COL6A6 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL6A6
Entrez Gene ID	131873
Full Name	collagen type VI alpha 6 chain
General protein information	Preferred Names collagen type VI alpha 6 chain Names collagen alpha-6(VI) chain collagen, type VI, alpha 6
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q22.1
Summary	This gene encodes a large protein that contains multiple von Willebrand factor domains and forms a component of the basal lamina of epithelial cells. This protein may regulate epithelial cell-fibronectin interactions. Variation in this gene may be implicated in skin diseases. [provided by RefSeq, May 2017].
Disorder MIM:	616613

mRNA and Protein(s)

mRNA	Protein	Name
NM_001102608.1	NP_001096078.1	collagen alpha-6(VI) chain precursor
XM_017005711.1	XP_016861200.1	collagen alpha-6(VI) chain isoform X3
NM_001102608.2	NP_001096078.1	collagen alpha-6(VI) chain precursor
XM_017005712.2	XP_016861201.1	collagen alpha-6(VI) chain isoform X3
XM_011512425.3	XP_011510727.1	collagen alpha-6(VI) chain isoform X3
XM_017005715.2	XP_016861204.1	collagen alpha-6(VI) chain isoform X3
XM_011512425.2	XP_011510727.1	collagen alpha-6(VI) chain isoform X3
XM_017005715.1	XP_016861204.1	collagen alpha-6(VI) chain isoform X3
XM_011512423.3	XP_011510725.1	collagen alpha-6(VI) chain isoform X1
XM_011512432.2	XP_011510734.1	collagen alpha-6(VI) chain isoform X6
XM_005247121.4	XP_005247178.1	collagen alpha-6(VI) chain isoform X3
XM_017005713.1	XP_016861202.1	collagen alpha-6(VI) chain isoform X3
XM_011512424.2	XP_011510726.1	collagen alpha-6(VI) chain isoform X2
XM_011512426.3	XP_011510728.1	collagen alpha-6(VI) chain isoform X3
XM_011512428.3	XP_011510730.1	collagen alpha-6(VI) chain isoform X3
XM_017005712.1	XP_016861201.1	collagen alpha-6(VI) chain isoform X3
XM_011512428.2	XP_011510730.1	collagen alpha-6(VI) chain isoform X3
XM_017005713.2	XP_016861202.1	collagen alpha-6(VI) chain isoform X3
XM_017005714.2	XP_016861203.1	collagen alpha-6(VI) chain isoform X3
XM_005247121.5	XP_005247178.1	collagen alpha-6(VI) chain isoform X3
XM_011512426.2	XP_011510728.1	collagen alpha-6(VI) chain isoform X3
XM_017005714.1	XP_016861203.1	collagen alpha-6(VI) chain isoform X3
XM_011512423.2	XP_011510725.1	collagen alpha-6(VI) chain isoform X1
XM_017005711.2	XP_016861200.1	collagen alpha-6(VI) chain isoform X3
XM_011512431.2	XP_011510733.1	collagen alpha-6(VI) chain isoform X4
XM_017005716.1	XP_016861205.1	collagen alpha-6(VI) chain isoform X5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL6A6	XP_003310057.1
Bos taurus (cattle)	COL6A6	XP_002685218.2
Gallus gallus (chicken)	COL6A6	XP_426008.4
Xenopus tropicalis (tropical clawed frog)	LOC100486063	XP_002942405.2
Canis lupus familiaris (dog)	COL6A6	XP_853279.3
Mus musculus (house mouse)	Col6a6	NP_001096077.1
	COL6A6	XP_001113364.2
Homo sapiens (human)	COL6A6	NP_001096078.1
Rattus norvegicus (Norway rat)	Col6a6	XP_236593.5
Danio rerio (zebrafish)	col6a6	XP_692457.6

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Naba A, Pearce OMT, Del Rosario A, Ma D, Ding H, Rajeeve V, Cutillas PR, Balkwill FR, Hynes RO
Journal of proteome research16(8)3083-3091(2017 Aug)

Identification of novel candidate variants including COL6A6 polymorphisms in early-onset atopic dermatitis using whole-exome sequencing.
Heo WI, Park KY, Jin T, Lee MK, Kim M, Choi EH, Kim HS, Bae JM, Moon NJ, Seo SJ
BMC medical genetics18(1)8(2017 Jan)

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

New COL6A6 variant detected by whole-exome sequencing is linked to break points in intron 4 and 3'-UTR, deleting exon 5 of RHO, and causing adRP.
de Sousa Dias M, Hernan I, Del?s B, Pascual B, Borr?s E, Gamundi MJ, Ma?? B, Fern?ndez-San Jos? P, Ayuso C, Carballo M
Molecular vision21857-70(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study identified SNPs in COL6A6 associated with atopic dermatitis suggesting that COL6A6 variants may constitute candidate risk factors for early-onset atopic dermatitis.
Title: Identification of novel candidate variants including COL6A6 polymorphisms in early-onset atopic dermatitis using whole-exome sequencing.

A whole-exome sequencing approach led to identification of a deletion in RHO through detection of a new linked variant in COL6A6 in autosomal dominant retinitis pigmentosa.
Title: New COL6A6 variant detected by whole-exome sequencing is linked to break points in intron 4 and 3'-UTR, deleting exon 5 of RHO, and causing adRP.

Reduced collagen VI alpha6 chain expression in the skeletal muscle is associated with collagen VI-related myopathies.
Title: Defective collagen VI ?6 chain expression in the skeletal muscle of patients with collagen VI-related myopathies.

The collagen VI alpha6 chain, but not the alpha5 chain, is up-regulated in dystrophic muscles.
Title: Expression of collagen VI ?5 and ?6 chains in human muscle and in Duchenne muscular dystrophy-related muscle fibrosis.

collagen VI alpha 6 is an important basal lamina component involved in the regulation of epithelial cell behavior most notably as a regulator of epithelial cell-fibronectin interactions
Title: Collagen VI is a basement membrane component that regulates epithelial cell-fibronectin interactions.

The following COL6A6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL6A6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu93806	XM_017005716.1 Latest version!	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu75655	XM_011512431.2 Latest version!	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu75656	XM_011512432.2 Latest version!	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu06178	NM_001102608.2 Latest version!	Homo sapiens collagen type VI alpha 6 chain (COL6A6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_005247121.5 Latest version!	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_011512425.3 Latest version!	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_011512426.3 Latest version!	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_017005713.2 Latest version!	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_017005712.2 Latest version!	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_017005714.2 Latest version!	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_017005711.2 Latest version!	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_011512428.3 Latest version!	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_017005715.2 Latest version!	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu75652	XM_011512424.2 Latest version!	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu75652	XM_011512423.3 Latest version!	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu06178	NM_001102608.1	Homo sapiens collagen type VI alpha 6 chain (COL6A6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_011512425.2	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_017005715.1	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_017005712.1	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_011512428.2	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_017005713.1	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_005247121.4	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_011512426.2	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu06178	XM_017005714.1	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu75652	XM_011512423.2	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu06178	XM_017005711.1	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL6A6 ( XM_017005716.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861205.1 Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu93806
Clone ID Related Accession (Same CDS sequence)	XM_017005716.1
Accession Version	XM_017005716.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTTT CCCCAAGATT TGAAGTTGAA GATTTTTCAG GTCATAATAT GATGTTGCTA 
ATTTTGTTCC TCGTGATAAT TTGTTCCCAT ATTTCTGTGA ACCAAGATTC CGGCCCTGAG 
TATGCAGATG TTGTGTTTTT GGTGGACAGC TCTGATCGCC TGGGATCCAA GTCCTTCCCA 
TTTGTGAAAA TGTTCATCAC CAAAATGATC AGCAGTCTCC CCATAGAGGC CGACAAATAC 
CGTGTGGCCC TGGCCCAGTA CAGTGATAAA CTTCACAGTG AATTCCACCT GAGCACCTTC 
AAAGGCAGGA GCCCCATGCT GAACCACCTA AGGAAGAACT TTGGATTCAT TGGCGGGTCC 
CTGCAGATAG GAAAGGCTCT TCAGGAGGCT CACAGGACTT ATTTCTCTGC ACCCGCAAAT 
GGGAGAGACA AGAAACAGTT TCCCCCAATT CTAGTGGTCC TGGCTTCATC TGAGTCTGAG 
GATAATGTGG AAGAGGCATC AAAGGCCCTG CGGAAAGACG GAGTGAAAAT CATCTCTGTA 
GGGGTGCAGA AAGCTTCTGA GGAAAACCTG AAGGCCATGG CCACGTCTCA GTTTCATTTC 
AACCTTCGGA CAGTCAGAGA CCTCAGCATG TTTTCCCAAA ACATGACACA CATCATCAAG 
GATGTAATAA AGTACAAGGA GGGAGCAGTT GATGACATCT TTGTAGAAGC TTGCCAAGGC 
CCTTCTATGG CCGATGTTGT GTTCCTATTG GATATGTCAA TCAATGGAAG TGAGGAGAAC 
TTTGACTATC TTAAAGGATT CTTGGAAGAA AGTGTATCTG CCCTTGACAT AAAGGAAAAT 
TGCATGAGGG TTGGCCTTGT GGCCTATAGC AATGAGACAA AAGTGATAAA TTCACTGAGC 
ATGGGCATAA ATAAGTCAGA GGTTCTCCAG CATATACAGA ACCTTTCTCC CCGAACTGGG 
AAGGCCTATA CTGGAGCTGC CATCAAAAAG CTCAGGAAGG AAGTTTTTAG TGCACGGAAT 
GGCAGTCGGA AGAATCAGGG GGTGCCCCAG ATTGCCGTGC TGGTGACCCA CCGAGATTCA 
GAAGACAACG TGACAAAAGC AGCTGTTAAC CTCCGACGGG AGGGTGTGAC CATCTTCACC 
CTGGGCATAG AGGGCGCCAG CGACACCCAG TTGGAAAAGA TAGCATCCCA CCCTGCTGAG 
CAGTATGTCT CCAAACTGAA GACCTTCGCT GACCTGGCTG CTCACAACCA GACATTTCTG 
AAGAAGCTGC GGAACCAAAT AACACACACA GTCTCTGTCT TTTCAGAGAG GACTGAAACG 
CTCAAATCTG GTTGTGTGGA CACTGAGGAA GCAGACATCT ATCTGCTTAT CGATGGCTCA 
GGGAGCACCC AGGCCACAGA TTTCCATGAA ATGAAGACGT TCCTGTCAGA GGTGGTAGGG 
ATGTTCAACA TTGCTCCCCA TAAGGTGCGG GTTGGGGCCG TTCAGTATGC TGACAGCTGG 
GACTTGGAAT TTGAGATCAA TAAATACTCC AACAAGCAGG ATTTGGGAAA GGCCATTGAG 
AATATCAGGC AGATGGGTGG GAATACAAAC ACAGGCGCAG CACTGAATTT CACACTGAGT 
CTGTTGCAAA AAGCAAAGAA GCAGCGAGGA AACAAAGTTC CATGCCACCT TGTTGTCCTG 
ACAAATGGCA TGTCCAAGGA TAGCATCTTG GAGCCTGCAA ACAGACTGAG AGAAGAGCAC 
ATCCGAGTTT ATGCTATCGG GATCAAGGAG GCCAACCAAA CACAGCTGAG AGAAATTGCA 
GGAGAGGAAA AGAGAGTGTA TTACGTGCAT GACTTTGATG CATTGAAAGA CATAAGAAAC 
CAAGTTGTTC AAGAAATCTG TACTGAAGAA GCTTGCAAAG AGATGAAAGC TGACATCATG 
TTTCTGGTGG ACAGTTCTGG AAGTATAGGA CCTGAAAACT TCAGCAAAAT GAAAACATTT 
ATGAAAAACC TGGTGAGCAA GTCTCAGATT GGACCAGATC GGGTGCAAAT TGGTGTAGTC 
CAGTTCAGCG ACATCAATAA GGAAGAGTTT CAGCTCAACA GATTCATGTC CCAAAGCGAC 
ATTTCAAATG CAATAGACCA AATGGCTCAC ATTGGACAAA CCACCCTGAC TGGTAGTGCC 
CTGAGCTTTG TGTCTCAGTA CTTCAGCCCC ACCAAGGGCG CCCGGCCCAA CATCAGAAAG 
TTTCTCATCC TCATCACGGA TGGTGAAGCT CAGGACATAG TAAAGGAACC AGCAGTAGTG 
CTTCGGCAAG AAGGTGTAAT CATCTATTCT GTGGGAGTGT TTGGCTCCAA TGTCACCCAG 
CTTGAGGAGA TCAGTGGGAG GCCCGAGATG GTTTTTTATG TTGAGAATTT TGACATTCTG 
CAGCGCATTG AAGATGATCT TGTTTTTGGA ATATGCAGCC CCCGTGAAGA ATGCAAGCGG 
ATTGAAGTTT TAGACGTTGT GTTTGTCATT GATAGCTCTG GCAGTATTGA CTATGATGAG 
TATAATATCA TGAAGGATTT TATGATTGGC TTAGTGAAAA AAGCTGATGT GGGCAAGAAT 
CAGGTCCGGT TTGGGGCTCT GAAGTATGCT GATGACCCAG AGGTGCTGTT TTATCTGGAT 
GACTTTGGCA CAAAACTGGA GGTAATTTCA GTGCTCCAGA ATGACCAAGC CATGGGTGGC 
AGTACTTATA CTGCTGAGGC ACTGGGCTTC TCAGACCACA TGTTCACTGA AGCCCGGGGC 
AGCCGCCTGA ACAAGGGGGT CCCCCAAGTC CTCATTGTGA TCACCGATGG GGAATCCCAT 
GATGCTGATA AACTCAATGC CACGGCAAAG GCCTTGCGGG ACAAAGGCAT TCTTGTCCTG 
GCTGTGGGGA TTGATGGTGC CAATCCCGTG GAGCTGTTAG CCATGGCAGG ATCAAGCGAC 
AAGTACTTCT TCGTGGAGAC TTTTGGAGGT CTGAAGGGAA TATTTTCAGA TGTGACAGCC 
AGTGTCTGCA ACTCTTCAAA AGTAGATTGT GAAATTGACA AAGTAGATCT TGTTTTCCTT 
ATGGATGGTT CAACTAGCAT TCAGCCAAAT GACTTCAAGA AAATGAAGGA ATTTCTGGCA 
TCTGTTGTTC AAGACTTTGA TGTCAGCCTC AACAGAGTGC GAATAGGAGC GGCCCAGTTT 
AGCGATACCT ATCACCCGGA GTTTCCACTG GGAACTTTCA TAGGTGAAAA AGAGATATCA 
TTTCAGATTG AAAACATCAA GCAGATCTTT GGAAACACAC ACATCGGTGC TGCACTCAGG 
GAGGTGGAAC ATTACTTCAG GCCAGACATG GGCAGCAGGA TAAATACAGG TACCCCACAG 
GTGCTGCTGG TCCTTACAGA TGGCCAGTCC CAAGACGAGG TGGCCCAGGC CGCGGAAGCC 
CTGAGACACA GAGGTATCGA CATCTACTCC GTGGGCATTG GGGATGTGGA TGACCAGCAG 
CTCATTCAGA TCACCGGGAC TGCAGAGAAA AAACTGACAG TGCACAACTT CGATGAACTG 
AAGAAGGTCA ATAAAAGGAT CGTTCGCAAC ATCTGTACCA CAGCGGGTGA AAGCAACTGT 
TTCGTGGATG TTGTGGTGGG ATTTGATGTC TCAACTCAGG AGAAAGGGCA GACTTTGCTT 
GAAGGTCAGC CTTGGATGGA AACCTACCTT CAAGACATCT TACGTGCCAT CAGCTCCCTC 
AATGGAGTAA GCTGTGAGGT GGGCACAGAG ACTCAGGTCA GTGTGGCTTT TCAAGTGACC 
AATGCCATGG AAAAATATTC TCCCAAGTTT GAGATCTACA GTGAAAACAT ACTGAATAGC 
TTGAAGGATA TAACAGTTAA AGGACCATCT CTTCTCAATG CAAACCTCTT GGATTCTCTA 
TGGGATACAT TTCAGAATAA ATCAGCTGCT CGAGGAAAGG TGGTCCTTTT ATTTTCAGAT 
GGATTGGATG ATGATGTTGA GAAACTTGAA CAAAAATCTG ATGAACTTAG AAAAGAAGGC 
CTGAATGCCC TCATAACTGT TGCTCTGGAT GGACCTGCTG ATTCAAGTGA CTTGGCTGAT 
CTTCCCTATA TTGAATTTGG GAAAGGATTT GAGTACAGGA CACAGCTCTC TATTGGCATG 
AGAGAACTTG GAAGCCGGCT GTCAAAGCAG CTGGTCAATG TTGCTGAAAG GACATGCTGC 
TGTTTGTTCT GCAAGTGCAT TGGAGGAGAT GGCACAATGG GAGATCCTGG ACCACCAGGG 
AAAAGGGGAC CTCCAGGTTT TAAAGGCAGT GAAGGCTACC TGGGAGAGGA GGGAATCGCT 
GGAGAAAGAG GAGCCCCTGG ACCAGTGGGA GAGCAAGGTA CTAAGGGATG CTATGGCACC 
AAAGGTCCTA AGGGAAACAG AGGACTAAAT GGACAGGAGG GAGAAGTTGG GGAAAATGGA 
ATTGACGGAT TAAACGGAGA ACAGGGTGAT AATGGTCTTC CTGGAAGAAA AGGAGAAAAG 
GGAGATGAGG GATCTCAGGG AAGCCCAGGG AAGAGAGGGA CTCCTGGTGA CCGTGGAGCA 
AAGGGCCTGC GAGGGGATCC CGGAGCTCCT GGAGTTGACA GTAGCATAGA AGGACCCACA 
GGCTTGAAAG GAGAACGTGG AAGACAAGGC AGAAGAGGCT GGCCAGGCCC CCCCGGGACA 
CCAGGCTCCA GAAGAAAGAC AGCAGCTCAT GGCAGAAGGG GACATACAGG CCCACAGGGA 
ACAGCAGGCA TCCCAGGACC AGATGGACTT GAAGGCTCCC TGGGACTTAA GGGCCCTCAG 
GGCCCAAGAG GAGAGGCTGG TGTGAAAGGA GAAAAAGGAG GTGTGGGAAG TAAAGGTCCC 
CAGGGGCCTC CAGGACCCGG AGGAGAGGCA GGGAATCAAG GCCGTTTGGG AAGCCAAGGA 
AATAAAGGAG AACCTGGAGA TCTGGGAGAA AAAGGAGCTG TTGGCTTTCC TGGTCCTCGT 
GGCTTGCAGG GCAATGATGG CAGTCCAGGT TATGGTAGTG TCGGACGCAA GGGAGCAAAG 
GGACAAGAAG GATTCCCTGG AGAAAGTGGA CCTAAGGGTG AGATTGGGGA CCCTGGTGGT 
CCAGGAGAGA CTGGGCTGAA GGGAGCTAGA GGCAAAATGA TGAGGCAAGT GTTCTCTCCA 
ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861205.1
CDS	209..5374
Translation	MEVSPRFEVEDFSGHNMMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMMRQVFSPT

Target ORF information:

RefSeq Version	XM_017005716.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005716.1

ORF Insert Sequence:

ATGGAAGTTT CCCCAAGATT TGAAGTTGAA GATTTTTCAG GTCATAATAT GATGTTGCTA 
ATTTTGTTCC TCGTGATAAT TTGTTCCCAT ATTTCTGTGA ACCAAGATTC CGGCCCTGAG 
TATGCAGATG TTGTGTTTTT GGTGGACAGC TCTGATCGCC TGGGATCCAA GTCCTTCCCA 
TTTGTGAAAA TGTTCATCAC CAAAATGATC AGCAGTCTCC CCATAGAGGC CGACAAATAC 
CGTGTGGCCC TGGCCCAGTA CAGTGATAAA CTTCACAGTG AATTCCACCT GAGCACCTTC 
AAAGGCAGGA GCCCCATGCT GAACCACCTA AGGAAGAACT TTGGATTCAT TGGCGGGTCC 
CTGCAGATAG GAAAGGCTCT TCAGGAGGCT CACAGGACTT ATTTCTCTGC ACCCGCAAAT 
GGGAGAGACA AGAAACAGTT TCCCCCAATT CTAGTGGTCC TGGCTTCATC TGAGTCTGAG 
GATAATGTGG AAGAGGCATC AAAGGCCCTG CGGAAAGACG GAGTGAAAAT CATCTCTGTA 
GGGGTGCAGA AAGCTTCTGA GGAAAACCTG AAGGCCATGG CCACGTCTCA GTTTCATTTC 
AACCTTCGGA CAGTCAGAGA CCTCAGCATG TTTTCCCAAA ACATGACACA CATCATCAAG 
GATGTAATAA AGTACAAGGA GGGAGCAGTT GATGACATCT TTGTAGAAGC TTGCCAAGGC 
CCTTCTATGG CCGATGTTGT GTTCCTATTG GATATGTCAA TCAATGGAAG TGAGGAGAAC 
TTTGACTATC TTAAAGGATT CTTGGAAGAA AGTGTATCTG CCCTTGACAT AAAGGAAAAT 
TGCATGAGGG TTGGCCTTGT GGCCTATAGC AATGAGACAA AAGTGATAAA TTCACTGAGC 
ATGGGCATAA ATAAGTCAGA GGTTCTCCAG CATATACAGA ACCTTTCTCC CCGAACTGGG 
AAGGCCTATA CTGGAGCTGC CATCAAAAAG CTCAGGAAGG AAGTTTTTAG TGCACGGAAT 
GGCAGTCGGA AGAATCAGGG GGTGCCCCAG ATTGCCGTGC TGGTGACCCA CCGAGATTCA 
GAAGACAACG TGACAAAAGC AGCTGTTAAC CTCCGACGGG AGGGTGTGAC CATCTTCACC 
CTGGGCATAG AGGGCGCCAG CGACACCCAG TTGGAAAAGA TAGCATCCCA CCCTGCTGAG 
CAGTATGTCT CCAAACTGAA GACCTTCGCT GACCTGGCTG CTCACAACCA GACATTTCTG 
AAGAAGCTGC GGAACCAAAT AACACACACA GTCTCTGTCT TTTCAGAGAG GACTGAAACG 
CTCAAATCTG GTTGTGTGGA CACTGAGGAA GCAGACATCT ATCTGCTTAT CGATGGCTCA 
GGGAGCACCC AGGCCACAGA TTTCCATGAA ATGAAGACGT TCCTGTCAGA GGTGGTAGGG 
ATGTTCAACA TTGCTCCCCA TAAGGTGCGG GTTGGGGCCG TTCAGTATGC TGACAGCTGG 
GACTTGGAAT TTGAGATCAA TAAATACTCC AACAAGCAGG ATTTGGGAAA GGCCATTGAG 
AATATCAGGC AGATGGGTGG GAATACAAAC ACAGGCGCAG CACTGAATTT CACACTGAGT 
CTGTTGCAAA AAGCAAAGAA GCAGCGAGGA AACAAAGTTC CATGCCACCT TGTTGTCCTG 
ACAAATGGCA TGTCCAAGGA TAGCATCTTG GAGCCTGCAA ACAGACTGAG AGAAGAGCAC 
ATCCGAGTTT ATGCTATCGG GATCAAGGAG GCCAACCAAA CACAGCTGAG AGAAATTGCA 
GGAGAGGAAA AGAGAGTGTA TTACGTGCAT GACTTTGATG CATTGAAAGA CATAAGAAAC 
CAAGTTGTTC AAGAAATCTG TACTGAAGAA GCTTGCAAAG AGATGAAAGC TGACATCATG 
TTTCTGGTGG ACAGTTCTGG AAGTATAGGA CCTGAAAACT TCAGCAAAAT GAAAACATTT 
ATGAAAAACC TGGTGAGCAA GTCTCAGATT GGACCAGATC GGGTGCAAAT TGGTGTAGTC 
CAGTTCAGCG ACATCAATAA GGAAGAGTTT CAGCTCAACA GATTCATGTC CCAAAGCGAC 
ATTTCAAATG CAATAGACCA AATGGCTCAC ATTGGACAAA CCACCCTGAC TGGTAGTGCC 
CTGAGCTTTG TGTCTCAGTA CTTCAGCCCC ACCAAGGGCG CCCGGCCCAA CATCAGAAAG 
TTTCTCATCC TCATCACGGA TGGTGAAGCT CAGGACATAG TAAAGGAACC AGCAGTAGTG 
CTTCGGCAAG AAGGTGTAAT CATCTATTCT GTGGGAGTGT TTGGCTCCAA TGTCACCCAG 
CTTGAGGAGA TCAGTGGGAG GCCCGAGATG GTTTTTTATG TTGAGAATTT TGACATTCTG 
CAGCGCATTG AAGATGATCT TGTTTTTGGA ATATGCAGCC CCCGTGAAGA ATGCAAGCGG 
ATTGAAGTTT TAGACGTTGT GTTTGTCATT GATAGCTCTG GCAGTATTGA CTATGATGAG 
TATAATATCA TGAAGGATTT TATGATTGGC TTAGTGAAAA AAGCTGATGT GGGCAAGAAT 
CAGGTCCGGT TTGGGGCTCT GAAGTATGCT GATGACCCAG AGGTGCTGTT TTATCTGGAT 
GACTTTGGCA CAAAACTGGA GGTAATTTCA GTGCTCCAGA ATGACCAAGC CATGGGTGGC 
AGTACTTATA CTGCTGAGGC ACTGGGCTTC TCAGACCACA TGTTCACTGA AGCCCGGGGC 
AGCCGCCTGA ACAAGGGGGT CCCCCAAGTC CTCATTGTGA TCACCGATGG GGAATCCCAT 
GATGCTGATA AACTCAATGC CACGGCAAAG GCCTTGCGGG ACAAAGGCAT TCTTGTCCTG 
GCTGTGGGGA TTGATGGTGC CAATCCCGTG GAGCTGTTAG CCATGGCAGG ATCAAGCGAC 
AAGTACTTCT TCGTGGAGAC TTTTGGAGGT CTGAAGGGAA TATTTTCAGA TGTGACAGCC 
AGTGTCTGCA ACTCTTCAAA AGTAGATTGT GAAATTGACA AAGTAGATCT TGTTTTCCTT 
ATGGATGGTT CAACTAGCAT TCAGCCAAAT GACTTCAAGA AAATGAAGGA ATTTCTGGCA 
TCTGTTGTTC AAGACTTTGA TGTCAGCCTC AACAGAGTGC GAATAGGAGC GGCCCAGTTT 
AGCGATACCT ATCACCCGGA GTTTCCACTG GGAACTTTCA TAGGTGAAAA AGAGATATCA 
TTTCAGATTG AAAACATCAA GCAGATCTTT GGAAACACAC ACATCGGTGC TGCACTCAGG 
GAGGTGGAAC ATTACTTCAG GCCAGACATG GGCAGCAGGA TAAATACAGG TACCCCACAG 
GTGCTGCTGG TCCTTACAGA TGGCCAGTCC CAAGACGAGG TGGCCCAGGC CGCGGAAGCC 
CTGAGACACA GAGGTATCGA CATCTACTCC GTGGGCATTG GGGATGTGGA TGACCAGCAG 
CTCATTCAGA TCACCGGGAC TGCAGAGAAA AAACTGACAG TGCACAACTT CGATGAACTG 
AAGAAGGTCA ATAAAAGGAT CGTTCGCAAC ATCTGTACCA CAGCGGGTGA AAGCAACTGT 
TTCGTGGATG TTGTGGTGGG ATTTGATGTC TCAACTCAGG AGAAAGGGCA GACTTTGCTT 
GAAGGTCAGC CTTGGATGGA AACCTACCTT CAAGACATCT TACGTGCCAT CAGCTCCCTC 
AATGGAGTAA GCTGTGAGGT GGGCACAGAG ACTCAGGTCA GTGTGGCTTT TCAAGTGACC 
AATGCCATGG AAAAATATTC TCCCAAGTTT GAGATCTACA GTGAAAACAT ACTGAATAGC 
TTGAAGGATA TAACAGTTAA AGGACCATCT CTTCTCAATG CAAACCTCTT GGATTCTCTA 
TGGGATACAT TTCAGAATAA ATCAGCTGCT CGAGGAAAGG TGGTCCTTTT ATTTTCAGAT 
GGATTGGATG ATGATGTTGA GAAACTTGAA CAAAAATCTG ATGAACTTAG AAAAGAAGGC 
CTGAATGCCC TCATAACTGT TGCTCTGGAT GGACCTGCTG ATTCAAGTGA CTTGGCTGAT 
CTTCCCTATA TTGAATTTGG GAAAGGATTT GAGTACAGGA CACAGCTCTC TATTGGCATG 
AGAGAACTTG GAAGCCGGCT GTCAAAGCAG CTGGTCAATG TTGCTGAAAG GACATGCTGC 
TGTTTGTTCT GCAAGTGCAT TGGAGGAGAT GGCACAATGG GAGATCCTGG ACCACCAGGG 
AAAAGGGGAC CTCCAGGTTT TAAAGGCAGT GAAGGCTACC TGGGAGAGGA GGGAATCGCT 
GGAGAAAGAG GAGCCCCTGG ACCAGTGGGA GAGCAAGGTA CTAAGGGATG CTATGGCACC 
AAAGGTCCTA AGGGAAACAG AGGACTAAAT GGACAGGAGG GAGAAGTTGG GGAAAATGGA 
ATTGACGGAT TAAACGGAGA ACAGGGTGAT AATGGTCTTC CTGGAAGAAA AGGAGAAAAG 
GGAGATGAGG GATCTCAGGG AAGCCCAGGG AAGAGAGGGA CTCCTGGTGA CCGTGGAGCA 
AAGGGCCTGC GAGGGGATCC CGGAGCTCCT GGAGTTGACA GTAGCATAGA AGGACCCACA 
GGCTTGAAAG GAGAACGTGG AAGACAAGGC AGAAGAGGCT GGCCAGGCCC CCCCGGGACA 
CCAGGCTCCA GAAGAAAGAC AGCAGCTCAT GGCAGAAGGG GACATACAGG CCCACAGGGA 
ACAGCAGGCA TCCCAGGACC AGATGGACTT GAAGGCTCCC TGGGACTTAA GGGCCCTCAG 
GGCCCAAGAG GAGAGGCTGG TGTGAAAGGA GAAAAAGGAG GTGTGGGAAG TAAAGGTCCC 
CAGGGGCCTC CAGGACCCGG AGGAGAGGCA GGGAATCAAG GCCGTTTGGG AAGCCAAGGA 
AATAAAGGAG AACCTGGAGA TCTGGGAGAA AAAGGAGCTG TTGGCTTTCC TGGTCCTCGT 
GGCTTGCAGG GCAATGATGG CAGTCCAGGT TATGGTAGTG TCGGACGCAA GGGAGCAAAG 
GGACAAGAAG GATTCCCTGG AGAAAGTGGA CCTAAGGGTG AGATTGGGGA CCCTGGTGGT 
CCAGGAGAGA CTGGGCTGAA GGGAGCTAGA GGCAAAATGA TGAGGCAAGT GTTCTCTCCA 
ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_011512431.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510733.1 Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X13, mRNA.

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CloneID	OHu75655
Clone ID Related Accession (Same CDS sequence)	XM_011512431.2
Accession Version	XM_011512431.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTTT CCCCAAGATT TGAAGTTGAA GATTTTTCAG GTCATAATAT GATGTTGCTA 
ATTTTGTTCC TCGTGATAAT TTGTTCCCAT ATTTCTGTGA ACCAAGATTC CGGCCCTGAG 
TATGCAGATG TTGTGTTTTT GGTGGACAGC TCTGATCGCC TGGGATCCAA GTCCTTCCCA 
TTTGTGAAAA TGTTCATCAC CAAAATGATC AGCAGTCTCC CCATAGAGGC CGACAAATAC 
CGTGTGGCCC TGGCCCAGTA CAGTGATAAA CTTCACAGTG AATTCCACCT GAGCACCTTC 
AAAGGCAGGA GCCCCATGCT GAACCACCTA AGGAAGAACT TTGGATTCAT TGGCGGGTCC 
CTGCAGATAG GAAAGGCTCT TCAGGAGGCT CACAGGACTT ATTTCTCTGC ACCCGCAAAT 
GGGAGAGACA AGAAACAGTT TCCCCCAATT CTAGTGGTCC TGGCTTCATC TGAGTCTGAG 
GATAATGTGG AAGAGGCATC AAAGGCCCTG CGGAAAGACG GAGTGAAAAT CATCTCTGTA 
GGGGTGCAGA AAGCTTCTGA GGAAAACCTG AAGGCCATGG CCACGTCTCA GTTTCATTTC 
AACCTTCGGA CAGTCAGAGA CCTCAGCATG TTTTCCCAAA ACATGACACA CATCATCAAG 
GATGTAATAA AGTACAAGGA GGGAGCAGTT GATGACATCT TTGTAGAAGC TTGCCAAGGC 
CCTTCTATGG CCGATGTTGT GTTCCTATTG GATATGTCAA TCAATGGAAG TGAGGAGAAC 
TTTGACTATC TTAAAGGATT CTTGGAAGAA AGTGTATCTG CCCTTGACAT AAAGGAAAAT 
TGCATGAGGG TTGGCCTTGT GGCCTATAGC AATGAGACAA AAGTGATAAA TTCACTGAGC 
ATGGGCATAA ATAAGTCAGA GGTTCTCCAG CATATACAGA ACCTTTCTCC CCGAACTGGG 
AAGGCCTATA CTGGAGCTGC CATCAAAAAG CTCAGGAAGG AAGTTTTTAG TGCACGGAAT 
GGCAGTCGGA AGAATCAGGG GGTGCCCCAG ATTGCCGTGC TGGTGACCCA CCGAGATTCA 
GAAGACAACG TGACAAAAGC AGCTGTTAAC CTCCGACGGG AGGGTGTGAC CATCTTCACC 
CTGGGCATAG AGGGCGCCAG CGACACCCAG TTGGAAAAGA TAGCATCCCA CCCTGCTGAG 
CAGTATGTCT CCAAACTGAA GACCTTCGCT GACCTGGCTG CTCACAACCA GACATTTCTG 
AAGAAGCTGC GGAACCAAAT AACACACACA GTCTCTGTCT TTTCAGAGAG GACTGAAACG 
CTCAAATCTG GTTGTGTGGA CACTGAGGAA GCAGACATCT ATCTGCTTAT CGATGGCTCA 
GGGAGCACCC AGGCCACAGA TTTCCATGAA ATGAAGACGT TCCTGTCAGA GGTGGTAGGG 
ATGTTCAACA TTGCTCCCCA TAAGGTGCGG GTTGGGGCCG TTCAGTATGC TGACAGCTGG 
GACTTGGAAT TTGAGATCAA TAAATACTCC AACAAGCAGG ATTTGGGAAA GGCCATTGAG 
AATATCAGGC AGATGGGTGG GAATACAAAC ACAGGCGCAG CACTGAATTT CACACTGAGT 
CTGTTGCAAA AAGCAAAGAA GCAGCGAGGA AACAAAGTTC CATGCCACCT TGTTGTCCTG 
ACAAATGGCA TGTCCAAGGA TAGCATCTTG GAGCCTGCAA ACAGACTGAG AGAAGAGCAC 
ATCCGAGTTT ATGCTATCGG GATCAAGGAG GCCAACCAAA CACAGCTGAG AGAAATTGCA 
GGAGAGGAAA AGAGAGTGTA TTACGTGCAT GACTTTGATG CATTGAAAGA CATAAGAAAC 
CAAGTTGTTC AAGAAATCTG TACTGAAGAA GCTTGCAAAG AGATGAAAGC TGACATCATG 
TTTCTGGTGG ACAGTTCTGG AAGTATAGGA CCTGAAAACT TCAGCAAAAT GAAAACATTT 
ATGAAAAACC TGGTGAGCAA GTCTCAGATT GGACCAGATC GGGTGCAAAT TGGTGTAGTC 
CAGTTCAGCG ACATCAATAA GGAAGAGTTT CAGCTCAACA GATTCATGTC CCAAAGCGAC 
ATTTCAAATG CAATAGACCA AATGGCTCAC ATTGGACAAA CCACCCTGAC TGGTAGTGCC 
CTGAGCTTTG TGTCTCAGTA CTTCAGCCCC ACCAAGGGCG CCCGGCCCAA CATCAGAAAG 
TTTCTCATCC TCATCACGGA TGGTGAAGCT CAGGACATAG TAAAGGAACC AGCAGTAGTG 
CTTCGGCAAG AAGGTGTAAT CATCTATTCT GTGGGAGTGT TTGGCTCCAA TGTCACCCAG 
CTTGAGGAGA TCAGTGGGAG GCCCGAGATG GTTTTTTATG TTGAGAATTT TGACATTCTG 
CAGCGCATTG AAGATGATCT TGTTTTTGGA ATATGCAGCC CCCGTGAAGA ATGCAAGCGG 
ATTGAAGTTT TAGACGTTGT GTTTGTCATT GATAGCTCTG GCAGTATTGA CTATGATGAG 
TATAATATCA TGAAGGATTT TATGATTGGC TTAGTGAAAA AAGCTGATGT GGGCAAGAAT 
CAGGTCCGGT TTGGGGCTCT GAAGTATGCT GATGACCCAG AGGTGCTGTT TTATCTGGAT 
GACTTTGGCA CAAAACTGGA GGTAATTTCA GTGCTCCAGA ATGACCAAGC CATGGGTGGC 
AGTACTTATA CTGCTGAGGC ACTGGGCTTC TCAGACCACA TGTTCACTGA AGCCCGGGGC 
AGCCGCCTGA ACAAGGGGGT CCCCCAAGTC CTCATTGTGA TCACCGATGG GGAATCCCAT 
GATGCTGATA AACTCAATGC CACGGCAAAG GCCTTGCGGG ACAAAGGCAT TCTTGTCCTG 
GCTGTGGGGA TTGATGGTGC CAATCCCGTG GAGCTGTTAG CCATGGCAGG ATCAAGCGAC 
AAGTACTTCT TCGTGGAGAC TTTTGGAGGT CTGAAGGGAA TATTTTCAGA TGTGACAGCC 
AGTGTCTGCA ACTCTTCAAA AGTAGATTGT GAAATTGACA AAGTAGATCT TGTTTTCCTT 
ATGGATGGTT CAACTAGCAT TCAGCCAAAT GACTTCAAGA AAATGAAGGA ATTTCTGGCA 
TCTGTTGTTC AAGACTTTGA TGTCAGCCTC AACAGAGTGC GAATAGGAGC GGCCCAGTTT 
AGCGATACCT ATCACCCGGA GTTTCCACTG GGAACTTTCA TAGGTGAAAA AGAGATATCA 
TTTCAGATTG AAAACATCAA GCAGATCTTT GGAAACACAC ACATCGGTGC TGCACTCAGG 
GAGGTGGAAC ATTACTTCAG GCCAGACATG GGCAGCAGGA TAAATACAGG TACCCCACAG 
GTGCTGCTGG TCCTTACAGA TGGCCAGTCC CAAGACGAGG TGGCCCAGGC CGCGGAAGCC 
CTGAGACACA GAGGTATCGA CATCTACTCC GTGGGCATTG GGGATGTGGA TGACCAGCAG 
CTCATTCAGA TCACCGGGAC TGCAGAGAAA AAACTGACAG TGCACAACTT CGATGAACTG 
AAGAAGGTCA ATAAAAGGAT CGTTCGCAAC ATCTGTACCA CAGCGGGTGA AAGCAACTGT 
TTCGTGGATG TTGTGGTGGG ATTTGATGTC TCAACTCAGG AGAAAGGGCA GACTTTGCTT 
GAAGGTCAGC CTTGGATGGA AACCTACCTT CAAGACATCT TACGTGCCAT CAGCTCCCTC 
AATGGAGTAA GCTGTGAGGT GGGCACAGAG ACTCAGGTCA GTGTGGCTTT TCAAGTGACC 
AATGCCATGG AAAAATATTC TCCCAAGTTT GAGATCTACA GTGAAAACAT ACTGAATAGC 
TTGAAGGATA TAACAGTTAA AGGACCATCT CTTCTCAATG CAAACCTCTT GGATTCTCTA 
TGGGATACAT TTCAGAATAA ATCAGCTGCT CGAGGAAAGG TGGTCCTTTT ATTTTCAGAT 
GGATTGGATG ATGATGTTGA GAAACTTGAA CAAAAATCTG ATGAACTTAG AAAAGAAGGC 
CTGAATGCCC TCATAACTGT TGCTCTGGAT GGACCTGCTG ATTCAAGTGA CTTGGCTGAT 
CTTCCCTATA TTGAATTTGG GAAAGGATTT GAGTACAGGA CACAGCTCTC TATTGGCATG 
AGAGAACTTG GAAGCCGGCT GTCAAAGCAG CTGGTCAATG TTGCTGAAAG GACATGCTGC 
TGTTTGTTCT GCAAGTGCAT TGGAGGAGAT GGCACAATGG GAGATCCTGG ACCACCAGGG 
AAAAGGGGAC CTCCAGGTTT TAAAGGCAGT GAAGGCTACC TGGGAGAGGA GGGAATCGCT 
GGAGAAAGAG GAGCCCCTGG ACCAGTGGGA GAGCAAGGTA CTAAGGGATG CTATGGCACC 
AAAGGTCCTA AGGGAAACAG AGGACTAAAT GGACAGGAGG GAGAAGTTGG GGAAAATGGA 
ATTGACGGAT TAAACGGAGA ACAGGGTGAT AATGGTCTTC CTGGAAGAAA AGGAGAAAAG 
GGAGATGAGG GATCTCAGGG AAGCCCAGGG AAGAGAGGGA CTCCTGGTGA CCGTGGAGCA 
AAGGGCCTGC GAGGGGATCC CGGAGCTCCT GGAGTTGACA GTAGCATAGA AGGACCCACA 
GGCTTGAAAG GAGAACGTGG AAGACAAGGC AGAAGAGGCT GGCCAGGCCC CCCCGGGACA 
CCAGGCTCCA GAAGAAAGAC AGCAGCTCAT GGCAGAAGGG GACATACAGG CCCACAGGGA 
ACAGCAGGCA TCCCAGGACC AGATGGACTT GAAGGCTCCC TGGGACTTAA GGGCCCTCAG 
GGCCCAAGAG GAGAGGCTGG TGTGAAAGGA GAAAAAGGAG GTGTGGGAAG TAAAGGTCCC 
CAGGGGCCTC CAGGACCCGG AGGAGAGGCA GGGAATCAAG GCCGTTTGGG AAGCCAAGGA 
AATAAAGGAG AACCTGGAGA TCTGGGAGAA AAAGGAGCTG TTGGCTTTCC TGGTCCTCGT 
GGCTTGCAGG GCAATGATGG CAGTCCAGGT TATGGTAGTG TCGGACGCAA GGGAGCAAAG 
GGACAAGAAG GATTCCCTGG AGAAAGTGGA CCTAAGGGTG AGATTGGGGA CCCTGGTGGT 
CCAGGAGAGA CTGGGCTGAA GGGAGCTAGA GGCAAAATGA TATCTGCTGG GCTTCCAGGA 
GAGATGGGAT CCCCTGGGGA ACCAGGACCT CCTGGACGTA AGGGTGTGAA AGGAGCCAAA 
GGCTTGGCTT CATTTTCTAC ATGTGAGCTC ATTCAGTATG TGCGAGACCG CAGTCCTGGC 
AGGCATGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510733.1
CDS	209..5500
Translation	MEVSPRFEVEDFSGHNMMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHD

Target ORF information:

RefSeq Version	XM_011512431.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512431.2

ORF Insert Sequence:

ATGGAAGTTT CCCCAAGATT TGAAGTTGAA GATTTTTCAG GTCATAATAT GATGTTGCTA 
ATTTTGTTCC TCGTGATAAT TTGTTCCCAT ATTTCTGTGA ACCAAGATTC CGGCCCTGAG 
TATGCAGATG TTGTGTTTTT GGTGGACAGC TCTGATCGCC TGGGATCCAA GTCCTTCCCA 
TTTGTGAAAA TGTTCATCAC CAAAATGATC AGCAGTCTCC CCATAGAGGC CGACAAATAC 
CGTGTGGCCC TGGCCCAGTA CAGTGATAAA CTTCACAGTG AATTCCACCT GAGCACCTTC 
AAAGGCAGGA GCCCCATGCT GAACCACCTA AGGAAGAACT TTGGATTCAT TGGCGGGTCC 
CTGCAGATAG GAAAGGCTCT TCAGGAGGCT CACAGGACTT ATTTCTCTGC ACCCGCAAAT 
GGGAGAGACA AGAAACAGTT TCCCCCAATT CTAGTGGTCC TGGCTTCATC TGAGTCTGAG 
GATAATGTGG AAGAGGCATC AAAGGCCCTG CGGAAAGACG GAGTGAAAAT CATCTCTGTA 
GGGGTGCAGA AAGCTTCTGA GGAAAACCTG AAGGCCATGG CCACGTCTCA GTTTCATTTC 
AACCTTCGGA CAGTCAGAGA CCTCAGCATG TTTTCCCAAA ACATGACACA CATCATCAAG 
GATGTAATAA AGTACAAGGA GGGAGCAGTT GATGACATCT TTGTAGAAGC TTGCCAAGGC 
CCTTCTATGG CCGATGTTGT GTTCCTATTG GATATGTCAA TCAATGGAAG TGAGGAGAAC 
TTTGACTATC TTAAAGGATT CTTGGAAGAA AGTGTATCTG CCCTTGACAT AAAGGAAAAT 
TGCATGAGGG TTGGCCTTGT GGCCTATAGC AATGAGACAA AAGTGATAAA TTCACTGAGC 
ATGGGCATAA ATAAGTCAGA GGTTCTCCAG CATATACAGA ACCTTTCTCC CCGAACTGGG 
AAGGCCTATA CTGGAGCTGC CATCAAAAAG CTCAGGAAGG AAGTTTTTAG TGCACGGAAT 
GGCAGTCGGA AGAATCAGGG GGTGCCCCAG ATTGCCGTGC TGGTGACCCA CCGAGATTCA 
GAAGACAACG TGACAAAAGC AGCTGTTAAC CTCCGACGGG AGGGTGTGAC CATCTTCACC 
CTGGGCATAG AGGGCGCCAG CGACACCCAG TTGGAAAAGA TAGCATCCCA CCCTGCTGAG 
CAGTATGTCT CCAAACTGAA GACCTTCGCT GACCTGGCTG CTCACAACCA GACATTTCTG 
AAGAAGCTGC GGAACCAAAT AACACACACA GTCTCTGTCT TTTCAGAGAG GACTGAAACG 
CTCAAATCTG GTTGTGTGGA CACTGAGGAA GCAGACATCT ATCTGCTTAT CGATGGCTCA 
GGGAGCACCC AGGCCACAGA TTTCCATGAA ATGAAGACGT TCCTGTCAGA GGTGGTAGGG 
ATGTTCAACA TTGCTCCCCA TAAGGTGCGG GTTGGGGCCG TTCAGTATGC TGACAGCTGG 
GACTTGGAAT TTGAGATCAA TAAATACTCC AACAAGCAGG ATTTGGGAAA GGCCATTGAG 
AATATCAGGC AGATGGGTGG GAATACAAAC ACAGGCGCAG CACTGAATTT CACACTGAGT 
CTGTTGCAAA AAGCAAAGAA GCAGCGAGGA AACAAAGTTC CATGCCACCT TGTTGTCCTG 
ACAAATGGCA TGTCCAAGGA TAGCATCTTG GAGCCTGCAA ACAGACTGAG AGAAGAGCAC 
ATCCGAGTTT ATGCTATCGG GATCAAGGAG GCCAACCAAA CACAGCTGAG AGAAATTGCA 
GGAGAGGAAA AGAGAGTGTA TTACGTGCAT GACTTTGATG CATTGAAAGA CATAAGAAAC 
CAAGTTGTTC AAGAAATCTG TACTGAAGAA GCTTGCAAAG AGATGAAAGC TGACATCATG 
TTTCTGGTGG ACAGTTCTGG AAGTATAGGA CCTGAAAACT TCAGCAAAAT GAAAACATTT 
ATGAAAAACC TGGTGAGCAA GTCTCAGATT GGACCAGATC GGGTGCAAAT TGGTGTAGTC 
CAGTTCAGCG ACATCAATAA GGAAGAGTTT CAGCTCAACA GATTCATGTC CCAAAGCGAC 
ATTTCAAATG CAATAGACCA AATGGCTCAC ATTGGACAAA CCACCCTGAC TGGTAGTGCC 
CTGAGCTTTG TGTCTCAGTA CTTCAGCCCC ACCAAGGGCG CCCGGCCCAA CATCAGAAAG 
TTTCTCATCC TCATCACGGA TGGTGAAGCT CAGGACATAG TAAAGGAACC AGCAGTAGTG 
CTTCGGCAAG AAGGTGTAAT CATCTATTCT GTGGGAGTGT TTGGCTCCAA TGTCACCCAG 
CTTGAGGAGA TCAGTGGGAG GCCCGAGATG GTTTTTTATG TTGAGAATTT TGACATTCTG 
CAGCGCATTG AAGATGATCT TGTTTTTGGA ATATGCAGCC CCCGTGAAGA ATGCAAGCGG 
ATTGAAGTTT TAGACGTTGT GTTTGTCATT GATAGCTCTG GCAGTATTGA CTATGATGAG 
TATAATATCA TGAAGGATTT TATGATTGGC TTAGTGAAAA AAGCTGATGT GGGCAAGAAT 
CAGGTCCGGT TTGGGGCTCT GAAGTATGCT GATGACCCAG AGGTGCTGTT TTATCTGGAT 
GACTTTGGCA CAAAACTGGA GGTAATTTCA GTGCTCCAGA ATGACCAAGC CATGGGTGGC 
AGTACTTATA CTGCTGAGGC ACTGGGCTTC TCAGACCACA TGTTCACTGA AGCCCGGGGC 
AGCCGCCTGA ACAAGGGGGT CCCCCAAGTC CTCATTGTGA TCACCGATGG GGAATCCCAT 
GATGCTGATA AACTCAATGC CACGGCAAAG GCCTTGCGGG ACAAAGGCAT TCTTGTCCTG 
GCTGTGGGGA TTGATGGTGC CAATCCCGTG GAGCTGTTAG CCATGGCAGG ATCAAGCGAC 
AAGTACTTCT TCGTGGAGAC TTTTGGAGGT CTGAAGGGAA TATTTTCAGA TGTGACAGCC 
AGTGTCTGCA ACTCTTCAAA AGTAGATTGT GAAATTGACA AAGTAGATCT TGTTTTCCTT 
ATGGATGGTT CAACTAGCAT TCAGCCAAAT GACTTCAAGA AAATGAAGGA ATTTCTGGCA 
TCTGTTGTTC AAGACTTTGA TGTCAGCCTC AACAGAGTGC GAATAGGAGC GGCCCAGTTT 
AGCGATACCT ATCACCCGGA GTTTCCACTG GGAACTTTCA TAGGTGAAAA AGAGATATCA 
TTTCAGATTG AAAACATCAA GCAGATCTTT GGAAACACAC ACATCGGTGC TGCACTCAGG 
GAGGTGGAAC ATTACTTCAG GCCAGACATG GGCAGCAGGA TAAATACAGG TACCCCACAG 
GTGCTGCTGG TCCTTACAGA TGGCCAGTCC CAAGACGAGG TGGCCCAGGC CGCGGAAGCC 
CTGAGACACA GAGGTATCGA CATCTACTCC GTGGGCATTG GGGATGTGGA TGACCAGCAG 
CTCATTCAGA TCACCGGGAC TGCAGAGAAA AAACTGACAG TGCACAACTT CGATGAACTG 
AAGAAGGTCA ATAAAAGGAT CGTTCGCAAC ATCTGTACCA CAGCGGGTGA AAGCAACTGT 
TTCGTGGATG TTGTGGTGGG ATTTGATGTC TCAACTCAGG AGAAAGGGCA GACTTTGCTT 
GAAGGTCAGC CTTGGATGGA AACCTACCTT CAAGACATCT TACGTGCCAT CAGCTCCCTC 
AATGGAGTAA GCTGTGAGGT GGGCACAGAG ACTCAGGTCA GTGTGGCTTT TCAAGTGACC 
AATGCCATGG AAAAATATTC TCCCAAGTTT GAGATCTACA GTGAAAACAT ACTGAATAGC 
TTGAAGGATA TAACAGTTAA AGGACCATCT CTTCTCAATG CAAACCTCTT GGATTCTCTA 
TGGGATACAT TTCAGAATAA ATCAGCTGCT CGAGGAAAGG TGGTCCTTTT ATTTTCAGAT 
GGATTGGATG ATGATGTTGA GAAACTTGAA CAAAAATCTG ATGAACTTAG AAAAGAAGGC 
CTGAATGCCC TCATAACTGT TGCTCTGGAT GGACCTGCTG ATTCAAGTGA CTTGGCTGAT 
CTTCCCTATA TTGAATTTGG GAAAGGATTT GAGTACAGGA CACAGCTCTC TATTGGCATG 
AGAGAACTTG GAAGCCGGCT GTCAAAGCAG CTGGTCAATG TTGCTGAAAG GACATGCTGC 
TGTTTGTTCT GCAAGTGCAT TGGAGGAGAT GGCACAATGG GAGATCCTGG ACCACCAGGG 
AAAAGGGGAC CTCCAGGTTT TAAAGGCAGT GAAGGCTACC TGGGAGAGGA GGGAATCGCT 
GGAGAAAGAG GAGCCCCTGG ACCAGTGGGA GAGCAAGGTA CTAAGGGATG CTATGGCACC 
AAAGGTCCTA AGGGAAACAG AGGACTAAAT GGACAGGAGG GAGAAGTTGG GGAAAATGGA 
ATTGACGGAT TAAACGGAGA ACAGGGTGAT AATGGTCTTC CTGGAAGAAA AGGAGAAAAG 
GGAGATGAGG GATCTCAGGG AAGCCCAGGG AAGAGAGGGA CTCCTGGTGA CCGTGGAGCA 
AAGGGCCTGC GAGGGGATCC CGGAGCTCCT GGAGTTGACA GTAGCATAGA AGGACCCACA 
GGCTTGAAAG GAGAACGTGG AAGACAAGGC AGAAGAGGCT GGCCAGGCCC CCCCGGGACA 
CCAGGCTCCA GAAGAAAGAC AGCAGCTCAT GGCAGAAGGG GACATACAGG CCCACAGGGA 
ACAGCAGGCA TCCCAGGACC AGATGGACTT GAAGGCTCCC TGGGACTTAA GGGCCCTCAG 
GGCCCAAGAG GAGAGGCTGG TGTGAAAGGA GAAAAAGGAG GTGTGGGAAG TAAAGGTCCC 
CAGGGGCCTC CAGGACCCGG AGGAGAGGCA GGGAATCAAG GCCGTTTGGG AAGCCAAGGA 
AATAAAGGAG AACCTGGAGA TCTGGGAGAA AAAGGAGCTG TTGGCTTTCC TGGTCCTCGT 
GGCTTGCAGG GCAATGATGG CAGTCCAGGT TATGGTAGTG TCGGACGCAA GGGAGCAAAG 
GGACAAGAAG GATTCCCTGG AGAAAGTGGA CCTAAGGGTG AGATTGGGGA CCCTGGTGGT 
CCAGGAGAGA CTGGGCTGAA GGGAGCTAGA GGCAAAATGA TATCTGCTGG GCTTCCAGGA 
GAGATGGGAT CCCCTGGGGA ACCAGGACCT CCTGGACGTA AGGGTGTGAA AGGAGCCAAA 
GGCTTGGCTT CATTTTCTAC ATGTGAGCTC ATTCAGTATG TGCGAGACCG CAGTCCTGGC 
AGGCATGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_011512432.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510734.1 Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X15, mRNA.

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CloneID	OHu75656
Clone ID Related Accession (Same CDS sequence)	XM_011512432.2
Accession Version	XM_011512432.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512432.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTTT CCCCAAGATT TGAAGTTGAA GATTTTTCAG GTCATAATAT GATGTTGCTA 
ATTTTGTTCC TCGTGATAAT TTGTTCCCAT ATTTCTGTGA ACCAAGATTC CGGCCCTGAG 
TATGCAGATG TTGTGTTTTT GGTGGACAGC TCTGATCGCC TGGGATCCAA GTCCTTCCCA 
TTTGTGAAAA TGTTCATCAC CAAAATGATC AGCAGTCTCC CCATAGAGGC CGACAAATAC 
CGTGTGGCCC TGGCCCAGTA CAGTGATAAA CTTCACAGTG AATTCCACCT GAGCACCTTC 
AAAGGCAGGA GCCCCATGCT GAACCACCTA AGGAAGAACT TTGGATTCAT TGGCGGGTCC 
CTGCAGATAG GAAAGGCTCT TCAGGAGGCT CACAGGACTT ATTTCTCTGC ACCCGCAAAT 
GGGAGAGACA AGAAACAGTT TCCCCCAATT CTAGTGGTCC TGGCTTCATC TGAGTCTGAG 
GATAATGTGG AAGAGGCATC AAAGGCCCTG CGGAAAGACG GAGTGAAAAT CATCTCTGTA 
GGGGTGCAGA AAGCTTCTGA GGAAAACCTG AAGGCCATGG CCACGTCTCA GTTTCATTTC 
AACCTTCGGA CAGTCAGAGA CCTCAGCATG TTTTCCCAAA ACATGACACA CATCATCAAG 
GATGTAATAA AGTACAAGGA GGGAGCAGTT GATGACATCT TTGTAGAAGC TTGCCAAGGC 
CCTTCTATGG CCGATGTTGT GTTCCTATTG GATATGTCAA TCAATGGAAG TGAGGAGAAC 
TTTGACTATC TTAAAGGATT CTTGGAAGAA AGTGTATCTG CCCTTGACAT AAAGGAAAAT 
TGCATGAGGG TTGGCCTTGT GGCCTATAGC AATGAGACAA AAGTGATAAA TTCACTGAGC 
ATGGGCATAA ATAAGTCAGA GGTTCTCCAG CATATACAGA ACCTTTCTCC CCGAACTGGG 
AAGGCCTATA CTGGAGCTGC CATCAAAAAG CTCAGGAAGG AAGTTTTTAG TGCACGGAAT 
GGCAGTCGGA AGAATCAGGG GGTGCCCCAG ATTGCCGTGC TGGTGACCCA CCGAGATTCA 
GAAGACAACG TGACAAAAGC AGCTGTTAAC CTCCGACGGG AGGGTGTGAC CATCTTCACC 
CTGGGCATAG AGGGCGCCAG CGACACCCAG TTGGAAAAGA TAGCATCCCA CCCTGCTGAG 
CAGTATGTCT CCAAACTGAA GACCTTCGCT GACCTGGCTG CTCACAACCA GACATTTCTG 
AAGAAGCTGC GGAACCAAAT AACACACACA GTCTCTGTCT TTTCAGAGAG GACTGAAACG 
CTCAAATCTG GTTGTGTGGA CACTGAGGAA GCAGACATCT ATCTGCTTAT CGATGGCTCA 
GGGAGCACCC AGGCCACAGA TTTCCATGAA ATGAAGACGT TCCTGTCAGA GGTGGTAGGG 
ATGTTCAACA TTGCTCCCCA TAAGGTGCGG GTTGGGGCCG TTCAGTATGC TGACAGCTGG 
GACTTGGAAT TTGAGATCAA TAAATACTCC AACAAGCAGG ATTTGGGAAA GGCCATTGAG 
AATATCAGGC AGATGGGTGG GAATACAAAC ACAGGCGCAG CACTGAATTT CACACTGAGT 
CTGTTGCAAA AAGCAAAGAA GCAGCGAGGA AACAAAGTTC CATGCCACCT TGTTGTCCTG 
ACAAATGGCA TGTCCAAGGA TAGCATCTTG GAGCCTGCAA ACAGACTGAG AGAAGAGCAC 
ATCCGAGTTT ATGCTATCGG GATCAAGGAG GCCAACCAAA CACAGCTGAG AGAAATTGCA 
GGAGAGGAAA AGAGAGTGTA TTACGTGCAT GACTTTGATG CATTGAAAGA CATAAGAAAC 
CAAGTTGTTC AAGAAATCTG TACTGAAGAA GCTTGCAAAG AGATGAAAGC TGACATCATG 
TTTCTGGTGG ACAGTTCTGG AAGTATAGGA CCTGAAAACT TCAGCAAAAT GAAAACATTT 
ATGAAAAACC TGGTGAGCAA GTCTCAGATT GGACCAGATC GGGTGCAAAT TGGTGTAGTC 
CAGTTCAGCG ACATCAATAA GGAAGAGTTT CAGCTCAACA GATTCATGTC CCAAAGCGAC 
ATTTCAAATG CAATAGACCA AATGGCTCAC ATTGGACAAA CCACCCTGAC TGGTAGTGCC 
CTGAGCTTTG TGTCTCAGTA CTTCAGCCCC ACCAAGGGCG CCCGGCCCAA CATCAGAAAG 
TTTCTCATCC TCATCACGGA TGGTGAAGCT CAGGACATAG TAAAGGAACC AGCAGTAGTG 
CTTCGGCAAG AAGGTGTAAT CATCTATTCT GTGGGAGTGT TTGGCTCCAA TGTCACCCAG 
CTTGAGGAGA TCAGTGGGAG GCCCGAGATG GTTTTTTATG TTGAGAATTT TGACATTCTG 
CAGCGCATTG AAGATGATCT TGTTTTTGGA ATATGCAGCC CCCGTGAAGA ATGCAAGCGG 
ATTGAAGTTT TAGACGTTGT GTTTGTCATT GATAGCTCTG GCAGTATTGA CTATGATGAG 
TATAATATCA TGAAGGATTT TATGATTGGC TTAGTGAAAA AAGCTGATGT GGGCAAGAAT 
CAGGTCCGGT TTGGGGCTCT GAAGTATGCT GATGACCCAG AGGTGCTGTT TTATCTGGAT 
GACTTTGGCA CAAAACTGGA GGTAATTTCA GTGCTCCAGA ATGACCAAGC CATGGGTGGC 
AGTACTTATA CTGCTGAGGC ACTGGGCTTC TCAGACCACA TGTTCACTGA AGCCCGGGGC 
AGCCGCCTGA ACAAGGGGGT CCCCCAAGTC CTCATTGTGA TCACCGATGG GGAATCCCAT 
GATGCTGATA AACTCAATGC CACGGCAAAG GCCTTGCGGG ACAAAGGCAT TCTTGTCCTG 
GCTGTGGGGA TTGATGGTGC CAATCCCGTG GAGCTGTTAG CCATGGCAGG ATCAAGCGAC 
AAGTACTTCT TCGTGGAGAC TTTTGGAGGT CTGAAGGGAA TATTTTCAGA TGTGACAGCC 
AGTGTCTGCA ACTCTTCAAA AGTAGATTGT GAAATTGACA AAGTAGATCT TGTTTTCCTT 
ATGGATGGTT CAACTAGCAT TCAGCCAAAT GACTTCAAGA AAATGAAGGA ATTTCTGGCA 
TCTGTTGTTC AAGACTTTGA TGTCAGCCTC AACAGAGTGC GAATAGGAGC GGCCCAGTTT 
AGCGATACCT ATCACCCGGA GTTTCCACTG GGAACTTTCA TAGGTGAAAA AGAGATATCA 
TTTCAGATTG AAAACATCAA GCAGATCTTT GGAAACACAC ACATCGGTGC TGCACTCAGG 
GAGGTGGAAC ATTACTTCAG GCCAGACATG GGCAGCAGGA TAAATACAGG TACCCCACAG 
GTGCTGCTGG TCCTTACAGA TGGCCAGTCC CAAGACGAGG TGGCCCAGGC CGCGGAAGCC 
CTGAGACACA GAGGTATCGA CATCTACTCC GTGGGCATTG GGGATGTGGA TGACCAGCAG 
CTCATTCAGA TCACCGGGAC TGCAGAGAAA AAACTGACAG TGCACAACTT CGATGAACTG 
AAGAAGGTCA ATAAAAGGAT CGTTCGCAAC ATCTGTACCA CAGCGGGTGA AAGCAACTGT 
TTCGTGGATG TTGTGGTGGG ATTTGATGTC TCAACTCAGG AGAAAGGGCA GACTTTGCTT 
GAAGGTCAGC CTTGGATGGA AACCTACCTT CAAGACATCT TACGTGCCAT CAGCTCCCTC 
AATGGAGTAA GCTGTGAGGT GGGCACAGAG ACTCAGGTCA GTGTGGCTTT TCAAGTGACC 
AATGCCATGG AAAAATATTC TCCCAAGTTT GAGATCTACA GTGAAAACAT ACTGAATAGC 
TTGAAGGATA TAACAGTTAA AGGACCATCT CTTCTCAATG CAAACCTCTT GGATTCTCTA 
TGGGATACAT TTCAGAATAA ATCAGCTGCT CGAGGAAAGG TGGTCCTTTT ATTTTCAGAT 
GGATTGGATG ATGATGTTGA GAAACTTGAA CAAAAATCTG ATGAACTTAG AAAAGAAGGC 
CTGAATGCCC TCATAACTGT TGCTCTGGAT GGACCTGCTG ATTCAAGTGA CTTGGCTGAT 
CTTCCCTATA TTGAATTTGG GAAAGGATTT GAGTACAGGA CACAGCTCTC TATTGGCATG 
AGAGAACTTG GAAGCCGGCT GTCAAAGCAG CTGGTCAATG TTGCTGAAAG GACATGCTGC 
TGTTTGTTCT GCAAGTGCAT TGGAGGAGAT GGCACAATGG GAGATCCTGG ACCACCAGGG 
AAAAGGGGAC CTCCAGGTTT TAAAGGCAGT GAAGGCTACC TGGGAGAGGA GGGAATCGCT 
GGAGAAAGAG GAGCCCCTGG ACCAGTGGGA GAGCAAGGTA CTAAGGGATG CTATGGCACC 
AAAGGTCCTA AGGGAAACAG AGGACTAAAT GGACAGGAGG GAGAAGTTGG GGAAAATGGA 
ATTGACGGAT TAAACGGAGA ACAGGGTGAT AATGGTCTTC CTGGAAGAAA AGGAGAAAAG 
GGAGATGAGG GATCTCAGGG AAGCCCAGGG AAGAGAGGGA CTCCTGGTGA CCGTGGAGCA 
AAGGGCCTGC GAGGGGATCC CGGAGCTCCT GGAGTTGACA GTAGCATAGA AGGACCCACA 
GGCTTGAAAG GAGAACGTGG AAGACAAGGC AGAAGAGGCT GGCCAGGCCC CCCCGGGACA 
CCAGGCTCCA GAAGAAAGAC AGCAGCTCAT GGCAGAAGGG GACATACAGG CCCACAGGGA 
ACAGCAGGCA TCCCAGGACC AGATGGACTT GAAGGCTCCC TGGGACTTAA GGGCCCTCAG 
GGCCCAAGAG GAGAGGCTGG TGTGAAAGGA GAAAAAGGAG GTGTGGGAAG TAAAGGTCCC 
CAGGGGCCTC CAGGACCCGG AGGAGAGGCA GGGAATCAAG GCCGTTTGGG AAGCCAAGGA 
AATAAAGGAG AACCTGGAGA TCTGGGAGAA AAAGGAGCTG TTGGCTTTCC TGGTCCTCGT 
GGCTTGCAGG GCAATGATGG CAGTCCAGGT TATGGTAGTG TCGGACGCAA GGGAGCAAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510734.1
CDS	209..5251
Translation	MEVSPRFEVEDFSGHNMMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAK

Target ORF information:

RefSeq Version	XM_011512432.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512432.2

ORF Insert Sequence:

ATGGAAGTTT CCCCAAGATT TGAAGTTGAA GATTTTTCAG GTCATAATAT GATGTTGCTA 
ATTTTGTTCC TCGTGATAAT TTGTTCCCAT ATTTCTGTGA ACCAAGATTC CGGCCCTGAG 
TATGCAGATG TTGTGTTTTT GGTGGACAGC TCTGATCGCC TGGGATCCAA GTCCTTCCCA 
TTTGTGAAAA TGTTCATCAC CAAAATGATC AGCAGTCTCC CCATAGAGGC CGACAAATAC 
CGTGTGGCCC TGGCCCAGTA CAGTGATAAA CTTCACAGTG AATTCCACCT GAGCACCTTC 
AAAGGCAGGA GCCCCATGCT GAACCACCTA AGGAAGAACT TTGGATTCAT TGGCGGGTCC 
CTGCAGATAG GAAAGGCTCT TCAGGAGGCT CACAGGACTT ATTTCTCTGC ACCCGCAAAT 
GGGAGAGACA AGAAACAGTT TCCCCCAATT CTAGTGGTCC TGGCTTCATC TGAGTCTGAG 
GATAATGTGG AAGAGGCATC AAAGGCCCTG CGGAAAGACG GAGTGAAAAT CATCTCTGTA 
GGGGTGCAGA AAGCTTCTGA GGAAAACCTG AAGGCCATGG CCACGTCTCA GTTTCATTTC 
AACCTTCGGA CAGTCAGAGA CCTCAGCATG TTTTCCCAAA ACATGACACA CATCATCAAG 
GATGTAATAA AGTACAAGGA GGGAGCAGTT GATGACATCT TTGTAGAAGC TTGCCAAGGC 
CCTTCTATGG CCGATGTTGT GTTCCTATTG GATATGTCAA TCAATGGAAG TGAGGAGAAC 
TTTGACTATC TTAAAGGATT CTTGGAAGAA AGTGTATCTG CCCTTGACAT AAAGGAAAAT 
TGCATGAGGG TTGGCCTTGT GGCCTATAGC AATGAGACAA AAGTGATAAA TTCACTGAGC 
ATGGGCATAA ATAAGTCAGA GGTTCTCCAG CATATACAGA ACCTTTCTCC CCGAACTGGG 
AAGGCCTATA CTGGAGCTGC CATCAAAAAG CTCAGGAAGG AAGTTTTTAG TGCACGGAAT 
GGCAGTCGGA AGAATCAGGG GGTGCCCCAG ATTGCCGTGC TGGTGACCCA CCGAGATTCA 
GAAGACAACG TGACAAAAGC AGCTGTTAAC CTCCGACGGG AGGGTGTGAC CATCTTCACC 
CTGGGCATAG AGGGCGCCAG CGACACCCAG TTGGAAAAGA TAGCATCCCA CCCTGCTGAG 
CAGTATGTCT CCAAACTGAA GACCTTCGCT GACCTGGCTG CTCACAACCA GACATTTCTG 
AAGAAGCTGC GGAACCAAAT AACACACACA GTCTCTGTCT TTTCAGAGAG GACTGAAACG 
CTCAAATCTG GTTGTGTGGA CACTGAGGAA GCAGACATCT ATCTGCTTAT CGATGGCTCA 
GGGAGCACCC AGGCCACAGA TTTCCATGAA ATGAAGACGT TCCTGTCAGA GGTGGTAGGG 
ATGTTCAACA TTGCTCCCCA TAAGGTGCGG GTTGGGGCCG TTCAGTATGC TGACAGCTGG 
GACTTGGAAT TTGAGATCAA TAAATACTCC AACAAGCAGG ATTTGGGAAA GGCCATTGAG 
AATATCAGGC AGATGGGTGG GAATACAAAC ACAGGCGCAG CACTGAATTT CACACTGAGT 
CTGTTGCAAA AAGCAAAGAA GCAGCGAGGA AACAAAGTTC CATGCCACCT TGTTGTCCTG 
ACAAATGGCA TGTCCAAGGA TAGCATCTTG GAGCCTGCAA ACAGACTGAG AGAAGAGCAC 
ATCCGAGTTT ATGCTATCGG GATCAAGGAG GCCAACCAAA CACAGCTGAG AGAAATTGCA 
GGAGAGGAAA AGAGAGTGTA TTACGTGCAT GACTTTGATG CATTGAAAGA CATAAGAAAC 
CAAGTTGTTC AAGAAATCTG TACTGAAGAA GCTTGCAAAG AGATGAAAGC TGACATCATG 
TTTCTGGTGG ACAGTTCTGG AAGTATAGGA CCTGAAAACT TCAGCAAAAT GAAAACATTT 
ATGAAAAACC TGGTGAGCAA GTCTCAGATT GGACCAGATC GGGTGCAAAT TGGTGTAGTC 
CAGTTCAGCG ACATCAATAA GGAAGAGTTT CAGCTCAACA GATTCATGTC CCAAAGCGAC 
ATTTCAAATG CAATAGACCA AATGGCTCAC ATTGGACAAA CCACCCTGAC TGGTAGTGCC 
CTGAGCTTTG TGTCTCAGTA CTTCAGCCCC ACCAAGGGCG CCCGGCCCAA CATCAGAAAG 
TTTCTCATCC TCATCACGGA TGGTGAAGCT CAGGACATAG TAAAGGAACC AGCAGTAGTG 
CTTCGGCAAG AAGGTGTAAT CATCTATTCT GTGGGAGTGT TTGGCTCCAA TGTCACCCAG 
CTTGAGGAGA TCAGTGGGAG GCCCGAGATG GTTTTTTATG TTGAGAATTT TGACATTCTG 
CAGCGCATTG AAGATGATCT TGTTTTTGGA ATATGCAGCC CCCGTGAAGA ATGCAAGCGG 
ATTGAAGTTT TAGACGTTGT GTTTGTCATT GATAGCTCTG GCAGTATTGA CTATGATGAG 
TATAATATCA TGAAGGATTT TATGATTGGC TTAGTGAAAA AAGCTGATGT GGGCAAGAAT 
CAGGTCCGGT TTGGGGCTCT GAAGTATGCT GATGACCCAG AGGTGCTGTT TTATCTGGAT 
GACTTTGGCA CAAAACTGGA GGTAATTTCA GTGCTCCAGA ATGACCAAGC CATGGGTGGC 
AGTACTTATA CTGCTGAGGC ACTGGGCTTC TCAGACCACA TGTTCACTGA AGCCCGGGGC 
AGCCGCCTGA ACAAGGGGGT CCCCCAAGTC CTCATTGTGA TCACCGATGG GGAATCCCAT 
GATGCTGATA AACTCAATGC CACGGCAAAG GCCTTGCGGG ACAAAGGCAT TCTTGTCCTG 
GCTGTGGGGA TTGATGGTGC CAATCCCGTG GAGCTGTTAG CCATGGCAGG ATCAAGCGAC 
AAGTACTTCT TCGTGGAGAC TTTTGGAGGT CTGAAGGGAA TATTTTCAGA TGTGACAGCC 
AGTGTCTGCA ACTCTTCAAA AGTAGATTGT GAAATTGACA AAGTAGATCT TGTTTTCCTT 
ATGGATGGTT CAACTAGCAT TCAGCCAAAT GACTTCAAGA AAATGAAGGA ATTTCTGGCA 
TCTGTTGTTC AAGACTTTGA TGTCAGCCTC AACAGAGTGC GAATAGGAGC GGCCCAGTTT 
AGCGATACCT ATCACCCGGA GTTTCCACTG GGAACTTTCA TAGGTGAAAA AGAGATATCA 
TTTCAGATTG AAAACATCAA GCAGATCTTT GGAAACACAC ACATCGGTGC TGCACTCAGG 
GAGGTGGAAC ATTACTTCAG GCCAGACATG GGCAGCAGGA TAAATACAGG TACCCCACAG 
GTGCTGCTGG TCCTTACAGA TGGCCAGTCC CAAGACGAGG TGGCCCAGGC CGCGGAAGCC 
CTGAGACACA GAGGTATCGA CATCTACTCC GTGGGCATTG GGGATGTGGA TGACCAGCAG 
CTCATTCAGA TCACCGGGAC TGCAGAGAAA AAACTGACAG TGCACAACTT CGATGAACTG 
AAGAAGGTCA ATAAAAGGAT CGTTCGCAAC ATCTGTACCA CAGCGGGTGA AAGCAACTGT 
TTCGTGGATG TTGTGGTGGG ATTTGATGTC TCAACTCAGG AGAAAGGGCA GACTTTGCTT 
GAAGGTCAGC CTTGGATGGA AACCTACCTT CAAGACATCT TACGTGCCAT CAGCTCCCTC 
AATGGAGTAA GCTGTGAGGT GGGCACAGAG ACTCAGGTCA GTGTGGCTTT TCAAGTGACC 
AATGCCATGG AAAAATATTC TCCCAAGTTT GAGATCTACA GTGAAAACAT ACTGAATAGC 
TTGAAGGATA TAACAGTTAA AGGACCATCT CTTCTCAATG CAAACCTCTT GGATTCTCTA 
TGGGATACAT TTCAGAATAA ATCAGCTGCT CGAGGAAAGG TGGTCCTTTT ATTTTCAGAT 
GGATTGGATG ATGATGTTGA GAAACTTGAA CAAAAATCTG ATGAACTTAG AAAAGAAGGC 
CTGAATGCCC TCATAACTGT TGCTCTGGAT GGACCTGCTG ATTCAAGTGA CTTGGCTGAT 
CTTCCCTATA TTGAATTTGG GAAAGGATTT GAGTACAGGA CACAGCTCTC TATTGGCATG 
AGAGAACTTG GAAGCCGGCT GTCAAAGCAG CTGGTCAATG TTGCTGAAAG GACATGCTGC 
TGTTTGTTCT GCAAGTGCAT TGGAGGAGAT GGCACAATGG GAGATCCTGG ACCACCAGGG 
AAAAGGGGAC CTCCAGGTTT TAAAGGCAGT GAAGGCTACC TGGGAGAGGA GGGAATCGCT 
GGAGAAAGAG GAGCCCCTGG ACCAGTGGGA GAGCAAGGTA CTAAGGGATG CTATGGCACC 
AAAGGTCCTA AGGGAAACAG AGGACTAAAT GGACAGGAGG GAGAAGTTGG GGAAAATGGA 
ATTGACGGAT TAAACGGAGA ACAGGGTGAT AATGGTCTTC CTGGAAGAAA AGGAGAAAAG 
GGAGATGAGG GATCTCAGGG AAGCCCAGGG AAGAGAGGGA CTCCTGGTGA CCGTGGAGCA 
AAGGGCCTGC GAGGGGATCC CGGAGCTCCT GGAGTTGACA GTAGCATAGA AGGACCCACA 
GGCTTGAAAG GAGAACGTGG AAGACAAGGC AGAAGAGGCT GGCCAGGCCC CCCCGGGACA 
CCAGGCTCCA GAAGAAAGAC AGCAGCTCAT GGCAGAAGGG GACATACAGG CCCACAGGGA 
ACAGCAGGCA TCCCAGGACC AGATGGACTT GAAGGCTCCC TGGGACTTAA GGGCCCTCAG 
GGCCCAAGAG GAGAGGCTGG TGTGAAAGGA GAAAAAGGAG GTGTGGGAAG TAAAGGTCCC 
CAGGGGCCTC CAGGACCCGG AGGAGAGGCA GGGAATCAAG GCCGTTTGGG AAGCCAAGGA 
AATAAAGGAG AACCTGGAGA TCTGGGAGAA AAAGGAGCTG TTGGCTTTCC TGGTCCTCGT 
GGCTTGCAGG GCAATGATGG CAGTCCAGGT TATGGTAGTG TCGGACGCAA GGGAGCAAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( NM_001102608.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001096078.1 Homo sapiens collagen type VI alpha 6 chain (COL6A6), mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	NM_001102608.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-21
Organism	Homo sapiens(Human)
Product	collagen alpha-6(VI) chain precursor
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AM774225.1, AM774226.1, AC093006.4, AM774227.1 and AL713792.1. On or before Aug 31, 2007 this sequence version replaced XM_067585.12, XM_940071.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2142363, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096078.1
CDS	32..6823
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	NM_001102608.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens collagen type VI alpha 6 chain (COL6A6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102608.1

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( NM_001102608.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001096078.1 Homo sapiens collagen type VI alpha 6 chain (COL6A6), mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	NM_001102608.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AM774225.1, AM774226.1, AC093006.4, AM774227.1, AL713792.1, AC128683.3, AA455864.1, AI761416.1 and AF086237.1. This sequence is a reference standard in the RefSeqGene project. On May 24, 2017 this sequence version replaced NM_001102608.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2142363, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096078.1
CDS	32..6823
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	NM_001102608.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 chain (COL6A6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102608.2

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_017005711.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861200.1 Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X4, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_017005711.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861200.1
CDS	476..7267
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_017005711.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005711.1

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_017005712.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861201.1 Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X5, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_017005712.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005712.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861201.1
CDS	574..7365
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_017005712.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005712.2

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_011512425.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510727.1 Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X7, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_011512425.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512425.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510727.1
CDS	371..7162
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_011512425.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512425.3

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_017005715.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861204.1 Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X11, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_017005715.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005715.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861204.1
CDS	397..7188
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_017005715.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005715.2

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_011512425.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510727.1 Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X7, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_011512425.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510727.1
CDS	371..7162
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_011512425.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512425.2

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_017005715.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861204.1 Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X11, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_017005715.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861204.1
CDS	397..7188
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_017005715.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005715.1

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_005247121.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005247178.1 Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X9, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_005247121.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005247121.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005247178.1
CDS	191..6982
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_005247121.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005247121.4

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_017005713.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861202.1 Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X6, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_017005713.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861202.1
CDS	527..7318
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_017005713.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005713.1

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_011512426.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510728.1 Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X9, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_011512426.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512426.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510728.1
CDS	265..7056
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_011512426.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512426.3

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_011512428.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510730.1 Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X12, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_011512428.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512428.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510730.1
CDS	292..7083
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_011512428.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512428.3

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_017005712.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861201.1 Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X5, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_017005712.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861201.1
CDS	580..7371
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_017005712.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005712.1

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_011512428.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510730.1 Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X12, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_011512428.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510730.1
CDS	292..7083
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_011512428.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512428.2

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_017005713.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861202.1 Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X6, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_017005713.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005713.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861202.1
CDS	527..7318
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_017005713.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005713.2

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_017005714.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861203.1 Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X8, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_017005714.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005714.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861203.1
CDS	6632..13423
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_017005714.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005714.2

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_005247121.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005247178.1 Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X10, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_005247121.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005247121.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005247178.1
CDS	149..6940
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_005247121.5
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005247121.5

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_011512426.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510728.1 Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X10, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_011512426.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510728.1
CDS	265..7056
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_011512426.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512426.2

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_017005714.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861203.1 Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X8, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_017005714.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861203.1
CDS	6632..13423
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_017005714.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005714.1

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_017005711.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861200.1 Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X4, mRNA.

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CloneID	OHu06178
Clone ID Related Accession (Same CDS sequence)	NM_001102608.1 , XM_017005711.1 , NM_001102608.2 , XM_017005712.2 , XM_011512425.3 , XM_017005715.2 , XM_011512425.2 , XM_017005715.1 , XM_005247121.4 , XM_017005713.1 , XM_011512426.3 , XM_011512428.3 , XM_017005712.1 , XM_011512428.2 , XM_017005713.2 , XM_017005714.2 , XM_005247121.5 , XM_011512426.2 , XM_017005714.1 , XM_017005711.2
Accession Version	XM_017005711.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005711.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861200.1
CDS	476..7267
Translation	MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_017005711.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005711.2

ORF Insert Sequence:

ATGATGTTGC TAATTTTGTT CCTCGTGATA ATTTGTTCCC ATATTTCTGT GAACCAAGAT 
TCCGGCCCTG AGTATGCAGA TGTTGTGTTT TTGGTGGACA GCTCTGATCG CCTGGGATCC 
AAGTCCTTCC CATTTGTGAA AATGTTCATC ACCAAAATGA TCAGCAGTCT CCCCATAGAG 
GCCGACAAAT ACCGTGTGGC CCTGGCCCAG TACAGTGATA AACTTCACAG TGAATTCCAC 
CTGAGCACCT TCAAAGGCAG GAGCCCCATG CTGAACCACC TAAGGAAGAA CTTTGGATTC 
ATTGGCGGGT CCCTGCAGAT AGGAAAGGCT CTTCAGGAGG CTCACAGGAC TTATTTCTCT 
GCACCCGCAA ATGGGAGAGA CAAGAAACAG TTTCCCCCAA TTCTAGTGGT CCTGGCTTCA 
TCTGAGTCTG AGGATAATGT GGAAGAGGCA TCAAAGGCCC TGCGGAAAGA CGGAGTGAAA 
ATCATCTCTG TAGGGGTGCA GAAAGCTTCT GAGGAAAACC TGAAGGCCAT GGCCACGTCT 
CAGTTTCATT TCAACCTTCG GACAGTCAGA GACCTCAGCA TGTTTTCCCA AAACATGACA 
CACATCATCA AGGATGTAAT AAAGTACAAG GAGGGAGCAG TTGATGACAT CTTTGTAGAA 
GCTTGCCAAG GCCCTTCTAT GGCCGATGTT GTGTTCCTAT TGGATATGTC AATCAATGGA 
AGTGAGGAGA ACTTTGACTA TCTTAAAGGA TTCTTGGAAG AAAGTGTATC TGCCCTTGAC 
ATAAAGGAAA ATTGCATGAG GGTTGGCCTT GTGGCCTATA GCAATGAGAC AAAAGTGATA 
AATTCACTGA GCATGGGCAT AAATAAGTCA GAGGTTCTCC AGCATATACA GAACCTTTCT 
CCCCGAACTG GGAAGGCCTA TACTGGAGCT GCCATCAAAA AGCTCAGGAA GGAAGTTTTT 
AGTGCACGGA ATGGCAGTCG GAAGAATCAG GGGGTGCCCC AGATTGCCGT GCTGGTGACC 
CACCGAGATT CAGAAGACAA CGTGACAAAA GCAGCTGTTA ACCTCCGACG GGAGGGTGTG 
ACCATCTTCA CCCTGGGCAT AGAGGGCGCC AGCGACACCC AGTTGGAAAA GATAGCATCC 
CACCCTGCTG AGCAGTATGT CTCCAAACTG AAGACCTTCG CTGACCTGGC TGCTCACAAC 
CAGACATTTC TGAAGAAGCT GCGGAACCAA ATAACACACA CAGTCTCTGT CTTTTCAGAG 
AGGACTGAAA CGCTCAAATC TGGTTGTGTG GACACTGAGG AAGCAGACAT CTATCTGCTT 
ATCGATGGCT CAGGGAGCAC CCAGGCCACA GATTTCCATG AAATGAAGAC GTTCCTGTCA 
GAGGTGGTAG GGATGTTCAA CATTGCTCCC CATAAGGTGC GGGTTGGGGC CGTTCAGTAT 
GCTGACAGCT GGGACTTGGA ATTTGAGATC AATAAATACT CCAACAAGCA GGATTTGGGA 
AAGGCCATTG AGAATATCAG GCAGATGGGT GGGAATACAA ACACAGGCGC AGCACTGAAT 
TTCACACTGA GTCTGTTGCA AAAAGCAAAG AAGCAGCGAG GAAACAAAGT TCCATGCCAC 
CTTGTTGTCC TGACAAATGG CATGTCCAAG GATAGCATCT TGGAGCCTGC AAACAGACTG 
AGAGAAGAGC ACATCCGAGT TTATGCTATC GGGATCAAGG AGGCCAACCA AACACAGCTG 
AGAGAAATTG CAGGAGAGGA AAAGAGAGTG TATTACGTGC ATGACTTTGA TGCATTGAAA 
GACATAAGAA ACCAAGTTGT TCAAGAAATC TGTACTGAAG AAGCTTGCAA AGAGATGAAA 
GCTGACATCA TGTTTCTGGT GGACAGTTCT GGAAGTATAG GACCTGAAAA CTTCAGCAAA 
ATGAAAACAT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGACCAGA TCGGGTGCAA 
ATTGGTGTAG TCCAGTTCAG CGACATCAAT AAGGAAGAGT TTCAGCTCAA CAGATTCATG 
TCCCAAAGCG ACATTTCAAA TGCAATAGAC CAAATGGCTC ACATTGGACA AACCACCCTG 
ACTGGTAGTG CCCTGAGCTT TGTGTCTCAG TACTTCAGCC CCACCAAGGG CGCCCGGCCC 
AACATCAGAA AGTTTCTCAT CCTCATCACG GATGGTGAAG CTCAGGACAT AGTAAAGGAA 
CCAGCAGTAG TGCTTCGGCA AGAAGGTGTA ATCATCTATT CTGTGGGAGT GTTTGGCTCC 
AATGTCACCC AGCTTGAGGA GATCAGTGGG AGGCCCGAGA TGGTTTTTTA TGTTGAGAAT 
TTTGACATTC TGCAGCGCAT TGAAGATGAT CTTGTTTTTG GAATATGCAG CCCCCGTGAA 
GAATGCAAGC GGATTGAAGT TTTAGACGTT GTGTTTGTCA TTGATAGCTC TGGCAGTATT 
GACTATGATG AGTATAATAT CATGAAGGAT TTTATGATTG GCTTAGTGAA AAAAGCTGAT 
GTGGGCAAGA ATCAGGTCCG GTTTGGGGCT CTGAAGTATG CTGATGACCC AGAGGTGCTG 
TTTTATCTGG ATGACTTTGG CACAAAACTG GAGGTAATTT CAGTGCTCCA GAATGACCAA 
GCCATGGGTG GCAGTACTTA TACTGCTGAG GCACTGGGCT TCTCAGACCA CATGTTCACT 
GAAGCCCGGG GCAGCCGCCT GAACAAGGGG GTCCCCCAAG TCCTCATTGT GATCACCGAT 
GGGGAATCCC ATGATGCTGA TAAACTCAAT GCCACGGCAA AGGCCTTGCG GGACAAAGGC 
ATTCTTGTCC TGGCTGTGGG GATTGATGGT GCCAATCCCG TGGAGCTGTT AGCCATGGCA 
GGATCAAGCG ACAAGTACTT CTTCGTGGAG ACTTTTGGAG GTCTGAAGGG AATATTTTCA 
GATGTGACAG CCAGTGTCTG CAACTCTTCA AAAGTAGATT GTGAAATTGA CAAAGTAGAT 
CTTGTTTTCC TTATGGATGG TTCAACTAGC ATTCAGCCAA ATGACTTCAA GAAAATGAAG 
GAATTTCTGG CATCTGTTGT TCAAGACTTT GATGTCAGCC TCAACAGAGT GCGAATAGGA 
GCGGCCCAGT TTAGCGATAC CTATCACCCG GAGTTTCCAC TGGGAACTTT CATAGGTGAA 
AAAGAGATAT CATTTCAGAT TGAAAACATC AAGCAGATCT TTGGAAACAC ACACATCGGT 
GCTGCACTCA GGGAGGTGGA ACATTACTTC AGGCCAGACA TGGGCAGCAG GATAAATACA 
GGTACCCCAC AGGTGCTGCT GGTCCTTACA GATGGCCAGT CCCAAGACGA GGTGGCCCAG 
GCCGCGGAAG CCCTGAGACA CAGAGGTATC GACATCTACT CCGTGGGCAT TGGGGATGTG 
GATGACCAGC AGCTCATTCA GATCACCGGG ACTGCAGAGA AAAAACTGAC AGTGCACAAC 
TTCGATGAAC TGAAGAAGGT CAATAAAAGG ATCGTTCGCA ACATCTGTAC CACAGCGGGT 
GAAAGCAACT GTTTCGTGGA TGTTGTGGTG GGATTTGATG TCTCAACTCA GGAGAAAGGG 
CAGACTTTGC TTGAAGGTCA GCCTTGGATG GAAACCTACC TTCAAGACAT CTTACGTGCC 
ATCAGCTCCC TCAATGGAGT AAGCTGTGAG GTGGGCACAG AGACTCAGGT CAGTGTGGCT 
TTTCAAGTGA CCAATGCCAT GGAAAAATAT TCTCCCAAGT TTGAGATCTA CAGTGAAAAC 
ATACTGAATA GCTTGAAGGA TATAACAGTT AAAGGACCAT CTCTTCTCAA TGCAAACCTC 
TTGGATTCTC TATGGGATAC ATTTCAGAAT AAATCAGCTG CTCGAGGAAA GGTGGTCCTT 
TTATTTTCAG ATGGATTGGA TGATGATGTT GAGAAACTTG AACAAAAATC TGATGAACTT 
AGAAAAGAAG GCCTGAATGC CCTCATAACT GTTGCTCTGG ATGGACCTGC TGATTCAAGT 
GACTTGGCTG ATCTTCCCTA TATTGAATTT GGGAAAGGAT TTGAGTACAG GACACAGCTC 
TCTATTGGCA TGAGAGAACT TGGAAGCCGG CTGTCAAAGC AGCTGGTCAA TGTTGCTGAA 
AGGACATGCT GCTGTTTGTT CTGCAAGTGC ATTGGAGGAG ATGGCACAAT GGGAGATCCT 
GGACCACCAG GGAAAAGGGG ACCTCCAGGT TTTAAAGGCA GTGAAGGCTA CCTGGGAGAG 
GAGGGAATCG CTGGAGAAAG AGGAGCCCCT GGACCAGTGG GAGAGCAAGG TACTAAGGGA 
TGCTATGGCA CCAAAGGTCC TAAGGGAAAC AGAGGACTAA ATGGACAGGA GGGAGAAGTT 
GGGGAAAATG GAATTGACGG ATTAAACGGA GAACAGGGTG ATAATGGTCT TCCTGGAAGA 
AAAGGAGAAA AGGGAGATGA GGGATCTCAG GGAAGCCCAG GGAAGAGAGG GACTCCTGGT 
GACCGTGGAG CAAAGGGCCT GCGAGGGGAT CCCGGAGCTC CTGGAGTTGA CAGTAGCATA 
GAAGGACCCA CAGGCTTGAA AGGAGAACGT GGAAGACAAG GCAGAAGAGG CTGGCCAGGC 
CCCCCCGGGA CACCAGGCTC CAGAAGAAAG ACAGCAGCTC ATGGCAGAAG GGGACATACA 
GGCCCACAGG GAACAGCAGG CATCCCAGGA CCAGATGGAC TTGAAGGCTC CCTGGGACTT 
AAGGGCCCTC AGGGCCCAAG AGGAGAGGCT GGTGTGAAAG GAGAAAAAGG AGGTGTGGGA 
AGTAAAGGTC CCCAGGGGCC TCCAGGACCC GGAGGAGAGG CAGGGAATCA AGGCCGTTTG 
GGAAGCCAAG GAAATAAAGG AGAACCTGGA GATCTGGGAG AAAAAGGAGC TGTTGGCTTT 
CCTGGTCCTC GTGGCTTGCA GGGCAATGAT GGCAGTCCAG GTTATGGTAG TGTCGGACGC 
AAGGGAGCAA AGGGACAAGA AGGATTCCCT GGAGAAAGTG GACCTAAGGG TGAGATTGGG 
GACCCTGGTG GTCCAGGAGA GACTGGGCTG AAGGGAGCTA GAGGCAAAAT GATATCTGCT 
GGGCTTCCAG GAGAGATGGG ATCCCCTGGG GAACCAGGAC CTCCTGGACG TAAGGGTGTG 
AAAGGAGCCA AAGGCTTGGC TTCATTTTCT ACATGTGAGC TCATTCAGTA TGTGCGAGAC 
CGCAGTCCTG GCAGGCATGG AAAACCGGAA TGCCCAGTGC ACCCAACCGA GTTGGTGTTT 
GCCCTGGACC ACTCCCGGGA TGTCACTGAG CAGGAATTTG AGCGGATGAA GGAGATGATG 
GCTTTCCTGG TGAGAGACAT TAAGGTCCGG GAGAACAGCT GCCCCGTGGG AGCGCACATC 
GCCATCCTCT CCTATAACTC CCACGCCAGG CACCTTGTGC GCTTCTCAGA CGCCTACAAG 
AAGAGTCAAC TTCTCAGAGA AATTGAAACT ATTCCTTATG AGAGATCCTC TGCCAGCAGG 
GAGATTGGCA GAGCAATGCG GTTTATTTCC AGGAATGTCT TCAAGCGGAC GCTTCCGGGG 
GCACACACGA GAAAAATCGC CACATTTTTC AGCAGCGGTC AGTCCGCGGA TGCCCACTCC 
ATCACCACGG CTGCCATGGA GTTCGGCGCG CTTGAAATCA TTCCCGTGGT GATCACTTTC 
AGCAACGTGC CCTCGGTCAG GCGCGCATTT GCGATTGACG ACACTGGCAC ATTTCAAGTA 
ATAGTGGTTC CCTCCGGGGC CGACTACATA CCAGCATTAG AGAGACTCCA GCGGTGCACT 
TTCTGCTATG ATGTGTGCAA GCCAGATGCT TCTTGTGACC AAGCCAGACC ACCCCCTGTG 
CAGTCTTACA TGGATGCTGC TTTCCTTCTG GATGCCTCCC GGAACATGGG AAGTGCTGAA 
TTTGAAGACA TAAGAGCCTT CCTTGGAGCA CTATTAGATC ACTTTGAAAT CACCCCAGAG 
CCGGAGACTT CTGTCACTGG AGACCGGGTG GCCCTATTGA GCCATGCTCC CCCCGACTTC 
CTACCCAACA CTCAGAAGAG TCCAGTTAGA GCTGAGTTCA ATCTTACCAC CTACAGAAGT 
AAGCGCCTCA TGAAGAGGCA TGTGCACGAG TCAGTTAAAC AACTAAATGG AGATGCTTTT 
ATTGGTCATG CCTTACAGTG GACTCTGGAC AATGTATTTT TAAGTACACC CAATCTGAGA 
AGAAACAAAG TCATATTTGT GATATCTGCT GGGGAAACCA GCCACTTAGA TGGGGAAATC 
TTAAAGAAGG AATCCTTGCG AGCCAAATGT CAGGGATATG CCTTATTTGT GTTTTCCCTT 
GGCCCTATTT GGGATGACAA GGAACTGGAG GATCTCGCCA GCCACCCTTT GGATCACCAC 
CTGGTCCAGC TTGGCCGAAT TCATAAACCT GACCACAGTT ATGGTGTGAA GTTTGTGAAG 
TCCTTTATAA ACTCAATCAG GCGTGCAATC AACAAATATC CACCAATAAA CTTAAAAATA 
AAGTGCAACA GACTTAACTC TATAGATCCA AAGCAGCCCC CACGACCATT CCGAAGCTTT 
GTTCCTGGAC CACTTAAAGC TACCCTCAAA GAAGATGTAT TACAGAAGGC AAAATTCTTT 
CAAGATAAAA AATATCTTTC AAGAGTAGCA AGAAGTGGCA GAGATGATGC TATTCAAAAT 
TTTATGAGAA GCACCTCCCA TACCTTTAAG AATGGAAGGA TGATAGAAAG TGCTCCCAAA 
CAACATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_011512423.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510725.1 Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X1, mRNA.

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CloneID	OHu75652
Clone ID Related Accession (Same CDS sequence)	XM_011512423.3 , XM_011512424.2 , XM_011512423.2
Accession Version	XM_011512423.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512423.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTTT CCCCAAGATT TGAAGTTGAA GATTTTTCAG GTCATAATAT GATGTTGCTA 
ATTTTGTTCC TCGTGATAAT TTGTTCCCAT ATTTCTGTGA ACCAAGATTC CGGCCCTGAG 
TATGCAGATG TTGTGTTTTT GGTGGACAGC TCTGATCGCC TGGGATCCAA GTCCTTCCCA 
TTTGTGAAAA TGTTCATCAC CAAAATGATC AGCAGTCTCC CCATAGAGGC CGACAAATAC 
CGTGTGGCCC TGGCCCAGTA CAGTGATAAA CTTCACAGTG AATTCCACCT GAGCACCTTC 
AAAGGCAGGA GCCCCATGCT GAACCACCTA AGGAAGAACT TTGGATTCAT TGGCGGGTCC 
CTGCAGATAG GAAAGGCTCT TCAGGAGGCT CACAGGACTT ATTTCTCTGC ACCCGCAAAT 
GGGAGAGACA AGAAACAGTT TCCCCCAATT CTAGTGGTCC TGGCTTCATC TGAGTCTGAG 
GATAATGTGG AAGAGGCATC AAAGGCCCTG CGGAAAGACG GAGTGAAAAT CATCTCTGTA 
GGGGTGCAGA AAGCTTCTGA GGAAAACCTG AAGGCCATGG CCACGTCTCA GTTTCATTTC 
AACCTTCGGA CAGTCAGAGA CCTCAGCATG TTTTCCCAAA ACATGACACA CATCATCAAG 
GATGTAATAA AGTACAAGGA GGGAGCAGTT GATGACATCT TTGTAGAAGC TTGCCAAGGC 
CCTTCTATGG CCGATGTTGT GTTCCTATTG GATATGTCAA TCAATGGAAG TGAGGAGAAC 
TTTGACTATC TTAAAGGATT CTTGGAAGAA AGTGTATCTG CCCTTGACAT AAAGGAAAAT 
TGCATGAGGG TTGGCCTTGT GGCCTATAGC AATGAGACAA AAGTGATAAA TTCACTGAGC 
ATGGGCATAA ATAAGTCAGA GGTTCTCCAG CATATACAGA ACCTTTCTCC CCGAACTGGG 
AAGGCCTATA CTGGAGCTGC CATCAAAAAG CTCAGGAAGG AAGTTTTTAG TGCACGGAAT 
GGCAGTCGGA AGAATCAGGG GGTGCCCCAG ATTGCCGTGC TGGTGACCCA CCGAGATTCA 
GAAGACAACG TGACAAAAGC AGCTGTTAAC CTCCGACGGG AGGGTGTGAC CATCTTCACC 
CTGGGCATAG AGGGCGCCAG CGACACCCAG TTGGAAAAGA TAGCATCCCA CCCTGCTGAG 
CAGTATGTCT CCAAACTGAA GACCTTCGCT GACCTGGCTG CTCACAACCA GACATTTCTG 
AAGAAGCTGC GGAACCAAAT AACACACACA GTCTCTGTCT TTTCAGAGAG GACTGAAACG 
CTCAAATCTG GTTGTGTGGA CACTGAGGAA GCAGACATCT ATCTGCTTAT CGATGGCTCA 
GGGAGCACCC AGGCCACAGA TTTCCATGAA ATGAAGACGT TCCTGTCAGA GGTGGTAGGG 
ATGTTCAACA TTGCTCCCCA TAAGGTGCGG GTTGGGGCCG TTCAGTATGC TGACAGCTGG 
GACTTGGAAT TTGAGATCAA TAAATACTCC AACAAGCAGG ATTTGGGAAA GGCCATTGAG 
AATATCAGGC AGATGGGTGG GAATACAAAC ACAGGCGCAG CACTGAATTT CACACTGAGT 
CTGTTGCAAA AAGCAAAGAA GCAGCGAGGA AACAAAGTTC CATGCCACCT TGTTGTCCTG 
ACAAATGGCA TGTCCAAGGA TAGCATCTTG GAGCCTGCAA ACAGACTGAG AGAAGAGCAC 
ATCCGAGTTT ATGCTATCGG GATCAAGGAG GCCAACCAAA CACAGCTGAG AGAAATTGCA 
GGAGAGGAAA AGAGAGTGTA TTACGTGCAT GACTTTGATG CATTGAAAGA CATAAGAAAC 
CAAGTTGTTC AAGAAATCTG TACTGAAGAA GCTTGCAAAG AGATGAAAGC TGACATCATG 
TTTCTGGTGG ACAGTTCTGG AAGTATAGGA CCTGAAAACT TCAGCAAAAT GAAAACATTT 
ATGAAAAACC TGGTGAGCAA GTCTCAGATT GGACCAGATC GGGTGCAAAT TGGTGTAGTC 
CAGTTCAGCG ACATCAATAA GGAAGAGTTT CAGCTCAACA GATTCATGTC CCAAAGCGAC 
ATTTCAAATG CAATAGACCA AATGGCTCAC ATTGGACAAA CCACCCTGAC TGGTAGTGCC 
CTGAGCTTTG TGTCTCAGTA CTTCAGCCCC ACCAAGGGCG CCCGGCCCAA CATCAGAAAG 
TTTCTCATCC TCATCACGGA TGGTGAAGCT CAGGACATAG TAAAGGAACC AGCAGTAGTG 
CTTCGGCAAG AAGGTGTAAT CATCTATTCT GTGGGAGTGT TTGGCTCCAA TGTCACCCAG 
CTTGAGGAGA TCAGTGGGAG GCCCGAGATG GTTTTTTATG TTGAGAATTT TGACATTCTG 
CAGCGCATTG AAGATGATCT TGTTTTTGGA ATATGCAGCC CCCGTGAAGA ATGCAAGCGG 
ATTGAAGTTT TAGACGTTGT GTTTGTCATT GATAGCTCTG GCAGTATTGA CTATGATGAG 
TATAATATCA TGAAGGATTT TATGATTGGC TTAGTGAAAA AAGCTGATGT GGGCAAGAAT 
CAGGTCCGGT TTGGGGCTCT GAAGTATGCT GATGACCCAG AGGTGCTGTT TTATCTGGAT 
GACTTTGGCA CAAAACTGGA GGTAATTTCA GTGCTCCAGA ATGACCAAGC CATGGGTGGC 
AGTACTTATA CTGCTGAGGC ACTGGGCTTC TCAGACCACA TGTTCACTGA AGCCCGGGGC 
AGCCGCCTGA ACAAGGGGGT CCCCCAAGTC CTCATTGTGA TCACCGATGG GGAATCCCAT 
GATGCTGATA AACTCAATGC CACGGCAAAG GCCTTGCGGG ACAAAGGCAT TCTTGTCCTG 
GCTGTGGGGA TTGATGGTGC CAATCCCGTG GAGCTGTTAG CCATGGCAGG ATCAAGCGAC 
AAGTACTTCT TCGTGGAGAC TTTTGGAGGT CTGAAGGGAA TATTTTCAGA TGTGACAGCC 
AGTGTCTGCA ACTCTTCAAA AGTAGATTGT GAAATTGACA AAGTAGATCT TGTTTTCCTT 
ATGGATGGTT CAACTAGCAT TCAGCCAAAT GACTTCAAGA AAATGAAGGA ATTTCTGGCA 
TCTGTTGTTC AAGACTTTGA TGTCAGCCTC AACAGAGTGC GAATAGGAGC GGCCCAGTTT 
AGCGATACCT ATCACCCGGA GTTTCCACTG GGAACTTTCA TAGGTGAAAA AGAGATATCA 
TTTCAGATTG AAAACATCAA GCAGATCTTT GGAAACACAC ACATCGGTGC TGCACTCAGG 
GAGGTGGAAC ATTACTTCAG GCCAGACATG GGCAGCAGGA TAAATACAGG TACCCCACAG 
GTGCTGCTGG TCCTTACAGA TGGCCAGTCC CAAGACGAGG TGGCCCAGGC CGCGGAAGCC 
CTGAGACACA GAGGTATCGA CATCTACTCC GTGGGCATTG GGGATGTGGA TGACCAGCAG 
CTCATTCAGA TCACCGGGAC TGCAGAGAAA AAACTGACAG TGCACAACTT CGATGAACTG 
AAGAAGGTCA ATAAAAGGAT CGTTCGCAAC ATCTGTACCA CAGCGGGTGA AAGCAACTGT 
TTCGTGGATG TTGTGGTGGG ATTTGATGTC TCAACTCAGG AGAAAGGGCA GACTTTGCTT 
GAAGGTCAGC CTTGGATGGA AACCTACCTT CAAGACATCT TACGTGCCAT CAGCTCCCTC 
AATGGAGTAA GCTGTGAGGT GGGCACAGAG ACTCAGGTCA GTGTGGCTTT TCAAGTGACC 
AATGCCATGG AAAAATATTC TCCCAAGTTT GAGATCTACA GTGAAAACAT ACTGAATAGC 
TTGAAGGATA TAACAGTTAA AGGACCATCT CTTCTCAATG CAAACCTCTT GGATTCTCTA 
TGGGATACAT TTCAGAATAA ATCAGCTGCT CGAGGAAAGG TGGTCCTTTT ATTTTCAGAT 
GGATTGGATG ATGATGTTGA GAAACTTGAA CAAAAATCTG ATGAACTTAG AAAAGAAGGC 
CTGAATGCCC TCATAACTGT TGCTCTGGAT GGACCTGCTG ATTCAAGTGA CTTGGCTGAT 
CTTCCCTATA TTGAATTTGG GAAAGGATTT GAGTACAGGA CACAGCTCTC TATTGGCATG 
AGAGAACTTG GAAGCCGGCT GTCAAAGCAG CTGGTCAATG TTGCTGAAAG GACATGCTGC 
TGTTTGTTCT GCAAGTGCAT TGGAGGAGAT GGCACAATGG GAGATCCTGG ACCACCAGGG 
AAAAGGGGAC CTCCAGGTTT TAAAGGCAGT GAAGGCTACC TGGGAGAGGA GGGAATCGCT 
GGAGAAAGAG GAGCCCCTGG ACCAGTGGGA GAGCAAGGTA CTAAGGGATG CTATGGCACC 
AAAGGTCCTA AGGGAAACAG AGGACTAAAT GGACAGGAGG GAGAAGTTGG GGAAAATGGA 
ATTGACGGAT TAAACGGAGA ACAGGGTGAT AATGGTCTTC CTGGAAGAAA AGGAGAAAAG 
GGAGATGAGG GATCTCAGGG AAGCCCAGGG AAGAGAGGGA CTCCTGGTGA CCGTGGAGCA 
AAGGGCCTGC GAGGGGATCC CGGAGCTCCT GGAGTTGACA GTAGCATAGA AGGACCCACA 
GGCTTGAAAG GAGAACGTGG AAGACAAGGC AGAAGAGGCT GGCCAGGCCC CCCCGGGACA 
CCAGGCTCCA GAAGAAAGAC AGCAGCTCAT GGCAGAAGGG GACATACAGG CCCACAGGGA 
ACAGCAGGCA TCCCAGGACC AGATGGACTT GAAGGCTCCC TGGGACTTAA GGGCCCTCAG 
GGCCCAAGAG GAGAGGCTGG TGTGAAAGGA GAAAAAGGAG GTGTGGGAAG TAAAGGTCCC 
CAGGGGCCTC CAGGACCCGG AGGAGAGGCA GGGAATCAAG GCCGTTTGGG AAGCCAAGGA 
AATAAAGGAG AACCTGGAGA TCTGGGAGAA AAAGGAGCTG TTGGCTTTCC TGGTCCTCGT 
GGCTTGCAGG GCAATGATGG CAGTCCAGGT TATGGTAGTG TCGGACGCAA GGGAGCAAAG 
GGACAAGAAG GATTCCCTGG AGAAAGTGGA CCTAAGGGTG AGATTGGGGA CCCTGGTGGT 
CCAGGAGAGA CTGGGCTGAA GGGAGCTAGA GGCAAAATGA TATCTGCTGG GCTTCCAGGA 
GAGATGGGAT CCCCTGGGGA ACCAGGACCT CCTGGACGTA AGGGTGTGAA AGGAGCCAAA 
GGCTTGGCTT CATTTTCTAC ATGTGAGCTC ATTCAGTATG TGCGAGACCG CAGTCCTGGC 
AGGCATGGAA AACCGGAATG CCCAGTGCAC CCAACCGAGT TGGTGTTTGC CCTGGACCAC 
TCCCGGGATG TCACTGAGCA GGAATTTGAG CGGATGAAGG AGATGATGGC TTTCCTGGTG 
AGAGACATTA AGGTCCGGGA GAACAGCTGC CCCGTGGGAG CGCACATCGC CATCCTCTCC 
TATAACTCCC ACGCCAGGCA CCTTGTGCGC TTCTCAGACG CCTACAAGAA GAGTCAACTT 
CTCAGAGAAA TTGAAACTAT TCCTTATGAG AGATCCTCTG CCAGCAGGGA GATTGGCAGA 
GCAATGCGGT TTATTTCCAG GAATGTCTTC AAGCGGACGC TTCCGGGGGC ACACACGAGA 
AAAATCGCCA CATTTTTCAG CAGCGGTCAG TCCGCGGATG CCCACTCCAT CACCACGGCT 
GCCATGGAGT TCGGCGCGCT TGAAATCATT CCCGTGGTGA TCACTTTCAG CAACGTGCCC 
TCGGTCAGGC GCGCATTTGC GATTGACGAC ACTGGCACAT TTCAAGTAAT AGTGGTTCCC 
TCCGGGGCCG ACTACATACC AGCATTAGAG AGACTCCAGC GGTGCACTTT CTGCTATGAT 
GTGTGCAAGC CAGATGCTTC TTGTGACCAA GCCAGACCAC CCCCTGTGCA GTCTTACATG 
GATGCTGCTT TCCTTCTGGA TGCCTCCCGG AACATGGGAA GTGCTGAATT TGAAGACATA 
AGAGCCTTCC TTGGAGCACT ATTAGATCAC TTTGAAATCA CCCCAGAGCC GGAGACTTCT 
GTCACTGGAG ACCGGGTGGC CCTATTGAGC CATGCTCCCC CCGACTTCCT ACCCAACACT 
CAGAAGAGTC CAGTTAGAGC TGAGTTCAAT CTTACCACCT ACAGAAGTAA GCGCCTCATG 
AAGAGGCATG TGCACGAGTC AGTTAAACAA CTAAATGGAG ATGCTTTTAT TGGTCATGCC 
TTACAGTGGA CTCTGGACAA TGTATTTTTA AGTACACCCA ATCTGAGAAG AAACAAAGTC 
ATATTTGTGA TATCTGCTGG GGAAACCAGC CACTTAGATG GGGAAATCTT AAAGAAGGAA 
TCCTTGCGAG CCAAATGTCA GGGATATGCC TTATTTGTGT TTTCCCTTGG CCCTATTTGG 
GATGACAAGG AACTGGAGGA TCTCGCCAGC CACCCTTTGG ATCACCACCT GGTCCAGCTT 
GGCCGAATTC ATAAACCTGA CCACAGTTAT GGTGTGAAGT TTGTGAAGTC CTTTATAAAC 
TCAATCAGGC GTGCAATCAA CAAATATCCA CCAATAAACT TAAAAATAAA GTGCAACAGA 
CTTAACTCTA TAGATCCAAA GCAGCCCCCA CGACCATTCC GAAGCTTTGT TCCTGGACCA 
CTTAAAGCTA CCCTCAAAGA AGATGTATTA CAGAAGGCAA AATTCTTTCA AGATAAAAAA 
TATCTTTCAA GAGTAGCAAG AAGTGGCAGA GATGATGCTA TTCAAAATTT TATGAGAAGC 
ACCTCCCATA CCTTTAAGAA TGGAAGGATG ATAGAAAGTG CTCCCAAACA ACATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510725.1
CDS	208..7047
Translation	MEVSPRFEVEDFSGHNMMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_011512423.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512423.3

ORF Insert Sequence:

ATGGAAGTTT CCCCAAGATT TGAAGTTGAA GATTTTTCAG GTCATAATAT GATGTTGCTA 
ATTTTGTTCC TCGTGATAAT TTGTTCCCAT ATTTCTGTGA ACCAAGATTC CGGCCCTGAG 
TATGCAGATG TTGTGTTTTT GGTGGACAGC TCTGATCGCC TGGGATCCAA GTCCTTCCCA 
TTTGTGAAAA TGTTCATCAC CAAAATGATC AGCAGTCTCC CCATAGAGGC CGACAAATAC 
CGTGTGGCCC TGGCCCAGTA CAGTGATAAA CTTCACAGTG AATTCCACCT GAGCACCTTC 
AAAGGCAGGA GCCCCATGCT GAACCACCTA AGGAAGAACT TTGGATTCAT TGGCGGGTCC 
CTGCAGATAG GAAAGGCTCT TCAGGAGGCT CACAGGACTT ATTTCTCTGC ACCCGCAAAT 
GGGAGAGACA AGAAACAGTT TCCCCCAATT CTAGTGGTCC TGGCTTCATC TGAGTCTGAG 
GATAATGTGG AAGAGGCATC AAAGGCCCTG CGGAAAGACG GAGTGAAAAT CATCTCTGTA 
GGGGTGCAGA AAGCTTCTGA GGAAAACCTG AAGGCCATGG CCACGTCTCA GTTTCATTTC 
AACCTTCGGA CAGTCAGAGA CCTCAGCATG TTTTCCCAAA ACATGACACA CATCATCAAG 
GATGTAATAA AGTACAAGGA GGGAGCAGTT GATGACATCT TTGTAGAAGC TTGCCAAGGC 
CCTTCTATGG CCGATGTTGT GTTCCTATTG GATATGTCAA TCAATGGAAG TGAGGAGAAC 
TTTGACTATC TTAAAGGATT CTTGGAAGAA AGTGTATCTG CCCTTGACAT AAAGGAAAAT 
TGCATGAGGG TTGGCCTTGT GGCCTATAGC AATGAGACAA AAGTGATAAA TTCACTGAGC 
ATGGGCATAA ATAAGTCAGA GGTTCTCCAG CATATACAGA ACCTTTCTCC CCGAACTGGG 
AAGGCCTATA CTGGAGCTGC CATCAAAAAG CTCAGGAAGG AAGTTTTTAG TGCACGGAAT 
GGCAGTCGGA AGAATCAGGG GGTGCCCCAG ATTGCCGTGC TGGTGACCCA CCGAGATTCA 
GAAGACAACG TGACAAAAGC AGCTGTTAAC CTCCGACGGG AGGGTGTGAC CATCTTCACC 
CTGGGCATAG AGGGCGCCAG CGACACCCAG TTGGAAAAGA TAGCATCCCA CCCTGCTGAG 
CAGTATGTCT CCAAACTGAA GACCTTCGCT GACCTGGCTG CTCACAACCA GACATTTCTG 
AAGAAGCTGC GGAACCAAAT AACACACACA GTCTCTGTCT TTTCAGAGAG GACTGAAACG 
CTCAAATCTG GTTGTGTGGA CACTGAGGAA GCAGACATCT ATCTGCTTAT CGATGGCTCA 
GGGAGCACCC AGGCCACAGA TTTCCATGAA ATGAAGACGT TCCTGTCAGA GGTGGTAGGG 
ATGTTCAACA TTGCTCCCCA TAAGGTGCGG GTTGGGGCCG TTCAGTATGC TGACAGCTGG 
GACTTGGAAT TTGAGATCAA TAAATACTCC AACAAGCAGG ATTTGGGAAA GGCCATTGAG 
AATATCAGGC AGATGGGTGG GAATACAAAC ACAGGCGCAG CACTGAATTT CACACTGAGT 
CTGTTGCAAA AAGCAAAGAA GCAGCGAGGA AACAAAGTTC CATGCCACCT TGTTGTCCTG 
ACAAATGGCA TGTCCAAGGA TAGCATCTTG GAGCCTGCAA ACAGACTGAG AGAAGAGCAC 
ATCCGAGTTT ATGCTATCGG GATCAAGGAG GCCAACCAAA CACAGCTGAG AGAAATTGCA 
GGAGAGGAAA AGAGAGTGTA TTACGTGCAT GACTTTGATG CATTGAAAGA CATAAGAAAC 
CAAGTTGTTC AAGAAATCTG TACTGAAGAA GCTTGCAAAG AGATGAAAGC TGACATCATG 
TTTCTGGTGG ACAGTTCTGG AAGTATAGGA CCTGAAAACT TCAGCAAAAT GAAAACATTT 
ATGAAAAACC TGGTGAGCAA GTCTCAGATT GGACCAGATC GGGTGCAAAT TGGTGTAGTC 
CAGTTCAGCG ACATCAATAA GGAAGAGTTT CAGCTCAACA GATTCATGTC CCAAAGCGAC 
ATTTCAAATG CAATAGACCA AATGGCTCAC ATTGGACAAA CCACCCTGAC TGGTAGTGCC 
CTGAGCTTTG TGTCTCAGTA CTTCAGCCCC ACCAAGGGCG CCCGGCCCAA CATCAGAAAG 
TTTCTCATCC TCATCACGGA TGGTGAAGCT CAGGACATAG TAAAGGAACC AGCAGTAGTG 
CTTCGGCAAG AAGGTGTAAT CATCTATTCT GTGGGAGTGT TTGGCTCCAA TGTCACCCAG 
CTTGAGGAGA TCAGTGGGAG GCCCGAGATG GTTTTTTATG TTGAGAATTT TGACATTCTG 
CAGCGCATTG AAGATGATCT TGTTTTTGGA ATATGCAGCC CCCGTGAAGA ATGCAAGCGG 
ATTGAAGTTT TAGACGTTGT GTTTGTCATT GATAGCTCTG GCAGTATTGA CTATGATGAG 
TATAATATCA TGAAGGATTT TATGATTGGC TTAGTGAAAA AAGCTGATGT GGGCAAGAAT 
CAGGTCCGGT TTGGGGCTCT GAAGTATGCT GATGACCCAG AGGTGCTGTT TTATCTGGAT 
GACTTTGGCA CAAAACTGGA GGTAATTTCA GTGCTCCAGA ATGACCAAGC CATGGGTGGC 
AGTACTTATA CTGCTGAGGC ACTGGGCTTC TCAGACCACA TGTTCACTGA AGCCCGGGGC 
AGCCGCCTGA ACAAGGGGGT CCCCCAAGTC CTCATTGTGA TCACCGATGG GGAATCCCAT 
GATGCTGATA AACTCAATGC CACGGCAAAG GCCTTGCGGG ACAAAGGCAT TCTTGTCCTG 
GCTGTGGGGA TTGATGGTGC CAATCCCGTG GAGCTGTTAG CCATGGCAGG ATCAAGCGAC 
AAGTACTTCT TCGTGGAGAC TTTTGGAGGT CTGAAGGGAA TATTTTCAGA TGTGACAGCC 
AGTGTCTGCA ACTCTTCAAA AGTAGATTGT GAAATTGACA AAGTAGATCT TGTTTTCCTT 
ATGGATGGTT CAACTAGCAT TCAGCCAAAT GACTTCAAGA AAATGAAGGA ATTTCTGGCA 
TCTGTTGTTC AAGACTTTGA TGTCAGCCTC AACAGAGTGC GAATAGGAGC GGCCCAGTTT 
AGCGATACCT ATCACCCGGA GTTTCCACTG GGAACTTTCA TAGGTGAAAA AGAGATATCA 
TTTCAGATTG AAAACATCAA GCAGATCTTT GGAAACACAC ACATCGGTGC TGCACTCAGG 
GAGGTGGAAC ATTACTTCAG GCCAGACATG GGCAGCAGGA TAAATACAGG TACCCCACAG 
GTGCTGCTGG TCCTTACAGA TGGCCAGTCC CAAGACGAGG TGGCCCAGGC CGCGGAAGCC 
CTGAGACACA GAGGTATCGA CATCTACTCC GTGGGCATTG GGGATGTGGA TGACCAGCAG 
CTCATTCAGA TCACCGGGAC TGCAGAGAAA AAACTGACAG TGCACAACTT CGATGAACTG 
AAGAAGGTCA ATAAAAGGAT CGTTCGCAAC ATCTGTACCA CAGCGGGTGA AAGCAACTGT 
TTCGTGGATG TTGTGGTGGG ATTTGATGTC TCAACTCAGG AGAAAGGGCA GACTTTGCTT 
GAAGGTCAGC CTTGGATGGA AACCTACCTT CAAGACATCT TACGTGCCAT CAGCTCCCTC 
AATGGAGTAA GCTGTGAGGT GGGCACAGAG ACTCAGGTCA GTGTGGCTTT TCAAGTGACC 
AATGCCATGG AAAAATATTC TCCCAAGTTT GAGATCTACA GTGAAAACAT ACTGAATAGC 
TTGAAGGATA TAACAGTTAA AGGACCATCT CTTCTCAATG CAAACCTCTT GGATTCTCTA 
TGGGATACAT TTCAGAATAA ATCAGCTGCT CGAGGAAAGG TGGTCCTTTT ATTTTCAGAT 
GGATTGGATG ATGATGTTGA GAAACTTGAA CAAAAATCTG ATGAACTTAG AAAAGAAGGC 
CTGAATGCCC TCATAACTGT TGCTCTGGAT GGACCTGCTG ATTCAAGTGA CTTGGCTGAT 
CTTCCCTATA TTGAATTTGG GAAAGGATTT GAGTACAGGA CACAGCTCTC TATTGGCATG 
AGAGAACTTG GAAGCCGGCT GTCAAAGCAG CTGGTCAATG TTGCTGAAAG GACATGCTGC 
TGTTTGTTCT GCAAGTGCAT TGGAGGAGAT GGCACAATGG GAGATCCTGG ACCACCAGGG 
AAAAGGGGAC CTCCAGGTTT TAAAGGCAGT GAAGGCTACC TGGGAGAGGA GGGAATCGCT 
GGAGAAAGAG GAGCCCCTGG ACCAGTGGGA GAGCAAGGTA CTAAGGGATG CTATGGCACC 
AAAGGTCCTA AGGGAAACAG AGGACTAAAT GGACAGGAGG GAGAAGTTGG GGAAAATGGA 
ATTGACGGAT TAAACGGAGA ACAGGGTGAT AATGGTCTTC CTGGAAGAAA AGGAGAAAAG 
GGAGATGAGG GATCTCAGGG AAGCCCAGGG AAGAGAGGGA CTCCTGGTGA CCGTGGAGCA 
AAGGGCCTGC GAGGGGATCC CGGAGCTCCT GGAGTTGACA GTAGCATAGA AGGACCCACA 
GGCTTGAAAG GAGAACGTGG AAGACAAGGC AGAAGAGGCT GGCCAGGCCC CCCCGGGACA 
CCAGGCTCCA GAAGAAAGAC AGCAGCTCAT GGCAGAAGGG GACATACAGG CCCACAGGGA 
ACAGCAGGCA TCCCAGGACC AGATGGACTT GAAGGCTCCC TGGGACTTAA GGGCCCTCAG 
GGCCCAAGAG GAGAGGCTGG TGTGAAAGGA GAAAAAGGAG GTGTGGGAAG TAAAGGTCCC 
CAGGGGCCTC CAGGACCCGG AGGAGAGGCA GGGAATCAAG GCCGTTTGGG AAGCCAAGGA 
AATAAAGGAG AACCTGGAGA TCTGGGAGAA AAAGGAGCTG TTGGCTTTCC TGGTCCTCGT 
GGCTTGCAGG GCAATGATGG CAGTCCAGGT TATGGTAGTG TCGGACGCAA GGGAGCAAAG 
GGACAAGAAG GATTCCCTGG AGAAAGTGGA CCTAAGGGTG AGATTGGGGA CCCTGGTGGT 
CCAGGAGAGA CTGGGCTGAA GGGAGCTAGA GGCAAAATGA TATCTGCTGG GCTTCCAGGA 
GAGATGGGAT CCCCTGGGGA ACCAGGACCT CCTGGACGTA AGGGTGTGAA AGGAGCCAAA 
GGCTTGGCTT CATTTTCTAC ATGTGAGCTC ATTCAGTATG TGCGAGACCG CAGTCCTGGC 
AGGCATGGAA AACCGGAATG CCCAGTGCAC CCAACCGAGT TGGTGTTTGC CCTGGACCAC 
TCCCGGGATG TCACTGAGCA GGAATTTGAG CGGATGAAGG AGATGATGGC TTTCCTGGTG 
AGAGACATTA AGGTCCGGGA GAACAGCTGC CCCGTGGGAG CGCACATCGC CATCCTCTCC 
TATAACTCCC ACGCCAGGCA CCTTGTGCGC TTCTCAGACG CCTACAAGAA GAGTCAACTT 
CTCAGAGAAA TTGAAACTAT TCCTTATGAG AGATCCTCTG CCAGCAGGGA GATTGGCAGA 
GCAATGCGGT TTATTTCCAG GAATGTCTTC AAGCGGACGC TTCCGGGGGC ACACACGAGA 
AAAATCGCCA CATTTTTCAG CAGCGGTCAG TCCGCGGATG CCCACTCCAT CACCACGGCT 
GCCATGGAGT TCGGCGCGCT TGAAATCATT CCCGTGGTGA TCACTTTCAG CAACGTGCCC 
TCGGTCAGGC GCGCATTTGC GATTGACGAC ACTGGCACAT TTCAAGTAAT AGTGGTTCCC 
TCCGGGGCCG ACTACATACC AGCATTAGAG AGACTCCAGC GGTGCACTTT CTGCTATGAT 
GTGTGCAAGC CAGATGCTTC TTGTGACCAA GCCAGACCAC CCCCTGTGCA GTCTTACATG 
GATGCTGCTT TCCTTCTGGA TGCCTCCCGG AACATGGGAA GTGCTGAATT TGAAGACATA 
AGAGCCTTCC TTGGAGCACT ATTAGATCAC TTTGAAATCA CCCCAGAGCC GGAGACTTCT 
GTCACTGGAG ACCGGGTGGC CCTATTGAGC CATGCTCCCC CCGACTTCCT ACCCAACACT 
CAGAAGAGTC CAGTTAGAGC TGAGTTCAAT CTTACCACCT ACAGAAGTAA GCGCCTCATG 
AAGAGGCATG TGCACGAGTC AGTTAAACAA CTAAATGGAG ATGCTTTTAT TGGTCATGCC 
TTACAGTGGA CTCTGGACAA TGTATTTTTA AGTACACCCA ATCTGAGAAG AAACAAAGTC 
ATATTTGTGA TATCTGCTGG GGAAACCAGC CACTTAGATG GGGAAATCTT AAAGAAGGAA 
TCCTTGCGAG CCAAATGTCA GGGATATGCC TTATTTGTGT TTTCCCTTGG CCCTATTTGG 
GATGACAAGG AACTGGAGGA TCTCGCCAGC CACCCTTTGG ATCACCACCT GGTCCAGCTT 
GGCCGAATTC ATAAACCTGA CCACAGTTAT GGTGTGAAGT TTGTGAAGTC CTTTATAAAC 
TCAATCAGGC GTGCAATCAA CAAATATCCA CCAATAAACT TAAAAATAAA GTGCAACAGA 
CTTAACTCTA TAGATCCAAA GCAGCCCCCA CGACCATTCC GAAGCTTTGT TCCTGGACCA 
CTTAAAGCTA CCCTCAAAGA AGATGTATTA CAGAAGGCAA AATTCTTTCA AGATAAAAAA 
TATCTTTCAA GAGTAGCAAG AAGTGGCAGA GATGATGCTA TTCAAAATTT TATGAGAAGC 
ACCTCCCATA CCTTTAAGAA TGGAAGGATG ATAGAAAGTG CTCCCAAACA ACATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_011512424.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510726.1 Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X2, mRNA.

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CloneID	OHu75652
Clone ID Related Accession (Same CDS sequence)	XM_011512423.3 , XM_011512424.2 , XM_011512423.2
Accession Version	XM_011512424.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512424.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTTT CCCCAAGATT TGAAGTTGAA GATTTTTCAG GTCATAATAT GATGTTGCTA 
ATTTTGTTCC TCGTGATAAT TTGTTCCCAT ATTTCTGTGA ACCAAGATTC CGGCCCTGAG 
TATGCAGATG TTGTGTTTTT GGTGGACAGC TCTGATCGCC TGGGATCCAA GTCCTTCCCA 
TTTGTGAAAA TGTTCATCAC CAAAATGATC AGCAGTCTCC CCATAGAGGC CGACAAATAC 
CGTGTGGCCC TGGCCCAGTA CAGTGATAAA CTTCACAGTG AATTCCACCT GAGCACCTTC 
AAAGGCAGGA GCCCCATGCT GAACCACCTA AGGAAGAACT TTGGATTCAT TGGCGGGTCC 
CTGCAGATAG GAAAGGCTCT TCAGGAGGCT CACAGGACTT ATTTCTCTGC ACCCGCAAAT 
GGGAGAGACA AGAAACAGTT TCCCCCAATT CTAGTGGTCC TGGCTTCATC TGAGTCTGAG 
GATAATGTGG AAGAGGCATC AAAGGCCCTG CGGAAAGACG GAGTGAAAAT CATCTCTGTA 
GGGGTGCAGA AAGCTTCTGA GGAAAACCTG AAGGCCATGG CCACGTCTCA GTTTCATTTC 
AACCTTCGGA CAGTCAGAGA CCTCAGCATG TTTTCCCAAA ACATGACACA CATCATCAAG 
GATGTAATAA AGTACAAGGA GGGAGCAGTT GATGACATCT TTGTAGAAGC TTGCCAAGGC 
CCTTCTATGG CCGATGTTGT GTTCCTATTG GATATGTCAA TCAATGGAAG TGAGGAGAAC 
TTTGACTATC TTAAAGGATT CTTGGAAGAA AGTGTATCTG CCCTTGACAT AAAGGAAAAT 
TGCATGAGGG TTGGCCTTGT GGCCTATAGC AATGAGACAA AAGTGATAAA TTCACTGAGC 
ATGGGCATAA ATAAGTCAGA GGTTCTCCAG CATATACAGA ACCTTTCTCC CCGAACTGGG 
AAGGCCTATA CTGGAGCTGC CATCAAAAAG CTCAGGAAGG AAGTTTTTAG TGCACGGAAT 
GGCAGTCGGA AGAATCAGGG GGTGCCCCAG ATTGCCGTGC TGGTGACCCA CCGAGATTCA 
GAAGACAACG TGACAAAAGC AGCTGTTAAC CTCCGACGGG AGGGTGTGAC CATCTTCACC 
CTGGGCATAG AGGGCGCCAG CGACACCCAG TTGGAAAAGA TAGCATCCCA CCCTGCTGAG 
CAGTATGTCT CCAAACTGAA GACCTTCGCT GACCTGGCTG CTCACAACCA GACATTTCTG 
AAGAAGCTGC GGAACCAAAT AACACACACA GTCTCTGTCT TTTCAGAGAG GACTGAAACG 
CTCAAATCTG GTTGTGTGGA CACTGAGGAA GCAGACATCT ATCTGCTTAT CGATGGCTCA 
GGGAGCACCC AGGCCACAGA TTTCCATGAA ATGAAGACGT TCCTGTCAGA GGTGGTAGGG 
ATGTTCAACA TTGCTCCCCA TAAGGTGCGG GTTGGGGCCG TTCAGTATGC TGACAGCTGG 
GACTTGGAAT TTGAGATCAA TAAATACTCC AACAAGCAGG ATTTGGGAAA GGCCATTGAG 
AATATCAGGC AGATGGGTGG GAATACAAAC ACAGGCGCAG CACTGAATTT CACACTGAGT 
CTGTTGCAAA AAGCAAAGAA GCAGCGAGGA AACAAAGTTC CATGCCACCT TGTTGTCCTG 
ACAAATGGCA TGTCCAAGGA TAGCATCTTG GAGCCTGCAA ACAGACTGAG AGAAGAGCAC 
ATCCGAGTTT ATGCTATCGG GATCAAGGAG GCCAACCAAA CACAGCTGAG AGAAATTGCA 
GGAGAGGAAA AGAGAGTGTA TTACGTGCAT GACTTTGATG CATTGAAAGA CATAAGAAAC 
CAAGTTGTTC AAGAAATCTG TACTGAAGAA GCTTGCAAAG AGATGAAAGC TGACATCATG 
TTTCTGGTGG ACAGTTCTGG AAGTATAGGA CCTGAAAACT TCAGCAAAAT GAAAACATTT 
ATGAAAAACC TGGTGAGCAA GTCTCAGATT GGACCAGATC GGGTGCAAAT TGGTGTAGTC 
CAGTTCAGCG ACATCAATAA GGAAGAGTTT CAGCTCAACA GATTCATGTC CCAAAGCGAC 
ATTTCAAATG CAATAGACCA AATGGCTCAC ATTGGACAAA CCACCCTGAC TGGTAGTGCC 
CTGAGCTTTG TGTCTCAGTA CTTCAGCCCC ACCAAGGGCG CCCGGCCCAA CATCAGAAAG 
TTTCTCATCC TCATCACGGA TGGTGAAGCT CAGGACATAG TAAAGGAACC AGCAGTAGTG 
CTTCGGCAAG AAGGTGTAAT CATCTATTCT GTGGGAGTGT TTGGCTCCAA TGTCACCCAG 
CTTGAGGAGA TCAGTGGGAG GCCCGAGATG GTTTTTTATG TTGAGAATTT TGACATTCTG 
CAGCGCATTG AAGATGATCT TGTTTTTGGA ATATGCAGCC CCCGTGAAGA ATGCAAGCGG 
ATTGAAGTTT TAGACGTTGT GTTTGTCATT GATAGCTCTG GCAGTATTGA CTATGATGAG 
TATAATATCA TGAAGGATTT TATGATTGGC TTAGTGAAAA AAGCTGATGT GGGCAAGAAT 
CAGGTCCGGT TTGGGGCTCT GAAGTATGCT GATGACCCAG AGGTGCTGTT TTATCTGGAT 
GACTTTGGCA CAAAACTGGA GGTAATTTCA GTGCTCCAGA ATGACCAAGC CATGGGTGGC 
AGTACTTATA CTGCTGAGGC ACTGGGCTTC TCAGACCACA TGTTCACTGA AGCCCGGGGC 
AGCCGCCTGA ACAAGGGGGT CCCCCAAGTC CTCATTGTGA TCACCGATGG GGAATCCCAT 
GATGCTGATA AACTCAATGC CACGGCAAAG GCCTTGCGGG ACAAAGGCAT TCTTGTCCTG 
GCTGTGGGGA TTGATGGTGC CAATCCCGTG GAGCTGTTAG CCATGGCAGG ATCAAGCGAC 
AAGTACTTCT TCGTGGAGAC TTTTGGAGGT CTGAAGGGAA TATTTTCAGA TGTGACAGCC 
AGTGTCTGCA ACTCTTCAAA AGTAGATTGT GAAATTGACA AAGTAGATCT TGTTTTCCTT 
ATGGATGGTT CAACTAGCAT TCAGCCAAAT GACTTCAAGA AAATGAAGGA ATTTCTGGCA 
TCTGTTGTTC AAGACTTTGA TGTCAGCCTC AACAGAGTGC GAATAGGAGC GGCCCAGTTT 
AGCGATACCT ATCACCCGGA GTTTCCACTG GGAACTTTCA TAGGTGAAAA AGAGATATCA 
TTTCAGATTG AAAACATCAA GCAGATCTTT GGAAACACAC ACATCGGTGC TGCACTCAGG 
GAGGTGGAAC ATTACTTCAG GCCAGACATG GGCAGCAGGA TAAATACAGG TACCCCACAG 
GTGCTGCTGG TCCTTACAGA TGGCCAGTCC CAAGACGAGG TGGCCCAGGC CGCGGAAGCC 
CTGAGACACA GAGGTATCGA CATCTACTCC GTGGGCATTG GGGATGTGGA TGACCAGCAG 
CTCATTCAGA TCACCGGGAC TGCAGAGAAA AAACTGACAG TGCACAACTT CGATGAACTG 
AAGAAGGTCA ATAAAAGGAT CGTTCGCAAC ATCTGTACCA CAGCGGGTGA AAGCAACTGT 
TTCGTGGATG TTGTGGTGGG ATTTGATGTC TCAACTCAGG AGAAAGGGCA GACTTTGCTT 
GAAGGTCAGC CTTGGATGGA AACCTACCTT CAAGACATCT TACGTGCCAT CAGCTCCCTC 
AATGGAGTAA GCTGTGAGGT GGGCACAGAG ACTCAGGTCA GTGTGGCTTT TCAAGTGACC 
AATGCCATGG AAAAATATTC TCCCAAGTTT GAGATCTACA GTGAAAACAT ACTGAATAGC 
TTGAAGGATA TAACAGTTAA AGGACCATCT CTTCTCAATG CAAACCTCTT GGATTCTCTA 
TGGGATACAT TTCAGAATAA ATCAGCTGCT CGAGGAAAGG TGGTCCTTTT ATTTTCAGAT 
GGATTGGATG ATGATGTTGA GAAACTTGAA CAAAAATCTG ATGAACTTAG AAAAGAAGGC 
CTGAATGCCC TCATAACTGT TGCTCTGGAT GGACCTGCTG ATTCAAGTGA CTTGGCTGAT 
CTTCCCTATA TTGAATTTGG GAAAGGATTT GAGTACAGGA CACAGCTCTC TATTGGCATG 
AGAGAACTTG GAAGCCGGCT GTCAAAGCAG CTGGTCAATG TTGCTGAAAG GACATGCTGC 
TGTTTGTTCT GCAAGTGCAT TGGAGGAGAT GGCACAATGG GAGATCCTGG ACCACCAGGG 
AAAAGGGGAC CTCCAGGTTT TAAAGGCAGT GAAGGCTACC TGGGAGAGGA GGGAATCGCT 
GGAGAAAGAG GAGCCCCTGG ACCAGTGGGA GAGCAAGGTA CTAAGGGATG CTATGGCACC 
AAAGGTCCTA AGGGAAACAG AGGACTAAAT GGACAGGAGG GAGAAGTTGG GGAAAATGGA 
ATTGACGGAT TAAACGGAGA ACAGGGTGAT AATGGTCTTC CTGGAAGAAA AGGAGAAAAG 
GGAGATGAGG GATCTCAGGG AAGCCCAGGG AAGAGAGGGA CTCCTGGTGA CCGTGGAGCA 
AAGGGCCTGC GAGGGGATCC CGGAGCTCCT GGAGTTGACA GTAGCATAGA AGGACCCACA 
GGCTTGAAAG GAGAACGTGG AAGACAAGGC AGAAGAGGCT GGCCAGGCCC CCCCGGGACA 
CCAGGCTCCA GAAGAAAGAC AGCAGCTCAT GGCAGAAGGG GACATACAGG CCCACAGGGA 
ACAGCAGGCA TCCCAGGACC AGATGGACTT GAAGGCTCCC TGGGACTTAA GGGCCCTCAG 
GGCCCAAGAG GAGAGGCTGG TGTGAAAGGA GAAAAAGGAG GTGTGGGAAG TAAAGGTCCC 
CAGGGGCCTC CAGGACCCGG AGGAGAGGCA GGGAATCAAG GCCGTTTGGG AAGCCAAGGA 
AATAAAGGAG AACCTGGAGA TCTGGGAGAA AAAGGAGCTG TTGGCTTTCC TGGTCCTCGT 
GGCTTGCAGG GCAATGATGG CAGTCCAGGT TATGGTAGTG TCGGACGCAA GGGAGCAAAG 
GGACAAGAAG GATTCCCTGG AGAAAGTGGA CCTAAGGGTG AGATTGGGGA CCCTGGTGGT 
CCAGGAGAGA CTGGGCTGAA GGGAGCTAGA GGCAAAATGA TATCTGCTGG GCTTCCAGGA 
GAGATGGGAT CCCCTGGGGA ACCAGGACCT CCTGGACGTA AGGGTGTGAA AGGAGCCAAA 
GGCTTGGCTT CATTTTCTAC ATGTGAGCTC ATTCAGTATG TGCGAGACCG CAGTCCTGGC 
AGGCATGGAA AACCGGAATG CCCAGTGCAC CCAACCGAGT TGGTGTTTGC CCTGGACCAC 
TCCCGGGATG TCACTGAGCA GGAATTTGAG CGGATGAAGG AGATGATGGC TTTCCTGGTG 
AGAGACATTA AGGTCCGGGA GAACAGCTGC CCCGTGGGAG CGCACATCGC CATCCTCTCC 
TATAACTCCC ACGCCAGGCA CCTTGTGCGC TTCTCAGACG CCTACAAGAA GAGTCAACTT 
CTCAGAGAAA TTGAAACTAT TCCTTATGAG AGATCCTCTG CCAGCAGGGA GATTGGCAGA 
GCAATGCGGT TTATTTCCAG GAATGTCTTC AAGCGGACGC TTCCGGGGGC ACACACGAGA 
AAAATCGCCA CATTTTTCAG CAGCGGTCAG TCCGCGGATG CCCACTCCAT CACCACGGCT 
GCCATGGAGT TCGGCGCGCT TGAAATCATT CCCGTGGTGA TCACTTTCAG CAACGTGCCC 
TCGGTCAGGC GCGCATTTGC GATTGACGAC ACTGGCACAT TTCAAGTAAT AGTGGTTCCC 
TCCGGGGCCG ACTACATACC AGCATTAGAG AGACTCCAGC GGTGCACTTT CTGCTATGAT 
GTGTGCAAGC CAGATGCTTC TTGTGACCAA GCCAGACCAC CCCCTGTGCA GTCTTACATG 
GATGCTGCTT TCCTTCTGGA TGCCTCCCGG AACATGGGAA GTGCTGAATT TGAAGACATA 
AGAGCCTTCC TTGGAGCACT ATTAGATCAC TTTGAAATCA CCCCAGAGCC GGAGACTTCT 
GTCACTGGAG ACCGGGTGGC CCTATTGAGC CATGCTCCCC CCGACTTCCT ACCCAACACT 
CAGAAGAGTC CAGTTAGAGC TGAGTTCAAT CTTACCACCT ACAGAAGTAA GCGCCTCATG 
AAGAGGCATG TGCACGAGTC AGTTAAACAA CTAAATGGAG ATGCTTTTAT TGGTCATGCC 
TTACAGTGGA CTCTGGACAA TGTATTTTTA AGTACACCCA ATCTGAGAAG AAACAAAGTC 
ATATTTGTGA TATCTGCTGG GGAAACCAGC CACTTAGATG GGGAAATCTT AAAGAAGGAA 
TCCTTGCGAG CCAAATGTCA GGGATATGCC TTATTTGTGT TTTCCCTTGG CCCTATTTGG 
GATGACAAGG AACTGGAGGA TCTCGCCAGC CACCCTTTGG ATCACCACCT GGTCCAGCTT 
GGCCGAATTC ATAAACCTGA CCACAGTTAT GGTGTGAAGT TTGTGAAGTC CTTTATAAAC 
TCAATCAGGC GTGCAATCAA CAAATATCCA CCAATAAACT TAAAAATAAA GTGCAACAGA 
CTTAACTCTA TAGATCCAAA GCAGCCCCCA CGACCATTCC GAAGCTTTGT TCCTGGACCA 
CTTAAAGCTA CCCTCAAAGA AGATGTATTA CAGAAGGCAA AATTCTTTCA AGATAAAAAA 
TATCTTTCAA GAGTAGCAAG AAGTGGCAGA GATGATGCTA TTCAAAATTT TATGAGAAGC 
ACCTCCCATA CCTTTAAGAA TGGAAGGATG ATAGAAAGTG CTCCCAAACA ACATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510726.1
CDS	208..7047
Translation	MEVSPRFEVEDFSGHNMMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_011512424.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 chain (COL6A6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512424.2

ORF Insert Sequence:

ATGGAAGTTT CCCCAAGATT TGAAGTTGAA GATTTTTCAG GTCATAATAT GATGTTGCTA 
ATTTTGTTCC TCGTGATAAT TTGTTCCCAT ATTTCTGTGA ACCAAGATTC CGGCCCTGAG 
TATGCAGATG TTGTGTTTTT GGTGGACAGC TCTGATCGCC TGGGATCCAA GTCCTTCCCA 
TTTGTGAAAA TGTTCATCAC CAAAATGATC AGCAGTCTCC CCATAGAGGC CGACAAATAC 
CGTGTGGCCC TGGCCCAGTA CAGTGATAAA CTTCACAGTG AATTCCACCT GAGCACCTTC 
AAAGGCAGGA GCCCCATGCT GAACCACCTA AGGAAGAACT TTGGATTCAT TGGCGGGTCC 
CTGCAGATAG GAAAGGCTCT TCAGGAGGCT CACAGGACTT ATTTCTCTGC ACCCGCAAAT 
GGGAGAGACA AGAAACAGTT TCCCCCAATT CTAGTGGTCC TGGCTTCATC TGAGTCTGAG 
GATAATGTGG AAGAGGCATC AAAGGCCCTG CGGAAAGACG GAGTGAAAAT CATCTCTGTA 
GGGGTGCAGA AAGCTTCTGA GGAAAACCTG AAGGCCATGG CCACGTCTCA GTTTCATTTC 
AACCTTCGGA CAGTCAGAGA CCTCAGCATG TTTTCCCAAA ACATGACACA CATCATCAAG 
GATGTAATAA AGTACAAGGA GGGAGCAGTT GATGACATCT TTGTAGAAGC TTGCCAAGGC 
CCTTCTATGG CCGATGTTGT GTTCCTATTG GATATGTCAA TCAATGGAAG TGAGGAGAAC 
TTTGACTATC TTAAAGGATT CTTGGAAGAA AGTGTATCTG CCCTTGACAT AAAGGAAAAT 
TGCATGAGGG TTGGCCTTGT GGCCTATAGC AATGAGACAA AAGTGATAAA TTCACTGAGC 
ATGGGCATAA ATAAGTCAGA GGTTCTCCAG CATATACAGA ACCTTTCTCC CCGAACTGGG 
AAGGCCTATA CTGGAGCTGC CATCAAAAAG CTCAGGAAGG AAGTTTTTAG TGCACGGAAT 
GGCAGTCGGA AGAATCAGGG GGTGCCCCAG ATTGCCGTGC TGGTGACCCA CCGAGATTCA 
GAAGACAACG TGACAAAAGC AGCTGTTAAC CTCCGACGGG AGGGTGTGAC CATCTTCACC 
CTGGGCATAG AGGGCGCCAG CGACACCCAG TTGGAAAAGA TAGCATCCCA CCCTGCTGAG 
CAGTATGTCT CCAAACTGAA GACCTTCGCT GACCTGGCTG CTCACAACCA GACATTTCTG 
AAGAAGCTGC GGAACCAAAT AACACACACA GTCTCTGTCT TTTCAGAGAG GACTGAAACG 
CTCAAATCTG GTTGTGTGGA CACTGAGGAA GCAGACATCT ATCTGCTTAT CGATGGCTCA 
GGGAGCACCC AGGCCACAGA TTTCCATGAA ATGAAGACGT TCCTGTCAGA GGTGGTAGGG 
ATGTTCAACA TTGCTCCCCA TAAGGTGCGG GTTGGGGCCG TTCAGTATGC TGACAGCTGG 
GACTTGGAAT TTGAGATCAA TAAATACTCC AACAAGCAGG ATTTGGGAAA GGCCATTGAG 
AATATCAGGC AGATGGGTGG GAATACAAAC ACAGGCGCAG CACTGAATTT CACACTGAGT 
CTGTTGCAAA AAGCAAAGAA GCAGCGAGGA AACAAAGTTC CATGCCACCT TGTTGTCCTG 
ACAAATGGCA TGTCCAAGGA TAGCATCTTG GAGCCTGCAA ACAGACTGAG AGAAGAGCAC 
ATCCGAGTTT ATGCTATCGG GATCAAGGAG GCCAACCAAA CACAGCTGAG AGAAATTGCA 
GGAGAGGAAA AGAGAGTGTA TTACGTGCAT GACTTTGATG CATTGAAAGA CATAAGAAAC 
CAAGTTGTTC AAGAAATCTG TACTGAAGAA GCTTGCAAAG AGATGAAAGC TGACATCATG 
TTTCTGGTGG ACAGTTCTGG AAGTATAGGA CCTGAAAACT TCAGCAAAAT GAAAACATTT 
ATGAAAAACC TGGTGAGCAA GTCTCAGATT GGACCAGATC GGGTGCAAAT TGGTGTAGTC 
CAGTTCAGCG ACATCAATAA GGAAGAGTTT CAGCTCAACA GATTCATGTC CCAAAGCGAC 
ATTTCAAATG CAATAGACCA AATGGCTCAC ATTGGACAAA CCACCCTGAC TGGTAGTGCC 
CTGAGCTTTG TGTCTCAGTA CTTCAGCCCC ACCAAGGGCG CCCGGCCCAA CATCAGAAAG 
TTTCTCATCC TCATCACGGA TGGTGAAGCT CAGGACATAG TAAAGGAACC AGCAGTAGTG 
CTTCGGCAAG AAGGTGTAAT CATCTATTCT GTGGGAGTGT TTGGCTCCAA TGTCACCCAG 
CTTGAGGAGA TCAGTGGGAG GCCCGAGATG GTTTTTTATG TTGAGAATTT TGACATTCTG 
CAGCGCATTG AAGATGATCT TGTTTTTGGA ATATGCAGCC CCCGTGAAGA ATGCAAGCGG 
ATTGAAGTTT TAGACGTTGT GTTTGTCATT GATAGCTCTG GCAGTATTGA CTATGATGAG 
TATAATATCA TGAAGGATTT TATGATTGGC TTAGTGAAAA AAGCTGATGT GGGCAAGAAT 
CAGGTCCGGT TTGGGGCTCT GAAGTATGCT GATGACCCAG AGGTGCTGTT TTATCTGGAT 
GACTTTGGCA CAAAACTGGA GGTAATTTCA GTGCTCCAGA ATGACCAAGC CATGGGTGGC 
AGTACTTATA CTGCTGAGGC ACTGGGCTTC TCAGACCACA TGTTCACTGA AGCCCGGGGC 
AGCCGCCTGA ACAAGGGGGT CCCCCAAGTC CTCATTGTGA TCACCGATGG GGAATCCCAT 
GATGCTGATA AACTCAATGC CACGGCAAAG GCCTTGCGGG ACAAAGGCAT TCTTGTCCTG 
GCTGTGGGGA TTGATGGTGC CAATCCCGTG GAGCTGTTAG CCATGGCAGG ATCAAGCGAC 
AAGTACTTCT TCGTGGAGAC TTTTGGAGGT CTGAAGGGAA TATTTTCAGA TGTGACAGCC 
AGTGTCTGCA ACTCTTCAAA AGTAGATTGT GAAATTGACA AAGTAGATCT TGTTTTCCTT 
ATGGATGGTT CAACTAGCAT TCAGCCAAAT GACTTCAAGA AAATGAAGGA ATTTCTGGCA 
TCTGTTGTTC AAGACTTTGA TGTCAGCCTC AACAGAGTGC GAATAGGAGC GGCCCAGTTT 
AGCGATACCT ATCACCCGGA GTTTCCACTG GGAACTTTCA TAGGTGAAAA AGAGATATCA 
TTTCAGATTG AAAACATCAA GCAGATCTTT GGAAACACAC ACATCGGTGC TGCACTCAGG 
GAGGTGGAAC ATTACTTCAG GCCAGACATG GGCAGCAGGA TAAATACAGG TACCCCACAG 
GTGCTGCTGG TCCTTACAGA TGGCCAGTCC CAAGACGAGG TGGCCCAGGC CGCGGAAGCC 
CTGAGACACA GAGGTATCGA CATCTACTCC GTGGGCATTG GGGATGTGGA TGACCAGCAG 
CTCATTCAGA TCACCGGGAC TGCAGAGAAA AAACTGACAG TGCACAACTT CGATGAACTG 
AAGAAGGTCA ATAAAAGGAT CGTTCGCAAC ATCTGTACCA CAGCGGGTGA AAGCAACTGT 
TTCGTGGATG TTGTGGTGGG ATTTGATGTC TCAACTCAGG AGAAAGGGCA GACTTTGCTT 
GAAGGTCAGC CTTGGATGGA AACCTACCTT CAAGACATCT TACGTGCCAT CAGCTCCCTC 
AATGGAGTAA GCTGTGAGGT GGGCACAGAG ACTCAGGTCA GTGTGGCTTT TCAAGTGACC 
AATGCCATGG AAAAATATTC TCCCAAGTTT GAGATCTACA GTGAAAACAT ACTGAATAGC 
TTGAAGGATA TAACAGTTAA AGGACCATCT CTTCTCAATG CAAACCTCTT GGATTCTCTA 
TGGGATACAT TTCAGAATAA ATCAGCTGCT CGAGGAAAGG TGGTCCTTTT ATTTTCAGAT 
GGATTGGATG ATGATGTTGA GAAACTTGAA CAAAAATCTG ATGAACTTAG AAAAGAAGGC 
CTGAATGCCC TCATAACTGT TGCTCTGGAT GGACCTGCTG ATTCAAGTGA CTTGGCTGAT 
CTTCCCTATA TTGAATTTGG GAAAGGATTT GAGTACAGGA CACAGCTCTC TATTGGCATG 
AGAGAACTTG GAAGCCGGCT GTCAAAGCAG CTGGTCAATG TTGCTGAAAG GACATGCTGC 
TGTTTGTTCT GCAAGTGCAT TGGAGGAGAT GGCACAATGG GAGATCCTGG ACCACCAGGG 
AAAAGGGGAC CTCCAGGTTT TAAAGGCAGT GAAGGCTACC TGGGAGAGGA GGGAATCGCT 
GGAGAAAGAG GAGCCCCTGG ACCAGTGGGA GAGCAAGGTA CTAAGGGATG CTATGGCACC 
AAAGGTCCTA AGGGAAACAG AGGACTAAAT GGACAGGAGG GAGAAGTTGG GGAAAATGGA 
ATTGACGGAT TAAACGGAGA ACAGGGTGAT AATGGTCTTC CTGGAAGAAA AGGAGAAAAG 
GGAGATGAGG GATCTCAGGG AAGCCCAGGG AAGAGAGGGA CTCCTGGTGA CCGTGGAGCA 
AAGGGCCTGC GAGGGGATCC CGGAGCTCCT GGAGTTGACA GTAGCATAGA AGGACCCACA 
GGCTTGAAAG GAGAACGTGG AAGACAAGGC AGAAGAGGCT GGCCAGGCCC CCCCGGGACA 
CCAGGCTCCA GAAGAAAGAC AGCAGCTCAT GGCAGAAGGG GACATACAGG CCCACAGGGA 
ACAGCAGGCA TCCCAGGACC AGATGGACTT GAAGGCTCCC TGGGACTTAA GGGCCCTCAG 
GGCCCAAGAG GAGAGGCTGG TGTGAAAGGA GAAAAAGGAG GTGTGGGAAG TAAAGGTCCC 
CAGGGGCCTC CAGGACCCGG AGGAGAGGCA GGGAATCAAG GCCGTTTGGG AAGCCAAGGA 
AATAAAGGAG AACCTGGAGA TCTGGGAGAA AAAGGAGCTG TTGGCTTTCC TGGTCCTCGT 
GGCTTGCAGG GCAATGATGG CAGTCCAGGT TATGGTAGTG TCGGACGCAA GGGAGCAAAG 
GGACAAGAAG GATTCCCTGG AGAAAGTGGA CCTAAGGGTG AGATTGGGGA CCCTGGTGGT 
CCAGGAGAGA CTGGGCTGAA GGGAGCTAGA GGCAAAATGA TATCTGCTGG GCTTCCAGGA 
GAGATGGGAT CCCCTGGGGA ACCAGGACCT CCTGGACGTA AGGGTGTGAA AGGAGCCAAA 
GGCTTGGCTT CATTTTCTAC ATGTGAGCTC ATTCAGTATG TGCGAGACCG CAGTCCTGGC 
AGGCATGGAA AACCGGAATG CCCAGTGCAC CCAACCGAGT TGGTGTTTGC CCTGGACCAC 
TCCCGGGATG TCACTGAGCA GGAATTTGAG CGGATGAAGG AGATGATGGC TTTCCTGGTG 
AGAGACATTA AGGTCCGGGA GAACAGCTGC CCCGTGGGAG CGCACATCGC CATCCTCTCC 
TATAACTCCC ACGCCAGGCA CCTTGTGCGC TTCTCAGACG CCTACAAGAA GAGTCAACTT 
CTCAGAGAAA TTGAAACTAT TCCTTATGAG AGATCCTCTG CCAGCAGGGA GATTGGCAGA 
GCAATGCGGT TTATTTCCAG GAATGTCTTC AAGCGGACGC TTCCGGGGGC ACACACGAGA 
AAAATCGCCA CATTTTTCAG CAGCGGTCAG TCCGCGGATG CCCACTCCAT CACCACGGCT 
GCCATGGAGT TCGGCGCGCT TGAAATCATT CCCGTGGTGA TCACTTTCAG CAACGTGCCC 
TCGGTCAGGC GCGCATTTGC GATTGACGAC ACTGGCACAT TTCAAGTAAT AGTGGTTCCC 
TCCGGGGCCG ACTACATACC AGCATTAGAG AGACTCCAGC GGTGCACTTT CTGCTATGAT 
GTGTGCAAGC CAGATGCTTC TTGTGACCAA GCCAGACCAC CCCCTGTGCA GTCTTACATG 
GATGCTGCTT TCCTTCTGGA TGCCTCCCGG AACATGGGAA GTGCTGAATT TGAAGACATA 
AGAGCCTTCC TTGGAGCACT ATTAGATCAC TTTGAAATCA CCCCAGAGCC GGAGACTTCT 
GTCACTGGAG ACCGGGTGGC CCTATTGAGC CATGCTCCCC CCGACTTCCT ACCCAACACT 
CAGAAGAGTC CAGTTAGAGC TGAGTTCAAT CTTACCACCT ACAGAAGTAA GCGCCTCATG 
AAGAGGCATG TGCACGAGTC AGTTAAACAA CTAAATGGAG ATGCTTTTAT TGGTCATGCC 
TTACAGTGGA CTCTGGACAA TGTATTTTTA AGTACACCCA ATCTGAGAAG AAACAAAGTC 
ATATTTGTGA TATCTGCTGG GGAAACCAGC CACTTAGATG GGGAAATCTT AAAGAAGGAA 
TCCTTGCGAG CCAAATGTCA GGGATATGCC TTATTTGTGT TTTCCCTTGG CCCTATTTGG 
GATGACAAGG AACTGGAGGA TCTCGCCAGC CACCCTTTGG ATCACCACCT GGTCCAGCTT 
GGCCGAATTC ATAAACCTGA CCACAGTTAT GGTGTGAAGT TTGTGAAGTC CTTTATAAAC 
TCAATCAGGC GTGCAATCAA CAAATATCCA CCAATAAACT TAAAAATAAA GTGCAACAGA 
CTTAACTCTA TAGATCCAAA GCAGCCCCCA CGACCATTCC GAAGCTTTGT TCCTGGACCA 
CTTAAAGCTA CCCTCAAAGA AGATGTATTA CAGAAGGCAA AATTCTTTCA AGATAAAAAA 
TATCTTTCAA GAGTAGCAAG AAGTGGCAGA GATGATGCTA TTCAAAATTT TATGAGAAGC 
ACCTCCCATA CCTTTAAGAA TGGAAGGATG ATAGAAAGTG CTCCCAAACA ACATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_011512423.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510725.1 Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X1, mRNA.

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CloneID	OHu75652
Clone ID Related Accession (Same CDS sequence)	XM_011512423.3 , XM_011512424.2 , XM_011512423.2
Accession Version	XM_011512423.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512423.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTTT CCCCAAGATT TGAAGTTGAA GATTTTTCAG GTCATAATAT GATGTTGCTA 
ATTTTGTTCC TCGTGATAAT TTGTTCCCAT ATTTCTGTGA ACCAAGATTC CGGCCCTGAG 
TATGCAGATG TTGTGTTTTT GGTGGACAGC TCTGATCGCC TGGGATCCAA GTCCTTCCCA 
TTTGTGAAAA TGTTCATCAC CAAAATGATC AGCAGTCTCC CCATAGAGGC CGACAAATAC 
CGTGTGGCCC TGGCCCAGTA CAGTGATAAA CTTCACAGTG AATTCCACCT GAGCACCTTC 
AAAGGCAGGA GCCCCATGCT GAACCACCTA AGGAAGAACT TTGGATTCAT TGGCGGGTCC 
CTGCAGATAG GAAAGGCTCT TCAGGAGGCT CACAGGACTT ATTTCTCTGC ACCCGCAAAT 
GGGAGAGACA AGAAACAGTT TCCCCCAATT CTAGTGGTCC TGGCTTCATC TGAGTCTGAG 
GATAATGTGG AAGAGGCATC AAAGGCCCTG CGGAAAGACG GAGTGAAAAT CATCTCTGTA 
GGGGTGCAGA AAGCTTCTGA GGAAAACCTG AAGGCCATGG CCACGTCTCA GTTTCATTTC 
AACCTTCGGA CAGTCAGAGA CCTCAGCATG TTTTCCCAAA ACATGACACA CATCATCAAG 
GATGTAATAA AGTACAAGGA GGGAGCAGTT GATGACATCT TTGTAGAAGC TTGCCAAGGC 
CCTTCTATGG CCGATGTTGT GTTCCTATTG GATATGTCAA TCAATGGAAG TGAGGAGAAC 
TTTGACTATC TTAAAGGATT CTTGGAAGAA AGTGTATCTG CCCTTGACAT AAAGGAAAAT 
TGCATGAGGG TTGGCCTTGT GGCCTATAGC AATGAGACAA AAGTGATAAA TTCACTGAGC 
ATGGGCATAA ATAAGTCAGA GGTTCTCCAG CATATACAGA ACCTTTCTCC CCGAACTGGG 
AAGGCCTATA CTGGAGCTGC CATCAAAAAG CTCAGGAAGG AAGTTTTTAG TGCACGGAAT 
GGCAGTCGGA AGAATCAGGG GGTGCCCCAG ATTGCCGTGC TGGTGACCCA CCGAGATTCA 
GAAGACAACG TGACAAAAGC AGCTGTTAAC CTCCGACGGG AGGGTGTGAC CATCTTCACC 
CTGGGCATAG AGGGCGCCAG CGACACCCAG TTGGAAAAGA TAGCATCCCA CCCTGCTGAG 
CAGTATGTCT CCAAACTGAA GACCTTCGCT GACCTGGCTG CTCACAACCA GACATTTCTG 
AAGAAGCTGC GGAACCAAAT AACACACACA GTCTCTGTCT TTTCAGAGAG GACTGAAACG 
CTCAAATCTG GTTGTGTGGA CACTGAGGAA GCAGACATCT ATCTGCTTAT CGATGGCTCA 
GGGAGCACCC AGGCCACAGA TTTCCATGAA ATGAAGACGT TCCTGTCAGA GGTGGTAGGG 
ATGTTCAACA TTGCTCCCCA TAAGGTGCGG GTTGGGGCCG TTCAGTATGC TGACAGCTGG 
GACTTGGAAT TTGAGATCAA TAAATACTCC AACAAGCAGG ATTTGGGAAA GGCCATTGAG 
AATATCAGGC AGATGGGTGG GAATACAAAC ACAGGCGCAG CACTGAATTT CACACTGAGT 
CTGTTGCAAA AAGCAAAGAA GCAGCGAGGA AACAAAGTTC CATGCCACCT TGTTGTCCTG 
ACAAATGGCA TGTCCAAGGA TAGCATCTTG GAGCCTGCAA ACAGACTGAG AGAAGAGCAC 
ATCCGAGTTT ATGCTATCGG GATCAAGGAG GCCAACCAAA CACAGCTGAG AGAAATTGCA 
GGAGAGGAAA AGAGAGTGTA TTACGTGCAT GACTTTGATG CATTGAAAGA CATAAGAAAC 
CAAGTTGTTC AAGAAATCTG TACTGAAGAA GCTTGCAAAG AGATGAAAGC TGACATCATG 
TTTCTGGTGG ACAGTTCTGG AAGTATAGGA CCTGAAAACT TCAGCAAAAT GAAAACATTT 
ATGAAAAACC TGGTGAGCAA GTCTCAGATT GGACCAGATC GGGTGCAAAT TGGTGTAGTC 
CAGTTCAGCG ACATCAATAA GGAAGAGTTT CAGCTCAACA GATTCATGTC CCAAAGCGAC 
ATTTCAAATG CAATAGACCA AATGGCTCAC ATTGGACAAA CCACCCTGAC TGGTAGTGCC 
CTGAGCTTTG TGTCTCAGTA CTTCAGCCCC ACCAAGGGCG CCCGGCCCAA CATCAGAAAG 
TTTCTCATCC TCATCACGGA TGGTGAAGCT CAGGACATAG TAAAGGAACC AGCAGTAGTG 
CTTCGGCAAG AAGGTGTAAT CATCTATTCT GTGGGAGTGT TTGGCTCCAA TGTCACCCAG 
CTTGAGGAGA TCAGTGGGAG GCCCGAGATG GTTTTTTATG TTGAGAATTT TGACATTCTG 
CAGCGCATTG AAGATGATCT TGTTTTTGGA ATATGCAGCC CCCGTGAAGA ATGCAAGCGG 
ATTGAAGTTT TAGACGTTGT GTTTGTCATT GATAGCTCTG GCAGTATTGA CTATGATGAG 
TATAATATCA TGAAGGATTT TATGATTGGC TTAGTGAAAA AAGCTGATGT GGGCAAGAAT 
CAGGTCCGGT TTGGGGCTCT GAAGTATGCT GATGACCCAG AGGTGCTGTT TTATCTGGAT 
GACTTTGGCA CAAAACTGGA GGTAATTTCA GTGCTCCAGA ATGACCAAGC CATGGGTGGC 
AGTACTTATA CTGCTGAGGC ACTGGGCTTC TCAGACCACA TGTTCACTGA AGCCCGGGGC 
AGCCGCCTGA ACAAGGGGGT CCCCCAAGTC CTCATTGTGA TCACCGATGG GGAATCCCAT 
GATGCTGATA AACTCAATGC CACGGCAAAG GCCTTGCGGG ACAAAGGCAT TCTTGTCCTG 
GCTGTGGGGA TTGATGGTGC CAATCCCGTG GAGCTGTTAG CCATGGCAGG ATCAAGCGAC 
AAGTACTTCT TCGTGGAGAC TTTTGGAGGT CTGAAGGGAA TATTTTCAGA TGTGACAGCC 
AGTGTCTGCA ACTCTTCAAA AGTAGATTGT GAAATTGACA AAGTAGATCT TGTTTTCCTT 
ATGGATGGTT CAACTAGCAT TCAGCCAAAT GACTTCAAGA AAATGAAGGA ATTTCTGGCA 
TCTGTTGTTC AAGACTTTGA TGTCAGCCTC AACAGAGTGC GAATAGGAGC GGCCCAGTTT 
AGCGATACCT ATCACCCGGA GTTTCCACTG GGAACTTTCA TAGGTGAAAA AGAGATATCA 
TTTCAGATTG AAAACATCAA GCAGATCTTT GGAAACACAC ACATCGGTGC TGCACTCAGG 
GAGGTGGAAC ATTACTTCAG GCCAGACATG GGCAGCAGGA TAAATACAGG TACCCCACAG 
GTGCTGCTGG TCCTTACAGA TGGCCAGTCC CAAGACGAGG TGGCCCAGGC CGCGGAAGCC 
CTGAGACACA GAGGTATCGA CATCTACTCC GTGGGCATTG GGGATGTGGA TGACCAGCAG 
CTCATTCAGA TCACCGGGAC TGCAGAGAAA AAACTGACAG TGCACAACTT CGATGAACTG 
AAGAAGGTCA ATAAAAGGAT CGTTCGCAAC ATCTGTACCA CAGCGGGTGA AAGCAACTGT 
TTCGTGGATG TTGTGGTGGG ATTTGATGTC TCAACTCAGG AGAAAGGGCA GACTTTGCTT 
GAAGGTCAGC CTTGGATGGA AACCTACCTT CAAGACATCT TACGTGCCAT CAGCTCCCTC 
AATGGAGTAA GCTGTGAGGT GGGCACAGAG ACTCAGGTCA GTGTGGCTTT TCAAGTGACC 
AATGCCATGG AAAAATATTC TCCCAAGTTT GAGATCTACA GTGAAAACAT ACTGAATAGC 
TTGAAGGATA TAACAGTTAA AGGACCATCT CTTCTCAATG CAAACCTCTT GGATTCTCTA 
TGGGATACAT TTCAGAATAA ATCAGCTGCT CGAGGAAAGG TGGTCCTTTT ATTTTCAGAT 
GGATTGGATG ATGATGTTGA GAAACTTGAA CAAAAATCTG ATGAACTTAG AAAAGAAGGC 
CTGAATGCCC TCATAACTGT TGCTCTGGAT GGACCTGCTG ATTCAAGTGA CTTGGCTGAT 
CTTCCCTATA TTGAATTTGG GAAAGGATTT GAGTACAGGA CACAGCTCTC TATTGGCATG 
AGAGAACTTG GAAGCCGGCT GTCAAAGCAG CTGGTCAATG TTGCTGAAAG GACATGCTGC 
TGTTTGTTCT GCAAGTGCAT TGGAGGAGAT GGCACAATGG GAGATCCTGG ACCACCAGGG 
AAAAGGGGAC CTCCAGGTTT TAAAGGCAGT GAAGGCTACC TGGGAGAGGA GGGAATCGCT 
GGAGAAAGAG GAGCCCCTGG ACCAGTGGGA GAGCAAGGTA CTAAGGGATG CTATGGCACC 
AAAGGTCCTA AGGGAAACAG AGGACTAAAT GGACAGGAGG GAGAAGTTGG GGAAAATGGA 
ATTGACGGAT TAAACGGAGA ACAGGGTGAT AATGGTCTTC CTGGAAGAAA AGGAGAAAAG 
GGAGATGAGG GATCTCAGGG AAGCCCAGGG AAGAGAGGGA CTCCTGGTGA CCGTGGAGCA 
AAGGGCCTGC GAGGGGATCC CGGAGCTCCT GGAGTTGACA GTAGCATAGA AGGACCCACA 
GGCTTGAAAG GAGAACGTGG AAGACAAGGC AGAAGAGGCT GGCCAGGCCC CCCCGGGACA 
CCAGGCTCCA GAAGAAAGAC AGCAGCTCAT GGCAGAAGGG GACATACAGG CCCACAGGGA 
ACAGCAGGCA TCCCAGGACC AGATGGACTT GAAGGCTCCC TGGGACTTAA GGGCCCTCAG 
GGCCCAAGAG GAGAGGCTGG TGTGAAAGGA GAAAAAGGAG GTGTGGGAAG TAAAGGTCCC 
CAGGGGCCTC CAGGACCCGG AGGAGAGGCA GGGAATCAAG GCCGTTTGGG AAGCCAAGGA 
AATAAAGGAG AACCTGGAGA TCTGGGAGAA AAAGGAGCTG TTGGCTTTCC TGGTCCTCGT 
GGCTTGCAGG GCAATGATGG CAGTCCAGGT TATGGTAGTG TCGGACGCAA GGGAGCAAAG 
GGACAAGAAG GATTCCCTGG AGAAAGTGGA CCTAAGGGTG AGATTGGGGA CCCTGGTGGT 
CCAGGAGAGA CTGGGCTGAA GGGAGCTAGA GGCAAAATGA TATCTGCTGG GCTTCCAGGA 
GAGATGGGAT CCCCTGGGGA ACCAGGACCT CCTGGACGTA AGGGTGTGAA AGGAGCCAAA 
GGCTTGGCTT CATTTTCTAC ATGTGAGCTC ATTCAGTATG TGCGAGACCG CAGTCCTGGC 
AGGCATGGAA AACCGGAATG CCCAGTGCAC CCAACCGAGT TGGTGTTTGC CCTGGACCAC 
TCCCGGGATG TCACTGAGCA GGAATTTGAG CGGATGAAGG AGATGATGGC TTTCCTGGTG 
AGAGACATTA AGGTCCGGGA GAACAGCTGC CCCGTGGGAG CGCACATCGC CATCCTCTCC 
TATAACTCCC ACGCCAGGCA CCTTGTGCGC TTCTCAGACG CCTACAAGAA GAGTCAACTT 
CTCAGAGAAA TTGAAACTAT TCCTTATGAG AGATCCTCTG CCAGCAGGGA GATTGGCAGA 
GCAATGCGGT TTATTTCCAG GAATGTCTTC AAGCGGACGC TTCCGGGGGC ACACACGAGA 
AAAATCGCCA CATTTTTCAG CAGCGGTCAG TCCGCGGATG CCCACTCCAT CACCACGGCT 
GCCATGGAGT TCGGCGCGCT TGAAATCATT CCCGTGGTGA TCACTTTCAG CAACGTGCCC 
TCGGTCAGGC GCGCATTTGC GATTGACGAC ACTGGCACAT TTCAAGTAAT AGTGGTTCCC 
TCCGGGGCCG ACTACATACC AGCATTAGAG AGACTCCAGC GGTGCACTTT CTGCTATGAT 
GTGTGCAAGC CAGATGCTTC TTGTGACCAA GCCAGACCAC CCCCTGTGCA GTCTTACATG 
GATGCTGCTT TCCTTCTGGA TGCCTCCCGG AACATGGGAA GTGCTGAATT TGAAGACATA 
AGAGCCTTCC TTGGAGCACT ATTAGATCAC TTTGAAATCA CCCCAGAGCC GGAGACTTCT 
GTCACTGGAG ACCGGGTGGC CCTATTGAGC CATGCTCCCC CCGACTTCCT ACCCAACACT 
CAGAAGAGTC CAGTTAGAGC TGAGTTCAAT CTTACCACCT ACAGAAGTAA GCGCCTCATG 
AAGAGGCATG TGCACGAGTC AGTTAAACAA CTAAATGGAG ATGCTTTTAT TGGTCATGCC 
TTACAGTGGA CTCTGGACAA TGTATTTTTA AGTACACCCA ATCTGAGAAG AAACAAAGTC 
ATATTTGTGA TATCTGCTGG GGAAACCAGC CACTTAGATG GGGAAATCTT AAAGAAGGAA 
TCCTTGCGAG CCAAATGTCA GGGATATGCC TTATTTGTGT TTTCCCTTGG CCCTATTTGG 
GATGACAAGG AACTGGAGGA TCTCGCCAGC CACCCTTTGG ATCACCACCT GGTCCAGCTT 
GGCCGAATTC ATAAACCTGA CCACAGTTAT GGTGTGAAGT TTGTGAAGTC CTTTATAAAC 
TCAATCAGGC GTGCAATCAA CAAATATCCA CCAATAAACT TAAAAATAAA GTGCAACAGA 
CTTAACTCTA TAGATCCAAA GCAGCCCCCA CGACCATTCC GAAGCTTTGT TCCTGGACCA 
CTTAAAGCTA CCCTCAAAGA AGATGTATTA CAGAAGGCAA AATTCTTTCA AGATAAAAAA 
TATCTTTCAA GAGTAGCAAG AAGTGGCAGA GATGATGCTA TTCAAAATTT TATGAGAAGC 
ACCTCCCATA CCTTTAAGAA TGGAAGGATG ATAGAAAGTG CTCCCAAACA ACATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510725.1
CDS	208..7047
Translation	MEVSPRFEVEDFSGHNMMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD

Target ORF information:

RefSeq Version	XM_011512423.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 6 (COL6A6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512423.2

ORF Insert Sequence:

ATGGAAGTTT CCCCAAGATT TGAAGTTGAA GATTTTTCAG GTCATAATAT GATGTTGCTA 
ATTTTGTTCC TCGTGATAAT TTGTTCCCAT ATTTCTGTGA ACCAAGATTC CGGCCCTGAG 
TATGCAGATG TTGTGTTTTT GGTGGACAGC TCTGATCGCC TGGGATCCAA GTCCTTCCCA 
TTTGTGAAAA TGTTCATCAC CAAAATGATC AGCAGTCTCC CCATAGAGGC CGACAAATAC 
CGTGTGGCCC TGGCCCAGTA CAGTGATAAA CTTCACAGTG AATTCCACCT GAGCACCTTC 
AAAGGCAGGA GCCCCATGCT GAACCACCTA AGGAAGAACT TTGGATTCAT TGGCGGGTCC 
CTGCAGATAG GAAAGGCTCT TCAGGAGGCT CACAGGACTT ATTTCTCTGC ACCCGCAAAT 
GGGAGAGACA AGAAACAGTT TCCCCCAATT CTAGTGGTCC TGGCTTCATC TGAGTCTGAG 
GATAATGTGG AAGAGGCATC AAAGGCCCTG CGGAAAGACG GAGTGAAAAT CATCTCTGTA 
GGGGTGCAGA AAGCTTCTGA GGAAAACCTG AAGGCCATGG CCACGTCTCA GTTTCATTTC 
AACCTTCGGA CAGTCAGAGA CCTCAGCATG TTTTCCCAAA ACATGACACA CATCATCAAG 
GATGTAATAA AGTACAAGGA GGGAGCAGTT GATGACATCT TTGTAGAAGC TTGCCAAGGC 
CCTTCTATGG CCGATGTTGT GTTCCTATTG GATATGTCAA TCAATGGAAG TGAGGAGAAC 
TTTGACTATC TTAAAGGATT CTTGGAAGAA AGTGTATCTG CCCTTGACAT AAAGGAAAAT 
TGCATGAGGG TTGGCCTTGT GGCCTATAGC AATGAGACAA AAGTGATAAA TTCACTGAGC 
ATGGGCATAA ATAAGTCAGA GGTTCTCCAG CATATACAGA ACCTTTCTCC CCGAACTGGG 
AAGGCCTATA CTGGAGCTGC CATCAAAAAG CTCAGGAAGG AAGTTTTTAG TGCACGGAAT 
GGCAGTCGGA AGAATCAGGG GGTGCCCCAG ATTGCCGTGC TGGTGACCCA CCGAGATTCA 
GAAGACAACG TGACAAAAGC AGCTGTTAAC CTCCGACGGG AGGGTGTGAC CATCTTCACC 
CTGGGCATAG AGGGCGCCAG CGACACCCAG TTGGAAAAGA TAGCATCCCA CCCTGCTGAG 
CAGTATGTCT CCAAACTGAA GACCTTCGCT GACCTGGCTG CTCACAACCA GACATTTCTG 
AAGAAGCTGC GGAACCAAAT AACACACACA GTCTCTGTCT TTTCAGAGAG GACTGAAACG 
CTCAAATCTG GTTGTGTGGA CACTGAGGAA GCAGACATCT ATCTGCTTAT CGATGGCTCA 
GGGAGCACCC AGGCCACAGA TTTCCATGAA ATGAAGACGT TCCTGTCAGA GGTGGTAGGG 
ATGTTCAACA TTGCTCCCCA TAAGGTGCGG GTTGGGGCCG TTCAGTATGC TGACAGCTGG 
GACTTGGAAT TTGAGATCAA TAAATACTCC AACAAGCAGG ATTTGGGAAA GGCCATTGAG 
AATATCAGGC AGATGGGTGG GAATACAAAC ACAGGCGCAG CACTGAATTT CACACTGAGT 
CTGTTGCAAA AAGCAAAGAA GCAGCGAGGA AACAAAGTTC CATGCCACCT TGTTGTCCTG 
ACAAATGGCA TGTCCAAGGA TAGCATCTTG GAGCCTGCAA ACAGACTGAG AGAAGAGCAC 
ATCCGAGTTT ATGCTATCGG GATCAAGGAG GCCAACCAAA CACAGCTGAG AGAAATTGCA 
GGAGAGGAAA AGAGAGTGTA TTACGTGCAT GACTTTGATG CATTGAAAGA CATAAGAAAC 
CAAGTTGTTC AAGAAATCTG TACTGAAGAA GCTTGCAAAG AGATGAAAGC TGACATCATG 
TTTCTGGTGG ACAGTTCTGG AAGTATAGGA CCTGAAAACT TCAGCAAAAT GAAAACATTT 
ATGAAAAACC TGGTGAGCAA GTCTCAGATT GGACCAGATC GGGTGCAAAT TGGTGTAGTC 
CAGTTCAGCG ACATCAATAA GGAAGAGTTT CAGCTCAACA GATTCATGTC CCAAAGCGAC 
ATTTCAAATG CAATAGACCA AATGGCTCAC ATTGGACAAA CCACCCTGAC TGGTAGTGCC 
CTGAGCTTTG TGTCTCAGTA CTTCAGCCCC ACCAAGGGCG CCCGGCCCAA CATCAGAAAG 
TTTCTCATCC TCATCACGGA TGGTGAAGCT CAGGACATAG TAAAGGAACC AGCAGTAGTG 
CTTCGGCAAG AAGGTGTAAT CATCTATTCT GTGGGAGTGT TTGGCTCCAA TGTCACCCAG 
CTTGAGGAGA TCAGTGGGAG GCCCGAGATG GTTTTTTATG TTGAGAATTT TGACATTCTG 
CAGCGCATTG AAGATGATCT TGTTTTTGGA ATATGCAGCC CCCGTGAAGA ATGCAAGCGG 
ATTGAAGTTT TAGACGTTGT GTTTGTCATT GATAGCTCTG GCAGTATTGA CTATGATGAG 
TATAATATCA TGAAGGATTT TATGATTGGC TTAGTGAAAA AAGCTGATGT GGGCAAGAAT 
CAGGTCCGGT TTGGGGCTCT GAAGTATGCT GATGACCCAG AGGTGCTGTT TTATCTGGAT 
GACTTTGGCA CAAAACTGGA GGTAATTTCA GTGCTCCAGA ATGACCAAGC CATGGGTGGC 
AGTACTTATA CTGCTGAGGC ACTGGGCTTC TCAGACCACA TGTTCACTGA AGCCCGGGGC 
AGCCGCCTGA ACAAGGGGGT CCCCCAAGTC CTCATTGTGA TCACCGATGG GGAATCCCAT 
GATGCTGATA AACTCAATGC CACGGCAAAG GCCTTGCGGG ACAAAGGCAT TCTTGTCCTG 
GCTGTGGGGA TTGATGGTGC CAATCCCGTG GAGCTGTTAG CCATGGCAGG ATCAAGCGAC 
AAGTACTTCT TCGTGGAGAC TTTTGGAGGT CTGAAGGGAA TATTTTCAGA TGTGACAGCC 
AGTGTCTGCA ACTCTTCAAA AGTAGATTGT GAAATTGACA AAGTAGATCT TGTTTTCCTT 
ATGGATGGTT CAACTAGCAT TCAGCCAAAT GACTTCAAGA AAATGAAGGA ATTTCTGGCA 
TCTGTTGTTC AAGACTTTGA TGTCAGCCTC AACAGAGTGC GAATAGGAGC GGCCCAGTTT 
AGCGATACCT ATCACCCGGA GTTTCCACTG GGAACTTTCA TAGGTGAAAA AGAGATATCA 
TTTCAGATTG AAAACATCAA GCAGATCTTT GGAAACACAC ACATCGGTGC TGCACTCAGG 
GAGGTGGAAC ATTACTTCAG GCCAGACATG GGCAGCAGGA TAAATACAGG TACCCCACAG 
GTGCTGCTGG TCCTTACAGA TGGCCAGTCC CAAGACGAGG TGGCCCAGGC CGCGGAAGCC 
CTGAGACACA GAGGTATCGA CATCTACTCC GTGGGCATTG GGGATGTGGA TGACCAGCAG 
CTCATTCAGA TCACCGGGAC TGCAGAGAAA AAACTGACAG TGCACAACTT CGATGAACTG 
AAGAAGGTCA ATAAAAGGAT CGTTCGCAAC ATCTGTACCA CAGCGGGTGA AAGCAACTGT 
TTCGTGGATG TTGTGGTGGG ATTTGATGTC TCAACTCAGG AGAAAGGGCA GACTTTGCTT 
GAAGGTCAGC CTTGGATGGA AACCTACCTT CAAGACATCT TACGTGCCAT CAGCTCCCTC 
AATGGAGTAA GCTGTGAGGT GGGCACAGAG ACTCAGGTCA GTGTGGCTTT TCAAGTGACC 
AATGCCATGG AAAAATATTC TCCCAAGTTT GAGATCTACA GTGAAAACAT ACTGAATAGC 
TTGAAGGATA TAACAGTTAA AGGACCATCT CTTCTCAATG CAAACCTCTT GGATTCTCTA 
TGGGATACAT TTCAGAATAA ATCAGCTGCT CGAGGAAAGG TGGTCCTTTT ATTTTCAGAT 
GGATTGGATG ATGATGTTGA GAAACTTGAA CAAAAATCTG ATGAACTTAG AAAAGAAGGC 
CTGAATGCCC TCATAACTGT TGCTCTGGAT GGACCTGCTG ATTCAAGTGA CTTGGCTGAT 
CTTCCCTATA TTGAATTTGG GAAAGGATTT GAGTACAGGA CACAGCTCTC TATTGGCATG 
AGAGAACTTG GAAGCCGGCT GTCAAAGCAG CTGGTCAATG TTGCTGAAAG GACATGCTGC 
TGTTTGTTCT GCAAGTGCAT TGGAGGAGAT GGCACAATGG GAGATCCTGG ACCACCAGGG 
AAAAGGGGAC CTCCAGGTTT TAAAGGCAGT GAAGGCTACC TGGGAGAGGA GGGAATCGCT 
GGAGAAAGAG GAGCCCCTGG ACCAGTGGGA GAGCAAGGTA CTAAGGGATG CTATGGCACC 
AAAGGTCCTA AGGGAAACAG AGGACTAAAT GGACAGGAGG GAGAAGTTGG GGAAAATGGA 
ATTGACGGAT TAAACGGAGA ACAGGGTGAT AATGGTCTTC CTGGAAGAAA AGGAGAAAAG 
GGAGATGAGG GATCTCAGGG AAGCCCAGGG AAGAGAGGGA CTCCTGGTGA CCGTGGAGCA 
AAGGGCCTGC GAGGGGATCC CGGAGCTCCT GGAGTTGACA GTAGCATAGA AGGACCCACA 
GGCTTGAAAG GAGAACGTGG AAGACAAGGC AGAAGAGGCT GGCCAGGCCC CCCCGGGACA 
CCAGGCTCCA GAAGAAAGAC AGCAGCTCAT GGCAGAAGGG GACATACAGG CCCACAGGGA 
ACAGCAGGCA TCCCAGGACC AGATGGACTT GAAGGCTCCC TGGGACTTAA GGGCCCTCAG 
GGCCCAAGAG GAGAGGCTGG TGTGAAAGGA GAAAAAGGAG GTGTGGGAAG TAAAGGTCCC 
CAGGGGCCTC CAGGACCCGG AGGAGAGGCA GGGAATCAAG GCCGTTTGGG AAGCCAAGGA 
AATAAAGGAG AACCTGGAGA TCTGGGAGAA AAAGGAGCTG TTGGCTTTCC TGGTCCTCGT 
GGCTTGCAGG GCAATGATGG CAGTCCAGGT TATGGTAGTG TCGGACGCAA GGGAGCAAAG 
GGACAAGAAG GATTCCCTGG AGAAAGTGGA CCTAAGGGTG AGATTGGGGA CCCTGGTGGT 
CCAGGAGAGA CTGGGCTGAA GGGAGCTAGA GGCAAAATGA TATCTGCTGG GCTTCCAGGA 
GAGATGGGAT CCCCTGGGGA ACCAGGACCT CCTGGACGTA AGGGTGTGAA AGGAGCCAAA 
GGCTTGGCTT CATTTTCTAC ATGTGAGCTC ATTCAGTATG TGCGAGACCG CAGTCCTGGC 
AGGCATGGAA AACCGGAATG CCCAGTGCAC CCAACCGAGT TGGTGTTTGC CCTGGACCAC 
TCCCGGGATG TCACTGAGCA GGAATTTGAG CGGATGAAGG AGATGATGGC TTTCCTGGTG 
AGAGACATTA AGGTCCGGGA GAACAGCTGC CCCGTGGGAG CGCACATCGC CATCCTCTCC 
TATAACTCCC ACGCCAGGCA CCTTGTGCGC TTCTCAGACG CCTACAAGAA GAGTCAACTT 
CTCAGAGAAA TTGAAACTAT TCCTTATGAG AGATCCTCTG CCAGCAGGGA GATTGGCAGA 
GCAATGCGGT TTATTTCCAG GAATGTCTTC AAGCGGACGC TTCCGGGGGC ACACACGAGA 
AAAATCGCCA CATTTTTCAG CAGCGGTCAG TCCGCGGATG CCCACTCCAT CACCACGGCT 
GCCATGGAGT TCGGCGCGCT TGAAATCATT CCCGTGGTGA TCACTTTCAG CAACGTGCCC 
TCGGTCAGGC GCGCATTTGC GATTGACGAC ACTGGCACAT TTCAAGTAAT AGTGGTTCCC 
TCCGGGGCCG ACTACATACC AGCATTAGAG AGACTCCAGC GGTGCACTTT CTGCTATGAT 
GTGTGCAAGC CAGATGCTTC TTGTGACCAA GCCAGACCAC CCCCTGTGCA GTCTTACATG 
GATGCTGCTT TCCTTCTGGA TGCCTCCCGG AACATGGGAA GTGCTGAATT TGAAGACATA 
AGAGCCTTCC TTGGAGCACT ATTAGATCAC TTTGAAATCA CCCCAGAGCC GGAGACTTCT 
GTCACTGGAG ACCGGGTGGC CCTATTGAGC CATGCTCCCC CCGACTTCCT ACCCAACACT 
CAGAAGAGTC CAGTTAGAGC TGAGTTCAAT CTTACCACCT ACAGAAGTAA GCGCCTCATG 
AAGAGGCATG TGCACGAGTC AGTTAAACAA CTAAATGGAG ATGCTTTTAT TGGTCATGCC 
TTACAGTGGA CTCTGGACAA TGTATTTTTA AGTACACCCA ATCTGAGAAG AAACAAAGTC 
ATATTTGTGA TATCTGCTGG GGAAACCAGC CACTTAGATG GGGAAATCTT AAAGAAGGAA 
TCCTTGCGAG CCAAATGTCA GGGATATGCC TTATTTGTGT TTTCCCTTGG CCCTATTTGG 
GATGACAAGG AACTGGAGGA TCTCGCCAGC CACCCTTTGG ATCACCACCT GGTCCAGCTT 
GGCCGAATTC ATAAACCTGA CCACAGTTAT GGTGTGAAGT TTGTGAAGTC CTTTATAAAC 
TCAATCAGGC GTGCAATCAA CAAATATCCA CCAATAAACT TAAAAATAAA GTGCAACAGA 
CTTAACTCTA TAGATCCAAA GCAGCCCCCA CGACCATTCC GAAGCTTTGT TCCTGGACCA 
CTTAAAGCTA CCCTCAAAGA AGATGTATTA CAGAAGGCAA AATTCTTTCA AGATAAAAAA 
TATCTTTCAA GAGTAGCAAG AAGTGGCAGA GATGATGCTA TTCAAAATTT TATGAGAAGC 
ACCTCCCATA CCTTTAAGAA TGGAAGGATG ATAGAAAGTG CTCCCAAACA ACATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Journal of proteome research16(8)3083-3091(2017 Aug)
Naba A,Pearce OMT,Del Rosario A,Ma D,Ding H,Rajeeve V,Cutillas PR,Balkwill FR,Hynes RO

Identification of novel candidate variants including COL6A6 polymorphisms in early-onset atopic dermatitis using whole-exome sequencing.
BMC medical genetics18(1)8(2017 Jan)
Heo WI,Park KY,Jin T,Lee MK,Kim M,Choi EH,Kim HS,Bae JM,Moon NJ,Seo SJ

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

New COL6A6 variant detected by whole-exome sequencing is linked to break points in intron 4 and 3'-UTR, deleting exon 5 of RHO, and causing adRP.
Molecular vision21857-70(2015)
de Sousa Dias M,Hernan I,Del?s B,Pascual B,Borr?s E,Gamundi MJ,Ma?? B,Fern?ndez-San Jos? P,Ayuso C,Carballo M

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COL6A6 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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