MLKL cDNA ORF clone, Homo sapiens(human)

The following MLKL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MLKL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu16012 NM_152649.4
Latest version!
Homo sapiens mixed lineage kinase domain like pseudokinase (MLKL), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu16015 NM_001142497.3
Latest version!
Homo sapiens mixed lineage kinase domain like pseudokinase (MLKL), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$154.50-$216.30
$309.00
OHu16012 XM_005255834.4
Latest version!
Homo sapiens mixed lineage kinase domain like pseudokinase (MLKL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu77539 XM_011522936.3
Latest version!
Homo sapiens mixed lineage kinase domain like pseudokinase (MLKL), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
View Old Accession Versions >>
OHu77539 XM_011522936.2 Homo sapiens mixed lineage kinase domain-like (MLKL), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu16015 NM_001142497.1 Homo sapiens mixed lineage kinase domain-like (MLKL), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$154.50-$216.30
$309.00
OHu16012 XM_005255834.3 Homo sapiens mixed lineage kinase domain-like (MLKL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu16012 NM_152649.2 Homo sapiens mixed lineage kinase domain-like (MLKL), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu16012 XM_005255834.2 Homo sapiens mixed lineage kinase domain-like (MLKL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu16012 NM_152649.3 Homo sapiens mixed lineage kinase domain like pseudokinase (MLKL), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu16015 NM_001142497.2 Homo sapiens mixed lineage kinase domain like pseudokinase (MLKL), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$154.50-$216.30
$309.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu16012
    Clone ID Related Accession (Same CDS sequence) NM_152649.2 , XM_005255834.4 , XM_005255834.3 , NM_152649.3 , XM_005255834.2 , NM_152649.4
    Accession Version NM_152649.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product mixed lineage kinase domain-like protein isoform 1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTATTTA
    CCACCAAAAT GCATGCAGGA GATCCCGCAA GAGCAAATCA AGGAGATCAA GAAGGAGCAG
    CTTTCAGGAT CCCCGTGGAT TCTGCTAAGG GAAAATGAAG TCAGCACACT TTATAAAGGA
    GAATACCACA GAGCTCCAGT GGCCATAAAA GTATTCAAAA AACTCCAGGC TGGCAGCATT
    GCAATAGTGA GGCAGACTTT CAATAAGGAG ATCAAAACCA TGAAGAAATT CGAATCTCCC
    AACATCCTGC GTATATTTGG GATTTGCATT GATGAAACAG TGACTCCGCC TCAATTCTCC
    ATTGTCATGG AGTACTGTGA ACTCGGGACC CTGAGGGAGC TGTTGGATAG GGAAAAAGAC
    CTCACACTTG GCAAGCGCAT GGTCCTAGTC CTGGGGGCAG CCCGAGGCCT ATACCGGCTA
    CACCATTCAG AAGCACCTGA ACTCCACGGA AAAATCAGAA GCTCAAACTT CCTGGTAACT
    CAAGGCTACC AAGTGAAGCT TGCAGGATTT GAGTTGAGGA AAACACAGAC TTCCATGAGT
    TTGGGAACTA CGAGAGAAAA GACAGACAGA GTCAAATCTA CAGCATATCT CTCACCTCAG
    GAACTGGAAG ATGTATTTTA TCAATATGAT GTAAAGTCTG AAATATACAG CTTTGGAATC
    GTCCTCTGGG AAATCGCCAC TGGAGATATC CCGTTTCAAG GCTGTAATTC TGAGAAGATC
    CGCAAGCTGG TGGCTGTGAA GCGGCAGCAG GAGCCACTGG GTGAAGACTG CCCTTCAGAG
    CTGCGGGAGA TCATTGATGA GTGCCGGGCC CATGATCCCT CTGTGCGGCC CTCTGTGGAT
    GAAATCTTAA AGAAACTCTC CACCTTTTCT AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_689862.1
    Translation

    Target ORF information:

    RefSeq Version NM_152649.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens mixed lineage kinase domain-like (MLKL), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_152649.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTATTTA
    CCACCAAAAT GCATGCAGGA GATCCCGCAA GAGCAAATCA AGGAGATCAA GAAGGAGCAG
    CTTTCAGGAT CCCCGTGGAT TCTGCTAAGG GAAAATGAAG TCAGCACACT TTATAAAGGA
    GAATACCACA GAGCTCCAGT GGCCATAAAA GTATTCAAAA AACTCCAGGC TGGCAGCATT
    GCAATAGTGA GGCAGACTTT CAATAAGGAG ATCAAAACCA TGAAGAAATT CGAATCTCCC
    AACATCCTGC GTATATTTGG GATTTGCATT GATGAAACAG TGACTCCGCC TCAATTCTCC
    ATTGTCATGG AGTACTGTGA ACTCGGGACC CTGAGGGAGC TGTTGGATAG GGAAAAAGAC
    CTCACACTTG GCAAGCGCAT GGTCCTAGTC CTGGGGGCAG CCCGAGGCCT ATACCGGCTA
    CACCATTCAG AAGCACCTGA ACTCCACGGA AAAATCAGAA GCTCAAACTT CCTGGTAACT
    CAAGGCTACC AAGTGAAGCT TGCAGGATTT GAGTTGAGGA AAACACAGAC TTCCATGAGT
    TTGGGAACTA CGAGAGAAAA GACAGACAGA GTCAAATCTA CAGCATATCT CTCACCTCAG
    GAACTGGAAG ATGTATTTTA TCAATATGAT GTAAAGTCTG AAATATACAG CTTTGGAATC
    GTCCTCTGGG AAATCGCCAC TGGAGATATC CCGTTTCAAG GCTGTAATTC TGAGAAGATC
    CGCAAGCTGG TGGCTGTGAA GCGGCAGCAG GAGCCACTGG GTGAAGACTG CCCTTCAGAG
    CTGCGGGAGA TCATTGATGA GTGCCGGGCC CATGATCCCT CTGTGCGGCC CTCTGTGGAT
    GAAATCTTAA AGAAACTCTC CACCTTTTCT AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16012
    Clone ID Related Accession (Same CDS sequence) NM_152649.2 , XM_005255834.4 , XM_005255834.3 , NM_152649.3 , XM_005255834.2 , NM_152649.4
    Accession Version NM_152649.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-31
    Organism Homo sapiens(human)
    Product mixed lineage kinase domain-like protein isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA219811.1, AK091708.1, BC028141.1 and BQ575071.1. On Sep 9, 2015 this sequence version replaced NM_152649.2. Transcript Variant: This variant (1, also known as MLKLshRNA-A) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.129689.1, SRR1660807.179482.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTATTTA
    CCACCAAAAT GCATGCAGGA GATCCCGCAA GAGCAAATCA AGGAGATCAA GAAGGAGCAG
    CTTTCAGGAT CCCCGTGGAT TCTGCTAAGG GAAAATGAAG TCAGCACACT TTATAAAGGA
    GAATACCACA GAGCTCCAGT GGCCATAAAA GTATTCAAAA AACTCCAGGC TGGCAGCATT
    GCAATAGTGA GGCAGACTTT CAATAAGGAG ATCAAAACCA TGAAGAAATT CGAATCTCCC
    AACATCCTGC GTATATTTGG GATTTGCATT GATGAAACAG TGACTCCGCC TCAATTCTCC
    ATTGTCATGG AGTACTGTGA ACTCGGGACC CTGAGGGAGC TGTTGGATAG GGAAAAAGAC
    CTCACACTTG GCAAGCGCAT GGTCCTAGTC CTGGGGGCAG CCCGAGGCCT ATACCGGCTA
    CACCATTCAG AAGCACCTGA ACTCCACGGA AAAATCAGAA GCTCAAACTT CCTGGTAACT
    CAAGGCTACC AAGTGAAGCT TGCAGGATTT GAGTTGAGGA AAACACAGAC TTCCATGAGT
    TTGGGAACTA CGAGAGAAAA GACAGACAGA GTCAAATCTA CAGCATATCT CTCACCTCAG
    GAACTGGAAG ATGTATTTTA TCAATATGAT GTAAAGTCTG AAATATACAG CTTTGGAATC
    GTCCTCTGGG AAATCGCCAC TGGAGATATC CCGTTTCAAG GCTGTAATTC TGAGAAGATC
    CGCAAGCTGG TGGCTGTGAA GCGGCAGCAG GAGCCACTGG GTGAAGACTG CCCTTCAGAG
    CTGCGGGAGA TCATTGATGA GTGCCGGGCC CATGATCCCT CTGTGCGGCC CTCTGTGGAT
    GAAATCTTAA AGAAACTCTC CACCTTTTCT AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_689862.1
    CDS699..2114
    Translation

    Target ORF information:

    RefSeq Version NM_152649.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mixed lineage kinase domain like pseudokinase (MLKL), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_152649.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTATTTA
    CCACCAAAAT GCATGCAGGA GATCCCGCAA GAGCAAATCA AGGAGATCAA GAAGGAGCAG
    CTTTCAGGAT CCCCGTGGAT TCTGCTAAGG GAAAATGAAG TCAGCACACT TTATAAAGGA
    GAATACCACA GAGCTCCAGT GGCCATAAAA GTATTCAAAA AACTCCAGGC TGGCAGCATT
    GCAATAGTGA GGCAGACTTT CAATAAGGAG ATCAAAACCA TGAAGAAATT CGAATCTCCC
    AACATCCTGC GTATATTTGG GATTTGCATT GATGAAACAG TGACTCCGCC TCAATTCTCC
    ATTGTCATGG AGTACTGTGA ACTCGGGACC CTGAGGGAGC TGTTGGATAG GGAAAAAGAC
    CTCACACTTG GCAAGCGCAT GGTCCTAGTC CTGGGGGCAG CCCGAGGCCT ATACCGGCTA
    CACCATTCAG AAGCACCTGA ACTCCACGGA AAAATCAGAA GCTCAAACTT CCTGGTAACT
    CAAGGCTACC AAGTGAAGCT TGCAGGATTT GAGTTGAGGA AAACACAGAC TTCCATGAGT
    TTGGGAACTA CGAGAGAAAA GACAGACAGA GTCAAATCTA CAGCATATCT CTCACCTCAG
    GAACTGGAAG ATGTATTTTA TCAATATGAT GTAAAGTCTG AAATATACAG CTTTGGAATC
    GTCCTCTGGG AAATCGCCAC TGGAGATATC CCGTTTCAAG GCTGTAATTC TGAGAAGATC
    CGCAAGCTGG TGGCTGTGAA GCGGCAGCAG GAGCCACTGG GTGAAGACTG CCCTTCAGAG
    CTGCGGGAGA TCATTGATGA GTGCCGGGCC CATGATCCCT CTGTGCGGCC CTCTGTGGAT
    GAAATCTTAA AGAAACTCTC CACCTTTTCT AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16012
    Clone ID Related Accession (Same CDS sequence) NM_152649.2 , XM_005255834.4 , XM_005255834.3 , NM_152649.3 , XM_005255834.2 , NM_152649.4
    Accession Version NM_152649.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-25
    Organism Homo sapiens(human)
    Product mixed lineage kinase domain-like protein isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK091708.1, BC028141.1 and BQ575071.1. On Nov 22, 2018 this sequence version replaced NM_152649.3. Transcript Variant: This variant (1, also known as MLKLshRNA-A) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.214897.1, AK091708.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000308807.12/ ENSP00000308351.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTATTTA
    CCACCAAAAT GCATGCAGGA GATCCCGCAA GAGCAAATCA AGGAGATCAA GAAGGAGCAG
    CTTTCAGGAT CCCCGTGGAT TCTGCTAAGG GAAAATGAAG TCAGCACACT TTATAAAGGA
    GAATACCACA GAGCTCCAGT GGCCATAAAA GTATTCAAAA AACTCCAGGC TGGCAGCATT
    GCAATAGTGA GGCAGACTTT CAATAAGGAG ATCAAAACCA TGAAGAAATT CGAATCTCCC
    AACATCCTGC GTATATTTGG GATTTGCATT GATGAAACAG TGACTCCGCC TCAATTCTCC
    ATTGTCATGG AGTACTGTGA ACTCGGGACC CTGAGGGAGC TGTTGGATAG GGAAAAAGAC
    CTCACACTTG GCAAGCGCAT GGTCCTAGTC CTGGGGGCAG CCCGAGGCCT ATACCGGCTA
    CACCATTCAG AAGCACCTGA ACTCCACGGA AAAATCAGAA GCTCAAACTT CCTGGTAACT
    CAAGGCTACC AAGTGAAGCT TGCAGGATTT GAGTTGAGGA AAACACAGAC TTCCATGAGT
    TTGGGAACTA CGAGAGAAAA GACAGACAGA GTCAAATCTA CAGCATATCT CTCACCTCAG
    GAACTGGAAG ATGTATTTTA TCAATATGAT GTAAAGTCTG AAATATACAG CTTTGGAATC
    GTCCTCTGGG AAATCGCCAC TGGAGATATC CCGTTTCAAG GCTGTAATTC TGAGAAGATC
    CGCAAGCTGG TGGCTGTGAA GCGGCAGCAG GAGCCACTGG GTGAAGACTG CCCTTCAGAG
    CTGCGGGAGA TCATTGATGA GTGCCGGGCC CATGATCCCT CTGTGCGGCC CTCTGTGGAT
    GAAATCTTAA AGAAACTCTC CACCTTTTCT AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_689862.1
    CDS413..1828
    Translation

    Target ORF information:

    RefSeq Version NM_152649.4
    Organism Homo sapiens(human)
    Definition Homo sapiens mixed lineage kinase domain like pseudokinase (MLKL), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_152649.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTATTTA
    CCACCAAAAT GCATGCAGGA GATCCCGCAA GAGCAAATCA AGGAGATCAA GAAGGAGCAG
    CTTTCAGGAT CCCCGTGGAT TCTGCTAAGG GAAAATGAAG TCAGCACACT TTATAAAGGA
    GAATACCACA GAGCTCCAGT GGCCATAAAA GTATTCAAAA AACTCCAGGC TGGCAGCATT
    GCAATAGTGA GGCAGACTTT CAATAAGGAG ATCAAAACCA TGAAGAAATT CGAATCTCCC
    AACATCCTGC GTATATTTGG GATTTGCATT GATGAAACAG TGACTCCGCC TCAATTCTCC
    ATTGTCATGG AGTACTGTGA ACTCGGGACC CTGAGGGAGC TGTTGGATAG GGAAAAAGAC
    CTCACACTTG GCAAGCGCAT GGTCCTAGTC CTGGGGGCAG CCCGAGGCCT ATACCGGCTA
    CACCATTCAG AAGCACCTGA ACTCCACGGA AAAATCAGAA GCTCAAACTT CCTGGTAACT
    CAAGGCTACC AAGTGAAGCT TGCAGGATTT GAGTTGAGGA AAACACAGAC TTCCATGAGT
    TTGGGAACTA CGAGAGAAAA GACAGACAGA GTCAAATCTA CAGCATATCT CTCACCTCAG
    GAACTGGAAG ATGTATTTTA TCAATATGAT GTAAAGTCTG AAATATACAG CTTTGGAATC
    GTCCTCTGGG AAATCGCCAC TGGAGATATC CCGTTTCAAG GCTGTAATTC TGAGAAGATC
    CGCAAGCTGG TGGCTGTGAA GCGGCAGCAG GAGCCACTGG GTGAAGACTG CCCTTCAGAG
    CTGCGGGAGA TCATTGATGA GTGCCGGGCC CATGATCCCT CTGTGCGGCC CTCTGTGGAT
    GAAATCTTAA AGAAACTCTC CACCTTTTCT AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16015
    Clone ID Related Accession (Same CDS sequence) NM_001142497.2 , NM_001142497.1 , NM_001142497.3
    Accession Version NM_001142497.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-09
    Organism Homo sapiens(human)
    Product mixed lineage kinase domain-like protein isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA219811.1, DB291522.1, BC028141.1 and BQ575071.1. On Sep 9, 2015 this sequence version replaced NM_001142497.1. Transcript Variant: This variant (2, also known as MLKLshRNA-B) lacks several consecutive exons in the mid region and uses an alternate donor splice site at the next exon, which causes a localized frameshift compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter, lacking an internal protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028141.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2162568, SAMEA2467148 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTCTTTG
    GAATCGTCCT CTGGGAAATC GCCACTGGAG ATATCCCGTT TCAAGGTGAA GAATGTGAAG
    ACTGGCTCAG CCAGTGGCTG TAATTCTGAG AAGATCCGCA AGCTGGTGGC TGTGAAGCGG
    CAGCAGGAGC CACTGGGTGA AGACTGCCCT TCAGAGCTGC GGGAGATCAT TGATGAGTGC
    CGGGCCCATG ATCCCTCTGT GCGGCCCTCT GTGGATGAAA TCTTAAAGAA ACTCTCCACC
    TTTTCTAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001135969.1
    CDS699..1490
    Translation

    Target ORF information:

    RefSeq Version NM_001142497.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mixed lineage kinase domain like pseudokinase (MLKL), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001142497.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTCTTTG
    GAATCGTCCT CTGGGAAATC GCCACTGGAG ATATCCCGTT TCAAGGTGAA GAATGTGAAG
    ACTGGCTCAG CCAGTGGCTG TAATTCTGAG AAGATCCGCA AGCTGGTGGC TGTGAAGCGG
    CAGCAGGAGC CACTGGGTGA AGACTGCCCT TCAGAGCTGC GGGAGATCAT TGATGAGTGC
    CGGGCCCATG ATCCCTCTGT GCGGCCCTCT GTGGATGAAA TCTTAAAGAA ACTCTCCACC
    TTTTCTAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16015
    Clone ID Related Accession (Same CDS sequence) NM_001142497.2 , NM_001142497.1 , NM_001142497.3
    Accession Version NM_001142497.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product mixed lineage kinase domain-like protein isoform 2
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTCTTTG
    GAATCGTCCT CTGGGAAATC GCCACTGGAG ATATCCCGTT TCAAGGTGAA GAATGTGAAG
    ACTGGCTCAG CCAGTGGCTG TAATTCTGAG AAGATCCGCA AGCTGGTGGC TGTGAAGCGG
    CAGCAGGAGC CACTGGGTGA AGACTGCCCT TCAGAGCTGC GGGAGATCAT TGATGAGTGC
    CGGGCCCATG ATCCCTCTGT GCGGCCCTCT GTGGATGAAA TCTTAAAGAA ACTCTCCACC
    TTTTCTAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001135969.1
    Translation

    Target ORF information:

    RefSeq Version NM_001142497.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens mixed lineage kinase domain-like (MLKL), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001142497.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTCTTTG
    GAATCGTCCT CTGGGAAATC GCCACTGGAG ATATCCCGTT TCAAGGTGAA GAATGTGAAG
    ACTGGCTCAG CCAGTGGCTG TAATTCTGAG AAGATCCGCA AGCTGGTGGC TGTGAAGCGG
    CAGCAGGAGC CACTGGGTGA AGACTGCCCT TCAGAGCTGC GGGAGATCAT TGATGAGTGC
    CGGGCCCATG ATCCCTCTGT GCGGCCCTCT GTGGATGAAA TCTTAAAGAA ACTCTCCACC
    TTTTCTAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16015
    Clone ID Related Accession (Same CDS sequence) NM_001142497.2 , NM_001142497.1 , NM_001142497.3
    Accession Version NM_001142497.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-25
    Organism Homo sapiens(human)
    Product mixed lineage kinase domain-like protein isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB291522.1, BC028141.1 and BQ575071.1. On May 31, 2019 this sequence version replaced NM_001142497.2. Transcript Variant: This variant (2, also known as MLKLshRNA-B) lacks several consecutive exons in the mid region and uses an alternate donor splice site at the next exon, which causes a localized frameshift compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter, lacking an internal protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028141.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2162568, SAMEA2467148 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTCTTTG
    GAATCGTCCT CTGGGAAATC GCCACTGGAG ATATCCCGTT TCAAGGTGAA GAATGTGAAG
    ACTGGCTCAG CCAGTGGCTG TAATTCTGAG AAGATCCGCA AGCTGGTGGC TGTGAAGCGG
    CAGCAGGAGC CACTGGGTGA AGACTGCCCT TCAGAGCTGC GGGAGATCAT TGATGAGTGC
    CGGGCCCATG ATCCCTCTGT GCGGCCCTCT GTGGATGAAA TCTTAAAGAA ACTCTCCACC
    TTTTCTAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001135969.1
    CDS413..1204
    Translation

    Target ORF information:

    RefSeq Version NM_001142497.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mixed lineage kinase domain like pseudokinase (MLKL), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001142497.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTCTTTG
    GAATCGTCCT CTGGGAAATC GCCACTGGAG ATATCCCGTT TCAAGGTGAA GAATGTGAAG
    ACTGGCTCAG CCAGTGGCTG TAATTCTGAG AAGATCCGCA AGCTGGTGGC TGTGAAGCGG
    CAGCAGGAGC CACTGGGTGA AGACTGCCCT TCAGAGCTGC GGGAGATCAT TGATGAGTGC
    CGGGCCCATG ATCCCTCTGT GCGGCCCTCT GTGGATGAAA TCTTAAAGAA ACTCTCCACC
    TTTTCTAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16012
    Clone ID Related Accession (Same CDS sequence) NM_152649.2 , XM_005255834.4 , XM_005255834.3 , NM_152649.3 , XM_005255834.2 , NM_152649.4
    Accession Version XM_005255834.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mixed lineage kinase domain-like protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005255834.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTATTTA
    CCACCAAAAT GCATGCAGGA GATCCCGCAA GAGCAAATCA AGGAGATCAA GAAGGAGCAG
    CTTTCAGGAT CCCCGTGGAT TCTGCTAAGG GAAAATGAAG TCAGCACACT TTATAAAGGA
    GAATACCACA GAGCTCCAGT GGCCATAAAA GTATTCAAAA AACTCCAGGC TGGCAGCATT
    GCAATAGTGA GGCAGACTTT CAATAAGGAG ATCAAAACCA TGAAGAAATT CGAATCTCCC
    AACATCCTGC GTATATTTGG GATTTGCATT GATGAAACAG TGACTCCGCC TCAATTCTCC
    ATTGTCATGG AGTACTGTGA ACTCGGGACC CTGAGGGAGC TGTTGGATAG GGAAAAAGAC
    CTCACACTTG GCAAGCGCAT GGTCCTAGTC CTGGGGGCAG CCCGAGGCCT ATACCGGCTA
    CACCATTCAG AAGCACCTGA ACTCCACGGA AAAATCAGAA GCTCAAACTT CCTGGTAACT
    CAAGGCTACC AAGTGAAGCT TGCAGGATTT GAGTTGAGGA AAACACAGAC TTCCATGAGT
    TTGGGAACTA CGAGAGAAAA GACAGACAGA GTCAAATCTA CAGCATATCT CTCACCTCAG
    GAACTGGAAG ATGTATTTTA TCAATATGAT GTAAAGTCTG AAATATACAG CTTTGGAATC
    GTCCTCTGGG AAATCGCCAC TGGAGATATC CCGTTTCAAG GCTGTAATTC TGAGAAGATC
    CGCAAGCTGG TGGCTGTGAA GCGGCAGCAG GAGCCACTGG GTGAAGACTG CCCTTCAGAG
    CTGCGGGAGA TCATTGATGA GTGCCGGGCC CATGATCCCT CTGTGCGGCC CTCTGTGGAT
    GAAATCTTAA AGAAACTCTC CACCTTTTCT AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005255891.1
    CDS300..1715
    Translation

    Target ORF information:

    RefSeq Version XM_005255834.4
    Organism Homo sapiens(human)
    Definition Homo sapiens mixed lineage kinase domain like pseudokinase (MLKL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005255834.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTATTTA
    CCACCAAAAT GCATGCAGGA GATCCCGCAA GAGCAAATCA AGGAGATCAA GAAGGAGCAG
    CTTTCAGGAT CCCCGTGGAT TCTGCTAAGG GAAAATGAAG TCAGCACACT TTATAAAGGA
    GAATACCACA GAGCTCCAGT GGCCATAAAA GTATTCAAAA AACTCCAGGC TGGCAGCATT
    GCAATAGTGA GGCAGACTTT CAATAAGGAG ATCAAAACCA TGAAGAAATT CGAATCTCCC
    AACATCCTGC GTATATTTGG GATTTGCATT GATGAAACAG TGACTCCGCC TCAATTCTCC
    ATTGTCATGG AGTACTGTGA ACTCGGGACC CTGAGGGAGC TGTTGGATAG GGAAAAAGAC
    CTCACACTTG GCAAGCGCAT GGTCCTAGTC CTGGGGGCAG CCCGAGGCCT ATACCGGCTA
    CACCATTCAG AAGCACCTGA ACTCCACGGA AAAATCAGAA GCTCAAACTT CCTGGTAACT
    CAAGGCTACC AAGTGAAGCT TGCAGGATTT GAGTTGAGGA AAACACAGAC TTCCATGAGT
    TTGGGAACTA CGAGAGAAAA GACAGACAGA GTCAAATCTA CAGCATATCT CTCACCTCAG
    GAACTGGAAG ATGTATTTTA TCAATATGAT GTAAAGTCTG AAATATACAG CTTTGGAATC
    GTCCTCTGGG AAATCGCCAC TGGAGATATC CCGTTTCAAG GCTGTAATTC TGAGAAGATC
    CGCAAGCTGG TGGCTGTGAA GCGGCAGCAG GAGCCACTGG GTGAAGACTG CCCTTCAGAG
    CTGCGGGAGA TCATTGATGA GTGCCGGGCC CATGATCCCT CTGTGCGGCC CTCTGTGGAT
    GAAATCTTAA AGAAACTCTC CACCTTTTCT AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16012
    Clone ID Related Accession (Same CDS sequence) NM_152649.2 , XM_005255834.4 , XM_005255834.3 , NM_152649.3 , XM_005255834.2 , NM_152649.4
    Accession Version XM_005255834.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mixed lineage kinase domain-like protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005255834.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTATTTA
    CCACCAAAAT GCATGCAGGA GATCCCGCAA GAGCAAATCA AGGAGATCAA GAAGGAGCAG
    CTTTCAGGAT CCCCGTGGAT TCTGCTAAGG GAAAATGAAG TCAGCACACT TTATAAAGGA
    GAATACCACA GAGCTCCAGT GGCCATAAAA GTATTCAAAA AACTCCAGGC TGGCAGCATT
    GCAATAGTGA GGCAGACTTT CAATAAGGAG ATCAAAACCA TGAAGAAATT CGAATCTCCC
    AACATCCTGC GTATATTTGG GATTTGCATT GATGAAACAG TGACTCCGCC TCAATTCTCC
    ATTGTCATGG AGTACTGTGA ACTCGGGACC CTGAGGGAGC TGTTGGATAG GGAAAAAGAC
    CTCACACTTG GCAAGCGCAT GGTCCTAGTC CTGGGGGCAG CCCGAGGCCT ATACCGGCTA
    CACCATTCAG AAGCACCTGA ACTCCACGGA AAAATCAGAA GCTCAAACTT CCTGGTAACT
    CAAGGCTACC AAGTGAAGCT TGCAGGATTT GAGTTGAGGA AAACACAGAC TTCCATGAGT
    TTGGGAACTA CGAGAGAAAA GACAGACAGA GTCAAATCTA CAGCATATCT CTCACCTCAG
    GAACTGGAAG ATGTATTTTA TCAATATGAT GTAAAGTCTG AAATATACAG CTTTGGAATC
    GTCCTCTGGG AAATCGCCAC TGGAGATATC CCGTTTCAAG GCTGTAATTC TGAGAAGATC
    CGCAAGCTGG TGGCTGTGAA GCGGCAGCAG GAGCCACTGG GTGAAGACTG CCCTTCAGAG
    CTGCGGGAGA TCATTGATGA GTGCCGGGCC CATGATCCCT CTGTGCGGCC CTCTGTGGAT
    GAAATCTTAA AGAAACTCTC CACCTTTTCT AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005255891.1
    CDS598..2013
    Translation

    Target ORF information:

    RefSeq Version XM_005255834.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mixed lineage kinase domain-like (MLKL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005255834.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTATTTA
    CCACCAAAAT GCATGCAGGA GATCCCGCAA GAGCAAATCA AGGAGATCAA GAAGGAGCAG
    CTTTCAGGAT CCCCGTGGAT TCTGCTAAGG GAAAATGAAG TCAGCACACT TTATAAAGGA
    GAATACCACA GAGCTCCAGT GGCCATAAAA GTATTCAAAA AACTCCAGGC TGGCAGCATT
    GCAATAGTGA GGCAGACTTT CAATAAGGAG ATCAAAACCA TGAAGAAATT CGAATCTCCC
    AACATCCTGC GTATATTTGG GATTTGCATT GATGAAACAG TGACTCCGCC TCAATTCTCC
    ATTGTCATGG AGTACTGTGA ACTCGGGACC CTGAGGGAGC TGTTGGATAG GGAAAAAGAC
    CTCACACTTG GCAAGCGCAT GGTCCTAGTC CTGGGGGCAG CCCGAGGCCT ATACCGGCTA
    CACCATTCAG AAGCACCTGA ACTCCACGGA AAAATCAGAA GCTCAAACTT CCTGGTAACT
    CAAGGCTACC AAGTGAAGCT TGCAGGATTT GAGTTGAGGA AAACACAGAC TTCCATGAGT
    TTGGGAACTA CGAGAGAAAA GACAGACAGA GTCAAATCTA CAGCATATCT CTCACCTCAG
    GAACTGGAAG ATGTATTTTA TCAATATGAT GTAAAGTCTG AAATATACAG CTTTGGAATC
    GTCCTCTGGG AAATCGCCAC TGGAGATATC CCGTTTCAAG GCTGTAATTC TGAGAAGATC
    CGCAAGCTGG TGGCTGTGAA GCGGCAGCAG GAGCCACTGG GTGAAGACTG CCCTTCAGAG
    CTGCGGGAGA TCATTGATGA GTGCCGGGCC CATGATCCCT CTGTGCGGCC CTCTGTGGAT
    GAAATCTTAA AGAAACTCTC CACCTTTTCT AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16012
    Clone ID Related Accession (Same CDS sequence) NM_152649.2 , XM_005255834.4 , XM_005255834.3 , NM_152649.3 , XM_005255834.2 , NM_152649.4
    Accession Version XM_005255834.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product mixed lineage kinase domain-like protein isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTATTTA
    CCACCAAAAT GCATGCAGGA GATCCCGCAA GAGCAAATCA AGGAGATCAA GAAGGAGCAG
    CTTTCAGGAT CCCCGTGGAT TCTGCTAAGG GAAAATGAAG TCAGCACACT TTATAAAGGA
    GAATACCACA GAGCTCCAGT GGCCATAAAA GTATTCAAAA AACTCCAGGC TGGCAGCATT
    GCAATAGTGA GGCAGACTTT CAATAAGGAG ATCAAAACCA TGAAGAAATT CGAATCTCCC
    AACATCCTGC GTATATTTGG GATTTGCATT GATGAAACAG TGACTCCGCC TCAATTCTCC
    ATTGTCATGG AGTACTGTGA ACTCGGGACC CTGAGGGAGC TGTTGGATAG GGAAAAAGAC
    CTCACACTTG GCAAGCGCAT GGTCCTAGTC CTGGGGGCAG CCCGAGGCCT ATACCGGCTA
    CACCATTCAG AAGCACCTGA ACTCCACGGA AAAATCAGAA GCTCAAACTT CCTGGTAACT
    CAAGGCTACC AAGTGAAGCT TGCAGGATTT GAGTTGAGGA AAACACAGAC TTCCATGAGT
    TTGGGAACTA CGAGAGAAAA GACAGACAGA GTCAAATCTA CAGCATATCT CTCACCTCAG
    GAACTGGAAG ATGTATTTTA TCAATATGAT GTAAAGTCTG AAATATACAG CTTTGGAATC
    GTCCTCTGGG AAATCGCCAC TGGAGATATC CCGTTTCAAG GCTGTAATTC TGAGAAGATC
    CGCAAGCTGG TGGCTGTGAA GCGGCAGCAG GAGCCACTGG GTGAAGACTG CCCTTCAGAG
    CTGCGGGAGA TCATTGATGA GTGCCGGGCC CATGATCCCT CTGTGCGGCC CTCTGTGGAT
    GAAATCTTAA AGAAACTCTC CACCTTTTCT AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005255891.1
    Translation

    Target ORF information:

    RefSeq Version XM_005255834.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens mixed lineage kinase domain-like (MLKL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005255834.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTATTTA
    CCACCAAAAT GCATGCAGGA GATCCCGCAA GAGCAAATCA AGGAGATCAA GAAGGAGCAG
    CTTTCAGGAT CCCCGTGGAT TCTGCTAAGG GAAAATGAAG TCAGCACACT TTATAAAGGA
    GAATACCACA GAGCTCCAGT GGCCATAAAA GTATTCAAAA AACTCCAGGC TGGCAGCATT
    GCAATAGTGA GGCAGACTTT CAATAAGGAG ATCAAAACCA TGAAGAAATT CGAATCTCCC
    AACATCCTGC GTATATTTGG GATTTGCATT GATGAAACAG TGACTCCGCC TCAATTCTCC
    ATTGTCATGG AGTACTGTGA ACTCGGGACC CTGAGGGAGC TGTTGGATAG GGAAAAAGAC
    CTCACACTTG GCAAGCGCAT GGTCCTAGTC CTGGGGGCAG CCCGAGGCCT ATACCGGCTA
    CACCATTCAG AAGCACCTGA ACTCCACGGA AAAATCAGAA GCTCAAACTT CCTGGTAACT
    CAAGGCTACC AAGTGAAGCT TGCAGGATTT GAGTTGAGGA AAACACAGAC TTCCATGAGT
    TTGGGAACTA CGAGAGAAAA GACAGACAGA GTCAAATCTA CAGCATATCT CTCACCTCAG
    GAACTGGAAG ATGTATTTTA TCAATATGAT GTAAAGTCTG AAATATACAG CTTTGGAATC
    GTCCTCTGGG AAATCGCCAC TGGAGATATC CCGTTTCAAG GCTGTAATTC TGAGAAGATC
    CGCAAGCTGG TGGCTGTGAA GCGGCAGCAG GAGCCACTGG GTGAAGACTG CCCTTCAGAG
    CTGCGGGAGA TCATTGATGA GTGCCGGGCC CATGATCCCT CTGTGCGGCC CTCTGTGGAT
    GAAATCTTAA AGAAACTCTC CACCTTTTCT AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77539
    Clone ID Related Accession (Same CDS sequence) XM_011522936.3 , XM_011522936.2
    Accession Version XM_011522936.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mixed lineage kinase domain-like protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011522936.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTATTTA
    CCACCAAAAT GCATGCAGGA GATCCCGCAA GAGCAAATCA AGGAGATCAA GAAGGAGCAG
    CTTTCAGGAT CCCCGTGGAT TCTGCTAAGG GAAAATGAAG TCAGCACACT TTATAAAGGA
    GAATACCACA GAGCTCCAGT GGCCATAAAA GTATTCAAAA AACTCCAGGC TGGCAGCATT
    GCAATAGTGA GGCAGACTTT CAATAAGGAG ATCAAAACCA TGAAGAAATT CGAATCTCCC
    AACATCCTGC GTATATTTGG GATTTGCATT GATGAAACAG GCTACACCAT TCAGAAGCAC
    CTGAACTCCA CGGAAAAATC AGAAGCTCAA ACTTCCTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011521238.1
    CDS413..1294
    Translation

    Target ORF information:

    RefSeq Version XM_011522936.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mixed lineage kinase domain like pseudokinase (MLKL), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011522936.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTATTTA
    CCACCAAAAT GCATGCAGGA GATCCCGCAA GAGCAAATCA AGGAGATCAA GAAGGAGCAG
    CTTTCAGGAT CCCCGTGGAT TCTGCTAAGG GAAAATGAAG TCAGCACACT TTATAAAGGA
    GAATACCACA GAGCTCCAGT GGCCATAAAA GTATTCAAAA AACTCCAGGC TGGCAGCATT
    GCAATAGTGA GGCAGACTTT CAATAAGGAG ATCAAAACCA TGAAGAAATT CGAATCTCCC
    AACATCCTGC GTATATTTGG GATTTGCATT GATGAAACAG GCTACACCAT TCAGAAGCAC
    CTGAACTCCA CGGAAAAATC AGAAGCTCAA ACTTCCTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77539
    Clone ID Related Accession (Same CDS sequence) XM_011522936.3 , XM_011522936.2
    Accession Version XM_011522936.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mixed lineage kinase domain-like protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522936.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTATTTA
    CCACCAAAAT GCATGCAGGA GATCCCGCAA GAGCAAATCA AGGAGATCAA GAAGGAGCAG
    CTTTCAGGAT CCCCGTGGAT TCTGCTAAGG GAAAATGAAG TCAGCACACT TTATAAAGGA
    GAATACCACA GAGCTCCAGT GGCCATAAAA GTATTCAAAA AACTCCAGGC TGGCAGCATT
    GCAATAGTGA GGCAGACTTT CAATAAGGAG ATCAAAACCA TGAAGAAATT CGAATCTCCC
    AACATCCTGC GTATATTTGG GATTTGCATT GATGAAACAG GCTACACCAT TCAGAAGCAC
    CTGAACTCCA CGGAAAAATC AGAAGCTCAA ACTTCCTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011521238.1
    CDS738..1619
    Translation

    Target ORF information:

    RefSeq Version XM_011522936.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mixed lineage kinase domain-like (MLKL), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011522936.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAAAATT TGAAGCATAT TATCACCCTT GGCCAGGTCA TCCACAAACG GTGTGAAGAG 
    ATGAAATACT GCAAGAAACA GTGCCGGCGC CTGGGCCACC GCGTCCTCGG CCTGATCAAG
    CCTCTGGAGA TGCTCCAGGA CCAAGGAAAG AGGAGCGTGC CCTCTGAGAA GTTAACCACA
    GCCATGAACC GCTTCAAGGC TGCCCTGGAG GAGGCTAATG GGGAGATAGA AAAGTTCAGC
    AATAGATCCA ATATCTGCAG GTTTCTAACA GCAAGCCAGG ACAAAATACT CTTCAAGGAC
    GTGAACAGGA AGCTGAGTGA TGTCTGGAAG GAGCTCTCGC TGTTACTTCA GGTTGAGCAA
    CGCATGCCTG TTTCACCCAT AAGCCAAGGA GCGTCCTGGG CACAGGAAGA TCAGCAGGAT
    GCAGACGAAG ACAGGCGAGC TTTCCAGATG CTAAGAAGAG ATAATGAAAA AATAGAAGCT
    TCACTGAGAC GATTAGAAAT CAACATGAAA GAAATCAAGG AAACTTTGAG GCAGTATTTA
    CCACCAAAAT GCATGCAGGA GATCCCGCAA GAGCAAATCA AGGAGATCAA GAAGGAGCAG
    CTTTCAGGAT CCCCGTGGAT TCTGCTAAGG GAAAATGAAG TCAGCACACT TTATAAAGGA
    GAATACCACA GAGCTCCAGT GGCCATAAAA GTATTCAAAA AACTCCAGGC TGGCAGCATT
    GCAATAGTGA GGCAGACTTT CAATAAGGAG ATCAAAACCA TGAAGAAATT CGAATCTCCC
    AACATCCTGC GTATATTTGG GATTTGCATT GATGAAACAG GCTACACCAT TCAGAAGCAC
    CTGAACTCCA CGGAAAAATC AGAAGCTCAA ACTTCCTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.