAGT 
TCAAATGTCA AATCTTATAA GCACAAGGAA AAAAACAAAC AGCAGAAAGA TTTTCACATA 
GAGTTTGGTG AAAAATCAAA TGCCAAAATT AAAGATGAAG ATCATAATCC AGCATTTGAA 
AACTCAGACT GCACACTGAA GAAGATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAA 
TTAAAACATA AAGAGAAGGA TAAAGAAAAG CACAAAAAGG AAGTTGAAGG TGAAAAGGAA 
AAATATAAAA ATAAAGACAA TGCTAAAGAG CTACAGAGGA GCATCGAATT TGACAGAGAG 
TTTTGGAAAG AAAATTTTTT TAAAAGTGAT GAAACTGAGG ATCTCTTTTT AACTATGGAA 
CATGAGTCCA TAATAGAAAA AAAGACAAAA ATGGAAAAAA ACATGAAAGA TGATAGGTCA 
GCTAAAGAAA AGCATGTCTC AAAAGAAAGG AACTTTAAAG AAGAACGGGA AAAGACTAAA 
AAGGAAAATG AAAAATCTCT TAGGGAAGAA AAGGTAAAAG ATCCGAAAGA AGAAAAAGAC 
AGTGTATCCA CAGAGAAGGA GGCCGAGTGC TGTTCGGTGG GTGTCAGTGT GAGCCAGGAA 
GCTGCAGGGC TGCATTCATC AGAAAAGGAA ATGGATGCTG AGAAGCAAGA AAGACACACA 
AAAGAGAGGG AAAAGTCTGA TCGGCGCTTT CAGACTAAGG AGAAAGAGCT TGACAGGAAC 
GAGAGGAAAT TTTCTGAAAA AGAAAAGAAG ATAAAACATG AACATAAGTC AGAAAGAGAG 
AAGCTGGATT TCAGTGACTG CACTGACAGA GTGAGAGAGA AGGACAAGCT GTACTCACAC 
CAGACAGAAA GGTGCCACAA GGACAGCGAG AAGCTGAAAA ACACTGTCAG AAAGACTGAT 
GACAGAGAGA GGAACAGAGA AAAGGCGGAT AGAAAGCACG ACAGAGAGAA ACCTGAACGA 
GAGAGGTACT TAGCAGAAAA CAAAGAAAAG CACTTAGAGA AAAGAAAGCA ATCTGACAGC 
AGTGAGTATT CCAAATCAGA GAAGGTCAGA ACTAAAGACA GAGACAGGGA AGGAGAGAAG 
AAGGAGAAAC TTAGAGATAA AGAAAGTGTA AATGTAACTA ACTTGAAACA TTTGCAGGAA 
GAGAGAAAGT CCAGTACAGG AGACAGCAGT GGTAAAACCC AGCATGAGAA AGCTCTGTCC 
CTTAAAGAAA GGGCAAGAGA TGAGCCTTTG AAAACTCCAG ATGGAAAAGA GAAAGATAAA 
AAGGATATAG ACAGATACAA AGAGCGAGAC AAGCGTAAAG ATAAAGCCCA GCTAAATACT 
TTAATCAGAC TAAAATCTGA AACAGAGAAG CTTAAACCTA AATTATCACC AGCATCAAAA 
GATACTCGAC CTAAAGAAAA GAGGTTAGTA AATGATGATT TAATGCAGAC AAGTTTTGAA 
CGAATGCTTA GCCTTAAAGA CCTAGAAATA GAACAGTGGC ACAGAAAACA TAAGGAAAAA 
ATTAAGCAAA AGGAAAAAGA ACGATTACGC AATCGTACTT GTTTAGAACT TAAAGTAAAA 
GATAAAGAAA AAACAAAACA TGCACCCACA GAATCCAAAA GTAAAGAACT TACCAGGTCA 
AGAAGTTCAG AGTTGACTGA TGTTTATAGC AAGGAGAAAC AATCTAAAGA TGTTGGCAGT 
AATCGCTCAC AATCTGTTGA CAACAGAAAT GTATTGAATT TAGGAAAGCC ATCCTACATT 
TCAGAGAGTG GCTCCAACCG GTCTCCTAGG TCGGAAGGTG AGAGGCTGGG TCTTAGCTCT 
AGGTCTGTGT CCATGCTTTC TGTTGCTAGT TCAGAAGACT CTTGCCATAC AGTGACAACC 
CCAAGGCCTC TCGTTGAGTA TGACTCTGAC TTTATGTTAG AGGGCTCGGA ATCTCAGTTG 
TCATTTTCTC AGTCACCTTT TTTGCCAAAT GCCAAATCTC CAGCTCTTTA TGAAAGAGAG 
CTGGAGGGTC TCACTGAACT ACCAGACCGA CTTAAACCAC CATATACAAA CAGACTTCCA 
GTGTGCCATC TCAGGTCATC TTCTGTGGAA GATGTTAAAC TAATTACTAA TGAGGTAAGA 
CCTGCTGTAG AAATTAGAAG ATGCAGTATG CCCTCAGTTA TCTGTGAGCC CATAAAACAT 
TTCCAAGTAT CAGAAGAAAG CAGTCAAGGC AACTTAGCTG TTCCCAGAGA GACCTGTCTG 
TCTCCCAAGC CTGAAGTGTC TTCAGCAGTA CCTGAGAGGG GCCTTTCGCA TGTGTCTGAC 
TTACATCCTA ACTGTACAGG CTCTCCAACG AGGTCTGCAA ACAGCAGCTA TATTTCATCT 
GATGTAAATG TAATCAAGAG TGCAACTCTT GTGGGTACAC TGATTGACAG TTCGATGCAT 
TTGGAACCGT CTAATCAGGT TGGAGTGATT CCAAGTAAAT CATGGGAGAC GCCTGTTGAT 
AAACTGGAGT CTTTAAGCAC CAATGACTTC ATCTGTCCAG ATTCTAATAT ATCTGATCAA 
GATTCCTCTG CTCAGAGTTT TTATAATTCA GAAAACAAAA TGTTAAGAGA ACAAAATACT 
GACTGCTTAC CTCCGCACCA GCCTGAACTG CCAGGAAACT CATGTGCTCA GGACCCAGCA 
TCCTTTCTGT CTTCACCTCA GCCCTGCTCT TTTTCCAACC AACCACTGGC AGGTGCTGAC 
TCTGTTCCGA AACCCGTGTC TTTGTCATAT GTTGTCAGTC AAGAGCCAGG TGTTTTACAA 
CAGAAAAGTG CAGCTCAGAT GGCCAGTTCT GTTTTAGATA GTGATAATAA CTTTCCAGAG 
GATGTGGAAA ATACATTTGT CTTAGCAGAC ATTCAGAAGA CAAATTCTTT TTTACCAGCT 
TACTCTGAAA GCACTGTGCA AGAAGCACTC CCAAGCTTTG AGAAGGCCAA TACTTTGCTT 
GTGTTGCCAT CAGAAAAGGA CTTCAATGGG AGTGATACTG CTCAGCCAAA CACACATTAT 
GCATTTAGCA AACTCACATA CAAGTCTTCC AATAGCCAGG AAATTGAGAA GGGCCCAGAT 
GATACTCAAA TCATTTCACA TGAAAAGGAA AACAAATTAC AAAGTTTGGT CTTAACTCAG 
TTGAGTAAAT GTGATTCTGA TTTGTATGAG ATGAATGCAG GGCTGCCAAA AGGAAACCTG 
AATGAACAGG ATAACCCAAA GCATGGTCTT GACAGCGAAA AGTACTCGCT TTCCACTGAA 
GATGAAGAAT CTCAGCAGAG CACCTTATCA AGTGTGGAAA ACCATTCACA GCAGTCGGCC 
CAACCAGAGA TGCATAAATA TGACCAGCTT AGTAAAGTAG AACTAGAAGA AAATGCCGAA 
GACGATAAAA CTGAAAACCA GACCCCTCAA AGAGTGACTA GAAACAAAGC AAGCACAGTG 
GTCAATCCAA GCAAGCCCAT GCTTGCTGGC TGTGCCCTTG TAGCAGAGAA AGATAACGAG 
TCCTCCCCTA GGGGACGAAT CAGACTCACT GAAGAGGATG ATCCTCAGAT CCACCATCCT 
CGGAAGAGGA AAGTGTCACG GGTTCCTCAG CCTGTGCAAG TGAGCCCCTC TTTACTGCAA 
GCAAAGGAGA AAACTCAGCA GTCTCTAGCA GCCATTGTAG ATTCTCTGAA GTTAGATGAG 
ATTGAGCCAT ACAGTTCTGA GAGAGCAAAT CCATATTTTG AATATTTGCA CATAAGAAAA 
AAAATTGAAG AAAAACGCAA GTTGCTATGT AGCGTTATCC CTCAAGCTCC TCAGTATTAT 
GATGAATACG TAACGTTTAA TGGATCCTAT CTCCTGGATG GAAACCCCTT AAGCAAGATT 
TGTATTCCTA CAAGAAGGGA GAGAATCTCG GTTGATTTTC TCAGCTCATC AACCTTAAGG 
TCACCTTCAC TGCCATGCTT GTGCCAAAGG ATCACACCAC CACCTTCGCT GTCAGATCCA 
CTTAAAGAGC TCTTTCGGCA ACAGGAAGTA GTAAGGATGA AACTGCGTTT GCAACATAGT 
ATTGAAAGGG AAAAACTTAT TGTATCCAAT GAACAAGAAG TTCTGCGAGT TCATTACAGA 
GCTGCAAGAA CACTGGCCAA TCAAACACTG CCATTTAGTG CTTGTACTGT CTTACTGGAT 
GCAGAAGTGT ACAATGTGCC CTTGGACTCA CAGTCTGATG ACAGCAAAAC TTCTGTGAGA 
GATCGATTTA ATGCAAGACA GTTCATGTCC TGGTTGCAAG ATGTGGACGA TAAATTTGAC 
AAGCTAAAGA CCTGTCTCTT AATGAGGCAG CAACACGAAG CTGCAGCTTT AAATGCCGTC 
CAGAGGCTAG AATGGCAGCT CAAACTCCAG GAGCTGGACC CTGCCACGTA TAAGTCTCTC 
AGTATTTATG AAATCCAGGA GTTTTATGTT CCCCTTGTTG ATGTTAATGA TGATTTTGAA 
CTGACTCCTA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105278.1
CDS	538..6672
Translation	MPKSGFTKPVQSENSDSDSSMVERPYGRKSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSGDDESYTDSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTRRERISVDFLSSSTLRSPSLPCLCQRITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSLSIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_030249418.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249418.1

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGAAAAA 
GAAGGTCCAG AGAAGAAGAA AACAAAAAAA GAAGCTGGAA CTAAGAAGTC CACCCCAGTT 
AGCATTCTTT TTGGTTATCC ACTTTCTGAA CGAAAGCAGA TGGCACTCCT AATGCAGATG 
ACAGCAAGGG ACAACAGCCC AGATTCCACA CCAAGTCATC CGTCACAGGC AACGCCAGCC 
CAGAAGAAAA CGCCCAGCTC TTCATCTCGA CAGAAAGATA AAATTAATAA AAGAAATGAG 
CGTGGCGAAA CTCCTTTACA CATGGCCGCT ATTCGAGGAG ACGTGAAGCA AGTCAAAGAG 
TTAATTAGTT TAGGGGCAAA CGTCAATGTG AAGGACTTTG CAGGTTGGAC CCCTTTGCAT 
GAAGCTTGCA ATGTTGGCTA TTACGATGTT GCTAAGATAC TGATAGCAGC TGGCGCAGAC 
GTTAACACAC AAGGACTGGA TGATGACACC CCGCTCCATG ACTCTGCCAG CAGTGGCCAT 
AGAGACATAG TGAAGTTGTT GCTTCGTCAT GGCGGGAATC CATTCCAAGC TAATAAACAT 
GGAGAACGGC CAGTGGATGT GGCAGAGACA GAGGAGTTGG AGTTGCTGCT AAAAAGAGAG 
GTGCCTTTGT CTGGCGATGA TGAAAGTTAT ACAGATTCAG AAGAGGCTCA GTCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCA GAGACAGAGA AAGACTCTTT CGTCTGTGGC 
AGTAAAATCC TCCCAAGCAA AGCCCCGCTG CCTTCTGCAC TGGATGAGTA TGAGTTCAAA 
GATGATGATG AAGAAGAGAT AAGTAAGATG ATTGACGACA GGCACATTCT TAGGAAAGAA 
CAGCGGAGAG AGAATGAATC TGAAGCAGAA AGGAGTGGTC TGTTTGCAAA ACAGGAGAAA 
ACCTTCTATT CTAAGTCATT TAAAACAAAA AAACAGAAGC CGTCAAGGGT TCTGTGCTCC 
AGTACTGAAA GCTCTGATGA AGAGGGTCTT CAGAACAGAA AGATTTCTAC TGCTTGTTCT 
GTTGCTGAAT CATCACATCC TGATATGCAA GCCAAAAAAG AATATGAATA CAAACAGAAA 
GGCAAAGTTA AAAGAAAATT AAAAAATCAG AATAAAAACA AAGAGAACCA AGAGCTGAAA 
CAAGAAAAAG AGGGGAAAGA AAATAGCAGG ATAGCAACCT TGACAGTTAA TACTGCGCTG 
GATTGTTCAG AAAAATACAG AGAGGAGGGG AACTTCAGGA AATCTTTTAG CCCAAAGGAT 
GATACTTCAT TACACTTATT TCATATTTCC ACTGGTAAAT CTCCCAAACA TTCCTGTGGA 
TTAAGTGAAA AACAGACACC ACTGAAGCAA GAACACACGA AAGCATGCAT ATCCCCAGGA 
AGTTCTGAAA TGTCATTACA GCCTGATCTC ACTCGGTATG ATAATGCAGA ACCTGAAAGT 
TCAAATGTCA AATCTTATAA GCACAAGGAA AAAAACAAAC AGCAGAAAGA TTTTCACATA 
GAGTTTGGTG AAAAATCAAA TGCCAAAATT AAAGATGAAG ATCATAATCC AGCATTTGAA 
AACTCAGACT GCACACTGAA GAAGATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAA 
TTAAAACATA AAGAGAAGGA TAAAGAAAAG CACAAAAAGG AAGTTGAAGG TGAAAAGGAA 
AAATATAAAA ATAAAGACAA TGCTAAAGAG CTACAGAGGA GCATCGAATT TGACAGAGAG 
TTTTGGAAAG AAAATTTTTT TAAAAGTGAT GAAACTGAGG ATCTCTTTTT AACTATGGAA 
CATGAGTCCA TAATAGAAAA AAAGACAAAA ATGGAAAAAA ACATGAAAGA TGATAGGTCA 
GCTAAAGAAA AGCATGTCTC AAAAGAAAGG AACTTTAAAG AAGAACGGGA AAAGACTAAA 
AAGGAAAATG AAAAATCTCT TAGGGAAGAA AAGGTAAAAG ATCCGAAAGA AGAAAAAGAC 
AGTGTATCCA CAGAGAAGGA GGCCGAGTGC TGTTCGGTGG GTGTCAGTGT GAGCCAGGAA 
GCTGCAGGGC TGCATTCATC AGAAAAGGAA ATGGATGCTG AGAAGCAAGA AAGACACACA 
AAAGAGAGGG AAAAGTCTGA TCGGCGCTTT CAGACTAAGG AGAAAGAGCT TGACAGGAAC 
GAGAGGAAAT TTTCTGAAAA AGAAAAGAAG ATAAAACATG AACATAAGTC AGAAAGAGAG 
AAGCTGGATT TCAGTGACTG CACTGACAGA GTGAGAGAGA AGGACAAGCT GTACTCACAC 
CAGACAGAAA GGTGCCACAA GGACAGCGAG AAGCTGAAAA ACACTGTCAG AAAGACTGAT 
GACAGAGAGA GGAACAGAGA AAAGGCGGAT AGAAAGCACG ACAGAGAGAA ACCTGAACGA 
GAGAGGTACT TAGCAGAAAA CAAAGAAAAG CACTTAGAGA AAAGAAAGCA ATCTGACAGC 
AGTGAGTATT CCAAATCAGA GAAGGTCAGA ACTAAAGACA GAGACAGGGA AGGAGAGAAG 
AAGGAGAAAC TTAGAGATAA AGAAAGTGTA AATGTAACTA ACTTGAAACA TTTGCAGGAA 
GAGAGAAAGT CCAGTACAGG AGACAGCAGT GGTAAAACCC AGCATGAGAA AGCTCTGTCC 
CTTAAAGAAA GGGCAAGAGA TGAGCCTTTG AAAACTCCAG ATGGAAAAGA GAAAGATAAA 
AAGGATATAG ACAGATACAA AGAGCGAGAC AAGCGTAAAG ATAAAGCCCA GCTAAATACT 
TTAATCAGAC TAAAATCTGA AACAGAGAAG CTTAAACCTA AATTATCACC AGCATCAAAA 
GATACTCGAC CTAAAGAAAA GAGGTTAGTA AATGATGATT TAATGCAGAC AAGTTTTGAA 
CGAATGCTTA GCCTTAAAGA CCTAGAAATA GAACAGTGGC ACAGAAAACA TAAGGAAAAA 
ATTAAGCAAA AGGAAAAAGA ACGATTACGC AATCGTACTT GTTTAGAACT TAAAGTAAAA 
GATAAAGAAA AAACAAAACA TGCACCCACA GAATCCAAAA GTAAAGAACT TACCAGGTCA 
AGAAGTTCAG AGTTGACTGA TGTTTATAGC AAGGAGAAAC AATCTAAAGA TGTTGGCAGT 
AATCGCTCAC AATCTGTTGA CAACAGAAAT GTATTGAATT TAGGAAAGCC ATCCTACATT 
TCAGAGAGTG GCTCCAACCG GTCTCCTAGG TCGGAAGGTG AGAGGCTGGG TCTTAGCTCT 
AGGTCTGTGT CCATGCTTTC TGTTGCTAGT TCAGAAGACT CTTGCCATAC AGTGACAACC 
CCAAGGCCTC TCGTTGAGTA TGACTCTGAC TTTATGTTAG AGGGCTCGGA ATCTCAGTTG 
TCATTTTCTC AGTCACCTTT TTTGCCAAAT GCCAAATCTC CAGCTCTTTA TGAAAGAGAG 
CTGGAGGGTC TCACTGAACT ACCAGACCGA CTTAAACCAC CATATACAAA CAGACTTCCA 
GTGTGCCATC TCAGGTCATC TTCTGTGGAA GATGTTAAAC TAATTACTAA TGAGGTAAGA 
CCTGCTGTAG AAATTAGAAG ATGCAGTATG CCCTCAGTTA TCTGTGAGCC CATAAAACAT 
TTCCAAGTAT CAGAAGAAAG CAGTCAAGGC AACTTAGCTG TTCCCAGAGA GACCTGTCTG 
TCTCCCAAGC CTGAAGTGTC TTCAGCAGTA CCTGAGAGGG GCCTTTCGCA TGTGTCTGAC 
TTACATCCTA ACTGTACAGG CTCTCCAACG AGGTCTGCAA ACAGCAGCTA TATTTCATCT 
GATGTAAATG TAATCAAGAG TGCAACTCTT GTGGGTACAC TGATTGACAG TTCGATGCAT 
TTGGAACCGT CTAATCAGGT TGGAGTGATT CCAAGTAAAT CATGGGAGAC GCCTGTTGAT 
AAACTGGAGT CTTTAAGCAC CAATGACTTC ATCTGTCCAG ATTCTAATAT ATCTGATCAA 
GATTCCTCTG CTCAGAGTTT TTATAATTCA GAAAACAAAA TGTTAAGAGA ACAAAATACT 
GACTGCTTAC CTCCGCACCA GCCTGAACTG CCAGGAAACT CATGTGCTCA GGACCCAGCA 
TCCTTTCTGT CTTCACCTCA GCCCTGCTCT TTTTCCAACC AACCACTGGC AGGTGCTGAC 
TCTGTTCCGA AACCCGTGTC TTTGTCATAT GTTGTCAGTC AAGAGCCAGG TGTTTTACAA 
CAGAAAAGTG CAGCTCAGAT GGCCAGTTCT GTTTTAGATA GTGATAATAA CTTTCCAGAG 
GATGTGGAAA ATACATTTGT CTTAGCAGAC ATTCAGAAGA CAAATTCTTT TTTACCAGCT 
TACTCTGAAA GCACTGTGCA AGAAGCACTC CCAAGCTTTG AGAAGGCCAA TACTTTGCTT 
GTGTTGCCAT CAGAAAAGGA CTTCAATGGG AGTGATACTG CTCAGCCAAA CACACATTAT 
GCATTTAGCA AACTCACATA CAAGTCTTCC AATAGCCAGG AAATTGAGAA GGGCCCAGAT 
GATACTCAAA TCATTTCACA TGAAAAGGAA AACAAATTAC AAAGTTTGGT CTTAACTCAG 
TTGAGTAAAT GTGATTCTGA TTTGTATGAG ATGAATGCAG GGCTGCCAAA AGGAAACCTG 
AATGAACAGG ATAACCCAAA GCATGGTCTT GACAGCGAAA AGTACTCGCT TTCCACTGAA 
GATGAAGAAT CTCAGCAGAG CACCTTATCA AGTGTGGAAA ACCATTCACA GCAGTCGGCC 
CAACCAGAGA TGCATAAATA TGACCAGCTT AGTAAAGTAG AACTAGAAGA AAATGCCGAA 
GACGATAAAA CTGAAAACCA GACCCCTCAA AGAGTGACTA GAAACAAAGC AAGCACAGTG 
GTCAATCCAA GCAAGCCCAT GCTTGCTGGC TGTGCCCTTG TAGCAGAGAA AGATAACGAG 
TCCTCCCCTA GGGGACGAAT CAGACTCACT GAAGAGGATG ATCCTCAGAT CCACCATCCT 
CGGAAGAGGA AAGTGTCACG GGTTCCTCAG CCTGTGCAAG TGAGCCCCTC TTTACTGCAA 
GCAAAGGAGA AAACTCAGCA GTCTCTAGCA GCCATTGTAG ATTCTCTGAA GTTAGATGAG 
ATTGAGCCAT ACAGTTCTGA GAGAGCAAAT CCATATTTTG AATATTTGCA CATAAGAAAA 
AAAATTGAAG AAAAACGCAA GTTGCTATGT AGCGTTATCC CTCAAGCTCC TCAGTATTAT 
GATGAATACG TAACGTTTAA TGGATCCTAT CTCCTGGATG GAAACCCCTT AAGCAAGATT 
TGTATTCCTA CAAGAAGGGA GAGAATCTCG GTTGATTTTC TCAGCTCATC AACCTTAAGG 
TCACCTTCAC TGCCATGCTT GTGCCAAAGG ATCACACCAC CACCTTCGCT GTCAGATCCA 
CTTAAAGAGC TCTTTCGGCA ACAGGAAGTA GTAAGGATGA AACTGCGTTT GCAACATAGT 
ATTGAAAGGG AAAAACTTAT TGTATCCAAT GAACAAGAAG TTCTGCGAGT TCATTACAGA 
GCTGCAAGAA CACTGGCCAA TCAAACACTG CCATTTAGTG CTTGTACTGT CTTACTGGAT 
GCAGAAGTGT ACAATGTGCC CTTGGACTCA CAGTCTGATG ACAGCAAAAC TTCTGTGAGA 
GATCGATTTA ATGCAAGACA GTTCATGTCC TGGTTGCAAG ATGTGGACGA TAAATTTGAC 
AAGCTAAAGA CCTGTCTCTT AATGAGGCAG CAACACGAAG CTGCAGCTTT AAATGCCGTC 
CAGAGGCTAG AATGGCAGCT CAAACTCCAG GAGCTGGACC CTGCCACGTA TAAGTCTCTC 
AGTATTTATG AAATCCAGGA GTTTTATGTT CCCCTTGTTG ATGTTAATGA TGATTTTGAA 
CTGACTCCTA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( XM_006523397.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523460.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X5, mRNA.

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CloneID	OMu52955
Clone ID Related Accession (Same CDS sequence)	XM_030249418.1 , XM_006523397.4 , XM_030249417.1 , XM_006523397.3
Accession Version	XM_006523397.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523397.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGAAAAA 
GAAGGTCCAG AGAAGAAGAA AACAAAAAAA GAAGCTGGAA CTAAGAAGTC CACCCCAGTT 
AGCATTCTTT TTGGTTATCC ACTTTCTGAA CGAAAGCAGA TGGCACTCCT AATGCAGATG 
ACAGCAAGGG ACAACAGCCC AGATTCCACA CCAAGTCATC CGTCACAGGC AACGCCAGCC 
CAGAAGAAAA CGCCCAGCTC TTCATCTCGA CAGAAAGATA AAATTAATAA AAGAAATGAG 
CGTGGCGAAA CTCCTTTACA CATGGCCGCT ATTCGAGGAG ACGTGAAGCA AGTCAAAGAG 
TTAATTAGTT TAGGGGCAAA CGTCAATGTG AAGGACTTTG CAGGTTGGAC CCCTTTGCAT 
GAAGCTTGCA ATGTTGGCTA TTACGATGTT GCTAAGATAC TGATAGCAGC TGGCGCAGAC 
GTTAACACAC AAGGACTGGA TGATGACACC CCGCTCCATG ACTCTGCCAG CAGTGGCCAT 
AGAGACATAG TGAAGTTGTT GCTTCGTCAT GGCGGGAATC CATTCCAAGC TAATAAACAT 
GGAGAACGGC CAGTGGATGT GGCAGAGACA GAGGAGTTGG AGTTGCTGCT AAAAAGAGAG 
GTGCCTTTGT CTGGCGATGA TGAAAGTTAT ACAGATTCAG AAGAGGCTCA GTCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCA GAGACAGAGA AAGACTCTTT CGTCTGTGGC 
AGTAAAATCC TCCCAAGCAA AGCCCCGCTG CCTTCTGCAC TGGATGAGTA TGAGTTCAAA 
GATGATGATG AAGAAGAGAT AAGTAAGATG ATTGACGACA GGCACATTCT TAGGAAAGAA 
CAGCGGAGAG AGAATGAATC TGAAGCAGAA AGGAGTGGTC TGTTTGCAAA ACAGGAGAAA 
ACCTTCTATT CTAAGTCATT TAAAACAAAA AAACAGAAGC CGTCAAGGGT TCTGTGCTCC 
AGTACTGAAA GCTCTGATGA AGAGGGTCTT CAGAACAGAA AGATTTCTAC TGCTTGTTCT 
GTTGCTGAAT CATCACATCC TGATATGCAA GCCAAAAAAG AATATGAATA CAAACAGAAA 
GGCAAAGTTA AAAGAAAATT AAAAAATCAG AATAAAAACA AAGAGAACCA AGAGCTGAAA 
CAAGAAAAAG AGGGGAAAGA AAATAGCAGG ATAGCAACCT TGACAGTTAA TACTGCGCTG 
GATTGTTCAG AAAAATACAG AGAGGAGGGG AACTTCAGGA AATCTTTTAG CCCAAAGGAT 
GATACTTCAT TACACTTATT TCATATTTCC ACTGGTAAAT CTCCCAAACA TTCCTGTGGA 
TTAAGTGAAA AACAGACACC ACTGAAGCAA GAACACACGA AAGCATGCAT ATCCCCAGGA 
AGTTCTGAAA TGTCATTACA GCCTGATCTC ACTCGGTATG ATAATGCAGA ACCTGAAAGT 
TCAAATGTCA AATCTTATAA GCACAAGGAA AAAAACAAAC AGCAGAAAGA TTTTCACATA 
GAGTTTGGTG AAAAATCAAA TGCCAAAATT AAAGATGAAG ATCATAATCC AGCATTTGAA 
AACTCAGACT GCACACTGAA GAAGATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAA 
TTAAAACATA AAGAGAAGGA TAAAGAAAAG CACAAAAAGG AAGTTGAAGG TGAAAAGGAA 
AAATATAAAA ATAAAGACAA TGCTAAAGAG CTACAGAGGA GCATCGAATT TGACAGAGAG 
TTTTGGAAAG AAAATTTTTT TAAAAGTGAT GAAACTGAGG ATCTCTTTTT AACTATGGAA 
CATGAGTCCA TAATAGAAAA AAAGACAAAA ATGGAAAAAA ACATGAAAGA TGATAGGTCA 
GCTAAAGAAA AGCATGTCTC AAAAGAAAGG AACTTTAAAG AAGAACGGGA AAAGACTAAA 
AAGGAAAATG AAAAATCTCT TAGGGAAGAA AAGGTAAAAG ATCCGAAAGA AGAAAAAGAC 
AGTGTATCCA CAGAGAAGGA GGCCGAGTGC TGTTCGGTGG GTGTCAGTGT GAGCCAGGAA 
GCTGCAGGGC TGCATTCATC AGAAAAGGAA ATGGATGCTG AGAAGCAAGA AAGACACACA 
AAAGAGAGGG AAAAGTCTGA TCGGCGCTTT CAGACTAAGG AGAAAGAGCT TGACAGGAAC 
GAGAGGAAAT TTTCTGAAAA AGAAAAGAAG ATAAAACATG AACATAAGTC AGAAAGAGAG 
AAGCTGGATT TCAGTGACTG CACTGACAGA GTGAGAGAGA AGGACAAGCT GTACTCACAC 
CAGACAGAAA GGTGCCACAA GGACAGCGAG AAGCTGAAAA ACACTGTCAG AAAGACTGAT 
GACAGAGAGA GGAACAGAGA AAAGGCGGAT AGAAAGCACG ACAGAGAGAA ACCTGAACGA 
GAGAGGTACT TAGCAGAAAA CAAAGAAAAG CACTTAGAGA AAAGAAAGCA ATCTGACAGC 
AGTGAGTATT CCAAATCAGA GAAGGTCAGA ACTAAAGACA GAGACAGGGA AGGAGAGAAG 
AAGGAGAAAC TTAGAGATAA AGAAAGTGTA AATGTAACTA ACTTGAAACA TTTGCAGGAA 
GAGAGAAAGT CCAGTACAGG AGACAGCAGT GGTAAAACCC AGCATGAGAA AGCTCTGTCC 
CTTAAAGAAA GGGCAAGAGA TGAGCCTTTG AAAACTCCAG ATGGAAAAGA GAAAGATAAA 
AAGGATATAG ACAGATACAA AGAGCGAGAC AAGCGTAAAG ATAAAGCCCA GCTAAATACT 
TTAATCAGAC TAAAATCTGA AACAGAGAAG CTTAAACCTA AATTATCACC AGCATCAAAA 
GATACTCGAC CTAAAGAAAA GAGGTTAGTA AATGATGATT TAATGCAGAC AAGTTTTGAA 
CGAATGCTTA GCCTTAAAGA CCTAGAAATA GAACAGTGGC ACAGAAAACA TAAGGAAAAA 
ATTAAGCAAA AGGAAAAAGA ACGATTACGC AATCGTACTT GTTTAGAACT TAAAGTAAAA 
GATAAAGAAA AAACAAAACA TGCACCCACA GAATCCAAAA GTAAAGAACT TACCAGGTCA 
AGAAGTTCAG AGTTGACTGA TGTTTATAGC AAGGAGAAAC AATCTAAAGA TGTTGGCAGT 
AATCGCTCAC AATCTGTTGA CAACAGAAAT GTATTGAATT TAGGAAAGCC ATCCTACATT 
TCAGAGAGTG GCTCCAACCG GTCTCCTAGG TCGGAAGGTG AGAGGCTGGG TCTTAGCTCT 
AGGTCTGTGT CCATGCTTTC TGTTGCTAGT TCAGAAGACT CTTGCCATAC AGTGACAACC 
CCAAGGCCTC TCGTTGAGTA TGACTCTGAC TTTATGTTAG AGGGCTCGGA ATCTCAGTTG 
TCATTTTCTC AGTCACCTTT TTTGCCAAAT GCCAAATCTC CAGCTCTTTA TGAAAGAGAG 
CTGGAGGGTC TCACTGAACT ACCAGACCGA CTTAAACCAC CATATACAAA CAGACTTCCA 
GTGTGCCATC TCAGGTCATC TTCTGTGGAA GATGTTAAAC TAATTACTAA TGAGGTAAGA 
CCTGCTGTAG AAATTAGAAG ATGCAGTATG CCCTCAGTTA TCTGTGAGCC CATAAAACAT 
TTCCAAGTAT CAGAAGAAAG CAGTCAAGGC AACTTAGCTG TTCCCAGAGA GACCTGTCTG 
TCTCCCAAGC CTGAAGTGTC TTCAGCAGTA CCTGAGAGGG GCCTTTCGCA TGTGTCTGAC 
TTACATCCTA ACTGTACAGG CTCTCCAACG AGGTCTGCAA ACAGCAGCTA TATTTCATCT 
GATGTAAATG TAATCAAGAG TGCAACTCTT GTGGGTACAC TGATTGACAG TTCGATGCAT 
TTGGAACCGT CTAATCAGGT TGGAGTGATT CCAAGTAAAT CATGGGAGAC GCCTGTTGAT 
AAACTGGAGT CTTTAAGCAC CAATGACTTC ATCTGTCCAG ATTCTAATAT ATCTGATCAA 
GATTCCTCTG CTCAGAGTTT TTATAATTCA GAAAACAAAA TGTTAAGAGA ACAAAATACT 
GACTGCTTAC CTCCGCACCA GCCTGAACTG CCAGGAAACT CATGTGCTCA GGACCCAGCA 
TCCTTTCTGT CTTCACCTCA GCCCTGCTCT TTTTCCAACC AACCACTGGC AGGTGCTGAC 
TCTGTTCCGA AACCCGTGTC TTTGTCATAT GTTGTCAGTC AAGAGCCAGG TGTTTTACAA 
CAGAAAAGTG CAGCTCAGAT GGCCAGTTCT GTTTTAGATA GTGATAATAA CTTTCCAGAG 
GATGTGGAAA ATACATTTGT CTTAGCAGAC ATTCAGAAGA CAAATTCTTT TTTACCAGCT 
TACTCTGAAA GCACTGTGCA AGAAGCACTC CCAAGCTTTG AGAAGGCCAA TACTTTGCTT 
GTGTTGCCAT CAGAAAAGGA CTTCAATGGG AGTGATACTG CTCAGCCAAA CACACATTAT 
GCATTTAGCA AACTCACATA CAAGTCTTCC AATAGCCAGG AAATTGAGAA GGGCCCAGAT 
GATACTCAAA TCATTTCACA TGAAAAGGAA AACAAATTAC AAAGTTTGGT CTTAACTCAG 
TTGAGTAAAT GTGATTCTGA TTTGTATGAG ATGAATGCAG GGCTGCCAAA AGGAAACCTG 
AATGAACAGG ATAACCCAAA GCATGGTCTT GACAGCGAAA AGTACTCGCT TTCCACTGAA 
GATGAAGAAT CTCAGCAGAG CACCTTATCA AGTGTGGAAA ACCATTCACA GCAGTCGGCC 
CAACCAGAGA TGCATAAATA TGACCAGCTT AGTAAAGTAG AACTAGAAGA AAATGCCGAA 
GACGATAAAA CTGAAAACCA GACCCCTCAA AGAGTGACTA GAAACAAAGC AAGCACAGTG 
GTCAATCCAA GCAAGCCCAT GCTTGCTGGC TGTGCCCTTG TAGCAGAGAA AGATAACGAG 
TCCTCCCCTA GGGGACGAAT CAGACTCACT GAAGAGGATG ATCCTCAGAT CCACCATCCT 
CGGAAGAGGA AAGTGTCACG GGTTCCTCAG CCTGTGCAAG TGAGCCCCTC TTTACTGCAA 
GCAAAGGAGA AAACTCAGCA GTCTCTAGCA GCCATTGTAG ATTCTCTGAA GTTAGATGAG 
ATTGAGCCAT ACAGTTCTGA GAGAGCAAAT CCATATTTTG AATATTTGCA CATAAGAAAA 
AAAATTGAAG AAAAACGCAA GTTGCTATGT AGCGTTATCC CTCAAGCTCC TCAGTATTAT 
GATGAATACG TAACGTTTAA TGGATCCTAT CTCCTGGATG GAAACCCCTT AAGCAAGATT 
TGTATTCCTA CAAGAAGGGA GAGAATCTCG GTTGATTTTC TCAGCTCATC AACCTTAAGG 
TCACCTTCAC TGCCATGCTT GTGCCAAAGG ATCACACCAC CACCTTCGCT GTCAGATCCA 
CTTAAAGAGC TCTTTCGGCA ACAGGAAGTA GTAAGGATGA AACTGCGTTT GCAACATAGT 
ATTGAAAGGG AAAAACTTAT TGTATCCAAT GAACAAGAAG TTCTGCGAGT TCATTACAGA 
GCTGCAAGAA CACTGGCCAA TCAAACACTG CCATTTAGTG CTTGTACTGT CTTACTGGAT 
GCAGAAGTGT ACAATGTGCC CTTGGACTCA CAGTCTGATG ACAGCAAAAC TTCTGTGAGA 
GATCGATTTA ATGCAAGACA GTTCATGTCC TGGTTGCAAG ATGTGGACGA TAAATTTGAC 
AAGCTAAAGA CCTGTCTCTT AATGAGGCAG CAACACGAAG CTGCAGCTTT AAATGCCGTC 
CAGAGGCTAG AATGGCAGCT CAAACTCCAG GAGCTGGACC CTGCCACGTA TAAGTCTCTC 
AGTATTTATG AAATCCAGGA GTTTTATGTT CCCCTTGTTG ATGTTAATGA TGATTTTGAA 
CTGACTCCTA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523460.1
CDS	269..6403
Translation	MPKSGFTKPVQSENSDSDSSMVERPYGRKSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSGDDESYTDSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTRRERISVDFLSSSTLRSPSLPCLCQRITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSLSIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_006523397.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523397.4

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGAAAAA 
GAAGGTCCAG AGAAGAAGAA AACAAAAAAA GAAGCTGGAA CTAAGAAGTC CACCCCAGTT 
AGCATTCTTT TTGGTTATCC ACTTTCTGAA CGAAAGCAGA TGGCACTCCT AATGCAGATG 
ACAGCAAGGG ACAACAGCCC AGATTCCACA CCAAGTCATC CGTCACAGGC AACGCCAGCC 
CAGAAGAAAA CGCCCAGCTC TTCATCTCGA CAGAAAGATA AAATTAATAA AAGAAATGAG 
CGTGGCGAAA CTCCTTTACA CATGGCCGCT ATTCGAGGAG ACGTGAAGCA AGTCAAAGAG 
TTAATTAGTT TAGGGGCAAA CGTCAATGTG AAGGACTTTG CAGGTTGGAC CCCTTTGCAT 
GAAGCTTGCA ATGTTGGCTA TTACGATGTT GCTAAGATAC TGATAGCAGC TGGCGCAGAC 
GTTAACACAC AAGGACTGGA TGATGACACC CCGCTCCATG ACTCTGCCAG CAGTGGCCAT 
AGAGACATAG TGAAGTTGTT GCTTCGTCAT GGCGGGAATC CATTCCAAGC TAATAAACAT 
GGAGAACGGC CAGTGGATGT GGCAGAGACA GAGGAGTTGG AGTTGCTGCT AAAAAGAGAG 
GTGCCTTTGT CTGGCGATGA TGAAAGTTAT ACAGATTCAG AAGAGGCTCA GTCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCA GAGACAGAGA AAGACTCTTT CGTCTGTGGC 
AGTAAAATCC TCCCAAGCAA AGCCCCGCTG CCTTCTGCAC TGGATGAGTA TGAGTTCAAA 
GATGATGATG AAGAAGAGAT AAGTAAGATG ATTGACGACA GGCACATTCT TAGGAAAGAA 
CAGCGGAGAG AGAATGAATC TGAAGCAGAA AGGAGTGGTC TGTTTGCAAA ACAGGAGAAA 
ACCTTCTATT CTAAGTCATT TAAAACAAAA AAACAGAAGC CGTCAAGGGT TCTGTGCTCC 
AGTACTGAAA GCTCTGATGA AGAGGGTCTT CAGAACAGAA AGATTTCTAC TGCTTGTTCT 
GTTGCTGAAT CATCACATCC TGATATGCAA GCCAAAAAAG AATATGAATA CAAACAGAAA 
GGCAAAGTTA AAAGAAAATT AAAAAATCAG AATAAAAACA AAGAGAACCA AGAGCTGAAA 
CAAGAAAAAG AGGGGAAAGA AAATAGCAGG ATAGCAACCT TGACAGTTAA TACTGCGCTG 
GATTGTTCAG AAAAATACAG AGAGGAGGGG AACTTCAGGA AATCTTTTAG CCCAAAGGAT 
GATACTTCAT TACACTTATT TCATATTTCC ACTGGTAAAT CTCCCAAACA TTCCTGTGGA 
TTAAGTGAAA AACAGACACC ACTGAAGCAA GAACACACGA AAGCATGCAT ATCCCCAGGA 
AGTTCTGAAA TGTCATTACA GCCTGATCTC ACTCGGTATG ATAATGCAGA ACCTGAAAGT 
TCAAATGTCA AATCTTATAA GCACAAGGAA AAAAACAAAC AGCAGAAAGA TTTTCACATA 
GAGTTTGGTG AAAAATCAAA TGCCAAAATT AAAGATGAAG ATCATAATCC AGCATTTGAA 
AACTCAGACT GCACACTGAA GAAGATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAA 
TTAAAACATA AAGAGAAGGA TAAAGAAAAG CACAAAAAGG AAGTTGAAGG TGAAAAGGAA 
AAATATAAAA ATAAAGACAA TGCTAAAGAG CTACAGAGGA GCATCGAATT TGACAGAGAG 
TTTTGGAAAG AAAATTTTTT TAAAAGTGAT GAAACTGAGG ATCTCTTTTT AACTATGGAA 
CATGAGTCCA TAATAGAAAA AAAGACAAAA ATGGAAAAAA ACATGAAAGA TGATAGGTCA 
GCTAAAGAAA AGCATGTCTC AAAAGAAAGG AACTTTAAAG AAGAACGGGA AAAGACTAAA 
AAGGAAAATG AAAAATCTCT TAGGGAAGAA AAGGTAAAAG ATCCGAAAGA AGAAAAAGAC 
AGTGTATCCA CAGAGAAGGA GGCCGAGTGC TGTTCGGTGG GTGTCAGTGT GAGCCAGGAA 
GCTGCAGGGC TGCATTCATC AGAAAAGGAA ATGGATGCTG AGAAGCAAGA AAGACACACA 
AAAGAGAGGG AAAAGTCTGA TCGGCGCTTT CAGACTAAGG AGAAAGAGCT TGACAGGAAC 
GAGAGGAAAT TTTCTGAAAA AGAAAAGAAG ATAAAACATG AACATAAGTC AGAAAGAGAG 
AAGCTGGATT TCAGTGACTG CACTGACAGA GTGAGAGAGA AGGACAAGCT GTACTCACAC 
CAGACAGAAA GGTGCCACAA GGACAGCGAG AAGCTGAAAA ACACTGTCAG AAAGACTGAT 
GACAGAGAGA GGAACAGAGA AAAGGCGGAT AGAAAGCACG ACAGAGAGAA ACCTGAACGA 
GAGAGGTACT TAGCAGAAAA CAAAGAAAAG CACTTAGAGA AAAGAAAGCA ATCTGACAGC 
AGTGAGTATT CCAAATCAGA GAAGGTCAGA ACTAAAGACA GAGACAGGGA AGGAGAGAAG 
AAGGAGAAAC TTAGAGATAA AGAAAGTGTA AATGTAACTA ACTTGAAACA TTTGCAGGAA 
GAGAGAAAGT CCAGTACAGG AGACAGCAGT GGTAAAACCC AGCATGAGAA AGCTCTGTCC 
CTTAAAGAAA GGGCAAGAGA TGAGCCTTTG AAAACTCCAG ATGGAAAAGA GAAAGATAAA 
AAGGATATAG ACAGATACAA AGAGCGAGAC AAGCGTAAAG ATAAAGCCCA GCTAAATACT 
TTAATCAGAC TAAAATCTGA AACAGAGAAG CTTAAACCTA AATTATCACC AGCATCAAAA 
GATACTCGAC CTAAAGAAAA GAGGTTAGTA AATGATGATT TAATGCAGAC AAGTTTTGAA 
CGAATGCTTA GCCTTAAAGA CCTAGAAATA GAACAGTGGC ACAGAAAACA TAAGGAAAAA 
ATTAAGCAAA AGGAAAAAGA ACGATTACGC AATCGTACTT GTTTAGAACT TAAAGTAAAA 
GATAAAGAAA AAACAAAACA TGCACCCACA GAATCCAAAA GTAAAGAACT TACCAGGTCA 
AGAAGTTCAG AGTTGACTGA TGTTTATAGC AAGGAGAAAC AATCTAAAGA TGTTGGCAGT 
AATCGCTCAC AATCTGTTGA CAACAGAAAT GTATTGAATT TAGGAAAGCC ATCCTACATT 
TCAGAGAGTG GCTCCAACCG GTCTCCTAGG TCGGAAGGTG AGAGGCTGGG TCTTAGCTCT 
AGGTCTGTGT CCATGCTTTC TGTTGCTAGT TCAGAAGACT CTTGCCATAC AGTGACAACC 
CCAAGGCCTC TCGTTGAGTA TGACTCTGAC TTTATGTTAG AGGGCTCGGA ATCTCAGTTG 
TCATTTTCTC AGTCACCTTT TTTGCCAAAT GCCAAATCTC CAGCTCTTTA TGAAAGAGAG 
CTGGAGGGTC TCACTGAACT ACCAGACCGA CTTAAACCAC CATATACAAA CAGACTTCCA 
GTGTGCCATC TCAGGTCATC TTCTGTGGAA GATGTTAAAC TAATTACTAA TGAGGTAAGA 
CCTGCTGTAG AAATTAGAAG ATGCAGTATG CCCTCAGTTA TCTGTGAGCC CATAAAACAT 
TTCCAAGTAT CAGAAGAAAG CAGTCAAGGC AACTTAGCTG TTCCCAGAGA GACCTGTCTG 
TCTCCCAAGC CTGAAGTGTC TTCAGCAGTA CCTGAGAGGG GCCTTTCGCA TGTGTCTGAC 
TTACATCCTA ACTGTACAGG CTCTCCAACG AGGTCTGCAA ACAGCAGCTA TATTTCATCT 
GATGTAAATG TAATCAAGAG TGCAACTCTT GTGGGTACAC TGATTGACAG TTCGATGCAT 
TTGGAACCGT CTAATCAGGT TGGAGTGATT CCAAGTAAAT CATGGGAGAC GCCTGTTGAT 
AAACTGGAGT CTTTAAGCAC CAATGACTTC ATCTGTCCAG ATTCTAATAT ATCTGATCAA 
GATTCCTCTG CTCAGAGTTT TTATAATTCA GAAAACAAAA TGTTAAGAGA ACAAAATACT 
GACTGCTTAC CTCCGCACCA GCCTGAACTG CCAGGAAACT CATGTGCTCA GGACCCAGCA 
TCCTTTCTGT CTTCACCTCA GCCCTGCTCT TTTTCCAACC AACCACTGGC AGGTGCTGAC 
TCTGTTCCGA AACCCGTGTC TTTGTCATAT GTTGTCAGTC AAGAGCCAGG TGTTTTACAA 
CAGAAAAGTG CAGCTCAGAT GGCCAGTTCT GTTTTAGATA GTGATAATAA CTTTCCAGAG 
GATGTGGAAA ATACATTTGT CTTAGCAGAC ATTCAGAAGA CAAATTCTTT TTTACCAGCT 
TACTCTGAAA GCACTGTGCA AGAAGCACTC CCAAGCTTTG AGAAGGCCAA TACTTTGCTT 
GTGTTGCCAT CAGAAAAGGA CTTCAATGGG AGTGATACTG CTCAGCCAAA CACACATTAT 
GCATTTAGCA AACTCACATA CAAGTCTTCC AATAGCCAGG AAATTGAGAA GGGCCCAGAT 
GATACTCAAA TCATTTCACA TGAAAAGGAA AACAAATTAC AAAGTTTGGT CTTAACTCAG 
TTGAGTAAAT GTGATTCTGA TTTGTATGAG ATGAATGCAG GGCTGCCAAA AGGAAACCTG 
AATGAACAGG ATAACCCAAA GCATGGTCTT GACAGCGAAA AGTACTCGCT TTCCACTGAA 
GATGAAGAAT CTCAGCAGAG CACCTTATCA AGTGTGGAAA ACCATTCACA GCAGTCGGCC 
CAACCAGAGA TGCATAAATA TGACCAGCTT AGTAAAGTAG AACTAGAAGA AAATGCCGAA 
GACGATAAAA CTGAAAACCA GACCCCTCAA AGAGTGACTA GAAACAAAGC AAGCACAGTG 
GTCAATCCAA GCAAGCCCAT GCTTGCTGGC TGTGCCCTTG TAGCAGAGAA AGATAACGAG 
TCCTCCCCTA GGGGACGAAT CAGACTCACT GAAGAGGATG ATCCTCAGAT CCACCATCCT 
CGGAAGAGGA AAGTGTCACG GGTTCCTCAG CCTGTGCAAG TGAGCCCCTC TTTACTGCAA 
GCAAAGGAGA AAACTCAGCA GTCTCTAGCA GCCATTGTAG ATTCTCTGAA GTTAGATGAG 
ATTGAGCCAT ACAGTTCTGA GAGAGCAAAT CCATATTTTG AATATTTGCA CATAAGAAAA 
AAAATTGAAG AAAAACGCAA GTTGCTATGT AGCGTTATCC CTCAAGCTCC TCAGTATTAT 
GATGAATACG TAACGTTTAA TGGATCCTAT CTCCTGGATG GAAACCCCTT AAGCAAGATT 
TGTATTCCTA CAAGAAGGGA GAGAATCTCG GTTGATTTTC TCAGCTCATC AACCTTAAGG 
TCACCTTCAC TGCCATGCTT GTGCCAAAGG ATCACACCAC CACCTTCGCT GTCAGATCCA 
CTTAAAGAGC TCTTTCGGCA ACAGGAAGTA GTAAGGATGA AACTGCGTTT GCAACATAGT 
ATTGAAAGGG AAAAACTTAT TGTATCCAAT GAACAAGAAG TTCTGCGAGT TCATTACAGA 
GCTGCAAGAA CACTGGCCAA TCAAACACTG CCATTTAGTG CTTGTACTGT CTTACTGGAT 
GCAGAAGTGT ACAATGTGCC CTTGGACTCA CAGTCTGATG ACAGCAAAAC TTCTGTGAGA 
GATCGATTTA ATGCAAGACA GTTCATGTCC TGGTTGCAAG ATGTGGACGA TAAATTTGAC 
AAGCTAAAGA CCTGTCTCTT AATGAGGCAG CAACACGAAG CTGCAGCTTT AAATGCCGTC 
CAGAGGCTAG AATGGCAGCT CAAACTCCAG GAGCTGGACC CTGCCACGTA TAAGTCTCTC 
AGTATTTATG AAATCCAGGA GTTTTATGTT CCCCTTGTTG ATGTTAATGA TGATTTTGAA 
CTGACTCCTA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( XM_030249417.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105277.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X6, mRNA.

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CloneID	OMu52955
Clone ID Related Accession (Same CDS sequence)	XM_030249418.1 , XM_006523397.4 , XM_030249417.1 , XM_006523397.3
Accession Version	XM_030249417.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGAAAAA 
GAAGGTCCAG AGAAGAAGAA AACAAAAAAA GAAGCTGGAA CTAAGAAGTC CACCCCAGTT 
AGCATTCTTT TTGGTTATCC ACTTTCTGAA CGAAAGCAGA TGGCACTCCT AATGCAGATG 
ACAGCAAGGG ACAACAGCCC AGATTCCACA CCAAGTCATC CGTCACAGGC AACGCCAGCC 
CAGAAGAAAA CGCCCAGCTC TTCATCTCGA CAGAAAGATA AAATTAATAA AAGAAATGAG 
CGTGGCGAAA CTCCTTTACA CATGGCCGCT ATTCGAGGAG ACGTGAAGCA AGTCAAAGAG 
TTAATTAGTT TAGGGGCAAA CGTCAATGTG AAGGACTTTG CAGGTTGGAC CCCTTTGCAT 
GAAGCTTGCA ATGTTGGCTA TTACGATGTT GCTAAGATAC TGATAGCAGC TGGCGCAGAC 
GTTAACACAC AAGGACTGGA TGATGACACC CCGCTCCATG ACTCTGCCAG CAGTGGCCAT 
AGAGACATAG TGAAGTTGTT GCTTCGTCAT GGCGGGAATC CATTCCAAGC TAATAAACAT 
GGAGAACGGC CAGTGGATGT GGCAGAGACA GAGGAGTTGG AGTTGCTGCT AAAAAGAGAG 
GTGCCTTTGT CTGGCGATGA TGAAAGTTAT ACAGATTCAG AAGAGGCTCA GTCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCA GAGACAGAGA AAGACTCTTT CGTCTGTGGC 
AGTAAAATCC TCCCAAGCAA AGCCCCGCTG CCTTCTGCAC TGGATGAGTA TGAGTTCAAA 
GATGATGATG AAGAAGAGAT AAGTAAGATG ATTGACGACA GGCACATTCT TAGGAAAGAA 
CAGCGGAGAG AGAATGAATC TGAAGCAGAA AGGAGTGGTC TGTTTGCAAA ACAGGAGAAA 
ACCTTCTATT CTAAGTCATT TAAAACAAAA AAACAGAAGC CGTCAAGGGT TCTGTGCTCC 
AGTACTGAAA GCTCTGATGA AGAGGGTCTT CAGAACAGAA AGATTTCTAC TGCTTGTTCT 
GTTGCTGAAT CATCACATCC TGATATGCAA GCCAAAAAAG AATATGAATA CAAACAGAAA 
GGCAAAGTTA AAAGAAAATT AAAAAATCAG AATAAAAACA AAGAGAACCA AGAGCTGAAA 
CAAGAAAAAG AGGGGAAAGA AAATAGCAGG ATAGCAACCT TGACAGTTAA TACTGCGCTG 
GATTGTTCAG AAAAATACAG AGAGGAGGGG AACTTCAGGA AATCTTTTAG CCCAAAGGAT 
GATACTTCAT TACACTTATT TCATATTTCC ACTGGTAAAT CTCCCAAACA TTCCTGTGGA 
TTAAGTGAAA AACAGACACC ACTGAAGCAA GAACACACGA AAGCATGCAT ATCCCCAGGA 
AGTTCTGAAA TGTCATTACA GCCTGATCTC ACTCGGTATG ATAATGCAGA ACCTGAAAGT 
TCAAATGTCA AATCTTATAA GCACAAGGAA AAAAACAAAC AGCAGAAAGA TTTTCACATA 
GAGTTTGGTG AAAAATCAAA TGCCAAAATT AAAGATGAAG ATCATAATCC AGCATTTGAA 
AACTCAGACT GCACACTGAA GAAGATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAA 
TTAAAACATA AAGAGAAGGA TAAAGAAAAG CACAAAAAGG AAGTTGAAGG TGAAAAGGAA 
AAATATAAAA ATAAAGACAA TGCTAAAGAG CTACAGAGGA GCATCGAATT TGACAGAGAG 
TTTTGGAAAG AAAATTTTTT TAAAAGTGAT GAAACTGAGG ATCTCTTTTT AACTATGGAA 
CATGAGTCCA TAATAGAAAA AAAGACAAAA ATGGAAAAAA ACATGAAAGA TGATAGGTCA 
GCTAAAGAAA AGCATGTCTC AAAAGAAAGG AACTTTAAAG AAGAACGGGA AAAGACTAAA 
AAGGAAAATG AAAAATCTCT TAGGGAAGAA AAGGTAAAAG ATCCGAAAGA AGAAAAAGAC 
AGTGTATCCA CAGAGAAGGA GGCCGAGTGC TGTTCGGTGG GTGTCAGTGT GAGCCAGGAA 
GCTGCAGGGC TGCATTCATC AGAAAAGGAA ATGGATGCTG AGAAGCAAGA AAGACACACA 
AAAGAGAGGG AAAAGTCTGA TCGGCGCTTT CAGACTAAGG AGAAAGAGCT TGACAGGAAC 
GAGAGGAAAT TTTCTGAAAA AGAAAAGAAG ATAAAACATG AACATAAGTC AGAAAGAGAG 
AAGCTGGATT TCAGTGACTG CACTGACAGA GTGAGAGAGA AGGACAAGCT GTACTCACAC 
CAGACAGAAA GGTGCCACAA GGACAGCGAG AAGCTGAAAA ACACTGTCAG AAAGACTGAT 
GACAGAGAGA GGAACAGAGA AAAGGCGGAT AGAAAGCACG ACAGAGAGAA ACCTGAACGA 
GAGAGGTACT TAGCAGAAAA CAAAGAAAAG CACTTAGAGA AAAGAAAGCA ATCTGACAGC 
AGTGAGTATT CCAAATCAGA GAAGGTCAGA ACTAAAGACA GAGACAGGGA AGGAGAGAAG 
AAGGAGAAAC TTAGAGATAA AGAAAGTGTA AATGTAACTA ACTTGAAACA TTTGCAGGAA 
GAGAGAAAGT CCAGTACAGG AGACAGCAGT GGTAAAACCC AGCATGAGAA AGCTCTGTCC 
CTTAAAGAAA GGGCAAGAGA TGAGCCTTTG AAAACTCCAG ATGGAAAAGA GAAAGATAAA 
AAGGATATAG ACAGATACAA AGAGCGAGAC AAGCGTAAAG ATAAAGCCCA GCTAAATACT 
TTAATCAGAC TAAAATCTGA AACAGAGAAG CTTAAACCTA AATTATCACC AGCATCAAAA 
GATACTCGAC CTAAAGAAAA GAGGTTAGTA AATGATGATT TAATGCAGAC AAGTTTTGAA 
CGAATGCTTA GCCTTAAAGA CCTAGAAATA GAACAGTGGC ACAGAAAACA TAAGGAAAAA 
ATTAAGCAAA AGGAAAAAGA ACGATTACGC AATCGTACTT GTTTAGAACT TAAAGTAAAA 
GATAAAGAAA AAACAAAACA TGCACCCACA GAATCCAAAA GTAAAGAACT TACCAGGTCA 
AGAAGTTCAG AGTTGACTGA TGTTTATAGC AAGGAGAAAC AATCTAAAGA TGTTGGCAGT 
AATCGCTCAC AATCTGTTGA CAACAGAAAT GTATTGAATT TAGGAAAGCC ATCCTACATT 
TCAGAGAGTG GCTCCAACCG GTCTCCTAGG TCGGAAGGTG AGAGGCTGGG TCTTAGCTCT 
AGGTCTGTGT CCATGCTTTC TGTTGCTAGT TCAGAAGACT CTTGCCATAC AGTGACAACC 
CCAAGGCCTC TCGTTGAGTA TGACTCTGAC TTTATGTTAG AGGGCTCGGA ATCTCAGTTG 
TCATTTTCTC AGTCACCTTT TTTGCCAAAT GCCAAATCTC CAGCTCTTTA TGAAAGAGAG 
CTGGAGGGTC TCACTGAACT ACCAGACCGA CTTAAACCAC CATATACAAA CAGACTTCCA 
GTGTGCCATC TCAGGTCATC TTCTGTGGAA GATGTTAAAC TAATTACTAA TGAGGTAAGA 
CCTGCTGTAG AAATTAGAAG ATGCAGTATG CCCTCAGTTA TCTGTGAGCC CATAAAACAT 
TTCCAAGTAT CAGAAGAAAG CAGTCAAGGC AACTTAGCTG TTCCCAGAGA GACCTGTCTG 
TCTCCCAAGC CTGAAGTGTC TTCAGCAGTA CCTGAGAGGG GCCTTTCGCA TGTGTCTGAC 
TTACATCCTA ACTGTACAGG CTCTCCAACG AGGTCTGCAA ACAGCAGCTA TATTTCATCT 
GATGTAAATG TAATCAAGAG TGCAACTCTT GTGGGTACAC TGATTGACAG TTCGATGCAT 
TTGGAACCGT CTAATCAGGT TGGAGTGATT CCAAGTAAAT CATGGGAGAC GCCTGTTGAT 
AAACTGGAGT CTTTAAGCAC CAATGACTTC ATCTGTCCAG ATTCTAATAT ATCTGATCAA 
GATTCCTCTG CTCAGAGTTT TTATAATTCA GAAAACAAAA TGTTAAGAGA ACAAAATACT 
GACTGCTTAC CTCCGCACCA GCCTGAACTG CCAGGAAACT CATGTGCTCA GGACCCAGCA 
TCCTTTCTGT CTTCACCTCA GCCCTGCTCT TTTTCCAACC AACCACTGGC AGGTGCTGAC 
TCTGTTCCGA AACCCGTGTC TTTGTCATAT GTTGTCAGTC AAGAGCCAGG TGTTTTACAA 
CAGAAAAGTG CAGCTCAGAT GGCCAGTTCT GTTTTAGATA GTGATAATAA CTTTCCAGAG 
GATGTGGAAA ATACATTTGT CTTAGCAGAC ATTCAGAAGA CAAATTCTTT TTTACCAGCT 
TACTCTGAAA GCACTGTGCA AGAAGCACTC CCAAGCTTTG AGAAGGCCAA TACTTTGCTT 
GTGTTGCCAT CAGAAAAGGA CTTCAATGGG AGTGATACTG CTCAGCCAAA CACACATTAT 
GCATTTAGCA AACTCACATA CAAGTCTTCC AATAGCCAGG AAATTGAGAA GGGCCCAGAT 
GATACTCAAA TCATTTCACA TGAAAAGGAA AACAAATTAC AAAGTTTGGT CTTAACTCAG 
TTGAGTAAAT GTGATTCTGA TTTGTATGAG ATGAATGCAG GGCTGCCAAA AGGAAACCTG 
AATGAACAGG ATAACCCAAA GCATGGTCTT GACAGCGAAA AGTACTCGCT TTCCACTGAA 
GATGAAGAAT CTCAGCAGAG CACCTTATCA AGTGTGGAAA ACCATTCACA GCAGTCGGCC 
CAACCAGAGA TGCATAAATA TGACCAGCTT AGTAAAGTAG AACTAGAAGA AAATGCCGAA 
GACGATAAAA CTGAAAACCA GACCCCTCAA AGAGTGACTA GAAACAAAGC AAGCACAGTG 
GTCAATCCAA GCAAGCCCAT GCTTGCTGGC TGTGCCCTTG TAGCAGAGAA AGATAACGAG 
TCCTCCCCTA GGGGACGAAT CAGACTCACT GAAGAGGATG ATCCTCAGAT CCACCATCCT 
CGGAAGAGGA AAGTGTCACG GGTTCCTCAG CCTGTGCAAG TGAGCCCCTC TTTACTGCAA 
GCAAAGGAGA AAACTCAGCA GTCTCTAGCA GCCATTGTAG ATTCTCTGAA GTTAGATGAG 
ATTGAGCCAT ACAGTTCTGA GAGAGCAAAT CCATATTTTG AATATTTGCA CATAAGAAAA 
AAAATTGAAG AAAAACGCAA GTTGCTATGT AGCGTTATCC CTCAAGCTCC TCAGTATTAT 
GATGAATACG TAACGTTTAA TGGATCCTAT CTCCTGGATG GAAACCCCTT AAGCAAGATT 
TGTATTCCTA CAAGAAGGGA GAGAATCTCG GTTGATTTTC TCAGCTCATC AACCTTAAGG 
TCACCTTCAC TGCCATGCTT GTGCCAAAGG ATCACACCAC CACCTTCGCT GTCAGATCCA 
CTTAAAGAGC TCTTTCGGCA ACAGGAAGTA GTAAGGATGA AACTGCGTTT GCAACATAGT 
ATTGAAAGGG AAAAACTTAT TGTATCCAAT GAACAAGAAG TTCTGCGAGT TCATTACAGA 
GCTGCAAGAA CACTGGCCAA TCAAACACTG CCATTTAGTG CTTGTACTGT CTTACTGGAT 
GCAGAAGTGT ACAATGTGCC CTTGGACTCA CAGTCTGATG ACAGCAAAAC TTCTGTGAGA 
GATCGATTTA ATGCAAGACA GTTCATGTCC TGGTTGCAAG ATGTGGACGA TAAATTTGAC 
AAGCTAAAGA CCTGTCTCTT AATGAGGCAG CAACACGAAG CTGCAGCTTT AAATGCCGTC 
CAGAGGCTAG AATGGCAGCT CAAACTCCAG GAGCTGGACC CTGCCACGTA TAAGTCTCTC 
AGTATTTATG AAATCCAGGA GTTTTATGTT CCCCTTGTTG ATGTTAATGA TGATTTTGAA 
CTGACTCCTA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105277.1
CDS	152..6286
Translation	MPKSGFTKPVQSENSDSDSSMVERPYGRKSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSGDDESYTDSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTRRERISVDFLSSSTLRSPSLPCLCQRITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSLSIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_030249417.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249417.1

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGAAAAA 
GAAGGTCCAG AGAAGAAGAA AACAAAAAAA GAAGCTGGAA CTAAGAAGTC CACCCCAGTT 
AGCATTCTTT TTGGTTATCC ACTTTCTGAA CGAAAGCAGA TGGCACTCCT AATGCAGATG 
ACAGCAAGGG ACAACAGCCC AGATTCCACA CCAAGTCATC CGTCACAGGC AACGCCAGCC 
CAGAAGAAAA CGCCCAGCTC TTCATCTCGA CAGAAAGATA AAATTAATAA AAGAAATGAG 
CGTGGCGAAA CTCCTTTACA CATGGCCGCT ATTCGAGGAG ACGTGAAGCA AGTCAAAGAG 
TTAATTAGTT TAGGGGCAAA CGTCAATGTG AAGGACTTTG CAGGTTGGAC CCCTTTGCAT 
GAAGCTTGCA ATGTTGGCTA TTACGATGTT GCTAAGATAC TGATAGCAGC TGGCGCAGAC 
GTTAACACAC AAGGACTGGA TGATGACACC CCGCTCCATG ACTCTGCCAG CAGTGGCCAT 
AGAGACATAG TGAAGTTGTT GCTTCGTCAT GGCGGGAATC CATTCCAAGC TAATAAACAT 
GGAGAACGGC CAGTGGATGT GGCAGAGACA GAGGAGTTGG AGTTGCTGCT AAAAAGAGAG 
GTGCCTTTGT CTGGCGATGA TGAAAGTTAT ACAGATTCAG AAGAGGCTCA GTCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCA GAGACAGAGA AAGACTCTTT CGTCTGTGGC 
AGTAAAATCC TCCCAAGCAA AGCCCCGCTG CCTTCTGCAC TGGATGAGTA TGAGTTCAAA 
GATGATGATG AAGAAGAGAT AAGTAAGATG ATTGACGACA GGCACATTCT TAGGAAAGAA 
CAGCGGAGAG AGAATGAATC TGAAGCAGAA AGGAGTGGTC TGTTTGCAAA ACAGGAGAAA 
ACCTTCTATT CTAAGTCATT TAAAACAAAA AAACAGAAGC CGTCAAGGGT TCTGTGCTCC 
AGTACTGAAA GCTCTGATGA AGAGGGTCTT CAGAACAGAA AGATTTCTAC TGCTTGTTCT 
GTTGCTGAAT CATCACATCC TGATATGCAA GCCAAAAAAG AATATGAATA CAAACAGAAA 
GGCAAAGTTA AAAGAAAATT AAAAAATCAG AATAAAAACA AAGAGAACCA AGAGCTGAAA 
CAAGAAAAAG AGGGGAAAGA AAATAGCAGG ATAGCAACCT TGACAGTTAA TACTGCGCTG 
GATTGTTCAG AAAAATACAG AGAGGAGGGG AACTTCAGGA AATCTTTTAG CCCAAAGGAT 
GATACTTCAT TACACTTATT TCATATTTCC ACTGGTAAAT CTCCCAAACA TTCCTGTGGA 
TTAAGTGAAA AACAGACACC ACTGAAGCAA GAACACACGA AAGCATGCAT ATCCCCAGGA 
AGTTCTGAAA TGTCATTACA GCCTGATCTC ACTCGGTATG ATAATGCAGA ACCTGAAAGT 
TCAAATGTCA AATCTTATAA GCACAAGGAA AAAAACAAAC AGCAGAAAGA TTTTCACATA 
GAGTTTGGTG AAAAATCAAA TGCCAAAATT AAAGATGAAG ATCATAATCC AGCATTTGAA 
AACTCAGACT GCACACTGAA GAAGATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAA 
TTAAAACATA AAGAGAAGGA TAAAGAAAAG CACAAAAAGG AAGTTGAAGG TGAAAAGGAA 
AAATATAAAA ATAAAGACAA TGCTAAAGAG CTACAGAGGA GCATCGAATT TGACAGAGAG 
TTTTGGAAAG AAAATTTTTT TAAAAGTGAT GAAACTGAGG ATCTCTTTTT AACTATGGAA 
CATGAGTCCA TAATAGAAAA AAAGACAAAA ATGGAAAAAA ACATGAAAGA TGATAGGTCA 
GCTAAAGAAA AGCATGTCTC AAAAGAAAGG AACTTTAAAG AAGAACGGGA AAAGACTAAA 
AAGGAAAATG AAAAATCTCT TAGGGAAGAA AAGGTAAAAG ATCCGAAAGA AGAAAAAGAC 
AGTGTATCCA CAGAGAAGGA GGCCGAGTGC TGTTCGGTGG GTGTCAGTGT GAGCCAGGAA 
GCTGCAGGGC TGCATTCATC AGAAAAGGAA ATGGATGCTG AGAAGCAAGA AAGACACACA 
AAAGAGAGGG AAAAGTCTGA TCGGCGCTTT CAGACTAAGG AGAAAGAGCT TGACAGGAAC 
GAGAGGAAAT TTTCTGAAAA AGAAAAGAAG ATAAAACATG AACATAAGTC AGAAAGAGAG 
AAGCTGGATT TCAGTGACTG CACTGACAGA GTGAGAGAGA AGGACAAGCT GTACTCACAC 
CAGACAGAAA GGTGCCACAA GGACAGCGAG AAGCTGAAAA ACACTGTCAG AAAGACTGAT 
GACAGAGAGA GGAACAGAGA AAAGGCGGAT AGAAAGCACG ACAGAGAGAA ACCTGAACGA 
GAGAGGTACT TAGCAGAAAA CAAAGAAAAG CACTTAGAGA AAAGAAAGCA ATCTGACAGC 
AGTGAGTATT CCAAATCAGA GAAGGTCAGA ACTAAAGACA GAGACAGGGA AGGAGAGAAG 
AAGGAGAAAC TTAGAGATAA AGAAAGTGTA AATGTAACTA ACTTGAAACA TTTGCAGGAA 
GAGAGAAAGT CCAGTACAGG AGACAGCAGT GGTAAAACCC AGCATGAGAA AGCTCTGTCC 
CTTAAAGAAA GGGCAAGAGA TGAGCCTTTG AAAACTCCAG ATGGAAAAGA GAAAGATAAA 
AAGGATATAG ACAGATACAA AGAGCGAGAC AAGCGTAAAG ATAAAGCCCA GCTAAATACT 
TTAATCAGAC TAAAATCTGA AACAGAGAAG CTTAAACCTA AATTATCACC AGCATCAAAA 
GATACTCGAC CTAAAGAAAA GAGGTTAGTA AATGATGATT TAATGCAGAC AAGTTTTGAA 
CGAATGCTTA GCCTTAAAGA CCTAGAAATA GAACAGTGGC ACAGAAAACA TAAGGAAAAA 
ATTAAGCAAA AGGAAAAAGA ACGATTACGC AATCGTACTT GTTTAGAACT TAAAGTAAAA 
GATAAAGAAA AAACAAAACA TGCACCCACA GAATCCAAAA GTAAAGAACT TACCAGGTCA 
AGAAGTTCAG AGTTGACTGA TGTTTATAGC AAGGAGAAAC AATCTAAAGA TGTTGGCAGT 
AATCGCTCAC AATCTGTTGA CAACAGAAAT GTATTGAATT TAGGAAAGCC ATCCTACATT 
TCAGAGAGTG GCTCCAACCG GTCTCCTAGG TCGGAAGGTG AGAGGCTGGG TCTTAGCTCT 
AGGTCTGTGT CCATGCTTTC TGTTGCTAGT TCAGAAGACT CTTGCCATAC AGTGACAACC 
CCAAGGCCTC TCGTTGAGTA TGACTCTGAC TTTATGTTAG AGGGCTCGGA ATCTCAGTTG 
TCATTTTCTC AGTCACCTTT TTTGCCAAAT GCCAAATCTC CAGCTCTTTA TGAAAGAGAG 
CTGGAGGGTC TCACTGAACT ACCAGACCGA CTTAAACCAC CATATACAAA CAGACTTCCA 
GTGTGCCATC TCAGGTCATC TTCTGTGGAA GATGTTAAAC TAATTACTAA TGAGGTAAGA 
CCTGCTGTAG AAATTAGAAG ATGCAGTATG CCCTCAGTTA TCTGTGAGCC CATAAAACAT 
TTCCAAGTAT CAGAAGAAAG CAGTCAAGGC AACTTAGCTG TTCCCAGAGA GACCTGTCTG 
TCTCCCAAGC CTGAAGTGTC TTCAGCAGTA CCTGAGAGGG GCCTTTCGCA TGTGTCTGAC 
TTACATCCTA ACTGTACAGG CTCTCCAACG AGGTCTGCAA ACAGCAGCTA TATTTCATCT 
GATGTAAATG TAATCAAGAG TGCAACTCTT GTGGGTACAC TGATTGACAG TTCGATGCAT 
TTGGAACCGT CTAATCAGGT TGGAGTGATT CCAAGTAAAT CATGGGAGAC GCCTGTTGAT 
AAACTGGAGT CTTTAAGCAC CAATGACTTC ATCTGTCCAG ATTCTAATAT ATCTGATCAA 
GATTCCTCTG CTCAGAGTTT TTATAATTCA GAAAACAAAA TGTTAAGAGA ACAAAATACT 
GACTGCTTAC CTCCGCACCA GCCTGAACTG CCAGGAAACT CATGTGCTCA GGACCCAGCA 
TCCTTTCTGT CTTCACCTCA GCCCTGCTCT TTTTCCAACC AACCACTGGC AGGTGCTGAC 
TCTGTTCCGA AACCCGTGTC TTTGTCATAT GTTGTCAGTC AAGAGCCAGG TGTTTTACAA 
CAGAAAAGTG CAGCTCAGAT GGCCAGTTCT GTTTTAGATA GTGATAATAA CTTTCCAGAG 
GATGTGGAAA ATACATTTGT CTTAGCAGAC ATTCAGAAGA CAAATTCTTT TTTACCAGCT 
TACTCTGAAA GCACTGTGCA AGAAGCACTC CCAAGCTTTG AGAAGGCCAA TACTTTGCTT 
GTGTTGCCAT CAGAAAAGGA CTTCAATGGG AGTGATACTG CTCAGCCAAA CACACATTAT 
GCATTTAGCA AACTCACATA CAAGTCTTCC AATAGCCAGG AAATTGAGAA GGGCCCAGAT 
GATACTCAAA TCATTTCACA TGAAAAGGAA AACAAATTAC AAAGTTTGGT CTTAACTCAG 
TTGAGTAAAT GTGATTCTGA TTTGTATGAG ATGAATGCAG GGCTGCCAAA AGGAAACCTG 
AATGAACAGG ATAACCCAAA GCATGGTCTT GACAGCGAAA AGTACTCGCT TTCCACTGAA 
GATGAAGAAT CTCAGCAGAG CACCTTATCA AGTGTGGAAA ACCATTCACA GCAGTCGGCC 
CAACCAGAGA TGCATAAATA TGACCAGCTT AGTAAAGTAG AACTAGAAGA AAATGCCGAA 
GACGATAAAA CTGAAAACCA GACCCCTCAA AGAGTGACTA GAAACAAAGC AAGCACAGTG 
GTCAATCCAA GCAAGCCCAT GCTTGCTGGC TGTGCCCTTG TAGCAGAGAA AGATAACGAG 
TCCTCCCCTA GGGGACGAAT CAGACTCACT GAAGAGGATG ATCCTCAGAT CCACCATCCT 
CGGAAGAGGA AAGTGTCACG GGTTCCTCAG CCTGTGCAAG TGAGCCCCTC TTTACTGCAA 
GCAAAGGAGA AAACTCAGCA GTCTCTAGCA GCCATTGTAG ATTCTCTGAA GTTAGATGAG 
ATTGAGCCAT ACAGTTCTGA GAGAGCAAAT CCATATTTTG AATATTTGCA CATAAGAAAA 
AAAATTGAAG AAAAACGCAA GTTGCTATGT AGCGTTATCC CTCAAGCTCC TCAGTATTAT 
GATGAATACG TAACGTTTAA TGGATCCTAT CTCCTGGATG GAAACCCCTT AAGCAAGATT 
TGTATTCCTA CAAGAAGGGA GAGAATCTCG GTTGATTTTC TCAGCTCATC AACCTTAAGG 
TCACCTTCAC TGCCATGCTT GTGCCAAAGG ATCACACCAC CACCTTCGCT GTCAGATCCA 
CTTAAAGAGC TCTTTCGGCA ACAGGAAGTA GTAAGGATGA AACTGCGTTT GCAACATAGT 
ATTGAAAGGG AAAAACTTAT TGTATCCAAT GAACAAGAAG TTCTGCGAGT TCATTACAGA 
GCTGCAAGAA CACTGGCCAA TCAAACACTG CCATTTAGTG CTTGTACTGT CTTACTGGAT 
GCAGAAGTGT ACAATGTGCC CTTGGACTCA CAGTCTGATG ACAGCAAAAC TTCTGTGAGA 
GATCGATTTA ATGCAAGACA GTTCATGTCC TGGTTGCAAG ATGTGGACGA TAAATTTGAC 
AAGCTAAAGA CCTGTCTCTT AATGAGGCAG CAACACGAAG CTGCAGCTTT AAATGCCGTC 
CAGAGGCTAG AATGGCAGCT CAAACTCCAG GAGCTGGACC CTGCCACGTA TAAGTCTCTC 
AGTATTTATG AAATCCAGGA GTTTTATGTT CCCCTTGTTG ATGTTAATGA TGATTTTGAA 
CTGACTCCTA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( XM_006523399.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523462.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X9, mRNA.

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CloneID	OMu52957
Clone ID Related Accession (Same CDS sequence)	XM_006523399.4 , XM_006523399.3
Accession Version	XM_006523399.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523399.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGAAAAA 
GAAGGTCCAG AGAAGAAGAA AACAAAAAAA GAAGCTGGAA CTAAGAAGTC CACCCCAGTT 
AGCATTCTTT TTGGTTATCC ACTTTCTGAA CGAAAGCAGA TGGCACTCCT AATGCAGATG 
ACAGCAAGGG ACAACAGCCC AGATTCCACA CCAAGTCATC CGTCACAGGC AACGCCAGCC 
CAGAAGAAAA CGCCCAGCTC TTCATCTCGA CAGAAAGATA AAATTAATAA AAGAAATGAG 
CGTGGCGAAA CTCCTTTACA CATGGCCGCT ATTCGAGGAG ACGTGAAGCA AGTCAAAGAG 
TTAATTAGTT TAGGGGCAAA CGTCAATGTG AAGGACTTTG CAGGTTGGAC CCCTTTGCAT 
GAAGCTTGCA ATGTTGGCTA TTACGATGTT GCTAAGATAC TGATAGCAGC TGGCGCAGAC 
GTTAACACAC AAGGACTGGA TGATGACACC CCGCTCCATG ACTCTGCCAG CAGTGGCCAT 
AGAGACATAG TGAAGTTGTT GCTTCGTCAT GGCGGGAATC CATTCCAAGC TAATAAACAT 
GGAGAACGGC CAGTGGATGT GGCAGAGACA GAGGAGTTGG AGTTGCTGCT AAAAAGAGAG 
GTGCCTTTGT CTGGCGATGA TGAAAGTTAT ACAGATTCAG AAGAGGCTCA GTCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCA GAGACAGAGA AAGACTCTTT CGTCTGTGGC 
AGTAAAATCC TCCCAAGCAA AGCCCCGCTG CCTTCTGCAC TGGATGAGTA TGAGTTCAAA 
GATGATGATG AAGAAGAGAT AAGTAAGATG ATTGACGACA GGCACATTCT TAGGAAAGAA 
CAGCGGAGAG AGAATGAATC TGAAGCAGAA AGGAGTGGTC TGTTTGCAAA ACAGGAGAAA 
ACCTTCTATT CTAAGTCATT TAAAACAAAA AAACAGAAGC CGTCAAGGGT TCTGTGCTCC 
AGTACTGAAA GCTCTGATGA AGAGGGTCTT CAGAACAGAA AGATTTCTAC TGCTTGTTCT 
GTTGCTGAAT CATCACATCC TGATATGCAA GCCAAAAAAG AATATGAATA CAAACAGAAA 
GGCAAAGTTA AAAGAAAATT AAAAAATCAG AATAAAAACA AAGAGAACCA AGAGCTGAAA 
CAAGAAAAAG AGGGGAAAGA AAATAGCAGG ATAGCAACCT TGACAGTTAA TACTGCGCTG 
GATTGTTCAG AAAAATACAG AGAGGAGGGG AACTTCAGGA AATCTTTTAG CCCAAAGGAT 
GATACTTCAT TACACTTATT TCATATTTCC ACTGGTAAAT CTCCCAAACA TTCCTGTGGA 
TTAAGTGAAA AACAGACACC ACTGAAGCAA GAACACACGA AAGCATGCAT ATCCCCAGGA 
AGTTCTGAAA TGTCATTACA GCCTGATCTC ACTCGGTATG ATAATGCAGA ACCTGAAAGT 
TCAAATGTCA AATCTTATAA GCACAAGGAA AAAAACAAAC AGCAGAAAGA TTTTCACATA 
GAGTTTGGTG AAAAATCAAA TGCCAAAATT AAAGATGAAG ATCATAATCC AGCATTTGAA 
AACTCAGACT GCACACTGAA GAAGATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAA 
TTAAAACATA AAGAGAAGGA TAAAGAAAAG CACAAAAAGG AAGTTGAAGG TGAAAAGGAA 
AAATATAAAA ATAAAGACAA TGCTAAAGAG CTACAGAGGA GCATCGAATT TGACAGAGAG 
TTTTGGAAAG AAAATTTTTT TAAAAGTGAT GAAACTGAGG ATCTCTTTTT AACTATGGAA 
CATGAGTCCA TAATAGAAAA AAAGACAAAA ATGGAAAAAA ACATGAAAGA TGATAGGTCA 
GCTAAAGAAA AGCATGTCTC AAAAGAAAGG AACTTTAAAG AAGAACGGGA AAAGACTAAA 
AAGGAAAATG AAAAATCTCT TAGGGAAGAA AAGGTAAAAG ATCCGAAAGA AGAAAAAGAC 
AGTGTATCCA CAGAGAAGGA GGCCGAGTGC TGTTCGGTGG GTGTCAGTGT GAGCCAGGAA 
GCTGCAGGGC TGCATTCATC AGAAAAGGAA ATGGATGCTG AGAAGCAAGA AAGACACACA 
AAAGAGAGGG AAAAGTCTGA TCGGCGCTTT CAGACTAAGG AGAAAGAGCT TGACAGGAAC 
GAGAGGAAAT TTTCTGAAAA AGAAAAGAAG ATAAAACATG AACATAAGTC AGAAAGAGAG 
AAGCTGGATT TCAGTGACTG CACTGACAGA GTGAGAGAGA AGGACAAGCT GTACTCACAC 
CAGACAGAAA GGTGCCACAA GGACAGCGAG AAGCTGAAAA ACACTGTCAG AAAGACTGAT 
GACAGAGAGA GGAACAGAGA AAAGGCGGAT AGAAAGCACG ACAGAGAGAA ACCTGAACGA 
GAGAGGTACT TAGCAGAAAA CAAAGAAAAG CACTTAGAGA AAAGAAAGCA ATCTGACAGC 
AGTGAGTATT CCAAATCAGA GAAGGTCAGA ACTAAAGACA GAGACAGGGA AGGAGAGAAG 
AAGGAGAAAC TTAGAGATAA AGAAAGTGTA AATGTAACTA ACTTGAAACA TTTGCAGGAA 
GAGAGAAAGT CCAGTACAGG AGACAGCAGT GGTAAAACCC AGCATGAGAA AGCTCTGTCC 
CTTAAAGAAA GGGCAAGAGA TGAGCCTTTG AAAACTCCAG ATGGAAAAGA GAAAGATAAA 
AAGGATATAG ACAGATACAA AGAGCGAGAC AAGCGTAAAG ATAAAGCCCA GCTAAATACT 
TTAATCAGAC TAAAATCTGA AACAGAGAAG CTTAAACCTA AATTATCACC AGCATCAAAA 
GATACTCGAC CTAAAGAAAA GAGGTTAGTA AATGATGATT TAATGCAGAC AAGTTTTGAA 
CGAATGCTTA GCCTTAAAGA CCTAGAAATA GAACAGTGGC ACAGAAAACA TAAGGAAAAA 
ATTAAGCAAA AGGAAAAAGA ACGATTACGC AATCGTACTT GTTTAGAACT TAAAGTAAAA 
GATAAAGAAA AAACAAAACA TGCACCCACA GAATCCAAAA GTAAAGAACT TACCAGGTCA 
AGAAGTTCAG AGTTGACTGA TGTTTATAGC AAGGAGAAAC AATCTAAAGA TGTTGGCAGT 
AATCGCTCAC AATCTGTTGA CAACAGAAAT GTATTGAATT TAGGAAAGCC ATCCTACATT 
TCAGAGAGTG GCTCCAACCG GTCTCCTAGG TCGGAAGGTG AGAGGCTGGG TCTTAGCTCT 
AGGTCTGTGT CCATGCTTTC TGTTGCTAGT TCAGAAGACT CTTGCCATAC AGTGACAACC 
CCAAGGCCTC TCGTTGAGTA TGACTCTGAC TTTATGTTAG AGGGCTCGGA ATCTCAGTTG 
TCATTTTCTC AGTCACCTTT TTTGCCAAAT GCCAAATCTC CAGCTCTTTA TGAAAGAGAG 
CTGGAGGGTC TCACTGAACT ACCAGACCGA CTTAAACCAC CATATACAAA CAGACTTCCA 
GTGTGCCATC TCAGGTCATC TTCTGTGGAA GATGTTAAAC TAATTACTAA TGAGGTAAGA 
CCTGCTGTAG AAATTAGAAG ATGCAGTATG CCCTCAGTTA TCTGTGAGCC CATAAAACAT 
TTCCAAGTAT CAGAAGAAAG CAGTCAAGGC AACTTAGCTG TTCCCAGAGA GACCTGTCTG 
TCTCCCAAGC CTGAAGTGTC TTCAGCAGTA CCTGAGAGGG GCCTTTCGCA TGTGTCTGAC 
TTACATCCTA ACTGTACAGG CTCTCCAACG AGGTCTGCAA ACAGCAGCTA TATTTCATCT 
GATGTAAATG TAATCAAGAG TGCAACTCTT GTGGGTACAC TGATTGACAG TTCGATGCAT 
TTGGAACCGT CTAATCAGGT TGGAGTGATT CCAAGTAAAT CATGGGAGAC GCCTGTTGAT 
AAACTGGAGT CTTTAAGCAC CAATGACTTC ATCTGTCCAG ATTCTAATAT ATCTGATCAA 
GATTCCTCTG CTCAGAGTTT TTATAATTCA GAAAACAAAA TGTTAAGAGA ACAAAATACT 
GACTGCTTAC CTCCGCACCA GCCTGAACTG CCAGGAAACT CATGTGCTCA GGACCCAGCA 
TCCTTTCTGT CTTCACCTCA GCCCTGCTCT TTTTCCAACC AACCACTGGC AGGTGCTGAC 
TCTGTTCCGA AACCCGTGTC TTTGTCATAT GTTGTCAGTC AAGAGCCAGG TGTTTTACAA 
CAGAAAAGTG CAGCTCAGAT GGCCAGTTCT GTTTTAGATA GTGATAATAA CTTTCCAGAG 
GATGTGGAAA ATACATTTGT CTTAGCAGAC ATTCAGAAGA CAAATTCTTT TTTACCAGCT 
TACTCTGAAA GCACTGTGCA AGAAGCACTC CCAAGCTTTG AGAAGGCCAA TACTTTGCTT 
GTGTTGCCAT CAGAAAAGGA CTTCAATGGG AGTGATACTG CTCAGCCAAA CACACATTAT 
GCATTTAGCA AACTCACATA CAAGTCTTCC AATAGCCAGG AAATTGAGAA GGGCCCAGAT 
GATACTCAAA TCATTTCACA TGAAAAGGAA AACAAATTAC AAAGTTTGGT CTTAACTCAG 
TTGAGTAAAT GTGATTCTGA TTTGTATGAG ATGAATGCAG GGCTGCCAAA AGGAAACCTG 
AATGAACAGG ATAACCCAAA GCATGGTCTT GACAGCGAAA AGTACTCGCT TTCCACTGAA 
GATGAAGAAT CTCAGCAGAG CACCTTATCA AGTGTGGAAA ACCATTCACA GCAGTCGGCC 
CAACCAGAGA TGCATAAATA TGACCAGCTT AGTAAAGTAG AACTAGAAGA AAATGCCGAA 
GACGATAAAA CTGAAAACCA GACCCCTCAA AGAGTGACTA GAAACAAAGC AAGCACAGTG 
GTCAATCCAA GCAAGCCCAT GCTTGCTGGC TGTGCCCTTG TAGCAGAGAA AGATAACGAG 
TCCTCCCCTA GGGGACGAAT CAGACTCACT GAAGAGGATG ATCCTCAGAT CCACCATCCT 
CGGAAGAGGA AAGTGTCACG GGTTCCTCAG CCTGTGCAAG TGAGCCCCTC TTTACTGCAA 
GCAAAGGAGA AAACTCAGCA GTCTCTAGCA GCCATTGTAG ATTCTCTGAA GTTAGATGAG 
ATTGAGCCAT ACAGTTCTGA GAGAGCAAAT CCATATTTTG AATATTTGCA CATAAGAAAA 
AAAATTGAAG AAAAACGCAA GTTGCTATGT AGCGTTATCC CTCAAGCTCC TCAGTATTAT 
GATGAATACG TAACGTTTAA TGGATCCTAT CTCCTGGATG GAAACCCCTT AAGCAAGATT 
TGTATTCCTA CAATCACACC ACCACCTTCG CTGTCAGATC CACTTAAAGA GCTCTTTCGG 
CAACAGGAAG TAGTAAGGAT GAAACTGCGT TTGCAACATA GTATTGAAAG GGAAAAACTT 
ATTGTATCCA ATGAACAAGA AGTTCTGCGA GTTCATTACA GAGCTGCAAG AACACTGGCC 
AATCAAACAC TGCCATTTAG TGCTTGTACT GTCTTACTGG ATGCAGAAGT GTACAATGTG 
CCCTTGGACT CACAGTCTGA TGACAGCAAA ACTTCTGTGA GAGATCGATT TAATGCAAGA 
CAGTTCATGT CCTGGTTGCA AGATGTGGAC GATAAATTTG ACAAGCTAAA GACCTGTCTC 
TTAATGAGGC AGCAACACGA AGCTGCAGCT TTAAATGCCG TCCAGAGGCT AGAATGGCAG 
CTCAAACTCC AGGAGCTGGA CCCTGCCACG TATAAGTCTC TCAGTATTTA TGAAATCCAG 
GAGTTTTATG TTCCCCTTGT TGATGTTAAT GATGATTTTG AACTGACTCC TATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523462.1
CDS	269..6325
Translation	MPKSGFTKPVQSENSDSDSSMVERPYGRKSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSGDDESYTDSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSLSIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_006523399.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523399.4

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGAAAAA 
GAAGGTCCAG AGAAGAAGAA AACAAAAAAA GAAGCTGGAA CTAAGAAGTC CACCCCAGTT 
AGCATTCTTT TTGGTTATCC ACTTTCTGAA CGAAAGCAGA TGGCACTCCT AATGCAGATG 
ACAGCAAGGG ACAACAGCCC AGATTCCACA CCAAGTCATC CGTCACAGGC AACGCCAGCC 
CAGAAGAAAA CGCCCAGCTC TTCATCTCGA CAGAAAGATA AAATTAATAA AAGAAATGAG 
CGTGGCGAAA CTCCTTTACA CATGGCCGCT ATTCGAGGAG ACGTGAAGCA AGTCAAAGAG 
TTAATTAGTT TAGGGGCAAA CGTCAATGTG AAGGACTTTG CAGGTTGGAC CCCTTTGCAT 
GAAGCTTGCA ATGTTGGCTA TTACGATGTT GCTAAGATAC TGATAGCAGC TGGCGCAGAC 
GTTAACACAC AAGGACTGGA TGATGACACC CCGCTCCATG ACTCTGCCAG CAGTGGCCAT 
AGAGACATAG TGAAGTTGTT GCTTCGTCAT GGCGGGAATC CATTCCAAGC TAATAAACAT 
GGAGAACGGC CAGTGGATGT GGCAGAGACA GAGGAGTTGG AGTTGCTGCT AAAAAGAGAG 
GTGCCTTTGT CTGGCGATGA TGAAAGTTAT ACAGATTCAG AAGAGGCTCA GTCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCA GAGACAGAGA AAGACTCTTT CGTCTGTGGC 
AGTAAAATCC TCCCAAGCAA AGCCCCGCTG CCTTCTGCAC TGGATGAGTA TGAGTTCAAA 
GATGATGATG AAGAAGAGAT AAGTAAGATG ATTGACGACA GGCACATTCT TAGGAAAGAA 
CAGCGGAGAG AGAATGAATC TGAAGCAGAA AGGAGTGGTC TGTTTGCAAA ACAGGAGAAA 
ACCTTCTATT CTAAGTCATT TAAAACAAAA AAACAGAAGC CGTCAAGGGT TCTGTGCTCC 
AGTACTGAAA GCTCTGATGA AGAGGGTCTT CAGAACAGAA AGATTTCTAC TGCTTGTTCT 
GTTGCTGAAT CATCACATCC TGATATGCAA GCCAAAAAAG AATATGAATA CAAACAGAAA 
GGCAAAGTTA AAAGAAAATT AAAAAATCAG AATAAAAACA AAGAGAACCA AGAGCTGAAA 
CAAGAAAAAG AGGGGAAAGA AAATAGCAGG ATAGCAACCT TGACAGTTAA TACTGCGCTG 
GATTGTTCAG AAAAATACAG AGAGGAGGGG AACTTCAGGA AATCTTTTAG CCCAAAGGAT 
GATACTTCAT TACACTTATT TCATATTTCC ACTGGTAAAT CTCCCAAACA TTCCTGTGGA 
TTAAGTGAAA AACAGACACC ACTGAAGCAA GAACACACGA AAGCATGCAT ATCCCCAGGA 
AGTTCTGAAA TGTCATTACA GCCTGATCTC ACTCGGTATG ATAATGCAGA ACCTGAAAGT 
TCAAATGTCA AATCTTATAA GCACAAGGAA AAAAACAAAC AGCAGAAAGA TTTTCACATA 
GAGTTTGGTG AAAAATCAAA TGCCAAAATT AAAGATGAAG ATCATAATCC AGCATTTGAA 
AACTCAGACT GCACACTGAA GAAGATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAA 
TTAAAACATA AAGAGAAGGA TAAAGAAAAG CACAAAAAGG AAGTTGAAGG TGAAAAGGAA 
AAATATAAAA ATAAAGACAA TGCTAAAGAG CTACAGAGGA GCATCGAATT TGACAGAGAG 
TTTTGGAAAG AAAATTTTTT TAAAAGTGAT GAAACTGAGG ATCTCTTTTT AACTATGGAA 
CATGAGTCCA TAATAGAAAA AAAGACAAAA ATGGAAAAAA ACATGAAAGA TGATAGGTCA 
GCTAAAGAAA AGCATGTCTC AAAAGAAAGG AACTTTAAAG AAGAACGGGA AAAGACTAAA 
AAGGAAAATG AAAAATCTCT TAGGGAAGAA AAGGTAAAAG ATCCGAAAGA AGAAAAAGAC 
AGTGTATCCA CAGAGAAGGA GGCCGAGTGC TGTTCGGTGG GTGTCAGTGT GAGCCAGGAA 
GCTGCAGGGC TGCATTCATC AGAAAAGGAA ATGGATGCTG AGAAGCAAGA AAGACACACA 
AAAGAGAGGG AAAAGTCTGA TCGGCGCTTT CAGACTAAGG AGAAAGAGCT TGACAGGAAC 
GAGAGGAAAT TTTCTGAAAA AGAAAAGAAG ATAAAACATG AACATAAGTC AGAAAGAGAG 
AAGCTGGATT TCAGTGACTG CACTGACAGA GTGAGAGAGA AGGACAAGCT GTACTCACAC 
CAGACAGAAA GGTGCCACAA GGACAGCGAG AAGCTGAAAA ACACTGTCAG AAAGACTGAT 
GACAGAGAGA GGAACAGAGA AAAGGCGGAT AGAAAGCACG ACAGAGAGAA ACCTGAACGA 
GAGAGGTACT TAGCAGAAAA CAAAGAAAAG CACTTAGAGA AAAGAAAGCA ATCTGACAGC 
AGTGAGTATT CCAAATCAGA GAAGGTCAGA ACTAAAGACA GAGACAGGGA AGGAGAGAAG 
AAGGAGAAAC TTAGAGATAA AGAAAGTGTA AATGTAACTA ACTTGAAACA TTTGCAGGAA 
GAGAGAAAGT CCAGTACAGG AGACAGCAGT GGTAAAACCC AGCATGAGAA AGCTCTGTCC 
CTTAAAGAAA GGGCAAGAGA TGAGCCTTTG AAAACTCCAG ATGGAAAAGA GAAAGATAAA 
AAGGATATAG ACAGATACAA AGAGCGAGAC AAGCGTAAAG ATAAAGCCCA GCTAAATACT 
TTAATCAGAC TAAAATCTGA AACAGAGAAG CTTAAACCTA AATTATCACC AGCATCAAAA 
GATACTCGAC CTAAAGAAAA GAGGTTAGTA AATGATGATT TAATGCAGAC AAGTTTTGAA 
CGAATGCTTA GCCTTAAAGA CCTAGAAATA GAACAGTGGC ACAGAAAACA TAAGGAAAAA 
ATTAAGCAAA AGGAAAAAGA ACGATTACGC AATCGTACTT GTTTAGAACT TAAAGTAAAA 
GATAAAGAAA AAACAAAACA TGCACCCACA GAATCCAAAA GTAAAGAACT TACCAGGTCA 
AGAAGTTCAG AGTTGACTGA TGTTTATAGC AAGGAGAAAC AATCTAAAGA TGTTGGCAGT 
AATCGCTCAC AATCTGTTGA CAACAGAAAT GTATTGAATT TAGGAAAGCC ATCCTACATT 
TCAGAGAGTG GCTCCAACCG GTCTCCTAGG TCGGAAGGTG AGAGGCTGGG TCTTAGCTCT 
AGGTCTGTGT CCATGCTTTC TGTTGCTAGT TCAGAAGACT CTTGCCATAC AGTGACAACC 
CCAAGGCCTC TCGTTGAGTA TGACTCTGAC TTTATGTTAG AGGGCTCGGA ATCTCAGTTG 
TCATTTTCTC AGTCACCTTT TTTGCCAAAT GCCAAATCTC CAGCTCTTTA TGAAAGAGAG 
CTGGAGGGTC TCACTGAACT ACCAGACCGA CTTAAACCAC CATATACAAA CAGACTTCCA 
GTGTGCCATC TCAGGTCATC TTCTGTGGAA GATGTTAAAC TAATTACTAA TGAGGTAAGA 
CCTGCTGTAG AAATTAGAAG ATGCAGTATG CCCTCAGTTA TCTGTGAGCC CATAAAACAT 
TTCCAAGTAT CAGAAGAAAG CAGTCAAGGC AACTTAGCTG TTCCCAGAGA GACCTGTCTG 
TCTCCCAAGC CTGAAGTGTC TTCAGCAGTA CCTGAGAGGG GCCTTTCGCA TGTGTCTGAC 
TTACATCCTA ACTGTACAGG CTCTCCAACG AGGTCTGCAA ACAGCAGCTA TATTTCATCT 
GATGTAAATG TAATCAAGAG TGCAACTCTT GTGGGTACAC TGATTGACAG TTCGATGCAT 
TTGGAACCGT CTAATCAGGT TGGAGTGATT CCAAGTAAAT CATGGGAGAC GCCTGTTGAT 
AAACTGGAGT CTTTAAGCAC CAATGACTTC ATCTGTCCAG ATTCTAATAT ATCTGATCAA 
GATTCCTCTG CTCAGAGTTT TTATAATTCA GAAAACAAAA TGTTAAGAGA ACAAAATACT 
GACTGCTTAC CTCCGCACCA GCCTGAACTG CCAGGAAACT CATGTGCTCA GGACCCAGCA 
TCCTTTCTGT CTTCACCTCA GCCCTGCTCT TTTTCCAACC AACCACTGGC AGGTGCTGAC 
TCTGTTCCGA AACCCGTGTC TTTGTCATAT GTTGTCAGTC AAGAGCCAGG TGTTTTACAA 
CAGAAAAGTG CAGCTCAGAT GGCCAGTTCT GTTTTAGATA GTGATAATAA CTTTCCAGAG 
GATGTGGAAA ATACATTTGT CTTAGCAGAC ATTCAGAAGA CAAATTCTTT TTTACCAGCT 
TACTCTGAAA GCACTGTGCA AGAAGCACTC CCAAGCTTTG AGAAGGCCAA TACTTTGCTT 
GTGTTGCCAT CAGAAAAGGA CTTCAATGGG AGTGATACTG CTCAGCCAAA CACACATTAT 
GCATTTAGCA AACTCACATA CAAGTCTTCC AATAGCCAGG AAATTGAGAA GGGCCCAGAT 
GATACTCAAA TCATTTCACA TGAAAAGGAA AACAAATTAC AAAGTTTGGT CTTAACTCAG 
TTGAGTAAAT GTGATTCTGA TTTGTATGAG ATGAATGCAG GGCTGCCAAA AGGAAACCTG 
AATGAACAGG ATAACCCAAA GCATGGTCTT GACAGCGAAA AGTACTCGCT TTCCACTGAA 
GATGAAGAAT CTCAGCAGAG CACCTTATCA AGTGTGGAAA ACCATTCACA GCAGTCGGCC 
CAACCAGAGA TGCATAAATA TGACCAGCTT AGTAAAGTAG AACTAGAAGA AAATGCCGAA 
GACGATAAAA CTGAAAACCA GACCCCTCAA AGAGTGACTA GAAACAAAGC AAGCACAGTG 
GTCAATCCAA GCAAGCCCAT GCTTGCTGGC TGTGCCCTTG TAGCAGAGAA AGATAACGAG 
TCCTCCCCTA GGGGACGAAT CAGACTCACT GAAGAGGATG ATCCTCAGAT CCACCATCCT 
CGGAAGAGGA AAGTGTCACG GGTTCCTCAG CCTGTGCAAG TGAGCCCCTC TTTACTGCAA 
GCAAAGGAGA AAACTCAGCA GTCTCTAGCA GCCATTGTAG ATTCTCTGAA GTTAGATGAG 
ATTGAGCCAT ACAGTTCTGA GAGAGCAAAT CCATATTTTG AATATTTGCA CATAAGAAAA 
AAAATTGAAG AAAAACGCAA GTTGCTATGT AGCGTTATCC CTCAAGCTCC TCAGTATTAT 
GATGAATACG TAACGTTTAA TGGATCCTAT CTCCTGGATG GAAACCCCTT AAGCAAGATT 
TGTATTCCTA CAATCACACC ACCACCTTCG CTGTCAGATC CACTTAAAGA GCTCTTTCGG 
CAACAGGAAG TAGTAAGGAT GAAACTGCGT TTGCAACATA GTATTGAAAG GGAAAAACTT 
ATTGTATCCA ATGAACAAGA AGTTCTGCGA GTTCATTACA GAGCTGCAAG AACACTGGCC 
AATCAAACAC TGCCATTTAG TGCTTGTACT GTCTTACTGG ATGCAGAAGT GTACAATGTG 
CCCTTGGACT CACAGTCTGA TGACAGCAAA ACTTCTGTGA GAGATCGATT TAATGCAAGA 
CAGTTCATGT CCTGGTTGCA AGATGTGGAC GATAAATTTG ACAAGCTAAA GACCTGTCTC 
TTAATGAGGC AGCAACACGA AGCTGCAGCT TTAAATGCCG TCCAGAGGCT AGAATGGCAG 
CTCAAACTCC AGGAGCTGGA CCCTGCCACG TATAAGTCTC TCAGTATTTA TGAAATCCAG 
GAGTTTTATG TTCCCCTTGT TGATGTTAAT GATGATTTTG AACTGACTCC TATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( XM_030249419.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105279.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X8, mRNA.

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CloneID	OMu87054
Clone ID Related Accession (Same CDS sequence)	XM_030249419.1
Accession Version	XM_030249419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AATCACACCA CCACCTTCGC TGTCAGATCC ACTTAAAGAG 
CTCTTTCGGC AACAGGAAGT AGTAAGGATG AAACTGCGTT TGCAACATAG TATTGAAAGG 
GAAAAACTTA TTGTATCCAA TGAACAAGAA GTTCTGCGAG TTCATTACAG AGCTGCAAGA 
ACACTGGCCA ATCAAACACT GCCATTTAGT GCTTGTACTG TCTTACTGGA TGCAGAAGTG 
TACAATGTGC CCTTGGACTC ACAGTCTGAT GACAGCAAAA CTTCTGTGAG AGATCGATTT 
AATGCAAGAC AGTTCATGTC CTGGTTGCAA GATGTGGACG ATAAATTTGA CAAGCTAAAG 
AAAGAAAATT TGATGAGCTC GTGTGGCAGA TTACAAAAAT TAAAAAAGAT TAAATGTTCA 
AGCAAGCAAG AGAAAAGGAA ATCTGTAGAG CATTTGGTTC TGATGGTCCC GCCAGGGACC 
AGTCGAGGAA GTAGTACTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105279.1
CDS	258..6341
Translation	MPKSGFTKPVQSENSDSDSSMVERPYGRKSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSGDDESYTDSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKKENLMSSCGRLQKLKKIKCSSKQEKRKSVEHLVLMVPPGTSRGSSTD

Target ORF information:

RefSeq Version	XM_030249419.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249419.1

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AATCACACCA CCACCTTCGC TGTCAGATCC ACTTAAAGAG 
CTCTTTCGGC AACAGGAAGT AGTAAGGATG AAACTGCGTT TGCAACATAG TATTGAAAGG 
GAAAAACTTA TTGTATCCAA TGAACAAGAA GTTCTGCGAG TTCATTACAG AGCTGCAAGA 
ACACTGGCCA ATCAAACACT GCCATTTAGT GCTTGTACTG TCTTACTGGA TGCAGAAGTG 
TACAATGTGC CCTTGGACTC ACAGTCTGAT GACAGCAAAA CTTCTGTGAG AGATCGATTT 
AATGCAAGAC AGTTCATGTC CTGGTTGCAA GATGTGGACG ATAAATTTGA CAAGCTAAAG 
AAAGAAAATT TGATGAGCTC GTGTGGCAGA TTACAAAAAT TAAAAAAGAT TAAATGTTCA 
AGCAAGCAAG AGAAAAGGAA ATCTGTAGAG CATTTGGTTC TGATGGTCCC GCCAGGGACC 
AGTCGAGGAA GTAGTACTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( NM_001025572.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001020743.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), mRNA.

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CloneID	OMu12207
Clone ID Related Accession (Same CDS sequence)	NM_001025572.1
Accession Version	NM_001025572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC119957.9, CF907970.1, BC023046.1, CF553222.1, AC121299.16 and AK122392.1. On Aug 6, 2005 this sequence version replaced XM_619483.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AATCACACCA CCACCTTCGC TGTCAGATCC ACTTAAAGAG 
CTCTTTCGGC AACAGGAAGT AGTAAGGATG AAACTGCGTT TGCAACATAG TATTGAAAGG 
GAAAAACTTA TTGTATCCAA TGAACAAGAA GTTCTGCGAG TTCATTACAG AGCTGCAAGA 
ACACTGGCCA ATCAAACACT GCCATTTAGT GCTTGTACTG TCTTACTGGA TGCAGAAGTG 
TACAATGTGC CCTTGGACTC ACAGTCTGAT GACAGCAAAA CTTCTGTGAG AGATCGATTT 
AATGCAAGAC AGTTCATGTC CTGGTTGCAA GATGTGGACG ATAAATTTGA CAAGCTAAAG 
ACCTGTCTCT TAATGAGGCA GCAACACGAA GCTGCAGCTT TAAATGCCGT CCAGAGGCTA 
GAATGGCAGC TCAAACTCCA GGAGCTGGAC CCTGCCACGT ATAAGTCTCT CAGTATTTAT 
GAAATCCAGG AGTTTTATGT TCCCCTTGTT GATGTTAATG ATGATTTTGA ACTGACTCCT 
ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020743.1
CDS	215..6340
Translation	MPKSGFTKPVQSENSDSDSSMVERPYGRKSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSGDDESYTDSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSLSIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	NM_001025572.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025572.1

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AATCACACCA CCACCTTCGC TGTCAGATCC ACTTAAAGAG 
CTCTTTCGGC AACAGGAAGT AGTAAGGATG AAACTGCGTT TGCAACATAG TATTGAAAGG 
GAAAAACTTA TTGTATCCAA TGAACAAGAA GTTCTGCGAG TTCATTACAG AGCTGCAAGA 
ACACTGGCCA ATCAAACACT GCCATTTAGT GCTTGTACTG TCTTACTGGA TGCAGAAGTG 
TACAATGTGC CCTTGGACTC ACAGTCTGAT GACAGCAAAA CTTCTGTGAG AGATCGATTT 
AATGCAAGAC AGTTCATGTC CTGGTTGCAA GATGTGGACG ATAAATTTGA CAAGCTAAAG 
ACCTGTCTCT TAATGAGGCA GCAACACGAA GCTGCAGCTT TAAATGCCGT CCAGAGGCTA 
GAATGGCAGC TCAAACTCCA GGAGCTGGAC CCTGCCACGT ATAAGTCTCT CAGTATTTAT 
GAAATCCAGG AGTTTTATGT TCCCCTTGTT GATGTTAATG ATGATTTTGA ACTGACTCCT 
ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( XM_006523395.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523458.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X3, mRNA.

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CloneID	OMu52953
Clone ID Related Accession (Same CDS sequence)	XM_006523395.3 , XM_006523395.4 , XM_006523394.3 , XM_006523393.4 , XM_006523394.4 , XM_006523393.3
Accession Version	XM_006523395.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523395.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AAGAAGGGAG AGAATCTCGG TTGATTTTCT CAGCTCATCA 
ACCTTAAGGT CACCTTCACT GCCATGCTTG TGCCAAAGGA TCACACCACC ACCTTCGCTG 
TCAGATCCAC TTAAAGAGCT CTTTCGGCAA CAGGAAGTAG TAAGGATGAA ACTGCGTTTG 
CAACATAGTA TTGAAAGGGA AAAACTTATT GTATCCAATG AACAAGAAGT TCTGCGAGTT 
CATTACAGAG CTGCAAGAAC ACTGGCCAAT CAAACACTGC CATTTAGTGC TTGTACTGTC 
TTACTGGATG CAGAAGTGTA CAATGTGCCC TTGGACTCAC AGTCTGATGA CAGCAAAACT 
TCTGTGAGAG ATCGATTTAA TGCAAGACAG TTCATGTCCT GGTTGCAAGA TGTGGACGAT 
AAATTTGACA AGCTAAAGAC CTGTCTCTTA ATGAGGCAGC AACACGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGCTAGA ATGGCAGCTC AAACTCCAGG AGCTGGACCC TGCCACGTAT 
AAGTCTCTCA GTATTTATGA AATCCAGGAG TTTTATGTTC CCCTTGTTGA TGTTAATGAT 
GATTTTGAAC TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523458.1
CDS	168..6371
Translation	MPKSGFTKPVQSENSDSDSSMVERPYGRKSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSGDDESYTDSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTRRERISVDFLSSSTLRSPSLPCLCQRITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSLSIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_006523395.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523395.3

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AAGAAGGGAG AGAATCTCGG TTGATTTTCT CAGCTCATCA 
ACCTTAAGGT CACCTTCACT GCCATGCTTG TGCCAAAGGA TCACACCACC ACCTTCGCTG 
TCAGATCCAC TTAAAGAGCT CTTTCGGCAA CAGGAAGTAG TAAGGATGAA ACTGCGTTTG 
CAACATAGTA TTGAAAGGGA AAAACTTATT GTATCCAATG AACAAGAAGT TCTGCGAGTT 
CATTACAGAG CTGCAAGAAC ACTGGCCAAT CAAACACTGC CATTTAGTGC TTGTACTGTC 
TTACTGGATG CAGAAGTGTA CAATGTGCCC TTGGACTCAC AGTCTGATGA CAGCAAAACT 
TCTGTGAGAG ATCGATTTAA TGCAAGACAG TTCATGTCCT GGTTGCAAGA TGTGGACGAT 
AAATTTGACA AGCTAAAGAC CTGTCTCTTA ATGAGGCAGC AACACGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGCTAGA ATGGCAGCTC AAACTCCAGG AGCTGGACCC TGCCACGTAT 
AAGTCTCTCA GTATTTATGA AATCCAGGAG TTTTATGTTC CCCTTGTTGA TGTTAATGAT 
GATTTTGAAC TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( XM_006523394.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523457.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X2, mRNA.

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CloneID	OMu52953
Clone ID Related Accession (Same CDS sequence)	XM_006523395.3 , XM_006523395.4 , XM_006523394.3 , XM_006523393.4 , XM_006523394.4 , XM_006523393.3
Accession Version	XM_006523394.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523394.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AAGAAGGGAG AGAATCTCGG TTGATTTTCT CAGCTCATCA 
ACCTTAAGGT CACCTTCACT GCCATGCTTG TGCCAAAGGA TCACACCACC ACCTTCGCTG 
TCAGATCCAC TTAAAGAGCT CTTTCGGCAA CAGGAAGTAG TAAGGATGAA ACTGCGTTTG 
CAACATAGTA TTGAAAGGGA AAAACTTATT GTATCCAATG AACAAGAAGT TCTGCGAGTT 
CATTACAGAG CTGCAAGAAC ACTGGCCAAT CAAACACTGC CATTTAGTGC TTGTACTGTC 
TTACTGGATG CAGAAGTGTA CAATGTGCCC TTGGACTCAC AGTCTGATGA CAGCAAAACT 
TCTGTGAGAG ATCGATTTAA TGCAAGACAG TTCATGTCCT GGTTGCAAGA TGTGGACGAT 
AAATTTGACA AGCTAAAGAC CTGTCTCTTA ATGAGGCAGC AACACGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGCTAGA ATGGCAGCTC AAACTCCAGG AGCTGGACCC TGCCACGTAT 
AAGTCTCTCA GTATTTATGA AATCCAGGAG TTTTATGTTC CCCTTGTTGA TGTTAATGAT 
GATTTTGAAC TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523457.1
CDS	539..6742
Translation	MPKSGFTKPVQSENSDSDSSMVERPYGRKSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSGDDESYTDSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTRRERISVDFLSSSTLRSPSLPCLCQRITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSLSIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_006523394.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523394.3

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AAGAAGGGAG AGAATCTCGG TTGATTTTCT CAGCTCATCA 
ACCTTAAGGT CACCTTCACT GCCATGCTTG TGCCAAAGGA TCACACCACC ACCTTCGCTG 
TCAGATCCAC TTAAAGAGCT CTTTCGGCAA CAGGAAGTAG TAAGGATGAA ACTGCGTTTG 
CAACATAGTA TTGAAAGGGA AAAACTTATT GTATCCAATG AACAAGAAGT TCTGCGAGTT 
CATTACAGAG CTGCAAGAAC ACTGGCCAAT CAAACACTGC CATTTAGTGC TTGTACTGTC 
TTACTGGATG CAGAAGTGTA CAATGTGCCC TTGGACTCAC AGTCTGATGA CAGCAAAACT 
TCTGTGAGAG ATCGATTTAA TGCAAGACAG TTCATGTCCT GGTTGCAAGA TGTGGACGAT 
AAATTTGACA AGCTAAAGAC CTGTCTCTTA ATGAGGCAGC AACACGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGCTAGA ATGGCAGCTC AAACTCCAGG AGCTGGACCC TGCCACGTAT 
AAGTCTCTCA GTATTTATGA AATCCAGGAG TTTTATGTTC CCCTTGTTGA TGTTAATGAT 
GATTTTGAAC TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( XM_006523393.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523456.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X1, mRNA.

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CloneID	OMu52953
Clone ID Related Accession (Same CDS sequence)	XM_006523395.3 , XM_006523395.4 , XM_006523394.3 , XM_006523393.4 , XM_006523394.4 , XM_006523393.3
Accession Version	XM_006523393.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523393.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AAGAAGGGAG AGAATCTCGG TTGATTTTCT CAGCTCATCA 
ACCTTAAGGT CACCTTCACT GCCATGCTTG TGCCAAAGGA TCACACCACC ACCTTCGCTG 
TCAGATCCAC TTAAAGAGCT CTTTCGGCAA CAGGAAGTAG TAAGGATGAA ACTGCGTTTG 
CAACATAGTA TTGAAAGGGA AAAACTTATT GTATCCAATG AACAAGAAGT TCTGCGAGTT 
CATTACAGAG CTGCAAGAAC ACTGGCCAAT CAAACACTGC CATTTAGTGC TTGTACTGTC 
TTACTGGATG CAGAAGTGTA CAATGTGCCC TTGGACTCAC AGTCTGATGA CAGCAAAACT 
TCTGTGAGAG ATCGATTTAA TGCAAGACAG TTCATGTCCT GGTTGCAAGA TGTGGACGAT 
AAATTTGACA AGCTAAAGAC CTGTCTCTTA ATGAGGCAGC AACACGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGCTAGA ATGGCAGCTC AAACTCCAGG AGCTGGACCC TGCCACGTAT 
AAGTCTCTCA GTATTTATGA AATCCAGGAG TTTTATGTTC CCCTTGTTGA TGTTAATGAT 
GATTTTGAAC TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523456.1
CDS	247..6450
Translation	MPKSGFTKPVQSENSDSDSSMVERPYGRKSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSGDDESYTDSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTRRERISVDFLSSSTLRSPSLPCLCQRITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSLSIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_006523393.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523393.3

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AAGAAGGGAG AGAATCTCGG TTGATTTTCT CAGCTCATCA 
ACCTTAAGGT CACCTTCACT GCCATGCTTG TGCCAAAGGA TCACACCACC ACCTTCGCTG 
TCAGATCCAC TTAAAGAGCT CTTTCGGCAA CAGGAAGTAG TAAGGATGAA ACTGCGTTTG 
CAACATAGTA TTGAAAGGGA AAAACTTATT GTATCCAATG AACAAGAAGT TCTGCGAGTT 
CATTACAGAG CTGCAAGAAC ACTGGCCAAT CAAACACTGC CATTTAGTGC TTGTACTGTC 
TTACTGGATG CAGAAGTGTA CAATGTGCCC TTGGACTCAC AGTCTGATGA CAGCAAAACT 
TCTGTGAGAG ATCGATTTAA TGCAAGACAG TTCATGTCCT GGTTGCAAGA TGTGGACGAT 
AAATTTGACA AGCTAAAGAC CTGTCTCTTA ATGAGGCAGC AACACGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGCTAGA ATGGCAGCTC AAACTCCAGG AGCTGGACCC TGCCACGTAT 
AAGTCTCTCA GTATTTATGA AATCCAGGAG TTTTATGTTC CCCTTGTTGA TGTTAATGAT 
GATTTTGAAC TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( XM_006523396.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523459.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X4, mRNA.

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CloneID	OMu52954
Clone ID Related Accession (Same CDS sequence)	XM_006523396.3 , XM_006523396.4
Accession Version	XM_006523396.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523396.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AAGAAGGGAG AGAATCTCGG TTGATTTTCT CAGCTCATCA 
ACCTTAAGGT CACCTTCACT GCCATGCTTG TGCCAAAGGA TCACACCACC ACCTTCGCTG 
TCAGATCCAC TTAAAGAGCT CTTTCGGCAA CAGGAAGTAG TAAGGATGAA ACTGCGTTTG 
CAACATAGTA TTGAAAGGGA AAAACTTATT GTATCCAATG AACAAGAAGT TCTGCGAGTT 
CATTACAGAG CTGCAAGAAC ACTGGCCAAT CAAACACTGC CATTTAGTGC TTGTACTGTC 
TTACTGGATG CAGAAGTGTA CAATGTGCCC TTGGACTCAC AGTCTGATGA CAGCAAAACT 
TCTGTGAGAG ATCGATTTAA TGCAAGACAG TTCATGTCCT GGTTGCAAGA TGTGGACGAT 
AAATTTGACA AGCTAAAGAA AGAAAATTTG ATGAGCTCGT GTGGCAGATT ACAAAAATTA 
AAAAAGATTA AATGTTCAAG CAAGCAAGAG AAAAGGAAAT CTGTAGAGCA TTTGGTTCTG 
ATGGTCCCGC CAGGGACCAG TCGAGGAAGT AGTACTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523459.1
CDS	247..6408
Translation	MPKSGFTKPVQSENSDSDSSMVERPYGRKSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSGDDESYTDSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTRRERISVDFLSSSTLRSPSLPCLCQRITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKKENLMSSCGRLQKLKKIKCSSKQEKRKSVEHLVLMVPPGTSRGSSTD

Target ORF information:

RefSeq Version	XM_006523396.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523396.3

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA AAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATAC 
TCAGAAAAAG AAGGTCCAGA GAAGAAGAAA ACAAAAAAAG AAGCTGGAAC TAAGAAGTCC 
ACCCCAGTTA GCATTCTTTT TGGTTATCCA CTTTCTGAAC GAAAGCAGAT GGCACTCCTA 
ATGCAGATGA CAGCAAGGGA CAACAGCCCA GATTCCACAC CAAGTCATCC GTCACAGGCA 
ACGCCAGCCC AGAAGAAAAC GCCCAGCTCT TCATCTCGAC AGAAAGATAA AATTAATAAA 
AGAAATGAGC GTGGCGAAAC TCCTTTACAC ATGGCCGCTA TTCGAGGAGA CGTGAAGCAA 
GTCAAAGAGT TAATTAGTTT AGGGGCAAAC GTCAATGTGA AGGACTTTGC AGGTTGGACC 
CCTTTGCATG AAGCTTGCAA TGTTGGCTAT TACGATGTTG CTAAGATACT GATAGCAGCT 
GGCGCAGACG TTAACACACA AGGACTGGAT GATGACACCC CGCTCCATGA CTCTGCCAGC 
AGTGGCCATA GAGACATAGT GAAGTTGTTG CTTCGTCATG GCGGGAATCC ATTCCAAGCT 
AATAAACATG GAGAACGGCC AGTGGATGTG GCAGAGACAG AGGAGTTGGA GTTGCTGCTA 
AAAAGAGAGG TGCCTTTGTC TGGCGATGAT GAAAGTTATA CAGATTCAGA AGAGGCTCAG 
TCTGTAAATC CTTCTAGTGT TGATGAAAAT ATTGACTCAG AGACAGAGAA AGACTCTTTC 
GTCTGTGGCA GTAAAATCCT CCCAAGCAAA GCCCCGCTGC CTTCTGCACT GGATGAGTAT 
GAGTTCAAAG ATGATGATGA AGAAGAGATA AGTAAGATGA TTGACGACAG GCACATTCTT 
AGGAAAGAAC AGCGGAGAGA GAATGAATCT GAAGCAGAAA GGAGTGGTCT GTTTGCAAAA 
CAGGAGAAAA CCTTCTATTC TAAGTCATTT AAAACAAAAA AACAGAAGCC GTCAAGGGTT 
CTGTGCTCCA GTACTGAAAG CTCTGATGAA GAGGGTCTTC AGAACAGAAA GATTTCTACT 
GCTTGTTCTG TTGCTGAATC ATCACATCCT GATATGCAAG CCAAAAAAGA ATATGAATAC 
AAACAGAAAG GCAAAGTTAA AAGAAAATTA AAAAATCAGA ATAAAAACAA AGAGAACCAA 
GAGCTGAAAC AAGAAAAAGA GGGGAAAGAA AATAGCAGGA TAGCAACCTT GACAGTTAAT 
ACTGCGCTGG ATTGTTCAGA AAAATACAGA GAGGAGGGGA ACTTCAGGAA ATCTTTTAGC 
CCAAAGGATG ATACTTCATT ACACTTATTT CATATTTCCA CTGGTAAATC TCCCAAACAT 
TCCTGTGGAT TAAGTGAAAA ACAGACACCA CTGAAGCAAG AACACACGAA AGCATGCATA 
TCCCCAGGAA GTTCTGAAAT GTCATTACAG CCTGATCTCA CTCGGTATGA TAATGCAGAA 
CCTGAAAGTT CAAATGTCAA ATCTTATAAG CACAAGGAAA AAAACAAACA GCAGAAAGAT 
TTTCACATAG AGTTTGGTGA AAAATCAAAT GCCAAAATTA AAGATGAAGA TCATAATCCA 
GCATTTGAAA ACTCAGACTG CACACTGAAG AAGATGGATA AAGAGGGTAA AACATTAAAA 
AAACATAAAT TAAAACATAA AGAGAAGGAT AAAGAAAAGC ACAAAAAGGA AGTTGAAGGT 
GAAAAGGAAA AATATAAAAA TAAAGACAAT GCTAAAGAGC TACAGAGGAG CATCGAATTT 
GACAGAGAGT TTTGGAAAGA AAATTTTTTT AAAAGTGATG AAACTGAGGA TCTCTTTTTA 
ACTATGGAAC ATGAGTCCAT AATAGAAAAA AAGACAAAAA TGGAAAAAAA CATGAAAGAT 
GATAGGTCAG CTAAAGAAAA GCATGTCTCA AAAGAAAGGA ACTTTAAAGA AGAACGGGAA 
AAGACTAAAA AGGAAAATGA AAAATCTCTT AGGGAAGAAA AGGTAAAAGA TCCGAAAGAA 
GAAAAAGACA GTGTATCCAC AGAGAAGGAG GCCGAGTGCT GTTCGGTGGG TGTCAGTGTG 
AGCCAGGAAG CTGCAGGGCT GCATTCATCA GAAAAGGAAA TGGATGCTGA GAAGCAAGAA 
AGACACACAA AAGAGAGGGA AAAGTCTGAT CGGCGCTTTC AGACTAAGGA GAAAGAGCTT 
GACAGGAACG AGAGGAAATT TTCTGAAAAA GAAAAGAAGA TAAAACATGA ACATAAGTCA 
GAAAGAGAGA AGCTGGATTT CAGTGACTGC ACTGACAGAG TGAGAGAGAA GGACAAGCTG 
TACTCACACC AGACAGAAAG GTGCCACAAG GACAGCGAGA AGCTGAAAAA CACTGTCAGA 
AAGACTGATG ACAGAGAGAG GAACAGAGAA AAGGCGGATA GAAAGCACGA CAGAGAGAAA 
CCTGAACGAG AGAGGTACTT AGCAGAAAAC AAAGAAAAGC ACTTAGAGAA AAGAAAGCAA 
TCTGACAGCA GTGAGTATTC CAAATCAGAG AAGGTCAGAA CTAAAGACAG AGACAGGGAA 
GGAGAGAAGA AGGAGAAACT TAGAGATAAA GAAAGTGTAA ATGTAACTAA CTTGAAACAT 
TTGCAGGAAG AGAGAAAGTC CAGTACAGGA GACAGCAGTG GTAAAACCCA GCATGAGAAA 
GCTCTGTCCC TTAAAGAAAG GGCAAGAGAT GAGCCTTTGA AAACTCCAGA TGGAAAAGAG 
AAAGATAAAA AGGATATAGA CAGATACAAA GAGCGAGACA AGCGTAAAGA TAAAGCCCAG 
CTAAATACTT TAATCAGACT AAAATCTGAA ACAGAGAAGC TTAAACCTAA ATTATCACCA 
GCATCAAAAG ATACTCGACC TAAAGAAAAG AGGTTAGTAA ATGATGATTT AATGCAGACA 
AGTTTTGAAC GAATGCTTAG CCTTAAAGAC CTAGAAATAG AACAGTGGCA CAGAAAACAT 
AAGGAAAAAA TTAAGCAAAA GGAAAAAGAA CGATTACGCA ATCGTACTTG TTTAGAACTT 
AAAGTAAAAG ATAAAGAAAA AACAAAACAT GCACCCACAG AATCCAAAAG TAAAGAACTT 
ACCAGGTCAA GAAGTTCAGA GTTGACTGAT GTTTATAGCA AGGAGAAACA ATCTAAAGAT 
GTTGGCAGTA ATCGCTCACA ATCTGTTGAC AACAGAAATG TATTGAATTT AGGAAAGCCA 
TCCTACATTT CAGAGAGTGG CTCCAACCGG TCTCCTAGGT CGGAAGGTGA GAGGCTGGGT 
CTTAGCTCTA GGTCTGTGTC CATGCTTTCT GTTGCTAGTT CAGAAGACTC TTGCCATACA 
GTGACAACCC CAAGGCCTCT CGTTGAGTAT GACTCTGACT TTATGTTAGA GGGCTCGGAA 
TCTCAGTTGT CATTTTCTCA GTCACCTTTT TTGCCAAATG CCAAATCTCC AGCTCTTTAT 
GAAAGAGAGC TGGAGGGTCT CACTGAACTA CCAGACCGAC TTAAACCACC ATATACAAAC 
AGACTTCCAG TGTGCCATCT CAGGTCATCT TCTGTGGAAG ATGTTAAACT AATTACTAAT 
GAGGTAAGAC CTGCTGTAGA AATTAGAAGA TGCAGTATGC CCTCAGTTAT CTGTGAGCCC 
ATAAAACATT TCCAAGTATC AGAAGAAAGC AGTCAAGGCA ACTTAGCTGT TCCCAGAGAG 
ACCTGTCTGT CTCCCAAGCC TGAAGTGTCT TCAGCAGTAC CTGAGAGGGG CCTTTCGCAT 
GTGTCTGACT TACATCCTAA CTGTACAGGC TCTCCAACGA GGTCTGCAAA CAGCAGCTAT 
ATTTCATCTG ATGTAAATGT AATCAAGAGT GCAACTCTTG TGGGTACACT GATTGACAGT 
TCGATGCATT TGGAACCGTC TAATCAGGTT GGAGTGATTC CAAGTAAATC ATGGGAGACG 
CCTGTTGATA AACTGGAGTC TTTAAGCACC AATGACTTCA TCTGTCCAGA TTCTAATATA 
TCTGATCAAG ATTCCTCTGC TCAGAGTTTT TATAATTCAG AAAACAAAAT GTTAAGAGAA 
CAAAATACTG ACTGCTTACC TCCGCACCAG CCTGAACTGC CAGGAAACTC ATGTGCTCAG 
GACCCAGCAT CCTTTCTGTC TTCACCTCAG CCCTGCTCTT TTTCCAACCA ACCACTGGCA 
GGTGCTGACT CTGTTCCGAA ACCCGTGTCT TTGTCATATG TTGTCAGTCA AGAGCCAGGT 
GTTTTACAAC AGAAAAGTGC AGCTCAGATG GCCAGTTCTG TTTTAGATAG TGATAATAAC 
TTTCCAGAGG ATGTGGAAAA TACATTTGTC TTAGCAGACA TTCAGAAGAC AAATTCTTTT 
TTACCAGCTT ACTCTGAAAG CACTGTGCAA GAAGCACTCC CAAGCTTTGA GAAGGCCAAT 
ACTTTGCTTG TGTTGCCATC AGAAAAGGAC TTCAATGGGA GTGATACTGC TCAGCCAAAC 
ACACATTATG CATTTAGCAA ACTCACATAC AAGTCTTCCA ATAGCCAGGA AATTGAGAAG 
GGCCCAGATG ATACTCAAAT CATTTCACAT GAAAAGGAAA ACAAATTACA AAGTTTGGTC 
TTAACTCAGT TGAGTAAATG TGATTCTGAT TTGTATGAGA TGAATGCAGG GCTGCCAAAA 
GGAAACCTGA ATGAACAGGA TAACCCAAAG CATGGTCTTG ACAGCGAAAA GTACTCGCTT 
TCCACTGAAG ATGAAGAATC TCAGCAGAGC ACCTTATCAA GTGTGGAAAA CCATTCACAG 
CAGTCGGCCC AACCAGAGAT GCATAAATAT GACCAGCTTA GTAAAGTAGA ACTAGAAGAA 
AATGCCGAAG ACGATAAAAC TGAAAACCAG ACCCCTCAAA GAGTGACTAG AAACAAAGCA 
AGCACAGTGG TCAATCCAAG CAAGCCCATG CTTGCTGGCT GTGCCCTTGT AGCAGAGAAA 
GATAACGAGT CCTCCCCTAG GGGACGAATC AGACTCACTG AAGAGGATGA TCCTCAGATC 
CACCATCCTC GGAAGAGGAA AGTGTCACGG GTTCCTCAGC CTGTGCAAGT GAGCCCCTCT 
TTACTGCAAG CAAAGGAGAA AACTCAGCAG TCTCTAGCAG CCATTGTAGA TTCTCTGAAG 
TTAGATGAGA TTGAGCCATA CAGTTCTGAG AGAGCAAATC CATATTTTGA ATATTTGCAC 
ATAAGAAAAA AAATTGAAGA AAAACGCAAG TTGCTATGTA GCGTTATCCC TCAAGCTCCT 
CAGTATTATG ATGAATACGT AACGTTTAAT GGATCCTATC TCCTGGATGG AAACCCCTTA 
AGCAAGATTT GTATTCCTAC AAGAAGGGAG AGAATCTCGG TTGATTTTCT CAGCTCATCA 
ACCTTAAGGT CACCTTCACT GCCATGCTTG TGCCAAAGGA TCACACCACC ACCTTCGCTG 
TCAGATCCAC TTAAAGAGCT CTTTCGGCAA CAGGAAGTAG TAAGGATGAA ACTGCGTTTG 
CAACATAGTA TTGAAAGGGA AAAACTTATT GTATCCAATG AACAAGAAGT TCTGCGAGTT 
CATTACAGAG CTGCAAGAAC ACTGGCCAAT CAAACACTGC CATTTAGTGC TTGTACTGTC 
TTACTGGATG CAGAAGTGTA CAATGTGCCC TTGGACTCAC AGTCTGATGA CAGCAAAACT 
TCTGTGAGAG ATCGATTTAA TGCAAGACAG TTCATGTCCT GGTTGCAAGA TGTGGACGAT 
AAATTTGACA AGCTAAAGAA AGAAAATTTG ATGAGCTCGT GTGGCAGATT ACAAAAATTA 
AAAAAGATTA AATGTTCAAG CAAGCAAGAG AAAAGGAAAT CTGTAGAGCA TTTGGTTCTG 
ATGGTCCCGC CAGGGACCAG TCGAGGAAGT AGTACTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( XM_006523397.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523460.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X5, mRNA.

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CloneID	OMu52955
Clone ID Related Accession (Same CDS sequence)	XM_030249418.1 , XM_006523397.4 , XM_030249417.1 , XM_006523397.3
Accession Version	XM_006523397.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523397.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGAAAAA 
GAAGGTCCAG AGAAGAAGAA AACAAAAAAA GAAGCTGGAA CTAAGAAGTC CACCCCAGTT 
AGCATTCTTT TTGGTTATCC ACTTTCTGAA CGAAAGCAGA TGGCACTCCT AATGCAGATG 
ACAGCAAGGG ACAACAGCCC AGATTCCACA CCAAGTCATC CGTCACAGGC AACGCCAGCC 
CAGAAGAAAA CGCCCAGCTC TTCATCTCGA CAGAAAGATA AAATTAATAA AAGAAATGAG 
CGTGGCGAAA CTCCTTTACA CATGGCCGCT ATTCGAGGAG ACGTGAAGCA AGTCAAAGAG 
TTAATTAGTT TAGGGGCAAA CGTCAATGTG AAGGACTTTG CAGGTTGGAC CCCTTTGCAT 
GAAGCTTGCA ATGTTGGCTA TTACGATGTT GCTAAGATAC TGATAGCAGC TGGCGCAGAC 
GTTAACACAC AAGGACTGGA TGATGACACC CCGCTCCATG ACTCTGCCAG CAGTGGCCAT 
AGAGACATAG TGAAGTTGTT GCTTCGTCAT GGCGGGAATC CATTCCAAGC TAATAAACAT 
GGAGAACGGC CAGTGGATGT GGCAGAGACA GAGGAGTTGG AGTTGCTGCT AAAAAGAGAG 
GTGCCTTTGT CTGGCGATGA TGAAAGTTAT ACAGATTCAG AAGAGGCTCA GTCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCA GAGACAGAGA AAGACTCTTT CGTCTGTGGC 
AGTAAAATCC TCCCAAGCAA AGCCCCGCTG CCTTCTGCAC TGGATGAGTA TGAGTTCAAA 
GATGATGATG AAGAAGAGAT AAGTAAGATG ATTGACGACA GGCACATTCT TAGGAAAGAA 
CAGCGGAGAG AGAATGAATC TGAAGCAGAA AGGAGTGGTC TGTTTGCAAA ACAGGAGAAA 
ACCTTCTATT CTAAGTCATT TAAAACAAAA AAACAGAAGC CGTCAAGGGT TCTGTGCTCC 
AGTACTGAAA GCTCTGATGA AGAGGGTCTT CAGAACAGAA AGATTTCTAC TGCTTGTTCT 
GTTGCTGAAT CATCACATCC TGATATGCAA GCCAAAAAAG AATATGAATA CAAACAGAAA 
GGCAAAGTTA AAAGAAAATT AAAAAATCAG AATAAAAACA AAGAGAACCA AGAGCTGAAA 
CAAGAAAAAG AGGGGAAAGA AAATAGCAGG ATAGCAACCT TGACAGTTAA TACTGCGCTG 
GATTGTTCAG AAAAATACAG AGAGGAGGGG AACTTCAGGA AATCTTTTAG CCCAAAGGAT 
GATACTTCAT TACACTTATT TCATATTTCC ACTGGTAAAT CTCCCAAACA TTCCTGTGGA 
TTAAGTGAAA AACAGACACC ACTGAAGCAA GAACACACGA AAGCATGCAT ATCCCCAGGA 
AGTTCTGAAA TGTCATTACA GCCTGATCTC ACTCGGTATG ATAATGCAGA ACCTGAAAGT 
TCAAATGTCA AATCTTATAA GCACAAGGAA AAAAACAAAC AGCAGAAAGA TTTTCACATA 
GAGTTTGGTG AAAAATCAAA TGCCAAAATT AAAGATGAAG ATCATAATCC AGCATTTGAA 
AACTCAGACT GCACACTGAA GAAGATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAA 
TTAAAACATA AAGAGAAGGA TAAAGAAAAG CACAAAAAGG AAGTTGAAGG TGAAAAGGAA 
AAATATAAAA ATAAAGACAA TGCTAAAGAG CTACAGAGGA GCATCGAATT TGACAGAGAG 
TTTTGGAAAG AAAATTTTTT TAAAAGTGAT GAAACTGAGG ATCTCTTTTT AACTATGGAA 
CATGAGTCCA TAATAGAAAA AAAGACAAAA ATGGAAAAAA ACATGAAAGA TGATAGGTCA 
GCTAAAGAAA AGCATGTCTC AAAAGAAAGG AACTTTAAAG AAGAACGGGA AAAGACTAAA 
AAGGAAAATG AAAAATCTCT TAGGGAAGAA AAGGTAAAAG ATCCGAAAGA AGAAAAAGAC 
AGTGTATCCA CAGAGAAGGA GGCCGAGTGC TGTTCGGTGG GTGTCAGTGT GAGCCAGGAA 
GCTGCAGGGC TGCATTCATC AGAAAAGGAA ATGGATGCTG AGAAGCAAGA AAGACACACA 
AAAGAGAGGG AAAAGTCTGA TCGGCGCTTT CAGACTAAGG AGAAAGAGCT TGACAGGAAC 
GAGAGGAAAT TTTCTGAAAA AGAAAAGAAG ATAAAACATG AACATAAGTC AGAAAGAGAG 
AAGCTGGATT TCAGTGACTG CACTGACAGA GTGAGAGAGA AGGACAAGCT GTACTCACAC 
CAGACAGAAA GGTGCCACAA GGACAGCGAG AAGCTGAAAA ACACTGTCAG AAAGACTGAT 
GACAGAGAGA GGAACAGAGA AAAGGCGGAT AGAAAGCACG ACAGAGAGAA ACCTGAACGA 
GAGAGGTACT TAGCAGAAAA CAAAGAAAAG CACTTAGAGA AAAGAAAGCA ATCTGACAGC 
AGTGAGTATT CCAAATCAGA GAAGGTCAGA ACTAAAGACA GAGACAGGGA AGGAGAGAAG 
AAGGAGAAAC TTAGAGATAA AGAAAGTGTA AATGTAACTA ACTTGAAACA TTTGCAGGAA 
GAGAGAAAGT CCAGTACAGG AGACAGCAGT GGTAAAACCC AGCATGAGAA AGCTCTGTCC 
CTTAAAGAAA GGGCAAGAGA TGAGCCTTTG AAAACTCCAG ATGGAAAAGA GAAAGATAAA 
AAGGATATAG ACAGATACAA AGAGCGAGAC AAGCGTAAAG ATAAAGCCCA GCTAAATACT 
TTAATCAGAC TAAAATCTGA AACAGAGAAG CTTAAACCTA AATTATCACC AGCATCAAAA 
GATACTCGAC CTAAAGAAAA GAGGTTAGTA AATGATGATT TAATGCAGAC AAGTTTTGAA 
CGAATGCTTA GCCTTAAAGA CCTAGAAATA GAACAGTGGC ACAGAAAACA TAAGGAAAAA 
ATTAAGCAAA AGGAAAAAGA ACGATTACGC AATCGTACTT GTTTAGAACT TAAAGTAAAA 
GATAAAGAAA AAACAAAACA TGCACCCACA GAATCCAAAA GTAAAGAACT TACCAGGTCA 
AGAAGTTCAG AGTTGACTGA TGTTTATAGC AAGGAGAAAC AATCTAAAGA TGTTGGCAGT 
AATCGCTCAC AATCTGTTGA CAACAGAAAT GTATTGAATT TAGGAAAGCC ATCCTACATT 
TCAGAGAGTG GCTCCAACCG GTCTCCTAGG TCGGAAGGTG AGAGGCTGGG TCTTAGCTCT 
AGGTCTGTGT CCATGCTTTC TGTTGCTAGT TCAGAAGACT CTTGCCATAC AGTGACAACC 
CCAAGGCCTC TCGTTGAGTA TGACTCTGAC TTTATGTTAG AGGGCTCGGA ATCTCAGTTG 
TCATTTTCTC AGTCACCTTT TTTGCCAAAT GCCAAATCTC CAGCTCTTTA TGAAAGAGAG 
CTGGAGGGTC TCACTGAACT ACCAGACCGA CTTAAACCAC CATATACAAA CAGACTTCCA 
GTGTGCCATC TCAGGTCATC TTCTGTGGAA GATGTTAAAC TAATTACTAA TGAGGTAAGA 
CCTGCTGTAG AAATTAGAAG ATGCAGTATG CCCTCAGTTA TCTGTGAGCC CATAAAACAT 
TTCCAAGTAT CAGAAGAAAG CAGTCAAGGC AACTTAGCTG TTCCCAGAGA GACCTGTCTG 
TCTCCCAAGC CTGAAGTGTC TTCAGCAGTA CCTGAGAGGG GCCTTTCGCA TGTGTCTGAC 
TTACATCCTA ACTGTACAGG CTCTCCAACG AGGTCTGCAA ACAGCAGCTA TATTTCATCT 
GATGTAAATG TAATCAAGAG TGCAACTCTT GTGGGTACAC TGATTGACAG TTCGATGCAT 
TTGGAACCGT CTAATCAGGT TGGAGTGATT CCAAGTAAAT CATGGGAGAC GCCTGTTGAT 
AAACTGGAGT CTTTAAGCAC CAATGACTTC ATCTGTCCAG ATTCTAATAT ATCTGATCAA 
GATTCCTCTG CTCAGAGTTT TTATAATTCA GAAAACAAAA TGTTAAGAGA ACAAAATACT 
GACTGCTTAC CTCCGCACCA GCCTGAACTG CCAGGAAACT CATGTGCTCA GGACCCAGCA 
TCCTTTCTGT CTTCACCTCA GCCCTGCTCT TTTTCCAACC AACCACTGGC AGGTGCTGAC 
TCTGTTCCGA AACCCGTGTC TTTGTCATAT GTTGTCAGTC AAGAGCCAGG TGTTTTACAA 
CAGAAAAGTG CAGCTCAGAT GGCCAGTTCT GTTTTAGATA GTGATAATAA CTTTCCAGAG 
GATGTGGAAA ATACATTTGT CTTAGCAGAC ATTCAGAAGA CAAATTCTTT TTTACCAGCT 
TACTCTGAAA GCACTGTGCA AGAAGCACTC CCAAGCTTTG AGAAGGCCAA TACTTTGCTT 
GTGTTGCCAT CAGAAAAGGA CTTCAATGGG AGTGATACTG CTCAGCCAAA CACACATTAT 
GCATTTAGCA AACTCACATA CAAGTCTTCC AATAGCCAGG AAATTGAGAA GGGCCCAGAT 
GATACTCAAA TCATTTCACA TGAAAAGGAA AACAAATTAC AAAGTTTGGT CTTAACTCAG 
TTGAGTAAAT GTGATTCTGA TTTGTATGAG ATGAATGCAG GGCTGCCAAA AGGAAACCTG 
AATGAACAGG ATAACCCAAA GCATGGTCTT GACAGCGAAA AGTACTCGCT TTCCACTGAA 
GATGAAGAAT CTCAGCAGAG CACCTTATCA AGTGTGGAAA ACCATTCACA GCAGTCGGCC 
CAACCAGAGA TGCATAAATA TGACCAGCTT AGTAAAGTAG AACTAGAAGA AAATGCCGAA 
GACGATAAAA CTGAAAACCA GACCCCTCAA AGAGTGACTA GAAACAAAGC AAGCACAGTG 
GTCAATCCAA GCAAGCCCAT GCTTGCTGGC TGTGCCCTTG TAGCAGAGAA AGATAACGAG 
TCCTCCCCTA GGGGACGAAT CAGACTCACT GAAGAGGATG ATCCTCAGAT CCACCATCCT 
CGGAAGAGGA AAGTGTCACG GGTTCCTCAG CCTGTGCAAG TGAGCCCCTC TTTACTGCAA 
GCAAAGGAGA AAACTCAGCA GTCTCTAGCA GCCATTGTAG ATTCTCTGAA GTTAGATGAG 
ATTGAGCCAT ACAGTTCTGA GAGAGCAAAT CCATATTTTG AATATTTGCA CATAAGAAAA 
AAAATTGAAG AAAAACGCAA GTTGCTATGT AGCGTTATCC CTCAAGCTCC TCAGTATTAT 
GATGAATACG TAACGTTTAA TGGATCCTAT CTCCTGGATG GAAACCCCTT AAGCAAGATT 
TGTATTCCTA CAAGAAGGGA GAGAATCTCG GTTGATTTTC TCAGCTCATC AACCTTAAGG 
TCACCTTCAC TGCCATGCTT GTGCCAAAGG ATCACACCAC CACCTTCGCT GTCAGATCCA 
CTTAAAGAGC TCTTTCGGCA ACAGGAAGTA GTAAGGATGA AACTGCGTTT GCAACATAGT 
ATTGAAAGGG AAAAACTTAT TGTATCCAAT GAACAAGAAG TTCTGCGAGT TCATTACAGA 
GCTGCAAGAA CACTGGCCAA TCAAACACTG CCATTTAGTG CTTGTACTGT CTTACTGGAT 
GCAGAAGTGT ACAATGTGCC CTTGGACTCA CAGTCTGATG ACAGCAAAAC TTCTGTGAGA 
GATCGATTTA ATGCAAGACA GTTCATGTCC TGGTTGCAAG ATGTGGACGA TAAATTTGAC 
AAGCTAAAGA CCTGTCTCTT AATGAGGCAG CAACACGAAG CTGCAGCTTT AAATGCCGTC 
CAGAGGCTAG AATGGCAGCT CAAACTCCAG GAGCTGGACC CTGCCACGTA TAAGTCTCTC 
AGTATTTATG AAATCCAGGA GTTTTATGTT CCCCTTGTTG ATGTTAATGA TGATTTTGAA 
CTGACTCCTA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523460.1
CDS	258..6392
Translation	MPKSGFTKPVQSENSDSDSSMVERPYGRKSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSGDDESYTDSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTRRERISVDFLSSSTLRSPSLPCLCQRITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSLSIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_006523397.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523397.3

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGAAAAA 
GAAGGTCCAG AGAAGAAGAA AACAAAAAAA GAAGCTGGAA CTAAGAAGTC CACCCCAGTT 
AGCATTCTTT TTGGTTATCC ACTTTCTGAA CGAAAGCAGA TGGCACTCCT AATGCAGATG 
ACAGCAAGGG ACAACAGCCC AGATTCCACA CCAAGTCATC CGTCACAGGC AACGCCAGCC 
CAGAAGAAAA CGCCCAGCTC TTCATCTCGA CAGAAAGATA AAATTAATAA AAGAAATGAG 
CGTGGCGAAA CTCCTTTACA CATGGCCGCT ATTCGAGGAG ACGTGAAGCA AGTCAAAGAG 
TTAATTAGTT TAGGGGCAAA CGTCAATGTG AAGGACTTTG CAGGTTGGAC CCCTTTGCAT 
GAAGCTTGCA ATGTTGGCTA TTACGATGTT GCTAAGATAC TGATAGCAGC TGGCGCAGAC 
GTTAACACAC AAGGACTGGA TGATGACACC CCGCTCCATG ACTCTGCCAG CAGTGGCCAT 
AGAGACATAG TGAAGTTGTT GCTTCGTCAT GGCGGGAATC CATTCCAAGC TAATAAACAT 
GGAGAACGGC CAGTGGATGT GGCAGAGACA GAGGAGTTGG AGTTGCTGCT AAAAAGAGAG 
GTGCCTTTGT CTGGCGATGA TGAAAGTTAT ACAGATTCAG AAGAGGCTCA GTCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCA GAGACAGAGA AAGACTCTTT CGTCTGTGGC 
AGTAAAATCC TCCCAAGCAA AGCCCCGCTG CCTTCTGCAC TGGATGAGTA TGAGTTCAAA 
GATGATGATG AAGAAGAGAT AAGTAAGATG ATTGACGACA GGCACATTCT TAGGAAAGAA 
CAGCGGAGAG AGAATGAATC TGAAGCAGAA AGGAGTGGTC TGTTTGCAAA ACAGGAGAAA 
ACCTTCTATT CTAAGTCATT TAAAACAAAA AAACAGAAGC CGTCAAGGGT TCTGTGCTCC 
AGTACTGAAA GCTCTGATGA AGAGGGTCTT CAGAACAGAA AGATTTCTAC TGCTTGTTCT 
GTTGCTGAAT CATCACATCC TGATATGCAA GCCAAAAAAG AATATGAATA CAAACAGAAA 
GGCAAAGTTA AAAGAAAATT AAAAAATCAG AATAAAAACA AAGAGAACCA AGAGCTGAAA 
CAAGAAAAAG AGGGGAAAGA AAATAGCAGG ATAGCAACCT TGACAGTTAA TACTGCGCTG 
GATTGTTCAG AAAAATACAG AGAGGAGGGG AACTTCAGGA AATCTTTTAG CCCAAAGGAT 
GATACTTCAT TACACTTATT TCATATTTCC ACTGGTAAAT CTCCCAAACA TTCCTGTGGA 
TTAAGTGAAA AACAGACACC ACTGAAGCAA GAACACACGA AAGCATGCAT ATCCCCAGGA 
AGTTCTGAAA TGTCATTACA GCCTGATCTC ACTCGGTATG ATAATGCAGA ACCTGAAAGT 
TCAAATGTCA AATCTTATAA GCACAAGGAA AAAAACAAAC AGCAGAAAGA TTTTCACATA 
GAGTTTGGTG AAAAATCAAA TGCCAAAATT AAAGATGAAG ATCATAATCC AGCATTTGAA 
AACTCAGACT GCACACTGAA GAAGATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAA 
TTAAAACATA AAGAGAAGGA TAAAGAAAAG CACAAAAAGG AAGTTGAAGG TGAAAAGGAA 
AAATATAAAA ATAAAGACAA TGCTAAAGAG CTACAGAGGA GCATCGAATT TGACAGAGAG 
TTTTGGAAAG AAAATTTTTT TAAAAGTGAT GAAACTGAGG ATCTCTTTTT AACTATGGAA 
CATGAGTCCA TAATAGAAAA AAAGACAAAA ATGGAAAAAA ACATGAAAGA TGATAGGTCA 
GCTAAAGAAA AGCATGTCTC AAAAGAAAGG AACTTTAAAG AAGAACGGGA AAAGACTAAA 
AAGGAAAATG AAAAATCTCT TAGGGAAGAA AAGGTAAAAG ATCCGAAAGA AGAAAAAGAC 
AGTGTATCCA CAGAGAAGGA GGCCGAGTGC TGTTCGGTGG GTGTCAGTGT GAGCCAGGAA 
GCTGCAGGGC TGCATTCATC AGAAAAGGAA ATGGATGCTG AGAAGCAAGA AAGACACACA 
AAAGAGAGGG AAAAGTCTGA TCGGCGCTTT CAGACTAAGG AGAAAGAGCT TGACAGGAAC 
GAGAGGAAAT TTTCTGAAAA AGAAAAGAAG ATAAAACATG AACATAAGTC AGAAAGAGAG 
AAGCTGGATT TCAGTGACTG CACTGACAGA GTGAGAGAGA AGGACAAGCT GTACTCACAC 
CAGACAGAAA GGTGCCACAA GGACAGCGAG AAGCTGAAAA ACACTGTCAG AAAGACTGAT 
GACAGAGAGA GGAACAGAGA AAAGGCGGAT AGAAAGCACG ACAGAGAGAA ACCTGAACGA 
GAGAGGTACT TAGCAGAAAA CAAAGAAAAG CACTTAGAGA AAAGAAAGCA ATCTGACAGC 
AGTGAGTATT CCAAATCAGA GAAGGTCAGA ACTAAAGACA GAGACAGGGA AGGAGAGAAG 
AAGGAGAAAC TTAGAGATAA AGAAAGTGTA AATGTAACTA ACTTGAAACA TTTGCAGGAA 
GAGAGAAAGT CCAGTACAGG AGACAGCAGT GGTAAAACCC AGCATGAGAA AGCTCTGTCC 
CTTAAAGAAA GGGCAAGAGA TGAGCCTTTG AAAACTCCAG ATGGAAAAGA GAAAGATAAA 
AAGGATATAG ACAGATACAA AGAGCGAGAC AAGCGTAAAG ATAAAGCCCA GCTAAATACT 
TTAATCAGAC TAAAATCTGA AACAGAGAAG CTTAAACCTA AATTATCACC AGCATCAAAA 
GATACTCGAC CTAAAGAAAA GAGGTTAGTA AATGATGATT TAATGCAGAC AAGTTTTGAA 
CGAATGCTTA GCCTTAAAGA CCTAGAAATA GAACAGTGGC ACAGAAAACA TAAGGAAAAA 
ATTAAGCAAA AGGAAAAAGA ACGATTACGC AATCGTACTT GTTTAGAACT TAAAGTAAAA 
GATAAAGAAA AAACAAAACA TGCACCCACA GAATCCAAAA GTAAAGAACT TACCAGGTCA 
AGAAGTTCAG AGTTGACTGA TGTTTATAGC AAGGAGAAAC AATCTAAAGA TGTTGGCAGT 
AATCGCTCAC AATCTGTTGA CAACAGAAAT GTATTGAATT TAGGAAAGCC ATCCTACATT 
TCAGAGAGTG GCTCCAACCG GTCTCCTAGG TCGGAAGGTG AGAGGCTGGG TCTTAGCTCT 
AGGTCTGTGT CCATGCTTTC TGTTGCTAGT TCAGAAGACT CTTGCCATAC AGTGACAACC 
CCAAGGCCTC TCGTTGAGTA TGACTCTGAC TTTATGTTAG AGGGCTCGGA ATCTCAGTTG 
TCATTTTCTC AGTCACCTTT TTTGCCAAAT GCCAAATCTC CAGCTCTTTA TGAAAGAGAG 
CTGGAGGGTC TCACTGAACT ACCAGACCGA CTTAAACCAC CATATACAAA CAGACTTCCA 
GTGTGCCATC TCAGGTCATC TTCTGTGGAA GATGTTAAAC TAATTACTAA TGAGGTAAGA 
CCTGCTGTAG AAATTAGAAG ATGCAGTATG CCCTCAGTTA TCTGTGAGCC CATAAAACAT 
TTCCAAGTAT CAGAAGAAAG CAGTCAAGGC AACTTAGCTG TTCCCAGAGA GACCTGTCTG 
TCTCCCAAGC CTGAAGTGTC TTCAGCAGTA CCTGAGAGGG GCCTTTCGCA TGTGTCTGAC 
TTACATCCTA ACTGTACAGG CTCTCCAACG AGGTCTGCAA ACAGCAGCTA TATTTCATCT 
GATGTAAATG TAATCAAGAG TGCAACTCTT GTGGGTACAC TGATTGACAG TTCGATGCAT 
TTGGAACCGT CTAATCAGGT TGGAGTGATT CCAAGTAAAT CATGGGAGAC GCCTGTTGAT 
AAACTGGAGT CTTTAAGCAC CAATGACTTC ATCTGTCCAG ATTCTAATAT ATCTGATCAA 
GATTCCTCTG CTCAGAGTTT TTATAATTCA GAAAACAAAA TGTTAAGAGA ACAAAATACT 
GACTGCTTAC CTCCGCACCA GCCTGAACTG CCAGGAAACT CATGTGCTCA GGACCCAGCA 
TCCTTTCTGT CTTCACCTCA GCCCTGCTCT TTTTCCAACC AACCACTGGC AGGTGCTGAC 
TCTGTTCCGA AACCCGTGTC TTTGTCATAT GTTGTCAGTC AAGAGCCAGG TGTTTTACAA 
CAGAAAAGTG CAGCTCAGAT GGCCAGTTCT GTTTTAGATA GTGATAATAA CTTTCCAGAG 
GATGTGGAAA ATACATTTGT CTTAGCAGAC ATTCAGAAGA CAAATTCTTT TTTACCAGCT 
TACTCTGAAA GCACTGTGCA AGAAGCACTC CCAAGCTTTG AGAAGGCCAA TACTTTGCTT 
GTGTTGCCAT CAGAAAAGGA CTTCAATGGG AGTGATACTG CTCAGCCAAA CACACATTAT 
GCATTTAGCA AACTCACATA CAAGTCTTCC AATAGCCAGG AAATTGAGAA GGGCCCAGAT 
GATACTCAAA TCATTTCACA TGAAAAGGAA AACAAATTAC AAAGTTTGGT CTTAACTCAG 
TTGAGTAAAT GTGATTCTGA TTTGTATGAG ATGAATGCAG GGCTGCCAAA AGGAAACCTG 
AATGAACAGG ATAACCCAAA GCATGGTCTT GACAGCGAAA AGTACTCGCT TTCCACTGAA 
GATGAAGAAT CTCAGCAGAG CACCTTATCA AGTGTGGAAA ACCATTCACA GCAGTCGGCC 
CAACCAGAGA TGCATAAATA TGACCAGCTT AGTAAAGTAG AACTAGAAGA AAATGCCGAA 
GACGATAAAA CTGAAAACCA GACCCCTCAA AGAGTGACTA GAAACAAAGC AAGCACAGTG 
GTCAATCCAA GCAAGCCCAT GCTTGCTGGC TGTGCCCTTG TAGCAGAGAA AGATAACGAG 
TCCTCCCCTA GGGGACGAAT CAGACTCACT GAAGAGGATG ATCCTCAGAT CCACCATCCT 
CGGAAGAGGA AAGTGTCACG GGTTCCTCAG CCTGTGCAAG TGAGCCCCTC TTTACTGCAA 
GCAAAGGAGA AAACTCAGCA GTCTCTAGCA GCCATTGTAG ATTCTCTGAA GTTAGATGAG 
ATTGAGCCAT ACAGTTCTGA GAGAGCAAAT CCATATTTTG AATATTTGCA CATAAGAAAA 
AAAATTGAAG AAAAACGCAA GTTGCTATGT AGCGTTATCC CTCAAGCTCC TCAGTATTAT 
GATGAATACG TAACGTTTAA TGGATCCTAT CTCCTGGATG GAAACCCCTT AAGCAAGATT 
TGTATTCCTA CAAGAAGGGA GAGAATCTCG GTTGATTTTC TCAGCTCATC AACCTTAAGG 
TCACCTTCAC TGCCATGCTT GTGCCAAAGG ATCACACCAC CACCTTCGCT GTCAGATCCA 
CTTAAAGAGC TCTTTCGGCA ACAGGAAGTA GTAAGGATGA AACTGCGTTT GCAACATAGT 
ATTGAAAGGG AAAAACTTAT TGTATCCAAT GAACAAGAAG TTCTGCGAGT TCATTACAGA 
GCTGCAAGAA CACTGGCCAA TCAAACACTG CCATTTAGTG CTTGTACTGT CTTACTGGAT 
GCAGAAGTGT ACAATGTGCC CTTGGACTCA CAGTCTGATG ACAGCAAAAC TTCTGTGAGA 
GATCGATTTA ATGCAAGACA GTTCATGTCC TGGTTGCAAG ATGTGGACGA TAAATTTGAC 
AAGCTAAAGA CCTGTCTCTT AATGAGGCAG CAACACGAAG CTGCAGCTTT AAATGCCGTC 
CAGAGGCTAG AATGGCAGCT CAAACTCCAG GAGCTGGACC CTGCCACGTA TAAGTCTCTC 
AGTATTTATG AAATCCAGGA GTTTTATGTT CCCCTTGTTG ATGTTAATGA TGATTTTGAA 
CTGACTCCTA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd12 ( XM_006523399.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523462.1 Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X6, mRNA.

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CloneID	OMu52957
Clone ID Related Accession (Same CDS sequence)	XM_006523399.4 , XM_006523399.3
Accession Version	XM_006523399.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin repeat domain-containing protein 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523399.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGAAAAA 
GAAGGTCCAG AGAAGAAGAA AACAAAAAAA GAAGCTGGAA CTAAGAAGTC CACCCCAGTT 
AGCATTCTTT TTGGTTATCC ACTTTCTGAA CGAAAGCAGA TGGCACTCCT AATGCAGATG 
ACAGCAAGGG ACAACAGCCC AGATTCCACA CCAAGTCATC CGTCACAGGC AACGCCAGCC 
CAGAAGAAAA CGCCCAGCTC TTCATCTCGA CAGAAAGATA AAATTAATAA AAGAAATGAG 
CGTGGCGAAA CTCCTTTACA CATGGCCGCT ATTCGAGGAG ACGTGAAGCA AGTCAAAGAG 
TTAATTAGTT TAGGGGCAAA CGTCAATGTG AAGGACTTTG CAGGTTGGAC CCCTTTGCAT 
GAAGCTTGCA ATGTTGGCTA TTACGATGTT GCTAAGATAC TGATAGCAGC TGGCGCAGAC 
GTTAACACAC AAGGACTGGA TGATGACACC CCGCTCCATG ACTCTGCCAG CAGTGGCCAT 
AGAGACATAG TGAAGTTGTT GCTTCGTCAT GGCGGGAATC CATTCCAAGC TAATAAACAT 
GGAGAACGGC CAGTGGATGT GGCAGAGACA GAGGAGTTGG AGTTGCTGCT AAAAAGAGAG 
GTGCCTTTGT CTGGCGATGA TGAAAGTTAT ACAGATTCAG AAGAGGCTCA GTCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCA GAGACAGAGA AAGACTCTTT CGTCTGTGGC 
AGTAAAATCC TCCCAAGCAA AGCCCCGCTG CCTTCTGCAC TGGATGAGTA TGAGTTCAAA 
GATGATGATG AAGAAGAGAT AAGTAAGATG ATTGACGACA GGCACATTCT TAGGAAAGAA 
CAGCGGAGAG AGAATGAATC TGAAGCAGAA AGGAGTGGTC TGTTTGCAAA ACAGGAGAAA 
ACCTTCTATT CTAAGTCATT TAAAACAAAA AAACAGAAGC CGTCAAGGGT TCTGTGCTCC 
AGTACTGAAA GCTCTGATGA AGAGGGTCTT CAGAACAGAA AGATTTCTAC TGCTTGTTCT 
GTTGCTGAAT CATCACATCC TGATATGCAA GCCAAAAAAG AATATGAATA CAAACAGAAA 
GGCAAAGTTA AAAGAAAATT AAAAAATCAG AATAAAAACA AAGAGAACCA AGAGCTGAAA 
CAAGAAAAAG AGGGGAAAGA AAATAGCAGG ATAGCAACCT TGACAGTTAA TACTGCGCTG 
GATTGTTCAG AAAAATACAG AGAGGAGGGG AACTTCAGGA AATCTTTTAG CCCAAAGGAT 
GATACTTCAT TACACTTATT TCATATTTCC ACTGGTAAAT CTCCCAAACA TTCCTGTGGA 
TTAAGTGAAA AACAGACACC ACTGAAGCAA GAACACACGA AAGCATGCAT ATCCCCAGGA 
AGTTCTGAAA TGTCATTACA GCCTGATCTC ACTCGGTATG ATAATGCAGA ACCTGAAAGT 
TCAAATGTCA AATCTTATAA GCACAAGGAA AAAAACAAAC AGCAGAAAGA TTTTCACATA 
GAGTTTGGTG AAAAATCAAA TGCCAAAATT AAAGATGAAG ATCATAATCC AGCATTTGAA 
AACTCAGACT GCACACTGAA GAAGATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAA 
TTAAAACATA AAGAGAAGGA TAAAGAAAAG CACAAAAAGG AAGTTGAAGG TGAAAAGGAA 
AAATATAAAA ATAAAGACAA TGCTAAAGAG CTACAGAGGA GCATCGAATT TGACAGAGAG 
TTTTGGAAAG AAAATTTTTT TAAAAGTGAT GAAACTGAGG ATCTCTTTTT AACTATGGAA 
CATGAGTCCA TAATAGAAAA AAAGACAAAA ATGGAAAAAA ACATGAAAGA TGATAGGTCA 
GCTAAAGAAA AGCATGTCTC AAAAGAAAGG AACTTTAAAG AAGAACGGGA AAAGACTAAA 
AAGGAAAATG AAAAATCTCT TAGGGAAGAA AAGGTAAAAG ATCCGAAAGA AGAAAAAGAC 
AGTGTATCCA CAGAGAAGGA GGCCGAGTGC TGTTCGGTGG GTGTCAGTGT GAGCCAGGAA 
GCTGCAGGGC TGCATTCATC AGAAAAGGAA ATGGATGCTG AGAAGCAAGA AAGACACACA 
AAAGAGAGGG AAAAGTCTGA TCGGCGCTTT CAGACTAAGG AGAAAGAGCT TGACAGGAAC 
GAGAGGAAAT TTTCTGAAAA AGAAAAGAAG ATAAAACATG AACATAAGTC AGAAAGAGAG 
AAGCTGGATT TCAGTGACTG CACTGACAGA GTGAGAGAGA AGGACAAGCT GTACTCACAC 
CAGACAGAAA GGTGCCACAA GGACAGCGAG AAGCTGAAAA ACACTGTCAG AAAGACTGAT 
GACAGAGAGA GGAACAGAGA AAAGGCGGAT AGAAAGCACG ACAGAGAGAA ACCTGAACGA 
GAGAGGTACT TAGCAGAAAA CAAAGAAAAG CACTTAGAGA AAAGAAAGCA ATCTGACAGC 
AGTGAGTATT CCAAATCAGA GAAGGTCAGA ACTAAAGACA GAGACAGGGA AGGAGAGAAG 
AAGGAGAAAC TTAGAGATAA AGAAAGTGTA AATGTAACTA ACTTGAAACA TTTGCAGGAA 
GAGAGAAAGT CCAGTACAGG AGACAGCAGT GGTAAAACCC AGCATGAGAA AGCTCTGTCC 
CTTAAAGAAA GGGCAAGAGA TGAGCCTTTG AAAACTCCAG ATGGAAAAGA GAAAGATAAA 
AAGGATATAG ACAGATACAA AGAGCGAGAC AAGCGTAAAG ATAAAGCCCA GCTAAATACT 
TTAATCAGAC TAAAATCTGA AACAGAGAAG CTTAAACCTA AATTATCACC AGCATCAAAA 
GATACTCGAC CTAAAGAAAA GAGGTTAGTA AATGATGATT TAATGCAGAC AAGTTTTGAA 
CGAATGCTTA GCCTTAAAGA CCTAGAAATA GAACAGTGGC ACAGAAAACA TAAGGAAAAA 
ATTAAGCAAA AGGAAAAAGA ACGATTACGC AATCGTACTT GTTTAGAACT TAAAGTAAAA 
GATAAAGAAA AAACAAAACA TGCACCCACA GAATCCAAAA GTAAAGAACT TACCAGGTCA 
AGAAGTTCAG AGTTGACTGA TGTTTATAGC AAGGAGAAAC AATCTAAAGA TGTTGGCAGT 
AATCGCTCAC AATCTGTTGA CAACAGAAAT GTATTGAATT TAGGAAAGCC ATCCTACATT 
TCAGAGAGTG GCTCCAACCG GTCTCCTAGG TCGGAAGGTG AGAGGCTGGG TCTTAGCTCT 
AGGTCTGTGT CCATGCTTTC TGTTGCTAGT TCAGAAGACT CTTGCCATAC AGTGACAACC 
CCAAGGCCTC TCGTTGAGTA TGACTCTGAC TTTATGTTAG AGGGCTCGGA ATCTCAGTTG 
TCATTTTCTC AGTCACCTTT TTTGCCAAAT GCCAAATCTC CAGCTCTTTA TGAAAGAGAG 
CTGGAGGGTC TCACTGAACT ACCAGACCGA CTTAAACCAC CATATACAAA CAGACTTCCA 
GTGTGCCATC TCAGGTCATC TTCTGTGGAA GATGTTAAAC TAATTACTAA TGAGGTAAGA 
CCTGCTGTAG AAATTAGAAG ATGCAGTATG CCCTCAGTTA TCTGTGAGCC CATAAAACAT 
TTCCAAGTAT CAGAAGAAAG CAGTCAAGGC AACTTAGCTG TTCCCAGAGA GACCTGTCTG 
TCTCCCAAGC CTGAAGTGTC TTCAGCAGTA CCTGAGAGGG GCCTTTCGCA TGTGTCTGAC 
TTACATCCTA ACTGTACAGG CTCTCCAACG AGGTCTGCAA ACAGCAGCTA TATTTCATCT 
GATGTAAATG TAATCAAGAG TGCAACTCTT GTGGGTACAC TGATTGACAG TTCGATGCAT 
TTGGAACCGT CTAATCAGGT TGGAGTGATT CCAAGTAAAT CATGGGAGAC GCCTGTTGAT 
AAACTGGAGT CTTTAAGCAC CAATGACTTC ATCTGTCCAG ATTCTAATAT ATCTGATCAA 
GATTCCTCTG CTCAGAGTTT TTATAATTCA GAAAACAAAA TGTTAAGAGA ACAAAATACT 
GACTGCTTAC CTCCGCACCA GCCTGAACTG CCAGGAAACT CATGTGCTCA GGACCCAGCA 
TCCTTTCTGT CTTCACCTCA GCCCTGCTCT TTTTCCAACC AACCACTGGC AGGTGCTGAC 
TCTGTTCCGA AACCCGTGTC TTTGTCATAT GTTGTCAGTC AAGAGCCAGG TGTTTTACAA 
CAGAAAAGTG CAGCTCAGAT GGCCAGTTCT GTTTTAGATA GTGATAATAA CTTTCCAGAG 
GATGTGGAAA ATACATTTGT CTTAGCAGAC ATTCAGAAGA CAAATTCTTT TTTACCAGCT 
TACTCTGAAA GCACTGTGCA AGAAGCACTC CCAAGCTTTG AGAAGGCCAA TACTTTGCTT 
GTGTTGCCAT CAGAAAAGGA CTTCAATGGG AGTGATACTG CTCAGCCAAA CACACATTAT 
GCATTTAGCA AACTCACATA CAAGTCTTCC AATAGCCAGG AAATTGAGAA GGGCCCAGAT 
GATACTCAAA TCATTTCACA TGAAAAGGAA AACAAATTAC AAAGTTTGGT CTTAACTCAG 
TTGAGTAAAT GTGATTCTGA TTTGTATGAG ATGAATGCAG GGCTGCCAAA AGGAAACCTG 
AATGAACAGG ATAACCCAAA GCATGGTCTT GACAGCGAAA AGTACTCGCT TTCCACTGAA 
GATGAAGAAT CTCAGCAGAG CACCTTATCA AGTGTGGAAA ACCATTCACA GCAGTCGGCC 
CAACCAGAGA TGCATAAATA TGACCAGCTT AGTAAAGTAG AACTAGAAGA AAATGCCGAA 
GACGATAAAA CTGAAAACCA GACCCCTCAA AGAGTGACTA GAAACAAAGC AAGCACAGTG 
GTCAATCCAA GCAAGCCCAT GCTTGCTGGC TGTGCCCTTG TAGCAGAGAA AGATAACGAG 
TCCTCCCCTA GGGGACGAAT CAGACTCACT GAAGAGGATG ATCCTCAGAT CCACCATCCT 
CGGAAGAGGA AAGTGTCACG GGTTCCTCAG CCTGTGCAAG TGAGCCCCTC TTTACTGCAA 
GCAAAGGAGA AAACTCAGCA GTCTCTAGCA GCCATTGTAG ATTCTCTGAA GTTAGATGAG 
ATTGAGCCAT ACAGTTCTGA GAGAGCAAAT CCATATTTTG AATATTTGCA CATAAGAAAA 
AAAATTGAAG AAAAACGCAA GTTGCTATGT AGCGTTATCC CTCAAGCTCC TCAGTATTAT 
GATGAATACG TAACGTTTAA TGGATCCTAT CTCCTGGATG GAAACCCCTT AAGCAAGATT 
TGTATTCCTA CAATCACACC ACCACCTTCG CTGTCAGATC CACTTAAAGA GCTCTTTCGG 
CAACAGGAAG TAGTAAGGAT GAAACTGCGT TTGCAACATA GTATTGAAAG GGAAAAACTT 
ATTGTATCCA ATGAACAAGA AGTTCTGCGA GTTCATTACA GAGCTGCAAG AACACTGGCC 
AATCAAACAC TGCCATTTAG TGCTTGTACT GTCTTACTGG ATGCAGAAGT GTACAATGTG 
CCCTTGGACT CACAGTCTGA TGACAGCAAA ACTTCTGTGA GAGATCGATT TAATGCAAGA 
CAGTTCATGT CCTGGTTGCA AGATGTGGAC GATAAATTTG ACAAGCTAAA GACCTGTCTC 
TTAATGAGGC AGCAACACGA AGCTGCAGCT TTAAATGCCG TCCAGAGGCT AGAATGGCAG 
CTCAAACTCC AGGAGCTGGA CCCTGCCACG TATAAGTCTC TCAGTATTTA TGAAATCCAG 
GAGTTTTATG TTCCCCTTGT TGATGTTAAT GATGATTTTG AACTGACTCC TATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523462.1
CDS	258..6314
Translation	MPKSGFTKPVQSENSDSDSSMVERPYGRKSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSGDDESYTDSEEAQSVNPSSVDENIDSETEKDSFVCGSKILPSKAPLPSALDEYEFKDDDEEEISKMIDDRHILRKEQRRENESEAERSGLFAKQEKTFYSKSFKTKKQKPSRVLCSSTESSDEEGLQNRKISTACSVAESSHPDMQAKKEYEYKQKGKVKRKLKNQNKNKENQELKQEKEGKENSRIATLTVNTALDCSEKYREEGNFRKSFSPKDDTSLHLFHISTGKSPKHSCGLSEKQTPLKQEHTKACISPGSSEMSLQPDLTRYDNAEPESSNVKSYKHKEKNKQQKDFHIEFGEKSNAKIKDEDHNPAFENSDCTLKKMDKEGKTLKKHKLKHKEKDKEKHKKEVEGEKEKYKNKDNAKELQRSIEFDREFWKENFFKSDETEDLFLTMEHESIIEKKTKMEKNMKDDRSAKEKHVSKERNFKEEREKTKKENEKSLREEKVKDPKEEKDSVSTEKEAECCSVGVSVSQEAAGLHSSEKEMDAEKQERHTKEREKSDRRFQTKEKELDRNERKFSEKEKKIKHEHKSEREKLDFSDCTDRVREKDKLYSHQTERCHKDSEKLKNTVRKTDDRERNREKADRKHDREKPERERYLAENKEKHLEKRKQSDSSEYSKSEKVRTKDRDREGEKKEKLRDKESVNVTNLKHLQEERKSSTGDSSGKTQHEKALSLKERARDEPLKTPDGKEKDKKDIDRYKERDKRKDKAQLNTLIRLKSETEKLKPKLSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRTCLELKVKDKEKTKHAPTESKSKELTRSRSSELTDVYSKEKQSKDVGSNRSQSVDNRNVLNLGKPSYISESGSNRSPRSEGERLGLSSRSVSMLSVASSEDSCHTVTTPRPLVEYDSDFMLEGSESQLSFSQSPFLPNAKSPALYERELEGLTELPDRLKPPYTNRLPVCHLRSSSVEDVKLITNEVRPAVEIRRCSMPSVICEPIKHFQVSEESSQGNLAVPRETCLSPKPEVSSAVPERGLSHVSDLHPNCTGSPTRSANSSYISSDVNVIKSATLVGTLIDSSMHLEPSNQVGVIPSKSWETPVDKLESLSTNDFICPDSNISDQDSSAQSFYNSENKMLREQNTDCLPPHQPELPGNSCAQDPASFLSSPQPCSFSNQPLAGADSVPKPVSLSYVVSQEPGVLQQKSAAQMASSVLDSDNNFPEDVENTFVLADIQKTNSFLPAYSESTVQEALPSFEKANTLLVLPSEKDFNGSDTAQPNTHYAFSKLTYKSSNSQEIEKGPDDTQIISHEKENKLQSLVLTQLSKCDSDLYEMNAGLPKGNLNEQDNPKHGLDSEKYSLSTEDEESQQSTLSSVENHSQQSAQPEMHKYDQLSKVELEENAEDDKTENQTPQRVTRNKASTVVNPSKPMLAGCALVAEKDNESSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIEPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSLSIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_006523399.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat domain 12 (Ankrd12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523399.3

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAGT 
ATGGTGGAGA GGCCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCTTA CAGCAAAACT 
TCAAAAATTG ACCGAGGTGA TATGGGCAAG GAAATGAGAG AGAAACCATC CATGAAACGA 
AAGCTGCCTT TTACAGTCAG CCCATCGAGA AATGAGGAGC GCGACTCAGA CACAGAAAAA 
GAAGGTCCAG AGAAGAAGAA AACAAAAAAA GAAGCTGGAA CTAAGAAGTC CACCCCAGTT 
AGCATTCTTT TTGGTTATCC ACTTTCTGAA CGAAAGCAGA TGGCACTCCT AATGCAGATG 
ACAGCAAGGG ACAACAGCCC AGATTCCACA CCAAGTCATC CGTCACAGGC AACGCCAGCC 
CAGAAGAAAA CGCCCAGCTC TTCATCTCGA CAGAAAGATA AAATTAATAA AAGAAATGAG 
CGTGGCGAAA CTCCTTTACA CATGGCCGCT ATTCGAGGAG ACGTGAAGCA AGTCAAAGAG 
TTAATTAGTT TAGGGGCAAA CGTCAATGTG AAGGACTTTG CAGGTTGGAC CCCTTTGCAT 
GAAGCTTGCA ATGTTGGCTA TTACGATGTT GCTAAGATAC TGATAGCAGC TGGCGCAGAC 
GTTAACACAC AAGGACTGGA TGATGACACC CCGCTCCATG ACTCTGCCAG CAGTGGCCAT 
AGAGACATAG TGAAGTTGTT GCTTCGTCAT GGCGGGAATC CATTCCAAGC TAATAAACAT 
GGAGAACGGC CAGTGGATGT GGCAGAGACA GAGGAGTTGG AGTTGCTGCT AAAAAGAGAG 
GTGCCTTTGT CTGGCGATGA TGAAAGTTAT ACAGATTCAG AAGAGGCTCA GTCTGTAAAT 
CCTTCTAGTG TTGATGAAAA TATTGACTCA GAGACAGAGA AAGACTCTTT CGTCTGTGGC 
AGTAAAATCC TCCCAAGCAA AGCCCCGCTG CCTTCTGCAC TGGATGAGTA TGAGTTCAAA 
GATGATGATG AAGAAGAGAT AAGTAAGATG ATTGACGACA GGCACATTCT TAGGAAAGAA 
CAGCGGAGAG AGAATGAATC TGAAGCAGAA AGGAGTGGTC TGTTTGCAAA ACAGGAGAAA 
ACCTTCTATT CTAAGTCATT TAAAACAAAA AAACAGAAGC CGTCAAGGGT TCTGTGCTCC 
AGTACTGAAA GCTCTGATGA AGAGGGTCTT CAGAACAGAA AGATTTCTAC TGCTTGTTCT 
GTTGCTGAAT CATCACATCC TGATATGCAA GCCAAAAAAG AATATGAATA CAAACAGAAA 
GGCAAAGTTA AAAGAAAATT AAAAAATCAG AATAAAAACA AAGAGAACCA AGAGCTGAAA 
CAAGAAAAAG AGGGGAAAGA AAATAGCAGG ATAGCAACCT TGACAGTTAA TACTGCGCTG 
GATTGTTCAG AAAAATACAG AGAGGAGGGG AACTTCAGGA AATCTTTTAG CCCAAAGGAT 
GATACTTCAT TACACTTATT TCATATTTCC ACTGGTAAAT CTCCCAAACA TTCCTGTGGA 
TTAAGTGAAA AACAGACACC ACTGAAGCAA GAACACACGA AAGCATGCAT ATCCCCAGGA 
AGTTCTGAAA TGTCATTACA GCCTGATCTC ACTCGGTATG ATAATGCAGA ACCTGAAAGT 
TCAAATGTCA AATCTTATAA GCACAAGGAA AAAAACAAAC AGCAGAAAGA TTTTCACATA 
GAGTTTGGTG AAAAATCAAA TGCCAAAATT AAAGATGAAG ATCATAATCC AGCATTTGAA 
AACTCAGACT GCACACTGAA GAAGATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAA 
TTAAAACATA AAGAGAAGGA TAAAGAAAAG CACAAAAAGG AAGTTGAAGG TGAAAAGGAA 
AAATATAAAA ATAAAGACAA TGCTAAAGAG CTACAGAGGA GCATCGAATT TGACAGAGAG 
TTTTGGAAAG AAAATTTTTT TAAAAGTGAT GAAACTGAGG ATCTCTTTTT AACTATGGAA 
CATGAGTCCA TAATAGAAAA AAAGACAAAA ATGGAAAAAA ACATGAAAGA TGATAGGTCA 
GCTAAAGAAA AGCATGTCTC AAAAGAAAGG AACTTTAAAG AAGAACGGGA AAAGACTAAA 
AAGGAAAATG AAAAATCTCT TAGGGAAGAA AAGGTAAAAG ATCCGAAAGA AGAAAAAGAC 
AGTGTATCCA CAGAGAAGGA GGCCGAGTGC TGTTCGGTGG GTGTCAGTGT GAGCCAGGAA 
GCTGCAGGGC TGCATTCATC AGAAAAGGAA ATGGATGCTG AGAAGCAAGA AAGACACACA 
AAAGAGAGGG AAAAGTCTGA TCGGCGCTTT CAGACTAAGG AGAAAGAGCT TGACAGGAAC 
GAGAGGAAAT TTTCTGAAAA AGAAAAGAAG ATAAAACATG AACATAAGTC AGAAAGAGAG 
AAGCTGGATT TCAGTGACTG CACTGACAGA GTGAGAGAGA AGGACAAGCT GTACTCACAC 
CAGACAGAAA GGTGCCACAA GGACAGCGAG AAGCTGAAAA ACACTGTCAG AAAGACTGAT 
GACAGAGAGA GGAACAGAGA AAAGGCGGAT AGAAAGCACG ACAGAGAGAA ACCTGAACGA 
GAGAGGTACT TAGCAGAAAA CAAAGAAAAG CACTTAGAGA AAAGAAAGCA ATCTGACAGC 
AGTGAGTATT CCAAATCAGA GAAGGTCAGA ACTAAAGACA GAGACAGGGA AGGAGAGAAG 
AAGGAGAAAC TTAGAGATAA AGAAAGTGTA AATGTAACTA ACTTGAAACA TTTGCAGGAA 
GAGAGAAAGT CCAGTACAGG AGACAGCAGT GGTAAAACCC AGCATGAGAA AGCTCTGTCC 
CTTAAAGAAA GGGCAAGAGA TGAGCCTTTG AAAACTCCAG ATGGAAAAGA GAAAGATAAA 
AAGGATATAG ACAGATACAA AGAGCGAGAC AAGCGTAAAG ATAAAGCCCA GCTAAATACT 
TTAATCAGAC TAAAATCTGA AACAGAGAAG CTTAAACCTA AATTATCACC AGCATCAAAA 
GATACTCGAC CTAAAGAAAA GAGGTTAGTA AATGATGATT TAATGCAGAC AAGTTTTGAA 
CGAATGCTTA GCCTTAAAGA CCTAGAAATA GAACAGTGGC ACAGAAAACA TAAGGAAAAA 
ATTAAGCAAA AGGAAAAAGA ACGATTACGC AATCGTACTT GTTTAGAACT TAAAGTAAAA 
GATAAAGAAA AAACAAAACA TGCACCCACA GAATCCAAAA GTAAAGAACT TACCAGGTCA 
AGAAGTTCAG AGTTGACTGA TGTTTATAGC AAGGAGAAAC AATCTAAAGA TGTTGGCAGT 
AATCGCTCAC AATCTGTTGA CAACAGAAAT GTATTGAATT TAGGAAAGCC ATCCTACATT 
TCAGAGAGTG GCTCCAACCG GTCTCCTAGG TCGGAAGGTG AGAGGCTGGG TCTTAGCTCT 
AGGTCTGTGT CCATGCTTTC TGTTGCTAGT TCAGAAGACT CTTGCCATAC AGTGACAACC 
CCAAGGCCTC TCGTTGAGTA TGACTCTGAC TTTATGTTAG AGGGCTCGGA ATCTCAGTTG 
TCATTTTCTC AGTCACCTTT TTTGCCAAAT GCCAAATCTC CAGCTCTTTA TGAAAGAGAG 
CTGGAGGGTC TCACTGAACT ACCAGACCGA CTTAAACCAC CATATACAAA CAGACTTCCA 
GTGTGCCATC TCAGGTCATC TTCTGTGGAA GATGTTAAAC TAATTACTAA TGAGGTAAGA 
CCTGCTGTAG AAATTAGAAG ATGCAGTATG CCCTCAGTTA TCTGTGAGCC CATAAAACAT 
TTCCAAGTAT CAGAAGAAAG CAGTCAAGGC AACTTAGCTG TTCCCAGAGA GACCTGTCTG 
TCTCCCAAGC CTGAAGTGTC TTCAGCAGTA CCTGAGAGGG GCCTTTCGCA TGTGTCTGAC 
TTACATCCTA ACTGTACAGG CTCTCCAACG AGGTCTGCAA ACAGCAGCTA TATTTCATCT 
GATGTAAATG TAATCAAGAG TGCAACTCTT GTGGGTACAC TGATTGACAG TTCGATGCAT 
TTGGAACCGT CTAATCAGGT TGGAGTGATT CCAAGTAAAT CATGGGAGAC GCCTGTTGAT 
AAACTGGAGT CTTTAAGCAC CAATGACTTC ATCTGTCCAG ATTCTAATAT ATCTGATCAA 
GATTCCTCTG CTCAGAGTTT TTATAATTCA GAAAACAAAA TGTTAAGAGA ACAAAATACT 
GACTGCTTAC CTCCGCACCA GCCTGAACTG CCAGGAAACT CATGTGCTCA GGACCCAGCA 
TCCTTTCTGT CTTCACCTCA GCCCTGCTCT TTTTCCAACC AACCACTGGC AGGTGCTGAC 
TCTGTTCCGA AACCCGTGTC TTTGTCATAT GTTGTCAGTC AAGAGCCAGG TGTTTTACAA 
CAGAAAAGTG CAGCTCAGAT GGCCAGTTCT GTTTTAGATA GTGATAATAA CTTTCCAGAG 
GATGTGGAAA ATACATTTGT CTTAGCAGAC ATTCAGAAGA CAAATTCTTT TTTACCAGCT 
TACTCTGAAA GCACTGTGCA AGAAGCACTC CCAAGCTTTG AGAAGGCCAA TACTTTGCTT 
GTGTTGCCAT CAGAAAAGGA CTTCAATGGG AGTGATACTG CTCAGCCAAA CACACATTAT 
GCATTTAGCA AACTCACATA CAAGTCTTCC AATAGCCAGG AAATTGAGAA GGGCCCAGAT 
GATACTCAAA TCATTTCACA TGAAAAGGAA AACAAATTAC AAAGTTTGGT CTTAACTCAG 
TTGAGTAAAT GTGATTCTGA TTTGTATGAG ATGAATGCAG GGCTGCCAAA AGGAAACCTG 
AATGAACAGG ATAACCCAAA GCATGGTCTT GACAGCGAAA AGTACTCGCT TTCCACTGAA 
GATGAAGAAT CTCAGCAGAG CACCTTATCA AGTGTGGAAA ACCATTCACA GCAGTCGGCC 
CAACCAGAGA TGCATAAATA TGACCAGCTT AGTAAAGTAG AACTAGAAGA AAATGCCGAA 
GACGATAAAA CTGAAAACCA GACCCCTCAA AGAGTGACTA GAAACAAAGC AAGCACAGTG 
GTCAATCCAA GCAAGCCCAT GCTTGCTGGC TGTGCCCTTG TAGCAGAGAA AGATAACGAG 
TCCTCCCCTA GGGGACGAAT CAGACTCACT GAAGAGGATG ATCCTCAGAT CCACCATCCT 
CGGAAGAGGA AAGTGTCACG GGTTCCTCAG CCTGTGCAAG TGAGCCCCTC TTTACTGCAA 
GCAAAGGAGA AAACTCAGCA GTCTCTAGCA GCCATTGTAG ATTCTCTGAA GTTAGATGAG 
ATTGAGCCAT ACAGTTCTGA GAGAGCAAAT CCATATTTTG AATATTTGCA CATAAGAAAA 
AAAATTGAAG AAAAACGCAA GTTGCTATGT AGCGTTATCC CTCAAGCTCC TCAGTATTAT 
GATGAATACG TAACGTTTAA TGGATCCTAT CTCCTGGATG GAAACCCCTT AAGCAAGATT 
TGTATTCCTA CAATCACACC ACCACCTTCG CTGTCAGATC CACTTAAAGA GCTCTTTCGG 
CAACAGGAAG TAGTAAGGAT GAAACTGCGT TTGCAACATA GTATTGAAAG GGAAAAACTT 
ATTGTATCCA ATGAACAAGA AGTTCTGCGA GTTCATTACA GAGCTGCAAG AACACTGGCC 
AATCAAACAC TGCCATTTAG TGCTTGTACT GTCTTACTGG ATGCAGAAGT GTACAATGTG 
CCCTTGGACT CACAGTCTGA TGACAGCAAA ACTTCTGTGA GAGATCGATT TAATGCAAGA 
CAGTTCATGT CCTGGTTGCA AGATGTGGAC GATAAATTTG ACAAGCTAAA GACCTGTCTC 
TTAATGAGGC AGCAACACGA AGCTGCAGCT TTAAATGCCG TCCAGAGGCT AGAATGGCAG 
CTCAAACTCC AGGAGCTGGA CCCTGCCACG TATAAGTCTC TCAGTATTTA TGAAATCCAG 
GAGTTTTATG TTCCCCTTGT TGATGTTAAT GATGATTTTG AACTGACTCC TATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Ankrd12
Entrez Gene ID	106585
Full Name	ankyrin repeat domain 12
Synonyms	2900001A12Rik,AI447928,ANCO-2,AV347965,GAC-1,mKIAA0874
General protein information	Preferred Names ankyrin repeat domain 12 Names ankyrin repeat domain-containing protein 12
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E1.1

Rattus norvegicus (Norway rat)	Ankrd12	NP_001101708.1
	LOC705942	XP_002808200.1
Homo sapiens (human)	ANKRD12	NP_056023.3
Pan troglodytes (chimpanzee)	ANKRD12	XP_001139573.3
Bos taurus (cattle)	ANKRD12	XP_002697790.1
Gallus gallus (chicken)	ANKRD12	XP_423591.3
Canis lupus familiaris (dog)	ANKRD12	XP_537329.2
Mus musculus (house mouse)	Ankrd12	NP_001020743.1

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Ankrd12 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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