PHLDA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHLDA1
Entrez Gene ID	22822
Full Name	pleckstrin homology like domain family A member 1
Synonyms	DT1P1B11,PHRIP,TDAG51
General protein information	Preferred Names pleckstrin homology like domain family A member 1 Names pleckstrin homology-like domain family A member 1 PQ-rich protein PQR protein T-cell death-associated gene 51 protein apoptosis-associated nuclear protein proline- and glutamine-rich protein proline- and histidine-rich protein proline-histidine rich protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q21.2
Summary	This gene encodes an evolutionarily conserved proline-histidine rich nuclear protein. The encoded protein may play an important role in the anti-apoptotic effects of insulin-like growth factor-1. [provided by RefSeq, Jul 2008].
Disorder MIM:	605335

mRNA and Protein(s)

mRNA	Protein	Name
NM_007350.3	NP_031376.3	pleckstrin homology-like domain family A member 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PHLDA1	XP_005629218.1
Mus musculus (house mouse)	Phlda1	NP_033370.2
Danio rerio (zebrafish)	phlda1	NP_001006011.1
Homo sapiens (human)	PHLDA1	NP_031376.3
Pan troglodytes (chimpanzee)	PHLDA1	XP_001161528.1
	LOC719036	XP_001117606.2
Rattus norvegicus (Norway rat)	Phlda1	NP_058876.2
Xenopus tropicalis (tropical clawed frog)	phlda1	NP_001015853.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptionally inducible Pleckstrin homology-like domain family A member 1 attenuates ErbB receptor activity by inhibiting receptor oligomerization.
Magi S, Iwamoto K, Yumoto N, Hiroshima M, Nagashima T, Ohki R, Garcia-Munoz A, Volinsky N, Kriegsheim AV, Sako Y, Takahashi K, Kimura S, Kholodenko BN, Okada-Hatakeyama M
The Journal of biological chemistry(2017 Dec)

PHLDA1 (pleckstrin homology-like domain, family A, member 1) knockdown promotes migration and invasion of MCF10A breast epithelial cells.
Bonatto N, Carlini MJ, de Bessa Garcia SA, Nagai MA
Cell adhesion & migration1-10(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our study negatively correlates expression of PHLDA1 and Aurora A in IMR-32 cells and sheds new light on functions of PHLDA1 in the neuroblastoma tumor cells, suggesting its role as a pro-apoptotic protein
Title: Downregulation of the PHLDA1 gene in IMR-32 neuroblastoma cells increases levels of Aurora?A, TRKB and affects proteins involved in apoptosis and autophagy pathways.

Data suggest that high PHLDA1 expression is controlled through an ER-NFkappaB-miR-181 regulatory axis and may contribute to a poor clinical outcome in patients with ER+ breast tumors by enhancing stem-like properties in these tumors.
Title: PHLDA1 expression is controlled by an estrogen receptor-NF?B-miR-181 regulatory loop and is essential for formation of ER+ mammospheres.

Suggest decreased expression of PHLDA1 may play an important role in tumor progression, and may become a new adjunct biomarker in the prognosis in gastric adenocarcinoma.
Title: Correlation of decreased expression of PHLDA1 protein with malignant phenotype of gastric adenocarcinoma.

A role for PHLDA1 as an apoptosis suppressor in oral cancer cells.
Title: Differential susceptibility to hydrogen sulfide-induced apoptosis between PHLDA1-overexpressing oral cancer cell lines and oral keratinocytes: role of PHLDA1 as an apoptosis suppressor.

Data show that downregulation of aurora A kinase by the therapeutic antibody is associated with decreased levels of MYCN protein in cytoplasm, and induced expression of PHLDA1 and P53 proteins.
Title: Targeting GD2 ganglioside and aurora A kinase as a dual strategy leading to cell death in cultures of human neuroblastoma cells.

The following PHLDA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHLDA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu20905	NM_007350.3 Latest version!	Homo sapiens pleckstrin homology like domain family A member 1 (PHLDA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHLDA1 ( NM_007350.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_031376.3 Homo sapiens pleckstrin homology like domain family A member 1 (PHLDA1), mRNA.

Price & Availability↑

CloneID	OHu20905
Clone ID Related Accession (Same CDS sequence)	NM_007350.3
Accession Version	NM_007350.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	pleckstrin homology-like domain family A member 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002699.1 and AC011611.26. On Dec 23, 2005 this sequence version replaced NM_007350.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.47391.1, SRR1660807.230274.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGCGTG CGCCGGCTGC CGAGCGCCTC TTGGAGCTGG GCTTTCCCCC GCGGTGCGGG 
CGCCAGGAGC CGCCTTTTCC GCTGGGTGTC ACTCGGGGGT GGGGAAGATG GCCCATTCAA 
AAGCGCCGCG AGGGGGCCCG GCCAGTGCCC TTCAGTGAGC GCTCGCAAGA GGACGGCAGA 
GGCCCGGCAG CTCGGAGCTC CGGGACCTTG TGGCGCATCA GGACGCGGCT GTCCCTCTGC 
CGGGACCCAG AGCCGCCGCC GCCGCTCTGC CTCCTGCGTG TTAGCCTCCT CTGCGCGCTC 
CGGGCAGGCG GCCGTGGGAG CCGCTGGGGC GAGGACGGCG CGAGGCTGCT GCTGCTGCCC 
CCGGCCCGCG CGGCTGGAAA CGGAGAGGCC GAGCCAAGCG GCGGCCCCTC TTATGCTGGG 
AGGATGCTGG AGAGTAGCGG CTGCAAAGCG CTGAAGGAGG GCGTGCTGGA GAAGCGCAGC 
GACGGGTTGT TGCAGCTCTG GAAGAAAAAG TGTTGCATCC TCACCGAGGA AGGGCTGCTG 
CTTATCCCGC CCAAGCAGCT GCAACACCAG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG 
CAGCAACAAC AGCCCGGGCA GGGGCCGGCC GAGCCGTCCC AACCCAGTGG CCCCGCTGTC 
GCCAGCCTCG AGCCGCCGGT CAAGCTCAAG GAACTGCACT TCTCCAACAT GAAGACCGTG 
GACTGTGTGG AGCGCAAGGG CAAGTACATG TACTTCACTG TGGTGATGGC AGAGGGCAAG 
GAGATCGACT TTCGGTGCCC GCAAGACCAG GGCTGGAACG CCGAGATCAC GCTGCAGATG 
GTGCAGTACA AGAATCGTCA GGCCATCCTG GCGGTCAAAT CCACGCGGCA GAAGCAGCAG 
CACCTGGTCC AGCAGCAGCC CCCCTCGCAG CCGCAGCCGC AGCCGCAGCT CCAGCCCCAA 
CCCCAGCCTC AGCCTCAGCC GCAACCCCAG CCCCAATCAC AACCCCAGCC TCAGCCCCAA 
CCCAAGCCTC AGCCCCAGCA GCTCCACCCG TATCCGCATC CACATCCACA TCCACACTCT 
CATCCTCACT CGCACCCACA CCCTCACCCG CACCCGCATC CGCACCAAAT ACCGCACCCA 
CACCCACAGC CGCACTCGCA GCCGCACGGG CACCGGCTTC TCCGCAGCAC CTCCAACTCT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031376.3
CDS	36..1241
Translation	MRRAPAAERLLELGFPPRCGRQEPPFPLGVTRGWGRWPIQKRREGARPVPFSERSQEDGRGPAARSSGTLWRIRTRLSLCRDPEPPPPLCLLRVSLLCALRAGGRGSRWGEDGARLLLLPPARAAGNGEAEPSGGPSYAGRMLESSGCKALKEGVLEKRSDGLLQLWKKKCCILTEEGLLLIPPKQLQHQQQQQQQQQQQQQQQPGQGPAEPSQPSGPAVASLEPPVKLKELHFSNMKTVDCVERKGKYMYFTVVMAEGKEIDFRCPQDQGWNAEITLQMVQYKNRQAILAVKSTRQKQQHLVQQQPPSQPQPQPQLQPQPQPQPQPQPQPQSQPQPQPQPKPQPQQLHPYPHPHPHPHSHPHSHPHPHPHPHPHQIPHPHPQPHSQPHGHRLLRSTSNSA

Target ORF information:

RefSeq Version	NM_007350.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology like domain family A member 1 (PHLDA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007350.3

ORF Insert Sequence:

ATGAGGCGTG CGCCGGCTGC CGAGCGCCTC TTGGAGCTGG GCTTTCCCCC GCGGTGCGGG 
CGCCAGGAGC CGCCTTTTCC GCTGGGTGTC ACTCGGGGGT GGGGAAGATG GCCCATTCAA 
AAGCGCCGCG AGGGGGCCCG GCCAGTGCCC TTCAGTGAGC GCTCGCAAGA GGACGGCAGA 
GGCCCGGCAG CTCGGAGCTC CGGGACCTTG TGGCGCATCA GGACGCGGCT GTCCCTCTGC 
CGGGACCCAG AGCCGCCGCC GCCGCTCTGC CTCCTGCGTG TTAGCCTCCT CTGCGCGCTC 
CGGGCAGGCG GCCGTGGGAG CCGCTGGGGC GAGGACGGCG CGAGGCTGCT GCTGCTGCCC 
CCGGCCCGCG CGGCTGGAAA CGGAGAGGCC GAGCCAAGCG GCGGCCCCTC TTATGCTGGG 
AGGATGCTGG AGAGTAGCGG CTGCAAAGCG CTGAAGGAGG GCGTGCTGGA GAAGCGCAGC 
GACGGGTTGT TGCAGCTCTG GAAGAAAAAG TGTTGCATCC TCACCGAGGA AGGGCTGCTG 
CTTATCCCGC CCAAGCAGCT GCAACACCAG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG 
CAGCAACAAC AGCCCGGGCA GGGGCCGGCC GAGCCGTCCC AACCCAGTGG CCCCGCTGTC 
GCCAGCCTCG AGCCGCCGGT CAAGCTCAAG GAACTGCACT TCTCCAACAT GAAGACCGTG 
GACTGTGTGG AGCGCAAGGG CAAGTACATG TACTTCACTG TGGTGATGGC AGAGGGCAAG 
GAGATCGACT TTCGGTGCCC GCAAGACCAG GGCTGGAACG CCGAGATCAC GCTGCAGATG 
GTGCAGTACA AGAATCGTCA GGCCATCCTG GCGGTCAAAT CCACGCGGCA GAAGCAGCAG 
CACCTGGTCC AGCAGCAGCC CCCCTCGCAG CCGCAGCCGC AGCCGCAGCT CCAGCCCCAA 
CCCCAGCCTC AGCCTCAGCC GCAACCCCAG CCCCAATCAC AACCCCAGCC TCAGCCCCAA 
CCCAAGCCTC AGCCCCAGCA GCTCCACCCG TATCCGCATC CACATCCACA TCCACACTCT 
CATCCTCACT CGCACCCACA CCCTCACCCG CACCCGCATC CGCACCAAAT ACCGCACCCA 
CACCCACAGC CGCACTCGCA GCCGCACGGG CACCGGCTTC TCCGCAGCAC CTCCAACTCT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptionally inducible Pleckstrin homology-like domain family A member 1 attenuates ErbB receptor activity by inhibiting receptor oligomerization.
The Journal of biological chemistry(2017 Dec)
Magi S,Iwamoto K,Yumoto N,Hiroshima M,Nagashima T,Ohki R,Garcia-Munoz A,Volinsky N,Kriegsheim AV,Sako Y,Takahashi K,Kimura S,Kholodenko BN,Okada-Hatakeyama M

PHLDA1 (pleckstrin homology-like domain, family A, member 1) knockdown promotes migration and invasion of MCF10A breast epithelial cells.
Cell adhesion & migration1-10(2017 Jun)
Bonatto N,Carlini MJ,de Bessa Garcia SA,Nagai MA

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PHLDA1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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