Col6a6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col6a6
Entrez Gene ID	245026
Full Name	collagen, type VI, alpha 6
Synonyms	E330019B14,E330026B02Rik
General protein information	Preferred Names collagen, type VI, alpha 6 Names collagen alpha-6(VI) chain
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 F1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006511733.2	XP_006511796.1	collagen alpha-6(VI) chain isoform X2
XM_006511732.4	XP_006511795.1	collagen alpha-6(VI) chain isoform X1
XM_006511733.3	XP_006511796.1	collagen alpha-6(VI) chain isoform X2
NM_001102607.1	NP_001096077.1	collagen alpha-6(VI) chain isoform 1 precursor
XM_006511732.3	XP_006511795.1	collagen alpha-6(VI) chain isoform X1
NM_172927.3	NP_766515.2	collagen alpha-6(VI) chain isoform 2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL6A6	XP_003310057.1
Bos taurus (cattle)	COL6A6	XP_002685218.2
Gallus gallus (chicken)	COL6A6	XP_426008.4
Xenopus tropicalis (tropical clawed frog)	LOC100486063	XP_002942405.2
Canis lupus familiaris (dog)	COL6A6	XP_853279.3
Mus musculus (house mouse)	Col6a6	NP_001096077.1
	COL6A6	XP_001113364.2
Homo sapiens (human)	COL6A6	NP_001096078.1
Rattus norvegicus (Norway rat)	Col6a6	XP_236593.5
Danio rerio (zebrafish)	col6a6	XP_692457.6

Gene Ontology
Bibliography

Related articles in PubMed

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

Regulatory mechanisms of anthrax toxin receptor 1-dependent vascular and connective tissue homeostasis.
Besschetnova TY, Ichimura T, Katebi N, St Croix B, Bonventre JV, Olsen BR
Matrix biology : journal of the International Society for Matrix Biology4256-73(2015 Mar)

A mouse model for dominant collagen VI disorders: heterozygous deletion of Col6a3 Exon 16.
Pan TC, Zhang RZ, Arita M, Bogdanovich S, Adams SM, Gara SK, Wagener R, Khurana TS, Birk DE, Chu ML
The Journal of biological chemistry289(15)10293-307(2014 Apr)

Differential and restricted expression of novel collagen VI chains in mouse.
Gara SK, Grumati P, Squarzoni S, Sabatelli P, Urciuolo A, Bonaldo P, Paulsson M, Wagener R
Matrix biology : journal of the International Society for Matrix Biology30(4)248-57(2011 May)

Three novel collagen VI chains, alpha4(VI), alpha5(VI), and alpha6(VI).
Fitzgerald J, Rich C, Zhou FH, Hansen U
The Journal of biological chemistry283(29)20170-80(2008 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Immunoelectron microscopy of skeletal muscle localized the collagen VI alpha 6 chain to the reticular lamina of muscle fibers
Title: Differential and restricted expression of novel collagen VI chains in mouse.

discovery of three additional collagen VI chains doubles the collagen VI family and adds a layer of complexity to collagen VI assembly and function in the extracellular matrix
Title: Three novel collagen VI chains, alpha4(VI), alpha5(VI), and alpha6(VI).

analysis of collagen VI chains alpha4, alpha5, and alpha6with high homology to the alpha3 chain
Title: Three novel collagen VI chains with high homology to the alpha3 chain.

The following Col6a6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col6a6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu43165	XM_006511733.3 Latest version!	Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu04312	NM_172927.3 Latest version!	Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$692.30 $989.00
OMu43164	XM_006511732.4 Latest version!	Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu21016	NM_001102607.1 Latest version!	Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu43165	XM_006511733.2	Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu43164	XM_006511732.3	Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col6a6 ( XM_006511733.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511796.1 Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu43165
Clone ID Related Accession (Same CDS sequence)	XM_006511733.2 , XM_006511733.3
Accession Version	XM_006511733.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-6(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511733.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGG TTTTGTGCCT GACAATGATT TGTTTCCACG TGTGTGTGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGACGT GGTGTTTCTG GTGGACAGCT CCGATCACCT AGGGCTTAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAC AAGATGATCA GCAGCCTCCC CATAGAGGCC 
AACAAGTACC GCGTGGCCCT GGCCCAGTAC AGCGATGCTC TCCACAATGA GTTCCAGCTG 
GGCACCTTCA AGAACAGGAA CCCCATGCTG AACCACCTGA AGAAGAACTT CGGGTTCATC 
GGTGGCTCCC TGAAGATAGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAATG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCA 
GAGTCTGAGG ATGATGTGGA AGAGGCTGCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAAACCTGA AGGCGATGGC CACCTCTCAG 
TTCCATTTCA ATCTCAGGAC TGCCAGAGAC CTCAGCGTGT TTGCCCCAAA CATGACAGAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GGAATGGCAG ATGACATTAT TGTAGAAGCC 
TGCCAAGGCC CTTCTGTGGC TGATGTGGTG TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAGGAGGACC TAGATCATCT TAAAGCATTC CTGGGCGAAA GCATCTCTGC CCTGGACATA 
AAGGAAAATT GCATGAGGGT TGGCCTGGTG ACCTATAGCA ATGAGACAAG GGTGATCAGC 
TCTCTGAGCA CGGGTAACAA CAAGACAGAA GTCTTGCAGC GCATACAGGA TCTGTCCCCT 
CAAGTAGGGC AGGCCTACAC TGGAGCTGCC CTCAGAAAGA CTAGGAAGGA AATCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTCCCTCAGA TCGCTGTGCT GGTGACCCAC 
AGAGCATCAG AAGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
ATCTTTACCA TGGGCATAGA GGGGGCTAAC CCAGACGAGC TGGAGAAGAT CGCATCCCAC 
CCTGCGGAGC AGTTCACCTC CAAACTGGGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAACTGCG GAACCAAATC ACACACACGG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCTGC CTGTGTGGAC ACAGAGGAAG CCGATATCTA TCTACTCATT 
GATGGTTCAG GGAGCACCCA GCCCACAGAC TTCCATGAAA TGAAGACCTT CCTGTCAGAG 
GTGGTAGGCA TGTTCAACAT TGCTCCCCAC AAGGTGCGAG TAGGGGCCGT GCAGTACGCC 
GACACCTGGG ACTTGGAATT TGAGATCTCT AAGTATAGTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGAGA ATATCAGGCA GATGGGTGGG AATACCAACA CAGGGGCGGC TTTGAACTTC 
ACACTGAAGC TGTTGCAAAG AGCAAAGAAG GAACGAGGAA GCAAAGTGCC GTGTCACCTG 
GTGGTTCTGA CCAATGGCAT GTCTCGGGAC AGCGTCCTGG GGCCTGCCCA TAAGCTGAGA 
GAGGAAAACA TCAGAGTGCA TGCGATCGGT GTCAAGGAAG CCAACCAAAC GCAGCTTCGG 
GAGATAGCGG GAGAGGAAAA GCGAGTTTAC TACGTCCATG AGTTCGATGC CTTGAGGAAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA CATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATCGGAC CTGAAAACTT CAGCAAGATG 
AAGATGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGCGTGGTCC AGTTCAGCCA CGAAAACAAG GAGGAGTTTC AGCTCAACAC GTTCATGTCT 
CAAAGTGACA TCGCCAACGC CATTGACCGA ATGACTCACA TTGGAGAAAC AACCTTGACG 
GGCAGTGCCC TGACCTTTGT GTCTCAGTAC TTCAGTCCCG ATAAGGGGGC CAGGCCCAAT 
GTCAGGAAGT TCCTCATTCT TATCACGGAT GGTGAGGCTC AGGACATAGT AAGGGACCCA 
GCGATCGCCC TTCGAAAAGA AGGTGTGATT ATCTATTCTG TGGGAGTATT CGGCTCCAAT 
GTCACCCAGC TTGAGGAGAT CAGTGGAAAG CCAGAGATGG TTTTCTATGT TGAGAATTTT 
GACATTCTGC AGCATATCGA AGATGACCTC GTTCTGGGGA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTGGTG TTTGTCATCG ATAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCCCTG AAGTATGCTG ATGACCCCGA AGTGCTGTTT 
TACCTGGATG AACTAGGCAC GAAGCTGGAG GTAGTTTCAG TGCTCCAGAA TGACCATCCC 
ATGGGTGGAA ATACTTACAC CGCTGAGGCC CTCGCCTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACCGACGGG 
GAATCTCATG ACGCAGAGAA GCTCAACACC ACCGCCAAGG CCCTGAGAGA CAAAGGCATT 
CTCGTCCTGG CTGTGGGGAT TGCCGGTGCC AACAGCTGGG AGCTCTTGGC CATGGCAGGG 
TCAAGCGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGAAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTTGATTGTG AAATTGAAAA GGTTGACCTT 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGGG 
TTTTTGGTGT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGCGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACGTTCAC CGGGGAGAGG 
GAGATATCCA CCCAGATTGA GGGCATCCAG CAGATCTTTG GATACACCCA CATCGGAGAT 
GCTCTCAGGA AGGTGAAGTA TTACTTTCAG CCAGACATGG GCAGCAGGAT CAACGCAGGT 
ACCCCCCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGACGAGGT AGCTCAGGCC 
GCCGAGGAGC TGAGACACAA AGGTGTGGAC ATCTACTCGG TGGGCATCGG GGATGTGGAT 
GACCAGGAAT TGGTCCAGAT CACGGGGACC GCGGAGAAAA AACTGACCGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGGAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGCT TTGTGGATGT CGTGGTTGGA TTTGACATCT CAAGCCTGCA GAGAGGGCAG 
ACTCTGCTCG AAGGTCAGCC TTGGATGGGA TCCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAAGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ACGCTATGGA AAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTAAGCAGCC TGCAGGGTGT GACCGTGAAT GGCCCGTCTC GCCTCAACGC CAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAATAAG TCAGCTGCTC GTGGGAAGGT GGTCCTTCTA 
TTTTCAGATG GACTGGATGA TGGCATTGAG AAACTTGAAC AGAAGTCTGA TGAACTCAGG 
AAGGAAGCAT TGCATCATGG GATTAGAGGA CATAGACAAT GGAGAGCAGC TGTGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511796.1
CDS	382..4401
Translation	MLLVLCLTMICFHVCVNQDSGPEYADVVFLVDSSDHLGLKSFPLVKTFIHKMISSLPIEANKYRVALAQYSDALHNEFQLGTFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEAAKALREDGVKIISVGVQKASEENLKAMATSQFHFNLRTARDLSVFAPNMTEIIKDVTQYREGMADDIIVEACQGPSVADVVFLLDMAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNVTKAAVNLRREGVTIFTMGIEGANPDELEKIASHPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSRDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEICAEEACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITDGEAQDIVRDPAIALRKEGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQDEVAQAAEELRHKGVDIYSVGIGDVDDQELVQITGTAEKKLTVHNFDELKKVKKRIVRNICTSGGESNCFVDVVVGFDISSLQRGQTLLEGQPWMGSYLQDLLRAISSLNGVSCEVGTETQVSIAFQVTNAMERYPSKFEIYSENILSSLQGVTVNGPSRLNANLLSSLWDTFQNKSAARGKVVLLFSDGLDDGIEKLEQKSDELRKEALHHGIRGHRQWRAAVD

Target ORF information:

RefSeq Version	XM_006511733.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511733.3

ORF Insert Sequence:

ATGCTGCTGG TTTTGTGCCT GACAATGATT TGTTTCCACG TGTGTGTGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGACGT GGTGTTTCTG GTGGACAGCT CCGATCACCT AGGGCTTAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAC AAGATGATCA GCAGCCTCCC CATAGAGGCC 
AACAAGTACC GCGTGGCCCT GGCCCAGTAC AGCGATGCTC TCCACAATGA GTTCCAGCTG 
GGCACCTTCA AGAACAGGAA CCCCATGCTG AACCACCTGA AGAAGAACTT CGGGTTCATC 
GGTGGCTCCC TGAAGATAGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAATG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCA 
GAGTCTGAGG ATGATGTGGA AGAGGCTGCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAAACCTGA AGGCGATGGC CACCTCTCAG 
TTCCATTTCA ATCTCAGGAC TGCCAGAGAC CTCAGCGTGT TTGCCCCAAA CATGACAGAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GGAATGGCAG ATGACATTAT TGTAGAAGCC 
TGCCAAGGCC CTTCTGTGGC TGATGTGGTG TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAGGAGGACC TAGATCATCT TAAAGCATTC CTGGGCGAAA GCATCTCTGC CCTGGACATA 
AAGGAAAATT GCATGAGGGT TGGCCTGGTG ACCTATAGCA ATGAGACAAG GGTGATCAGC 
TCTCTGAGCA CGGGTAACAA CAAGACAGAA GTCTTGCAGC GCATACAGGA TCTGTCCCCT 
CAAGTAGGGC AGGCCTACAC TGGAGCTGCC CTCAGAAAGA CTAGGAAGGA AATCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTCCCTCAGA TCGCTGTGCT GGTGACCCAC 
AGAGCATCAG AAGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
ATCTTTACCA TGGGCATAGA GGGGGCTAAC CCAGACGAGC TGGAGAAGAT CGCATCCCAC 
CCTGCGGAGC AGTTCACCTC CAAACTGGGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAACTGCG GAACCAAATC ACACACACGG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCTGC CTGTGTGGAC ACAGAGGAAG CCGATATCTA TCTACTCATT 
GATGGTTCAG GGAGCACCCA GCCCACAGAC TTCCATGAAA TGAAGACCTT CCTGTCAGAG 
GTGGTAGGCA TGTTCAACAT TGCTCCCCAC AAGGTGCGAG TAGGGGCCGT GCAGTACGCC 
GACACCTGGG ACTTGGAATT TGAGATCTCT AAGTATAGTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGAGA ATATCAGGCA GATGGGTGGG AATACCAACA CAGGGGCGGC TTTGAACTTC 
ACACTGAAGC TGTTGCAAAG AGCAAAGAAG GAACGAGGAA GCAAAGTGCC GTGTCACCTG 
GTGGTTCTGA CCAATGGCAT GTCTCGGGAC AGCGTCCTGG GGCCTGCCCA TAAGCTGAGA 
GAGGAAAACA TCAGAGTGCA TGCGATCGGT GTCAAGGAAG CCAACCAAAC GCAGCTTCGG 
GAGATAGCGG GAGAGGAAAA GCGAGTTTAC TACGTCCATG AGTTCGATGC CTTGAGGAAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA CATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATCGGAC CTGAAAACTT CAGCAAGATG 
AAGATGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGCGTGGTCC AGTTCAGCCA CGAAAACAAG GAGGAGTTTC AGCTCAACAC GTTCATGTCT 
CAAAGTGACA TCGCCAACGC CATTGACCGA ATGACTCACA TTGGAGAAAC AACCTTGACG 
GGCAGTGCCC TGACCTTTGT GTCTCAGTAC TTCAGTCCCG ATAAGGGGGC CAGGCCCAAT 
GTCAGGAAGT TCCTCATTCT TATCACGGAT GGTGAGGCTC AGGACATAGT AAGGGACCCA 
GCGATCGCCC TTCGAAAAGA AGGTGTGATT ATCTATTCTG TGGGAGTATT CGGCTCCAAT 
GTCACCCAGC TTGAGGAGAT CAGTGGAAAG CCAGAGATGG TTTTCTATGT TGAGAATTTT 
GACATTCTGC AGCATATCGA AGATGACCTC GTTCTGGGGA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTGGTG TTTGTCATCG ATAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCCCTG AAGTATGCTG ATGACCCCGA AGTGCTGTTT 
TACCTGGATG AACTAGGCAC GAAGCTGGAG GTAGTTTCAG TGCTCCAGAA TGACCATCCC 
ATGGGTGGAA ATACTTACAC CGCTGAGGCC CTCGCCTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACCGACGGG 
GAATCTCATG ACGCAGAGAA GCTCAACACC ACCGCCAAGG CCCTGAGAGA CAAAGGCATT 
CTCGTCCTGG CTGTGGGGAT TGCCGGTGCC AACAGCTGGG AGCTCTTGGC CATGGCAGGG 
TCAAGCGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGAAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTTGATTGTG AAATTGAAAA GGTTGACCTT 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGGG 
TTTTTGGTGT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGCGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACGTTCAC CGGGGAGAGG 
GAGATATCCA CCCAGATTGA GGGCATCCAG CAGATCTTTG GATACACCCA CATCGGAGAT 
GCTCTCAGGA AGGTGAAGTA TTACTTTCAG CCAGACATGG GCAGCAGGAT CAACGCAGGT 
ACCCCCCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGACGAGGT AGCTCAGGCC 
GCCGAGGAGC TGAGACACAA AGGTGTGGAC ATCTACTCGG TGGGCATCGG GGATGTGGAT 
GACCAGGAAT TGGTCCAGAT CACGGGGACC GCGGAGAAAA AACTGACCGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGGAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGCT TTGTGGATGT CGTGGTTGGA TTTGACATCT CAAGCCTGCA GAGAGGGCAG 
ACTCTGCTCG AAGGTCAGCC TTGGATGGGA TCCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAAGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ACGCTATGGA AAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTAAGCAGCC TGCAGGGTGT GACCGTGAAT GGCCCGTCTC GCCTCAACGC CAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAATAAG TCAGCTGCTC GTGGGAAGGT GGTCCTTCTA 
TTTTCAGATG GACTGGATGA TGGCATTGAG AAACTTGAAC AGAAGTCTGA TGAACTCAGG 
AAGGAAGCAT TGCATCATGG GATTAGAGGA CATAGACAAT GGAGAGCAGC TGTGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a6 ( NM_172927.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766515.2 Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu04312
Clone ID Related Accession (Same CDS sequence)	NM_172927.3
Accession Version	NM_172927.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	collagen alpha-6(VI) chain isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK054356.1 and AC119951.12. On Aug 30, 2007 this sequence version replaced NM_172927.2. Transcript Variant: This variant (2) uses an alternate splice site, compared to variant 1, that results in an isoform (2) with a shorter and distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK054356.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCTGCTGG TTTTGTGCCT GACAATGATT TGTTTCCACG TGTGTGTGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGACGT GGTGTTTCTG GTGGACAGCT CCGATCACCT AGGGCTTAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAC AAGATGATCA GCAGCCTCCC CATAGAGGCC 
AACAAGTACC GCGTGGCCCT GGCCCAGTAC AGCGATGCTC TCCACAATGA GTTCCAGCTG 
GGCACCTTCA AGAACAGGAA CCCCATGCTG AACCACCTGA AGAAGAACTT CGGGTTCATC 
GGTGGCTCCC TGAAGATAGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAATG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCA 
GAGTCTGAGG ATGATGTGGA AGAGGCTGCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAAACCTGA AGGCGATGGC CACCTCTCAG 
TTCCATTTCA ATCTCAGGAC TGCCAGAGAC CTCAGCGTGT TTGCCCCAAA CATGACAGAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GGAATGGCAG ATGACATTAT TGTAGAAGCC 
TGCCAAGGCC CTTCTGTGGC TGATGTGGTG TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAGGAGGACC TAGATCATCT TAAAGCATTC CTGGGCGAAA GCATCTCTGC CCTGGACATA 
AAGGAAAATT GCATGAGGGT TGGCCTGGTG ACCTATAGCA ATGAGACAAG GGTGATCAGC 
TCTCTGAGCA CGGGTAACAA CAAGACAGAA GTCTTGCAGC GCATACAGGA TCTGTCCCCT 
CAAGTAGGGC AGGCCTACAC TGGAGCTGCC CTCAGAAAGA CTAGGAAGGA AATCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTCCCTCAGA TCGCTGTGCT GGTGACCCAC 
AGAGCATCAG AAGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
ATCTTTACCA TGGGCATAGA GGGGGCTAAC CCAGACGAGC TGGAGAAGAT CGCATCCCAC 
CCTGCGGAGC AGTTCACCTC CAAACTGGGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAACTGCG GAACCAAATC ACACACACGG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCTGC CTGTGTGGAC ACAGAGGAAG CCGATATCTA TCTACTCATT 
GATGGTTCAG GGAGCACCCA GCCCACAGAC TTCCATGAAA TGAAGACCTT CCTGTCAGAG 
GTGGTAGGCA TGTTCAACAT TGCTCCCCAC AAGGTGCGAG TAGGGGCCGT GCAGTACGCC 
GACACCTGGG ACTTGGAATT TGAGATCTCT AAGTATAGTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGAGA ATATCAGGCA GATGGGTGGG AATACCAACA CAGGGGCGGC TTTGAACTTC 
ACACTGAAGC TGTTGCAAAG AGCAAAGAAG GAACGAGGAA GCAAAGTGCC GTGTCACCTG 
GTGGTTCTGA CCAATGGCAT GTCTCGGGAC AGCGTCCTGG GGCCTGCCCA TAAGCTGAGA 
GAGGAAAACA TCAGAGTGCA TGCGATCGGT GTCAAGGAAG CCAACCAAAC GCAGCTTCGG 
GAGATAGCGG GAGAGGAAAA GCGAGTTTAC TACGTCCATG AGTTCGATGC CTTGAGGAAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA CATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATCGGAC CTGAAAACTT CAGCAAGATG 
AAGATGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGCGTGGTCC AGTTCAGCCA CGAAAACAAG GAGGAGTTTC AGCTCAACAC GTTCATGTCT 
CAAAGTGACA TCGCCAACGC CATTGACCGA ATGACTCACA TTGGAGAAAC AACCTTGACG 
GGCAGTGCCC TGACCTTTGT GTCTCAGTAC TTCAGTCCCG ATAAGGGGGC CAGGCCCAAT 
GTCAGGAAGT TCCTCATTCT TATCACGGAT GGTGAGGCTC AGGACATAGT AAGGGACCCA 
GCGATCGCCC TTCGAAAAGA AGGTGTGATT ATCTATTCTG TGGGAGTATT CGGCTCCAAT 
GTCACCCAGC TTGAGGAGAT CAGTGGAAAG CCAGAGATGG TTTTCTATGT TGAGAATTTT 
GACATTCTGC AGCATATCGA AGATGACCTC GTTCTGGGGA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTGGTG TTTGTCATCG ATAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCCCTG AAGTATGCTG ATGACCCCGA AGTGCTGTTT 
TACCTGGATG AACTAGGCAC GAAGCTGGAG GTAGTTTCAG TGCTCCAGAA TGACCATCCC 
ATGGGTGGAA ATACTTACAC CGCTGAGGCC CTCGCCTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACCGACGGG 
GAATCTCATG ACGCAGAGAA GCTCAACACC ACCGCCAAGG CCCTGAGAGA CAAAGGCATT 
CTCGTCCTGG CTGTGGGGAT TGCCGGTGCC AACAGCTGGG AGCTCTTGGC CATGGCAGGG 
TCAAGCGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGAAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTTGATTGTG AAATTGAAAA GGTTGACCTT 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGGG 
TTTTTGGTGT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGCGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACGTTCAC CGGGGAGAGG 
GAGATATCCA CCCAGATTGA GGGCATCCAG CAGATCTTTG GATACACCCA CATCGGAGAT 
GCTCTCAGGA AGGTGAAGTA TTACTTTCAG CCAGACATGG GCAGCAGGAT CAACGCAGGT 
ACCCCCCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGACGAGGT AGCTCAGGCC 
GCCGAGGAGC TGAGACACAA AGGTGTGGAC ATCTACTCGG TGGGCATCGG GGATGTGGAT 
GACCAGGAAT TGGTCCAGAT CACGGGGACC GCGGAGAAAA AACTGACCGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGGAACA TCTGTACCTC AGGTGGTGAG 
AGCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766515.2
CDS	256..3804
Translation	MLLVLCLTMICFHVCVNQDSGPEYADVVFLVDSSDHLGLKSFPLVKTFIHKMISSLPIEANKYRVALAQYSDALHNEFQLGTFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEAAKALREDGVKIISVGVQKASEENLKAMATSQFHFNLRTARDLSVFAPNMTEIIKDVTQYREGMADDIIVEACQGPSVADVVFLLDMAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNVTKAAVNLRREGVTIFTMGIEGANPDELEKIASHPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSRDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEICAEEACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITDGEAQDIVRDPAIALRKEGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQDEVAQAAEELRHKGVDIYSVGIGDVDDQELVQITGTAEKKLTVHNFDELKKVKKRIVRNICTSGGESS

Target ORF information:

RefSeq Version	NM_172927.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172927.3

ORF Insert Sequence:

ATGCTGCTGG TTTTGTGCCT GACAATGATT TGTTTCCACG TGTGTGTGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGACGT GGTGTTTCTG GTGGACAGCT CCGATCACCT AGGGCTTAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAC AAGATGATCA GCAGCCTCCC CATAGAGGCC 
AACAAGTACC GCGTGGCCCT GGCCCAGTAC AGCGATGCTC TCCACAATGA GTTCCAGCTG 
GGCACCTTCA AGAACAGGAA CCCCATGCTG AACCACCTGA AGAAGAACTT CGGGTTCATC 
GGTGGCTCCC TGAAGATAGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAATG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCA 
GAGTCTGAGG ATGATGTGGA AGAGGCTGCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAAACCTGA AGGCGATGGC CACCTCTCAG 
TTCCATTTCA ATCTCAGGAC TGCCAGAGAC CTCAGCGTGT TTGCCCCAAA CATGACAGAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GGAATGGCAG ATGACATTAT TGTAGAAGCC 
TGCCAAGGCC CTTCTGTGGC TGATGTGGTG TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAGGAGGACC TAGATCATCT TAAAGCATTC CTGGGCGAAA GCATCTCTGC CCTGGACATA 
AAGGAAAATT GCATGAGGGT TGGCCTGGTG ACCTATAGCA ATGAGACAAG GGTGATCAGC 
TCTCTGAGCA CGGGTAACAA CAAGACAGAA GTCTTGCAGC GCATACAGGA TCTGTCCCCT 
CAAGTAGGGC AGGCCTACAC TGGAGCTGCC CTCAGAAAGA CTAGGAAGGA AATCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTCCCTCAGA TCGCTGTGCT GGTGACCCAC 
AGAGCATCAG AAGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
ATCTTTACCA TGGGCATAGA GGGGGCTAAC CCAGACGAGC TGGAGAAGAT CGCATCCCAC 
CCTGCGGAGC AGTTCACCTC CAAACTGGGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAACTGCG GAACCAAATC ACACACACGG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCTGC CTGTGTGGAC ACAGAGGAAG CCGATATCTA TCTACTCATT 
GATGGTTCAG GGAGCACCCA GCCCACAGAC TTCCATGAAA TGAAGACCTT CCTGTCAGAG 
GTGGTAGGCA TGTTCAACAT TGCTCCCCAC AAGGTGCGAG TAGGGGCCGT GCAGTACGCC 
GACACCTGGG ACTTGGAATT TGAGATCTCT AAGTATAGTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGAGA ATATCAGGCA GATGGGTGGG AATACCAACA CAGGGGCGGC TTTGAACTTC 
ACACTGAAGC TGTTGCAAAG AGCAAAGAAG GAACGAGGAA GCAAAGTGCC GTGTCACCTG 
GTGGTTCTGA CCAATGGCAT GTCTCGGGAC AGCGTCCTGG GGCCTGCCCA TAAGCTGAGA 
GAGGAAAACA TCAGAGTGCA TGCGATCGGT GTCAAGGAAG CCAACCAAAC GCAGCTTCGG 
GAGATAGCGG GAGAGGAAAA GCGAGTTTAC TACGTCCATG AGTTCGATGC CTTGAGGAAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA CATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATCGGAC CTGAAAACTT CAGCAAGATG 
AAGATGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGCGTGGTCC AGTTCAGCCA CGAAAACAAG GAGGAGTTTC AGCTCAACAC GTTCATGTCT 
CAAAGTGACA TCGCCAACGC CATTGACCGA ATGACTCACA TTGGAGAAAC AACCTTGACG 
GGCAGTGCCC TGACCTTTGT GTCTCAGTAC TTCAGTCCCG ATAAGGGGGC CAGGCCCAAT 
GTCAGGAAGT TCCTCATTCT TATCACGGAT GGTGAGGCTC AGGACATAGT AAGGGACCCA 
GCGATCGCCC TTCGAAAAGA AGGTGTGATT ATCTATTCTG TGGGAGTATT CGGCTCCAAT 
GTCACCCAGC TTGAGGAGAT CAGTGGAAAG CCAGAGATGG TTTTCTATGT TGAGAATTTT 
GACATTCTGC AGCATATCGA AGATGACCTC GTTCTGGGGA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTGGTG TTTGTCATCG ATAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCCCTG AAGTATGCTG ATGACCCCGA AGTGCTGTTT 
TACCTGGATG AACTAGGCAC GAAGCTGGAG GTAGTTTCAG TGCTCCAGAA TGACCATCCC 
ATGGGTGGAA ATACTTACAC CGCTGAGGCC CTCGCCTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACCGACGGG 
GAATCTCATG ACGCAGAGAA GCTCAACACC ACCGCCAAGG CCCTGAGAGA CAAAGGCATT 
CTCGTCCTGG CTGTGGGGAT TGCCGGTGCC AACAGCTGGG AGCTCTTGGC CATGGCAGGG 
TCAAGCGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGAAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTTGATTGTG AAATTGAAAA GGTTGACCTT 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGGG 
TTTTTGGTGT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGCGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACGTTCAC CGGGGAGAGG 
GAGATATCCA CCCAGATTGA GGGCATCCAG CAGATCTTTG GATACACCCA CATCGGAGAT 
GCTCTCAGGA AGGTGAAGTA TTACTTTCAG CCAGACATGG GCAGCAGGAT CAACGCAGGT 
ACCCCCCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGACGAGGT AGCTCAGGCC 
GCCGAGGAGC TGAGACACAA AGGTGTGGAC ATCTACTCGG TGGGCATCGG GGATGTGGAT 
GACCAGGAAT TGGTCCAGAT CACGGGGACC GCGGAGAAAA AACTGACCGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGGAACA TCTGTACCTC AGGTGGTGAG 
AGCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a6 ( XM_006511732.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511795.1 Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant X1, mRNA.

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CloneID	OMu43164
Clone ID Related Accession (Same CDS sequence)	XM_006511732.4 , XM_006511732.3
Accession Version	XM_006511732.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-6(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511732.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGG TTTTGTGCCT GACAATGATT TGTTTCCACG TGTGTGTGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGACGT GGTGTTTCTG GTGGACAGCT CCGATCACCT AGGGCTTAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAC AAGATGATCA GCAGCCTCCC CATAGAGGCC 
AACAAGTACC GCGTGGCCCT GGCCCAGTAC AGCGATGCTC TCCACAATGA GTTCCAGCTG 
GGCACCTTCA AGAACAGGAA CCCCATGCTG AACCACCTGA AGAAGAACTT CGGGTTCATC 
GGTGGCTCCC TGAAGATAGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAATG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCA 
GAGTCTGAGG ATGATGTGGA AGAGGCTGCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAAACCTGA AGGCGATGGC CACCTCTCAG 
TTCCATTTCA ATCTCAGGAC TGCCAGAGAC CTCAGCGTGT TTGCCCCAAA CATGACAGAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GGAATGGCAG ATGACATTAT TGTAGAAGCC 
TGCCAAGGCC CTTCTGTGGC TGATGTGGTG TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAGGAGGACC TAGATCATCT TAAAGCATTC CTGGGCGAAA GCATCTCTGC CCTGGACATA 
AAGGAAAATT GCATGAGGGT TGGCCTGGTG ACCTATAGCA ATGAGACAAG GGTGATCAGC 
TCTCTGAGCA CGGGTAACAA CAAGACAGAA GTCTTGCAGC GCATACAGGA TCTGTCCCCT 
CAAGTAGGGC AGGCCTACAC TGGAGCTGCC CTCAGAAAGA CTAGGAAGGA AATCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTCCCTCAGA TCGCTGTGCT GGTGACCCAC 
AGAGCATCAG AAGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
ATCTTTACCA TGGGCATAGA GGGGGCTAAC CCAGACGAGC TGGAGAAGAT CGCATCCCAC 
CCTGCGGAGC AGTTCACCTC CAAACTGGGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAACTGCG GAACCAAATC ACACACACGG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCTGC CTGTGTGGAC ACAGAGGAAG CCGATATCTA TCTACTCATT 
GATGGTTCAG GGAGCACCCA GCCCACAGAC TTCCATGAAA TGAAGACCTT CCTGTCAGAG 
GTGGTAGGCA TGTTCAACAT TGCTCCCCAC AAGGTGCGAG TAGGGGCCGT GCAGTACGCC 
GACACCTGGG ACTTGGAATT TGAGATCTCT AAGTATAGTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGAGA ATATCAGGCA GATGGGTGGG AATACCAACA CAGGGGCGGC TTTGAACTTC 
ACACTGAAGC TGTTGCAAAG AGCAAAGAAG GAACGAGGAA GCAAAGTGCC GTGTCACCTG 
GTGGTTCTGA CCAATGGCAT GTCTCGGGAC AGCGTCCTGG GGCCTGCCCA TAAGCTGAGA 
GAGGAAAACA TCAGAGTGCA TGCGATCGGT GTCAAGGAAG CCAACCAAAC GCAGCTTCGG 
GAGATAGCGG GAGAGGAAAA GCGAGTTTAC TACGTCCATG AGTTCGATGC CTTGAGGAAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA CATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATCGGAC CTGAAAACTT CAGCAAGATG 
AAGATGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGCGTGGTCC AGTTCAGCCA CGAAAACAAG GAGGAGTTTC AGCTCAACAC GTTCATGTCT 
CAAAGTGACA TCGCCAACGC CATTGACCGA ATGACTCACA TTGGAGAAAC AACCTTGACG 
GGCAGTGCCC TGACCTTTGT GTCTCAGTAC TTCAGTCCCG ATAAGGGGGC CAGGCCCAAT 
GTCAGGAAGT TCCTCATTCT TATCACGGAT GGTGAGGCTC AGGACATAGT AAGGGACCCA 
GCGATCGCCC TTCGAAAAGA AGGTGTGATT ATCTATTCTG TGGGAGTATT CGGCTCCAAT 
GTCACCCAGC TTGAGGAGAT CAGTGGAAAG CCAGAGATGG TTTTCTATGT TGAGAATTTT 
GACATTCTGC AGCATATCGA AGATGACCTC GTTCTGGGGA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTGGTG TTTGTCATCG ATAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCCCTG AAGTATGCTG ATGACCCCGA AGTGCTGTTT 
TACCTGGATG AACTAGGCAC GAAGCTGGAG GTAGTTTCAG TGCTCCAGAA TGACCATCCC 
ATGGGTGGAA ATACTTACAC CGCTGAGGCC CTCGCCTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACCGACGGG 
GAATCTCATG ACGCAGAGAA GCTCAACACC ACCGCCAAGG CCCTGAGAGA CAAAGGCATT 
CTCGTCCTGG CTGTGGGGAT TGCCGGTGCC AACAGCTGGG AGCTCTTGGC CATGGCAGGG 
TCAAGCGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGAAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTTGATTGTG AAATTGAAAA GGTTGACCTT 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGGG 
TTTTTGGTGT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGCGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACGTTCAC CGGGGAGAGG 
GAGATATCCA CCCAGATTGA GGGCATCCAG CAGATCTTTG GATACACCCA CATCGGAGAT 
GCTCTCAGGA AGGTGAAGTA TTACTTTCAG CCAGACATGG GCAGCAGGAT CAACGCAGGT 
ACCCCCCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGACGAGGT AGCTCAGGCC 
GCCGAGGAGC TGAGACACAA AGGTGTGGAC ATCTACTCGG TGGGCATCGG GGATGTGGAT 
GACCAGGAAT TGGTCCAGAT CACGGGGACC GCGGAGAAAA AACTGACCGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGGAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGCT TTGTGGATGT CGTGGTTGGA TTTGACATCT CAAGCCTGCA GAGAGGGCAG 
ACTCTGCTCG AAGGTCAGCC TTGGATGGGA TCCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAAGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ACGCTATGGA AAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTAAGCAGCC TGCAGGGTGT GACCGTGAAT GGCCCGTCTC GCCTCAACGC CAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAATAAG TCAGCTGCTC GTGGGAAGGT GGTCCTTCTA 
TTTTCAGATG GACTGGATGA TGGCATTGAG AAACTTGAAC AGAAGTCTGA TGAACTCAGG 
AAGGAAGGCC TGAATGCCCT CATAACCATT GCTGTGGATG GAGCTGCCGA TTCCAGTGAC 
CTGGCGGACC TCCTCTACAT TGAATTTGGG AAAGGATTTG AATACAGGAC ACAGTTCACA 
ATTGGAATGA GGAACCTTGG GAGTCAGCTG TCAAGGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTCTGCTCTG CAAGTGCACA GGAGGGGACG GCGCCATGGG GGATCCAGGC 
TCAGCAGGAA AAAAGGGACC CCCAGGATTC AAAGGCAGTG ATGGCTACCT CGGAGAGGAG 
GGCATTGCTG GAGAAAGAGG AGCCTCTGGG CCAATGGGAG AGCAAGGCAC GAAGGGATGC 
TTCGGTGCCA AAGGGCCTAA GGGAACCAGA GGACTCAGTG GAGAAGAGGG TGAAGTCGGG 
GAAGATGGGC TTGATGGATT AGATGGAGAA CAGGGTGACC ATGGGATTCC TGGAAGAAGA 
GGAGAAAAGG GTGACGAGGG ATCTCAGGGA AACCCAGGCA GGAGAGGGGC TGCCGGTGAC 
CGTGGAGCAA AGGGACTGCG AGGAGATCCG GGAACTCCTG GACGTGACAG TAGCATACAA 
GGACCCAAGG GCTTGAAAGG AGACCTTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGAACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACACATAGGC 
CCACAGGGAA ATCCAGGAAC CCCGGGCCCA GATGGACTTG CAGGTTCGCC AGGACTTAGA 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAAG TCTGGGAATG 
AAAGGTCCTC AGGGGCCTCC AGGACCCGGA GGACAAGCTG GGAGCCAAGG CCATTTGGGA 
AGCCAAGGAA ACAAAGGAGA ACCTGGAGAC CTGGGAGAAA AGGGAGCTGC TGGCTTTCCA 
GGTCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCGGGAT ATGGTAGCAT TGGCCGCAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC TGAAGGGTGA TATTGGGGAC 
CCTGGTGATC CAGGAGAAGC TGGTCCCAAG GGAGCCAGAG GCAAAACGGT ATCTGCTGGG 
ATTCCAGGAG AGCCGGGGTC CCCCGGGGAG CCAGGACCCC CTGGACGGAA GGGTGTGAAA 
GGGGCCCGAG GACTGGCTTC GTTTTCCACA TGTGACCTCA TTCAGTACGT GCGGGACCAC 
AGTCCTGGCA GACATGGAAA ACCTGAGTGC CCAGTGCATC CAACTGAGTT GGTGTTTGTC 
CTGGACCAGT CCCGGGATGT CACAGAGCAG GACTTTGAGC GAATGAAGGG GATGATGGTC 
TCCCTAGTTA GGGATGTCAA GATGCCTACA GGAAGGACCA GCTGCTCACG GCGATTAAAG 
CTCTTCCTTA TGAGAGATCC TCCGACAGCA GGGAGATTGG CAAAGCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511795.1
CDS	382..5832
Translation	MLLVLCLTMICFHVCVNQDSGPEYADVVFLVDSSDHLGLKSFPLVKTFIHKMISSLPIEANKYRVALAQYSDALHNEFQLGTFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEAAKALREDGVKIISVGVQKASEENLKAMATSQFHFNLRTARDLSVFAPNMTEIIKDVTQYREGMADDIIVEACQGPSVADVVFLLDMAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNVTKAAVNLRREGVTIFTMGIEGANPDELEKIASHPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSRDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEICAEEACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITDGEAQDIVRDPAIALRKEGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQDEVAQAAEELRHKGVDIYSVGIGDVDDQELVQITGTAEKKLTVHNFDELKKVKKRIVRNICTSGGESNCFVDVVVGFDISSLQRGQTLLEGQPWMGSYLQDLLRAISSLNGVSCEVGTETQVSIAFQVTNAMERYPSKFEIYSENILSSLQGVTVNGPSRLNANLLSSLWDTFQNKSAARGKVVLLFSDGLDDGIEKLEQKSDELRKEGLNALITIAVDGAADSSDLADLLYIEFGKGFEYRTQFTIGMRNLGSQLSRQLINVAERTCCCLLCKCTGGDGAMGDPGSAGKKGPPGFKGSDGYLGEEGIAGERGASGPMGEQGTKGCFGAKGPKGTRGLSGEEGEVGEDGLDGLDGEQGDHGIPGRRGEKGDEGSQGNPGRRGAAGDRGAKGLRGDPGTPGRDSSIQGPKGLKGDLGRQGRRGWPGSPGTPGSRRKMVVHGRRGHIGPQGNPGTPGPDGLAGSPGLRGPQGPRGEVGEKGEKGSLGMKGPQGPPGPGGQAGSQGHLGSQGNKGEPGDLGEKGAAGFPGPRGLQGDDGSPGYGSIGRKGTKGQEGFPGESGLKGDIGDPGDPGEAGPKGARGKTVSAGIPGEPGSPGEPGPPGRKGVKGARGLASFSTCDLIQYVRDHSPGRHGKPECPVHPTELVFVLDQSRDVTEQDFERMKGMMVSLVRDVKMPTGRTSCSRRLKLFLMRDPPTAGRLAKP

Target ORF information:

RefSeq Version	XM_006511732.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511732.4

ORF Insert Sequence:

ATGCTGCTGG TTTTGTGCCT GACAATGATT TGTTTCCACG TGTGTGTGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGACGT GGTGTTTCTG GTGGACAGCT CCGATCACCT AGGGCTTAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAC AAGATGATCA GCAGCCTCCC CATAGAGGCC 
AACAAGTACC GCGTGGCCCT GGCCCAGTAC AGCGATGCTC TCCACAATGA GTTCCAGCTG 
GGCACCTTCA AGAACAGGAA CCCCATGCTG AACCACCTGA AGAAGAACTT CGGGTTCATC 
GGTGGCTCCC TGAAGATAGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAATG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCA 
GAGTCTGAGG ATGATGTGGA AGAGGCTGCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAAACCTGA AGGCGATGGC CACCTCTCAG 
TTCCATTTCA ATCTCAGGAC TGCCAGAGAC CTCAGCGTGT TTGCCCCAAA CATGACAGAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GGAATGGCAG ATGACATTAT TGTAGAAGCC 
TGCCAAGGCC CTTCTGTGGC TGATGTGGTG TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAGGAGGACC TAGATCATCT TAAAGCATTC CTGGGCGAAA GCATCTCTGC CCTGGACATA 
AAGGAAAATT GCATGAGGGT TGGCCTGGTG ACCTATAGCA ATGAGACAAG GGTGATCAGC 
TCTCTGAGCA CGGGTAACAA CAAGACAGAA GTCTTGCAGC GCATACAGGA TCTGTCCCCT 
CAAGTAGGGC AGGCCTACAC TGGAGCTGCC CTCAGAAAGA CTAGGAAGGA AATCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTCCCTCAGA TCGCTGTGCT GGTGACCCAC 
AGAGCATCAG AAGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
ATCTTTACCA TGGGCATAGA GGGGGCTAAC CCAGACGAGC TGGAGAAGAT CGCATCCCAC 
CCTGCGGAGC AGTTCACCTC CAAACTGGGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAACTGCG GAACCAAATC ACACACACGG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCTGC CTGTGTGGAC ACAGAGGAAG CCGATATCTA TCTACTCATT 
GATGGTTCAG GGAGCACCCA GCCCACAGAC TTCCATGAAA TGAAGACCTT CCTGTCAGAG 
GTGGTAGGCA TGTTCAACAT TGCTCCCCAC AAGGTGCGAG TAGGGGCCGT GCAGTACGCC 
GACACCTGGG ACTTGGAATT TGAGATCTCT AAGTATAGTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGAGA ATATCAGGCA GATGGGTGGG AATACCAACA CAGGGGCGGC TTTGAACTTC 
ACACTGAAGC TGTTGCAAAG AGCAAAGAAG GAACGAGGAA GCAAAGTGCC GTGTCACCTG 
GTGGTTCTGA CCAATGGCAT GTCTCGGGAC AGCGTCCTGG GGCCTGCCCA TAAGCTGAGA 
GAGGAAAACA TCAGAGTGCA TGCGATCGGT GTCAAGGAAG CCAACCAAAC GCAGCTTCGG 
GAGATAGCGG GAGAGGAAAA GCGAGTTTAC TACGTCCATG AGTTCGATGC CTTGAGGAAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA CATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATCGGAC CTGAAAACTT CAGCAAGATG 
AAGATGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGCGTGGTCC AGTTCAGCCA CGAAAACAAG GAGGAGTTTC AGCTCAACAC GTTCATGTCT 
CAAAGTGACA TCGCCAACGC CATTGACCGA ATGACTCACA TTGGAGAAAC AACCTTGACG 
GGCAGTGCCC TGACCTTTGT GTCTCAGTAC TTCAGTCCCG ATAAGGGGGC CAGGCCCAAT 
GTCAGGAAGT TCCTCATTCT TATCACGGAT GGTGAGGCTC AGGACATAGT AAGGGACCCA 
GCGATCGCCC TTCGAAAAGA AGGTGTGATT ATCTATTCTG TGGGAGTATT CGGCTCCAAT 
GTCACCCAGC TTGAGGAGAT CAGTGGAAAG CCAGAGATGG TTTTCTATGT TGAGAATTTT 
GACATTCTGC AGCATATCGA AGATGACCTC GTTCTGGGGA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTGGTG TTTGTCATCG ATAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCCCTG AAGTATGCTG ATGACCCCGA AGTGCTGTTT 
TACCTGGATG AACTAGGCAC GAAGCTGGAG GTAGTTTCAG TGCTCCAGAA TGACCATCCC 
ATGGGTGGAA ATACTTACAC CGCTGAGGCC CTCGCCTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACCGACGGG 
GAATCTCATG ACGCAGAGAA GCTCAACACC ACCGCCAAGG CCCTGAGAGA CAAAGGCATT 
CTCGTCCTGG CTGTGGGGAT TGCCGGTGCC AACAGCTGGG AGCTCTTGGC CATGGCAGGG 
TCAAGCGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGAAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTTGATTGTG AAATTGAAAA GGTTGACCTT 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGGG 
TTTTTGGTGT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGCGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACGTTCAC CGGGGAGAGG 
GAGATATCCA CCCAGATTGA GGGCATCCAG CAGATCTTTG GATACACCCA CATCGGAGAT 
GCTCTCAGGA AGGTGAAGTA TTACTTTCAG CCAGACATGG GCAGCAGGAT CAACGCAGGT 
ACCCCCCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGACGAGGT AGCTCAGGCC 
GCCGAGGAGC TGAGACACAA AGGTGTGGAC ATCTACTCGG TGGGCATCGG GGATGTGGAT 
GACCAGGAAT TGGTCCAGAT CACGGGGACC GCGGAGAAAA AACTGACCGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGGAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGCT TTGTGGATGT CGTGGTTGGA TTTGACATCT CAAGCCTGCA GAGAGGGCAG 
ACTCTGCTCG AAGGTCAGCC TTGGATGGGA TCCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAAGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ACGCTATGGA AAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTAAGCAGCC TGCAGGGTGT GACCGTGAAT GGCCCGTCTC GCCTCAACGC CAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAATAAG TCAGCTGCTC GTGGGAAGGT GGTCCTTCTA 
TTTTCAGATG GACTGGATGA TGGCATTGAG AAACTTGAAC AGAAGTCTGA TGAACTCAGG 
AAGGAAGGCC TGAATGCCCT CATAACCATT GCTGTGGATG GAGCTGCCGA TTCCAGTGAC 
CTGGCGGACC TCCTCTACAT TGAATTTGGG AAAGGATTTG AATACAGGAC ACAGTTCACA 
ATTGGAATGA GGAACCTTGG GAGTCAGCTG TCAAGGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTCTGCTCTG CAAGTGCACA GGAGGGGACG GCGCCATGGG GGATCCAGGC 
TCAGCAGGAA AAAAGGGACC CCCAGGATTC AAAGGCAGTG ATGGCTACCT CGGAGAGGAG 
GGCATTGCTG GAGAAAGAGG AGCCTCTGGG CCAATGGGAG AGCAAGGCAC GAAGGGATGC 
TTCGGTGCCA AAGGGCCTAA GGGAACCAGA GGACTCAGTG GAGAAGAGGG TGAAGTCGGG 
GAAGATGGGC TTGATGGATT AGATGGAGAA CAGGGTGACC ATGGGATTCC TGGAAGAAGA 
GGAGAAAAGG GTGACGAGGG ATCTCAGGGA AACCCAGGCA GGAGAGGGGC TGCCGGTGAC 
CGTGGAGCAA AGGGACTGCG AGGAGATCCG GGAACTCCTG GACGTGACAG TAGCATACAA 
GGACCCAAGG GCTTGAAAGG AGACCTTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGAACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACACATAGGC 
CCACAGGGAA ATCCAGGAAC CCCGGGCCCA GATGGACTTG CAGGTTCGCC AGGACTTAGA 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAAG TCTGGGAATG 
AAAGGTCCTC AGGGGCCTCC AGGACCCGGA GGACAAGCTG GGAGCCAAGG CCATTTGGGA 
AGCCAAGGAA ACAAAGGAGA ACCTGGAGAC CTGGGAGAAA AGGGAGCTGC TGGCTTTCCA 
GGTCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCGGGAT ATGGTAGCAT TGGCCGCAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC TGAAGGGTGA TATTGGGGAC 
CCTGGTGATC CAGGAGAAGC TGGTCCCAAG GGAGCCAGAG GCAAAACGGT ATCTGCTGGG 
ATTCCAGGAG AGCCGGGGTC CCCCGGGGAG CCAGGACCCC CTGGACGGAA GGGTGTGAAA 
GGGGCCCGAG GACTGGCTTC GTTTTCCACA TGTGACCTCA TTCAGTACGT GCGGGACCAC 
AGTCCTGGCA GACATGGAAA ACCTGAGTGC CCAGTGCATC CAACTGAGTT GGTGTTTGTC 
CTGGACCAGT CCCGGGATGT CACAGAGCAG GACTTTGAGC GAATGAAGGG GATGATGGTC 
TCCCTAGTTA GGGATGTCAA GATGCCTACA GGAAGGACCA GCTGCTCACG GCGATTAAAG 
CTCTTCCTTA TGAGAGATCC TCCGACAGCA GGGAGATTGG CAAAGCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a6 ( XM_006511732.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511795.1 Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant X1, mRNA.

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CloneID	OMu43164
Clone ID Related Accession (Same CDS sequence)	XM_006511732.4 , XM_006511732.3
Accession Version	XM_006511732.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-6(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511732.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGG TTTTGTGCCT GACAATGATT TGTTTCCACG TGTGTGTGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGACGT GGTGTTTCTG GTGGACAGCT CCGATCACCT AGGGCTTAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAC AAGATGATCA GCAGCCTCCC CATAGAGGCC 
AACAAGTACC GCGTGGCCCT GGCCCAGTAC AGCGATGCTC TCCACAATGA GTTCCAGCTG 
GGCACCTTCA AGAACAGGAA CCCCATGCTG AACCACCTGA AGAAGAACTT CGGGTTCATC 
GGTGGCTCCC TGAAGATAGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAATG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCA 
GAGTCTGAGG ATGATGTGGA AGAGGCTGCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAAACCTGA AGGCGATGGC CACCTCTCAG 
TTCCATTTCA ATCTCAGGAC TGCCAGAGAC CTCAGCGTGT TTGCCCCAAA CATGACAGAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GGAATGGCAG ATGACATTAT TGTAGAAGCC 
TGCCAAGGCC CTTCTGTGGC TGATGTGGTG TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAGGAGGACC TAGATCATCT TAAAGCATTC CTGGGCGAAA GCATCTCTGC CCTGGACATA 
AAGGAAAATT GCATGAGGGT TGGCCTGGTG ACCTATAGCA ATGAGACAAG GGTGATCAGC 
TCTCTGAGCA CGGGTAACAA CAAGACAGAA GTCTTGCAGC GCATACAGGA TCTGTCCCCT 
CAAGTAGGGC AGGCCTACAC TGGAGCTGCC CTCAGAAAGA CTAGGAAGGA AATCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTCCCTCAGA TCGCTGTGCT GGTGACCCAC 
AGAGCATCAG AAGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
ATCTTTACCA TGGGCATAGA GGGGGCTAAC CCAGACGAGC TGGAGAAGAT CGCATCCCAC 
CCTGCGGAGC AGTTCACCTC CAAACTGGGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAACTGCG GAACCAAATC ACACACACGG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCTGC CTGTGTGGAC ACAGAGGAAG CCGATATCTA TCTACTCATT 
GATGGTTCAG GGAGCACCCA GCCCACAGAC TTCCATGAAA TGAAGACCTT CCTGTCAGAG 
GTGGTAGGCA TGTTCAACAT TGCTCCCCAC AAGGTGCGAG TAGGGGCCGT GCAGTACGCC 
GACACCTGGG ACTTGGAATT TGAGATCTCT AAGTATAGTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGAGA ATATCAGGCA GATGGGTGGG AATACCAACA CAGGGGCGGC TTTGAACTTC 
ACACTGAAGC TGTTGCAAAG AGCAAAGAAG GAACGAGGAA GCAAAGTGCC GTGTCACCTG 
GTGGTTCTGA CCAATGGCAT GTCTCGGGAC AGCGTCCTGG GGCCTGCCCA TAAGCTGAGA 
GAGGAAAACA TCAGAGTGCA TGCGATCGGT GTCAAGGAAG CCAACCAAAC GCAGCTTCGG 
GAGATAGCGG GAGAGGAAAA GCGAGTTTAC TACGTCCATG AGTTCGATGC CTTGAGGAAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA CATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATCGGAC CTGAAAACTT CAGCAAGATG 
AAGATGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGCGTGGTCC AGTTCAGCCA CGAAAACAAG GAGGAGTTTC AGCTCAACAC GTTCATGTCT 
CAAAGTGACA TCGCCAACGC CATTGACCGA ATGACTCACA TTGGAGAAAC AACCTTGACG 
GGCAGTGCCC TGACCTTTGT GTCTCAGTAC TTCAGTCCCG ATAAGGGGGC CAGGCCCAAT 
GTCAGGAAGT TCCTCATTCT TATCACGGAT GGTGAGGCTC AGGACATAGT AAGGGACCCA 
GCGATCGCCC TTCGAAAAGA AGGTGTGATT ATCTATTCTG TGGGAGTATT CGGCTCCAAT 
GTCACCCAGC TTGAGGAGAT CAGTGGAAAG CCAGAGATGG TTTTCTATGT TGAGAATTTT 
GACATTCTGC AGCATATCGA AGATGACCTC GTTCTGGGGA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTGGTG TTTGTCATCG ATAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCCCTG AAGTATGCTG ATGACCCCGA AGTGCTGTTT 
TACCTGGATG AACTAGGCAC GAAGCTGGAG GTAGTTTCAG TGCTCCAGAA TGACCATCCC 
ATGGGTGGAA ATACTTACAC CGCTGAGGCC CTCGCCTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACCGACGGG 
GAATCTCATG ACGCAGAGAA GCTCAACACC ACCGCCAAGG CCCTGAGAGA CAAAGGCATT 
CTCGTCCTGG CTGTGGGGAT TGCCGGTGCC AACAGCTGGG AGCTCTTGGC CATGGCAGGG 
TCAAGCGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGAAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTTGATTGTG AAATTGAAAA GGTTGACCTT 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGGG 
TTTTTGGTGT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGCGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACGTTCAC CGGGGAGAGG 
GAGATATCCA CCCAGATTGA GGGCATCCAG CAGATCTTTG GATACACCCA CATCGGAGAT 
GCTCTCAGGA AGGTGAAGTA TTACTTTCAG CCAGACATGG GCAGCAGGAT CAACGCAGGT 
ACCCCCCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGACGAGGT AGCTCAGGCC 
GCCGAGGAGC TGAGACACAA AGGTGTGGAC ATCTACTCGG TGGGCATCGG GGATGTGGAT 
GACCAGGAAT TGGTCCAGAT CACGGGGACC GCGGAGAAAA AACTGACCGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGGAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGCT TTGTGGATGT CGTGGTTGGA TTTGACATCT CAAGCCTGCA GAGAGGGCAG 
ACTCTGCTCG AAGGTCAGCC TTGGATGGGA TCCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAAGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ACGCTATGGA AAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTAAGCAGCC TGCAGGGTGT GACCGTGAAT GGCCCGTCTC GCCTCAACGC CAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAATAAG TCAGCTGCTC GTGGGAAGGT GGTCCTTCTA 
TTTTCAGATG GACTGGATGA TGGCATTGAG AAACTTGAAC AGAAGTCTGA TGAACTCAGG 
AAGGAAGGCC TGAATGCCCT CATAACCATT GCTGTGGATG GAGCTGCCGA TTCCAGTGAC 
CTGGCGGACC TCCTCTACAT TGAATTTGGG AAAGGATTTG AATACAGGAC ACAGTTCACA 
ATTGGAATGA GGAACCTTGG GAGTCAGCTG TCAAGGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTCTGCTCTG CAAGTGCACA GGAGGGGACG GCGCCATGGG GGATCCAGGC 
TCAGCAGGAA AAAAGGGACC CCCAGGATTC AAAGGCAGTG ATGGCTACCT CGGAGAGGAG 
GGCATTGCTG GAGAAAGAGG AGCCTCTGGG CCAATGGGAG AGCAAGGCAC GAAGGGATGC 
TTCGGTGCCA AAGGGCCTAA GGGAACCAGA GGACTCAGTG GAGAAGAGGG TGAAGTCGGG 
GAAGATGGGC TTGATGGATT AGATGGAGAA CAGGGTGACC ATGGGATTCC TGGAAGAAGA 
GGAGAAAAGG GTGACGAGGG ATCTCAGGGA AACCCAGGCA GGAGAGGGGC TGCCGGTGAC 
CGTGGAGCAA AGGGACTGCG AGGAGATCCG GGAACTCCTG GACGTGACAG TAGCATACAA 
GGACCCAAGG GCTTGAAAGG AGACCTTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGAACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACACATAGGC 
CCACAGGGAA ATCCAGGAAC CCCGGGCCCA GATGGACTTG CAGGTTCGCC AGGACTTAGA 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAAG TCTGGGAATG 
AAAGGTCCTC AGGGGCCTCC AGGACCCGGA GGACAAGCTG GGAGCCAAGG CCATTTGGGA 
AGCCAAGGAA ACAAAGGAGA ACCTGGAGAC CTGGGAGAAA AGGGAGCTGC TGGCTTTCCA 
GGTCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCGGGAT ATGGTAGCAT TGGCCGCAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC TGAAGGGTGA TATTGGGGAC 
CCTGGTGATC CAGGAGAAGC TGGTCCCAAG GGAGCCAGAG GCAAAACGGT ATCTGCTGGG 
ATTCCAGGAG AGCCGGGGTC CCCCGGGGAG CCAGGACCCC CTGGACGGAA GGGTGTGAAA 
GGGGCCCGAG GACTGGCTTC GTTTTCCACA TGTGACCTCA TTCAGTACGT GCGGGACCAC 
AGTCCTGGCA GACATGGAAA ACCTGAGTGC CCAGTGCATC CAACTGAGTT GGTGTTTGTC 
CTGGACCAGT CCCGGGATGT CACAGAGCAG GACTTTGAGC GAATGAAGGG GATGATGGTC 
TCCCTAGTTA GGGATGTCAA GATGCCTACA GGAAGGACCA GCTGCTCACG GCGATTAAAG 
CTCTTCCTTA TGAGAGATCC TCCGACAGCA GGGAGATTGG CAAAGCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511795.1
CDS	381..5831
Translation	MLLVLCLTMICFHVCVNQDSGPEYADVVFLVDSSDHLGLKSFPLVKTFIHKMISSLPIEANKYRVALAQYSDALHNEFQLGTFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEAAKALREDGVKIISVGVQKASEENLKAMATSQFHFNLRTARDLSVFAPNMTEIIKDVTQYREGMADDIIVEACQGPSVADVVFLLDMAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNVTKAAVNLRREGVTIFTMGIEGANPDELEKIASHPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSRDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEICAEEACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITDGEAQDIVRDPAIALRKEGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQDEVAQAAEELRHKGVDIYSVGIGDVDDQELVQITGTAEKKLTVHNFDELKKVKKRIVRNICTSGGESNCFVDVVVGFDISSLQRGQTLLEGQPWMGSYLQDLLRAISSLNGVSCEVGTETQVSIAFQVTNAMERYPSKFEIYSENILSSLQGVTVNGPSRLNANLLSSLWDTFQNKSAARGKVVLLFSDGLDDGIEKLEQKSDELRKEGLNALITIAVDGAADSSDLADLLYIEFGKGFEYRTQFTIGMRNLGSQLSRQLINVAERTCCCLLCKCTGGDGAMGDPGSAGKKGPPGFKGSDGYLGEEGIAGERGASGPMGEQGTKGCFGAKGPKGTRGLSGEEGEVGEDGLDGLDGEQGDHGIPGRRGEKGDEGSQGNPGRRGAAGDRGAKGLRGDPGTPGRDSSIQGPKGLKGDLGRQGRRGWPGSPGTPGSRRKMVVHGRRGHIGPQGNPGTPGPDGLAGSPGLRGPQGPRGEVGEKGEKGSLGMKGPQGPPGPGGQAGSQGHLGSQGNKGEPGDLGEKGAAGFPGPRGLQGDDGSPGYGSIGRKGTKGQEGFPGESGLKGDIGDPGDPGEAGPKGARGKTVSAGIPGEPGSPGEPGPPGRKGVKGARGLASFSTCDLIQYVRDHSPGRHGKPECPVHPTELVFVLDQSRDVTEQDFERMKGMMVSLVRDVKMPTGRTSCSRRLKLFLMRDPPTAGRLAKP

Target ORF information:

RefSeq Version	XM_006511732.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511732.3

ORF Insert Sequence:

ATGCTGCTGG TTTTGTGCCT GACAATGATT TGTTTCCACG TGTGTGTGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGACGT GGTGTTTCTG GTGGACAGCT CCGATCACCT AGGGCTTAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAC AAGATGATCA GCAGCCTCCC CATAGAGGCC 
AACAAGTACC GCGTGGCCCT GGCCCAGTAC AGCGATGCTC TCCACAATGA GTTCCAGCTG 
GGCACCTTCA AGAACAGGAA CCCCATGCTG AACCACCTGA AGAAGAACTT CGGGTTCATC 
GGTGGCTCCC TGAAGATAGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAATG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCA 
GAGTCTGAGG ATGATGTGGA AGAGGCTGCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAAACCTGA AGGCGATGGC CACCTCTCAG 
TTCCATTTCA ATCTCAGGAC TGCCAGAGAC CTCAGCGTGT TTGCCCCAAA CATGACAGAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GGAATGGCAG ATGACATTAT TGTAGAAGCC 
TGCCAAGGCC CTTCTGTGGC TGATGTGGTG TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAGGAGGACC TAGATCATCT TAAAGCATTC CTGGGCGAAA GCATCTCTGC CCTGGACATA 
AAGGAAAATT GCATGAGGGT TGGCCTGGTG ACCTATAGCA ATGAGACAAG GGTGATCAGC 
TCTCTGAGCA CGGGTAACAA CAAGACAGAA GTCTTGCAGC GCATACAGGA TCTGTCCCCT 
CAAGTAGGGC AGGCCTACAC TGGAGCTGCC CTCAGAAAGA CTAGGAAGGA AATCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTCCCTCAGA TCGCTGTGCT GGTGACCCAC 
AGAGCATCAG AAGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
ATCTTTACCA TGGGCATAGA GGGGGCTAAC CCAGACGAGC TGGAGAAGAT CGCATCCCAC 
CCTGCGGAGC AGTTCACCTC CAAACTGGGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAACTGCG GAACCAAATC ACACACACGG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCTGC CTGTGTGGAC ACAGAGGAAG CCGATATCTA TCTACTCATT 
GATGGTTCAG GGAGCACCCA GCCCACAGAC TTCCATGAAA TGAAGACCTT CCTGTCAGAG 
GTGGTAGGCA TGTTCAACAT TGCTCCCCAC AAGGTGCGAG TAGGGGCCGT GCAGTACGCC 
GACACCTGGG ACTTGGAATT TGAGATCTCT AAGTATAGTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGAGA ATATCAGGCA GATGGGTGGG AATACCAACA CAGGGGCGGC TTTGAACTTC 
ACACTGAAGC TGTTGCAAAG AGCAAAGAAG GAACGAGGAA GCAAAGTGCC GTGTCACCTG 
GTGGTTCTGA CCAATGGCAT GTCTCGGGAC AGCGTCCTGG GGCCTGCCCA TAAGCTGAGA 
GAGGAAAACA TCAGAGTGCA TGCGATCGGT GTCAAGGAAG CCAACCAAAC GCAGCTTCGG 
GAGATAGCGG GAGAGGAAAA GCGAGTTTAC TACGTCCATG AGTTCGATGC CTTGAGGAAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA CATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATCGGAC CTGAAAACTT CAGCAAGATG 
AAGATGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGCGTGGTCC AGTTCAGCCA CGAAAACAAG GAGGAGTTTC AGCTCAACAC GTTCATGTCT 
CAAAGTGACA TCGCCAACGC CATTGACCGA ATGACTCACA TTGGAGAAAC AACCTTGACG 
GGCAGTGCCC TGACCTTTGT GTCTCAGTAC TTCAGTCCCG ATAAGGGGGC CAGGCCCAAT 
GTCAGGAAGT TCCTCATTCT TATCACGGAT GGTGAGGCTC AGGACATAGT AAGGGACCCA 
GCGATCGCCC TTCGAAAAGA AGGTGTGATT ATCTATTCTG TGGGAGTATT CGGCTCCAAT 
GTCACCCAGC TTGAGGAGAT CAGTGGAAAG CCAGAGATGG TTTTCTATGT TGAGAATTTT 
GACATTCTGC AGCATATCGA AGATGACCTC GTTCTGGGGA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTGGTG TTTGTCATCG ATAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCCCTG AAGTATGCTG ATGACCCCGA AGTGCTGTTT 
TACCTGGATG AACTAGGCAC GAAGCTGGAG GTAGTTTCAG TGCTCCAGAA TGACCATCCC 
ATGGGTGGAA ATACTTACAC CGCTGAGGCC CTCGCCTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACCGACGGG 
GAATCTCATG ACGCAGAGAA GCTCAACACC ACCGCCAAGG CCCTGAGAGA CAAAGGCATT 
CTCGTCCTGG CTGTGGGGAT TGCCGGTGCC AACAGCTGGG AGCTCTTGGC CATGGCAGGG 
TCAAGCGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGAAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTTGATTGTG AAATTGAAAA GGTTGACCTT 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGGG 
TTTTTGGTGT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGCGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACGTTCAC CGGGGAGAGG 
GAGATATCCA CCCAGATTGA GGGCATCCAG CAGATCTTTG GATACACCCA CATCGGAGAT 
GCTCTCAGGA AGGTGAAGTA TTACTTTCAG CCAGACATGG GCAGCAGGAT CAACGCAGGT 
ACCCCCCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGACGAGGT AGCTCAGGCC 
GCCGAGGAGC TGAGACACAA AGGTGTGGAC ATCTACTCGG TGGGCATCGG GGATGTGGAT 
GACCAGGAAT TGGTCCAGAT CACGGGGACC GCGGAGAAAA AACTGACCGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGGAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGCT TTGTGGATGT CGTGGTTGGA TTTGACATCT CAAGCCTGCA GAGAGGGCAG 
ACTCTGCTCG AAGGTCAGCC TTGGATGGGA TCCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAAGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ACGCTATGGA AAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTAAGCAGCC TGCAGGGTGT GACCGTGAAT GGCCCGTCTC GCCTCAACGC CAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAATAAG TCAGCTGCTC GTGGGAAGGT GGTCCTTCTA 
TTTTCAGATG GACTGGATGA TGGCATTGAG AAACTTGAAC AGAAGTCTGA TGAACTCAGG 
AAGGAAGGCC TGAATGCCCT CATAACCATT GCTGTGGATG GAGCTGCCGA TTCCAGTGAC 
CTGGCGGACC TCCTCTACAT TGAATTTGGG AAAGGATTTG AATACAGGAC ACAGTTCACA 
ATTGGAATGA GGAACCTTGG GAGTCAGCTG TCAAGGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTCTGCTCTG CAAGTGCACA GGAGGGGACG GCGCCATGGG GGATCCAGGC 
TCAGCAGGAA AAAAGGGACC CCCAGGATTC AAAGGCAGTG ATGGCTACCT CGGAGAGGAG 
GGCATTGCTG GAGAAAGAGG AGCCTCTGGG CCAATGGGAG AGCAAGGCAC GAAGGGATGC 
TTCGGTGCCA AAGGGCCTAA GGGAACCAGA GGACTCAGTG GAGAAGAGGG TGAAGTCGGG 
GAAGATGGGC TTGATGGATT AGATGGAGAA CAGGGTGACC ATGGGATTCC TGGAAGAAGA 
GGAGAAAAGG GTGACGAGGG ATCTCAGGGA AACCCAGGCA GGAGAGGGGC TGCCGGTGAC 
CGTGGAGCAA AGGGACTGCG AGGAGATCCG GGAACTCCTG GACGTGACAG TAGCATACAA 
GGACCCAAGG GCTTGAAAGG AGACCTTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGAACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACACATAGGC 
CCACAGGGAA ATCCAGGAAC CCCGGGCCCA GATGGACTTG CAGGTTCGCC AGGACTTAGA 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAAG TCTGGGAATG 
AAAGGTCCTC AGGGGCCTCC AGGACCCGGA GGACAAGCTG GGAGCCAAGG CCATTTGGGA 
AGCCAAGGAA ACAAAGGAGA ACCTGGAGAC CTGGGAGAAA AGGGAGCTGC TGGCTTTCCA 
GGTCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCGGGAT ATGGTAGCAT TGGCCGCAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC TGAAGGGTGA TATTGGGGAC 
CCTGGTGATC CAGGAGAAGC TGGTCCCAAG GGAGCCAGAG GCAAAACGGT ATCTGCTGGG 
ATTCCAGGAG AGCCGGGGTC CCCCGGGGAG CCAGGACCCC CTGGACGGAA GGGTGTGAAA 
GGGGCCCGAG GACTGGCTTC GTTTTCCACA TGTGACCTCA TTCAGTACGT GCGGGACCAC 
AGTCCTGGCA GACATGGAAA ACCTGAGTGC CCAGTGCATC CAACTGAGTT GGTGTTTGTC 
CTGGACCAGT CCCGGGATGT CACAGAGCAG GACTTTGAGC GAATGAAGGG GATGATGGTC 
TCCCTAGTTA GGGATGTCAA GATGCCTACA GGAAGGACCA GCTGCTCACG GCGATTAAAG 
CTCTTCCTTA TGAGAGATCC TCCGACAGCA GGGAGATTGG CAAAGCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a6 ( XM_006511733.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511796.1 Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant X2, mRNA.

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CloneID	OMu43165
Clone ID Related Accession (Same CDS sequence)	XM_006511733.2 , XM_006511733.3
Accession Version	XM_006511733.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-6(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006511733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGG TTTTGTGCCT GACAATGATT TGTTTCCACG TGTGTGTGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGACGT GGTGTTTCTG GTGGACAGCT CCGATCACCT AGGGCTTAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAC AAGATGATCA GCAGCCTCCC CATAGAGGCC 
AACAAGTACC GCGTGGCCCT GGCCCAGTAC AGCGATGCTC TCCACAATGA GTTCCAGCTG 
GGCACCTTCA AGAACAGGAA CCCCATGCTG AACCACCTGA AGAAGAACTT CGGGTTCATC 
GGTGGCTCCC TGAAGATAGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAATG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCA 
GAGTCTGAGG ATGATGTGGA AGAGGCTGCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAAACCTGA AGGCGATGGC CACCTCTCAG 
TTCCATTTCA ATCTCAGGAC TGCCAGAGAC CTCAGCGTGT TTGCCCCAAA CATGACAGAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GGAATGGCAG ATGACATTAT TGTAGAAGCC 
TGCCAAGGCC CTTCTGTGGC TGATGTGGTG TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAGGAGGACC TAGATCATCT TAAAGCATTC CTGGGCGAAA GCATCTCTGC CCTGGACATA 
AAGGAAAATT GCATGAGGGT TGGCCTGGTG ACCTATAGCA ATGAGACAAG GGTGATCAGC 
TCTCTGAGCA CGGGTAACAA CAAGACAGAA GTCTTGCAGC GCATACAGGA TCTGTCCCCT 
CAAGTAGGGC AGGCCTACAC TGGAGCTGCC CTCAGAAAGA CTAGGAAGGA AATCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTCCCTCAGA TCGCTGTGCT GGTGACCCAC 
AGAGCATCAG AAGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
ATCTTTACCA TGGGCATAGA GGGGGCTAAC CCAGACGAGC TGGAGAAGAT CGCATCCCAC 
CCTGCGGAGC AGTTCACCTC CAAACTGGGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAACTGCG GAACCAAATC ACACACACGG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCTGC CTGTGTGGAC ACAGAGGAAG CCGATATCTA TCTACTCATT 
GATGGTTCAG GGAGCACCCA GCCCACAGAC TTCCATGAAA TGAAGACCTT CCTGTCAGAG 
GTGGTAGGCA TGTTCAACAT TGCTCCCCAC AAGGTGCGAG TAGGGGCCGT GCAGTACGCC 
GACACCTGGG ACTTGGAATT TGAGATCTCT AAGTATAGTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGAGA ATATCAGGCA GATGGGTGGG AATACCAACA CAGGGGCGGC TTTGAACTTC 
ACACTGAAGC TGTTGCAAAG AGCAAAGAAG GAACGAGGAA GCAAAGTGCC GTGTCACCTG 
GTGGTTCTGA CCAATGGCAT GTCTCGGGAC AGCGTCCTGG GGCCTGCCCA TAAGCTGAGA 
GAGGAAAACA TCAGAGTGCA TGCGATCGGT GTCAAGGAAG CCAACCAAAC GCAGCTTCGG 
GAGATAGCGG GAGAGGAAAA GCGAGTTTAC TACGTCCATG AGTTCGATGC CTTGAGGAAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA CATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATCGGAC CTGAAAACTT CAGCAAGATG 
AAGATGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGCGTGGTCC AGTTCAGCCA CGAAAACAAG GAGGAGTTTC AGCTCAACAC GTTCATGTCT 
CAAAGTGACA TCGCCAACGC CATTGACCGA ATGACTCACA TTGGAGAAAC AACCTTGACG 
GGCAGTGCCC TGACCTTTGT GTCTCAGTAC TTCAGTCCCG ATAAGGGGGC CAGGCCCAAT 
GTCAGGAAGT TCCTCATTCT TATCACGGAT GGTGAGGCTC AGGACATAGT AAGGGACCCA 
GCGATCGCCC TTCGAAAAGA AGGTGTGATT ATCTATTCTG TGGGAGTATT CGGCTCCAAT 
GTCACCCAGC TTGAGGAGAT CAGTGGAAAG CCAGAGATGG TTTTCTATGT TGAGAATTTT 
GACATTCTGC AGCATATCGA AGATGACCTC GTTCTGGGGA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTGGTG TTTGTCATCG ATAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCCCTG AAGTATGCTG ATGACCCCGA AGTGCTGTTT 
TACCTGGATG AACTAGGCAC GAAGCTGGAG GTAGTTTCAG TGCTCCAGAA TGACCATCCC 
ATGGGTGGAA ATACTTACAC CGCTGAGGCC CTCGCCTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACCGACGGG 
GAATCTCATG ACGCAGAGAA GCTCAACACC ACCGCCAAGG CCCTGAGAGA CAAAGGCATT 
CTCGTCCTGG CTGTGGGGAT TGCCGGTGCC AACAGCTGGG AGCTCTTGGC CATGGCAGGG 
TCAAGCGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGAAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTTGATTGTG AAATTGAAAA GGTTGACCTT 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGGG 
TTTTTGGTGT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGCGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACGTTCAC CGGGGAGAGG 
GAGATATCCA CCCAGATTGA GGGCATCCAG CAGATCTTTG GATACACCCA CATCGGAGAT 
GCTCTCAGGA AGGTGAAGTA TTACTTTCAG CCAGACATGG GCAGCAGGAT CAACGCAGGT 
ACCCCCCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGACGAGGT AGCTCAGGCC 
GCCGAGGAGC TGAGACACAA AGGTGTGGAC ATCTACTCGG TGGGCATCGG GGATGTGGAT 
GACCAGGAAT TGGTCCAGAT CACGGGGACC GCGGAGAAAA AACTGACCGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGGAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGCT TTGTGGATGT CGTGGTTGGA TTTGACATCT CAAGCCTGCA GAGAGGGCAG 
ACTCTGCTCG AAGGTCAGCC TTGGATGGGA TCCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAAGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ACGCTATGGA AAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTAAGCAGCC TGCAGGGTGT GACCGTGAAT GGCCCGTCTC GCCTCAACGC CAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAATAAG TCAGCTGCTC GTGGGAAGGT GGTCCTTCTA 
TTTTCAGATG GACTGGATGA TGGCATTGAG AAACTTGAAC AGAAGTCTGA TGAACTCAGG 
AAGGAAGCAT TGCATCATGG GATTAGAGGA CATAGACAAT GGAGAGCAGC TGTGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511796.1
CDS	381..4400
Translation	MLLVLCLTMICFHVCVNQDSGPEYADVVFLVDSSDHLGLKSFPLVKTFIHKMISSLPIEANKYRVALAQYSDALHNEFQLGTFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEAAKALREDGVKIISVGVQKASEENLKAMATSQFHFNLRTARDLSVFAPNMTEIIKDVTQYREGMADDIIVEACQGPSVADVVFLLDMAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNVTKAAVNLRREGVTIFTMGIEGANPDELEKIASHPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSRDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEICAEEACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITDGEAQDIVRDPAIALRKEGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQDEVAQAAEELRHKGVDIYSVGIGDVDDQELVQITGTAEKKLTVHNFDELKKVKKRIVRNICTSGGESNCFVDVVVGFDISSLQRGQTLLEGQPWMGSYLQDLLRAISSLNGVSCEVGTETQVSIAFQVTNAMERYPSKFEIYSENILSSLQGVTVNGPSRLNANLLSSLWDTFQNKSAARGKVVLLFSDGLDDGIEKLEQKSDELRKEALHHGIRGHRQWRAAVD

Target ORF information:

RefSeq Version	XM_006511733.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511733.2

ORF Insert Sequence:

ATGCTGCTGG TTTTGTGCCT GACAATGATT TGTTTCCACG TGTGTGTGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGACGT GGTGTTTCTG GTGGACAGCT CCGATCACCT AGGGCTTAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAC AAGATGATCA GCAGCCTCCC CATAGAGGCC 
AACAAGTACC GCGTGGCCCT GGCCCAGTAC AGCGATGCTC TCCACAATGA GTTCCAGCTG 
GGCACCTTCA AGAACAGGAA CCCCATGCTG AACCACCTGA AGAAGAACTT CGGGTTCATC 
GGTGGCTCCC TGAAGATAGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAATG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCA 
GAGTCTGAGG ATGATGTGGA AGAGGCTGCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAAACCTGA AGGCGATGGC CACCTCTCAG 
TTCCATTTCA ATCTCAGGAC TGCCAGAGAC CTCAGCGTGT TTGCCCCAAA CATGACAGAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GGAATGGCAG ATGACATTAT TGTAGAAGCC 
TGCCAAGGCC CTTCTGTGGC TGATGTGGTG TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAGGAGGACC TAGATCATCT TAAAGCATTC CTGGGCGAAA GCATCTCTGC CCTGGACATA 
AAGGAAAATT GCATGAGGGT TGGCCTGGTG ACCTATAGCA ATGAGACAAG GGTGATCAGC 
TCTCTGAGCA CGGGTAACAA CAAGACAGAA GTCTTGCAGC GCATACAGGA TCTGTCCCCT 
CAAGTAGGGC AGGCCTACAC TGGAGCTGCC CTCAGAAAGA CTAGGAAGGA AATCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTCCCTCAGA TCGCTGTGCT GGTGACCCAC 
AGAGCATCAG AAGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
ATCTTTACCA TGGGCATAGA GGGGGCTAAC CCAGACGAGC TGGAGAAGAT CGCATCCCAC 
CCTGCGGAGC AGTTCACCTC CAAACTGGGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAACTGCG GAACCAAATC ACACACACGG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCTGC CTGTGTGGAC ACAGAGGAAG CCGATATCTA TCTACTCATT 
GATGGTTCAG GGAGCACCCA GCCCACAGAC TTCCATGAAA TGAAGACCTT CCTGTCAGAG 
GTGGTAGGCA TGTTCAACAT TGCTCCCCAC AAGGTGCGAG TAGGGGCCGT GCAGTACGCC 
GACACCTGGG ACTTGGAATT TGAGATCTCT AAGTATAGTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGAGA ATATCAGGCA GATGGGTGGG AATACCAACA CAGGGGCGGC TTTGAACTTC 
ACACTGAAGC TGTTGCAAAG AGCAAAGAAG GAACGAGGAA GCAAAGTGCC GTGTCACCTG 
GTGGTTCTGA CCAATGGCAT GTCTCGGGAC AGCGTCCTGG GGCCTGCCCA TAAGCTGAGA 
GAGGAAAACA TCAGAGTGCA TGCGATCGGT GTCAAGGAAG CCAACCAAAC GCAGCTTCGG 
GAGATAGCGG GAGAGGAAAA GCGAGTTTAC TACGTCCATG AGTTCGATGC CTTGAGGAAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA CATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATCGGAC CTGAAAACTT CAGCAAGATG 
AAGATGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGCGTGGTCC AGTTCAGCCA CGAAAACAAG GAGGAGTTTC AGCTCAACAC GTTCATGTCT 
CAAAGTGACA TCGCCAACGC CATTGACCGA ATGACTCACA TTGGAGAAAC AACCTTGACG 
GGCAGTGCCC TGACCTTTGT GTCTCAGTAC TTCAGTCCCG ATAAGGGGGC CAGGCCCAAT 
GTCAGGAAGT TCCTCATTCT TATCACGGAT GGTGAGGCTC AGGACATAGT AAGGGACCCA 
GCGATCGCCC TTCGAAAAGA AGGTGTGATT ATCTATTCTG TGGGAGTATT CGGCTCCAAT 
GTCACCCAGC TTGAGGAGAT CAGTGGAAAG CCAGAGATGG TTTTCTATGT TGAGAATTTT 
GACATTCTGC AGCATATCGA AGATGACCTC GTTCTGGGGA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTGGTG TTTGTCATCG ATAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCCCTG AAGTATGCTG ATGACCCCGA AGTGCTGTTT 
TACCTGGATG AACTAGGCAC GAAGCTGGAG GTAGTTTCAG TGCTCCAGAA TGACCATCCC 
ATGGGTGGAA ATACTTACAC CGCTGAGGCC CTCGCCTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACCGACGGG 
GAATCTCATG ACGCAGAGAA GCTCAACACC ACCGCCAAGG CCCTGAGAGA CAAAGGCATT 
CTCGTCCTGG CTGTGGGGAT TGCCGGTGCC AACAGCTGGG AGCTCTTGGC CATGGCAGGG 
TCAAGCGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGAAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTTGATTGTG AAATTGAAAA GGTTGACCTT 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGGG 
TTTTTGGTGT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGCGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACGTTCAC CGGGGAGAGG 
GAGATATCCA CCCAGATTGA GGGCATCCAG CAGATCTTTG GATACACCCA CATCGGAGAT 
GCTCTCAGGA AGGTGAAGTA TTACTTTCAG CCAGACATGG GCAGCAGGAT CAACGCAGGT 
ACCCCCCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGACGAGGT AGCTCAGGCC 
GCCGAGGAGC TGAGACACAA AGGTGTGGAC ATCTACTCGG TGGGCATCGG GGATGTGGAT 
GACCAGGAAT TGGTCCAGAT CACGGGGACC GCGGAGAAAA AACTGACCGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGGAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGCT TTGTGGATGT CGTGGTTGGA TTTGACATCT CAAGCCTGCA GAGAGGGCAG 
ACTCTGCTCG AAGGTCAGCC TTGGATGGGA TCCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAAGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ACGCTATGGA AAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTAAGCAGCC TGCAGGGTGT GACCGTGAAT GGCCCGTCTC GCCTCAACGC CAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAATAAG TCAGCTGCTC GTGGGAAGGT GGTCCTTCTA 
TTTTCAGATG GACTGGATGA TGGCATTGAG AAACTTGAAC AGAAGTCTGA TGAACTCAGG 
AAGGAAGCAT TGCATCATGG GATTAGAGGA CATAGACAAT GGAGAGCAGC TGTGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a6 ( NM_001102607.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001096077.1 Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant 1, mRNA.

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CloneID	OMu21016
Clone ID Related Accession (Same CDS sequence)	NM_001102607.1
Accession Version	NM_001102607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	collagen alpha-6(VI) chain isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK087828.1, AM748261.1 and AM748262.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCTGCTGG TTTTGTGCCT GACAATGATT TGTTTCCACG TGTGTGTGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGACGT GGTGTTTCTG GTGGACAGCT CCGATCACCT AGGGCTTAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAC AAGATGATCA GCAGCCTCCC CATAGAGGCC 
AACAAGTACC GCGTGGCCCT GGCCCAGTAC AGCGATGCTC TCCACAATGA GTTCCAGCTG 
GGCACCTTCA AGAACAGGAA CCCCATGCTG AACCACCTGA AGAAGAACTT CGGGTTCATC 
GGTGGCTCCC TGAAGATAGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAATG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCA 
GAGTCTGAGG ATGATGTGGA AGAGGCTGCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAAACCTGA AGGCGATGGC CACCTCTCAG 
TTCCATTTCA ATCTCAGGAC TGCCAGAGAC CTCAGCGTGT TTGCCCCAAA CATGACAGAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GGAATGGCAG ATGACATTAT TGTAGAAGCC 
TGCCAAGGCC CTTCTGTGGC TGATGTGGTG TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAGGAGGACC TAGATCATCT TAAAGCATTC CTGGGCGAAA GCATCTCTGC CCTGGACATA 
AAGGAAAATT GCATGAGGGT TGGCCTGGTG ACCTATAGCA ATGAGACAAG GGTGATCAGC 
TCTCTGAGCA CGGGTAACAA CAAGACAGAA GTCTTGCAGC GCATACAGGA TCTGTCCCCT 
CAAGTAGGGC AGGCCTACAC TGGAGCTGCC CTCAGAAAGA CTAGGAAGGA AATCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTCCCTCAGA TCGCTGTGCT GGTGACCCAC 
AGAGCATCAG AAGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
ATCTTTACCA TGGGCATAGA GGGGGCTAAC CCAGACGAGC TGGAGAAGAT CGCATCCCAC 
CCTGCGGAGC AGTTCACCTC CAAACTGGGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAACTGCG GAACCAAATC ACACACACGG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCTGC CTGTGTGGAC ACAGAGGAAG CCGATATCTA TCTACTCATT 
GATGGTTCAG GGAGCACCCA GCCCACAGAC TTCCATGAAA TGAAGACCTT CCTGTCAGAG 
GTGGTAGGCA TGTTCAACAT TGCTCCCCAC AAGGTGCGAG TAGGGGCCGT GCAGTACGCC 
GACACCTGGG ACTTGGAATT TGAGATCTCT AAGTATAGTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGAGA ATATCAGGCA GATGGGTGGG AATACCAACA CAGGGGCGGC TTTGAACTTC 
ACACTGAAGC TGTTGCAAAG AGCAAAGAAG GAACGAGGAA GCAAAGTGCC GTGTCACCTG 
GTGGTTCTGA CCAATGGCAT GTCTCGGGAC AGCGTCCTGG GGCCTGCCCA TAAGCTGAGA 
GAGGAAAACA TCAGAGTGCA TGCGATCGGT GTCAAGGAAG CCAACCAAAC GCAGCTTCGG 
GAGATAGCGG GAGAGGAAAA GCGAGTTTAC TACGTCCATG AGTTCGATGC CTTGAGGAAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA CATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATCGGAC CTGAAAACTT CAGCAAGATG 
AAGATGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGCGTGGTCC AGTTCAGCCA CGAAAACAAG GAGGAGTTTC AGCTCAACAC GTTCATGTCT 
CAAAGTGACA TCGCCAACGC CATTGACCGA ATGACTCACA TTGGAGAAAC AACCTTGACG 
GGCAGTGCCC TGACCTTTGT GTCTCAGTAC TTCAGTCCCG ATAAGGGGGC CAGGCCCAAT 
GTCAGGAAGT TCCTCATTCT TATCACGGAT GGTGAGGCTC AGGACATAGT AAGGGACCCA 
GCGATCGCCC TTCGAAAAGA AGGTGTGATT ATCTATTCTG TGGGAGTATT CGGCTCCAAT 
GTCACCCAGC TTGAGGAGAT CAGTGGAAAG CCAGAGATGG TTTTCTATGT TGAGAATTTT 
GACATTCTGC AGCATATCGA AGATGACCTC GTTCTGGGGA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTGGTG TTTGTCATCG ATAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCCCTG AAGTATGCTG ATGACCCCGA AGTGCTGTTT 
TACCTGGATG AACTAGGCAC GAAGCTGGAG GTAGTTTCAG TGCTCCAGAA TGACCATCCC 
ATGGGTGGAA ATACTTACAC CGCTGAGGCC CTCGCCTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACCGACGGG 
GAATCTCATG ACGCAGAGAA GCTCAACACC ACCGCCAAGG CCCTGAGAGA CAAAGGCATT 
CTCGTCCTGG CTGTGGGGAT TGCCGGTGCC AACAGCTGGG AGCTCTTGGC CATGGCAGGG 
TCAAGCGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGAAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTTGATTGTG AAATTGAAAA GGTTGACCTT 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGGG 
TTTTTGGTGT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGCGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACGTTCAC CGGGGAGAGG 
GAGATATCCA CCCAGATTGA GGGCATCCAG CAGATCTTTG GATACACCCA CATCGGAGAT 
GCTCTCAGGA AGGTGAAGTA TTACTTTCAG CCAGACATGG GCAGCAGGAT CAACGCAGGT 
ACCCCCCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGACGAGGT AGCTCAGGCC 
GCCGAGGAGC TGAGACACAA AGGTGTGGAC ATCTACTCGG TGGGCATCGG GGATGTGGAT 
GACCAGGAAT TGGTCCAGAT CACGGGGACC GCGGAGAAAA AACTGACCGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGGAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGCT TTGTGGATGT CGTGGTTGGA TTTGACATCT CAAGCCTGCA GAGAGGGCAG 
ACTCTGCTCG AAGGTCAGCC TTGGATGGGA TCCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAAGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ACGCTATGGA AAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTAAGCAGCC TGCAGGGTGT GACCGTGAAT GGCCCGTCTC GCCTCAACGC CAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAATAAG TCAGCTGCTC GTGGGAAGGT GGTCCTTCTA 
TTTTCAGATG GACTGGATGA TGGCATTGAG AAACTTGAAC AGAAGTCTGA TGAACTCAGG 
AAGGAAGGCC TGAATGCCCT CATAACCATT GCTGTGGATG GAGCTGCCGA TTCCAGTGAC 
CTGGCGGACC TCCTCTACAT TGAATTTGGG AAAGGATTTG AATACAGGAC ACAGTTCACA 
ATTGGAATGA GGAACCTTGG GAGTCAGCTG TCAAGGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTCTGCTCTG CAAGTGCACA GGAGGGGACG GCGCCATGGG GGATCCAGGC 
TCAGCAGGAA AAAAGGGACC CCCAGGATTC AAAGGCAGTG ATGGCTACCT CGGAGAGGAG 
GGCATTGCTG GAGAAAGAGG AGCCTCTGGG CCAATGGGAG AGCAAGGCAC GAAGGGATGC 
TTCGGTGCCA AAGGGCCTAA GGGAACCAGA GGACTCAGTG GAGAAGAGGG TGAAGTCGGG 
GAAGATGGGC TTGATGGATT AGATGGAGAA CAGGGTGACC ATGGGATTCC TGGAAGAAGA 
GGAGAAAAGG GTGACGAGGG ATCTCAGGGA AACCCAGGCA GGAGAGGGGC TGCCGGTGAC 
CGTGGAGCAA AGGGACTGCG AGGAGATCCG GGAACTCCTG GACGTGACAG TAGCATACAA 
GGACCCAAGG GCTTGAAAGG AGACCTTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGAACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACACATAGGC 
CCACAGGGAA ATCCAGGAAC CCCGGGCCCA GATGGACTTG CAGGTTCGCC AGGACTTAGA 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAAG TCTGGGAATG 
AAAGGTCCTC AGGGGCCTCC AGGACCCGGA GGACAAGCTG GGAGCCAAGG CCATTTGGGA 
AGCCAAGGAA ACAAAGGAGA ACCTGGAGAC CTGGGAGAAA AGGGAGCTGC TGGCTTTCCA 
GGTCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCGGGAT ATGGTAGCAT TGGCCGCAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC TGAAGGGTGA TATTGGGGAC 
CCTGGTGATC CAGGAGAAGC TGGTCCCAAG GGAGCCAGAG GCAAAACGGT ATCTGCTGGG 
ATTCCAGGAG AGCCGGGGTC CCCCGGGGAG CCAGGACCCC CTGGACGGAA GGGTGTGAAA 
GGGGCCCGAG GACTGGCTTC GTTTTCCACA TGTGACCTCA TTCAGTACGT GCGGGACCAC 
AGTCCTGGCA GACATGGAAA ACCTGAGTGC CCAGTGCATC CAACTGAGTT GGTGTTTGTC 
CTGGACCAGT CCCGGGATGT CACAGAGCAG GACTTTGAGC GAATGAAGGG GATGATGGTC 
TCCCTAGTTA GGGATGTCAA GGTTAGGGAG GCCAACTGCC CCGTGGGTGC CCGCGTTGCC 
ATCCTAGCCT ACAACTCCCA CACCAGGCAC CTCATCCGCT TCTCAGATGC CTACAGGAAG 
GACCAGCTGC TCACGGCGAT TAAAGCTCTT CCTTATGAGA GATCCTCCGA CAGCAGGGAG 
ATTGGCAAAG CCATGAGGTT CATCTCCAGG AATGTCTTCA AGAGAACCCT TCCAGGGGCT 
CATGTGAGAA GAATCGCCAC GTTCTTTAGC AGTGGTCCAT CGGCCGACGC CCAGACCATT 
ACCACAGCCG CCATGGAGTT CAGTGCCCTG GACATTGTAC CTGTGGTGAT TGCGTTCAGC 
AACGTGCCCT CGGTCAAGCG CGCCTTTTCA ATTGATGACA CTGGCACATT CCAAGTCATT 
GTGGTTCCCT CCGGGTCTGA TGAAGGGCCA GCGTTAGAGA GACTCCAGCG GTGCACTTTC 
TGCTATGATC TATGCAAGCC GGATGCTTCG TGTGACCAAG CCAAACCACC CCCCATTCAG 
TCCTACCTGG ACACTGCATT CCTACTGGAT GGCTCTCGGC ACGTGGGAAG TGCAGAGTTT 
GAAGACATGA GAGACTTTCT AGAGGCACTG CTAGATCACT TCGAAATCAC TTCGGAGCCA 
GAGACATCTG TCACTGGAGA CAGGGTGGCC CTGCTGAGCC ATGCCCCCCT CGACTTCCTA 
CCCAACACCC AGAGGAGTCC GGTTAGAACT GAGTTCAACC TAACCAGCTA TAGCAGTAAG 
CGCCTCATGA AGAGACACGT GGATCAGGCA GTCCAGCAGC TGCATGGAGA TGCTTTTCTT 
GGCCATGCCC TCGGGTGGGC ACTGGACAAC GTCTTTTTAA ACACCCCCAA TTTGAGACGA 
AACAAAGTCA TATTTGTGAT ATCTGCTGGG GAAACCAGCC ACCTGGATGC GGAAACCTTA 
AAGAAAGAGT CCCTACGAGC CAAATGTCAT GGGTATGCCC TCTTTGTCTT TTCCTTGGGC 
CCTGACTGGG ATGACAAGGA ACTGGAAGAC CTGGCCAGCC ACCCCGTAGA CCAGCACTTG 
ATCCAGCTTG GCCGAATTCA TAAACCTGAC CACGGATACA GTGTGAAGTT TGTGAAGTCT 
TTTATAAACT CCATCAGGCA TGGGATAAAC AAGTATCCAC CGGTCAACCT CAAAGCCAAG 
TGCAACAGAC TCGGCTCTAG GGATCTGAAG CCGCCCCCAC GCCAGTTTCG AAGCTTTGTT 
CCAGGACCAC AGAAAGCCAA CCTCAAAGAC CACACAGCAG AGGCAGCAAA GCTCTTTCAA 
GATAAAAAAC GCCTTTCAAG TATGCTGAAA GGGGGCAGAG CTACTATTTC AAGTCTTTCC 
AGAAGCACAC GCTATGCCTT TAAACAGGGG AAAGAAGCAA TAAAAGCCAC TTCTAAACTC 
GGTAAACGAA GTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096077.1
CDS	221..7018
Translation	MLLVLCLTMICFHVCVNQDSGPEYADVVFLVDSSDHLGLKSFPLVKTFIHKMISSLPIEANKYRVALAQYSDALHNEFQLGTFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEAAKALREDGVKIISVGVQKASEENLKAMATSQFHFNLRTARDLSVFAPNMTEIIKDVTQYREGMADDIIVEACQGPSVADVVFLLDMAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNVTKAAVNLRREGVTIFTMGIEGANPDELEKIASHPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSRDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEICAEEACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITDGEAQDIVRDPAIALRKEGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQDEVAQAAEELRHKGVDIYSVGIGDVDDQELVQITGTAEKKLTVHNFDELKKVKKRIVRNICTSGGESNCFVDVVVGFDISSLQRGQTLLEGQPWMGSYLQDLLRAISSLNGVSCEVGTETQVSIAFQVTNAMERYPSKFEIYSENILSSLQGVTVNGPSRLNANLLSSLWDTFQNKSAARGKVVLLFSDGLDDGIEKLEQKSDELRKEGLNALITIAVDGAADSSDLADLLYIEFGKGFEYRTQFTIGMRNLGSQLSRQLINVAERTCCCLLCKCTGGDGAMGDPGSAGKKGPPGFKGSDGYLGEEGIAGERGASGPMGEQGTKGCFGAKGPKGTRGLSGEEGEVGEDGLDGLDGEQGDHGIPGRRGEKGDEGSQGNPGRRGAAGDRGAKGLRGDPGTPGRDSSIQGPKGLKGDLGRQGRRGWPGSPGTPGSRRKMVVHGRRGHIGPQGNPGTPGPDGLAGSPGLRGPQGPRGEVGEKGEKGSLGMKGPQGPPGPGGQAGSQGHLGSQGNKGEPGDLGEKGAAGFPGPRGLQGDDGSPGYGSIGRKGTKGQEGFPGESGLKGDIGDPGDPGEAGPKGARGKTVSAGIPGEPGSPGEPGPPGRKGVKGARGLASFSTCDLIQYVRDHSPGRHGKPECPVHPTELVFVLDQSRDVTEQDFERMKGMMVSLVRDVKVREANCPVGARVAILAYNSHTRHLIRFSDAYRKDQLLTAIKALPYERSSDSREIGKAMRFISRNVFKRTLPGAHVRRIATFFSSGPSADAQTITTAAMEFSALDIVPVVIAFSNVPSVKRAFSIDDTGTFQVIVVPSGSDEGPALERLQRCTFCYDLCKPDASCDQAKPPPIQSYLDTAFLLDGSRHVGSAEFEDMRDFLEALLDHFEITSEPETSVTGDRVALLSHAPLDFLPNTQRSPVRTEFNLTSYSSKRLMKRHVDQAVQQLHGDAFLGHALGWALDNVFLNTPNLRRNKVIFVISAGETSHLDAETLKKESLRAKCHGYALFVFSLGPDWDDKELEDLASHPVDQHLIQLGRIHKPDHGYSVKFVKSFINSIRHGINKYPPVNLKAKCNRLGSRDLKPPPRQFRSFVPGPQKANLKDHTAEAAKLFQDKKRLSSMLKGGRATISSLSRSTRYAFKQGKEAIKATSKLGKRSA

Target ORF information:

RefSeq Version	NM_001102607.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type VI, alpha 6 (Col6a6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102607.1

ORF Insert Sequence:

ATGCTGCTGG TTTTGTGCCT GACAATGATT TGTTTCCACG TGTGTGTGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGACGT GGTGTTTCTG GTGGACAGCT CCGATCACCT AGGGCTTAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAC AAGATGATCA GCAGCCTCCC CATAGAGGCC 
AACAAGTACC GCGTGGCCCT GGCCCAGTAC AGCGATGCTC TCCACAATGA GTTCCAGCTG 
GGCACCTTCA AGAACAGGAA CCCCATGCTG AACCACCTGA AGAAGAACTT CGGGTTCATC 
GGTGGCTCCC TGAAGATAGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAATG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCA 
GAGTCTGAGG ATGATGTGGA AGAGGCTGCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAAACCTGA AGGCGATGGC CACCTCTCAG 
TTCCATTTCA ATCTCAGGAC TGCCAGAGAC CTCAGCGTGT TTGCCCCAAA CATGACAGAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GGAATGGCAG ATGACATTAT TGTAGAAGCC 
TGCCAAGGCC CTTCTGTGGC TGATGTGGTG TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAGGAGGACC TAGATCATCT TAAAGCATTC CTGGGCGAAA GCATCTCTGC CCTGGACATA 
AAGGAAAATT GCATGAGGGT TGGCCTGGTG ACCTATAGCA ATGAGACAAG GGTGATCAGC 
TCTCTGAGCA CGGGTAACAA CAAGACAGAA GTCTTGCAGC GCATACAGGA TCTGTCCCCT 
CAAGTAGGGC AGGCCTACAC TGGAGCTGCC CTCAGAAAGA CTAGGAAGGA AATCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTCCCTCAGA TCGCTGTGCT GGTGACCCAC 
AGAGCATCAG AAGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
ATCTTTACCA TGGGCATAGA GGGGGCTAAC CCAGACGAGC TGGAGAAGAT CGCATCCCAC 
CCTGCGGAGC AGTTCACCTC CAAACTGGGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAACTGCG GAACCAAATC ACACACACGG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCTGC CTGTGTGGAC ACAGAGGAAG CCGATATCTA TCTACTCATT 
GATGGTTCAG GGAGCACCCA GCCCACAGAC TTCCATGAAA TGAAGACCTT CCTGTCAGAG 
GTGGTAGGCA TGTTCAACAT TGCTCCCCAC AAGGTGCGAG TAGGGGCCGT GCAGTACGCC 
GACACCTGGG ACTTGGAATT TGAGATCTCT AAGTATAGTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGAGA ATATCAGGCA GATGGGTGGG AATACCAACA CAGGGGCGGC TTTGAACTTC 
ACACTGAAGC TGTTGCAAAG AGCAAAGAAG GAACGAGGAA GCAAAGTGCC GTGTCACCTG 
GTGGTTCTGA CCAATGGCAT GTCTCGGGAC AGCGTCCTGG GGCCTGCCCA TAAGCTGAGA 
GAGGAAAACA TCAGAGTGCA TGCGATCGGT GTCAAGGAAG CCAACCAAAC GCAGCTTCGG 
GAGATAGCGG GAGAGGAAAA GCGAGTTTAC TACGTCCATG AGTTCGATGC CTTGAGGAAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA CATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATCGGAC CTGAAAACTT CAGCAAGATG 
AAGATGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGCGTGGTCC AGTTCAGCCA CGAAAACAAG GAGGAGTTTC AGCTCAACAC GTTCATGTCT 
CAAAGTGACA TCGCCAACGC CATTGACCGA ATGACTCACA TTGGAGAAAC AACCTTGACG 
GGCAGTGCCC TGACCTTTGT GTCTCAGTAC TTCAGTCCCG ATAAGGGGGC CAGGCCCAAT 
GTCAGGAAGT TCCTCATTCT TATCACGGAT GGTGAGGCTC AGGACATAGT AAGGGACCCA 
GCGATCGCCC TTCGAAAAGA AGGTGTGATT ATCTATTCTG TGGGAGTATT CGGCTCCAAT 
GTCACCCAGC TTGAGGAGAT CAGTGGAAAG CCAGAGATGG TTTTCTATGT TGAGAATTTT 
GACATTCTGC AGCATATCGA AGATGACCTC GTTCTGGGGA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTGGTG TTTGTCATCG ATAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCCCTG AAGTATGCTG ATGACCCCGA AGTGCTGTTT 
TACCTGGATG AACTAGGCAC GAAGCTGGAG GTAGTTTCAG TGCTCCAGAA TGACCATCCC 
ATGGGTGGAA ATACTTACAC CGCTGAGGCC CTCGCCTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACCGACGGG 
GAATCTCATG ACGCAGAGAA GCTCAACACC ACCGCCAAGG CCCTGAGAGA CAAAGGCATT 
CTCGTCCTGG CTGTGGGGAT TGCCGGTGCC AACAGCTGGG AGCTCTTGGC CATGGCAGGG 
TCAAGCGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGAAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTTGATTGTG AAATTGAAAA GGTTGACCTT 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGGG 
TTTTTGGTGT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGCGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACGTTCAC CGGGGAGAGG 
GAGATATCCA CCCAGATTGA GGGCATCCAG CAGATCTTTG GATACACCCA CATCGGAGAT 
GCTCTCAGGA AGGTGAAGTA TTACTTTCAG CCAGACATGG GCAGCAGGAT CAACGCAGGT 
ACCCCCCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGACGAGGT AGCTCAGGCC 
GCCGAGGAGC TGAGACACAA AGGTGTGGAC ATCTACTCGG TGGGCATCGG GGATGTGGAT 
GACCAGGAAT TGGTCCAGAT CACGGGGACC GCGGAGAAAA AACTGACCGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGGAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGCT TTGTGGATGT CGTGGTTGGA TTTGACATCT CAAGCCTGCA GAGAGGGCAG 
ACTCTGCTCG AAGGTCAGCC TTGGATGGGA TCCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAAGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ACGCTATGGA AAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTAAGCAGCC TGCAGGGTGT GACCGTGAAT GGCCCGTCTC GCCTCAACGC CAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAATAAG TCAGCTGCTC GTGGGAAGGT GGTCCTTCTA 
TTTTCAGATG GACTGGATGA TGGCATTGAG AAACTTGAAC AGAAGTCTGA TGAACTCAGG 
AAGGAAGGCC TGAATGCCCT CATAACCATT GCTGTGGATG GAGCTGCCGA TTCCAGTGAC 
CTGGCGGACC TCCTCTACAT TGAATTTGGG AAAGGATTTG AATACAGGAC ACAGTTCACA 
ATTGGAATGA GGAACCTTGG GAGTCAGCTG TCAAGGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTCTGCTCTG CAAGTGCACA GGAGGGGACG GCGCCATGGG GGATCCAGGC 
TCAGCAGGAA AAAAGGGACC CCCAGGATTC AAAGGCAGTG ATGGCTACCT CGGAGAGGAG 
GGCATTGCTG GAGAAAGAGG AGCCTCTGGG CCAATGGGAG AGCAAGGCAC GAAGGGATGC 
TTCGGTGCCA AAGGGCCTAA GGGAACCAGA GGACTCAGTG GAGAAGAGGG TGAAGTCGGG 
GAAGATGGGC TTGATGGATT AGATGGAGAA CAGGGTGACC ATGGGATTCC TGGAAGAAGA 
GGAGAAAAGG GTGACGAGGG ATCTCAGGGA AACCCAGGCA GGAGAGGGGC TGCCGGTGAC 
CGTGGAGCAA AGGGACTGCG AGGAGATCCG GGAACTCCTG GACGTGACAG TAGCATACAA 
GGACCCAAGG GCTTGAAAGG AGACCTTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGAACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACACATAGGC 
CCACAGGGAA ATCCAGGAAC CCCGGGCCCA GATGGACTTG CAGGTTCGCC AGGACTTAGA 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAAG TCTGGGAATG 
AAAGGTCCTC AGGGGCCTCC AGGACCCGGA GGACAAGCTG GGAGCCAAGG CCATTTGGGA 
AGCCAAGGAA ACAAAGGAGA ACCTGGAGAC CTGGGAGAAA AGGGAGCTGC TGGCTTTCCA 
GGTCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCGGGAT ATGGTAGCAT TGGCCGCAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC TGAAGGGTGA TATTGGGGAC 
CCTGGTGATC CAGGAGAAGC TGGTCCCAAG GGAGCCAGAG GCAAAACGGT ATCTGCTGGG 
ATTCCAGGAG AGCCGGGGTC CCCCGGGGAG CCAGGACCCC CTGGACGGAA GGGTGTGAAA 
GGGGCCCGAG GACTGGCTTC GTTTTCCACA TGTGACCTCA TTCAGTACGT GCGGGACCAC 
AGTCCTGGCA GACATGGAAA ACCTGAGTGC CCAGTGCATC CAACTGAGTT GGTGTTTGTC 
CTGGACCAGT CCCGGGATGT CACAGAGCAG GACTTTGAGC GAATGAAGGG GATGATGGTC 
TCCCTAGTTA GGGATGTCAA GGTTAGGGAG GCCAACTGCC CCGTGGGTGC CCGCGTTGCC 
ATCCTAGCCT ACAACTCCCA CACCAGGCAC CTCATCCGCT TCTCAGATGC CTACAGGAAG 
GACCAGCTGC TCACGGCGAT TAAAGCTCTT CCTTATGAGA GATCCTCCGA CAGCAGGGAG 
ATTGGCAAAG CCATGAGGTT CATCTCCAGG AATGTCTTCA AGAGAACCCT TCCAGGGGCT 
CATGTGAGAA GAATCGCCAC GTTCTTTAGC AGTGGTCCAT CGGCCGACGC CCAGACCATT 
ACCACAGCCG CCATGGAGTT CAGTGCCCTG GACATTGTAC CTGTGGTGAT TGCGTTCAGC 
AACGTGCCCT CGGTCAAGCG CGCCTTTTCA ATTGATGACA CTGGCACATT CCAAGTCATT 
GTGGTTCCCT CCGGGTCTGA TGAAGGGCCA GCGTTAGAGA GACTCCAGCG GTGCACTTTC 
TGCTATGATC TATGCAAGCC GGATGCTTCG TGTGACCAAG CCAAACCACC CCCCATTCAG 
TCCTACCTGG ACACTGCATT CCTACTGGAT GGCTCTCGGC ACGTGGGAAG TGCAGAGTTT 
GAAGACATGA GAGACTTTCT AGAGGCACTG CTAGATCACT TCGAAATCAC TTCGGAGCCA 
GAGACATCTG TCACTGGAGA CAGGGTGGCC CTGCTGAGCC ATGCCCCCCT CGACTTCCTA 
CCCAACACCC AGAGGAGTCC GGTTAGAACT GAGTTCAACC TAACCAGCTA TAGCAGTAAG 
CGCCTCATGA AGAGACACGT GGATCAGGCA GTCCAGCAGC TGCATGGAGA TGCTTTTCTT 
GGCCATGCCC TCGGGTGGGC ACTGGACAAC GTCTTTTTAA ACACCCCCAA TTTGAGACGA 
AACAAAGTCA TATTTGTGAT ATCTGCTGGG GAAACCAGCC ACCTGGATGC GGAAACCTTA 
AAGAAAGAGT CCCTACGAGC CAAATGTCAT GGGTATGCCC TCTTTGTCTT TTCCTTGGGC 
CCTGACTGGG ATGACAAGGA ACTGGAAGAC CTGGCCAGCC ACCCCGTAGA CCAGCACTTG 
ATCCAGCTTG GCCGAATTCA TAAACCTGAC CACGGATACA GTGTGAAGTT TGTGAAGTCT 
TTTATAAACT CCATCAGGCA TGGGATAAAC AAGTATCCAC CGGTCAACCT CAAAGCCAAG 
TGCAACAGAC TCGGCTCTAG GGATCTGAAG CCGCCCCCAC GCCAGTTTCG AAGCTTTGTT 
CCAGGACCAC AGAAAGCCAA CCTCAAAGAC CACACAGCAG AGGCAGCAAA GCTCTTTCAA 
GATAAAAAAC GCCTTTCAAG TATGCTGAAA GGGGGCAGAG CTACTATTTC AAGTCTTTCC 
AGAAGCACAC GCTATGCCTT TAAACAGGGG AAAGAAGCAA TAAAAGCCAC TTCTAAACTC 
GGTAAACGAA GTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

Regulatory mechanisms of anthrax toxin receptor 1-dependent vascular and connective tissue homeostasis.
Matrix biology : journal of the International Society for Matrix Biology4256-73(2015 Mar)
Besschetnova TY,Ichimura T,Katebi N,St Croix B,Bonventre JV,Olsen BR

A mouse model for dominant collagen VI disorders: heterozygous deletion of Col6a3 Exon 16.
The Journal of biological chemistry289(15)10293-307(2014 Apr)
Pan TC,Zhang RZ,Arita M,Bogdanovich S,Adams SM,Gara SK,Wagener R,Khurana TS,Birk DE,Chu ML

Differential and restricted expression of novel collagen VI chains in mouse.
Matrix biology : journal of the International Society for Matrix Biology30(4)248-57(2011 May)
Gara SK,Grumati P,Squarzoni S,Sabatelli P,Urciuolo A,Bonaldo P,Paulsson M,Wagener R

Three novel collagen VI chains, alpha4(VI), alpha5(VI), and alpha6(VI).
The Journal of biological chemistry283(29)20170-80(2008 Jul)
Fitzgerald J,Rich C,Zhou FH,Hansen U

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Col6a6 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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