ACE cDNA ORF clone, Homo sapiens(human)

The following ACE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu14342 NM_001178057.2
Latest version!
Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $489.30
$699.00
OHu14691 NM_152830.3
Latest version!
Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $517.30
$739.00
OHu57207 XM_006721737.3
Latest version!
Homo sapiens angiotensin I converting enzyme (ACE), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu20567 NM_000789.4
Latest version!
Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $923.30
$1319.00
View Old Accession Versions >>
OHu14342 NM_001178057.1 Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $489.30
$699.00
OHu20567 NM_000789.3 Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $923.30
$1319.00
OHu14691 NM_152830.2 Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $517.30
$739.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu14342
    Clone ID Related Accession (Same CDS sequence) NM_001178057.2 , NM_001178057.1
    Accession Version NM_001178057.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product angiotensin-converting enzyme isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM908222.1, M26657.1, AB208971.1, AK301988.1 and AC113554.9. On Jun 1, 2019 this sequence version replaced NM_001178057.1. Transcript Variant: This variant (3) lacks multiple 5' exons and an in-frame exon in the 3' region, but has an alternate 5' exon, compared to variant 1. The resulting isoform (3) is shorter, and has a distinct N-terminus and lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK301988.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGGCCAGG GTTGGGCTAC TGCAGGACTT CCCAGCCTCC TCTTCCTGCT GCTCTGCTAC 
    GGGCACCCTC TGCTGGTCCC CAGCCAGGAG GCATCCCAAC AGGTGACAGT CACCCATGGG
    ACAAGCAGCC AGGCAACAAC CAGCAGCCAG ACAACCACCC ACCAGGCGAC GGCCCACCAG
    ACATCAGCCC AGAGCCCAAA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG
    GAATATGACC GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC
    AACACCAACA TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC
    AACCACACCC TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC
    ACCACTATCA AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC
    CAGGAGCTGG AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC
    ACTGTGTGCC ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG
    GCCACGTCCC GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG
    GGGAGAGCCA TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG
    CTCAATGGCT ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG
    GAGCAAGACC TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC
    TACGTGCGCC GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC
    ATTCCTGCTC ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG
    GTGGTGCCCT TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC
    TGGACGCCCA GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG
    CCCGTGCCTC CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG
    GTGGTCTGCC ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG
    TGCACCACCG TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG
    TATTTCATGC AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC
    CATGAGGCCA TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT
    CTCAACCTGC TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG
    ATGGCCCTTG ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG
    AGGGTATTTG ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG
    CTGAAGTACC AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG
    GCCAAGTTCC ACATTCCTTC TAGCGTGCCT TACATCAGGA CCGCCATGAA GCTGGGCTTC
    AGTAGGCCGT GGCCGGAAGC CATGCAGCTG ATCACGGGCC AGCCCAACAT GAGCGCCTCG
    GCCATGTTGA GCTACTTCAA GCCGCTGCTG GACTGGCTCC GCACGGAGAA CGAGCTGCAT
    GGGGAGAAGC TGGGCTGGCC GCAGTACAAC TGGACGCCGA ACTCCGCTCG CTCAGAAGGG
    CCCCTCCCAG ACAGCGGCCG CGTCAGCTTC CTGGGCCTGG ACCTGGATGC GCAGCAGGCC
    CGCGTGGGCC AGTGGCTGCT GCTCTTCCTG GGCATCGCCC TGCTGGTAGC CACCCTGGGC
    CTCAGCCAGC GGCTCTTCAG CATCCGCCAC CGCAGCCTCC ACCGGCACTC CCACGGGCCC
    CAGTTCGGCT CCGAGGTGGA GCTGAGACAC TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171528.1
    CDS23..2098
    Translation

    Target ORF information:

    RefSeq Version NM_001178057.2
    Organism Homo sapiens(human)
    Definition Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001178057.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGGCCAGG GTTGGGCTAC TGCAGGACTT CCCAGCCTCC TCTTCCTGCT GCTCTGCTAC 
    GGGCACCCTC TGCTGGTCCC CAGCCAGGAG GCATCCCAAC AGGTGACAGT CACCCATGGG
    ACAAGCAGCC AGGCAACAAC CAGCAGCCAG ACAACCACCC ACCAGGCGAC GGCCCACCAG
    ACATCAGCCC AGAGCCCAAA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG
    GAATATGACC GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC
    AACACCAACA TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC
    AACCACACCC TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC
    ACCACTATCA AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC
    CAGGAGCTGG AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC
    ACTGTGTGCC ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG
    GCCACGTCCC GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG
    GGGAGAGCCA TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG
    CTCAATGGCT ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG
    GAGCAAGACC TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC
    TACGTGCGCC GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC
    ATTCCTGCTC ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG
    GTGGTGCCCT TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC
    TGGACGCCCA GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG
    CCCGTGCCTC CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG
    GTGGTCTGCC ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG
    TGCACCACCG TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG
    TATTTCATGC AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC
    CATGAGGCCA TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT
    CTCAACCTGC TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG
    ATGGCCCTTG ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG
    AGGGTATTTG ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG
    CTGAAGTACC AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG
    GCCAAGTTCC ACATTCCTTC TAGCGTGCCT TACATCAGGA CCGCCATGAA GCTGGGCTTC
    AGTAGGCCGT GGCCGGAAGC CATGCAGCTG ATCACGGGCC AGCCCAACAT GAGCGCCTCG
    GCCATGTTGA GCTACTTCAA GCCGCTGCTG GACTGGCTCC GCACGGAGAA CGAGCTGCAT
    GGGGAGAAGC TGGGCTGGCC GCAGTACAAC TGGACGCCGA ACTCCGCTCG CTCAGAAGGG
    CCCCTCCCAG ACAGCGGCCG CGTCAGCTTC CTGGGCCTGG ACCTGGATGC GCAGCAGGCC
    CGCGTGGGCC AGTGGCTGCT GCTCTTCCTG GGCATCGCCC TGCTGGTAGC CACCCTGGGC
    CTCAGCCAGC GGCTCTTCAG CATCCGCCAC CGCAGCCTCC ACCGGCACTC CCACGGGCCC
    CAGTTCGGCT CCGAGGTGGA GCTGAGACAC TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14342
    Clone ID Related Accession (Same CDS sequence) NM_001178057.2 , NM_001178057.1
    Accession Version NM_001178057.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product angiotensin-converting enzyme isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM908222.1, M26657.1, AB208971.1, AK301988.1 and AC113554.9. Transcript Variant: This variant (3) lacks multiple 5' exons and an in-frame exon in the 3' region, but has an alternate 5' exon, compared to variant 1. The resulting isoform (3) is shorter, and has a distinct N-terminus and lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK301988.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGGCCAGG GTTGGGCTAC TGCAGGACTT CCCAGCCTCC TCTTCCTGCT GCTCTGCTAC 
    GGGCACCCTC TGCTGGTCCC CAGCCAGGAG GCATCCCAAC AGGTGACAGT CACCCATGGG
    ACAAGCAGCC AGGCAACAAC CAGCAGCCAG ACAACCACCC ACCAGGCGAC GGCCCACCAG
    ACATCAGCCC AGAGCCCAAA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG
    GAATATGACC GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC
    AACACCAACA TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC
    AACCACACCC TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC
    ACCACTATCA AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC
    CAGGAGCTGG AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC
    ACTGTGTGCC ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG
    GCCACGTCCC GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG
    GGGAGAGCCA TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG
    CTCAATGGCT ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG
    GAGCAAGACC TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC
    TACGTGCGCC GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC
    ATTCCTGCTC ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG
    GTGGTGCCCT TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC
    TGGACGCCCA GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG
    CCCGTGCCTC CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG
    GTGGTCTGCC ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG
    TGCACCACCG TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG
    TATTTCATGC AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC
    CATGAGGCCA TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT
    CTCAACCTGC TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG
    ATGGCCCTTG ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG
    AGGGTATTTG ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG
    CTGAAGTACC AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG
    GCCAAGTTCC ACATTCCTTC TAGCGTGCCT TACATCAGGA CCGCCATGAA GCTGGGCTTC
    AGTAGGCCGT GGCCGGAAGC CATGCAGCTG ATCACGGGCC AGCCCAACAT GAGCGCCTCG
    GCCATGTTGA GCTACTTCAA GCCGCTGCTG GACTGGCTCC GCACGGAGAA CGAGCTGCAT
    GGGGAGAAGC TGGGCTGGCC GCAGTACAAC TGGACGCCGA ACTCCGCTCG CTCAGAAGGG
    CCCCTCCCAG ACAGCGGCCG CGTCAGCTTC CTGGGCCTGG ACCTGGATGC GCAGCAGGCC
    CGCGTGGGCC AGTGGCTGCT GCTCTTCCTG GGCATCGCCC TGCTGGTAGC CACCCTGGGC
    CTCAGCCAGC GGCTCTTCAG CATCCGCCAC CGCAGCCTCC ACCGGCACTC CCACGGGCCC
    CAGTTCGGCT CCGAGGTGGA GCTGAGACAC TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171528.1
    CDS52..2127
    Translation

    Target ORF information:

    RefSeq Version NM_001178057.1
    Organism Homo sapiens(human)
    Definition Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001178057.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGGCCAGG GTTGGGCTAC TGCAGGACTT CCCAGCCTCC TCTTCCTGCT GCTCTGCTAC 
    GGGCACCCTC TGCTGGTCCC CAGCCAGGAG GCATCCCAAC AGGTGACAGT CACCCATGGG
    ACAAGCAGCC AGGCAACAAC CAGCAGCCAG ACAACCACCC ACCAGGCGAC GGCCCACCAG
    ACATCAGCCC AGAGCCCAAA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG
    GAATATGACC GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC
    AACACCAACA TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC
    AACCACACCC TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC
    ACCACTATCA AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC
    CAGGAGCTGG AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC
    ACTGTGTGCC ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG
    GCCACGTCCC GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG
    GGGAGAGCCA TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG
    CTCAATGGCT ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG
    GAGCAAGACC TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC
    TACGTGCGCC GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC
    ATTCCTGCTC ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG
    GTGGTGCCCT TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC
    TGGACGCCCA GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG
    CCCGTGCCTC CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG
    GTGGTCTGCC ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG
    TGCACCACCG TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG
    TATTTCATGC AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC
    CATGAGGCCA TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT
    CTCAACCTGC TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG
    ATGGCCCTTG ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG
    AGGGTATTTG ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG
    CTGAAGTACC AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG
    GCCAAGTTCC ACATTCCTTC TAGCGTGCCT TACATCAGGA CCGCCATGAA GCTGGGCTTC
    AGTAGGCCGT GGCCGGAAGC CATGCAGCTG ATCACGGGCC AGCCCAACAT GAGCGCCTCG
    GCCATGTTGA GCTACTTCAA GCCGCTGCTG GACTGGCTCC GCACGGAGAA CGAGCTGCAT
    GGGGAGAAGC TGGGCTGGCC GCAGTACAAC TGGACGCCGA ACTCCGCTCG CTCAGAAGGG
    CCCCTCCCAG ACAGCGGCCG CGTCAGCTTC CTGGGCCTGG ACCTGGATGC GCAGCAGGCC
    CGCGTGGGCC AGTGGCTGCT GCTCTTCCTG GGCATCGCCC TGCTGGTAGC CACCCTGGGC
    CTCAGCCAGC GGCTCTTCAG CATCCGCCAC CGCAGCCTCC ACCGGCACTC CCACGGGCCC
    CAGTTCGGCT CCGAGGTGGA GCTGAGACAC TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14691
    Clone ID Related Accession (Same CDS sequence) NM_152830.2 , NM_152830.3
    Accession Version NM_152830.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product angiotensin-converting enzyme isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM908222.1, M26657.1, AB208971.1 and AC113554.9. On Sep 25, 2010 this sequence version replaced NM_152830.1. Transcript Variant: This variant (2), also known as the testicular form, lacks multiple 5' exons, but has an alternate 5' exon, compared to variant 1. The resulting isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X16295.1, M26657.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGCCAGG GTTGGGCTAC TGCAGGACTT CCCAGCCTCC TCTTCCTGCT GCTCTGCTAC 
    GGGCACCCTC TGCTGGTCCC CAGCCAGGAG GCATCCCAAC AGGTGACAGT CACCCATGGG
    ACAAGCAGCC AGGCAACAAC CAGCAGCCAG ACAACCACCC ACCAGGCGAC GGCCCACCAG
    ACATCAGCCC AGAGCCCAAA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG
    GAATATGACC GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC
    AACACCAACA TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC
    AACCACACCC TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC
    ACCACTATCA AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC
    CAGGAGCTGG AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC
    ACTGTGTGCC ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG
    GCCACGTCCC GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG
    GGGAGAGCCA TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG
    CTCAATGGCT ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG
    GAGCAAGACC TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC
    TACGTGCGCC GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC
    ATTCCTGCTC ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG
    GTGGTGCCCT TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC
    TGGACGCCCA GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG
    CCCGTGCCTC CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG
    GTGGTCTGCC ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG
    TGCACCACCG TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG
    TATTTCATGC AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC
    CATGAGGCCA TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT
    CTCAACCTGC TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG
    ATGGCCCTTG ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG
    AGGGTATTTG ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG
    CTGAAGTACC AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG
    GCCAAGTTCC ACATTCCTTC TAGCGTGCCT TACATCAGGT ACTTTGTCAG CTTCATCATC
    CAGTTCCAGT TCCACGAGGC ACTGTGCCAG GCAGCTGGCC ACACGGGCCC CCTGCACAAG
    TGTGACATCT ACCAGTCCAA GGAGGCCGGG CAGCGCCTGG CGACCGCCAT GAAGCTGGGC
    TTCAGTAGGC CGTGGCCGGA AGCCATGCAG CTGATCACGG GCCAGCCCAA CATGAGCGCC
    TCGGCCATGT TGAGCTACTT CAAGCCGCTG CTGGACTGGC TCCGCACGGA GAACGAGCTG
    CATGGGGAGA AGCTGGGCTG GCCGCAGTAC AACTGGACGC CGAACTCCGC TCGCTCAGAA
    GGGCCCCTCC CAGACAGCGG CCGCGTCAGC TTCCTGGGCC TGGACCTGGA TGCGCAGCAG
    GCCCGCGTGG GCCAGTGGCT GCTGCTCTTC CTGGGCATCG CCCTGCTGGT AGCCACCCTG
    GGCCTCAGCC AGCGGCTCTT CAGCATCCGC CACCGCAGCC TCCACCGGCA CTCCCACGGG
    CCCCAGTTCG GCTCCGAGGT GGAGCTGAGA CACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_690043.1
    CDS52..2250
    Translation

    Target ORF information:

    RefSeq Version NM_152830.2
    Organism Homo sapiens(human)
    Definition Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_152830.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGCCAGG GTTGGGCTAC TGCAGGACTT CCCAGCCTCC TCTTCCTGCT GCTCTGCTAC 
    GGGCACCCTC TGCTGGTCCC CAGCCAGGAG GCATCCCAAC AGGTGACAGT CACCCATGGG
    ACAAGCAGCC AGGCAACAAC CAGCAGCCAG ACAACCACCC ACCAGGCGAC GGCCCACCAG
    ACATCAGCCC AGAGCCCAAA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG
    GAATATGACC GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC
    AACACCAACA TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC
    AACCACACCC TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC
    ACCACTATCA AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC
    CAGGAGCTGG AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC
    ACTGTGTGCC ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG
    GCCACGTCCC GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG
    GGGAGAGCCA TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG
    CTCAATGGCT ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG
    GAGCAAGACC TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC
    TACGTGCGCC GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC
    ATTCCTGCTC ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG
    GTGGTGCCCT TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC
    TGGACGCCCA GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG
    CCCGTGCCTC CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG
    GTGGTCTGCC ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG
    TGCACCACCG TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG
    TATTTCATGC AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC
    CATGAGGCCA TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT
    CTCAACCTGC TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG
    ATGGCCCTTG ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG
    AGGGTATTTG ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG
    CTGAAGTACC AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG
    GCCAAGTTCC ACATTCCTTC TAGCGTGCCT TACATCAGGT ACTTTGTCAG CTTCATCATC
    CAGTTCCAGT TCCACGAGGC ACTGTGCCAG GCAGCTGGCC ACACGGGCCC CCTGCACAAG
    TGTGACATCT ACCAGTCCAA GGAGGCCGGG CAGCGCCTGG CGACCGCCAT GAAGCTGGGC
    TTCAGTAGGC CGTGGCCGGA AGCCATGCAG CTGATCACGG GCCAGCCCAA CATGAGCGCC
    TCGGCCATGT TGAGCTACTT CAAGCCGCTG CTGGACTGGC TCCGCACGGA GAACGAGCTG
    CATGGGGAGA AGCTGGGCTG GCCGCAGTAC AACTGGACGC CGAACTCCGC TCGCTCAGAA
    GGGCCCCTCC CAGACAGCGG CCGCGTCAGC TTCCTGGGCC TGGACCTGGA TGCGCAGCAG
    GCCCGCGTGG GCCAGTGGCT GCTGCTCTTC CTGGGCATCG CCCTGCTGGT AGCCACCCTG
    GGCCTCAGCC AGCGGCTCTT CAGCATCCGC CACCGCAGCC TCCACCGGCA CTCCCACGGG
    CCCCAGTTCG GCTCCGAGGT GGAGCTGAGA CACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14691
    Clone ID Related Accession (Same CDS sequence) NM_152830.2 , NM_152830.3
    Accession Version NM_152830.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product angiotensin-converting enzyme isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM908222.1, M26657.1, AB208971.1 and AC113554.9. On Jun 1, 2019 this sequence version replaced NM_152830.2. Transcript Variant: This variant (2), also known as the testicular form, lacks multiple 5' exons, but has an alternate 5' exon, compared to variant 1. The resulting isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M26657.1, X16295.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGCCAGG GTTGGGCTAC TGCAGGACTT CCCAGCCTCC TCTTCCTGCT GCTCTGCTAC 
    GGGCACCCTC TGCTGGTCCC CAGCCAGGAG GCATCCCAAC AGGTGACAGT CACCCATGGG
    ACAAGCAGCC AGGCAACAAC CAGCAGCCAG ACAACCACCC ACCAGGCGAC GGCCCACCAG
    ACATCAGCCC AGAGCCCAAA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG
    GAATATGACC GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC
    AACACCAACA TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC
    AACCACACCC TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC
    ACCACTATCA AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC
    CAGGAGCTGG AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC
    ACTGTGTGCC ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG
    GCCACGTCCC GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG
    GGGAGAGCCA TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG
    CTCAATGGCT ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG
    GAGCAAGACC TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC
    TACGTGCGCC GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC
    ATTCCTGCTC ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG
    GTGGTGCCCT TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC
    TGGACGCCCA GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG
    CCCGTGCCTC CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG
    GTGGTCTGCC ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG
    TGCACCACCG TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG
    TATTTCATGC AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC
    CATGAGGCCA TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT
    CTCAACCTGC TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG
    ATGGCCCTTG ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG
    AGGGTATTTG ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG
    CTGAAGTACC AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG
    GCCAAGTTCC ACATTCCTTC TAGCGTGCCT TACATCAGGT ACTTTGTCAG CTTCATCATC
    CAGTTCCAGT TCCACGAGGC ACTGTGCCAG GCAGCTGGCC ACACGGGCCC CCTGCACAAG
    TGTGACATCT ACCAGTCCAA GGAGGCCGGG CAGCGCCTGG CGACCGCCAT GAAGCTGGGC
    TTCAGTAGGC CGTGGCCGGA AGCCATGCAG CTGATCACGG GCCAGCCCAA CATGAGCGCC
    TCGGCCATGT TGAGCTACTT CAAGCCGCTG CTGGACTGGC TCCGCACGGA GAACGAGCTG
    CATGGGGAGA AGCTGGGCTG GCCGCAGTAC AACTGGACGC CGAACTCCGC TCGCTCAGAA
    GGGCCCCTCC CAGACAGCGG CCGCGTCAGC TTCCTGGGCC TGGACCTGGA TGCGCAGCAG
    GCCCGCGTGG GCCAGTGGCT GCTGCTCTTC CTGGGCATCG CCCTGCTGGT AGCCACCCTG
    GGCCTCAGCC AGCGGCTCTT CAGCATCCGC CACCGCAGCC TCCACCGGCA CTCCCACGGG
    CCCCAGTTCG GCTCCGAGGT GGAGCTGAGA CACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_690043.1
    CDS23..2221
    Translation

    Target ORF information:

    RefSeq Version NM_152830.3
    Organism Homo sapiens(human)
    Definition Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_152830.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGCCAGG GTTGGGCTAC TGCAGGACTT CCCAGCCTCC TCTTCCTGCT GCTCTGCTAC 
    GGGCACCCTC TGCTGGTCCC CAGCCAGGAG GCATCCCAAC AGGTGACAGT CACCCATGGG
    ACAAGCAGCC AGGCAACAAC CAGCAGCCAG ACAACCACCC ACCAGGCGAC GGCCCACCAG
    ACATCAGCCC AGAGCCCAAA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG
    GAATATGACC GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC
    AACACCAACA TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC
    AACCACACCC TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC
    ACCACTATCA AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC
    CAGGAGCTGG AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC
    ACTGTGTGCC ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG
    GCCACGTCCC GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG
    GGGAGAGCCA TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG
    CTCAATGGCT ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG
    GAGCAAGACC TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC
    TACGTGCGCC GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC
    ATTCCTGCTC ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG
    GTGGTGCCCT TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC
    TGGACGCCCA GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG
    CCCGTGCCTC CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG
    GTGGTCTGCC ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG
    TGCACCACCG TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG
    TATTTCATGC AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC
    CATGAGGCCA TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT
    CTCAACCTGC TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG
    ATGGCCCTTG ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG
    AGGGTATTTG ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG
    CTGAAGTACC AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG
    GCCAAGTTCC ACATTCCTTC TAGCGTGCCT TACATCAGGT ACTTTGTCAG CTTCATCATC
    CAGTTCCAGT TCCACGAGGC ACTGTGCCAG GCAGCTGGCC ACACGGGCCC CCTGCACAAG
    TGTGACATCT ACCAGTCCAA GGAGGCCGGG CAGCGCCTGG CGACCGCCAT GAAGCTGGGC
    TTCAGTAGGC CGTGGCCGGA AGCCATGCAG CTGATCACGG GCCAGCCCAA CATGAGCGCC
    TCGGCCATGT TGAGCTACTT CAAGCCGCTG CTGGACTGGC TCCGCACGGA GAACGAGCTG
    CATGGGGAGA AGCTGGGCTG GCCGCAGTAC AACTGGACGC CGAACTCCGC TCGCTCAGAA
    GGGCCCCTCC CAGACAGCGG CCGCGTCAGC TTCCTGGGCC TGGACCTGGA TGCGCAGCAG
    GCCCGCGTGG GCCAGTGGCT GCTGCTCTTC CTGGGCATCG CCCTGCTGGT AGCCACCCTG
    GGCCTCAGCC AGCGGCTCTT CAGCATCCGC CACCGCAGCC TCCACCGGCA CTCCCACGGG
    CCCCAGTTCG GCTCCGAGGT GGAGCTGAGA CACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57207
    Clone ID Related Accession (Same CDS sequence) XM_006721737.3
    Accession Version XM_006721737.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2259bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product angiotensin-converting enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006721737.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTTTCC CCCAGCATCC TAGAGAGGGT GTGCTCAGAC CTGAGGGCCC CTCCCCTTCC 
    AGAGGAAGCC AGACACAAGG CTCTGTGAGG TCACACTGCG GGCTCCGCTC TTATTGGCCA
    GGGGACGGTA GCTGCAGGAC TCTGCTCTCC TGCGGCCATG GGCCAGGGTT GGGCTACTGC
    AGGACTTCCC AGCCTCCTCT TCCTGCTGCT CTGCTACGGG CACCCTCTGC TGGTCCCCAG
    CCAGGAGGCA TCCCAACAGA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG
    GAATATGACC GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC
    AACACCAACA TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC
    AACCACACCC TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC
    ACCACTATCA AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC
    CAGGAGCTGG AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC
    ACTGTGTGCC ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG
    GCCACGTCCC GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG
    GGGAGAGCCA TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG
    CTCAATGGCT ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG
    GAGCAAGACC TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC
    TACGTGCGCC GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC
    ATTCCTGCTC ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG
    GTGGTGCCCT TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC
    TGGACGCCCA GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG
    CCCGTGCCTC CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG
    GTGGTCTGCC ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG
    TGCACCACCG TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG
    TATTTCATGC AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC
    CATGAGGCCA TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT
    CTCAACCTGC TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG
    ATGGCCCTTG ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG
    AGGGTATTTG ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG
    CTGAAGTACC AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG
    GCCAAGTTCC ACATTCCTTC TAGCGTGCCT TACATCAGGT ACTTTGTCAG CTTCATCATC
    CAGTTCCAGT TCCACGAGGC ACTGTGCCAG GCAGCTGGCC ACACGGGCCC CCTGCACAAG
    TGTGACATCT ACCAGTCCAA GGAGGCCGGG CAGCGCCTGG CGACCGCCAT GAAGCTGGGC
    TTCAGTAGGC CGTGGCCGGA AGCCATGCAG CTGATCACGG GCCAGCCCAA CATGAGCGCC
    TCGGCCATGT TGAGCTACTT CAAGCCGCTG CTGGACTGGC TCCGCACGGA GAACGAGCTG
    CATGGGGAGA AGCTGGGCTG GCCGCAGTAC AACTGGACGC CGAACTCCGC TCGCTCAGAA
    GGGCCCCTCC CAGACAGCGG CCGCGTCAGC TTCCTGGGCC TGGACCTGGA TGCGCAGCAG
    GCCCGCGTGG GCCAGTGGCT GCTGCTCTTC CTGGGCATCG CCCTGCTGGT AGCCACCCTG
    GGCCTCAGCC AGCGGCTCTT CAGCATCCGC CACCGCAGCC TCCACCGGCA CTCCCACGGG
    CCCCAGTTCG GCTCCGAGGT GGAGCTGAGA CACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006721800.2
    CDS105..2363
    Translation

    Target ORF information:

    RefSeq Version XM_006721737.3
    Organism Homo sapiens(human)
    Definition Homo sapiens angiotensin I converting enzyme (ACE), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006721737.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTTTCC CCCAGCATCC TAGAGAGGGT GTGCTCAGAC CTGAGGGCCC CTCCCCTTCC 
    AGAGGAAGCC AGACACAAGG CTCTGTGAGG TCACACTGCG GGCTCCGCTC TTATTGGCCA
    GGGGACGGTA GCTGCAGGAC TCTGCTCTCC TGCGGCCATG GGCCAGGGTT GGGCTACTGC
    AGGACTTCCC AGCCTCCTCT TCCTGCTGCT CTGCTACGGG CACCCTCTGC TGGTCCCCAG
    CCAGGAGGCA TCCCAACAGA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG
    GAATATGACC GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC
    AACACCAACA TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC
    AACCACACCC TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC
    ACCACTATCA AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC
    CAGGAGCTGG AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC
    ACTGTGTGCC ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG
    GCCACGTCCC GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG
    GGGAGAGCCA TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG
    CTCAATGGCT ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG
    GAGCAAGACC TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC
    TACGTGCGCC GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC
    ATTCCTGCTC ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG
    GTGGTGCCCT TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC
    TGGACGCCCA GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG
    CCCGTGCCTC CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG
    GTGGTCTGCC ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG
    TGCACCACCG TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG
    TATTTCATGC AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC
    CATGAGGCCA TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT
    CTCAACCTGC TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG
    ATGGCCCTTG ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG
    AGGGTATTTG ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG
    CTGAAGTACC AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG
    GCCAAGTTCC ACATTCCTTC TAGCGTGCCT TACATCAGGT ACTTTGTCAG CTTCATCATC
    CAGTTCCAGT TCCACGAGGC ACTGTGCCAG GCAGCTGGCC ACACGGGCCC CCTGCACAAG
    TGTGACATCT ACCAGTCCAA GGAGGCCGGG CAGCGCCTGG CGACCGCCAT GAAGCTGGGC
    TTCAGTAGGC CGTGGCCGGA AGCCATGCAG CTGATCACGG GCCAGCCCAA CATGAGCGCC
    TCGGCCATGT TGAGCTACTT CAAGCCGCTG CTGGACTGGC TCCGCACGGA GAACGAGCTG
    CATGGGGAGA AGCTGGGCTG GCCGCAGTAC AACTGGACGC CGAACTCCGC TCGCTCAGAA
    GGGCCCCTCC CAGACAGCGG CCGCGTCAGC TTCCTGGGCC TGGACCTGGA TGCGCAGCAG
    GCCCGCGTGG GCCAGTGGCT GCTGCTCTTC CTGGGCATCG CCCTGCTGGT AGCCACCCTG
    GGCCTCAGCC AGCGGCTCTT CAGCATCCGC CACCGCAGCC TCCACCGGCA CTCCCACGGG
    CCCCAGTTCG GCTCCGAGGT GGAGCTGAGA CACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20567
    Clone ID Related Accession (Same CDS sequence) NM_000789.3 , NM_000789.4
    Accession Version NM_000789.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-31
    Organism Homo sapiens(human)
    Product angiotensin-converting enzyme isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK296566.1, AB208971.1 and AC113554.9. This sequence is a reference standard in the RefSeqGene project. On Sep 25, 2010 this sequence version replaced NM_000789.2. Transcript Variant: This variant (1), also known as the endothelial or somatic form, represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J04144.1, AB208971.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGGGCCG CCTCGGGCCG CCGGGGGCCG GGGCTGCTGC TGCCGCTGCC GCTGCTGTTG 
    CTGCTGCCGC CGCAGCCCGC CCTGGCGTTG GACCCCGGGC TGCAGCCCGG CAACTTTTCT
    GCTGACGAGG CCGGGGCGCA GCTCTTCGCG CAGAGCTACA ACTCCAGCGC CGAACAGGTG
    CTGTTCCAGA GCGTGGCCGC CAGCTGGGCG CACGACACCA ACATCACCGC GGAGAATGCA
    AGGCGCCAGG AGGAAGCAGC CCTGCTCAGC CAGGAGTTTG CGGAGGCCTG GGGCCAGAAG
    GCCAAGGAGC TGTATGAACC GATCTGGCAG AACTTCACGG ACCCGCAGCT GCGCAGGATC
    ATCGGAGCTG TGCGCACCCT GGGCTCTGCC AACCTGCCCC TGGCTAAGCG GCAGCAGTAC
    AACGCCCTGC TAAGCAACAT GAGCAGGATC TACTCCACCG CCAAGGTCTG CCTCCCCAAC
    AAGACTGCCA CCTGCTGGTC CCTGGACCCA GATCTCACCA ACATCCTGGC TTCCTCGCGA
    AGCTACGCCA TGCTCCTGTT TGCCTGGGAG GGCTGGCACA ACGCTGCGGG CATCCCGCTG
    AAACCGCTGT ACGAGGATTT CACTGCCCTC AGCAATGAAG CCTACAAGCA GGACGGCTTC
    ACAGACACGG GGGCCTACTG GCGCTCCTGG TACAACTCCC CCACCTTCGA GGACGATCTG
    GAACACCTCT ACCAACAGCT AGAGCCCCTC TACCTGAACC TCCATGCCTT CGTCCGCCGC
    GCACTGCATC GCCGATACGG AGACAGATAC ATCAACCTCA GGGGACCCAT CCCTGCTCAT
    CTGCTGGGAG ACATGTGGGC CCAGAGCTGG GAAAACATCT ACGACATGGT GGTGCCTTTC
    CCAGACAAGC CCAACCTCGA TGTCACCAGT ACTATGCTGC AGCAGGGCTG GAACGCCACG
    CACATGTTCC GGGTGGCAGA GGAGTTCTTC ACCTCCCTGG AGCTCTCCCC CATGCCTCCC
    GAGTTCTGGG AAGGGTCGAT GCTGGAGAAG CCGGCCGACG GGCGGGAAGT GGTGTGCCAC
    GCCTCGGCTT GGGACTTCTA CAACAGGAAA GACTTCAGGA TCAAGCAGTG CACACGGGTC
    ACGATGGACC AGCTCTCCAC AGTGCACCAT GAGATGGGCC ATATACAGTA CTACCTGCAG
    TACAAGGATC TGCCCGTCTC CCTGCGTCGG GGGGCCAACC CCGGCTTCCA TGAGGCCATT
    GGGGACGTGC TGGCGCTCTC GGTCTCCACT CCTGAACATC TGCACAAAAT CGGCCTGCTG
    GACCGTGTCA CCAATGACAC GGAAAGTGAC ATCAATTACT TGCTAAAAAT GGCACTGGAA
    AAAATTGCCT TCCTGCCCTT TGGCTACTTG GTGGACCAGT GGCGCTGGGG GGTCTTTAGT
    GGGCGTACCC CCCCTTCCCG CTACAACTTC GACTGGTGGT ATCTTCGAAC CAAGTATCAG
    GGGATCTGTC CTCCTGTTAC CCGAAACGAA ACCCACTTTG ATGCTGGAGC TAAGTTTCAT
    GTTCCAAATG TGACACCATA CATCAGGTAC TTTGTGAGTT TTGTCCTGCA GTTCCAGTTC
    CATGAAGCCC TGTGCAAGGA GGCAGGCTAT GAGGGCCCAC TGCACCAGTG TGACATCTAC
    CGGTCCACCA AGGCAGGGGC CAAGCTCCGG AAGGTGCTGC AGGCTGGCTC CTCCAGGCCC
    TGGCAGGAGG TGCTGAAGGA CATGGTCGGC TTAGATGCCC TGGATGCCCA GCCGCTGCTC
    AAGTACTTCC AGCCAGTCAC CCAGTGGCTG CAGGAGCAGA ACCAGCAGAA CGGCGAGGTC
    CTGGGCTGGC CCGAGTACCA GTGGCACCCG CCGTTGCCTG ACAACTACCC GGAGGGCATA
    GACCTGGTGA CTGATGAGGC TGAGGCCAGC AAGTTTGTGG AGGAATATGA CCGGACATCC
    CAGGTGGTGT GGAACGAGTA TGCCGAGGCC AACTGGAACT ACAACACCAA CATCACCACA
    GAGACCAGCA AGATTCTGCT GCAGAAGAAC ATGCAAATAG CCAACCACAC CCTGAAGTAC
    GGCACCCAGG CCAGGAAGTT TGATGTGAAC CAGTTGCAGA ACACCACTAT CAAGCGGATC
    ATAAAGAAGG TTCAGGACCT AGAACGGGCA GCACTGCCTG CCCAGGAGCT GGAGGAGTAC
    AACAAGATCC TGTTGGATAT GGAAACCACC TACAGCGTGG CCACTGTGTG CCACCCGAAT
    GGCAGCTGCC TGCAGCTCGA GCCAGATCTG ACGAATGTGA TGGCCACGTC CCGGAAATAT
    GAAGACCTGT TATGGGCATG GGAGGGCTGG CGAGACAAGG CGGGGAGAGC CATCCTCCAG
    TTTTACCCGA AATACGTGGA ACTCATCAAC CAGGCTGCCC GGCTCAATGG CTATGTAGAT
    GCAGGGGACT CGTGGAGGTC TATGTACGAG ACACCATCCC TGGAGCAAGA CCTGGAGCGG
    CTCTTCCAGG AGCTGCAGCC ACTCTACCTC AACCTGCATG CCTACGTGCG CCGGGCCCTG
    CACCGTCACT ACGGGGCCCA GCACATCAAC CTGGAGGGGC CCATTCCTGC TCACCTGCTG
    GGGAACATGT GGGCGCAGAC CTGGTCCAAC ATCTATGACT TGGTGGTGCC CTTCCCTTCA
    GCCCCCTCGA TGGACACCAC AGAGGCTATG CTAAAGCAGG GCTGGACGCC CAGGAGGATG
    TTTAAGGAGG CTGATGATTT CTTCACCTCC CTGGGGCTGC TGCCCGTGCC TCCTGAGTTC
    TGGAACAAGT CGATGCTGGA GAAGCCAACC GACGGGCGGG AGGTGGTCTG CCACGCCTCG
    GCCTGGGACT TCTACAACGG CAAGGACTTC CGGATCAAGC AGTGCACCAC CGTGAACTTG
    GAGGACCTGG TGGTGGCCCA CCACGAAATG GGCCACATCC AGTATTTCAT GCAGTACAAA
    GACTTACCTG TGGCCTTGAG GGAGGGTGCC AACCCCGGCT TCCATGAGGC CATTGGGGAC
    GTGCTAGCCC TCTCAGTGTC TACGCCCAAG CACCTGCACA GTCTCAACCT GCTGAGCAGT
    GAGGGTGGCA GCGACGAGCA TGACATCAAC TTTCTGATGA AGATGGCCCT TGACAAGATC
    GCCTTTATCC CCTTCAGCTA CCTCGTCGAT CAGTGGCGCT GGAGGGTATT TGATGGAAGC
    ATCACCAAGG AGAACTATAA CCAGGAGTGG TGGAGCCTCA GGCTGAAGTA CCAGGGCCTC
    TGCCCCCCAG TGCCCAGGAC TCAAGGTGAC TTTGACCCAG GGGCCAAGTT CCACATTCCT
    TCTAGCGTGC CTTACATCAG GTACTTTGTC AGCTTCATCA TCCAGTTCCA GTTCCACGAG
    GCACTGTGCC AGGCAGCTGG CCACACGGGC CCCCTGCACA AGTGTGACAT CTACCAGTCC
    AAGGAGGCCG GGCAGCGCCT GGCGACCGCC ATGAAGCTGG GCTTCAGTAG GCCGTGGCCG
    GAAGCCATGC AGCTGATCAC GGGCCAGCCC AACATGAGCG CCTCGGCCAT GTTGAGCTAC
    TTCAAGCCGC TGCTGGACTG GCTCCGCACG GAGAACGAGC TGCATGGGGA GAAGCTGGGC
    TGGCCGCAGT ACAACTGGAC GCCGAACTCC GCTCGCTCAG AAGGGCCCCT CCCAGACAGC
    GGCCGCGTCA GCTTCCTGGG CCTGGACCTG GATGCGCAGC AGGCCCGCGT GGGCCAGTGG
    CTGCTGCTCT TCCTGGGCAT CGCCCTGCTG GTAGCCACCC TGGGCCTCAG CCAGCGGCTC
    TTCAGCATCC GCCACCGCAG CCTCCACCGG CACTCCCACG GGCCCCAGTT CGGCTCCGAG
    GTGGAGCTGA GACACTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000780.1
    CDS35..3955
    Translation

    Target ORF information:

    RefSeq Version NM_000789.3
    Organism Homo sapiens(human)
    Definition Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000789.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGGGCCG CCTCGGGCCG CCGGGGGCCG GGGCTGCTGC TGCCGCTGCC GCTGCTGTTG 
    CTGCTGCCGC CGCAGCCCGC CCTGGCGTTG GACCCCGGGC TGCAGCCCGG CAACTTTTCT
    GCTGACGAGG CCGGGGCGCA GCTCTTCGCG CAGAGCTACA ACTCCAGCGC CGAACAGGTG
    CTGTTCCAGA GCGTGGCCGC CAGCTGGGCG CACGACACCA ACATCACCGC GGAGAATGCA
    AGGCGCCAGG AGGAAGCAGC CCTGCTCAGC CAGGAGTTTG CGGAGGCCTG GGGCCAGAAG
    GCCAAGGAGC TGTATGAACC GATCTGGCAG AACTTCACGG ACCCGCAGCT GCGCAGGATC
    ATCGGAGCTG TGCGCACCCT GGGCTCTGCC AACCTGCCCC TGGCTAAGCG GCAGCAGTAC
    AACGCCCTGC TAAGCAACAT GAGCAGGATC TACTCCACCG CCAAGGTCTG CCTCCCCAAC
    AAGACTGCCA CCTGCTGGTC CCTGGACCCA GATCTCACCA ACATCCTGGC TTCCTCGCGA
    AGCTACGCCA TGCTCCTGTT TGCCTGGGAG GGCTGGCACA ACGCTGCGGG CATCCCGCTG
    AAACCGCTGT ACGAGGATTT CACTGCCCTC AGCAATGAAG CCTACAAGCA GGACGGCTTC
    ACAGACACGG GGGCCTACTG GCGCTCCTGG TACAACTCCC CCACCTTCGA GGACGATCTG
    GAACACCTCT ACCAACAGCT AGAGCCCCTC TACCTGAACC TCCATGCCTT CGTCCGCCGC
    GCACTGCATC GCCGATACGG AGACAGATAC ATCAACCTCA GGGGACCCAT CCCTGCTCAT
    CTGCTGGGAG ACATGTGGGC CCAGAGCTGG GAAAACATCT ACGACATGGT GGTGCCTTTC
    CCAGACAAGC CCAACCTCGA TGTCACCAGT ACTATGCTGC AGCAGGGCTG GAACGCCACG
    CACATGTTCC GGGTGGCAGA GGAGTTCTTC ACCTCCCTGG AGCTCTCCCC CATGCCTCCC
    GAGTTCTGGG AAGGGTCGAT GCTGGAGAAG CCGGCCGACG GGCGGGAAGT GGTGTGCCAC
    GCCTCGGCTT GGGACTTCTA CAACAGGAAA GACTTCAGGA TCAAGCAGTG CACACGGGTC
    ACGATGGACC AGCTCTCCAC AGTGCACCAT GAGATGGGCC ATATACAGTA CTACCTGCAG
    TACAAGGATC TGCCCGTCTC CCTGCGTCGG GGGGCCAACC CCGGCTTCCA TGAGGCCATT
    GGGGACGTGC TGGCGCTCTC GGTCTCCACT CCTGAACATC TGCACAAAAT CGGCCTGCTG
    GACCGTGTCA CCAATGACAC GGAAAGTGAC ATCAATTACT TGCTAAAAAT GGCACTGGAA
    AAAATTGCCT TCCTGCCCTT TGGCTACTTG GTGGACCAGT GGCGCTGGGG GGTCTTTAGT
    GGGCGTACCC CCCCTTCCCG CTACAACTTC GACTGGTGGT ATCTTCGAAC CAAGTATCAG
    GGGATCTGTC CTCCTGTTAC CCGAAACGAA ACCCACTTTG ATGCTGGAGC TAAGTTTCAT
    GTTCCAAATG TGACACCATA CATCAGGTAC TTTGTGAGTT TTGTCCTGCA GTTCCAGTTC
    CATGAAGCCC TGTGCAAGGA GGCAGGCTAT GAGGGCCCAC TGCACCAGTG TGACATCTAC
    CGGTCCACCA AGGCAGGGGC CAAGCTCCGG AAGGTGCTGC AGGCTGGCTC CTCCAGGCCC
    TGGCAGGAGG TGCTGAAGGA CATGGTCGGC TTAGATGCCC TGGATGCCCA GCCGCTGCTC
    AAGTACTTCC AGCCAGTCAC CCAGTGGCTG CAGGAGCAGA ACCAGCAGAA CGGCGAGGTC
    CTGGGCTGGC CCGAGTACCA GTGGCACCCG CCGTTGCCTG ACAACTACCC GGAGGGCATA
    GACCTGGTGA CTGATGAGGC TGAGGCCAGC AAGTTTGTGG AGGAATATGA CCGGACATCC
    CAGGTGGTGT GGAACGAGTA TGCCGAGGCC AACTGGAACT ACAACACCAA CATCACCACA
    GAGACCAGCA AGATTCTGCT GCAGAAGAAC ATGCAAATAG CCAACCACAC CCTGAAGTAC
    GGCACCCAGG CCAGGAAGTT TGATGTGAAC CAGTTGCAGA ACACCACTAT CAAGCGGATC
    ATAAAGAAGG TTCAGGACCT AGAACGGGCA GCACTGCCTG CCCAGGAGCT GGAGGAGTAC
    AACAAGATCC TGTTGGATAT GGAAACCACC TACAGCGTGG CCACTGTGTG CCACCCGAAT
    GGCAGCTGCC TGCAGCTCGA GCCAGATCTG ACGAATGTGA TGGCCACGTC CCGGAAATAT
    GAAGACCTGT TATGGGCATG GGAGGGCTGG CGAGACAAGG CGGGGAGAGC CATCCTCCAG
    TTTTACCCGA AATACGTGGA ACTCATCAAC CAGGCTGCCC GGCTCAATGG CTATGTAGAT
    GCAGGGGACT CGTGGAGGTC TATGTACGAG ACACCATCCC TGGAGCAAGA CCTGGAGCGG
    CTCTTCCAGG AGCTGCAGCC ACTCTACCTC AACCTGCATG CCTACGTGCG CCGGGCCCTG
    CACCGTCACT ACGGGGCCCA GCACATCAAC CTGGAGGGGC CCATTCCTGC TCACCTGCTG
    GGGAACATGT GGGCGCAGAC CTGGTCCAAC ATCTATGACT TGGTGGTGCC CTTCCCTTCA
    GCCCCCTCGA TGGACACCAC AGAGGCTATG CTAAAGCAGG GCTGGACGCC CAGGAGGATG
    TTTAAGGAGG CTGATGATTT CTTCACCTCC CTGGGGCTGC TGCCCGTGCC TCCTGAGTTC
    TGGAACAAGT CGATGCTGGA GAAGCCAACC GACGGGCGGG AGGTGGTCTG CCACGCCTCG
    GCCTGGGACT TCTACAACGG CAAGGACTTC CGGATCAAGC AGTGCACCAC CGTGAACTTG
    GAGGACCTGG TGGTGGCCCA CCACGAAATG GGCCACATCC AGTATTTCAT GCAGTACAAA
    GACTTACCTG TGGCCTTGAG GGAGGGTGCC AACCCCGGCT TCCATGAGGC CATTGGGGAC
    GTGCTAGCCC TCTCAGTGTC TACGCCCAAG CACCTGCACA GTCTCAACCT GCTGAGCAGT
    GAGGGTGGCA GCGACGAGCA TGACATCAAC TTTCTGATGA AGATGGCCCT TGACAAGATC
    GCCTTTATCC CCTTCAGCTA CCTCGTCGAT CAGTGGCGCT GGAGGGTATT TGATGGAAGC
    ATCACCAAGG AGAACTATAA CCAGGAGTGG TGGAGCCTCA GGCTGAAGTA CCAGGGCCTC
    TGCCCCCCAG TGCCCAGGAC TCAAGGTGAC TTTGACCCAG GGGCCAAGTT CCACATTCCT
    TCTAGCGTGC CTTACATCAG GTACTTTGTC AGCTTCATCA TCCAGTTCCA GTTCCACGAG
    GCACTGTGCC AGGCAGCTGG CCACACGGGC CCCCTGCACA AGTGTGACAT CTACCAGTCC
    AAGGAGGCCG GGCAGCGCCT GGCGACCGCC ATGAAGCTGG GCTTCAGTAG GCCGTGGCCG
    GAAGCCATGC AGCTGATCAC GGGCCAGCCC AACATGAGCG CCTCGGCCAT GTTGAGCTAC
    TTCAAGCCGC TGCTGGACTG GCTCCGCACG GAGAACGAGC TGCATGGGGA GAAGCTGGGC
    TGGCCGCAGT ACAACTGGAC GCCGAACTCC GCTCGCTCAG AAGGGCCCCT CCCAGACAGC
    GGCCGCGTCA GCTTCCTGGG CCTGGACCTG GATGCGCAGC AGGCCCGCGT GGGCCAGTGG
    CTGCTGCTCT TCCTGGGCAT CGCCCTGCTG GTAGCCACCC TGGGCCTCAG CCAGCGGCTC
    TTCAGCATCC GCCACCGCAG CCTCCACCGG CACTCCCACG GGCCCCAGTT CGGCTCCGAG
    GTGGAGCTGA GACACTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20567
    Clone ID Related Accession (Same CDS sequence) NM_000789.3 , NM_000789.4
    Accession Version NM_000789.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product angiotensin-converting enzyme isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK296566.1, AB208971.1 and AC113554.9. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000789.3. Transcript Variant: This variant (1), also known as the endothelial or somatic form, represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J04144.1, AB208971.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000290866.10/ ENSP00000290866.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGGGCCG CCTCGGGCCG CCGGGGGCCG GGGCTGCTGC TGCCGCTGCC GCTGCTGTTG 
    CTGCTGCCGC CGCAGCCCGC CCTGGCGTTG GACCCCGGGC TGCAGCCCGG CAACTTTTCT
    GCTGACGAGG CCGGGGCGCA GCTCTTCGCG CAGAGCTACA ACTCCAGCGC CGAACAGGTG
    CTGTTCCAGA GCGTGGCCGC CAGCTGGGCG CACGACACCA ACATCACCGC GGAGAATGCA
    AGGCGCCAGG AGGAAGCAGC CCTGCTCAGC CAGGAGTTTG CGGAGGCCTG GGGCCAGAAG
    GCCAAGGAGC TGTATGAACC GATCTGGCAG AACTTCACGG ACCCGCAGCT GCGCAGGATC
    ATCGGAGCTG TGCGCACCCT GGGCTCTGCC AACCTGCCCC TGGCTAAGCG GCAGCAGTAC
    AACGCCCTGC TAAGCAACAT GAGCAGGATC TACTCCACCG CCAAGGTCTG CCTCCCCAAC
    AAGACTGCCA CCTGCTGGTC CCTGGACCCA GATCTCACCA ACATCCTGGC TTCCTCGCGA
    AGCTACGCCA TGCTCCTGTT TGCCTGGGAG GGCTGGCACA ACGCTGCGGG CATCCCGCTG
    AAACCGCTGT ACGAGGATTT CACTGCCCTC AGCAATGAAG CCTACAAGCA GGACGGCTTC
    ACAGACACGG GGGCCTACTG GCGCTCCTGG TACAACTCCC CCACCTTCGA GGACGATCTG
    GAACACCTCT ACCAACAGCT AGAGCCCCTC TACCTGAACC TCCATGCCTT CGTCCGCCGC
    GCACTGCATC GCCGATACGG AGACAGATAC ATCAACCTCA GGGGACCCAT CCCTGCTCAT
    CTGCTGGGAG ACATGTGGGC CCAGAGCTGG GAAAACATCT ACGACATGGT GGTGCCTTTC
    CCAGACAAGC CCAACCTCGA TGTCACCAGT ACTATGCTGC AGCAGGGCTG GAACGCCACG
    CACATGTTCC GGGTGGCAGA GGAGTTCTTC ACCTCCCTGG AGCTCTCCCC CATGCCTCCC
    GAGTTCTGGG AAGGGTCGAT GCTGGAGAAG CCGGCCGACG GGCGGGAAGT GGTGTGCCAC
    GCCTCGGCTT GGGACTTCTA CAACAGGAAA GACTTCAGGA TCAAGCAGTG CACACGGGTC
    ACGATGGACC AGCTCTCCAC AGTGCACCAT GAGATGGGCC ATATACAGTA CTACCTGCAG
    TACAAGGATC TGCCCGTCTC CCTGCGTCGG GGGGCCAACC CCGGCTTCCA TGAGGCCATT
    GGGGACGTGC TGGCGCTCTC GGTCTCCACT CCTGAACATC TGCACAAAAT CGGCCTGCTG
    GACCGTGTCA CCAATGACAC GGAAAGTGAC ATCAATTACT TGCTAAAAAT GGCACTGGAA
    AAAATTGCCT TCCTGCCCTT TGGCTACTTG GTGGACCAGT GGCGCTGGGG GGTCTTTAGT
    GGGCGTACCC CCCCTTCCCG CTACAACTTC GACTGGTGGT ATCTTCGAAC CAAGTATCAG
    GGGATCTGTC CTCCTGTTAC CCGAAACGAA ACCCACTTTG ATGCTGGAGC TAAGTTTCAT
    GTTCCAAATG TGACACCATA CATCAGGTAC TTTGTGAGTT TTGTCCTGCA GTTCCAGTTC
    CATGAAGCCC TGTGCAAGGA GGCAGGCTAT GAGGGCCCAC TGCACCAGTG TGACATCTAC
    CGGTCCACCA AGGCAGGGGC CAAGCTCCGG AAGGTGCTGC AGGCTGGCTC CTCCAGGCCC
    TGGCAGGAGG TGCTGAAGGA CATGGTCGGC TTAGATGCCC TGGATGCCCA GCCGCTGCTC
    AAGTACTTCC AGCCAGTCAC CCAGTGGCTG CAGGAGCAGA ACCAGCAGAA CGGCGAGGTC
    CTGGGCTGGC CCGAGTACCA GTGGCACCCG CCGTTGCCTG ACAACTACCC GGAGGGCATA
    GACCTGGTGA CTGATGAGGC TGAGGCCAGC AAGTTTGTGG AGGAATATGA CCGGACATCC
    CAGGTGGTGT GGAACGAGTA TGCCGAGGCC AACTGGAACT ACAACACCAA CATCACCACA
    GAGACCAGCA AGATTCTGCT GCAGAAGAAC ATGCAAATAG CCAACCACAC CCTGAAGTAC
    GGCACCCAGG CCAGGAAGTT TGATGTGAAC CAGTTGCAGA ACACCACTAT CAAGCGGATC
    ATAAAGAAGG TTCAGGACCT AGAACGGGCA GCACTGCCTG CCCAGGAGCT GGAGGAGTAC
    AACAAGATCC TGTTGGATAT GGAAACCACC TACAGCGTGG CCACTGTGTG CCACCCGAAT
    GGCAGCTGCC TGCAGCTCGA GCCAGATCTG ACGAATGTGA TGGCCACGTC CCGGAAATAT
    GAAGACCTGT TATGGGCATG GGAGGGCTGG CGAGACAAGG CGGGGAGAGC CATCCTCCAG
    TTTTACCCGA AATACGTGGA ACTCATCAAC CAGGCTGCCC GGCTCAATGG CTATGTAGAT
    GCAGGGGACT CGTGGAGGTC TATGTACGAG ACACCATCCC TGGAGCAAGA CCTGGAGCGG
    CTCTTCCAGG AGCTGCAGCC ACTCTACCTC AACCTGCATG CCTACGTGCG CCGGGCCCTG
    CACCGTCACT ACGGGGCCCA GCACATCAAC CTGGAGGGGC CCATTCCTGC TCACCTGCTG
    GGGAACATGT GGGCGCAGAC CTGGTCCAAC ATCTATGACT TGGTGGTGCC CTTCCCTTCA
    GCCCCCTCGA TGGACACCAC AGAGGCTATG CTAAAGCAGG GCTGGACGCC CAGGAGGATG
    TTTAAGGAGG CTGATGATTT CTTCACCTCC CTGGGGCTGC TGCCCGTGCC TCCTGAGTTC
    TGGAACAAGT CGATGCTGGA GAAGCCAACC GACGGGCGGG AGGTGGTCTG CCACGCCTCG
    GCCTGGGACT TCTACAACGG CAAGGACTTC CGGATCAAGC AGTGCACCAC CGTGAACTTG
    GAGGACCTGG TGGTGGCCCA CCACGAAATG GGCCACATCC AGTATTTCAT GCAGTACAAA
    GACTTACCTG TGGCCTTGAG GGAGGGTGCC AACCCCGGCT TCCATGAGGC CATTGGGGAC
    GTGCTAGCCC TCTCAGTGTC TACGCCCAAG CACCTGCACA GTCTCAACCT GCTGAGCAGT
    GAGGGTGGCA GCGACGAGCA TGACATCAAC TTTCTGATGA AGATGGCCCT TGACAAGATC
    GCCTTTATCC CCTTCAGCTA CCTCGTCGAT CAGTGGCGCT GGAGGGTATT TGATGGAAGC
    ATCACCAAGG AGAACTATAA CCAGGAGTGG TGGAGCCTCA GGCTGAAGTA CCAGGGCCTC
    TGCCCCCCAG TGCCCAGGAC TCAAGGTGAC TTTGACCCAG GGGCCAAGTT CCACATTCCT
    TCTAGCGTGC CTTACATCAG GTACTTTGTC AGCTTCATCA TCCAGTTCCA GTTCCACGAG
    GCACTGTGCC AGGCAGCTGG CCACACGGGC CCCCTGCACA AGTGTGACAT CTACCAGTCC
    AAGGAGGCCG GGCAGCGCCT GGCGACCGCC ATGAAGCTGG GCTTCAGTAG GCCGTGGCCG
    GAAGCCATGC AGCTGATCAC GGGCCAGCCC AACATGAGCG CCTCGGCCAT GTTGAGCTAC
    TTCAAGCCGC TGCTGGACTG GCTCCGCACG GAGAACGAGC TGCATGGGGA GAAGCTGGGC
    TGGCCGCAGT ACAACTGGAC GCCGAACTCC GCTCGCTCAG AAGGGCCCCT CCCAGACAGC
    GGCCGCGTCA GCTTCCTGGG CCTGGACCTG GATGCGCAGC AGGCCCGCGT GGGCCAGTGG
    CTGCTGCTCT TCCTGGGCAT CGCCCTGCTG GTAGCCACCC TGGGCCTCAG CCAGCGGCTC
    TTCAGCATCC GCCACCGCAG CCTCCACCGG CACTCCCACG GGCCCCAGTT CGGCTCCGAG
    GTGGAGCTGA GACACTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000780.1
    CDS35..3955
    Translation

    Target ORF information:

    RefSeq Version NM_000789.4
    Organism Homo sapiens(human)
    Definition Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000789.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGGGCCG CCTCGGGCCG CCGGGGGCCG GGGCTGCTGC TGCCGCTGCC GCTGCTGTTG 
    CTGCTGCCGC CGCAGCCCGC CCTGGCGTTG GACCCCGGGC TGCAGCCCGG CAACTTTTCT
    GCTGACGAGG CCGGGGCGCA GCTCTTCGCG CAGAGCTACA ACTCCAGCGC CGAACAGGTG
    CTGTTCCAGA GCGTGGCCGC CAGCTGGGCG CACGACACCA ACATCACCGC GGAGAATGCA
    AGGCGCCAGG AGGAAGCAGC CCTGCTCAGC CAGGAGTTTG CGGAGGCCTG GGGCCAGAAG
    GCCAAGGAGC TGTATGAACC GATCTGGCAG AACTTCACGG ACCCGCAGCT GCGCAGGATC
    ATCGGAGCTG TGCGCACCCT GGGCTCTGCC AACCTGCCCC TGGCTAAGCG GCAGCAGTAC
    AACGCCCTGC TAAGCAACAT GAGCAGGATC TACTCCACCG CCAAGGTCTG CCTCCCCAAC
    AAGACTGCCA CCTGCTGGTC CCTGGACCCA GATCTCACCA ACATCCTGGC TTCCTCGCGA
    AGCTACGCCA TGCTCCTGTT TGCCTGGGAG GGCTGGCACA ACGCTGCGGG CATCCCGCTG
    AAACCGCTGT ACGAGGATTT CACTGCCCTC AGCAATGAAG CCTACAAGCA GGACGGCTTC
    ACAGACACGG GGGCCTACTG GCGCTCCTGG TACAACTCCC CCACCTTCGA GGACGATCTG
    GAACACCTCT ACCAACAGCT AGAGCCCCTC TACCTGAACC TCCATGCCTT CGTCCGCCGC
    GCACTGCATC GCCGATACGG AGACAGATAC ATCAACCTCA GGGGACCCAT CCCTGCTCAT
    CTGCTGGGAG ACATGTGGGC CCAGAGCTGG GAAAACATCT ACGACATGGT GGTGCCTTTC
    CCAGACAAGC CCAACCTCGA TGTCACCAGT ACTATGCTGC AGCAGGGCTG GAACGCCACG
    CACATGTTCC GGGTGGCAGA GGAGTTCTTC ACCTCCCTGG AGCTCTCCCC CATGCCTCCC
    GAGTTCTGGG AAGGGTCGAT GCTGGAGAAG CCGGCCGACG GGCGGGAAGT GGTGTGCCAC
    GCCTCGGCTT GGGACTTCTA CAACAGGAAA GACTTCAGGA TCAAGCAGTG CACACGGGTC
    ACGATGGACC AGCTCTCCAC AGTGCACCAT GAGATGGGCC ATATACAGTA CTACCTGCAG
    TACAAGGATC TGCCCGTCTC CCTGCGTCGG GGGGCCAACC CCGGCTTCCA TGAGGCCATT
    GGGGACGTGC TGGCGCTCTC GGTCTCCACT CCTGAACATC TGCACAAAAT CGGCCTGCTG
    GACCGTGTCA CCAATGACAC GGAAAGTGAC ATCAATTACT TGCTAAAAAT GGCACTGGAA
    AAAATTGCCT TCCTGCCCTT TGGCTACTTG GTGGACCAGT GGCGCTGGGG GGTCTTTAGT
    GGGCGTACCC CCCCTTCCCG CTACAACTTC GACTGGTGGT ATCTTCGAAC CAAGTATCAG
    GGGATCTGTC CTCCTGTTAC CCGAAACGAA ACCCACTTTG ATGCTGGAGC TAAGTTTCAT
    GTTCCAAATG TGACACCATA CATCAGGTAC TTTGTGAGTT TTGTCCTGCA GTTCCAGTTC
    CATGAAGCCC TGTGCAAGGA GGCAGGCTAT GAGGGCCCAC TGCACCAGTG TGACATCTAC
    CGGTCCACCA AGGCAGGGGC CAAGCTCCGG AAGGTGCTGC AGGCTGGCTC CTCCAGGCCC
    TGGCAGGAGG TGCTGAAGGA CATGGTCGGC TTAGATGCCC TGGATGCCCA GCCGCTGCTC
    AAGTACTTCC AGCCAGTCAC CCAGTGGCTG CAGGAGCAGA ACCAGCAGAA CGGCGAGGTC
    CTGGGCTGGC CCGAGTACCA GTGGCACCCG CCGTTGCCTG ACAACTACCC GGAGGGCATA
    GACCTGGTGA CTGATGAGGC TGAGGCCAGC AAGTTTGTGG AGGAATATGA CCGGACATCC
    CAGGTGGTGT GGAACGAGTA TGCCGAGGCC AACTGGAACT ACAACACCAA CATCACCACA
    GAGACCAGCA AGATTCTGCT GCAGAAGAAC ATGCAAATAG CCAACCACAC CCTGAAGTAC
    GGCACCCAGG CCAGGAAGTT TGATGTGAAC CAGTTGCAGA ACACCACTAT CAAGCGGATC
    ATAAAGAAGG TTCAGGACCT AGAACGGGCA GCACTGCCTG CCCAGGAGCT GGAGGAGTAC
    AACAAGATCC TGTTGGATAT GGAAACCACC TACAGCGTGG CCACTGTGTG CCACCCGAAT
    GGCAGCTGCC TGCAGCTCGA GCCAGATCTG ACGAATGTGA TGGCCACGTC CCGGAAATAT
    GAAGACCTGT TATGGGCATG GGAGGGCTGG CGAGACAAGG CGGGGAGAGC CATCCTCCAG
    TTTTACCCGA AATACGTGGA ACTCATCAAC CAGGCTGCCC GGCTCAATGG CTATGTAGAT
    GCAGGGGACT CGTGGAGGTC TATGTACGAG ACACCATCCC TGGAGCAAGA CCTGGAGCGG
    CTCTTCCAGG AGCTGCAGCC ACTCTACCTC AACCTGCATG CCTACGTGCG CCGGGCCCTG
    CACCGTCACT ACGGGGCCCA GCACATCAAC CTGGAGGGGC CCATTCCTGC TCACCTGCTG
    GGGAACATGT GGGCGCAGAC CTGGTCCAAC ATCTATGACT TGGTGGTGCC CTTCCCTTCA
    GCCCCCTCGA TGGACACCAC AGAGGCTATG CTAAAGCAGG GCTGGACGCC CAGGAGGATG
    TTTAAGGAGG CTGATGATTT CTTCACCTCC CTGGGGCTGC TGCCCGTGCC TCCTGAGTTC
    TGGAACAAGT CGATGCTGGA GAAGCCAACC GACGGGCGGG AGGTGGTCTG CCACGCCTCG
    GCCTGGGACT TCTACAACGG CAAGGACTTC CGGATCAAGC AGTGCACCAC CGTGAACTTG
    GAGGACCTGG TGGTGGCCCA CCACGAAATG GGCCACATCC AGTATTTCAT GCAGTACAAA
    GACTTACCTG TGGCCTTGAG GGAGGGTGCC AACCCCGGCT TCCATGAGGC CATTGGGGAC
    GTGCTAGCCC TCTCAGTGTC TACGCCCAAG CACCTGCACA GTCTCAACCT GCTGAGCAGT
    GAGGGTGGCA GCGACGAGCA TGACATCAAC TTTCTGATGA AGATGGCCCT TGACAAGATC
    GCCTTTATCC CCTTCAGCTA CCTCGTCGAT CAGTGGCGCT GGAGGGTATT TGATGGAAGC
    ATCACCAAGG AGAACTATAA CCAGGAGTGG TGGAGCCTCA GGCTGAAGTA CCAGGGCCTC
    TGCCCCCCAG TGCCCAGGAC TCAAGGTGAC TTTGACCCAG GGGCCAAGTT CCACATTCCT
    TCTAGCGTGC CTTACATCAG GTACTTTGTC AGCTTCATCA TCCAGTTCCA GTTCCACGAG
    GCACTGTGCC AGGCAGCTGG CCACACGGGC CCCCTGCACA AGTGTGACAT CTACCAGTCC
    AAGGAGGCCG GGCAGCGCCT GGCGACCGCC ATGAAGCTGG GCTTCAGTAG GCCGTGGCCG
    GAAGCCATGC AGCTGATCAC GGGCCAGCCC AACATGAGCG CCTCGGCCAT GTTGAGCTAC
    TTCAAGCCGC TGCTGGACTG GCTCCGCACG GAGAACGAGC TGCATGGGGA GAAGCTGGGC
    TGGCCGCAGT ACAACTGGAC GCCGAACTCC GCTCGCTCAG AAGGGCCCCT CCCAGACAGC
    GGCCGCGTCA GCTTCCTGGG CCTGGACCTG GATGCGCAGC AGGCCCGCGT GGGCCAGTGG
    CTGCTGCTCT TCCTGGGCAT CGCCCTGCTG GTAGCCACCC TGGGCCTCAG CCAGCGGCTC
    TTCAGCATCC GCCACCGCAG CCTCCACCGG CACTCCCACG GGCCCCAGTT CGGCTCCGAG
    GTGGAGCTGA GACACTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.