CYB5A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CYB5A
Entrez Gene ID	1528
Full Name	cytochrome b5 type A
Synonyms	CYB5,MCB5
General protein information	Preferred Names cytochrome b5 type A Names cytochrome b5 cytochrome b5 type A (microsomal) type 1 cyt-b5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18q22.3
Summary	The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010].
Disorder MIM:	613218
Disorder Html:	?Methemoglobinemia, type IV, 250790 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_148923.3	NP_683725.1	cytochrome b5 isoform 1
NM_001914.3	NP_001905.1	cytochrome b5 isoform 2
NM_148923.4	NP_683725.1	cytochrome b5 isoform 1
NM_001190807.2	NP_001177736.1	cytochrome b5 isoform 3
XM_011525835.2	XP_011524137.1	cytochrome b5 isoform X1
XM_011525835.1	XP_011524137.1	cytochrome b5 isoform X1
NM_001190807.3	NP_001177736.1	cytochrome b5 isoform 3
NM_001914.4	NP_001905.1	cytochrome b5 isoform 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cyb5a	NP_071581.1
Danio rerio (zebrafish)	cyb5a	NP_998300.1
Mus musculus (house mouse)	Cyb5	NP_080073.1
Canis lupus familiaris (dog)	LOC102156618	XP_005630929.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007121	XP_308640.2
Homo sapiens (human)	CYB5A	NP_683725.1
Canis lupus familiaris (dog)	CYB5A	NP_001180227.1
Gallus gallus (chicken)	CYB5A	NP_001001748.1
Caenorhabditis elegans (roundworm)	cytb-5.1	NP_510335.2
Arabidopsis thaliana (thale cress)	CB5-E	NP_200168.1
Pan troglodytes (chimpanzee)	LOC735913	XP_001135885.1
	CYB5A	XP_001083378.1
Bos taurus (cattle)	CYB5A	NP_776458.1
Drosophila melanogaster (fruit fly)	Cyt-b5	NP_610294.1
Oryza sativa (rice)	Os10g0518200	NP_001065073.2
Xenopus tropicalis (tropical clawed frog)	cyb5a	NP_001015979.1

Gene Ontology
Bibliography

Related articles in PubMed

Kinetic processivity of the two-step oxidations of progesterone and pregnenolone to androgens by human cytochrome P450 17A1.
Gonzalez E, Guengerich FP
The Journal of biological chemistry292(32)13168-13185(2017 08)

Efficient Reduction of Vertebrate Cytoglobins by the Cytochrome b
Amdahl MB, Sparacino-Watkins CE, Corti P, Gladwin MT, Tejero J
Biochemistry56(30)3993-4004(2017 08)

A Homodimer Model Can Resolve the Conundrum as to How Cytochrome P450 Oxidoreductase and Cytochrome b5 Compete for the Same Binding Site on Cytochrome P450c17.
Holien JK, Parker MW, Conley AJ, Corbin CJ, Rodgers RJ, Martin LL
Current protein & peptide science18(5)515-521(2017)

Evidence that cytochrome b5 acts as a redox donor in CYP17A1 mediated androgen synthesis.
Duggal R, Liu Y, Gregory MC, Denisov IG, Kincaid JR, Sligar SG
Biochemical and biophysical research communications477(2)202-8(2016 08)

Membrane-Anchored Cytochrome P450 1A2-Cytochrome b5 Complex Features an X-Shaped Contact between Antiparallel Transmembrane Helices.
Je??bek P, Flori?n J, Mart?nek V
Chemical research in toxicology29(4)626-36(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest CYP17A1, with electron donor NADPH-P450 reductase, is inherently distributive enzyme but that some processivity is present; some 17alpha-hydroxy pregnenolone formed does not dissociate from CYP17A1 before conversion to dehydroepiandrosterone; CYB5A does not enhance reaction by decreasing k(off) of ligands of CYP17A1. (CYP17A1 = cytochrome P450, family 17, subfamily A, polypeptide 1; CYB5A = cytochrome b5)
Title: Kinetic processivity of the two-step oxidations of progesterone and pregnenolone to androgens by human cytochrome P450 17A1.

Data suggest that cytochrome b5 (CYB5) and cytochrome b5 reductase 3 (CYB5R3) can reduce human cytoglobin (CYGB) and zebrafish cytoglobins at rates up to 250-fold higher than those reported for the known physiological substrates, hemoglobin and myoglobin; the three proteins (CYB5+CYB5R3+CYGB) appear to constitute a metabolon involved in generation of nitric oxide.
Title: Efficient Reduction of Vertebrate Cytoglobins by the Cytochrome b

A homodimer model can resolve the conundrum as to how cytochrome P450 oxidoreductase and cytochrome b5 compete for the same binding site on cytochrome P450c17. (Review)
Title: A Homodimer Model Can Resolve the Conundrum as to How Cytochrome P450 Oxidoreductase and Cytochrome b5 Compete for the Same Binding Site on Cytochrome P450c17.

Thus, although Mn-b5 binds to CYP17A1, it is unable to enhance the lyase reaction, strongly suggesting that cyt b5 has a redox effector role in enhancement of the CYP17A1 mediated lyase reaction necessary for androgen synthesis.
Title: Evidence that cytochrome b5 acts as a redox donor in CYP17A1 mediated androgen synthesis.

This study constructed a model of the membrane-bound full-length human P450 1A2-cyt b5 complex. The model was assembled from several parts using a multiscale modeling approach covering all-atom and coarse-grained molecular dynamics (MD).
Title: Membrane-Anchored Cytochrome P450 1A2-Cytochrome b5 Complex Features an X-Shaped Contact between Antiparallel Transmembrane Helices.

The following CYB5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYB5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09495	NM_148923.4 Latest version!	Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu21228	NM_001914.4 Latest version!	Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu21228	XM_011525835.2 Latest version!	Homo sapiens cytochrome b5 type A (CYB5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu10453	NM_001190807.3 Latest version!	Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu09495	NM_148923.3	Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu21228	XM_011525835.1	Homo sapiens cytochrome b5 type A (microsomal) (CYB5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu10453	NM_001190807.2	Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu21228	NM_001914.3	Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CYB5A ( NM_148923.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_683725.1 Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09495
Clone ID Related Accession (Same CDS sequence)	NM_148923.3 , NM_148923.4
Accession Version	NM_148923.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-11
Organism	Homo sapiens(human)
Product	cytochrome b5 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112646.1, BC015182.1 and BG196223.1. On Jan 13, 2011 this sequence version replaced NM_148923.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.65968.1, SRR1163658.435893.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2152474, SAMEA2154125 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAGC AGTCGGACGA GGCCGTGAAG TACTACACCC TAGAGGAGAT TCAGAAGCAC 
AACCACAGCA AGAGCACCTG GCTGATCCTG CACCACAAGG TGTACGATTT GACCAAATTT 
CTGGAAGAGC ATCCTGGTGG GGAAGAAGTT TTAAGGGAAC AAGCTGGAGG TGACGCTACT 
GAGAACTTTG AGGATGTCGG GCACTCTACA GATGCCAGGG AAATGTCCAA AACATTCATC 
ATTGGGGAGC TCCATCCAGA TGACAGACCA AAGTTAAACA AGCCTCCGGA AACTCTTATC 
ACTACTATTG ATTCTAGTTC CAGTTGGTGG ACCAACTGGG TGATCCCTGC CATCTCTGCA 
GTGGCCGTCG CCTTGATGTA TCGCCTATAC ATGGCAGAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_683725.1
CDS	142..546
Translation	MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPETLITTIDSSSSWWTNWVIPAISAVAVALMYRLYMAED

Target ORF information:

RefSeq Version	NM_148923.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_148923.3

ORF Insert Sequence:

ATGGCAGAGC AGTCGGACGA GGCCGTGAAG TACTACACCC TAGAGGAGAT TCAGAAGCAC 
AACCACAGCA AGAGCACCTG GCTGATCCTG CACCACAAGG TGTACGATTT GACCAAATTT 
CTGGAAGAGC ATCCTGGTGG GGAAGAAGTT TTAAGGGAAC AAGCTGGAGG TGACGCTACT 
GAGAACTTTG AGGATGTCGG GCACTCTACA GATGCCAGGG AAATGTCCAA AACATTCATC 
ATTGGGGAGC TCCATCCAGA TGACAGACCA AAGTTAAACA AGCCTCCGGA AACTCTTATC 
ACTACTATTG ATTCTAGTTC CAGTTGGTGG ACCAACTGGG TGATCCCTGC CATCTCTGCA 
GTGGCCGTCG CCTTGATGTA TCGCCTATAC ATGGCAGAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYB5A ( NM_148923.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_683725.1 Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09495
Clone ID Related Accession (Same CDS sequence)	NM_148923.3 , NM_148923.4
Accession Version	NM_148923.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	cytochrome b5 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112646.1, BC015182.1, BG196223.1 and AC090398.12. On Nov 22, 2018 this sequence version replaced NM_148923.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BF700496.1, SRR1660807.220050.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2152474, SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000340533.9/ ENSP00000341625.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGAGC AGTCGGACGA GGCCGTGAAG TACTACACCC TAGAGGAGAT TCAGAAGCAC 
AACCACAGCA AGAGCACCTG GCTGATCCTG CACCACAAGG TGTACGATTT GACCAAATTT 
CTGGAAGAGC ATCCTGGTGG GGAAGAAGTT TTAAGGGAAC AAGCTGGAGG TGACGCTACT 
GAGAACTTTG AGGATGTCGG GCACTCTACA GATGCCAGGG AAATGTCCAA AACATTCATC 
ATTGGGGAGC TCCATCCAGA TGACAGACCA AAGTTAAACA AGCCTCCGGA AACTCTTATC 
ACTACTATTG ATTCTAGTTC CAGTTGGTGG ACCAACTGGG TGATCCCTGC CATCTCTGCA 
GTGGCCGTCG CCTTGATGTA TCGCCTATAC ATGGCAGAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_683725.1
CDS	89..493
Translation	MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPETLITTIDSSSSWWTNWVIPAISAVAVALMYRLYMAED

Target ORF information:

RefSeq Version	NM_148923.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_148923.4

ORF Insert Sequence:

ATGGCAGAGC AGTCGGACGA GGCCGTGAAG TACTACACCC TAGAGGAGAT TCAGAAGCAC 
AACCACAGCA AGAGCACCTG GCTGATCCTG CACCACAAGG TGTACGATTT GACCAAATTT 
CTGGAAGAGC ATCCTGGTGG GGAAGAAGTT TTAAGGGAAC AAGCTGGAGG TGACGCTACT 
GAGAACTTTG AGGATGTCGG GCACTCTACA GATGCCAGGG AAATGTCCAA AACATTCATC 
ATTGGGGAGC TCCATCCAGA TGACAGACCA AAGTTAAACA AGCCTCCGGA AACTCTTATC 
ACTACTATTG ATTCTAGTTC CAGTTGGTGG ACCAACTGGG TGATCCCTGC CATCTCTGCA 
GTGGCCGTCG CCTTGATGTA TCGCCTATAC ATGGCAGAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYB5A ( NM_001914.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001905.1 Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21228
Clone ID Related Accession (Same CDS sequence)	NM_001914.3 , XM_011525835.2 , XM_011525835.1 , NM_001914.4
Accession Version	NM_001914.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	cytochrome b5 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112646.1, M60174.1, AC090398.12 and BG196223.1. This sequence is a reference standard in the RefSeqGene project. On or before Aug 31, 2013 this sequence version replaced XR_245456.1, NM_001914.2. Transcript Variant: This variant (2) has an additional exon in the 3' coding region, which introduces a stop codon, as compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.93391.1, SRR1803613.222473.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159080 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAGC AGTCGGACGA GGCCGTGAAG TACTACACCC TAGAGGAGAT TCAGAAGCAC 
AACCACAGCA AGAGCACCTG GCTGATCCTG CACCACAAGG TGTACGATTT GACCAAATTT 
CTGGAAGAGC ATCCTGGTGG GGAAGAAGTT TTAAGGGAAC AAGCTGGAGG TGACGCTACT 
GAGAACTTTG AGGATGTCGG GCACTCTACA GATGCCAGGG AAATGTCCAA AACATTCATC 
ATTGGGGAGC TCCATCCAGA TGACAGACCA AAGTTAAACA AGCCTCCGGA ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001905.1
CDS	142..438
Translation	MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPEP

Target ORF information:

RefSeq Version	NM_001914.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001914.3

ORF Insert Sequence:

ATGGCAGAGC AGTCGGACGA GGCCGTGAAG TACTACACCC TAGAGGAGAT TCAGAAGCAC 
AACCACAGCA AGAGCACCTG GCTGATCCTG CACCACAAGG TGTACGATTT GACCAAATTT 
CTGGAAGAGC ATCCTGGTGG GGAAGAAGTT TTAAGGGAAC AAGCTGGAGG TGACGCTACT 
GAGAACTTTG AGGATGTCGG GCACTCTACA GATGCCAGGG AAATGTCCAA AACATTCATC 
ATTGGGGAGC TCCATCCAGA TGACAGACCA AAGTTAAACA AGCCTCCGGA ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYB5A ( NM_001914.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001905.1 Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21228
Clone ID Related Accession (Same CDS sequence)	NM_001914.3 , XM_011525835.2 , XM_011525835.1 , NM_001914.4
Accession Version	NM_001914.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	cytochrome b5 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112646.1, M60174.1, AC090398.12 and BG196223.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001914.3. Transcript Variant: This variant (2) has an additional exon in the 3' coding region, which introduces a stop codon, as compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.222473.1, M60174.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159080 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAGC AGTCGGACGA GGCCGTGAAG TACTACACCC TAGAGGAGAT TCAGAAGCAC 
AACCACAGCA AGAGCACCTG GCTGATCCTG CACCACAAGG TGTACGATTT GACCAAATTT 
CTGGAAGAGC ATCCTGGTGG GGAAGAAGTT TTAAGGGAAC AAGCTGGAGG TGACGCTACT 
GAGAACTTTG AGGATGTCGG GCACTCTACA GATGCCAGGG AAATGTCCAA AACATTCATC 
ATTGGGGAGC TCCATCCAGA TGACAGACCA AAGTTAAACA AGCCTCCGGA ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001905.1
CDS	89..385
Translation	MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPEP

Target ORF information:

RefSeq Version	NM_001914.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001914.4

ORF Insert Sequence:

ATGGCAGAGC AGTCGGACGA GGCCGTGAAG TACTACACCC TAGAGGAGAT TCAGAAGCAC 
AACCACAGCA AGAGCACCTG GCTGATCCTG CACCACAAGG TGTACGATTT GACCAAATTT 
CTGGAAGAGC ATCCTGGTGG GGAAGAAGTT TTAAGGGAAC AAGCTGGAGG TGACGCTACT 
GAGAACTTTG AGGATGTCGG GCACTCTACA GATGCCAGGG AAATGTCCAA AACATTCATC 
ATTGGGGAGC TCCATCCAGA TGACAGACCA AAGTTAAACA AGCCTCCGGA ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYB5A ( XM_011525835.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524137.1 Homo sapiens cytochrome b5 type A (CYB5A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu21228
Clone ID Related Accession (Same CDS sequence)	NM_001914.3 , XM_011525835.2 , XM_011525835.1 , NM_001914.4
Accession Version	XM_011525835.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cytochrome b5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AGTCGGACGA GGCCGTGAAG TACTACACCC TAGAGGAGAT TCAGAAGCAC 
AACCACAGCA AGAGCACCTG GCTGATCCTG CACCACAAGG TGTACGATTT GACCAAATTT 
CTGGAAGAGC ATCCTGGTGG GGAAGAAGTT TTAAGGGAAC AAGCTGGAGG TGACGCTACT 
GAGAACTTTG AGGATGTCGG GCACTCTACA GATGCCAGGG AAATGTCCAA AACATTCATC 
ATTGGGGAGC TCCATCCAGA TGACAGACCA AAGTTAAACA AGCCTCCGGA ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524137.1
CDS	106..402
Translation	MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPEP

Target ORF information:

RefSeq Version	XM_011525835.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome b5 type A (CYB5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525835.2

ORF Insert Sequence:

ATGGCAGAGC AGTCGGACGA GGCCGTGAAG TACTACACCC TAGAGGAGAT TCAGAAGCAC 
AACCACAGCA AGAGCACCTG GCTGATCCTG CACCACAAGG TGTACGATTT GACCAAATTT 
CTGGAAGAGC ATCCTGGTGG GGAAGAAGTT TTAAGGGAAC AAGCTGGAGG TGACGCTACT 
GAGAACTTTG AGGATGTCGG GCACTCTACA GATGCCAGGG AAATGTCCAA AACATTCATC 
ATTGGGGAGC TCCATCCAGA TGACAGACCA AAGTTAAACA AGCCTCCGGA ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYB5A ( XM_011525835.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524137.1 Homo sapiens cytochrome b5 type A (microsomal) (CYB5A), transcript variant X1, mRNA.

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CloneID	OHu21228
Clone ID Related Accession (Same CDS sequence)	NM_001914.3 , XM_011525835.2 , XM_011525835.1 , NM_001914.4
Accession Version	XM_011525835.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	cytochrome b5 isoform X1
Comment

ATGGCAGAGC AGTCGGACGA GGCCGTGAAG TACTACACCC TAGAGGAGAT TCAGAAGCAC 
AACCACAGCA AGAGCACCTG GCTGATCCTG CACCACAAGG TGTACGATTT GACCAAATTT 
CTGGAAGAGC ATCCTGGTGG GGAAGAAGTT TTAAGGGAAC AAGCTGGAGG TGACGCTACT 
GAGAACTTTG AGGATGTCGG GCACTCTACA GATGCCAGGG AAATGTCCAA AACATTCATC 
ATTGGGGAGC TCCATCCAGA TGACAGACCA AAGTTAAACA AGCCTCCGGA ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524137.1
Translation	MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPEP

Target ORF information:

RefSeq Version	XM_011525835.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens cytochrome b5 type A (microsomal) (CYB5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525835.1

ORF Insert Sequence:

ATGGCAGAGC AGTCGGACGA GGCCGTGAAG TACTACACCC TAGAGGAGAT TCAGAAGCAC 
AACCACAGCA AGAGCACCTG GCTGATCCTG CACCACAAGG TGTACGATTT GACCAAATTT 
CTGGAAGAGC ATCCTGGTGG GGAAGAAGTT TTAAGGGAAC AAGCTGGAGG TGACGCTACT 
GAGAACTTTG AGGATGTCGG GCACTCTACA GATGCCAGGG AAATGTCCAA AACATTCATC 
ATTGGGGAGC TCCATCCAGA TGACAGACCA AAGTTAAACA AGCCTCCGGA ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYB5A ( NM_001190807.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177736.1 Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu10453
Clone ID Related Accession (Same CDS sequence)	NM_001190807.2 , NM_001190807.3
Accession Version	NM_001190807.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	cytochrome b5 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112646.1, CA771478.1, AC090398.12 and BG196223.1. On Jan 13, 2011 this sequence version replaced NM_001190807.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CA771478.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159080, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAGC AGTCGGACGA GGCCGTGAAG TACTACACCC TAGAGGAGAT TCAGAAGCAC 
AACCACAGCA AGAGCACCTG GCTGATCCTG CACCACAAGG TGTACGATTT GACCAAATTT 
CTGGAAGAGC ATCCTGGTGG GGAAGAAGTT TTAAGGGAAC AAGCTGGAGG TGACGCTACT 
GAGAACTTTG AGGATGTCGG GCACTCTACA GATGCCAGGG AAATGTCCAA AACATTCATC 
ATTGGGGAGC TCCATCCAGA AACTCTTATC ACTACTATTG ATTCTAGTTC CAGTTGGTGG 
ACCAACTGGG TGATCCCTGC CATCTCTGCA GTGGCCGTCG CCTTGATGTA TCGCCTATAC 
ATGGCAGAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177736.1
CDS	142..516
Translation	MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPETLITTIDSSSSWWTNWVIPAISAVAVALMYRLYMAED

Target ORF information:

RefSeq Version	NM_001190807.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190807.2

ORF Insert Sequence:

ATGGCAGAGC AGTCGGACGA GGCCGTGAAG TACTACACCC TAGAGGAGAT TCAGAAGCAC 
AACCACAGCA AGAGCACCTG GCTGATCCTG CACCACAAGG TGTACGATTT GACCAAATTT 
CTGGAAGAGC ATCCTGGTGG GGAAGAAGTT TTAAGGGAAC AAGCTGGAGG TGACGCTACT 
GAGAACTTTG AGGATGTCGG GCACTCTACA GATGCCAGGG AAATGTCCAA AACATTCATC 
ATTGGGGAGC TCCATCCAGA AACTCTTATC ACTACTATTG ATTCTAGTTC CAGTTGGTGG 
ACCAACTGGG TGATCCCTGC CATCTCTGCA GTGGCCGTCG CCTTGATGTA TCGCCTATAC 
ATGGCAGAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYB5A ( NM_001190807.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177736.1 Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu10453
Clone ID Related Accession (Same CDS sequence)	NM_001190807.2 , NM_001190807.3
Accession Version	NM_001190807.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	cytochrome b5 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112646.1, CA771478.1, AC090398.12 and BG196223.1. On May 31, 2019 this sequence version replaced NM_001190807.2. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CA771478.1, DRR138522.388966.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159080, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAGC AGTCGGACGA GGCCGTGAAG TACTACACCC TAGAGGAGAT TCAGAAGCAC 
AACCACAGCA AGAGCACCTG GCTGATCCTG CACCACAAGG TGTACGATTT GACCAAATTT 
CTGGAAGAGC ATCCTGGTGG GGAAGAAGTT TTAAGGGAAC AAGCTGGAGG TGACGCTACT 
GAGAACTTTG AGGATGTCGG GCACTCTACA GATGCCAGGG AAATGTCCAA AACATTCATC 
ATTGGGGAGC TCCATCCAGA AACTCTTATC ACTACTATTG ATTCTAGTTC CAGTTGGTGG 
ACCAACTGGG TGATCCCTGC CATCTCTGCA GTGGCCGTCG CCTTGATGTA TCGCCTATAC 
ATGGCAGAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177736.1
CDS	89..463
Translation	MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPETLITTIDSSSSWWTNWVIPAISAVAVALMYRLYMAED

Target ORF information:

RefSeq Version	NM_001190807.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome b5 type A (CYB5A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190807.3

ORF Insert Sequence:

ATGGCAGAGC AGTCGGACGA GGCCGTGAAG TACTACACCC TAGAGGAGAT TCAGAAGCAC 
AACCACAGCA AGAGCACCTG GCTGATCCTG CACCACAAGG TGTACGATTT GACCAAATTT 
CTGGAAGAGC ATCCTGGTGG GGAAGAAGTT TTAAGGGAAC AAGCTGGAGG TGACGCTACT 
GAGAACTTTG AGGATGTCGG GCACTCTACA GATGCCAGGG AAATGTCCAA AACATTCATC 
ATTGGGGAGC TCCATCCAGA AACTCTTATC ACTACTATTG ATTCTAGTTC CAGTTGGTGG 
ACCAACTGGG TGATCCCTGC CATCTCTGCA GTGGCCGTCG CCTTGATGTA TCGCCTATAC 
ATGGCAGAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Kinetic processivity of the two-step oxidations of progesterone and pregnenolone to androgens by human cytochrome P450 17A1.
The Journal of biological chemistry292(32)13168-13185(2017 08)
Gonzalez E,Guengerich FP

Efficient Reduction of Vertebrate Cytoglobins by the Cytochrome b
Biochemistry56(30)3993-4004(2017 08)
Amdahl MB,Sparacino-Watkins CE,Corti P,Gladwin MT,Tejero J

A Homodimer Model Can Resolve the Conundrum as to How Cytochrome P450 Oxidoreductase and Cytochrome b5 Compete for the Same Binding Site on Cytochrome P450c17.
Current protein & peptide science18(5)515-521(2017)
Holien JK,Parker MW,Conley AJ,Corbin CJ,Rodgers RJ,Martin LL

Evidence that cytochrome b5 acts as a redox donor in CYP17A1 mediated androgen synthesis.
Biochemical and biophysical research communications477(2)202-8(2016 08)
Duggal R,Liu Y,Gregory MC,Denisov IG,Kincaid JR,Sligar SG

Membrane-Anchored Cytochrome P450 1A2-Cytochrome b5 Complex Features an X-Shaped Contact between Antiparallel Transmembrane Helices.
Chemical research in toxicology29(4)626-36(2016 Apr)
Je??bek P,Flori?n J,Mart?nek V

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CYB5A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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