CRYGD cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRYGD
Entrez Gene ID	1421
Full Name	crystallin gamma D
Synonyms	CACA,CCA3,CCP,CRYG4,CTRCT4,PCC,cry-g-D
General protein information	Preferred Names crystallin gamma D Names gamma-crystallin D gamma crystallin 4 gamma-D-crystallin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q33.3
Summary	Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. [provided by RefSeq, Jul 2008].
Disorder MIM:	123690
Disorder Html:	Cataract 4, multiple types, 115700 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_006891.3	NP_008822.2	gamma-crystallin D
NM_006891.4	NP_008822.2	gamma-crystallin D

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Crygd	NP_031802.2
	CRYGD	XP_001100337.2
Canis lupus familiaris (dog)	CRYGD	NP_001106640.1
Rattus norvegicus (Norway rat)	Crygd	NP_149086.1
Danio rerio (zebrafish)	crygmxl1	NP_001019594.1
Homo sapiens (human)	CRYGD	NP_008822.2
Pan troglodytes (chimpanzee)	CRYGD	NP_001129137.1

Gene Ontology
Bibliography

Related articles in PubMed

Segregation of a?novel p.(Ser270Tyr) MAF mutation and p.(Tyr56?) CRYGD variant in a family with dominantly inherited congenital cataracts.
Dudakova L, Stranecky V, Ulmanova O, Hlavova E, Trkov? M, Vincent AL, Liskova P
Molecular biology reports44(6)435-440(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we identified two heterozygous rare variants in genes that are involved in early cataract development; the novel c.809C>A; p.(Ser270Tyr) in MAF and the c.168C>G; p.(Tyr56 *) variant in CRYGD, previously reported as pathogenic
Title: Segregation of a?novel p.(Ser270Tyr) MAF mutation and p.(Tyr56?) CRYGD variant in a family with dominantly inherited congenital cataracts.

the mechanism of aggregation of two gammaD-crystallin mutants, W42R and W42Q: the former a congenital cataract mutation
Title: An Internal Disulfide Locks a Misfolded Aggregation-prone Intermediate in Cataract-linked Mutants of Human ?D-Crystallin.

The nonsense mutation c.471G>A of the CRYGD gene probably underlies the congenital cataract in the pedigree
Title: [A novel pathogenic mutation of CRYGD gene in a congenital cataract family].

Aggregation of Trp > Glu point mutants of human gamma-D crystallin provides a model for hereditary or UV-induced cataract.
Title: Aggregation of Trp?>?Glu point mutants of human gamma-D crystallin provides a model for hereditary or UV-induced cataract.

Mutational analysis of CRYGD identified a recurrent (p.P24T) mutation in two unrelated families with congenital coralliform cataracts and three novel (p.Q101X, p.E104fsX4 and p.E135X) mutations in three families with congenital nuclear cataracts.
Title: Novel mutations in CRYGD are associated with congenital cataracts in Chinese families.

The following CRYGD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRYGD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19598	NM_006891.4 Latest version!	Homo sapiens crystallin gamma D (CRYGD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu19598	NM_006891.3	Homo sapiens crystallin gamma D (CRYGD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRYGD ( NM_006891.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_008822.2 Homo sapiens crystallin gamma D (CRYGD), mRNA.

Price & Availability↑

CloneID	OHu19598
Clone ID Related Accession (Same CDS sequence)	NM_006891.3 , NM_006891.4
Accession Version	NM_006891.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	gamma-crystallin D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC117338.1, U66583.1, EL946552.1 and CD674533.1. This sequence is a reference standard in the RefSeqGene project. On Apr 2, 2008 this sequence version replaced NM_006891.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC117338.1, CD676210.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAGA TCACCCTCTA CGAGGACCGG GGCTTCCAGG GCCGCCACTA TGAATGCAGC 
AGCGACCACC CCAACCTGCA GCCCTACTTG AGCCGCTGCA ACTCGGCGCG CGTGGACAGC 
GGCTGCTGGA TGCTCTATGA GCAGCCCAAC TACTCGGGCC TCCAGTACTT CCTGCGCCGC 
GGCGACTATG CCGACCACCA GCAGTGGATG GGCCTCAGCG ACTCGGTCCG CTCCTGCCGC 
CTCATCCCCC ACTCTGGCTC TCACAGGATC AGACTCTATG AGAGAGAGGA CTACAGAGGC 
CAGATGATAG AGTTCACTGA GGACTGCTCC TGTCTTCAGG ACCGCTTCCG CTTCAATGAA 
ATCCACTCCC TCAACGTGCT GGAGGGCTCC TGGGTCCTCT ACGAGCTGTC CAACTACCGA 
GGACGGCAGT ACCTGCTGAT GCCAGGGGAC TATAGGCGCT ACCAGGACTG GGGGGCCACG 
AATGCCAGAG TGGGCTCTCT GAGGAGAGTC ATAGATTTCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008822.2
CDS	117..641
Translation	MGKITLYEDRGFQGRHYECSSDHPNLQPYLSRCNSARVDSGCWMLYEQPNYSGLQYFLRRGDYADHQQWMGLSDSVRSCRLIPHSGSHRIRLYEREDYRGQMIEFTEDCSCLQDRFRFNEIHSLNVLEGSWVLYELSNYRGRQYLLMPGDYRRYQDWGATNARVGSLRRVIDFS

Target ORF information:

RefSeq Version	NM_006891.3
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin gamma D (CRYGD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006891.3

ORF Insert Sequence:

ATGGGGAAGA TCACCCTCTA CGAGGACCGG GGCTTCCAGG GCCGCCACTA TGAATGCAGC 
AGCGACCACC CCAACCTGCA GCCCTACTTG AGCCGCTGCA ACTCGGCGCG CGTGGACAGC 
GGCTGCTGGA TGCTCTATGA GCAGCCCAAC TACTCGGGCC TCCAGTACTT CCTGCGCCGC 
GGCGACTATG CCGACCACCA GCAGTGGATG GGCCTCAGCG ACTCGGTCCG CTCCTGCCGC 
CTCATCCCCC ACTCTGGCTC TCACAGGATC AGACTCTATG AGAGAGAGGA CTACAGAGGC 
CAGATGATAG AGTTCACTGA GGACTGCTCC TGTCTTCAGG ACCGCTTCCG CTTCAATGAA 
ATCCACTCCC TCAACGTGCT GGAGGGCTCC TGGGTCCTCT ACGAGCTGTC CAACTACCGA 
GGACGGCAGT ACCTGCTGAT GCCAGGGGAC TATAGGCGCT ACCAGGACTG GGGGGCCACG 
AATGCCAGAG TGGGCTCTCT GAGGAGAGTC ATAGATTTCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYGD ( NM_006891.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_008822.2 Homo sapiens crystallin gamma D (CRYGD), mRNA.

Price & Availability↑

CloneID	OHu19598
Clone ID Related Accession (Same CDS sequence)	NM_006891.3 , NM_006891.4
Accession Version	NM_006891.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	gamma-crystallin D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC117338.1, U66583.1, EL946552.1 and CD674533.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_006891.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC117338.1, CD676210.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264376.5/ ENSP00000264376.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGAAGA TCACCCTCTA CGAGGACCGG GGCTTCCAGG GCCGCCACTA TGAATGCAGC 
AGCGACCACC CCAACCTGCA GCCCTACTTG AGCCGCTGCA ACTCGGCGCG CGTGGACAGC 
GGCTGCTGGA TGCTCTATGA GCAGCCCAAC TACTCGGGCC TCCAGTACTT CCTGCGCCGC 
GGCGACTATG CCGACCACCA GCAGTGGATG GGCCTCAGCG ACTCGGTCCG CTCCTGCCGC 
CTCATCCCCC ACTCTGGCTC TCACAGGATC AGACTCTATG AGAGAGAGGA CTACAGAGGC 
CAGATGATAG AGTTCACTGA GGACTGCTCC TGTCTTCAGG ACCGCTTCCG CTTCAATGAA 
ATCCACTCCC TCAACGTGCT GGAGGGCTCC TGGGTCCTCT ACGAGCTGTC CAACTACCGA 
GGACGGCAGT ACCTGCTGAT GCCAGGGGAC TATAGGCGCT ACCAGGACTG GGGGGCCACG 
AATGCCAGAG TGGGCTCTCT GAGGAGAGTC ATAGATTTCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008822.2
CDS	52..576
Translation	MGKITLYEDRGFQGRHYECSSDHPNLQPYLSRCNSARVDSGCWMLYEQPNYSGLQYFLRRGDYADHQQWMGLSDSVRSCRLIPHSGSHRIRLYEREDYRGQMIEFTEDCSCLQDRFRFNEIHSLNVLEGSWVLYELSNYRGRQYLLMPGDYRRYQDWGATNARVGSLRRVIDFS

Target ORF information:

RefSeq Version	NM_006891.4
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin gamma D (CRYGD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006891.4

ORF Insert Sequence:

ATGGGGAAGA TCACCCTCTA CGAGGACCGG GGCTTCCAGG GCCGCCACTA TGAATGCAGC 
AGCGACCACC CCAACCTGCA GCCCTACTTG AGCCGCTGCA ACTCGGCGCG CGTGGACAGC 
GGCTGCTGGA TGCTCTATGA GCAGCCCAAC TACTCGGGCC TCCAGTACTT CCTGCGCCGC 
GGCGACTATG CCGACCACCA GCAGTGGATG GGCCTCAGCG ACTCGGTCCG CTCCTGCCGC 
CTCATCCCCC ACTCTGGCTC TCACAGGATC AGACTCTATG AGAGAGAGGA CTACAGAGGC 
CAGATGATAG AGTTCACTGA GGACTGCTCC TGTCTTCAGG ACCGCTTCCG CTTCAATGAA 
ATCCACTCCC TCAACGTGCT GGAGGGCTCC TGGGTCCTCT ACGAGCTGTC CAACTACCGA 
GGACGGCAGT ACCTGCTGAT GCCAGGGGAC TATAGGCGCT ACCAGGACTG GGGGGCCACG 
AATGCCAGAG TGGGCTCTCT GAGGAGAGTC ATAGATTTCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Segregation of a?novel p.(Ser270Tyr) MAF mutation and p.(Tyr56?) CRYGD variant in a family with dominantly inherited congenital cataracts.
Molecular biology reports44(6)435-440(2017 Dec)
Dudakova L,Stranecky V,Ulmanova O,Hlavova E,Trkov? M,Vincent AL,Liskova P

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CRYGD cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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