F5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	F5
Entrez Gene ID	2153
Full Name	coagulation factor V
Synonyms	FVL,PCCF,RPRGL1,THPH2
General protein information	Preferred Names coagulation factor V Names coagulation factor V activated protein c cofactor coagulation factor V (proaccelerin, labile factor) coagulation factor V jinjiang A2 domain factor V Leiden
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q24.2
Summary	This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance. [provided by RefSeq, Oct 2008].
Disorder MIM:	612309
Disorder Html:	Factor V deficiency, 227400 (3); {Thrombophilia, susceptibility to, due to factor V Leiden}, 188055 (3); {Stroke, ischemic, susceptibility to}, 601367 (3); {Budd-Chiari syndrome}, 600880 (3); Thrombophilia due to activated protein C resistance, 188055 (3); {Pregnancy loss, recurrent, susceptibility to, 1}, 614389 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000130.4	NP_000121.2	coagulation factor V preproprotein
XM_017000660.1	XP_016856149.1	coagulation factor V isoform X1
NM_000130.5	NP_000121.2	coagulation factor V preproprotein
XM_017000660.2	XP_016856149.1	coagulation factor V isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	F5	NP_776304.1
Rattus norvegicus (Norway rat)	F5	NP_001041343.1
Gallus gallus (chicken)	F5	XP_001231901.3
Danio rerio (zebrafish)	f5	NP_001007209.2
Mus musculus (house mouse)	F5	NP_032002.1
Pan troglodytes (chimpanzee)	F5	XP_513984.4
Homo sapiens (human)	F5	NP_000121.2
	F5	XP_001093072.2
Canis lupus familiaris (dog)	F5	XP_005622605.1
Xenopus tropicalis (tropical clawed frog)	f5	XP_002935820.2

Gene Ontology

The following F5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26030	NM_000130.5 Latest version!	Homo sapiens coagulation factor V (F5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu83340	XM_017000660.2 Latest version!	Homo sapiens coagulation factor V (F5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
View Old Accession Versions >>
OHu26030	NM_000130.4	Homo sapiens coagulation factor V (F5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu83340	XM_017000660.1	Homo sapiens coagulation factor V (F5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F5 ( NM_000130.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000121.2 Homo sapiens coagulation factor V (F5), mRNA.

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CloneID	OHu26030
Clone ID Related Accession (Same CDS sequence)	NM_000130.4 , NM_000130.5
Accession Version	NM_000130.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	coagulation factor V preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU279657.1, AK226079.1, M16967.1 and Z99572.1. On Aug 31, 2019 this sequence version replaced NM_000130.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK226079.1, M16967.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTCCCAG GCTGCCCACG CCTCTGGGTC CTGGTGGTCT TGGGCACCAG CTGGGTAGGC 
TGGGGGAGCC AAGGGACAGA AGCGGCACAG CTAAGGCAGT TCTACGTGGC TGCTCAGGGC 
ATCAGTTGGA GCTACCGACC TGAGCCCACA AACTCAAGTT TGAATCTTTC TGTAACTTCC 
TTTAAGAAAA TTGTCTACAG AGAGTATGAA CCATATTTTA AGAAAGAAAA ACCACAATCT 
ACCATTTCAG GACTTCTTGG GCCTACTTTA TATGCTGAAG TCGGAGACAT CATAAAAGTT 
CACTTTAAAA ATAAGGCAGA TAAGCCCTTG AGCATCCATC CTCAAGGAAT TAGGTACAGT 
AAATTATCAG AAGGTGCTTC TTACCTTGAC CACACATTCC CTGCGGAGAA GATGGACGAC 
GCTGTGGCTC CAGGCCGAGA ATACACCTAT GAATGGAGTA TCAGTGAGGA CAGTGGACCC 
ACCCATGATG ACCCTCCATG CCTCACACAC ATCTATTACT CCCATGAAAA TCTGATCGAG 
GATTTCAACT CGGGGCTGAT TGGGCCCCTG CTTATCTGTA AAAAAGGGAC CCTAACTGAG 
GGTGGGACAC AGAAGACGTT TGACAAGCAA ATCGTGCTAC TATTTGCTGT GTTTGATGAA 
AGCAAGAGCT GGAGCCAGTC ATCATCCCTA ATGTACACAG TCAATGGATA TGTGAATGGG 
ACAATGCCAG ATATAACAGT TTGTGCCCAT GACCACATCA GCTGGCATCT GCTGGGAATG 
AGCTCGGGGC CAGAATTATT CTCCATTCAT TTCAACGGCC AGGTCCTGGA GCAGAACCAT 
CATAAGGTCT CAGCCATCAC CCTTGTCAGT GCTACATCCA CTACCGCAAA TATGACTGTG 
GGCCCAGAGG GAAAGTGGAT CATATCTTCT CTCACCCCAA AACATTTGCA AGCTGGGATG 
CAGGCTTACA TTGACATTAA AAACTGCCCA AAGAAAACCA GGAATCTTAA GAAAATAACT 
CGTGAGCAGA GGCGGCACAT GAAGAGGTGG GAATACTTCA TTGCTGCAGA GGAAGTCATT 
TGGGACTATG CACCTGTAAT ACCAGCGAAT ATGGACAAAA AATACAGGTC TCAGCATTTG 
GATAATTTCT CAAACCAAAT TGGAAAACAT TATAAGAAAG TTATGTACAC ACAGTACGAA 
GATGAGTCCT TCACCAAACA TACAGTGAAT CCCAATATGA AAGAAGATGG GATTTTGGGT 
CCTATTATCA GAGCCCAGGT CAGAGACACA CTCAAAATCG TGTTCAAAAA TATGGCCAGC 
CGCCCCTATA GCATTTACCC TCATGGAGTG ACCTTCTCGC CTTATGAAGA TGAAGTCAAC 
TCTTCTTTCA CCTCAGGCAG GAACAACACC ATGATCAGAG CAGTTCAACC AGGGGAAACC 
TATACTTATA AGTGGAACAT CTTAGAGTTT GATGAACCCA CAGAAAATGA TGCCCAGTGC 
TTAACAAGAC CATACTACAG TGACGTGGAC ATCATGAGAG ACATCGCCTC TGGGCTAATA 
GGACTACTTC TAATCTGTAA GAGCAGATCC CTGGACAGGC GAGGAATACA GAGGGCAGCA 
GACATCGAAC AGCAGGCTGT GTTTGCTGTG TTTGATGAGA ACAAAAGCTG GTACCTTGAG 
GACAACATCA ACAAGTTTTG TGAAAATCCT GATGAGGTGA AACGTGATGA CCCCAAGTTT 
TATGAATCAA ACATCATGAG CACTATCAAT GGCTATGTGC CTGAGAGCAT AACTACTCTT 
GGATTCTGCT TTGATGACAC TGTCCAGTGG CACTTCTGTA GTGTGGGGAC CCAGAATGAA 
ATTTTGACCA TCCACTTCAC TGGGCACTCA TTCATCTATG GAAAGAGGCA TGAGGACACC 
TTGACCCTCT TCCCCATGCG TGGAGAATCT GTGACGGTCA CAATGGATAA TGTTGGAACT 
TGGATGTTAA CTTCCATGAA TTCTAGTCCA AGAAGCAAAA AGCTGAGGCT GAAATTCAGG 
GATGTTAAAT GTATCCCAGA TGATGATGAA GACTCATATG AGATTTTTGA ACCTCCAGAA 
TCTACAGTCA TGGCTACACG GAAAATGCAT GATCGTTTAG AACCTGAAGA TGAAGAGAGT 
GATGCTGACT ATGATTACCA GAACAGACTG GCTGCAGCAT TAGGAATCAG GTCATTCCGA 
AACTCATCAT TGAATCAGGA AGAAGAAGAG TTCAATCTTA CTGCCCTAGC TCTGGAGAAT 
GGCACTGAAT TCGTTTCTTC AAACACAGAT ATAATTGTTG GTTCAAATTA TTCTTCCCCA 
AGTAATATTA GTAAGTTCAC TGTCAATAAC CTTGCAGAAC CTCAGAAAGC CCCTTCTCAC 
CAACAAGCCA CCACAGCTGG TTCCCCACTG AGACACCTCA TTGGCAAGAA CTCAGTTCTC 
AATTCTTCCA CAGCAGAGCA TTCCAGCCCA TATTCTGAAG ACCCTATAGA GGATCCTCTA 
CAGCCAGATG TCACAGGGAT ACGTCTACTT TCACTTGGTG CTGGAGAATT CAAAAGTCAA 
GAACATGCTA AGCATAAGGG ACCCAAGGTA GAAAGAGATC AAGCAGCAAA GCACAGGTTC 
TCCTGGATGA AATTACTAGC ACATAAAGTT GGGAGACACC TAAGCCAAGA CACTGGTTCT 
CCTTCCGGAA TGAGGCCCTG GGAGGACCTT CCTAGCCAAG ACACTGGTTC TCCTTCCAGA 
ATGAGGCCCT GGAAGGACCC TCCTAGTGAT CTGTTACTCT TAAAACAAAG TAACTCATCT 
AAGATTTTGG TTGGGAGATG GCATTTGGCT TCTGAGAAAG GTAGCTATGA AATAATCCAA 
GATACTGATG AAGACACAGC TGTTAACAAT TGGCTGATCA GCCCCCAGAA TGCCTCACGT 
GCTTGGGGAG AAAGCACCCC TCTTGCCAAC AAGCCTGGAA AGCAGAGTGG CCACCCAAAG 
TTTCCTAGAG TTAGACATAA ATCTCTACAA GTAAGACAGG ATGGAGGAAA GAGTAGACTG 
AAGAAAAGCC AGTTTCTCAT TAAGACACGA AAAAAGAAAA AAGAGAAGCA CACACACCAT 
GCTCCTTTAT CTCCGAGGAC CTTTCACCCT CTAAGAAGTG AAGCCTACAA CACATTTTCA 
GAAAGAAGAC TTAAGCATTC GTTGGTGCTT CATAAATCCA ATGAAACATC TCTTCCCACA 
GACCTCAATC AGACATTGCC CTCTATGGAT TTTGGCTGGA TAGCCTCACT TCCTGACCAT 
AATCAGAATT CCTCAAATGA CACTGGTCAG GCAAGCTGTC CTCCAGGTCT TTATCAGACA 
GTGCCCCCAG AGGAACACTA TCAAACATTC CCCATTCAAG ACCCTGATCA AATGCACTCT 
ACTTCAGACC CCAGTCACAG ATCCTCTTCT CCAGAGCTCA GTGAAATGCT TGAGTATGAC 
CGAAGTCACA AGTCCTTCCC CACAGATATA AGTCAAATGT CCCCTTCCTC AGAACATGAA 
GTCTGGCAGA CAGTCATCTC TCCAGACCTC AGCCAGGTGA CCCTCTCTCC AGAACTCAGC 
CAGACAAACC TCTCTCCAGA CCTCAGCCAC ACGACTCTCT CTCCAGAACT CATTCAGAGA 
AACCTTTCCC CAGCCCTCGG TCAGATGCCC ATTTCTCCAG ACCTCAGCCA TACAACCCTT 
TCTCCAGACC TCAGCCATAC AACCCTTTCT TTAGACCTCA GCCAGACAAA CCTCTCTCCA 
GAACTCAGTC AGACAAACCT TTCTCCAGCC CTCGGTCAGA TGCCCCTTTC TCCAGACCTC 
AGCCATACAA CCCTTTCTCT AGACTTCAGC CAGACAAACC TCTCTCCAGA ACTCAGCCAT 
ATGACTCTCT CTCCAGAACT CAGTCAGACA AACCTTTCCC CAGCCCTCGG TCAGATGCCC 
ATTTCTCCAG ACCTCAGCCA TACAACCCTT TCTCTAGACT TCAGCCAGAC AAACCTCTCT 
CCAGAACTCA GTCAAACAAA CCTTTCCCCA GCCCTCGGTC AGATGCCCCT TTCTCCAGAC 
CCCAGCCATA CAACCCTTTC TCTAGACCTC AGCCAGACAA ACCTCTCTCC AGAACTCAGT 
CAGACAAACC TTTCCCCAGA CCTCAGTGAG ATGCCCCTCT TTGCAGATCT CAGTCAAATT 
CCCCTTACCC CAGACCTCGA CCAGATGACA CTTTCTCCAG ACCTTGGTGA GACAGATCTT 
TCCCCAAACT TTGGTCAGAT GTCCCTTTCC CCAGACCTCA GCCAGGTGAC TCTCTCTCCA 
GACATCAGTG ACACCACCCT TCTCCCGGAT CTCAGCCAGA TATCACCTCC TCCAGACCTT 
GATCAGATAT TCTACCCTTC TGAATCTAGT CAGTCATTGC TTCTTCAAGA ATTTAATGAG 
TCTTTTCCTT ATCCAGACCT TGGTCAGATG CCATCTCCTT CATCTCCTAC TCTCAATGAT 
ACTTTTCTAT CAAAGGAATT TAATCCACTG GTTATAGTGG GCCTCAGTAA AGATGGTACA 
GATTACATTG AGATCATTCC AAAGGAAGAG GTCCAGAGCA GTGAAGATGA CTATGCTGAA 
ATTGATTATG TGCCCTATGA TGACCCCTAC AAAACTGATG TTAGGACAAA CATCAACTCC 
TCCAGAGATC CTGACAACAT TGCAGCATGG TACCTCCGCA GCAACAATGG AAACAGAAGA 
AATTATTACA TTGCTGCTGA AGAAATATCC TGGGATTATT CAGAATTTGT ACAAAGGGAA 
ACAGATATTG AAGACTCTGA TGATATTCCA GAAGATACCA CATATAAGAA AGTAGTTTTT 
CGAAAGTACC TCGACAGCAC TTTTACCAAA CGTGATCCTC GAGGGGAGTA TGAAGAGCAT 
CTCGGAATTC TTGGTCCTAT TATCAGAGCT GAAGTGGATG ATGTTATCCA AGTTCGTTTT 
AAAAATTTAG CATCCAGACC GTATTCTCTA CATGCCCATG GACTTTCCTA TGAAAAATCA 
TCAGAGGGAA AGACTTATGA AGATGACTCT CCTGAATGGT TTAAGGAAGA TAATGCTGTT 
CAGCCAAATA GCAGTTATAC CTACGTATGG CATGCCACTG AGCGATCAGG GCCAGAAAGT 
CCTGGCTCTG CCTGTCGGGC TTGGGCCTAC TACTCAGCTG TGAACCCAGA AAAAGATATT 
CACTCAGGCT TGATAGGTCC CCTCCTAATC TGCCAAAAAG GAATACTACA TAAGGACAGC 
AACATGCCTA TGGACATGAG AGAATTTGTC TTACTATTTA TGACCTTTGA TGAAAAGAAG 
AGCTGGTACT ATGAAAAGAA GTCCCGAAGT TCTTGGAGAC TCACATCCTC AGAAATGAAA 
AAATCCCATG AGTTTCACGC CATTAATGGG ATGATCTACA GCTTGCCTGG CCTGAAAATG 
TATGAGCAAG AGTGGGTGAG GTTACACCTG CTGAACATAG GCGGCTCCCA AGACATTCAC 
GTGGTTCACT TTCACGGCCA GACCTTGCTG GAAAATGGCA ATAAACAGCA CCAGTTAGGG 
GTCTGGCCCC TTCTGCCTGG TTCATTTAAA ACTCTTGAAA TGAAGGCATC AAAACCTGGC 
TGGTGGCTCC TAAACACAGA GGTTGGAGAA AACCAGAGAG CAGGGATGCA AACGCCATTT 
CTTATCATGG ACAGAGACTG TAGGATGCCA ATGGGACTAA GCACTGGTAT CATATCTGAT 
TCACAGATCA AGGCTTCAGA GTTTCTGGGT TACTGGGAGC CCAGATTAGC AAGATTAAAC 
AATGGTGGAT CTTATAATGC TTGGAGTGTA GAAAAACTTG CAGCAGAATT TGCCTCTAAA 
CCTTGGATCC AGGTGGACAT GCAAAAGGAA GTCATAATCA CAGGGATCCA GACCCAAGGT 
GCCAAACACT ACCTGAAGTC CTGCTATACC ACAGAGTTCT ATGTAGCTTA CAGTTCCAAC 
CAGATCAACT GGCAGATCTT CAAAGGGAAC AGCACAAGGA ATGTGATGTA TTTTAATGGC 
AATTCAGATG CCTCTACAAT AAAAGAGAAT CAGTTTGACC CACCTATTGT GGCTAGATAT 
ATTAGGATCT CTCCAACTCG AGCCTATAAC AGACCTACCC TTCGATTGGA ACTGCAAGGT 
TGTGAGGTAA ATGGATGTTC CACACCCCTG GGTATGGAAA ATGGAAAGAT AGAAAACAAG 
CAAATCACAG CTTCTTCGTT TAAGAAATCT TGGTGGGGAG ATTACTGGGA ACCCTTCCGT 
GCCCGTCTGA ATGCCCAGGG ACGTGTGAAT GCCTGGCAAG CCAAGGCAAA CAACAATAAG 
CAGTGGCTAG AAATTGATCT ACTCAAGATC AAGAAGATAA CGGCAATTAT AACACAGGGC 
TGCAAGTCTC TGTCCTCTGA AATGTATGTA AAGAGCTATA CCATCCACTA CAGTGAGCAG 
GGAGTGGAAT GGAAACCATA CAGGCTGAAA TCCTCCATGG TGGACAAGAT TTTTGAAGGA 
AATACTAATA CCAAAGGACA TGTGAAGAAC TTTTTCAACC CCCCAATCAT TTCCAGGTTT 
ATCCGTGTCA TTCCTAAAAC ATGGAATCAA AGTATTGCAC TTCGCCTGGA ACTCTTTGGC 
TGTGATATTT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000121.2
CDS	96..6770
Translation	MFPGCPRLWVLVVLGTSWVGWGSQGTEAAQLRQFYVAAQGISWSYRPEPTNSSLNLSVTSFKKIVYREYEPYFKKEKPQSTISGLLGPTLYAEVGDIIKVHFKNKADKPLSIHPQGIRYSKLSEGASYLDHTFPAEKMDDAVAPGREYTYEWSISEDSGPTHDDPPCLTHIYYSHENLIEDFNSGLIGPLLICKKGTLTEGGTQKTFDKQIVLLFAVFDESKSWSQSSSLMYTVNGYVNGTMPDITVCAHDHISWHLLGMSSGPELFSIHFNGQVLEQNHHKVSAITLVSATSTTANMTVGPEGKWIISSLTPKHLQAGMQAYIDIKNCPKKTRNLKKITREQRRHMKRWEYFIAAEEVIWDYAPVIPANMDKKYRSQHLDNFSNQIGKHYKKVMYTQYEDESFTKHTVNPNMKEDGILGPIIRAQVRDTLKIVFKNMASRPYSIYPHGVTFSPYEDEVNSSFTSGRNNTMIRAVQPGETYTYKWNILEFDEPTENDAQCLTRPYYSDVDIMRDIASGLIGLLLICKSRSLDRRGIQRAADIEQQAVFAVFDENKSWYLEDNINKFCENPDEVKRDDPKFYESNIMSTINGYVPESITTLGFCFDDTVQWHFCSVGTQNEILTIHFTGHSFIYGKRHEDTLTLFPMRGESVTVTMDNVGTWMLTSMNSSPRSKKLRLKFRDVKCIPDDDEDSYEIFEPPESTVMATRKMHDRLEPEDEESDADYDYQNRLAAALGIRSFRNSSLNQEEEEFNLTALALENGTEFVSSNTDIIVGSNYSSPSNISKFTVNNLAEPQKAPSHQQATTAGSPLRHLIGKNSVLNSSTAEHSSPYSEDPIEDPLQPDVTGIRLLSLGAGEFKSQEHAKHKGPKVERDQAAKHRFSWMKLLAHKVGRHLSQDTGSPSGMRPWEDLPSQDTGSPSRMRPWKDPPSDLLLLKQSNSSKILVGRWHLASEKGSYEIIQDTDEDTAVNNWLISPQNASRAWGESTPLANKPGKQSGHPKFPRVRHKSLQVRQDGGKSRLKKSQFLIKTRKKKKEKHTHHAPLSPRTFHPLRSEAYNTFSERRLKHSLVLHKSNETSLPTDLNQTLPSMDFGWIASLPDHNQNSSNDTGQASCPPGLYQTVPPEEHYQTFPIQDPDQMHSTSDPSHRSSSPELSEMLEYDRSHKSFPTDISQMSPSSEHEVWQTVISPDLSQVTLSPELSQTNLSPDLSHTTLSPELIQRNLSPALGQMPISPDLSHTTLSPDLSHTTLSLDLSQTNLSPELSQTNLSPALGQMPLSPDLSHTTLSLDFSQTNLSPELSHMTLSPELSQTNLSPALGQMPISPDLSHTTLSLDFSQTNLSPELSQTNLSPALGQMPLSPDPSHTTLSLDLSQTNLSPELSQTNLSPDLSEMPLFADLSQIPLTPDLDQMTLSPDLGETDLSPNFGQMSLSPDLSQVTLSPDISDTTLLPDLSQISPPPDLDQIFYPSESSQSLLLQEFNESFPYPDLGQMPSPSSPTLNDTFLSKEFNPLVIVGLSKDGTDYIEIIPKEEVQSSEDDYAEIDYVPYDDPYKTDVRTNINSSRDPDNIAAWYLRSNNGNRRNYYIAAEEISWDYSEFVQRETDIEDSDDIPEDTTYKKVVFRKYLDSTFTKRDPRGEYEEHLGILGPIIRAEVDDVIQVRFKNLASRPYSLHAHGLSYEKSSEGKTYEDDSPEWFKEDNAVQPNSSYTYVWHATERSGPESPGSACRAWAYYSAVNPEKDIHSGLIGPLLICQKGILHKDSNMPMDMREFVLLFMTFDEKKSWYYEKKSRSSWRLTSSEMKKSHEFHAINGMIYSLPGLKMYEQEWVRLHLLNIGGSQDIHVVHFHGQTLLENGNKQHQLGVWPLLPGSFKTLEMKASKPGWWLLNTEVGENQRAGMQTPFLIMDRDCRMPMGLSTGIISDSQIKASEFLGYWEPRLARLNNGGSYNAWSVEKLAAEFASKPWIQVDMQKEVIITGIQTQGAKHYLKSCYTTEFYVAYSSNQINWQIFKGNSTRNVMYFNGNSDASTIKENQFDPPIVARYIRISPTRAYNRPTLRLELQGCEVNGCSTPLGMENGKIENKQITASSFKKSWWGDYWEPFRARLNAQGRVNAWQAKANNNKQWLEIDLLKIKKITAIITQGCKSLSSEMYVKSYTIHYSEQGVEWKPYRLKSSMVDKIFEGNTNTKGHVKNFFNPPIISRFIRVIPKTWNQSIALRLELFGCDIY

Target ORF information:

RefSeq Version	NM_000130.5
Organism	Homo sapiens(human)
Definition	Homo sapiens coagulation factor V (F5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000130.5

ORF Insert Sequence:

ATGTTCCCAG GCTGCCCACG CCTCTGGGTC CTGGTGGTCT TGGGCACCAG CTGGGTAGGC 
TGGGGGAGCC AAGGGACAGA AGCGGCACAG CTAAGGCAGT TCTACGTGGC TGCTCAGGGC 
ATCAGTTGGA GCTACCGACC TGAGCCCACA AACTCAAGTT TGAATCTTTC TGTAACTTCC 
TTTAAGAAAA TTGTCTACAG AGAGTATGAA CCATATTTTA AGAAAGAAAA ACCACAATCT 
ACCATTTCAG GACTTCTTGG GCCTACTTTA TATGCTGAAG TCGGAGACAT CATAAAAGTT 
CACTTTAAAA ATAAGGCAGA TAAGCCCTTG AGCATCCATC CTCAAGGAAT TAGGTACAGT 
AAATTATCAG AAGGTGCTTC TTACCTTGAC CACACATTCC CTGCGGAGAA GATGGACGAC 
GCTGTGGCTC CAGGCCGAGA ATACACCTAT GAATGGAGTA TCAGTGAGGA CAGTGGACCC 
ACCCATGATG ACCCTCCATG CCTCACACAC ATCTATTACT CCCATGAAAA TCTGATCGAG 
GATTTCAACT CGGGGCTGAT TGGGCCCCTG CTTATCTGTA AAAAAGGGAC CCTAACTGAG 
GGTGGGACAC AGAAGACGTT TGACAAGCAA ATCGTGCTAC TATTTGCTGT GTTTGATGAA 
AGCAAGAGCT GGAGCCAGTC ATCATCCCTA ATGTACACAG TCAATGGATA TGTGAATGGG 
ACAATGCCAG ATATAACAGT TTGTGCCCAT GACCACATCA GCTGGCATCT GCTGGGAATG 
AGCTCGGGGC CAGAATTATT CTCCATTCAT TTCAACGGCC AGGTCCTGGA GCAGAACCAT 
CATAAGGTCT CAGCCATCAC CCTTGTCAGT GCTACATCCA CTACCGCAAA TATGACTGTG 
GGCCCAGAGG GAAAGTGGAT CATATCTTCT CTCACCCCAA AACATTTGCA AGCTGGGATG 
CAGGCTTACA TTGACATTAA AAACTGCCCA AAGAAAACCA GGAATCTTAA GAAAATAACT 
CGTGAGCAGA GGCGGCACAT GAAGAGGTGG GAATACTTCA TTGCTGCAGA GGAAGTCATT 
TGGGACTATG CACCTGTAAT ACCAGCGAAT ATGGACAAAA AATACAGGTC TCAGCATTTG 
GATAATTTCT CAAACCAAAT TGGAAAACAT TATAAGAAAG TTATGTACAC ACAGTACGAA 
GATGAGTCCT TCACCAAACA TACAGTGAAT CCCAATATGA AAGAAGATGG GATTTTGGGT 
CCTATTATCA GAGCCCAGGT CAGAGACACA CTCAAAATCG TGTTCAAAAA TATGGCCAGC 
CGCCCCTATA GCATTTACCC TCATGGAGTG ACCTTCTCGC CTTATGAAGA TGAAGTCAAC 
TCTTCTTTCA CCTCAGGCAG GAACAACACC ATGATCAGAG CAGTTCAACC AGGGGAAACC 
TATACTTATA AGTGGAACAT CTTAGAGTTT GATGAACCCA CAGAAAATGA TGCCCAGTGC 
TTAACAAGAC CATACTACAG TGACGTGGAC ATCATGAGAG ACATCGCCTC TGGGCTAATA 
GGACTACTTC TAATCTGTAA GAGCAGATCC CTGGACAGGC GAGGAATACA GAGGGCAGCA 
GACATCGAAC AGCAGGCTGT GTTTGCTGTG TTTGATGAGA ACAAAAGCTG GTACCTTGAG 
GACAACATCA ACAAGTTTTG TGAAAATCCT GATGAGGTGA AACGTGATGA CCCCAAGTTT 
TATGAATCAA ACATCATGAG CACTATCAAT GGCTATGTGC CTGAGAGCAT AACTACTCTT 
GGATTCTGCT TTGATGACAC TGTCCAGTGG CACTTCTGTA GTGTGGGGAC CCAGAATGAA 
ATTTTGACCA TCCACTTCAC TGGGCACTCA TTCATCTATG GAAAGAGGCA TGAGGACACC 
TTGACCCTCT TCCCCATGCG TGGAGAATCT GTGACGGTCA CAATGGATAA TGTTGGAACT 
TGGATGTTAA CTTCCATGAA TTCTAGTCCA AGAAGCAAAA AGCTGAGGCT GAAATTCAGG 
GATGTTAAAT GTATCCCAGA TGATGATGAA GACTCATATG AGATTTTTGA ACCTCCAGAA 
TCTACAGTCA TGGCTACACG GAAAATGCAT GATCGTTTAG AACCTGAAGA TGAAGAGAGT 
GATGCTGACT ATGATTACCA GAACAGACTG GCTGCAGCAT TAGGAATCAG GTCATTCCGA 
AACTCATCAT TGAATCAGGA AGAAGAAGAG TTCAATCTTA CTGCCCTAGC TCTGGAGAAT 
GGCACTGAAT TCGTTTCTTC AAACACAGAT ATAATTGTTG GTTCAAATTA TTCTTCCCCA 
AGTAATATTA GTAAGTTCAC TGTCAATAAC CTTGCAGAAC CTCAGAAAGC CCCTTCTCAC 
CAACAAGCCA CCACAGCTGG TTCCCCACTG AGACACCTCA TTGGCAAGAA CTCAGTTCTC 
AATTCTTCCA CAGCAGAGCA TTCCAGCCCA TATTCTGAAG ACCCTATAGA GGATCCTCTA 
CAGCCAGATG TCACAGGGAT ACGTCTACTT TCACTTGGTG CTGGAGAATT CAAAAGTCAA 
GAACATGCTA AGCATAAGGG ACCCAAGGTA GAAAGAGATC AAGCAGCAAA GCACAGGTTC 
TCCTGGATGA AATTACTAGC ACATAAAGTT GGGAGACACC TAAGCCAAGA CACTGGTTCT 
CCTTCCGGAA TGAGGCCCTG GGAGGACCTT CCTAGCCAAG ACACTGGTTC TCCTTCCAGA 
ATGAGGCCCT GGAAGGACCC TCCTAGTGAT CTGTTACTCT TAAAACAAAG TAACTCATCT 
AAGATTTTGG TTGGGAGATG GCATTTGGCT TCTGAGAAAG GTAGCTATGA AATAATCCAA 
GATACTGATG AAGACACAGC TGTTAACAAT TGGCTGATCA GCCCCCAGAA TGCCTCACGT 
GCTTGGGGAG AAAGCACCCC TCTTGCCAAC AAGCCTGGAA AGCAGAGTGG CCACCCAAAG 
TTTCCTAGAG TTAGACATAA ATCTCTACAA GTAAGACAGG ATGGAGGAAA GAGTAGACTG 
AAGAAAAGCC AGTTTCTCAT TAAGACACGA AAAAAGAAAA AAGAGAAGCA CACACACCAT 
GCTCCTTTAT CTCCGAGGAC CTTTCACCCT CTAAGAAGTG AAGCCTACAA CACATTTTCA 
GAAAGAAGAC TTAAGCATTC GTTGGTGCTT CATAAATCCA ATGAAACATC TCTTCCCACA 
GACCTCAATC AGACATTGCC CTCTATGGAT TTTGGCTGGA TAGCCTCACT TCCTGACCAT 
AATCAGAATT CCTCAAATGA CACTGGTCAG GCAAGCTGTC CTCCAGGTCT TTATCAGACA 
GTGCCCCCAG AGGAACACTA TCAAACATTC CCCATTCAAG ACCCTGATCA AATGCACTCT 
ACTTCAGACC CCAGTCACAG ATCCTCTTCT CCAGAGCTCA GTGAAATGCT TGAGTATGAC 
CGAAGTCACA AGTCCTTCCC CACAGATATA AGTCAAATGT CCCCTTCCTC AGAACATGAA 
GTCTGGCAGA CAGTCATCTC TCCAGACCTC AGCCAGGTGA CCCTCTCTCC AGAACTCAGC 
CAGACAAACC TCTCTCCAGA CCTCAGCCAC ACGACTCTCT CTCCAGAACT CATTCAGAGA 
AACCTTTCCC CAGCCCTCGG TCAGATGCCC ATTTCTCCAG ACCTCAGCCA TACAACCCTT 
TCTCCAGACC TCAGCCATAC AACCCTTTCT TTAGACCTCA GCCAGACAAA CCTCTCTCCA 
GAACTCAGTC AGACAAACCT TTCTCCAGCC CTCGGTCAGA TGCCCCTTTC TCCAGACCTC 
AGCCATACAA CCCTTTCTCT AGACTTCAGC CAGACAAACC TCTCTCCAGA ACTCAGCCAT 
ATGACTCTCT CTCCAGAACT CAGTCAGACA AACCTTTCCC CAGCCCTCGG TCAGATGCCC 
ATTTCTCCAG ACCTCAGCCA TACAACCCTT TCTCTAGACT TCAGCCAGAC AAACCTCTCT 
CCAGAACTCA GTCAAACAAA CCTTTCCCCA GCCCTCGGTC AGATGCCCCT TTCTCCAGAC 
CCCAGCCATA CAACCCTTTC TCTAGACCTC AGCCAGACAA ACCTCTCTCC AGAACTCAGT 
CAGACAAACC TTTCCCCAGA CCTCAGTGAG ATGCCCCTCT TTGCAGATCT CAGTCAAATT 
CCCCTTACCC CAGACCTCGA CCAGATGACA CTTTCTCCAG ACCTTGGTGA GACAGATCTT 
TCCCCAAACT TTGGTCAGAT GTCCCTTTCC CCAGACCTCA GCCAGGTGAC TCTCTCTCCA 
GACATCAGTG ACACCACCCT TCTCCCGGAT CTCAGCCAGA TATCACCTCC TCCAGACCTT 
GATCAGATAT TCTACCCTTC TGAATCTAGT CAGTCATTGC TTCTTCAAGA ATTTAATGAG 
TCTTTTCCTT ATCCAGACCT TGGTCAGATG CCATCTCCTT CATCTCCTAC TCTCAATGAT 
ACTTTTCTAT CAAAGGAATT TAATCCACTG GTTATAGTGG GCCTCAGTAA AGATGGTACA 
GATTACATTG AGATCATTCC AAAGGAAGAG GTCCAGAGCA GTGAAGATGA CTATGCTGAA 
ATTGATTATG TGCCCTATGA TGACCCCTAC AAAACTGATG TTAGGACAAA CATCAACTCC 
TCCAGAGATC CTGACAACAT TGCAGCATGG TACCTCCGCA GCAACAATGG AAACAGAAGA 
AATTATTACA TTGCTGCTGA AGAAATATCC TGGGATTATT CAGAATTTGT ACAAAGGGAA 
ACAGATATTG AAGACTCTGA TGATATTCCA GAAGATACCA CATATAAGAA AGTAGTTTTT 
CGAAAGTACC TCGACAGCAC TTTTACCAAA CGTGATCCTC GAGGGGAGTA TGAAGAGCAT 
CTCGGAATTC TTGGTCCTAT TATCAGAGCT GAAGTGGATG ATGTTATCCA AGTTCGTTTT 
AAAAATTTAG CATCCAGACC GTATTCTCTA CATGCCCATG GACTTTCCTA TGAAAAATCA 
TCAGAGGGAA AGACTTATGA AGATGACTCT CCTGAATGGT TTAAGGAAGA TAATGCTGTT 
CAGCCAAATA GCAGTTATAC CTACGTATGG CATGCCACTG AGCGATCAGG GCCAGAAAGT 
CCTGGCTCTG CCTGTCGGGC TTGGGCCTAC TACTCAGCTG TGAACCCAGA AAAAGATATT 
CACTCAGGCT TGATAGGTCC CCTCCTAATC TGCCAAAAAG GAATACTACA TAAGGACAGC 
AACATGCCTA TGGACATGAG AGAATTTGTC TTACTATTTA TGACCTTTGA TGAAAAGAAG 
AGCTGGTACT ATGAAAAGAA GTCCCGAAGT TCTTGGAGAC TCACATCCTC AGAAATGAAA 
AAATCCCATG AGTTTCACGC CATTAATGGG ATGATCTACA GCTTGCCTGG CCTGAAAATG 
TATGAGCAAG AGTGGGTGAG GTTACACCTG CTGAACATAG GCGGCTCCCA AGACATTCAC 
GTGGTTCACT TTCACGGCCA GACCTTGCTG GAAAATGGCA ATAAACAGCA CCAGTTAGGG 
GTCTGGCCCC TTCTGCCTGG TTCATTTAAA ACTCTTGAAA TGAAGGCATC AAAACCTGGC 
TGGTGGCTCC TAAACACAGA GGTTGGAGAA AACCAGAGAG CAGGGATGCA AACGCCATTT 
CTTATCATGG ACAGAGACTG TAGGATGCCA ATGGGACTAA GCACTGGTAT CATATCTGAT 
TCACAGATCA AGGCTTCAGA GTTTCTGGGT TACTGGGAGC CCAGATTAGC AAGATTAAAC 
AATGGTGGAT CTTATAATGC TTGGAGTGTA GAAAAACTTG CAGCAGAATT TGCCTCTAAA 
CCTTGGATCC AGGTGGACAT GCAAAAGGAA GTCATAATCA CAGGGATCCA GACCCAAGGT 
GCCAAACACT ACCTGAAGTC CTGCTATACC ACAGAGTTCT ATGTAGCTTA CAGTTCCAAC 
CAGATCAACT GGCAGATCTT CAAAGGGAAC AGCACAAGGA ATGTGATGTA TTTTAATGGC 
AATTCAGATG CCTCTACAAT AAAAGAGAAT CAGTTTGACC CACCTATTGT GGCTAGATAT 
ATTAGGATCT CTCCAACTCG AGCCTATAAC AGACCTACCC TTCGATTGGA ACTGCAAGGT 
TGTGAGGTAA ATGGATGTTC CACACCCCTG GGTATGGAAA ATGGAAAGAT AGAAAACAAG 
CAAATCACAG CTTCTTCGTT TAAGAAATCT TGGTGGGGAG ATTACTGGGA ACCCTTCCGT 
GCCCGTCTGA ATGCCCAGGG ACGTGTGAAT GCCTGGCAAG CCAAGGCAAA CAACAATAAG 
CAGTGGCTAG AAATTGATCT ACTCAAGATC AAGAAGATAA CGGCAATTAT AACACAGGGC 
TGCAAGTCTC TGTCCTCTGA AATGTATGTA AAGAGCTATA CCATCCACTA CAGTGAGCAG 
GGAGTGGAAT GGAAACCATA CAGGCTGAAA TCCTCCATGG TGGACAAGAT TTTTGAAGGA 
AATACTAATA CCAAAGGACA TGTGAAGAAC TTTTTCAACC CCCCAATCAT TTCCAGGTTT 
ATCCGTGTCA TTCCTAAAAC ATGGAATCAA AGTATTGCAC TTCGCCTGGA ACTCTTTGGC 
TGTGATATTT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F5 ( NM_000130.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000121.2 Homo sapiens coagulation factor V (F5), mRNA.

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CloneID	OHu26030
Clone ID Related Accession (Same CDS sequence)	NM_000130.4 , NM_000130.5
Accession Version	NM_000130.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	coagulation factor V preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU279657.1, AK226079.1, M16967.1 and Z99572.1. This sequence is a reference standard in the RefSeqGene project. On Dec 14, 2006 this sequence version replaced NM_000130.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M16967.1, AK226079.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTCCCAG GCTGCCCACG CCTCTGGGTC CTGGTGGTCT TGGGCACCAG CTGGGTAGGC 
TGGGGGAGCC AAGGGACAGA AGCGGCACAG CTAAGGCAGT TCTACGTGGC TGCTCAGGGC 
ATCAGTTGGA GCTACCGACC TGAGCCCACA AACTCAAGTT TGAATCTTTC TGTAACTTCC 
TTTAAGAAAA TTGTCTACAG AGAGTATGAA CCATATTTTA AGAAAGAAAA ACCACAATCT 
ACCATTTCAG GACTTCTTGG GCCTACTTTA TATGCTGAAG TCGGAGACAT CATAAAAGTT 
CACTTTAAAA ATAAGGCAGA TAAGCCCTTG AGCATCCATC CTCAAGGAAT TAGGTACAGT 
AAATTATCAG AAGGTGCTTC TTACCTTGAC CACACATTCC CTGCGGAGAA GATGGACGAC 
GCTGTGGCTC CAGGCCGAGA ATACACCTAT GAATGGAGTA TCAGTGAGGA CAGTGGACCC 
ACCCATGATG ACCCTCCATG CCTCACACAC ATCTATTACT CCCATGAAAA TCTGATCGAG 
GATTTCAACT CGGGGCTGAT TGGGCCCCTG CTTATCTGTA AAAAAGGGAC CCTAACTGAG 
GGTGGGACAC AGAAGACGTT TGACAAGCAA ATCGTGCTAC TATTTGCTGT GTTTGATGAA 
AGCAAGAGCT GGAGCCAGTC ATCATCCCTA ATGTACACAG TCAATGGATA TGTGAATGGG 
ACAATGCCAG ATATAACAGT TTGTGCCCAT GACCACATCA GCTGGCATCT GCTGGGAATG 
AGCTCGGGGC CAGAATTATT CTCCATTCAT TTCAACGGCC AGGTCCTGGA GCAGAACCAT 
CATAAGGTCT CAGCCATCAC CCTTGTCAGT GCTACATCCA CTACCGCAAA TATGACTGTG 
GGCCCAGAGG GAAAGTGGAT CATATCTTCT CTCACCCCAA AACATTTGCA AGCTGGGATG 
CAGGCTTACA TTGACATTAA AAACTGCCCA AAGAAAACCA GGAATCTTAA GAAAATAACT 
CGTGAGCAGA GGCGGCACAT GAAGAGGTGG GAATACTTCA TTGCTGCAGA GGAAGTCATT 
TGGGACTATG CACCTGTAAT ACCAGCGAAT ATGGACAAAA AATACAGGTC TCAGCATTTG 
GATAATTTCT CAAACCAAAT TGGAAAACAT TATAAGAAAG TTATGTACAC ACAGTACGAA 
GATGAGTCCT TCACCAAACA TACAGTGAAT CCCAATATGA AAGAAGATGG GATTTTGGGT 
CCTATTATCA GAGCCCAGGT CAGAGACACA CTCAAAATCG TGTTCAAAAA TATGGCCAGC 
CGCCCCTATA GCATTTACCC TCATGGAGTG ACCTTCTCGC CTTATGAAGA TGAAGTCAAC 
TCTTCTTTCA CCTCAGGCAG GAACAACACC ATGATCAGAG CAGTTCAACC AGGGGAAACC 
TATACTTATA AGTGGAACAT CTTAGAGTTT GATGAACCCA CAGAAAATGA TGCCCAGTGC 
TTAACAAGAC CATACTACAG TGACGTGGAC ATCATGAGAG ACATCGCCTC TGGGCTAATA 
GGACTACTTC TAATCTGTAA GAGCAGATCC CTGGACAGGC GAGGAATACA GAGGGCAGCA 
GACATCGAAC AGCAGGCTGT GTTTGCTGTG TTTGATGAGA ACAAAAGCTG GTACCTTGAG 
GACAACATCA ACAAGTTTTG TGAAAATCCT GATGAGGTGA AACGTGATGA CCCCAAGTTT 
TATGAATCAA ACATCATGAG CACTATCAAT GGCTATGTGC CTGAGAGCAT AACTACTCTT 
GGATTCTGCT TTGATGACAC TGTCCAGTGG CACTTCTGTA GTGTGGGGAC CCAGAATGAA 
ATTTTGACCA TCCACTTCAC TGGGCACTCA TTCATCTATG GAAAGAGGCA TGAGGACACC 
TTGACCCTCT TCCCCATGCG TGGAGAATCT GTGACGGTCA CAATGGATAA TGTTGGAACT 
TGGATGTTAA CTTCCATGAA TTCTAGTCCA AGAAGCAAAA AGCTGAGGCT GAAATTCAGG 
GATGTTAAAT GTATCCCAGA TGATGATGAA GACTCATATG AGATTTTTGA ACCTCCAGAA 
TCTACAGTCA TGGCTACACG GAAAATGCAT GATCGTTTAG AACCTGAAGA TGAAGAGAGT 
GATGCTGACT ATGATTACCA GAACAGACTG GCTGCAGCAT TAGGAATCAG GTCATTCCGA 
AACTCATCAT TGAATCAGGA AGAAGAAGAG TTCAATCTTA CTGCCCTAGC TCTGGAGAAT 
GGCACTGAAT TCGTTTCTTC AAACACAGAT ATAATTGTTG GTTCAAATTA TTCTTCCCCA 
AGTAATATTA GTAAGTTCAC TGTCAATAAC CTTGCAGAAC CTCAGAAAGC CCCTTCTCAC 
CAACAAGCCA CCACAGCTGG TTCCCCACTG AGACACCTCA TTGGCAAGAA CTCAGTTCTC 
AATTCTTCCA CAGCAGAGCA TTCCAGCCCA TATTCTGAAG ACCCTATAGA GGATCCTCTA 
CAGCCAGATG TCACAGGGAT ACGTCTACTT TCACTTGGTG CTGGAGAATT CAAAAGTCAA 
GAACATGCTA AGCATAAGGG ACCCAAGGTA GAAAGAGATC AAGCAGCAAA GCACAGGTTC 
TCCTGGATGA AATTACTAGC ACATAAAGTT GGGAGACACC TAAGCCAAGA CACTGGTTCT 
CCTTCCGGAA TGAGGCCCTG GGAGGACCTT CCTAGCCAAG ACACTGGTTC TCCTTCCAGA 
ATGAGGCCCT GGAAGGACCC TCCTAGTGAT CTGTTACTCT TAAAACAAAG TAACTCATCT 
AAGATTTTGG TTGGGAGATG GCATTTGGCT TCTGAGAAAG GTAGCTATGA AATAATCCAA 
GATACTGATG AAGACACAGC TGTTAACAAT TGGCTGATCA GCCCCCAGAA TGCCTCACGT 
GCTTGGGGAG AAAGCACCCC TCTTGCCAAC AAGCCTGGAA AGCAGAGTGG CCACCCAAAG 
TTTCCTAGAG TTAGACATAA ATCTCTACAA GTAAGACAGG ATGGAGGAAA GAGTAGACTG 
AAGAAAAGCC AGTTTCTCAT TAAGACACGA AAAAAGAAAA AAGAGAAGCA CACACACCAT 
GCTCCTTTAT CTCCGAGGAC CTTTCACCCT CTAAGAAGTG AAGCCTACAA CACATTTTCA 
GAAAGAAGAC TTAAGCATTC GTTGGTGCTT CATAAATCCA ATGAAACATC TCTTCCCACA 
GACCTCAATC AGACATTGCC CTCTATGGAT TTTGGCTGGA TAGCCTCACT TCCTGACCAT 
AATCAGAATT CCTCAAATGA CACTGGTCAG GCAAGCTGTC CTCCAGGTCT TTATCAGACA 
GTGCCCCCAG AGGAACACTA TCAAACATTC CCCATTCAAG ACCCTGATCA AATGCACTCT 
ACTTCAGACC CCAGTCACAG ATCCTCTTCT CCAGAGCTCA GTGAAATGCT TGAGTATGAC 
CGAAGTCACA AGTCCTTCCC CACAGATATA AGTCAAATGT CCCCTTCCTC AGAACATGAA 
GTCTGGCAGA CAGTCATCTC TCCAGACCTC AGCCAGGTGA CCCTCTCTCC AGAACTCAGC 
CAGACAAACC TCTCTCCAGA CCTCAGCCAC ACGACTCTCT CTCCAGAACT CATTCAGAGA 
AACCTTTCCC CAGCCCTCGG TCAGATGCCC ATTTCTCCAG ACCTCAGCCA TACAACCCTT 
TCTCCAGACC TCAGCCATAC AACCCTTTCT TTAGACCTCA GCCAGACAAA CCTCTCTCCA 
GAACTCAGTC AGACAAACCT TTCTCCAGCC CTCGGTCAGA TGCCCCTTTC TCCAGACCTC 
AGCCATACAA CCCTTTCTCT AGACTTCAGC CAGACAAACC TCTCTCCAGA ACTCAGCCAT 
ATGACTCTCT CTCCAGAACT CAGTCAGACA AACCTTTCCC CAGCCCTCGG TCAGATGCCC 
ATTTCTCCAG ACCTCAGCCA TACAACCCTT TCTCTAGACT TCAGCCAGAC AAACCTCTCT 
CCAGAACTCA GTCAAACAAA CCTTTCCCCA GCCCTCGGTC AGATGCCCCT TTCTCCAGAC 
CCCAGCCATA CAACCCTTTC TCTAGACCTC AGCCAGACAA ACCTCTCTCC AGAACTCAGT 
CAGACAAACC TTTCCCCAGA CCTCAGTGAG ATGCCCCTCT TTGCAGATCT CAGTCAAATT 
CCCCTTACCC CAGACCTCGA CCAGATGACA CTTTCTCCAG ACCTTGGTGA GACAGATCTT 
TCCCCAAACT TTGGTCAGAT GTCCCTTTCC CCAGACCTCA GCCAGGTGAC TCTCTCTCCA 
GACATCAGTG ACACCACCCT TCTCCCGGAT CTCAGCCAGA TATCACCTCC TCCAGACCTT 
GATCAGATAT TCTACCCTTC TGAATCTAGT CAGTCATTGC TTCTTCAAGA ATTTAATGAG 
TCTTTTCCTT ATCCAGACCT TGGTCAGATG CCATCTCCTT CATCTCCTAC TCTCAATGAT 
ACTTTTCTAT CAAAGGAATT TAATCCACTG GTTATAGTGG GCCTCAGTAA AGATGGTACA 
GATTACATTG AGATCATTCC AAAGGAAGAG GTCCAGAGCA GTGAAGATGA CTATGCTGAA 
ATTGATTATG TGCCCTATGA TGACCCCTAC AAAACTGATG TTAGGACAAA CATCAACTCC 
TCCAGAGATC CTGACAACAT TGCAGCATGG TACCTCCGCA GCAACAATGG AAACAGAAGA 
AATTATTACA TTGCTGCTGA AGAAATATCC TGGGATTATT CAGAATTTGT ACAAAGGGAA 
ACAGATATTG AAGACTCTGA TGATATTCCA GAAGATACCA CATATAAGAA AGTAGTTTTT 
CGAAAGTACC TCGACAGCAC TTTTACCAAA CGTGATCCTC GAGGGGAGTA TGAAGAGCAT 
CTCGGAATTC TTGGTCCTAT TATCAGAGCT GAAGTGGATG ATGTTATCCA AGTTCGTTTT 
AAAAATTTAG CATCCAGACC GTATTCTCTA CATGCCCATG GACTTTCCTA TGAAAAATCA 
TCAGAGGGAA AGACTTATGA AGATGACTCT CCTGAATGGT TTAAGGAAGA TAATGCTGTT 
CAGCCAAATA GCAGTTATAC CTACGTATGG CATGCCACTG AGCGATCAGG GCCAGAAAGT 
CCTGGCTCTG CCTGTCGGGC TTGGGCCTAC TACTCAGCTG TGAACCCAGA AAAAGATATT 
CACTCAGGCT TGATAGGTCC CCTCCTAATC TGCCAAAAAG GAATACTACA TAAGGACAGC 
AACATGCCTA TGGACATGAG AGAATTTGTC TTACTATTTA TGACCTTTGA TGAAAAGAAG 
AGCTGGTACT ATGAAAAGAA GTCCCGAAGT TCTTGGAGAC TCACATCCTC AGAAATGAAA 
AAATCCCATG AGTTTCACGC CATTAATGGG ATGATCTACA GCTTGCCTGG CCTGAAAATG 
TATGAGCAAG AGTGGGTGAG GTTACACCTG CTGAACATAG GCGGCTCCCA AGACATTCAC 
GTGGTTCACT TTCACGGCCA GACCTTGCTG GAAAATGGCA ATAAACAGCA CCAGTTAGGG 
GTCTGGCCCC TTCTGCCTGG TTCATTTAAA ACTCTTGAAA TGAAGGCATC AAAACCTGGC 
TGGTGGCTCC TAAACACAGA GGTTGGAGAA AACCAGAGAG CAGGGATGCA AACGCCATTT 
CTTATCATGG ACAGAGACTG TAGGATGCCA ATGGGACTAA GCACTGGTAT CATATCTGAT 
TCACAGATCA AGGCTTCAGA GTTTCTGGGT TACTGGGAGC CCAGATTAGC AAGATTAAAC 
AATGGTGGAT CTTATAATGC TTGGAGTGTA GAAAAACTTG CAGCAGAATT TGCCTCTAAA 
CCTTGGATCC AGGTGGACAT GCAAAAGGAA GTCATAATCA CAGGGATCCA GACCCAAGGT 
GCCAAACACT ACCTGAAGTC CTGCTATACC ACAGAGTTCT ATGTAGCTTA CAGTTCCAAC 
CAGATCAACT GGCAGATCTT CAAAGGGAAC AGCACAAGGA ATGTGATGTA TTTTAATGGC 
AATTCAGATG CCTCTACAAT AAAAGAGAAT CAGTTTGACC CACCTATTGT GGCTAGATAT 
ATTAGGATCT CTCCAACTCG AGCCTATAAC AGACCTACCC TTCGATTGGA ACTGCAAGGT 
TGTGAGGTAA ATGGATGTTC CACACCCCTG GGTATGGAAA ATGGAAAGAT AGAAAACAAG 
CAAATCACAG CTTCTTCGTT TAAGAAATCT TGGTGGGGAG ATTACTGGGA ACCCTTCCGT 
GCCCGTCTGA ATGCCCAGGG ACGTGTGAAT GCCTGGCAAG CCAAGGCAAA CAACAATAAG 
CAGTGGCTAG AAATTGATCT ACTCAAGATC AAGAAGATAA CGGCAATTAT AACACAGGGC 
TGCAAGTCTC TGTCCTCTGA AATGTATGTA AAGAGCTATA CCATCCACTA CAGTGAGCAG 
GGAGTGGAAT GGAAACCATA CAGGCTGAAA TCCTCCATGG TGGACAAGAT TTTTGAAGGA 
AATACTAATA CCAAAGGACA TGTGAAGAAC TTTTTCAACC CCCCAATCAT TTCCAGGTTT 
ATCCGTGTCA TTCCTAAAAC ATGGAATCAA AGTATTGCAC TTCGCCTGGA ACTCTTTGGC 
TGTGATATTT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000121.2
CDS	146..6820
Translation	MFPGCPRLWVLVVLGTSWVGWGSQGTEAAQLRQFYVAAQGISWSYRPEPTNSSLNLSVTSFKKIVYREYEPYFKKEKPQSTISGLLGPTLYAEVGDIIKVHFKNKADKPLSIHPQGIRYSKLSEGASYLDHTFPAEKMDDAVAPGREYTYEWSISEDSGPTHDDPPCLTHIYYSHENLIEDFNSGLIGPLLICKKGTLTEGGTQKTFDKQIVLLFAVFDESKSWSQSSSLMYTVNGYVNGTMPDITVCAHDHISWHLLGMSSGPELFSIHFNGQVLEQNHHKVSAITLVSATSTTANMTVGPEGKWIISSLTPKHLQAGMQAYIDIKNCPKKTRNLKKITREQRRHMKRWEYFIAAEEVIWDYAPVIPANMDKKYRSQHLDNFSNQIGKHYKKVMYTQYEDESFTKHTVNPNMKEDGILGPIIRAQVRDTLKIVFKNMASRPYSIYPHGVTFSPYEDEVNSSFTSGRNNTMIRAVQPGETYTYKWNILEFDEPTENDAQCLTRPYYSDVDIMRDIASGLIGLLLICKSRSLDRRGIQRAADIEQQAVFAVFDENKSWYLEDNINKFCENPDEVKRDDPKFYESNIMSTINGYVPESITTLGFCFDDTVQWHFCSVGTQNEILTIHFTGHSFIYGKRHEDTLTLFPMRGESVTVTMDNVGTWMLTSMNSSPRSKKLRLKFRDVKCIPDDDEDSYEIFEPPESTVMATRKMHDRLEPEDEESDADYDYQNRLAAALGIRSFRNSSLNQEEEEFNLTALALENGTEFVSSNTDIIVGSNYSSPSNISKFTVNNLAEPQKAPSHQQATTAGSPLRHLIGKNSVLNSSTAEHSSPYSEDPIEDPLQPDVTGIRLLSLGAGEFKSQEHAKHKGPKVERDQAAKHRFSWMKLLAHKVGRHLSQDTGSPSGMRPWEDLPSQDTGSPSRMRPWKDPPSDLLLLKQSNSSKILVGRWHLASEKGSYEIIQDTDEDTAVNNWLISPQNASRAWGESTPLANKPGKQSGHPKFPRVRHKSLQVRQDGGKSRLKKSQFLIKTRKKKKEKHTHHAPLSPRTFHPLRSEAYNTFSERRLKHSLVLHKSNETSLPTDLNQTLPSMDFGWIASLPDHNQNSSNDTGQASCPPGLYQTVPPEEHYQTFPIQDPDQMHSTSDPSHRSSSPELSEMLEYDRSHKSFPTDISQMSPSSEHEVWQTVISPDLSQVTLSPELSQTNLSPDLSHTTLSPELIQRNLSPALGQMPISPDLSHTTLSPDLSHTTLSLDLSQTNLSPELSQTNLSPALGQMPLSPDLSHTTLSLDFSQTNLSPELSHMTLSPELSQTNLSPALGQMPISPDLSHTTLSLDFSQTNLSPELSQTNLSPALGQMPLSPDPSHTTLSLDLSQTNLSPELSQTNLSPDLSEMPLFADLSQIPLTPDLDQMTLSPDLGETDLSPNFGQMSLSPDLSQVTLSPDISDTTLLPDLSQISPPPDLDQIFYPSESSQSLLLQEFNESFPYPDLGQMPSPSSPTLNDTFLSKEFNPLVIVGLSKDGTDYIEIIPKEEVQSSEDDYAEIDYVPYDDPYKTDVRTNINSSRDPDNIAAWYLRSNNGNRRNYYIAAEEISWDYSEFVQRETDIEDSDDIPEDTTYKKVVFRKYLDSTFTKRDPRGEYEEHLGILGPIIRAEVDDVIQVRFKNLASRPYSLHAHGLSYEKSSEGKTYEDDSPEWFKEDNAVQPNSSYTYVWHATERSGPESPGSACRAWAYYSAVNPEKDIHSGLIGPLLICQKGILHKDSNMPMDMREFVLLFMTFDEKKSWYYEKKSRSSWRLTSSEMKKSHEFHAINGMIYSLPGLKMYEQEWVRLHLLNIGGSQDIHVVHFHGQTLLENGNKQHQLGVWPLLPGSFKTLEMKASKPGWWLLNTEVGENQRAGMQTPFLIMDRDCRMPMGLSTGIISDSQIKASEFLGYWEPRLARLNNGGSYNAWSVEKLAAEFASKPWIQVDMQKEVIITGIQTQGAKHYLKSCYTTEFYVAYSSNQINWQIFKGNSTRNVMYFNGNSDASTIKENQFDPPIVARYIRISPTRAYNRPTLRLELQGCEVNGCSTPLGMENGKIENKQITASSFKKSWWGDYWEPFRARLNAQGRVNAWQAKANNNKQWLEIDLLKIKKITAIITQGCKSLSSEMYVKSYTIHYSEQGVEWKPYRLKSSMVDKIFEGNTNTKGHVKNFFNPPIISRFIRVIPKTWNQSIALRLELFGCDIY

Target ORF information:

RefSeq Version	NM_000130.4
Organism	Homo sapiens(human)
Definition	Homo sapiens coagulation factor V (F5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000130.4

ORF Insert Sequence:

ATGTTCCCAG GCTGCCCACG CCTCTGGGTC CTGGTGGTCT TGGGCACCAG CTGGGTAGGC 
TGGGGGAGCC AAGGGACAGA AGCGGCACAG CTAAGGCAGT TCTACGTGGC TGCTCAGGGC 
ATCAGTTGGA GCTACCGACC TGAGCCCACA AACTCAAGTT TGAATCTTTC TGTAACTTCC 
TTTAAGAAAA TTGTCTACAG AGAGTATGAA CCATATTTTA AGAAAGAAAA ACCACAATCT 
ACCATTTCAG GACTTCTTGG GCCTACTTTA TATGCTGAAG TCGGAGACAT CATAAAAGTT 
CACTTTAAAA ATAAGGCAGA TAAGCCCTTG AGCATCCATC CTCAAGGAAT TAGGTACAGT 
AAATTATCAG AAGGTGCTTC TTACCTTGAC CACACATTCC CTGCGGAGAA GATGGACGAC 
GCTGTGGCTC CAGGCCGAGA ATACACCTAT GAATGGAGTA TCAGTGAGGA CAGTGGACCC 
ACCCATGATG ACCCTCCATG CCTCACACAC ATCTATTACT CCCATGAAAA TCTGATCGAG 
GATTTCAACT CGGGGCTGAT TGGGCCCCTG CTTATCTGTA AAAAAGGGAC CCTAACTGAG 
GGTGGGACAC AGAAGACGTT TGACAAGCAA ATCGTGCTAC TATTTGCTGT GTTTGATGAA 
AGCAAGAGCT GGAGCCAGTC ATCATCCCTA ATGTACACAG TCAATGGATA TGTGAATGGG 
ACAATGCCAG ATATAACAGT TTGTGCCCAT GACCACATCA GCTGGCATCT GCTGGGAATG 
AGCTCGGGGC CAGAATTATT CTCCATTCAT TTCAACGGCC AGGTCCTGGA GCAGAACCAT 
CATAAGGTCT CAGCCATCAC CCTTGTCAGT GCTACATCCA CTACCGCAAA TATGACTGTG 
GGCCCAGAGG GAAAGTGGAT CATATCTTCT CTCACCCCAA AACATTTGCA AGCTGGGATG 
CAGGCTTACA TTGACATTAA AAACTGCCCA AAGAAAACCA GGAATCTTAA GAAAATAACT 
CGTGAGCAGA GGCGGCACAT GAAGAGGTGG GAATACTTCA TTGCTGCAGA GGAAGTCATT 
TGGGACTATG CACCTGTAAT ACCAGCGAAT ATGGACAAAA AATACAGGTC TCAGCATTTG 
GATAATTTCT CAAACCAAAT TGGAAAACAT TATAAGAAAG TTATGTACAC ACAGTACGAA 
GATGAGTCCT TCACCAAACA TACAGTGAAT CCCAATATGA AAGAAGATGG GATTTTGGGT 
CCTATTATCA GAGCCCAGGT CAGAGACACA CTCAAAATCG TGTTCAAAAA TATGGCCAGC 
CGCCCCTATA GCATTTACCC TCATGGAGTG ACCTTCTCGC CTTATGAAGA TGAAGTCAAC 
TCTTCTTTCA CCTCAGGCAG GAACAACACC ATGATCAGAG CAGTTCAACC AGGGGAAACC 
TATACTTATA AGTGGAACAT CTTAGAGTTT GATGAACCCA CAGAAAATGA TGCCCAGTGC 
TTAACAAGAC CATACTACAG TGACGTGGAC ATCATGAGAG ACATCGCCTC TGGGCTAATA 
GGACTACTTC TAATCTGTAA GAGCAGATCC CTGGACAGGC GAGGAATACA GAGGGCAGCA 
GACATCGAAC AGCAGGCTGT GTTTGCTGTG TTTGATGAGA ACAAAAGCTG GTACCTTGAG 
GACAACATCA ACAAGTTTTG TGAAAATCCT GATGAGGTGA AACGTGATGA CCCCAAGTTT 
TATGAATCAA ACATCATGAG CACTATCAAT GGCTATGTGC CTGAGAGCAT AACTACTCTT 
GGATTCTGCT TTGATGACAC TGTCCAGTGG CACTTCTGTA GTGTGGGGAC CCAGAATGAA 
ATTTTGACCA TCCACTTCAC TGGGCACTCA TTCATCTATG GAAAGAGGCA TGAGGACACC 
TTGACCCTCT TCCCCATGCG TGGAGAATCT GTGACGGTCA CAATGGATAA TGTTGGAACT 
TGGATGTTAA CTTCCATGAA TTCTAGTCCA AGAAGCAAAA AGCTGAGGCT GAAATTCAGG 
GATGTTAAAT GTATCCCAGA TGATGATGAA GACTCATATG AGATTTTTGA ACCTCCAGAA 
TCTACAGTCA TGGCTACACG GAAAATGCAT GATCGTTTAG AACCTGAAGA TGAAGAGAGT 
GATGCTGACT ATGATTACCA GAACAGACTG GCTGCAGCAT TAGGAATCAG GTCATTCCGA 
AACTCATCAT TGAATCAGGA AGAAGAAGAG TTCAATCTTA CTGCCCTAGC TCTGGAGAAT 
GGCACTGAAT TCGTTTCTTC AAACACAGAT ATAATTGTTG GTTCAAATTA TTCTTCCCCA 
AGTAATATTA GTAAGTTCAC TGTCAATAAC CTTGCAGAAC CTCAGAAAGC CCCTTCTCAC 
CAACAAGCCA CCACAGCTGG TTCCCCACTG AGACACCTCA TTGGCAAGAA CTCAGTTCTC 
AATTCTTCCA CAGCAGAGCA TTCCAGCCCA TATTCTGAAG ACCCTATAGA GGATCCTCTA 
CAGCCAGATG TCACAGGGAT ACGTCTACTT TCACTTGGTG CTGGAGAATT CAAAAGTCAA 
GAACATGCTA AGCATAAGGG ACCCAAGGTA GAAAGAGATC AAGCAGCAAA GCACAGGTTC 
TCCTGGATGA AATTACTAGC ACATAAAGTT GGGAGACACC TAAGCCAAGA CACTGGTTCT 
CCTTCCGGAA TGAGGCCCTG GGAGGACCTT CCTAGCCAAG ACACTGGTTC TCCTTCCAGA 
ATGAGGCCCT GGAAGGACCC TCCTAGTGAT CTGTTACTCT TAAAACAAAG TAACTCATCT 
AAGATTTTGG TTGGGAGATG GCATTTGGCT TCTGAGAAAG GTAGCTATGA AATAATCCAA 
GATACTGATG AAGACACAGC TGTTAACAAT TGGCTGATCA GCCCCCAGAA TGCCTCACGT 
GCTTGGGGAG AAAGCACCCC TCTTGCCAAC AAGCCTGGAA AGCAGAGTGG CCACCCAAAG 
TTTCCTAGAG TTAGACATAA ATCTCTACAA GTAAGACAGG ATGGAGGAAA GAGTAGACTG 
AAGAAAAGCC AGTTTCTCAT TAAGACACGA AAAAAGAAAA AAGAGAAGCA CACACACCAT 
GCTCCTTTAT CTCCGAGGAC CTTTCACCCT CTAAGAAGTG AAGCCTACAA CACATTTTCA 
GAAAGAAGAC TTAAGCATTC GTTGGTGCTT CATAAATCCA ATGAAACATC TCTTCCCACA 
GACCTCAATC AGACATTGCC CTCTATGGAT TTTGGCTGGA TAGCCTCACT TCCTGACCAT 
AATCAGAATT CCTCAAATGA CACTGGTCAG GCAAGCTGTC CTCCAGGTCT TTATCAGACA 
GTGCCCCCAG AGGAACACTA TCAAACATTC CCCATTCAAG ACCCTGATCA AATGCACTCT 
ACTTCAGACC CCAGTCACAG ATCCTCTTCT CCAGAGCTCA GTGAAATGCT TGAGTATGAC 
CGAAGTCACA AGTCCTTCCC CACAGATATA AGTCAAATGT CCCCTTCCTC AGAACATGAA 
GTCTGGCAGA CAGTCATCTC TCCAGACCTC AGCCAGGTGA CCCTCTCTCC AGAACTCAGC 
CAGACAAACC TCTCTCCAGA CCTCAGCCAC ACGACTCTCT CTCCAGAACT CATTCAGAGA 
AACCTTTCCC CAGCCCTCGG TCAGATGCCC ATTTCTCCAG ACCTCAGCCA TACAACCCTT 
TCTCCAGACC TCAGCCATAC AACCCTTTCT TTAGACCTCA GCCAGACAAA CCTCTCTCCA 
GAACTCAGTC AGACAAACCT TTCTCCAGCC CTCGGTCAGA TGCCCCTTTC TCCAGACCTC 
AGCCATACAA CCCTTTCTCT AGACTTCAGC CAGACAAACC TCTCTCCAGA ACTCAGCCAT 
ATGACTCTCT CTCCAGAACT CAGTCAGACA AACCTTTCCC CAGCCCTCGG TCAGATGCCC 
ATTTCTCCAG ACCTCAGCCA TACAACCCTT TCTCTAGACT TCAGCCAGAC AAACCTCTCT 
CCAGAACTCA GTCAAACAAA CCTTTCCCCA GCCCTCGGTC AGATGCCCCT TTCTCCAGAC 
CCCAGCCATA CAACCCTTTC TCTAGACCTC AGCCAGACAA ACCTCTCTCC AGAACTCAGT 
CAGACAAACC TTTCCCCAGA CCTCAGTGAG ATGCCCCTCT TTGCAGATCT CAGTCAAATT 
CCCCTTACCC CAGACCTCGA CCAGATGACA CTTTCTCCAG ACCTTGGTGA GACAGATCTT 
TCCCCAAACT TTGGTCAGAT GTCCCTTTCC CCAGACCTCA GCCAGGTGAC TCTCTCTCCA 
GACATCAGTG ACACCACCCT TCTCCCGGAT CTCAGCCAGA TATCACCTCC TCCAGACCTT 
GATCAGATAT TCTACCCTTC TGAATCTAGT CAGTCATTGC TTCTTCAAGA ATTTAATGAG 
TCTTTTCCTT ATCCAGACCT TGGTCAGATG CCATCTCCTT CATCTCCTAC TCTCAATGAT 
ACTTTTCTAT CAAAGGAATT TAATCCACTG GTTATAGTGG GCCTCAGTAA AGATGGTACA 
GATTACATTG AGATCATTCC AAAGGAAGAG GTCCAGAGCA GTGAAGATGA CTATGCTGAA 
ATTGATTATG TGCCCTATGA TGACCCCTAC AAAACTGATG TTAGGACAAA CATCAACTCC 
TCCAGAGATC CTGACAACAT TGCAGCATGG TACCTCCGCA GCAACAATGG AAACAGAAGA 
AATTATTACA TTGCTGCTGA AGAAATATCC TGGGATTATT CAGAATTTGT ACAAAGGGAA 
ACAGATATTG AAGACTCTGA TGATATTCCA GAAGATACCA CATATAAGAA AGTAGTTTTT 
CGAAAGTACC TCGACAGCAC TTTTACCAAA CGTGATCCTC GAGGGGAGTA TGAAGAGCAT 
CTCGGAATTC TTGGTCCTAT TATCAGAGCT GAAGTGGATG ATGTTATCCA AGTTCGTTTT 
AAAAATTTAG CATCCAGACC GTATTCTCTA CATGCCCATG GACTTTCCTA TGAAAAATCA 
TCAGAGGGAA AGACTTATGA AGATGACTCT CCTGAATGGT TTAAGGAAGA TAATGCTGTT 
CAGCCAAATA GCAGTTATAC CTACGTATGG CATGCCACTG AGCGATCAGG GCCAGAAAGT 
CCTGGCTCTG CCTGTCGGGC TTGGGCCTAC TACTCAGCTG TGAACCCAGA AAAAGATATT 
CACTCAGGCT TGATAGGTCC CCTCCTAATC TGCCAAAAAG GAATACTACA TAAGGACAGC 
AACATGCCTA TGGACATGAG AGAATTTGTC TTACTATTTA TGACCTTTGA TGAAAAGAAG 
AGCTGGTACT ATGAAAAGAA GTCCCGAAGT TCTTGGAGAC TCACATCCTC AGAAATGAAA 
AAATCCCATG AGTTTCACGC CATTAATGGG ATGATCTACA GCTTGCCTGG CCTGAAAATG 
TATGAGCAAG AGTGGGTGAG GTTACACCTG CTGAACATAG GCGGCTCCCA AGACATTCAC 
GTGGTTCACT TTCACGGCCA GACCTTGCTG GAAAATGGCA ATAAACAGCA CCAGTTAGGG 
GTCTGGCCCC TTCTGCCTGG TTCATTTAAA ACTCTTGAAA TGAAGGCATC AAAACCTGGC 
TGGTGGCTCC TAAACACAGA GGTTGGAGAA AACCAGAGAG CAGGGATGCA AACGCCATTT 
CTTATCATGG ACAGAGACTG TAGGATGCCA ATGGGACTAA GCACTGGTAT CATATCTGAT 
TCACAGATCA AGGCTTCAGA GTTTCTGGGT TACTGGGAGC CCAGATTAGC AAGATTAAAC 
AATGGTGGAT CTTATAATGC TTGGAGTGTA GAAAAACTTG CAGCAGAATT TGCCTCTAAA 
CCTTGGATCC AGGTGGACAT GCAAAAGGAA GTCATAATCA CAGGGATCCA GACCCAAGGT 
GCCAAACACT ACCTGAAGTC CTGCTATACC ACAGAGTTCT ATGTAGCTTA CAGTTCCAAC 
CAGATCAACT GGCAGATCTT CAAAGGGAAC AGCACAAGGA ATGTGATGTA TTTTAATGGC 
AATTCAGATG CCTCTACAAT AAAAGAGAAT CAGTTTGACC CACCTATTGT GGCTAGATAT 
ATTAGGATCT CTCCAACTCG AGCCTATAAC AGACCTACCC TTCGATTGGA ACTGCAAGGT 
TGTGAGGTAA ATGGATGTTC CACACCCCTG GGTATGGAAA ATGGAAAGAT AGAAAACAAG 
CAAATCACAG CTTCTTCGTT TAAGAAATCT TGGTGGGGAG ATTACTGGGA ACCCTTCCGT 
GCCCGTCTGA ATGCCCAGGG ACGTGTGAAT GCCTGGCAAG CCAAGGCAAA CAACAATAAG 
CAGTGGCTAG AAATTGATCT ACTCAAGATC AAGAAGATAA CGGCAATTAT AACACAGGGC 
TGCAAGTCTC TGTCCTCTGA AATGTATGTA AAGAGCTATA CCATCCACTA CAGTGAGCAG 
GGAGTGGAAT GGAAACCATA CAGGCTGAAA TCCTCCATGG TGGACAAGAT TTTTGAAGGA 
AATACTAATA CCAAAGGACA TGTGAAGAAC TTTTTCAACC CCCCAATCAT TTCCAGGTTT 
ATCCGTGTCA TTCCTAAAAC ATGGAATCAA AGTATTGCAC TTCGCCTGGA ACTCTTTGGC 
TGTGATATTT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F5 ( XM_017000660.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856149.1 Homo sapiens coagulation factor V (F5), transcript variant X1, mRNA.

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CloneID	OHu83340
Clone ID Related Accession (Same CDS sequence)	XM_017000660.1 , XM_017000660.2
Accession Version	XM_017000660.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	coagulation factor V isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGACG CTGTGGCTCC AGGCCGAGAA TACACCTATG AATGGAGTAT CAGTGAGGAC 
AGTGGACCCA CCCATGATGA CCCTCCATGC CTCACACACA TCTATTACTC CCATGAAAAT 
CTGATCGAGG ATTTCAACTC GGGGCTGATT GGGCCCCTGC TTATCTGTAA AAAAGGGACC 
CTAACTGAGG GTGGGACACA GAAGACGTTT GACAAGCAAA TCGTGCTACT ATTTGCTGTG 
TTTGATGAAA GCAAGAGCTG GAGCCAGTCA TCATCCCTAA TGTACACAGT CAATGGATAT 
GTGAATGGGA CAATGCCAGA TATAACAGTT TGTGCCCATG ACCACATCAG CTGGCATCTG 
CTGGGAATGA GCTCGGGGCC AGAATTATTC TCCATTCATT TCAACGGCCA GGTCCTGGAG 
CAGAACCATC ATAAGGTCTC AGCCATCACC CTTGTCAGTG CTACATCCAC TACCGCAAAT 
ATGACTGTGG GCCCAGAGGG AAAGTGGATC ATATCTTCTC TCACCCCAAA ACATTTGCAA 
GCTGGGATGC AGGCTTACAT TGACATTAAA AACTGCCCAA AGAAAACCAG GAATCTTAAG 
AAAATAACTC GTGAGCAGAG GCGGCACATG AAGAGGTGGG AATACTTCAT TGCTGCAGAG 
GAAGTCATTT GGGACTATGC ACCTGTAATA CCAGCGAATA TGGACAAAAA ATACAGGTCT 
CAGCATTTGG ATAATTTCTC AAACCAAATT GGAAAACATT ATAAGAAAGT TATGTACACA 
CAGTACGAAG ATGAGTCCTT CACCAAACAT ACAGTGAATC CCAATATGAA AGAAGATGGG 
ATTTTGGGTC CTATTATCAG AGCCCAGGTC AGAGACACAC TCAAAATCGT GTTCAAAAAT 
ATGGCCAGCC GCCCCTATAG CATTTACCCT CATGGAGTGA CCTTCTCGCC TTATGAAGAT 
GAAGTCAACT CTTCTTTCAC CTCAGGCAGG AACAACACCA TGATCAGAGC AGTTCAACCA 
GGGGAAACCT ATACTTATAA GTGGAACATC TTAGAGTTTG ATGAACCCAC AGAAAATGAT 
GCCCAGTGCT TAACAAGACC ATACTACAGT GACGTGGACA TCATGAGAGA CATCGCCTCT 
GGGCTAATAG GACTACTTCT AATCTGTAAG AGCAGATCCC TGGACAGGCG AGGAATACAG 
AGGGCAGCAG ACATCGAACA GCAGGCTGTG TTTGCTGTGT TTGATGAGAA CAAAAGCTGG 
TACCTTGAGG ACAACATCAA CAAGTTTTGT GAAAATCCTG ATGAGGTGAA ACGTGATGAC 
CCCAAGTTTT ATGAATCAAA CATCATGAGC ACTATCAATG GCTATGTGCC TGAGAGCATA 
ACTACTCTTG GATTCTGCTT TGATGACACT GTCCAGTGGC ACTTCTGTAG TGTGGGGACC 
CAGAATGAAA TTTTGACCAT CCACTTCACT GGGCACTCAT TCATCTATGG AAAGAGGCAT 
GAGGACACCT TGACCCTCTT CCCCATGCGT GGAGAATCTG TGACGGTCAC AATGGATAAT 
GTTGGAACTT GGATGTTAAC TTCCATGAAT TCTAGTCCAA GAAGCAAAAA GCTGAGGCTG 
AAATTCAGGG ATGTTAAATG TATCCCAGAT GATGATGAAG ACTCATATGA GATTTTTGAA 
CCTCCAGAAT CTACAGTCAT GGCTACACGG AAAATGCATG ATCGTTTAGA ACCTGAAGAT 
GAAGAGAGTG ATGCTGACTA TGATTACCAG AACAGACTGG CTGCAGCATT AGGAATCAGG 
TCATTCCGAA ACTCATCATT GAATCAGGAA GAAGAAGAGT TCAATCTTAC TGCCCTAGCT 
CTGGAGAATG GCACTGAATT CGTTTCTTCA AACACAGATA TAATTGTTGG TTCAAATTAT 
TCTTCCCCAA GTAATATTAG TAAGTTCACT GTCAATAACC TTGCAGAACC TCAGAAAGCC 
CCTTCTCACC AACAAGCCAC CACAGCTGGT TCCCCACTGA GACACCTCAT TGGCAAGAAC 
TCAGTTCTCA ATTCTTCCAC AGCAGAGCAT TCCAGCCCAT ATTCTGAAGA CCCTATAGAG 
GATCCTCTAC AGCCAGATGT CACAGGGATA CGTCTACTTT CACTTGGTGC TGGAGAATTC 
AAAAGTCAAG AACATGCTAA GCATAAGGGA CCCAAGGTAG AAAGAGATCA AGCAGCAAAG 
CACAGGTTCT CCTGGATGAA ATTACTAGCA CATAAAGTTG GGAGACACCT AAGCCAAGAC 
ACTGGTTCTC CTTCCGGAAT GAGGCCCTGG GAGGACCTTC CTAGCCAAGA CACTGGTTCT 
CCTTCCAGAA TGAGGCCCTG GAAGGACCCT CCTAGTGATC TGTTACTCTT AAAACAAAGT 
AACTCATCTA AGATTTTGGT TGGGAGATGG CATTTGGCTT CTGAGAAAGG TAGCTATGAA 
ATAATCCAAG ATACTGATGA AGACACAGCT GTTAACAATT GGCTGATCAG CCCCCAGAAT 
GCCTCACGTG CTTGGGGAGA AAGCACCCCT CTTGCCAACA AGCCTGGAAA GCAGAGTGGC 
CACCCAAAGT TTCCTAGAGT TAGACATAAA TCTCTACAAG TAAGACAGGA TGGAGGAAAG 
AGTAGACTGA AGAAAAGCCA GTTTCTCATT AAGACACGAA AAAAGAAAAA AGAGAAGCAC 
ACACACCATG CTCCTTTATC TCCGAGGACC TTTCACCCTC TAAGAAGTGA AGCCTACAAC 
ACATTTTCAG AAAGAAGACT TAAGCATTCG TTGGTGCTTC ATAAATCCAA TGAAACATCT 
CTTCCCACAG ACCTCAATCA GACATTGCCC TCTATGGATT TTGGCTGGAT AGCCTCACTT 
CCTGACCATA ATCAGAATTC CTCAAATGAC ACTGGTCAGG CAAGCTGTCC TCCAGGTCTT 
TATCAGACAG TGCCCCCAGA GGAACACTAT CAAACATTCC CCATTCAAGA CCCTGATCAA 
ATGCACTCTA CTTCAGACCC CAGTCACAGA TCCTCTTCTC CAGAGCTCAG TGAAATGCTT 
GAGTATGACC GAAGTCACAA GTCCTTCCCC ACAGATATAA GTCAAATGTC CCCTTCCTCA 
GAACATGAAG TCTGGCAGAC AGTCATCTCT CCAGACCTCA GCCAGGTGAC CCTCTCTCCA 
GAACTCAGCC AGACAAACCT CTCTCCAGAC CTCAGCCACA CGACTCTCTC TCCAGAACTC 
ATTCAGAGAA ACCTTTCCCC AGCCCTCGGT CAGATGCCCA TTTCTCCAGA CCTCAGCCAT 
ACAACCCTTT CTCCAGACCT CAGCCATACA ACCCTTTCTT TAGACCTCAG CCAGACAAAC 
CTCTCTCCAG AACTCAGTCA GACAAACCTT TCTCCAGCCC TCGGTCAGAT GCCCCTTTCT 
CCAGACCTCA GCCATACAAC CCTTTCTCTA GACTTCAGCC AGACAAACCT CTCTCCAGAA 
CTCAGCCATA TGACTCTCTC TCCAGAACTC AGTCAGACAA ACCTTTCCCC AGCCCTCGGT 
CAGATGCCCA TTTCTCCAGA CCTCAGCCAT ACAACCCTTT CTCTAGACTT CAGCCAGACA 
AACCTCTCTC CAGAACTCAG TCAAACAAAC CTTTCCCCAG CCCTCGGTCA GATGCCCCTT 
TCTCCAGACC CCAGCCATAC AACCCTTTCT CTAGACCTCA GCCAGACAAA CCTCTCTCCA 
GAACTCAGTC AGACAAACCT TTCCCCAGAC CTCAGTGAGA TGCCCCTCTT TGCAGATCTC 
AGTCAAATTC CCCTTACCCC AGACCTCGAC CAGATGACAC TTTCTCCAGA CCTTGGTGAG 
ACAGATCTTT CCCCAAACTT TGGTCAGATG TCCCTTTCCC CAGACCTCAG CCAGGTGACT 
CTCTCTCCAG ACATCAGTGA CACCACCCTT CTCCCGGATC TCAGCCAGAT ATCACCTCCT 
CCAGACCTTG ATCAGATATT CTACCCTTCT GAATCTAGTC AGTCATTGCT TCTTCAAGAA 
TTTAATGAGT CTTTTCCTTA TCCAGACCTT GGTCAGATGC CATCTCCTTC ATCTCCTACT 
CTCAATGATA CTTTTCTATC AAAGGAATTT AATCCACTGG TTATAGTGGG CCTCAGTAAA 
GATGGTACAG ATTACATTGA GATCATTCCA AAGGAAGAGG TCCAGAGCAG TGAAGATGAC 
TATGCTGAAA TTGATTATGT GCCCTATGAT GACCCCTACA AAACTGATGT TAGGACAAAC 
ATCAACTCCT CCAGAGATCC TGACAACATT GCAGCATGGT ACCTCCGCAG CAACAATGGA 
AACAGAAGAA ATTATTACAT TGCTGCTGAA GAAATATCCT GGGATTATTC AGAATTTGTA 
CAAAGGGAAA CAGATATTGA AGACTCTGAT GATATTCCAG AAGATACCAC ATATAAGAAA 
GTAGTTTTTC GAAAGTACCT CGACAGCACT TTTACCAAAC GTGATCCTCG AGGGGAGTAT 
GAAGAGCATC TCGGAATTCT TGGTCCTATT ATCAGAGCTG AAGTGGATGA TGTTATCCAA 
GTTCGTTTTA AAAATTTAGC ATCCAGACCG TATTCTCTAC ATGCCCATGG ACTTTCCTAT 
GAAAAATCAT CAGAGGGAAA GACTTATGAA GATGACTCTC CTGAATGGTT TAAGGAAGAT 
AATGCTGTTC AGCCAAATAG CAGTTATACC TACGTATGGC ATGCCACTGA GCGATCAGGG 
CCAGAAAGTC CTGGCTCTGC CTGTCGGGCT TGGGCCTACT ACTCAGCTGT GAACCCAGAA 
AAAGATATTC ACTCAGGCTT GATAGGTCCC CTCCTAATCT GCCAAAAAGG AATACTACAT 
AAGGACAGCA ACATGCCTAT GGACATGAGA GAATTTGTCT TACTATTTAT GACCTTTGAT 
GAAAAGAAGA GCTGGTACTA TGAAAAGAAG TCCCGAAGTT CTTGGAGACT CACATCCTCA 
GAAATGAAAA AATCCCATGA GTTTCACGCC ATTAATGGGA TGATCTACAG CTTGCCTGGC 
CTGAAAATGT ATGAGCAAGA GTGGGTGAGG TTACACCTGC TGAACATAGG CGGCTCCCAA 
GACATTCACG TGGTTCACTT TCACGGCCAG ACCTTGCTGG AAAATGGCAA TAAACAGCAC 
CAGTTAGGGG TCTGGCCCCT TCTGCCTGGT TCATTTAAAA CTCTTGAAAT GAAGGCATCA 
AAACCTGGCT GGTGGCTCCT AAACACAGAG GTTGGAGAAA ACCAGAGAGC AGGGATGCAA 
ACGCCATTTC TTATCATGGA CAGAGACTGT AGGATGCCAA TGGGACTAAG CACTGGTATC 
ATATCTGATT CACAGATCAA GGCTTCAGAG TTTCTGGGTT ACTGGGAGCC CAGATTAGCA 
AGATTAAACA ATGGTGGATC TTATAATGCT TGGAGTGTAG AAAAACTTGC AGCAGAATTT 
GCCTCTAAAC CTTGGATCCA GGTGGACATG CAAAAGGAAG TCATAATCAC AGGGATCCAG 
ACCCAAGGTG CCAAACACTA CCTGAAGTCC TGCTATACCA CAGAGTTCTA TGTAGCTTAC 
AGTTCCAACC AGATCAACTG GCAGATCTTC AAAGGGAACA GCACAAGGAA TGTGATGTAT 
TTTAATGGCA ATTCAGATGC CTCTACAATA AAAGAGAATC AGTTTGACCC ACCTATTGTG 
GCTAGATATA TTAGGATCTC TCCAACTCGA GCCTATAACA GACCTACCCT TCGATTGGAA 
CTGCAAGGTT GTGAGGTAAA TGGATGTTCC ACACCCCTGG GTATGGAAAA TGGAAAGATA 
GAAAACAAGC AAATCACAGC TTCTTCGTTT AAGAAATCTT GGTGGGGAGA TTACTGGGAA 
CCCTTCCGTG CCCGTCTGAA TGCCCAGGGA CGTGTGAATG CCTGGCAAGC CAAGGCAAAC 
AACAATAAGC AGTGGCTAGA AATTGATCTA CTCAAGATCA AGAAGATAAC GGCAATTATA 
ACACAGGGCT GCAAGTCTCT GTCCTCTGAA ATGTATGTAA AGAGCTATAC CATCCACTAC 
AGTGAGCAGG GAGTGGAATG GAAACCATAC AGGCTGAAAT CCTCCATGGT GGACAAGATT 
TTTGAAGGAA ATACTAATAC CAAAGGACAT GTGAAGAACT TTTTCAACCC CCCAATCATT 
TCCAGGTTTA TCCGTGTCAT TCCTAAAACA TGGAATCAAA GTATTGCACT TCGCCTGGAA 
CTCTTTGGCT GTGATATTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856149.1
CDS	566..6829
Translation	MDDAVAPGREYTYEWSISEDSGPTHDDPPCLTHIYYSHENLIEDFNSGLIGPLLICKKGTLTEGGTQKTFDKQIVLLFAVFDESKSWSQSSSLMYTVNGYVNGTMPDITVCAHDHISWHLLGMSSGPELFSIHFNGQVLEQNHHKVSAITLVSATSTTANMTVGPEGKWIISSLTPKHLQAGMQAYIDIKNCPKKTRNLKKITREQRRHMKRWEYFIAAEEVIWDYAPVIPANMDKKYRSQHLDNFSNQIGKHYKKVMYTQYEDESFTKHTVNPNMKEDGILGPIIRAQVRDTLKIVFKNMASRPYSIYPHGVTFSPYEDEVNSSFTSGRNNTMIRAVQPGETYTYKWNILEFDEPTENDAQCLTRPYYSDVDIMRDIASGLIGLLLICKSRSLDRRGIQRAADIEQQAVFAVFDENKSWYLEDNINKFCENPDEVKRDDPKFYESNIMSTINGYVPESITTLGFCFDDTVQWHFCSVGTQNEILTIHFTGHSFIYGKRHEDTLTLFPMRGESVTVTMDNVGTWMLTSMNSSPRSKKLRLKFRDVKCIPDDDEDSYEIFEPPESTVMATRKMHDRLEPEDEESDADYDYQNRLAAALGIRSFRNSSLNQEEEEFNLTALALENGTEFVSSNTDIIVGSNYSSPSNISKFTVNNLAEPQKAPSHQQATTAGSPLRHLIGKNSVLNSSTAEHSSPYSEDPIEDPLQPDVTGIRLLSLGAGEFKSQEHAKHKGPKVERDQAAKHRFSWMKLLAHKVGRHLSQDTGSPSGMRPWEDLPSQDTGSPSRMRPWKDPPSDLLLLKQSNSSKILVGRWHLASEKGSYEIIQDTDEDTAVNNWLISPQNASRAWGESTPLANKPGKQSGHPKFPRVRHKSLQVRQDGGKSRLKKSQFLIKTRKKKKEKHTHHAPLSPRTFHPLRSEAYNTFSERRLKHSLVLHKSNETSLPTDLNQTLPSMDFGWIASLPDHNQNSSNDTGQASCPPGLYQTVPPEEHYQTFPIQDPDQMHSTSDPSHRSSSPELSEMLEYDRSHKSFPTDISQMSPSSEHEVWQTVISPDLSQVTLSPELSQTNLSPDLSHTTLSPELIQRNLSPALGQMPISPDLSHTTLSPDLSHTTLSLDLSQTNLSPELSQTNLSPALGQMPLSPDLSHTTLSLDFSQTNLSPELSHMTLSPELSQTNLSPALGQMPISPDLSHTTLSLDFSQTNLSPELSQTNLSPALGQMPLSPDPSHTTLSLDLSQTNLSPELSQTNLSPDLSEMPLFADLSQIPLTPDLDQMTLSPDLGETDLSPNFGQMSLSPDLSQVTLSPDISDTTLLPDLSQISPPPDLDQIFYPSESSQSLLLQEFNESFPYPDLGQMPSPSSPTLNDTFLSKEFNPLVIVGLSKDGTDYIEIIPKEEVQSSEDDYAEIDYVPYDDPYKTDVRTNINSSRDPDNIAAWYLRSNNGNRRNYYIAAEEISWDYSEFVQRETDIEDSDDIPEDTTYKKVVFRKYLDSTFTKRDPRGEYEEHLGILGPIIRAEVDDVIQVRFKNLASRPYSLHAHGLSYEKSSEGKTYEDDSPEWFKEDNAVQPNSSYTYVWHATERSGPESPGSACRAWAYYSAVNPEKDIHSGLIGPLLICQKGILHKDSNMPMDMREFVLLFMTFDEKKSWYYEKKSRSSWRLTSSEMKKSHEFHAINGMIYSLPGLKMYEQEWVRLHLLNIGGSQDIHVVHFHGQTLLENGNKQHQLGVWPLLPGSFKTLEMKASKPGWWLLNTEVGENQRAGMQTPFLIMDRDCRMPMGLSTGIISDSQIKASEFLGYWEPRLARLNNGGSYNAWSVEKLAAEFASKPWIQVDMQKEVIITGIQTQGAKHYLKSCYTTEFYVAYSSNQINWQIFKGNSTRNVMYFNGNSDASTIKENQFDPPIVARYIRISPTRAYNRPTLRLELQGCEVNGCSTPLGMENGKIENKQITASSFKKSWWGDYWEPFRARLNAQGRVNAWQAKANNNKQWLEIDLLKIKKITAIITQGCKSLSSEMYVKSYTIHYSEQGVEWKPYRLKSSMVDKIFEGNTNTKGHVKNFFNPPIISRFIRVIPKTWNQSIALRLELFGCDIY

Target ORF information:

RefSeq Version	XM_017000660.1
Organism	Homo sapiens(human)
Definition	Homo sapiens coagulation factor V (F5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000660.1

ORF Insert Sequence:

ATGGACGACG CTGTGGCTCC AGGCCGAGAA TACACCTATG AATGGAGTAT CAGTGAGGAC 
AGTGGACCCA CCCATGATGA CCCTCCATGC CTCACACACA TCTATTACTC CCATGAAAAT 
CTGATCGAGG ATTTCAACTC GGGGCTGATT GGGCCCCTGC TTATCTGTAA AAAAGGGACC 
CTAACTGAGG GTGGGACACA GAAGACGTTT GACAAGCAAA TCGTGCTACT ATTTGCTGTG 
TTTGATGAAA GCAAGAGCTG GAGCCAGTCA TCATCCCTAA TGTACACAGT CAATGGATAT 
GTGAATGGGA CAATGCCAGA TATAACAGTT TGTGCCCATG ACCACATCAG CTGGCATCTG 
CTGGGAATGA GCTCGGGGCC AGAATTATTC TCCATTCATT TCAACGGCCA GGTCCTGGAG 
CAGAACCATC ATAAGGTCTC AGCCATCACC CTTGTCAGTG CTACATCCAC TACCGCAAAT 
ATGACTGTGG GCCCAGAGGG AAAGTGGATC ATATCTTCTC TCACCCCAAA ACATTTGCAA 
GCTGGGATGC AGGCTTACAT TGACATTAAA AACTGCCCAA AGAAAACCAG GAATCTTAAG 
AAAATAACTC GTGAGCAGAG GCGGCACATG AAGAGGTGGG AATACTTCAT TGCTGCAGAG 
GAAGTCATTT GGGACTATGC ACCTGTAATA CCAGCGAATA TGGACAAAAA ATACAGGTCT 
CAGCATTTGG ATAATTTCTC AAACCAAATT GGAAAACATT ATAAGAAAGT TATGTACACA 
CAGTACGAAG ATGAGTCCTT CACCAAACAT ACAGTGAATC CCAATATGAA AGAAGATGGG 
ATTTTGGGTC CTATTATCAG AGCCCAGGTC AGAGACACAC TCAAAATCGT GTTCAAAAAT 
ATGGCCAGCC GCCCCTATAG CATTTACCCT CATGGAGTGA CCTTCTCGCC TTATGAAGAT 
GAAGTCAACT CTTCTTTCAC CTCAGGCAGG AACAACACCA TGATCAGAGC AGTTCAACCA 
GGGGAAACCT ATACTTATAA GTGGAACATC TTAGAGTTTG ATGAACCCAC AGAAAATGAT 
GCCCAGTGCT TAACAAGACC ATACTACAGT GACGTGGACA TCATGAGAGA CATCGCCTCT 
GGGCTAATAG GACTACTTCT AATCTGTAAG AGCAGATCCC TGGACAGGCG AGGAATACAG 
AGGGCAGCAG ACATCGAACA GCAGGCTGTG TTTGCTGTGT TTGATGAGAA CAAAAGCTGG 
TACCTTGAGG ACAACATCAA CAAGTTTTGT GAAAATCCTG ATGAGGTGAA ACGTGATGAC 
CCCAAGTTTT ATGAATCAAA CATCATGAGC ACTATCAATG GCTATGTGCC TGAGAGCATA 
ACTACTCTTG GATTCTGCTT TGATGACACT GTCCAGTGGC ACTTCTGTAG TGTGGGGACC 
CAGAATGAAA TTTTGACCAT CCACTTCACT GGGCACTCAT TCATCTATGG AAAGAGGCAT 
GAGGACACCT TGACCCTCTT CCCCATGCGT GGAGAATCTG TGACGGTCAC AATGGATAAT 
GTTGGAACTT GGATGTTAAC TTCCATGAAT TCTAGTCCAA GAAGCAAAAA GCTGAGGCTG 
AAATTCAGGG ATGTTAAATG TATCCCAGAT GATGATGAAG ACTCATATGA GATTTTTGAA 
CCTCCAGAAT CTACAGTCAT GGCTACACGG AAAATGCATG ATCGTTTAGA ACCTGAAGAT 
GAAGAGAGTG ATGCTGACTA TGATTACCAG AACAGACTGG CTGCAGCATT AGGAATCAGG 
TCATTCCGAA ACTCATCATT GAATCAGGAA GAAGAAGAGT TCAATCTTAC TGCCCTAGCT 
CTGGAGAATG GCACTGAATT CGTTTCTTCA AACACAGATA TAATTGTTGG TTCAAATTAT 
TCTTCCCCAA GTAATATTAG TAAGTTCACT GTCAATAACC TTGCAGAACC TCAGAAAGCC 
CCTTCTCACC AACAAGCCAC CACAGCTGGT TCCCCACTGA GACACCTCAT TGGCAAGAAC 
TCAGTTCTCA ATTCTTCCAC AGCAGAGCAT TCCAGCCCAT ATTCTGAAGA CCCTATAGAG 
GATCCTCTAC AGCCAGATGT CACAGGGATA CGTCTACTTT CACTTGGTGC TGGAGAATTC 
AAAAGTCAAG AACATGCTAA GCATAAGGGA CCCAAGGTAG AAAGAGATCA AGCAGCAAAG 
CACAGGTTCT CCTGGATGAA ATTACTAGCA CATAAAGTTG GGAGACACCT AAGCCAAGAC 
ACTGGTTCTC CTTCCGGAAT GAGGCCCTGG GAGGACCTTC CTAGCCAAGA CACTGGTTCT 
CCTTCCAGAA TGAGGCCCTG GAAGGACCCT CCTAGTGATC TGTTACTCTT AAAACAAAGT 
AACTCATCTA AGATTTTGGT TGGGAGATGG CATTTGGCTT CTGAGAAAGG TAGCTATGAA 
ATAATCCAAG ATACTGATGA AGACACAGCT GTTAACAATT GGCTGATCAG CCCCCAGAAT 
GCCTCACGTG CTTGGGGAGA AAGCACCCCT CTTGCCAACA AGCCTGGAAA GCAGAGTGGC 
CACCCAAAGT TTCCTAGAGT TAGACATAAA TCTCTACAAG TAAGACAGGA TGGAGGAAAG 
AGTAGACTGA AGAAAAGCCA GTTTCTCATT AAGACACGAA AAAAGAAAAA AGAGAAGCAC 
ACACACCATG CTCCTTTATC TCCGAGGACC TTTCACCCTC TAAGAAGTGA AGCCTACAAC 
ACATTTTCAG AAAGAAGACT TAAGCATTCG TTGGTGCTTC ATAAATCCAA TGAAACATCT 
CTTCCCACAG ACCTCAATCA GACATTGCCC TCTATGGATT TTGGCTGGAT AGCCTCACTT 
CCTGACCATA ATCAGAATTC CTCAAATGAC ACTGGTCAGG CAAGCTGTCC TCCAGGTCTT 
TATCAGACAG TGCCCCCAGA GGAACACTAT CAAACATTCC CCATTCAAGA CCCTGATCAA 
ATGCACTCTA CTTCAGACCC CAGTCACAGA TCCTCTTCTC CAGAGCTCAG TGAAATGCTT 
GAGTATGACC GAAGTCACAA GTCCTTCCCC ACAGATATAA GTCAAATGTC CCCTTCCTCA 
GAACATGAAG TCTGGCAGAC AGTCATCTCT CCAGACCTCA GCCAGGTGAC CCTCTCTCCA 
GAACTCAGCC AGACAAACCT CTCTCCAGAC CTCAGCCACA CGACTCTCTC TCCAGAACTC 
ATTCAGAGAA ACCTTTCCCC AGCCCTCGGT CAGATGCCCA TTTCTCCAGA CCTCAGCCAT 
ACAACCCTTT CTCCAGACCT CAGCCATACA ACCCTTTCTT TAGACCTCAG CCAGACAAAC 
CTCTCTCCAG AACTCAGTCA GACAAACCTT TCTCCAGCCC TCGGTCAGAT GCCCCTTTCT 
CCAGACCTCA GCCATACAAC CCTTTCTCTA GACTTCAGCC AGACAAACCT CTCTCCAGAA 
CTCAGCCATA TGACTCTCTC TCCAGAACTC AGTCAGACAA ACCTTTCCCC AGCCCTCGGT 
CAGATGCCCA TTTCTCCAGA CCTCAGCCAT ACAACCCTTT CTCTAGACTT CAGCCAGACA 
AACCTCTCTC CAGAACTCAG TCAAACAAAC CTTTCCCCAG CCCTCGGTCA GATGCCCCTT 
TCTCCAGACC CCAGCCATAC AACCCTTTCT CTAGACCTCA GCCAGACAAA CCTCTCTCCA 
GAACTCAGTC AGACAAACCT TTCCCCAGAC CTCAGTGAGA TGCCCCTCTT TGCAGATCTC 
AGTCAAATTC CCCTTACCCC AGACCTCGAC CAGATGACAC TTTCTCCAGA CCTTGGTGAG 
ACAGATCTTT CCCCAAACTT TGGTCAGATG TCCCTTTCCC CAGACCTCAG CCAGGTGACT 
CTCTCTCCAG ACATCAGTGA CACCACCCTT CTCCCGGATC TCAGCCAGAT ATCACCTCCT 
CCAGACCTTG ATCAGATATT CTACCCTTCT GAATCTAGTC AGTCATTGCT TCTTCAAGAA 
TTTAATGAGT CTTTTCCTTA TCCAGACCTT GGTCAGATGC CATCTCCTTC ATCTCCTACT 
CTCAATGATA CTTTTCTATC AAAGGAATTT AATCCACTGG TTATAGTGGG CCTCAGTAAA 
GATGGTACAG ATTACATTGA GATCATTCCA AAGGAAGAGG TCCAGAGCAG TGAAGATGAC 
TATGCTGAAA TTGATTATGT GCCCTATGAT GACCCCTACA AAACTGATGT TAGGACAAAC 
ATCAACTCCT CCAGAGATCC TGACAACATT GCAGCATGGT ACCTCCGCAG CAACAATGGA 
AACAGAAGAA ATTATTACAT TGCTGCTGAA GAAATATCCT GGGATTATTC AGAATTTGTA 
CAAAGGGAAA CAGATATTGA AGACTCTGAT GATATTCCAG AAGATACCAC ATATAAGAAA 
GTAGTTTTTC GAAAGTACCT CGACAGCACT TTTACCAAAC GTGATCCTCG AGGGGAGTAT 
GAAGAGCATC TCGGAATTCT TGGTCCTATT ATCAGAGCTG AAGTGGATGA TGTTATCCAA 
GTTCGTTTTA AAAATTTAGC ATCCAGACCG TATTCTCTAC ATGCCCATGG ACTTTCCTAT 
GAAAAATCAT CAGAGGGAAA GACTTATGAA GATGACTCTC CTGAATGGTT TAAGGAAGAT 
AATGCTGTTC AGCCAAATAG CAGTTATACC TACGTATGGC ATGCCACTGA GCGATCAGGG 
CCAGAAAGTC CTGGCTCTGC CTGTCGGGCT TGGGCCTACT ACTCAGCTGT GAACCCAGAA 
AAAGATATTC ACTCAGGCTT GATAGGTCCC CTCCTAATCT GCCAAAAAGG AATACTACAT 
AAGGACAGCA ACATGCCTAT GGACATGAGA GAATTTGTCT TACTATTTAT GACCTTTGAT 
GAAAAGAAGA GCTGGTACTA TGAAAAGAAG TCCCGAAGTT CTTGGAGACT CACATCCTCA 
GAAATGAAAA AATCCCATGA GTTTCACGCC ATTAATGGGA TGATCTACAG CTTGCCTGGC 
CTGAAAATGT ATGAGCAAGA GTGGGTGAGG TTACACCTGC TGAACATAGG CGGCTCCCAA 
GACATTCACG TGGTTCACTT TCACGGCCAG ACCTTGCTGG AAAATGGCAA TAAACAGCAC 
CAGTTAGGGG TCTGGCCCCT TCTGCCTGGT TCATTTAAAA CTCTTGAAAT GAAGGCATCA 
AAACCTGGCT GGTGGCTCCT AAACACAGAG GTTGGAGAAA ACCAGAGAGC AGGGATGCAA 
ACGCCATTTC TTATCATGGA CAGAGACTGT AGGATGCCAA TGGGACTAAG CACTGGTATC 
ATATCTGATT CACAGATCAA GGCTTCAGAG TTTCTGGGTT ACTGGGAGCC CAGATTAGCA 
AGATTAAACA ATGGTGGATC TTATAATGCT TGGAGTGTAG AAAAACTTGC AGCAGAATTT 
GCCTCTAAAC CTTGGATCCA GGTGGACATG CAAAAGGAAG TCATAATCAC AGGGATCCAG 
ACCCAAGGTG CCAAACACTA CCTGAAGTCC TGCTATACCA CAGAGTTCTA TGTAGCTTAC 
AGTTCCAACC AGATCAACTG GCAGATCTTC AAAGGGAACA GCACAAGGAA TGTGATGTAT 
TTTAATGGCA ATTCAGATGC CTCTACAATA AAAGAGAATC AGTTTGACCC ACCTATTGTG 
GCTAGATATA TTAGGATCTC TCCAACTCGA GCCTATAACA GACCTACCCT TCGATTGGAA 
CTGCAAGGTT GTGAGGTAAA TGGATGTTCC ACACCCCTGG GTATGGAAAA TGGAAAGATA 
GAAAACAAGC AAATCACAGC TTCTTCGTTT AAGAAATCTT GGTGGGGAGA TTACTGGGAA 
CCCTTCCGTG CCCGTCTGAA TGCCCAGGGA CGTGTGAATG CCTGGCAAGC CAAGGCAAAC 
AACAATAAGC AGTGGCTAGA AATTGATCTA CTCAAGATCA AGAAGATAAC GGCAATTATA 
ACACAGGGCT GCAAGTCTCT GTCCTCTGAA ATGTATGTAA AGAGCTATAC CATCCACTAC 
AGTGAGCAGG GAGTGGAATG GAAACCATAC AGGCTGAAAT CCTCCATGGT GGACAAGATT 
TTTGAAGGAA ATACTAATAC CAAAGGACAT GTGAAGAACT TTTTCAACCC CCCAATCATT 
TCCAGGTTTA TCCGTGTCAT TCCTAAAACA TGGAATCAAA GTATTGCACT TCGCCTGGAA 
CTCTTTGGCT GTGATATTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F5 ( XM_017000660.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856149.1 Homo sapiens coagulation factor V (F5), transcript variant X1, mRNA.

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CloneID	OHu83340
Clone ID Related Accession (Same CDS sequence)	XM_017000660.1 , XM_017000660.2
Accession Version	XM_017000660.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	coagulation factor V isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017000660.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGACG CTGTGGCTCC AGGCCGAGAA TACACCTATG AATGGAGTAT CAGTGAGGAC 
AGTGGACCCA CCCATGATGA CCCTCCATGC CTCACACACA TCTATTACTC CCATGAAAAT 
CTGATCGAGG ATTTCAACTC GGGGCTGATT GGGCCCCTGC TTATCTGTAA AAAAGGGACC 
CTAACTGAGG GTGGGACACA GAAGACGTTT GACAAGCAAA TCGTGCTACT ATTTGCTGTG 
TTTGATGAAA GCAAGAGCTG GAGCCAGTCA TCATCCCTAA TGTACACAGT CAATGGATAT 
GTGAATGGGA CAATGCCAGA TATAACAGTT TGTGCCCATG ACCACATCAG CTGGCATCTG 
CTGGGAATGA GCTCGGGGCC AGAATTATTC TCCATTCATT TCAACGGCCA GGTCCTGGAG 
CAGAACCATC ATAAGGTCTC AGCCATCACC CTTGTCAGTG CTACATCCAC TACCGCAAAT 
ATGACTGTGG GCCCAGAGGG AAAGTGGATC ATATCTTCTC TCACCCCAAA ACATTTGCAA 
GCTGGGATGC AGGCTTACAT TGACATTAAA AACTGCCCAA AGAAAACCAG GAATCTTAAG 
AAAATAACTC GTGAGCAGAG GCGGCACATG AAGAGGTGGG AATACTTCAT TGCTGCAGAG 
GAAGTCATTT GGGACTATGC ACCTGTAATA CCAGCGAATA TGGACAAAAA ATACAGGTCT 
CAGCATTTGG ATAATTTCTC AAACCAAATT GGAAAACATT ATAAGAAAGT TATGTACACA 
CAGTACGAAG ATGAGTCCTT CACCAAACAT ACAGTGAATC CCAATATGAA AGAAGATGGG 
ATTTTGGGTC CTATTATCAG AGCCCAGGTC AGAGACACAC TCAAAATCGT GTTCAAAAAT 
ATGGCCAGCC GCCCCTATAG CATTTACCCT CATGGAGTGA CCTTCTCGCC TTATGAAGAT 
GAAGTCAACT CTTCTTTCAC CTCAGGCAGG AACAACACCA TGATCAGAGC AGTTCAACCA 
GGGGAAACCT ATACTTATAA GTGGAACATC TTAGAGTTTG ATGAACCCAC AGAAAATGAT 
GCCCAGTGCT TAACAAGACC ATACTACAGT GACGTGGACA TCATGAGAGA CATCGCCTCT 
GGGCTAATAG GACTACTTCT AATCTGTAAG AGCAGATCCC TGGACAGGCG AGGAATACAG 
AGGGCAGCAG ACATCGAACA GCAGGCTGTG TTTGCTGTGT TTGATGAGAA CAAAAGCTGG 
TACCTTGAGG ACAACATCAA CAAGTTTTGT GAAAATCCTG ATGAGGTGAA ACGTGATGAC 
CCCAAGTTTT ATGAATCAAA CATCATGAGC ACTATCAATG GCTATGTGCC TGAGAGCATA 
ACTACTCTTG GATTCTGCTT TGATGACACT GTCCAGTGGC ACTTCTGTAG TGTGGGGACC 
CAGAATGAAA TTTTGACCAT CCACTTCACT GGGCACTCAT TCATCTATGG AAAGAGGCAT 
GAGGACACCT TGACCCTCTT CCCCATGCGT GGAGAATCTG TGACGGTCAC AATGGATAAT 
GTTGGAACTT GGATGTTAAC TTCCATGAAT TCTAGTCCAA GAAGCAAAAA GCTGAGGCTG 
AAATTCAGGG ATGTTAAATG TATCCCAGAT GATGATGAAG ACTCATATGA GATTTTTGAA 
CCTCCAGAAT CTACAGTCAT GGCTACACGG AAAATGCATG ATCGTTTAGA ACCTGAAGAT 
GAAGAGAGTG ATGCTGACTA TGATTACCAG AACAGACTGG CTGCAGCATT AGGAATCAGG 
TCATTCCGAA ACTCATCATT GAATCAGGAA GAAGAAGAGT TCAATCTTAC TGCCCTAGCT 
CTGGAGAATG GCACTGAATT CGTTTCTTCA AACACAGATA TAATTGTTGG TTCAAATTAT 
TCTTCCCCAA GTAATATTAG TAAGTTCACT GTCAATAACC TTGCAGAACC TCAGAAAGCC 
CCTTCTCACC AACAAGCCAC CACAGCTGGT TCCCCACTGA GACACCTCAT TGGCAAGAAC 
TCAGTTCTCA ATTCTTCCAC AGCAGAGCAT TCCAGCCCAT ATTCTGAAGA CCCTATAGAG 
GATCCTCTAC AGCCAGATGT CACAGGGATA CGTCTACTTT CACTTGGTGC TGGAGAATTC 
AAAAGTCAAG AACATGCTAA GCATAAGGGA CCCAAGGTAG AAAGAGATCA AGCAGCAAAG 
CACAGGTTCT CCTGGATGAA ATTACTAGCA CATAAAGTTG GGAGACACCT AAGCCAAGAC 
ACTGGTTCTC CTTCCGGAAT GAGGCCCTGG GAGGACCTTC CTAGCCAAGA CACTGGTTCT 
CCTTCCAGAA TGAGGCCCTG GAAGGACCCT CCTAGTGATC TGTTACTCTT AAAACAAAGT 
AACTCATCTA AGATTTTGGT TGGGAGATGG CATTTGGCTT CTGAGAAAGG TAGCTATGAA 
ATAATCCAAG ATACTGATGA AGACACAGCT GTTAACAATT GGCTGATCAG CCCCCAGAAT 
GCCTCACGTG CTTGGGGAGA AAGCACCCCT CTTGCCAACA AGCCTGGAAA GCAGAGTGGC 
CACCCAAAGT TTCCTAGAGT TAGACATAAA TCTCTACAAG TAAGACAGGA TGGAGGAAAG 
AGTAGACTGA AGAAAAGCCA GTTTCTCATT AAGACACGAA AAAAGAAAAA AGAGAAGCAC 
ACACACCATG CTCCTTTATC TCCGAGGACC TTTCACCCTC TAAGAAGTGA AGCCTACAAC 
ACATTTTCAG AAAGAAGACT TAAGCATTCG TTGGTGCTTC ATAAATCCAA TGAAACATCT 
CTTCCCACAG ACCTCAATCA GACATTGCCC TCTATGGATT TTGGCTGGAT AGCCTCACTT 
CCTGACCATA ATCAGAATTC CTCAAATGAC ACTGGTCAGG CAAGCTGTCC TCCAGGTCTT 
TATCAGACAG TGCCCCCAGA GGAACACTAT CAAACATTCC CCATTCAAGA CCCTGATCAA 
ATGCACTCTA CTTCAGACCC CAGTCACAGA TCCTCTTCTC CAGAGCTCAG TGAAATGCTT 
GAGTATGACC GAAGTCACAA GTCCTTCCCC ACAGATATAA GTCAAATGTC CCCTTCCTCA 
GAACATGAAG TCTGGCAGAC AGTCATCTCT CCAGACCTCA GCCAGGTGAC CCTCTCTCCA 
GAACTCAGCC AGACAAACCT CTCTCCAGAC CTCAGCCACA CGACTCTCTC TCCAGAACTC 
ATTCAGAGAA ACCTTTCCCC AGCCCTCGGT CAGATGCCCA TTTCTCCAGA CCTCAGCCAT 
ACAACCCTTT CTCCAGACCT CAGCCATACA ACCCTTTCTT TAGACCTCAG CCAGACAAAC 
CTCTCTCCAG AACTCAGTCA GACAAACCTT TCTCCAGCCC TCGGTCAGAT GCCCCTTTCT 
CCAGACCTCA GCCATACAAC CCTTTCTCTA GACTTCAGCC AGACAAACCT CTCTCCAGAA 
CTCAGCCATA TGACTCTCTC TCCAGAACTC AGTCAGACAA ACCTTTCCCC AGCCCTCGGT 
CAGATGCCCA TTTCTCCAGA CCTCAGCCAT ACAACCCTTT CTCTAGACTT CAGCCAGACA 
AACCTCTCTC CAGAACTCAG TCAAACAAAC CTTTCCCCAG CCCTCGGTCA GATGCCCCTT 
TCTCCAGACC CCAGCCATAC AACCCTTTCT CTAGACCTCA GCCAGACAAA CCTCTCTCCA 
GAACTCAGTC AGACAAACCT TTCCCCAGAC CTCAGTGAGA TGCCCCTCTT TGCAGATCTC 
AGTCAAATTC CCCTTACCCC AGACCTCGAC CAGATGACAC TTTCTCCAGA CCTTGGTGAG 
ACAGATCTTT CCCCAAACTT TGGTCAGATG TCCCTTTCCC CAGACCTCAG CCAGGTGACT 
CTCTCTCCAG ACATCAGTGA CACCACCCTT CTCCCGGATC TCAGCCAGAT ATCACCTCCT 
CCAGACCTTG ATCAGATATT CTACCCTTCT GAATCTAGTC AGTCATTGCT TCTTCAAGAA 
TTTAATGAGT CTTTTCCTTA TCCAGACCTT GGTCAGATGC CATCTCCTTC ATCTCCTACT 
CTCAATGATA CTTTTCTATC AAAGGAATTT AATCCACTGG TTATAGTGGG CCTCAGTAAA 
GATGGTACAG ATTACATTGA GATCATTCCA AAGGAAGAGG TCCAGAGCAG TGAAGATGAC 
TATGCTGAAA TTGATTATGT GCCCTATGAT GACCCCTACA AAACTGATGT TAGGACAAAC 
ATCAACTCCT CCAGAGATCC TGACAACATT GCAGCATGGT ACCTCCGCAG CAACAATGGA 
AACAGAAGAA ATTATTACAT TGCTGCTGAA GAAATATCCT GGGATTATTC AGAATTTGTA 
CAAAGGGAAA CAGATATTGA AGACTCTGAT GATATTCCAG AAGATACCAC ATATAAGAAA 
GTAGTTTTTC GAAAGTACCT CGACAGCACT TTTACCAAAC GTGATCCTCG AGGGGAGTAT 
GAAGAGCATC TCGGAATTCT TGGTCCTATT ATCAGAGCTG AAGTGGATGA TGTTATCCAA 
GTTCGTTTTA AAAATTTAGC ATCCAGACCG TATTCTCTAC ATGCCCATGG ACTTTCCTAT 
GAAAAATCAT CAGAGGGAAA GACTTATGAA GATGACTCTC CTGAATGGTT TAAGGAAGAT 
AATGCTGTTC AGCCAAATAG CAGTTATACC TACGTATGGC ATGCCACTGA GCGATCAGGG 
CCAGAAAGTC CTGGCTCTGC CTGTCGGGCT TGGGCCTACT ACTCAGCTGT GAACCCAGAA 
AAAGATATTC ACTCAGGCTT GATAGGTCCC CTCCTAATCT GCCAAAAAGG AATACTACAT 
AAGGACAGCA ACATGCCTAT GGACATGAGA GAATTTGTCT TACTATTTAT GACCTTTGAT 
GAAAAGAAGA GCTGGTACTA TGAAAAGAAG TCCCGAAGTT CTTGGAGACT CACATCCTCA 
GAAATGAAAA AATCCCATGA GTTTCACGCC ATTAATGGGA TGATCTACAG CTTGCCTGGC 
CTGAAAATGT ATGAGCAAGA GTGGGTGAGG TTACACCTGC TGAACATAGG CGGCTCCCAA 
GACATTCACG TGGTTCACTT TCACGGCCAG ACCTTGCTGG AAAATGGCAA TAAACAGCAC 
CAGTTAGGGG TCTGGCCCCT TCTGCCTGGT TCATTTAAAA CTCTTGAAAT GAAGGCATCA 
AAACCTGGCT GGTGGCTCCT AAACACAGAG GTTGGAGAAA ACCAGAGAGC AGGGATGCAA 
ACGCCATTTC TTATCATGGA CAGAGACTGT AGGATGCCAA TGGGACTAAG CACTGGTATC 
ATATCTGATT CACAGATCAA GGCTTCAGAG TTTCTGGGTT ACTGGGAGCC CAGATTAGCA 
AGATTAAACA ATGGTGGATC TTATAATGCT TGGAGTGTAG AAAAACTTGC AGCAGAATTT 
GCCTCTAAAC CTTGGATCCA GGTGGACATG CAAAAGGAAG TCATAATCAC AGGGATCCAG 
ACCCAAGGTG CCAAACACTA CCTGAAGTCC TGCTATACCA CAGAGTTCTA TGTAGCTTAC 
AGTTCCAACC AGATCAACTG GCAGATCTTC AAAGGGAACA GCACAAGGAA TGTGATGTAT 
TTTAATGGCA ATTCAGATGC CTCTACAATA AAAGAGAATC AGTTTGACCC ACCTATTGTG 
GCTAGATATA TTAGGATCTC TCCAACTCGA GCCTATAACA GACCTACCCT TCGATTGGAA 
CTGCAAGGTT GTGAGGTAAA TGGATGTTCC ACACCCCTGG GTATGGAAAA TGGAAAGATA 
GAAAACAAGC AAATCACAGC TTCTTCGTTT AAGAAATCTT GGTGGGGAGA TTACTGGGAA 
CCCTTCCGTG CCCGTCTGAA TGCCCAGGGA CGTGTGAATG CCTGGCAAGC CAAGGCAAAC 
AACAATAAGC AGTGGCTAGA AATTGATCTA CTCAAGATCA AGAAGATAAC GGCAATTATA 
ACACAGGGCT GCAAGTCTCT GTCCTCTGAA ATGTATGTAA AGAGCTATAC CATCCACTAC 
AGTGAGCAGG GAGTGGAATG GAAACCATAC AGGCTGAAAT CCTCCATGGT GGACAAGATT 
TTTGAAGGAA ATACTAATAC CAAAGGACAT GTGAAGAACT TTTTCAACCC CCCAATCATT 
TCCAGGTTTA TCCGTGTCAT TCCTAAAACA TGGAATCAAA GTATTGCACT TCGCCTGGAA 
CTCTTTGGCT GTGATATTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856149.1
CDS	564..6827
Translation	MDDAVAPGREYTYEWSISEDSGPTHDDPPCLTHIYYSHENLIEDFNSGLIGPLLICKKGTLTEGGTQKTFDKQIVLLFAVFDESKSWSQSSSLMYTVNGYVNGTMPDITVCAHDHISWHLLGMSSGPELFSIHFNGQVLEQNHHKVSAITLVSATSTTANMTVGPEGKWIISSLTPKHLQAGMQAYIDIKNCPKKTRNLKKITREQRRHMKRWEYFIAAEEVIWDYAPVIPANMDKKYRSQHLDNFSNQIGKHYKKVMYTQYEDESFTKHTVNPNMKEDGILGPIIRAQVRDTLKIVFKNMASRPYSIYPHGVTFSPYEDEVNSSFTSGRNNTMIRAVQPGETYTYKWNILEFDEPTENDAQCLTRPYYSDVDIMRDIASGLIGLLLICKSRSLDRRGIQRAADIEQQAVFAVFDENKSWYLEDNINKFCENPDEVKRDDPKFYESNIMSTINGYVPESITTLGFCFDDTVQWHFCSVGTQNEILTIHFTGHSFIYGKRHEDTLTLFPMRGESVTVTMDNVGTWMLTSMNSSPRSKKLRLKFRDVKCIPDDDEDSYEIFEPPESTVMATRKMHDRLEPEDEESDADYDYQNRLAAALGIRSFRNSSLNQEEEEFNLTALALENGTEFVSSNTDIIVGSNYSSPSNISKFTVNNLAEPQKAPSHQQATTAGSPLRHLIGKNSVLNSSTAEHSSPYSEDPIEDPLQPDVTGIRLLSLGAGEFKSQEHAKHKGPKVERDQAAKHRFSWMKLLAHKVGRHLSQDTGSPSGMRPWEDLPSQDTGSPSRMRPWKDPPSDLLLLKQSNSSKILVGRWHLASEKGSYEIIQDTDEDTAVNNWLISPQNASRAWGESTPLANKPGKQSGHPKFPRVRHKSLQVRQDGGKSRLKKSQFLIKTRKKKKEKHTHHAPLSPRTFHPLRSEAYNTFSERRLKHSLVLHKSNETSLPTDLNQTLPSMDFGWIASLPDHNQNSSNDTGQASCPPGLYQTVPPEEHYQTFPIQDPDQMHSTSDPSHRSSSPELSEMLEYDRSHKSFPTDISQMSPSSEHEVWQTVISPDLSQVTLSPELSQTNLSPDLSHTTLSPELIQRNLSPALGQMPISPDLSHTTLSPDLSHTTLSLDLSQTNLSPELSQTNLSPALGQMPLSPDLSHTTLSLDFSQTNLSPELSHMTLSPELSQTNLSPALGQMPISPDLSHTTLSLDFSQTNLSPELSQTNLSPALGQMPLSPDPSHTTLSLDLSQTNLSPELSQTNLSPDLSEMPLFADLSQIPLTPDLDQMTLSPDLGETDLSPNFGQMSLSPDLSQVTLSPDISDTTLLPDLSQISPPPDLDQIFYPSESSQSLLLQEFNESFPYPDLGQMPSPSSPTLNDTFLSKEFNPLVIVGLSKDGTDYIEIIPKEEVQSSEDDYAEIDYVPYDDPYKTDVRTNINSSRDPDNIAAWYLRSNNGNRRNYYIAAEEISWDYSEFVQRETDIEDSDDIPEDTTYKKVVFRKYLDSTFTKRDPRGEYEEHLGILGPIIRAEVDDVIQVRFKNLASRPYSLHAHGLSYEKSSEGKTYEDDSPEWFKEDNAVQPNSSYTYVWHATERSGPESPGSACRAWAYYSAVNPEKDIHSGLIGPLLICQKGILHKDSNMPMDMREFVLLFMTFDEKKSWYYEKKSRSSWRLTSSEMKKSHEFHAINGMIYSLPGLKMYEQEWVRLHLLNIGGSQDIHVVHFHGQTLLENGNKQHQLGVWPLLPGSFKTLEMKASKPGWWLLNTEVGENQRAGMQTPFLIMDRDCRMPMGLSTGIISDSQIKASEFLGYWEPRLARLNNGGSYNAWSVEKLAAEFASKPWIQVDMQKEVIITGIQTQGAKHYLKSCYTTEFYVAYSSNQINWQIFKGNSTRNVMYFNGNSDASTIKENQFDPPIVARYIRISPTRAYNRPTLRLELQGCEVNGCSTPLGMENGKIENKQITASSFKKSWWGDYWEPFRARLNAQGRVNAWQAKANNNKQWLEIDLLKIKKITAIITQGCKSLSSEMYVKSYTIHYSEQGVEWKPYRLKSSMVDKIFEGNTNTKGHVKNFFNPPIISRFIRVIPKTWNQSIALRLELFGCDIY

Target ORF information:

RefSeq Version	XM_017000660.2
Organism	Homo sapiens(human)
Definition	Homo sapiens coagulation factor V (F5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000660.2

ORF Insert Sequence:

ATGGACGACG CTGTGGCTCC AGGCCGAGAA TACACCTATG AATGGAGTAT CAGTGAGGAC 
AGTGGACCCA CCCATGATGA CCCTCCATGC CTCACACACA TCTATTACTC CCATGAAAAT 
CTGATCGAGG ATTTCAACTC GGGGCTGATT GGGCCCCTGC TTATCTGTAA AAAAGGGACC 
CTAACTGAGG GTGGGACACA GAAGACGTTT GACAAGCAAA TCGTGCTACT ATTTGCTGTG 
TTTGATGAAA GCAAGAGCTG GAGCCAGTCA TCATCCCTAA TGTACACAGT CAATGGATAT 
GTGAATGGGA CAATGCCAGA TATAACAGTT TGTGCCCATG ACCACATCAG CTGGCATCTG 
CTGGGAATGA GCTCGGGGCC AGAATTATTC TCCATTCATT TCAACGGCCA GGTCCTGGAG 
CAGAACCATC ATAAGGTCTC AGCCATCACC CTTGTCAGTG CTACATCCAC TACCGCAAAT 
ATGACTGTGG GCCCAGAGGG AAAGTGGATC ATATCTTCTC TCACCCCAAA ACATTTGCAA 
GCTGGGATGC AGGCTTACAT TGACATTAAA AACTGCCCAA AGAAAACCAG GAATCTTAAG 
AAAATAACTC GTGAGCAGAG GCGGCACATG AAGAGGTGGG AATACTTCAT TGCTGCAGAG 
GAAGTCATTT GGGACTATGC ACCTGTAATA CCAGCGAATA TGGACAAAAA ATACAGGTCT 
CAGCATTTGG ATAATTTCTC AAACCAAATT GGAAAACATT ATAAGAAAGT TATGTACACA 
CAGTACGAAG ATGAGTCCTT CACCAAACAT ACAGTGAATC CCAATATGAA AGAAGATGGG 
ATTTTGGGTC CTATTATCAG AGCCCAGGTC AGAGACACAC TCAAAATCGT GTTCAAAAAT 
ATGGCCAGCC GCCCCTATAG CATTTACCCT CATGGAGTGA CCTTCTCGCC TTATGAAGAT 
GAAGTCAACT CTTCTTTCAC CTCAGGCAGG AACAACACCA TGATCAGAGC AGTTCAACCA 
GGGGAAACCT ATACTTATAA GTGGAACATC TTAGAGTTTG ATGAACCCAC AGAAAATGAT 
GCCCAGTGCT TAACAAGACC ATACTACAGT GACGTGGACA TCATGAGAGA CATCGCCTCT 
GGGCTAATAG GACTACTTCT AATCTGTAAG AGCAGATCCC TGGACAGGCG AGGAATACAG 
AGGGCAGCAG ACATCGAACA GCAGGCTGTG TTTGCTGTGT TTGATGAGAA CAAAAGCTGG 
TACCTTGAGG ACAACATCAA CAAGTTTTGT GAAAATCCTG ATGAGGTGAA ACGTGATGAC 
CCCAAGTTTT ATGAATCAAA CATCATGAGC ACTATCAATG GCTATGTGCC TGAGAGCATA 
ACTACTCTTG GATTCTGCTT TGATGACACT GTCCAGTGGC ACTTCTGTAG TGTGGGGACC 
CAGAATGAAA TTTTGACCAT CCACTTCACT GGGCACTCAT TCATCTATGG AAAGAGGCAT 
GAGGACACCT TGACCCTCTT CCCCATGCGT GGAGAATCTG TGACGGTCAC AATGGATAAT 
GTTGGAACTT GGATGTTAAC TTCCATGAAT TCTAGTCCAA GAAGCAAAAA GCTGAGGCTG 
AAATTCAGGG ATGTTAAATG TATCCCAGAT GATGATGAAG ACTCATATGA GATTTTTGAA 
CCTCCAGAAT CTACAGTCAT GGCTACACGG AAAATGCATG ATCGTTTAGA ACCTGAAGAT 
GAAGAGAGTG ATGCTGACTA TGATTACCAG AACAGACTGG CTGCAGCATT AGGAATCAGG 
TCATTCCGAA ACTCATCATT GAATCAGGAA GAAGAAGAGT TCAATCTTAC TGCCCTAGCT 
CTGGAGAATG GCACTGAATT CGTTTCTTCA AACACAGATA TAATTGTTGG TTCAAATTAT 
TCTTCCCCAA GTAATATTAG TAAGTTCACT GTCAATAACC TTGCAGAACC TCAGAAAGCC 
CCTTCTCACC AACAAGCCAC CACAGCTGGT TCCCCACTGA GACACCTCAT TGGCAAGAAC 
TCAGTTCTCA ATTCTTCCAC AGCAGAGCAT TCCAGCCCAT ATTCTGAAGA CCCTATAGAG 
GATCCTCTAC AGCCAGATGT CACAGGGATA CGTCTACTTT CACTTGGTGC TGGAGAATTC 
AAAAGTCAAG AACATGCTAA GCATAAGGGA CCCAAGGTAG AAAGAGATCA AGCAGCAAAG 
CACAGGTTCT CCTGGATGAA ATTACTAGCA CATAAAGTTG GGAGACACCT AAGCCAAGAC 
ACTGGTTCTC CTTCCGGAAT GAGGCCCTGG GAGGACCTTC CTAGCCAAGA CACTGGTTCT 
CCTTCCAGAA TGAGGCCCTG GAAGGACCCT CCTAGTGATC TGTTACTCTT AAAACAAAGT 
AACTCATCTA AGATTTTGGT TGGGAGATGG CATTTGGCTT CTGAGAAAGG TAGCTATGAA 
ATAATCCAAG ATACTGATGA AGACACAGCT GTTAACAATT GGCTGATCAG CCCCCAGAAT 
GCCTCACGTG CTTGGGGAGA AAGCACCCCT CTTGCCAACA AGCCTGGAAA GCAGAGTGGC 
CACCCAAAGT TTCCTAGAGT TAGACATAAA TCTCTACAAG TAAGACAGGA TGGAGGAAAG 
AGTAGACTGA AGAAAAGCCA GTTTCTCATT AAGACACGAA AAAAGAAAAA AGAGAAGCAC 
ACACACCATG CTCCTTTATC TCCGAGGACC TTTCACCCTC TAAGAAGTGA AGCCTACAAC 
ACATTTTCAG AAAGAAGACT TAAGCATTCG TTGGTGCTTC ATAAATCCAA TGAAACATCT 
CTTCCCACAG ACCTCAATCA GACATTGCCC TCTATGGATT TTGGCTGGAT AGCCTCACTT 
CCTGACCATA ATCAGAATTC CTCAAATGAC ACTGGTCAGG CAAGCTGTCC TCCAGGTCTT 
TATCAGACAG TGCCCCCAGA GGAACACTAT CAAACATTCC CCATTCAAGA CCCTGATCAA 
ATGCACTCTA CTTCAGACCC CAGTCACAGA TCCTCTTCTC CAGAGCTCAG TGAAATGCTT 
GAGTATGACC GAAGTCACAA GTCCTTCCCC ACAGATATAA GTCAAATGTC CCCTTCCTCA 
GAACATGAAG TCTGGCAGAC AGTCATCTCT CCAGACCTCA GCCAGGTGAC CCTCTCTCCA 
GAACTCAGCC AGACAAACCT CTCTCCAGAC CTCAGCCACA CGACTCTCTC TCCAGAACTC 
ATTCAGAGAA ACCTTTCCCC AGCCCTCGGT CAGATGCCCA TTTCTCCAGA CCTCAGCCAT 
ACAACCCTTT CTCCAGACCT CAGCCATACA ACCCTTTCTT TAGACCTCAG CCAGACAAAC 
CTCTCTCCAG AACTCAGTCA GACAAACCTT TCTCCAGCCC TCGGTCAGAT GCCCCTTTCT 
CCAGACCTCA GCCATACAAC CCTTTCTCTA GACTTCAGCC AGACAAACCT CTCTCCAGAA 
CTCAGCCATA TGACTCTCTC TCCAGAACTC AGTCAGACAA ACCTTTCCCC AGCCCTCGGT 
CAGATGCCCA TTTCTCCAGA CCTCAGCCAT ACAACCCTTT CTCTAGACTT CAGCCAGACA 
AACCTCTCTC CAGAACTCAG TCAAACAAAC CTTTCCCCAG CCCTCGGTCA GATGCCCCTT 
TCTCCAGACC CCAGCCATAC AACCCTTTCT CTAGACCTCA GCCAGACAAA CCTCTCTCCA 
GAACTCAGTC AGACAAACCT TTCCCCAGAC CTCAGTGAGA TGCCCCTCTT TGCAGATCTC 
AGTCAAATTC CCCTTACCCC AGACCTCGAC CAGATGACAC TTTCTCCAGA CCTTGGTGAG 
ACAGATCTTT CCCCAAACTT TGGTCAGATG TCCCTTTCCC CAGACCTCAG CCAGGTGACT 
CTCTCTCCAG ACATCAGTGA CACCACCCTT CTCCCGGATC TCAGCCAGAT ATCACCTCCT 
CCAGACCTTG ATCAGATATT CTACCCTTCT GAATCTAGTC AGTCATTGCT TCTTCAAGAA 
TTTAATGAGT CTTTTCCTTA TCCAGACCTT GGTCAGATGC CATCTCCTTC ATCTCCTACT 
CTCAATGATA CTTTTCTATC AAAGGAATTT AATCCACTGG TTATAGTGGG CCTCAGTAAA 
GATGGTACAG ATTACATTGA GATCATTCCA AAGGAAGAGG TCCAGAGCAG TGAAGATGAC 
TATGCTGAAA TTGATTATGT GCCCTATGAT GACCCCTACA AAACTGATGT TAGGACAAAC 
ATCAACTCCT CCAGAGATCC TGACAACATT GCAGCATGGT ACCTCCGCAG CAACAATGGA 
AACAGAAGAA ATTATTACAT TGCTGCTGAA GAAATATCCT GGGATTATTC AGAATTTGTA 
CAAAGGGAAA CAGATATTGA AGACTCTGAT GATATTCCAG AAGATACCAC ATATAAGAAA 
GTAGTTTTTC GAAAGTACCT CGACAGCACT TTTACCAAAC GTGATCCTCG AGGGGAGTAT 
GAAGAGCATC TCGGAATTCT TGGTCCTATT ATCAGAGCTG AAGTGGATGA TGTTATCCAA 
GTTCGTTTTA AAAATTTAGC ATCCAGACCG TATTCTCTAC ATGCCCATGG ACTTTCCTAT 
GAAAAATCAT CAGAGGGAAA GACTTATGAA GATGACTCTC CTGAATGGTT TAAGGAAGAT 
AATGCTGTTC AGCCAAATAG CAGTTATACC TACGTATGGC ATGCCACTGA GCGATCAGGG 
CCAGAAAGTC CTGGCTCTGC CTGTCGGGCT TGGGCCTACT ACTCAGCTGT GAACCCAGAA 
AAAGATATTC ACTCAGGCTT GATAGGTCCC CTCCTAATCT GCCAAAAAGG AATACTACAT 
AAGGACAGCA ACATGCCTAT GGACATGAGA GAATTTGTCT TACTATTTAT GACCTTTGAT 
GAAAAGAAGA GCTGGTACTA TGAAAAGAAG TCCCGAAGTT CTTGGAGACT CACATCCTCA 
GAAATGAAAA AATCCCATGA GTTTCACGCC ATTAATGGGA TGATCTACAG CTTGCCTGGC 
CTGAAAATGT ATGAGCAAGA GTGGGTGAGG TTACACCTGC TGAACATAGG CGGCTCCCAA 
GACATTCACG TGGTTCACTT TCACGGCCAG ACCTTGCTGG AAAATGGCAA TAAACAGCAC 
CAGTTAGGGG TCTGGCCCCT TCTGCCTGGT TCATTTAAAA CTCTTGAAAT GAAGGCATCA 
AAACCTGGCT GGTGGCTCCT AAACACAGAG GTTGGAGAAA ACCAGAGAGC AGGGATGCAA 
ACGCCATTTC TTATCATGGA CAGAGACTGT AGGATGCCAA TGGGACTAAG CACTGGTATC 
ATATCTGATT CACAGATCAA GGCTTCAGAG TTTCTGGGTT ACTGGGAGCC CAGATTAGCA 
AGATTAAACA ATGGTGGATC TTATAATGCT TGGAGTGTAG AAAAACTTGC AGCAGAATTT 
GCCTCTAAAC CTTGGATCCA GGTGGACATG CAAAAGGAAG TCATAATCAC AGGGATCCAG 
ACCCAAGGTG CCAAACACTA CCTGAAGTCC TGCTATACCA CAGAGTTCTA TGTAGCTTAC 
AGTTCCAACC AGATCAACTG GCAGATCTTC AAAGGGAACA GCACAAGGAA TGTGATGTAT 
TTTAATGGCA ATTCAGATGC CTCTACAATA AAAGAGAATC AGTTTGACCC ACCTATTGTG 
GCTAGATATA TTAGGATCTC TCCAACTCGA GCCTATAACA GACCTACCCT TCGATTGGAA 
CTGCAAGGTT GTGAGGTAAA TGGATGTTCC ACACCCCTGG GTATGGAAAA TGGAAAGATA 
GAAAACAAGC AAATCACAGC TTCTTCGTTT AAGAAATCTT GGTGGGGAGA TTACTGGGAA 
CCCTTCCGTG CCCGTCTGAA TGCCCAGGGA CGTGTGAATG CCTGGCAAGC CAAGGCAAAC 
AACAATAAGC AGTGGCTAGA AATTGATCTA CTCAAGATCA AGAAGATAAC GGCAATTATA 
ACACAGGGCT GCAAGTCTCT GTCCTCTGAA ATGTATGTAA AGAGCTATAC CATCCACTAC 
AGTGAGCAGG GAGTGGAATG GAAACCATAC AGGCTGAAAT CCTCCATGGT GGACAAGATT 
TTTGAAGGAA ATACTAATAC CAAAGGACAT GTGAAGAACT TTTTCAACCC CCCAATCATT 
TCCAGGTTTA TCCGTGTCAT TCCTAAAACA TGGAATCAAA GTATTGCACT TCGCCTGGAA 
CTCTTTGGCT GTGATATTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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F5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

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