AFAP1L1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	AFAP1L1
Entrez Gene ID	510738
Full Name	actin filament associated protein 1 like 1
General protein information	Preferred Names actin filament associated protein 1 like 1 Names actin filament-associated protein 1-like 1 AFAP1-like protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024994080.1	XP_024849848.1	actin filament-associated protein 1-like 1 isoform X1
NM_001100334.2	NP_001093804.1	actin filament-associated protein 1-like 1
XM_024994081.1	XP_024849849.1	actin filament-associated protein 1-like 1 isoform X2
XM_024994083.1	XP_024849851.1	actin filament-associated protein 1-like 1 isoform X3

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	afap1l1	XP_002939152.2
Bos taurus (cattle)	AFAP1L1	NP_001093804.1
Gallus gallus (chicken)	AFAP1L1	XP_425193.3
Homo sapiens (human)	AFAP1L1	NP_689619.1
Canis lupus familiaris (dog)	AFAP1L1	XP_005619510.1
Pan troglodytes (chimpanzee)	AFAP1L1	XP_518027.3
	AFAP1L1	XP_002804621.1
Mus musculus (house mouse)	Afap1l1	NP_849259.2
Rattus norvegicus (Norway rat)	Afap1l1	NP_001099612.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AFAP1L1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFAP1L1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa235940	XM_024994080.1 Latest version!	Bos taurus actin filament associated protein 1 like 1 (AFAP1L1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa235941	XM_024994081.1 Latest version!	Bos taurus actin filament associated protein 1 like 1 (AFAP1L1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa235942	XM_024994083.1 Latest version!	Bos taurus actin filament associated protein 1 like 1 (AFAP1L1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa44714	NM_001100334.2 Latest version!	Bos taurus actin filament associated protein 1 like 1 (AFAP1L1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFAP1L1 ( XM_024994080.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849848.1 Bos taurus actin filament associated protein 1 like 1 (AFAP1L1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa235940
Clone ID Related Accession (Same CDS sequence)	XM_024994080.1
Accession Version	XM_024994080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	actin filament-associated protein 1-like 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGAG GCCGGGTGCT GGAGCAGCTC CTCCCTGAGC TCACAGGGCT GCTCAGCCTC 
CTGGACCACG AGTACCTCAG TGACACCACT CTGGAGAAGA AGATGGCCGT GGCCTCCATC 
CTGCAGAGCC TGCAGCCCCT CCCAGCCAAA GAAGTCTCCT ACCTGTATGT GAACACAGCG 
GACCTCCACT CTGGGCCCAG CTTCGTGGAG TCCCTCTTCG AAGAATTTGA CTGTGACCTG 
AGCGGCCTTC AAGATATGCC AGAGGATGAG GCGGAGTCCT GCAAAGCAGC CAGTCCTGAG 
CCAGCCAAGA GTCCATCTCT GAGACACACA GCTGACCTGC CTCCACCACT CCCCAACAGG 
CCTCCGCCTG AGGACTACTA TGAGGAGGCC CTTCCCCTGG GACCTGGCAA GTCCCCTGAG 
TACATCAGCT CCCACAGAGG ACACCGTGTC CAAGATCTTT GCCAGCCCTG GCACCTGGAG 
TCTGCCTGCA TTTCTCCAAG TACTGCTCTT TCTACCAACC CCACAGATGG CTGCAGCCCA 
GCCCACTCGC TTATGGACGG CTACTATGAG GATGCAGACA GCAGCTACCC CGCCACCAGG 
ATGAACGGGG AGCTGAAGAA TTCTTACAAT GACTCTGACG CCATGAGCAG CTCCTACGAG 
TCGTACGATG AGGAGGAGGA GGAAGGGAAA GGGCCACAGC CCACACACCA GTGGCCCTCA 
GAGGAGGCCT CCATGCACCT GGTGAGGGAC TGCAGGATAT GTGCCTTCCT GCTGCGGAAA 
AAGCGCTTCG GGCAGTGGGC CAAGCAGTTG ACTGTCATCA AGGAGGACCA GCTCCTGTGT 
TACAAAAGCT CCAAGGACCG GCAGCCGCAC CTGAGGCTAG CGCTGGATGT CTGCAGTGTC 
ATCTATGTGC CCAAGGACAG CCGGCACAAG AGGCACGAGC TGCGCTTCGC CCAGGGGGCT 
ACTGAAGTCC TGGTGCTGGC CCTGCAGAGC CGGGAGCAGG CCGAGGAGTG GCTGAAGGTC 
ATCCGAGAGG TCAGCAAACC CGTCGGGGGC ACTGAGGGGG CAGACGTGCC CAGATCCCCA 
GTCCTCCTGT GCAAGGCGGA CCTGGACAAG AGGCTGTCCC AAGAGAAGCA GACCTCGGAT 
TCTGACAGCC TAGGCATGGG TGACAGCTGT TCCACTCTGG GCCGTGAACA TGGCAAAGGG 
AAGAAGAGCA GCCTGTCAGA GCTGAAGGGT TCAATGAGCA GAGCTGCAGG CCGCAAGATC 
ACCCGTATCA TCAGCTTCTC TAAGAAGAAG GCACTGGCTG ATGACCTGCA GGCGTCCTCT 
ACTGAGGAGG TTCCGTGCTG TGGTTATCTG AATGTGCTGG TGAACCACGG ATGGAAGGAA 
CGCTGGTGCC GCCTGAAGTG CAACACCTTG TATTTCCATA AGGACCGCAC AGACCTGCGG 
ACCCACGTGA ACGCCATTGC CCTCCGCGGT TGTGAGGTGG CCCCAGGCTT TGGGCCCAGA 
CACCCATTTG CCTTCAGGAT CCTACACAAC CGGCAGGAGG TCGCTATCTT AGAGGCAAGC 
TGCTCAGAGG ACATGGGCCG CTGGCTTGGG CTGCTGCTAG TGGAGATGGG CTCCAAAGTC 
ACTCCCGAAG CATTGCACTA TGACTATGTG GATGTGGAGA CCCTAACCAG CATCGTCAGT 
GCAGGGCGCA ACTCCTTCTT ATATGCAAGA TCCTGCCAGG ATCAGTGGCC TGAGCCCCGA 
GTCTATGATG ATGTTCCTTA TGAAAAGATG CAGGACGAGG AGCCTGAGCG TCCCCCTGGA 
GCCCAGGTCA AGCGCCACGC CTCCACCTGC AGTGAGAAGT CCCATCGGGT GGACCCACAG 
GTCAAAGTCA AGCGCCACGC CTCCAGTGCC CATCAGTATA AGTACGGTAA GAACCGAGCT 
GAGGAGGATG CCAGGAGGTA CCTGGTAGAA AAAGAGAAGC TGGAGAAAGA GAAAGAGACA 
ATCCGGACAG AGCTGATGGC GCTGCGGCAG GAAAAGAGGG AACTAAAGGA AGCCATTCGG 
AACAACCCAG GGGCAAAGTT AAAGGCTCTT GAAGAGGCTC TGGCCACCCT CGAAGCTCAG 
TGTCGGGCAA AGGAGGAGCA CCGGATCGAC CTGGAGCTGC GGCTGGTGAC TGTGAAGGAG 
CGCTTGCAGC AGTCCCTGGC AGGGGGCCCA GCCCTGGGGC TCTCCGTCAA CAGCAAGATC 
AAGAGTGGGG AAACTGCAAA TAAACCCCAG AACAATGTCC CAGAGCAACC TCTCCCTGTC 
AATTGTGTTT CCGAGTTACG GAAGAGGAGC CCATCCATCA TAAACTCCAA TCAAGGAAGG 
GTGCTACAAA AAGCCAAGGA ATGGGAAATG AAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849848.1
CDS	109..2490
Translation	MDRGRVLEQLLPELTGLLSLLDHEYLSDTTLEKKMAVASILQSLQPLPAKEVSYLYVNTADLHSGPSFVESLFEEFDCDLSGLQDMPEDEAESCKAASPEPAKSPSLRHTADLPPPLPNRPPPEDYYEEALPLGPGKSPEYISSHRGHRVQDLCQPWHLESACISPSTALSTNPTDGCSPAHSLMDGYYEDADSSYPATRMNGELKNSYNDSDAMSSSYESYDEEEEEGKGPQPTHQWPSEEASMHLVRDCRICAFLLRKKRFGQWAKQLTVIKEDQLLCYKSSKDRQPHLRLALDVCSVIYVPKDSRHKRHELRFAQGATEVLVLALQSREQAEEWLKVIREVSKPVGGTEGADVPRSPVLLCKADLDKRLSQEKQTSDSDSLGMGDSCSTLGREHGKGKKSSLSELKGSMSRAAGRKITRIISFSKKKALADDLQASSTEEVPCCGYLNVLVNHGWKERWCRLKCNTLYFHKDRTDLRTHVNAIALRGCEVAPGFGPRHPFAFRILHNRQEVAILEASCSEDMGRWLGLLLVEMGSKVTPEALHYDYVDVETLTSIVSAGRNSFLYARSCQDQWPEPRVYDDVPYEKMQDEEPERPPGAQVKRHASTCSEKSHRVDPQVKVKRHASSAHQYKYGKNRAEEDARRYLVEKEKLEKEKETIRTELMALRQEKRELKEAIRNNPGAKLKALEEALATLEAQCRAKEEHRIDLELRLVTVKERLQQSLAGGPALGLSVNSKIKSGETANKPQNNVPEQPLPVNCVSELRKRSPSIINSNQGRVLQKAKEWEMKKT

Target ORF information:

RefSeq Version	XM_024994080.1
Organism	Bos taurus(cattle)
Definition	Bos taurus actin filament associated protein 1 like 1 (AFAP1L1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994080.1

ORF Insert Sequence:

ATGGACCGAG GCCGGGTGCT GGAGCAGCTC CTCCCTGAGC TCACAGGGCT GCTCAGCCTC 
CTGGACCACG AGTACCTCAG TGACACCACT CTGGAGAAGA AGATGGCCGT GGCCTCCATC 
CTGCAGAGCC TGCAGCCCCT CCCAGCCAAA GAAGTCTCCT ACCTGTATGT GAACACAGCG 
GACCTCCACT CTGGGCCCAG CTTCGTGGAG TCCCTCTTCG AAGAATTTGA CTGTGACCTG 
AGCGGCCTTC AAGATATGCC AGAGGATGAG GCGGAGTCCT GCAAAGCAGC CAGTCCTGAG 
CCAGCCAAGA GTCCATCTCT GAGACACACA GCTGACCTGC CTCCACCACT CCCCAACAGG 
CCTCCGCCTG AGGACTACTA TGAGGAGGCC CTTCCCCTGG GACCTGGCAA GTCCCCTGAG 
TACATCAGCT CCCACAGAGG ACACCGTGTC CAAGATCTTT GCCAGCCCTG GCACCTGGAG 
TCTGCCTGCA TTTCTCCAAG TACTGCTCTT TCTACCAACC CCACAGATGG CTGCAGCCCA 
GCCCACTCGC TTATGGACGG CTACTATGAG GATGCAGACA GCAGCTACCC CGCCACCAGG 
ATGAACGGGG AGCTGAAGAA TTCTTACAAT GACTCTGACG CCATGAGCAG CTCCTACGAG 
TCGTACGATG AGGAGGAGGA GGAAGGGAAA GGGCCACAGC CCACACACCA GTGGCCCTCA 
GAGGAGGCCT CCATGCACCT GGTGAGGGAC TGCAGGATAT GTGCCTTCCT GCTGCGGAAA 
AAGCGCTTCG GGCAGTGGGC CAAGCAGTTG ACTGTCATCA AGGAGGACCA GCTCCTGTGT 
TACAAAAGCT CCAAGGACCG GCAGCCGCAC CTGAGGCTAG CGCTGGATGT CTGCAGTGTC 
ATCTATGTGC CCAAGGACAG CCGGCACAAG AGGCACGAGC TGCGCTTCGC CCAGGGGGCT 
ACTGAAGTCC TGGTGCTGGC CCTGCAGAGC CGGGAGCAGG CCGAGGAGTG GCTGAAGGTC 
ATCCGAGAGG TCAGCAAACC CGTCGGGGGC ACTGAGGGGG CAGACGTGCC CAGATCCCCA 
GTCCTCCTGT GCAAGGCGGA CCTGGACAAG AGGCTGTCCC AAGAGAAGCA GACCTCGGAT 
TCTGACAGCC TAGGCATGGG TGACAGCTGT TCCACTCTGG GCCGTGAACA TGGCAAAGGG 
AAGAAGAGCA GCCTGTCAGA GCTGAAGGGT TCAATGAGCA GAGCTGCAGG CCGCAAGATC 
ACCCGTATCA TCAGCTTCTC TAAGAAGAAG GCACTGGCTG ATGACCTGCA GGCGTCCTCT 
ACTGAGGAGG TTCCGTGCTG TGGTTATCTG AATGTGCTGG TGAACCACGG ATGGAAGGAA 
CGCTGGTGCC GCCTGAAGTG CAACACCTTG TATTTCCATA AGGACCGCAC AGACCTGCGG 
ACCCACGTGA ACGCCATTGC CCTCCGCGGT TGTGAGGTGG CCCCAGGCTT TGGGCCCAGA 
CACCCATTTG CCTTCAGGAT CCTACACAAC CGGCAGGAGG TCGCTATCTT AGAGGCAAGC 
TGCTCAGAGG ACATGGGCCG CTGGCTTGGG CTGCTGCTAG TGGAGATGGG CTCCAAAGTC 
ACTCCCGAAG CATTGCACTA TGACTATGTG GATGTGGAGA CCCTAACCAG CATCGTCAGT 
GCAGGGCGCA ACTCCTTCTT ATATGCAAGA TCCTGCCAGG ATCAGTGGCC TGAGCCCCGA 
GTCTATGATG ATGTTCCTTA TGAAAAGATG CAGGACGAGG AGCCTGAGCG TCCCCCTGGA 
GCCCAGGTCA AGCGCCACGC CTCCACCTGC AGTGAGAAGT CCCATCGGGT GGACCCACAG 
GTCAAAGTCA AGCGCCACGC CTCCAGTGCC CATCAGTATA AGTACGGTAA GAACCGAGCT 
GAGGAGGATG CCAGGAGGTA CCTGGTAGAA AAAGAGAAGC TGGAGAAAGA GAAAGAGACA 
ATCCGGACAG AGCTGATGGC GCTGCGGCAG GAAAAGAGGG AACTAAAGGA AGCCATTCGG 
AACAACCCAG GGGCAAAGTT AAAGGCTCTT GAAGAGGCTC TGGCCACCCT CGAAGCTCAG 
TGTCGGGCAA AGGAGGAGCA CCGGATCGAC CTGGAGCTGC GGCTGGTGAC TGTGAAGGAG 
CGCTTGCAGC AGTCCCTGGC AGGGGGCCCA GCCCTGGGGC TCTCCGTCAA CAGCAAGATC 
AAGAGTGGGG AAACTGCAAA TAAACCCCAG AACAATGTCC CAGAGCAACC TCTCCCTGTC 
AATTGTGTTT CCGAGTTACG GAAGAGGAGC CCATCCATCA TAAACTCCAA TCAAGGAAGG 
GTGCTACAAA AAGCCAAGGA ATGGGAAATG AAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFAP1L1 ( XM_024994081.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849849.1 Bos taurus actin filament associated protein 1 like 1 (AFAP1L1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa235941
Clone ID Related Accession (Same CDS sequence)	XM_024994081.1
Accession Version	XM_024994081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	actin filament-associated protein 1-like 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGAG GCCGGGTGCT GGAGCAGCTC CTCCCTGAGC TCACAGGGCT GCTCAGCCTC 
CTGGACCACG AGTACCTCAG TGACACCACT CTGGAGAAGA AGATGGCCGT GGCCTCCATC 
CTGCAGAGCC TGCAGCCCCT CCCAGCCAAA GAAGTCTCCT ACCTGTATGT GAACACAGCG 
GACCTCCACT CTGGGCCCAG CTTCGTGGAG TCCCTCTTCG AAGAATTTGA CTGTGACCTG 
AGCGGCCTTC AAGATATGCC AGAGGATGAG GCGGAGTCCT GCAAAGCAGC CAGTCCTGAG 
CCAGCCAAGA GTCCATCTCT GAGACACACA GCTGACCTGC CTCCACCACT CCCCAACAGG 
CCTCCGCCTG AGGACTACTA TGAGGAGGCC CTTCCCCTGG GACCTGGCAA GTCCCCTGAG 
TACATCAGCT CCCACAATGG CTGCAGCCCA GCCCACTCGC TTATGGACGG CTACTATGAG 
GATGCAGACA GCAGCTACCC CGCCACCAGG ATGAACGGGG AGCTGAAGAA TTCTTACAAT 
GACTCTGACG CCATGAGCAG CTCCTACGAG TCGTACGATG AGGAGGAGGA GGAAGGGAAA 
GGGCCACAGC CCACACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAC 
TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGCTTCG GGCAGTGGGC CAAGCAGTTG 
ACTGTCATCA AGGAGGACCA GCTCCTGGAG GCCCTCACCC CTTCTCTTCC TGTCCAGTGT 
TACAAAAGCT CCAAGGACCG GCAGCCGCAC CTGAGGCTAG CGCTGGATGT CTGCAGTGTC 
ATCTATGTGC CCAAGGACAG CCGGCACAAG AGGCACGAGC TGCGCTTCGC CCAGGGGGCT 
ACTGAAGTCC TGGTGCTGGC CCTGCAGAGC CGGGAGCAGG CCGAGGAGTG GCTGAAGGTC 
ATCCGAGAGG TCAGCAAACC CGTCGGGGGC ACTGAGGGGG CAGACGTGCC CAGATCCCCA 
GTCCTCCTGT GCAAGGCGGA CCTGGACAAG AGGCTGTCCC AAGAGAAGCA GACCTCGGAT 
TCTGACAGCC TAGGCATGGG TGACAGCTGT TCCACTCTGG GCCGTGAACA TGGCAAAGGG 
AAGAAGAGCA GCCTGTCAGA GCTGAAGGGT TCAATGAGCA GAGCTGCAGG CCGCAAGATC 
ACCCGTATCA TCAGCTTCTC TAAGAAGAAG GCACTGGCTG ATGACCTGCA GGCGTCCTCT 
ACTGAGGAGG TTCCGTGCTG TGGTTATCTG AATGTGCTGG TGAACCACGG ATGGAAGGAA 
CGCTGGTGCC GCCTGAAGTG CAACACCTTG TATTTCCATA AGGACCGCAC AGACCTGCGG 
ACCCACGTGA ACGCCATTGC CCTCCGCGGT TGTGAGGTGG CCCCAGGCTT TGGGCCCAGA 
CACCCATTTG CCTTCAGGAT CCTACACAAC CGGCAGGAGG TCGCTATCTT AGAGGCAAGC 
TGCTCAGAGG ACATGGGCCG CTGGCTTGGG CTGCTGCTAG TGGAGATGGG CTCCAAAGTC 
ACTCCCGAAG CATTGCACTA TGACTATGTG GATGTGGAGA CCCTAACCAG CATCGTCAGT 
GCAGGGCGCA ACTCCTTCTT ATATGCAAGA TCCTGCCAGG ATCAGTGGCC TGAGCCCCGA 
GTCTATGATG ATGTTCCTTA TGAAAAGATG CAGGACGAGG AGCCTGAGCG TCCCCCTGGA 
GCCCAGGTCA AGCGCCACGC CTCCACCTGC AGTGAGAAGT CCCATCGGGT GGACCCACAG 
GTCAAAGTCA AGCGCCACGC CTCCAGTGCC CATCAGTATA AGTACGGTAA GAACCGAGCT 
GAGGAGGATG CCAGGAGGTA CCTGGTAGAA AAAGAGAAGC TGGAGAAAGA GAAAGAGACA 
ATCCGGACAG AGCTGATGGC GCTGCGGCAG GAAAAGAGGG AACTAAAGGA AGCCATTCGG 
AACAACCCAG GGGCAAAGTT AAAGGCTCTT GAAGAGGCTC TGGCCACCCT CGAAGCTCAG 
TGTCGGGCAA AGGAGGAGCA CCGGATCGAC CTGGAGCTGC GGCTGGTGAC TGTGAAGGAG 
CGCTTGCAGC AGTCCCTGGC AGGGGGCCCA GCCCTGGGGC TCTCCGTCAA CAGCAAGATC 
AAGAGTGGGG AAACTGCAAA TAAACCCCAG AACAATGTCC CAGAGCAACC TCTCCCTGTC 
AATTGTGTTT CCGAGTTACG GAAGAGGAGC CCATCCATCA TAAACTCCAA TCAAGGAAGG 
GTGCTACAAA AAGCCAAGGA ATGGGAAATG AAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849849.1
CDS	109..2430
Translation	MDRGRVLEQLLPELTGLLSLLDHEYLSDTTLEKKMAVASILQSLQPLPAKEVSYLYVNTADLHSGPSFVESLFEEFDCDLSGLQDMPEDEAESCKAASPEPAKSPSLRHTADLPPPLPNRPPPEDYYEEALPLGPGKSPEYISSHNGCSPAHSLMDGYYEDADSSYPATRMNGELKNSYNDSDAMSSSYESYDEEEEEGKGPQPTHQWPSEEASMHLVRDCRICAFLLRKKRFGQWAKQLTVIKEDQLLEALTPSLPVQCYKSSKDRQPHLRLALDVCSVIYVPKDSRHKRHELRFAQGATEVLVLALQSREQAEEWLKVIREVSKPVGGTEGADVPRSPVLLCKADLDKRLSQEKQTSDSDSLGMGDSCSTLGREHGKGKKSSLSELKGSMSRAAGRKITRIISFSKKKALADDLQASSTEEVPCCGYLNVLVNHGWKERWCRLKCNTLYFHKDRTDLRTHVNAIALRGCEVAPGFGPRHPFAFRILHNRQEVAILEASCSEDMGRWLGLLLVEMGSKVTPEALHYDYVDVETLTSIVSAGRNSFLYARSCQDQWPEPRVYDDVPYEKMQDEEPERPPGAQVKRHASTCSEKSHRVDPQVKVKRHASSAHQYKYGKNRAEEDARRYLVEKEKLEKEKETIRTELMALRQEKRELKEAIRNNPGAKLKALEEALATLEAQCRAKEEHRIDLELRLVTVKERLQQSLAGGPALGLSVNSKIKSGETANKPQNNVPEQPLPVNCVSELRKRSPSIINSNQGRVLQKAKEWEMKKT

Target ORF information:

RefSeq Version	XM_024994081.1
Organism	Bos taurus(cattle)
Definition	Bos taurus actin filament associated protein 1 like 1 (AFAP1L1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994081.1

ORF Insert Sequence:

ATGGACCGAG GCCGGGTGCT GGAGCAGCTC CTCCCTGAGC TCACAGGGCT GCTCAGCCTC 
CTGGACCACG AGTACCTCAG TGACACCACT CTGGAGAAGA AGATGGCCGT GGCCTCCATC 
CTGCAGAGCC TGCAGCCCCT CCCAGCCAAA GAAGTCTCCT ACCTGTATGT GAACACAGCG 
GACCTCCACT CTGGGCCCAG CTTCGTGGAG TCCCTCTTCG AAGAATTTGA CTGTGACCTG 
AGCGGCCTTC AAGATATGCC AGAGGATGAG GCGGAGTCCT GCAAAGCAGC CAGTCCTGAG 
CCAGCCAAGA GTCCATCTCT GAGACACACA GCTGACCTGC CTCCACCACT CCCCAACAGG 
CCTCCGCCTG AGGACTACTA TGAGGAGGCC CTTCCCCTGG GACCTGGCAA GTCCCCTGAG 
TACATCAGCT CCCACAATGG CTGCAGCCCA GCCCACTCGC TTATGGACGG CTACTATGAG 
GATGCAGACA GCAGCTACCC CGCCACCAGG ATGAACGGGG AGCTGAAGAA TTCTTACAAT 
GACTCTGACG CCATGAGCAG CTCCTACGAG TCGTACGATG AGGAGGAGGA GGAAGGGAAA 
GGGCCACAGC CCACACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAC 
TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGCTTCG GGCAGTGGGC CAAGCAGTTG 
ACTGTCATCA AGGAGGACCA GCTCCTGGAG GCCCTCACCC CTTCTCTTCC TGTCCAGTGT 
TACAAAAGCT CCAAGGACCG GCAGCCGCAC CTGAGGCTAG CGCTGGATGT CTGCAGTGTC 
ATCTATGTGC CCAAGGACAG CCGGCACAAG AGGCACGAGC TGCGCTTCGC CCAGGGGGCT 
ACTGAAGTCC TGGTGCTGGC CCTGCAGAGC CGGGAGCAGG CCGAGGAGTG GCTGAAGGTC 
ATCCGAGAGG TCAGCAAACC CGTCGGGGGC ACTGAGGGGG CAGACGTGCC CAGATCCCCA 
GTCCTCCTGT GCAAGGCGGA CCTGGACAAG AGGCTGTCCC AAGAGAAGCA GACCTCGGAT 
TCTGACAGCC TAGGCATGGG TGACAGCTGT TCCACTCTGG GCCGTGAACA TGGCAAAGGG 
AAGAAGAGCA GCCTGTCAGA GCTGAAGGGT TCAATGAGCA GAGCTGCAGG CCGCAAGATC 
ACCCGTATCA TCAGCTTCTC TAAGAAGAAG GCACTGGCTG ATGACCTGCA GGCGTCCTCT 
ACTGAGGAGG TTCCGTGCTG TGGTTATCTG AATGTGCTGG TGAACCACGG ATGGAAGGAA 
CGCTGGTGCC GCCTGAAGTG CAACACCTTG TATTTCCATA AGGACCGCAC AGACCTGCGG 
ACCCACGTGA ACGCCATTGC CCTCCGCGGT TGTGAGGTGG CCCCAGGCTT TGGGCCCAGA 
CACCCATTTG CCTTCAGGAT CCTACACAAC CGGCAGGAGG TCGCTATCTT AGAGGCAAGC 
TGCTCAGAGG ACATGGGCCG CTGGCTTGGG CTGCTGCTAG TGGAGATGGG CTCCAAAGTC 
ACTCCCGAAG CATTGCACTA TGACTATGTG GATGTGGAGA CCCTAACCAG CATCGTCAGT 
GCAGGGCGCA ACTCCTTCTT ATATGCAAGA TCCTGCCAGG ATCAGTGGCC TGAGCCCCGA 
GTCTATGATG ATGTTCCTTA TGAAAAGATG CAGGACGAGG AGCCTGAGCG TCCCCCTGGA 
GCCCAGGTCA AGCGCCACGC CTCCACCTGC AGTGAGAAGT CCCATCGGGT GGACCCACAG 
GTCAAAGTCA AGCGCCACGC CTCCAGTGCC CATCAGTATA AGTACGGTAA GAACCGAGCT 
GAGGAGGATG CCAGGAGGTA CCTGGTAGAA AAAGAGAAGC TGGAGAAAGA GAAAGAGACA 
ATCCGGACAG AGCTGATGGC GCTGCGGCAG GAAAAGAGGG AACTAAAGGA AGCCATTCGG 
AACAACCCAG GGGCAAAGTT AAAGGCTCTT GAAGAGGCTC TGGCCACCCT CGAAGCTCAG 
TGTCGGGCAA AGGAGGAGCA CCGGATCGAC CTGGAGCTGC GGCTGGTGAC TGTGAAGGAG 
CGCTTGCAGC AGTCCCTGGC AGGGGGCCCA GCCCTGGGGC TCTCCGTCAA CAGCAAGATC 
AAGAGTGGGG AAACTGCAAA TAAACCCCAG AACAATGTCC CAGAGCAACC TCTCCCTGTC 
AATTGTGTTT CCGAGTTACG GAAGAGGAGC CCATCCATCA TAAACTCCAA TCAAGGAAGG 
GTGCTACAAA AAGCCAAGGA ATGGGAAATG AAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFAP1L1 ( XM_024994083.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849851.1 Bos taurus actin filament associated protein 1 like 1 (AFAP1L1), transcript variant X3, mRNA.

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CloneID	OBa235942
Clone ID Related Accession (Same CDS sequence)	XM_024994083.1
Accession Version	XM_024994083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	actin filament-associated protein 1-like 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGAG GCCGGGTGCT GGAGCAGCTC CTCCCTGAGC TCACAGGGCT GCTCAGCCTC 
CTGGACCACG AGTACCTCAG TGACACCACT CTGGAGAAGA AGATGGCCGT GGCCTCCATC 
CTGCAGAGCC TGCAGCCCCT CCCAGCCAAA GAAGTCTCCT ACCTGTATGT GAACACAGCG 
GACCTCCACT CTGGGCCCAG CTTCGTGGAG TCCCTCTTCG AAGAATTTGA CTGTGACCTG 
AGCGGCCTTC AAGATATGCC AGAGGATGAG GCGGAGTCCT GCAAAGCAGC CAGTCCTGAG 
CCAGCCAAGA GTCCATCTCT GAGACACACA GCTGACCTGC CTCCACCACT CCCCAACAGG 
CCTCCGCCTG AGGACTACTA TGAGGAGGCC CTTCCCCTGG GACCTGGCAA GTCCCCTGAG 
TACATCAGCT CCCACAGAGG ACACCGTGTC CAAGATCTTT GCCAGCCCTG GCACCTGGAG 
TCTGCCTGCA TTTCTCCAAG TACTGCTCTT TCTACCAACC CCACAGATGG CTGCAGCCCA 
GCCCACTCGC TTATGGACGG CTACTATGAG GATGCAGACA GCAGCTACCC CGCCACCAGG 
ATGAACGGGG AGCTGAAGAA TTCTTACAAT GACTCTGACG CCATGAGCAG CTCCTACGAG 
TCGTACGATG AGGAGGAGGA GGAAGGGAAA GGGCCACAGC CCACACACCA GTGGCCCTCA 
GAGGAGGCCT CCATGCACCT GGTGAGGGAC TGCAGGATAT GTGCCTTCCT GCTGCGGAAA 
AAGCGCTTCG GGCAGTGGGC CAAGCAGTTG ACTGTCATCA AGGAGGACCA GCTCCTGGTC 
ATCCGAGAGG TCAGCAAACC CGTCGGGGGC ACTGAGGGGG CAGACGTGCC CAGATCCCCA 
GTCCTCCTGT GCAAGGCGGA CCTGGACAAG AGGCTGTCCC AAGAGAAGCA GACCTCGGAT 
TCTGACAGCC TAGGCATGGG TGACAGCTGT TCCACTCTGG GCCGTGAACA TGGCAAAGGG 
AAGAAGAGCA GCCTGTCAGA GCTGAAGGGT TCAATGAGCA GAGCTGCAGG CCGCAAGATC 
ACCCGTATCA TCAGCTTCTC TAAGAAGAAG GCACTGGCTG ATGACCTGCA GGCGTCCTCT 
ACTGAGGAGG TTCCGTGCTG TGGTTATCTG AATGTGCTGG TGAACCACGG ATGGAAGGAA 
CGCTGGTGCC GCCTGAAGTG CAACACCTTG TATTTCCATA AGGACCGCAC AGACCTGCGG 
ACCCACGTGA ACGCCATTGC CCTCCGCGGT TGTGAGGTGG CCCCAGGCTT TGGGCCCAGA 
CACCCATTTG CCTTCAGGAT CCTACACAAC CGGCAGGAGG TCGCTATCTT AGAGGCAAGC 
TGCTCAGAGG ACATGGGCCG CTGGCTTGGG CTGCTGCTAG TGGAGATGGG CTCCAAAGTC 
ACTCCCGAAG CATTGCACTA TGACTATGTG GATGTGGAGA CCCTAACCAG CATCGTCAGT 
GCAGGGCGCA ACTCCTTCTT ATATGCAAGA TCCTGCCAGG ATCAGTGGCC TGAGCCCCGA 
GTCTATGATG ATGTTCCTTA TGAAAAGATG CAGGACGAGG AGCCTGAGCG TCCCCCTGGA 
GCCCAGGTCA AGCGCCACGC CTCCACCTGC AGTGAGAAGT CCCATCGGGT GGACCCACAG 
GTCAAAGTCA AGCGCCACGC CTCCAGTGCC CATCAGTATA AGTACGGTAA GAACCGAGCT 
GAGGAGGATG CCAGGAGGTA CCTGGTAGAA AAAGAGAAGC TGGAGAAAGA GAAAGAGACA 
ATCCGGACAG AGCTGATGGC GCTGCGGCAG GAAAAGAGGG AACTAAAGGA AGCCATTCGG 
AACAACCCAG GGGCAAAGTT AAAGGCTCTT GAAGAGGCTC TGGCCACCCT CGAAGCTCAG 
TGTCGGGCAA AGGAGGAGCA CCGGATCGAC CTGGAGCTGC GGCTGGTGAC TGTGAAGGAG 
CGCTTGCAGC AGTCCCTGGC AGGGGGCCCA GCCCTGGGGC TCTCCGTCAA CAGCAAGATC 
AAGAGTGGGG AAACTGCAAA TAAACCCCAG AACAATGTCC CAGAGCAACC TCTCCCTGTC 
AATTGTGTTT CCGAGTTACG GAAGAGGAGC CCATCCATCA TAAACTCCAA TCAAGGAAGG 
GTGCTACAAA AAGCCAAGGA ATGGGAAATG AAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849851.1
CDS	109..2310
Translation	MDRGRVLEQLLPELTGLLSLLDHEYLSDTTLEKKMAVASILQSLQPLPAKEVSYLYVNTADLHSGPSFVESLFEEFDCDLSGLQDMPEDEAESCKAASPEPAKSPSLRHTADLPPPLPNRPPPEDYYEEALPLGPGKSPEYISSHRGHRVQDLCQPWHLESACISPSTALSTNPTDGCSPAHSLMDGYYEDADSSYPATRMNGELKNSYNDSDAMSSSYESYDEEEEEGKGPQPTHQWPSEEASMHLVRDCRICAFLLRKKRFGQWAKQLTVIKEDQLLVIREVSKPVGGTEGADVPRSPVLLCKADLDKRLSQEKQTSDSDSLGMGDSCSTLGREHGKGKKSSLSELKGSMSRAAGRKITRIISFSKKKALADDLQASSTEEVPCCGYLNVLVNHGWKERWCRLKCNTLYFHKDRTDLRTHVNAIALRGCEVAPGFGPRHPFAFRILHNRQEVAILEASCSEDMGRWLGLLLVEMGSKVTPEALHYDYVDVETLTSIVSAGRNSFLYARSCQDQWPEPRVYDDVPYEKMQDEEPERPPGAQVKRHASTCSEKSHRVDPQVKVKRHASSAHQYKYGKNRAEEDARRYLVEKEKLEKEKETIRTELMALRQEKRELKEAIRNNPGAKLKALEEALATLEAQCRAKEEHRIDLELRLVTVKERLQQSLAGGPALGLSVNSKIKSGETANKPQNNVPEQPLPVNCVSELRKRSPSIINSNQGRVLQKAKEWEMKKT

Target ORF information:

RefSeq Version	XM_024994083.1
Organism	Bos taurus(cattle)
Definition	Bos taurus actin filament associated protein 1 like 1 (AFAP1L1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994083.1

ORF Insert Sequence:

ATGGACCGAG GCCGGGTGCT GGAGCAGCTC CTCCCTGAGC TCACAGGGCT GCTCAGCCTC 
CTGGACCACG AGTACCTCAG TGACACCACT CTGGAGAAGA AGATGGCCGT GGCCTCCATC 
CTGCAGAGCC TGCAGCCCCT CCCAGCCAAA GAAGTCTCCT ACCTGTATGT GAACACAGCG 
GACCTCCACT CTGGGCCCAG CTTCGTGGAG TCCCTCTTCG AAGAATTTGA CTGTGACCTG 
AGCGGCCTTC AAGATATGCC AGAGGATGAG GCGGAGTCCT GCAAAGCAGC CAGTCCTGAG 
CCAGCCAAGA GTCCATCTCT GAGACACACA GCTGACCTGC CTCCACCACT CCCCAACAGG 
CCTCCGCCTG AGGACTACTA TGAGGAGGCC CTTCCCCTGG GACCTGGCAA GTCCCCTGAG 
TACATCAGCT CCCACAGAGG ACACCGTGTC CAAGATCTTT GCCAGCCCTG GCACCTGGAG 
TCTGCCTGCA TTTCTCCAAG TACTGCTCTT TCTACCAACC CCACAGATGG CTGCAGCCCA 
GCCCACTCGC TTATGGACGG CTACTATGAG GATGCAGACA GCAGCTACCC CGCCACCAGG 
ATGAACGGGG AGCTGAAGAA TTCTTACAAT GACTCTGACG CCATGAGCAG CTCCTACGAG 
TCGTACGATG AGGAGGAGGA GGAAGGGAAA GGGCCACAGC CCACACACCA GTGGCCCTCA 
GAGGAGGCCT CCATGCACCT GGTGAGGGAC TGCAGGATAT GTGCCTTCCT GCTGCGGAAA 
AAGCGCTTCG GGCAGTGGGC CAAGCAGTTG ACTGTCATCA AGGAGGACCA GCTCCTGGTC 
ATCCGAGAGG TCAGCAAACC CGTCGGGGGC ACTGAGGGGG CAGACGTGCC CAGATCCCCA 
GTCCTCCTGT GCAAGGCGGA CCTGGACAAG AGGCTGTCCC AAGAGAAGCA GACCTCGGAT 
TCTGACAGCC TAGGCATGGG TGACAGCTGT TCCACTCTGG GCCGTGAACA TGGCAAAGGG 
AAGAAGAGCA GCCTGTCAGA GCTGAAGGGT TCAATGAGCA GAGCTGCAGG CCGCAAGATC 
ACCCGTATCA TCAGCTTCTC TAAGAAGAAG GCACTGGCTG ATGACCTGCA GGCGTCCTCT 
ACTGAGGAGG TTCCGTGCTG TGGTTATCTG AATGTGCTGG TGAACCACGG ATGGAAGGAA 
CGCTGGTGCC GCCTGAAGTG CAACACCTTG TATTTCCATA AGGACCGCAC AGACCTGCGG 
ACCCACGTGA ACGCCATTGC CCTCCGCGGT TGTGAGGTGG CCCCAGGCTT TGGGCCCAGA 
CACCCATTTG CCTTCAGGAT CCTACACAAC CGGCAGGAGG TCGCTATCTT AGAGGCAAGC 
TGCTCAGAGG ACATGGGCCG CTGGCTTGGG CTGCTGCTAG TGGAGATGGG CTCCAAAGTC 
ACTCCCGAAG CATTGCACTA TGACTATGTG GATGTGGAGA CCCTAACCAG CATCGTCAGT 
GCAGGGCGCA ACTCCTTCTT ATATGCAAGA TCCTGCCAGG ATCAGTGGCC TGAGCCCCGA 
GTCTATGATG ATGTTCCTTA TGAAAAGATG CAGGACGAGG AGCCTGAGCG TCCCCCTGGA 
GCCCAGGTCA AGCGCCACGC CTCCACCTGC AGTGAGAAGT CCCATCGGGT GGACCCACAG 
GTCAAAGTCA AGCGCCACGC CTCCAGTGCC CATCAGTATA AGTACGGTAA GAACCGAGCT 
GAGGAGGATG CCAGGAGGTA CCTGGTAGAA AAAGAGAAGC TGGAGAAAGA GAAAGAGACA 
ATCCGGACAG AGCTGATGGC GCTGCGGCAG GAAAAGAGGG AACTAAAGGA AGCCATTCGG 
AACAACCCAG GGGCAAAGTT AAAGGCTCTT GAAGAGGCTC TGGCCACCCT CGAAGCTCAG 
TGTCGGGCAA AGGAGGAGCA CCGGATCGAC CTGGAGCTGC GGCTGGTGAC TGTGAAGGAG 
CGCTTGCAGC AGTCCCTGGC AGGGGGCCCA GCCCTGGGGC TCTCCGTCAA CAGCAAGATC 
AAGAGTGGGG AAACTGCAAA TAAACCCCAG AACAATGTCC CAGAGCAACC TCTCCCTGTC 
AATTGTGTTT CCGAGTTACG GAAGAGGAGC CCATCCATCA TAAACTCCAA TCAAGGAAGG 
GTGCTACAAA AAGCCAAGGA ATGGGAAATG AAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFAP1L1 ( NM_001100334.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001093804.1 Bos taurus actin filament associated protein 1 like 1 (AFAP1L1), mRNA.

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CloneID	OBa44714
Clone ID Related Accession (Same CDS sequence)	NM_001100334.2
Accession Version	NM_001100334.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Bos taurus(cattle)
Product	actin filament-associated protein 1-like 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC150012.1. On Aug 30, 2012 this sequence version replaced NM_001100334.1. ##Evidence-Data-START## Transcript exon combination :: BC150012.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGACCGAG GCCGGGTGCT GGAGCAGCTC CTCCCTGAGC TCACAGGGCT GCTCAGCCTC 
CTGGACCACG AGTACCTCAG TGACACCACT CTGGAGAAGA AGATGGCCGT GGCCTCCATC 
CTGCAGAGCC TGCAGCCCCT CCCAGCCAAA GAAGTCTCCT ACCTGTATGT GAACACAGCG 
GACCTCCACT CTGGGCCCAG CTTCGTGGAG TCCCTCTTCG AAGAATTTGA CTGTGACCTG 
AGCGGCCTTC AAGATATGCC AGAGGATGAG GCGGAGTCCT GCAAAGCAGC CAGTCCTGAG 
CCAGCCAAGA GTCCATCTCT GAGACACACA GCTGACCTGC CTCCACCACT CCCCAACAGG 
CCTCCGCCTG AGGACTACTA TGAGGAGGCC CTTCCCCTGG GACCTGGCAA GTCCCCTGAG 
TACATCAGCT CCCACAATGG CTGCAGCCCA GCCCACTCGC TTATGGACGG CTACTATGAG 
GATGCAGACA GCAGCTACCC CGCCACCAGG ATGAACGGGG AGCTGAAGAA TTCTTACAAT 
GACTCTGACG CCATGAGCAG CTCCTACGAG TCGTACGATG AGGAGGAGGA GGAAGGGAAA 
GGGCCACAGC CCACACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAC 
TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGCTTCG GGCAGTGGGC CAAGCAGTTG 
ACTGTCATCA AGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGACCG GCAGCCGCAC 
CTGAGGCTAG CGCTGGATGT CTGCAGTGTC ATCTATGTGC CCAAGGACAG CCGGCACAAG 
AGGCACGAGC TGCGCTTCGC CCAGGGGGCT ACTGAAGTCC TGGTGCTGGC CCTGCAGAGC 
CGGGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAGG TCAGCAAACC CGTCGGGGGC 
ACTGAGGGGG CAGACGTGCC CAGATCCCCA GTCCTCCTGT GCAAGGCGGA CCTGGACAAG 
AGGCTGTCCC AAGAGAAGCA GACCTCGGAT TCTGACAGCC TAGGCATGGG TGACAGCTGT 
TCCACTCTGG GCCGTGAACA TGGCAAAGGG AAGAAGAGCA GCCTGTCAGA GCTGAAGGGT 
TCAATGAGCA GAGCTGCAGG CCGCAAGATC ACCCGTATCA TCAGCTTCTC TAAGAAGAAG 
GCACTGGCTG ATGACCTGCA GGCGTCCTCT ACTGAGGAGG TTCCGTGCTG TGGTTATCTG 
AATGTGCTGG TGAACCACGG ATGGAAGGAA CGCTGGTGCC GCCTGAAGTG CAACACCTTG 
TATTTCCATA AGGACCGCAC AGACCTGCGG ACCCACGTGA ACGCCATTGC CCTCCGCGGT 
TGTGAGGTGG CCCCAGGCTT TGGGCCCAGA CACCCATTTG CCTTCAGGAT CCTACACAAC 
CGGCAGGAGG TCGCTATCTT AGAGGCAAGC TGCTCAGAGG ACATGGGCCG CTGGCTTGGG 
CTGCTGCTAG TGGAGATGGG CTCCAAAGTC ACTCCCGAAG CATTGCACTA TGACTATGTG 
GATGTGGAGA CCCTAACCAG CATCGTCAGT GCAGGGCGCA ACTCCTTCTT ATATGCAAGA 
TCCTGCCAGG ATCAGTGGCC TGAGCCCCGA GTCTATGATG ATGTTCCTTA TGAAAAGATG 
CAGGACGAGG AGCCTGAGCG TCCCCCTGGA GCCCAGGTCA AGCGCCACGC CTCCACCTGC 
AGTGAGAAGT CCCATCGGGT GGACCCACAG GTCAAAGTCA AGCGCCACGC CTCCAGTGCC 
CATCAGTATA AGTACGGTAA GAACCGAGCT GAGGAGGATG CCAGGAGGTA CCTGGTAGAA 
AAAGAGAAGC TGGAGAAAGA GAAAGAGACA ATCCGGACAG AGCTGATGGC GCTGCGGCAG 
GAAAAGAGGG AACTAAAGGA AGCCATTCGG AACAACCCAG GGGCAAAGTT AAAGGCTCTT 
GAAGAGGCTC TGGCCACCCT CGAAGCTCAG TGTCGGGCAA AGGAGGAGCA CCGGATCGAC 
CTGGAGCTGC GGCTGGTGAC TGTGAAGGAG CGCTTGCAGC AGTCCCTGGC AGGGGGCCCA 
GCCCTGGGGC TCTCCGTCAA CAGCAAGATC AAGAGTGGGG AAACTGCAAA TAAACCCCAG 
AACAATGTCC CAGAGCAACC TCTCCCTGTC AATTGTGTTT CCGAGTTACG GAAGAGGAGC 
CCATCCATCA TAAACTCCAA TCAAGGAAGG GTGCTACAAA AAGCCAAGGA ATGGGAAATG 
AAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093804.1
CDS	46..2337
Translation	MDRGRVLEQLLPELTGLLSLLDHEYLSDTTLEKKMAVASILQSLQPLPAKEVSYLYVNTADLHSGPSFVESLFEEFDCDLSGLQDMPEDEAESCKAASPEPAKSPSLRHTADLPPPLPNRPPPEDYYEEALPLGPGKSPEYISSHNGCSPAHSLMDGYYEDADSSYPATRMNGELKNSYNDSDAMSSSYESYDEEEEEGKGPQPTHQWPSEEASMHLVRDCRICAFLLRKKRFGQWAKQLTVIKEDQLLCYKSSKDRQPHLRLALDVCSVIYVPKDSRHKRHELRFAQGATEVLVLALQSREQAEEWLKVIREVSKPVGGTEGADVPRSPVLLCKADLDKRLSQEKQTSDSDSLGMGDSCSTLGREHGKGKKSSLSELKGSMSRAAGRKITRIISFSKKKALADDLQASSTEEVPCCGYLNVLVNHGWKERWCRLKCNTLYFHKDRTDLRTHVNAIALRGCEVAPGFGPRHPFAFRILHNRQEVAILEASCSEDMGRWLGLLLVEMGSKVTPEALHYDYVDVETLTSIVSAGRNSFLYARSCQDQWPEPRVYDDVPYEKMQDEEPERPPGAQVKRHASTCSEKSHRVDPQVKVKRHASSAHQYKYGKNRAEEDARRYLVEKEKLEKEKETIRTELMALRQEKRELKEAIRNNPGAKLKALEEALATLEAQCRAKEEHRIDLELRLVTVKERLQQSLAGGPALGLSVNSKIKSGETANKPQNNVPEQPLPVNCVSELRKRSPSIINSNQGRVLQKAKEWEMKKT

Target ORF information:

RefSeq Version	NM_001100334.2
Organism	Bos taurus(cattle)
Definition	Bos taurus actin filament associated protein 1 like 1 (AFAP1L1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100334.2

ORF Insert Sequence:

ATGGACCGAG GCCGGGTGCT GGAGCAGCTC CTCCCTGAGC TCACAGGGCT GCTCAGCCTC 
CTGGACCACG AGTACCTCAG TGACACCACT CTGGAGAAGA AGATGGCCGT GGCCTCCATC 
CTGCAGAGCC TGCAGCCCCT CCCAGCCAAA GAAGTCTCCT ACCTGTATGT GAACACAGCG 
GACCTCCACT CTGGGCCCAG CTTCGTGGAG TCCCTCTTCG AAGAATTTGA CTGTGACCTG 
AGCGGCCTTC AAGATATGCC AGAGGATGAG GCGGAGTCCT GCAAAGCAGC CAGTCCTGAG 
CCAGCCAAGA GTCCATCTCT GAGACACACA GCTGACCTGC CTCCACCACT CCCCAACAGG 
CCTCCGCCTG AGGACTACTA TGAGGAGGCC CTTCCCCTGG GACCTGGCAA GTCCCCTGAG 
TACATCAGCT CCCACAATGG CTGCAGCCCA GCCCACTCGC TTATGGACGG CTACTATGAG 
GATGCAGACA GCAGCTACCC CGCCACCAGG ATGAACGGGG AGCTGAAGAA TTCTTACAAT 
GACTCTGACG CCATGAGCAG CTCCTACGAG TCGTACGATG AGGAGGAGGA GGAAGGGAAA 
GGGCCACAGC CCACACACCA GTGGCCCTCA GAGGAGGCCT CCATGCACCT GGTGAGGGAC 
TGCAGGATAT GTGCCTTCCT GCTGCGGAAA AAGCGCTTCG GGCAGTGGGC CAAGCAGTTG 
ACTGTCATCA AGGAGGACCA GCTCCTGTGT TACAAAAGCT CCAAGGACCG GCAGCCGCAC 
CTGAGGCTAG CGCTGGATGT CTGCAGTGTC ATCTATGTGC CCAAGGACAG CCGGCACAAG 
AGGCACGAGC TGCGCTTCGC CCAGGGGGCT ACTGAAGTCC TGGTGCTGGC CCTGCAGAGC 
CGGGAGCAGG CCGAGGAGTG GCTGAAGGTC ATCCGAGAGG TCAGCAAACC CGTCGGGGGC 
ACTGAGGGGG CAGACGTGCC CAGATCCCCA GTCCTCCTGT GCAAGGCGGA CCTGGACAAG 
AGGCTGTCCC AAGAGAAGCA GACCTCGGAT TCTGACAGCC TAGGCATGGG TGACAGCTGT 
TCCACTCTGG GCCGTGAACA TGGCAAAGGG AAGAAGAGCA GCCTGTCAGA GCTGAAGGGT 
TCAATGAGCA GAGCTGCAGG CCGCAAGATC ACCCGTATCA TCAGCTTCTC TAAGAAGAAG 
GCACTGGCTG ATGACCTGCA GGCGTCCTCT ACTGAGGAGG TTCCGTGCTG TGGTTATCTG 
AATGTGCTGG TGAACCACGG ATGGAAGGAA CGCTGGTGCC GCCTGAAGTG CAACACCTTG 
TATTTCCATA AGGACCGCAC AGACCTGCGG ACCCACGTGA ACGCCATTGC CCTCCGCGGT 
TGTGAGGTGG CCCCAGGCTT TGGGCCCAGA CACCCATTTG CCTTCAGGAT CCTACACAAC 
CGGCAGGAGG TCGCTATCTT AGAGGCAAGC TGCTCAGAGG ACATGGGCCG CTGGCTTGGG 
CTGCTGCTAG TGGAGATGGG CTCCAAAGTC ACTCCCGAAG CATTGCACTA TGACTATGTG 
GATGTGGAGA CCCTAACCAG CATCGTCAGT GCAGGGCGCA ACTCCTTCTT ATATGCAAGA 
TCCTGCCAGG ATCAGTGGCC TGAGCCCCGA GTCTATGATG ATGTTCCTTA TGAAAAGATG 
CAGGACGAGG AGCCTGAGCG TCCCCCTGGA GCCCAGGTCA AGCGCCACGC CTCCACCTGC 
AGTGAGAAGT CCCATCGGGT GGACCCACAG GTCAAAGTCA AGCGCCACGC CTCCAGTGCC 
CATCAGTATA AGTACGGTAA GAACCGAGCT GAGGAGGATG CCAGGAGGTA CCTGGTAGAA 
AAAGAGAAGC TGGAGAAAGA GAAAGAGACA ATCCGGACAG AGCTGATGGC GCTGCGGCAG 
GAAAAGAGGG AACTAAAGGA AGCCATTCGG AACAACCCAG GGGCAAAGTT AAAGGCTCTT 
GAAGAGGCTC TGGCCACCCT CGAAGCTCAG TGTCGGGCAA AGGAGGAGCA CCGGATCGAC 
CTGGAGCTGC GGCTGGTGAC TGTGAAGGAG CGCTTGCAGC AGTCCCTGGC AGGGGGCCCA 
GCCCTGGGGC TCTCCGTCAA CAGCAAGATC AAGAGTGGGG AAACTGCAAA TAAACCCCAG 
AACAATGTCC CAGAGCAACC TCTCCCTGTC AATTGTGTTT CCGAGTTACG GAAGAGGAGC 
CCATCCATCA TAAACTCCAA TCAAGGAAGG GTGCTACAAA AAGCCAAGGA ATGGGAAATG 
AAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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AFAP1L1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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