AFAP1L1 cDNA ORF clone, Homo sapiens(human)

The following AFAP1L1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFAP1L1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu12430 NM_152406.4
Latest version!
Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu75735 XM_011537558.2
Latest version!
Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu100402 NM_001323063.1
Latest version!
Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu95655 XM_017009036.1
Latest version!
Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $391.30
$559.00
OHu11195 NM_001146337.3
Latest version!
Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu39817 NM_001323062.2
Latest version!
Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
View Old Accession Versions >>
OHu12430 NM_152406.2 Homo sapiens actin filament associated protein 1-like 1 (AFAP1L1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu12430 NM_152406.3 Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu75735 XM_011537558.1 Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu11195 NM_001146337.2 Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu39817 NM_001323062.1 Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu12430
    Clone ID Related Accession (Same CDS sequence) NM_152406.2 , NM_152406.3 , NM_152406.4
    Accession Version NM_152406.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product actin filament-associated protein 1-like 1 isoform 1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACCGAG GCCAGGTGCT GGAGCAGCTG CTCCCAGAGC TCACCGGGCT GCTCAGCCTC 
    CTGGACCACG AGTACCTCAG CGATACCACC CTGGAAAAGA AGATGGCCGT GGCCTCCATC
    CTGCAGAGCC TGCAGCCCCT TCCAGCAAAG GAGGTCTCCT ACCTGTATGT GAACACAGCA
    GACCTCCACT CGGGGCCCAG CTTCGTGGAA TCCCTCTTTG AAGAATTTGA CTGTGACCTG
    AGTGACCTTC GGGACATGCC AGAGGATGAT GGGGAGCCCA GCAAAGGAGC CAGCCCTGAG
    CTAGCCAAGA GCCCACGCCT GAGAAACGCG GCCGACCTGC CTCCACCGCT CCCCAACAAG
    CCTCCCCCTG AGGACTACTA TGAAGAGGCC CTTCCTCTGG GACCCGGCAA GTCGCCTGAG
    TACATCAGCT CCCACAATGG CTGCAGCCCC TCACACTCGA TTGTGGATGG CTACTATGAG
    GACGCAGACA GCAGCTACCC TGCAACCAGG GTGAACGGCG AGCTTAAGAG CTCCTATAAT
    GACTCTGACG CAATGAGCAG CTCCTATGAG TCCTACGATG AAGAGGAGGA GGAAGGGAAG
    AGCCCGCAGC CCCGACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAA
    TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGTTTCG GGCAGTGGGC CAAGCAGCTG
    ACGGTCATCA GGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGATCG GCAGCCACAT
    CTGAGGTTGG CACTGGATAC CTGCAGCATC ATCTACGTGC CCAAGGACAG CCGGCACAAG
    AGGCACGAGC TGCGTTTCAC CCAGGGGGCT ACCGAGGTCT TGGTGCTGGC ACTGCAGAGC
    CGAGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAAG TGAGCAAGCC AGTTGGGGGA
    GCTGAGGGAG TGGAGGTCCC CAGATCCCCA GTCCTCCTGT GCAAGTTGGA CCTGGACAAG
    AGGCTGTCCC AAGAGAAGCA GACCTCAGAT TCTGACAGCG TGGGTGTGGG TGACAACTGT
    TCTACCCTTG GCCGCCGGGA GACCTGTGAT CACGGCAAAG GGAAGAAGAG CAGCCTGGCA
    GAACTGAAGG GCTCAATGAG CAGGGCTGCG GGCCGCAAGA TCACCCGTAT CATTGGCTTC
    TCCAAGAAGA AGACACTGGC CGATGACCTG CAGACGTCCT CCACCGAGGA GGAGGTTCCC
    TGCTGTGGCT ACCTGAACGT GCTGGTGAAC CAGGGCTGGA AGGAACGCTG GTGCCGCCTG
    AAGTGCAACA CTCTGTATTT CCACAAGGAT CACATGGACC TGCGAACCCA TGTGAACGCC
    ATCGCCCTGC AAGGCTGTGA GGTGGCCCCG GGCTTTGGGC CCCGACACCC ATTTGCCTTC
    AGGATCCTGC GCAACCGGCA GGAGGTGGCC ATCTTGGAGG CAAGCTGTTC AGAGGACATG
    GGTCGCTGGC TCGGGCTGCT GCTGGTGGAG ATGGGCTCCA GAGTCACTCC GGAGGCGCTG
    CACTATGACT ACGTGGATGT GGAGACCTTA ACCAGCATCG TCAGTGCTGG GCGCAACTCC
    TTCCTATATG CAAGATCCTG CCAGAATCAG TGGCCTGAGC CCCGAGTCTA TGATGATGTT
    CCTTATGAAA AGATGCAGGA CGAGGAGCCC GAGCGCCCCA CAGGGGCCCA GGTGAAGCGT
    CACGCCTCCT CCTGCAGTGA GAAGTCCCAT CGTGTGGACC CGCAGGTCAA AGTCAAACGC
    CACGCCTCCA GTGCCAATCA ATACAAGTAT GGCAAGAACC GAGCCGAGGA GGATGCCCGG
    AGGTACTTGG TAGAAAAAGA GAAGCTGGAG AAAGAGAAAG AGACGATTCG GACAGAGCTG
    ATAGCACTGA GACAGGAGAA GAGGGAACTG AAGGAAGCCA TTCGGAGCAG CCCAGGAGCA
    AAATTAAAGG CTCTGGAAGA AGCCGTGGCC ACCCTGGAAG CTCAGTGTCG GGCAAAGGAG
    GAGCGCCGGA TTGACCTGGA GCTGAAGCTG GTGGCTGTGA AGGAGCGCTT GCAGCAGTCC
    CTGGCAGGAG GGCCAGCCCT GGGGCTCTCC GTGAGCAGCA AGCCCAAGAG TGGGGAAACT
    GCAAATAAAC CCCAGAACAG CGTTCCAGAG CAACCTCTCC CTGTCAACTG TGTTTCTGAG
    CTGAGGAAGA GGAGCCCATC CATCGTAGCC TCCAACCAAG GAAGGGTGCT ACAGAAAGCC
    AAGGAATGGG AAATGAAGAA GACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_689619.1
    Translation

    Target ORF information:

    RefSeq Version NM_152406.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens actin filament associated protein 1-like 1 (AFAP1L1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_152406.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACCGAG GCCAGGTGCT GGAGCAGCTG CTCCCAGAGC TCACCGGGCT GCTCAGCCTC 
    CTGGACCACG AGTACCTCAG CGATACCACC CTGGAAAAGA AGATGGCCGT GGCCTCCATC
    CTGCAGAGCC TGCAGCCCCT TCCAGCAAAG GAGGTCTCCT ACCTGTATGT GAACACAGCA
    GACCTCCACT CGGGGCCCAG CTTCGTGGAA TCCCTCTTTG AAGAATTTGA CTGTGACCTG
    AGTGACCTTC GGGACATGCC AGAGGATGAT GGGGAGCCCA GCAAAGGAGC CAGCCCTGAG
    CTAGCCAAGA GCCCACGCCT GAGAAACGCG GCCGACCTGC CTCCACCGCT CCCCAACAAG
    CCTCCCCCTG AGGACTACTA TGAAGAGGCC CTTCCTCTGG GACCCGGCAA GTCGCCTGAG
    TACATCAGCT CCCACAATGG CTGCAGCCCC TCACACTCGA TTGTGGATGG CTACTATGAG
    GACGCAGACA GCAGCTACCC TGCAACCAGG GTGAACGGCG AGCTTAAGAG CTCCTATAAT
    GACTCTGACG CAATGAGCAG CTCCTATGAG TCCTACGATG AAGAGGAGGA GGAAGGGAAG
    AGCCCGCAGC CCCGACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAA
    TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGTTTCG GGCAGTGGGC CAAGCAGCTG
    ACGGTCATCA GGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGATCG GCAGCCACAT
    CTGAGGTTGG CACTGGATAC CTGCAGCATC ATCTACGTGC CCAAGGACAG CCGGCACAAG
    AGGCACGAGC TGCGTTTCAC CCAGGGGGCT ACCGAGGTCT TGGTGCTGGC ACTGCAGAGC
    CGAGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAAG TGAGCAAGCC AGTTGGGGGA
    GCTGAGGGAG TGGAGGTCCC CAGATCCCCA GTCCTCCTGT GCAAGTTGGA CCTGGACAAG
    AGGCTGTCCC AAGAGAAGCA GACCTCAGAT TCTGACAGCG TGGGTGTGGG TGACAACTGT
    TCTACCCTTG GCCGCCGGGA GACCTGTGAT CACGGCAAAG GGAAGAAGAG CAGCCTGGCA
    GAACTGAAGG GCTCAATGAG CAGGGCTGCG GGCCGCAAGA TCACCCGTAT CATTGGCTTC
    TCCAAGAAGA AGACACTGGC CGATGACCTG CAGACGTCCT CCACCGAGGA GGAGGTTCCC
    TGCTGTGGCT ACCTGAACGT GCTGGTGAAC CAGGGCTGGA AGGAACGCTG GTGCCGCCTG
    AAGTGCAACA CTCTGTATTT CCACAAGGAT CACATGGACC TGCGAACCCA TGTGAACGCC
    ATCGCCCTGC AAGGCTGTGA GGTGGCCCCG GGCTTTGGGC CCCGACACCC ATTTGCCTTC
    AGGATCCTGC GCAACCGGCA GGAGGTGGCC ATCTTGGAGG CAAGCTGTTC AGAGGACATG
    GGTCGCTGGC TCGGGCTGCT GCTGGTGGAG ATGGGCTCCA GAGTCACTCC GGAGGCGCTG
    CACTATGACT ACGTGGATGT GGAGACCTTA ACCAGCATCG TCAGTGCTGG GCGCAACTCC
    TTCCTATATG CAAGATCCTG CCAGAATCAG TGGCCTGAGC CCCGAGTCTA TGATGATGTT
    CCTTATGAAA AGATGCAGGA CGAGGAGCCC GAGCGCCCCA CAGGGGCCCA GGTGAAGCGT
    CACGCCTCCT CCTGCAGTGA GAAGTCCCAT CGTGTGGACC CGCAGGTCAA AGTCAAACGC
    CACGCCTCCA GTGCCAATCA ATACAAGTAT GGCAAGAACC GAGCCGAGGA GGATGCCCGG
    AGGTACTTGG TAGAAAAAGA GAAGCTGGAG AAAGAGAAAG AGACGATTCG GACAGAGCTG
    ATAGCACTGA GACAGGAGAA GAGGGAACTG AAGGAAGCCA TTCGGAGCAG CCCAGGAGCA
    AAATTAAAGG CTCTGGAAGA AGCCGTGGCC ACCCTGGAAG CTCAGTGTCG GGCAAAGGAG
    GAGCGCCGGA TTGACCTGGA GCTGAAGCTG GTGGCTGTGA AGGAGCGCTT GCAGCAGTCC
    CTGGCAGGAG GGCCAGCCCT GGGGCTCTCC GTGAGCAGCA AGCCCAAGAG TGGGGAAACT
    GCAAATAAAC CCCAGAACAG CGTTCCAGAG CAACCTCTCC CTGTCAACTG TGTTTCTGAG
    CTGAGGAAGA GGAGCCCATC CATCGTAGCC TCCAACCAAG GAAGGGTGCT ACAGAAAGCC
    AAGGAATGGG AAATGAAGAA GACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12430
    Clone ID Related Accession (Same CDS sequence) NM_152406.2 , NM_152406.3 , NM_152406.4
    Accession Version NM_152406.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-13
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1-like 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA792192.1, BC125093.1, AK074185.1, AC131025.1 and BX107758.1. On Apr 16, 2016 this sequence version replaced NM_152406.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK074185.1, SRR1803613.172483.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACCGAG GCCAGGTGCT GGAGCAGCTG CTCCCAGAGC TCACCGGGCT GCTCAGCCTC 
    CTGGACCACG AGTACCTCAG CGATACCACC CTGGAAAAGA AGATGGCCGT GGCCTCCATC
    CTGCAGAGCC TGCAGCCCCT TCCAGCAAAG GAGGTCTCCT ACCTGTATGT GAACACAGCA
    GACCTCCACT CGGGGCCCAG CTTCGTGGAA TCCCTCTTTG AAGAATTTGA CTGTGACCTG
    AGTGACCTTC GGGACATGCC AGAGGATGAT GGGGAGCCCA GCAAAGGAGC CAGCCCTGAG
    CTAGCCAAGA GCCCACGCCT GAGAAACGCG GCCGACCTGC CTCCACCGCT CCCCAACAAG
    CCTCCCCCTG AGGACTACTA TGAAGAGGCC CTTCCTCTGG GACCCGGCAA GTCGCCTGAG
    TACATCAGCT CCCACAATGG CTGCAGCCCC TCACACTCGA TTGTGGATGG CTACTATGAG
    GACGCAGACA GCAGCTACCC TGCAACCAGG GTGAACGGCG AGCTTAAGAG CTCCTATAAT
    GACTCTGACG CAATGAGCAG CTCCTATGAG TCCTACGATG AAGAGGAGGA GGAAGGGAAG
    AGCCCGCAGC CCCGACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAA
    TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGTTTCG GGCAGTGGGC CAAGCAGCTG
    ACGGTCATCA GGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGATCG GCAGCCACAT
    CTGAGGTTGG CACTGGATAC CTGCAGCATC ATCTACGTGC CCAAGGACAG CCGGCACAAG
    AGGCACGAGC TGCGTTTCAC CCAGGGGGCT ACCGAGGTCT TGGTGCTGGC ACTGCAGAGC
    CGAGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAAG TGAGCAAGCC AGTTGGGGGA
    GCTGAGGGAG TGGAGGTCCC CAGATCCCCA GTCCTCCTGT GCAAGTTGGA CCTGGACAAG
    AGGCTGTCCC AAGAGAAGCA GACCTCAGAT TCTGACAGCG TGGGTGTGGG TGACAACTGT
    TCTACCCTTG GCCGCCGGGA GACCTGTGAT CACGGCAAAG GGAAGAAGAG CAGCCTGGCA
    GAACTGAAGG GCTCAATGAG CAGGGCTGCG GGCCGCAAGA TCACCCGTAT CATTGGCTTC
    TCCAAGAAGA AGACACTGGC CGATGACCTG CAGACGTCCT CCACCGAGGA GGAGGTTCCC
    TGCTGTGGCT ACCTGAACGT GCTGGTGAAC CAGGGCTGGA AGGAACGCTG GTGCCGCCTG
    AAGTGCAACA CTCTGTATTT CCACAAGGAT CACATGGACC TGCGAACCCA TGTGAACGCC
    ATCGCCCTGC AAGGCTGTGA GGTGGCCCCG GGCTTTGGGC CCCGACACCC ATTTGCCTTC
    AGGATCCTGC GCAACCGGCA GGAGGTGGCC ATCTTGGAGG CAAGCTGTTC AGAGGACATG
    GGTCGCTGGC TCGGGCTGCT GCTGGTGGAG ATGGGCTCCA GAGTCACTCC GGAGGCGCTG
    CACTATGACT ACGTGGATGT GGAGACCTTA ACCAGCATCG TCAGTGCTGG GCGCAACTCC
    TTCCTATATG CAAGATCCTG CCAGAATCAG TGGCCTGAGC CCCGAGTCTA TGATGATGTT
    CCTTATGAAA AGATGCAGGA CGAGGAGCCC GAGCGCCCCA CAGGGGCCCA GGTGAAGCGT
    CACGCCTCCT CCTGCAGTGA GAAGTCCCAT CGTGTGGACC CGCAGGTCAA AGTCAAACGC
    CACGCCTCCA GTGCCAATCA ATACAAGTAT GGCAAGAACC GAGCCGAGGA GGATGCCCGG
    AGGTACTTGG TAGAAAAAGA GAAGCTGGAG AAAGAGAAAG AGACGATTCG GACAGAGCTG
    ATAGCACTGA GACAGGAGAA GAGGGAACTG AAGGAAGCCA TTCGGAGCAG CCCAGGAGCA
    AAATTAAAGG CTCTGGAAGA AGCCGTGGCC ACCCTGGAAG CTCAGTGTCG GGCAAAGGAG
    GAGCGCCGGA TTGACCTGGA GCTGAAGCTG GTGGCTGTGA AGGAGCGCTT GCAGCAGTCC
    CTGGCAGGAG GGCCAGCCCT GGGGCTCTCC GTGAGCAGCA AGCCCAAGAG TGGGGAAACT
    GCAAATAAAC CCCAGAACAG CGTTCCAGAG CAACCTCTCC CTGTCAACTG TGTTTCTGAG
    CTGAGGAAGA GGAGCCCATC CATCGTAGCC TCCAACCAAG GAAGGGTGCT ACAGAAAGCC
    AAGGAATGGG AAATGAAGAA GACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_689619.1
    CDS132..2438
    Translation

    Target ORF information:

    RefSeq Version NM_152406.3
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_152406.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACCGAG GCCAGGTGCT GGAGCAGCTG CTCCCAGAGC TCACCGGGCT GCTCAGCCTC 
    CTGGACCACG AGTACCTCAG CGATACCACC CTGGAAAAGA AGATGGCCGT GGCCTCCATC
    CTGCAGAGCC TGCAGCCCCT TCCAGCAAAG GAGGTCTCCT ACCTGTATGT GAACACAGCA
    GACCTCCACT CGGGGCCCAG CTTCGTGGAA TCCCTCTTTG AAGAATTTGA CTGTGACCTG
    AGTGACCTTC GGGACATGCC AGAGGATGAT GGGGAGCCCA GCAAAGGAGC CAGCCCTGAG
    CTAGCCAAGA GCCCACGCCT GAGAAACGCG GCCGACCTGC CTCCACCGCT CCCCAACAAG
    CCTCCCCCTG AGGACTACTA TGAAGAGGCC CTTCCTCTGG GACCCGGCAA GTCGCCTGAG
    TACATCAGCT CCCACAATGG CTGCAGCCCC TCACACTCGA TTGTGGATGG CTACTATGAG
    GACGCAGACA GCAGCTACCC TGCAACCAGG GTGAACGGCG AGCTTAAGAG CTCCTATAAT
    GACTCTGACG CAATGAGCAG CTCCTATGAG TCCTACGATG AAGAGGAGGA GGAAGGGAAG
    AGCCCGCAGC CCCGACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAA
    TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGTTTCG GGCAGTGGGC CAAGCAGCTG
    ACGGTCATCA GGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGATCG GCAGCCACAT
    CTGAGGTTGG CACTGGATAC CTGCAGCATC ATCTACGTGC CCAAGGACAG CCGGCACAAG
    AGGCACGAGC TGCGTTTCAC CCAGGGGGCT ACCGAGGTCT TGGTGCTGGC ACTGCAGAGC
    CGAGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAAG TGAGCAAGCC AGTTGGGGGA
    GCTGAGGGAG TGGAGGTCCC CAGATCCCCA GTCCTCCTGT GCAAGTTGGA CCTGGACAAG
    AGGCTGTCCC AAGAGAAGCA GACCTCAGAT TCTGACAGCG TGGGTGTGGG TGACAACTGT
    TCTACCCTTG GCCGCCGGGA GACCTGTGAT CACGGCAAAG GGAAGAAGAG CAGCCTGGCA
    GAACTGAAGG GCTCAATGAG CAGGGCTGCG GGCCGCAAGA TCACCCGTAT CATTGGCTTC
    TCCAAGAAGA AGACACTGGC CGATGACCTG CAGACGTCCT CCACCGAGGA GGAGGTTCCC
    TGCTGTGGCT ACCTGAACGT GCTGGTGAAC CAGGGCTGGA AGGAACGCTG GTGCCGCCTG
    AAGTGCAACA CTCTGTATTT CCACAAGGAT CACATGGACC TGCGAACCCA TGTGAACGCC
    ATCGCCCTGC AAGGCTGTGA GGTGGCCCCG GGCTTTGGGC CCCGACACCC ATTTGCCTTC
    AGGATCCTGC GCAACCGGCA GGAGGTGGCC ATCTTGGAGG CAAGCTGTTC AGAGGACATG
    GGTCGCTGGC TCGGGCTGCT GCTGGTGGAG ATGGGCTCCA GAGTCACTCC GGAGGCGCTG
    CACTATGACT ACGTGGATGT GGAGACCTTA ACCAGCATCG TCAGTGCTGG GCGCAACTCC
    TTCCTATATG CAAGATCCTG CCAGAATCAG TGGCCTGAGC CCCGAGTCTA TGATGATGTT
    CCTTATGAAA AGATGCAGGA CGAGGAGCCC GAGCGCCCCA CAGGGGCCCA GGTGAAGCGT
    CACGCCTCCT CCTGCAGTGA GAAGTCCCAT CGTGTGGACC CGCAGGTCAA AGTCAAACGC
    CACGCCTCCA GTGCCAATCA ATACAAGTAT GGCAAGAACC GAGCCGAGGA GGATGCCCGG
    AGGTACTTGG TAGAAAAAGA GAAGCTGGAG AAAGAGAAAG AGACGATTCG GACAGAGCTG
    ATAGCACTGA GACAGGAGAA GAGGGAACTG AAGGAAGCCA TTCGGAGCAG CCCAGGAGCA
    AAATTAAAGG CTCTGGAAGA AGCCGTGGCC ACCCTGGAAG CTCAGTGTCG GGCAAAGGAG
    GAGCGCCGGA TTGACCTGGA GCTGAAGCTG GTGGCTGTGA AGGAGCGCTT GCAGCAGTCC
    CTGGCAGGAG GGCCAGCCCT GGGGCTCTCC GTGAGCAGCA AGCCCAAGAG TGGGGAAACT
    GCAAATAAAC CCCAGAACAG CGTTCCAGAG CAACCTCTCC CTGTCAACTG TGTTTCTGAG
    CTGAGGAAGA GGAGCCCATC CATCGTAGCC TCCAACCAAG GAAGGGTGCT ACAGAAAGCC
    AAGGAATGGG AAATGAAGAA GACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12430
    Clone ID Related Accession (Same CDS sequence) NM_152406.2 , NM_152406.3 , NM_152406.4
    Accession Version NM_152406.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1-like 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA792192.1, BC125093.1, AK074185.1, AC131025.1 and BX107758.1. On Nov 23, 2018 this sequence version replaced NM_152406.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK074185.1, SRR1803613.172483.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000296721.9/ ENSP00000296721.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACCGAG GCCAGGTGCT GGAGCAGCTG CTCCCAGAGC TCACCGGGCT GCTCAGCCTC 
    CTGGACCACG AGTACCTCAG CGATACCACC CTGGAAAAGA AGATGGCCGT GGCCTCCATC
    CTGCAGAGCC TGCAGCCCCT TCCAGCAAAG GAGGTCTCCT ACCTGTATGT GAACACAGCA
    GACCTCCACT CGGGGCCCAG CTTCGTGGAA TCCCTCTTTG AAGAATTTGA CTGTGACCTG
    AGTGACCTTC GGGACATGCC AGAGGATGAT GGGGAGCCCA GCAAAGGAGC CAGCCCTGAG
    CTAGCCAAGA GCCCACGCCT GAGAAACGCG GCCGACCTGC CTCCACCGCT CCCCAACAAG
    CCTCCCCCTG AGGACTACTA TGAAGAGGCC CTTCCTCTGG GACCCGGCAA GTCGCCTGAG
    TACATCAGCT CCCACAATGG CTGCAGCCCC TCACACTCGA TTGTGGATGG CTACTATGAG
    GACGCAGACA GCAGCTACCC TGCAACCAGG GTGAACGGCG AGCTTAAGAG CTCCTATAAT
    GACTCTGACG CAATGAGCAG CTCCTATGAG TCCTACGATG AAGAGGAGGA GGAAGGGAAG
    AGCCCGCAGC CCCGACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAA
    TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGTTTCG GGCAGTGGGC CAAGCAGCTG
    ACGGTCATCA GGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGATCG GCAGCCACAT
    CTGAGGTTGG CACTGGATAC CTGCAGCATC ATCTACGTGC CCAAGGACAG CCGGCACAAG
    AGGCACGAGC TGCGTTTCAC CCAGGGGGCT ACCGAGGTCT TGGTGCTGGC ACTGCAGAGC
    CGAGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAAG TGAGCAAGCC AGTTGGGGGA
    GCTGAGGGAG TGGAGGTCCC CAGATCCCCA GTCCTCCTGT GCAAGTTGGA CCTGGACAAG
    AGGCTGTCCC AAGAGAAGCA GACCTCAGAT TCTGACAGCG TGGGTGTGGG TGACAACTGT
    TCTACCCTTG GCCGCCGGGA GACCTGTGAT CACGGCAAAG GGAAGAAGAG CAGCCTGGCA
    GAACTGAAGG GCTCAATGAG CAGGGCTGCG GGCCGCAAGA TCACCCGTAT CATTGGCTTC
    TCCAAGAAGA AGACACTGGC CGATGACCTG CAGACGTCCT CCACCGAGGA GGAGGTTCCC
    TGCTGTGGCT ACCTGAACGT GCTGGTGAAC CAGGGCTGGA AGGAACGCTG GTGCCGCCTG
    AAGTGCAACA CTCTGTATTT CCACAAGGAT CACATGGACC TGCGAACCCA TGTGAACGCC
    ATCGCCCTGC AAGGCTGTGA GGTGGCCCCG GGCTTTGGGC CCCGACACCC ATTTGCCTTC
    AGGATCCTGC GCAACCGGCA GGAGGTGGCC ATCTTGGAGG CAAGCTGTTC AGAGGACATG
    GGTCGCTGGC TCGGGCTGCT GCTGGTGGAG ATGGGCTCCA GAGTCACTCC GGAGGCGCTG
    CACTATGACT ACGTGGATGT GGAGACCTTA ACCAGCATCG TCAGTGCTGG GCGCAACTCC
    TTCCTATATG CAAGATCCTG CCAGAATCAG TGGCCTGAGC CCCGAGTCTA TGATGATGTT
    CCTTATGAAA AGATGCAGGA CGAGGAGCCC GAGCGCCCCA CAGGGGCCCA GGTGAAGCGT
    CACGCCTCCT CCTGCAGTGA GAAGTCCCAT CGTGTGGACC CGCAGGTCAA AGTCAAACGC
    CACGCCTCCA GTGCCAATCA ATACAAGTAT GGCAAGAACC GAGCCGAGGA GGATGCCCGG
    AGGTACTTGG TAGAAAAAGA GAAGCTGGAG AAAGAGAAAG AGACGATTCG GACAGAGCTG
    ATAGCACTGA GACAGGAGAA GAGGGAACTG AAGGAAGCCA TTCGGAGCAG CCCAGGAGCA
    AAATTAAAGG CTCTGGAAGA AGCCGTGGCC ACCCTGGAAG CTCAGTGTCG GGCAAAGGAG
    GAGCGCCGGA TTGACCTGGA GCTGAAGCTG GTGGCTGTGA AGGAGCGCTT GCAGCAGTCC
    CTGGCAGGAG GGCCAGCCCT GGGGCTCTCC GTGAGCAGCA AGCCCAAGAG TGGGGAAACT
    GCAAATAAAC CCCAGAACAG CGTTCCAGAG CAACCTCTCC CTGTCAACTG TGTTTCTGAG
    CTGAGGAAGA GGAGCCCATC CATCGTAGCC TCCAACCAAG GAAGGGTGCT ACAGAAAGCC
    AAGGAATGGG AAATGAAGAA GACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_689619.1
    CDS111..2417
    Translation

    Target ORF information:

    RefSeq Version NM_152406.4
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_152406.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACCGAG GCCAGGTGCT GGAGCAGCTG CTCCCAGAGC TCACCGGGCT GCTCAGCCTC 
    CTGGACCACG AGTACCTCAG CGATACCACC CTGGAAAAGA AGATGGCCGT GGCCTCCATC
    CTGCAGAGCC TGCAGCCCCT TCCAGCAAAG GAGGTCTCCT ACCTGTATGT GAACACAGCA
    GACCTCCACT CGGGGCCCAG CTTCGTGGAA TCCCTCTTTG AAGAATTTGA CTGTGACCTG
    AGTGACCTTC GGGACATGCC AGAGGATGAT GGGGAGCCCA GCAAAGGAGC CAGCCCTGAG
    CTAGCCAAGA GCCCACGCCT GAGAAACGCG GCCGACCTGC CTCCACCGCT CCCCAACAAG
    CCTCCCCCTG AGGACTACTA TGAAGAGGCC CTTCCTCTGG GACCCGGCAA GTCGCCTGAG
    TACATCAGCT CCCACAATGG CTGCAGCCCC TCACACTCGA TTGTGGATGG CTACTATGAG
    GACGCAGACA GCAGCTACCC TGCAACCAGG GTGAACGGCG AGCTTAAGAG CTCCTATAAT
    GACTCTGACG CAATGAGCAG CTCCTATGAG TCCTACGATG AAGAGGAGGA GGAAGGGAAG
    AGCCCGCAGC CCCGACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAA
    TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGTTTCG GGCAGTGGGC CAAGCAGCTG
    ACGGTCATCA GGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGATCG GCAGCCACAT
    CTGAGGTTGG CACTGGATAC CTGCAGCATC ATCTACGTGC CCAAGGACAG CCGGCACAAG
    AGGCACGAGC TGCGTTTCAC CCAGGGGGCT ACCGAGGTCT TGGTGCTGGC ACTGCAGAGC
    CGAGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAAG TGAGCAAGCC AGTTGGGGGA
    GCTGAGGGAG TGGAGGTCCC CAGATCCCCA GTCCTCCTGT GCAAGTTGGA CCTGGACAAG
    AGGCTGTCCC AAGAGAAGCA GACCTCAGAT TCTGACAGCG TGGGTGTGGG TGACAACTGT
    TCTACCCTTG GCCGCCGGGA GACCTGTGAT CACGGCAAAG GGAAGAAGAG CAGCCTGGCA
    GAACTGAAGG GCTCAATGAG CAGGGCTGCG GGCCGCAAGA TCACCCGTAT CATTGGCTTC
    TCCAAGAAGA AGACACTGGC CGATGACCTG CAGACGTCCT CCACCGAGGA GGAGGTTCCC
    TGCTGTGGCT ACCTGAACGT GCTGGTGAAC CAGGGCTGGA AGGAACGCTG GTGCCGCCTG
    AAGTGCAACA CTCTGTATTT CCACAAGGAT CACATGGACC TGCGAACCCA TGTGAACGCC
    ATCGCCCTGC AAGGCTGTGA GGTGGCCCCG GGCTTTGGGC CCCGACACCC ATTTGCCTTC
    AGGATCCTGC GCAACCGGCA GGAGGTGGCC ATCTTGGAGG CAAGCTGTTC AGAGGACATG
    GGTCGCTGGC TCGGGCTGCT GCTGGTGGAG ATGGGCTCCA GAGTCACTCC GGAGGCGCTG
    CACTATGACT ACGTGGATGT GGAGACCTTA ACCAGCATCG TCAGTGCTGG GCGCAACTCC
    TTCCTATATG CAAGATCCTG CCAGAATCAG TGGCCTGAGC CCCGAGTCTA TGATGATGTT
    CCTTATGAAA AGATGCAGGA CGAGGAGCCC GAGCGCCCCA CAGGGGCCCA GGTGAAGCGT
    CACGCCTCCT CCTGCAGTGA GAAGTCCCAT CGTGTGGACC CGCAGGTCAA AGTCAAACGC
    CACGCCTCCA GTGCCAATCA ATACAAGTAT GGCAAGAACC GAGCCGAGGA GGATGCCCGG
    AGGTACTTGG TAGAAAAAGA GAAGCTGGAG AAAGAGAAAG AGACGATTCG GACAGAGCTG
    ATAGCACTGA GACAGGAGAA GAGGGAACTG AAGGAAGCCA TTCGGAGCAG CCCAGGAGCA
    AAATTAAAGG CTCTGGAAGA AGCCGTGGCC ACCCTGGAAG CTCAGTGTCG GGCAAAGGAG
    GAGCGCCGGA TTGACCTGGA GCTGAAGCTG GTGGCTGTGA AGGAGCGCTT GCAGCAGTCC
    CTGGCAGGAG GGCCAGCCCT GGGGCTCTCC GTGAGCAGCA AGCCCAAGAG TGGGGAAACT
    GCAAATAAAC CCCAGAACAG CGTTCCAGAG CAACCTCTCC CTGTCAACTG TGTTTCTGAG
    CTGAGGAAGA GGAGCCCATC CATCGTAGCC TCCAACCAAG GAAGGGTGCT ACAGAAAGCC
    AAGGAATGGG AAATGAAGAA GACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu75735
    Clone ID Related Accession (Same CDS sequence) XM_011537558.2 , XM_011537558.1
    Accession Version XM_011537558.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1887bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1-like 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011537558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGTGCTTG GATCAGATGG CTGCAGCCCC TCACACTCGA TTGTGGATGG CTACTATGAG 
    GACGCAGACA GCAGCTACCC TGCAACCAGG GTGAACGGCG AGCTTAAGAG CTCCTATAAT
    GACTCTGACG CAATGAGCAG CTCCTATGAG TCCTACGATG AAGAGGAGGA GGAAGGGAAG
    AGCCCGCAGC CCCGACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAA
    TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGTTTCG GGCAGTGGGC CAAGCAGCTG
    ACGGTCATCA GGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGATCG GCAGCCACAT
    CTGAGGTTGG CACTGGATAC CTGCAGCATC ATCTACGTGC CCAAGGACAG CCGGCACAAG
    AGGCACGAGC TGCGTTTCAC CCAGGGGGCT ACCGAGGTCT TGGTGCTGGC ACTGCAGAGC
    CGAGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAAG TGAGCAAGCC AGTTGGGGGA
    GCTGAGGGAG TGGAGGTCCC CAGATCCCCA GTCCTCCTGT GCAAGTTGGA CCTGGACAAG
    AGGCTGTCCC AAGAGAAGCA GACCTCAGAT TCTGACAGCG TGGGTGTGGG TGACAACTGT
    TCTACCCTTG GCCGCCGGGA GACCTGTGAT CACGGCAAAG GGAAGAAGAG CAGCCTGGCA
    GAACTGAAGG GCTCAATGAG CAGGGCTGCG GGCCGCAAGA TCACCCGTAT CATTGGCTTC
    TCCAAGAAGA AGACACTGGC CGATGACCTG CAGACGTCCT CCACCGAGGA GGAGGTTCCC
    TGCTGTGGCT ACCTGAACGT GCTGGTGAAC CAGGGCTGGA AGGAACGCTG GTGCCGCCTG
    AAGTGCAACA CTCTGTATTT CCACAAGGAT CACATGGACC TGCGAACCCA TGTGAACGCC
    ATCGCCCTGC AAGGCTGTGA GGTGGCCCCG GGCTTTGGGC CCCGACACCC ATTTGCCTTC
    AGGATCCTGC GCAACCGGCA GGAGGTGGCC ATCTTGGAGG CAAGCTGTTC AGAGGACATG
    GGTCGCTGGC TCGGGCTGCT GCTGGTGGAG ATGGGCTCCA GAGTCACTCC GGAGGCGCTG
    CACTATGACT ACGTGGATGT GGAGACCTTA ACCAGCATCG TCAGTGCTGG GCGCAACTCC
    TTCCTATATG CAAGATCCTG CCAGAATCAG TGGCCTGAGC CCCGAGTCTA TGATGATGTT
    CCTTATGAAA AGATGCAGGA CGAGGAGCCC GAGCGCCCCA CAGGGGCCCA GGTGAAGCGT
    CACGCCTCCT CCTGCAGTGA GAAGTCCCAT CGTGTGGACC CGCAGGTCAA AGTCAAACGC
    CACGCCTCCA GTGCCAATCA ATACAAGTAT GGCAAGAACC GAGCCGAGGA GGATGCCCGG
    AGGTACTTGG TAGAAAAAGA GAAGCTGGAG AAAGAGAAAG AGACGATTCG GACAGAGCTG
    ATAGCACTGA GACAGGAGAA GAGGGAACTG AAGGAAGCCA TTCGGAGCAG CCCAGGAGCA
    AAATTAAAGG CTCTGGAAGA AGCCGTGGCC ACCCTGGAAG CTCAGTGTCG GGCAAAGGAG
    GAGCGCCGGA TTGACCTGGA GCTGAAGCTG GTGGCTGTGA AGGAGCGCTT GCAGCAGTCC
    CTGGCAGGAG GGCCAGCCCT GGGGCTCTCC GTGAGCAGCA AGCCCAAGAG TGGGGAAACT
    GCAAATAAAC CCCAGAACAG CGTTCCAGAG CAACCTCTCC CTGTCAACTG TGTTTCTGAG
    CTGAGGAAGA GGAGCCCATC CATCGTAGCC TCCAACCAAG GAAGGGTGCT ACAGAAAGCC
    AAGGAATGGG AAATGAAGAA GACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011535860.1
    CDS111..1997
    Translation

    Target ORF information:

    RefSeq Version XM_011537558.2
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011537558.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGTGCTTG GATCAGATGG CTGCAGCCCC TCACACTCGA TTGTGGATGG CTACTATGAG 
    GACGCAGACA GCAGCTACCC TGCAACCAGG GTGAACGGCG AGCTTAAGAG CTCCTATAAT
    GACTCTGACG CAATGAGCAG CTCCTATGAG TCCTACGATG AAGAGGAGGA GGAAGGGAAG
    AGCCCGCAGC CCCGACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAA
    TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGTTTCG GGCAGTGGGC CAAGCAGCTG
    ACGGTCATCA GGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGATCG GCAGCCACAT
    CTGAGGTTGG CACTGGATAC CTGCAGCATC ATCTACGTGC CCAAGGACAG CCGGCACAAG
    AGGCACGAGC TGCGTTTCAC CCAGGGGGCT ACCGAGGTCT TGGTGCTGGC ACTGCAGAGC
    CGAGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAAG TGAGCAAGCC AGTTGGGGGA
    GCTGAGGGAG TGGAGGTCCC CAGATCCCCA GTCCTCCTGT GCAAGTTGGA CCTGGACAAG
    AGGCTGTCCC AAGAGAAGCA GACCTCAGAT TCTGACAGCG TGGGTGTGGG TGACAACTGT
    TCTACCCTTG GCCGCCGGGA GACCTGTGAT CACGGCAAAG GGAAGAAGAG CAGCCTGGCA
    GAACTGAAGG GCTCAATGAG CAGGGCTGCG GGCCGCAAGA TCACCCGTAT CATTGGCTTC
    TCCAAGAAGA AGACACTGGC CGATGACCTG CAGACGTCCT CCACCGAGGA GGAGGTTCCC
    TGCTGTGGCT ACCTGAACGT GCTGGTGAAC CAGGGCTGGA AGGAACGCTG GTGCCGCCTG
    AAGTGCAACA CTCTGTATTT CCACAAGGAT CACATGGACC TGCGAACCCA TGTGAACGCC
    ATCGCCCTGC AAGGCTGTGA GGTGGCCCCG GGCTTTGGGC CCCGACACCC ATTTGCCTTC
    AGGATCCTGC GCAACCGGCA GGAGGTGGCC ATCTTGGAGG CAAGCTGTTC AGAGGACATG
    GGTCGCTGGC TCGGGCTGCT GCTGGTGGAG ATGGGCTCCA GAGTCACTCC GGAGGCGCTG
    CACTATGACT ACGTGGATGT GGAGACCTTA ACCAGCATCG TCAGTGCTGG GCGCAACTCC
    TTCCTATATG CAAGATCCTG CCAGAATCAG TGGCCTGAGC CCCGAGTCTA TGATGATGTT
    CCTTATGAAA AGATGCAGGA CGAGGAGCCC GAGCGCCCCA CAGGGGCCCA GGTGAAGCGT
    CACGCCTCCT CCTGCAGTGA GAAGTCCCAT CGTGTGGACC CGCAGGTCAA AGTCAAACGC
    CACGCCTCCA GTGCCAATCA ATACAAGTAT GGCAAGAACC GAGCCGAGGA GGATGCCCGG
    AGGTACTTGG TAGAAAAAGA GAAGCTGGAG AAAGAGAAAG AGACGATTCG GACAGAGCTG
    ATAGCACTGA GACAGGAGAA GAGGGAACTG AAGGAAGCCA TTCGGAGCAG CCCAGGAGCA
    AAATTAAAGG CTCTGGAAGA AGCCGTGGCC ACCCTGGAAG CTCAGTGTCG GGCAAAGGAG
    GAGCGCCGGA TTGACCTGGA GCTGAAGCTG GTGGCTGTGA AGGAGCGCTT GCAGCAGTCC
    CTGGCAGGAG GGCCAGCCCT GGGGCTCTCC GTGAGCAGCA AGCCCAAGAG TGGGGAAACT
    GCAAATAAAC CCCAGAACAG CGTTCCAGAG CAACCTCTCC CTGTCAACTG TGTTTCTGAG
    CTGAGGAAGA GGAGCCCATC CATCGTAGCC TCCAACCAAG GAAGGGTGCT ACAGAAAGCC
    AAGGAATGGG AAATGAAGAA GACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu75735
    Clone ID Related Accession (Same CDS sequence) XM_011537558.2 , XM_011537558.1
    Accession Version XM_011537558.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1887bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1-like 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGTGCTTG GATCAGATGG CTGCAGCCCC TCACACTCGA TTGTGGATGG CTACTATGAG 
    GACGCAGACA GCAGCTACCC TGCAACCAGG GTGAACGGCG AGCTTAAGAG CTCCTATAAT
    GACTCTGACG CAATGAGCAG CTCCTATGAG TCCTACGATG AAGAGGAGGA GGAAGGGAAG
    AGCCCGCAGC CCCGACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAA
    TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGTTTCG GGCAGTGGGC CAAGCAGCTG
    ACGGTCATCA GGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGATCG GCAGCCACAT
    CTGAGGTTGG CACTGGATAC CTGCAGCATC ATCTACGTGC CCAAGGACAG CCGGCACAAG
    AGGCACGAGC TGCGTTTCAC CCAGGGGGCT ACCGAGGTCT TGGTGCTGGC ACTGCAGAGC
    CGAGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAAG TGAGCAAGCC AGTTGGGGGA
    GCTGAGGGAG TGGAGGTCCC CAGATCCCCA GTCCTCCTGT GCAAGTTGGA CCTGGACAAG
    AGGCTGTCCC AAGAGAAGCA GACCTCAGAT TCTGACAGCG TGGGTGTGGG TGACAACTGT
    TCTACCCTTG GCCGCCGGGA GACCTGTGAT CACGGCAAAG GGAAGAAGAG CAGCCTGGCA
    GAACTGAAGG GCTCAATGAG CAGGGCTGCG GGCCGCAAGA TCACCCGTAT CATTGGCTTC
    TCCAAGAAGA AGACACTGGC CGATGACCTG CAGACGTCCT CCACCGAGGA GGAGGTTCCC
    TGCTGTGGCT ACCTGAACGT GCTGGTGAAC CAGGGCTGGA AGGAACGCTG GTGCCGCCTG
    AAGTGCAACA CTCTGTATTT CCACAAGGAT CACATGGACC TGCGAACCCA TGTGAACGCC
    ATCGCCCTGC AAGGCTGTGA GGTGGCCCCG GGCTTTGGGC CCCGACACCC ATTTGCCTTC
    AGGATCCTGC GCAACCGGCA GGAGGTGGCC ATCTTGGAGG CAAGCTGTTC AGAGGACATG
    GGTCGCTGGC TCGGGCTGCT GCTGGTGGAG ATGGGCTCCA GAGTCACTCC GGAGGCGCTG
    CACTATGACT ACGTGGATGT GGAGACCTTA ACCAGCATCG TCAGTGCTGG GCGCAACTCC
    TTCCTATATG CAAGATCCTG CCAGAATCAG TGGCCTGAGC CCCGAGTCTA TGATGATGTT
    CCTTATGAAA AGATGCAGGA CGAGGAGCCC GAGCGCCCCA CAGGGGCCCA GGTGAAGCGT
    CACGCCTCCT CCTGCAGTGA GAAGTCCCAT CGTGTGGACC CGCAGGTCAA AGTCAAACGC
    CACGCCTCCA GTGCCAATCA ATACAAGTAT GGCAAGAACC GAGCCGAGGA GGATGCCCGG
    AGGTACTTGG TAGAAAAAGA GAAGCTGGAG AAAGAGAAAG AGACGATTCG GACAGAGCTG
    ATAGCACTGA GACAGGAGAA GAGGGAACTG AAGGAAGCCA TTCGGAGCAG CCCAGGAGCA
    AAATTAAAGG CTCTGGAAGA AGCCGTGGCC ACCCTGGAAG CTCAGTGTCG GGCAAAGGAG
    GAGCGCCGGA TTGACCTGGA GCTGAAGCTG GTGGCTGTGA AGGAGCGCTT GCAGCAGTCC
    CTGGCAGGAG GGCCAGCCCT GGGGCTCTCC GTGAGCAGCA AGCCCAAGAG TGGGGAAACT
    GCAAATAAAC CCCAGAACAG CGTTCCAGAG CAACCTCTCC CTGTCAACTG TGTTTCTGAG
    CTGAGGAAGA GGAGCCCATC CATCGTAGCC TCCAACCAAG GAAGGGTGCT ACAGAAAGCC
    AAGGAATGGG AAATGAAGAA GACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011535860.1
    CDS102..1988
    Translation

    Target ORF information:

    RefSeq Version XM_011537558.1
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011537558.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGTGCTTG GATCAGATGG CTGCAGCCCC TCACACTCGA TTGTGGATGG CTACTATGAG 
    GACGCAGACA GCAGCTACCC TGCAACCAGG GTGAACGGCG AGCTTAAGAG CTCCTATAAT
    GACTCTGACG CAATGAGCAG CTCCTATGAG TCCTACGATG AAGAGGAGGA GGAAGGGAAG
    AGCCCGCAGC CCCGACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAA
    TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGTTTCG GGCAGTGGGC CAAGCAGCTG
    ACGGTCATCA GGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGATCG GCAGCCACAT
    CTGAGGTTGG CACTGGATAC CTGCAGCATC ATCTACGTGC CCAAGGACAG CCGGCACAAG
    AGGCACGAGC TGCGTTTCAC CCAGGGGGCT ACCGAGGTCT TGGTGCTGGC ACTGCAGAGC
    CGAGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAAG TGAGCAAGCC AGTTGGGGGA
    GCTGAGGGAG TGGAGGTCCC CAGATCCCCA GTCCTCCTGT GCAAGTTGGA CCTGGACAAG
    AGGCTGTCCC AAGAGAAGCA GACCTCAGAT TCTGACAGCG TGGGTGTGGG TGACAACTGT
    TCTACCCTTG GCCGCCGGGA GACCTGTGAT CACGGCAAAG GGAAGAAGAG CAGCCTGGCA
    GAACTGAAGG GCTCAATGAG CAGGGCTGCG GGCCGCAAGA TCACCCGTAT CATTGGCTTC
    TCCAAGAAGA AGACACTGGC CGATGACCTG CAGACGTCCT CCACCGAGGA GGAGGTTCCC
    TGCTGTGGCT ACCTGAACGT GCTGGTGAAC CAGGGCTGGA AGGAACGCTG GTGCCGCCTG
    AAGTGCAACA CTCTGTATTT CCACAAGGAT CACATGGACC TGCGAACCCA TGTGAACGCC
    ATCGCCCTGC AAGGCTGTGA GGTGGCCCCG GGCTTTGGGC CCCGACACCC ATTTGCCTTC
    AGGATCCTGC GCAACCGGCA GGAGGTGGCC ATCTTGGAGG CAAGCTGTTC AGAGGACATG
    GGTCGCTGGC TCGGGCTGCT GCTGGTGGAG ATGGGCTCCA GAGTCACTCC GGAGGCGCTG
    CACTATGACT ACGTGGATGT GGAGACCTTA ACCAGCATCG TCAGTGCTGG GCGCAACTCC
    TTCCTATATG CAAGATCCTG CCAGAATCAG TGGCCTGAGC CCCGAGTCTA TGATGATGTT
    CCTTATGAAA AGATGCAGGA CGAGGAGCCC GAGCGCCCCA CAGGGGCCCA GGTGAAGCGT
    CACGCCTCCT CCTGCAGTGA GAAGTCCCAT CGTGTGGACC CGCAGGTCAA AGTCAAACGC
    CACGCCTCCA GTGCCAATCA ATACAAGTAT GGCAAGAACC GAGCCGAGGA GGATGCCCGG
    AGGTACTTGG TAGAAAAAGA GAAGCTGGAG AAAGAGAAAG AGACGATTCG GACAGAGCTG
    ATAGCACTGA GACAGGAGAA GAGGGAACTG AAGGAAGCCA TTCGGAGCAG CCCAGGAGCA
    AAATTAAAGG CTCTGGAAGA AGCCGTGGCC ACCCTGGAAG CTCAGTGTCG GGCAAAGGAG
    GAGCGCCGGA TTGACCTGGA GCTGAAGCTG GTGGCTGTGA AGGAGCGCTT GCAGCAGTCC
    CTGGCAGGAG GGCCAGCCCT GGGGCTCTCC GTGAGCAGCA AGCCCAAGAG TGGGGAAACT
    GCAAATAAAC CCCAGAACAG CGTTCCAGAG CAACCTCTCC CTGTCAACTG TGTTTCTGAG
    CTGAGGAAGA GGAGCCCATC CATCGTAGCC TCCAACCAAG GAAGGGTGCT ACAGAAAGCC
    AAGGAATGGG AAATGAAGAA GACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100402
    Clone ID Related Accession (Same CDS sequence) NM_001323063.1
    Accession Version NM_001323063.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1134bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1-like 1 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA792192.1, BC040723.2 and AC131025.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC040723.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGACCGAG GCCAGGTGCT GGAGCAGCTG CTCCCAGAGC TCACCGGGCT GCTCAGCCTC 
    CTGGACCACG AGTACCTCAG CGATACCACC CTGGAAAAGA AGATGGCCGT GGCCTCCATC
    CTGCAGAGCC TGCAGCCCCT TCCAGCAAAG GAGGTCTCCT ACCTGTATGT GAACACAGCA
    GACCTCCACT CGGGGCCCAG CTTCGTGGAA TCCCTCTTTG AAGAATTTGA CTGTGACCTG
    AGTGACCTTC GGGACATGCC AGAGGATGAT GGGGAGCCCA GCAAAGGAGC CAGCCCTGAG
    CTAGCCAAGA GCCCACGCCT GAGAAACGCG GCCGACCTGC CTCCACCGCT CCCCAACAAG
    CCTCCCCCTG AGGACTACTA TGAAGAGGCC CTTCCTCTGG GACCCGGCAA GTCGCCTGAG
    TACATCAGCT CCCACAATGG CTGCAGCCCC TCACACTCGA TTGTGGATGG CTACTATGAG
    GACGCAGACA GCAGCTACCC TGCAACCAGG GTGAACGGCG AGCTTAAGAG CTCCTATAAT
    GACTCTGACG CAATGAGCAG CTCCTATGAG TCCTACGATG AAGAGGAGGA GGAAGGGAAG
    AGCCCGCAGC CCCGACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAA
    TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGTTTCG GGCAGTGGGC CAAGCAGCTG
    ACGGTCATCA GGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGATCG GCAGCCACAT
    CTGAGGTTGG CACTGGATAC CTGCAGCATC ATCTACGTGC CCAAGGACAG CCGGCACAAG
    AGGCACGAGC TGCGTTTCAC CCAGGGGGCT ACCGAGGTCT TGGTGCTGGC ACTGCAGAGC
    CGAGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAAG TGAGCAAGCC AGTTGGGGGA
    GCTGAGGGAG TGGAGGTCCC CAGATCCCCA GTCCTCCTGT GCAAGTTGGA CCTGGACAAG
    GTATATCTGT CTCCACTAAG TCTTCCCCAG GCCAGGCAGT GGCCACTCAA CACGGGCTCA
    ACCCCAGGGG AACTAACTGG CTGGGGGGAA AGTCAGGCAA CTGCCAAACT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001309992.1
    CDS132..1265
    Translation

    Target ORF information:

    RefSeq Version NM_001323063.1
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001323063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGACCGAG GCCAGGTGCT GGAGCAGCTG CTCCCAGAGC TCACCGGGCT GCTCAGCCTC 
    CTGGACCACG AGTACCTCAG CGATACCACC CTGGAAAAGA AGATGGCCGT GGCCTCCATC
    CTGCAGAGCC TGCAGCCCCT TCCAGCAAAG GAGGTCTCCT ACCTGTATGT GAACACAGCA
    GACCTCCACT CGGGGCCCAG CTTCGTGGAA TCCCTCTTTG AAGAATTTGA CTGTGACCTG
    AGTGACCTTC GGGACATGCC AGAGGATGAT GGGGAGCCCA GCAAAGGAGC CAGCCCTGAG
    CTAGCCAAGA GCCCACGCCT GAGAAACGCG GCCGACCTGC CTCCACCGCT CCCCAACAAG
    CCTCCCCCTG AGGACTACTA TGAAGAGGCC CTTCCTCTGG GACCCGGCAA GTCGCCTGAG
    TACATCAGCT CCCACAATGG CTGCAGCCCC TCACACTCGA TTGTGGATGG CTACTATGAG
    GACGCAGACA GCAGCTACCC TGCAACCAGG GTGAACGGCG AGCTTAAGAG CTCCTATAAT
    GACTCTGACG CAATGAGCAG CTCCTATGAG TCCTACGATG AAGAGGAGGA GGAAGGGAAG
    AGCCCGCAGC CCCGACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAA
    TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGTTTCG GGCAGTGGGC CAAGCAGCTG
    ACGGTCATCA GGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGATCG GCAGCCACAT
    CTGAGGTTGG CACTGGATAC CTGCAGCATC ATCTACGTGC CCAAGGACAG CCGGCACAAG
    AGGCACGAGC TGCGTTTCAC CCAGGGGGCT ACCGAGGTCT TGGTGCTGGC ACTGCAGAGC
    CGAGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAAG TGAGCAAGCC AGTTGGGGGA
    GCTGAGGGAG TGGAGGTCCC CAGATCCCCA GTCCTCCTGT GCAAGTTGGA CCTGGACAAG
    GTATATCTGT CTCCACTAAG TCTTCCCCAG GCCAGGCAGT GGCCACTCAA CACGGGCTCA
    ACCCCAGGGG AACTAACTGG CTGGGGGGAA AGTCAGGCAA CTGCCAAACT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95655
    Clone ID Related Accession (Same CDS sequence) XM_017009036.1
    Accession Version XM_017009036.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1-like 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCGTGG CCTCCATCCT GCAGAGCCTG CAGCCCCTTC CAGCAAAGGA GGTCTCCTAC 
    CTGTATGTGA ACACAGCAGA CCTCCACTCG GGGCCCAGCT TCGTGGAATC CCTCTTTGAA
    GAATTTGACT GTGACCTGAG TGACCTTCGG GACATGCCAG AGGATGATGG GGAGCCCAGC
    AAAGGAGCCA GCCCTGAGCT AGCCAAGAGC CCACGCCTGA GAAACGCGGC CGACCTGCCT
    CCACCGCTCC CCAACAAGCC TCCCCCTGAG GACTACTATG AAGAGGCCCT TCCTCTGGGA
    CCCGGCAAGT CGCCTGAGTA CATCAGCTCC CACAATGGCT GCAGCCCCTC ACACTCGATT
    GTGGATGGCT ACTATGAGGA CGCAGACAGC AGCTACCCTG CAACCAGGGT GAACGGCGAG
    CTTAAGAGCT CCTATAATGA CTCTGACGCA ATGAGCAGCT CCTATGAGTC CTACGATGAA
    GAGGAGGAGG AAGGGAAGAG CCCGCAGCCC CGACACCAGT GGCCCTCAGA GGAGGCCTCC
    ATGCACCTGG TGAGGGAATG CAGGATATGT GCCTTCCTGC TGCGGAAAAA GCGTTTCGGG
    CAGTGGGCCA AGCAGCTGAC GGTCATCAGG GAGGACCAGC TCCTGTGTTA CAAAAGCTCC
    AAGGATCGGC AGCCACATCT GAGGTTGGCA CTGGATACCT GCAGCATCAT CTACGTGCCC
    AAGGACAGCC GGCACAAGAG GCACGAGCTG CGTTTCACCC AGGGGGCTAC CGAGGTCTTG
    GTGCTGGCAC TGCAGAGCCG AGAGCAGGCC GAGGAGTGGC TGAAGGTCAT CCGAGAAGTG
    AGCAAGCCAG TTGGGGGAGC TGAGGGAGTG GAGGTCCCCA GATCCCCAGT CCTCCTGTGC
    AAGTTGGACC TGGACAAGAG GCTGTCCCAA GAGAAGCAGA CCTCAGATTC TGACAGCGTG
    GGTGTGGGTG ACAACTGTTC TACCCTTGGC CGCCGGGAGA CCTGTGATCA CGGCAAAGGG
    AAGAAGAGCA GCCTGGCAGA ACTGAAGGGC TCAATGAGCA GGGCTGCGGG CCGCAAGATC
    ACCCGTATCA TTGGCTTCTC CAAGAAGAAG ACACTGGCCG ATGACCTGCA GACGTCCTCC
    ACCGAGGAGG AGGTTCCCTG CTGTGGCTAC CTGAACGTGC TGGTGAACCA GGGCTGGAAG
    GAACGCTGGT GCCGCCTGAA GTGCAACACT CTGTATTTCC ACAAGGATCA CATGGACCTG
    CGAACCCATG TGAACGCCAT CGCCCTGCAA GGCTGTGAGG TGGCCCCGGG CTTTGGGCCC
    CGACACCCAT TTGCCTTCAG GATCCTGCGC AACCGGCAGG AGGTGGCCAT CTTGGAGGCA
    AGCTGTTCAG AGGACATGGG TCGCTGGCTC GGGCTGCTGC TGGTGGAGAT GGGCTCCAGA
    GTCACTCCGG AGGCGCTGCA CTATGACTAC GTGGATGTGG AGACCTTAAC CAGCATCGTC
    AGTGCTGGGC GCAACTCCTT CCTATATGCA AGATCCTGCC AGAATCAGTG GCCTGAGCCC
    CGAGTCTATG ATGATGTTCC TTATGAAAAG ATGCAGGACG AGGAGCCCGA GCGCCCCACA
    GGGGCCCAGG TGAAGCGTCA CGCCTCCTCC TGCAGTGAGA AGTCCCATCG TGTGGACCCG
    CAGGTCAAAG TCAAACGCCA CGCCTCCAGT GCCAATCAAT ACAAGTATGG CAAGAACCGA
    GCCGAGGAGG ATGCCCGGAG GTACTTGGTA GAAAAAGAGA AGCTGGAGAA AGAGAAAGAG
    ACGATTCGGA CAGAGCTGAT AGCACTGAGA CAGGAGAAGA GGGAACTGAA GGAAGCCATT
    CGGAGCAGCC CAGGAGCAAA ATTAAAGGCT CTGGAAGAAG CCGTGGCCAC CCTGGAAGCT
    CAGTGTCGGG CAAAGGAGGA GCGCCGGATT GACCTGGAGC TGAAGCTGGT GGCTGTGAAG
    GAGCGCTTGC AGCAGTCCCT GGCAGGAGGG CCAGCCCTGG GGCTCTCCGT GAGCAGCAAG
    CCCAAGAGTG GGGAAACTGC AAATAAACCC CAGAACAGCG TTCCAGAGCA ACCTCTCCCT
    GTCAACTGTG TTTCTGAGCT GAGGAAGAGG AGCCCATCCA TCGTAGCCTC CAACCAAGGA
    AGGGTGCTAC AGAAAGCCAA GGAATGGGAA ATGAAGAAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864525.1
    CDS211..2415
    Translation

    Target ORF information:

    RefSeq Version XM_017009036.1
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009036.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCGTGG CCTCCATCCT GCAGAGCCTG CAGCCCCTTC CAGCAAAGGA GGTCTCCTAC 
    CTGTATGTGA ACACAGCAGA CCTCCACTCG GGGCCCAGCT TCGTGGAATC CCTCTTTGAA
    GAATTTGACT GTGACCTGAG TGACCTTCGG GACATGCCAG AGGATGATGG GGAGCCCAGC
    AAAGGAGCCA GCCCTGAGCT AGCCAAGAGC CCACGCCTGA GAAACGCGGC CGACCTGCCT
    CCACCGCTCC CCAACAAGCC TCCCCCTGAG GACTACTATG AAGAGGCCCT TCCTCTGGGA
    CCCGGCAAGT CGCCTGAGTA CATCAGCTCC CACAATGGCT GCAGCCCCTC ACACTCGATT
    GTGGATGGCT ACTATGAGGA CGCAGACAGC AGCTACCCTG CAACCAGGGT GAACGGCGAG
    CTTAAGAGCT CCTATAATGA CTCTGACGCA ATGAGCAGCT CCTATGAGTC CTACGATGAA
    GAGGAGGAGG AAGGGAAGAG CCCGCAGCCC CGACACCAGT GGCCCTCAGA GGAGGCCTCC
    ATGCACCTGG TGAGGGAATG CAGGATATGT GCCTTCCTGC TGCGGAAAAA GCGTTTCGGG
    CAGTGGGCCA AGCAGCTGAC GGTCATCAGG GAGGACCAGC TCCTGTGTTA CAAAAGCTCC
    AAGGATCGGC AGCCACATCT GAGGTTGGCA CTGGATACCT GCAGCATCAT CTACGTGCCC
    AAGGACAGCC GGCACAAGAG GCACGAGCTG CGTTTCACCC AGGGGGCTAC CGAGGTCTTG
    GTGCTGGCAC TGCAGAGCCG AGAGCAGGCC GAGGAGTGGC TGAAGGTCAT CCGAGAAGTG
    AGCAAGCCAG TTGGGGGAGC TGAGGGAGTG GAGGTCCCCA GATCCCCAGT CCTCCTGTGC
    AAGTTGGACC TGGACAAGAG GCTGTCCCAA GAGAAGCAGA CCTCAGATTC TGACAGCGTG
    GGTGTGGGTG ACAACTGTTC TACCCTTGGC CGCCGGGAGA CCTGTGATCA CGGCAAAGGG
    AAGAAGAGCA GCCTGGCAGA ACTGAAGGGC TCAATGAGCA GGGCTGCGGG CCGCAAGATC
    ACCCGTATCA TTGGCTTCTC CAAGAAGAAG ACACTGGCCG ATGACCTGCA GACGTCCTCC
    ACCGAGGAGG AGGTTCCCTG CTGTGGCTAC CTGAACGTGC TGGTGAACCA GGGCTGGAAG
    GAACGCTGGT GCCGCCTGAA GTGCAACACT CTGTATTTCC ACAAGGATCA CATGGACCTG
    CGAACCCATG TGAACGCCAT CGCCCTGCAA GGCTGTGAGG TGGCCCCGGG CTTTGGGCCC
    CGACACCCAT TTGCCTTCAG GATCCTGCGC AACCGGCAGG AGGTGGCCAT CTTGGAGGCA
    AGCTGTTCAG AGGACATGGG TCGCTGGCTC GGGCTGCTGC TGGTGGAGAT GGGCTCCAGA
    GTCACTCCGG AGGCGCTGCA CTATGACTAC GTGGATGTGG AGACCTTAAC CAGCATCGTC
    AGTGCTGGGC GCAACTCCTT CCTATATGCA AGATCCTGCC AGAATCAGTG GCCTGAGCCC
    CGAGTCTATG ATGATGTTCC TTATGAAAAG ATGCAGGACG AGGAGCCCGA GCGCCCCACA
    GGGGCCCAGG TGAAGCGTCA CGCCTCCTCC TGCAGTGAGA AGTCCCATCG TGTGGACCCG
    CAGGTCAAAG TCAAACGCCA CGCCTCCAGT GCCAATCAAT ACAAGTATGG CAAGAACCGA
    GCCGAGGAGG ATGCCCGGAG GTACTTGGTA GAAAAAGAGA AGCTGGAGAA AGAGAAAGAG
    ACGATTCGGA CAGAGCTGAT AGCACTGAGA CAGGAGAAGA GGGAACTGAA GGAAGCCATT
    CGGAGCAGCC CAGGAGCAAA ATTAAAGGCT CTGGAAGAAG CCGTGGCCAC CCTGGAAGCT
    CAGTGTCGGG CAAAGGAGGA GCGCCGGATT GACCTGGAGC TGAAGCTGGT GGCTGTGAAG
    GAGCGCTTGC AGCAGTCCCT GGCAGGAGGG CCAGCCCTGG GGCTCTCCGT GAGCAGCAAG
    CCCAAGAGTG GGGAAACTGC AAATAAACCC CAGAACAGCG TTCCAGAGCA ACCTCTCCCT
    GTCAACTGTG TTTCTGAGCT GAGGAAGAGG AGCCCATCCA TCGTAGCCTC CAACCAAGGA
    AGGGTGCTAC AGAAAGCCAA GGAATGGGAA ATGAAGAAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11195
    Clone ID Related Accession (Same CDS sequence) NM_001146337.3 , NM_001146337.2
    Accession Version NM_001146337.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-08
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1-like 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA792192.1, BC125093.1, BC125094.1, AK074185.1, AC131025.1 and BX107758.1. On May 31, 2019 this sequence version replaced NM_001146337.2. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC125094.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153031, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGACCGAG GCCAGGTGCT GGAGCAGCTG CTCCCAGAGC TCACCGGGCT GCTCAGCCTC 
    CTGGACCACG AGTACCTCAG CGATACCACC CTGGAAAAGA AGATGGCCGT GGCCTCCATC
    CTGCAGAGCC TGCAGCCCCT TCCAGCAAAG GAGGTCTCCT ACCTGTATGT GAACACAGCA
    GACCTCCACT CGGGGCCCAG CTTCGTGGAA TCCCTCTTTG AAGAATTTGA CTGTGACCTG
    AGTGACCTTC GGGACATGCC AGAGGATGAT GGGGAGCCCA GCAAAGGAGC CAGCCCTGAG
    CTAGCCAAGA GCCCACGCCT GAGAAACGCG GCCGACCTGC CTCCACCGCT CCCCAACAAG
    CCTCCCCCTG AGGACTACTA TGAAGAGGCC CTTCCTCTGG GACCCGGCAA GTCGCCTGAG
    TACATCAGCT CCCACAATGG CTGCAGCCCC TCACACTCGA TTGTGGATGG CTACTATGAG
    GACGCAGACA GCAGCTACCC TGCAACCAGG GTGAACGGCG AGCTTAAGAG CTCCTATAAT
    GACTCTGACG CAATGAGCAG CTCCTATGAG TCCTACGATG AAGAGGAGGA GGAAGGGAAG
    AGCCCGCAGC CCCGACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAA
    TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGTTTCG GGCAGTGGGC CAAGCAGCTG
    ACGGTCATCA GGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGATCG GCAGCCACAT
    CTGAGGTTGG CACTGGATAC CTGCAGCATC ATCTACGTGC CCAAGGACAG CCGGCACAAG
    AGGCACGAGC TGCGTTTCAC CCAGGGGGCT ACCGAGGTCT TGGTGCTGGC ACTGCAGAGC
    CGAGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAAG TGAGCAAGCC AGTTGGGGGA
    GCTGAGGGAG TGGAGGTCCC CAGATCCCCA GTCCTCCTGT GCAAGTTGGA CCTGGACAAG
    AGGCTGTCCC AAGAGAAGCA GACCTCAGAT TCTGACAGCG TGGGTGTGGG TGACAACTGT
    TCTACCCTTG GCCGCCGGGA GACCTGTGAT CACGGCAAAG GGAAGAAGAG CAGCCTGGCA
    GAACTGAAGG GCTCAATGAG CAGGGCTGCG GGCCGCAAGA TCACCCGTAT CATTGGCTTC
    TCCAAGAAGA AGACACTGGC CGATGACCTG CAGACGTCCT CCACCGAGGA GGAGGTTCCC
    TGCTGTGGCT ACCTGAACGT GCTGGTGAAC CAGGGCTGGA AGGAACGCTG GTGCCGCCTG
    AAGTGCAACA CTCTGTATTT CCACAAGGAT CACATGGACC TGCGAACCCA TGTGAACGCC
    ATCGCCCTGC AAGGCTGTGA GGTGGCCCCG GGCTTTGGGC CCCGACACCC ATTTGCCTTC
    AGGATCCTGC GCAACCGGCA GGAGGTGGCC ATCTTGGAGG CAAGCTGTTC AGAGGACATG
    GGTCGCTGGC TCGGGCTGCT GCTGGTGGAG ATGGGCTCCA GAGTCACTCC GGAGGCGCTG
    CACTATGACT ACGTGGATGT GGAGACCTTA ACCAGCATCG TCAGTGCTGG GCGCAACTCC
    TTCCTATATG CAAGATCCTG CCAGAATCAG TGGCCTGAGC CCCGAGTCTA TGATGATGTT
    CCTTATGAAA AGATGCAGGA CGAGGAGCCC GAGCGCCCCA CAGGGGCCCA GGTGAAGCGT
    CACGCCTCCT CCTGCAGTGA GAAGTCCCAT CGTGTGGACC CGCAGGTCAA AGTCAAACGC
    CACGCCTCCA GTGCCAATCA ATACAAGTAT GGCAAGAACC GAGCCGAGGA GGATGCCCGG
    AGGTACTTGG TAGAAAAAGA GAAGCTGGAG AAAGAGAAAG AGACGATTCG GACAGAGCTG
    ATAGCACTGA GACAGGAGAA GAGGGAACTG AAGGAAGCCA TTCGGAGCAG CCCAGGAGCA
    AAATTAAAGG CTCTGGAAGA AGCCGTGGCC ACCCTGGAAG CTCAGTGTCG GGCAAAGGAG
    GAGCGCCGGA TTGACCTGGA GCTGAAGCTG GTGGCTGTGA AGGAGCGCTT GCAGCAGTCC
    CTGGCAGGAG GGCCAGCCCT GGGGCTCTCC GTGAGCAGCA AGCCCAAGAG TGGGGAATGG
    GAAATGAAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139809.1
    CDS111..2288
    Translation

    Target ORF information:

    RefSeq Version NM_001146337.3
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001146337.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGACCGAG GCCAGGTGCT GGAGCAGCTG CTCCCAGAGC TCACCGGGCT GCTCAGCCTC 
    CTGGACCACG AGTACCTCAG CGATACCACC CTGGAAAAGA AGATGGCCGT GGCCTCCATC
    CTGCAGAGCC TGCAGCCCCT TCCAGCAAAG GAGGTCTCCT ACCTGTATGT GAACACAGCA
    GACCTCCACT CGGGGCCCAG CTTCGTGGAA TCCCTCTTTG AAGAATTTGA CTGTGACCTG
    AGTGACCTTC GGGACATGCC AGAGGATGAT GGGGAGCCCA GCAAAGGAGC CAGCCCTGAG
    CTAGCCAAGA GCCCACGCCT GAGAAACGCG GCCGACCTGC CTCCACCGCT CCCCAACAAG
    CCTCCCCCTG AGGACTACTA TGAAGAGGCC CTTCCTCTGG GACCCGGCAA GTCGCCTGAG
    TACATCAGCT CCCACAATGG CTGCAGCCCC TCACACTCGA TTGTGGATGG CTACTATGAG
    GACGCAGACA GCAGCTACCC TGCAACCAGG GTGAACGGCG AGCTTAAGAG CTCCTATAAT
    GACTCTGACG CAATGAGCAG CTCCTATGAG TCCTACGATG AAGAGGAGGA GGAAGGGAAG
    AGCCCGCAGC CCCGACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAA
    TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGTTTCG GGCAGTGGGC CAAGCAGCTG
    ACGGTCATCA GGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGATCG GCAGCCACAT
    CTGAGGTTGG CACTGGATAC CTGCAGCATC ATCTACGTGC CCAAGGACAG CCGGCACAAG
    AGGCACGAGC TGCGTTTCAC CCAGGGGGCT ACCGAGGTCT TGGTGCTGGC ACTGCAGAGC
    CGAGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAAG TGAGCAAGCC AGTTGGGGGA
    GCTGAGGGAG TGGAGGTCCC CAGATCCCCA GTCCTCCTGT GCAAGTTGGA CCTGGACAAG
    AGGCTGTCCC AAGAGAAGCA GACCTCAGAT TCTGACAGCG TGGGTGTGGG TGACAACTGT
    TCTACCCTTG GCCGCCGGGA GACCTGTGAT CACGGCAAAG GGAAGAAGAG CAGCCTGGCA
    GAACTGAAGG GCTCAATGAG CAGGGCTGCG GGCCGCAAGA TCACCCGTAT CATTGGCTTC
    TCCAAGAAGA AGACACTGGC CGATGACCTG CAGACGTCCT CCACCGAGGA GGAGGTTCCC
    TGCTGTGGCT ACCTGAACGT GCTGGTGAAC CAGGGCTGGA AGGAACGCTG GTGCCGCCTG
    AAGTGCAACA CTCTGTATTT CCACAAGGAT CACATGGACC TGCGAACCCA TGTGAACGCC
    ATCGCCCTGC AAGGCTGTGA GGTGGCCCCG GGCTTTGGGC CCCGACACCC ATTTGCCTTC
    AGGATCCTGC GCAACCGGCA GGAGGTGGCC ATCTTGGAGG CAAGCTGTTC AGAGGACATG
    GGTCGCTGGC TCGGGCTGCT GCTGGTGGAG ATGGGCTCCA GAGTCACTCC GGAGGCGCTG
    CACTATGACT ACGTGGATGT GGAGACCTTA ACCAGCATCG TCAGTGCTGG GCGCAACTCC
    TTCCTATATG CAAGATCCTG CCAGAATCAG TGGCCTGAGC CCCGAGTCTA TGATGATGTT
    CCTTATGAAA AGATGCAGGA CGAGGAGCCC GAGCGCCCCA CAGGGGCCCA GGTGAAGCGT
    CACGCCTCCT CCTGCAGTGA GAAGTCCCAT CGTGTGGACC CGCAGGTCAA AGTCAAACGC
    CACGCCTCCA GTGCCAATCA ATACAAGTAT GGCAAGAACC GAGCCGAGGA GGATGCCCGG
    AGGTACTTGG TAGAAAAAGA GAAGCTGGAG AAAGAGAAAG AGACGATTCG GACAGAGCTG
    ATAGCACTGA GACAGGAGAA GAGGGAACTG AAGGAAGCCA TTCGGAGCAG CCCAGGAGCA
    AAATTAAAGG CTCTGGAAGA AGCCGTGGCC ACCCTGGAAG CTCAGTGTCG GGCAAAGGAG
    GAGCGCCGGA TTGACCTGGA GCTGAAGCTG GTGGCTGTGA AGGAGCGCTT GCAGCAGTCC
    CTGGCAGGAG GGCCAGCCCT GGGGCTCTCC GTGAGCAGCA AGCCCAAGAG TGGGGAATGG
    GAAATGAAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11195
    Clone ID Related Accession (Same CDS sequence) NM_001146337.3 , NM_001146337.2
    Accession Version NM_001146337.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1-like 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA792192.1, BC125093.1, BC125094.1, AK074185.1, AC131025.1 and BX107758.1. On Apr 16, 2016 this sequence version replaced NM_001146337.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC125094.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153031, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGACCGAG GCCAGGTGCT GGAGCAGCTG CTCCCAGAGC TCACCGGGCT GCTCAGCCTC 
    CTGGACCACG AGTACCTCAG CGATACCACC CTGGAAAAGA AGATGGCCGT GGCCTCCATC
    CTGCAGAGCC TGCAGCCCCT TCCAGCAAAG GAGGTCTCCT ACCTGTATGT GAACACAGCA
    GACCTCCACT CGGGGCCCAG CTTCGTGGAA TCCCTCTTTG AAGAATTTGA CTGTGACCTG
    AGTGACCTTC GGGACATGCC AGAGGATGAT GGGGAGCCCA GCAAAGGAGC CAGCCCTGAG
    CTAGCCAAGA GCCCACGCCT GAGAAACGCG GCCGACCTGC CTCCACCGCT CCCCAACAAG
    CCTCCCCCTG AGGACTACTA TGAAGAGGCC CTTCCTCTGG GACCCGGCAA GTCGCCTGAG
    TACATCAGCT CCCACAATGG CTGCAGCCCC TCACACTCGA TTGTGGATGG CTACTATGAG
    GACGCAGACA GCAGCTACCC TGCAACCAGG GTGAACGGCG AGCTTAAGAG CTCCTATAAT
    GACTCTGACG CAATGAGCAG CTCCTATGAG TCCTACGATG AAGAGGAGGA GGAAGGGAAG
    AGCCCGCAGC CCCGACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAA
    TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGTTTCG GGCAGTGGGC CAAGCAGCTG
    ACGGTCATCA GGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGATCG GCAGCCACAT
    CTGAGGTTGG CACTGGATAC CTGCAGCATC ATCTACGTGC CCAAGGACAG CCGGCACAAG
    AGGCACGAGC TGCGTTTCAC CCAGGGGGCT ACCGAGGTCT TGGTGCTGGC ACTGCAGAGC
    CGAGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAAG TGAGCAAGCC AGTTGGGGGA
    GCTGAGGGAG TGGAGGTCCC CAGATCCCCA GTCCTCCTGT GCAAGTTGGA CCTGGACAAG
    AGGCTGTCCC AAGAGAAGCA GACCTCAGAT TCTGACAGCG TGGGTGTGGG TGACAACTGT
    TCTACCCTTG GCCGCCGGGA GACCTGTGAT CACGGCAAAG GGAAGAAGAG CAGCCTGGCA
    GAACTGAAGG GCTCAATGAG CAGGGCTGCG GGCCGCAAGA TCACCCGTAT CATTGGCTTC
    TCCAAGAAGA AGACACTGGC CGATGACCTG CAGACGTCCT CCACCGAGGA GGAGGTTCCC
    TGCTGTGGCT ACCTGAACGT GCTGGTGAAC CAGGGCTGGA AGGAACGCTG GTGCCGCCTG
    AAGTGCAACA CTCTGTATTT CCACAAGGAT CACATGGACC TGCGAACCCA TGTGAACGCC
    ATCGCCCTGC AAGGCTGTGA GGTGGCCCCG GGCTTTGGGC CCCGACACCC ATTTGCCTTC
    AGGATCCTGC GCAACCGGCA GGAGGTGGCC ATCTTGGAGG CAAGCTGTTC AGAGGACATG
    GGTCGCTGGC TCGGGCTGCT GCTGGTGGAG ATGGGCTCCA GAGTCACTCC GGAGGCGCTG
    CACTATGACT ACGTGGATGT GGAGACCTTA ACCAGCATCG TCAGTGCTGG GCGCAACTCC
    TTCCTATATG CAAGATCCTG CCAGAATCAG TGGCCTGAGC CCCGAGTCTA TGATGATGTT
    CCTTATGAAA AGATGCAGGA CGAGGAGCCC GAGCGCCCCA CAGGGGCCCA GGTGAAGCGT
    CACGCCTCCT CCTGCAGTGA GAAGTCCCAT CGTGTGGACC CGCAGGTCAA AGTCAAACGC
    CACGCCTCCA GTGCCAATCA ATACAAGTAT GGCAAGAACC GAGCCGAGGA GGATGCCCGG
    AGGTACTTGG TAGAAAAAGA GAAGCTGGAG AAAGAGAAAG AGACGATTCG GACAGAGCTG
    ATAGCACTGA GACAGGAGAA GAGGGAACTG AAGGAAGCCA TTCGGAGCAG CCCAGGAGCA
    AAATTAAAGG CTCTGGAAGA AGCCGTGGCC ACCCTGGAAG CTCAGTGTCG GGCAAAGGAG
    GAGCGCCGGA TTGACCTGGA GCTGAAGCTG GTGGCTGTGA AGGAGCGCTT GCAGCAGTCC
    CTGGCAGGAG GGCCAGCCCT GGGGCTCTCC GTGAGCAGCA AGCCCAAGAG TGGGGAATGG
    GAAATGAAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139809.1
    CDS132..2309
    Translation

    Target ORF information:

    RefSeq Version NM_001146337.2
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001146337.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGACCGAG GCCAGGTGCT GGAGCAGCTG CTCCCAGAGC TCACCGGGCT GCTCAGCCTC 
    CTGGACCACG AGTACCTCAG CGATACCACC CTGGAAAAGA AGATGGCCGT GGCCTCCATC
    CTGCAGAGCC TGCAGCCCCT TCCAGCAAAG GAGGTCTCCT ACCTGTATGT GAACACAGCA
    GACCTCCACT CGGGGCCCAG CTTCGTGGAA TCCCTCTTTG AAGAATTTGA CTGTGACCTG
    AGTGACCTTC GGGACATGCC AGAGGATGAT GGGGAGCCCA GCAAAGGAGC CAGCCCTGAG
    CTAGCCAAGA GCCCACGCCT GAGAAACGCG GCCGACCTGC CTCCACCGCT CCCCAACAAG
    CCTCCCCCTG AGGACTACTA TGAAGAGGCC CTTCCTCTGG GACCCGGCAA GTCGCCTGAG
    TACATCAGCT CCCACAATGG CTGCAGCCCC TCACACTCGA TTGTGGATGG CTACTATGAG
    GACGCAGACA GCAGCTACCC TGCAACCAGG GTGAACGGCG AGCTTAAGAG CTCCTATAAT
    GACTCTGACG CAATGAGCAG CTCCTATGAG TCCTACGATG AAGAGGAGGA GGAAGGGAAG
    AGCCCGCAGC CCCGACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAA
    TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGTTTCG GGCAGTGGGC CAAGCAGCTG
    ACGGTCATCA GGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGATCG GCAGCCACAT
    CTGAGGTTGG CACTGGATAC CTGCAGCATC ATCTACGTGC CCAAGGACAG CCGGCACAAG
    AGGCACGAGC TGCGTTTCAC CCAGGGGGCT ACCGAGGTCT TGGTGCTGGC ACTGCAGAGC
    CGAGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAAG TGAGCAAGCC AGTTGGGGGA
    GCTGAGGGAG TGGAGGTCCC CAGATCCCCA GTCCTCCTGT GCAAGTTGGA CCTGGACAAG
    AGGCTGTCCC AAGAGAAGCA GACCTCAGAT TCTGACAGCG TGGGTGTGGG TGACAACTGT
    TCTACCCTTG GCCGCCGGGA GACCTGTGAT CACGGCAAAG GGAAGAAGAG CAGCCTGGCA
    GAACTGAAGG GCTCAATGAG CAGGGCTGCG GGCCGCAAGA TCACCCGTAT CATTGGCTTC
    TCCAAGAAGA AGACACTGGC CGATGACCTG CAGACGTCCT CCACCGAGGA GGAGGTTCCC
    TGCTGTGGCT ACCTGAACGT GCTGGTGAAC CAGGGCTGGA AGGAACGCTG GTGCCGCCTG
    AAGTGCAACA CTCTGTATTT CCACAAGGAT CACATGGACC TGCGAACCCA TGTGAACGCC
    ATCGCCCTGC AAGGCTGTGA GGTGGCCCCG GGCTTTGGGC CCCGACACCC ATTTGCCTTC
    AGGATCCTGC GCAACCGGCA GGAGGTGGCC ATCTTGGAGG CAAGCTGTTC AGAGGACATG
    GGTCGCTGGC TCGGGCTGCT GCTGGTGGAG ATGGGCTCCA GAGTCACTCC GGAGGCGCTG
    CACTATGACT ACGTGGATGT GGAGACCTTA ACCAGCATCG TCAGTGCTGG GCGCAACTCC
    TTCCTATATG CAAGATCCTG CCAGAATCAG TGGCCTGAGC CCCGAGTCTA TGATGATGTT
    CCTTATGAAA AGATGCAGGA CGAGGAGCCC GAGCGCCCCA CAGGGGCCCA GGTGAAGCGT
    CACGCCTCCT CCTGCAGTGA GAAGTCCCAT CGTGTGGACC CGCAGGTCAA AGTCAAACGC
    CACGCCTCCA GTGCCAATCA ATACAAGTAT GGCAAGAACC GAGCCGAGGA GGATGCCCGG
    AGGTACTTGG TAGAAAAAGA GAAGCTGGAG AAAGAGAAAG AGACGATTCG GACAGAGCTG
    ATAGCACTGA GACAGGAGAA GAGGGAACTG AAGGAAGCCA TTCGGAGCAG CCCAGGAGCA
    AAATTAAAGG CTCTGGAAGA AGCCGTGGCC ACCCTGGAAG CTCAGTGTCG GGCAAAGGAG
    GAGCGCCGGA TTGACCTGGA GCTGAAGCTG GTGGCTGTGA AGGAGCGCTT GCAGCAGTCC
    CTGGCAGGAG GGCCAGCCCT GGGGCTCTCC GTGAGCAGCA AGCCCAAGAG TGGGGAATGG
    GAAATGAAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39817
    Clone ID Related Accession (Same CDS sequence) NM_001323062.1 , NM_001323062.2
    Accession Version NM_001323062.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-04
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1-like 1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA792192.1, BC125093.1, AK074185.1, AC131025.1 and BX107758.1. On Apr 16, 2016 this sequence version replaced XM_005268373.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.98295.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGACCGAG GCCAGGTGCT GGAGCAGCTG CTCCCAGAGC TCACCGGGCT GCTCAGCCTC 
    CTGGACCACG AGTACCTCAG CGATACCACC CTGGAAAAGA AGATGGCCGT GGCCTCCATC
    CTGCAGAGCC TGCAGCCCCT TCCAGCAAAG GAGGTCTCCT ACCTGTATGT GAACACAGCA
    GACCTCCACT CGGGGCCCAG CTTCGTGGAA TCCCTCTTTG AAGAATTTGA CTGTGACCTG
    AGTGACCTTC GGGACATGCC AGAGGATGAT GGGGAGCCCA GCAAAGGAGC CAGCCCTGAG
    CTAGCCAAGA GCCCACGCCT GAGAAACGCG GCCGACCTGC CTCCACCGCT CCCCAACAAG
    CCTCCCCCTG AGGACTACTA TGAAGAGGCC CTTCCTCTGG GACCCGGCAA GTCGCCTGAG
    TACATCAGCT CCCACAATAA TGACTCTGAC GCAATGAGCA GCTCCTATGA GTCCTACGAT
    GAAGAGGAGG AGGAAGGGAA GAGCCCGCAG CCCCGACACC AGTGGCCCTC AGAGGAGGCC
    TCCATGCACC TGGTGAGGGA ATGCAGGATA TGTGCCTTCC TGCTGCGGAA AAAGCGTTTC
    GGGCAGTGGG CCAAGCAGCT GACGGTCATC AGGGAGGACC AGCTCCTGTG TTACAAAAGC
    TCCAAGGATC GGCAGCCACA TCTGAGGTTG GCACTGGATA CCTGCAGCAT CATCTACGTG
    CCCAAGGACA GCCGGCACAA GAGGCACGAG CTGCGTTTCA CCCAGGGGGC TACCGAGGTC
    TTGGTGCTGG CACTGCAGAG CCGAGAGCAG GCCGAGGAGT GGCTGAAGGT CATCCGAGAA
    GTGAGCAAGC CAGTTGGGGG AGCTGAGGGA GTGGAGGTCC CCAGATCCCC AGTCCTCCTG
    TGCAAGTTGG ACCTGGACAA GAGGCTGTCC CAAGAGAAGC AGACCTCAGA TTCTGACAGC
    GTGGGTGTGG GTGACAACTG TTCTACCCTT GGCCGCCGGG AGACCTGTGA TCACGGCAAA
    GGGAAGAAGA GCAGCCTGGC AGAACTGAAG GGCTCAATGA GCAGGGCTGC GGGCCGCAAG
    ATCACCCGTA TCATTGGCTT CTCCAAGAAG AAGACACTGG CCGATGACCT GCAGACGTCC
    TCCACCGAGG AGGAGGTTCC CTGCTGTGGC TACCTGAACG TGCTGGTGAA CCAGGGCTGG
    AAGGAACGCT GGTGCCGCCT GAAGTGCAAC ACTCTGTATT TCCACAAGGA TCACATGGAC
    CTGCGAACCC ATGTGAACGC CATCGCCCTG CAAGGCTGTG AGGTGGCCCC GGGCTTTGGG
    CCCCGACACC CATTTGCCTT CAGGATCCTG CGCAACCGGC AGGAGGTGGC CATCTTGGAG
    GCAAGCTGTT CAGAGGACAT GGGTCGCTGG CTCGGGCTGC TGCTGGTGGA GATGGGCTCC
    AGAGTCACTC CGGAGGCGCT GCACTATGAC TACGTGGATG TGGAGACCTT AACCAGCATC
    GTCAGTGCTG GGCGCAACTC CTTCCTATAT GCAAGATCCT GCCAGAATCA GTGGCCTGAG
    CCCCGAGTCT ATGATGATGT TCCTTATGAA AAGATGCAGG ACGAGGAGCC CGAGCGCCCC
    ACAGGGGCCC AGGTGAAGCG TCACGCCTCC TCCTGCAGTG AGAAGTCCCA TCGTGTGGAC
    CCGCAGGTCA AAGTCAAACG CCACGCCTCC AGTGCCAATC AATACAAGTA TGGCAAGAAC
    CGAGCCGAGG AGGATGCCCG GAGGTACTTG GTAGAAAAAG AGAAGCTGGA GAAAGAGAAA
    GAGACGATTC GGACAGAGCT GATAGCACTG AGACAGGAGA AGAGGGAACT GAAGGAAGCC
    ATTCGGAGCA GCCCAGGAGC AAAATTAAAG GCTCTGGAAG AAGCCGTGGC CACCCTGGAA
    GCTCAGTGTC GGGCAAAGGA GGAGCGCCGG ATTGACCTGG AGCTGAAGCT GGTGGCTGTG
    AAGGAGCGCT TGCAGCAGTC CCTGGCAGGA GGGCCAGCCC TGGGGCTCTC CGTGAGCAGC
    AAGCCCAAGA GTGGGGAAAC TGCAAATAAA CCCCAGAACA GCGTTCCAGA GCAACCTCTC
    CCTGTCAACT GTGTTTCTGA GCTGAGGAAG AGGAGCCCAT CCATCGTAGC CTCCAACCAA
    GGAAGGGTGC TACAGAAAGC CAAGGAATGG GAAATGAAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001309991.1
    CDS132..2339
    Translation

    Target ORF information:

    RefSeq Version NM_001323062.1
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001323062.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGACCGAG GCCAGGTGCT GGAGCAGCTG CTCCCAGAGC TCACCGGGCT GCTCAGCCTC 
    CTGGACCACG AGTACCTCAG CGATACCACC CTGGAAAAGA AGATGGCCGT GGCCTCCATC
    CTGCAGAGCC TGCAGCCCCT TCCAGCAAAG GAGGTCTCCT ACCTGTATGT GAACACAGCA
    GACCTCCACT CGGGGCCCAG CTTCGTGGAA TCCCTCTTTG AAGAATTTGA CTGTGACCTG
    AGTGACCTTC GGGACATGCC AGAGGATGAT GGGGAGCCCA GCAAAGGAGC CAGCCCTGAG
    CTAGCCAAGA GCCCACGCCT GAGAAACGCG GCCGACCTGC CTCCACCGCT CCCCAACAAG
    CCTCCCCCTG AGGACTACTA TGAAGAGGCC CTTCCTCTGG GACCCGGCAA GTCGCCTGAG
    TACATCAGCT CCCACAATAA TGACTCTGAC GCAATGAGCA GCTCCTATGA GTCCTACGAT
    GAAGAGGAGG AGGAAGGGAA GAGCCCGCAG CCCCGACACC AGTGGCCCTC AGAGGAGGCC
    TCCATGCACC TGGTGAGGGA ATGCAGGATA TGTGCCTTCC TGCTGCGGAA AAAGCGTTTC
    GGGCAGTGGG CCAAGCAGCT GACGGTCATC AGGGAGGACC AGCTCCTGTG TTACAAAAGC
    TCCAAGGATC GGCAGCCACA TCTGAGGTTG GCACTGGATA CCTGCAGCAT CATCTACGTG
    CCCAAGGACA GCCGGCACAA GAGGCACGAG CTGCGTTTCA CCCAGGGGGC TACCGAGGTC
    TTGGTGCTGG CACTGCAGAG CCGAGAGCAG GCCGAGGAGT GGCTGAAGGT CATCCGAGAA
    GTGAGCAAGC CAGTTGGGGG AGCTGAGGGA GTGGAGGTCC CCAGATCCCC AGTCCTCCTG
    TGCAAGTTGG ACCTGGACAA GAGGCTGTCC CAAGAGAAGC AGACCTCAGA TTCTGACAGC
    GTGGGTGTGG GTGACAACTG TTCTACCCTT GGCCGCCGGG AGACCTGTGA TCACGGCAAA
    GGGAAGAAGA GCAGCCTGGC AGAACTGAAG GGCTCAATGA GCAGGGCTGC GGGCCGCAAG
    ATCACCCGTA TCATTGGCTT CTCCAAGAAG AAGACACTGG CCGATGACCT GCAGACGTCC
    TCCACCGAGG AGGAGGTTCC CTGCTGTGGC TACCTGAACG TGCTGGTGAA CCAGGGCTGG
    AAGGAACGCT GGTGCCGCCT GAAGTGCAAC ACTCTGTATT TCCACAAGGA TCACATGGAC
    CTGCGAACCC ATGTGAACGC CATCGCCCTG CAAGGCTGTG AGGTGGCCCC GGGCTTTGGG
    CCCCGACACC CATTTGCCTT CAGGATCCTG CGCAACCGGC AGGAGGTGGC CATCTTGGAG
    GCAAGCTGTT CAGAGGACAT GGGTCGCTGG CTCGGGCTGC TGCTGGTGGA GATGGGCTCC
    AGAGTCACTC CGGAGGCGCT GCACTATGAC TACGTGGATG TGGAGACCTT AACCAGCATC
    GTCAGTGCTG GGCGCAACTC CTTCCTATAT GCAAGATCCT GCCAGAATCA GTGGCCTGAG
    CCCCGAGTCT ATGATGATGT TCCTTATGAA AAGATGCAGG ACGAGGAGCC CGAGCGCCCC
    ACAGGGGCCC AGGTGAAGCG TCACGCCTCC TCCTGCAGTG AGAAGTCCCA TCGTGTGGAC
    CCGCAGGTCA AAGTCAAACG CCACGCCTCC AGTGCCAATC AATACAAGTA TGGCAAGAAC
    CGAGCCGAGG AGGATGCCCG GAGGTACTTG GTAGAAAAAG AGAAGCTGGA GAAAGAGAAA
    GAGACGATTC GGACAGAGCT GATAGCACTG AGACAGGAGA AGAGGGAACT GAAGGAAGCC
    ATTCGGAGCA GCCCAGGAGC AAAATTAAAG GCTCTGGAAG AAGCCGTGGC CACCCTGGAA
    GCTCAGTGTC GGGCAAAGGA GGAGCGCCGG ATTGACCTGG AGCTGAAGCT GGTGGCTGTG
    AAGGAGCGCT TGCAGCAGTC CCTGGCAGGA GGGCCAGCCC TGGGGCTCTC CGTGAGCAGC
    AAGCCCAAGA GTGGGGAAAC TGCAAATAAA CCCCAGAACA GCGTTCCAGA GCAACCTCTC
    CCTGTCAACT GTGTTTCTGA GCTGAGGAAG AGGAGCCCAT CCATCGTAGC CTCCAACCAA
    GGAAGGGTGC TACAGAAAGC CAAGGAATGG GAAATGAAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39817
    Clone ID Related Accession (Same CDS sequence) NM_001323062.1 , NM_001323062.2
    Accession Version NM_001323062.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-08
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1-like 1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA792192.1, BC125093.1, AK074185.1, AC131025.1 and BX107758.1. On May 31, 2019 this sequence version replaced NM_001323062.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.98295.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGACCGAG GCCAGGTGCT GGAGCAGCTG CTCCCAGAGC TCACCGGGCT GCTCAGCCTC 
    CTGGACCACG AGTACCTCAG CGATACCACC CTGGAAAAGA AGATGGCCGT GGCCTCCATC
    CTGCAGAGCC TGCAGCCCCT TCCAGCAAAG GAGGTCTCCT ACCTGTATGT GAACACAGCA
    GACCTCCACT CGGGGCCCAG CTTCGTGGAA TCCCTCTTTG AAGAATTTGA CTGTGACCTG
    AGTGACCTTC GGGACATGCC AGAGGATGAT GGGGAGCCCA GCAAAGGAGC CAGCCCTGAG
    CTAGCCAAGA GCCCACGCCT GAGAAACGCG GCCGACCTGC CTCCACCGCT CCCCAACAAG
    CCTCCCCCTG AGGACTACTA TGAAGAGGCC CTTCCTCTGG GACCCGGCAA GTCGCCTGAG
    TACATCAGCT CCCACAATAA TGACTCTGAC GCAATGAGCA GCTCCTATGA GTCCTACGAT
    GAAGAGGAGG AGGAAGGGAA GAGCCCGCAG CCCCGACACC AGTGGCCCTC AGAGGAGGCC
    TCCATGCACC TGGTGAGGGA ATGCAGGATA TGTGCCTTCC TGCTGCGGAA AAAGCGTTTC
    GGGCAGTGGG CCAAGCAGCT GACGGTCATC AGGGAGGACC AGCTCCTGTG TTACAAAAGC
    TCCAAGGATC GGCAGCCACA TCTGAGGTTG GCACTGGATA CCTGCAGCAT CATCTACGTG
    CCCAAGGACA GCCGGCACAA GAGGCACGAG CTGCGTTTCA CCCAGGGGGC TACCGAGGTC
    TTGGTGCTGG CACTGCAGAG CCGAGAGCAG GCCGAGGAGT GGCTGAAGGT CATCCGAGAA
    GTGAGCAAGC CAGTTGGGGG AGCTGAGGGA GTGGAGGTCC CCAGATCCCC AGTCCTCCTG
    TGCAAGTTGG ACCTGGACAA GAGGCTGTCC CAAGAGAAGC AGACCTCAGA TTCTGACAGC
    GTGGGTGTGG GTGACAACTG TTCTACCCTT GGCCGCCGGG AGACCTGTGA TCACGGCAAA
    GGGAAGAAGA GCAGCCTGGC AGAACTGAAG GGCTCAATGA GCAGGGCTGC GGGCCGCAAG
    ATCACCCGTA TCATTGGCTT CTCCAAGAAG AAGACACTGG CCGATGACCT GCAGACGTCC
    TCCACCGAGG AGGAGGTTCC CTGCTGTGGC TACCTGAACG TGCTGGTGAA CCAGGGCTGG
    AAGGAACGCT GGTGCCGCCT GAAGTGCAAC ACTCTGTATT TCCACAAGGA TCACATGGAC
    CTGCGAACCC ATGTGAACGC CATCGCCCTG CAAGGCTGTG AGGTGGCCCC GGGCTTTGGG
    CCCCGACACC CATTTGCCTT CAGGATCCTG CGCAACCGGC AGGAGGTGGC CATCTTGGAG
    GCAAGCTGTT CAGAGGACAT GGGTCGCTGG CTCGGGCTGC TGCTGGTGGA GATGGGCTCC
    AGAGTCACTC CGGAGGCGCT GCACTATGAC TACGTGGATG TGGAGACCTT AACCAGCATC
    GTCAGTGCTG GGCGCAACTC CTTCCTATAT GCAAGATCCT GCCAGAATCA GTGGCCTGAG
    CCCCGAGTCT ATGATGATGT TCCTTATGAA AAGATGCAGG ACGAGGAGCC CGAGCGCCCC
    ACAGGGGCCC AGGTGAAGCG TCACGCCTCC TCCTGCAGTG AGAAGTCCCA TCGTGTGGAC
    CCGCAGGTCA AAGTCAAACG CCACGCCTCC AGTGCCAATC AATACAAGTA TGGCAAGAAC
    CGAGCCGAGG AGGATGCCCG GAGGTACTTG GTAGAAAAAG AGAAGCTGGA GAAAGAGAAA
    GAGACGATTC GGACAGAGCT GATAGCACTG AGACAGGAGA AGAGGGAACT GAAGGAAGCC
    ATTCGGAGCA GCCCAGGAGC AAAATTAAAG GCTCTGGAAG AAGCCGTGGC CACCCTGGAA
    GCTCAGTGTC GGGCAAAGGA GGAGCGCCGG ATTGACCTGG AGCTGAAGCT GGTGGCTGTG
    AAGGAGCGCT TGCAGCAGTC CCTGGCAGGA GGGCCAGCCC TGGGGCTCTC CGTGAGCAGC
    AAGCCCAAGA GTGGGGAAAC TGCAAATAAA CCCCAGAACA GCGTTCCAGA GCAACCTCTC
    CCTGTCAACT GTGTTTCTGA GCTGAGGAAG AGGAGCCCAT CCATCGTAGC CTCCAACCAA
    GGAAGGGTGC TACAGAAAGC CAAGGAATGG GAAATGAAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001309991.1
    CDS111..2318
    Translation

    Target ORF information:

    RefSeq Version NM_001323062.2
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001323062.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGACCGAG GCCAGGTGCT GGAGCAGCTG CTCCCAGAGC TCACCGGGCT GCTCAGCCTC 
    CTGGACCACG AGTACCTCAG CGATACCACC CTGGAAAAGA AGATGGCCGT GGCCTCCATC
    CTGCAGAGCC TGCAGCCCCT TCCAGCAAAG GAGGTCTCCT ACCTGTATGT GAACACAGCA
    GACCTCCACT CGGGGCCCAG CTTCGTGGAA TCCCTCTTTG AAGAATTTGA CTGTGACCTG
    AGTGACCTTC GGGACATGCC AGAGGATGAT GGGGAGCCCA GCAAAGGAGC CAGCCCTGAG
    CTAGCCAAGA GCCCACGCCT GAGAAACGCG GCCGACCTGC CTCCACCGCT CCCCAACAAG
    CCTCCCCCTG AGGACTACTA TGAAGAGGCC CTTCCTCTGG GACCCGGCAA GTCGCCTGAG
    TACATCAGCT CCCACAATAA TGACTCTGAC GCAATGAGCA GCTCCTATGA GTCCTACGAT
    GAAGAGGAGG AGGAAGGGAA GAGCCCGCAG CCCCGACACC AGTGGCCCTC AGAGGAGGCC
    TCCATGCACC TGGTGAGGGA ATGCAGGATA TGTGCCTTCC TGCTGCGGAA AAAGCGTTTC
    GGGCAGTGGG CCAAGCAGCT GACGGTCATC AGGGAGGACC AGCTCCTGTG TTACAAAAGC
    TCCAAGGATC GGCAGCCACA TCTGAGGTTG GCACTGGATA CCTGCAGCAT CATCTACGTG
    CCCAAGGACA GCCGGCACAA GAGGCACGAG CTGCGTTTCA CCCAGGGGGC TACCGAGGTC
    TTGGTGCTGG CACTGCAGAG CCGAGAGCAG GCCGAGGAGT GGCTGAAGGT CATCCGAGAA
    GTGAGCAAGC CAGTTGGGGG AGCTGAGGGA GTGGAGGTCC CCAGATCCCC AGTCCTCCTG
    TGCAAGTTGG ACCTGGACAA GAGGCTGTCC CAAGAGAAGC AGACCTCAGA TTCTGACAGC
    GTGGGTGTGG GTGACAACTG TTCTACCCTT GGCCGCCGGG AGACCTGTGA TCACGGCAAA
    GGGAAGAAGA GCAGCCTGGC AGAACTGAAG GGCTCAATGA GCAGGGCTGC GGGCCGCAAG
    ATCACCCGTA TCATTGGCTT CTCCAAGAAG AAGACACTGG CCGATGACCT GCAGACGTCC
    TCCACCGAGG AGGAGGTTCC CTGCTGTGGC TACCTGAACG TGCTGGTGAA CCAGGGCTGG
    AAGGAACGCT GGTGCCGCCT GAAGTGCAAC ACTCTGTATT TCCACAAGGA TCACATGGAC
    CTGCGAACCC ATGTGAACGC CATCGCCCTG CAAGGCTGTG AGGTGGCCCC GGGCTTTGGG
    CCCCGACACC CATTTGCCTT CAGGATCCTG CGCAACCGGC AGGAGGTGGC CATCTTGGAG
    GCAAGCTGTT CAGAGGACAT GGGTCGCTGG CTCGGGCTGC TGCTGGTGGA GATGGGCTCC
    AGAGTCACTC CGGAGGCGCT GCACTATGAC TACGTGGATG TGGAGACCTT AACCAGCATC
    GTCAGTGCTG GGCGCAACTC CTTCCTATAT GCAAGATCCT GCCAGAATCA GTGGCCTGAG
    CCCCGAGTCT ATGATGATGT TCCTTATGAA AAGATGCAGG ACGAGGAGCC CGAGCGCCCC
    ACAGGGGCCC AGGTGAAGCG TCACGCCTCC TCCTGCAGTG AGAAGTCCCA TCGTGTGGAC
    CCGCAGGTCA AAGTCAAACG CCACGCCTCC AGTGCCAATC AATACAAGTA TGGCAAGAAC
    CGAGCCGAGG AGGATGCCCG GAGGTACTTG GTAGAAAAAG AGAAGCTGGA GAAAGAGAAA
    GAGACGATTC GGACAGAGCT GATAGCACTG AGACAGGAGA AGAGGGAACT GAAGGAAGCC
    ATTCGGAGCA GCCCAGGAGC AAAATTAAAG GCTCTGGAAG AAGCCGTGGC CACCCTGGAA
    GCTCAGTGTC GGGCAAAGGA GGAGCGCCGG ATTGACCTGG AGCTGAAGCT GGTGGCTGTG
    AAGGAGCGCT TGCAGCAGTC CCTGGCAGGA GGGCCAGCCC TGGGGCTCTC CGTGAGCAGC
    AAGCCCAAGA GTGGGGAAAC TGCAAATAAA CCCCAGAACA GCGTTCCAGA GCAACCTCTC
    CCTGTCAACT GTGTTTCTGA GCTGAGGAAG AGGAGCCCAT CCATCGTAGC CTCCAACCAA
    GGAAGGGTGC TACAGAAAGC CAAGGAATGG GAAATGAAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.