ANKRD12 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015469150.1	XP_015324636.1	ankyrin repeat domain-containing protein 12 isoform X1
XM_024984609.1	XP_024840377.1	ankyrin repeat domain-containing protein 12 isoform X1
XM_002697743.4	XP_002697789.1	ankyrin repeat domain-containing protein 12 isoform X2
XM_015460128.1	XP_015315614.1	ankyrin repeat domain-containing protein 12 isoform X3
XM_002697744.6	XP_002697790.1	ankyrin repeat domain-containing protein 12 isoform X1
XM_002703009.5	XP_002703055.1	ankyrin repeat domain-containing protein 12 isoform X1
XM_015469151.1	XP_015324637.1	ankyrin repeat domain-containing protein 12 isoform X2
XM_002697744.5	XP_002697790.1	ankyrin repeat domain-containing protein 12 isoform X1
XM_015469152.1	XP_015324638.1	ankyrin repeat domain-containing protein 12 isoform X3
XM_024984610.1	XP_024840378.1	ankyrin repeat domain-containing protein 12 isoform X2
XM_002697743.5	XP_002697789.1	ankyrin repeat domain-containing protein 12 isoform X2
XM_015460128.2	XP_015315614.1	ankyrin repeat domain-containing protein 12 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa88343	XM_015460128.2 Latest version!	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa88343	XM_015469152.1 Latest version!	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa11572	XM_024984609.1 Latest version!	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa11572	XM_002697744.6 Latest version!	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa11572	XM_002703009.5 Latest version!	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa11572	XM_015469150.1 Latest version!	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa11573	XM_002697743.5 Latest version!	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa11573	XM_015469151.1 Latest version!	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa11573	XM_024984610.1 Latest version!	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OBa11572	XM_002697744.5	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa11573	XM_002697743.4	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa88343	XM_015460128.1	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

ANKRD12 ( XM_015460128.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315614.1 Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa88343
Clone ID Related Accession (Same CDS sequence)	XM_015460128.1 , XM_015469152.1 , XM_015460128.2
Accession Version	XM_015460128.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGATG ACAGGCATAT TCTTAGGAAA GAACTGCGAA AAGAGAATGA ATCTGAAGTA 
GAAAAGAGTA GTTTATTTGC AAAACAGGAA AAAACCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTTTTGTAT TCAAGTTCTG AAAGTTCAGA TGAAGAAATT 
CTTCAAAACA AAAAGTGTTC TGCTCCATGT TCTGTCCCTG AAACATCAAA TTCTGATATT 
CAAACCAAAA AGGAATATGG AGTTTCAAAT GAACACAAAC AGAAAGGCAA AGTTAAAAGA 
AAATTAAAGA ACCAGAACAA AAATAAAGAG AACCAAGAGC TGAAGCAAGA AAAAGAGGGG 
AAAGAAAATA CCAGAGTAAC AAACTTGACA GTTAATACTG CACTAGATTG TTCAGATAAG 
ACCAGAGATG AGGGGAATTT TAGGAAATCT TTTAGCCCGA AAGATGATAC TTCATTACAT 
TTATTTCATA TTTCCAGTGG TAAATCTCCC AAACATTCTT GTGGACTCAG TGAAAAGCAG 
TCAACACCAC TAAAACAAGA ACATTCTAAA ACCTGTTTAT CACCAGGAAG TTCTGAAATG 
TCATTACAGC CTGATCTTGT TCGGTATGAT AATATAGAAT CTGAATTCTT GCCAGAAAGT 
TCAAGTGTAA AATCTTGTAA GCATAAGGAA AAAAACAAAC ATCATAAAGA TTTCCACTTA 
GAATTTGGTG AAAAGTCAAA TGCCAAAATA AAAGATGATG ATCACAGTCC AACATTTGAA 
AATTCAGATT GCACACTGAA AAAAATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAG 
TTAAAACATA AAGAAAGGGA AAAAGAAAAG CATAAAAAAG AAATTGAAGG TGAAAAGGAA 
AAATACAAAA GTAGGGATGG TACTAAAGAG CTGCAACGGA GTGTGGAATT TGATAGAGAG 
TTTTGGAAAG AGAATTTTTT TAAAAGTGAT GAAACCGAAG ATCTATTTTT AAGTATGGAA 
CATGAGTCCC TAACGTTAGA AAAAAAGTCA AAGTTGGAGA AAAACATAAA AGATGATAAA 
TCAACTAAGG AAAAGCATAT CTCCAAAGAG AGAAACTTTA AAGAAGAACG GGAAAAGATT 
AAAAAGGAAA GTGAGAAATC TTTTAGGGAG GAAAAAATAA AAGATTTAAA AGATGACAGA 
GAGAACGTAC TTACAGATAA AGAAACAGAA TTCAGCTCTT TAGGTGTAAG TGCCACTGAA 
GAGACTATAG GGTTACATTC AGTGGAAAAG GAAATAGAAA TTGAAAAGCA TATGAAAGAA 
AGTAAGGAAA AATCTGAGAA ACGATTTCAA ACTAAAGATA AGGACATTGA GAGGGCTGAC 
AGAAAAAATT CTGAAAAAGA GAAGAAGATA AAACATGAGC ATAAGTCAGA GAAAGACAGA 
TTAGATCCTA GTGAATGTGT CGACAGAATA AAAGAAAAGG ACAAGCTATA TTCACATCAC 
ACAGAAAAAT GCCATAGAGA AGGTGAGAAG CTAAAAAACA TTACTACCAT TAAAAAAAAC 
GATGACAGAG ATAAAAGTAG AGAAAAGATG GATAGGAAAC ATGACAAAGA AAAACCTGAG 
AAGGAGAGAC ATCCAGTAGA GAGCAAAGAG AAGCACTTGA TGGAAAAAAA AAACAAGCCA 
TCAGATAATA GTGACTATAC TAAATCAGAA AAAAGCAAAA ATAAAGAAAA AGATAGGGAG 
GTAGATAAAA AGGACAAATC TAGAGACAGT GTAAATATAA GTAATTCCAA ACACTTCCAG 
GAAGAGAGAA AGTCAAGTAT AACAGACAGT AATAAAGCAC AACATGAAAA AACTCTCTCT 
CTCAAAGAAA AAACAAAAGA TGAACCTTTG AGAACTCCTG ATGGAAAAGA AAAAGATAAA 
AAAGATATAG ATAGATATAA AGAACGAGAC AAACATAAGG ATAAAATTCT GAGTAGTTCA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCGAAGT CATCACCAGC ATCAAAAGAT 
ACTCGGCCCA AAGAAAAGAG GCTTGTGAAT GACGACTTAA TGCAGACCAG CTTTGAACGG 
ATGCTGAGCC TTAAAGACTT GGAAATAGAG CAGTGGCACA GAAAACACAA GGAAAAGATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAT CGCAGTTGTT TAGAACTTAA AGTAAAAGAT 
AAAGAAAAAA CAAAGCATGC ACTAGCTGAG TCCAAAAATA AAGAACTTAC TAGATCAAAG 
AGTTCTGAGT TGACTGATAC TTACATTAAG GAGAAACACT GTAAAGATGC TGTAAGTAAT 
AGATCCCAAT CTGTGGACAC CAAAAATACG ATGAGTTTAG GCAAGTCATC CTTTGTTTCA 
GATAATAGCT TGAACAGGTC TCCTAGATCA GAAAGTGAAA AGCCAGGTCT CAGCTCCAGG 
TCTGTCTCCT TGATTTCAGT GGCTAGCTCA GAAGATTCCT GCCTTACTAC AATGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCAGAC TTTATGTTAG AGGGTTCGGA TTCCCAAATG 
TCCTTTTCCC AGTCACCGTT TTTGCCAGTT GCCAAATCTC CTGCCCTTCA TGACAGGGAG 
CTGGATAGCC TGACTGATCT ATCAGAGCGG ATTAAAGCAT CGTATGCTAG CAGGCTTCTG 
ACCTCCCATC TCCGGGCATC TTCTGTCGAA GATGCTAAAC TAGTTACAAA TGAAGGGAGA 
TCAGCTGCAG AAGCCCGAAG ATGTAGCATG CCCTCTGTCA TTTGTGAGCA TACAAAGCAA 
TTCCAAACAA TATCAGAAGA GGGCAGTCAA GATGGCTCAG CTGTGCCAAG AGAGATTTGT 
CCTTCTCCCA AATCTGAGGT ATCCTCCAAC GTGCCTGAAA GAGAACTTTT GAGCGTGTCC 
AACATACATT CCAGTTTTGC AGCCTCTCCA ACTAGATCTA TAAACAGCAA ATATAATTCT 
ACTGATACAA ATGTCATCAA GGGCACTGCT CCCTTGGGCA TGCTGATGGG CAGTCCTGTG 
CACTTAGAGT CCTCTAATCA GGTTGGTGTG ATCCAAAGTA AGTCATGGGA GATGGAGTCG 
TTAAACACCG GAGACTTTAG CTGCCCAAAT TCTGCTGCAC CTGATCAAGA TTCTTCTGTT 
CAGGGTTTTT GTAATCCTGA AAACAAAGTA TTGAGAGAAC AGAATATTGA TTTTGTGTCC 
ATGCACCAGA CTGAATTGCC GGCAAACACT TGTGCTCAGG ATCCAGCGTC CGTCCTTCCT 
TCCCAGCAAC CTTGCTCTTC GCATAGCCAA TCACTTTCAG ATGCTGAATC TATTCCCAGA 
CATACTTCTT TGTCCTATGT CACCACTCAA GAACCAGGTA TTCTACAGCA GAAAAATGCA 
ATTCAAATGA TCAGTTCTGT TTTGGATACT GATACTGAAC CTACAAAAGA TATGGAAAAT 
ACTTTTGTCC TAACAGACGT TCAGAAGACA GATGCCTTTG TCCCAGTGTA CTCTGAAAGC 
ACTGTTGAAG AAGCAGCACC AAATTTTGAG AAAGCTAGTA CTTTGCCAGT ATTGCCATCA 
GAAAAGGACT TTAATGGAAG TGACGGGTCT TCCCAGCCAA ATCCTCATTA TGCATTTAGC 
AAGCTAATGT TTAAGTCTTC CAGTGGCCAT GAGGCTGAAA AAAGCACTTC TGATACTCAG 
GATACTTCAC ATGAAAAGGA AAACAAACTG GAGAGTTTGG TTTTGACTCA CTTAAATGAT 
AAGTGTGATT CTGATTTATG TGAAATGAAT GTAGGGGTGC CAAAAGGAAA CCTGAGTGAA 
CCAGATAATC CAAAACATTG TCCTGAAAGT GAGAAGTGTT TGCTTTCCAT CGAGGATGAG 
GAGTCACAAC AAAGTACTTT ATCGAGTCTG GAGAACCATC CACAGCCATC GGCTCCACCA 
GAAATGCATA AATACGGACA CTTAGTTAAA GTAGAACTAG AGGAAAGTGC TGAAGATGAT 
AAACCTGAAA GCCAGATCCC TCAAAGGATG ACCAGAAACA AAGGAAACAC AGGAGCCAGT 
CAAAGCAAGC AGCTTCTGGC CAGCTGTGCA CTGTTGCCAG ACAAAGACAG TGAGTCTGCG 
TCCCCTCGAG GAAGAATAAG ATTAACTGAG GAGGACGACC CTCAGATTCA CCACCCGCGG 
AAAAGGAAAG TGTCCCGGGT GCCTCAGCCG GTGCAAGTGA GTCCTTCTCT GCTGCAAGCA 
AAAGAGAAAA CCCAGCAGTC CCTGGCCGCC ATTGTAGACT CGCTGAAGCT CGATGAGATT 
CAGCCATACA GCTCAGAGCG CGCAAATCCC TATTTTGAAT ACTTGCACAT AAGAAAAAAG 
ATTGAAGAAA AGCGCAAGTT GTTATGTAGT GTGATCCCTC AGGCACCTCA GTATTATGAT 
GAATACGTGA CGTTTAATGG GTCGTATCTC CTGGACGGAA ACCCCTTAAG CAAGATCTGC 
ATACCCACAA TTACACCCCC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TCAGGATGAA ACTACGTTTG CAGCACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAATG AACAGGAGGT TCTTCGGGTT CATTACAGAG CTGCAAGGAC ACTGGCAAAT 
CAGACACTGC CATTCAGCGC ATGCACGGTT CTCCTGGATG CGGAAGTGTA CAGCGTGCCC 
CTGGATGCCC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAG 
TTCATGTCAT GGTTACAAGA TGTGGATGAT AAATTCGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AGCATGAAGC TGCAGCTTTA AATGCCGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATCTATGA AATTCAGGAG 
TTTTACGTTC CGCTGGTTGA TGTTAACGAT GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315614.1
CDS	3920..8953
Translation	MIDDRHILRKELRKENESEVEKSSLFAKQEKTFYPKSFKSKKQKPSRVLYSSSESSDEEILQNKKCSAPCSVPETSNSDIQTKKEYGVSNEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRVTNLTVNTALDCSDKTRDEGNFRKSFSPKDDTSLHLFHISSGKSPKHSCGLSEKQSTPLKQEHSKTCLSPGSSEMSLQPDLVRYDNIESEFLPESSSVKSCKHKEKNKHHKDFHLEFGEKSNAKIKDDDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKSRDGTKELQRSVEFDREFWKENFFKSDETEDLFLSMEHESLTLEKKSKLEKNIKDDKSTKEKHISKERNFKEEREKIKKESEKSFREEKIKDLKDDRENVLTDKETEFSSLGVSATEETIGLHSVEKEIEIEKHMKESKEKSEKRFQTKDKDIERADRKNSEKEKKIKHEHKSEKDRLDPSECVDRIKEKDKLYSHHTEKCHREGEKLKNITTIKKNDDRDKSREKMDRKHDKEKPEKERHPVESKEKHLMEKKNKPSDNSDYTKSEKSKNKEKDREVDKKDKSRDSVNISNSKHFQEERKSSITDSNKAQHEKTLSLKEKTKDEPLRTPDGKEKDKKDIDRYKERDKHKDKILSSSLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRSCLELKVKDKEKTKHALAESKNKELTRSKSSELTDTYIKEKHCKDAVSNRSQSVDTKNTMSLGKSSFVSDNSLNRSPRSESEKPGLSSRSVSLISVASSEDSCLTTMTTPRPPVEYDSDFMLEGSDSQMSFSQSPFLPVAKSPALHDRELDSLTDLSERIKASYASRLLTSHLRASSVEDAKLVTNEGRSAAEARRCSMPSVICEHTKQFQTISEEGSQDGSAVPREICPSPKSEVSSNVPERELLSVSNIHSSFAASPTRSINSKYNSTDTNVIKGTAPLGMLMGSPVHLESSNQVGVIQSKSWEMESLNTGDFSCPNSAAPDQDSSVQGFCNPENKVLREQNIDFVSMHQTELPANTCAQDPASVLPSQQPCSSHSQSLSDAESIPRHTSLSYVTTQEPGILQQKNAIQMISSVLDTDTEPTKDMENTFVLTDVQKTDAFVPVYSESTVEEAAPNFEKASTLPVLPSEKDFNGSDGSSQPNPHYAFSKLMFKSSSGHEAEKSTSDTQDTSHEKENKLESLVLTHLNDKCDSDLCEMNVGVPKGNLSEPDNPKHCPESEKCLLSIEDEESQQSTLSSLENHPQPSAPPEMHKYGHLVKVELEESAEDDKPESQIPQRMTRNKGNTGASQSKQLLASCALLPDKDSESASPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYSVPLDAQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_015460128.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460128.1

ORF Insert Sequence:

ATGATTGATG ACAGGCATAT TCTTAGGAAA GAACTGCGAA AAGAGAATGA ATCTGAAGTA 
GAAAAGAGTA GTTTATTTGC AAAACAGGAA AAAACCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTTTTGTAT TCAAGTTCTG AAAGTTCAGA TGAAGAAATT 
CTTCAAAACA AAAAGTGTTC TGCTCCATGT TCTGTCCCTG AAACATCAAA TTCTGATATT 
CAAACCAAAA AGGAATATGG AGTTTCAAAT GAACACAAAC AGAAAGGCAA AGTTAAAAGA 
AAATTAAAGA ACCAGAACAA AAATAAAGAG AACCAAGAGC TGAAGCAAGA AAAAGAGGGG 
AAAGAAAATA CCAGAGTAAC AAACTTGACA GTTAATACTG CACTAGATTG TTCAGATAAG 
ACCAGAGATG AGGGGAATTT TAGGAAATCT TTTAGCCCGA AAGATGATAC TTCATTACAT 
TTATTTCATA TTTCCAGTGG TAAATCTCCC AAACATTCTT GTGGACTCAG TGAAAAGCAG 
TCAACACCAC TAAAACAAGA ACATTCTAAA ACCTGTTTAT CACCAGGAAG TTCTGAAATG 
TCATTACAGC CTGATCTTGT TCGGTATGAT AATATAGAAT CTGAATTCTT GCCAGAAAGT 
TCAAGTGTAA AATCTTGTAA GCATAAGGAA AAAAACAAAC ATCATAAAGA TTTCCACTTA 
GAATTTGGTG AAAAGTCAAA TGCCAAAATA AAAGATGATG ATCACAGTCC AACATTTGAA 
AATTCAGATT GCACACTGAA AAAAATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAG 
TTAAAACATA AAGAAAGGGA AAAAGAAAAG CATAAAAAAG AAATTGAAGG TGAAAAGGAA 
AAATACAAAA GTAGGGATGG TACTAAAGAG CTGCAACGGA GTGTGGAATT TGATAGAGAG 
TTTTGGAAAG AGAATTTTTT TAAAAGTGAT GAAACCGAAG ATCTATTTTT AAGTATGGAA 
CATGAGTCCC TAACGTTAGA AAAAAAGTCA AAGTTGGAGA AAAACATAAA AGATGATAAA 
TCAACTAAGG AAAAGCATAT CTCCAAAGAG AGAAACTTTA AAGAAGAACG GGAAAAGATT 
AAAAAGGAAA GTGAGAAATC TTTTAGGGAG GAAAAAATAA AAGATTTAAA AGATGACAGA 
GAGAACGTAC TTACAGATAA AGAAACAGAA TTCAGCTCTT TAGGTGTAAG TGCCACTGAA 
GAGACTATAG GGTTACATTC AGTGGAAAAG GAAATAGAAA TTGAAAAGCA TATGAAAGAA 
AGTAAGGAAA AATCTGAGAA ACGATTTCAA ACTAAAGATA AGGACATTGA GAGGGCTGAC 
AGAAAAAATT CTGAAAAAGA GAAGAAGATA AAACATGAGC ATAAGTCAGA GAAAGACAGA 
TTAGATCCTA GTGAATGTGT CGACAGAATA AAAGAAAAGG ACAAGCTATA TTCACATCAC 
ACAGAAAAAT GCCATAGAGA AGGTGAGAAG CTAAAAAACA TTACTACCAT TAAAAAAAAC 
GATGACAGAG ATAAAAGTAG AGAAAAGATG GATAGGAAAC ATGACAAAGA AAAACCTGAG 
AAGGAGAGAC ATCCAGTAGA GAGCAAAGAG AAGCACTTGA TGGAAAAAAA AAACAAGCCA 
TCAGATAATA GTGACTATAC TAAATCAGAA AAAAGCAAAA ATAAAGAAAA AGATAGGGAG 
GTAGATAAAA AGGACAAATC TAGAGACAGT GTAAATATAA GTAATTCCAA ACACTTCCAG 
GAAGAGAGAA AGTCAAGTAT AACAGACAGT AATAAAGCAC AACATGAAAA AACTCTCTCT 
CTCAAAGAAA AAACAAAAGA TGAACCTTTG AGAACTCCTG ATGGAAAAGA AAAAGATAAA 
AAAGATATAG ATAGATATAA AGAACGAGAC AAACATAAGG ATAAAATTCT GAGTAGTTCA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCGAAGT CATCACCAGC ATCAAAAGAT 
ACTCGGCCCA AAGAAAAGAG GCTTGTGAAT GACGACTTAA TGCAGACCAG CTTTGAACGG 
ATGCTGAGCC TTAAAGACTT GGAAATAGAG CAGTGGCACA GAAAACACAA GGAAAAGATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAT CGCAGTTGTT TAGAACTTAA AGTAAAAGAT 
AAAGAAAAAA CAAAGCATGC ACTAGCTGAG TCCAAAAATA AAGAACTTAC TAGATCAAAG 
AGTTCTGAGT TGACTGATAC TTACATTAAG GAGAAACACT GTAAAGATGC TGTAAGTAAT 
AGATCCCAAT CTGTGGACAC CAAAAATACG ATGAGTTTAG GCAAGTCATC CTTTGTTTCA 
GATAATAGCT TGAACAGGTC TCCTAGATCA GAAAGTGAAA AGCCAGGTCT CAGCTCCAGG 
TCTGTCTCCT TGATTTCAGT GGCTAGCTCA GAAGATTCCT GCCTTACTAC AATGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCAGAC TTTATGTTAG AGGGTTCGGA TTCCCAAATG 
TCCTTTTCCC AGTCACCGTT TTTGCCAGTT GCCAAATCTC CTGCCCTTCA TGACAGGGAG 
CTGGATAGCC TGACTGATCT ATCAGAGCGG ATTAAAGCAT CGTATGCTAG CAGGCTTCTG 
ACCTCCCATC TCCGGGCATC TTCTGTCGAA GATGCTAAAC TAGTTACAAA TGAAGGGAGA 
TCAGCTGCAG AAGCCCGAAG ATGTAGCATG CCCTCTGTCA TTTGTGAGCA TACAAAGCAA 
TTCCAAACAA TATCAGAAGA GGGCAGTCAA GATGGCTCAG CTGTGCCAAG AGAGATTTGT 
CCTTCTCCCA AATCTGAGGT ATCCTCCAAC GTGCCTGAAA GAGAACTTTT GAGCGTGTCC 
AACATACATT CCAGTTTTGC AGCCTCTCCA ACTAGATCTA TAAACAGCAA ATATAATTCT 
ACTGATACAA ATGTCATCAA GGGCACTGCT CCCTTGGGCA TGCTGATGGG CAGTCCTGTG 
CACTTAGAGT CCTCTAATCA GGTTGGTGTG ATCCAAAGTA AGTCATGGGA GATGGAGTCG 
TTAAACACCG GAGACTTTAG CTGCCCAAAT TCTGCTGCAC CTGATCAAGA TTCTTCTGTT 
CAGGGTTTTT GTAATCCTGA AAACAAAGTA TTGAGAGAAC AGAATATTGA TTTTGTGTCC 
ATGCACCAGA CTGAATTGCC GGCAAACACT TGTGCTCAGG ATCCAGCGTC CGTCCTTCCT 
TCCCAGCAAC CTTGCTCTTC GCATAGCCAA TCACTTTCAG ATGCTGAATC TATTCCCAGA 
CATACTTCTT TGTCCTATGT CACCACTCAA GAACCAGGTA TTCTACAGCA GAAAAATGCA 
ATTCAAATGA TCAGTTCTGT TTTGGATACT GATACTGAAC CTACAAAAGA TATGGAAAAT 
ACTTTTGTCC TAACAGACGT TCAGAAGACA GATGCCTTTG TCCCAGTGTA CTCTGAAAGC 
ACTGTTGAAG AAGCAGCACC AAATTTTGAG AAAGCTAGTA CTTTGCCAGT ATTGCCATCA 
GAAAAGGACT TTAATGGAAG TGACGGGTCT TCCCAGCCAA ATCCTCATTA TGCATTTAGC 
AAGCTAATGT TTAAGTCTTC CAGTGGCCAT GAGGCTGAAA AAAGCACTTC TGATACTCAG 
GATACTTCAC ATGAAAAGGA AAACAAACTG GAGAGTTTGG TTTTGACTCA CTTAAATGAT 
AAGTGTGATT CTGATTTATG TGAAATGAAT GTAGGGGTGC CAAAAGGAAA CCTGAGTGAA 
CCAGATAATC CAAAACATTG TCCTGAAAGT GAGAAGTGTT TGCTTTCCAT CGAGGATGAG 
GAGTCACAAC AAAGTACTTT ATCGAGTCTG GAGAACCATC CACAGCCATC GGCTCCACCA 
GAAATGCATA AATACGGACA CTTAGTTAAA GTAGAACTAG AGGAAAGTGC TGAAGATGAT 
AAACCTGAAA GCCAGATCCC TCAAAGGATG ACCAGAAACA AAGGAAACAC AGGAGCCAGT 
CAAAGCAAGC AGCTTCTGGC CAGCTGTGCA CTGTTGCCAG ACAAAGACAG TGAGTCTGCG 
TCCCCTCGAG GAAGAATAAG ATTAACTGAG GAGGACGACC CTCAGATTCA CCACCCGCGG 
AAAAGGAAAG TGTCCCGGGT GCCTCAGCCG GTGCAAGTGA GTCCTTCTCT GCTGCAAGCA 
AAAGAGAAAA CCCAGCAGTC CCTGGCCGCC ATTGTAGACT CGCTGAAGCT CGATGAGATT 
CAGCCATACA GCTCAGAGCG CGCAAATCCC TATTTTGAAT ACTTGCACAT AAGAAAAAAG 
ATTGAAGAAA AGCGCAAGTT GTTATGTAGT GTGATCCCTC AGGCACCTCA GTATTATGAT 
GAATACGTGA CGTTTAATGG GTCGTATCTC CTGGACGGAA ACCCCTTAAG CAAGATCTGC 
ATACCCACAA TTACACCCCC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TCAGGATGAA ACTACGTTTG CAGCACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAATG AACAGGAGGT TCTTCGGGTT CATTACAGAG CTGCAAGGAC ACTGGCAAAT 
CAGACACTGC CATTCAGCGC ATGCACGGTT CTCCTGGATG CGGAAGTGTA CAGCGTGCCC 
CTGGATGCCC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAG 
TTCATGTCAT GGTTACAAGA TGTGGATGAT AAATTCGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AGCATGAAGC TGCAGCTTTA AATGCCGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATCTATGA AATTCAGGAG 
TTTTACGTTC CGCTGGTTGA TGTTAACGAT GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_015469152.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324638.1 Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa88343
Clone ID Related Accession (Same CDS sequence)	XM_015460128.1 , XM_015469152.1 , XM_015460128.2
Accession Version	XM_015469152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGATG ACAGGCATAT TCTTAGGAAA GAACTGCGAA AAGAGAATGA ATCTGAAGTA 
GAAAAGAGTA GTTTATTTGC AAAACAGGAA AAAACCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTTTTGTAT TCAAGTTCTG AAAGTTCAGA TGAAGAAATT 
CTTCAAAACA AAAAGTGTTC TGCTCCATGT TCTGTCCCTG AAACATCAAA TTCTGATATT 
CAAACCAAAA AGGAATATGG AGTTTCAAAT GAACACAAAC AGAAAGGCAA AGTTAAAAGA 
AAATTAAAGA ACCAGAACAA AAATAAAGAG AACCAAGAGC TGAAGCAAGA AAAAGAGGGG 
AAAGAAAATA CCAGAGTAAC AAACTTGACA GTTAATACTG CACTAGATTG TTCAGATAAG 
ACCAGAGATG AGGGGAATTT TAGGAAATCT TTTAGCCCGA AAGATGATAC TTCATTACAT 
TTATTTCATA TTTCCAGTGG TAAATCTCCC AAACATTCTT GTGGACTCAG TGAAAAGCAG 
TCAACACCAC TAAAACAAGA ACATTCTAAA ACCTGTTTAT CACCAGGAAG TTCTGAAATG 
TCATTACAGC CTGATCTTGT TCGGTATGAT AATATAGAAT CTGAATTCTT GCCAGAAAGT 
TCAAGTGTAA AATCTTGTAA GCATAAGGAA AAAAACAAAC ATCATAAAGA TTTCCACTTA 
GAATTTGGTG AAAAGTCAAA TGCCAAAATA AAAGATGATG ATCACAGTCC AACATTTGAA 
AATTCAGATT GCACACTGAA AAAAATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAG 
TTAAAACATA AAGAAAGGGA AAAAGAAAAG CATAAAAAAG AAATTGAAGG TGAAAAGGAA 
AAATACAAAA GTAGGGATGG TACTAAAGAG CTGCAACGGA GTGTGGAATT TGATAGAGAG 
TTTTGGAAAG AGAATTTTTT TAAAAGTGAT GAAACCGAAG ATCTATTTTT AAGTATGGAA 
CATGAGTCCC TAACGTTAGA AAAAAAGTCA AAGTTGGAGA AAAACATAAA AGATGATAAA 
TCAACTAAGG AAAAGCATAT CTCCAAAGAG AGAAACTTTA AAGAAGAACG GGAAAAGATT 
AAAAAGGAAA GTGAGAAATC TTTTAGGGAG GAAAAAATAA AAGATTTAAA AGATGACAGA 
GAGAACGTAC TTACAGATAA AGAAACAGAA TTCAGCTCTT TAGGTGTAAG TGCCACTGAA 
GAGACTATAG GGTTACATTC AGTGGAAAAG GAAATAGAAA TTGAAAAGCA TATGAAAGAA 
AGTAAGGAAA AATCTGAGAA ACGATTTCAA ACTAAAGATA AGGACATTGA GAGGGCTGAC 
AGAAAAAATT CTGAAAAAGA GAAGAAGATA AAACATGAGC ATAAGTCAGA GAAAGACAGA 
TTAGATCCTA GTGAATGTGT CGACAGAATA AAAGAAAAGG ACAAGCTATA TTCACATCAC 
ACAGAAAAAT GCCATAGAGA AGGTGAGAAG CTAAAAAACA TTACTACCAT TAAAAAAAAC 
GATGACAGAG ATAAAAGTAG AGAAAAGATG GATAGGAAAC ATGACAAAGA AAAACCTGAG 
AAGGAGAGAC ATCCAGTAGA GAGCAAAGAG AAGCACTTGA TGGAAAAAAA AAACAAGCCA 
TCAGATAATA GTGACTATAC TAAATCAGAA AAAAGCAAAA ATAAAGAAAA AGATAGGGAG 
GTAGATAAAA AGGACAAATC TAGAGACAGT GTAAATATAA GTAATTCCAA ACACTTCCAG 
GAAGAGAGAA AGTCAAGTAT AACAGACAGT AATAAAGCAC AACATGAAAA AACTCTCTCT 
CTCAAAGAAA AAACAAAAGA TGAACCTTTG AGAACTCCTG ATGGAAAAGA AAAAGATAAA 
AAAGATATAG ATAGATATAA AGAACGAGAC AAACATAAGG ATAAAATTCT GAGTAGTTCA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCGAAGT CATCACCAGC ATCAAAAGAT 
ACTCGGCCCA AAGAAAAGAG GCTTGTGAAT GACGACTTAA TGCAGACCAG CTTTGAACGG 
ATGCTGAGCC TTAAAGACTT GGAAATAGAG CAGTGGCACA GAAAACACAA GGAAAAGATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAT CGCAGTTGTT TAGAACTTAA AGTAAAAGAT 
AAAGAAAAAA CAAAGCATGC ACTAGCTGAG TCCAAAAATA AAGAACTTAC TAGATCAAAG 
AGTTCTGAGT TGACTGATAC TTACATTAAG GAGAAACACT GTAAAGATGC TGTAAGTAAT 
AGATCCCAAT CTGTGGACAC CAAAAATACG ATGAGTTTAG GCAAGTCATC CTTTGTTTCA 
GATAATAGCT TGAACAGGTC TCCTAGATCA GAAAGTGAAA AGCCAGGTCT CAGCTCCAGG 
TCTGTCTCCT TGATTTCAGT GGCTAGCTCA GAAGATTCCT GCCTTACTAC AATGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCAGAC TTTATGTTAG AGGGTTCGGA TTCCCAAATG 
TCCTTTTCCC AGTCACCGTT TTTGCCAGTT GCCAAATCTC CTGCCCTTCA TGACAGGGAG 
CTGGATAGCC TGACTGATCT ATCAGAGCGG ATTAAAGCAT CGTATGCTAG CAGGCTTCTG 
ACCTCCCATC TCCGGGCATC TTCTGTCGAA GATGCTAAAC TAGTTACAAA TGAAGGGAGA 
TCAGCTGCAG AAGCCCGAAG ATGTAGCATG CCCTCTGTCA TTTGTGAGCA TACAAAGCAA 
TTCCAAACAA TATCAGAAGA GGGCAGTCAA GATGGCTCAG CTGTGCCAAG AGAGATTTGT 
CCTTCTCCCA AATCTGAGGT ATCCTCCAAC GTGCCTGAAA GAGAACTTTT GAGCGTGTCC 
AACATACATT CCAGTTTTGC AGCCTCTCCA ACTAGATCTA TAAACAGCAA ATATAATTCT 
ACTGATACAA ATGTCATCAA GGGCACTGCT CCCTTGGGCA TGCTGATGGG CAGTCCTGTG 
CACTTAGAGT CCTCTAATCA GGTTGGTGTG ATCCAAAGTA AGTCATGGGA GATGGAGTCG 
TTAAACACCG GAGACTTTAG CTGCCCAAAT TCTGCTGCAC CTGATCAAGA TTCTTCTGTT 
CAGGGTTTTT GTAATCCTGA AAACAAAGTA TTGAGAGAAC AGAATATTGA TTTTGTGTCC 
ATGCACCAGA CTGAATTGCC GGCAAACACT TGTGCTCAGG ATCCAGCGTC CGTCCTTCCT 
TCCCAGCAAC CTTGCTCTTC GCATAGCCAA TCACTTTCAG ATGCTGAATC TATTCCCAGA 
CATACTTCTT TGTCCTATGT CACCACTCAA GAACCAGGTA TTCTACAGCA GAAAAATGCA 
ATTCAAATGA TCAGTTCTGT TTTGGATACT GATACTGAAC CTACAAAAGA TATGGAAAAT 
ACTTTTGTCC TAACAGACGT TCAGAAGACA GATGCCTTTG TCCCAGTGTA CTCTGAAAGC 
ACTGTTGAAG AAGCAGCACC AAATTTTGAG AAAGCTAGTA CTTTGCCAGT ATTGCCATCA 
GAAAAGGACT TTAATGGAAG TGACGGGTCT TCCCAGCCAA ATCCTCATTA TGCATTTAGC 
AAGCTAATGT TTAAGTCTTC CAGTGGCCAT GAGGCTGAAA AAAGCACTTC TGATACTCAG 
GATACTTCAC ATGAAAAGGA AAACAAACTG GAGAGTTTGG TTTTGACTCA CTTAAATGAT 
AAGTGTGATT CTGATTTATG TGAAATGAAT GTAGGGGTGC CAAAAGGAAA CCTGAGTGAA 
CCAGATAATC CAAAACATTG TCCTGAAAGT GAGAAGTGTT TGCTTTCCAT CGAGGATGAG 
GAGTCACAAC AAAGTACTTT ATCGAGTCTG GAGAACCATC CACAGCCATC GGCTCCACCA 
GAAATGCATA AATACGGACA CTTAGTTAAA GTAGAACTAG AGGAAAGTGC TGAAGATGAT 
AAACCTGAAA GCCAGATCCC TCAAAGGATG ACCAGAAACA AAGGAAACAC AGGAGCCAGT 
CAAAGCAAGC AGCTTCTGGC CAGCTGTGCA CTGTTGCCAG ACAAAGACAG TGAGTCTGCG 
TCCCCTCGAG GAAGAATAAG ATTAACTGAG GAGGACGACC CTCAGATTCA CCACCCGCGG 
AAAAGGAAAG TGTCCCGGGT GCCTCAGCCG GTGCAAGTGA GTCCTTCTCT GCTGCAAGCA 
AAAGAGAAAA CCCAGCAGTC CCTGGCCGCC ATTGTAGACT CGCTGAAGCT CGATGAGATT 
CAGCCATACA GCTCAGAGCG CGCAAATCCC TATTTTGAAT ACTTGCACAT AAGAAAAAAG 
ATTGAAGAAA AGCGCAAGTT GTTATGTAGT GTGATCCCTC AGGCACCTCA GTATTATGAT 
GAATACGTGA CGTTTAATGG GTCGTATCTC CTGGACGGAA ACCCCTTAAG CAAGATCTGC 
ATACCCACAA TTACACCCCC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TCAGGATGAA ACTACGTTTG CAGCACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAATG AACAGGAGGT TCTTCGGGTT CATTACAGAG CTGCAAGGAC ACTGGCAAAT 
CAGACACTGC CATTCAGCGC ATGCACGGTT CTCCTGGATG CGGAAGTGTA CAGCGTGCCC 
CTGGATGCCC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAG 
TTCATGTCAT GGTTACAAGA TGTGGATGAT AAATTCGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AGCATGAAGC TGCAGCTTTA AATGCCGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATCTATGA AATTCAGGAG 
TTTTACGTTC CGCTGGTTGA TGTTAACGAT GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324638.1
CDS	3920..8953
Translation	MIDDRHILRKELRKENESEVEKSSLFAKQEKTFYPKSFKSKKQKPSRVLYSSSESSDEEILQNKKCSAPCSVPETSNSDIQTKKEYGVSNEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRVTNLTVNTALDCSDKTRDEGNFRKSFSPKDDTSLHLFHISSGKSPKHSCGLSEKQSTPLKQEHSKTCLSPGSSEMSLQPDLVRYDNIESEFLPESSSVKSCKHKEKNKHHKDFHLEFGEKSNAKIKDDDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKSRDGTKELQRSVEFDREFWKENFFKSDETEDLFLSMEHESLTLEKKSKLEKNIKDDKSTKEKHISKERNFKEEREKIKKESEKSFREEKIKDLKDDRENVLTDKETEFSSLGVSATEETIGLHSVEKEIEIEKHMKESKEKSEKRFQTKDKDIERADRKNSEKEKKIKHEHKSEKDRLDPSECVDRIKEKDKLYSHHTEKCHREGEKLKNITTIKKNDDRDKSREKMDRKHDKEKPEKERHPVESKEKHLMEKKNKPSDNSDYTKSEKSKNKEKDREVDKKDKSRDSVNISNSKHFQEERKSSITDSNKAQHEKTLSLKEKTKDEPLRTPDGKEKDKKDIDRYKERDKHKDKILSSSLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRSCLELKVKDKEKTKHALAESKNKELTRSKSSELTDTYIKEKHCKDAVSNRSQSVDTKNTMSLGKSSFVSDNSLNRSPRSESEKPGLSSRSVSLISVASSEDSCLTTMTTPRPPVEYDSDFMLEGSDSQMSFSQSPFLPVAKSPALHDRELDSLTDLSERIKASYASRLLTSHLRASSVEDAKLVTNEGRSAAEARRCSMPSVICEHTKQFQTISEEGSQDGSAVPREICPSPKSEVSSNVPERELLSVSNIHSSFAASPTRSINSKYNSTDTNVIKGTAPLGMLMGSPVHLESSNQVGVIQSKSWEMESLNTGDFSCPNSAAPDQDSSVQGFCNPENKVLREQNIDFVSMHQTELPANTCAQDPASVLPSQQPCSSHSQSLSDAESIPRHTSLSYVTTQEPGILQQKNAIQMISSVLDTDTEPTKDMENTFVLTDVQKTDAFVPVYSESTVEEAAPNFEKASTLPVLPSEKDFNGSDGSSQPNPHYAFSKLMFKSSSGHEAEKSTSDTQDTSHEKENKLESLVLTHLNDKCDSDLCEMNVGVPKGNLSEPDNPKHCPESEKCLLSIEDEESQQSTLSSLENHPQPSAPPEMHKYGHLVKVELEESAEDDKPESQIPQRMTRNKGNTGASQSKQLLASCALLPDKDSESASPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYSVPLDAQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_015469152.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469152.1

ORF Insert Sequence:

ATGATTGATG ACAGGCATAT TCTTAGGAAA GAACTGCGAA AAGAGAATGA ATCTGAAGTA 
GAAAAGAGTA GTTTATTTGC AAAACAGGAA AAAACCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTTTTGTAT TCAAGTTCTG AAAGTTCAGA TGAAGAAATT 
CTTCAAAACA AAAAGTGTTC TGCTCCATGT TCTGTCCCTG AAACATCAAA TTCTGATATT 
CAAACCAAAA AGGAATATGG AGTTTCAAAT GAACACAAAC AGAAAGGCAA AGTTAAAAGA 
AAATTAAAGA ACCAGAACAA AAATAAAGAG AACCAAGAGC TGAAGCAAGA AAAAGAGGGG 
AAAGAAAATA CCAGAGTAAC AAACTTGACA GTTAATACTG CACTAGATTG TTCAGATAAG 
ACCAGAGATG AGGGGAATTT TAGGAAATCT TTTAGCCCGA AAGATGATAC TTCATTACAT 
TTATTTCATA TTTCCAGTGG TAAATCTCCC AAACATTCTT GTGGACTCAG TGAAAAGCAG 
TCAACACCAC TAAAACAAGA ACATTCTAAA ACCTGTTTAT CACCAGGAAG TTCTGAAATG 
TCATTACAGC CTGATCTTGT TCGGTATGAT AATATAGAAT CTGAATTCTT GCCAGAAAGT 
TCAAGTGTAA AATCTTGTAA GCATAAGGAA AAAAACAAAC ATCATAAAGA TTTCCACTTA 
GAATTTGGTG AAAAGTCAAA TGCCAAAATA AAAGATGATG ATCACAGTCC AACATTTGAA 
AATTCAGATT GCACACTGAA AAAAATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAG 
TTAAAACATA AAGAAAGGGA AAAAGAAAAG CATAAAAAAG AAATTGAAGG TGAAAAGGAA 
AAATACAAAA GTAGGGATGG TACTAAAGAG CTGCAACGGA GTGTGGAATT TGATAGAGAG 
TTTTGGAAAG AGAATTTTTT TAAAAGTGAT GAAACCGAAG ATCTATTTTT AAGTATGGAA 
CATGAGTCCC TAACGTTAGA AAAAAAGTCA AAGTTGGAGA AAAACATAAA AGATGATAAA 
TCAACTAAGG AAAAGCATAT CTCCAAAGAG AGAAACTTTA AAGAAGAACG GGAAAAGATT 
AAAAAGGAAA GTGAGAAATC TTTTAGGGAG GAAAAAATAA AAGATTTAAA AGATGACAGA 
GAGAACGTAC TTACAGATAA AGAAACAGAA TTCAGCTCTT TAGGTGTAAG TGCCACTGAA 
GAGACTATAG GGTTACATTC AGTGGAAAAG GAAATAGAAA TTGAAAAGCA TATGAAAGAA 
AGTAAGGAAA AATCTGAGAA ACGATTTCAA ACTAAAGATA AGGACATTGA GAGGGCTGAC 
AGAAAAAATT CTGAAAAAGA GAAGAAGATA AAACATGAGC ATAAGTCAGA GAAAGACAGA 
TTAGATCCTA GTGAATGTGT CGACAGAATA AAAGAAAAGG ACAAGCTATA TTCACATCAC 
ACAGAAAAAT GCCATAGAGA AGGTGAGAAG CTAAAAAACA TTACTACCAT TAAAAAAAAC 
GATGACAGAG ATAAAAGTAG AGAAAAGATG GATAGGAAAC ATGACAAAGA AAAACCTGAG 
AAGGAGAGAC ATCCAGTAGA GAGCAAAGAG AAGCACTTGA TGGAAAAAAA AAACAAGCCA 
TCAGATAATA GTGACTATAC TAAATCAGAA AAAAGCAAAA ATAAAGAAAA AGATAGGGAG 
GTAGATAAAA AGGACAAATC TAGAGACAGT GTAAATATAA GTAATTCCAA ACACTTCCAG 
GAAGAGAGAA AGTCAAGTAT AACAGACAGT AATAAAGCAC AACATGAAAA AACTCTCTCT 
CTCAAAGAAA AAACAAAAGA TGAACCTTTG AGAACTCCTG ATGGAAAAGA AAAAGATAAA 
AAAGATATAG ATAGATATAA AGAACGAGAC AAACATAAGG ATAAAATTCT GAGTAGTTCA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCGAAGT CATCACCAGC ATCAAAAGAT 
ACTCGGCCCA AAGAAAAGAG GCTTGTGAAT GACGACTTAA TGCAGACCAG CTTTGAACGG 
ATGCTGAGCC TTAAAGACTT GGAAATAGAG CAGTGGCACA GAAAACACAA GGAAAAGATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAT CGCAGTTGTT TAGAACTTAA AGTAAAAGAT 
AAAGAAAAAA CAAAGCATGC ACTAGCTGAG TCCAAAAATA AAGAACTTAC TAGATCAAAG 
AGTTCTGAGT TGACTGATAC TTACATTAAG GAGAAACACT GTAAAGATGC TGTAAGTAAT 
AGATCCCAAT CTGTGGACAC CAAAAATACG ATGAGTTTAG GCAAGTCATC CTTTGTTTCA 
GATAATAGCT TGAACAGGTC TCCTAGATCA GAAAGTGAAA AGCCAGGTCT CAGCTCCAGG 
TCTGTCTCCT TGATTTCAGT GGCTAGCTCA GAAGATTCCT GCCTTACTAC AATGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCAGAC TTTATGTTAG AGGGTTCGGA TTCCCAAATG 
TCCTTTTCCC AGTCACCGTT TTTGCCAGTT GCCAAATCTC CTGCCCTTCA TGACAGGGAG 
CTGGATAGCC TGACTGATCT ATCAGAGCGG ATTAAAGCAT CGTATGCTAG CAGGCTTCTG 
ACCTCCCATC TCCGGGCATC TTCTGTCGAA GATGCTAAAC TAGTTACAAA TGAAGGGAGA 
TCAGCTGCAG AAGCCCGAAG ATGTAGCATG CCCTCTGTCA TTTGTGAGCA TACAAAGCAA 
TTCCAAACAA TATCAGAAGA GGGCAGTCAA GATGGCTCAG CTGTGCCAAG AGAGATTTGT 
CCTTCTCCCA AATCTGAGGT ATCCTCCAAC GTGCCTGAAA GAGAACTTTT GAGCGTGTCC 
AACATACATT CCAGTTTTGC AGCCTCTCCA ACTAGATCTA TAAACAGCAA ATATAATTCT 
ACTGATACAA ATGTCATCAA GGGCACTGCT CCCTTGGGCA TGCTGATGGG CAGTCCTGTG 
CACTTAGAGT CCTCTAATCA GGTTGGTGTG ATCCAAAGTA AGTCATGGGA GATGGAGTCG 
TTAAACACCG GAGACTTTAG CTGCCCAAAT TCTGCTGCAC CTGATCAAGA TTCTTCTGTT 
CAGGGTTTTT GTAATCCTGA AAACAAAGTA TTGAGAGAAC AGAATATTGA TTTTGTGTCC 
ATGCACCAGA CTGAATTGCC GGCAAACACT TGTGCTCAGG ATCCAGCGTC CGTCCTTCCT 
TCCCAGCAAC CTTGCTCTTC GCATAGCCAA TCACTTTCAG ATGCTGAATC TATTCCCAGA 
CATACTTCTT TGTCCTATGT CACCACTCAA GAACCAGGTA TTCTACAGCA GAAAAATGCA 
ATTCAAATGA TCAGTTCTGT TTTGGATACT GATACTGAAC CTACAAAAGA TATGGAAAAT 
ACTTTTGTCC TAACAGACGT TCAGAAGACA GATGCCTTTG TCCCAGTGTA CTCTGAAAGC 
ACTGTTGAAG AAGCAGCACC AAATTTTGAG AAAGCTAGTA CTTTGCCAGT ATTGCCATCA 
GAAAAGGACT TTAATGGAAG TGACGGGTCT TCCCAGCCAA ATCCTCATTA TGCATTTAGC 
AAGCTAATGT TTAAGTCTTC CAGTGGCCAT GAGGCTGAAA AAAGCACTTC TGATACTCAG 
GATACTTCAC ATGAAAAGGA AAACAAACTG GAGAGTTTGG TTTTGACTCA CTTAAATGAT 
AAGTGTGATT CTGATTTATG TGAAATGAAT GTAGGGGTGC CAAAAGGAAA CCTGAGTGAA 
CCAGATAATC CAAAACATTG TCCTGAAAGT GAGAAGTGTT TGCTTTCCAT CGAGGATGAG 
GAGTCACAAC AAAGTACTTT ATCGAGTCTG GAGAACCATC CACAGCCATC GGCTCCACCA 
GAAATGCATA AATACGGACA CTTAGTTAAA GTAGAACTAG AGGAAAGTGC TGAAGATGAT 
AAACCTGAAA GCCAGATCCC TCAAAGGATG ACCAGAAACA AAGGAAACAC AGGAGCCAGT 
CAAAGCAAGC AGCTTCTGGC CAGCTGTGCA CTGTTGCCAG ACAAAGACAG TGAGTCTGCG 
TCCCCTCGAG GAAGAATAAG ATTAACTGAG GAGGACGACC CTCAGATTCA CCACCCGCGG 
AAAAGGAAAG TGTCCCGGGT GCCTCAGCCG GTGCAAGTGA GTCCTTCTCT GCTGCAAGCA 
AAAGAGAAAA CCCAGCAGTC CCTGGCCGCC ATTGTAGACT CGCTGAAGCT CGATGAGATT 
CAGCCATACA GCTCAGAGCG CGCAAATCCC TATTTTGAAT ACTTGCACAT AAGAAAAAAG 
ATTGAAGAAA AGCGCAAGTT GTTATGTAGT GTGATCCCTC AGGCACCTCA GTATTATGAT 
GAATACGTGA CGTTTAATGG GTCGTATCTC CTGGACGGAA ACCCCTTAAG CAAGATCTGC 
ATACCCACAA TTACACCCCC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TCAGGATGAA ACTACGTTTG CAGCACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAATG AACAGGAGGT TCTTCGGGTT CATTACAGAG CTGCAAGGAC ACTGGCAAAT 
CAGACACTGC CATTCAGCGC ATGCACGGTT CTCCTGGATG CGGAAGTGTA CAGCGTGCCC 
CTGGATGCCC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAG 
TTCATGTCAT GGTTACAAGA TGTGGATGAT AAATTCGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AGCATGAAGC TGCAGCTTTA AATGCCGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATCTATGA AATTCAGGAG 
TTTTACGTTC CGCTGGTTGA TGTTAACGAT GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_015460128.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315614.1 Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X5, mRNA.

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CloneID	OBa88343
Clone ID Related Accession (Same CDS sequence)	XM_015460128.1 , XM_015469152.1 , XM_015460128.2
Accession Version	XM_015460128.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460128.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGATG ACAGGCATAT TCTTAGGAAA GAACTGCGAA AAGAGAATGA ATCTGAAGTA 
GAAAAGAGTA GTTTATTTGC AAAACAGGAA AAAACCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTTTTGTAT TCAAGTTCTG AAAGTTCAGA TGAAGAAATT 
CTTCAAAACA AAAAGTGTTC TGCTCCATGT TCTGTCCCTG AAACATCAAA TTCTGATATT 
CAAACCAAAA AGGAATATGG AGTTTCAAAT GAACACAAAC AGAAAGGCAA AGTTAAAAGA 
AAATTAAAGA ACCAGAACAA AAATAAAGAG AACCAAGAGC TGAAGCAAGA AAAAGAGGGG 
AAAGAAAATA CCAGAGTAAC AAACTTGACA GTTAATACTG CACTAGATTG TTCAGATAAG 
ACCAGAGATG AGGGGAATTT TAGGAAATCT TTTAGCCCGA AAGATGATAC TTCATTACAT 
TTATTTCATA TTTCCAGTGG TAAATCTCCC AAACATTCTT GTGGACTCAG TGAAAAGCAG 
TCAACACCAC TAAAACAAGA ACATTCTAAA ACCTGTTTAT CACCAGGAAG TTCTGAAATG 
TCATTACAGC CTGATCTTGT TCGGTATGAT AATATAGAAT CTGAATTCTT GCCAGAAAGT 
TCAAGTGTAA AATCTTGTAA GCATAAGGAA AAAAACAAAC ATCATAAAGA TTTCCACTTA 
GAATTTGGTG AAAAGTCAAA TGCCAAAATA AAAGATGATG ATCACAGTCC AACATTTGAA 
AATTCAGATT GCACACTGAA AAAAATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAG 
TTAAAACATA AAGAAAGGGA AAAAGAAAAG CATAAAAAAG AAATTGAAGG TGAAAAGGAA 
AAATACAAAA GTAGGGATGG TACTAAAGAG CTGCAACGGA GTGTGGAATT TGATAGAGAG 
TTTTGGAAAG AGAATTTTTT TAAAAGTGAT GAAACCGAAG ATCTATTTTT AAGTATGGAA 
CATGAGTCCC TAACGTTAGA AAAAAAGTCA AAGTTGGAGA AAAACATAAA AGATGATAAA 
TCAACTAAGG AAAAGCATAT CTCCAAAGAG AGAAACTTTA AAGAAGAACG GGAAAAGATT 
AAAAAGGAAA GTGAGAAATC TTTTAGGGAG GAAAAAATAA AAGATTTAAA AGATGACAGA 
GAGAACGTAC TTACAGATAA AGAAACAGAA TTCAGCTCTT TAGGTGTAAG TGCCACTGAA 
GAGACTATAG GGTTACATTC AGTGGAAAAG GAAATAGAAA TTGAAAAGCA TATGAAAGAA 
AGTAAGGAAA AATCTGAGAA ACGATTTCAA ACTAAAGATA AGGACATTGA GAGGGCTGAC 
AGAAAAAATT CTGAAAAAGA GAAGAAGATA AAACATGAGC ATAAGTCAGA GAAAGACAGA 
TTAGATCCTA GTGAATGTGT CGACAGAATA AAAGAAAAGG ACAAGCTATA TTCACATCAC 
ACAGAAAAAT GCCATAGAGA AGGTGAGAAG CTAAAAAACA TTACTACCAT TAAAAAAAAC 
GATGACAGAG ATAAAAGTAG AGAAAAGATG GATAGGAAAC ATGACAAAGA AAAACCTGAG 
AAGGAGAGAC ATCCAGTAGA GAGCAAAGAG AAGCACTTGA TGGAAAAAAA AAACAAGCCA 
TCAGATAATA GTGACTATAC TAAATCAGAA AAAAGCAAAA ATAAAGAAAA AGATAGGGAG 
GTAGATAAAA AGGACAAATC TAGAGACAGT GTAAATATAA GTAATTCCAA ACACTTCCAG 
GAAGAGAGAA AGTCAAGTAT AACAGACAGT AATAAAGCAC AACATGAAAA AACTCTCTCT 
CTCAAAGAAA AAACAAAAGA TGAACCTTTG AGAACTCCTG ATGGAAAAGA AAAAGATAAA 
AAAGATATAG ATAGATATAA AGAACGAGAC AAACATAAGG ATAAAATTCT GAGTAGTTCA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCGAAGT CATCACCAGC ATCAAAAGAT 
ACTCGGCCCA AAGAAAAGAG GCTTGTGAAT GACGACTTAA TGCAGACCAG CTTTGAACGG 
ATGCTGAGCC TTAAAGACTT GGAAATAGAG CAGTGGCACA GAAAACACAA GGAAAAGATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAT CGCAGTTGTT TAGAACTTAA AGTAAAAGAT 
AAAGAAAAAA CAAAGCATGC ACTAGCTGAG TCCAAAAATA AAGAACTTAC TAGATCAAAG 
AGTTCTGAGT TGACTGATAC TTACATTAAG GAGAAACACT GTAAAGATGC TGTAAGTAAT 
AGATCCCAAT CTGTGGACAC CAAAAATACG ATGAGTTTAG GCAAGTCATC CTTTGTTTCA 
GATAATAGCT TGAACAGGTC TCCTAGATCA GAAAGTGAAA AGCCAGGTCT CAGCTCCAGG 
TCTGTCTCCT TGATTTCAGT GGCTAGCTCA GAAGATTCCT GCCTTACTAC AATGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCAGAC TTTATGTTAG AGGGTTCGGA TTCCCAAATG 
TCCTTTTCCC AGTCACCGTT TTTGCCAGTT GCCAAATCTC CTGCCCTTCA TGACAGGGAG 
CTGGATAGCC TGACTGATCT ATCAGAGCGG ATTAAAGCAT CGTATGCTAG CAGGCTTCTG 
ACCTCCCATC TCCGGGCATC TTCTGTCGAA GATGCTAAAC TAGTTACAAA TGAAGGGAGA 
TCAGCTGCAG AAGCCCGAAG ATGTAGCATG CCCTCTGTCA TTTGTGAGCA TACAAAGCAA 
TTCCAAACAA TATCAGAAGA GGGCAGTCAA GATGGCTCAG CTGTGCCAAG AGAGATTTGT 
CCTTCTCCCA AATCTGAGGT ATCCTCCAAC GTGCCTGAAA GAGAACTTTT GAGCGTGTCC 
AACATACATT CCAGTTTTGC AGCCTCTCCA ACTAGATCTA TAAACAGCAA ATATAATTCT 
ACTGATACAA ATGTCATCAA GGGCACTGCT CCCTTGGGCA TGCTGATGGG CAGTCCTGTG 
CACTTAGAGT CCTCTAATCA GGTTGGTGTG ATCCAAAGTA AGTCATGGGA GATGGAGTCG 
TTAAACACCG GAGACTTTAG CTGCCCAAAT TCTGCTGCAC CTGATCAAGA TTCTTCTGTT 
CAGGGTTTTT GTAATCCTGA AAACAAAGTA TTGAGAGAAC AGAATATTGA TTTTGTGTCC 
ATGCACCAGA CTGAATTGCC GGCAAACACT TGTGCTCAGG ATCCAGCGTC CGTCCTTCCT 
TCCCAGCAAC CTTGCTCTTC GCATAGCCAA TCACTTTCAG ATGCTGAATC TATTCCCAGA 
CATACTTCTT TGTCCTATGT CACCACTCAA GAACCAGGTA TTCTACAGCA GAAAAATGCA 
ATTCAAATGA TCAGTTCTGT TTTGGATACT GATACTGAAC CTACAAAAGA TATGGAAAAT 
ACTTTTGTCC TAACAGACGT TCAGAAGACA GATGCCTTTG TCCCAGTGTA CTCTGAAAGC 
ACTGTTGAAG AAGCAGCACC AAATTTTGAG AAAGCTAGTA CTTTGCCAGT ATTGCCATCA 
GAAAAGGACT TTAATGGAAG TGACGGGTCT TCCCAGCCAA ATCCTCATTA TGCATTTAGC 
AAGCTAATGT TTAAGTCTTC CAGTGGCCAT GAGGCTGAAA AAAGCACTTC TGATACTCAG 
GATACTTCAC ATGAAAAGGA AAACAAACTG GAGAGTTTGG TTTTGACTCA CTTAAATGAT 
AAGTGTGATT CTGATTTATG TGAAATGAAT GTAGGGGTGC CAAAAGGAAA CCTGAGTGAA 
CCAGATAATC CAAAACATTG TCCTGAAAGT GAGAAGTGTT TGCTTTCCAT CGAGGATGAG 
GAGTCACAAC AAAGTACTTT ATCGAGTCTG GAGAACCATC CACAGCCATC GGCTCCACCA 
GAAATGCATA AATACGGACA CTTAGTTAAA GTAGAACTAG AGGAAAGTGC TGAAGATGAT 
AAACCTGAAA GCCAGATCCC TCAAAGGATG ACCAGAAACA AAGGAAACAC AGGAGCCAGT 
CAAAGCAAGC AGCTTCTGGC CAGCTGTGCA CTGTTGCCAG ACAAAGACAG TGAGTCTGCG 
TCCCCTCGAG GAAGAATAAG ATTAACTGAG GAGGACGACC CTCAGATTCA CCACCCGCGG 
AAAAGGAAAG TGTCCCGGGT GCCTCAGCCG GTGCAAGTGA GTCCTTCTCT GCTGCAAGCA 
AAAGAGAAAA CCCAGCAGTC CCTGGCCGCC ATTGTAGACT CGCTGAAGCT CGATGAGATT 
CAGCCATACA GCTCAGAGCG CGCAAATCCC TATTTTGAAT ACTTGCACAT AAGAAAAAAG 
ATTGAAGAAA AGCGCAAGTT GTTATGTAGT GTGATCCCTC AGGCACCTCA GTATTATGAT 
GAATACGTGA CGTTTAATGG GTCGTATCTC CTGGACGGAA ACCCCTTAAG CAAGATCTGC 
ATACCCACAA TTACACCCCC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TCAGGATGAA ACTACGTTTG CAGCACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAATG AACAGGAGGT TCTTCGGGTT CATTACAGAG CTGCAAGGAC ACTGGCAAAT 
CAGACACTGC CATTCAGCGC ATGCACGGTT CTCCTGGATG CGGAAGTGTA CAGCGTGCCC 
CTGGATGCCC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAG 
TTCATGTCAT GGTTACAAGA TGTGGATGAT AAATTCGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AGCATGAAGC TGCAGCTTTA AATGCCGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATCTATGA AATTCAGGAG 
TTTTACGTTC CGCTGGTTGA TGTTAACGAT GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315614.1
CDS	750..5783
Translation	MIDDRHILRKELRKENESEVEKSSLFAKQEKTFYPKSFKSKKQKPSRVLYSSSESSDEEILQNKKCSAPCSVPETSNSDIQTKKEYGVSNEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRVTNLTVNTALDCSDKTRDEGNFRKSFSPKDDTSLHLFHISSGKSPKHSCGLSEKQSTPLKQEHSKTCLSPGSSEMSLQPDLVRYDNIESEFLPESSSVKSCKHKEKNKHHKDFHLEFGEKSNAKIKDDDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKSRDGTKELQRSVEFDREFWKENFFKSDETEDLFLSMEHESLTLEKKSKLEKNIKDDKSTKEKHISKERNFKEEREKIKKESEKSFREEKIKDLKDDRENVLTDKETEFSSLGVSATEETIGLHSVEKEIEIEKHMKESKEKSEKRFQTKDKDIERADRKNSEKEKKIKHEHKSEKDRLDPSECVDRIKEKDKLYSHHTEKCHREGEKLKNITTIKKNDDRDKSREKMDRKHDKEKPEKERHPVESKEKHLMEKKNKPSDNSDYTKSEKSKNKEKDREVDKKDKSRDSVNISNSKHFQEERKSSITDSNKAQHEKTLSLKEKTKDEPLRTPDGKEKDKKDIDRYKERDKHKDKILSSSLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRSCLELKVKDKEKTKHALAESKNKELTRSKSSELTDTYIKEKHCKDAVSNRSQSVDTKNTMSLGKSSFVSDNSLNRSPRSESEKPGLSSRSVSLISVASSEDSCLTTMTTPRPPVEYDSDFMLEGSDSQMSFSQSPFLPVAKSPALHDRELDSLTDLSERIKASYASRLLTSHLRASSVEDAKLVTNEGRSAAEARRCSMPSVICEHTKQFQTISEEGSQDGSAVPREICPSPKSEVSSNVPERELLSVSNIHSSFAASPTRSINSKYNSTDTNVIKGTAPLGMLMGSPVHLESSNQVGVIQSKSWEMESLNTGDFSCPNSAAPDQDSSVQGFCNPENKVLREQNIDFVSMHQTELPANTCAQDPASVLPSQQPCSSHSQSLSDAESIPRHTSLSYVTTQEPGILQQKNAIQMISSVLDTDTEPTKDMENTFVLTDVQKTDAFVPVYSESTVEEAAPNFEKASTLPVLPSEKDFNGSDGSSQPNPHYAFSKLMFKSSSGHEAEKSTSDTQDTSHEKENKLESLVLTHLNDKCDSDLCEMNVGVPKGNLSEPDNPKHCPESEKCLLSIEDEESQQSTLSSLENHPQPSAPPEMHKYGHLVKVELEESAEDDKPESQIPQRMTRNKGNTGASQSKQLLASCALLPDKDSESASPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYSVPLDAQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_015460128.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460128.2

ORF Insert Sequence:

ATGATTGATG ACAGGCATAT TCTTAGGAAA GAACTGCGAA AAGAGAATGA ATCTGAAGTA 
GAAAAGAGTA GTTTATTTGC AAAACAGGAA AAAACCTTCT ATCCTAAATC ATTTAAAAGT 
AAAAAACAAA AGCCATCTAG GGTTTTGTAT TCAAGTTCTG AAAGTTCAGA TGAAGAAATT 
CTTCAAAACA AAAAGTGTTC TGCTCCATGT TCTGTCCCTG AAACATCAAA TTCTGATATT 
CAAACCAAAA AGGAATATGG AGTTTCAAAT GAACACAAAC AGAAAGGCAA AGTTAAAAGA 
AAATTAAAGA ACCAGAACAA AAATAAAGAG AACCAAGAGC TGAAGCAAGA AAAAGAGGGG 
AAAGAAAATA CCAGAGTAAC AAACTTGACA GTTAATACTG CACTAGATTG TTCAGATAAG 
ACCAGAGATG AGGGGAATTT TAGGAAATCT TTTAGCCCGA AAGATGATAC TTCATTACAT 
TTATTTCATA TTTCCAGTGG TAAATCTCCC AAACATTCTT GTGGACTCAG TGAAAAGCAG 
TCAACACCAC TAAAACAAGA ACATTCTAAA ACCTGTTTAT CACCAGGAAG TTCTGAAATG 
TCATTACAGC CTGATCTTGT TCGGTATGAT AATATAGAAT CTGAATTCTT GCCAGAAAGT 
TCAAGTGTAA AATCTTGTAA GCATAAGGAA AAAAACAAAC ATCATAAAGA TTTCCACTTA 
GAATTTGGTG AAAAGTCAAA TGCCAAAATA AAAGATGATG ATCACAGTCC AACATTTGAA 
AATTCAGATT GCACACTGAA AAAAATGGAT AAAGAGGGTA AAACATTAAA AAAACATAAG 
TTAAAACATA AAGAAAGGGA AAAAGAAAAG CATAAAAAAG AAATTGAAGG TGAAAAGGAA 
AAATACAAAA GTAGGGATGG TACTAAAGAG CTGCAACGGA GTGTGGAATT TGATAGAGAG 
TTTTGGAAAG AGAATTTTTT TAAAAGTGAT GAAACCGAAG ATCTATTTTT AAGTATGGAA 
CATGAGTCCC TAACGTTAGA AAAAAAGTCA AAGTTGGAGA AAAACATAAA AGATGATAAA 
TCAACTAAGG AAAAGCATAT CTCCAAAGAG AGAAACTTTA AAGAAGAACG GGAAAAGATT 
AAAAAGGAAA GTGAGAAATC TTTTAGGGAG GAAAAAATAA AAGATTTAAA AGATGACAGA 
GAGAACGTAC TTACAGATAA AGAAACAGAA TTCAGCTCTT TAGGTGTAAG TGCCACTGAA 
GAGACTATAG GGTTACATTC AGTGGAAAAG GAAATAGAAA TTGAAAAGCA TATGAAAGAA 
AGTAAGGAAA AATCTGAGAA ACGATTTCAA ACTAAAGATA AGGACATTGA GAGGGCTGAC 
AGAAAAAATT CTGAAAAAGA GAAGAAGATA AAACATGAGC ATAAGTCAGA GAAAGACAGA 
TTAGATCCTA GTGAATGTGT CGACAGAATA AAAGAAAAGG ACAAGCTATA TTCACATCAC 
ACAGAAAAAT GCCATAGAGA AGGTGAGAAG CTAAAAAACA TTACTACCAT TAAAAAAAAC 
GATGACAGAG ATAAAAGTAG AGAAAAGATG GATAGGAAAC ATGACAAAGA AAAACCTGAG 
AAGGAGAGAC ATCCAGTAGA GAGCAAAGAG AAGCACTTGA TGGAAAAAAA AAACAAGCCA 
TCAGATAATA GTGACTATAC TAAATCAGAA AAAAGCAAAA ATAAAGAAAA AGATAGGGAG 
GTAGATAAAA AGGACAAATC TAGAGACAGT GTAAATATAA GTAATTCCAA ACACTTCCAG 
GAAGAGAGAA AGTCAAGTAT AACAGACAGT AATAAAGCAC AACATGAAAA AACTCTCTCT 
CTCAAAGAAA AAACAAAAGA TGAACCTTTG AGAACTCCTG ATGGAAAAGA AAAAGATAAA 
AAAGATATAG ATAGATATAA AGAACGAGAC AAACATAAGG ATAAAATTCT GAGTAGTTCA 
CTCAAACTAA AATCTGAAGC AGATAAGCCT AAACCGAAGT CATCACCAGC ATCAAAAGAT 
ACTCGGCCCA AAGAAAAGAG GCTTGTGAAT GACGACTTAA TGCAGACCAG CTTTGAACGG 
ATGCTGAGCC TTAAAGACTT GGAAATAGAG CAGTGGCACA GAAAACACAA GGAAAAGATT 
AAGCAAAAAG AAAAGGAACG GTTGAGAAAT CGCAGTTGTT TAGAACTTAA AGTAAAAGAT 
AAAGAAAAAA CAAAGCATGC ACTAGCTGAG TCCAAAAATA AAGAACTTAC TAGATCAAAG 
AGTTCTGAGT TGACTGATAC TTACATTAAG GAGAAACACT GTAAAGATGC TGTAAGTAAT 
AGATCCCAAT CTGTGGACAC CAAAAATACG ATGAGTTTAG GCAAGTCATC CTTTGTTTCA 
GATAATAGCT TGAACAGGTC TCCTAGATCA GAAAGTGAAA AGCCAGGTCT CAGCTCCAGG 
TCTGTCTCCT TGATTTCAGT GGCTAGCTCA GAAGATTCCT GCCTTACTAC AATGACAACC 
CCAAGGCCTC CAGTTGAGTA TGACTCAGAC TTTATGTTAG AGGGTTCGGA TTCCCAAATG 
TCCTTTTCCC AGTCACCGTT TTTGCCAGTT GCCAAATCTC CTGCCCTTCA TGACAGGGAG 
CTGGATAGCC TGACTGATCT ATCAGAGCGG ATTAAAGCAT CGTATGCTAG CAGGCTTCTG 
ACCTCCCATC TCCGGGCATC TTCTGTCGAA GATGCTAAAC TAGTTACAAA TGAAGGGAGA 
TCAGCTGCAG AAGCCCGAAG ATGTAGCATG CCCTCTGTCA TTTGTGAGCA TACAAAGCAA 
TTCCAAACAA TATCAGAAGA GGGCAGTCAA GATGGCTCAG CTGTGCCAAG AGAGATTTGT 
CCTTCTCCCA AATCTGAGGT ATCCTCCAAC GTGCCTGAAA GAGAACTTTT GAGCGTGTCC 
AACATACATT CCAGTTTTGC AGCCTCTCCA ACTAGATCTA TAAACAGCAA ATATAATTCT 
ACTGATACAA ATGTCATCAA GGGCACTGCT CCCTTGGGCA TGCTGATGGG CAGTCCTGTG 
CACTTAGAGT CCTCTAATCA GGTTGGTGTG ATCCAAAGTA AGTCATGGGA GATGGAGTCG 
TTAAACACCG GAGACTTTAG CTGCCCAAAT TCTGCTGCAC CTGATCAAGA TTCTTCTGTT 
CAGGGTTTTT GTAATCCTGA AAACAAAGTA TTGAGAGAAC AGAATATTGA TTTTGTGTCC 
ATGCACCAGA CTGAATTGCC GGCAAACACT TGTGCTCAGG ATCCAGCGTC CGTCCTTCCT 
TCCCAGCAAC CTTGCTCTTC GCATAGCCAA TCACTTTCAG ATGCTGAATC TATTCCCAGA 
CATACTTCTT TGTCCTATGT CACCACTCAA GAACCAGGTA TTCTACAGCA GAAAAATGCA 
ATTCAAATGA TCAGTTCTGT TTTGGATACT GATACTGAAC CTACAAAAGA TATGGAAAAT 
ACTTTTGTCC TAACAGACGT TCAGAAGACA GATGCCTTTG TCCCAGTGTA CTCTGAAAGC 
ACTGTTGAAG AAGCAGCACC AAATTTTGAG AAAGCTAGTA CTTTGCCAGT ATTGCCATCA 
GAAAAGGACT TTAATGGAAG TGACGGGTCT TCCCAGCCAA ATCCTCATTA TGCATTTAGC 
AAGCTAATGT TTAAGTCTTC CAGTGGCCAT GAGGCTGAAA AAAGCACTTC TGATACTCAG 
GATACTTCAC ATGAAAAGGA AAACAAACTG GAGAGTTTGG TTTTGACTCA CTTAAATGAT 
AAGTGTGATT CTGATTTATG TGAAATGAAT GTAGGGGTGC CAAAAGGAAA CCTGAGTGAA 
CCAGATAATC CAAAACATTG TCCTGAAAGT GAGAAGTGTT TGCTTTCCAT CGAGGATGAG 
GAGTCACAAC AAAGTACTTT ATCGAGTCTG GAGAACCATC CACAGCCATC GGCTCCACCA 
GAAATGCATA AATACGGACA CTTAGTTAAA GTAGAACTAG AGGAAAGTGC TGAAGATGAT 
AAACCTGAAA GCCAGATCCC TCAAAGGATG ACCAGAAACA AAGGAAACAC AGGAGCCAGT 
CAAAGCAAGC AGCTTCTGGC CAGCTGTGCA CTGTTGCCAG ACAAAGACAG TGAGTCTGCG 
TCCCCTCGAG GAAGAATAAG ATTAACTGAG GAGGACGACC CTCAGATTCA CCACCCGCGG 
AAAAGGAAAG TGTCCCGGGT GCCTCAGCCG GTGCAAGTGA GTCCTTCTCT GCTGCAAGCA 
AAAGAGAAAA CCCAGCAGTC CCTGGCCGCC ATTGTAGACT CGCTGAAGCT CGATGAGATT 
CAGCCATACA GCTCAGAGCG CGCAAATCCC TATTTTGAAT ACTTGCACAT AAGAAAAAAG 
ATTGAAGAAA AGCGCAAGTT GTTATGTAGT GTGATCCCTC AGGCACCTCA GTATTATGAT 
GAATACGTGA CGTTTAATGG GTCGTATCTC CTGGACGGAA ACCCCTTAAG CAAGATCTGC 
ATACCCACAA TTACACCCCC ACCTTCACTG TCAGATCCAC TTAAAGAGCT TTTTCGACAA 
CAGGAAGTTG TCAGGATGAA ACTACGTTTG CAGCACAGTA TTGAAAGGGA AAAACTCATT 
GTATCCAATG AACAGGAGGT TCTTCGGGTT CATTACAGAG CTGCAAGGAC ACTGGCAAAT 
CAGACACTGC CATTCAGCGC ATGCACGGTT CTCCTGGATG CGGAAGTGTA CAGCGTGCCC 
CTGGATGCCC AGTCTGATGA CAGTAAAACT TCTGTGAGGG ATCGCTTTAA TGCAAGACAG 
TTCATGTCAT GGTTACAAGA TGTGGATGAT AAATTCGACA AATTAAAGAC CTGTCTTTTA 
ATGAGGCAAC AGCATGAAGC TGCAGCTTTA AATGCCGTCC AGAGGTTAGA ATGGCAGCTC 
AAACTCCAGG AACTTGATCC TGCCACCTAT AAATCTATCA GCATCTATGA AATTCAGGAG 
TTTTACGTTC CGCTGGTTGA TGTTAACGAT GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_015469150.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324636.1 Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X4, mRNA.

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CloneID	OBa11572
Clone ID Related Accession (Same CDS sequence)	XM_015469150.1 , XM_024984609.1 , XM_002697744.6 , XM_002703009.5 , XM_002697744.5
Accession Version	XM_015469150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA GAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATGC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAA ACAAAAAAGG AAGCTGGAAA TAAGAAGTCC 
ACTCCAGTTA GCATTCTCTT TGGTTACCCA CTCTCAGAGC GAAAGCAGAT GGCACTTCTT 
ATGCAGATGA CAGCACGGGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCGCAAACA 
CCACCAGCCC AAAAGAAAAC TCCCAGTTCC TCATCTCGAC AAAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCAGCTA TTCGAGGAGA TGTAAAACAA 
GTCAAAGAAC TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA CGTGGGATAT TATGATGTTG CTAAGATACT GATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCCGCTAGT 
AGTGGGCACA GAGATATTGT GAAACTGCTG CTTCGTCATG GTGGAAACCC TTTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTAGATGTG GCAGAAACAG AGGAGCTGGA GTTGTTGCTG 
AAAAGAGAGG TGCCTTTGTC TGATGATGAT GAGAGTTACA CAGATTCTGA AGAGGCTCAG 
TCTGTAAATC CTTCCAGCGT TGATGAAAAT ATTGACTCTG AGACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT AGTTCCCAGT AAAGCACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGAAGAGGAA ATAAATAAAA TGATTGATGA CAGGCATATT 
CTTAGGAAAG AACTGCGAAA AGAGAATGAA TCTGAAGTAG AAAAGAGTAG TTTATTTGCA 
AAACAGGAAA AAACCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTTTTGTATT CAAGTTCTGA AAGTTCAGAT GAAGAAATTC TTCAAAACAA AAAGTGTTCT 
GCTCCATGTT CTGTCCCTGA AACATCAAAT TCTGATATTC AAACCAAAAA GGAATATGGA 
GTTTCAAATG AACACAAACA GAAAGGCAAA GTTAAAAGAA AATTAAAGAA CCAGAACAAA 
AATAAAGAGA ACCAAGAGCT GAAGCAAGAA AAAGAGGGGA AAGAAAATAC CAGAGTAACA 
AACTTGACAG TTAATACTGC ACTAGATTGT TCAGATAAGA CCAGAGATGA GGGGAATTTT 
AGGAAATCTT TTAGCCCGAA AGATGATACT TCATTACATT TATTTCATAT TTCCAGTGGT 
AAATCTCCCA AACATTCTTG TGGACTCAGT GAAAAGCAGT CAACACCACT AAAACAAGAA 
CATTCTAAAA CCTGTTTATC ACCAGGAAGT TCTGAAATGT CATTACAGCC TGATCTTGTT 
CGGTATGATA ATATAGAATC TGAATTCTTG CCAGAAAGTT CAAGTGTAAA ATCTTGTAAG 
CATAAGGAAA AAAACAAACA TCATAAAGAT TTCCACTTAG AATTTGGTGA AAAGTCAAAT 
GCCAAAATAA AAGATGATGA TCACAGTCCA ACATTTGAAA ATTCAGATTG CACACTGAAA 
AAAATGGATA AAGAGGGTAA AACATTAAAA AAACATAAGT TAAAACATAA AGAAAGGGAA 
AAAGAAAAGC ATAAAAAAGA AATTGAAGGT GAAAAGGAAA AATACAAAAG TAGGGATGGT 
ACTAAAGAGC TGCAACGGAG TGTGGAATTT GATAGAGAGT TTTGGAAAGA GAATTTTTTT 
AAAAGTGATG AAACCGAAGA TCTATTTTTA AGTATGGAAC ATGAGTCCCT AACGTTAGAA 
AAAAAGTCAA AGTTGGAGAA AAACATAAAA GATGATAAAT CAACTAAGGA AAAGCATATC 
TCCAAAGAGA GAAACTTTAA AGAAGAACGG GAAAAGATTA AAAAGGAAAG TGAGAAATCT 
TTTAGGGAGG AAAAAATAAA AGATTTAAAA GATGACAGAG AGAACGTACT TACAGATAAA 
GAAACAGAAT TCAGCTCTTT AGGTGTAAGT GCCACTGAAG AGACTATAGG GTTACATTCA 
GTGGAAAAGG AAATAGAAAT TGAAAAGCAT ATGAAAGAAA GTAAGGAAAA ATCTGAGAAA 
CGATTTCAAA CTAAAGATAA GGACATTGAG AGGGCTGACA GAAAAAATTC TGAAAAAGAG 
AAGAAGATAA AACATGAGCA TAAGTCAGAG AAAGACAGAT TAGATCCTAG TGAATGTGTC 
GACAGAATAA AAGAAAAGGA CAAGCTATAT TCACATCACA CAGAAAAATG CCATAGAGAA 
GGTGAGAAGC TAAAAAACAT TACTACCATT AAAAAAAACG ATGACAGAGA TAAAAGTAGA 
GAAAAGATGG ATAGGAAACA TGACAAAGAA AAACCTGAGA AGGAGAGACA TCCAGTAGAG 
AGCAAAGAGA AGCACTTGAT GGAAAAAAAA AACAAGCCAT CAGATAATAG TGACTATACT 
AAATCAGAAA AAAGCAAAAA TAAAGAAAAA GATAGGGAGG TAGATAAAAA GGACAAATCT 
AGAGACAGTG TAAATATAAG TAATTCCAAA CACTTCCAGG AAGAGAGAAA GTCAAGTATA 
ACAGACAGTA ATAAAGCACA ACATGAAAAA ACTCTCTCTC TCAAAGAAAA AACAAAAGAT 
GAACCTTTGA GAACTCCTGA TGGAAAAGAA AAAGATAAAA AAGATATAGA TAGATATAAA 
GAACGAGACA AACATAAGGA TAAAATTCTG AGTAGTTCAC TCAAACTAAA ATCTGAAGCA 
GATAAGCCTA AACCGAAGTC ATCACCAGCA TCAAAAGATA CTCGGCCCAA AGAAAAGAGG 
CTTGTGAATG ACGACTTAAT GCAGACCAGC TTTGAACGGA TGCTGAGCCT TAAAGACTTG 
GAAATAGAGC AGTGGCACAG AAAACACAAG GAAAAGATTA AGCAAAAAGA AAAGGAACGG 
TTGAGAAATC GCAGTTGTTT AGAACTTAAA GTAAAAGATA AAGAAAAAAC AAAGCATGCA 
CTAGCTGAGT CCAAAAATAA AGAACTTACT AGATCAAAGA GTTCTGAGTT GACTGATACT 
TACATTAAGG AGAAACACTG TAAAGATGCT GTAAGTAATA GATCCCAATC TGTGGACACC 
AAAAATACGA TGAGTTTAGG CAAGTCATCC TTTGTTTCAG ATAATAGCTT GAACAGGTCT 
CCTAGATCAG AAAGTGAAAA GCCAGGTCTC AGCTCCAGGT CTGTCTCCTT GATTTCAGTG 
GCTAGCTCAG AAGATTCCTG CCTTACTACA ATGACAACCC CAAGGCCTCC AGTTGAGTAT 
GACTCAGACT TTATGTTAGA GGGTTCGGAT TCCCAAATGT CCTTTTCCCA GTCACCGTTT 
TTGCCAGTTG CCAAATCTCC TGCCCTTCAT GACAGGGAGC TGGATAGCCT GACTGATCTA 
TCAGAGCGGA TTAAAGCATC GTATGCTAGC AGGCTTCTGA CCTCCCATCT CCGGGCATCT 
TCTGTCGAAG ATGCTAAACT AGTTACAAAT GAAGGGAGAT CAGCTGCAGA AGCCCGAAGA 
TGTAGCATGC CCTCTGTCAT TTGTGAGCAT ACAAAGCAAT TCCAAACAAT ATCAGAAGAG 
GGCAGTCAAG ATGGCTCAGC TGTGCCAAGA GAGATTTGTC CTTCTCCCAA ATCTGAGGTA 
TCCTCCAACG TGCCTGAAAG AGAACTTTTG AGCGTGTCCA ACATACATTC CAGTTTTGCA 
GCCTCTCCAA CTAGATCTAT AAACAGCAAA TATAATTCTA CTGATACAAA TGTCATCAAG 
GGCACTGCTC CCTTGGGCAT GCTGATGGGC AGTCCTGTGC ACTTAGAGTC CTCTAATCAG 
GTTGGTGTGA TCCAAAGTAA GTCATGGGAG ATGGAGTCGT TAAACACCGG AGACTTTAGC 
TGCCCAAATT CTGCTGCACC TGATCAAGAT TCTTCTGTTC AGGGTTTTTG TAATCCTGAA 
AACAAAGTAT TGAGAGAACA GAATATTGAT TTTGTGTCCA TGCACCAGAC TGAATTGCCG 
GCAAACACTT GTGCTCAGGA TCCAGCGTCC GTCCTTCCTT CCCAGCAACC TTGCTCTTCG 
CATAGCCAAT CACTTTCAGA TGCTGAATCT ATTCCCAGAC ATACTTCTTT GTCCTATGTC 
ACCACTCAAG AACCAGGTAT TCTACAGCAG AAAAATGCAA TTCAAATGAT CAGTTCTGTT 
TTGGATACTG ATACTGAACC TACAAAAGAT ATGGAAAATA CTTTTGTCCT AACAGACGTT 
CAGAAGACAG ATGCCTTTGT CCCAGTGTAC TCTGAAAGCA CTGTTGAAGA AGCAGCACCA 
AATTTTGAGA AAGCTAGTAC TTTGCCAGTA TTGCCATCAG AAAAGGACTT TAATGGAAGT 
GACGGGTCTT CCCAGCCAAA TCCTCATTAT GCATTTAGCA AGCTAATGTT TAAGTCTTCC 
AGTGGCCATG AGGCTGAAAA AAGCACTTCT GATACTCAGG ATACTTCACA TGAAAAGGAA 
AACAAACTGG AGAGTTTGGT TTTGACTCAC TTAAATGATA AGTGTGATTC TGATTTATGT 
GAAATGAATG TAGGGGTGCC AAAAGGAAAC CTGAGTGAAC CAGATAATCC AAAACATTGT 
CCTGAAAGTG AGAAGTGTTT GCTTTCCATC GAGGATGAGG AGTCACAACA AAGTACTTTA 
TCGAGTCTGG AGAACCATCC ACAGCCATCG GCTCCACCAG AAATGCATAA ATACGGACAC 
TTAGTTAAAG TAGAACTAGA GGAAAGTGCT GAAGATGATA AACCTGAAAG CCAGATCCCT 
CAAAGGATGA CCAGAAACAA AGGAAACACA GGAGCCAGTC AAAGCAAGCA GCTTCTGGCC 
AGCTGTGCAC TGTTGCCAGA CAAAGACAGT GAGTCTGCGT CCCCTCGAGG AAGAATAAGA 
TTAACTGAGG AGGACGACCC TCAGATTCAC CACCCGCGGA AAAGGAAAGT GTCCCGGGTG 
CCTCAGCCGG TGCAAGTGAG TCCTTCTCTG CTGCAAGCAA AAGAGAAAAC CCAGCAGTCC 
CTGGCCGCCA TTGTAGACTC GCTGAAGCTC GATGAGATTC AGCCATACAG CTCAGAGCGC 
GCAAATCCCT ATTTTGAATA CTTGCACATA AGAAAAAAGA TTGAAGAAAA GCGCAAGTTG 
TTATGTAGTG TGATCCCTCA GGCACCTCAG TATTATGATG AATACGTGAC GTTTAATGGG 
TCGTATCTCC TGGACGGAAA CCCCTTAAGC AAGATCTGCA TACCCACAAT TACACCCCCA 
CCTTCACTGT CAGATCCACT TAAAGAGCTT TTTCGACAAC AGGAAGTTGT CAGGATGAAA 
CTACGTTTGC AGCACAGTAT TGAAAGGGAA AAACTCATTG TATCCAATGA ACAGGAGGTT 
CTTCGGGTTC ATTACAGAGC TGCAAGGACA CTGGCAAATC AGACACTGCC ATTCAGCGCA 
TGCACGGTTC TCCTGGATGC GGAAGTGTAC AGCGTGCCCC TGGATGCCCA GTCTGATGAC 
AGTAAAACTT CTGTGAGGGA TCGCTTTAAT GCAAGACAGT TCATGTCATG GTTACAAGAT 
GTGGATGATA AATTCGACAA ATTAAAGACC TGTCTTTTAA TGAGGCAACA GCATGAAGCT 
GCAGCTTTAA ATGCCGTCCA GAGGTTAGAA TGGCAGCTCA AACTCCAGGA ACTTGATCCT 
GCCACCTATA AATCTATCAG CATCTATGAA ATTCAGGAGT TTTACGTTCC GCTGGTTGAT 
GTTAACGATG ACTTTGAATT GACTCCTATA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324636.1
CDS	341..6493
Translation	MPKSGFTKPVQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIDRNDVGKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTCSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTPPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQIVPSKAPLPSALDEYEFKDDDEEEINKMIDDRHILRKELRKENESEVEKSSLFAKQEKTFYPKSFKSKKQKPSRVLYSSSESSDEEILQNKKCSAPCSVPETSNSDIQTKKEYGVSNEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRVTNLTVNTALDCSDKTRDEGNFRKSFSPKDDTSLHLFHISSGKSPKHSCGLSEKQSTPLKQEHSKTCLSPGSSEMSLQPDLVRYDNIESEFLPESSSVKSCKHKEKNKHHKDFHLEFGEKSNAKIKDDDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKSRDGTKELQRSVEFDREFWKENFFKSDETEDLFLSMEHESLTLEKKSKLEKNIKDDKSTKEKHISKERNFKEEREKIKKESEKSFREEKIKDLKDDRENVLTDKETEFSSLGVSATEETIGLHSVEKEIEIEKHMKESKEKSEKRFQTKDKDIERADRKNSEKEKKIKHEHKSEKDRLDPSECVDRIKEKDKLYSHHTEKCHREGEKLKNITTIKKNDDRDKSREKMDRKHDKEKPEKERHPVESKEKHLMEKKNKPSDNSDYTKSEKSKNKEKDREVDKKDKSRDSVNISNSKHFQEERKSSITDSNKAQHEKTLSLKEKTKDEPLRTPDGKEKDKKDIDRYKERDKHKDKILSSSLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRSCLELKVKDKEKTKHALAESKNKELTRSKSSELTDTYIKEKHCKDAVSNRSQSVDTKNTMSLGKSSFVSDNSLNRSPRSESEKPGLSSRSVSLISVASSEDSCLTTMTTPRPPVEYDSDFMLEGSDSQMSFSQSPFLPVAKSPALHDRELDSLTDLSERIKASYASRLLTSHLRASSVEDAKLVTNEGRSAAEARRCSMPSVICEHTKQFQTISEEGSQDGSAVPREICPSPKSEVSSNVPERELLSVSNIHSSFAASPTRSINSKYNSTDTNVIKGTAPLGMLMGSPVHLESSNQVGVIQSKSWEMESLNTGDFSCPNSAAPDQDSSVQGFCNPENKVLREQNIDFVSMHQTELPANTCAQDPASVLPSQQPCSSHSQSLSDAESIPRHTSLSYVTTQEPGILQQKNAIQMISSVLDTDTEPTKDMENTFVLTDVQKTDAFVPVYSESTVEEAAPNFEKASTLPVLPSEKDFNGSDGSSQPNPHYAFSKLMFKSSSGHEAEKSTSDTQDTSHEKENKLESLVLTHLNDKCDSDLCEMNVGVPKGNLSEPDNPKHCPESEKCLLSIEDEESQQSTLSSLENHPQPSAPPEMHKYGHLVKVELEESAEDDKPESQIPQRMTRNKGNTGASQSKQLLASCALLPDKDSESASPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYSVPLDAQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_015469150.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469150.1

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA GAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATGC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAA ACAAAAAAGG AAGCTGGAAA TAAGAAGTCC 
ACTCCAGTTA GCATTCTCTT TGGTTACCCA CTCTCAGAGC GAAAGCAGAT GGCACTTCTT 
ATGCAGATGA CAGCACGGGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCGCAAACA 
CCACCAGCCC AAAAGAAAAC TCCCAGTTCC TCATCTCGAC AAAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCAGCTA TTCGAGGAGA TGTAAAACAA 
GTCAAAGAAC TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA CGTGGGATAT TATGATGTTG CTAAGATACT GATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCCGCTAGT 
AGTGGGCACA GAGATATTGT GAAACTGCTG CTTCGTCATG GTGGAAACCC TTTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTAGATGTG GCAGAAACAG AGGAGCTGGA GTTGTTGCTG 
AAAAGAGAGG TGCCTTTGTC TGATGATGAT GAGAGTTACA CAGATTCTGA AGAGGCTCAG 
TCTGTAAATC CTTCCAGCGT TGATGAAAAT ATTGACTCTG AGACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT AGTTCCCAGT AAAGCACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGAAGAGGAA ATAAATAAAA TGATTGATGA CAGGCATATT 
CTTAGGAAAG AACTGCGAAA AGAGAATGAA TCTGAAGTAG AAAAGAGTAG TTTATTTGCA 
AAACAGGAAA AAACCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTTTTGTATT CAAGTTCTGA AAGTTCAGAT GAAGAAATTC TTCAAAACAA AAAGTGTTCT 
GCTCCATGTT CTGTCCCTGA AACATCAAAT TCTGATATTC AAACCAAAAA GGAATATGGA 
GTTTCAAATG AACACAAACA GAAAGGCAAA GTTAAAAGAA AATTAAAGAA CCAGAACAAA 
AATAAAGAGA ACCAAGAGCT GAAGCAAGAA AAAGAGGGGA AAGAAAATAC CAGAGTAACA 
AACTTGACAG TTAATACTGC ACTAGATTGT TCAGATAAGA CCAGAGATGA GGGGAATTTT 
AGGAAATCTT TTAGCCCGAA AGATGATACT TCATTACATT TATTTCATAT TTCCAGTGGT 
AAATCTCCCA AACATTCTTG TGGACTCAGT GAAAAGCAGT CAACACCACT AAAACAAGAA 
CATTCTAAAA CCTGTTTATC ACCAGGAAGT TCTGAAATGT CATTACAGCC TGATCTTGTT 
CGGTATGATA ATATAGAATC TGAATTCTTG CCAGAAAGTT CAAGTGTAAA ATCTTGTAAG 
CATAAGGAAA AAAACAAACA TCATAAAGAT TTCCACTTAG AATTTGGTGA AAAGTCAAAT 
GCCAAAATAA AAGATGATGA TCACAGTCCA ACATTTGAAA ATTCAGATTG CACACTGAAA 
AAAATGGATA AAGAGGGTAA AACATTAAAA AAACATAAGT TAAAACATAA AGAAAGGGAA 
AAAGAAAAGC ATAAAAAAGA AATTGAAGGT GAAAAGGAAA AATACAAAAG TAGGGATGGT 
ACTAAAGAGC TGCAACGGAG TGTGGAATTT GATAGAGAGT TTTGGAAAGA GAATTTTTTT 
AAAAGTGATG AAACCGAAGA TCTATTTTTA AGTATGGAAC ATGAGTCCCT AACGTTAGAA 
AAAAAGTCAA AGTTGGAGAA AAACATAAAA GATGATAAAT CAACTAAGGA AAAGCATATC 
TCCAAAGAGA GAAACTTTAA AGAAGAACGG GAAAAGATTA AAAAGGAAAG TGAGAAATCT 
TTTAGGGAGG AAAAAATAAA AGATTTAAAA GATGACAGAG AGAACGTACT TACAGATAAA 
GAAACAGAAT TCAGCTCTTT AGGTGTAAGT GCCACTGAAG AGACTATAGG GTTACATTCA 
GTGGAAAAGG AAATAGAAAT TGAAAAGCAT ATGAAAGAAA GTAAGGAAAA ATCTGAGAAA 
CGATTTCAAA CTAAAGATAA GGACATTGAG AGGGCTGACA GAAAAAATTC TGAAAAAGAG 
AAGAAGATAA AACATGAGCA TAAGTCAGAG AAAGACAGAT TAGATCCTAG TGAATGTGTC 
GACAGAATAA AAGAAAAGGA CAAGCTATAT TCACATCACA CAGAAAAATG CCATAGAGAA 
GGTGAGAAGC TAAAAAACAT TACTACCATT AAAAAAAACG ATGACAGAGA TAAAAGTAGA 
GAAAAGATGG ATAGGAAACA TGACAAAGAA AAACCTGAGA AGGAGAGACA TCCAGTAGAG 
AGCAAAGAGA AGCACTTGAT GGAAAAAAAA AACAAGCCAT CAGATAATAG TGACTATACT 
AAATCAGAAA AAAGCAAAAA TAAAGAAAAA GATAGGGAGG TAGATAAAAA GGACAAATCT 
AGAGACAGTG TAAATATAAG TAATTCCAAA CACTTCCAGG AAGAGAGAAA GTCAAGTATA 
ACAGACAGTA ATAAAGCACA ACATGAAAAA ACTCTCTCTC TCAAAGAAAA AACAAAAGAT 
GAACCTTTGA GAACTCCTGA TGGAAAAGAA AAAGATAAAA AAGATATAGA TAGATATAAA 
GAACGAGACA AACATAAGGA TAAAATTCTG AGTAGTTCAC TCAAACTAAA ATCTGAAGCA 
GATAAGCCTA AACCGAAGTC ATCACCAGCA TCAAAAGATA CTCGGCCCAA AGAAAAGAGG 
CTTGTGAATG ACGACTTAAT GCAGACCAGC TTTGAACGGA TGCTGAGCCT TAAAGACTTG 
GAAATAGAGC AGTGGCACAG AAAACACAAG GAAAAGATTA AGCAAAAAGA AAAGGAACGG 
TTGAGAAATC GCAGTTGTTT AGAACTTAAA GTAAAAGATA AAGAAAAAAC AAAGCATGCA 
CTAGCTGAGT CCAAAAATAA AGAACTTACT AGATCAAAGA GTTCTGAGTT GACTGATACT 
TACATTAAGG AGAAACACTG TAAAGATGCT GTAAGTAATA GATCCCAATC TGTGGACACC 
AAAAATACGA TGAGTTTAGG CAAGTCATCC TTTGTTTCAG ATAATAGCTT GAACAGGTCT 
CCTAGATCAG AAAGTGAAAA GCCAGGTCTC AGCTCCAGGT CTGTCTCCTT GATTTCAGTG 
GCTAGCTCAG AAGATTCCTG CCTTACTACA ATGACAACCC CAAGGCCTCC AGTTGAGTAT 
GACTCAGACT TTATGTTAGA GGGTTCGGAT TCCCAAATGT CCTTTTCCCA GTCACCGTTT 
TTGCCAGTTG CCAAATCTCC TGCCCTTCAT GACAGGGAGC TGGATAGCCT GACTGATCTA 
TCAGAGCGGA TTAAAGCATC GTATGCTAGC AGGCTTCTGA CCTCCCATCT CCGGGCATCT 
TCTGTCGAAG ATGCTAAACT AGTTACAAAT GAAGGGAGAT CAGCTGCAGA AGCCCGAAGA 
TGTAGCATGC CCTCTGTCAT TTGTGAGCAT ACAAAGCAAT TCCAAACAAT ATCAGAAGAG 
GGCAGTCAAG ATGGCTCAGC TGTGCCAAGA GAGATTTGTC CTTCTCCCAA ATCTGAGGTA 
TCCTCCAACG TGCCTGAAAG AGAACTTTTG AGCGTGTCCA ACATACATTC CAGTTTTGCA 
GCCTCTCCAA CTAGATCTAT AAACAGCAAA TATAATTCTA CTGATACAAA TGTCATCAAG 
GGCACTGCTC CCTTGGGCAT GCTGATGGGC AGTCCTGTGC ACTTAGAGTC CTCTAATCAG 
GTTGGTGTGA TCCAAAGTAA GTCATGGGAG ATGGAGTCGT TAAACACCGG AGACTTTAGC 
TGCCCAAATT CTGCTGCACC TGATCAAGAT TCTTCTGTTC AGGGTTTTTG TAATCCTGAA 
AACAAAGTAT TGAGAGAACA GAATATTGAT TTTGTGTCCA TGCACCAGAC TGAATTGCCG 
GCAAACACTT GTGCTCAGGA TCCAGCGTCC GTCCTTCCTT CCCAGCAACC TTGCTCTTCG 
CATAGCCAAT CACTTTCAGA TGCTGAATCT ATTCCCAGAC ATACTTCTTT GTCCTATGTC 
ACCACTCAAG AACCAGGTAT TCTACAGCAG AAAAATGCAA TTCAAATGAT CAGTTCTGTT 
TTGGATACTG ATACTGAACC TACAAAAGAT ATGGAAAATA CTTTTGTCCT AACAGACGTT 
CAGAAGACAG ATGCCTTTGT CCCAGTGTAC TCTGAAAGCA CTGTTGAAGA AGCAGCACCA 
AATTTTGAGA AAGCTAGTAC TTTGCCAGTA TTGCCATCAG AAAAGGACTT TAATGGAAGT 
GACGGGTCTT CCCAGCCAAA TCCTCATTAT GCATTTAGCA AGCTAATGTT TAAGTCTTCC 
AGTGGCCATG AGGCTGAAAA AAGCACTTCT GATACTCAGG ATACTTCACA TGAAAAGGAA 
AACAAACTGG AGAGTTTGGT TTTGACTCAC TTAAATGATA AGTGTGATTC TGATTTATGT 
GAAATGAATG TAGGGGTGCC AAAAGGAAAC CTGAGTGAAC CAGATAATCC AAAACATTGT 
CCTGAAAGTG AGAAGTGTTT GCTTTCCATC GAGGATGAGG AGTCACAACA AAGTACTTTA 
TCGAGTCTGG AGAACCATCC ACAGCCATCG GCTCCACCAG AAATGCATAA ATACGGACAC 
TTAGTTAAAG TAGAACTAGA GGAAAGTGCT GAAGATGATA AACCTGAAAG CCAGATCCCT 
CAAAGGATGA CCAGAAACAA AGGAAACACA GGAGCCAGTC AAAGCAAGCA GCTTCTGGCC 
AGCTGTGCAC TGTTGCCAGA CAAAGACAGT GAGTCTGCGT CCCCTCGAGG AAGAATAAGA 
TTAACTGAGG AGGACGACCC TCAGATTCAC CACCCGCGGA AAAGGAAAGT GTCCCGGGTG 
CCTCAGCCGG TGCAAGTGAG TCCTTCTCTG CTGCAAGCAA AAGAGAAAAC CCAGCAGTCC 
CTGGCCGCCA TTGTAGACTC GCTGAAGCTC GATGAGATTC AGCCATACAG CTCAGAGCGC 
GCAAATCCCT ATTTTGAATA CTTGCACATA AGAAAAAAGA TTGAAGAAAA GCGCAAGTTG 
TTATGTAGTG TGATCCCTCA GGCACCTCAG TATTATGATG AATACGTGAC GTTTAATGGG 
TCGTATCTCC TGGACGGAAA CCCCTTAAGC AAGATCTGCA TACCCACAAT TACACCCCCA 
CCTTCACTGT CAGATCCACT TAAAGAGCTT TTTCGACAAC AGGAAGTTGT CAGGATGAAA 
CTACGTTTGC AGCACAGTAT TGAAAGGGAA AAACTCATTG TATCCAATGA ACAGGAGGTT 
CTTCGGGTTC ATTACAGAGC TGCAAGGACA CTGGCAAATC AGACACTGCC ATTCAGCGCA 
TGCACGGTTC TCCTGGATGC GGAAGTGTAC AGCGTGCCCC TGGATGCCCA GTCTGATGAC 
AGTAAAACTT CTGTGAGGGA TCGCTTTAAT GCAAGACAGT TCATGTCATG GTTACAAGAT 
GTGGATGATA AATTCGACAA ATTAAAGACC TGTCTTTTAA TGAGGCAACA GCATGAAGCT 
GCAGCTTTAA ATGCCGTCCA GAGGTTAGAA TGGCAGCTCA AACTCCAGGA ACTTGATCCT 
GCCACCTATA AATCTATCAG CATCTATGAA ATTCAGGAGT TTTACGTTCC GCTGGTTGAT 
GTTAACGATG ACTTTGAATT GACTCCTATA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_024984609.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840377.1 Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X1, mRNA.

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CloneID	OBa11572
Clone ID Related Accession (Same CDS sequence)	XM_015469150.1 , XM_024984609.1 , XM_002697744.6 , XM_002703009.5 , XM_002697744.5
Accession Version	XM_024984609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA GAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATGC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAA ACAAAAAAGG AAGCTGGAAA TAAGAAGTCC 
ACTCCAGTTA GCATTCTCTT TGGTTACCCA CTCTCAGAGC GAAAGCAGAT GGCACTTCTT 
ATGCAGATGA CAGCACGGGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCGCAAACA 
CCACCAGCCC AAAAGAAAAC TCCCAGTTCC TCATCTCGAC AAAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCAGCTA TTCGAGGAGA TGTAAAACAA 
GTCAAAGAAC TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA CGTGGGATAT TATGATGTTG CTAAGATACT GATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCCGCTAGT 
AGTGGGCACA GAGATATTGT GAAACTGCTG CTTCGTCATG GTGGAAACCC TTTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTAGATGTG GCAGAAACAG AGGAGCTGGA GTTGTTGCTG 
AAAAGAGAGG TGCCTTTGTC TGATGATGAT GAGAGTTACA CAGATTCTGA AGAGGCTCAG 
TCTGTAAATC CTTCCAGCGT TGATGAAAAT ATTGACTCTG AGACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT AGTTCCCAGT AAAGCACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGAAGAGGAA ATAAATAAAA TGATTGATGA CAGGCATATT 
CTTAGGAAAG AACTGCGAAA AGAGAATGAA TCTGAAGTAG AAAAGAGTAG TTTATTTGCA 
AAACAGGAAA AAACCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTTTTGTATT CAAGTTCTGA AAGTTCAGAT GAAGAAATTC TTCAAAACAA AAAGTGTTCT 
GCTCCATGTT CTGTCCCTGA AACATCAAAT TCTGATATTC AAACCAAAAA GGAATATGGA 
GTTTCAAATG AACACAAACA GAAAGGCAAA GTTAAAAGAA AATTAAAGAA CCAGAACAAA 
AATAAAGAGA ACCAAGAGCT GAAGCAAGAA AAAGAGGGGA AAGAAAATAC CAGAGTAACA 
AACTTGACAG TTAATACTGC ACTAGATTGT TCAGATAAGA CCAGAGATGA GGGGAATTTT 
AGGAAATCTT TTAGCCCGAA AGATGATACT TCATTACATT TATTTCATAT TTCCAGTGGT 
AAATCTCCCA AACATTCTTG TGGACTCAGT GAAAAGCAGT CAACACCACT AAAACAAGAA 
CATTCTAAAA CCTGTTTATC ACCAGGAAGT TCTGAAATGT CATTACAGCC TGATCTTGTT 
CGGTATGATA ATATAGAATC TGAATTCTTG CCAGAAAGTT CAAGTGTAAA ATCTTGTAAG 
CATAAGGAAA AAAACAAACA TCATAAAGAT TTCCACTTAG AATTTGGTGA AAAGTCAAAT 
GCCAAAATAA AAGATGATGA TCACAGTCCA ACATTTGAAA ATTCAGATTG CACACTGAAA 
AAAATGGATA AAGAGGGTAA AACATTAAAA AAACATAAGT TAAAACATAA AGAAAGGGAA 
AAAGAAAAGC ATAAAAAAGA AATTGAAGGT GAAAAGGAAA AATACAAAAG TAGGGATGGT 
ACTAAAGAGC TGCAACGGAG TGTGGAATTT GATAGAGAGT TTTGGAAAGA GAATTTTTTT 
AAAAGTGATG AAACCGAAGA TCTATTTTTA AGTATGGAAC ATGAGTCCCT AACGTTAGAA 
AAAAAGTCAA AGTTGGAGAA AAACATAAAA GATGATAAAT CAACTAAGGA AAAGCATATC 
TCCAAAGAGA GAAACTTTAA AGAAGAACGG GAAAAGATTA AAAAGGAAAG TGAGAAATCT 
TTTAGGGAGG AAAAAATAAA AGATTTAAAA GATGACAGAG AGAACGTACT TACAGATAAA 
GAAACAGAAT TCAGCTCTTT AGGTGTAAGT GCCACTGAAG AGACTATAGG GTTACATTCA 
GTGGAAAAGG AAATAGAAAT TGAAAAGCAT ATGAAAGAAA GTAAGGAAAA ATCTGAGAAA 
CGATTTCAAA CTAAAGATAA GGACATTGAG AGGGCTGACA GAAAAAATTC TGAAAAAGAG 
AAGAAGATAA AACATGAGCA TAAGTCAGAG AAAGACAGAT TAGATCCTAG TGAATGTGTC 
GACAGAATAA AAGAAAAGGA CAAGCTATAT TCACATCACA CAGAAAAATG CCATAGAGAA 
GGTGAGAAGC TAAAAAACAT TACTACCATT AAAAAAAACG ATGACAGAGA TAAAAGTAGA 
GAAAAGATGG ATAGGAAACA TGACAAAGAA AAACCTGAGA AGGAGAGACA TCCAGTAGAG 
AGCAAAGAGA AGCACTTGAT GGAAAAAAAA AACAAGCCAT CAGATAATAG TGACTATACT 
AAATCAGAAA AAAGCAAAAA TAAAGAAAAA GATAGGGAGG TAGATAAAAA GGACAAATCT 
AGAGACAGTG TAAATATAAG TAATTCCAAA CACTTCCAGG AAGAGAGAAA GTCAAGTATA 
ACAGACAGTA ATAAAGCACA ACATGAAAAA ACTCTCTCTC TCAAAGAAAA AACAAAAGAT 
GAACCTTTGA GAACTCCTGA TGGAAAAGAA AAAGATAAAA AAGATATAGA TAGATATAAA 
GAACGAGACA AACATAAGGA TAAAATTCTG AGTAGTTCAC TCAAACTAAA ATCTGAAGCA 
GATAAGCCTA AACCGAAGTC ATCACCAGCA TCAAAAGATA CTCGGCCCAA AGAAAAGAGG 
CTTGTGAATG ACGACTTAAT GCAGACCAGC TTTGAACGGA TGCTGAGCCT TAAAGACTTG 
GAAATAGAGC AGTGGCACAG AAAACACAAG GAAAAGATTA AGCAAAAAGA AAAGGAACGG 
TTGAGAAATC GCAGTTGTTT AGAACTTAAA GTAAAAGATA AAGAAAAAAC AAAGCATGCA 
CTAGCTGAGT CCAAAAATAA AGAACTTACT AGATCAAAGA GTTCTGAGTT GACTGATACT 
TACATTAAGG AGAAACACTG TAAAGATGCT GTAAGTAATA GATCCCAATC TGTGGACACC 
AAAAATACGA TGAGTTTAGG CAAGTCATCC TTTGTTTCAG ATAATAGCTT GAACAGGTCT 
CCTAGATCAG AAAGTGAAAA GCCAGGTCTC AGCTCCAGGT CTGTCTCCTT GATTTCAGTG 
GCTAGCTCAG AAGATTCCTG CCTTACTACA ATGACAACCC CAAGGCCTCC AGTTGAGTAT 
GACTCAGACT TTATGTTAGA GGGTTCGGAT TCCCAAATGT CCTTTTCCCA GTCACCGTTT 
TTGCCAGTTG CCAAATCTCC TGCCCTTCAT GACAGGGAGC TGGATAGCCT GACTGATCTA 
TCAGAGCGGA TTAAAGCATC GTATGCTAGC AGGCTTCTGA CCTCCCATCT CCGGGCATCT 
TCTGTCGAAG ATGCTAAACT AGTTACAAAT GAAGGGAGAT CAGCTGCAGA AGCCCGAAGA 
TGTAGCATGC CCTCTGTCAT TTGTGAGCAT ACAAAGCAAT TCCAAACAAT ATCAGAAGAG 
GGCAGTCAAG ATGGCTCAGC TGTGCCAAGA GAGATTTGTC CTTCTCCCAA ATCTGAGGTA 
TCCTCCAACG TGCCTGAAAG AGAACTTTTG AGCGTGTCCA ACATACATTC CAGTTTTGCA 
GCCTCTCCAA CTAGATCTAT AAACAGCAAA TATAATTCTA CTGATACAAA TGTCATCAAG 
GGCACTGCTC CCTTGGGCAT GCTGATGGGC AGTCCTGTGC ACTTAGAGTC CTCTAATCAG 
GTTGGTGTGA TCCAAAGTAA GTCATGGGAG ATGGAGTCGT TAAACACCGG AGACTTTAGC 
TGCCCAAATT CTGCTGCACC TGATCAAGAT TCTTCTGTTC AGGGTTTTTG TAATCCTGAA 
AACAAAGTAT TGAGAGAACA GAATATTGAT TTTGTGTCCA TGCACCAGAC TGAATTGCCG 
GCAAACACTT GTGCTCAGGA TCCAGCGTCC GTCCTTCCTT CCCAGCAACC TTGCTCTTCG 
CATAGCCAAT CACTTTCAGA TGCTGAATCT ATTCCCAGAC ATACTTCTTT GTCCTATGTC 
ACCACTCAAG AACCAGGTAT TCTACAGCAG AAAAATGCAA TTCAAATGAT CAGTTCTGTT 
TTGGATACTG ATACTGAACC TACAAAAGAT ATGGAAAATA CTTTTGTCCT AACAGACGTT 
CAGAAGACAG ATGCCTTTGT CCCAGTGTAC TCTGAAAGCA CTGTTGAAGA AGCAGCACCA 
AATTTTGAGA AAGCTAGTAC TTTGCCAGTA TTGCCATCAG AAAAGGACTT TAATGGAAGT 
GACGGGTCTT CCCAGCCAAA TCCTCATTAT GCATTTAGCA AGCTAATGTT TAAGTCTTCC 
AGTGGCCATG AGGCTGAAAA AAGCACTTCT GATACTCAGG ATACTTCACA TGAAAAGGAA 
AACAAACTGG AGAGTTTGGT TTTGACTCAC TTAAATGATA AGTGTGATTC TGATTTATGT 
GAAATGAATG TAGGGGTGCC AAAAGGAAAC CTGAGTGAAC CAGATAATCC AAAACATTGT 
CCTGAAAGTG AGAAGTGTTT GCTTTCCATC GAGGATGAGG AGTCACAACA AAGTACTTTA 
TCGAGTCTGG AGAACCATCC ACAGCCATCG GCTCCACCAG AAATGCATAA ATACGGACAC 
TTAGTTAAAG TAGAACTAGA GGAAAGTGCT GAAGATGATA AACCTGAAAG CCAGATCCCT 
CAAAGGATGA CCAGAAACAA AGGAAACACA GGAGCCAGTC AAAGCAAGCA GCTTCTGGCC 
AGCTGTGCAC TGTTGCCAGA CAAAGACAGT GAGTCTGCGT CCCCTCGAGG AAGAATAAGA 
TTAACTGAGG AGGACGACCC TCAGATTCAC CACCCGCGGA AAAGGAAAGT GTCCCGGGTG 
CCTCAGCCGG TGCAAGTGAG TCCTTCTCTG CTGCAAGCAA AAGAGAAAAC CCAGCAGTCC 
CTGGCCGCCA TTGTAGACTC GCTGAAGCTC GATGAGATTC AGCCATACAG CTCAGAGCGC 
GCAAATCCCT ATTTTGAATA CTTGCACATA AGAAAAAAGA TTGAAGAAAA GCGCAAGTTG 
TTATGTAGTG TGATCCCTCA GGCACCTCAG TATTATGATG AATACGTGAC GTTTAATGGG 
TCGTATCTCC TGGACGGAAA CCCCTTAAGC AAGATCTGCA TACCCACAAT TACACCCCCA 
CCTTCACTGT CAGATCCACT TAAAGAGCTT TTTCGACAAC AGGAAGTTGT CAGGATGAAA 
CTACGTTTGC AGCACAGTAT TGAAAGGGAA AAACTCATTG TATCCAATGA ACAGGAGGTT 
CTTCGGGTTC ATTACAGAGC TGCAAGGACA CTGGCAAATC AGACACTGCC ATTCAGCGCA 
TGCACGGTTC TCCTGGATGC GGAAGTGTAC AGCGTGCCCC TGGATGCCCA GTCTGATGAC 
AGTAAAACTT CTGTGAGGGA TCGCTTTAAT GCAAGACAGT TCATGTCATG GTTACAAGAT 
GTGGATGATA AATTCGACAA ATTAAAGACC TGTCTTTTAA TGAGGCAACA GCATGAAGCT 
GCAGCTTTAA ATGCCGTCCA GAGGTTAGAA TGGCAGCTCA AACTCCAGGA ACTTGATCCT 
GCCACCTATA AATCTATCAG CATCTATGAA ATTCAGGAGT TTTACGTTCC GCTGGTTGAT 
GTTAACGATG ACTTTGAATT GACTCCTATA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840377.1
CDS	363..6515
Translation	MPKSGFTKPVQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIDRNDVGKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTCSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTPPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQIVPSKAPLPSALDEYEFKDDDEEEINKMIDDRHILRKELRKENESEVEKSSLFAKQEKTFYPKSFKSKKQKPSRVLYSSSESSDEEILQNKKCSAPCSVPETSNSDIQTKKEYGVSNEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRVTNLTVNTALDCSDKTRDEGNFRKSFSPKDDTSLHLFHISSGKSPKHSCGLSEKQSTPLKQEHSKTCLSPGSSEMSLQPDLVRYDNIESEFLPESSSVKSCKHKEKNKHHKDFHLEFGEKSNAKIKDDDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKSRDGTKELQRSVEFDREFWKENFFKSDETEDLFLSMEHESLTLEKKSKLEKNIKDDKSTKEKHISKERNFKEEREKIKKESEKSFREEKIKDLKDDRENVLTDKETEFSSLGVSATEETIGLHSVEKEIEIEKHMKESKEKSEKRFQTKDKDIERADRKNSEKEKKIKHEHKSEKDRLDPSECVDRIKEKDKLYSHHTEKCHREGEKLKNITTIKKNDDRDKSREKMDRKHDKEKPEKERHPVESKEKHLMEKKNKPSDNSDYTKSEKSKNKEKDREVDKKDKSRDSVNISNSKHFQEERKSSITDSNKAQHEKTLSLKEKTKDEPLRTPDGKEKDKKDIDRYKERDKHKDKILSSSLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRSCLELKVKDKEKTKHALAESKNKELTRSKSSELTDTYIKEKHCKDAVSNRSQSVDTKNTMSLGKSSFVSDNSLNRSPRSESEKPGLSSRSVSLISVASSEDSCLTTMTTPRPPVEYDSDFMLEGSDSQMSFSQSPFLPVAKSPALHDRELDSLTDLSERIKASYASRLLTSHLRASSVEDAKLVTNEGRSAAEARRCSMPSVICEHTKQFQTISEEGSQDGSAVPREICPSPKSEVSSNVPERELLSVSNIHSSFAASPTRSINSKYNSTDTNVIKGTAPLGMLMGSPVHLESSNQVGVIQSKSWEMESLNTGDFSCPNSAAPDQDSSVQGFCNPENKVLREQNIDFVSMHQTELPANTCAQDPASVLPSQQPCSSHSQSLSDAESIPRHTSLSYVTTQEPGILQQKNAIQMISSVLDTDTEPTKDMENTFVLTDVQKTDAFVPVYSESTVEEAAPNFEKASTLPVLPSEKDFNGSDGSSQPNPHYAFSKLMFKSSSGHEAEKSTSDTQDTSHEKENKLESLVLTHLNDKCDSDLCEMNVGVPKGNLSEPDNPKHCPESEKCLLSIEDEESQQSTLSSLENHPQPSAPPEMHKYGHLVKVELEESAEDDKPESQIPQRMTRNKGNTGASQSKQLLASCALLPDKDSESASPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYSVPLDAQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_024984609.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984609.1

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA GAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATGC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAA ACAAAAAAGG AAGCTGGAAA TAAGAAGTCC 
ACTCCAGTTA GCATTCTCTT TGGTTACCCA CTCTCAGAGC GAAAGCAGAT GGCACTTCTT 
ATGCAGATGA CAGCACGGGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCGCAAACA 
CCACCAGCCC AAAAGAAAAC TCCCAGTTCC TCATCTCGAC AAAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCAGCTA TTCGAGGAGA TGTAAAACAA 
GTCAAAGAAC TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA CGTGGGATAT TATGATGTTG CTAAGATACT GATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCCGCTAGT 
AGTGGGCACA GAGATATTGT GAAACTGCTG CTTCGTCATG GTGGAAACCC TTTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTAGATGTG GCAGAAACAG AGGAGCTGGA GTTGTTGCTG 
AAAAGAGAGG TGCCTTTGTC TGATGATGAT GAGAGTTACA CAGATTCTGA AGAGGCTCAG 
TCTGTAAATC CTTCCAGCGT TGATGAAAAT ATTGACTCTG AGACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT AGTTCCCAGT AAAGCACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGAAGAGGAA ATAAATAAAA TGATTGATGA CAGGCATATT 
CTTAGGAAAG AACTGCGAAA AGAGAATGAA TCTGAAGTAG AAAAGAGTAG TTTATTTGCA 
AAACAGGAAA AAACCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTTTTGTATT CAAGTTCTGA AAGTTCAGAT GAAGAAATTC TTCAAAACAA AAAGTGTTCT 
GCTCCATGTT CTGTCCCTGA AACATCAAAT TCTGATATTC AAACCAAAAA GGAATATGGA 
GTTTCAAATG AACACAAACA GAAAGGCAAA GTTAAAAGAA AATTAAAGAA CCAGAACAAA 
AATAAAGAGA ACCAAGAGCT GAAGCAAGAA AAAGAGGGGA AAGAAAATAC CAGAGTAACA 
AACTTGACAG TTAATACTGC ACTAGATTGT TCAGATAAGA CCAGAGATGA GGGGAATTTT 
AGGAAATCTT TTAGCCCGAA AGATGATACT TCATTACATT TATTTCATAT TTCCAGTGGT 
AAATCTCCCA AACATTCTTG TGGACTCAGT GAAAAGCAGT CAACACCACT AAAACAAGAA 
CATTCTAAAA CCTGTTTATC ACCAGGAAGT TCTGAAATGT CATTACAGCC TGATCTTGTT 
CGGTATGATA ATATAGAATC TGAATTCTTG CCAGAAAGTT CAAGTGTAAA ATCTTGTAAG 
CATAAGGAAA AAAACAAACA TCATAAAGAT TTCCACTTAG AATTTGGTGA AAAGTCAAAT 
GCCAAAATAA AAGATGATGA TCACAGTCCA ACATTTGAAA ATTCAGATTG CACACTGAAA 
AAAATGGATA AAGAGGGTAA AACATTAAAA AAACATAAGT TAAAACATAA AGAAAGGGAA 
AAAGAAAAGC ATAAAAAAGA AATTGAAGGT GAAAAGGAAA AATACAAAAG TAGGGATGGT 
ACTAAAGAGC TGCAACGGAG TGTGGAATTT GATAGAGAGT TTTGGAAAGA GAATTTTTTT 
AAAAGTGATG AAACCGAAGA TCTATTTTTA AGTATGGAAC ATGAGTCCCT AACGTTAGAA 
AAAAAGTCAA AGTTGGAGAA AAACATAAAA GATGATAAAT CAACTAAGGA AAAGCATATC 
TCCAAAGAGA GAAACTTTAA AGAAGAACGG GAAAAGATTA AAAAGGAAAG TGAGAAATCT 
TTTAGGGAGG AAAAAATAAA AGATTTAAAA GATGACAGAG AGAACGTACT TACAGATAAA 
GAAACAGAAT TCAGCTCTTT AGGTGTAAGT GCCACTGAAG AGACTATAGG GTTACATTCA 
GTGGAAAAGG AAATAGAAAT TGAAAAGCAT ATGAAAGAAA GTAAGGAAAA ATCTGAGAAA 
CGATTTCAAA CTAAAGATAA GGACATTGAG AGGGCTGACA GAAAAAATTC TGAAAAAGAG 
AAGAAGATAA AACATGAGCA TAAGTCAGAG AAAGACAGAT TAGATCCTAG TGAATGTGTC 
GACAGAATAA AAGAAAAGGA CAAGCTATAT TCACATCACA CAGAAAAATG CCATAGAGAA 
GGTGAGAAGC TAAAAAACAT TACTACCATT AAAAAAAACG ATGACAGAGA TAAAAGTAGA 
GAAAAGATGG ATAGGAAACA TGACAAAGAA AAACCTGAGA AGGAGAGACA TCCAGTAGAG 
AGCAAAGAGA AGCACTTGAT GGAAAAAAAA AACAAGCCAT CAGATAATAG TGACTATACT 
AAATCAGAAA AAAGCAAAAA TAAAGAAAAA GATAGGGAGG TAGATAAAAA GGACAAATCT 
AGAGACAGTG TAAATATAAG TAATTCCAAA CACTTCCAGG AAGAGAGAAA GTCAAGTATA 
ACAGACAGTA ATAAAGCACA ACATGAAAAA ACTCTCTCTC TCAAAGAAAA AACAAAAGAT 
GAACCTTTGA GAACTCCTGA TGGAAAAGAA AAAGATAAAA AAGATATAGA TAGATATAAA 
GAACGAGACA AACATAAGGA TAAAATTCTG AGTAGTTCAC TCAAACTAAA ATCTGAAGCA 
GATAAGCCTA AACCGAAGTC ATCACCAGCA TCAAAAGATA CTCGGCCCAA AGAAAAGAGG 
CTTGTGAATG ACGACTTAAT GCAGACCAGC TTTGAACGGA TGCTGAGCCT TAAAGACTTG 
GAAATAGAGC AGTGGCACAG AAAACACAAG GAAAAGATTA AGCAAAAAGA AAAGGAACGG 
TTGAGAAATC GCAGTTGTTT AGAACTTAAA GTAAAAGATA AAGAAAAAAC AAAGCATGCA 
CTAGCTGAGT CCAAAAATAA AGAACTTACT AGATCAAAGA GTTCTGAGTT GACTGATACT 
TACATTAAGG AGAAACACTG TAAAGATGCT GTAAGTAATA GATCCCAATC TGTGGACACC 
AAAAATACGA TGAGTTTAGG CAAGTCATCC TTTGTTTCAG ATAATAGCTT GAACAGGTCT 
CCTAGATCAG AAAGTGAAAA GCCAGGTCTC AGCTCCAGGT CTGTCTCCTT GATTTCAGTG 
GCTAGCTCAG AAGATTCCTG CCTTACTACA ATGACAACCC CAAGGCCTCC AGTTGAGTAT 
GACTCAGACT TTATGTTAGA GGGTTCGGAT TCCCAAATGT CCTTTTCCCA GTCACCGTTT 
TTGCCAGTTG CCAAATCTCC TGCCCTTCAT GACAGGGAGC TGGATAGCCT GACTGATCTA 
TCAGAGCGGA TTAAAGCATC GTATGCTAGC AGGCTTCTGA CCTCCCATCT CCGGGCATCT 
TCTGTCGAAG ATGCTAAACT AGTTACAAAT GAAGGGAGAT CAGCTGCAGA AGCCCGAAGA 
TGTAGCATGC CCTCTGTCAT TTGTGAGCAT ACAAAGCAAT TCCAAACAAT ATCAGAAGAG 
GGCAGTCAAG ATGGCTCAGC TGTGCCAAGA GAGATTTGTC CTTCTCCCAA ATCTGAGGTA 
TCCTCCAACG TGCCTGAAAG AGAACTTTTG AGCGTGTCCA ACATACATTC CAGTTTTGCA 
GCCTCTCCAA CTAGATCTAT AAACAGCAAA TATAATTCTA CTGATACAAA TGTCATCAAG 
GGCACTGCTC CCTTGGGCAT GCTGATGGGC AGTCCTGTGC ACTTAGAGTC CTCTAATCAG 
GTTGGTGTGA TCCAAAGTAA GTCATGGGAG ATGGAGTCGT TAAACACCGG AGACTTTAGC 
TGCCCAAATT CTGCTGCACC TGATCAAGAT TCTTCTGTTC AGGGTTTTTG TAATCCTGAA 
AACAAAGTAT TGAGAGAACA GAATATTGAT TTTGTGTCCA TGCACCAGAC TGAATTGCCG 
GCAAACACTT GTGCTCAGGA TCCAGCGTCC GTCCTTCCTT CCCAGCAACC TTGCTCTTCG 
CATAGCCAAT CACTTTCAGA TGCTGAATCT ATTCCCAGAC ATACTTCTTT GTCCTATGTC 
ACCACTCAAG AACCAGGTAT TCTACAGCAG AAAAATGCAA TTCAAATGAT CAGTTCTGTT 
TTGGATACTG ATACTGAACC TACAAAAGAT ATGGAAAATA CTTTTGTCCT AACAGACGTT 
CAGAAGACAG ATGCCTTTGT CCCAGTGTAC TCTGAAAGCA CTGTTGAAGA AGCAGCACCA 
AATTTTGAGA AAGCTAGTAC TTTGCCAGTA TTGCCATCAG AAAAGGACTT TAATGGAAGT 
GACGGGTCTT CCCAGCCAAA TCCTCATTAT GCATTTAGCA AGCTAATGTT TAAGTCTTCC 
AGTGGCCATG AGGCTGAAAA AAGCACTTCT GATACTCAGG ATACTTCACA TGAAAAGGAA 
AACAAACTGG AGAGTTTGGT TTTGACTCAC TTAAATGATA AGTGTGATTC TGATTTATGT 
GAAATGAATG TAGGGGTGCC AAAAGGAAAC CTGAGTGAAC CAGATAATCC AAAACATTGT 
CCTGAAAGTG AGAAGTGTTT GCTTTCCATC GAGGATGAGG AGTCACAACA AAGTACTTTA 
TCGAGTCTGG AGAACCATCC ACAGCCATCG GCTCCACCAG AAATGCATAA ATACGGACAC 
TTAGTTAAAG TAGAACTAGA GGAAAGTGCT GAAGATGATA AACCTGAAAG CCAGATCCCT 
CAAAGGATGA CCAGAAACAA AGGAAACACA GGAGCCAGTC AAAGCAAGCA GCTTCTGGCC 
AGCTGTGCAC TGTTGCCAGA CAAAGACAGT GAGTCTGCGT CCCCTCGAGG AAGAATAAGA 
TTAACTGAGG AGGACGACCC TCAGATTCAC CACCCGCGGA AAAGGAAAGT GTCCCGGGTG 
CCTCAGCCGG TGCAAGTGAG TCCTTCTCTG CTGCAAGCAA AAGAGAAAAC CCAGCAGTCC 
CTGGCCGCCA TTGTAGACTC GCTGAAGCTC GATGAGATTC AGCCATACAG CTCAGAGCGC 
GCAAATCCCT ATTTTGAATA CTTGCACATA AGAAAAAAGA TTGAAGAAAA GCGCAAGTTG 
TTATGTAGTG TGATCCCTCA GGCACCTCAG TATTATGATG AATACGTGAC GTTTAATGGG 
TCGTATCTCC TGGACGGAAA CCCCTTAAGC AAGATCTGCA TACCCACAAT TACACCCCCA 
CCTTCACTGT CAGATCCACT TAAAGAGCTT TTTCGACAAC AGGAAGTTGT CAGGATGAAA 
CTACGTTTGC AGCACAGTAT TGAAAGGGAA AAACTCATTG TATCCAATGA ACAGGAGGTT 
CTTCGGGTTC ATTACAGAGC TGCAAGGACA CTGGCAAATC AGACACTGCC ATTCAGCGCA 
TGCACGGTTC TCCTGGATGC GGAAGTGTAC AGCGTGCCCC TGGATGCCCA GTCTGATGAC 
AGTAAAACTT CTGTGAGGGA TCGCTTTAAT GCAAGACAGT TCATGTCATG GTTACAAGAT 
GTGGATGATA AATTCGACAA ATTAAAGACC TGTCTTTTAA TGAGGCAACA GCATGAAGCT 
GCAGCTTTAA ATGCCGTCCA GAGGTTAGAA TGGCAGCTCA AACTCCAGGA ACTTGATCCT 
GCCACCTATA AATCTATCAG CATCTATGAA ATTCAGGAGT TTTACGTTCC GCTGGTTGAT 
GTTAACGATG ACTTTGAATT GACTCCTATA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_002697744.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697790.1 Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X2, mRNA.

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CloneID	OBa11572
Clone ID Related Accession (Same CDS sequence)	XM_015469150.1 , XM_024984609.1 , XM_002697744.6 , XM_002703009.5 , XM_002697744.5
Accession Version	XM_002697744.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002697744.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA GAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATGC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAA ACAAAAAAGG AAGCTGGAAA TAAGAAGTCC 
ACTCCAGTTA GCATTCTCTT TGGTTACCCA CTCTCAGAGC GAAAGCAGAT GGCACTTCTT 
ATGCAGATGA CAGCACGGGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCGCAAACA 
CCACCAGCCC AAAAGAAAAC TCCCAGTTCC TCATCTCGAC AAAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCAGCTA TTCGAGGAGA TGTAAAACAA 
GTCAAAGAAC TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA CGTGGGATAT TATGATGTTG CTAAGATACT GATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCCGCTAGT 
AGTGGGCACA GAGATATTGT GAAACTGCTG CTTCGTCATG GTGGAAACCC TTTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTAGATGTG GCAGAAACAG AGGAGCTGGA GTTGTTGCTG 
AAAAGAGAGG TGCCTTTGTC TGATGATGAT GAGAGTTACA CAGATTCTGA AGAGGCTCAG 
TCTGTAAATC CTTCCAGCGT TGATGAAAAT ATTGACTCTG AGACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT AGTTCCCAGT AAAGCACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGAAGAGGAA ATAAATAAAA TGATTGATGA CAGGCATATT 
CTTAGGAAAG AACTGCGAAA AGAGAATGAA TCTGAAGTAG AAAAGAGTAG TTTATTTGCA 
AAACAGGAAA AAACCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTTTTGTATT CAAGTTCTGA AAGTTCAGAT GAAGAAATTC TTCAAAACAA AAAGTGTTCT 
GCTCCATGTT CTGTCCCTGA AACATCAAAT TCTGATATTC AAACCAAAAA GGAATATGGA 
GTTTCAAATG AACACAAACA GAAAGGCAAA GTTAAAAGAA AATTAAAGAA CCAGAACAAA 
AATAAAGAGA ACCAAGAGCT GAAGCAAGAA AAAGAGGGGA AAGAAAATAC CAGAGTAACA 
AACTTGACAG TTAATACTGC ACTAGATTGT TCAGATAAGA CCAGAGATGA GGGGAATTTT 
AGGAAATCTT TTAGCCCGAA AGATGATACT TCATTACATT TATTTCATAT TTCCAGTGGT 
AAATCTCCCA AACATTCTTG TGGACTCAGT GAAAAGCAGT CAACACCACT AAAACAAGAA 
CATTCTAAAA CCTGTTTATC ACCAGGAAGT TCTGAAATGT CATTACAGCC TGATCTTGTT 
CGGTATGATA ATATAGAATC TGAATTCTTG CCAGAAAGTT CAAGTGTAAA ATCTTGTAAG 
CATAAGGAAA AAAACAAACA TCATAAAGAT TTCCACTTAG AATTTGGTGA AAAGTCAAAT 
GCCAAAATAA AAGATGATGA TCACAGTCCA ACATTTGAAA ATTCAGATTG CACACTGAAA 
AAAATGGATA AAGAGGGTAA AACATTAAAA AAACATAAGT TAAAACATAA AGAAAGGGAA 
AAAGAAAAGC ATAAAAAAGA AATTGAAGGT GAAAAGGAAA AATACAAAAG TAGGGATGGT 
ACTAAAGAGC TGCAACGGAG TGTGGAATTT GATAGAGAGT TTTGGAAAGA GAATTTTTTT 
AAAAGTGATG AAACCGAAGA TCTATTTTTA AGTATGGAAC ATGAGTCCCT AACGTTAGAA 
AAAAAGTCAA AGTTGGAGAA AAACATAAAA GATGATAAAT CAACTAAGGA AAAGCATATC 
TCCAAAGAGA GAAACTTTAA AGAAGAACGG GAAAAGATTA AAAAGGAAAG TGAGAAATCT 
TTTAGGGAGG AAAAAATAAA AGATTTAAAA GATGACAGAG AGAACGTACT TACAGATAAA 
GAAACAGAAT TCAGCTCTTT AGGTGTAAGT GCCACTGAAG AGACTATAGG GTTACATTCA 
GTGGAAAAGG AAATAGAAAT TGAAAAGCAT ATGAAAGAAA GTAAGGAAAA ATCTGAGAAA 
CGATTTCAAA CTAAAGATAA GGACATTGAG AGGGCTGACA GAAAAAATTC TGAAAAAGAG 
AAGAAGATAA AACATGAGCA TAAGTCAGAG AAAGACAGAT TAGATCCTAG TGAATGTGTC 
GACAGAATAA AAGAAAAGGA CAAGCTATAT TCACATCACA CAGAAAAATG CCATAGAGAA 
GGTGAGAAGC TAAAAAACAT TACTACCATT AAAAAAAACG ATGACAGAGA TAAAAGTAGA 
GAAAAGATGG ATAGGAAACA TGACAAAGAA AAACCTGAGA AGGAGAGACA TCCAGTAGAG 
AGCAAAGAGA AGCACTTGAT GGAAAAAAAA AACAAGCCAT CAGATAATAG TGACTATACT 
AAATCAGAAA AAAGCAAAAA TAAAGAAAAA GATAGGGAGG TAGATAAAAA GGACAAATCT 
AGAGACAGTG TAAATATAAG TAATTCCAAA CACTTCCAGG AAGAGAGAAA GTCAAGTATA 
ACAGACAGTA ATAAAGCACA ACATGAAAAA ACTCTCTCTC TCAAAGAAAA AACAAAAGAT 
GAACCTTTGA GAACTCCTGA TGGAAAAGAA AAAGATAAAA AAGATATAGA TAGATATAAA 
GAACGAGACA AACATAAGGA TAAAATTCTG AGTAGTTCAC TCAAACTAAA ATCTGAAGCA 
GATAAGCCTA AACCGAAGTC ATCACCAGCA TCAAAAGATA CTCGGCCCAA AGAAAAGAGG 
CTTGTGAATG ACGACTTAAT GCAGACCAGC TTTGAACGGA TGCTGAGCCT TAAAGACTTG 
GAAATAGAGC AGTGGCACAG AAAACACAAG GAAAAGATTA AGCAAAAAGA AAAGGAACGG 
TTGAGAAATC GCAGTTGTTT AGAACTTAAA GTAAAAGATA AAGAAAAAAC AAAGCATGCA 
CTAGCTGAGT CCAAAAATAA AGAACTTACT AGATCAAAGA GTTCTGAGTT GACTGATACT 
TACATTAAGG AGAAACACTG TAAAGATGCT GTAAGTAATA GATCCCAATC TGTGGACACC 
AAAAATACGA TGAGTTTAGG CAAGTCATCC TTTGTTTCAG ATAATAGCTT GAACAGGTCT 
CCTAGATCAG AAAGTGAAAA GCCAGGTCTC AGCTCCAGGT CTGTCTCCTT GATTTCAGTG 
GCTAGCTCAG AAGATTCCTG CCTTACTACA ATGACAACCC CAAGGCCTCC AGTTGAGTAT 
GACTCAGACT TTATGTTAGA GGGTTCGGAT TCCCAAATGT CCTTTTCCCA GTCACCGTTT 
TTGCCAGTTG CCAAATCTCC TGCCCTTCAT GACAGGGAGC TGGATAGCCT GACTGATCTA 
TCAGAGCGGA TTAAAGCATC GTATGCTAGC AGGCTTCTGA CCTCCCATCT CCGGGCATCT 
TCTGTCGAAG ATGCTAAACT AGTTACAAAT GAAGGGAGAT CAGCTGCAGA AGCCCGAAGA 
TGTAGCATGC CCTCTGTCAT TTGTGAGCAT ACAAAGCAAT TCCAAACAAT ATCAGAAGAG 
GGCAGTCAAG ATGGCTCAGC TGTGCCAAGA GAGATTTGTC CTTCTCCCAA ATCTGAGGTA 
TCCTCCAACG TGCCTGAAAG AGAACTTTTG AGCGTGTCCA ACATACATTC CAGTTTTGCA 
GCCTCTCCAA CTAGATCTAT AAACAGCAAA TATAATTCTA CTGATACAAA TGTCATCAAG 
GGCACTGCTC CCTTGGGCAT GCTGATGGGC AGTCCTGTGC ACTTAGAGTC CTCTAATCAG 
GTTGGTGTGA TCCAAAGTAA GTCATGGGAG ATGGAGTCGT TAAACACCGG AGACTTTAGC 
TGCCCAAATT CTGCTGCACC TGATCAAGAT TCTTCTGTTC AGGGTTTTTG TAATCCTGAA 
AACAAAGTAT TGAGAGAACA GAATATTGAT TTTGTGTCCA TGCACCAGAC TGAATTGCCG 
GCAAACACTT GTGCTCAGGA TCCAGCGTCC GTCCTTCCTT CCCAGCAACC TTGCTCTTCG 
CATAGCCAAT CACTTTCAGA TGCTGAATCT ATTCCCAGAC ATACTTCTTT GTCCTATGTC 
ACCACTCAAG AACCAGGTAT TCTACAGCAG AAAAATGCAA TTCAAATGAT CAGTTCTGTT 
TTGGATACTG ATACTGAACC TACAAAAGAT ATGGAAAATA CTTTTGTCCT AACAGACGTT 
CAGAAGACAG ATGCCTTTGT CCCAGTGTAC TCTGAAAGCA CTGTTGAAGA AGCAGCACCA 
AATTTTGAGA AAGCTAGTAC TTTGCCAGTA TTGCCATCAG AAAAGGACTT TAATGGAAGT 
GACGGGTCTT CCCAGCCAAA TCCTCATTAT GCATTTAGCA AGCTAATGTT TAAGTCTTCC 
AGTGGCCATG AGGCTGAAAA AAGCACTTCT GATACTCAGG ATACTTCACA TGAAAAGGAA 
AACAAACTGG AGAGTTTGGT TTTGACTCAC TTAAATGATA AGTGTGATTC TGATTTATGT 
GAAATGAATG TAGGGGTGCC AAAAGGAAAC CTGAGTGAAC CAGATAATCC AAAACATTGT 
CCTGAAAGTG AGAAGTGTTT GCTTTCCATC GAGGATGAGG AGTCACAACA AAGTACTTTA 
TCGAGTCTGG AGAACCATCC ACAGCCATCG GCTCCACCAG AAATGCATAA ATACGGACAC 
TTAGTTAAAG TAGAACTAGA GGAAAGTGCT GAAGATGATA AACCTGAAAG CCAGATCCCT 
CAAAGGATGA CCAGAAACAA AGGAAACACA GGAGCCAGTC AAAGCAAGCA GCTTCTGGCC 
AGCTGTGCAC TGTTGCCAGA CAAAGACAGT GAGTCTGCGT CCCCTCGAGG AAGAATAAGA 
TTAACTGAGG AGGACGACCC TCAGATTCAC CACCCGCGGA AAAGGAAAGT GTCCCGGGTG 
CCTCAGCCGG TGCAAGTGAG TCCTTCTCTG CTGCAAGCAA AAGAGAAAAC CCAGCAGTCC 
CTGGCCGCCA TTGTAGACTC GCTGAAGCTC GATGAGATTC AGCCATACAG CTCAGAGCGC 
GCAAATCCCT ATTTTGAATA CTTGCACATA AGAAAAAAGA TTGAAGAAAA GCGCAAGTTG 
TTATGTAGTG TGATCCCTCA GGCACCTCAG TATTATGATG AATACGTGAC GTTTAATGGG 
TCGTATCTCC TGGACGGAAA CCCCTTAAGC AAGATCTGCA TACCCACAAT TACACCCCCA 
CCTTCACTGT CAGATCCACT TAAAGAGCTT TTTCGACAAC AGGAAGTTGT CAGGATGAAA 
CTACGTTTGC AGCACAGTAT TGAAAGGGAA AAACTCATTG TATCCAATGA ACAGGAGGTT 
CTTCGGGTTC ATTACAGAGC TGCAAGGACA CTGGCAAATC AGACACTGCC ATTCAGCGCA 
TGCACGGTTC TCCTGGATGC GGAAGTGTAC AGCGTGCCCC TGGATGCCCA GTCTGATGAC 
AGTAAAACTT CTGTGAGGGA TCGCTTTAAT GCAAGACAGT TCATGTCATG GTTACAAGAT 
GTGGATGATA AATTCGACAA ATTAAAGACC TGTCTTTTAA TGAGGCAACA GCATGAAGCT 
GCAGCTTTAA ATGCCGTCCA GAGGTTAGAA TGGCAGCTCA AACTCCAGGA ACTTGATCCT 
GCCACCTATA AATCTATCAG CATCTATGAA ATTCAGGAGT TTTACGTTCC GCTGGTTGAT 
GTTAACGATG ACTTTGAATT GACTCCTATA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697790.1
CDS	252..6404
Translation	MPKSGFTKPVQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIDRNDVGKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTCSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTPPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQIVPSKAPLPSALDEYEFKDDDEEEINKMIDDRHILRKELRKENESEVEKSSLFAKQEKTFYPKSFKSKKQKPSRVLYSSSESSDEEILQNKKCSAPCSVPETSNSDIQTKKEYGVSNEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRVTNLTVNTALDCSDKTRDEGNFRKSFSPKDDTSLHLFHISSGKSPKHSCGLSEKQSTPLKQEHSKTCLSPGSSEMSLQPDLVRYDNIESEFLPESSSVKSCKHKEKNKHHKDFHLEFGEKSNAKIKDDDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKSRDGTKELQRSVEFDREFWKENFFKSDETEDLFLSMEHESLTLEKKSKLEKNIKDDKSTKEKHISKERNFKEEREKIKKESEKSFREEKIKDLKDDRENVLTDKETEFSSLGVSATEETIGLHSVEKEIEIEKHMKESKEKSEKRFQTKDKDIERADRKNSEKEKKIKHEHKSEKDRLDPSECVDRIKEKDKLYSHHTEKCHREGEKLKNITTIKKNDDRDKSREKMDRKHDKEKPEKERHPVESKEKHLMEKKNKPSDNSDYTKSEKSKNKEKDREVDKKDKSRDSVNISNSKHFQEERKSSITDSNKAQHEKTLSLKEKTKDEPLRTPDGKEKDKKDIDRYKERDKHKDKILSSSLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRSCLELKVKDKEKTKHALAESKNKELTRSKSSELTDTYIKEKHCKDAVSNRSQSVDTKNTMSLGKSSFVSDNSLNRSPRSESEKPGLSSRSVSLISVASSEDSCLTTMTTPRPPVEYDSDFMLEGSDSQMSFSQSPFLPVAKSPALHDRELDSLTDLSERIKASYASRLLTSHLRASSVEDAKLVTNEGRSAAEARRCSMPSVICEHTKQFQTISEEGSQDGSAVPREICPSPKSEVSSNVPERELLSVSNIHSSFAASPTRSINSKYNSTDTNVIKGTAPLGMLMGSPVHLESSNQVGVIQSKSWEMESLNTGDFSCPNSAAPDQDSSVQGFCNPENKVLREQNIDFVSMHQTELPANTCAQDPASVLPSQQPCSSHSQSLSDAESIPRHTSLSYVTTQEPGILQQKNAIQMISSVLDTDTEPTKDMENTFVLTDVQKTDAFVPVYSESTVEEAAPNFEKASTLPVLPSEKDFNGSDGSSQPNPHYAFSKLMFKSSSGHEAEKSTSDTQDTSHEKENKLESLVLTHLNDKCDSDLCEMNVGVPKGNLSEPDNPKHCPESEKCLLSIEDEESQQSTLSSLENHPQPSAPPEMHKYGHLVKVELEESAEDDKPESQIPQRMTRNKGNTGASQSKQLLASCALLPDKDSESASPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYSVPLDAQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_002697744.6
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697744.6

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA GAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATGC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAA ACAAAAAAGG AAGCTGGAAA TAAGAAGTCC 
ACTCCAGTTA GCATTCTCTT TGGTTACCCA CTCTCAGAGC GAAAGCAGAT GGCACTTCTT 
ATGCAGATGA CAGCACGGGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCGCAAACA 
CCACCAGCCC AAAAGAAAAC TCCCAGTTCC TCATCTCGAC AAAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCAGCTA TTCGAGGAGA TGTAAAACAA 
GTCAAAGAAC TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA CGTGGGATAT TATGATGTTG CTAAGATACT GATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCCGCTAGT 
AGTGGGCACA GAGATATTGT GAAACTGCTG CTTCGTCATG GTGGAAACCC TTTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTAGATGTG GCAGAAACAG AGGAGCTGGA GTTGTTGCTG 
AAAAGAGAGG TGCCTTTGTC TGATGATGAT GAGAGTTACA CAGATTCTGA AGAGGCTCAG 
TCTGTAAATC CTTCCAGCGT TGATGAAAAT ATTGACTCTG AGACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT AGTTCCCAGT AAAGCACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGAAGAGGAA ATAAATAAAA TGATTGATGA CAGGCATATT 
CTTAGGAAAG AACTGCGAAA AGAGAATGAA TCTGAAGTAG AAAAGAGTAG TTTATTTGCA 
AAACAGGAAA AAACCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTTTTGTATT CAAGTTCTGA AAGTTCAGAT GAAGAAATTC TTCAAAACAA AAAGTGTTCT 
GCTCCATGTT CTGTCCCTGA AACATCAAAT TCTGATATTC AAACCAAAAA GGAATATGGA 
GTTTCAAATG AACACAAACA GAAAGGCAAA GTTAAAAGAA AATTAAAGAA CCAGAACAAA 
AATAAAGAGA ACCAAGAGCT GAAGCAAGAA AAAGAGGGGA AAGAAAATAC CAGAGTAACA 
AACTTGACAG TTAATACTGC ACTAGATTGT TCAGATAAGA CCAGAGATGA GGGGAATTTT 
AGGAAATCTT TTAGCCCGAA AGATGATACT TCATTACATT TATTTCATAT TTCCAGTGGT 
AAATCTCCCA AACATTCTTG TGGACTCAGT GAAAAGCAGT CAACACCACT AAAACAAGAA 
CATTCTAAAA CCTGTTTATC ACCAGGAAGT TCTGAAATGT CATTACAGCC TGATCTTGTT 
CGGTATGATA ATATAGAATC TGAATTCTTG CCAGAAAGTT CAAGTGTAAA ATCTTGTAAG 
CATAAGGAAA AAAACAAACA TCATAAAGAT TTCCACTTAG AATTTGGTGA AAAGTCAAAT 
GCCAAAATAA AAGATGATGA TCACAGTCCA ACATTTGAAA ATTCAGATTG CACACTGAAA 
AAAATGGATA AAGAGGGTAA AACATTAAAA AAACATAAGT TAAAACATAA AGAAAGGGAA 
AAAGAAAAGC ATAAAAAAGA AATTGAAGGT GAAAAGGAAA AATACAAAAG TAGGGATGGT 
ACTAAAGAGC TGCAACGGAG TGTGGAATTT GATAGAGAGT TTTGGAAAGA GAATTTTTTT 
AAAAGTGATG AAACCGAAGA TCTATTTTTA AGTATGGAAC ATGAGTCCCT AACGTTAGAA 
AAAAAGTCAA AGTTGGAGAA AAACATAAAA GATGATAAAT CAACTAAGGA AAAGCATATC 
TCCAAAGAGA GAAACTTTAA AGAAGAACGG GAAAAGATTA AAAAGGAAAG TGAGAAATCT 
TTTAGGGAGG AAAAAATAAA AGATTTAAAA GATGACAGAG AGAACGTACT TACAGATAAA 
GAAACAGAAT TCAGCTCTTT AGGTGTAAGT GCCACTGAAG AGACTATAGG GTTACATTCA 
GTGGAAAAGG AAATAGAAAT TGAAAAGCAT ATGAAAGAAA GTAAGGAAAA ATCTGAGAAA 
CGATTTCAAA CTAAAGATAA GGACATTGAG AGGGCTGACA GAAAAAATTC TGAAAAAGAG 
AAGAAGATAA AACATGAGCA TAAGTCAGAG AAAGACAGAT TAGATCCTAG TGAATGTGTC 
GACAGAATAA AAGAAAAGGA CAAGCTATAT TCACATCACA CAGAAAAATG CCATAGAGAA 
GGTGAGAAGC TAAAAAACAT TACTACCATT AAAAAAAACG ATGACAGAGA TAAAAGTAGA 
GAAAAGATGG ATAGGAAACA TGACAAAGAA AAACCTGAGA AGGAGAGACA TCCAGTAGAG 
AGCAAAGAGA AGCACTTGAT GGAAAAAAAA AACAAGCCAT CAGATAATAG TGACTATACT 
AAATCAGAAA AAAGCAAAAA TAAAGAAAAA GATAGGGAGG TAGATAAAAA GGACAAATCT 
AGAGACAGTG TAAATATAAG TAATTCCAAA CACTTCCAGG AAGAGAGAAA GTCAAGTATA 
ACAGACAGTA ATAAAGCACA ACATGAAAAA ACTCTCTCTC TCAAAGAAAA AACAAAAGAT 
GAACCTTTGA GAACTCCTGA TGGAAAAGAA AAAGATAAAA AAGATATAGA TAGATATAAA 
GAACGAGACA AACATAAGGA TAAAATTCTG AGTAGTTCAC TCAAACTAAA ATCTGAAGCA 
GATAAGCCTA AACCGAAGTC ATCACCAGCA TCAAAAGATA CTCGGCCCAA AGAAAAGAGG 
CTTGTGAATG ACGACTTAAT GCAGACCAGC TTTGAACGGA TGCTGAGCCT TAAAGACTTG 
GAAATAGAGC AGTGGCACAG AAAACACAAG GAAAAGATTA AGCAAAAAGA AAAGGAACGG 
TTGAGAAATC GCAGTTGTTT AGAACTTAAA GTAAAAGATA AAGAAAAAAC AAAGCATGCA 
CTAGCTGAGT CCAAAAATAA AGAACTTACT AGATCAAAGA GTTCTGAGTT GACTGATACT 
TACATTAAGG AGAAACACTG TAAAGATGCT GTAAGTAATA GATCCCAATC TGTGGACACC 
AAAAATACGA TGAGTTTAGG CAAGTCATCC TTTGTTTCAG ATAATAGCTT GAACAGGTCT 
CCTAGATCAG AAAGTGAAAA GCCAGGTCTC AGCTCCAGGT CTGTCTCCTT GATTTCAGTG 
GCTAGCTCAG AAGATTCCTG CCTTACTACA ATGACAACCC CAAGGCCTCC AGTTGAGTAT 
GACTCAGACT TTATGTTAGA GGGTTCGGAT TCCCAAATGT CCTTTTCCCA GTCACCGTTT 
TTGCCAGTTG CCAAATCTCC TGCCCTTCAT GACAGGGAGC TGGATAGCCT GACTGATCTA 
TCAGAGCGGA TTAAAGCATC GTATGCTAGC AGGCTTCTGA CCTCCCATCT CCGGGCATCT 
TCTGTCGAAG ATGCTAAACT AGTTACAAAT GAAGGGAGAT CAGCTGCAGA AGCCCGAAGA 
TGTAGCATGC CCTCTGTCAT TTGTGAGCAT ACAAAGCAAT TCCAAACAAT ATCAGAAGAG 
GGCAGTCAAG ATGGCTCAGC TGTGCCAAGA GAGATTTGTC CTTCTCCCAA ATCTGAGGTA 
TCCTCCAACG TGCCTGAAAG AGAACTTTTG AGCGTGTCCA ACATACATTC CAGTTTTGCA 
GCCTCTCCAA CTAGATCTAT AAACAGCAAA TATAATTCTA CTGATACAAA TGTCATCAAG 
GGCACTGCTC CCTTGGGCAT GCTGATGGGC AGTCCTGTGC ACTTAGAGTC CTCTAATCAG 
GTTGGTGTGA TCCAAAGTAA GTCATGGGAG ATGGAGTCGT TAAACACCGG AGACTTTAGC 
TGCCCAAATT CTGCTGCACC TGATCAAGAT TCTTCTGTTC AGGGTTTTTG TAATCCTGAA 
AACAAAGTAT TGAGAGAACA GAATATTGAT TTTGTGTCCA TGCACCAGAC TGAATTGCCG 
GCAAACACTT GTGCTCAGGA TCCAGCGTCC GTCCTTCCTT CCCAGCAACC TTGCTCTTCG 
CATAGCCAAT CACTTTCAGA TGCTGAATCT ATTCCCAGAC ATACTTCTTT GTCCTATGTC 
ACCACTCAAG AACCAGGTAT TCTACAGCAG AAAAATGCAA TTCAAATGAT CAGTTCTGTT 
TTGGATACTG ATACTGAACC TACAAAAGAT ATGGAAAATA CTTTTGTCCT AACAGACGTT 
CAGAAGACAG ATGCCTTTGT CCCAGTGTAC TCTGAAAGCA CTGTTGAAGA AGCAGCACCA 
AATTTTGAGA AAGCTAGTAC TTTGCCAGTA TTGCCATCAG AAAAGGACTT TAATGGAAGT 
GACGGGTCTT CCCAGCCAAA TCCTCATTAT GCATTTAGCA AGCTAATGTT TAAGTCTTCC 
AGTGGCCATG AGGCTGAAAA AAGCACTTCT GATACTCAGG ATACTTCACA TGAAAAGGAA 
AACAAACTGG AGAGTTTGGT TTTGACTCAC TTAAATGATA AGTGTGATTC TGATTTATGT 
GAAATGAATG TAGGGGTGCC AAAAGGAAAC CTGAGTGAAC CAGATAATCC AAAACATTGT 
CCTGAAAGTG AGAAGTGTTT GCTTTCCATC GAGGATGAGG AGTCACAACA AAGTACTTTA 
TCGAGTCTGG AGAACCATCC ACAGCCATCG GCTCCACCAG AAATGCATAA ATACGGACAC 
TTAGTTAAAG TAGAACTAGA GGAAAGTGCT GAAGATGATA AACCTGAAAG CCAGATCCCT 
CAAAGGATGA CCAGAAACAA AGGAAACACA GGAGCCAGTC AAAGCAAGCA GCTTCTGGCC 
AGCTGTGCAC TGTTGCCAGA CAAAGACAGT GAGTCTGCGT CCCCTCGAGG AAGAATAAGA 
TTAACTGAGG AGGACGACCC TCAGATTCAC CACCCGCGGA AAAGGAAAGT GTCCCGGGTG 
CCTCAGCCGG TGCAAGTGAG TCCTTCTCTG CTGCAAGCAA AAGAGAAAAC CCAGCAGTCC 
CTGGCCGCCA TTGTAGACTC GCTGAAGCTC GATGAGATTC AGCCATACAG CTCAGAGCGC 
GCAAATCCCT ATTTTGAATA CTTGCACATA AGAAAAAAGA TTGAAGAAAA GCGCAAGTTG 
TTATGTAGTG TGATCCCTCA GGCACCTCAG TATTATGATG AATACGTGAC GTTTAATGGG 
TCGTATCTCC TGGACGGAAA CCCCTTAAGC AAGATCTGCA TACCCACAAT TACACCCCCA 
CCTTCACTGT CAGATCCACT TAAAGAGCTT TTTCGACAAC AGGAAGTTGT CAGGATGAAA 
CTACGTTTGC AGCACAGTAT TGAAAGGGAA AAACTCATTG TATCCAATGA ACAGGAGGTT 
CTTCGGGTTC ATTACAGAGC TGCAAGGACA CTGGCAAATC AGACACTGCC ATTCAGCGCA 
TGCACGGTTC TCCTGGATGC GGAAGTGTAC AGCGTGCCCC TGGATGCCCA GTCTGATGAC 
AGTAAAACTT CTGTGAGGGA TCGCTTTAAT GCAAGACAGT TCATGTCATG GTTACAAGAT 
GTGGATGATA AATTCGACAA ATTAAAGACC TGTCTTTTAA TGAGGCAACA GCATGAAGCT 
GCAGCTTTAA ATGCCGTCCA GAGGTTAGAA TGGCAGCTCA AACTCCAGGA ACTTGATCCT 
GCCACCTATA AATCTATCAG CATCTATGAA ATTCAGGAGT TTTACGTTCC GCTGGTTGAT 
GTTAACGATG ACTTTGAATT GACTCCTATA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_002703009.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002703055.1 Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X5, mRNA.

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CloneID	OBa11572
Clone ID Related Accession (Same CDS sequence)	XM_015469150.1 , XM_024984609.1 , XM_002697744.6 , XM_002703009.5 , XM_002697744.5
Accession Version	XM_002703009.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002703009.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA GAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATGC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAA ACAAAAAAGG AAGCTGGAAA TAAGAAGTCC 
ACTCCAGTTA GCATTCTCTT TGGTTACCCA CTCTCAGAGC GAAAGCAGAT GGCACTTCTT 
ATGCAGATGA CAGCACGGGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCGCAAACA 
CCACCAGCCC AAAAGAAAAC TCCCAGTTCC TCATCTCGAC AAAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCAGCTA TTCGAGGAGA TGTAAAACAA 
GTCAAAGAAC TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA CGTGGGATAT TATGATGTTG CTAAGATACT GATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCCGCTAGT 
AGTGGGCACA GAGATATTGT GAAACTGCTG CTTCGTCATG GTGGAAACCC TTTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTAGATGTG GCAGAAACAG AGGAGCTGGA GTTGTTGCTG 
AAAAGAGAGG TGCCTTTGTC TGATGATGAT GAGAGTTACA CAGATTCTGA AGAGGCTCAG 
TCTGTAAATC CTTCCAGCGT TGATGAAAAT ATTGACTCTG AGACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT AGTTCCCAGT AAAGCACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGAAGAGGAA ATAAATAAAA TGATTGATGA CAGGCATATT 
CTTAGGAAAG AACTGCGAAA AGAGAATGAA TCTGAAGTAG AAAAGAGTAG TTTATTTGCA 
AAACAGGAAA AAACCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTTTTGTATT CAAGTTCTGA AAGTTCAGAT GAAGAAATTC TTCAAAACAA AAAGTGTTCT 
GCTCCATGTT CTGTCCCTGA AACATCAAAT TCTGATATTC AAACCAAAAA GGAATATGGA 
GTTTCAAATG AACACAAACA GAAAGGCAAA GTTAAAAGAA AATTAAAGAA CCAGAACAAA 
AATAAAGAGA ACCAAGAGCT GAAGCAAGAA AAAGAGGGGA AAGAAAATAC CAGAGTAACA 
AACTTGACAG TTAATACTGC ACTAGATTGT TCAGATAAGA CCAGAGATGA GGGGAATTTT 
AGGAAATCTT TTAGCCCGAA AGATGATACT TCATTACATT TATTTCATAT TTCCAGTGGT 
AAATCTCCCA AACATTCTTG TGGACTCAGT GAAAAGCAGT CAACACCACT AAAACAAGAA 
CATTCTAAAA CCTGTTTATC ACCAGGAAGT TCTGAAATGT CATTACAGCC TGATCTTGTT 
CGGTATGATA ATATAGAATC TGAATTCTTG CCAGAAAGTT CAAGTGTAAA ATCTTGTAAG 
CATAAGGAAA AAAACAAACA TCATAAAGAT TTCCACTTAG AATTTGGTGA AAAGTCAAAT 
GCCAAAATAA AAGATGATGA TCACAGTCCA ACATTTGAAA ATTCAGATTG CACACTGAAA 
AAAATGGATA AAGAGGGTAA AACATTAAAA AAACATAAGT TAAAACATAA AGAAAGGGAA 
AAAGAAAAGC ATAAAAAAGA AATTGAAGGT GAAAAGGAAA AATACAAAAG TAGGGATGGT 
ACTAAAGAGC TGCAACGGAG TGTGGAATTT GATAGAGAGT TTTGGAAAGA GAATTTTTTT 
AAAAGTGATG AAACCGAAGA TCTATTTTTA AGTATGGAAC ATGAGTCCCT AACGTTAGAA 
AAAAAGTCAA AGTTGGAGAA AAACATAAAA GATGATAAAT CAACTAAGGA AAAGCATATC 
TCCAAAGAGA GAAACTTTAA AGAAGAACGG GAAAAGATTA AAAAGGAAAG TGAGAAATCT 
TTTAGGGAGG AAAAAATAAA AGATTTAAAA GATGACAGAG AGAACGTACT TACAGATAAA 
GAAACAGAAT TCAGCTCTTT AGGTGTAAGT GCCACTGAAG AGACTATAGG GTTACATTCA 
GTGGAAAAGG AAATAGAAAT TGAAAAGCAT ATGAAAGAAA GTAAGGAAAA ATCTGAGAAA 
CGATTTCAAA CTAAAGATAA GGACATTGAG AGGGCTGACA GAAAAAATTC TGAAAAAGAG 
AAGAAGATAA AACATGAGCA TAAGTCAGAG AAAGACAGAT TAGATCCTAG TGAATGTGTC 
GACAGAATAA AAGAAAAGGA CAAGCTATAT TCACATCACA CAGAAAAATG CCATAGAGAA 
GGTGAGAAGC TAAAAAACAT TACTACCATT AAAAAAAACG ATGACAGAGA TAAAAGTAGA 
GAAAAGATGG ATAGGAAACA TGACAAAGAA AAACCTGAGA AGGAGAGACA TCCAGTAGAG 
AGCAAAGAGA AGCACTTGAT GGAAAAAAAA AACAAGCCAT CAGATAATAG TGACTATACT 
AAATCAGAAA AAAGCAAAAA TAAAGAAAAA GATAGGGAGG TAGATAAAAA GGACAAATCT 
AGAGACAGTG TAAATATAAG TAATTCCAAA CACTTCCAGG AAGAGAGAAA GTCAAGTATA 
ACAGACAGTA ATAAAGCACA ACATGAAAAA ACTCTCTCTC TCAAAGAAAA AACAAAAGAT 
GAACCTTTGA GAACTCCTGA TGGAAAAGAA AAAGATAAAA AAGATATAGA TAGATATAAA 
GAACGAGACA AACATAAGGA TAAAATTCTG AGTAGTTCAC TCAAACTAAA ATCTGAAGCA 
GATAAGCCTA AACCGAAGTC ATCACCAGCA TCAAAAGATA CTCGGCCCAA AGAAAAGAGG 
CTTGTGAATG ACGACTTAAT GCAGACCAGC TTTGAACGGA TGCTGAGCCT TAAAGACTTG 
GAAATAGAGC AGTGGCACAG AAAACACAAG GAAAAGATTA AGCAAAAAGA AAAGGAACGG 
TTGAGAAATC GCAGTTGTTT AGAACTTAAA GTAAAAGATA AAGAAAAAAC AAAGCATGCA 
CTAGCTGAGT CCAAAAATAA AGAACTTACT AGATCAAAGA GTTCTGAGTT GACTGATACT 
TACATTAAGG AGAAACACTG TAAAGATGCT GTAAGTAATA GATCCCAATC TGTGGACACC 
AAAAATACGA TGAGTTTAGG CAAGTCATCC TTTGTTTCAG ATAATAGCTT GAACAGGTCT 
CCTAGATCAG AAAGTGAAAA GCCAGGTCTC AGCTCCAGGT CTGTCTCCTT GATTTCAGTG 
GCTAGCTCAG AAGATTCCTG CCTTACTACA ATGACAACCC CAAGGCCTCC AGTTGAGTAT 
GACTCAGACT TTATGTTAGA GGGTTCGGAT TCCCAAATGT CCTTTTCCCA GTCACCGTTT 
TTGCCAGTTG CCAAATCTCC TGCCCTTCAT GACAGGGAGC TGGATAGCCT GACTGATCTA 
TCAGAGCGGA TTAAAGCATC GTATGCTAGC AGGCTTCTGA CCTCCCATCT CCGGGCATCT 
TCTGTCGAAG ATGCTAAACT AGTTACAAAT GAAGGGAGAT CAGCTGCAGA AGCCCGAAGA 
TGTAGCATGC CCTCTGTCAT TTGTGAGCAT ACAAAGCAAT TCCAAACAAT ATCAGAAGAG 
GGCAGTCAAG ATGGCTCAGC TGTGCCAAGA GAGATTTGTC CTTCTCCCAA ATCTGAGGTA 
TCCTCCAACG TGCCTGAAAG AGAACTTTTG AGCGTGTCCA ACATACATTC CAGTTTTGCA 
GCCTCTCCAA CTAGATCTAT AAACAGCAAA TATAATTCTA CTGATACAAA TGTCATCAAG 
GGCACTGCTC CCTTGGGCAT GCTGATGGGC AGTCCTGTGC ACTTAGAGTC CTCTAATCAG 
GTTGGTGTGA TCCAAAGTAA GTCATGGGAG ATGGAGTCGT TAAACACCGG AGACTTTAGC 
TGCCCAAATT CTGCTGCACC TGATCAAGAT TCTTCTGTTC AGGGTTTTTG TAATCCTGAA 
AACAAAGTAT TGAGAGAACA GAATATTGAT TTTGTGTCCA TGCACCAGAC TGAATTGCCG 
GCAAACACTT GTGCTCAGGA TCCAGCGTCC GTCCTTCCTT CCCAGCAACC TTGCTCTTCG 
CATAGCCAAT CACTTTCAGA TGCTGAATCT ATTCCCAGAC ATACTTCTTT GTCCTATGTC 
ACCACTCAAG AACCAGGTAT TCTACAGCAG AAAAATGCAA TTCAAATGAT CAGTTCTGTT 
TTGGATACTG ATACTGAACC TACAAAAGAT ATGGAAAATA CTTTTGTCCT AACAGACGTT 
CAGAAGACAG ATGCCTTTGT CCCAGTGTAC TCTGAAAGCA CTGTTGAAGA AGCAGCACCA 
AATTTTGAGA AAGCTAGTAC TTTGCCAGTA TTGCCATCAG AAAAGGACTT TAATGGAAGT 
GACGGGTCTT CCCAGCCAAA TCCTCATTAT GCATTTAGCA AGCTAATGTT TAAGTCTTCC 
AGTGGCCATG AGGCTGAAAA AAGCACTTCT GATACTCAGG ATACTTCACA TGAAAAGGAA 
AACAAACTGG AGAGTTTGGT TTTGACTCAC TTAAATGATA AGTGTGATTC TGATTTATGT 
GAAATGAATG TAGGGGTGCC AAAAGGAAAC CTGAGTGAAC CAGATAATCC AAAACATTGT 
CCTGAAAGTG AGAAGTGTTT GCTTTCCATC GAGGATGAGG AGTCACAACA AAGTACTTTA 
TCGAGTCTGG AGAACCATCC ACAGCCATCG GCTCCACCAG AAATGCATAA ATACGGACAC 
TTAGTTAAAG TAGAACTAGA GGAAAGTGCT GAAGATGATA AACCTGAAAG CCAGATCCCT 
CAAAGGATGA CCAGAAACAA AGGAAACACA GGAGCCAGTC AAAGCAAGCA GCTTCTGGCC 
AGCTGTGCAC TGTTGCCAGA CAAAGACAGT GAGTCTGCGT CCCCTCGAGG AAGAATAAGA 
TTAACTGAGG AGGACGACCC TCAGATTCAC CACCCGCGGA AAAGGAAAGT GTCCCGGGTG 
CCTCAGCCGG TGCAAGTGAG TCCTTCTCTG CTGCAAGCAA AAGAGAAAAC CCAGCAGTCC 
CTGGCCGCCA TTGTAGACTC GCTGAAGCTC GATGAGATTC AGCCATACAG CTCAGAGCGC 
GCAAATCCCT ATTTTGAATA CTTGCACATA AGAAAAAAGA TTGAAGAAAA GCGCAAGTTG 
TTATGTAGTG TGATCCCTCA GGCACCTCAG TATTATGATG AATACGTGAC GTTTAATGGG 
TCGTATCTCC TGGACGGAAA CCCCTTAAGC AAGATCTGCA TACCCACAAT TACACCCCCA 
CCTTCACTGT CAGATCCACT TAAAGAGCTT TTTCGACAAC AGGAAGTTGT CAGGATGAAA 
CTACGTTTGC AGCACAGTAT TGAAAGGGAA AAACTCATTG TATCCAATGA ACAGGAGGTT 
CTTCGGGTTC ATTACAGAGC TGCAAGGACA CTGGCAAATC AGACACTGCC ATTCAGCGCA 
TGCACGGTTC TCCTGGATGC GGAAGTGTAC AGCGTGCCCC TGGATGCCCA GTCTGATGAC 
AGTAAAACTT CTGTGAGGGA TCGCTTTAAT GCAAGACAGT TCATGTCATG GTTACAAGAT 
GTGGATGATA AATTCGACAA ATTAAAGACC TGTCTTTTAA TGAGGCAACA GCATGAAGCT 
GCAGCTTTAA ATGCCGTCCA GAGGTTAGAA TGGCAGCTCA AACTCCAGGA ACTTGATCCT 
GCCACCTATA AATCTATCAG CATCTATGAA ATTCAGGAGT TTTACGTTCC GCTGGTTGAT 
GTTAACGATG ACTTTGAATT GACTCCTATA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002703055.1
CDS	220..6372
Translation	MPKSGFTKPVQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIDRNDVGKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTCSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTPPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQIVPSKAPLPSALDEYEFKDDDEEEINKMIDDRHILRKELRKENESEVEKSSLFAKQEKTFYPKSFKSKKQKPSRVLYSSSESSDEEILQNKKCSAPCSVPETSNSDIQTKKEYGVSNEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRVTNLTVNTALDCSDKTRDEGNFRKSFSPKDDTSLHLFHISSGKSPKHSCGLSEKQSTPLKQEHSKTCLSPGSSEMSLQPDLVRYDNIESEFLPESSSVKSCKHKEKNKHHKDFHLEFGEKSNAKIKDDDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKSRDGTKELQRSVEFDREFWKENFFKSDETEDLFLSMEHESLTLEKKSKLEKNIKDDKSTKEKHISKERNFKEEREKIKKESEKSFREEKIKDLKDDRENVLTDKETEFSSLGVSATEETIGLHSVEKEIEIEKHMKESKEKSEKRFQTKDKDIERADRKNSEKEKKIKHEHKSEKDRLDPSECVDRIKEKDKLYSHHTEKCHREGEKLKNITTIKKNDDRDKSREKMDRKHDKEKPEKERHPVESKEKHLMEKKNKPSDNSDYTKSEKSKNKEKDREVDKKDKSRDSVNISNSKHFQEERKSSITDSNKAQHEKTLSLKEKTKDEPLRTPDGKEKDKKDIDRYKERDKHKDKILSSSLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRSCLELKVKDKEKTKHALAESKNKELTRSKSSELTDTYIKEKHCKDAVSNRSQSVDTKNTMSLGKSSFVSDNSLNRSPRSESEKPGLSSRSVSLISVASSEDSCLTTMTTPRPPVEYDSDFMLEGSDSQMSFSQSPFLPVAKSPALHDRELDSLTDLSERIKASYASRLLTSHLRASSVEDAKLVTNEGRSAAEARRCSMPSVICEHTKQFQTISEEGSQDGSAVPREICPSPKSEVSSNVPERELLSVSNIHSSFAASPTRSINSKYNSTDTNVIKGTAPLGMLMGSPVHLESSNQVGVIQSKSWEMESLNTGDFSCPNSAAPDQDSSVQGFCNPENKVLREQNIDFVSMHQTELPANTCAQDPASVLPSQQPCSSHSQSLSDAESIPRHTSLSYVTTQEPGILQQKNAIQMISSVLDTDTEPTKDMENTFVLTDVQKTDAFVPVYSESTVEEAAPNFEKASTLPVLPSEKDFNGSDGSSQPNPHYAFSKLMFKSSSGHEAEKSTSDTQDTSHEKENKLESLVLTHLNDKCDSDLCEMNVGVPKGNLSEPDNPKHCPESEKCLLSIEDEESQQSTLSSLENHPQPSAPPEMHKYGHLVKVELEESAEDDKPESQIPQRMTRNKGNTGASQSKQLLASCALLPDKDSESASPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYSVPLDAQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_002703009.5
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002703009.5

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA GAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATGC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAA ACAAAAAAGG AAGCTGGAAA TAAGAAGTCC 
ACTCCAGTTA GCATTCTCTT TGGTTACCCA CTCTCAGAGC GAAAGCAGAT GGCACTTCTT 
ATGCAGATGA CAGCACGGGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCGCAAACA 
CCACCAGCCC AAAAGAAAAC TCCCAGTTCC TCATCTCGAC AAAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCAGCTA TTCGAGGAGA TGTAAAACAA 
GTCAAAGAAC TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA CGTGGGATAT TATGATGTTG CTAAGATACT GATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCCGCTAGT 
AGTGGGCACA GAGATATTGT GAAACTGCTG CTTCGTCATG GTGGAAACCC TTTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTAGATGTG GCAGAAACAG AGGAGCTGGA GTTGTTGCTG 
AAAAGAGAGG TGCCTTTGTC TGATGATGAT GAGAGTTACA CAGATTCTGA AGAGGCTCAG 
TCTGTAAATC CTTCCAGCGT TGATGAAAAT ATTGACTCTG AGACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT AGTTCCCAGT AAAGCACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGAAGAGGAA ATAAATAAAA TGATTGATGA CAGGCATATT 
CTTAGGAAAG AACTGCGAAA AGAGAATGAA TCTGAAGTAG AAAAGAGTAG TTTATTTGCA 
AAACAGGAAA AAACCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTTTTGTATT CAAGTTCTGA AAGTTCAGAT GAAGAAATTC TTCAAAACAA AAAGTGTTCT 
GCTCCATGTT CTGTCCCTGA AACATCAAAT TCTGATATTC AAACCAAAAA GGAATATGGA 
GTTTCAAATG AACACAAACA GAAAGGCAAA GTTAAAAGAA AATTAAAGAA CCAGAACAAA 
AATAAAGAGA ACCAAGAGCT GAAGCAAGAA AAAGAGGGGA AAGAAAATAC CAGAGTAACA 
AACTTGACAG TTAATACTGC ACTAGATTGT TCAGATAAGA CCAGAGATGA GGGGAATTTT 
AGGAAATCTT TTAGCCCGAA AGATGATACT TCATTACATT TATTTCATAT TTCCAGTGGT 
AAATCTCCCA AACATTCTTG TGGACTCAGT GAAAAGCAGT CAACACCACT AAAACAAGAA 
CATTCTAAAA CCTGTTTATC ACCAGGAAGT TCTGAAATGT CATTACAGCC TGATCTTGTT 
CGGTATGATA ATATAGAATC TGAATTCTTG CCAGAAAGTT CAAGTGTAAA ATCTTGTAAG 
CATAAGGAAA AAAACAAACA TCATAAAGAT TTCCACTTAG AATTTGGTGA AAAGTCAAAT 
GCCAAAATAA AAGATGATGA TCACAGTCCA ACATTTGAAA ATTCAGATTG CACACTGAAA 
AAAATGGATA AAGAGGGTAA AACATTAAAA AAACATAAGT TAAAACATAA AGAAAGGGAA 
AAAGAAAAGC ATAAAAAAGA AATTGAAGGT GAAAAGGAAA AATACAAAAG TAGGGATGGT 
ACTAAAGAGC TGCAACGGAG TGTGGAATTT GATAGAGAGT TTTGGAAAGA GAATTTTTTT 
AAAAGTGATG AAACCGAAGA TCTATTTTTA AGTATGGAAC ATGAGTCCCT AACGTTAGAA 
AAAAAGTCAA AGTTGGAGAA AAACATAAAA GATGATAAAT CAACTAAGGA AAAGCATATC 
TCCAAAGAGA GAAACTTTAA AGAAGAACGG GAAAAGATTA AAAAGGAAAG TGAGAAATCT 
TTTAGGGAGG AAAAAATAAA AGATTTAAAA GATGACAGAG AGAACGTACT TACAGATAAA 
GAAACAGAAT TCAGCTCTTT AGGTGTAAGT GCCACTGAAG AGACTATAGG GTTACATTCA 
GTGGAAAAGG AAATAGAAAT TGAAAAGCAT ATGAAAGAAA GTAAGGAAAA ATCTGAGAAA 
CGATTTCAAA CTAAAGATAA GGACATTGAG AGGGCTGACA GAAAAAATTC TGAAAAAGAG 
AAGAAGATAA AACATGAGCA TAAGTCAGAG AAAGACAGAT TAGATCCTAG TGAATGTGTC 
GACAGAATAA AAGAAAAGGA CAAGCTATAT TCACATCACA CAGAAAAATG CCATAGAGAA 
GGTGAGAAGC TAAAAAACAT TACTACCATT AAAAAAAACG ATGACAGAGA TAAAAGTAGA 
GAAAAGATGG ATAGGAAACA TGACAAAGAA AAACCTGAGA AGGAGAGACA TCCAGTAGAG 
AGCAAAGAGA AGCACTTGAT GGAAAAAAAA AACAAGCCAT CAGATAATAG TGACTATACT 
AAATCAGAAA AAAGCAAAAA TAAAGAAAAA GATAGGGAGG TAGATAAAAA GGACAAATCT 
AGAGACAGTG TAAATATAAG TAATTCCAAA CACTTCCAGG AAGAGAGAAA GTCAAGTATA 
ACAGACAGTA ATAAAGCACA ACATGAAAAA ACTCTCTCTC TCAAAGAAAA AACAAAAGAT 
GAACCTTTGA GAACTCCTGA TGGAAAAGAA AAAGATAAAA AAGATATAGA TAGATATAAA 
GAACGAGACA AACATAAGGA TAAAATTCTG AGTAGTTCAC TCAAACTAAA ATCTGAAGCA 
GATAAGCCTA AACCGAAGTC ATCACCAGCA TCAAAAGATA CTCGGCCCAA AGAAAAGAGG 
CTTGTGAATG ACGACTTAAT GCAGACCAGC TTTGAACGGA TGCTGAGCCT TAAAGACTTG 
GAAATAGAGC AGTGGCACAG AAAACACAAG GAAAAGATTA AGCAAAAAGA AAAGGAACGG 
TTGAGAAATC GCAGTTGTTT AGAACTTAAA GTAAAAGATA AAGAAAAAAC AAAGCATGCA 
CTAGCTGAGT CCAAAAATAA AGAACTTACT AGATCAAAGA GTTCTGAGTT GACTGATACT 
TACATTAAGG AGAAACACTG TAAAGATGCT GTAAGTAATA GATCCCAATC TGTGGACACC 
AAAAATACGA TGAGTTTAGG CAAGTCATCC TTTGTTTCAG ATAATAGCTT GAACAGGTCT 
CCTAGATCAG AAAGTGAAAA GCCAGGTCTC AGCTCCAGGT CTGTCTCCTT GATTTCAGTG 
GCTAGCTCAG AAGATTCCTG CCTTACTACA ATGACAACCC CAAGGCCTCC AGTTGAGTAT 
GACTCAGACT TTATGTTAGA GGGTTCGGAT TCCCAAATGT CCTTTTCCCA GTCACCGTTT 
TTGCCAGTTG CCAAATCTCC TGCCCTTCAT GACAGGGAGC TGGATAGCCT GACTGATCTA 
TCAGAGCGGA TTAAAGCATC GTATGCTAGC AGGCTTCTGA CCTCCCATCT CCGGGCATCT 
TCTGTCGAAG ATGCTAAACT AGTTACAAAT GAAGGGAGAT CAGCTGCAGA AGCCCGAAGA 
TGTAGCATGC CCTCTGTCAT TTGTGAGCAT ACAAAGCAAT TCCAAACAAT ATCAGAAGAG 
GGCAGTCAAG ATGGCTCAGC TGTGCCAAGA GAGATTTGTC CTTCTCCCAA ATCTGAGGTA 
TCCTCCAACG TGCCTGAAAG AGAACTTTTG AGCGTGTCCA ACATACATTC CAGTTTTGCA 
GCCTCTCCAA CTAGATCTAT AAACAGCAAA TATAATTCTA CTGATACAAA TGTCATCAAG 
GGCACTGCTC CCTTGGGCAT GCTGATGGGC AGTCCTGTGC ACTTAGAGTC CTCTAATCAG 
GTTGGTGTGA TCCAAAGTAA GTCATGGGAG ATGGAGTCGT TAAACACCGG AGACTTTAGC 
TGCCCAAATT CTGCTGCACC TGATCAAGAT TCTTCTGTTC AGGGTTTTTG TAATCCTGAA 
AACAAAGTAT TGAGAGAACA GAATATTGAT TTTGTGTCCA TGCACCAGAC TGAATTGCCG 
GCAAACACTT GTGCTCAGGA TCCAGCGTCC GTCCTTCCTT CCCAGCAACC TTGCTCTTCG 
CATAGCCAAT CACTTTCAGA TGCTGAATCT ATTCCCAGAC ATACTTCTTT GTCCTATGTC 
ACCACTCAAG AACCAGGTAT TCTACAGCAG AAAAATGCAA TTCAAATGAT CAGTTCTGTT 
TTGGATACTG ATACTGAACC TACAAAAGAT ATGGAAAATA CTTTTGTCCT AACAGACGTT 
CAGAAGACAG ATGCCTTTGT CCCAGTGTAC TCTGAAAGCA CTGTTGAAGA AGCAGCACCA 
AATTTTGAGA AAGCTAGTAC TTTGCCAGTA TTGCCATCAG AAAAGGACTT TAATGGAAGT 
GACGGGTCTT CCCAGCCAAA TCCTCATTAT GCATTTAGCA AGCTAATGTT TAAGTCTTCC 
AGTGGCCATG AGGCTGAAAA AAGCACTTCT GATACTCAGG ATACTTCACA TGAAAAGGAA 
AACAAACTGG AGAGTTTGGT TTTGACTCAC TTAAATGATA AGTGTGATTC TGATTTATGT 
GAAATGAATG TAGGGGTGCC AAAAGGAAAC CTGAGTGAAC CAGATAATCC AAAACATTGT 
CCTGAAAGTG AGAAGTGTTT GCTTTCCATC GAGGATGAGG AGTCACAACA AAGTACTTTA 
TCGAGTCTGG AGAACCATCC ACAGCCATCG GCTCCACCAG AAATGCATAA ATACGGACAC 
TTAGTTAAAG TAGAACTAGA GGAAAGTGCT GAAGATGATA AACCTGAAAG CCAGATCCCT 
CAAAGGATGA CCAGAAACAA AGGAAACACA GGAGCCAGTC AAAGCAAGCA GCTTCTGGCC 
AGCTGTGCAC TGTTGCCAGA CAAAGACAGT GAGTCTGCGT CCCCTCGAGG AAGAATAAGA 
TTAACTGAGG AGGACGACCC TCAGATTCAC CACCCGCGGA AAAGGAAAGT GTCCCGGGTG 
CCTCAGCCGG TGCAAGTGAG TCCTTCTCTG CTGCAAGCAA AAGAGAAAAC CCAGCAGTCC 
CTGGCCGCCA TTGTAGACTC GCTGAAGCTC GATGAGATTC AGCCATACAG CTCAGAGCGC 
GCAAATCCCT ATTTTGAATA CTTGCACATA AGAAAAAAGA TTGAAGAAAA GCGCAAGTTG 
TTATGTAGTG TGATCCCTCA GGCACCTCAG TATTATGATG AATACGTGAC GTTTAATGGG 
TCGTATCTCC TGGACGGAAA CCCCTTAAGC AAGATCTGCA TACCCACAAT TACACCCCCA 
CCTTCACTGT CAGATCCACT TAAAGAGCTT TTTCGACAAC AGGAAGTTGT CAGGATGAAA 
CTACGTTTGC AGCACAGTAT TGAAAGGGAA AAACTCATTG TATCCAATGA ACAGGAGGTT 
CTTCGGGTTC ATTACAGAGC TGCAAGGACA CTGGCAAATC AGACACTGCC ATTCAGCGCA 
TGCACGGTTC TCCTGGATGC GGAAGTGTAC AGCGTGCCCC TGGATGCCCA GTCTGATGAC 
AGTAAAACTT CTGTGAGGGA TCGCTTTAAT GCAAGACAGT TCATGTCATG GTTACAAGAT 
GTGGATGATA AATTCGACAA ATTAAAGACC TGTCTTTTAA TGAGGCAACA GCATGAAGCT 
GCAGCTTTAA ATGCCGTCCA GAGGTTAGAA TGGCAGCTCA AACTCCAGGA ACTTGATCCT 
GCCACCTATA AATCTATCAG CATCTATGAA ATTCAGGAGT TTTACGTTCC GCTGGTTGAT 
GTTAACGATG ACTTTGAATT GACTCCTATA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_002697744.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697790.1 Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X1, mRNA.

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CloneID	OBa11572
Clone ID Related Accession (Same CDS sequence)	XM_015469150.1 , XM_024984609.1 , XM_002697744.6 , XM_002703009.5 , XM_002697744.5
Accession Version	XM_002697744.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002697744.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA GAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATGC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAA ACAAAAAAGG AAGCTGGAAA TAAGAAGTCC 
ACTCCAGTTA GCATTCTCTT TGGTTACCCA CTCTCAGAGC GAAAGCAGAT GGCACTTCTT 
ATGCAGATGA CAGCACGGGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCGCAAACA 
CCACCAGCCC AAAAGAAAAC TCCCAGTTCC TCATCTCGAC AAAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCAGCTA TTCGAGGAGA TGTAAAACAA 
GTCAAAGAAC TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA CGTGGGATAT TATGATGTTG CTAAGATACT GATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCCGCTAGT 
AGTGGGCACA GAGATATTGT GAAACTGCTG CTTCGTCATG GTGGAAACCC TTTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTAGATGTG GCAGAAACAG AGGAGCTGGA GTTGTTGCTG 
AAAAGAGAGG TGCCTTTGTC TGATGATGAT GAGAGTTACA CAGATTCTGA AGAGGCTCAG 
TCTGTAAATC CTTCCAGCGT TGATGAAAAT ATTGACTCTG AGACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT AGTTCCCAGT AAAGCACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGAAGAGGAA ATAAATAAAA TGATTGATGA CAGGCATATT 
CTTAGGAAAG AACTGCGAAA AGAGAATGAA TCTGAAGTAG AAAAGAGTAG TTTATTTGCA 
AAACAGGAAA AAACCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTTTTGTATT CAAGTTCTGA AAGTTCAGAT GAAGAAATTC TTCAAAACAA AAAGTGTTCT 
GCTCCATGTT CTGTCCCTGA AACATCAAAT TCTGATATTC AAACCAAAAA GGAATATGGA 
GTTTCAAATG AACACAAACA GAAAGGCAAA GTTAAAAGAA AATTAAAGAA CCAGAACAAA 
AATAAAGAGA ACCAAGAGCT GAAGCAAGAA AAAGAGGGGA AAGAAAATAC CAGAGTAACA 
AACTTGACAG TTAATACTGC ACTAGATTGT TCAGATAAGA CCAGAGATGA GGGGAATTTT 
AGGAAATCTT TTAGCCCGAA AGATGATACT TCATTACATT TATTTCATAT TTCCAGTGGT 
AAATCTCCCA AACATTCTTG TGGACTCAGT GAAAAGCAGT CAACACCACT AAAACAAGAA 
CATTCTAAAA CCTGTTTATC ACCAGGAAGT TCTGAAATGT CATTACAGCC TGATCTTGTT 
CGGTATGATA ATATAGAATC TGAATTCTTG CCAGAAAGTT CAAGTGTAAA ATCTTGTAAG 
CATAAGGAAA AAAACAAACA TCATAAAGAT TTCCACTTAG AATTTGGTGA AAAGTCAAAT 
GCCAAAATAA AAGATGATGA TCACAGTCCA ACATTTGAAA ATTCAGATTG CACACTGAAA 
AAAATGGATA AAGAGGGTAA AACATTAAAA AAACATAAGT TAAAACATAA AGAAAGGGAA 
AAAGAAAAGC ATAAAAAAGA AATTGAAGGT GAAAAGGAAA AATACAAAAG TAGGGATGGT 
ACTAAAGAGC TGCAACGGAG TGTGGAATTT GATAGAGAGT TTTGGAAAGA GAATTTTTTT 
AAAAGTGATG AAACCGAAGA TCTATTTTTA AGTATGGAAC ATGAGTCCCT AACGTTAGAA 
AAAAAGTCAA AGTTGGAGAA AAACATAAAA GATGATAAAT CAACTAAGGA AAAGCATATC 
TCCAAAGAGA GAAACTTTAA AGAAGAACGG GAAAAGATTA AAAAGGAAAG TGAGAAATCT 
TTTAGGGAGG AAAAAATAAA AGATTTAAAA GATGACAGAG AGAACGTACT TACAGATAAA 
GAAACAGAAT TCAGCTCTTT AGGTGTAAGT GCCACTGAAG AGACTATAGG GTTACATTCA 
GTGGAAAAGG AAATAGAAAT TGAAAAGCAT ATGAAAGAAA GTAAGGAAAA ATCTGAGAAA 
CGATTTCAAA CTAAAGATAA GGACATTGAG AGGGCTGACA GAAAAAATTC TGAAAAAGAG 
AAGAAGATAA AACATGAGCA TAAGTCAGAG AAAGACAGAT TAGATCCTAG TGAATGTGTC 
GACAGAATAA AAGAAAAGGA CAAGCTATAT TCACATCACA CAGAAAAATG CCATAGAGAA 
GGTGAGAAGC TAAAAAACAT TACTACCATT AAAAAAAACG ATGACAGAGA TAAAAGTAGA 
GAAAAGATGG ATAGGAAACA TGACAAAGAA AAACCTGAGA AGGAGAGACA TCCAGTAGAG 
AGCAAAGAGA AGCACTTGAT GGAAAAAAAA AACAAGCCAT CAGATAATAG TGACTATACT 
AAATCAGAAA AAAGCAAAAA TAAAGAAAAA GATAGGGAGG TAGATAAAAA GGACAAATCT 
AGAGACAGTG TAAATATAAG TAATTCCAAA CACTTCCAGG AAGAGAGAAA GTCAAGTATA 
ACAGACAGTA ATAAAGCACA ACATGAAAAA ACTCTCTCTC TCAAAGAAAA AACAAAAGAT 
GAACCTTTGA GAACTCCTGA TGGAAAAGAA AAAGATAAAA AAGATATAGA TAGATATAAA 
GAACGAGACA AACATAAGGA TAAAATTCTG AGTAGTTCAC TCAAACTAAA ATCTGAAGCA 
GATAAGCCTA AACCGAAGTC ATCACCAGCA TCAAAAGATA CTCGGCCCAA AGAAAAGAGG 
CTTGTGAATG ACGACTTAAT GCAGACCAGC TTTGAACGGA TGCTGAGCCT TAAAGACTTG 
GAAATAGAGC AGTGGCACAG AAAACACAAG GAAAAGATTA AGCAAAAAGA AAAGGAACGG 
TTGAGAAATC GCAGTTGTTT AGAACTTAAA GTAAAAGATA AAGAAAAAAC AAAGCATGCA 
CTAGCTGAGT CCAAAAATAA AGAACTTACT AGATCAAAGA GTTCTGAGTT GACTGATACT 
TACATTAAGG AGAAACACTG TAAAGATGCT GTAAGTAATA GATCCCAATC TGTGGACACC 
AAAAATACGA TGAGTTTAGG CAAGTCATCC TTTGTTTCAG ATAATAGCTT GAACAGGTCT 
CCTAGATCAG AAAGTGAAAA GCCAGGTCTC AGCTCCAGGT CTGTCTCCTT GATTTCAGTG 
GCTAGCTCAG AAGATTCCTG CCTTACTACA ATGACAACCC CAAGGCCTCC AGTTGAGTAT 
GACTCAGACT TTATGTTAGA GGGTTCGGAT TCCCAAATGT CCTTTTCCCA GTCACCGTTT 
TTGCCAGTTG CCAAATCTCC TGCCCTTCAT GACAGGGAGC TGGATAGCCT GACTGATCTA 
TCAGAGCGGA TTAAAGCATC GTATGCTAGC AGGCTTCTGA CCTCCCATCT CCGGGCATCT 
TCTGTCGAAG ATGCTAAACT AGTTACAAAT GAAGGGAGAT CAGCTGCAGA AGCCCGAAGA 
TGTAGCATGC CCTCTGTCAT TTGTGAGCAT ACAAAGCAAT TCCAAACAAT ATCAGAAGAG 
GGCAGTCAAG ATGGCTCAGC TGTGCCAAGA GAGATTTGTC CTTCTCCCAA ATCTGAGGTA 
TCCTCCAACG TGCCTGAAAG AGAACTTTTG AGCGTGTCCA ACATACATTC CAGTTTTGCA 
GCCTCTCCAA CTAGATCTAT AAACAGCAAA TATAATTCTA CTGATACAAA TGTCATCAAG 
GGCACTGCTC CCTTGGGCAT GCTGATGGGC AGTCCTGTGC ACTTAGAGTC CTCTAATCAG 
GTTGGTGTGA TCCAAAGTAA GTCATGGGAG ATGGAGTCGT TAAACACCGG AGACTTTAGC 
TGCCCAAATT CTGCTGCACC TGATCAAGAT TCTTCTGTTC AGGGTTTTTG TAATCCTGAA 
AACAAAGTAT TGAGAGAACA GAATATTGAT TTTGTGTCCA TGCACCAGAC TGAATTGCCG 
GCAAACACTT GTGCTCAGGA TCCAGCGTCC GTCCTTCCTT CCCAGCAACC TTGCTCTTCG 
CATAGCCAAT CACTTTCAGA TGCTGAATCT ATTCCCAGAC ATACTTCTTT GTCCTATGTC 
ACCACTCAAG AACCAGGTAT TCTACAGCAG AAAAATGCAA TTCAAATGAT CAGTTCTGTT 
TTGGATACTG ATACTGAACC TACAAAAGAT ATGGAAAATA CTTTTGTCCT AACAGACGTT 
CAGAAGACAG ATGCCTTTGT CCCAGTGTAC TCTGAAAGCA CTGTTGAAGA AGCAGCACCA 
AATTTTGAGA AAGCTAGTAC TTTGCCAGTA TTGCCATCAG AAAAGGACTT TAATGGAAGT 
GACGGGTCTT CCCAGCCAAA TCCTCATTAT GCATTTAGCA AGCTAATGTT TAAGTCTTCC 
AGTGGCCATG AGGCTGAAAA AAGCACTTCT GATACTCAGG ATACTTCACA TGAAAAGGAA 
AACAAACTGG AGAGTTTGGT TTTGACTCAC TTAAATGATA AGTGTGATTC TGATTTATGT 
GAAATGAATG TAGGGGTGCC AAAAGGAAAC CTGAGTGAAC CAGATAATCC AAAACATTGT 
CCTGAAAGTG AGAAGTGTTT GCTTTCCATC GAGGATGAGG AGTCACAACA AAGTACTTTA 
TCGAGTCTGG AGAACCATCC ACAGCCATCG GCTCCACCAG AAATGCATAA ATACGGACAC 
TTAGTTAAAG TAGAACTAGA GGAAAGTGCT GAAGATGATA AACCTGAAAG CCAGATCCCT 
CAAAGGATGA CCAGAAACAA AGGAAACACA GGAGCCAGTC AAAGCAAGCA GCTTCTGGCC 
AGCTGTGCAC TGTTGCCAGA CAAAGACAGT GAGTCTGCGT CCCCTCGAGG AAGAATAAGA 
TTAACTGAGG AGGACGACCC TCAGATTCAC CACCCGCGGA AAAGGAAAGT GTCCCGGGTG 
CCTCAGCCGG TGCAAGTGAG TCCTTCTCTG CTGCAAGCAA AAGAGAAAAC CCAGCAGTCC 
CTGGCCGCCA TTGTAGACTC GCTGAAGCTC GATGAGATTC AGCCATACAG CTCAGAGCGC 
GCAAATCCCT ATTTTGAATA CTTGCACATA AGAAAAAAGA TTGAAGAAAA GCGCAAGTTG 
TTATGTAGTG TGATCCCTCA GGCACCTCAG TATTATGATG AATACGTGAC GTTTAATGGG 
TCGTATCTCC TGGACGGAAA CCCCTTAAGC AAGATCTGCA TACCCACAAT TACACCCCCA 
CCTTCACTGT CAGATCCACT TAAAGAGCTT TTTCGACAAC AGGAAGTTGT CAGGATGAAA 
CTACGTTTGC AGCACAGTAT TGAAAGGGAA AAACTCATTG TATCCAATGA ACAGGAGGTT 
CTTCGGGTTC ATTACAGAGC TGCAAGGACA CTGGCAAATC AGACACTGCC ATTCAGCGCA 
TGCACGGTTC TCCTGGATGC GGAAGTGTAC AGCGTGCCCC TGGATGCCCA GTCTGATGAC 
AGTAAAACTT CTGTGAGGGA TCGCTTTAAT GCAAGACAGT TCATGTCATG GTTACAAGAT 
GTGGATGATA AATTCGACAA ATTAAAGACC TGTCTTTTAA TGAGGCAACA GCATGAAGCT 
GCAGCTTTAA ATGCCGTCCA GAGGTTAGAA TGGCAGCTCA AACTCCAGGA ACTTGATCCT 
GCCACCTATA AATCTATCAG CATCTATGAA ATTCAGGAGT TTTACGTTCC GCTGGTTGAT 
GTTAACGATG ACTTTGAATT GACTCCTATA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697790.1
CDS	221..6373
Translation	MPKSGFTKPVQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIDRNDVGKEMKEKSSMKRKLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTCSEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTPPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQIVPSKAPLPSALDEYEFKDDDEEEINKMIDDRHILRKELRKENESEVEKSSLFAKQEKTFYPKSFKSKKQKPSRVLYSSSESSDEEILQNKKCSAPCSVPETSNSDIQTKKEYGVSNEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRVTNLTVNTALDCSDKTRDEGNFRKSFSPKDDTSLHLFHISSGKSPKHSCGLSEKQSTPLKQEHSKTCLSPGSSEMSLQPDLVRYDNIESEFLPESSSVKSCKHKEKNKHHKDFHLEFGEKSNAKIKDDDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKSRDGTKELQRSVEFDREFWKENFFKSDETEDLFLSMEHESLTLEKKSKLEKNIKDDKSTKEKHISKERNFKEEREKIKKESEKSFREEKIKDLKDDRENVLTDKETEFSSLGVSATEETIGLHSVEKEIEIEKHMKESKEKSEKRFQTKDKDIERADRKNSEKEKKIKHEHKSEKDRLDPSECVDRIKEKDKLYSHHTEKCHREGEKLKNITTIKKNDDRDKSREKMDRKHDKEKPEKERHPVESKEKHLMEKKNKPSDNSDYTKSEKSKNKEKDREVDKKDKSRDSVNISNSKHFQEERKSSITDSNKAQHEKTLSLKEKTKDEPLRTPDGKEKDKKDIDRYKERDKHKDKILSSSLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRSCLELKVKDKEKTKHALAESKNKELTRSKSSELTDTYIKEKHCKDAVSNRSQSVDTKNTMSLGKSSFVSDNSLNRSPRSESEKPGLSSRSVSLISVASSEDSCLTTMTTPRPPVEYDSDFMLEGSDSQMSFSQSPFLPVAKSPALHDRELDSLTDLSERIKASYASRLLTSHLRASSVEDAKLVTNEGRSAAEARRCSMPSVICEHTKQFQTISEEGSQDGSAVPREICPSPKSEVSSNVPERELLSVSNIHSSFAASPTRSINSKYNSTDTNVIKGTAPLGMLMGSPVHLESSNQVGVIQSKSWEMESLNTGDFSCPNSAAPDQDSSVQGFCNPENKVLREQNIDFVSMHQTELPANTCAQDPASVLPSQQPCSSHSQSLSDAESIPRHTSLSYVTTQEPGILQQKNAIQMISSVLDTDTEPTKDMENTFVLTDVQKTDAFVPVYSESTVEEAAPNFEKASTLPVLPSEKDFNGSDGSSQPNPHYAFSKLMFKSSSGHEAEKSTSDTQDTSHEKENKLESLVLTHLNDKCDSDLCEMNVGVPKGNLSEPDNPKHCPESEKCLLSIEDEESQQSTLSSLENHPQPSAPPEMHKYGHLVKVELEESAEDDKPESQIPQRMTRNKGNTGASQSKQLLASCALLPDKDSESASPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYSVPLDAQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_002697744.5
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697744.5

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGATTCA 
GACCCAGGAC ATACAAGTGA GAATTGGGGG GAGAGACTTA TATCTTCTTA CAGGACATGC 
TCAGAGAAAG AAGGTCCAGA AAAGAAGAAA ACAAAAAAGG AAGCTGGAAA TAAGAAGTCC 
ACTCCAGTTA GCATTCTCTT TGGTTACCCA CTCTCAGAGC GAAAGCAGAT GGCACTTCTT 
ATGCAGATGA CAGCACGGGA CAACAGTCCA GATTCCACAC CAAATCATCC ATCGCAAACA 
CCACCAGCCC AAAAGAAAAC TCCCAGTTCC TCATCTCGAC AAAAAGATAA AGTTAATAAA 
AGAAATGAAC GTGGTGAAAC TCCTTTACAC ATGGCAGCTA TTCGAGGAGA TGTAAAACAA 
GTCAAAGAAC TAATAAGTTT AGGGGCAAAT GTGAATGTGA AAGATTTTGC AGGTTGGACA 
CCACTGCATG AAGCTTGCAA CGTGGGATAT TATGATGTTG CTAAGATACT GATAGCAGCT 
GGAGCAGATG TTAACACACA AGGATTAGAT GATGACACTC CACTCCATGA TTCCGCTAGT 
AGTGGGCACA GAGATATTGT GAAACTGCTG CTTCGTCATG GTGGAAACCC TTTTCAAGCT 
AATAAACATG GGGAGCGTCC AGTAGATGTG GCAGAAACAG AGGAGCTGGA GTTGTTGCTG 
AAAAGAGAGG TGCCTTTGTC TGATGATGAT GAGAGTTACA CAGATTCTGA AGAGGCTCAG 
TCTGTAAATC CTTCCAGCGT TGATGAAAAT ATTGACTCTG AGACAGAGAA AGACTCTCTC 
ATCTGTGAAA GTAAACAGAT AGTTCCCAGT AAAGCACCTC TTCCATCTGC CCTTGATGAG 
TATGAGTTCA AAGATGATGA TGAAGAGGAA ATAAATAAAA TGATTGATGA CAGGCATATT 
CTTAGGAAAG AACTGCGAAA AGAGAATGAA TCTGAAGTAG AAAAGAGTAG TTTATTTGCA 
AAACAGGAAA AAACCTTCTA TCCTAAATCA TTTAAAAGTA AAAAACAAAA GCCATCTAGG 
GTTTTGTATT CAAGTTCTGA AAGTTCAGAT GAAGAAATTC TTCAAAACAA AAAGTGTTCT 
GCTCCATGTT CTGTCCCTGA AACATCAAAT TCTGATATTC AAACCAAAAA GGAATATGGA 
GTTTCAAATG AACACAAACA GAAAGGCAAA GTTAAAAGAA AATTAAAGAA CCAGAACAAA 
AATAAAGAGA ACCAAGAGCT GAAGCAAGAA AAAGAGGGGA AAGAAAATAC CAGAGTAACA 
AACTTGACAG TTAATACTGC ACTAGATTGT TCAGATAAGA CCAGAGATGA GGGGAATTTT 
AGGAAATCTT TTAGCCCGAA AGATGATACT TCATTACATT TATTTCATAT TTCCAGTGGT 
AAATCTCCCA AACATTCTTG TGGACTCAGT GAAAAGCAGT CAACACCACT AAAACAAGAA 
CATTCTAAAA CCTGTTTATC ACCAGGAAGT TCTGAAATGT CATTACAGCC TGATCTTGTT 
CGGTATGATA ATATAGAATC TGAATTCTTG CCAGAAAGTT CAAGTGTAAA ATCTTGTAAG 
CATAAGGAAA AAAACAAACA TCATAAAGAT TTCCACTTAG AATTTGGTGA AAAGTCAAAT 
GCCAAAATAA AAGATGATGA TCACAGTCCA ACATTTGAAA ATTCAGATTG CACACTGAAA 
AAAATGGATA AAGAGGGTAA AACATTAAAA AAACATAAGT TAAAACATAA AGAAAGGGAA 
AAAGAAAAGC ATAAAAAAGA AATTGAAGGT GAAAAGGAAA AATACAAAAG TAGGGATGGT 
ACTAAAGAGC TGCAACGGAG TGTGGAATTT GATAGAGAGT TTTGGAAAGA GAATTTTTTT 
AAAAGTGATG AAACCGAAGA TCTATTTTTA AGTATGGAAC ATGAGTCCCT AACGTTAGAA 
AAAAAGTCAA AGTTGGAGAA AAACATAAAA GATGATAAAT CAACTAAGGA AAAGCATATC 
TCCAAAGAGA GAAACTTTAA AGAAGAACGG GAAAAGATTA AAAAGGAAAG TGAGAAATCT 
TTTAGGGAGG AAAAAATAAA AGATTTAAAA GATGACAGAG AGAACGTACT TACAGATAAA 
GAAACAGAAT TCAGCTCTTT AGGTGTAAGT GCCACTGAAG AGACTATAGG GTTACATTCA 
GTGGAAAAGG AAATAGAAAT TGAAAAGCAT ATGAAAGAAA GTAAGGAAAA ATCTGAGAAA 
CGATTTCAAA CTAAAGATAA GGACATTGAG AGGGCTGACA GAAAAAATTC TGAAAAAGAG 
AAGAAGATAA AACATGAGCA TAAGTCAGAG AAAGACAGAT TAGATCCTAG TGAATGTGTC 
GACAGAATAA AAGAAAAGGA CAAGCTATAT TCACATCACA CAGAAAAATG CCATAGAGAA 
GGTGAGAAGC TAAAAAACAT TACTACCATT AAAAAAAACG ATGACAGAGA TAAAAGTAGA 
GAAAAGATGG ATAGGAAACA TGACAAAGAA AAACCTGAGA AGGAGAGACA TCCAGTAGAG 
AGCAAAGAGA AGCACTTGAT GGAAAAAAAA AACAAGCCAT CAGATAATAG TGACTATACT 
AAATCAGAAA AAAGCAAAAA TAAAGAAAAA GATAGGGAGG TAGATAAAAA GGACAAATCT 
AGAGACAGTG TAAATATAAG TAATTCCAAA CACTTCCAGG AAGAGAGAAA GTCAAGTATA 
ACAGACAGTA ATAAAGCACA ACATGAAAAA ACTCTCTCTC TCAAAGAAAA AACAAAAGAT 
GAACCTTTGA GAACTCCTGA TGGAAAAGAA AAAGATAAAA AAGATATAGA TAGATATAAA 
GAACGAGACA AACATAAGGA TAAAATTCTG AGTAGTTCAC TCAAACTAAA ATCTGAAGCA 
GATAAGCCTA AACCGAAGTC ATCACCAGCA TCAAAAGATA CTCGGCCCAA AGAAAAGAGG 
CTTGTGAATG ACGACTTAAT GCAGACCAGC TTTGAACGGA TGCTGAGCCT TAAAGACTTG 
GAAATAGAGC AGTGGCACAG AAAACACAAG GAAAAGATTA AGCAAAAAGA AAAGGAACGG 
TTGAGAAATC GCAGTTGTTT AGAACTTAAA GTAAAAGATA AAGAAAAAAC AAAGCATGCA 
CTAGCTGAGT CCAAAAATAA AGAACTTACT AGATCAAAGA GTTCTGAGTT GACTGATACT 
TACATTAAGG AGAAACACTG TAAAGATGCT GTAAGTAATA GATCCCAATC TGTGGACACC 
AAAAATACGA TGAGTTTAGG CAAGTCATCC TTTGTTTCAG ATAATAGCTT GAACAGGTCT 
CCTAGATCAG AAAGTGAAAA GCCAGGTCTC AGCTCCAGGT CTGTCTCCTT GATTTCAGTG 
GCTAGCTCAG AAGATTCCTG CCTTACTACA ATGACAACCC CAAGGCCTCC AGTTGAGTAT 
GACTCAGACT TTATGTTAGA GGGTTCGGAT TCCCAAATGT CCTTTTCCCA GTCACCGTTT 
TTGCCAGTTG CCAAATCTCC TGCCCTTCAT GACAGGGAGC TGGATAGCCT GACTGATCTA 
TCAGAGCGGA TTAAAGCATC GTATGCTAGC AGGCTTCTGA CCTCCCATCT CCGGGCATCT 
TCTGTCGAAG ATGCTAAACT AGTTACAAAT GAAGGGAGAT CAGCTGCAGA AGCCCGAAGA 
TGTAGCATGC CCTCTGTCAT TTGTGAGCAT ACAAAGCAAT TCCAAACAAT ATCAGAAGAG 
GGCAGTCAAG ATGGCTCAGC TGTGCCAAGA GAGATTTGTC CTTCTCCCAA ATCTGAGGTA 
TCCTCCAACG TGCCTGAAAG AGAACTTTTG AGCGTGTCCA ACATACATTC CAGTTTTGCA 
GCCTCTCCAA CTAGATCTAT AAACAGCAAA TATAATTCTA CTGATACAAA TGTCATCAAG 
GGCACTGCTC CCTTGGGCAT GCTGATGGGC AGTCCTGTGC ACTTAGAGTC CTCTAATCAG 
GTTGGTGTGA TCCAAAGTAA GTCATGGGAG ATGGAGTCGT TAAACACCGG AGACTTTAGC 
TGCCCAAATT CTGCTGCACC TGATCAAGAT TCTTCTGTTC AGGGTTTTTG TAATCCTGAA 
AACAAAGTAT TGAGAGAACA GAATATTGAT TTTGTGTCCA TGCACCAGAC TGAATTGCCG 
GCAAACACTT GTGCTCAGGA TCCAGCGTCC GTCCTTCCTT CCCAGCAACC TTGCTCTTCG 
CATAGCCAAT CACTTTCAGA TGCTGAATCT ATTCCCAGAC ATACTTCTTT GTCCTATGTC 
ACCACTCAAG AACCAGGTAT TCTACAGCAG AAAAATGCAA TTCAAATGAT CAGTTCTGTT 
TTGGATACTG ATACTGAACC TACAAAAGAT ATGGAAAATA CTTTTGTCCT AACAGACGTT 
CAGAAGACAG ATGCCTTTGT CCCAGTGTAC TCTGAAAGCA CTGTTGAAGA AGCAGCACCA 
AATTTTGAGA AAGCTAGTAC TTTGCCAGTA TTGCCATCAG AAAAGGACTT TAATGGAAGT 
GACGGGTCTT CCCAGCCAAA TCCTCATTAT GCATTTAGCA AGCTAATGTT TAAGTCTTCC 
AGTGGCCATG AGGCTGAAAA AAGCACTTCT GATACTCAGG ATACTTCACA TGAAAAGGAA 
AACAAACTGG AGAGTTTGGT TTTGACTCAC TTAAATGATA AGTGTGATTC TGATTTATGT 
GAAATGAATG TAGGGGTGCC AAAAGGAAAC CTGAGTGAAC CAGATAATCC AAAACATTGT 
CCTGAAAGTG AGAAGTGTTT GCTTTCCATC GAGGATGAGG AGTCACAACA AAGTACTTTA 
TCGAGTCTGG AGAACCATCC ACAGCCATCG GCTCCACCAG AAATGCATAA ATACGGACAC 
TTAGTTAAAG TAGAACTAGA GGAAAGTGCT GAAGATGATA AACCTGAAAG CCAGATCCCT 
CAAAGGATGA CCAGAAACAA AGGAAACACA GGAGCCAGTC AAAGCAAGCA GCTTCTGGCC 
AGCTGTGCAC TGTTGCCAGA CAAAGACAGT GAGTCTGCGT CCCCTCGAGG AAGAATAAGA 
TTAACTGAGG AGGACGACCC TCAGATTCAC CACCCGCGGA AAAGGAAAGT GTCCCGGGTG 
CCTCAGCCGG TGCAAGTGAG TCCTTCTCTG CTGCAAGCAA AAGAGAAAAC CCAGCAGTCC 
CTGGCCGCCA TTGTAGACTC GCTGAAGCTC GATGAGATTC AGCCATACAG CTCAGAGCGC 
GCAAATCCCT ATTTTGAATA CTTGCACATA AGAAAAAAGA TTGAAGAAAA GCGCAAGTTG 
TTATGTAGTG TGATCCCTCA GGCACCTCAG TATTATGATG AATACGTGAC GTTTAATGGG 
TCGTATCTCC TGGACGGAAA CCCCTTAAGC AAGATCTGCA TACCCACAAT TACACCCCCA 
CCTTCACTGT CAGATCCACT TAAAGAGCTT TTTCGACAAC AGGAAGTTGT CAGGATGAAA 
CTACGTTTGC AGCACAGTAT TGAAAGGGAA AAACTCATTG TATCCAATGA ACAGGAGGTT 
CTTCGGGTTC ATTACAGAGC TGCAAGGACA CTGGCAAATC AGACACTGCC ATTCAGCGCA 
TGCACGGTTC TCCTGGATGC GGAAGTGTAC AGCGTGCCCC TGGATGCCCA GTCTGATGAC 
AGTAAAACTT CTGTGAGGGA TCGCTTTAAT GCAAGACAGT TCATGTCATG GTTACAAGAT 
GTGGATGATA AATTCGACAA ATTAAAGACC TGTCTTTTAA TGAGGCAACA GCATGAAGCT 
GCAGCTTTAA ATGCCGTCCA GAGGTTAGAA TGGCAGCTCA AACTCCAGGA ACTTGATCCT 
GCCACCTATA AATCTATCAG CATCTATGAA ATTCAGGAGT TTTACGTTCC GCTGGTTGAT 
GTTAACGATG ACTTTGAATT GACTCCTATA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_002697743.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697789.1 Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X2, mRNA.

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CloneID	OBa11573
Clone ID Related Accession (Same CDS sequence)	XM_002697743.4 , XM_015469151.1 , XM_024984610.1 , XM_002697743.5
Accession Version	XM_002697743.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002697743.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA AACAAAAAAG GAAGCTGGAA ATAAGAAGTC CACTCCAGTT 
AGCATTCTCT TTGGTTACCC ACTCTCAGAG CGAAAGCAGA TGGCACTTCT TATGCAGATG 
ACAGCACGGG ACAACAGTCC AGATTCCACA CCAAATCATC CATCGCAAAC ACCACCAGCC 
CAAAAGAAAA CTCCCAGTTC CTCATCTCGA CAAAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCAGCT ATTCGAGGAG ATGTAAAACA AGTCAAAGAA 
CTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ACGTGGGATA TTATGATGTT GCTAAGATAC TGATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCCGCTAG TAGTGGGCAC 
AGAGATATTG TGAAACTGCT GCTTCGTCAT GGTGGAAACC CTTTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTAGATGT GGCAGAAACA GAGGAGCTGG AGTTGTTGCT GAAAAGAGAG 
GTGCCTTTGT CTGATGATGA TGAGAGTTAC ACAGATTCTG AAGAGGCTCA GTCTGTAAAT 
CCTTCCAGCG TTGATGAAAA TATTGACTCT GAGACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TAGTTCCCAG TAAAGCACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGAAGAGGA AATAAATAAA ATGATTGATG ACAGGCATAT TCTTAGGAAA 
GAACTGCGAA AAGAGAATGA ATCTGAAGTA GAAAAGAGTA GTTTATTTGC AAAACAGGAA 
AAAACCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTTTTGTAT 
TCAAGTTCTG AAAGTTCAGA TGAAGAAATT CTTCAAAACA AAAAGTGTTC TGCTCCATGT 
TCTGTCCCTG AAACATCAAA TTCTGATATT CAAACCAAAA AGGAATATGG AGTTTCAAAT 
GAACACAAAC AGAAAGGCAA AGTTAAAAGA AAATTAAAGA ACCAGAACAA AAATAAAGAG 
AACCAAGAGC TGAAGCAAGA AAAAGAGGGG AAAGAAAATA CCAGAGTAAC AAACTTGACA 
GTTAATACTG CACTAGATTG TTCAGATAAG ACCAGAGATG AGGGGAATTT TAGGAAATCT 
TTTAGCCCGA AAGATGATAC TTCATTACAT TTATTTCATA TTTCCAGTGG TAAATCTCCC 
AAACATTCTT GTGGACTCAG TGAAAAGCAG TCAACACCAC TAAAACAAGA ACATTCTAAA 
ACCTGTTTAT CACCAGGAAG TTCTGAAATG TCATTACAGC CTGATCTTGT TCGGTATGAT 
AATATAGAAT CTGAATTCTT GCCAGAAAGT TCAAGTGTAA AATCTTGTAA GCATAAGGAA 
AAAAACAAAC ATCATAAAGA TTTCCACTTA GAATTTGGTG AAAAGTCAAA TGCCAAAATA 
AAAGATGATG ATCACAGTCC AACATTTGAA AATTCAGATT GCACACTGAA AAAAATGGAT 
AAAGAGGGTA AAACATTAAA AAAACATAAG TTAAAACATA AAGAAAGGGA AAAAGAAAAG 
CATAAAAAAG AAATTGAAGG TGAAAAGGAA AAATACAAAA GTAGGGATGG TACTAAAGAG 
CTGCAACGGA GTGTGGAATT TGATAGAGAG TTTTGGAAAG AGAATTTTTT TAAAAGTGAT 
GAAACCGAAG ATCTATTTTT AAGTATGGAA CATGAGTCCC TAACGTTAGA AAAAAAGTCA 
AAGTTGGAGA AAAACATAAA AGATGATAAA TCAACTAAGG AAAAGCATAT CTCCAAAGAG 
AGAAACTTTA AAGAAGAACG GGAAAAGATT AAAAAGGAAA GTGAGAAATC TTTTAGGGAG 
GAAAAAATAA AAGATTTAAA AGATGACAGA GAGAACGTAC TTACAGATAA AGAAACAGAA 
TTCAGCTCTT TAGGTGTAAG TGCCACTGAA GAGACTATAG GGTTACATTC AGTGGAAAAG 
GAAATAGAAA TTGAAAAGCA TATGAAAGAA AGTAAGGAAA AATCTGAGAA ACGATTTCAA 
ACTAAAGATA AGGACATTGA GAGGGCTGAC AGAAAAAATT CTGAAAAAGA GAAGAAGATA 
AAACATGAGC ATAAGTCAGA GAAAGACAGA TTAGATCCTA GTGAATGTGT CGACAGAATA 
AAAGAAAAGG ACAAGCTATA TTCACATCAC ACAGAAAAAT GCCATAGAGA AGGTGAGAAG 
CTAAAAAACA TTACTACCAT TAAAAAAAAC GATGACAGAG ATAAAAGTAG AGAAAAGATG 
GATAGGAAAC ATGACAAAGA AAAACCTGAG AAGGAGAGAC ATCCAGTAGA GAGCAAAGAG 
AAGCACTTGA TGGAAAAAAA AAACAAGCCA TCAGATAATA GTGACTATAC TAAATCAGAA 
AAAAGCAAAA ATAAAGAAAA AGATAGGGAG GTAGATAAAA AGGACAAATC TAGAGACAGT 
GTAAATATAA GTAATTCCAA ACACTTCCAG GAAGAGAGAA AGTCAAGTAT AACAGACAGT 
AATAAAGCAC AACATGAAAA AACTCTCTCT CTCAAAGAAA AAACAAAAGA TGAACCTTTG 
AGAACTCCTG ATGGAAAAGA AAAAGATAAA AAAGATATAG ATAGATATAA AGAACGAGAC 
AAACATAAGG ATAAAATTCT GAGTAGTTCA CTCAAACTAA AATCTGAAGC AGATAAGCCT 
AAACCGAAGT CATCACCAGC ATCAAAAGAT ACTCGGCCCA AAGAAAAGAG GCTTGTGAAT 
GACGACTTAA TGCAGACCAG CTTTGAACGG ATGCTGAGCC TTAAAGACTT GGAAATAGAG 
CAGTGGCACA GAAAACACAA GGAAAAGATT AAGCAAAAAG AAAAGGAACG GTTGAGAAAT 
CGCAGTTGTT TAGAACTTAA AGTAAAAGAT AAAGAAAAAA CAAAGCATGC ACTAGCTGAG 
TCCAAAAATA AAGAACTTAC TAGATCAAAG AGTTCTGAGT TGACTGATAC TTACATTAAG 
GAGAAACACT GTAAAGATGC TGTAAGTAAT AGATCCCAAT CTGTGGACAC CAAAAATACG 
ATGAGTTTAG GCAAGTCATC CTTTGTTTCA GATAATAGCT TGAACAGGTC TCCTAGATCA 
GAAAGTGAAA AGCCAGGTCT CAGCTCCAGG TCTGTCTCCT TGATTTCAGT GGCTAGCTCA 
GAAGATTCCT GCCTTACTAC AATGACAACC CCAAGGCCTC CAGTTGAGTA TGACTCAGAC 
TTTATGTTAG AGGGTTCGGA TTCCCAAATG TCCTTTTCCC AGTCACCGTT TTTGCCAGTT 
GCCAAATCTC CTGCCCTTCA TGACAGGGAG CTGGATAGCC TGACTGATCT ATCAGAGCGG 
ATTAAAGCAT CGTATGCTAG CAGGCTTCTG ACCTCCCATC TCCGGGCATC TTCTGTCGAA 
GATGCTAAAC TAGTTACAAA TGAAGGGAGA TCAGCTGCAG AAGCCCGAAG ATGTAGCATG 
CCCTCTGTCA TTTGTGAGCA TACAAAGCAA TTCCAAACAA TATCAGAAGA GGGCAGTCAA 
GATGGCTCAG CTGTGCCAAG AGAGATTTGT CCTTCTCCCA AATCTGAGGT ATCCTCCAAC 
GTGCCTGAAA GAGAACTTTT GAGCGTGTCC AACATACATT CCAGTTTTGC AGCCTCTCCA 
ACTAGATCTA TAAACAGCAA ATATAATTCT ACTGATACAA ATGTCATCAA GGGCACTGCT 
CCCTTGGGCA TGCTGATGGG CAGTCCTGTG CACTTAGAGT CCTCTAATCA GGTTGGTGTG 
ATCCAAAGTA AGTCATGGGA GATGGAGTCG TTAAACACCG GAGACTTTAG CTGCCCAAAT 
TCTGCTGCAC CTGATCAAGA TTCTTCTGTT CAGGGTTTTT GTAATCCTGA AAACAAAGTA 
TTGAGAGAAC AGAATATTGA TTTTGTGTCC ATGCACCAGA CTGAATTGCC GGCAAACACT 
TGTGCTCAGG ATCCAGCGTC CGTCCTTCCT TCCCAGCAAC CTTGCTCTTC GCATAGCCAA 
TCACTTTCAG ATGCTGAATC TATTCCCAGA CATACTTCTT TGTCCTATGT CACCACTCAA 
GAACCAGGTA TTCTACAGCA GAAAAATGCA ATTCAAATGA TCAGTTCTGT TTTGGATACT 
GATACTGAAC CTACAAAAGA TATGGAAAAT ACTTTTGTCC TAACAGACGT TCAGAAGACA 
GATGCCTTTG TCCCAGTGTA CTCTGAAAGC ACTGTTGAAG AAGCAGCACC AAATTTTGAG 
AAAGCTAGTA CTTTGCCAGT ATTGCCATCA GAAAAGGACT TTAATGGAAG TGACGGGTCT 
TCCCAGCCAA ATCCTCATTA TGCATTTAGC AAGCTAATGT TTAAGTCTTC CAGTGGCCAT 
GAGGCTGAAA AAAGCACTTC TGATACTCAG GATACTTCAC ATGAAAAGGA AAACAAACTG 
GAGAGTTTGG TTTTGACTCA CTTAAATGAT AAGTGTGATT CTGATTTATG TGAAATGAAT 
GTAGGGGTGC CAAAAGGAAA CCTGAGTGAA CCAGATAATC CAAAACATTG TCCTGAAAGT 
GAGAAGTGTT TGCTTTCCAT CGAGGATGAG GAGTCACAAC AAAGTACTTT ATCGAGTCTG 
GAGAACCATC CACAGCCATC GGCTCCACCA GAAATGCATA AATACGGACA CTTAGTTAAA 
GTAGAACTAG AGGAAAGTGC TGAAGATGAT AAACCTGAAA GCCAGATCCC TCAAAGGATG 
ACCAGAAACA AAGGAAACAC AGGAGCCAGT CAAAGCAAGC AGCTTCTGGC CAGCTGTGCA 
CTGTTGCCAG ACAAAGACAG TGAGTCTGCG TCCCCTCGAG GAAGAATAAG ATTAACTGAG 
GAGGACGACC CTCAGATTCA CCACCCGCGG AAAAGGAAAG TGTCCCGGGT GCCTCAGCCG 
GTGCAAGTGA GTCCTTCTCT GCTGCAAGCA AAAGAGAAAA CCCAGCAGTC CCTGGCCGCC 
ATTGTAGACT CGCTGAAGCT CGATGAGATT CAGCCATACA GCTCAGAGCG CGCAAATCCC 
TATTTTGAAT ACTTGCACAT AAGAAAAAAG ATTGAAGAAA AGCGCAAGTT GTTATGTAGT 
GTGATCCCTC AGGCACCTCA GTATTATGAT GAATACGTGA CGTTTAATGG GTCGTATCTC 
CTGGACGGAA ACCCCTTAAG CAAGATCTGC ATACCCACAA TTACACCCCC ACCTTCACTG 
TCAGATCCAC TTAAAGAGCT TTTTCGACAA CAGGAAGTTG TCAGGATGAA ACTACGTTTG 
CAGCACAGTA TTGAAAGGGA AAAACTCATT GTATCCAATG AACAGGAGGT TCTTCGGGTT 
CATTACAGAG CTGCAAGGAC ACTGGCAAAT CAGACACTGC CATTCAGCGC ATGCACGGTT 
CTCCTGGATG CGGAAGTGTA CAGCGTGCCC CTGGATGCCC AGTCTGATGA CAGTAAAACT 
TCTGTGAGGG ATCGCTTTAA TGCAAGACAG TTCATGTCAT GGTTACAAGA TGTGGATGAT 
AAATTCGACA AATTAAAGAC CTGTCTTTTA ATGAGGCAAC AGCATGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGTTAGA ATGGCAGCTC AAACTCCAGG AACTTGATCC TGCCACCTAT 
AAATCTATCA GCATCTATGA AATTCAGGAG TTTTACGTTC CGCTGGTTGA TGTTAACGAT 
GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697789.1
CDS	221..6304
Translation	MPKSGFTKPVQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIDRNDVGKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTPPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQIVPSKAPLPSALDEYEFKDDDEEEINKMIDDRHILRKELRKENESEVEKSSLFAKQEKTFYPKSFKSKKQKPSRVLYSSSESSDEEILQNKKCSAPCSVPETSNSDIQTKKEYGVSNEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRVTNLTVNTALDCSDKTRDEGNFRKSFSPKDDTSLHLFHISSGKSPKHSCGLSEKQSTPLKQEHSKTCLSPGSSEMSLQPDLVRYDNIESEFLPESSSVKSCKHKEKNKHHKDFHLEFGEKSNAKIKDDDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKSRDGTKELQRSVEFDREFWKENFFKSDETEDLFLSMEHESLTLEKKSKLEKNIKDDKSTKEKHISKERNFKEEREKIKKESEKSFREEKIKDLKDDRENVLTDKETEFSSLGVSATEETIGLHSVEKEIEIEKHMKESKEKSEKRFQTKDKDIERADRKNSEKEKKIKHEHKSEKDRLDPSECVDRIKEKDKLYSHHTEKCHREGEKLKNITTIKKNDDRDKSREKMDRKHDKEKPEKERHPVESKEKHLMEKKNKPSDNSDYTKSEKSKNKEKDREVDKKDKSRDSVNISNSKHFQEERKSSITDSNKAQHEKTLSLKEKTKDEPLRTPDGKEKDKKDIDRYKERDKHKDKILSSSLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRSCLELKVKDKEKTKHALAESKNKELTRSKSSELTDTYIKEKHCKDAVSNRSQSVDTKNTMSLGKSSFVSDNSLNRSPRSESEKPGLSSRSVSLISVASSEDSCLTTMTTPRPPVEYDSDFMLEGSDSQMSFSQSPFLPVAKSPALHDRELDSLTDLSERIKASYASRLLTSHLRASSVEDAKLVTNEGRSAAEARRCSMPSVICEHTKQFQTISEEGSQDGSAVPREICPSPKSEVSSNVPERELLSVSNIHSSFAASPTRSINSKYNSTDTNVIKGTAPLGMLMGSPVHLESSNQVGVIQSKSWEMESLNTGDFSCPNSAAPDQDSSVQGFCNPENKVLREQNIDFVSMHQTELPANTCAQDPASVLPSQQPCSSHSQSLSDAESIPRHTSLSYVTTQEPGILQQKNAIQMISSVLDTDTEPTKDMENTFVLTDVQKTDAFVPVYSESTVEEAAPNFEKASTLPVLPSEKDFNGSDGSSQPNPHYAFSKLMFKSSSGHEAEKSTSDTQDTSHEKENKLESLVLTHLNDKCDSDLCEMNVGVPKGNLSEPDNPKHCPESEKCLLSIEDEESQQSTLSSLENHPQPSAPPEMHKYGHLVKVELEESAEDDKPESQIPQRMTRNKGNTGASQSKQLLASCALLPDKDSESASPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYSVPLDAQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_002697743.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697743.4

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA AACAAAAAAG GAAGCTGGAA ATAAGAAGTC CACTCCAGTT 
AGCATTCTCT TTGGTTACCC ACTCTCAGAG CGAAAGCAGA TGGCACTTCT TATGCAGATG 
ACAGCACGGG ACAACAGTCC AGATTCCACA CCAAATCATC CATCGCAAAC ACCACCAGCC 
CAAAAGAAAA CTCCCAGTTC CTCATCTCGA CAAAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCAGCT ATTCGAGGAG ATGTAAAACA AGTCAAAGAA 
CTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ACGTGGGATA TTATGATGTT GCTAAGATAC TGATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCCGCTAG TAGTGGGCAC 
AGAGATATTG TGAAACTGCT GCTTCGTCAT GGTGGAAACC CTTTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTAGATGT GGCAGAAACA GAGGAGCTGG AGTTGTTGCT GAAAAGAGAG 
GTGCCTTTGT CTGATGATGA TGAGAGTTAC ACAGATTCTG AAGAGGCTCA GTCTGTAAAT 
CCTTCCAGCG TTGATGAAAA TATTGACTCT GAGACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TAGTTCCCAG TAAAGCACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGAAGAGGA AATAAATAAA ATGATTGATG ACAGGCATAT TCTTAGGAAA 
GAACTGCGAA AAGAGAATGA ATCTGAAGTA GAAAAGAGTA GTTTATTTGC AAAACAGGAA 
AAAACCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTTTTGTAT 
TCAAGTTCTG AAAGTTCAGA TGAAGAAATT CTTCAAAACA AAAAGTGTTC TGCTCCATGT 
TCTGTCCCTG AAACATCAAA TTCTGATATT CAAACCAAAA AGGAATATGG AGTTTCAAAT 
GAACACAAAC AGAAAGGCAA AGTTAAAAGA AAATTAAAGA ACCAGAACAA AAATAAAGAG 
AACCAAGAGC TGAAGCAAGA AAAAGAGGGG AAAGAAAATA CCAGAGTAAC AAACTTGACA 
GTTAATACTG CACTAGATTG TTCAGATAAG ACCAGAGATG AGGGGAATTT TAGGAAATCT 
TTTAGCCCGA AAGATGATAC TTCATTACAT TTATTTCATA TTTCCAGTGG TAAATCTCCC 
AAACATTCTT GTGGACTCAG TGAAAAGCAG TCAACACCAC TAAAACAAGA ACATTCTAAA 
ACCTGTTTAT CACCAGGAAG TTCTGAAATG TCATTACAGC CTGATCTTGT TCGGTATGAT 
AATATAGAAT CTGAATTCTT GCCAGAAAGT TCAAGTGTAA AATCTTGTAA GCATAAGGAA 
AAAAACAAAC ATCATAAAGA TTTCCACTTA GAATTTGGTG AAAAGTCAAA TGCCAAAATA 
AAAGATGATG ATCACAGTCC AACATTTGAA AATTCAGATT GCACACTGAA AAAAATGGAT 
AAAGAGGGTA AAACATTAAA AAAACATAAG TTAAAACATA AAGAAAGGGA AAAAGAAAAG 
CATAAAAAAG AAATTGAAGG TGAAAAGGAA AAATACAAAA GTAGGGATGG TACTAAAGAG 
CTGCAACGGA GTGTGGAATT TGATAGAGAG TTTTGGAAAG AGAATTTTTT TAAAAGTGAT 
GAAACCGAAG ATCTATTTTT AAGTATGGAA CATGAGTCCC TAACGTTAGA AAAAAAGTCA 
AAGTTGGAGA AAAACATAAA AGATGATAAA TCAACTAAGG AAAAGCATAT CTCCAAAGAG 
AGAAACTTTA AAGAAGAACG GGAAAAGATT AAAAAGGAAA GTGAGAAATC TTTTAGGGAG 
GAAAAAATAA AAGATTTAAA AGATGACAGA GAGAACGTAC TTACAGATAA AGAAACAGAA 
TTCAGCTCTT TAGGTGTAAG TGCCACTGAA GAGACTATAG GGTTACATTC AGTGGAAAAG 
GAAATAGAAA TTGAAAAGCA TATGAAAGAA AGTAAGGAAA AATCTGAGAA ACGATTTCAA 
ACTAAAGATA AGGACATTGA GAGGGCTGAC AGAAAAAATT CTGAAAAAGA GAAGAAGATA 
AAACATGAGC ATAAGTCAGA GAAAGACAGA TTAGATCCTA GTGAATGTGT CGACAGAATA 
AAAGAAAAGG ACAAGCTATA TTCACATCAC ACAGAAAAAT GCCATAGAGA AGGTGAGAAG 
CTAAAAAACA TTACTACCAT TAAAAAAAAC GATGACAGAG ATAAAAGTAG AGAAAAGATG 
GATAGGAAAC ATGACAAAGA AAAACCTGAG AAGGAGAGAC ATCCAGTAGA GAGCAAAGAG 
AAGCACTTGA TGGAAAAAAA AAACAAGCCA TCAGATAATA GTGACTATAC TAAATCAGAA 
AAAAGCAAAA ATAAAGAAAA AGATAGGGAG GTAGATAAAA AGGACAAATC TAGAGACAGT 
GTAAATATAA GTAATTCCAA ACACTTCCAG GAAGAGAGAA AGTCAAGTAT AACAGACAGT 
AATAAAGCAC AACATGAAAA AACTCTCTCT CTCAAAGAAA AAACAAAAGA TGAACCTTTG 
AGAACTCCTG ATGGAAAAGA AAAAGATAAA AAAGATATAG ATAGATATAA AGAACGAGAC 
AAACATAAGG ATAAAATTCT GAGTAGTTCA CTCAAACTAA AATCTGAAGC AGATAAGCCT 
AAACCGAAGT CATCACCAGC ATCAAAAGAT ACTCGGCCCA AAGAAAAGAG GCTTGTGAAT 
GACGACTTAA TGCAGACCAG CTTTGAACGG ATGCTGAGCC TTAAAGACTT GGAAATAGAG 
CAGTGGCACA GAAAACACAA GGAAAAGATT AAGCAAAAAG AAAAGGAACG GTTGAGAAAT 
CGCAGTTGTT TAGAACTTAA AGTAAAAGAT AAAGAAAAAA CAAAGCATGC ACTAGCTGAG 
TCCAAAAATA AAGAACTTAC TAGATCAAAG AGTTCTGAGT TGACTGATAC TTACATTAAG 
GAGAAACACT GTAAAGATGC TGTAAGTAAT AGATCCCAAT CTGTGGACAC CAAAAATACG 
ATGAGTTTAG GCAAGTCATC CTTTGTTTCA GATAATAGCT TGAACAGGTC TCCTAGATCA 
GAAAGTGAAA AGCCAGGTCT CAGCTCCAGG TCTGTCTCCT TGATTTCAGT GGCTAGCTCA 
GAAGATTCCT GCCTTACTAC AATGACAACC CCAAGGCCTC CAGTTGAGTA TGACTCAGAC 
TTTATGTTAG AGGGTTCGGA TTCCCAAATG TCCTTTTCCC AGTCACCGTT TTTGCCAGTT 
GCCAAATCTC CTGCCCTTCA TGACAGGGAG CTGGATAGCC TGACTGATCT ATCAGAGCGG 
ATTAAAGCAT CGTATGCTAG CAGGCTTCTG ACCTCCCATC TCCGGGCATC TTCTGTCGAA 
GATGCTAAAC TAGTTACAAA TGAAGGGAGA TCAGCTGCAG AAGCCCGAAG ATGTAGCATG 
CCCTCTGTCA TTTGTGAGCA TACAAAGCAA TTCCAAACAA TATCAGAAGA GGGCAGTCAA 
GATGGCTCAG CTGTGCCAAG AGAGATTTGT CCTTCTCCCA AATCTGAGGT ATCCTCCAAC 
GTGCCTGAAA GAGAACTTTT GAGCGTGTCC AACATACATT CCAGTTTTGC AGCCTCTCCA 
ACTAGATCTA TAAACAGCAA ATATAATTCT ACTGATACAA ATGTCATCAA GGGCACTGCT 
CCCTTGGGCA TGCTGATGGG CAGTCCTGTG CACTTAGAGT CCTCTAATCA GGTTGGTGTG 
ATCCAAAGTA AGTCATGGGA GATGGAGTCG TTAAACACCG GAGACTTTAG CTGCCCAAAT 
TCTGCTGCAC CTGATCAAGA TTCTTCTGTT CAGGGTTTTT GTAATCCTGA AAACAAAGTA 
TTGAGAGAAC AGAATATTGA TTTTGTGTCC ATGCACCAGA CTGAATTGCC GGCAAACACT 
TGTGCTCAGG ATCCAGCGTC CGTCCTTCCT TCCCAGCAAC CTTGCTCTTC GCATAGCCAA 
TCACTTTCAG ATGCTGAATC TATTCCCAGA CATACTTCTT TGTCCTATGT CACCACTCAA 
GAACCAGGTA TTCTACAGCA GAAAAATGCA ATTCAAATGA TCAGTTCTGT TTTGGATACT 
GATACTGAAC CTACAAAAGA TATGGAAAAT ACTTTTGTCC TAACAGACGT TCAGAAGACA 
GATGCCTTTG TCCCAGTGTA CTCTGAAAGC ACTGTTGAAG AAGCAGCACC AAATTTTGAG 
AAAGCTAGTA CTTTGCCAGT ATTGCCATCA GAAAAGGACT TTAATGGAAG TGACGGGTCT 
TCCCAGCCAA ATCCTCATTA TGCATTTAGC AAGCTAATGT TTAAGTCTTC CAGTGGCCAT 
GAGGCTGAAA AAAGCACTTC TGATACTCAG GATACTTCAC ATGAAAAGGA AAACAAACTG 
GAGAGTTTGG TTTTGACTCA CTTAAATGAT AAGTGTGATT CTGATTTATG TGAAATGAAT 
GTAGGGGTGC CAAAAGGAAA CCTGAGTGAA CCAGATAATC CAAAACATTG TCCTGAAAGT 
GAGAAGTGTT TGCTTTCCAT CGAGGATGAG GAGTCACAAC AAAGTACTTT ATCGAGTCTG 
GAGAACCATC CACAGCCATC GGCTCCACCA GAAATGCATA AATACGGACA CTTAGTTAAA 
GTAGAACTAG AGGAAAGTGC TGAAGATGAT AAACCTGAAA GCCAGATCCC TCAAAGGATG 
ACCAGAAACA AAGGAAACAC AGGAGCCAGT CAAAGCAAGC AGCTTCTGGC CAGCTGTGCA 
CTGTTGCCAG ACAAAGACAG TGAGTCTGCG TCCCCTCGAG GAAGAATAAG ATTAACTGAG 
GAGGACGACC CTCAGATTCA CCACCCGCGG AAAAGGAAAG TGTCCCGGGT GCCTCAGCCG 
GTGCAAGTGA GTCCTTCTCT GCTGCAAGCA AAAGAGAAAA CCCAGCAGTC CCTGGCCGCC 
ATTGTAGACT CGCTGAAGCT CGATGAGATT CAGCCATACA GCTCAGAGCG CGCAAATCCC 
TATTTTGAAT ACTTGCACAT AAGAAAAAAG ATTGAAGAAA AGCGCAAGTT GTTATGTAGT 
GTGATCCCTC AGGCACCTCA GTATTATGAT GAATACGTGA CGTTTAATGG GTCGTATCTC 
CTGGACGGAA ACCCCTTAAG CAAGATCTGC ATACCCACAA TTACACCCCC ACCTTCACTG 
TCAGATCCAC TTAAAGAGCT TTTTCGACAA CAGGAAGTTG TCAGGATGAA ACTACGTTTG 
CAGCACAGTA TTGAAAGGGA AAAACTCATT GTATCCAATG AACAGGAGGT TCTTCGGGTT 
CATTACAGAG CTGCAAGGAC ACTGGCAAAT CAGACACTGC CATTCAGCGC ATGCACGGTT 
CTCCTGGATG CGGAAGTGTA CAGCGTGCCC CTGGATGCCC AGTCTGATGA CAGTAAAACT 
TCTGTGAGGG ATCGCTTTAA TGCAAGACAG TTCATGTCAT GGTTACAAGA TGTGGATGAT 
AAATTCGACA AATTAAAGAC CTGTCTTTTA ATGAGGCAAC AGCATGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGTTAGA ATGGCAGCTC AAACTCCAGG AACTTGATCC TGCCACCTAT 
AAATCTATCA GCATCTATGA AATTCAGGAG TTTTACGTTC CGCTGGTTGA TGTTAACGAT 
GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_015469151.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324637.1 Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X6, mRNA.

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CloneID	OBa11573
Clone ID Related Accession (Same CDS sequence)	XM_002697743.4 , XM_015469151.1 , XM_024984610.1 , XM_002697743.5
Accession Version	XM_015469151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA AACAAAAAAG GAAGCTGGAA ATAAGAAGTC CACTCCAGTT 
AGCATTCTCT TTGGTTACCC ACTCTCAGAG CGAAAGCAGA TGGCACTTCT TATGCAGATG 
ACAGCACGGG ACAACAGTCC AGATTCCACA CCAAATCATC CATCGCAAAC ACCACCAGCC 
CAAAAGAAAA CTCCCAGTTC CTCATCTCGA CAAAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCAGCT ATTCGAGGAG ATGTAAAACA AGTCAAAGAA 
CTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ACGTGGGATA TTATGATGTT GCTAAGATAC TGATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCCGCTAG TAGTGGGCAC 
AGAGATATTG TGAAACTGCT GCTTCGTCAT GGTGGAAACC CTTTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTAGATGT GGCAGAAACA GAGGAGCTGG AGTTGTTGCT GAAAAGAGAG 
GTGCCTTTGT CTGATGATGA TGAGAGTTAC ACAGATTCTG AAGAGGCTCA GTCTGTAAAT 
CCTTCCAGCG TTGATGAAAA TATTGACTCT GAGACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TAGTTCCCAG TAAAGCACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGAAGAGGA AATAAATAAA ATGATTGATG ACAGGCATAT TCTTAGGAAA 
GAACTGCGAA AAGAGAATGA ATCTGAAGTA GAAAAGAGTA GTTTATTTGC AAAACAGGAA 
AAAACCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTTTTGTAT 
TCAAGTTCTG AAAGTTCAGA TGAAGAAATT CTTCAAAACA AAAAGTGTTC TGCTCCATGT 
TCTGTCCCTG AAACATCAAA TTCTGATATT CAAACCAAAA AGGAATATGG AGTTTCAAAT 
GAACACAAAC AGAAAGGCAA AGTTAAAAGA AAATTAAAGA ACCAGAACAA AAATAAAGAG 
AACCAAGAGC TGAAGCAAGA AAAAGAGGGG AAAGAAAATA CCAGAGTAAC AAACTTGACA 
GTTAATACTG CACTAGATTG TTCAGATAAG ACCAGAGATG AGGGGAATTT TAGGAAATCT 
TTTAGCCCGA AAGATGATAC TTCATTACAT TTATTTCATA TTTCCAGTGG TAAATCTCCC 
AAACATTCTT GTGGACTCAG TGAAAAGCAG TCAACACCAC TAAAACAAGA ACATTCTAAA 
ACCTGTTTAT CACCAGGAAG TTCTGAAATG TCATTACAGC CTGATCTTGT TCGGTATGAT 
AATATAGAAT CTGAATTCTT GCCAGAAAGT TCAAGTGTAA AATCTTGTAA GCATAAGGAA 
AAAAACAAAC ATCATAAAGA TTTCCACTTA GAATTTGGTG AAAAGTCAAA TGCCAAAATA 
AAAGATGATG ATCACAGTCC AACATTTGAA AATTCAGATT GCACACTGAA AAAAATGGAT 
AAAGAGGGTA AAACATTAAA AAAACATAAG TTAAAACATA AAGAAAGGGA AAAAGAAAAG 
CATAAAAAAG AAATTGAAGG TGAAAAGGAA AAATACAAAA GTAGGGATGG TACTAAAGAG 
CTGCAACGGA GTGTGGAATT TGATAGAGAG TTTTGGAAAG AGAATTTTTT TAAAAGTGAT 
GAAACCGAAG ATCTATTTTT AAGTATGGAA CATGAGTCCC TAACGTTAGA AAAAAAGTCA 
AAGTTGGAGA AAAACATAAA AGATGATAAA TCAACTAAGG AAAAGCATAT CTCCAAAGAG 
AGAAACTTTA AAGAAGAACG GGAAAAGATT AAAAAGGAAA GTGAGAAATC TTTTAGGGAG 
GAAAAAATAA AAGATTTAAA AGATGACAGA GAGAACGTAC TTACAGATAA AGAAACAGAA 
TTCAGCTCTT TAGGTGTAAG TGCCACTGAA GAGACTATAG GGTTACATTC AGTGGAAAAG 
GAAATAGAAA TTGAAAAGCA TATGAAAGAA AGTAAGGAAA AATCTGAGAA ACGATTTCAA 
ACTAAAGATA AGGACATTGA GAGGGCTGAC AGAAAAAATT CTGAAAAAGA GAAGAAGATA 
AAACATGAGC ATAAGTCAGA GAAAGACAGA TTAGATCCTA GTGAATGTGT CGACAGAATA 
AAAGAAAAGG ACAAGCTATA TTCACATCAC ACAGAAAAAT GCCATAGAGA AGGTGAGAAG 
CTAAAAAACA TTACTACCAT TAAAAAAAAC GATGACAGAG ATAAAAGTAG AGAAAAGATG 
GATAGGAAAC ATGACAAAGA AAAACCTGAG AAGGAGAGAC ATCCAGTAGA GAGCAAAGAG 
AAGCACTTGA TGGAAAAAAA AAACAAGCCA TCAGATAATA GTGACTATAC TAAATCAGAA 
AAAAGCAAAA ATAAAGAAAA AGATAGGGAG GTAGATAAAA AGGACAAATC TAGAGACAGT 
GTAAATATAA GTAATTCCAA ACACTTCCAG GAAGAGAGAA AGTCAAGTAT AACAGACAGT 
AATAAAGCAC AACATGAAAA AACTCTCTCT CTCAAAGAAA AAACAAAAGA TGAACCTTTG 
AGAACTCCTG ATGGAAAAGA AAAAGATAAA AAAGATATAG ATAGATATAA AGAACGAGAC 
AAACATAAGG ATAAAATTCT GAGTAGTTCA CTCAAACTAA AATCTGAAGC AGATAAGCCT 
AAACCGAAGT CATCACCAGC ATCAAAAGAT ACTCGGCCCA AAGAAAAGAG GCTTGTGAAT 
GACGACTTAA TGCAGACCAG CTTTGAACGG ATGCTGAGCC TTAAAGACTT GGAAATAGAG 
CAGTGGCACA GAAAACACAA GGAAAAGATT AAGCAAAAAG AAAAGGAACG GTTGAGAAAT 
CGCAGTTGTT TAGAACTTAA AGTAAAAGAT AAAGAAAAAA CAAAGCATGC ACTAGCTGAG 
TCCAAAAATA AAGAACTTAC TAGATCAAAG AGTTCTGAGT TGACTGATAC TTACATTAAG 
GAGAAACACT GTAAAGATGC TGTAAGTAAT AGATCCCAAT CTGTGGACAC CAAAAATACG 
ATGAGTTTAG GCAAGTCATC CTTTGTTTCA GATAATAGCT TGAACAGGTC TCCTAGATCA 
GAAAGTGAAA AGCCAGGTCT CAGCTCCAGG TCTGTCTCCT TGATTTCAGT GGCTAGCTCA 
GAAGATTCCT GCCTTACTAC AATGACAACC CCAAGGCCTC CAGTTGAGTA TGACTCAGAC 
TTTATGTTAG AGGGTTCGGA TTCCCAAATG TCCTTTTCCC AGTCACCGTT TTTGCCAGTT 
GCCAAATCTC CTGCCCTTCA TGACAGGGAG CTGGATAGCC TGACTGATCT ATCAGAGCGG 
ATTAAAGCAT CGTATGCTAG CAGGCTTCTG ACCTCCCATC TCCGGGCATC TTCTGTCGAA 
GATGCTAAAC TAGTTACAAA TGAAGGGAGA TCAGCTGCAG AAGCCCGAAG ATGTAGCATG 
CCCTCTGTCA TTTGTGAGCA TACAAAGCAA TTCCAAACAA TATCAGAAGA GGGCAGTCAA 
GATGGCTCAG CTGTGCCAAG AGAGATTTGT CCTTCTCCCA AATCTGAGGT ATCCTCCAAC 
GTGCCTGAAA GAGAACTTTT GAGCGTGTCC AACATACATT CCAGTTTTGC AGCCTCTCCA 
ACTAGATCTA TAAACAGCAA ATATAATTCT ACTGATACAA ATGTCATCAA GGGCACTGCT 
CCCTTGGGCA TGCTGATGGG CAGTCCTGTG CACTTAGAGT CCTCTAATCA GGTTGGTGTG 
ATCCAAAGTA AGTCATGGGA GATGGAGTCG TTAAACACCG GAGACTTTAG CTGCCCAAAT 
TCTGCTGCAC CTGATCAAGA TTCTTCTGTT CAGGGTTTTT GTAATCCTGA AAACAAAGTA 
TTGAGAGAAC AGAATATTGA TTTTGTGTCC ATGCACCAGA CTGAATTGCC GGCAAACACT 
TGTGCTCAGG ATCCAGCGTC CGTCCTTCCT TCCCAGCAAC CTTGCTCTTC GCATAGCCAA 
TCACTTTCAG ATGCTGAATC TATTCCCAGA CATACTTCTT TGTCCTATGT CACCACTCAA 
GAACCAGGTA TTCTACAGCA GAAAAATGCA ATTCAAATGA TCAGTTCTGT TTTGGATACT 
GATACTGAAC CTACAAAAGA TATGGAAAAT ACTTTTGTCC TAACAGACGT TCAGAAGACA 
GATGCCTTTG TCCCAGTGTA CTCTGAAAGC ACTGTTGAAG AAGCAGCACC AAATTTTGAG 
AAAGCTAGTA CTTTGCCAGT ATTGCCATCA GAAAAGGACT TTAATGGAAG TGACGGGTCT 
TCCCAGCCAA ATCCTCATTA TGCATTTAGC AAGCTAATGT TTAAGTCTTC CAGTGGCCAT 
GAGGCTGAAA AAAGCACTTC TGATACTCAG GATACTTCAC ATGAAAAGGA AAACAAACTG 
GAGAGTTTGG TTTTGACTCA CTTAAATGAT AAGTGTGATT CTGATTTATG TGAAATGAAT 
GTAGGGGTGC CAAAAGGAAA CCTGAGTGAA CCAGATAATC CAAAACATTG TCCTGAAAGT 
GAGAAGTGTT TGCTTTCCAT CGAGGATGAG GAGTCACAAC AAAGTACTTT ATCGAGTCTG 
GAGAACCATC CACAGCCATC GGCTCCACCA GAAATGCATA AATACGGACA CTTAGTTAAA 
GTAGAACTAG AGGAAAGTGC TGAAGATGAT AAACCTGAAA GCCAGATCCC TCAAAGGATG 
ACCAGAAACA AAGGAAACAC AGGAGCCAGT CAAAGCAAGC AGCTTCTGGC CAGCTGTGCA 
CTGTTGCCAG ACAAAGACAG TGAGTCTGCG TCCCCTCGAG GAAGAATAAG ATTAACTGAG 
GAGGACGACC CTCAGATTCA CCACCCGCGG AAAAGGAAAG TGTCCCGGGT GCCTCAGCCG 
GTGCAAGTGA GTCCTTCTCT GCTGCAAGCA AAAGAGAAAA CCCAGCAGTC CCTGGCCGCC 
ATTGTAGACT CGCTGAAGCT CGATGAGATT CAGCCATACA GCTCAGAGCG CGCAAATCCC 
TATTTTGAAT ACTTGCACAT AAGAAAAAAG ATTGAAGAAA AGCGCAAGTT GTTATGTAGT 
GTGATCCCTC AGGCACCTCA GTATTATGAT GAATACGTGA CGTTTAATGG GTCGTATCTC 
CTGGACGGAA ACCCCTTAAG CAAGATCTGC ATACCCACAA TTACACCCCC ACCTTCACTG 
TCAGATCCAC TTAAAGAGCT TTTTCGACAA CAGGAAGTTG TCAGGATGAA ACTACGTTTG 
CAGCACAGTA TTGAAAGGGA AAAACTCATT GTATCCAATG AACAGGAGGT TCTTCGGGTT 
CATTACAGAG CTGCAAGGAC ACTGGCAAAT CAGACACTGC CATTCAGCGC ATGCACGGTT 
CTCCTGGATG CGGAAGTGTA CAGCGTGCCC CTGGATGCCC AGTCTGATGA CAGTAAAACT 
TCTGTGAGGG ATCGCTTTAA TGCAAGACAG TTCATGTCAT GGTTACAAGA TGTGGATGAT 
AAATTCGACA AATTAAAGAC CTGTCTTTTA ATGAGGCAAC AGCATGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGTTAGA ATGGCAGCTC AAACTCCAGG AACTTGATCC TGCCACCTAT 
AAATCTATCA GCATCTATGA AATTCAGGAG TTTTACGTTC CGCTGGTTGA TGTTAACGAT 
GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324637.1
CDS	346..6429
Translation	MPKSGFTKPVQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIDRNDVGKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTPPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQIVPSKAPLPSALDEYEFKDDDEEEINKMIDDRHILRKELRKENESEVEKSSLFAKQEKTFYPKSFKSKKQKPSRVLYSSSESSDEEILQNKKCSAPCSVPETSNSDIQTKKEYGVSNEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRVTNLTVNTALDCSDKTRDEGNFRKSFSPKDDTSLHLFHISSGKSPKHSCGLSEKQSTPLKQEHSKTCLSPGSSEMSLQPDLVRYDNIESEFLPESSSVKSCKHKEKNKHHKDFHLEFGEKSNAKIKDDDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKSRDGTKELQRSVEFDREFWKENFFKSDETEDLFLSMEHESLTLEKKSKLEKNIKDDKSTKEKHISKERNFKEEREKIKKESEKSFREEKIKDLKDDRENVLTDKETEFSSLGVSATEETIGLHSVEKEIEIEKHMKESKEKSEKRFQTKDKDIERADRKNSEKEKKIKHEHKSEKDRLDPSECVDRIKEKDKLYSHHTEKCHREGEKLKNITTIKKNDDRDKSREKMDRKHDKEKPEKERHPVESKEKHLMEKKNKPSDNSDYTKSEKSKNKEKDREVDKKDKSRDSVNISNSKHFQEERKSSITDSNKAQHEKTLSLKEKTKDEPLRTPDGKEKDKKDIDRYKERDKHKDKILSSSLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRSCLELKVKDKEKTKHALAESKNKELTRSKSSELTDTYIKEKHCKDAVSNRSQSVDTKNTMSLGKSSFVSDNSLNRSPRSESEKPGLSSRSVSLISVASSEDSCLTTMTTPRPPVEYDSDFMLEGSDSQMSFSQSPFLPVAKSPALHDRELDSLTDLSERIKASYASRLLTSHLRASSVEDAKLVTNEGRSAAEARRCSMPSVICEHTKQFQTISEEGSQDGSAVPREICPSPKSEVSSNVPERELLSVSNIHSSFAASPTRSINSKYNSTDTNVIKGTAPLGMLMGSPVHLESSNQVGVIQSKSWEMESLNTGDFSCPNSAAPDQDSSVQGFCNPENKVLREQNIDFVSMHQTELPANTCAQDPASVLPSQQPCSSHSQSLSDAESIPRHTSLSYVTTQEPGILQQKNAIQMISSVLDTDTEPTKDMENTFVLTDVQKTDAFVPVYSESTVEEAAPNFEKASTLPVLPSEKDFNGSDGSSQPNPHYAFSKLMFKSSSGHEAEKSTSDTQDTSHEKENKLESLVLTHLNDKCDSDLCEMNVGVPKGNLSEPDNPKHCPESEKCLLSIEDEESQQSTLSSLENHPQPSAPPEMHKYGHLVKVELEESAEDDKPESQIPQRMTRNKGNTGASQSKQLLASCALLPDKDSESASPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYSVPLDAQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_015469151.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469151.1

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA AACAAAAAAG GAAGCTGGAA ATAAGAAGTC CACTCCAGTT 
AGCATTCTCT TTGGTTACCC ACTCTCAGAG CGAAAGCAGA TGGCACTTCT TATGCAGATG 
ACAGCACGGG ACAACAGTCC AGATTCCACA CCAAATCATC CATCGCAAAC ACCACCAGCC 
CAAAAGAAAA CTCCCAGTTC CTCATCTCGA CAAAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCAGCT ATTCGAGGAG ATGTAAAACA AGTCAAAGAA 
CTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ACGTGGGATA TTATGATGTT GCTAAGATAC TGATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCCGCTAG TAGTGGGCAC 
AGAGATATTG TGAAACTGCT GCTTCGTCAT GGTGGAAACC CTTTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTAGATGT GGCAGAAACA GAGGAGCTGG AGTTGTTGCT GAAAAGAGAG 
GTGCCTTTGT CTGATGATGA TGAGAGTTAC ACAGATTCTG AAGAGGCTCA GTCTGTAAAT 
CCTTCCAGCG TTGATGAAAA TATTGACTCT GAGACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TAGTTCCCAG TAAAGCACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGAAGAGGA AATAAATAAA ATGATTGATG ACAGGCATAT TCTTAGGAAA 
GAACTGCGAA AAGAGAATGA ATCTGAAGTA GAAAAGAGTA GTTTATTTGC AAAACAGGAA 
AAAACCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTTTTGTAT 
TCAAGTTCTG AAAGTTCAGA TGAAGAAATT CTTCAAAACA AAAAGTGTTC TGCTCCATGT 
TCTGTCCCTG AAACATCAAA TTCTGATATT CAAACCAAAA AGGAATATGG AGTTTCAAAT 
GAACACAAAC AGAAAGGCAA AGTTAAAAGA AAATTAAAGA ACCAGAACAA AAATAAAGAG 
AACCAAGAGC TGAAGCAAGA AAAAGAGGGG AAAGAAAATA CCAGAGTAAC AAACTTGACA 
GTTAATACTG CACTAGATTG TTCAGATAAG ACCAGAGATG AGGGGAATTT TAGGAAATCT 
TTTAGCCCGA AAGATGATAC TTCATTACAT TTATTTCATA TTTCCAGTGG TAAATCTCCC 
AAACATTCTT GTGGACTCAG TGAAAAGCAG TCAACACCAC TAAAACAAGA ACATTCTAAA 
ACCTGTTTAT CACCAGGAAG TTCTGAAATG TCATTACAGC CTGATCTTGT TCGGTATGAT 
AATATAGAAT CTGAATTCTT GCCAGAAAGT TCAAGTGTAA AATCTTGTAA GCATAAGGAA 
AAAAACAAAC ATCATAAAGA TTTCCACTTA GAATTTGGTG AAAAGTCAAA TGCCAAAATA 
AAAGATGATG ATCACAGTCC AACATTTGAA AATTCAGATT GCACACTGAA AAAAATGGAT 
AAAGAGGGTA AAACATTAAA AAAACATAAG TTAAAACATA AAGAAAGGGA AAAAGAAAAG 
CATAAAAAAG AAATTGAAGG TGAAAAGGAA AAATACAAAA GTAGGGATGG TACTAAAGAG 
CTGCAACGGA GTGTGGAATT TGATAGAGAG TTTTGGAAAG AGAATTTTTT TAAAAGTGAT 
GAAACCGAAG ATCTATTTTT AAGTATGGAA CATGAGTCCC TAACGTTAGA AAAAAAGTCA 
AAGTTGGAGA AAAACATAAA AGATGATAAA TCAACTAAGG AAAAGCATAT CTCCAAAGAG 
AGAAACTTTA AAGAAGAACG GGAAAAGATT AAAAAGGAAA GTGAGAAATC TTTTAGGGAG 
GAAAAAATAA AAGATTTAAA AGATGACAGA GAGAACGTAC TTACAGATAA AGAAACAGAA 
TTCAGCTCTT TAGGTGTAAG TGCCACTGAA GAGACTATAG GGTTACATTC AGTGGAAAAG 
GAAATAGAAA TTGAAAAGCA TATGAAAGAA AGTAAGGAAA AATCTGAGAA ACGATTTCAA 
ACTAAAGATA AGGACATTGA GAGGGCTGAC AGAAAAAATT CTGAAAAAGA GAAGAAGATA 
AAACATGAGC ATAAGTCAGA GAAAGACAGA TTAGATCCTA GTGAATGTGT CGACAGAATA 
AAAGAAAAGG ACAAGCTATA TTCACATCAC ACAGAAAAAT GCCATAGAGA AGGTGAGAAG 
CTAAAAAACA TTACTACCAT TAAAAAAAAC GATGACAGAG ATAAAAGTAG AGAAAAGATG 
GATAGGAAAC ATGACAAAGA AAAACCTGAG AAGGAGAGAC ATCCAGTAGA GAGCAAAGAG 
AAGCACTTGA TGGAAAAAAA AAACAAGCCA TCAGATAATA GTGACTATAC TAAATCAGAA 
AAAAGCAAAA ATAAAGAAAA AGATAGGGAG GTAGATAAAA AGGACAAATC TAGAGACAGT 
GTAAATATAA GTAATTCCAA ACACTTCCAG GAAGAGAGAA AGTCAAGTAT AACAGACAGT 
AATAAAGCAC AACATGAAAA AACTCTCTCT CTCAAAGAAA AAACAAAAGA TGAACCTTTG 
AGAACTCCTG ATGGAAAAGA AAAAGATAAA AAAGATATAG ATAGATATAA AGAACGAGAC 
AAACATAAGG ATAAAATTCT GAGTAGTTCA CTCAAACTAA AATCTGAAGC AGATAAGCCT 
AAACCGAAGT CATCACCAGC ATCAAAAGAT ACTCGGCCCA AAGAAAAGAG GCTTGTGAAT 
GACGACTTAA TGCAGACCAG CTTTGAACGG ATGCTGAGCC TTAAAGACTT GGAAATAGAG 
CAGTGGCACA GAAAACACAA GGAAAAGATT AAGCAAAAAG AAAAGGAACG GTTGAGAAAT 
CGCAGTTGTT TAGAACTTAA AGTAAAAGAT AAAGAAAAAA CAAAGCATGC ACTAGCTGAG 
TCCAAAAATA AAGAACTTAC TAGATCAAAG AGTTCTGAGT TGACTGATAC TTACATTAAG 
GAGAAACACT GTAAAGATGC TGTAAGTAAT AGATCCCAAT CTGTGGACAC CAAAAATACG 
ATGAGTTTAG GCAAGTCATC CTTTGTTTCA GATAATAGCT TGAACAGGTC TCCTAGATCA 
GAAAGTGAAA AGCCAGGTCT CAGCTCCAGG TCTGTCTCCT TGATTTCAGT GGCTAGCTCA 
GAAGATTCCT GCCTTACTAC AATGACAACC CCAAGGCCTC CAGTTGAGTA TGACTCAGAC 
TTTATGTTAG AGGGTTCGGA TTCCCAAATG TCCTTTTCCC AGTCACCGTT TTTGCCAGTT 
GCCAAATCTC CTGCCCTTCA TGACAGGGAG CTGGATAGCC TGACTGATCT ATCAGAGCGG 
ATTAAAGCAT CGTATGCTAG CAGGCTTCTG ACCTCCCATC TCCGGGCATC TTCTGTCGAA 
GATGCTAAAC TAGTTACAAA TGAAGGGAGA TCAGCTGCAG AAGCCCGAAG ATGTAGCATG 
CCCTCTGTCA TTTGTGAGCA TACAAAGCAA TTCCAAACAA TATCAGAAGA GGGCAGTCAA 
GATGGCTCAG CTGTGCCAAG AGAGATTTGT CCTTCTCCCA AATCTGAGGT ATCCTCCAAC 
GTGCCTGAAA GAGAACTTTT GAGCGTGTCC AACATACATT CCAGTTTTGC AGCCTCTCCA 
ACTAGATCTA TAAACAGCAA ATATAATTCT ACTGATACAA ATGTCATCAA GGGCACTGCT 
CCCTTGGGCA TGCTGATGGG CAGTCCTGTG CACTTAGAGT CCTCTAATCA GGTTGGTGTG 
ATCCAAAGTA AGTCATGGGA GATGGAGTCG TTAAACACCG GAGACTTTAG CTGCCCAAAT 
TCTGCTGCAC CTGATCAAGA TTCTTCTGTT CAGGGTTTTT GTAATCCTGA AAACAAAGTA 
TTGAGAGAAC AGAATATTGA TTTTGTGTCC ATGCACCAGA CTGAATTGCC GGCAAACACT 
TGTGCTCAGG ATCCAGCGTC CGTCCTTCCT TCCCAGCAAC CTTGCTCTTC GCATAGCCAA 
TCACTTTCAG ATGCTGAATC TATTCCCAGA CATACTTCTT TGTCCTATGT CACCACTCAA 
GAACCAGGTA TTCTACAGCA GAAAAATGCA ATTCAAATGA TCAGTTCTGT TTTGGATACT 
GATACTGAAC CTACAAAAGA TATGGAAAAT ACTTTTGTCC TAACAGACGT TCAGAAGACA 
GATGCCTTTG TCCCAGTGTA CTCTGAAAGC ACTGTTGAAG AAGCAGCACC AAATTTTGAG 
AAAGCTAGTA CTTTGCCAGT ATTGCCATCA GAAAAGGACT TTAATGGAAG TGACGGGTCT 
TCCCAGCCAA ATCCTCATTA TGCATTTAGC AAGCTAATGT TTAAGTCTTC CAGTGGCCAT 
GAGGCTGAAA AAAGCACTTC TGATACTCAG GATACTTCAC ATGAAAAGGA AAACAAACTG 
GAGAGTTTGG TTTTGACTCA CTTAAATGAT AAGTGTGATT CTGATTTATG TGAAATGAAT 
GTAGGGGTGC CAAAAGGAAA CCTGAGTGAA CCAGATAATC CAAAACATTG TCCTGAAAGT 
GAGAAGTGTT TGCTTTCCAT CGAGGATGAG GAGTCACAAC AAAGTACTTT ATCGAGTCTG 
GAGAACCATC CACAGCCATC GGCTCCACCA GAAATGCATA AATACGGACA CTTAGTTAAA 
GTAGAACTAG AGGAAAGTGC TGAAGATGAT AAACCTGAAA GCCAGATCCC TCAAAGGATG 
ACCAGAAACA AAGGAAACAC AGGAGCCAGT CAAAGCAAGC AGCTTCTGGC CAGCTGTGCA 
CTGTTGCCAG ACAAAGACAG TGAGTCTGCG TCCCCTCGAG GAAGAATAAG ATTAACTGAG 
GAGGACGACC CTCAGATTCA CCACCCGCGG AAAAGGAAAG TGTCCCGGGT GCCTCAGCCG 
GTGCAAGTGA GTCCTTCTCT GCTGCAAGCA AAAGAGAAAA CCCAGCAGTC CCTGGCCGCC 
ATTGTAGACT CGCTGAAGCT CGATGAGATT CAGCCATACA GCTCAGAGCG CGCAAATCCC 
TATTTTGAAT ACTTGCACAT AAGAAAAAAG ATTGAAGAAA AGCGCAAGTT GTTATGTAGT 
GTGATCCCTC AGGCACCTCA GTATTATGAT GAATACGTGA CGTTTAATGG GTCGTATCTC 
CTGGACGGAA ACCCCTTAAG CAAGATCTGC ATACCCACAA TTACACCCCC ACCTTCACTG 
TCAGATCCAC TTAAAGAGCT TTTTCGACAA CAGGAAGTTG TCAGGATGAA ACTACGTTTG 
CAGCACAGTA TTGAAAGGGA AAAACTCATT GTATCCAATG AACAGGAGGT TCTTCGGGTT 
CATTACAGAG CTGCAAGGAC ACTGGCAAAT CAGACACTGC CATTCAGCGC ATGCACGGTT 
CTCCTGGATG CGGAAGTGTA CAGCGTGCCC CTGGATGCCC AGTCTGATGA CAGTAAAACT 
TCTGTGAGGG ATCGCTTTAA TGCAAGACAG TTCATGTCAT GGTTACAAGA TGTGGATGAT 
AAATTCGACA AATTAAAGAC CTGTCTTTTA ATGAGGCAAC AGCATGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGTTAGA ATGGCAGCTC AAACTCCAGG AACTTGATCC TGCCACCTAT 
AAATCTATCA GCATCTATGA AATTCAGGAG TTTTACGTTC CGCTGGTTGA TGTTAACGAT 
GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_024984610.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840378.1 Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X3, mRNA.

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CloneID	OBa11573
Clone ID Related Accession (Same CDS sequence)	XM_002697743.4 , XM_015469151.1 , XM_024984610.1 , XM_002697743.5
Accession Version	XM_024984610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA AACAAAAAAG GAAGCTGGAA ATAAGAAGTC CACTCCAGTT 
AGCATTCTCT TTGGTTACCC ACTCTCAGAG CGAAAGCAGA TGGCACTTCT TATGCAGATG 
ACAGCACGGG ACAACAGTCC AGATTCCACA CCAAATCATC CATCGCAAAC ACCACCAGCC 
CAAAAGAAAA CTCCCAGTTC CTCATCTCGA CAAAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCAGCT ATTCGAGGAG ATGTAAAACA AGTCAAAGAA 
CTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ACGTGGGATA TTATGATGTT GCTAAGATAC TGATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCCGCTAG TAGTGGGCAC 
AGAGATATTG TGAAACTGCT GCTTCGTCAT GGTGGAAACC CTTTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTAGATGT GGCAGAAACA GAGGAGCTGG AGTTGTTGCT GAAAAGAGAG 
GTGCCTTTGT CTGATGATGA TGAGAGTTAC ACAGATTCTG AAGAGGCTCA GTCTGTAAAT 
CCTTCCAGCG TTGATGAAAA TATTGACTCT GAGACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TAGTTCCCAG TAAAGCACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGAAGAGGA AATAAATAAA ATGATTGATG ACAGGCATAT TCTTAGGAAA 
GAACTGCGAA AAGAGAATGA ATCTGAAGTA GAAAAGAGTA GTTTATTTGC AAAACAGGAA 
AAAACCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTTTTGTAT 
TCAAGTTCTG AAAGTTCAGA TGAAGAAATT CTTCAAAACA AAAAGTGTTC TGCTCCATGT 
TCTGTCCCTG AAACATCAAA TTCTGATATT CAAACCAAAA AGGAATATGG AGTTTCAAAT 
GAACACAAAC AGAAAGGCAA AGTTAAAAGA AAATTAAAGA ACCAGAACAA AAATAAAGAG 
AACCAAGAGC TGAAGCAAGA AAAAGAGGGG AAAGAAAATA CCAGAGTAAC AAACTTGACA 
GTTAATACTG CACTAGATTG TTCAGATAAG ACCAGAGATG AGGGGAATTT TAGGAAATCT 
TTTAGCCCGA AAGATGATAC TTCATTACAT TTATTTCATA TTTCCAGTGG TAAATCTCCC 
AAACATTCTT GTGGACTCAG TGAAAAGCAG TCAACACCAC TAAAACAAGA ACATTCTAAA 
ACCTGTTTAT CACCAGGAAG TTCTGAAATG TCATTACAGC CTGATCTTGT TCGGTATGAT 
AATATAGAAT CTGAATTCTT GCCAGAAAGT TCAAGTGTAA AATCTTGTAA GCATAAGGAA 
AAAAACAAAC ATCATAAAGA TTTCCACTTA GAATTTGGTG AAAAGTCAAA TGCCAAAATA 
AAAGATGATG ATCACAGTCC AACATTTGAA AATTCAGATT GCACACTGAA AAAAATGGAT 
AAAGAGGGTA AAACATTAAA AAAACATAAG TTAAAACATA AAGAAAGGGA AAAAGAAAAG 
CATAAAAAAG AAATTGAAGG TGAAAAGGAA AAATACAAAA GTAGGGATGG TACTAAAGAG 
CTGCAACGGA GTGTGGAATT TGATAGAGAG TTTTGGAAAG AGAATTTTTT TAAAAGTGAT 
GAAACCGAAG ATCTATTTTT AAGTATGGAA CATGAGTCCC TAACGTTAGA AAAAAAGTCA 
AAGTTGGAGA AAAACATAAA AGATGATAAA TCAACTAAGG AAAAGCATAT CTCCAAAGAG 
AGAAACTTTA AAGAAGAACG GGAAAAGATT AAAAAGGAAA GTGAGAAATC TTTTAGGGAG 
GAAAAAATAA AAGATTTAAA AGATGACAGA GAGAACGTAC TTACAGATAA AGAAACAGAA 
TTCAGCTCTT TAGGTGTAAG TGCCACTGAA GAGACTATAG GGTTACATTC AGTGGAAAAG 
GAAATAGAAA TTGAAAAGCA TATGAAAGAA AGTAAGGAAA AATCTGAGAA ACGATTTCAA 
ACTAAAGATA AGGACATTGA GAGGGCTGAC AGAAAAAATT CTGAAAAAGA GAAGAAGATA 
AAACATGAGC ATAAGTCAGA GAAAGACAGA TTAGATCCTA GTGAATGTGT CGACAGAATA 
AAAGAAAAGG ACAAGCTATA TTCACATCAC ACAGAAAAAT GCCATAGAGA AGGTGAGAAG 
CTAAAAAACA TTACTACCAT TAAAAAAAAC GATGACAGAG ATAAAAGTAG AGAAAAGATG 
GATAGGAAAC ATGACAAAGA AAAACCTGAG AAGGAGAGAC ATCCAGTAGA GAGCAAAGAG 
AAGCACTTGA TGGAAAAAAA AAACAAGCCA TCAGATAATA GTGACTATAC TAAATCAGAA 
AAAAGCAAAA ATAAAGAAAA AGATAGGGAG GTAGATAAAA AGGACAAATC TAGAGACAGT 
GTAAATATAA GTAATTCCAA ACACTTCCAG GAAGAGAGAA AGTCAAGTAT AACAGACAGT 
AATAAAGCAC AACATGAAAA AACTCTCTCT CTCAAAGAAA AAACAAAAGA TGAACCTTTG 
AGAACTCCTG ATGGAAAAGA AAAAGATAAA AAAGATATAG ATAGATATAA AGAACGAGAC 
AAACATAAGG ATAAAATTCT GAGTAGTTCA CTCAAACTAA AATCTGAAGC AGATAAGCCT 
AAACCGAAGT CATCACCAGC ATCAAAAGAT ACTCGGCCCA AAGAAAAGAG GCTTGTGAAT 
GACGACTTAA TGCAGACCAG CTTTGAACGG ATGCTGAGCC TTAAAGACTT GGAAATAGAG 
CAGTGGCACA GAAAACACAA GGAAAAGATT AAGCAAAAAG AAAAGGAACG GTTGAGAAAT 
CGCAGTTGTT TAGAACTTAA AGTAAAAGAT AAAGAAAAAA CAAAGCATGC ACTAGCTGAG 
TCCAAAAATA AAGAACTTAC TAGATCAAAG AGTTCTGAGT TGACTGATAC TTACATTAAG 
GAGAAACACT GTAAAGATGC TGTAAGTAAT AGATCCCAAT CTGTGGACAC CAAAAATACG 
ATGAGTTTAG GCAAGTCATC CTTTGTTTCA GATAATAGCT TGAACAGGTC TCCTAGATCA 
GAAAGTGAAA AGCCAGGTCT CAGCTCCAGG TCTGTCTCCT TGATTTCAGT GGCTAGCTCA 
GAAGATTCCT GCCTTACTAC AATGACAACC CCAAGGCCTC CAGTTGAGTA TGACTCAGAC 
TTTATGTTAG AGGGTTCGGA TTCCCAAATG TCCTTTTCCC AGTCACCGTT TTTGCCAGTT 
GCCAAATCTC CTGCCCTTCA TGACAGGGAG CTGGATAGCC TGACTGATCT ATCAGAGCGG 
ATTAAAGCAT CGTATGCTAG CAGGCTTCTG ACCTCCCATC TCCGGGCATC TTCTGTCGAA 
GATGCTAAAC TAGTTACAAA TGAAGGGAGA TCAGCTGCAG AAGCCCGAAG ATGTAGCATG 
CCCTCTGTCA TTTGTGAGCA TACAAAGCAA TTCCAAACAA TATCAGAAGA GGGCAGTCAA 
GATGGCTCAG CTGTGCCAAG AGAGATTTGT CCTTCTCCCA AATCTGAGGT ATCCTCCAAC 
GTGCCTGAAA GAGAACTTTT GAGCGTGTCC AACATACATT CCAGTTTTGC AGCCTCTCCA 
ACTAGATCTA TAAACAGCAA ATATAATTCT ACTGATACAA ATGTCATCAA GGGCACTGCT 
CCCTTGGGCA TGCTGATGGG CAGTCCTGTG CACTTAGAGT CCTCTAATCA GGTTGGTGTG 
ATCCAAAGTA AGTCATGGGA GATGGAGTCG TTAAACACCG GAGACTTTAG CTGCCCAAAT 
TCTGCTGCAC CTGATCAAGA TTCTTCTGTT CAGGGTTTTT GTAATCCTGA AAACAAAGTA 
TTGAGAGAAC AGAATATTGA TTTTGTGTCC ATGCACCAGA CTGAATTGCC GGCAAACACT 
TGTGCTCAGG ATCCAGCGTC CGTCCTTCCT TCCCAGCAAC CTTGCTCTTC GCATAGCCAA 
TCACTTTCAG ATGCTGAATC TATTCCCAGA CATACTTCTT TGTCCTATGT CACCACTCAA 
GAACCAGGTA TTCTACAGCA GAAAAATGCA ATTCAAATGA TCAGTTCTGT TTTGGATACT 
GATACTGAAC CTACAAAAGA TATGGAAAAT ACTTTTGTCC TAACAGACGT TCAGAAGACA 
GATGCCTTTG TCCCAGTGTA CTCTGAAAGC ACTGTTGAAG AAGCAGCACC AAATTTTGAG 
AAAGCTAGTA CTTTGCCAGT ATTGCCATCA GAAAAGGACT TTAATGGAAG TGACGGGTCT 
TCCCAGCCAA ATCCTCATTA TGCATTTAGC AAGCTAATGT TTAAGTCTTC CAGTGGCCAT 
GAGGCTGAAA AAAGCACTTC TGATACTCAG GATACTTCAC ATGAAAAGGA AAACAAACTG 
GAGAGTTTGG TTTTGACTCA CTTAAATGAT AAGTGTGATT CTGATTTATG TGAAATGAAT 
GTAGGGGTGC CAAAAGGAAA CCTGAGTGAA CCAGATAATC CAAAACATTG TCCTGAAAGT 
GAGAAGTGTT TGCTTTCCAT CGAGGATGAG GAGTCACAAC AAAGTACTTT ATCGAGTCTG 
GAGAACCATC CACAGCCATC GGCTCCACCA GAAATGCATA AATACGGACA CTTAGTTAAA 
GTAGAACTAG AGGAAAGTGC TGAAGATGAT AAACCTGAAA GCCAGATCCC TCAAAGGATG 
ACCAGAAACA AAGGAAACAC AGGAGCCAGT CAAAGCAAGC AGCTTCTGGC CAGCTGTGCA 
CTGTTGCCAG ACAAAGACAG TGAGTCTGCG TCCCCTCGAG GAAGAATAAG ATTAACTGAG 
GAGGACGACC CTCAGATTCA CCACCCGCGG AAAAGGAAAG TGTCCCGGGT GCCTCAGCCG 
GTGCAAGTGA GTCCTTCTCT GCTGCAAGCA AAAGAGAAAA CCCAGCAGTC CCTGGCCGCC 
ATTGTAGACT CGCTGAAGCT CGATGAGATT CAGCCATACA GCTCAGAGCG CGCAAATCCC 
TATTTTGAAT ACTTGCACAT AAGAAAAAAG ATTGAAGAAA AGCGCAAGTT GTTATGTAGT 
GTGATCCCTC AGGCACCTCA GTATTATGAT GAATACGTGA CGTTTAATGG GTCGTATCTC 
CTGGACGGAA ACCCCTTAAG CAAGATCTGC ATACCCACAA TTACACCCCC ACCTTCACTG 
TCAGATCCAC TTAAAGAGCT TTTTCGACAA CAGGAAGTTG TCAGGATGAA ACTACGTTTG 
CAGCACAGTA TTGAAAGGGA AAAACTCATT GTATCCAATG AACAGGAGGT TCTTCGGGTT 
CATTACAGAG CTGCAAGGAC ACTGGCAAAT CAGACACTGC CATTCAGCGC ATGCACGGTT 
CTCCTGGATG CGGAAGTGTA CAGCGTGCCC CTGGATGCCC AGTCTGATGA CAGTAAAACT 
TCTGTGAGGG ATCGCTTTAA TGCAAGACAG TTCATGTCAT GGTTACAAGA TGTGGATGAT 
AAATTCGACA AATTAAAGAC CTGTCTTTTA ATGAGGCAAC AGCATGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGTTAGA ATGGCAGCTC AAACTCCAGG AACTTGATCC TGCCACCTAT 
AAATCTATCA GCATCTATGA AATTCAGGAG TTTTACGTTC CGCTGGTTGA TGTTAACGAT 
GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840378.1
CDS	363..6446
Translation	MPKSGFTKPVQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIDRNDVGKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTPPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQIVPSKAPLPSALDEYEFKDDDEEEINKMIDDRHILRKELRKENESEVEKSSLFAKQEKTFYPKSFKSKKQKPSRVLYSSSESSDEEILQNKKCSAPCSVPETSNSDIQTKKEYGVSNEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRVTNLTVNTALDCSDKTRDEGNFRKSFSPKDDTSLHLFHISSGKSPKHSCGLSEKQSTPLKQEHSKTCLSPGSSEMSLQPDLVRYDNIESEFLPESSSVKSCKHKEKNKHHKDFHLEFGEKSNAKIKDDDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKSRDGTKELQRSVEFDREFWKENFFKSDETEDLFLSMEHESLTLEKKSKLEKNIKDDKSTKEKHISKERNFKEEREKIKKESEKSFREEKIKDLKDDRENVLTDKETEFSSLGVSATEETIGLHSVEKEIEIEKHMKESKEKSEKRFQTKDKDIERADRKNSEKEKKIKHEHKSEKDRLDPSECVDRIKEKDKLYSHHTEKCHREGEKLKNITTIKKNDDRDKSREKMDRKHDKEKPEKERHPVESKEKHLMEKKNKPSDNSDYTKSEKSKNKEKDREVDKKDKSRDSVNISNSKHFQEERKSSITDSNKAQHEKTLSLKEKTKDEPLRTPDGKEKDKKDIDRYKERDKHKDKILSSSLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRSCLELKVKDKEKTKHALAESKNKELTRSKSSELTDTYIKEKHCKDAVSNRSQSVDTKNTMSLGKSSFVSDNSLNRSPRSESEKPGLSSRSVSLISVASSEDSCLTTMTTPRPPVEYDSDFMLEGSDSQMSFSQSPFLPVAKSPALHDRELDSLTDLSERIKASYASRLLTSHLRASSVEDAKLVTNEGRSAAEARRCSMPSVICEHTKQFQTISEEGSQDGSAVPREICPSPKSEVSSNVPERELLSVSNIHSSFAASPTRSINSKYNSTDTNVIKGTAPLGMLMGSPVHLESSNQVGVIQSKSWEMESLNTGDFSCPNSAAPDQDSSVQGFCNPENKVLREQNIDFVSMHQTELPANTCAQDPASVLPSQQPCSSHSQSLSDAESIPRHTSLSYVTTQEPGILQQKNAIQMISSVLDTDTEPTKDMENTFVLTDVQKTDAFVPVYSESTVEEAAPNFEKASTLPVLPSEKDFNGSDGSSQPNPHYAFSKLMFKSSSGHEAEKSTSDTQDTSHEKENKLESLVLTHLNDKCDSDLCEMNVGVPKGNLSEPDNPKHCPESEKCLLSIEDEESQQSTLSSLENHPQPSAPPEMHKYGHLVKVELEESAEDDKPESQIPQRMTRNKGNTGASQSKQLLASCALLPDKDSESASPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYSVPLDAQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_024984610.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984610.1

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA AACAAAAAAG GAAGCTGGAA ATAAGAAGTC CACTCCAGTT 
AGCATTCTCT TTGGTTACCC ACTCTCAGAG CGAAAGCAGA TGGCACTTCT TATGCAGATG 
ACAGCACGGG ACAACAGTCC AGATTCCACA CCAAATCATC CATCGCAAAC ACCACCAGCC 
CAAAAGAAAA CTCCCAGTTC CTCATCTCGA CAAAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCAGCT ATTCGAGGAG ATGTAAAACA AGTCAAAGAA 
CTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ACGTGGGATA TTATGATGTT GCTAAGATAC TGATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCCGCTAG TAGTGGGCAC 
AGAGATATTG TGAAACTGCT GCTTCGTCAT GGTGGAAACC CTTTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTAGATGT GGCAGAAACA GAGGAGCTGG AGTTGTTGCT GAAAAGAGAG 
GTGCCTTTGT CTGATGATGA TGAGAGTTAC ACAGATTCTG AAGAGGCTCA GTCTGTAAAT 
CCTTCCAGCG TTGATGAAAA TATTGACTCT GAGACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TAGTTCCCAG TAAAGCACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGAAGAGGA AATAAATAAA ATGATTGATG ACAGGCATAT TCTTAGGAAA 
GAACTGCGAA AAGAGAATGA ATCTGAAGTA GAAAAGAGTA GTTTATTTGC AAAACAGGAA 
AAAACCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTTTTGTAT 
TCAAGTTCTG AAAGTTCAGA TGAAGAAATT CTTCAAAACA AAAAGTGTTC TGCTCCATGT 
TCTGTCCCTG AAACATCAAA TTCTGATATT CAAACCAAAA AGGAATATGG AGTTTCAAAT 
GAACACAAAC AGAAAGGCAA AGTTAAAAGA AAATTAAAGA ACCAGAACAA AAATAAAGAG 
AACCAAGAGC TGAAGCAAGA AAAAGAGGGG AAAGAAAATA CCAGAGTAAC AAACTTGACA 
GTTAATACTG CACTAGATTG TTCAGATAAG ACCAGAGATG AGGGGAATTT TAGGAAATCT 
TTTAGCCCGA AAGATGATAC TTCATTACAT TTATTTCATA TTTCCAGTGG TAAATCTCCC 
AAACATTCTT GTGGACTCAG TGAAAAGCAG TCAACACCAC TAAAACAAGA ACATTCTAAA 
ACCTGTTTAT CACCAGGAAG TTCTGAAATG TCATTACAGC CTGATCTTGT TCGGTATGAT 
AATATAGAAT CTGAATTCTT GCCAGAAAGT TCAAGTGTAA AATCTTGTAA GCATAAGGAA 
AAAAACAAAC ATCATAAAGA TTTCCACTTA GAATTTGGTG AAAAGTCAAA TGCCAAAATA 
AAAGATGATG ATCACAGTCC AACATTTGAA AATTCAGATT GCACACTGAA AAAAATGGAT 
AAAGAGGGTA AAACATTAAA AAAACATAAG TTAAAACATA AAGAAAGGGA AAAAGAAAAG 
CATAAAAAAG AAATTGAAGG TGAAAAGGAA AAATACAAAA GTAGGGATGG TACTAAAGAG 
CTGCAACGGA GTGTGGAATT TGATAGAGAG TTTTGGAAAG AGAATTTTTT TAAAAGTGAT 
GAAACCGAAG ATCTATTTTT AAGTATGGAA CATGAGTCCC TAACGTTAGA AAAAAAGTCA 
AAGTTGGAGA AAAACATAAA AGATGATAAA TCAACTAAGG AAAAGCATAT CTCCAAAGAG 
AGAAACTTTA AAGAAGAACG GGAAAAGATT AAAAAGGAAA GTGAGAAATC TTTTAGGGAG 
GAAAAAATAA AAGATTTAAA AGATGACAGA GAGAACGTAC TTACAGATAA AGAAACAGAA 
TTCAGCTCTT TAGGTGTAAG TGCCACTGAA GAGACTATAG GGTTACATTC AGTGGAAAAG 
GAAATAGAAA TTGAAAAGCA TATGAAAGAA AGTAAGGAAA AATCTGAGAA ACGATTTCAA 
ACTAAAGATA AGGACATTGA GAGGGCTGAC AGAAAAAATT CTGAAAAAGA GAAGAAGATA 
AAACATGAGC ATAAGTCAGA GAAAGACAGA TTAGATCCTA GTGAATGTGT CGACAGAATA 
AAAGAAAAGG ACAAGCTATA TTCACATCAC ACAGAAAAAT GCCATAGAGA AGGTGAGAAG 
CTAAAAAACA TTACTACCAT TAAAAAAAAC GATGACAGAG ATAAAAGTAG AGAAAAGATG 
GATAGGAAAC ATGACAAAGA AAAACCTGAG AAGGAGAGAC ATCCAGTAGA GAGCAAAGAG 
AAGCACTTGA TGGAAAAAAA AAACAAGCCA TCAGATAATA GTGACTATAC TAAATCAGAA 
AAAAGCAAAA ATAAAGAAAA AGATAGGGAG GTAGATAAAA AGGACAAATC TAGAGACAGT 
GTAAATATAA GTAATTCCAA ACACTTCCAG GAAGAGAGAA AGTCAAGTAT AACAGACAGT 
AATAAAGCAC AACATGAAAA AACTCTCTCT CTCAAAGAAA AAACAAAAGA TGAACCTTTG 
AGAACTCCTG ATGGAAAAGA AAAAGATAAA AAAGATATAG ATAGATATAA AGAACGAGAC 
AAACATAAGG ATAAAATTCT GAGTAGTTCA CTCAAACTAA AATCTGAAGC AGATAAGCCT 
AAACCGAAGT CATCACCAGC ATCAAAAGAT ACTCGGCCCA AAGAAAAGAG GCTTGTGAAT 
GACGACTTAA TGCAGACCAG CTTTGAACGG ATGCTGAGCC TTAAAGACTT GGAAATAGAG 
CAGTGGCACA GAAAACACAA GGAAAAGATT AAGCAAAAAG AAAAGGAACG GTTGAGAAAT 
CGCAGTTGTT TAGAACTTAA AGTAAAAGAT AAAGAAAAAA CAAAGCATGC ACTAGCTGAG 
TCCAAAAATA AAGAACTTAC TAGATCAAAG AGTTCTGAGT TGACTGATAC TTACATTAAG 
GAGAAACACT GTAAAGATGC TGTAAGTAAT AGATCCCAAT CTGTGGACAC CAAAAATACG 
ATGAGTTTAG GCAAGTCATC CTTTGTTTCA GATAATAGCT TGAACAGGTC TCCTAGATCA 
GAAAGTGAAA AGCCAGGTCT CAGCTCCAGG TCTGTCTCCT TGATTTCAGT GGCTAGCTCA 
GAAGATTCCT GCCTTACTAC AATGACAACC CCAAGGCCTC CAGTTGAGTA TGACTCAGAC 
TTTATGTTAG AGGGTTCGGA TTCCCAAATG TCCTTTTCCC AGTCACCGTT TTTGCCAGTT 
GCCAAATCTC CTGCCCTTCA TGACAGGGAG CTGGATAGCC TGACTGATCT ATCAGAGCGG 
ATTAAAGCAT CGTATGCTAG CAGGCTTCTG ACCTCCCATC TCCGGGCATC TTCTGTCGAA 
GATGCTAAAC TAGTTACAAA TGAAGGGAGA TCAGCTGCAG AAGCCCGAAG ATGTAGCATG 
CCCTCTGTCA TTTGTGAGCA TACAAAGCAA TTCCAAACAA TATCAGAAGA GGGCAGTCAA 
GATGGCTCAG CTGTGCCAAG AGAGATTTGT CCTTCTCCCA AATCTGAGGT ATCCTCCAAC 
GTGCCTGAAA GAGAACTTTT GAGCGTGTCC AACATACATT CCAGTTTTGC AGCCTCTCCA 
ACTAGATCTA TAAACAGCAA ATATAATTCT ACTGATACAA ATGTCATCAA GGGCACTGCT 
CCCTTGGGCA TGCTGATGGG CAGTCCTGTG CACTTAGAGT CCTCTAATCA GGTTGGTGTG 
ATCCAAAGTA AGTCATGGGA GATGGAGTCG TTAAACACCG GAGACTTTAG CTGCCCAAAT 
TCTGCTGCAC CTGATCAAGA TTCTTCTGTT CAGGGTTTTT GTAATCCTGA AAACAAAGTA 
TTGAGAGAAC AGAATATTGA TTTTGTGTCC ATGCACCAGA CTGAATTGCC GGCAAACACT 
TGTGCTCAGG ATCCAGCGTC CGTCCTTCCT TCCCAGCAAC CTTGCTCTTC GCATAGCCAA 
TCACTTTCAG ATGCTGAATC TATTCCCAGA CATACTTCTT TGTCCTATGT CACCACTCAA 
GAACCAGGTA TTCTACAGCA GAAAAATGCA ATTCAAATGA TCAGTTCTGT TTTGGATACT 
GATACTGAAC CTACAAAAGA TATGGAAAAT ACTTTTGTCC TAACAGACGT TCAGAAGACA 
GATGCCTTTG TCCCAGTGTA CTCTGAAAGC ACTGTTGAAG AAGCAGCACC AAATTTTGAG 
AAAGCTAGTA CTTTGCCAGT ATTGCCATCA GAAAAGGACT TTAATGGAAG TGACGGGTCT 
TCCCAGCCAA ATCCTCATTA TGCATTTAGC AAGCTAATGT TTAAGTCTTC CAGTGGCCAT 
GAGGCTGAAA AAAGCACTTC TGATACTCAG GATACTTCAC ATGAAAAGGA AAACAAACTG 
GAGAGTTTGG TTTTGACTCA CTTAAATGAT AAGTGTGATT CTGATTTATG TGAAATGAAT 
GTAGGGGTGC CAAAAGGAAA CCTGAGTGAA CCAGATAATC CAAAACATTG TCCTGAAAGT 
GAGAAGTGTT TGCTTTCCAT CGAGGATGAG GAGTCACAAC AAAGTACTTT ATCGAGTCTG 
GAGAACCATC CACAGCCATC GGCTCCACCA GAAATGCATA AATACGGACA CTTAGTTAAA 
GTAGAACTAG AGGAAAGTGC TGAAGATGAT AAACCTGAAA GCCAGATCCC TCAAAGGATG 
ACCAGAAACA AAGGAAACAC AGGAGCCAGT CAAAGCAAGC AGCTTCTGGC CAGCTGTGCA 
CTGTTGCCAG ACAAAGACAG TGAGTCTGCG TCCCCTCGAG GAAGAATAAG ATTAACTGAG 
GAGGACGACC CTCAGATTCA CCACCCGCGG AAAAGGAAAG TGTCCCGGGT GCCTCAGCCG 
GTGCAAGTGA GTCCTTCTCT GCTGCAAGCA AAAGAGAAAA CCCAGCAGTC CCTGGCCGCC 
ATTGTAGACT CGCTGAAGCT CGATGAGATT CAGCCATACA GCTCAGAGCG CGCAAATCCC 
TATTTTGAAT ACTTGCACAT AAGAAAAAAG ATTGAAGAAA AGCGCAAGTT GTTATGTAGT 
GTGATCCCTC AGGCACCTCA GTATTATGAT GAATACGTGA CGTTTAATGG GTCGTATCTC 
CTGGACGGAA ACCCCTTAAG CAAGATCTGC ATACCCACAA TTACACCCCC ACCTTCACTG 
TCAGATCCAC TTAAAGAGCT TTTTCGACAA CAGGAAGTTG TCAGGATGAA ACTACGTTTG 
CAGCACAGTA TTGAAAGGGA AAAACTCATT GTATCCAATG AACAGGAGGT TCTTCGGGTT 
CATTACAGAG CTGCAAGGAC ACTGGCAAAT CAGACACTGC CATTCAGCGC ATGCACGGTT 
CTCCTGGATG CGGAAGTGTA CAGCGTGCCC CTGGATGCCC AGTCTGATGA CAGTAAAACT 
TCTGTGAGGG ATCGCTTTAA TGCAAGACAG TTCATGTCAT GGTTACAAGA TGTGGATGAT 
AAATTCGACA AATTAAAGAC CTGTCTTTTA ATGAGGCAAC AGCATGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGTTAGA ATGGCAGCTC AAACTCCAGG AACTTGATCC TGCCACCTAT 
AAATCTATCA GCATCTATGA AATTCAGGAG TTTTACGTTC CGCTGGTTGA TGTTAACGAT 
GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD12 ( XM_002697743.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697789.1 Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X4, mRNA.

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CloneID	OBa11573
Clone ID Related Accession (Same CDS sequence)	XM_002697743.4 , XM_015469151.1 , XM_024984610.1 , XM_002697743.5
Accession Version	XM_002697743.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002697743.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA AACAAAAAAG GAAGCTGGAA ATAAGAAGTC CACTCCAGTT 
AGCATTCTCT TTGGTTACCC ACTCTCAGAG CGAAAGCAGA TGGCACTTCT TATGCAGATG 
ACAGCACGGG ACAACAGTCC AGATTCCACA CCAAATCATC CATCGCAAAC ACCACCAGCC 
CAAAAGAAAA CTCCCAGTTC CTCATCTCGA CAAAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCAGCT ATTCGAGGAG ATGTAAAACA AGTCAAAGAA 
CTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ACGTGGGATA TTATGATGTT GCTAAGATAC TGATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCCGCTAG TAGTGGGCAC 
AGAGATATTG TGAAACTGCT GCTTCGTCAT GGTGGAAACC CTTTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTAGATGT GGCAGAAACA GAGGAGCTGG AGTTGTTGCT GAAAAGAGAG 
GTGCCTTTGT CTGATGATGA TGAGAGTTAC ACAGATTCTG AAGAGGCTCA GTCTGTAAAT 
CCTTCCAGCG TTGATGAAAA TATTGACTCT GAGACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TAGTTCCCAG TAAAGCACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGAAGAGGA AATAAATAAA ATGATTGATG ACAGGCATAT TCTTAGGAAA 
GAACTGCGAA AAGAGAATGA ATCTGAAGTA GAAAAGAGTA GTTTATTTGC AAAACAGGAA 
AAAACCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTTTTGTAT 
TCAAGTTCTG AAAGTTCAGA TGAAGAAATT CTTCAAAACA AAAAGTGTTC TGCTCCATGT 
TCTGTCCCTG AAACATCAAA TTCTGATATT CAAACCAAAA AGGAATATGG AGTTTCAAAT 
GAACACAAAC AGAAAGGCAA AGTTAAAAGA AAATTAAAGA ACCAGAACAA AAATAAAGAG 
AACCAAGAGC TGAAGCAAGA AAAAGAGGGG AAAGAAAATA CCAGAGTAAC AAACTTGACA 
GTTAATACTG CACTAGATTG TTCAGATAAG ACCAGAGATG AGGGGAATTT TAGGAAATCT 
TTTAGCCCGA AAGATGATAC TTCATTACAT TTATTTCATA TTTCCAGTGG TAAATCTCCC 
AAACATTCTT GTGGACTCAG TGAAAAGCAG TCAACACCAC TAAAACAAGA ACATTCTAAA 
ACCTGTTTAT CACCAGGAAG TTCTGAAATG TCATTACAGC CTGATCTTGT TCGGTATGAT 
AATATAGAAT CTGAATTCTT GCCAGAAAGT TCAAGTGTAA AATCTTGTAA GCATAAGGAA 
AAAAACAAAC ATCATAAAGA TTTCCACTTA GAATTTGGTG AAAAGTCAAA TGCCAAAATA 
AAAGATGATG ATCACAGTCC AACATTTGAA AATTCAGATT GCACACTGAA AAAAATGGAT 
AAAGAGGGTA AAACATTAAA AAAACATAAG TTAAAACATA AAGAAAGGGA AAAAGAAAAG 
CATAAAAAAG AAATTGAAGG TGAAAAGGAA AAATACAAAA GTAGGGATGG TACTAAAGAG 
CTGCAACGGA GTGTGGAATT TGATAGAGAG TTTTGGAAAG AGAATTTTTT TAAAAGTGAT 
GAAACCGAAG ATCTATTTTT AAGTATGGAA CATGAGTCCC TAACGTTAGA AAAAAAGTCA 
AAGTTGGAGA AAAACATAAA AGATGATAAA TCAACTAAGG AAAAGCATAT CTCCAAAGAG 
AGAAACTTTA AAGAAGAACG GGAAAAGATT AAAAAGGAAA GTGAGAAATC TTTTAGGGAG 
GAAAAAATAA AAGATTTAAA AGATGACAGA GAGAACGTAC TTACAGATAA AGAAACAGAA 
TTCAGCTCTT TAGGTGTAAG TGCCACTGAA GAGACTATAG GGTTACATTC AGTGGAAAAG 
GAAATAGAAA TTGAAAAGCA TATGAAAGAA AGTAAGGAAA AATCTGAGAA ACGATTTCAA 
ACTAAAGATA AGGACATTGA GAGGGCTGAC AGAAAAAATT CTGAAAAAGA GAAGAAGATA 
AAACATGAGC ATAAGTCAGA GAAAGACAGA TTAGATCCTA GTGAATGTGT CGACAGAATA 
AAAGAAAAGG ACAAGCTATA TTCACATCAC ACAGAAAAAT GCCATAGAGA AGGTGAGAAG 
CTAAAAAACA TTACTACCAT TAAAAAAAAC GATGACAGAG ATAAAAGTAG AGAAAAGATG 
GATAGGAAAC ATGACAAAGA AAAACCTGAG AAGGAGAGAC ATCCAGTAGA GAGCAAAGAG 
AAGCACTTGA TGGAAAAAAA AAACAAGCCA TCAGATAATA GTGACTATAC TAAATCAGAA 
AAAAGCAAAA ATAAAGAAAA AGATAGGGAG GTAGATAAAA AGGACAAATC TAGAGACAGT 
GTAAATATAA GTAATTCCAA ACACTTCCAG GAAGAGAGAA AGTCAAGTAT AACAGACAGT 
AATAAAGCAC AACATGAAAA AACTCTCTCT CTCAAAGAAA AAACAAAAGA TGAACCTTTG 
AGAACTCCTG ATGGAAAAGA AAAAGATAAA AAAGATATAG ATAGATATAA AGAACGAGAC 
AAACATAAGG ATAAAATTCT GAGTAGTTCA CTCAAACTAA AATCTGAAGC AGATAAGCCT 
AAACCGAAGT CATCACCAGC ATCAAAAGAT ACTCGGCCCA AAGAAAAGAG GCTTGTGAAT 
GACGACTTAA TGCAGACCAG CTTTGAACGG ATGCTGAGCC TTAAAGACTT GGAAATAGAG 
CAGTGGCACA GAAAACACAA GGAAAAGATT AAGCAAAAAG AAAAGGAACG GTTGAGAAAT 
CGCAGTTGTT TAGAACTTAA AGTAAAAGAT AAAGAAAAAA CAAAGCATGC ACTAGCTGAG 
TCCAAAAATA AAGAACTTAC TAGATCAAAG AGTTCTGAGT TGACTGATAC TTACATTAAG 
GAGAAACACT GTAAAGATGC TGTAAGTAAT AGATCCCAAT CTGTGGACAC CAAAAATACG 
ATGAGTTTAG GCAAGTCATC CTTTGTTTCA GATAATAGCT TGAACAGGTC TCCTAGATCA 
GAAAGTGAAA AGCCAGGTCT CAGCTCCAGG TCTGTCTCCT TGATTTCAGT GGCTAGCTCA 
GAAGATTCCT GCCTTACTAC AATGACAACC CCAAGGCCTC CAGTTGAGTA TGACTCAGAC 
TTTATGTTAG AGGGTTCGGA TTCCCAAATG TCCTTTTCCC AGTCACCGTT TTTGCCAGTT 
GCCAAATCTC CTGCCCTTCA TGACAGGGAG CTGGATAGCC TGACTGATCT ATCAGAGCGG 
ATTAAAGCAT CGTATGCTAG CAGGCTTCTG ACCTCCCATC TCCGGGCATC TTCTGTCGAA 
GATGCTAAAC TAGTTACAAA TGAAGGGAGA TCAGCTGCAG AAGCCCGAAG ATGTAGCATG 
CCCTCTGTCA TTTGTGAGCA TACAAAGCAA TTCCAAACAA TATCAGAAGA GGGCAGTCAA 
GATGGCTCAG CTGTGCCAAG AGAGATTTGT CCTTCTCCCA AATCTGAGGT ATCCTCCAAC 
GTGCCTGAAA GAGAACTTTT GAGCGTGTCC AACATACATT CCAGTTTTGC AGCCTCTCCA 
ACTAGATCTA TAAACAGCAA ATATAATTCT ACTGATACAA ATGTCATCAA GGGCACTGCT 
CCCTTGGGCA TGCTGATGGG CAGTCCTGTG CACTTAGAGT CCTCTAATCA GGTTGGTGTG 
ATCCAAAGTA AGTCATGGGA GATGGAGTCG TTAAACACCG GAGACTTTAG CTGCCCAAAT 
TCTGCTGCAC CTGATCAAGA TTCTTCTGTT CAGGGTTTTT GTAATCCTGA AAACAAAGTA 
TTGAGAGAAC AGAATATTGA TTTTGTGTCC ATGCACCAGA CTGAATTGCC GGCAAACACT 
TGTGCTCAGG ATCCAGCGTC CGTCCTTCCT TCCCAGCAAC CTTGCTCTTC GCATAGCCAA 
TCACTTTCAG ATGCTGAATC TATTCCCAGA CATACTTCTT TGTCCTATGT CACCACTCAA 
GAACCAGGTA TTCTACAGCA GAAAAATGCA ATTCAAATGA TCAGTTCTGT TTTGGATACT 
GATACTGAAC CTACAAAAGA TATGGAAAAT ACTTTTGTCC TAACAGACGT TCAGAAGACA 
GATGCCTTTG TCCCAGTGTA CTCTGAAAGC ACTGTTGAAG AAGCAGCACC AAATTTTGAG 
AAAGCTAGTA CTTTGCCAGT ATTGCCATCA GAAAAGGACT TTAATGGAAG TGACGGGTCT 
TCCCAGCCAA ATCCTCATTA TGCATTTAGC AAGCTAATGT TTAAGTCTTC CAGTGGCCAT 
GAGGCTGAAA AAAGCACTTC TGATACTCAG GATACTTCAC ATGAAAAGGA AAACAAACTG 
GAGAGTTTGG TTTTGACTCA CTTAAATGAT AAGTGTGATT CTGATTTATG TGAAATGAAT 
GTAGGGGTGC CAAAAGGAAA CCTGAGTGAA CCAGATAATC CAAAACATTG TCCTGAAAGT 
GAGAAGTGTT TGCTTTCCAT CGAGGATGAG GAGTCACAAC AAAGTACTTT ATCGAGTCTG 
GAGAACCATC CACAGCCATC GGCTCCACCA GAAATGCATA AATACGGACA CTTAGTTAAA 
GTAGAACTAG AGGAAAGTGC TGAAGATGAT AAACCTGAAA GCCAGATCCC TCAAAGGATG 
ACCAGAAACA AAGGAAACAC AGGAGCCAGT CAAAGCAAGC AGCTTCTGGC CAGCTGTGCA 
CTGTTGCCAG ACAAAGACAG TGAGTCTGCG TCCCCTCGAG GAAGAATAAG ATTAACTGAG 
GAGGACGACC CTCAGATTCA CCACCCGCGG AAAAGGAAAG TGTCCCGGGT GCCTCAGCCG 
GTGCAAGTGA GTCCTTCTCT GCTGCAAGCA AAAGAGAAAA CCCAGCAGTC CCTGGCCGCC 
ATTGTAGACT CGCTGAAGCT CGATGAGATT CAGCCATACA GCTCAGAGCG CGCAAATCCC 
TATTTTGAAT ACTTGCACAT AAGAAAAAAG ATTGAAGAAA AGCGCAAGTT GTTATGTAGT 
GTGATCCCTC AGGCACCTCA GTATTATGAT GAATACGTGA CGTTTAATGG GTCGTATCTC 
CTGGACGGAA ACCCCTTAAG CAAGATCTGC ATACCCACAA TTACACCCCC ACCTTCACTG 
TCAGATCCAC TTAAAGAGCT TTTTCGACAA CAGGAAGTTG TCAGGATGAA ACTACGTTTG 
CAGCACAGTA TTGAAAGGGA AAAACTCATT GTATCCAATG AACAGGAGGT TCTTCGGGTT 
CATTACAGAG CTGCAAGGAC ACTGGCAAAT CAGACACTGC CATTCAGCGC ATGCACGGTT 
CTCCTGGATG CGGAAGTGTA CAGCGTGCCC CTGGATGCCC AGTCTGATGA CAGTAAAACT 
TCTGTGAGGG ATCGCTTTAA TGCAAGACAG TTCATGTCAT GGTTACAAGA TGTGGATGAT 
AAATTCGACA AATTAAAGAC CTGTCTTTTA ATGAGGCAAC AGCATGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGTTAGA ATGGCAGCTC AAACTCCAGG AACTTGATCC TGCCACCTAT 
AAATCTATCA GCATCTATGA AATTCAGGAG TTTTACGTTC CGCTGGTTGA TGTTAACGAT 
GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697789.1
CDS	225..6308
Translation	MPKSGFTKPVQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIDRNDVGKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTPPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQIVPSKAPLPSALDEYEFKDDDEEEINKMIDDRHILRKELRKENESEVEKSSLFAKQEKTFYPKSFKSKKQKPSRVLYSSSESSDEEILQNKKCSAPCSVPETSNSDIQTKKEYGVSNEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRVTNLTVNTALDCSDKTRDEGNFRKSFSPKDDTSLHLFHISSGKSPKHSCGLSEKQSTPLKQEHSKTCLSPGSSEMSLQPDLVRYDNIESEFLPESSSVKSCKHKEKNKHHKDFHLEFGEKSNAKIKDDDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKSRDGTKELQRSVEFDREFWKENFFKSDETEDLFLSMEHESLTLEKKSKLEKNIKDDKSTKEKHISKERNFKEEREKIKKESEKSFREEKIKDLKDDRENVLTDKETEFSSLGVSATEETIGLHSVEKEIEIEKHMKESKEKSEKRFQTKDKDIERADRKNSEKEKKIKHEHKSEKDRLDPSECVDRIKEKDKLYSHHTEKCHREGEKLKNITTIKKNDDRDKSREKMDRKHDKEKPEKERHPVESKEKHLMEKKNKPSDNSDYTKSEKSKNKEKDREVDKKDKSRDSVNISNSKHFQEERKSSITDSNKAQHEKTLSLKEKTKDEPLRTPDGKEKDKKDIDRYKERDKHKDKILSSSLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHRKHKEKIKQKEKERLRNRSCLELKVKDKEKTKHALAESKNKELTRSKSSELTDTYIKEKHCKDAVSNRSQSVDTKNTMSLGKSSFVSDNSLNRSPRSESEKPGLSSRSVSLISVASSEDSCLTTMTTPRPPVEYDSDFMLEGSDSQMSFSQSPFLPVAKSPALHDRELDSLTDLSERIKASYASRLLTSHLRASSVEDAKLVTNEGRSAAEARRCSMPSVICEHTKQFQTISEEGSQDGSAVPREICPSPKSEVSSNVPERELLSVSNIHSSFAASPTRSINSKYNSTDTNVIKGTAPLGMLMGSPVHLESSNQVGVIQSKSWEMESLNTGDFSCPNSAAPDQDSSVQGFCNPENKVLREQNIDFVSMHQTELPANTCAQDPASVLPSQQPCSSHSQSLSDAESIPRHTSLSYVTTQEPGILQQKNAIQMISSVLDTDTEPTKDMENTFVLTDVQKTDAFVPVYSESTVEEAAPNFEKASTLPVLPSEKDFNGSDGSSQPNPHYAFSKLMFKSSSGHEAEKSTSDTQDTSHEKENKLESLVLTHLNDKCDSDLCEMNVGVPKGNLSEPDNPKHCPESEKCLLSIEDEESQQSTLSSLENHPQPSAPPEMHKYGHLVKVELEESAEDDKPESQIPQRMTRNKGNTGASQSKQLLASCALLPDKDSESASPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYSVPLDAQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI

Target ORF information:

RefSeq Version	XM_002697743.5
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 12 (ANKRD12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697743.5

ORF Insert Sequence:

ATGCCCAAAT CTGGGTTCAC AAAACCAGTT CAGAGTGAAA ATTCTGACAG TGACAGCAAT 
ATGGTAGAAA AACCATATGG AAGAAAGAGT AAAGACAAGA TTGCATCCTA TAGCAAAACT 
CCAAAAATTG ATCGAAATGA TGTGGGCAAG GAGATGAAAG AGAAATCATC CATGAAACGT 
AAACTTCCTT TTACCATCAG CCCATCAAGA AATGAAGAAC GAGATTCAGA CACAGAGAAA 
GAAGGTCCAG AAAAGAAGAA AACAAAAAAG GAAGCTGGAA ATAAGAAGTC CACTCCAGTT 
AGCATTCTCT TTGGTTACCC ACTCTCAGAG CGAAAGCAGA TGGCACTTCT TATGCAGATG 
ACAGCACGGG ACAACAGTCC AGATTCCACA CCAAATCATC CATCGCAAAC ACCACCAGCC 
CAAAAGAAAA CTCCCAGTTC CTCATCTCGA CAAAAAGATA AAGTTAATAA AAGAAATGAA 
CGTGGTGAAA CTCCTTTACA CATGGCAGCT ATTCGAGGAG ATGTAAAACA AGTCAAAGAA 
CTAATAAGTT TAGGGGCAAA TGTGAATGTG AAAGATTTTG CAGGTTGGAC ACCACTGCAT 
GAAGCTTGCA ACGTGGGATA TTATGATGTT GCTAAGATAC TGATAGCAGC TGGAGCAGAT 
GTTAACACAC AAGGATTAGA TGATGACACT CCACTCCATG ATTCCGCTAG TAGTGGGCAC 
AGAGATATTG TGAAACTGCT GCTTCGTCAT GGTGGAAACC CTTTTCAAGC TAATAAACAT 
GGGGAGCGTC CAGTAGATGT GGCAGAAACA GAGGAGCTGG AGTTGTTGCT GAAAAGAGAG 
GTGCCTTTGT CTGATGATGA TGAGAGTTAC ACAGATTCTG AAGAGGCTCA GTCTGTAAAT 
CCTTCCAGCG TTGATGAAAA TATTGACTCT GAGACAGAGA AAGACTCTCT CATCTGTGAA 
AGTAAACAGA TAGTTCCCAG TAAAGCACCT CTTCCATCTG CCCTTGATGA GTATGAGTTC 
AAAGATGATG ATGAAGAGGA AATAAATAAA ATGATTGATG ACAGGCATAT TCTTAGGAAA 
GAACTGCGAA AAGAGAATGA ATCTGAAGTA GAAAAGAGTA GTTTATTTGC AAAACAGGAA 
AAAACCTTCT ATCCTAAATC ATTTAAAAGT AAAAAACAAA AGCCATCTAG GGTTTTGTAT 
TCAAGTTCTG AAAGTTCAGA TGAAGAAATT CTTCAAAACA AAAAGTGTTC TGCTCCATGT 
TCTGTCCCTG AAACATCAAA TTCTGATATT CAAACCAAAA AGGAATATGG AGTTTCAAAT 
GAACACAAAC AGAAAGGCAA AGTTAAAAGA AAATTAAAGA ACCAGAACAA AAATAAAGAG 
AACCAAGAGC TGAAGCAAGA AAAAGAGGGG AAAGAAAATA CCAGAGTAAC AAACTTGACA 
GTTAATACTG CACTAGATTG TTCAGATAAG ACCAGAGATG AGGGGAATTT TAGGAAATCT 
TTTAGCCCGA AAGATGATAC TTCATTACAT TTATTTCATA TTTCCAGTGG TAAATCTCCC 
AAACATTCTT GTGGACTCAG TGAAAAGCAG TCAACACCAC TAAAACAAGA ACATTCTAAA 
ACCTGTTTAT CACCAGGAAG TTCTGAAATG TCATTACAGC CTGATCTTGT TCGGTATGAT 
AATATAGAAT CTGAATTCTT GCCAGAAAGT TCAAGTGTAA AATCTTGTAA GCATAAGGAA 
AAAAACAAAC ATCATAAAGA TTTCCACTTA GAATTTGGTG AAAAGTCAAA TGCCAAAATA 
AAAGATGATG ATCACAGTCC AACATTTGAA AATTCAGATT GCACACTGAA AAAAATGGAT 
AAAGAGGGTA AAACATTAAA AAAACATAAG TTAAAACATA AAGAAAGGGA AAAAGAAAAG 
CATAAAAAAG AAATTGAAGG TGAAAAGGAA AAATACAAAA GTAGGGATGG TACTAAAGAG 
CTGCAACGGA GTGTGGAATT TGATAGAGAG TTTTGGAAAG AGAATTTTTT TAAAAGTGAT 
GAAACCGAAG ATCTATTTTT AAGTATGGAA CATGAGTCCC TAACGTTAGA AAAAAAGTCA 
AAGTTGGAGA AAAACATAAA AGATGATAAA TCAACTAAGG AAAAGCATAT CTCCAAAGAG 
AGAAACTTTA AAGAAGAACG GGAAAAGATT AAAAAGGAAA GTGAGAAATC TTTTAGGGAG 
GAAAAAATAA AAGATTTAAA AGATGACAGA GAGAACGTAC TTACAGATAA AGAAACAGAA 
TTCAGCTCTT TAGGTGTAAG TGCCACTGAA GAGACTATAG GGTTACATTC AGTGGAAAAG 
GAAATAGAAA TTGAAAAGCA TATGAAAGAA AGTAAGGAAA AATCTGAGAA ACGATTTCAA 
ACTAAAGATA AGGACATTGA GAGGGCTGAC AGAAAAAATT CTGAAAAAGA GAAGAAGATA 
AAACATGAGC ATAAGTCAGA GAAAGACAGA TTAGATCCTA GTGAATGTGT CGACAGAATA 
AAAGAAAAGG ACAAGCTATA TTCACATCAC ACAGAAAAAT GCCATAGAGA AGGTGAGAAG 
CTAAAAAACA TTACTACCAT TAAAAAAAAC GATGACAGAG ATAAAAGTAG AGAAAAGATG 
GATAGGAAAC ATGACAAAGA AAAACCTGAG AAGGAGAGAC ATCCAGTAGA GAGCAAAGAG 
AAGCACTTGA TGGAAAAAAA AAACAAGCCA TCAGATAATA GTGACTATAC TAAATCAGAA 
AAAAGCAAAA ATAAAGAAAA AGATAGGGAG GTAGATAAAA AGGACAAATC TAGAGACAGT 
GTAAATATAA GTAATTCCAA ACACTTCCAG GAAGAGAGAA AGTCAAGTAT AACAGACAGT 
AATAAAGCAC AACATGAAAA AACTCTCTCT CTCAAAGAAA AAACAAAAGA TGAACCTTTG 
AGAACTCCTG ATGGAAAAGA AAAAGATAAA AAAGATATAG ATAGATATAA AGAACGAGAC 
AAACATAAGG ATAAAATTCT GAGTAGTTCA CTCAAACTAA AATCTGAAGC AGATAAGCCT 
AAACCGAAGT CATCACCAGC ATCAAAAGAT ACTCGGCCCA AAGAAAAGAG GCTTGTGAAT 
GACGACTTAA TGCAGACCAG CTTTGAACGG ATGCTGAGCC TTAAAGACTT GGAAATAGAG 
CAGTGGCACA GAAAACACAA GGAAAAGATT AAGCAAAAAG AAAAGGAACG GTTGAGAAAT 
CGCAGTTGTT TAGAACTTAA AGTAAAAGAT AAAGAAAAAA CAAAGCATGC ACTAGCTGAG 
TCCAAAAATA AAGAACTTAC TAGATCAAAG AGTTCTGAGT TGACTGATAC TTACATTAAG 
GAGAAACACT GTAAAGATGC TGTAAGTAAT AGATCCCAAT CTGTGGACAC CAAAAATACG 
ATGAGTTTAG GCAAGTCATC CTTTGTTTCA GATAATAGCT TGAACAGGTC TCCTAGATCA 
GAAAGTGAAA AGCCAGGTCT CAGCTCCAGG TCTGTCTCCT TGATTTCAGT GGCTAGCTCA 
GAAGATTCCT GCCTTACTAC AATGACAACC CCAAGGCCTC CAGTTGAGTA TGACTCAGAC 
TTTATGTTAG AGGGTTCGGA TTCCCAAATG TCCTTTTCCC AGTCACCGTT TTTGCCAGTT 
GCCAAATCTC CTGCCCTTCA TGACAGGGAG CTGGATAGCC TGACTGATCT ATCAGAGCGG 
ATTAAAGCAT CGTATGCTAG CAGGCTTCTG ACCTCCCATC TCCGGGCATC TTCTGTCGAA 
GATGCTAAAC TAGTTACAAA TGAAGGGAGA TCAGCTGCAG AAGCCCGAAG ATGTAGCATG 
CCCTCTGTCA TTTGTGAGCA TACAAAGCAA TTCCAAACAA TATCAGAAGA GGGCAGTCAA 
GATGGCTCAG CTGTGCCAAG AGAGATTTGT CCTTCTCCCA AATCTGAGGT ATCCTCCAAC 
GTGCCTGAAA GAGAACTTTT GAGCGTGTCC AACATACATT CCAGTTTTGC AGCCTCTCCA 
ACTAGATCTA TAAACAGCAA ATATAATTCT ACTGATACAA ATGTCATCAA GGGCACTGCT 
CCCTTGGGCA TGCTGATGGG CAGTCCTGTG CACTTAGAGT CCTCTAATCA GGTTGGTGTG 
ATCCAAAGTA AGTCATGGGA GATGGAGTCG TTAAACACCG GAGACTTTAG CTGCCCAAAT 
TCTGCTGCAC CTGATCAAGA TTCTTCTGTT CAGGGTTTTT GTAATCCTGA AAACAAAGTA 
TTGAGAGAAC AGAATATTGA TTTTGTGTCC ATGCACCAGA CTGAATTGCC GGCAAACACT 
TGTGCTCAGG ATCCAGCGTC CGTCCTTCCT TCCCAGCAAC CTTGCTCTTC GCATAGCCAA 
TCACTTTCAG ATGCTGAATC TATTCCCAGA CATACTTCTT TGTCCTATGT CACCACTCAA 
GAACCAGGTA TTCTACAGCA GAAAAATGCA ATTCAAATGA TCAGTTCTGT TTTGGATACT 
GATACTGAAC CTACAAAAGA TATGGAAAAT ACTTTTGTCC TAACAGACGT TCAGAAGACA 
GATGCCTTTG TCCCAGTGTA CTCTGAAAGC ACTGTTGAAG AAGCAGCACC AAATTTTGAG 
AAAGCTAGTA CTTTGCCAGT ATTGCCATCA GAAAAGGACT TTAATGGAAG TGACGGGTCT 
TCCCAGCCAA ATCCTCATTA TGCATTTAGC AAGCTAATGT TTAAGTCTTC CAGTGGCCAT 
GAGGCTGAAA AAAGCACTTC TGATACTCAG GATACTTCAC ATGAAAAGGA AAACAAACTG 
GAGAGTTTGG TTTTGACTCA CTTAAATGAT AAGTGTGATT CTGATTTATG TGAAATGAAT 
GTAGGGGTGC CAAAAGGAAA CCTGAGTGAA CCAGATAATC CAAAACATTG TCCTGAAAGT 
GAGAAGTGTT TGCTTTCCAT CGAGGATGAG GAGTCACAAC AAAGTACTTT ATCGAGTCTG 
GAGAACCATC CACAGCCATC GGCTCCACCA GAAATGCATA AATACGGACA CTTAGTTAAA 
GTAGAACTAG AGGAAAGTGC TGAAGATGAT AAACCTGAAA GCCAGATCCC TCAAAGGATG 
ACCAGAAACA AAGGAAACAC AGGAGCCAGT CAAAGCAAGC AGCTTCTGGC CAGCTGTGCA 
CTGTTGCCAG ACAAAGACAG TGAGTCTGCG TCCCCTCGAG GAAGAATAAG ATTAACTGAG 
GAGGACGACC CTCAGATTCA CCACCCGCGG AAAAGGAAAG TGTCCCGGGT GCCTCAGCCG 
GTGCAAGTGA GTCCTTCTCT GCTGCAAGCA AAAGAGAAAA CCCAGCAGTC CCTGGCCGCC 
ATTGTAGACT CGCTGAAGCT CGATGAGATT CAGCCATACA GCTCAGAGCG CGCAAATCCC 
TATTTTGAAT ACTTGCACAT AAGAAAAAAG ATTGAAGAAA AGCGCAAGTT GTTATGTAGT 
GTGATCCCTC AGGCACCTCA GTATTATGAT GAATACGTGA CGTTTAATGG GTCGTATCTC 
CTGGACGGAA ACCCCTTAAG CAAGATCTGC ATACCCACAA TTACACCCCC ACCTTCACTG 
TCAGATCCAC TTAAAGAGCT TTTTCGACAA CAGGAAGTTG TCAGGATGAA ACTACGTTTG 
CAGCACAGTA TTGAAAGGGA AAAACTCATT GTATCCAATG AACAGGAGGT TCTTCGGGTT 
CATTACAGAG CTGCAAGGAC ACTGGCAAAT CAGACACTGC CATTCAGCGC ATGCACGGTT 
CTCCTGGATG CGGAAGTGTA CAGCGTGCCC CTGGATGCCC AGTCTGATGA CAGTAAAACT 
TCTGTGAGGG ATCGCTTTAA TGCAAGACAG TTCATGTCAT GGTTACAAGA TGTGGATGAT 
AAATTCGACA AATTAAAGAC CTGTCTTTTA ATGAGGCAAC AGCATGAAGC TGCAGCTTTA 
AATGCCGTCC AGAGGTTAGA ATGGCAGCTC AAACTCCAGG AACTTGATCC TGCCACCTAT 
AAATCTATCA GCATCTATGA AATTCAGGAG TTTTACGTTC CGCTGGTTGA TGTTAACGAT 
GACTTTGAAT TGACTCCTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ANKRD12
Entrez Gene ID	534739
Full Name	ankyrin repeat domain 12
General protein information	Preferred Names ankyrin repeat domain 12 Names ankyrin repeat domain-containing protein 12
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Rattus norvegicus (Norway rat)	Ankrd12	NP_001101708.1
	LOC705942	XP_002808200.1
Homo sapiens (human)	ANKRD12	NP_056023.3
Pan troglodytes (chimpanzee)	ANKRD12	XP_001139573.3
Bos taurus (cattle)	ANKRD12	XP_002697790.1
Gallus gallus (chicken)	ANKRD12	XP_423591.3
Canis lupus familiaris (dog)	ANKRD12	XP_537329.2
Mus musculus (house mouse)	Ankrd12	NP_001020743.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ANKRD12 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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