FBXO28 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FBXO28
Entrez Gene ID	23219
Full Name	F-box protein 28
Synonyms	CENP-30,Fbx28
General protein information	Preferred Names F-box protein 28 Names F-box only protein 28 centromere protein 30
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q42.11
Summary	Members of the F-box protein family, such as FBXO28, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008].
Disorder MIM:	609100

mRNA and Protein(s)

mRNA	Protein	Name
NM_001136115.3	NP_001129587.1	F-box only protein 28 isoform b
NM_015176.4	NP_055991.1	F-box only protein 28 isoform a
NM_015176.3	NP_055991.1	F-box only protein 28 isoform a
NM_001136115.2	NP_001129587.1	F-box only protein 28 isoform b

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Fbxo28	NP_001100673.1
Gallus gallus (chicken)	FBXO28	XP_419393.3
Canis lupus familiaris (dog)	FBXO28	XP_854829.1
Danio rerio (zebrafish)	fbxo28	XP_684144.4
Xenopus tropicalis (tropical clawed frog)	fbxo28	NP_001096500.1
Pan troglodytes (chimpanzee)	FBXO28	XP_001135277.1
Homo sapiens (human)	FBXO28	NP_055991.1
	FBXO28	XP_001097187.1
Bos taurus (cattle)	FBXO28	NP_001039890.1
Mus musculus (house mouse)	Fbxo28	NP_780336.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

TP53-based interaction analysis identifies cis-eQTL variants for TP53BP2, FBXO28, and FAM53A that associate with survival and treatment outcome in breast cancer.
Fagerholm R, Khan S, Schmidt MK, Garc?a-Closas M, Heikkil? P, Saarela J, Beesley J, Jamshidi M, Aittom?ki K, Liu J, Ali HR, Andrulis IL, Beckmann MW, Behrens S, Blows FM, Brenner H, Chang-Claude J, Couch FJ, Czene K, Fasching PA, Figueroa J, Floris G, Glendon G, Guo Q, Hall P, Hallberg E, Hamann U, Holleczek B, Hooning MJ, Hopper JL, Jager A, Kabisch M, , Keeman R, Kosma VM, Lambrechts D, Lindblom A, Mannermaa A, Margolin S, Provenzano E, Shah M, Southey MC, Dennis J, Lush M, Michailidou K, Wang Q, Bolla MK, Dunning AM, Easton DF, Pharoah PD, Chenevix-Trench G, Blomqvist C, Nevanlinna H
Oncotarget8(11)18381-18398(2017 Mar)

Fbxo28 promotes mitotic progression and regulates topoisomerase II?-dependent DNA decatenation.
Kratz AS, Richter KT, Schlosser YT, Schmitt M, Shumilov A, Delecluse HJ, Hoffmann I
Cell cycle (Georgetown, Tex.)15(24)3419-3431(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression levels of TP53BP2, FBXO28, and FAM53A genes were associated with patient survival specifically in ER-positive, TP53-mutated tumors.
Title: TP53-based interaction analysis identifies cis-eQTL variants for TP53BP2, FBXO28, and FAM53A that associate with survival and treatment outcome in breast cancer.

Fbxo28 regulates topoisomerase IIalpha decatenation activity and plays an important role in maintaining genomic stability.
Title: Fbxo28 promotes mitotic progression and regulates topoisomerase II?-dependent DNA decatenation.

Refinement of the critical region of 1q41q42 microdeletion syndrome identifies FBXO28 as a candidate causative gene for intellectual disability and seizures.
Title: Refinement of the critical region of 1q41q42 microdeletion syndrome identifies FBXO28 as a candidate causative gene for intellectual disability and seizures.

Study identified F-box protein, FBXO28 that controls MYC-dependent transcription by non-proteolytic ubiquitylation. Depletion of FBXO28 or overexpression of an F-box mutant unable to support MYC ubiquitylation results in an impairment of MYC-driven transcription, transformation and tumourigenesis.
Title: CDK-mediated activation of the SCF(FBXO) (28) ubiquitin ligase promotes MYC-driven transcription and tumourigenesis and predicts poor survival in breast cancer.

The following FBXO28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FBXO28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01302	NM_015176.4 Latest version!	Homo sapiens F-box protein 28 (FBXO28), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu01548	NM_001136115.3 Latest version!	Homo sapiens F-box protein 28 (FBXO28), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu01302	NM_015176.3	Homo sapiens F-box protein 28 (FBXO28), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu01548	NM_001136115.2	Homo sapiens F-box protein 28 (FBXO28), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FBXO28 ( NM_015176.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055991.1 Homo sapiens F-box protein 28 (FBXO28), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01302
Clone ID Related Accession (Same CDS sequence)	NM_015176.4 , NM_015176.3
Accession Version	NM_015176.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	F-box only protein 28 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL390122.16. On Nov 23, 2018 this sequence version replaced NM_015176.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK001628.1, SRR1803615.245511.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000366862.10/ ENSP00000355827.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCAG CGGCGGAGGA GCGGATGGCA GAGGAAGGAG GCGGCGGCCA AGGCGACGGC 
GGTTCCTCTT TGGCCTCCGG CTCTACCCAG CGACAGCCTC CACCGCCCGC GCCACAGCAC 
CCGCAGCCGG GGTCCCAGGC GCTCCCAGCC CCCGCGCTGG CTCCGGACCA GCTGCCTCAA 
AACAACACGC TTGTGGCGCT GCCCATCGTA GCCATCGAGA ACATCCTCAG CTTTATGTCC 
TACGACGAAA TTAGCCAGCT CCGCCTGGTT TGTAAAAGAA TGGACTTGGT CTGCCAGAGA 
ATGTTGAATC AGGGATTTCT GAAAGTGGAG AGGTACCATA ATCTATGTCA GAAACAAGTT 
AAAGCACAAC TCCCAAGGAG AGAGTCAGAA AGGAGAAACC ATTCATTAGC TCGTCATGCA 
GACATTCTTG CTGCTGTTGA AACAAGGCTG TCACTATTAA ATATGACTTT CATGAAATAT 
GTGGATTCCA ATCTCTGTTG CTTCATCCCA GGAAAGGTGA TTGATGAGAT TTATCGTGTG 
TTGAGATATG TCAATTCTAC CAGAGCCCCT CAACGAGCTC ATGAAGTACT TCAAGAATTA 
AGGGATATAT CCTCTATGGC AATGGAGTAC TTTGATGAAA AGATTGTTCC AATTTTAAAG 
AGGAAATTAC CAGGATCAGA TGTTTCTGGA AGACTCATGG GCTCTCCTCC AGTTCCAGGA 
CCGTCTGCAG CCCTAACAAC AATGCAGCTC TTCTCCAAGC AAAATCCTTC AAGACAAGAG 
GTTACCAAAC TCCAGCAGCA GGTTAAAACA AATGGTGCTG GCGTGACTGT TCTCAGGCGT 
GAAATTTCTG AGCTTCGCAC CAAAGTGCAA GAACAGCAAA AACAGCTTCA AGACCAGGAC 
CAGAAACTGC TAGAGCAGAC CCAGATCATA GGTGAACAAA ATGCACGGTT GGCAGAGCTA 
GAACGCAAAC TACGAGAAGT AATGGAAAGT GCTGTAGGAA ATTCCTCAGG GTCCGGGCAG 
AATGAGGAGT CTCCTCGGAA ACGAAAAAAG GCCACGGAAG CCATAGACTC TCTTAGGAAA 
TCTAAACGTC TTCGGAATAG AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055991.1
CDS	20..1126
Translation	MAAAAEERMAEEGGGGQGDGGSSLASGSTQRQPPPPAPQHPQPGSQALPAPALAPDQLPQNNTLVALPIVAIENILSFMSYDEISQLRLVCKRMDLVCQRMLNQGFLKVERYHNLCQKQVKAQLPRRESERRNHSLARHADILAAVETRLSLLNMTFMKYVDSNLCCFIPGKVIDEIYRVLRYVNSTRAPQRAHEVLQELRDISSMAMEYFDEKIVPILKRKLPGSDVSGRLMGSPPVPGPSAALTTMQLFSKQNPSRQEVTKLQQQVKTNGAGVTVLRREISELRTKVQEQQKQLQDQDQKLLEQTQIIGEQNARLAELERKLREVMESAVGNSSGSGQNEESPRKRKKATEAIDSLRKSKRLRNRK

Target ORF information:

RefSeq Version	NM_015176.4
Organism	Homo sapiens(human)
Definition	Homo sapiens F-box protein 28 (FBXO28), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015176.4

ORF Insert Sequence:

ATGGCGGCAG CGGCGGAGGA GCGGATGGCA GAGGAAGGAG GCGGCGGCCA AGGCGACGGC 
GGTTCCTCTT TGGCCTCCGG CTCTACCCAG CGACAGCCTC CACCGCCCGC GCCACAGCAC 
CCGCAGCCGG GGTCCCAGGC GCTCCCAGCC CCCGCGCTGG CTCCGGACCA GCTGCCTCAA 
AACAACACGC TTGTGGCGCT GCCCATCGTA GCCATCGAGA ACATCCTCAG CTTTATGTCC 
TACGACGAAA TTAGCCAGCT CCGCCTGGTT TGTAAAAGAA TGGACTTGGT CTGCCAGAGA 
ATGTTGAATC AGGGATTTCT GAAAGTGGAG AGGTACCATA ATCTATGTCA GAAACAAGTT 
AAAGCACAAC TCCCAAGGAG AGAGTCAGAA AGGAGAAACC ATTCATTAGC TCGTCATGCA 
GACATTCTTG CTGCTGTTGA AACAAGGCTG TCACTATTAA ATATGACTTT CATGAAATAT 
GTGGATTCCA ATCTCTGTTG CTTCATCCCA GGAAAGGTGA TTGATGAGAT TTATCGTGTG 
TTGAGATATG TCAATTCTAC CAGAGCCCCT CAACGAGCTC ATGAAGTACT TCAAGAATTA 
AGGGATATAT CCTCTATGGC AATGGAGTAC TTTGATGAAA AGATTGTTCC AATTTTAAAG 
AGGAAATTAC CAGGATCAGA TGTTTCTGGA AGACTCATGG GCTCTCCTCC AGTTCCAGGA 
CCGTCTGCAG CCCTAACAAC AATGCAGCTC TTCTCCAAGC AAAATCCTTC AAGACAAGAG 
GTTACCAAAC TCCAGCAGCA GGTTAAAACA AATGGTGCTG GCGTGACTGT TCTCAGGCGT 
GAAATTTCTG AGCTTCGCAC CAAAGTGCAA GAACAGCAAA AACAGCTTCA AGACCAGGAC 
CAGAAACTGC TAGAGCAGAC CCAGATCATA GGTGAACAAA ATGCACGGTT GGCAGAGCTA 
GAACGCAAAC TACGAGAAGT AATGGAAAGT GCTGTAGGAA ATTCCTCAGG GTCCGGGCAG 
AATGAGGAGT CTCCTCGGAA ACGAAAAAAG GCCACGGAAG CCATAGACTC TCTTAGGAAA 
TCTAAACGTC TTCGGAATAG AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FBXO28 ( NM_015176.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055991.1 Homo sapiens F-box protein 28 (FBXO28), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01302
Clone ID Related Accession (Same CDS sequence)	NM_015176.4 , NM_015176.3
Accession Version	NM_015176.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Homo sapiens(human)
Product	F-box only protein 28 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB136175.1, BP249147.1, AK001628.1, AL390122.16, AB007952.1 and AI354536.1. On May 24, 2012 this sequence version replaced NM_015176.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.73553.1, SRR1660803.248742.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAG CGGCGGAGGA GCGGATGGCA GAGGAAGGAG GCGGCGGCCA AGGCGACGGC 
GGTTCCTCTT TGGCCTCCGG CTCTACCCAG CGACAGCCTC CACCGCCCGC GCCACAGCAC 
CCGCAGCCGG GGTCCCAGGC GCTCCCAGCC CCCGCGCTGG CTCCGGACCA GCTGCCTCAA 
AACAACACGC TTGTGGCGCT GCCCATCGTA GCCATCGAGA ACATCCTCAG CTTTATGTCC 
TACGACGAAA TTAGCCAGCT CCGCCTGGTT TGTAAAAGAA TGGACTTGGT CTGCCAGAGA 
ATGTTGAATC AGGGATTTCT GAAAGTGGAG AGGTACCATA ATCTATGTCA GAAACAAGTT 
AAAGCACAAC TCCCAAGGAG AGAGTCAGAA AGGAGAAACC ATTCATTAGC TCGTCATGCA 
GACATTCTTG CTGCTGTTGA AACAAGGCTG TCACTATTAA ATATGACTTT CATGAAATAT 
GTGGATTCCA ATCTCTGTTG CTTCATCCCA GGAAAGGTGA TTGATGAGAT TTATCGTGTG 
TTGAGATATG TCAATTCTAC CAGAGCCCCT CAACGAGCTC ATGAAGTACT TCAAGAATTA 
AGGGATATAT CCTCTATGGC AATGGAGTAC TTTGATGAAA AGATTGTTCC AATTTTAAAG 
AGGAAATTAC CAGGATCAGA TGTTTCTGGA AGACTCATGG GCTCTCCTCC AGTTCCAGGA 
CCGTCTGCAG CCCTAACAAC AATGCAGCTC TTCTCCAAGC AAAATCCTTC AAGACAAGAG 
GTTACCAAAC TCCAGCAGCA GGTTAAAACA AATGGTGCTG GCGTGACTGT TCTCAGGCGT 
GAAATTTCTG AGCTTCGCAC CAAAGTGCAA GAACAGCAAA AACAGCTTCA AGACCAGGAC 
CAGAAACTGC TAGAGCAGAC CCAGATCATA GGTGAACAAA ATGCACGGTT GGCAGAGCTA 
GAACGCAAAC TACGAGAAGT AATGGAAAGT GCTGTAGGAA ATTCCTCAGG GTCCGGGCAG 
AATGAGGAGT CTCCTCGGAA ACGAAAAAAG GCCACGGAAG CCATAGACTC TCTTAGGAAA 
TCTAAACGTC TTCGGAATAG AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055991.1
CDS	44..1150
Translation	MAAAAEERMAEEGGGGQGDGGSSLASGSTQRQPPPPAPQHPQPGSQALPAPALAPDQLPQNNTLVALPIVAIENILSFMSYDEISQLRLVCKRMDLVCQRMLNQGFLKVERYHNLCQKQVKAQLPRRESERRNHSLARHADILAAVETRLSLLNMTFMKYVDSNLCCFIPGKVIDEIYRVLRYVNSTRAPQRAHEVLQELRDISSMAMEYFDEKIVPILKRKLPGSDVSGRLMGSPPVPGPSAALTTMQLFSKQNPSRQEVTKLQQQVKTNGAGVTVLRREISELRTKVQEQQKQLQDQDQKLLEQTQIIGEQNARLAELERKLREVMESAVGNSSGSGQNEESPRKRKKATEAIDSLRKSKRLRNRK

Target ORF information:

RefSeq Version	NM_015176.3
Organism	Homo sapiens(human)
Definition	Homo sapiens F-box protein 28 (FBXO28), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015176.3

ORF Insert Sequence:

ATGGCGGCAG CGGCGGAGGA GCGGATGGCA GAGGAAGGAG GCGGCGGCCA AGGCGACGGC 
GGTTCCTCTT TGGCCTCCGG CTCTACCCAG CGACAGCCTC CACCGCCCGC GCCACAGCAC 
CCGCAGCCGG GGTCCCAGGC GCTCCCAGCC CCCGCGCTGG CTCCGGACCA GCTGCCTCAA 
AACAACACGC TTGTGGCGCT GCCCATCGTA GCCATCGAGA ACATCCTCAG CTTTATGTCC 
TACGACGAAA TTAGCCAGCT CCGCCTGGTT TGTAAAAGAA TGGACTTGGT CTGCCAGAGA 
ATGTTGAATC AGGGATTTCT GAAAGTGGAG AGGTACCATA ATCTATGTCA GAAACAAGTT 
AAAGCACAAC TCCCAAGGAG AGAGTCAGAA AGGAGAAACC ATTCATTAGC TCGTCATGCA 
GACATTCTTG CTGCTGTTGA AACAAGGCTG TCACTATTAA ATATGACTTT CATGAAATAT 
GTGGATTCCA ATCTCTGTTG CTTCATCCCA GGAAAGGTGA TTGATGAGAT TTATCGTGTG 
TTGAGATATG TCAATTCTAC CAGAGCCCCT CAACGAGCTC ATGAAGTACT TCAAGAATTA 
AGGGATATAT CCTCTATGGC AATGGAGTAC TTTGATGAAA AGATTGTTCC AATTTTAAAG 
AGGAAATTAC CAGGATCAGA TGTTTCTGGA AGACTCATGG GCTCTCCTCC AGTTCCAGGA 
CCGTCTGCAG CCCTAACAAC AATGCAGCTC TTCTCCAAGC AAAATCCTTC AAGACAAGAG 
GTTACCAAAC TCCAGCAGCA GGTTAAAACA AATGGTGCTG GCGTGACTGT TCTCAGGCGT 
GAAATTTCTG AGCTTCGCAC CAAAGTGCAA GAACAGCAAA AACAGCTTCA AGACCAGGAC 
CAGAAACTGC TAGAGCAGAC CCAGATCATA GGTGAACAAA ATGCACGGTT GGCAGAGCTA 
GAACGCAAAC TACGAGAAGT AATGGAAAGT GCTGTAGGAA ATTCCTCAGG GTCCGGGCAG 
AATGAGGAGT CTCCTCGGAA ACGAAAAAAG GCCACGGAAG CCATAGACTC TCTTAGGAAA 
TCTAAACGTC TTCGGAATAG AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FBXO28 ( NM_001136115.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129587.1 Homo sapiens F-box protein 28 (FBXO28), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01548
Clone ID Related Accession (Same CDS sequence)	NM_001136115.3 , NM_001136115.2
Accession Version	NM_001136115.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	F-box only protein 28 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL390122.16. On May 31, 2019 this sequence version replaced NM_001136115.2. Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (b) is shorter and has a distinct C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.4260.1, AK307892.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAG CGGCGGAGGA GCGGATGGCA GAGGAAGGAG GCGGCGGCCA AGGCGACGGC 
GGTTCCTCTT TGGCCTCCGG CTCTACCCAG CGACAGCCTC CACCGCCCGC GCCACAGCAC 
CCGCAGCCGG GGTCCCAGGC GCTCCCAGCC CCCGCGCTGG CTCCGGACCA GCTGCCTCAA 
AACAACACGC TTGTGGCGCT GCCCATCGTA GCCATCGAGA ACATCCTCAG CTTTATGTCC 
TACGACGAAA TTAGCCAGCT CCGCCTGGTT TGTAAAAGAA TGGACTTGGT CTGCCAGAGA 
ATGTTGAATC AGGGATTTCT GAAAGTGGAG AGGTACCATA ATCTATGTCA GAAACAAGTT 
AAAGCACAAC TCCCAAGGAG AGAGTCAGAA AGGAGAAACC ATTCATTAGC TCGTCATGCA 
GACATTCTTG CTGCTGTTGA AACAAGGCTG TCACTATTAA ATATGACTTT CATGAAATAT 
GTGGATTCCA ATCTCTGTTG CTTCATCCCA GGAAAGTTCC AGGACCGTCT GCAGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129587.1
CDS	20..559
Translation	MAAAAEERMAEEGGGGQGDGGSSLASGSTQRQPPPPAPQHPQPGSQALPAPALAPDQLPQNNTLVALPIVAIENILSFMSYDEISQLRLVCKRMDLVCQRMLNQGFLKVERYHNLCQKQVKAQLPRRESERRNHSLARHADILAAVETRLSLLNMTFMKYVDSNLCCFIPGKFQDRLQP

Target ORF information:

RefSeq Version	NM_001136115.3
Organism	Homo sapiens(human)
Definition	Homo sapiens F-box protein 28 (FBXO28), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136115.3

ORF Insert Sequence:

ATGGCGGCAG CGGCGGAGGA GCGGATGGCA GAGGAAGGAG GCGGCGGCCA AGGCGACGGC 
GGTTCCTCTT TGGCCTCCGG CTCTACCCAG CGACAGCCTC CACCGCCCGC GCCACAGCAC 
CCGCAGCCGG GGTCCCAGGC GCTCCCAGCC CCCGCGCTGG CTCCGGACCA GCTGCCTCAA 
AACAACACGC TTGTGGCGCT GCCCATCGTA GCCATCGAGA ACATCCTCAG CTTTATGTCC 
TACGACGAAA TTAGCCAGCT CCGCCTGGTT TGTAAAAGAA TGGACTTGGT CTGCCAGAGA 
ATGTTGAATC AGGGATTTCT GAAAGTGGAG AGGTACCATA ATCTATGTCA GAAACAAGTT 
AAAGCACAAC TCCCAAGGAG AGAGTCAGAA AGGAGAAACC ATTCATTAGC TCGTCATGCA 
GACATTCTTG CTGCTGTTGA AACAAGGCTG TCACTATTAA ATATGACTTT CATGAAATAT 
GTGGATTCCA ATCTCTGTTG CTTCATCCCA GGAAAGTTCC AGGACCGTCT GCAGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FBXO28 ( NM_001136115.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129587.1 Homo sapiens F-box protein 28 (FBXO28), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01548
Clone ID Related Accession (Same CDS sequence)	NM_001136115.3 , NM_001136115.2
Accession Version	NM_001136115.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	F-box only protein 28 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB136175.1, BP249147.1, AK307892.1, AL390122.16, AB007952.1 and AI354536.1. On May 24, 2012 this sequence version replaced NM_001136115.1. Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (b) is shorter and has a distinct C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.4260.1, SRR1803611.44484.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAG CGGCGGAGGA GCGGATGGCA GAGGAAGGAG GCGGCGGCCA AGGCGACGGC 
GGTTCCTCTT TGGCCTCCGG CTCTACCCAG CGACAGCCTC CACCGCCCGC GCCACAGCAC 
CCGCAGCCGG GGTCCCAGGC GCTCCCAGCC CCCGCGCTGG CTCCGGACCA GCTGCCTCAA 
AACAACACGC TTGTGGCGCT GCCCATCGTA GCCATCGAGA ACATCCTCAG CTTTATGTCC 
TACGACGAAA TTAGCCAGCT CCGCCTGGTT TGTAAAAGAA TGGACTTGGT CTGCCAGAGA 
ATGTTGAATC AGGGATTTCT GAAAGTGGAG AGGTACCATA ATCTATGTCA GAAACAAGTT 
AAAGCACAAC TCCCAAGGAG AGAGTCAGAA AGGAGAAACC ATTCATTAGC TCGTCATGCA 
GACATTCTTG CTGCTGTTGA AACAAGGCTG TCACTATTAA ATATGACTTT CATGAAATAT 
GTGGATTCCA ATCTCTGTTG CTTCATCCCA GGAAAGTTCC AGGACCGTCT GCAGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129587.1
CDS	44..583
Translation	MAAAAEERMAEEGGGGQGDGGSSLASGSTQRQPPPPAPQHPQPGSQALPAPALAPDQLPQNNTLVALPIVAIENILSFMSYDEISQLRLVCKRMDLVCQRMLNQGFLKVERYHNLCQKQVKAQLPRRESERRNHSLARHADILAAVETRLSLLNMTFMKYVDSNLCCFIPGKFQDRLQP

Target ORF information:

RefSeq Version	NM_001136115.2
Organism	Homo sapiens(human)
Definition	Homo sapiens F-box protein 28 (FBXO28), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136115.2

ORF Insert Sequence:

ATGGCGGCAG CGGCGGAGGA GCGGATGGCA GAGGAAGGAG GCGGCGGCCA AGGCGACGGC 
GGTTCCTCTT TGGCCTCCGG CTCTACCCAG CGACAGCCTC CACCGCCCGC GCCACAGCAC 
CCGCAGCCGG GGTCCCAGGC GCTCCCAGCC CCCGCGCTGG CTCCGGACCA GCTGCCTCAA 
AACAACACGC TTGTGGCGCT GCCCATCGTA GCCATCGAGA ACATCCTCAG CTTTATGTCC 
TACGACGAAA TTAGCCAGCT CCGCCTGGTT TGTAAAAGAA TGGACTTGGT CTGCCAGAGA 
ATGTTGAATC AGGGATTTCT GAAAGTGGAG AGGTACCATA ATCTATGTCA GAAACAAGTT 
AAAGCACAAC TCCCAAGGAG AGAGTCAGAA AGGAGAAACC ATTCATTAGC TCGTCATGCA 
GACATTCTTG CTGCTGTTGA AACAAGGCTG TCACTATTAA ATATGACTTT CATGAAATAT 
GTGGATTCCA ATCTCTGTTG CTTCATCCCA GGAAAGTTCC AGGACCGTCT GCAGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

TP53-based interaction analysis identifies cis-eQTL variants for TP53BP2, FBXO28, and FAM53A that associate with survival and treatment outcome in breast cancer.
Oncotarget8(11)18381-18398(2017 Mar)
Fagerholm R,Khan S,Schmidt MK,Garc?a-Closas M,Heikkil? P,Saarela J,Beesley J,Jamshidi M,Aittom?ki K,Liu J,Ali HR,Andrulis IL,Beckmann MW,Behrens S,Blows FM,Brenner H,Chang-Claude J,Couch FJ,Czene K,Fasching PA,Figueroa J,Floris G,Glendon G,Guo Q,Hall P,Hallberg E,Hamann U,Holleczek B,Hooning MJ,Hopper JL,Jager A,Kabisch M,,Keeman R,Kosma VM,Lambrechts D,Lindblom A,Mannermaa A,Margolin S,Provenzano E,Shah M,Southey MC,Dennis J,Lush M,Michailidou K,Wang Q,Bolla MK,Dunning AM,Easton DF,Pharoah PD,Chenevix-Trench G,Blomqvist C,Nevanlinna H

Fbxo28 promotes mitotic progression and regulates topoisomerase II?-dependent DNA decatenation.
Cell cycle (Georgetown, Tex.)15(24)3419-3431(2016 Dec)
Kratz AS,Richter KT,Schlosser YT,Schmitt M,Shumilov A,Delecluse HJ,Hoffmann I

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FBXO28 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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