Col6a6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col6a6
Entrez Gene ID	315979
Full Name	collagen type VI alpha 6 chain
Synonyms	RGD1309172
General protein information	Preferred Names collagen type VI alpha 6 chain Names collagen alpha-6(VI) chain collagen type VI alpha 6 chain collagen, type VI, alpha 6
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32

mRNA and Protein(s)

mRNA	Protein	Name
XM_017596190.1	XP_017451679.1	collagen alpha-6(VI) chain isoform X1
XM_008757862.2	XP_008756084.1	collagen alpha-6(VI) chain isoform X2
XM_017603711.1	XP_017459200.1	collagen alpha-6(VI) chain isoform X4
XM_017603712.1	XP_017459201.1	collagen alpha-6(VI) chain isoform X5
XM_002727098.5	XP_002727144.1	collagen alpha-6(VI) chain isoform X2
XM_008757863.2	XP_008756085.1	collagen alpha-6(VI) chain isoform X2
XM_006226549.3	XP_006226611.1	collagen alpha-6(VI) chain isoform X2
XM_017603710.1	XP_017459199.1	collagen alpha-6(VI) chain isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL6A6	XP_003310057.1
Bos taurus (cattle)	COL6A6	XP_002685218.2
Gallus gallus (chicken)	COL6A6	XP_426008.4
Xenopus tropicalis (tropical clawed frog)	LOC100486063	XP_002942405.2
Canis lupus familiaris (dog)	COL6A6	XP_853279.3
Mus musculus (house mouse)	Col6a6	NP_001096077.1
	COL6A6	XP_001113364.2
Homo sapiens (human)	COL6A6	NP_001096078.1
Rattus norvegicus (Norway rat)	Col6a6	XP_236593.5
Danio rerio (zebrafish)	col6a6	XP_692457.6

Gene Ontology

The following Col6a6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col6a6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa55951	XM_017603712.1 Latest version!	Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa55949	XM_017603710.1 Latest version!	Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa55950	XM_017603711.1 Latest version!	Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa62615	XM_017596190.1 Latest version!	Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa18100	XM_002727098.5 Latest version!	Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18100	XM_008757863.2 Latest version!	Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18100	XM_006226549.3 Latest version!	Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18100	XM_008757862.2 Latest version!	Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col6a6 ( XM_017603712.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017459201.1 Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ORa55951
Clone ID Related Accession (Same CDS sequence)	XM_017603712.1
Accession Version	XM_017603712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-6(VI) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000076.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGA TTTTGTTCCT GATGATGATT TGTTTACACG TTTGTGCGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGATGT GGTTTTTCTG GTGGACAGTT CCGATCACCT AGGGATGAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAA AAGCTAGTCA GCAGCCTCCC CGTAGAGGCC 
AACAAGTACC GTGTGGCCCT GGCCCAGTAC AGTGATGCTC TCCACAATGA GTTCCACCTA 
GGCGCCTTCA AGAACAGGAA CCCCATGCTG AACCACCTCA AGAAGAACTT CGGGTTCATC 
GGCGGCTCCC TGAAGATTGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAACG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCG 
GAGTCCGAGG ATGACGTAGA AGAGGCTTCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAGACCTGA AGGCCATGGC CACCTCTCAG 
TTTCATTTCA ACCTCAGGAC TGCCAGAGAC CTCAGCATGT TTGCCCCAAA CATGACACAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GCAATGACTG ATGACATTAT TGTAGAAGCT 
TGCCAAGGCC CATCTGTGGC CGATGTGGTA TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAAGAGAACC TAGATCATCT TAAAGCATTC CTGGGGGAAA GTGTCTCTGC CCTTGACATA 
AAGGAAAACT GCATGAGGGT TGGCCTGGTG GCCTATAGCA ACGAAACAAG GGTGATCAGT 
TCTCTGAGCA TGGGTGTCAA CAAGACGGAG GTCTTACAGC GCATACAGGA TCTGTCCCCT 
CACGTAGGGC AGGCCTACAC AGGAGCTGCC CTCAGAAAGA CGAGGAAGGA AGTCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTGCCTCAGA TTGCTGTGCT GGTGACCCAC 
AGAGCTTCAG ATGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
GTCTTTACCA TGGGCGTAGA GGGGGCTAAC CCAGAACAGC TGGAGAAGAT CGCATCCTAC 
CCTGCCGAGC AGTTCACCTC CAAACTGAGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAGCTGAG GAACCAAATC ACACACACAG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCAGC CTGTGTGGAC ACAGAGGAAG CGGATATCTA TCTGCTCATT 
GATGGTTCAG GGAACACGCA GCCCACAGAC TTCCATGAGA TGAAGATCTT CCTGTCCGAG 
GTGGTGGACA TGTTCAACAT CGCTCCCCAC AAGGTGCGAG TCGGGGCCGT GCAGTACGCC 
GACACCTGGG ACCTGGAATT TGAGATCTCT AAGTATACTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGACA ACATCAGGCA GATGGGTGGA AACACCAATA CGGGTGCAGC CCTGAACTTC 
ACCCTGACGC TGTTGCAAAG AGCAAAGAAG CAACGGGGAA ACAAAGTGCC ATGTCACCTG 
GTTGTCCTGA CAAACGGCAT GTCCCAGGAC AGCGTCCTGG GTCCCGCGCA TAAGCTGAGG 
GAGGAAAACA TCCGTGTGCA TGCGATCGGC GTCAAGGAAG CCAACCAAAC ACAGCTGCGG 
GAGATAGCGG GAGATGAAAA GCGAGTTTAT TACGTGCATG AATTCGATGC CTTGAGGGAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA TATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATTGGAC TCGAAAACTT CAGCAAGATG 
AAGGTGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGTGTGGTCC AGTTCAGCCA CGAAAACAGG GAGGAGTTTC AGCTCAACAC ATTCATGTCT 
CAAAATGACA TTGCCAACGC CATCGACCAA ATGGCTCACA TTGGAGAGAC AACCTTGACA 
GGCAGTGCGC TGACCTTTGT GTCTCAGTAC TTCAGTCCTG AAAAGGGGGC CCGGCCCAAT 
GTTAGGAAGT TCCTCATTCT CATCACGGAT GGCGAGGCTC AGGACATAGT AAAGGACCCA 
GCAGTAGCCC TTCGAAAAGA CGGTGTGATT ATCTATTCTG TGGGAGTGTT CGGCTCCAAT 
GTCACCCAGC TCGAGGAGAT CAGTGGGAAG CCGGAGATGG TTTTCTATGT TGAGAATTTT 
GACATCCTAC AGCATATTGA AGATGATCTC GTTCTGGGAA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTAGTG TTTGTCATCG ACAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCTCTG AAATATGCTG ATGACCCAGA GGTGCTGTTT 
TACCTGGATG AACTGGGCAC AAAGCTGGAG GTGATTTCAG TGCTCCAGAA TGACCAGCCC 
ATGGGTGGTA ACACTTACAC TGCTGAGGCC CTGGCTTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACTGATGGA 
GAATCTCATG ATGCAGAGAA GCTCAATGCC ACCGCCAAGG CCCTGAGGGA CAAAGGCATT 
CTGGTCCTAG CTGTGGGGAT TGCTGGCGCC AATACCTGGG AGCTCTTGGC CATGGCTGGG 
TCGAGTGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGGAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTCGATTGCG AAATTGAAAA AGTTGACCTC 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGAG 
TTTTTGGCAT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGAGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACATTCAC AGGGGAAAAG 
GAGATATCCA CACAGATTGA GGCCATCCAG CAGATCTTTG GTTACACCCA CATTGGAGAT 
GCACTCAGGA AGGTGAAGCA TTACTTTCGG CCAGACACGG GCAGCAGGAT CAACGCCGGT 
ACCCCTCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGATGAGGT GGCCCAGGCG 
GCTGAGGAGC TGAGACACAA GGGTGTGGAC ATCTACTCGG TGGGCATCGG GGACGTGGAT 
GACCAGCAGC TGATCCAGAT AACCGGGACA GCGGAGAAAA AACTGACGGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGTAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGTT TCGTGGATGT GGTGGTTGGA TTTGATATCT CAAGTCAGCG GAGAGGGCAG 
ACTTTGCTCG AAGGTCAGCC TTGGATGGGG ACCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAGGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ATGCTATGGA GAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTGAGCAGCC TGCAGGGTGT GACAGTGAAC GGCCCATCTC GCCTCAACCC AAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAACAAG TCCGCTGCTC GAGGGAAGGT GGTCCTTCTG 
TTTTCAGATG GATTGGATGA CGGCGTTGAA AAACTTGAAC AGAAATCTGA TGAACTCAGA 
AAGGAAGCGA CTGAAGACGT TGCACTGAAG GTTGTGACTC ATGTAGTGAG TCTGGAGGGA 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017459201.1
CDS	38..4063
Translation	MLLILFLMMICLHVCANQDSGPEYADVVFLVDSSDHLGMKSFPLVKTFIQKLVSSLPVEANKYRVALAQYSDALHNEFHLGAFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEASKALREDGVKIISVGVQKASEEDLKAMATSQFHFNLRTARDLSMFAPNMTQIIKDVTQYREAMTDDIIVEACQGPSVADVVFLLDMAINGSQENLDHLKAFLGESVSALDIKENCMRVGLVAYSNETRVISSLSMGVNKTEVLQRIQDLSPHVGQAYTGAALRKTRKEVFSAQRGSRKNQGVPQIAVLVTHRASDDNVTKAAVNLRREGVTVFTMGVEGANPEQLEKIASYPAEQFTSKLSNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGNTQPTDFHEMKIFLSEVVDMFNIAPHKVRVGAVQYADTWDLEFEISKYTNKPDLGKAIDNIRQMGGNTNTGAALNFTLTLLQRAKKQRGNKVPCHLVVLTNGMSQDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGDEKRVYYVHEFDALRDIRNQVVQEICAEEACRDMKADIMFLVDSSGSIGLENFSKMKVFMKNLVSKSQIGADRVQIGVVQFSHENREEFQLNTFMSQNDIANAIDQMAHIGETTLTGSALTFVSQYFSPEKGARPNVRKFLILITDGEAQDIVKDPAVALRKDGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVISVLQNDQPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNATAKALRDKGILVLAVGIAGANTWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFQKMKEFLASVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEKEISTQIEAIQQIFGYTHIGDALRKVKHYFRPDTGSRINAGTPQVLLVLTDGRSQDEVAQAAEELRHKGVDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVKKRIVRNICTSGGESNCFVDVVVGFDISSQRRGQTLLEGQPWMGTYLQDLLRAISSLNGVSCEVGTETQVSIAFQVTNAMERYPSKFEIYSENILSSLQGVTVNGPSRLNPNLLSSLWDTFQNKSAARGKVVLLFSDGLDDGVEKLEQKSDELRKEATEDVALKVVTHVVSLEGA

Target ORF information:

RefSeq Version	XM_017603712.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017603712.1

ORF Insert Sequence:

ATGCTGCTGA TTTTGTTCCT GATGATGATT TGTTTACACG TTTGTGCGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGATGT GGTTTTTCTG GTGGACAGTT CCGATCACCT AGGGATGAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAA AAGCTAGTCA GCAGCCTCCC CGTAGAGGCC 
AACAAGTACC GTGTGGCCCT GGCCCAGTAC AGTGATGCTC TCCACAATGA GTTCCACCTA 
GGCGCCTTCA AGAACAGGAA CCCCATGCTG AACCACCTCA AGAAGAACTT CGGGTTCATC 
GGCGGCTCCC TGAAGATTGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAACG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCG 
GAGTCCGAGG ATGACGTAGA AGAGGCTTCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAGACCTGA AGGCCATGGC CACCTCTCAG 
TTTCATTTCA ACCTCAGGAC TGCCAGAGAC CTCAGCATGT TTGCCCCAAA CATGACACAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GCAATGACTG ATGACATTAT TGTAGAAGCT 
TGCCAAGGCC CATCTGTGGC CGATGTGGTA TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAAGAGAACC TAGATCATCT TAAAGCATTC CTGGGGGAAA GTGTCTCTGC CCTTGACATA 
AAGGAAAACT GCATGAGGGT TGGCCTGGTG GCCTATAGCA ACGAAACAAG GGTGATCAGT 
TCTCTGAGCA TGGGTGTCAA CAAGACGGAG GTCTTACAGC GCATACAGGA TCTGTCCCCT 
CACGTAGGGC AGGCCTACAC AGGAGCTGCC CTCAGAAAGA CGAGGAAGGA AGTCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTGCCTCAGA TTGCTGTGCT GGTGACCCAC 
AGAGCTTCAG ATGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
GTCTTTACCA TGGGCGTAGA GGGGGCTAAC CCAGAACAGC TGGAGAAGAT CGCATCCTAC 
CCTGCCGAGC AGTTCACCTC CAAACTGAGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAGCTGAG GAACCAAATC ACACACACAG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCAGC CTGTGTGGAC ACAGAGGAAG CGGATATCTA TCTGCTCATT 
GATGGTTCAG GGAACACGCA GCCCACAGAC TTCCATGAGA TGAAGATCTT CCTGTCCGAG 
GTGGTGGACA TGTTCAACAT CGCTCCCCAC AAGGTGCGAG TCGGGGCCGT GCAGTACGCC 
GACACCTGGG ACCTGGAATT TGAGATCTCT AAGTATACTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGACA ACATCAGGCA GATGGGTGGA AACACCAATA CGGGTGCAGC CCTGAACTTC 
ACCCTGACGC TGTTGCAAAG AGCAAAGAAG CAACGGGGAA ACAAAGTGCC ATGTCACCTG 
GTTGTCCTGA CAAACGGCAT GTCCCAGGAC AGCGTCCTGG GTCCCGCGCA TAAGCTGAGG 
GAGGAAAACA TCCGTGTGCA TGCGATCGGC GTCAAGGAAG CCAACCAAAC ACAGCTGCGG 
GAGATAGCGG GAGATGAAAA GCGAGTTTAT TACGTGCATG AATTCGATGC CTTGAGGGAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA TATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATTGGAC TCGAAAACTT CAGCAAGATG 
AAGGTGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGTGTGGTCC AGTTCAGCCA CGAAAACAGG GAGGAGTTTC AGCTCAACAC ATTCATGTCT 
CAAAATGACA TTGCCAACGC CATCGACCAA ATGGCTCACA TTGGAGAGAC AACCTTGACA 
GGCAGTGCGC TGACCTTTGT GTCTCAGTAC TTCAGTCCTG AAAAGGGGGC CCGGCCCAAT 
GTTAGGAAGT TCCTCATTCT CATCACGGAT GGCGAGGCTC AGGACATAGT AAAGGACCCA 
GCAGTAGCCC TTCGAAAAGA CGGTGTGATT ATCTATTCTG TGGGAGTGTT CGGCTCCAAT 
GTCACCCAGC TCGAGGAGAT CAGTGGGAAG CCGGAGATGG TTTTCTATGT TGAGAATTTT 
GACATCCTAC AGCATATTGA AGATGATCTC GTTCTGGGAA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTAGTG TTTGTCATCG ACAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCTCTG AAATATGCTG ATGACCCAGA GGTGCTGTTT 
TACCTGGATG AACTGGGCAC AAAGCTGGAG GTGATTTCAG TGCTCCAGAA TGACCAGCCC 
ATGGGTGGTA ACACTTACAC TGCTGAGGCC CTGGCTTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACTGATGGA 
GAATCTCATG ATGCAGAGAA GCTCAATGCC ACCGCCAAGG CCCTGAGGGA CAAAGGCATT 
CTGGTCCTAG CTGTGGGGAT TGCTGGCGCC AATACCTGGG AGCTCTTGGC CATGGCTGGG 
TCGAGTGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGGAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTCGATTGCG AAATTGAAAA AGTTGACCTC 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGAG 
TTTTTGGCAT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGAGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACATTCAC AGGGGAAAAG 
GAGATATCCA CACAGATTGA GGCCATCCAG CAGATCTTTG GTTACACCCA CATTGGAGAT 
GCACTCAGGA AGGTGAAGCA TTACTTTCGG CCAGACACGG GCAGCAGGAT CAACGCCGGT 
ACCCCTCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGATGAGGT GGCCCAGGCG 
GCTGAGGAGC TGAGACACAA GGGTGTGGAC ATCTACTCGG TGGGCATCGG GGACGTGGAT 
GACCAGCAGC TGATCCAGAT AACCGGGACA GCGGAGAAAA AACTGACGGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGTAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGTT TCGTGGATGT GGTGGTTGGA TTTGATATCT CAAGTCAGCG GAGAGGGCAG 
ACTTTGCTCG AAGGTCAGCC TTGGATGGGG ACCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAGGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ATGCTATGGA GAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTGAGCAGCC TGCAGGGTGT GACAGTGAAC GGCCCATCTC GCCTCAACCC AAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAACAAG TCCGCTGCTC GAGGGAAGGT GGTCCTTCTG 
TTTTCAGATG GATTGGATGA CGGCGTTGAA AAACTTGAAC AGAAATCTGA TGAACTCAGA 
AAGGAAGCGA CTGAAGACGT TGCACTGAAG GTTGTGACTC ATGTAGTGAG TCTGGAGGGA 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a6 ( XM_017603710.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017459199.1 Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa55949
Clone ID Related Accession (Same CDS sequence)	XM_017603710.1
Accession Version	XM_017603710.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000076.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGA TTTTGTTCCT GATGATGATT TGTTTACACG TTTGTGCGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGATGT GGTTTTTCTG GTGGACAGTT CCGATCACCT AGGGATGAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAA AAGCTAGTCA GCAGCCTCCC CGTAGAGGCC 
AACAAGTACC GTGTGGCCCT GGCCCAGTAC AGTGATGCTC TCCACAATGA GTTCCACCTA 
GGCGCCTTCA AGAACAGGAA CCCCATGCTG AACCACCTCA AGAAGAACTT CGGGTTCATC 
GGCGGCTCCC TGAAGATTGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAACG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCG 
GAGTCCGAGG ATGACGTAGA AGAGGCTTCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAGACCTGA AGGCCATGGC CACCTCTCAG 
TTTCATTTCA ACCTCAGGAC TGCCAGAGAC CTCAGCATGT TTGCCCCAAA CATGACACAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GCAATGACTG ATGACATTAT TGTAGAAGCT 
TGCCAAGGCC CATCTGTGGC CGATGTGGTA TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAAGAGAACC TAGATCATCT TAAAGCATTC CTGGGGGAAA GTGTCTCTGC CCTTGACATA 
AAGGAAAACT GCATGAGGGT TGGCCTGGTG GCCTATAGCA ACGAAACAAG GGTGATCAGT 
TCTCTGAGCA TGGGTGTCAA CAAGACGGAG GTCTTACAGC GCATACAGGA TCTGTCCCCT 
CACGTAGGGC AGGCCTACAC AGGAGCTGCC CTCAGAAAGA CGAGGAAGGA AGTCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTGCCTCAGA TTGCTGTGCT GGTGACCCAC 
AGAGCTTCAG ATGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
GTCTTTACCA TGGGCGTAGA GGGGGCTAAC CCAGAACAGC TGGAGAAGAT CGCATCCTAC 
CCTGCCGAGC AGTTCACCTC CAAACTGAGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAGCTGAG GAACCAAATC ACACACACAG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCAGC CTGTGTGGAC ACAGAGGAAG CGGATATCTA TCTGCTCATT 
GATGGTTCAG GGAACACGCA GCCCACAGAC TTCCATGAGA TGAAGATCTT CCTGTCCGAG 
GTGGTGGACA TGTTCAACAT CGCTCCCCAC AAGGTGCGAG TCGGGGCCGT GCAGTACGCC 
GACACCTGGG ACCTGGAATT TGAGATCTCT AAGTATACTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGACA ACATCAGGCA GATGGGTGGA AACACCAATA CGGGTGCAGC CCTGAACTTC 
ACCCTGACGC TGTTGCAAAG AGCAAAGAAG CAACGGGGAA ACAAAGTGCC ATGTCACCTG 
GTTGTCCTGA CAAACGGCAT GTCCCAGGAC AGCGTCCTGG GTCCCGCGCA TAAGCTGAGG 
GAGGAAAACA TCCGTGTGCA TGCGATCGGC GTCAAGGAAG CCAACCAAAC ACAGCTGCGG 
GAGATAGCGG GAGATGAAAA GCGAGTTTAT TACGTGCATG AATTCGATGC CTTGAGGGAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA TATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATTGGAC TCGAAAACTT CAGCAAGATG 
AAGGTGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGTGTGGTCC AGTTCAGCCA CGAAAACAGG GAGGAGTTTC AGCTCAACAC ATTCATGTCT 
CAAAATGACA TTGCCAACGC CATCGACCAA ATGGCTCACA TTGGAGAGAC AACCTTGACA 
GGCAGTGCGC TGACCTTTGT GTCTCAGTAC TTCAGTCCTG AAAAGGGGGC CCGGCCCAAT 
GTTAGGAAGT TCCTCATTCT CATCACGGAT GGCGAGGCTC AGGACATAGT AAAGGACCCA 
GCAGTAGCCC TTCGAAAAGA CGGTGTGATT ATCTATTCTG TGGGAGTGTT CGGCTCCAAT 
GTCACCCAGC TCGAGGAGAT CAGTGGGAAG CCGGAGATGG TTTTCTATGT TGAGAATTTT 
GACATCCTAC AGCATATTGA AGATGATCTC GTTCTGGGAA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTAGTG TTTGTCATCG ACAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCTCTG AAATATGCTG ATGACCCAGA GGTGCTGTTT 
TACCTGGATG AACTGGGCAC AAAGCTGGAG GTGATTTCAG TGCTCCAGAA TGACCAGCCC 
ATGGGTGGTA ACACTTACAC TGCTGAGGCC CTGGCTTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACTGATGGA 
GAATCTCATG ATGCAGAGAA GCTCAATGCC ACCGCCAAGG CCCTGAGGGA CAAAGGCATT 
CTGGTCCTAG CTGTGGGGAT TGCTGGCGCC AATACCTGGG AGCTCTTGGC CATGGCTGGG 
TCGAGTGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGGAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTCGATTGCG AAATTGAAAA AGTTGACCTC 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGAG 
TTTTTGGCAT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGAGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACATTCAC AGGGGAAAAG 
GAGATATCCA CACAGATTGA GGCCATCCAG CAGATCTTTG GTTACACCCA CATTGGAGAT 
GCACTCAGGA AGGTGAAGCA TTACTTTCGG CCAGACACGG GCAGCAGGAT CAACGCCGGT 
ACCCCTCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGATGAGGT GGCCCAGGCG 
GCTGAGGAGC TGAGACACAA GGGTGTGGAC ATCTACTCGG TGGGCATCGG GGACGTGGAT 
GACCAGCAGC TGATCCAGAT AACCGGGACA GCGGAGAAAA AACTGACGGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGTAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGTT TCGTGGATGT GGTGGTTGGA TTTGATATCT CAAGTCAGCG GAGAGGGCAG 
ACTTTGCTCG AAGGTCAGCC TTGGATGGGG ACCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAGGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ATGCTATGGA GAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTGAGCAGCC TGCAGGGTGT GACAGTGAAC GGCCCATCTC GCCTCAACCC AAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAACAAG TCCGCTGCTC GAGGGAAGGT GGTCCTTCTG 
TTTTCAGATG GATTGGATGA CGGCGTTGAA AAACTTGAAC AGAAATCTGA TGAACTCAGA 
AAGGAAGGCC TGAATGCCCT CATAACCATC GCTGTGGACG GAGCTGCCGA TTCCAGTGAC 
CTGGCTGACC TTCTCTACAT TGAATTTGGG AAAGGATTTG AGTACAGAAC ACAGTTCACA 
ATCGGAATGG GGAACCTTGG GAGTCACCTG TCAAAGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTTTGCTCTG CAAGTGCACA GGATGGGACG GCGCCATGGG GGATCCGGGC 
TCAGCAGGGG AAAAGGGACC CCCAGGATTT AAAGGCAGTG AAGGCTACCT GGGAGAAGAA 
GGCATTGCTG GAGAAAGAGG AGCCTTGGGA CCAATGGGAG AGCAAGGTAC AAAGGGATGC 
TTAGGTGCCA AAGGGCCCAA GGGAGCCCGA GGACTCAGCG GAGAAGAGGG TGAAGTTGGG 
GAAGATGGGC TTGACGGATT AAATGGAGAA CAGGGTGACA GTGGGATTCC TGGAAGAAGA 
GGAGAAAGGG GTGATGCGGG ATCTCAGGGA AGCCCAGGCA AGAGAGGGGC TTCTGGTGAC 
CGTGGACCGA AGGGCCTACG AGGAGATCCG GGAACTCCTG GACGTGATAA TAGCATACAG 
GGACCCAAGG GCTTGAAAGG AGACCCTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGGACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACATACAGGC 
CCACAGGGAA ATCCAGGCAT CACAGGCCCA GATGGACTTG AAGGTTCGCC CGGACTTAAG 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAGG CTTCGGAATG 
AAAGGTCCCC AGGGGCCTCC AGGACCCAAA GGACGAGCTG GGAACCAAGG CCATTGGGGA 
AGCCAAGGAA GCAAAGGAGA ACCTGGAGAC CTGGGGGAAA AGGGAGCTGC TGGCTTTCCA 
GGGCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCAGGAT ATGGTAGCAT TGGCCGAAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC CAAAGGGTGA TATTGGGGAC 
CCTGGCGATC CAGGAGAGGC TGGTCCCAAG GGAGCCAGAG GCAAAACGGT ATCTGCTGGG 
ATTCCAGGAG AGCCAGGATC CCCCGGGGAG CCAGGACCCC CTGGAAGGAA GGGCATGAAA 
GGAGCCCGAG GACTGGCTTC ATTTTCCACA TGTGACCTCA TTCAGTATGT GCGGGAACAC 
AGTCCTGTCA GACACGGAAA ACCTGAGTGC CCAGTGCATC CAACTGAGTT GGTGTTTGTC 
CTGGACCAGT CCCGGGATGT CACAGAACAG GACTTTGAGC GGATGAAGGG GATGATGGCC 
TCCTTAGTTA GGGACATCAA AGTTAGGGAT GCCAGCTGCC CCGTGGGTGC CCGCGTTGCC 
ATCCTAGCCT ACAACTCCCA CACCAGGCAC CTCATCCGCT TCTCGGATGC CTACAGGAAG 
GACCAGCTGC TCAGGGAAAT TAAAGCTCTT CCTTACGAGA AATCCTCCGA CAGCAGGGAG 
ATTGGCAAAG CCATGAGGTT TATCTCCAGG AATGTCTTCA AGAGAACCCT CCCAGGGGCT 
CACGTGAGAA GAATCGCCAC GTTCTTTAGC AGTGGCCAGT CAGCCGATGT CCAGTCCATT 
ACCACAGCTG CCATGGAGTT CAGTGCCCTG GACATCATAC CAGTGGTGAT TGCATTCAGC 
AACGTGCCCT CGGTCAAGCG TGCCTTTTCG ACCTATGCAA GCCAGATGCT TCTTGTGACC 
AAGCCAAACC CCCCATTCAG TCCTACCTGG ATGCTGCATT CCTGCTGGAT AGCTCCTGGC 
ATGTGGGAGG TGCAGAATTT GAAGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017459199.1
CDS	38..5887
Translation	MLLILFLMMICLHVCANQDSGPEYADVVFLVDSSDHLGMKSFPLVKTFIQKLVSSLPVEANKYRVALAQYSDALHNEFHLGAFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEASKALREDGVKIISVGVQKASEEDLKAMATSQFHFNLRTARDLSMFAPNMTQIIKDVTQYREAMTDDIIVEACQGPSVADVVFLLDMAINGSQENLDHLKAFLGESVSALDIKENCMRVGLVAYSNETRVISSLSMGVNKTEVLQRIQDLSPHVGQAYTGAALRKTRKEVFSAQRGSRKNQGVPQIAVLVTHRASDDNVTKAAVNLRREGVTVFTMGVEGANPEQLEKIASYPAEQFTSKLSNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGNTQPTDFHEMKIFLSEVVDMFNIAPHKVRVGAVQYADTWDLEFEISKYTNKPDLGKAIDNIRQMGGNTNTGAALNFTLTLLQRAKKQRGNKVPCHLVVLTNGMSQDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGDEKRVYYVHEFDALRDIRNQVVQEICAEEACRDMKADIMFLVDSSGSIGLENFSKMKVFMKNLVSKSQIGADRVQIGVVQFSHENREEFQLNTFMSQNDIANAIDQMAHIGETTLTGSALTFVSQYFSPEKGARPNVRKFLILITDGEAQDIVKDPAVALRKDGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVISVLQNDQPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNATAKALRDKGILVLAVGIAGANTWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFQKMKEFLASVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEKEISTQIEAIQQIFGYTHIGDALRKVKHYFRPDTGSRINAGTPQVLLVLTDGRSQDEVAQAAEELRHKGVDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVKKRIVRNICTSGGESNCFVDVVVGFDISSQRRGQTLLEGQPWMGTYLQDLLRAISSLNGVSCEVGTETQVSIAFQVTNAMERYPSKFEIYSENILSSLQGVTVNGPSRLNPNLLSSLWDTFQNKSAARGKVVLLFSDGLDDGVEKLEQKSDELRKEGLNALITIAVDGAADSSDLADLLYIEFGKGFEYRTQFTIGMGNLGSHLSKQLINVAERTCCCLLCKCTGWDGAMGDPGSAGEKGPPGFKGSEGYLGEEGIAGERGALGPMGEQGTKGCLGAKGPKGARGLSGEEGEVGEDGLDGLNGEQGDSGIPGRRGERGDAGSQGSPGKRGASGDRGPKGLRGDPGTPGRDNSIQGPKGLKGDPGRQGRRGWPGSPGTPGSRRKMVVHGRRGHTGPQGNPGITGPDGLEGSPGLKGPQGPRGEVGEKGEKGGFGMKGPQGPPGPKGRAGNQGHWGSQGSKGEPGDLGEKGAAGFPGPRGLQGDDGSPGYGSIGRKGTKGQEGFPGESGPKGDIGDPGDPGEAGPKGARGKTVSAGIPGEPGSPGEPGPPGRKGMKGARGLASFSTCDLIQYVREHSPVRHGKPECPVHPTELVFVLDQSRDVTEQDFERMKGMMASLVRDIKVRDASCPVGARVAILAYNSHTRHLIRFSDAYRKDQLLREIKALPYEKSSDSREIGKAMRFISRNVFKRTLPGAHVRRIATFFSSGQSADVQSITTAAMEFSALDIIPVVIAFSNVPSVKRAFSTYASQMLLVTKPNPPFSPTWMLHSCWIAPGMWEVQNLKT

Target ORF information:

RefSeq Version	XM_017603710.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017603710.1

ORF Insert Sequence:

ATGCTGCTGA TTTTGTTCCT GATGATGATT TGTTTACACG TTTGTGCGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGATGT GGTTTTTCTG GTGGACAGTT CCGATCACCT AGGGATGAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAA AAGCTAGTCA GCAGCCTCCC CGTAGAGGCC 
AACAAGTACC GTGTGGCCCT GGCCCAGTAC AGTGATGCTC TCCACAATGA GTTCCACCTA 
GGCGCCTTCA AGAACAGGAA CCCCATGCTG AACCACCTCA AGAAGAACTT CGGGTTCATC 
GGCGGCTCCC TGAAGATTGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAACG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCG 
GAGTCCGAGG ATGACGTAGA AGAGGCTTCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAGACCTGA AGGCCATGGC CACCTCTCAG 
TTTCATTTCA ACCTCAGGAC TGCCAGAGAC CTCAGCATGT TTGCCCCAAA CATGACACAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GCAATGACTG ATGACATTAT TGTAGAAGCT 
TGCCAAGGCC CATCTGTGGC CGATGTGGTA TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAAGAGAACC TAGATCATCT TAAAGCATTC CTGGGGGAAA GTGTCTCTGC CCTTGACATA 
AAGGAAAACT GCATGAGGGT TGGCCTGGTG GCCTATAGCA ACGAAACAAG GGTGATCAGT 
TCTCTGAGCA TGGGTGTCAA CAAGACGGAG GTCTTACAGC GCATACAGGA TCTGTCCCCT 
CACGTAGGGC AGGCCTACAC AGGAGCTGCC CTCAGAAAGA CGAGGAAGGA AGTCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTGCCTCAGA TTGCTGTGCT GGTGACCCAC 
AGAGCTTCAG ATGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
GTCTTTACCA TGGGCGTAGA GGGGGCTAAC CCAGAACAGC TGGAGAAGAT CGCATCCTAC 
CCTGCCGAGC AGTTCACCTC CAAACTGAGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAGCTGAG GAACCAAATC ACACACACAG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCAGC CTGTGTGGAC ACAGAGGAAG CGGATATCTA TCTGCTCATT 
GATGGTTCAG GGAACACGCA GCCCACAGAC TTCCATGAGA TGAAGATCTT CCTGTCCGAG 
GTGGTGGACA TGTTCAACAT CGCTCCCCAC AAGGTGCGAG TCGGGGCCGT GCAGTACGCC 
GACACCTGGG ACCTGGAATT TGAGATCTCT AAGTATACTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGACA ACATCAGGCA GATGGGTGGA AACACCAATA CGGGTGCAGC CCTGAACTTC 
ACCCTGACGC TGTTGCAAAG AGCAAAGAAG CAACGGGGAA ACAAAGTGCC ATGTCACCTG 
GTTGTCCTGA CAAACGGCAT GTCCCAGGAC AGCGTCCTGG GTCCCGCGCA TAAGCTGAGG 
GAGGAAAACA TCCGTGTGCA TGCGATCGGC GTCAAGGAAG CCAACCAAAC ACAGCTGCGG 
GAGATAGCGG GAGATGAAAA GCGAGTTTAT TACGTGCATG AATTCGATGC CTTGAGGGAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA TATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATTGGAC TCGAAAACTT CAGCAAGATG 
AAGGTGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGTGTGGTCC AGTTCAGCCA CGAAAACAGG GAGGAGTTTC AGCTCAACAC ATTCATGTCT 
CAAAATGACA TTGCCAACGC CATCGACCAA ATGGCTCACA TTGGAGAGAC AACCTTGACA 
GGCAGTGCGC TGACCTTTGT GTCTCAGTAC TTCAGTCCTG AAAAGGGGGC CCGGCCCAAT 
GTTAGGAAGT TCCTCATTCT CATCACGGAT GGCGAGGCTC AGGACATAGT AAAGGACCCA 
GCAGTAGCCC TTCGAAAAGA CGGTGTGATT ATCTATTCTG TGGGAGTGTT CGGCTCCAAT 
GTCACCCAGC TCGAGGAGAT CAGTGGGAAG CCGGAGATGG TTTTCTATGT TGAGAATTTT 
GACATCCTAC AGCATATTGA AGATGATCTC GTTCTGGGAA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTAGTG TTTGTCATCG ACAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCTCTG AAATATGCTG ATGACCCAGA GGTGCTGTTT 
TACCTGGATG AACTGGGCAC AAAGCTGGAG GTGATTTCAG TGCTCCAGAA TGACCAGCCC 
ATGGGTGGTA ACACTTACAC TGCTGAGGCC CTGGCTTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACTGATGGA 
GAATCTCATG ATGCAGAGAA GCTCAATGCC ACCGCCAAGG CCCTGAGGGA CAAAGGCATT 
CTGGTCCTAG CTGTGGGGAT TGCTGGCGCC AATACCTGGG AGCTCTTGGC CATGGCTGGG 
TCGAGTGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGGAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTCGATTGCG AAATTGAAAA AGTTGACCTC 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGAG 
TTTTTGGCAT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGAGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACATTCAC AGGGGAAAAG 
GAGATATCCA CACAGATTGA GGCCATCCAG CAGATCTTTG GTTACACCCA CATTGGAGAT 
GCACTCAGGA AGGTGAAGCA TTACTTTCGG CCAGACACGG GCAGCAGGAT CAACGCCGGT 
ACCCCTCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGATGAGGT GGCCCAGGCG 
GCTGAGGAGC TGAGACACAA GGGTGTGGAC ATCTACTCGG TGGGCATCGG GGACGTGGAT 
GACCAGCAGC TGATCCAGAT AACCGGGACA GCGGAGAAAA AACTGACGGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGTAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGTT TCGTGGATGT GGTGGTTGGA TTTGATATCT CAAGTCAGCG GAGAGGGCAG 
ACTTTGCTCG AAGGTCAGCC TTGGATGGGG ACCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAGGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ATGCTATGGA GAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTGAGCAGCC TGCAGGGTGT GACAGTGAAC GGCCCATCTC GCCTCAACCC AAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAACAAG TCCGCTGCTC GAGGGAAGGT GGTCCTTCTG 
TTTTCAGATG GATTGGATGA CGGCGTTGAA AAACTTGAAC AGAAATCTGA TGAACTCAGA 
AAGGAAGGCC TGAATGCCCT CATAACCATC GCTGTGGACG GAGCTGCCGA TTCCAGTGAC 
CTGGCTGACC TTCTCTACAT TGAATTTGGG AAAGGATTTG AGTACAGAAC ACAGTTCACA 
ATCGGAATGG GGAACCTTGG GAGTCACCTG TCAAAGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTTTGCTCTG CAAGTGCACA GGATGGGACG GCGCCATGGG GGATCCGGGC 
TCAGCAGGGG AAAAGGGACC CCCAGGATTT AAAGGCAGTG AAGGCTACCT GGGAGAAGAA 
GGCATTGCTG GAGAAAGAGG AGCCTTGGGA CCAATGGGAG AGCAAGGTAC AAAGGGATGC 
TTAGGTGCCA AAGGGCCCAA GGGAGCCCGA GGACTCAGCG GAGAAGAGGG TGAAGTTGGG 
GAAGATGGGC TTGACGGATT AAATGGAGAA CAGGGTGACA GTGGGATTCC TGGAAGAAGA 
GGAGAAAGGG GTGATGCGGG ATCTCAGGGA AGCCCAGGCA AGAGAGGGGC TTCTGGTGAC 
CGTGGACCGA AGGGCCTACG AGGAGATCCG GGAACTCCTG GACGTGATAA TAGCATACAG 
GGACCCAAGG GCTTGAAAGG AGACCCTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGGACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACATACAGGC 
CCACAGGGAA ATCCAGGCAT CACAGGCCCA GATGGACTTG AAGGTTCGCC CGGACTTAAG 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAGG CTTCGGAATG 
AAAGGTCCCC AGGGGCCTCC AGGACCCAAA GGACGAGCTG GGAACCAAGG CCATTGGGGA 
AGCCAAGGAA GCAAAGGAGA ACCTGGAGAC CTGGGGGAAA AGGGAGCTGC TGGCTTTCCA 
GGGCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCAGGAT ATGGTAGCAT TGGCCGAAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC CAAAGGGTGA TATTGGGGAC 
CCTGGCGATC CAGGAGAGGC TGGTCCCAAG GGAGCCAGAG GCAAAACGGT ATCTGCTGGG 
ATTCCAGGAG AGCCAGGATC CCCCGGGGAG CCAGGACCCC CTGGAAGGAA GGGCATGAAA 
GGAGCCCGAG GACTGGCTTC ATTTTCCACA TGTGACCTCA TTCAGTATGT GCGGGAACAC 
AGTCCTGTCA GACACGGAAA ACCTGAGTGC CCAGTGCATC CAACTGAGTT GGTGTTTGTC 
CTGGACCAGT CCCGGGATGT CACAGAACAG GACTTTGAGC GGATGAAGGG GATGATGGCC 
TCCTTAGTTA GGGACATCAA AGTTAGGGAT GCCAGCTGCC CCGTGGGTGC CCGCGTTGCC 
ATCCTAGCCT ACAACTCCCA CACCAGGCAC CTCATCCGCT TCTCGGATGC CTACAGGAAG 
GACCAGCTGC TCAGGGAAAT TAAAGCTCTT CCTTACGAGA AATCCTCCGA CAGCAGGGAG 
ATTGGCAAAG CCATGAGGTT TATCTCCAGG AATGTCTTCA AGAGAACCCT CCCAGGGGCT 
CACGTGAGAA GAATCGCCAC GTTCTTTAGC AGTGGCCAGT CAGCCGATGT CCAGTCCATT 
ACCACAGCTG CCATGGAGTT CAGTGCCCTG GACATCATAC CAGTGGTGAT TGCATTCAGC 
AACGTGCCCT CGGTCAAGCG TGCCTTTTCG ACCTATGCAA GCCAGATGCT TCTTGTGACC 
AAGCCAAACC CCCCATTCAG TCCTACCTGG ATGCTGCATT CCTGCTGGAT AGCTCCTGGC 
ATGTGGGAGG TGCAGAATTT GAAGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a6 ( XM_017603711.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017459200.1 Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X7, mRNA.

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CloneID	ORa55950
Clone ID Related Accession (Same CDS sequence)	XM_017603711.1
Accession Version	XM_017603711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-6(VI) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000076.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGA TTTTGTTCCT GATGATGATT TGTTTACACG TTTGTGCGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGATGT GGTTTTTCTG GTGGACAGTT CCGATCACCT AGGGATGAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAA AAGCTAGTCA GCAGCCTCCC CGTAGAGGCC 
AACAAGTACC GTGTGGCCCT GGCCCAGTAC AGTGATGCTC TCCACAATGA GTTCCACCTA 
GGCGCCTTCA AGAACAGGAA CCCCATGCTG AACCACCTCA AGAAGAACTT CGGGTTCATC 
GGCGGCTCCC TGAAGATTGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAACG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCG 
GAGTCCGAGG ATGACGTAGA AGAGGCTTCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAGACCTGA AGGCCATGGC CACCTCTCAG 
TTTCATTTCA ACCTCAGGAC TGCCAGAGAC CTCAGCATGT TTGCCCCAAA CATGACACAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GCAATGACTG ATGACATTAT TGTAGAAGCT 
TGCCAAGGCC CATCTGTGGC CGATGTGGTA TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAAGAGAACC TAGATCATCT TAAAGCATTC CTGGGGGAAA GTGTCTCTGC CCTTGACATA 
AAGGAAAACT GCATGAGGGT TGGCCTGGTG GCCTATAGCA ACGAAACAAG GGTGATCAGT 
TCTCTGAGCA TGGGTGTCAA CAAGACGGAG GTCTTACAGC GCATACAGGA TCTGTCCCCT 
CACGTAGGGC AGGCCTACAC AGGAGCTGCC CTCAGAAAGA CGAGGAAGGA AGTCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTGCCTCAGA TTGCTGTGCT GGTGACCCAC 
AGAGCTTCAG ATGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
GTCTTTACCA TGGGCGTAGA GGGGGCTAAC CCAGAACAGC TGGAGAAGAT CGCATCCTAC 
CCTGCCGAGC AGTTCACCTC CAAACTGAGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAGCTGAG GAACCAAATC ACACACACAG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCAGC CTGTGTGGAC ACAGAGGAAG CGGATATCTA TCTGCTCATT 
GATGGTTCAG GGAACACGCA GCCCACAGAC TTCCATGAGA TGAAGATCTT CCTGTCCGAG 
GTGGTGGACA TGTTCAACAT CGCTCCCCAC AAGGTGCGAG TCGGGGCCGT GCAGTACGCC 
GACACCTGGG ACCTGGAATT TGAGATCTCT AAGTATACTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGACA ACATCAGGCA GATGGGTGGA AACACCAATA CGGGTGCAGC CCTGAACTTC 
ACCCTGACGC TGTTGCAAAG AGCAAAGAAG CAACGGGGAA ACAAAGTGCC ATGTCACCTG 
GTTGTCCTGA CAAACGGCAT GTCCCAGGAC AGCGTCCTGG GTCCCGCGCA TAAGCTGAGG 
GAGGAAAACA TCCGTGTGCA TGCGATCGGC GTCAAGGAAG CCAACCAAAC ACAGCTGCGG 
GAGATAGCGG GAGATGAAAA GCGAGTTTAT TACGTGCATG AATTCGATGC CTTGAGGGAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA TATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATTGGAC TCGAAAACTT CAGCAAGATG 
AAGGTGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGTGTGGTCC AGTTCAGCCA CGAAAACAGG GAGGAGTTTC AGCTCAACAC ATTCATGTCT 
CAAAATGACA TTGCCAACGC CATCGACCAA ATGGCTCACA TTGGAGAGAC AACCTTGACA 
GGCAGTGCGC TGACCTTTGT GTCTCAGTAC TTCAGTCCTG AAAAGGGGGC CCGGCCCAAT 
GTTAGGAAGT TCCTCATTCT CATCACGGAT GGCGAGGCTC AGGACATAGT AAAGGACCCA 
GCAGTAGCCC TTCGAAAAGA CGGTGTGATT ATCTATTCTG TGGGAGTGTT CGGCTCCAAT 
GTCACCCAGC TCGAGGAGAT CAGTGGGAAG CCGGAGATGG TTTTCTATGT TGAGAATTTT 
GACATCCTAC AGCATATTGA AGATGATCTC GTTCTGGGAA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTAGTG TTTGTCATCG ACAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCTCTG AAATATGCTG ATGACCCAGA GGTGCTGTTT 
TACCTGGATG AACTGGGCAC AAAGCTGGAG GTGATTTCAG TGCTCCAGAA TGACCAGCCC 
ATGGGTGGTA ACACTTACAC TGCTGAGGCC CTGGCTTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACTGATGGA 
GAATCTCATG ATGCAGAGAA GCTCAATGCC ACCGCCAAGG CCCTGAGGGA CAAAGGCATT 
CTGGTCCTAG CTGTGGGGAT TGCTGGCGCC AATACCTGGG AGCTCTTGGC CATGGCTGGG 
TCGAGTGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGGAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTCGATTGCG AAATTGAAAA AGTTGACCTC 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGAG 
TTTTTGGCAT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGAGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACATTCAC AGGGGAAAAG 
GAGATATCCA CACAGATTGA GGCCATCCAG CAGATCTTTG GTTACACCCA CATTGGAGAT 
GCACTCAGGA AGGTGAAGCA TTACTTTCGG CCAGACACGG GCAGCAGGAT CAACGCCGGT 
ACCCCTCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGATGAGGT GGCCCAGGCG 
GCTGAGGAGC TGAGACACAA GGGTGTGGAC ATCTACTCGG TGGGCATCGG GGACGTGGAT 
GACCAGCAGC TGATCCAGAT AACCGGGACA GCGGAGAAAA AACTGACGGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGTAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGTT TCGTGGATGT GGTGGTTGGA TTTGATATCT CAAGTCAGCG GAGAGGGCAG 
ACTTTGCTCG AAGGTCAGCC TTGGATGGGG ACCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAGGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ATGCTATGGA GAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTGAGCAGCC TGCAGGGTGT GACAGTGAAC GGCCCATCTC GCCTCAACCC AAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAACAAG TCCGCTGCTC GAGGGAAGGT GGTCCTTCTG 
TTTTCAGATG GATTGGATGA CGGCGTTGAA AAACTTGAAC AGAAATCTGA TGAACTCAGA 
AAGGAAGGCC TGAATGCCCT CATAACCATC GCTGTGGACG GAGCTGCCGA TTCCAGTGAC 
CTGGCTGACC TTCTCTACAT TGAATTTGGG AAAGGATTTG AGTACAGAAC ACAGTTCACA 
ATCGGAATGG GGAACCTTGG GAGTCACCTG TCAAAGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTTTGCTCTG CAAGTGCACA GGATGGGACG GCGCCATGGG GGATCCGGGC 
TCAGCAGGGG AAAAGGGACC CCCAGGATTT AAAGGCAGTG AAGGCTACCT GGGAGAAGAA 
GGCATTGCTG GAGAAAGAGG AGCCTTGGGA CCAATGGGAG AGCAAGGTAC AAAGGGATGC 
TTAGGTGCCA AAGGGCCCAA GGGAGCCCGA GGACTCAGCG GAGAAGAGGG TGAAGTTGGG 
GAAGATGGGC TTGACGGATT AAATGGAGAA CAGGGTGACA GTGGGATTCC TGGAAGAAGA 
GGAGAAAGGG GTGATGCGGG ATCTCAGGGA AGCCCAGGCA AGAGAGGGGC TTCTGGTGAC 
CGTGGACCGA AGGGCCTACG AGGAGATCCG GGAACTCCTG GACGTGATAA TAGCATACAG 
GGACCCAAGG GCTTGAAAGG AGACCCTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGGACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACATACAGGC 
CCACAGGGAA ATCCAGGCAT CACAGGCCCA GATGGACTTG AAGGTTCGCC CGGACTTAAG 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAGG CTTCGGAATG 
AAAGGTCCCC AGGGGCCTCC AGGACCCAAA GGACGAGCTG GGAACCAAGG CCATTGGGGA 
AGCCAAGGAA GCAAAGGAGA ACCTGGAGAC CTGGGGGAAA AGGGAGCTGC TGGCTTTCCA 
GGGCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCAGGAT ATGGTAGCAT TGGCCGAAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC CAAAGGGTGA TATTGGGGAC 
CCTGGCGATC CAGGAGAGGC TGGTCCCAAG GGAGCCAGAG GCAAAACGAT AGGGCCAGCC 
TCTGGACACC AAGAATTCAG CACATCAGCG GGTGTGAGGG GAGTTTCGTA TGTAAACCCT 
AACAAGCCGA TAAGAGGAAA CAGGCGCCGG GAAGGTTTGA CGATACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017459200.1
CDS	38..5248
Translation	MLLILFLMMICLHVCANQDSGPEYADVVFLVDSSDHLGMKSFPLVKTFIQKLVSSLPVEANKYRVALAQYSDALHNEFHLGAFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEASKALREDGVKIISVGVQKASEEDLKAMATSQFHFNLRTARDLSMFAPNMTQIIKDVTQYREAMTDDIIVEACQGPSVADVVFLLDMAINGSQENLDHLKAFLGESVSALDIKENCMRVGLVAYSNETRVISSLSMGVNKTEVLQRIQDLSPHVGQAYTGAALRKTRKEVFSAQRGSRKNQGVPQIAVLVTHRASDDNVTKAAVNLRREGVTVFTMGVEGANPEQLEKIASYPAEQFTSKLSNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGNTQPTDFHEMKIFLSEVVDMFNIAPHKVRVGAVQYADTWDLEFEISKYTNKPDLGKAIDNIRQMGGNTNTGAALNFTLTLLQRAKKQRGNKVPCHLVVLTNGMSQDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGDEKRVYYVHEFDALRDIRNQVVQEICAEEACRDMKADIMFLVDSSGSIGLENFSKMKVFMKNLVSKSQIGADRVQIGVVQFSHENREEFQLNTFMSQNDIANAIDQMAHIGETTLTGSALTFVSQYFSPEKGARPNVRKFLILITDGEAQDIVKDPAVALRKDGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVISVLQNDQPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNATAKALRDKGILVLAVGIAGANTWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFQKMKEFLASVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEKEISTQIEAIQQIFGYTHIGDALRKVKHYFRPDTGSRINAGTPQVLLVLTDGRSQDEVAQAAEELRHKGVDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVKKRIVRNICTSGGESNCFVDVVVGFDISSQRRGQTLLEGQPWMGTYLQDLLRAISSLNGVSCEVGTETQVSIAFQVTNAMERYPSKFEIYSENILSSLQGVTVNGPSRLNPNLLSSLWDTFQNKSAARGKVVLLFSDGLDDGVEKLEQKSDELRKEGLNALITIAVDGAADSSDLADLLYIEFGKGFEYRTQFTIGMGNLGSHLSKQLINVAERTCCCLLCKCTGWDGAMGDPGSAGEKGPPGFKGSEGYLGEEGIAGERGALGPMGEQGTKGCLGAKGPKGARGLSGEEGEVGEDGLDGLNGEQGDSGIPGRRGERGDAGSQGSPGKRGASGDRGPKGLRGDPGTPGRDNSIQGPKGLKGDPGRQGRRGWPGSPGTPGSRRKMVVHGRRGHTGPQGNPGITGPDGLEGSPGLKGPQGPRGEVGEKGEKGGFGMKGPQGPPGPKGRAGNQGHWGSQGSKGEPGDLGEKGAAGFPGPRGLQGDDGSPGYGSIGRKGTKGQEGFPGESGPKGDIGDPGDPGEAGPKGARGKTIGPASGHQEFSTSAGVRGVSYVNPNKPIRGNRRREGLTIQ

Target ORF information:

RefSeq Version	XM_017603711.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017603711.1

ORF Insert Sequence:

ATGCTGCTGA TTTTGTTCCT GATGATGATT TGTTTACACG TTTGTGCGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGATGT GGTTTTTCTG GTGGACAGTT CCGATCACCT AGGGATGAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAA AAGCTAGTCA GCAGCCTCCC CGTAGAGGCC 
AACAAGTACC GTGTGGCCCT GGCCCAGTAC AGTGATGCTC TCCACAATGA GTTCCACCTA 
GGCGCCTTCA AGAACAGGAA CCCCATGCTG AACCACCTCA AGAAGAACTT CGGGTTCATC 
GGCGGCTCCC TGAAGATTGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAACG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCG 
GAGTCCGAGG ATGACGTAGA AGAGGCTTCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAGACCTGA AGGCCATGGC CACCTCTCAG 
TTTCATTTCA ACCTCAGGAC TGCCAGAGAC CTCAGCATGT TTGCCCCAAA CATGACACAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GCAATGACTG ATGACATTAT TGTAGAAGCT 
TGCCAAGGCC CATCTGTGGC CGATGTGGTA TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAAGAGAACC TAGATCATCT TAAAGCATTC CTGGGGGAAA GTGTCTCTGC CCTTGACATA 
AAGGAAAACT GCATGAGGGT TGGCCTGGTG GCCTATAGCA ACGAAACAAG GGTGATCAGT 
TCTCTGAGCA TGGGTGTCAA CAAGACGGAG GTCTTACAGC GCATACAGGA TCTGTCCCCT 
CACGTAGGGC AGGCCTACAC AGGAGCTGCC CTCAGAAAGA CGAGGAAGGA AGTCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTGCCTCAGA TTGCTGTGCT GGTGACCCAC 
AGAGCTTCAG ATGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
GTCTTTACCA TGGGCGTAGA GGGGGCTAAC CCAGAACAGC TGGAGAAGAT CGCATCCTAC 
CCTGCCGAGC AGTTCACCTC CAAACTGAGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAGCTGAG GAACCAAATC ACACACACAG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCAGC CTGTGTGGAC ACAGAGGAAG CGGATATCTA TCTGCTCATT 
GATGGTTCAG GGAACACGCA GCCCACAGAC TTCCATGAGA TGAAGATCTT CCTGTCCGAG 
GTGGTGGACA TGTTCAACAT CGCTCCCCAC AAGGTGCGAG TCGGGGCCGT GCAGTACGCC 
GACACCTGGG ACCTGGAATT TGAGATCTCT AAGTATACTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGACA ACATCAGGCA GATGGGTGGA AACACCAATA CGGGTGCAGC CCTGAACTTC 
ACCCTGACGC TGTTGCAAAG AGCAAAGAAG CAACGGGGAA ACAAAGTGCC ATGTCACCTG 
GTTGTCCTGA CAAACGGCAT GTCCCAGGAC AGCGTCCTGG GTCCCGCGCA TAAGCTGAGG 
GAGGAAAACA TCCGTGTGCA TGCGATCGGC GTCAAGGAAG CCAACCAAAC ACAGCTGCGG 
GAGATAGCGG GAGATGAAAA GCGAGTTTAT TACGTGCATG AATTCGATGC CTTGAGGGAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA TATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATTGGAC TCGAAAACTT CAGCAAGATG 
AAGGTGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGTGTGGTCC AGTTCAGCCA CGAAAACAGG GAGGAGTTTC AGCTCAACAC ATTCATGTCT 
CAAAATGACA TTGCCAACGC CATCGACCAA ATGGCTCACA TTGGAGAGAC AACCTTGACA 
GGCAGTGCGC TGACCTTTGT GTCTCAGTAC TTCAGTCCTG AAAAGGGGGC CCGGCCCAAT 
GTTAGGAAGT TCCTCATTCT CATCACGGAT GGCGAGGCTC AGGACATAGT AAAGGACCCA 
GCAGTAGCCC TTCGAAAAGA CGGTGTGATT ATCTATTCTG TGGGAGTGTT CGGCTCCAAT 
GTCACCCAGC TCGAGGAGAT CAGTGGGAAG CCGGAGATGG TTTTCTATGT TGAGAATTTT 
GACATCCTAC AGCATATTGA AGATGATCTC GTTCTGGGAA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTAGTG TTTGTCATCG ACAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCTCTG AAATATGCTG ATGACCCAGA GGTGCTGTTT 
TACCTGGATG AACTGGGCAC AAAGCTGGAG GTGATTTCAG TGCTCCAGAA TGACCAGCCC 
ATGGGTGGTA ACACTTACAC TGCTGAGGCC CTGGCTTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACTGATGGA 
GAATCTCATG ATGCAGAGAA GCTCAATGCC ACCGCCAAGG CCCTGAGGGA CAAAGGCATT 
CTGGTCCTAG CTGTGGGGAT TGCTGGCGCC AATACCTGGG AGCTCTTGGC CATGGCTGGG 
TCGAGTGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGGAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTCGATTGCG AAATTGAAAA AGTTGACCTC 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGAG 
TTTTTGGCAT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGAGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACATTCAC AGGGGAAAAG 
GAGATATCCA CACAGATTGA GGCCATCCAG CAGATCTTTG GTTACACCCA CATTGGAGAT 
GCACTCAGGA AGGTGAAGCA TTACTTTCGG CCAGACACGG GCAGCAGGAT CAACGCCGGT 
ACCCCTCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGATGAGGT GGCCCAGGCG 
GCTGAGGAGC TGAGACACAA GGGTGTGGAC ATCTACTCGG TGGGCATCGG GGACGTGGAT 
GACCAGCAGC TGATCCAGAT AACCGGGACA GCGGAGAAAA AACTGACGGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGTAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGTT TCGTGGATGT GGTGGTTGGA TTTGATATCT CAAGTCAGCG GAGAGGGCAG 
ACTTTGCTCG AAGGTCAGCC TTGGATGGGG ACCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAGGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ATGCTATGGA GAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTGAGCAGCC TGCAGGGTGT GACAGTGAAC GGCCCATCTC GCCTCAACCC AAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAACAAG TCCGCTGCTC GAGGGAAGGT GGTCCTTCTG 
TTTTCAGATG GATTGGATGA CGGCGTTGAA AAACTTGAAC AGAAATCTGA TGAACTCAGA 
AAGGAAGGCC TGAATGCCCT CATAACCATC GCTGTGGACG GAGCTGCCGA TTCCAGTGAC 
CTGGCTGACC TTCTCTACAT TGAATTTGGG AAAGGATTTG AGTACAGAAC ACAGTTCACA 
ATCGGAATGG GGAACCTTGG GAGTCACCTG TCAAAGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTTTGCTCTG CAAGTGCACA GGATGGGACG GCGCCATGGG GGATCCGGGC 
TCAGCAGGGG AAAAGGGACC CCCAGGATTT AAAGGCAGTG AAGGCTACCT GGGAGAAGAA 
GGCATTGCTG GAGAAAGAGG AGCCTTGGGA CCAATGGGAG AGCAAGGTAC AAAGGGATGC 
TTAGGTGCCA AAGGGCCCAA GGGAGCCCGA GGACTCAGCG GAGAAGAGGG TGAAGTTGGG 
GAAGATGGGC TTGACGGATT AAATGGAGAA CAGGGTGACA GTGGGATTCC TGGAAGAAGA 
GGAGAAAGGG GTGATGCGGG ATCTCAGGGA AGCCCAGGCA AGAGAGGGGC TTCTGGTGAC 
CGTGGACCGA AGGGCCTACG AGGAGATCCG GGAACTCCTG GACGTGATAA TAGCATACAG 
GGACCCAAGG GCTTGAAAGG AGACCCTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGGACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACATACAGGC 
CCACAGGGAA ATCCAGGCAT CACAGGCCCA GATGGACTTG AAGGTTCGCC CGGACTTAAG 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAGG CTTCGGAATG 
AAAGGTCCCC AGGGGCCTCC AGGACCCAAA GGACGAGCTG GGAACCAAGG CCATTGGGGA 
AGCCAAGGAA GCAAAGGAGA ACCTGGAGAC CTGGGGGAAA AGGGAGCTGC TGGCTTTCCA 
GGGCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCAGGAT ATGGTAGCAT TGGCCGAAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC CAAAGGGTGA TATTGGGGAC 
CCTGGCGATC CAGGAGAGGC TGGTCCCAAG GGAGCCAGAG GCAAAACGAT AGGGCCAGCC 
TCTGGACACC AAGAATTCAG CACATCAGCG GGTGTGAGGG GAGTTTCGTA TGTAAACCCT 
AACAAGCCGA TAAGAGGAAA CAGGCGCCGG GAAGGTTTGA CGATACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a6 ( XM_017596190.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451679.1 Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X1, mRNA.

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CloneID	ORa62615
Clone ID Related Accession (Same CDS sequence)	XM_017596190.1
Accession Version	XM_017596190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-6(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCTGCTGA TTTTGTTCCT GATGATGATT TGTTTACACG TTTGTGCGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGATGT GGTTTTTCTG GTGGACAGTT CCGATCACCT AGGGATGAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAA AAGCTAGTCA GCAGCCTCCC CGTAGAGGCC 
AACAAGTACC GTGTGGCCCT GGCCCAGTAC AGTGATGCTC TCCACAATGA GTTCCACCTA 
GGCGCCTTCA AGAACAGGAA CCCCATGCTG AACCACCTCA AGAAGAACTT CGGGTTCATC 
GGCGGCTCCC TGAAGATTGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAACG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCG 
GAGTCCGAGG ATGACGTAGA AGAGGCTTCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAGACCTGA AGGCCATGGC CACCTCTCAG 
TTTCATTTCA ACCTCAGGAC TGCCAGAGAC CTCAGCATGT TTGCCCCAAA CATGACACAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GCAATGACTG ATGACATTAT TGTAGAAGCT 
TGCCAAGGCC CATCTGTGGC CGATGTGGTA TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAAGAGAACC TAGATCATCT TAAAGCATTC CTGGGGGAAA GTGTCTCTGC CCTTGACATA 
AAGGAAAACT GCATGAGGGT TGGCCTGGTG GCCTATAGCA ACGAAACAAG GGTGATCAGT 
TCTCTGAGCA TGGGTGTCAA CAAGACGGAG GTCTTACAGC GCATACAGGA TCTGTCCCCT 
CACGTAGGGC AGGCCTACAC AGGAGCTGCC CTCAGAAAGA CGAGGAAGGA AGTCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTGCCTCAGA TTGCTGTGCT GGTGACCCAC 
AGAGCTTCAG ATGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
GTCTTTACCA TGGGCGTAGA GGGGGCTAAC CCAGAACAGC TGGAGAAGAT CGCATCCTAC 
CCTGCCGAGC AGTTCACCTC CAAACTGAGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAGCTGAG GAACCAAATC ACACACACAG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCAGC CTGTGTGGAC ACAGAGGAAG CGGATATCTA TCTGCTCATT 
GATGGTTCAG GGAACACGCA GCCCACAGAC TTCCATGAGA TGAAGATCTT CCTGTCCGAG 
GTGGTGGACA TGTTCAACAT CGCTCCCCAC AAGGTGCGAG TCGGGGCCGT GCAGTACGCC 
GACACCTGGG ACCTGGAATT TGAGATCTCT AAGTATACTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGACA ACATCAGGCA GATGGGTGGA AACACCAATA CGGGTGCAGC CCTGAACTTC 
ACCCTGACGC TGTTGCAAAG AGCAAAGAAG CAACGGGGAA ACAAAGTGCC ATGTCACCTG 
GTTGTCCTGA CAAACGGCAT GTCCCAGGAC AGCGTCCTGG GTCCCGCGCA TAAGCTGAGG 
GAGGAAAACA TCCGTGTGCA TGCGATCGGC GTCAAGGAAG CCAACCAAAC ACAGCTGCGG 
GAGATAGCGG GAGATGAAAA GCGAGTTTAT TACGTGCATG AATTCGATGC CTTGAGGGAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA TATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATTGGAC TCGAAAACTT CAGCAAGATG 
AAGGTGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGTGTGGTCC AGTTCAGCCA CGAAAACAGG GAGGAGTTTC AGCTCAACAC ATTCATGTCT 
CAAAATGACA TTGCCAACGC CATCGACCAA ATGGCTCACA TTGGAGAGAC AACCTTGACA 
GGCAGTGCGC TGACCTTTGT GTCTCAGTAC TTCAGTCCTG AAAAGGGGGC CCGGCCCAAT 
GTTAGGAAGT TCCTCATTCT CATCACGGAT GGCGAGGCTC AGGACATAGT AAAGGACCCA 
GCAGTAGCCC TTCGAAAAGA CGGTGTGATT ATCTATTCTG TGGGAGTGTT CGGCTCCAAT 
GTCACCCAGC TCGAGGAGAT CAGTGGGAAG CCGGAGATGG TTTTCTATGT TGAGAATTTT 
GACATCCTAC AGCATATTGA AGATGATCTC GTTCTGGGAA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTAGTG TTTGTCATCG ACAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCTCTG AAATATGCTG ATGACCCAGA GGTGCTGTTT 
TACCTGGATG AACTGGGCAC AAAGCTGGAG GTGATTTCAG TGCTCCAGAA TGACCAGCCC 
ATGGGTGGTA ACACTTACAC TGCTGAGGCC CTGGCTTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACTGATGGA 
GAATCTCATG ATGCAGAGAA GCTCAATGCC ACCGCCAAGG CCCTGAGGGA CAAAGGCATT 
CTGGTCCTAG CTGTGGGGAT TGCTGGCGCC AATACCTGGG AGCTCTTGGC CATGGCTGGG 
TCGAGTGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGGAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTCGATTGCG AAATTGAAAA AGTTGACCTC 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGAG 
TTTTTGGCAT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGAGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACATTCAC AGGGGAAAAG 
GAGATATCCA CACAGATTGA GGCCATCCAG CAGATCTTTG GTTACACCCA CATTGGAGAT 
GCACTCAGGA AGGTGAAGCA TTACTTTCGG CCAGACACGG GCAGCAGGAT CAACGCCGGT 
ACCCCTCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGATGAGGT GGCCCAGGCG 
GCTGAGGAGC TGAGACACAA GGGTGTGGAC ATCTACTCGG TGGGCATCGG GGACGTGGAT 
GACCAGCAGC TGATCCAGAT AACCGGGACA GCGGAGAAAA AACTGACGGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGTAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGTT TCGTGGATGT GGTGGTTGGA TTTGATATCT CAAGTCAGCG GAGAGGGCAG 
ACTTTGCTCG AAGGTCAGCC TTGGATGGGG ACCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAGGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ATGCTATGGA GAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTGAGCAGCC TGCAGGGTGT GACAGTGAAC GGCCCATCTC GCCTCAACCC AAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAACAAG TCCGCTGCTC GAGGGAAGGT GGTCCTTCTG 
TTTTCAGATG GATTGGATGA CGGCGTTGAA AAACTTGAAC AGAAATCTGA TGAACTCAGA 
AAGGAAGGCC TGAATGCCCT CATAACCATC GCTGTGGACG GAGCTGCCGA TTCCAGTGAC 
CTGGCTGACC TTCTCTACAT TGAATTTGGG AAAGGATTTG AGTACAGAAC ACAGTTCACA 
ATCGGAATGG GGAACCTTGG GAGTCACCTG TCAAAGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTTTGCTCTG CAAGTGCACA GGATGGGACG GCGCCATGGG GGATCCGGGC 
TCAGCAGGGG AAAAGGGACC CCCAGGATTT AAAGGCAGTG AAGGCTACCT GGGAGAAGAA 
GGCATTGCTG GAGAAAGAGG AGCCTTGGGA CCAATGGGAG AGCAAGGTAC AAAGGGATGC 
TTAGGTGCCA AAGGGCCCAA GGGAGCCCGA GGACTCAGCG GAGAAGAGGG TGAAGTTGGG 
GAAGATGGGC TTGACGGATT AAATGGAGAA CAGGGTGACA GTGGGATTCC TGGAAGAAGA 
GGAGAAAGGG GTGATGCGGG ATCTCAGGGA AGCCCAGGCA AGAGAGGGGC TTCTGGTGAC 
CGTGGACCGA AGGGCCTACG AGGAGATCCG GGAACTCCTG GACGTGATAA TAGCATACAG 
GGACCCAAGG GCTTGAAAGG AGACCCTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGGACAC CAGGCTCAAG AAGAAAGATG GTAAGAGCCC TTGGAGGCAA GGAGCTTTCA 
GACTCATGGA TCCCATCGCT AGGTGACAAG AGGGATCAGA TTGGTTCCCC TGTGTCTACA 
GATGGTTATG AGGTTGGTTC TCTCTCATCA CAGGGAAATC CAGGCATCAC AGGCCCAGAT 
GGACTTGAAG GTTCGCCCGG ACTTAAGGGC CCGCAGGGTC CAAGAGGAGA GGTCGGTGAG 
AAAGGAGAAA AAGGAGGCTT CGGAATGAAA GGTCCCCAGG GGCCTCCAGG ACCCAAAGGA 
CGAGCTGGGA ACCAAGGCCA TTGGGGAAGC CAAGGAAGCA AAGGAGAACC TGGAGACCTG 
GGGGAAAAGG GAGCTGCTGG CTTTCCAGGG CCTCGGGGCC TGCAGGGTGA CGACGGCAGC 
CCAGGATATG GTAGCATTGG CCGAAAAGGG ACAAAGGGGC AAGAAGGATT CCCCGGAGAG 
AGTGGACCAA AGGGTGATAT TGGGGACCCT GGCGATCCAG GAGAGGCTGG TCCCAAGGGA 
GCCAGAGGCA AAACGGTATC TGCTGGGATT CCAGGAGAGC CAGGATCCCC CGGGGAGCCA 
GGACCCCCTG GAAGGAAGGG CATGAAAGGA GCCCGAGGAC TGGCTTCATT TTCCACATGT 
GACCTCATTC AGTATGTGCG GGAACACAGT CCTGTCAGAC ACGGAAAACC TGAGTGCCCA 
GTGCATCCAA CTGAGTTGGT GTTTGTCCTG GACCAGTCCC GGGATGTCAC AGAACAGGAC 
TTTGAGCGGA TGAAGGGGAT GATGGCCTCC TTAGTTAGGG ACATCAAAGT TAGGGATGCC 
AGCTGCCCCG TGGGTGCCCG CGTTGCCATC CTAGCCTACA ACTCCCACAC CAGGCACCTC 
ATCCGCTTCT CGGATGCCTA CAGGAAGGAC CAGCTGCTCA GGGAAATTAA AGCTCTTCCT 
TACGAGAAAT CCTCCGACAG CAGGGAGATT GGCAAAGCCA TGAGGTTTAT CTCCAGGAAT 
GTCTTCAAGA GAACCCTCCC AGGGGCTCAC GTGAGAAGAA TCGCCACGTT CTTTAGCAGT 
GGCCAGTCAG CCGATGTCCA GTCCATTACC ACAGCTGCCA TGGAGTTCAG TGCCCTGGAC 
ATCATACCAG TGGTGATTGC ATTCAGCAAC GTGCCCTCGG TCAAGCGTGC CTTTTCGATT 
GATGACACTG GTACATTCCA AGTCCTTGTG GTTCCCTCCA GGCCTGACCA AGTGCCAGCG 
TTAGAGAGAC TCCAGCGGTG CACTTTCTGC TATGACCTAT GCAAGCCAGA TGCTTCTTGT 
GACCAAGCCA AACCCCCCAT TCAGTCCTAC CTGGATGCTG CATTCCTGCT GGATAGCTCC 
TGGCATGTGG GAGGTGCAGA ATTTGAAGAC ATGAGAGACC TCCTGGAGGC CCTGCTAGAT 
CACTTTGAAA TCGCTTCAGA GCCAGAGACG TCTGTCACTG GAGACAGGGT GGCCCTACTG 
AGTCATGCTC CCGTTGACTT CCTACCCAAC ACCCAGAGGA GTCCCGTTAG AACTGAGTTC 
AACCTAACCA CCTATAGCAG TAAGCACCTC ATGAAGAGAC ACGTGGAGCA AGCAGTCCAG 
CAGCTGAATG GAGATGCTTT CCTTGGTCAT GCCCTCAGGT GGGCACTGGA CAATGTCTTT 
CTAAACACGC CCAATCTGAG AAGGAACAAA GTCATATTTG TGATATCTGC TGGGGAAACC 
AGCCACCTGG ATGCGGAAAC CTTAAAGAAA GAGTCCCTGC GAGCCAAATG TCATGGGTAT 
GCCCTCTTTG TGTTTTCCTT GGGCCCTGAC TGGGATGACA AAGAACTGGA AGACCTGGCC 
AGCCACCCAC TTGACCAGCA CTTGATCCAG CTTGGCCGAA TTCATAAACC TGACCACGGA 
TATGGTGTAA AGTTTGTGAA GTCCTTTATA AACTCCATCA GGCATGGGAT AAACAAGTAT 
CCACCGGTCA ACTTTAAAGC CAAGTGCAGC AGACTCAGCT CGGCGGCTCC GAAGCTGTCC 
CCACGCCAGT TTCGAAGCTT TGTTCCGGGG CCACAGAAAG CTCTCCTCAA AGACCACACA 
CTGGAGGCAG CAAAGCTCTT TCAAGATAAA AAACGCCTTT CAAGTATCCT GAGAAGTGGC 
AGGGCTGCTC TTTCAAGTCA TTCCAGAAGC ACACGCTACT CCTTTAAACA GGGGAGAGAA 
GCGACAAAAG CTACTTCTAA ACTCGGTAAA CGAAGTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451679.1
CDS	38..6919
Translation	MLLILFLMMICLHVCANQDSGPEYADVVFLVDSSDHLGMKSFPLVKTFIQKLVSSLPVEANKYRVALAQYSDALHNEFHLGAFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEASKALREDGVKIISVGVQKASEEDLKAMATSQFHFNLRTARDLSMFAPNMTQIIKDVTQYREAMTDDIIVEACQGPSVADVVFLLDMAINGSQENLDHLKAFLGESVSALDIKENCMRVGLVAYSNETRVISSLSMGVNKTEVLQRIQDLSPHVGQAYTGAALRKTRKEVFSAQRGSRKNQGVPQIAVLVTHRASDDNVTKAAVNLRREGVTVFTMGVEGANPEQLEKIASYPAEQFTSKLSNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGNTQPTDFHEMKIFLSEVVDMFNIAPHKVRVGAVQYADTWDLEFEISKYTNKPDLGKAIDNIRQMGGNTNTGAALNFTLTLLQRAKKQRGNKVPCHLVVLTNGMSQDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGDEKRVYYVHEFDALRDIRNQVVQEICAEEACRDMKADIMFLVDSSGSIGLENFSKMKVFMKNLVSKSQIGADRVQIGVVQFSHENREEFQLNTFMSQNDIANAIDQMAHIGETTLTGSALTFVSQYFSPEKGARPNVRKFLILITDGEAQDIVKDPAVALRKDGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVISVLQNDQPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNATAKALRDKGILVLAVGIAGANTWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFQKMKEFLASVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEKEISTQIEAIQQIFGYTHIGDALRKVKHYFRPDTGSRINAGTPQVLLVLTDGRSQDEVAQAAEELRHKGVDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVKKRIVRNICTSGGESNCFVDVVVGFDISSQRRGQTLLEGQPWMGTYLQDLLRAISSLNGVSCEVGTETQVSIAFQVTNAMERYPSKFEIYSENILSSLQGVTVNGPSRLNPNLLSSLWDTFQNKSAARGKVVLLFSDGLDDGVEKLEQKSDELRKEGLNALITIAVDGAADSSDLADLLYIEFGKGFEYRTQFTIGMGNLGSHLSKQLINVAERTCCCLLCKCTGWDGAMGDPGSAGEKGPPGFKGSEGYLGEEGIAGERGALGPMGEQGTKGCLGAKGPKGARGLSGEEGEVGEDGLDGLNGEQGDSGIPGRRGERGDAGSQGSPGKRGASGDRGPKGLRGDPGTPGRDNSIQGPKGLKGDPGRQGRRGWPGSPGTPGSRRKMVRALGGKELSDSWIPSLGDKRDQIGSPVSTDGYEVGSLSSQGNPGITGPDGLEGSPGLKGPQGPRGEVGEKGEKGGFGMKGPQGPPGPKGRAGNQGHWGSQGSKGEPGDLGEKGAAGFPGPRGLQGDDGSPGYGSIGRKGTKGQEGFPGESGPKGDIGDPGDPGEAGPKGARGKTVSAGIPGEPGSPGEPGPPGRKGMKGARGLASFSTCDLIQYVREHSPVRHGKPECPVHPTELVFVLDQSRDVTEQDFERMKGMMASLVRDIKVRDASCPVGARVAILAYNSHTRHLIRFSDAYRKDQLLREIKALPYEKSSDSREIGKAMRFISRNVFKRTLPGAHVRRIATFFSSGQSADVQSITTAAMEFSALDIIPVVIAFSNVPSVKRAFSIDDTGTFQVLVVPSRPDQVPALERLQRCTFCYDLCKPDASCDQAKPPIQSYLDAAFLLDSSWHVGGAEFEDMRDLLEALLDHFEIASEPETSVTGDRVALLSHAPVDFLPNTQRSPVRTEFNLTTYSSKHLMKRHVEQAVQQLNGDAFLGHALRWALDNVFLNTPNLRRNKVIFVISAGETSHLDAETLKKESLRAKCHGYALFVFSLGPDWDDKELEDLASHPLDQHLIQLGRIHKPDHGYGVKFVKSFINSIRHGINKYPPVNFKAKCSRLSSAAPKLSPRQFRSFVPGPQKALLKDHTLEAAKLFQDKKRLSSILRSGRAALSSHSRSTRYSFKQGREATKATSKLGKRSD

Target ORF information:

RefSeq Version	XM_017596190.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596190.1

ORF Insert Sequence:

ATGCTGCTGA TTTTGTTCCT GATGATGATT TGTTTACACG TTTGTGCGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGATGT GGTTTTTCTG GTGGACAGTT CCGATCACCT AGGGATGAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAA AAGCTAGTCA GCAGCCTCCC CGTAGAGGCC 
AACAAGTACC GTGTGGCCCT GGCCCAGTAC AGTGATGCTC TCCACAATGA GTTCCACCTA 
GGCGCCTTCA AGAACAGGAA CCCCATGCTG AACCACCTCA AGAAGAACTT CGGGTTCATC 
GGCGGCTCCC TGAAGATTGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAACG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCG 
GAGTCCGAGG ATGACGTAGA AGAGGCTTCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAGACCTGA AGGCCATGGC CACCTCTCAG 
TTTCATTTCA ACCTCAGGAC TGCCAGAGAC CTCAGCATGT TTGCCCCAAA CATGACACAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GCAATGACTG ATGACATTAT TGTAGAAGCT 
TGCCAAGGCC CATCTGTGGC CGATGTGGTA TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAAGAGAACC TAGATCATCT TAAAGCATTC CTGGGGGAAA GTGTCTCTGC CCTTGACATA 
AAGGAAAACT GCATGAGGGT TGGCCTGGTG GCCTATAGCA ACGAAACAAG GGTGATCAGT 
TCTCTGAGCA TGGGTGTCAA CAAGACGGAG GTCTTACAGC GCATACAGGA TCTGTCCCCT 
CACGTAGGGC AGGCCTACAC AGGAGCTGCC CTCAGAAAGA CGAGGAAGGA AGTCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTGCCTCAGA TTGCTGTGCT GGTGACCCAC 
AGAGCTTCAG ATGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
GTCTTTACCA TGGGCGTAGA GGGGGCTAAC CCAGAACAGC TGGAGAAGAT CGCATCCTAC 
CCTGCCGAGC AGTTCACCTC CAAACTGAGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAGCTGAG GAACCAAATC ACACACACAG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCAGC CTGTGTGGAC ACAGAGGAAG CGGATATCTA TCTGCTCATT 
GATGGTTCAG GGAACACGCA GCCCACAGAC TTCCATGAGA TGAAGATCTT CCTGTCCGAG 
GTGGTGGACA TGTTCAACAT CGCTCCCCAC AAGGTGCGAG TCGGGGCCGT GCAGTACGCC 
GACACCTGGG ACCTGGAATT TGAGATCTCT AAGTATACTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGACA ACATCAGGCA GATGGGTGGA AACACCAATA CGGGTGCAGC CCTGAACTTC 
ACCCTGACGC TGTTGCAAAG AGCAAAGAAG CAACGGGGAA ACAAAGTGCC ATGTCACCTG 
GTTGTCCTGA CAAACGGCAT GTCCCAGGAC AGCGTCCTGG GTCCCGCGCA TAAGCTGAGG 
GAGGAAAACA TCCGTGTGCA TGCGATCGGC GTCAAGGAAG CCAACCAAAC ACAGCTGCGG 
GAGATAGCGG GAGATGAAAA GCGAGTTTAT TACGTGCATG AATTCGATGC CTTGAGGGAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA TATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATTGGAC TCGAAAACTT CAGCAAGATG 
AAGGTGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGTGTGGTCC AGTTCAGCCA CGAAAACAGG GAGGAGTTTC AGCTCAACAC ATTCATGTCT 
CAAAATGACA TTGCCAACGC CATCGACCAA ATGGCTCACA TTGGAGAGAC AACCTTGACA 
GGCAGTGCGC TGACCTTTGT GTCTCAGTAC TTCAGTCCTG AAAAGGGGGC CCGGCCCAAT 
GTTAGGAAGT TCCTCATTCT CATCACGGAT GGCGAGGCTC AGGACATAGT AAAGGACCCA 
GCAGTAGCCC TTCGAAAAGA CGGTGTGATT ATCTATTCTG TGGGAGTGTT CGGCTCCAAT 
GTCACCCAGC TCGAGGAGAT CAGTGGGAAG CCGGAGATGG TTTTCTATGT TGAGAATTTT 
GACATCCTAC AGCATATTGA AGATGATCTC GTTCTGGGAA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTAGTG TTTGTCATCG ACAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCTCTG AAATATGCTG ATGACCCAGA GGTGCTGTTT 
TACCTGGATG AACTGGGCAC AAAGCTGGAG GTGATTTCAG TGCTCCAGAA TGACCAGCCC 
ATGGGTGGTA ACACTTACAC TGCTGAGGCC CTGGCTTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACTGATGGA 
GAATCTCATG ATGCAGAGAA GCTCAATGCC ACCGCCAAGG CCCTGAGGGA CAAAGGCATT 
CTGGTCCTAG CTGTGGGGAT TGCTGGCGCC AATACCTGGG AGCTCTTGGC CATGGCTGGG 
TCGAGTGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGGAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTCGATTGCG AAATTGAAAA AGTTGACCTC 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGAG 
TTTTTGGCAT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGAGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACATTCAC AGGGGAAAAG 
GAGATATCCA CACAGATTGA GGCCATCCAG CAGATCTTTG GTTACACCCA CATTGGAGAT 
GCACTCAGGA AGGTGAAGCA TTACTTTCGG CCAGACACGG GCAGCAGGAT CAACGCCGGT 
ACCCCTCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGATGAGGT GGCCCAGGCG 
GCTGAGGAGC TGAGACACAA GGGTGTGGAC ATCTACTCGG TGGGCATCGG GGACGTGGAT 
GACCAGCAGC TGATCCAGAT AACCGGGACA GCGGAGAAAA AACTGACGGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGTAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGTT TCGTGGATGT GGTGGTTGGA TTTGATATCT CAAGTCAGCG GAGAGGGCAG 
ACTTTGCTCG AAGGTCAGCC TTGGATGGGG ACCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAGGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ATGCTATGGA GAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTGAGCAGCC TGCAGGGTGT GACAGTGAAC GGCCCATCTC GCCTCAACCC AAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAACAAG TCCGCTGCTC GAGGGAAGGT GGTCCTTCTG 
TTTTCAGATG GATTGGATGA CGGCGTTGAA AAACTTGAAC AGAAATCTGA TGAACTCAGA 
AAGGAAGGCC TGAATGCCCT CATAACCATC GCTGTGGACG GAGCTGCCGA TTCCAGTGAC 
CTGGCTGACC TTCTCTACAT TGAATTTGGG AAAGGATTTG AGTACAGAAC ACAGTTCACA 
ATCGGAATGG GGAACCTTGG GAGTCACCTG TCAAAGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTTTGCTCTG CAAGTGCACA GGATGGGACG GCGCCATGGG GGATCCGGGC 
TCAGCAGGGG AAAAGGGACC CCCAGGATTT AAAGGCAGTG AAGGCTACCT GGGAGAAGAA 
GGCATTGCTG GAGAAAGAGG AGCCTTGGGA CCAATGGGAG AGCAAGGTAC AAAGGGATGC 
TTAGGTGCCA AAGGGCCCAA GGGAGCCCGA GGACTCAGCG GAGAAGAGGG TGAAGTTGGG 
GAAGATGGGC TTGACGGATT AAATGGAGAA CAGGGTGACA GTGGGATTCC TGGAAGAAGA 
GGAGAAAGGG GTGATGCGGG ATCTCAGGGA AGCCCAGGCA AGAGAGGGGC TTCTGGTGAC 
CGTGGACCGA AGGGCCTACG AGGAGATCCG GGAACTCCTG GACGTGATAA TAGCATACAG 
GGACCCAAGG GCTTGAAAGG AGACCCTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGGACAC CAGGCTCAAG AAGAAAGATG GTAAGAGCCC TTGGAGGCAA GGAGCTTTCA 
GACTCATGGA TCCCATCGCT AGGTGACAAG AGGGATCAGA TTGGTTCCCC TGTGTCTACA 
GATGGTTATG AGGTTGGTTC TCTCTCATCA CAGGGAAATC CAGGCATCAC AGGCCCAGAT 
GGACTTGAAG GTTCGCCCGG ACTTAAGGGC CCGCAGGGTC CAAGAGGAGA GGTCGGTGAG 
AAAGGAGAAA AAGGAGGCTT CGGAATGAAA GGTCCCCAGG GGCCTCCAGG ACCCAAAGGA 
CGAGCTGGGA ACCAAGGCCA TTGGGGAAGC CAAGGAAGCA AAGGAGAACC TGGAGACCTG 
GGGGAAAAGG GAGCTGCTGG CTTTCCAGGG CCTCGGGGCC TGCAGGGTGA CGACGGCAGC 
CCAGGATATG GTAGCATTGG CCGAAAAGGG ACAAAGGGGC AAGAAGGATT CCCCGGAGAG 
AGTGGACCAA AGGGTGATAT TGGGGACCCT GGCGATCCAG GAGAGGCTGG TCCCAAGGGA 
GCCAGAGGCA AAACGGTATC TGCTGGGATT CCAGGAGAGC CAGGATCCCC CGGGGAGCCA 
GGACCCCCTG GAAGGAAGGG CATGAAAGGA GCCCGAGGAC TGGCTTCATT TTCCACATGT 
GACCTCATTC AGTATGTGCG GGAACACAGT CCTGTCAGAC ACGGAAAACC TGAGTGCCCA 
GTGCATCCAA CTGAGTTGGT GTTTGTCCTG GACCAGTCCC GGGATGTCAC AGAACAGGAC 
TTTGAGCGGA TGAAGGGGAT GATGGCCTCC TTAGTTAGGG ACATCAAAGT TAGGGATGCC 
AGCTGCCCCG TGGGTGCCCG CGTTGCCATC CTAGCCTACA ACTCCCACAC CAGGCACCTC 
ATCCGCTTCT CGGATGCCTA CAGGAAGGAC CAGCTGCTCA GGGAAATTAA AGCTCTTCCT 
TACGAGAAAT CCTCCGACAG CAGGGAGATT GGCAAAGCCA TGAGGTTTAT CTCCAGGAAT 
GTCTTCAAGA GAACCCTCCC AGGGGCTCAC GTGAGAAGAA TCGCCACGTT CTTTAGCAGT 
GGCCAGTCAG CCGATGTCCA GTCCATTACC ACAGCTGCCA TGGAGTTCAG TGCCCTGGAC 
ATCATACCAG TGGTGATTGC ATTCAGCAAC GTGCCCTCGG TCAAGCGTGC CTTTTCGATT 
GATGACACTG GTACATTCCA AGTCCTTGTG GTTCCCTCCA GGCCTGACCA AGTGCCAGCG 
TTAGAGAGAC TCCAGCGGTG CACTTTCTGC TATGACCTAT GCAAGCCAGA TGCTTCTTGT 
GACCAAGCCA AACCCCCCAT TCAGTCCTAC CTGGATGCTG CATTCCTGCT GGATAGCTCC 
TGGCATGTGG GAGGTGCAGA ATTTGAAGAC ATGAGAGACC TCCTGGAGGC CCTGCTAGAT 
CACTTTGAAA TCGCTTCAGA GCCAGAGACG TCTGTCACTG GAGACAGGGT GGCCCTACTG 
AGTCATGCTC CCGTTGACTT CCTACCCAAC ACCCAGAGGA GTCCCGTTAG AACTGAGTTC 
AACCTAACCA CCTATAGCAG TAAGCACCTC ATGAAGAGAC ACGTGGAGCA AGCAGTCCAG 
CAGCTGAATG GAGATGCTTT CCTTGGTCAT GCCCTCAGGT GGGCACTGGA CAATGTCTTT 
CTAAACACGC CCAATCTGAG AAGGAACAAA GTCATATTTG TGATATCTGC TGGGGAAACC 
AGCCACCTGG ATGCGGAAAC CTTAAAGAAA GAGTCCCTGC GAGCCAAATG TCATGGGTAT 
GCCCTCTTTG TGTTTTCCTT GGGCCCTGAC TGGGATGACA AAGAACTGGA AGACCTGGCC 
AGCCACCCAC TTGACCAGCA CTTGATCCAG CTTGGCCGAA TTCATAAACC TGACCACGGA 
TATGGTGTAA AGTTTGTGAA GTCCTTTATA AACTCCATCA GGCATGGGAT AAACAAGTAT 
CCACCGGTCA ACTTTAAAGC CAAGTGCAGC AGACTCAGCT CGGCGGCTCC GAAGCTGTCC 
CCACGCCAGT TTCGAAGCTT TGTTCCGGGG CCACAGAAAG CTCTCCTCAA AGACCACACA 
CTGGAGGCAG CAAAGCTCTT TCAAGATAAA AAACGCCTTT CAAGTATCCT GAGAAGTGGC 
AGGGCTGCTC TTTCAAGTCA TTCCAGAAGC ACACGCTACT CCTTTAAACA GGGGAGAGAA 
GCGACAAAAG CTACTTCTAA ACTCGGTAAA CGAAGTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a6 ( XM_008757862.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008756084.1 Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X4, mRNA.

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CloneID	ORa18100
Clone ID Related Accession (Same CDS sequence)	XM_008757862.2 , XM_002727098.5 , XM_008757863.2 , XM_006226549.3
Accession Version	XM_008757862.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-6(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008757862.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGA TTTTGTTCCT GATGATGATT TGTTTACACG TTTGTGCGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGATGT GGTTTTTCTG GTGGACAGTT CCGATCACCT AGGGATGAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAA AAGCTAGTCA GCAGCCTCCC CGTAGAGGCC 
AACAAGTACC GTGTGGCCCT GGCCCAGTAC AGTGATGCTC TCCACAATGA GTTCCACCTA 
GGCGCCTTCA AGAACAGGAA CCCCATGCTG AACCACCTCA AGAAGAACTT CGGGTTCATC 
GGCGGCTCCC TGAAGATTGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAACG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCG 
GAGTCCGAGG ATGACGTAGA AGAGGCTTCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAGACCTGA AGGCCATGGC CACCTCTCAG 
TTTCATTTCA ACCTCAGGAC TGCCAGAGAC CTCAGCATGT TTGCCCCAAA CATGACACAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GCAATGACTG ATGACATTAT TGTAGAAGCT 
TGCCAAGGCC CATCTGTGGC CGATGTGGTA TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAAGAGAACC TAGATCATCT TAAAGCATTC CTGGGGGAAA GTGTCTCTGC CCTTGACATA 
AAGGAAAACT GCATGAGGGT TGGCCTGGTG GCCTATAGCA ACGAAACAAG GGTGATCAGT 
TCTCTGAGCA TGGGTGTCAA CAAGACGGAG GTCTTACAGC GCATACAGGA TCTGTCCCCT 
CACGTAGGGC AGGCCTACAC AGGAGCTGCC CTCAGAAAGA CGAGGAAGGA AGTCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTGCCTCAGA TTGCTGTGCT GGTGACCCAC 
AGAGCTTCAG ATGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
GTCTTTACCA TGGGCGTAGA GGGGGCTAAC CCAGAACAGC TGGAGAAGAT CGCATCCTAC 
CCTGCCGAGC AGTTCACCTC CAAACTGAGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAGCTGAG GAACCAAATC ACACACACAG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCAGC CTGTGTGGAC ACAGAGGAAG CGGATATCTA TCTGCTCATT 
GATGGTTCAG GGAACACGCA GCCCACAGAC TTCCATGAGA TGAAGATCTT CCTGTCCGAG 
GTGGTGGACA TGTTCAACAT CGCTCCCCAC AAGGTGCGAG TCGGGGCCGT GCAGTACGCC 
GACACCTGGG ACCTGGAATT TGAGATCTCT AAGTATACTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGACA ACATCAGGCA GATGGGTGGA AACACCAATA CGGGTGCAGC CCTGAACTTC 
ACCCTGACGC TGTTGCAAAG AGCAAAGAAG CAACGGGGAA ACAAAGTGCC ATGTCACCTG 
GTTGTCCTGA CAAACGGCAT GTCCCAGGAC AGCGTCCTGG GTCCCGCGCA TAAGCTGAGG 
GAGGAAAACA TCCGTGTGCA TGCGATCGGC GTCAAGGAAG CCAACCAAAC ACAGCTGCGG 
GAGATAGCGG GAGATGAAAA GCGAGTTTAT TACGTGCATG AATTCGATGC CTTGAGGGAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA TATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATTGGAC TCGAAAACTT CAGCAAGATG 
AAGGTGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGTGTGGTCC AGTTCAGCCA CGAAAACAGG GAGGAGTTTC AGCTCAACAC ATTCATGTCT 
CAAAATGACA TTGCCAACGC CATCGACCAA ATGGCTCACA TTGGAGAGAC AACCTTGACA 
GGCAGTGCGC TGACCTTTGT GTCTCAGTAC TTCAGTCCTG AAAAGGGGGC CCGGCCCAAT 
GTTAGGAAGT TCCTCATTCT CATCACGGAT GGCGAGGCTC AGGACATAGT AAAGGACCCA 
GCAGTAGCCC TTCGAAAAGA CGGTGTGATT ATCTATTCTG TGGGAGTGTT CGGCTCCAAT 
GTCACCCAGC TCGAGGAGAT CAGTGGGAAG CCGGAGATGG TTTTCTATGT TGAGAATTTT 
GACATCCTAC AGCATATTGA AGATGATCTC GTTCTGGGAA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTAGTG TTTGTCATCG ACAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCTCTG AAATATGCTG ATGACCCAGA GGTGCTGTTT 
TACCTGGATG AACTGGGCAC AAAGCTGGAG GTGATTTCAG TGCTCCAGAA TGACCAGCCC 
ATGGGTGGTA ACACTTACAC TGCTGAGGCC CTGGCTTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACTGATGGA 
GAATCTCATG ATGCAGAGAA GCTCAATGCC ACCGCCAAGG CCCTGAGGGA CAAAGGCATT 
CTGGTCCTAG CTGTGGGGAT TGCTGGCGCC AATACCTGGG AGCTCTTGGC CATGGCTGGG 
TCGAGTGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGGAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTCGATTGCG AAATTGAAAA AGTTGACCTC 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGAG 
TTTTTGGCAT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGAGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACATTCAC AGGGGAAAAG 
GAGATATCCA CACAGATTGA GGCCATCCAG CAGATCTTTG GTTACACCCA CATTGGAGAT 
GCACTCAGGA AGGTGAAGCA TTACTTTCGG CCAGACACGG GCAGCAGGAT CAACGCCGGT 
ACCCCTCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGATGAGGT GGCCCAGGCG 
GCTGAGGAGC TGAGACACAA GGGTGTGGAC ATCTACTCGG TGGGCATCGG GGACGTGGAT 
GACCAGCAGC TGATCCAGAT AACCGGGACA GCGGAGAAAA AACTGACGGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGTAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGTT TCGTGGATGT GGTGGTTGGA TTTGATATCT CAAGTCAGCG GAGAGGGCAG 
ACTTTGCTCG AAGGTCAGCC TTGGATGGGG ACCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAGGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ATGCTATGGA GAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTGAGCAGCC TGCAGGGTGT GACAGTGAAC GGCCCATCTC GCCTCAACCC AAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAACAAG TCCGCTGCTC GAGGGAAGGT GGTCCTTCTG 
TTTTCAGATG GATTGGATGA CGGCGTTGAA AAACTTGAAC AGAAATCTGA TGAACTCAGA 
AAGGAAGGCC TGAATGCCCT CATAACCATC GCTGTGGACG GAGCTGCCGA TTCCAGTGAC 
CTGGCTGACC TTCTCTACAT TGAATTTGGG AAAGGATTTG AGTACAGAAC ACAGTTCACA 
ATCGGAATGG GGAACCTTGG GAGTCACCTG TCAAAGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTTTGCTCTG CAAGTGCACA GGATGGGACG GCGCCATGGG GGATCCGGGC 
TCAGCAGGGG AAAAGGGACC CCCAGGATTT AAAGGCAGTG AAGGCTACCT GGGAGAAGAA 
GGCATTGCTG GAGAAAGAGG AGCCTTGGGA CCAATGGGAG AGCAAGGTAC AAAGGGATGC 
TTAGGTGCCA AAGGGCCCAA GGGAGCCCGA GGACTCAGCG GAGAAGAGGG TGAAGTTGGG 
GAAGATGGGC TTGACGGATT AAATGGAGAA CAGGGTGACA GTGGGATTCC TGGAAGAAGA 
GGAGAAAGGG GTGATGCGGG ATCTCAGGGA AGCCCAGGCA AGAGAGGGGC TTCTGGTGAC 
CGTGGACCGA AGGGCCTACG AGGAGATCCG GGAACTCCTG GACGTGATAA TAGCATACAG 
GGACCCAAGG GCTTGAAAGG AGACCCTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGGACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACATACAGGC 
CCACAGGGAA ATCCAGGCAT CACAGGCCCA GATGGACTTG AAGGTTCGCC CGGACTTAAG 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAGG CTTCGGAATG 
AAAGGTCCCC AGGGGCCTCC AGGACCCAAA GGACGAGCTG GGAACCAAGG CCATTGGGGA 
AGCCAAGGAA GCAAAGGAGA ACCTGGAGAC CTGGGGGAAA AGGGAGCTGC TGGCTTTCCA 
GGGCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCAGGAT ATGGTAGCAT TGGCCGAAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC CAAAGGGTGA TATTGGGGAC 
CCTGGCGATC CAGGAGAGGC TGGTCCCAAG GGAGCCAGAG GCAAAACGGT ATCTGCTGGG 
ATTCCAGGAG AGCCAGGATC CCCCGGGGAG CCAGGACCCC CTGGAAGGAA GGGCATGAAA 
GGAGCCCGAG GACTGGCTTC ATTTTCCACA TGTGACCTCA TTCAGTATGT GCGGGAACAC 
AGTCCTGTCA GACACGGAAA ACCTGAGTGC CCAGTGCATC CAACTGAGTT GGTGTTTGTC 
CTGGACCAGT CCCGGGATGT CACAGAACAG GACTTTGAGC GGATGAAGGG GATGATGGCC 
TCCTTAGTTA GGGACATCAA AGTTAGGGAT GCCAGCTGCC CCGTGGGTGC CCGCGTTGCC 
ATCCTAGCCT ACAACTCCCA CACCAGGCAC CTCATCCGCT TCTCGGATGC CTACAGGAAG 
GACCAGCTGC TCAGGGAAAT TAAAGCTCTT CCTTACGAGA AATCCTCCGA CAGCAGGGAG 
ATTGGCAAAG CCATGAGGTT TATCTCCAGG AATGTCTTCA AGAGAACCCT CCCAGGGGCT 
CACGTGAGAA GAATCGCCAC GTTCTTTAGC AGTGGCCAGT CAGCCGATGT CCAGTCCATT 
ACCACAGCTG CCATGGAGTT CAGTGCCCTG GACATCATAC CAGTGGTGAT TGCATTCAGC 
AACGTGCCCT CGGTCAAGCG TGCCTTTTCG ATTGATGACA CTGGTACATT CCAAGTCCTT 
GTGGTTCCCT CCAGGCCTGA CCAAGTGCCA GCGTTAGAGA GACTCCAGCG GTGCACTTTC 
TGCTATGACC TATGCAAGCC AGATGCTTCT TGTGACCAAG CCAAACCCCC CATTCAGTCC 
TACCTGGATG CTGCATTCCT GCTGGATAGC TCCTGGCATG TGGGAGGTGC AGAATTTGAA 
GACATGAGAG ACCTCCTGGA GGCCCTGCTA GATCACTTTG AAATCGCTTC AGAGCCAGAG 
ACGTCTGTCA CTGGAGACAG GGTGGCCCTA CTGAGTCATG CTCCCGTTGA CTTCCTACCC 
AACACCCAGA GGAGTCCCGT TAGAACTGAG TTCAACCTAA CCACCTATAG CAGTAAGCAC 
CTCATGAAGA GACACGTGGA GCAAGCAGTC CAGCAGCTGA ATGGAGATGC TTTCCTTGGT 
CATGCCCTCA GGTGGGCACT GGACAATGTC TTTCTAAACA CGCCCAATCT GAGAAGGAAC 
AAAGTCATAT TTGTGATATC TGCTGGGGAA ACCAGCCACC TGGATGCGGA AACCTTAAAG 
AAAGAGTCCC TGCGAGCCAA ATGTCATGGG TATGCCCTCT TTGTGTTTTC CTTGGGCCCT 
GACTGGGATG ACAAAGAACT GGAAGACCTG GCCAGCCACC CACTTGACCA GCACTTGATC 
CAGCTTGGCC GAATTCATAA ACCTGACCAC GGATATGGTG TAAAGTTTGT GAAGTCCTTT 
ATAAACTCCA TCAGGCATGG GATAAACAAG TATCCACCGG TCAACTTTAA AGCCAAGTGC 
AGCAGACTCA GCTCGGCGGC TCCGAAGCTG TCCCCACGCC AGTTTCGAAG CTTTGTTCCG 
GGGCCACAGA AAGCTCTCCT CAAAGACCAC ACACTGGAGG CAGCAAAGCT CTTTCAAGAT 
AAAAAACGCC TTTCAAGTAT CCTGAGAAGT GGCAGGGCTG CTCTTTCAAG TCATTCCAGA 
AGCACACGCT ACTCCTTTAA ACAGGGGAGA GAAGCGACAA AAGCTACTTC TAAACTCGGT 
AAACGAAGTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008756084.1
CDS	199..6993
Translation	MLLILFLMMICLHVCANQDSGPEYADVVFLVDSSDHLGMKSFPLVKTFIQKLVSSLPVEANKYRVALAQYSDALHNEFHLGAFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEASKALREDGVKIISVGVQKASEEDLKAMATSQFHFNLRTARDLSMFAPNMTQIIKDVTQYREAMTDDIIVEACQGPSVADVVFLLDMAINGSQENLDHLKAFLGESVSALDIKENCMRVGLVAYSNETRVISSLSMGVNKTEVLQRIQDLSPHVGQAYTGAALRKTRKEVFSAQRGSRKNQGVPQIAVLVTHRASDDNVTKAAVNLRREGVTVFTMGVEGANPEQLEKIASYPAEQFTSKLSNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGNTQPTDFHEMKIFLSEVVDMFNIAPHKVRVGAVQYADTWDLEFEISKYTNKPDLGKAIDNIRQMGGNTNTGAALNFTLTLLQRAKKQRGNKVPCHLVVLTNGMSQDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGDEKRVYYVHEFDALRDIRNQVVQEICAEEACRDMKADIMFLVDSSGSIGLENFSKMKVFMKNLVSKSQIGADRVQIGVVQFSHENREEFQLNTFMSQNDIANAIDQMAHIGETTLTGSALTFVSQYFSPEKGARPNVRKFLILITDGEAQDIVKDPAVALRKDGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVISVLQNDQPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNATAKALRDKGILVLAVGIAGANTWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFQKMKEFLASVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEKEISTQIEAIQQIFGYTHIGDALRKVKHYFRPDTGSRINAGTPQVLLVLTDGRSQDEVAQAAEELRHKGVDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVKKRIVRNICTSGGESNCFVDVVVGFDISSQRRGQTLLEGQPWMGTYLQDLLRAISSLNGVSCEVGTETQVSIAFQVTNAMERYPSKFEIYSENILSSLQGVTVNGPSRLNPNLLSSLWDTFQNKSAARGKVVLLFSDGLDDGVEKLEQKSDELRKEGLNALITIAVDGAADSSDLADLLYIEFGKGFEYRTQFTIGMGNLGSHLSKQLINVAERTCCCLLCKCTGWDGAMGDPGSAGEKGPPGFKGSEGYLGEEGIAGERGALGPMGEQGTKGCLGAKGPKGARGLSGEEGEVGEDGLDGLNGEQGDSGIPGRRGERGDAGSQGSPGKRGASGDRGPKGLRGDPGTPGRDNSIQGPKGLKGDPGRQGRRGWPGSPGTPGSRRKMVVHGRRGHTGPQGNPGITGPDGLEGSPGLKGPQGPRGEVGEKGEKGGFGMKGPQGPPGPKGRAGNQGHWGSQGSKGEPGDLGEKGAAGFPGPRGLQGDDGSPGYGSIGRKGTKGQEGFPGESGPKGDIGDPGDPGEAGPKGARGKTVSAGIPGEPGSPGEPGPPGRKGMKGARGLASFSTCDLIQYVREHSPVRHGKPECPVHPTELVFVLDQSRDVTEQDFERMKGMMASLVRDIKVRDASCPVGARVAILAYNSHTRHLIRFSDAYRKDQLLREIKALPYEKSSDSREIGKAMRFISRNVFKRTLPGAHVRRIATFFSSGQSADVQSITTAAMEFSALDIIPVVIAFSNVPSVKRAFSIDDTGTFQVLVVPSRPDQVPALERLQRCTFCYDLCKPDASCDQAKPPIQSYLDAAFLLDSSWHVGGAEFEDMRDLLEALLDHFEIASEPETSVTGDRVALLSHAPVDFLPNTQRSPVRTEFNLTTYSSKHLMKRHVEQAVQQLNGDAFLGHALRWALDNVFLNTPNLRRNKVIFVISAGETSHLDAETLKKESLRAKCHGYALFVFSLGPDWDDKELEDLASHPLDQHLIQLGRIHKPDHGYGVKFVKSFINSIRHGINKYPPVNFKAKCSRLSSAAPKLSPRQFRSFVPGPQKALLKDHTLEAAKLFQDKKRLSSILRSGRAALSSHSRSTRYSFKQGREATKATSKLGKRSD

Target ORF information:

RefSeq Version	XM_008757862.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008757862.2

ORF Insert Sequence:

ATGCTGCTGA TTTTGTTCCT GATGATGATT TGTTTACACG TTTGTGCGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGATGT GGTTTTTCTG GTGGACAGTT CCGATCACCT AGGGATGAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAA AAGCTAGTCA GCAGCCTCCC CGTAGAGGCC 
AACAAGTACC GTGTGGCCCT GGCCCAGTAC AGTGATGCTC TCCACAATGA GTTCCACCTA 
GGCGCCTTCA AGAACAGGAA CCCCATGCTG AACCACCTCA AGAAGAACTT CGGGTTCATC 
GGCGGCTCCC TGAAGATTGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAACG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCG 
GAGTCCGAGG ATGACGTAGA AGAGGCTTCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAGACCTGA AGGCCATGGC CACCTCTCAG 
TTTCATTTCA ACCTCAGGAC TGCCAGAGAC CTCAGCATGT TTGCCCCAAA CATGACACAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GCAATGACTG ATGACATTAT TGTAGAAGCT 
TGCCAAGGCC CATCTGTGGC CGATGTGGTA TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAAGAGAACC TAGATCATCT TAAAGCATTC CTGGGGGAAA GTGTCTCTGC CCTTGACATA 
AAGGAAAACT GCATGAGGGT TGGCCTGGTG GCCTATAGCA ACGAAACAAG GGTGATCAGT 
TCTCTGAGCA TGGGTGTCAA CAAGACGGAG GTCTTACAGC GCATACAGGA TCTGTCCCCT 
CACGTAGGGC AGGCCTACAC AGGAGCTGCC CTCAGAAAGA CGAGGAAGGA AGTCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTGCCTCAGA TTGCTGTGCT GGTGACCCAC 
AGAGCTTCAG ATGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
GTCTTTACCA TGGGCGTAGA GGGGGCTAAC CCAGAACAGC TGGAGAAGAT CGCATCCTAC 
CCTGCCGAGC AGTTCACCTC CAAACTGAGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAGCTGAG GAACCAAATC ACACACACAG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCAGC CTGTGTGGAC ACAGAGGAAG CGGATATCTA TCTGCTCATT 
GATGGTTCAG GGAACACGCA GCCCACAGAC TTCCATGAGA TGAAGATCTT CCTGTCCGAG 
GTGGTGGACA TGTTCAACAT CGCTCCCCAC AAGGTGCGAG TCGGGGCCGT GCAGTACGCC 
GACACCTGGG ACCTGGAATT TGAGATCTCT AAGTATACTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGACA ACATCAGGCA GATGGGTGGA AACACCAATA CGGGTGCAGC CCTGAACTTC 
ACCCTGACGC TGTTGCAAAG AGCAAAGAAG CAACGGGGAA ACAAAGTGCC ATGTCACCTG 
GTTGTCCTGA CAAACGGCAT GTCCCAGGAC AGCGTCCTGG GTCCCGCGCA TAAGCTGAGG 
GAGGAAAACA TCCGTGTGCA TGCGATCGGC GTCAAGGAAG CCAACCAAAC ACAGCTGCGG 
GAGATAGCGG GAGATGAAAA GCGAGTTTAT TACGTGCATG AATTCGATGC CTTGAGGGAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA TATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATTGGAC TCGAAAACTT CAGCAAGATG 
AAGGTGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGTGTGGTCC AGTTCAGCCA CGAAAACAGG GAGGAGTTTC AGCTCAACAC ATTCATGTCT 
CAAAATGACA TTGCCAACGC CATCGACCAA ATGGCTCACA TTGGAGAGAC AACCTTGACA 
GGCAGTGCGC TGACCTTTGT GTCTCAGTAC TTCAGTCCTG AAAAGGGGGC CCGGCCCAAT 
GTTAGGAAGT TCCTCATTCT CATCACGGAT GGCGAGGCTC AGGACATAGT AAAGGACCCA 
GCAGTAGCCC TTCGAAAAGA CGGTGTGATT ATCTATTCTG TGGGAGTGTT CGGCTCCAAT 
GTCACCCAGC TCGAGGAGAT CAGTGGGAAG CCGGAGATGG TTTTCTATGT TGAGAATTTT 
GACATCCTAC AGCATATTGA AGATGATCTC GTTCTGGGAA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTAGTG TTTGTCATCG ACAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCTCTG AAATATGCTG ATGACCCAGA GGTGCTGTTT 
TACCTGGATG AACTGGGCAC AAAGCTGGAG GTGATTTCAG TGCTCCAGAA TGACCAGCCC 
ATGGGTGGTA ACACTTACAC TGCTGAGGCC CTGGCTTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACTGATGGA 
GAATCTCATG ATGCAGAGAA GCTCAATGCC ACCGCCAAGG CCCTGAGGGA CAAAGGCATT 
CTGGTCCTAG CTGTGGGGAT TGCTGGCGCC AATACCTGGG AGCTCTTGGC CATGGCTGGG 
TCGAGTGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGGAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTCGATTGCG AAATTGAAAA AGTTGACCTC 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGAG 
TTTTTGGCAT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGAGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACATTCAC AGGGGAAAAG 
GAGATATCCA CACAGATTGA GGCCATCCAG CAGATCTTTG GTTACACCCA CATTGGAGAT 
GCACTCAGGA AGGTGAAGCA TTACTTTCGG CCAGACACGG GCAGCAGGAT CAACGCCGGT 
ACCCCTCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGATGAGGT GGCCCAGGCG 
GCTGAGGAGC TGAGACACAA GGGTGTGGAC ATCTACTCGG TGGGCATCGG GGACGTGGAT 
GACCAGCAGC TGATCCAGAT AACCGGGACA GCGGAGAAAA AACTGACGGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGTAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGTT TCGTGGATGT GGTGGTTGGA TTTGATATCT CAAGTCAGCG GAGAGGGCAG 
ACTTTGCTCG AAGGTCAGCC TTGGATGGGG ACCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAGGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ATGCTATGGA GAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTGAGCAGCC TGCAGGGTGT GACAGTGAAC GGCCCATCTC GCCTCAACCC AAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAACAAG TCCGCTGCTC GAGGGAAGGT GGTCCTTCTG 
TTTTCAGATG GATTGGATGA CGGCGTTGAA AAACTTGAAC AGAAATCTGA TGAACTCAGA 
AAGGAAGGCC TGAATGCCCT CATAACCATC GCTGTGGACG GAGCTGCCGA TTCCAGTGAC 
CTGGCTGACC TTCTCTACAT TGAATTTGGG AAAGGATTTG AGTACAGAAC ACAGTTCACA 
ATCGGAATGG GGAACCTTGG GAGTCACCTG TCAAAGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTTTGCTCTG CAAGTGCACA GGATGGGACG GCGCCATGGG GGATCCGGGC 
TCAGCAGGGG AAAAGGGACC CCCAGGATTT AAAGGCAGTG AAGGCTACCT GGGAGAAGAA 
GGCATTGCTG GAGAAAGAGG AGCCTTGGGA CCAATGGGAG AGCAAGGTAC AAAGGGATGC 
TTAGGTGCCA AAGGGCCCAA GGGAGCCCGA GGACTCAGCG GAGAAGAGGG TGAAGTTGGG 
GAAGATGGGC TTGACGGATT AAATGGAGAA CAGGGTGACA GTGGGATTCC TGGAAGAAGA 
GGAGAAAGGG GTGATGCGGG ATCTCAGGGA AGCCCAGGCA AGAGAGGGGC TTCTGGTGAC 
CGTGGACCGA AGGGCCTACG AGGAGATCCG GGAACTCCTG GACGTGATAA TAGCATACAG 
GGACCCAAGG GCTTGAAAGG AGACCCTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGGACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACATACAGGC 
CCACAGGGAA ATCCAGGCAT CACAGGCCCA GATGGACTTG AAGGTTCGCC CGGACTTAAG 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAGG CTTCGGAATG 
AAAGGTCCCC AGGGGCCTCC AGGACCCAAA GGACGAGCTG GGAACCAAGG CCATTGGGGA 
AGCCAAGGAA GCAAAGGAGA ACCTGGAGAC CTGGGGGAAA AGGGAGCTGC TGGCTTTCCA 
GGGCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCAGGAT ATGGTAGCAT TGGCCGAAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC CAAAGGGTGA TATTGGGGAC 
CCTGGCGATC CAGGAGAGGC TGGTCCCAAG GGAGCCAGAG GCAAAACGGT ATCTGCTGGG 
ATTCCAGGAG AGCCAGGATC CCCCGGGGAG CCAGGACCCC CTGGAAGGAA GGGCATGAAA 
GGAGCCCGAG GACTGGCTTC ATTTTCCACA TGTGACCTCA TTCAGTATGT GCGGGAACAC 
AGTCCTGTCA GACACGGAAA ACCTGAGTGC CCAGTGCATC CAACTGAGTT GGTGTTTGTC 
CTGGACCAGT CCCGGGATGT CACAGAACAG GACTTTGAGC GGATGAAGGG GATGATGGCC 
TCCTTAGTTA GGGACATCAA AGTTAGGGAT GCCAGCTGCC CCGTGGGTGC CCGCGTTGCC 
ATCCTAGCCT ACAACTCCCA CACCAGGCAC CTCATCCGCT TCTCGGATGC CTACAGGAAG 
GACCAGCTGC TCAGGGAAAT TAAAGCTCTT CCTTACGAGA AATCCTCCGA CAGCAGGGAG 
ATTGGCAAAG CCATGAGGTT TATCTCCAGG AATGTCTTCA AGAGAACCCT CCCAGGGGCT 
CACGTGAGAA GAATCGCCAC GTTCTTTAGC AGTGGCCAGT CAGCCGATGT CCAGTCCATT 
ACCACAGCTG CCATGGAGTT CAGTGCCCTG GACATCATAC CAGTGGTGAT TGCATTCAGC 
AACGTGCCCT CGGTCAAGCG TGCCTTTTCG ATTGATGACA CTGGTACATT CCAAGTCCTT 
GTGGTTCCCT CCAGGCCTGA CCAAGTGCCA GCGTTAGAGA GACTCCAGCG GTGCACTTTC 
TGCTATGACC TATGCAAGCC AGATGCTTCT TGTGACCAAG CCAAACCCCC CATTCAGTCC 
TACCTGGATG CTGCATTCCT GCTGGATAGC TCCTGGCATG TGGGAGGTGC AGAATTTGAA 
GACATGAGAG ACCTCCTGGA GGCCCTGCTA GATCACTTTG AAATCGCTTC AGAGCCAGAG 
ACGTCTGTCA CTGGAGACAG GGTGGCCCTA CTGAGTCATG CTCCCGTTGA CTTCCTACCC 
AACACCCAGA GGAGTCCCGT TAGAACTGAG TTCAACCTAA CCACCTATAG CAGTAAGCAC 
CTCATGAAGA GACACGTGGA GCAAGCAGTC CAGCAGCTGA ATGGAGATGC TTTCCTTGGT 
CATGCCCTCA GGTGGGCACT GGACAATGTC TTTCTAAACA CGCCCAATCT GAGAAGGAAC 
AAAGTCATAT TTGTGATATC TGCTGGGGAA ACCAGCCACC TGGATGCGGA AACCTTAAAG 
AAAGAGTCCC TGCGAGCCAA ATGTCATGGG TATGCCCTCT TTGTGTTTTC CTTGGGCCCT 
GACTGGGATG ACAAAGAACT GGAAGACCTG GCCAGCCACC CACTTGACCA GCACTTGATC 
CAGCTTGGCC GAATTCATAA ACCTGACCAC GGATATGGTG TAAAGTTTGT GAAGTCCTTT 
ATAAACTCCA TCAGGCATGG GATAAACAAG TATCCACCGG TCAACTTTAA AGCCAAGTGC 
AGCAGACTCA GCTCGGCGGC TCCGAAGCTG TCCCCACGCC AGTTTCGAAG CTTTGTTCCG 
GGGCCACAGA AAGCTCTCCT CAAAGACCAC ACACTGGAGG CAGCAAAGCT CTTTCAAGAT 
AAAAAACGCC TTTCAAGTAT CCTGAGAAGT GGCAGGGCTG CTCTTTCAAG TCATTCCAGA 
AGCACACGCT ACTCCTTTAA ACAGGGGAGA GAAGCGACAA AAGCTACTTC TAAACTCGGT 
AAACGAAGTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a6 ( XM_002727098.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_002727144.1 Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X3, mRNA.

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CloneID	ORa18100
Clone ID Related Accession (Same CDS sequence)	XM_008757862.2 , XM_002727098.5 , XM_008757863.2 , XM_006226549.3
Accession Version	XM_002727098.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-6(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_002727098.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGA TTTTGTTCCT GATGATGATT TGTTTACACG TTTGTGCGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGATGT GGTTTTTCTG GTGGACAGTT CCGATCACCT AGGGATGAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAA AAGCTAGTCA GCAGCCTCCC CGTAGAGGCC 
AACAAGTACC GTGTGGCCCT GGCCCAGTAC AGTGATGCTC TCCACAATGA GTTCCACCTA 
GGCGCCTTCA AGAACAGGAA CCCCATGCTG AACCACCTCA AGAAGAACTT CGGGTTCATC 
GGCGGCTCCC TGAAGATTGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAACG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCG 
GAGTCCGAGG ATGACGTAGA AGAGGCTTCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAGACCTGA AGGCCATGGC CACCTCTCAG 
TTTCATTTCA ACCTCAGGAC TGCCAGAGAC CTCAGCATGT TTGCCCCAAA CATGACACAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GCAATGACTG ATGACATTAT TGTAGAAGCT 
TGCCAAGGCC CATCTGTGGC CGATGTGGTA TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAAGAGAACC TAGATCATCT TAAAGCATTC CTGGGGGAAA GTGTCTCTGC CCTTGACATA 
AAGGAAAACT GCATGAGGGT TGGCCTGGTG GCCTATAGCA ACGAAACAAG GGTGATCAGT 
TCTCTGAGCA TGGGTGTCAA CAAGACGGAG GTCTTACAGC GCATACAGGA TCTGTCCCCT 
CACGTAGGGC AGGCCTACAC AGGAGCTGCC CTCAGAAAGA CGAGGAAGGA AGTCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTGCCTCAGA TTGCTGTGCT GGTGACCCAC 
AGAGCTTCAG ATGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
GTCTTTACCA TGGGCGTAGA GGGGGCTAAC CCAGAACAGC TGGAGAAGAT CGCATCCTAC 
CCTGCCGAGC AGTTCACCTC CAAACTGAGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAGCTGAG GAACCAAATC ACACACACAG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCAGC CTGTGTGGAC ACAGAGGAAG CGGATATCTA TCTGCTCATT 
GATGGTTCAG GGAACACGCA GCCCACAGAC TTCCATGAGA TGAAGATCTT CCTGTCCGAG 
GTGGTGGACA TGTTCAACAT CGCTCCCCAC AAGGTGCGAG TCGGGGCCGT GCAGTACGCC 
GACACCTGGG ACCTGGAATT TGAGATCTCT AAGTATACTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGACA ACATCAGGCA GATGGGTGGA AACACCAATA CGGGTGCAGC CCTGAACTTC 
ACCCTGACGC TGTTGCAAAG AGCAAAGAAG CAACGGGGAA ACAAAGTGCC ATGTCACCTG 
GTTGTCCTGA CAAACGGCAT GTCCCAGGAC AGCGTCCTGG GTCCCGCGCA TAAGCTGAGG 
GAGGAAAACA TCCGTGTGCA TGCGATCGGC GTCAAGGAAG CCAACCAAAC ACAGCTGCGG 
GAGATAGCGG GAGATGAAAA GCGAGTTTAT TACGTGCATG AATTCGATGC CTTGAGGGAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA TATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATTGGAC TCGAAAACTT CAGCAAGATG 
AAGGTGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGTGTGGTCC AGTTCAGCCA CGAAAACAGG GAGGAGTTTC AGCTCAACAC ATTCATGTCT 
CAAAATGACA TTGCCAACGC CATCGACCAA ATGGCTCACA TTGGAGAGAC AACCTTGACA 
GGCAGTGCGC TGACCTTTGT GTCTCAGTAC TTCAGTCCTG AAAAGGGGGC CCGGCCCAAT 
GTTAGGAAGT TCCTCATTCT CATCACGGAT GGCGAGGCTC AGGACATAGT AAAGGACCCA 
GCAGTAGCCC TTCGAAAAGA CGGTGTGATT ATCTATTCTG TGGGAGTGTT CGGCTCCAAT 
GTCACCCAGC TCGAGGAGAT CAGTGGGAAG CCGGAGATGG TTTTCTATGT TGAGAATTTT 
GACATCCTAC AGCATATTGA AGATGATCTC GTTCTGGGAA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTAGTG TTTGTCATCG ACAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCTCTG AAATATGCTG ATGACCCAGA GGTGCTGTTT 
TACCTGGATG AACTGGGCAC AAAGCTGGAG GTGATTTCAG TGCTCCAGAA TGACCAGCCC 
ATGGGTGGTA ACACTTACAC TGCTGAGGCC CTGGCTTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACTGATGGA 
GAATCTCATG ATGCAGAGAA GCTCAATGCC ACCGCCAAGG CCCTGAGGGA CAAAGGCATT 
CTGGTCCTAG CTGTGGGGAT TGCTGGCGCC AATACCTGGG AGCTCTTGGC CATGGCTGGG 
TCGAGTGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGGAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTCGATTGCG AAATTGAAAA AGTTGACCTC 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGAG 
TTTTTGGCAT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGAGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACATTCAC AGGGGAAAAG 
GAGATATCCA CACAGATTGA GGCCATCCAG CAGATCTTTG GTTACACCCA CATTGGAGAT 
GCACTCAGGA AGGTGAAGCA TTACTTTCGG CCAGACACGG GCAGCAGGAT CAACGCCGGT 
ACCCCTCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGATGAGGT GGCCCAGGCG 
GCTGAGGAGC TGAGACACAA GGGTGTGGAC ATCTACTCGG TGGGCATCGG GGACGTGGAT 
GACCAGCAGC TGATCCAGAT AACCGGGACA GCGGAGAAAA AACTGACGGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGTAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGTT TCGTGGATGT GGTGGTTGGA TTTGATATCT CAAGTCAGCG GAGAGGGCAG 
ACTTTGCTCG AAGGTCAGCC TTGGATGGGG ACCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAGGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ATGCTATGGA GAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTGAGCAGCC TGCAGGGTGT GACAGTGAAC GGCCCATCTC GCCTCAACCC AAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAACAAG TCCGCTGCTC GAGGGAAGGT GGTCCTTCTG 
TTTTCAGATG GATTGGATGA CGGCGTTGAA AAACTTGAAC AGAAATCTGA TGAACTCAGA 
AAGGAAGGCC TGAATGCCCT CATAACCATC GCTGTGGACG GAGCTGCCGA TTCCAGTGAC 
CTGGCTGACC TTCTCTACAT TGAATTTGGG AAAGGATTTG AGTACAGAAC ACAGTTCACA 
ATCGGAATGG GGAACCTTGG GAGTCACCTG TCAAAGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTTTGCTCTG CAAGTGCACA GGATGGGACG GCGCCATGGG GGATCCGGGC 
TCAGCAGGGG AAAAGGGACC CCCAGGATTT AAAGGCAGTG AAGGCTACCT GGGAGAAGAA 
GGCATTGCTG GAGAAAGAGG AGCCTTGGGA CCAATGGGAG AGCAAGGTAC AAAGGGATGC 
TTAGGTGCCA AAGGGCCCAA GGGAGCCCGA GGACTCAGCG GAGAAGAGGG TGAAGTTGGG 
GAAGATGGGC TTGACGGATT AAATGGAGAA CAGGGTGACA GTGGGATTCC TGGAAGAAGA 
GGAGAAAGGG GTGATGCGGG ATCTCAGGGA AGCCCAGGCA AGAGAGGGGC TTCTGGTGAC 
CGTGGACCGA AGGGCCTACG AGGAGATCCG GGAACTCCTG GACGTGATAA TAGCATACAG 
GGACCCAAGG GCTTGAAAGG AGACCCTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGGACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACATACAGGC 
CCACAGGGAA ATCCAGGCAT CACAGGCCCA GATGGACTTG AAGGTTCGCC CGGACTTAAG 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAGG CTTCGGAATG 
AAAGGTCCCC AGGGGCCTCC AGGACCCAAA GGACGAGCTG GGAACCAAGG CCATTGGGGA 
AGCCAAGGAA GCAAAGGAGA ACCTGGAGAC CTGGGGGAAA AGGGAGCTGC TGGCTTTCCA 
GGGCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCAGGAT ATGGTAGCAT TGGCCGAAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC CAAAGGGTGA TATTGGGGAC 
CCTGGCGATC CAGGAGAGGC TGGTCCCAAG GGAGCCAGAG GCAAAACGGT ATCTGCTGGG 
ATTCCAGGAG AGCCAGGATC CCCCGGGGAG CCAGGACCCC CTGGAAGGAA GGGCATGAAA 
GGAGCCCGAG GACTGGCTTC ATTTTCCACA TGTGACCTCA TTCAGTATGT GCGGGAACAC 
AGTCCTGTCA GACACGGAAA ACCTGAGTGC CCAGTGCATC CAACTGAGTT GGTGTTTGTC 
CTGGACCAGT CCCGGGATGT CACAGAACAG GACTTTGAGC GGATGAAGGG GATGATGGCC 
TCCTTAGTTA GGGACATCAA AGTTAGGGAT GCCAGCTGCC CCGTGGGTGC CCGCGTTGCC 
ATCCTAGCCT ACAACTCCCA CACCAGGCAC CTCATCCGCT TCTCGGATGC CTACAGGAAG 
GACCAGCTGC TCAGGGAAAT TAAAGCTCTT CCTTACGAGA AATCCTCCGA CAGCAGGGAG 
ATTGGCAAAG CCATGAGGTT TATCTCCAGG AATGTCTTCA AGAGAACCCT CCCAGGGGCT 
CACGTGAGAA GAATCGCCAC GTTCTTTAGC AGTGGCCAGT CAGCCGATGT CCAGTCCATT 
ACCACAGCTG CCATGGAGTT CAGTGCCCTG GACATCATAC CAGTGGTGAT TGCATTCAGC 
AACGTGCCCT CGGTCAAGCG TGCCTTTTCG ATTGATGACA CTGGTACATT CCAAGTCCTT 
GTGGTTCCCT CCAGGCCTGA CCAAGTGCCA GCGTTAGAGA GACTCCAGCG GTGCACTTTC 
TGCTATGACC TATGCAAGCC AGATGCTTCT TGTGACCAAG CCAAACCCCC CATTCAGTCC 
TACCTGGATG CTGCATTCCT GCTGGATAGC TCCTGGCATG TGGGAGGTGC AGAATTTGAA 
GACATGAGAG ACCTCCTGGA GGCCCTGCTA GATCACTTTG AAATCGCTTC AGAGCCAGAG 
ACGTCTGTCA CTGGAGACAG GGTGGCCCTA CTGAGTCATG CTCCCGTTGA CTTCCTACCC 
AACACCCAGA GGAGTCCCGT TAGAACTGAG TTCAACCTAA CCACCTATAG CAGTAAGCAC 
CTCATGAAGA GACACGTGGA GCAAGCAGTC CAGCAGCTGA ATGGAGATGC TTTCCTTGGT 
CATGCCCTCA GGTGGGCACT GGACAATGTC TTTCTAAACA CGCCCAATCT GAGAAGGAAC 
AAAGTCATAT TTGTGATATC TGCTGGGGAA ACCAGCCACC TGGATGCGGA AACCTTAAAG 
AAAGAGTCCC TGCGAGCCAA ATGTCATGGG TATGCCCTCT TTGTGTTTTC CTTGGGCCCT 
GACTGGGATG ACAAAGAACT GGAAGACCTG GCCAGCCACC CACTTGACCA GCACTTGATC 
CAGCTTGGCC GAATTCATAA ACCTGACCAC GGATATGGTG TAAAGTTTGT GAAGTCCTTT 
ATAAACTCCA TCAGGCATGG GATAAACAAG TATCCACCGG TCAACTTTAA AGCCAAGTGC 
AGCAGACTCA GCTCGGCGGC TCCGAAGCTG TCCCCACGCC AGTTTCGAAG CTTTGTTCCG 
GGGCCACAGA AAGCTCTCCT CAAAGACCAC ACACTGGAGG CAGCAAAGCT CTTTCAAGAT 
AAAAAACGCC TTTCAAGTAT CCTGAGAAGT GGCAGGGCTG CTCTTTCAAG TCATTCCAGA 
AGCACACGCT ACTCCTTTAA ACAGGGGAGA GAAGCGACAA AAGCTACTTC TAAACTCGGT 
AAACGAAGTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002727144.1
CDS	247..7041
Translation	MLLILFLMMICLHVCANQDSGPEYADVVFLVDSSDHLGMKSFPLVKTFIQKLVSSLPVEANKYRVALAQYSDALHNEFHLGAFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEASKALREDGVKIISVGVQKASEEDLKAMATSQFHFNLRTARDLSMFAPNMTQIIKDVTQYREAMTDDIIVEACQGPSVADVVFLLDMAINGSQENLDHLKAFLGESVSALDIKENCMRVGLVAYSNETRVISSLSMGVNKTEVLQRIQDLSPHVGQAYTGAALRKTRKEVFSAQRGSRKNQGVPQIAVLVTHRASDDNVTKAAVNLRREGVTVFTMGVEGANPEQLEKIASYPAEQFTSKLSNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGNTQPTDFHEMKIFLSEVVDMFNIAPHKVRVGAVQYADTWDLEFEISKYTNKPDLGKAIDNIRQMGGNTNTGAALNFTLTLLQRAKKQRGNKVPCHLVVLTNGMSQDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGDEKRVYYVHEFDALRDIRNQVVQEICAEEACRDMKADIMFLVDSSGSIGLENFSKMKVFMKNLVSKSQIGADRVQIGVVQFSHENREEFQLNTFMSQNDIANAIDQMAHIGETTLTGSALTFVSQYFSPEKGARPNVRKFLILITDGEAQDIVKDPAVALRKDGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVISVLQNDQPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNATAKALRDKGILVLAVGIAGANTWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFQKMKEFLASVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEKEISTQIEAIQQIFGYTHIGDALRKVKHYFRPDTGSRINAGTPQVLLVLTDGRSQDEVAQAAEELRHKGVDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVKKRIVRNICTSGGESNCFVDVVVGFDISSQRRGQTLLEGQPWMGTYLQDLLRAISSLNGVSCEVGTETQVSIAFQVTNAMERYPSKFEIYSENILSSLQGVTVNGPSRLNPNLLSSLWDTFQNKSAARGKVVLLFSDGLDDGVEKLEQKSDELRKEGLNALITIAVDGAADSSDLADLLYIEFGKGFEYRTQFTIGMGNLGSHLSKQLINVAERTCCCLLCKCTGWDGAMGDPGSAGEKGPPGFKGSEGYLGEEGIAGERGALGPMGEQGTKGCLGAKGPKGARGLSGEEGEVGEDGLDGLNGEQGDSGIPGRRGERGDAGSQGSPGKRGASGDRGPKGLRGDPGTPGRDNSIQGPKGLKGDPGRQGRRGWPGSPGTPGSRRKMVVHGRRGHTGPQGNPGITGPDGLEGSPGLKGPQGPRGEVGEKGEKGGFGMKGPQGPPGPKGRAGNQGHWGSQGSKGEPGDLGEKGAAGFPGPRGLQGDDGSPGYGSIGRKGTKGQEGFPGESGPKGDIGDPGDPGEAGPKGARGKTVSAGIPGEPGSPGEPGPPGRKGMKGARGLASFSTCDLIQYVREHSPVRHGKPECPVHPTELVFVLDQSRDVTEQDFERMKGMMASLVRDIKVRDASCPVGARVAILAYNSHTRHLIRFSDAYRKDQLLREIKALPYEKSSDSREIGKAMRFISRNVFKRTLPGAHVRRIATFFSSGQSADVQSITTAAMEFSALDIIPVVIAFSNVPSVKRAFSIDDTGTFQVLVVPSRPDQVPALERLQRCTFCYDLCKPDASCDQAKPPIQSYLDAAFLLDSSWHVGGAEFEDMRDLLEALLDHFEIASEPETSVTGDRVALLSHAPVDFLPNTQRSPVRTEFNLTTYSSKHLMKRHVEQAVQQLNGDAFLGHALRWALDNVFLNTPNLRRNKVIFVISAGETSHLDAETLKKESLRAKCHGYALFVFSLGPDWDDKELEDLASHPLDQHLIQLGRIHKPDHGYGVKFVKSFINSIRHGINKYPPVNFKAKCSRLSSAAPKLSPRQFRSFVPGPQKALLKDHTLEAAKLFQDKKRLSSILRSGRAALSSHSRSTRYSFKQGREATKATSKLGKRSD

Target ORF information:

RefSeq Version	XM_002727098.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002727098.5

ORF Insert Sequence:

ATGCTGCTGA TTTTGTTCCT GATGATGATT TGTTTACACG TTTGTGCGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGATGT GGTTTTTCTG GTGGACAGTT CCGATCACCT AGGGATGAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAA AAGCTAGTCA GCAGCCTCCC CGTAGAGGCC 
AACAAGTACC GTGTGGCCCT GGCCCAGTAC AGTGATGCTC TCCACAATGA GTTCCACCTA 
GGCGCCTTCA AGAACAGGAA CCCCATGCTG AACCACCTCA AGAAGAACTT CGGGTTCATC 
GGCGGCTCCC TGAAGATTGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAACG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCG 
GAGTCCGAGG ATGACGTAGA AGAGGCTTCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAGACCTGA AGGCCATGGC CACCTCTCAG 
TTTCATTTCA ACCTCAGGAC TGCCAGAGAC CTCAGCATGT TTGCCCCAAA CATGACACAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GCAATGACTG ATGACATTAT TGTAGAAGCT 
TGCCAAGGCC CATCTGTGGC CGATGTGGTA TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAAGAGAACC TAGATCATCT TAAAGCATTC CTGGGGGAAA GTGTCTCTGC CCTTGACATA 
AAGGAAAACT GCATGAGGGT TGGCCTGGTG GCCTATAGCA ACGAAACAAG GGTGATCAGT 
TCTCTGAGCA TGGGTGTCAA CAAGACGGAG GTCTTACAGC GCATACAGGA TCTGTCCCCT 
CACGTAGGGC AGGCCTACAC AGGAGCTGCC CTCAGAAAGA CGAGGAAGGA AGTCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTGCCTCAGA TTGCTGTGCT GGTGACCCAC 
AGAGCTTCAG ATGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
GTCTTTACCA TGGGCGTAGA GGGGGCTAAC CCAGAACAGC TGGAGAAGAT CGCATCCTAC 
CCTGCCGAGC AGTTCACCTC CAAACTGAGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAGCTGAG GAACCAAATC ACACACACAG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCAGC CTGTGTGGAC ACAGAGGAAG CGGATATCTA TCTGCTCATT 
GATGGTTCAG GGAACACGCA GCCCACAGAC TTCCATGAGA TGAAGATCTT CCTGTCCGAG 
GTGGTGGACA TGTTCAACAT CGCTCCCCAC AAGGTGCGAG TCGGGGCCGT GCAGTACGCC 
GACACCTGGG ACCTGGAATT TGAGATCTCT AAGTATACTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGACA ACATCAGGCA GATGGGTGGA AACACCAATA CGGGTGCAGC CCTGAACTTC 
ACCCTGACGC TGTTGCAAAG AGCAAAGAAG CAACGGGGAA ACAAAGTGCC ATGTCACCTG 
GTTGTCCTGA CAAACGGCAT GTCCCAGGAC AGCGTCCTGG GTCCCGCGCA TAAGCTGAGG 
GAGGAAAACA TCCGTGTGCA TGCGATCGGC GTCAAGGAAG CCAACCAAAC ACAGCTGCGG 
GAGATAGCGG GAGATGAAAA GCGAGTTTAT TACGTGCATG AATTCGATGC CTTGAGGGAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA TATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATTGGAC TCGAAAACTT CAGCAAGATG 
AAGGTGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGTGTGGTCC AGTTCAGCCA CGAAAACAGG GAGGAGTTTC AGCTCAACAC ATTCATGTCT 
CAAAATGACA TTGCCAACGC CATCGACCAA ATGGCTCACA TTGGAGAGAC AACCTTGACA 
GGCAGTGCGC TGACCTTTGT GTCTCAGTAC TTCAGTCCTG AAAAGGGGGC CCGGCCCAAT 
GTTAGGAAGT TCCTCATTCT CATCACGGAT GGCGAGGCTC AGGACATAGT AAAGGACCCA 
GCAGTAGCCC TTCGAAAAGA CGGTGTGATT ATCTATTCTG TGGGAGTGTT CGGCTCCAAT 
GTCACCCAGC TCGAGGAGAT CAGTGGGAAG CCGGAGATGG TTTTCTATGT TGAGAATTTT 
GACATCCTAC AGCATATTGA AGATGATCTC GTTCTGGGAA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTAGTG TTTGTCATCG ACAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCTCTG AAATATGCTG ATGACCCAGA GGTGCTGTTT 
TACCTGGATG AACTGGGCAC AAAGCTGGAG GTGATTTCAG TGCTCCAGAA TGACCAGCCC 
ATGGGTGGTA ACACTTACAC TGCTGAGGCC CTGGCTTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACTGATGGA 
GAATCTCATG ATGCAGAGAA GCTCAATGCC ACCGCCAAGG CCCTGAGGGA CAAAGGCATT 
CTGGTCCTAG CTGTGGGGAT TGCTGGCGCC AATACCTGGG AGCTCTTGGC CATGGCTGGG 
TCGAGTGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGGAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTCGATTGCG AAATTGAAAA AGTTGACCTC 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGAG 
TTTTTGGCAT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGAGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACATTCAC AGGGGAAAAG 
GAGATATCCA CACAGATTGA GGCCATCCAG CAGATCTTTG GTTACACCCA CATTGGAGAT 
GCACTCAGGA AGGTGAAGCA TTACTTTCGG CCAGACACGG GCAGCAGGAT CAACGCCGGT 
ACCCCTCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGATGAGGT GGCCCAGGCG 
GCTGAGGAGC TGAGACACAA GGGTGTGGAC ATCTACTCGG TGGGCATCGG GGACGTGGAT 
GACCAGCAGC TGATCCAGAT AACCGGGACA GCGGAGAAAA AACTGACGGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGTAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGTT TCGTGGATGT GGTGGTTGGA TTTGATATCT CAAGTCAGCG GAGAGGGCAG 
ACTTTGCTCG AAGGTCAGCC TTGGATGGGG ACCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAGGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ATGCTATGGA GAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTGAGCAGCC TGCAGGGTGT GACAGTGAAC GGCCCATCTC GCCTCAACCC AAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAACAAG TCCGCTGCTC GAGGGAAGGT GGTCCTTCTG 
TTTTCAGATG GATTGGATGA CGGCGTTGAA AAACTTGAAC AGAAATCTGA TGAACTCAGA 
AAGGAAGGCC TGAATGCCCT CATAACCATC GCTGTGGACG GAGCTGCCGA TTCCAGTGAC 
CTGGCTGACC TTCTCTACAT TGAATTTGGG AAAGGATTTG AGTACAGAAC ACAGTTCACA 
ATCGGAATGG GGAACCTTGG GAGTCACCTG TCAAAGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTTTGCTCTG CAAGTGCACA GGATGGGACG GCGCCATGGG GGATCCGGGC 
TCAGCAGGGG AAAAGGGACC CCCAGGATTT AAAGGCAGTG AAGGCTACCT GGGAGAAGAA 
GGCATTGCTG GAGAAAGAGG AGCCTTGGGA CCAATGGGAG AGCAAGGTAC AAAGGGATGC 
TTAGGTGCCA AAGGGCCCAA GGGAGCCCGA GGACTCAGCG GAGAAGAGGG TGAAGTTGGG 
GAAGATGGGC TTGACGGATT AAATGGAGAA CAGGGTGACA GTGGGATTCC TGGAAGAAGA 
GGAGAAAGGG GTGATGCGGG ATCTCAGGGA AGCCCAGGCA AGAGAGGGGC TTCTGGTGAC 
CGTGGACCGA AGGGCCTACG AGGAGATCCG GGAACTCCTG GACGTGATAA TAGCATACAG 
GGACCCAAGG GCTTGAAAGG AGACCCTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGGACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACATACAGGC 
CCACAGGGAA ATCCAGGCAT CACAGGCCCA GATGGACTTG AAGGTTCGCC CGGACTTAAG 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAGG CTTCGGAATG 
AAAGGTCCCC AGGGGCCTCC AGGACCCAAA GGACGAGCTG GGAACCAAGG CCATTGGGGA 
AGCCAAGGAA GCAAAGGAGA ACCTGGAGAC CTGGGGGAAA AGGGAGCTGC TGGCTTTCCA 
GGGCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCAGGAT ATGGTAGCAT TGGCCGAAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC CAAAGGGTGA TATTGGGGAC 
CCTGGCGATC CAGGAGAGGC TGGTCCCAAG GGAGCCAGAG GCAAAACGGT ATCTGCTGGG 
ATTCCAGGAG AGCCAGGATC CCCCGGGGAG CCAGGACCCC CTGGAAGGAA GGGCATGAAA 
GGAGCCCGAG GACTGGCTTC ATTTTCCACA TGTGACCTCA TTCAGTATGT GCGGGAACAC 
AGTCCTGTCA GACACGGAAA ACCTGAGTGC CCAGTGCATC CAACTGAGTT GGTGTTTGTC 
CTGGACCAGT CCCGGGATGT CACAGAACAG GACTTTGAGC GGATGAAGGG GATGATGGCC 
TCCTTAGTTA GGGACATCAA AGTTAGGGAT GCCAGCTGCC CCGTGGGTGC CCGCGTTGCC 
ATCCTAGCCT ACAACTCCCA CACCAGGCAC CTCATCCGCT TCTCGGATGC CTACAGGAAG 
GACCAGCTGC TCAGGGAAAT TAAAGCTCTT CCTTACGAGA AATCCTCCGA CAGCAGGGAG 
ATTGGCAAAG CCATGAGGTT TATCTCCAGG AATGTCTTCA AGAGAACCCT CCCAGGGGCT 
CACGTGAGAA GAATCGCCAC GTTCTTTAGC AGTGGCCAGT CAGCCGATGT CCAGTCCATT 
ACCACAGCTG CCATGGAGTT CAGTGCCCTG GACATCATAC CAGTGGTGAT TGCATTCAGC 
AACGTGCCCT CGGTCAAGCG TGCCTTTTCG ATTGATGACA CTGGTACATT CCAAGTCCTT 
GTGGTTCCCT CCAGGCCTGA CCAAGTGCCA GCGTTAGAGA GACTCCAGCG GTGCACTTTC 
TGCTATGACC TATGCAAGCC AGATGCTTCT TGTGACCAAG CCAAACCCCC CATTCAGTCC 
TACCTGGATG CTGCATTCCT GCTGGATAGC TCCTGGCATG TGGGAGGTGC AGAATTTGAA 
GACATGAGAG ACCTCCTGGA GGCCCTGCTA GATCACTTTG AAATCGCTTC AGAGCCAGAG 
ACGTCTGTCA CTGGAGACAG GGTGGCCCTA CTGAGTCATG CTCCCGTTGA CTTCCTACCC 
AACACCCAGA GGAGTCCCGT TAGAACTGAG TTCAACCTAA CCACCTATAG CAGTAAGCAC 
CTCATGAAGA GACACGTGGA GCAAGCAGTC CAGCAGCTGA ATGGAGATGC TTTCCTTGGT 
CATGCCCTCA GGTGGGCACT GGACAATGTC TTTCTAAACA CGCCCAATCT GAGAAGGAAC 
AAAGTCATAT TTGTGATATC TGCTGGGGAA ACCAGCCACC TGGATGCGGA AACCTTAAAG 
AAAGAGTCCC TGCGAGCCAA ATGTCATGGG TATGCCCTCT TTGTGTTTTC CTTGGGCCCT 
GACTGGGATG ACAAAGAACT GGAAGACCTG GCCAGCCACC CACTTGACCA GCACTTGATC 
CAGCTTGGCC GAATTCATAA ACCTGACCAC GGATATGGTG TAAAGTTTGT GAAGTCCTTT 
ATAAACTCCA TCAGGCATGG GATAAACAAG TATCCACCGG TCAACTTTAA AGCCAAGTGC 
AGCAGACTCA GCTCGGCGGC TCCGAAGCTG TCCCCACGCC AGTTTCGAAG CTTTGTTCCG 
GGGCCACAGA AAGCTCTCCT CAAAGACCAC ACACTGGAGG CAGCAAAGCT CTTTCAAGAT 
AAAAAACGCC TTTCAAGTAT CCTGAGAAGT GGCAGGGCTG CTCTTTCAAG TCATTCCAGA 
AGCACACGCT ACTCCTTTAA ACAGGGGAGA GAAGCGACAA AAGCTACTTC TAAACTCGGT 
AAACGAAGTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a6 ( XM_008757863.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008756085.1 Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X5, mRNA.

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CloneID	ORa18100
Clone ID Related Accession (Same CDS sequence)	XM_008757862.2 , XM_002727098.5 , XM_008757863.2 , XM_006226549.3
Accession Version	XM_008757863.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-6(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008757863.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGA TTTTGTTCCT GATGATGATT TGTTTACACG TTTGTGCGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGATGT GGTTTTTCTG GTGGACAGTT CCGATCACCT AGGGATGAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAA AAGCTAGTCA GCAGCCTCCC CGTAGAGGCC 
AACAAGTACC GTGTGGCCCT GGCCCAGTAC AGTGATGCTC TCCACAATGA GTTCCACCTA 
GGCGCCTTCA AGAACAGGAA CCCCATGCTG AACCACCTCA AGAAGAACTT CGGGTTCATC 
GGCGGCTCCC TGAAGATTGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAACG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCG 
GAGTCCGAGG ATGACGTAGA AGAGGCTTCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAGACCTGA AGGCCATGGC CACCTCTCAG 
TTTCATTTCA ACCTCAGGAC TGCCAGAGAC CTCAGCATGT TTGCCCCAAA CATGACACAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GCAATGACTG ATGACATTAT TGTAGAAGCT 
TGCCAAGGCC CATCTGTGGC CGATGTGGTA TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAAGAGAACC TAGATCATCT TAAAGCATTC CTGGGGGAAA GTGTCTCTGC CCTTGACATA 
AAGGAAAACT GCATGAGGGT TGGCCTGGTG GCCTATAGCA ACGAAACAAG GGTGATCAGT 
TCTCTGAGCA TGGGTGTCAA CAAGACGGAG GTCTTACAGC GCATACAGGA TCTGTCCCCT 
CACGTAGGGC AGGCCTACAC AGGAGCTGCC CTCAGAAAGA CGAGGAAGGA AGTCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTGCCTCAGA TTGCTGTGCT GGTGACCCAC 
AGAGCTTCAG ATGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
GTCTTTACCA TGGGCGTAGA GGGGGCTAAC CCAGAACAGC TGGAGAAGAT CGCATCCTAC 
CCTGCCGAGC AGTTCACCTC CAAACTGAGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAGCTGAG GAACCAAATC ACACACACAG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCAGC CTGTGTGGAC ACAGAGGAAG CGGATATCTA TCTGCTCATT 
GATGGTTCAG GGAACACGCA GCCCACAGAC TTCCATGAGA TGAAGATCTT CCTGTCCGAG 
GTGGTGGACA TGTTCAACAT CGCTCCCCAC AAGGTGCGAG TCGGGGCCGT GCAGTACGCC 
GACACCTGGG ACCTGGAATT TGAGATCTCT AAGTATACTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGACA ACATCAGGCA GATGGGTGGA AACACCAATA CGGGTGCAGC CCTGAACTTC 
ACCCTGACGC TGTTGCAAAG AGCAAAGAAG CAACGGGGAA ACAAAGTGCC ATGTCACCTG 
GTTGTCCTGA CAAACGGCAT GTCCCAGGAC AGCGTCCTGG GTCCCGCGCA TAAGCTGAGG 
GAGGAAAACA TCCGTGTGCA TGCGATCGGC GTCAAGGAAG CCAACCAAAC ACAGCTGCGG 
GAGATAGCGG GAGATGAAAA GCGAGTTTAT TACGTGCATG AATTCGATGC CTTGAGGGAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA TATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATTGGAC TCGAAAACTT CAGCAAGATG 
AAGGTGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGTGTGGTCC AGTTCAGCCA CGAAAACAGG GAGGAGTTTC AGCTCAACAC ATTCATGTCT 
CAAAATGACA TTGCCAACGC CATCGACCAA ATGGCTCACA TTGGAGAGAC AACCTTGACA 
GGCAGTGCGC TGACCTTTGT GTCTCAGTAC TTCAGTCCTG AAAAGGGGGC CCGGCCCAAT 
GTTAGGAAGT TCCTCATTCT CATCACGGAT GGCGAGGCTC AGGACATAGT AAAGGACCCA 
GCAGTAGCCC TTCGAAAAGA CGGTGTGATT ATCTATTCTG TGGGAGTGTT CGGCTCCAAT 
GTCACCCAGC TCGAGGAGAT CAGTGGGAAG CCGGAGATGG TTTTCTATGT TGAGAATTTT 
GACATCCTAC AGCATATTGA AGATGATCTC GTTCTGGGAA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTAGTG TTTGTCATCG ACAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCTCTG AAATATGCTG ATGACCCAGA GGTGCTGTTT 
TACCTGGATG AACTGGGCAC AAAGCTGGAG GTGATTTCAG TGCTCCAGAA TGACCAGCCC 
ATGGGTGGTA ACACTTACAC TGCTGAGGCC CTGGCTTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACTGATGGA 
GAATCTCATG ATGCAGAGAA GCTCAATGCC ACCGCCAAGG CCCTGAGGGA CAAAGGCATT 
CTGGTCCTAG CTGTGGGGAT TGCTGGCGCC AATACCTGGG AGCTCTTGGC CATGGCTGGG 
TCGAGTGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGGAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTCGATTGCG AAATTGAAAA AGTTGACCTC 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGAG 
TTTTTGGCAT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGAGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACATTCAC AGGGGAAAAG 
GAGATATCCA CACAGATTGA GGCCATCCAG CAGATCTTTG GTTACACCCA CATTGGAGAT 
GCACTCAGGA AGGTGAAGCA TTACTTTCGG CCAGACACGG GCAGCAGGAT CAACGCCGGT 
ACCCCTCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGATGAGGT GGCCCAGGCG 
GCTGAGGAGC TGAGACACAA GGGTGTGGAC ATCTACTCGG TGGGCATCGG GGACGTGGAT 
GACCAGCAGC TGATCCAGAT AACCGGGACA GCGGAGAAAA AACTGACGGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGTAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGTT TCGTGGATGT GGTGGTTGGA TTTGATATCT CAAGTCAGCG GAGAGGGCAG 
ACTTTGCTCG AAGGTCAGCC TTGGATGGGG ACCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAGGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ATGCTATGGA GAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTGAGCAGCC TGCAGGGTGT GACAGTGAAC GGCCCATCTC GCCTCAACCC AAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAACAAG TCCGCTGCTC GAGGGAAGGT GGTCCTTCTG 
TTTTCAGATG GATTGGATGA CGGCGTTGAA AAACTTGAAC AGAAATCTGA TGAACTCAGA 
AAGGAAGGCC TGAATGCCCT CATAACCATC GCTGTGGACG GAGCTGCCGA TTCCAGTGAC 
CTGGCTGACC TTCTCTACAT TGAATTTGGG AAAGGATTTG AGTACAGAAC ACAGTTCACA 
ATCGGAATGG GGAACCTTGG GAGTCACCTG TCAAAGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTTTGCTCTG CAAGTGCACA GGATGGGACG GCGCCATGGG GGATCCGGGC 
TCAGCAGGGG AAAAGGGACC CCCAGGATTT AAAGGCAGTG AAGGCTACCT GGGAGAAGAA 
GGCATTGCTG GAGAAAGAGG AGCCTTGGGA CCAATGGGAG AGCAAGGTAC AAAGGGATGC 
TTAGGTGCCA AAGGGCCCAA GGGAGCCCGA GGACTCAGCG GAGAAGAGGG TGAAGTTGGG 
GAAGATGGGC TTGACGGATT AAATGGAGAA CAGGGTGACA GTGGGATTCC TGGAAGAAGA 
GGAGAAAGGG GTGATGCGGG ATCTCAGGGA AGCCCAGGCA AGAGAGGGGC TTCTGGTGAC 
CGTGGACCGA AGGGCCTACG AGGAGATCCG GGAACTCCTG GACGTGATAA TAGCATACAG 
GGACCCAAGG GCTTGAAAGG AGACCCTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGGACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACATACAGGC 
CCACAGGGAA ATCCAGGCAT CACAGGCCCA GATGGACTTG AAGGTTCGCC CGGACTTAAG 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAGG CTTCGGAATG 
AAAGGTCCCC AGGGGCCTCC AGGACCCAAA GGACGAGCTG GGAACCAAGG CCATTGGGGA 
AGCCAAGGAA GCAAAGGAGA ACCTGGAGAC CTGGGGGAAA AGGGAGCTGC TGGCTTTCCA 
GGGCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCAGGAT ATGGTAGCAT TGGCCGAAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC CAAAGGGTGA TATTGGGGAC 
CCTGGCGATC CAGGAGAGGC TGGTCCCAAG GGAGCCAGAG GCAAAACGGT ATCTGCTGGG 
ATTCCAGGAG AGCCAGGATC CCCCGGGGAG CCAGGACCCC CTGGAAGGAA GGGCATGAAA 
GGAGCCCGAG GACTGGCTTC ATTTTCCACA TGTGACCTCA TTCAGTATGT GCGGGAACAC 
AGTCCTGTCA GACACGGAAA ACCTGAGTGC CCAGTGCATC CAACTGAGTT GGTGTTTGTC 
CTGGACCAGT CCCGGGATGT CACAGAACAG GACTTTGAGC GGATGAAGGG GATGATGGCC 
TCCTTAGTTA GGGACATCAA AGTTAGGGAT GCCAGCTGCC CCGTGGGTGC CCGCGTTGCC 
ATCCTAGCCT ACAACTCCCA CACCAGGCAC CTCATCCGCT TCTCGGATGC CTACAGGAAG 
GACCAGCTGC TCAGGGAAAT TAAAGCTCTT CCTTACGAGA AATCCTCCGA CAGCAGGGAG 
ATTGGCAAAG CCATGAGGTT TATCTCCAGG AATGTCTTCA AGAGAACCCT CCCAGGGGCT 
CACGTGAGAA GAATCGCCAC GTTCTTTAGC AGTGGCCAGT CAGCCGATGT CCAGTCCATT 
ACCACAGCTG CCATGGAGTT CAGTGCCCTG GACATCATAC CAGTGGTGAT TGCATTCAGC 
AACGTGCCCT CGGTCAAGCG TGCCTTTTCG ATTGATGACA CTGGTACATT CCAAGTCCTT 
GTGGTTCCCT CCAGGCCTGA CCAAGTGCCA GCGTTAGAGA GACTCCAGCG GTGCACTTTC 
TGCTATGACC TATGCAAGCC AGATGCTTCT TGTGACCAAG CCAAACCCCC CATTCAGTCC 
TACCTGGATG CTGCATTCCT GCTGGATAGC TCCTGGCATG TGGGAGGTGC AGAATTTGAA 
GACATGAGAG ACCTCCTGGA GGCCCTGCTA GATCACTTTG AAATCGCTTC AGAGCCAGAG 
ACGTCTGTCA CTGGAGACAG GGTGGCCCTA CTGAGTCATG CTCCCGTTGA CTTCCTACCC 
AACACCCAGA GGAGTCCCGT TAGAACTGAG TTCAACCTAA CCACCTATAG CAGTAAGCAC 
CTCATGAAGA GACACGTGGA GCAAGCAGTC CAGCAGCTGA ATGGAGATGC TTTCCTTGGT 
CATGCCCTCA GGTGGGCACT GGACAATGTC TTTCTAAACA CGCCCAATCT GAGAAGGAAC 
AAAGTCATAT TTGTGATATC TGCTGGGGAA ACCAGCCACC TGGATGCGGA AACCTTAAAG 
AAAGAGTCCC TGCGAGCCAA ATGTCATGGG TATGCCCTCT TTGTGTTTTC CTTGGGCCCT 
GACTGGGATG ACAAAGAACT GGAAGACCTG GCCAGCCACC CACTTGACCA GCACTTGATC 
CAGCTTGGCC GAATTCATAA ACCTGACCAC GGATATGGTG TAAAGTTTGT GAAGTCCTTT 
ATAAACTCCA TCAGGCATGG GATAAACAAG TATCCACCGG TCAACTTTAA AGCCAAGTGC 
AGCAGACTCA GCTCGGCGGC TCCGAAGCTG TCCCCACGCC AGTTTCGAAG CTTTGTTCCG 
GGGCCACAGA AAGCTCTCCT CAAAGACCAC ACACTGGAGG CAGCAAAGCT CTTTCAAGAT 
AAAAAACGCC TTTCAAGTAT CCTGAGAAGT GGCAGGGCTG CTCTTTCAAG TCATTCCAGA 
AGCACACGCT ACTCCTTTAA ACAGGGGAGA GAAGCGACAA AAGCTACTTC TAAACTCGGT 
AAACGAAGTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008756085.1
CDS	824..7618
Translation	MLLILFLMMICLHVCANQDSGPEYADVVFLVDSSDHLGMKSFPLVKTFIQKLVSSLPVEANKYRVALAQYSDALHNEFHLGAFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEASKALREDGVKIISVGVQKASEEDLKAMATSQFHFNLRTARDLSMFAPNMTQIIKDVTQYREAMTDDIIVEACQGPSVADVVFLLDMAINGSQENLDHLKAFLGESVSALDIKENCMRVGLVAYSNETRVISSLSMGVNKTEVLQRIQDLSPHVGQAYTGAALRKTRKEVFSAQRGSRKNQGVPQIAVLVTHRASDDNVTKAAVNLRREGVTVFTMGVEGANPEQLEKIASYPAEQFTSKLSNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGNTQPTDFHEMKIFLSEVVDMFNIAPHKVRVGAVQYADTWDLEFEISKYTNKPDLGKAIDNIRQMGGNTNTGAALNFTLTLLQRAKKQRGNKVPCHLVVLTNGMSQDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGDEKRVYYVHEFDALRDIRNQVVQEICAEEACRDMKADIMFLVDSSGSIGLENFSKMKVFMKNLVSKSQIGADRVQIGVVQFSHENREEFQLNTFMSQNDIANAIDQMAHIGETTLTGSALTFVSQYFSPEKGARPNVRKFLILITDGEAQDIVKDPAVALRKDGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVISVLQNDQPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNATAKALRDKGILVLAVGIAGANTWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFQKMKEFLASVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEKEISTQIEAIQQIFGYTHIGDALRKVKHYFRPDTGSRINAGTPQVLLVLTDGRSQDEVAQAAEELRHKGVDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVKKRIVRNICTSGGESNCFVDVVVGFDISSQRRGQTLLEGQPWMGTYLQDLLRAISSLNGVSCEVGTETQVSIAFQVTNAMERYPSKFEIYSENILSSLQGVTVNGPSRLNPNLLSSLWDTFQNKSAARGKVVLLFSDGLDDGVEKLEQKSDELRKEGLNALITIAVDGAADSSDLADLLYIEFGKGFEYRTQFTIGMGNLGSHLSKQLINVAERTCCCLLCKCTGWDGAMGDPGSAGEKGPPGFKGSEGYLGEEGIAGERGALGPMGEQGTKGCLGAKGPKGARGLSGEEGEVGEDGLDGLNGEQGDSGIPGRRGERGDAGSQGSPGKRGASGDRGPKGLRGDPGTPGRDNSIQGPKGLKGDPGRQGRRGWPGSPGTPGSRRKMVVHGRRGHTGPQGNPGITGPDGLEGSPGLKGPQGPRGEVGEKGEKGGFGMKGPQGPPGPKGRAGNQGHWGSQGSKGEPGDLGEKGAAGFPGPRGLQGDDGSPGYGSIGRKGTKGQEGFPGESGPKGDIGDPGDPGEAGPKGARGKTVSAGIPGEPGSPGEPGPPGRKGMKGARGLASFSTCDLIQYVREHSPVRHGKPECPVHPTELVFVLDQSRDVTEQDFERMKGMMASLVRDIKVRDASCPVGARVAILAYNSHTRHLIRFSDAYRKDQLLREIKALPYEKSSDSREIGKAMRFISRNVFKRTLPGAHVRRIATFFSSGQSADVQSITTAAMEFSALDIIPVVIAFSNVPSVKRAFSIDDTGTFQVLVVPSRPDQVPALERLQRCTFCYDLCKPDASCDQAKPPIQSYLDAAFLLDSSWHVGGAEFEDMRDLLEALLDHFEIASEPETSVTGDRVALLSHAPVDFLPNTQRSPVRTEFNLTTYSSKHLMKRHVEQAVQQLNGDAFLGHALRWALDNVFLNTPNLRRNKVIFVISAGETSHLDAETLKKESLRAKCHGYALFVFSLGPDWDDKELEDLASHPLDQHLIQLGRIHKPDHGYGVKFVKSFINSIRHGINKYPPVNFKAKCSRLSSAAPKLSPRQFRSFVPGPQKALLKDHTLEAAKLFQDKKRLSSILRSGRAALSSHSRSTRYSFKQGREATKATSKLGKRSD

Target ORF information:

RefSeq Version	XM_008757863.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008757863.2

ORF Insert Sequence:

ATGCTGCTGA TTTTGTTCCT GATGATGATT TGTTTACACG TTTGTGCGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGATGT GGTTTTTCTG GTGGACAGTT CCGATCACCT AGGGATGAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAA AAGCTAGTCA GCAGCCTCCC CGTAGAGGCC 
AACAAGTACC GTGTGGCCCT GGCCCAGTAC AGTGATGCTC TCCACAATGA GTTCCACCTA 
GGCGCCTTCA AGAACAGGAA CCCCATGCTG AACCACCTCA AGAAGAACTT CGGGTTCATC 
GGCGGCTCCC TGAAGATTGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAACG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCG 
GAGTCCGAGG ATGACGTAGA AGAGGCTTCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAGACCTGA AGGCCATGGC CACCTCTCAG 
TTTCATTTCA ACCTCAGGAC TGCCAGAGAC CTCAGCATGT TTGCCCCAAA CATGACACAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GCAATGACTG ATGACATTAT TGTAGAAGCT 
TGCCAAGGCC CATCTGTGGC CGATGTGGTA TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAAGAGAACC TAGATCATCT TAAAGCATTC CTGGGGGAAA GTGTCTCTGC CCTTGACATA 
AAGGAAAACT GCATGAGGGT TGGCCTGGTG GCCTATAGCA ACGAAACAAG GGTGATCAGT 
TCTCTGAGCA TGGGTGTCAA CAAGACGGAG GTCTTACAGC GCATACAGGA TCTGTCCCCT 
CACGTAGGGC AGGCCTACAC AGGAGCTGCC CTCAGAAAGA CGAGGAAGGA AGTCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTGCCTCAGA TTGCTGTGCT GGTGACCCAC 
AGAGCTTCAG ATGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
GTCTTTACCA TGGGCGTAGA GGGGGCTAAC CCAGAACAGC TGGAGAAGAT CGCATCCTAC 
CCTGCCGAGC AGTTCACCTC CAAACTGAGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAGCTGAG GAACCAAATC ACACACACAG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCAGC CTGTGTGGAC ACAGAGGAAG CGGATATCTA TCTGCTCATT 
GATGGTTCAG GGAACACGCA GCCCACAGAC TTCCATGAGA TGAAGATCTT CCTGTCCGAG 
GTGGTGGACA TGTTCAACAT CGCTCCCCAC AAGGTGCGAG TCGGGGCCGT GCAGTACGCC 
GACACCTGGG ACCTGGAATT TGAGATCTCT AAGTATACTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGACA ACATCAGGCA GATGGGTGGA AACACCAATA CGGGTGCAGC CCTGAACTTC 
ACCCTGACGC TGTTGCAAAG AGCAAAGAAG CAACGGGGAA ACAAAGTGCC ATGTCACCTG 
GTTGTCCTGA CAAACGGCAT GTCCCAGGAC AGCGTCCTGG GTCCCGCGCA TAAGCTGAGG 
GAGGAAAACA TCCGTGTGCA TGCGATCGGC GTCAAGGAAG CCAACCAAAC ACAGCTGCGG 
GAGATAGCGG GAGATGAAAA GCGAGTTTAT TACGTGCATG AATTCGATGC CTTGAGGGAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA TATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATTGGAC TCGAAAACTT CAGCAAGATG 
AAGGTGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGTGTGGTCC AGTTCAGCCA CGAAAACAGG GAGGAGTTTC AGCTCAACAC ATTCATGTCT 
CAAAATGACA TTGCCAACGC CATCGACCAA ATGGCTCACA TTGGAGAGAC AACCTTGACA 
GGCAGTGCGC TGACCTTTGT GTCTCAGTAC TTCAGTCCTG AAAAGGGGGC CCGGCCCAAT 
GTTAGGAAGT TCCTCATTCT CATCACGGAT GGCGAGGCTC AGGACATAGT AAAGGACCCA 
GCAGTAGCCC TTCGAAAAGA CGGTGTGATT ATCTATTCTG TGGGAGTGTT CGGCTCCAAT 
GTCACCCAGC TCGAGGAGAT CAGTGGGAAG CCGGAGATGG TTTTCTATGT TGAGAATTTT 
GACATCCTAC AGCATATTGA AGATGATCTC GTTCTGGGAA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTAGTG TTTGTCATCG ACAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCTCTG AAATATGCTG ATGACCCAGA GGTGCTGTTT 
TACCTGGATG AACTGGGCAC AAAGCTGGAG GTGATTTCAG TGCTCCAGAA TGACCAGCCC 
ATGGGTGGTA ACACTTACAC TGCTGAGGCC CTGGCTTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACTGATGGA 
GAATCTCATG ATGCAGAGAA GCTCAATGCC ACCGCCAAGG CCCTGAGGGA CAAAGGCATT 
CTGGTCCTAG CTGTGGGGAT TGCTGGCGCC AATACCTGGG AGCTCTTGGC CATGGCTGGG 
TCGAGTGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGGAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTCGATTGCG AAATTGAAAA AGTTGACCTC 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGAG 
TTTTTGGCAT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGAGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACATTCAC AGGGGAAAAG 
GAGATATCCA CACAGATTGA GGCCATCCAG CAGATCTTTG GTTACACCCA CATTGGAGAT 
GCACTCAGGA AGGTGAAGCA TTACTTTCGG CCAGACACGG GCAGCAGGAT CAACGCCGGT 
ACCCCTCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGATGAGGT GGCCCAGGCG 
GCTGAGGAGC TGAGACACAA GGGTGTGGAC ATCTACTCGG TGGGCATCGG GGACGTGGAT 
GACCAGCAGC TGATCCAGAT AACCGGGACA GCGGAGAAAA AACTGACGGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGTAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGTT TCGTGGATGT GGTGGTTGGA TTTGATATCT CAAGTCAGCG GAGAGGGCAG 
ACTTTGCTCG AAGGTCAGCC TTGGATGGGG ACCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAGGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ATGCTATGGA GAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTGAGCAGCC TGCAGGGTGT GACAGTGAAC GGCCCATCTC GCCTCAACCC AAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAACAAG TCCGCTGCTC GAGGGAAGGT GGTCCTTCTG 
TTTTCAGATG GATTGGATGA CGGCGTTGAA AAACTTGAAC AGAAATCTGA TGAACTCAGA 
AAGGAAGGCC TGAATGCCCT CATAACCATC GCTGTGGACG GAGCTGCCGA TTCCAGTGAC 
CTGGCTGACC TTCTCTACAT TGAATTTGGG AAAGGATTTG AGTACAGAAC ACAGTTCACA 
ATCGGAATGG GGAACCTTGG GAGTCACCTG TCAAAGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTTTGCTCTG CAAGTGCACA GGATGGGACG GCGCCATGGG GGATCCGGGC 
TCAGCAGGGG AAAAGGGACC CCCAGGATTT AAAGGCAGTG AAGGCTACCT GGGAGAAGAA 
GGCATTGCTG GAGAAAGAGG AGCCTTGGGA CCAATGGGAG AGCAAGGTAC AAAGGGATGC 
TTAGGTGCCA AAGGGCCCAA GGGAGCCCGA GGACTCAGCG GAGAAGAGGG TGAAGTTGGG 
GAAGATGGGC TTGACGGATT AAATGGAGAA CAGGGTGACA GTGGGATTCC TGGAAGAAGA 
GGAGAAAGGG GTGATGCGGG ATCTCAGGGA AGCCCAGGCA AGAGAGGGGC TTCTGGTGAC 
CGTGGACCGA AGGGCCTACG AGGAGATCCG GGAACTCCTG GACGTGATAA TAGCATACAG 
GGACCCAAGG GCTTGAAAGG AGACCCTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGGACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACATACAGGC 
CCACAGGGAA ATCCAGGCAT CACAGGCCCA GATGGACTTG AAGGTTCGCC CGGACTTAAG 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAGG CTTCGGAATG 
AAAGGTCCCC AGGGGCCTCC AGGACCCAAA GGACGAGCTG GGAACCAAGG CCATTGGGGA 
AGCCAAGGAA GCAAAGGAGA ACCTGGAGAC CTGGGGGAAA AGGGAGCTGC TGGCTTTCCA 
GGGCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCAGGAT ATGGTAGCAT TGGCCGAAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC CAAAGGGTGA TATTGGGGAC 
CCTGGCGATC CAGGAGAGGC TGGTCCCAAG GGAGCCAGAG GCAAAACGGT ATCTGCTGGG 
ATTCCAGGAG AGCCAGGATC CCCCGGGGAG CCAGGACCCC CTGGAAGGAA GGGCATGAAA 
GGAGCCCGAG GACTGGCTTC ATTTTCCACA TGTGACCTCA TTCAGTATGT GCGGGAACAC 
AGTCCTGTCA GACACGGAAA ACCTGAGTGC CCAGTGCATC CAACTGAGTT GGTGTTTGTC 
CTGGACCAGT CCCGGGATGT CACAGAACAG GACTTTGAGC GGATGAAGGG GATGATGGCC 
TCCTTAGTTA GGGACATCAA AGTTAGGGAT GCCAGCTGCC CCGTGGGTGC CCGCGTTGCC 
ATCCTAGCCT ACAACTCCCA CACCAGGCAC CTCATCCGCT TCTCGGATGC CTACAGGAAG 
GACCAGCTGC TCAGGGAAAT TAAAGCTCTT CCTTACGAGA AATCCTCCGA CAGCAGGGAG 
ATTGGCAAAG CCATGAGGTT TATCTCCAGG AATGTCTTCA AGAGAACCCT CCCAGGGGCT 
CACGTGAGAA GAATCGCCAC GTTCTTTAGC AGTGGCCAGT CAGCCGATGT CCAGTCCATT 
ACCACAGCTG CCATGGAGTT CAGTGCCCTG GACATCATAC CAGTGGTGAT TGCATTCAGC 
AACGTGCCCT CGGTCAAGCG TGCCTTTTCG ATTGATGACA CTGGTACATT CCAAGTCCTT 
GTGGTTCCCT CCAGGCCTGA CCAAGTGCCA GCGTTAGAGA GACTCCAGCG GTGCACTTTC 
TGCTATGACC TATGCAAGCC AGATGCTTCT TGTGACCAAG CCAAACCCCC CATTCAGTCC 
TACCTGGATG CTGCATTCCT GCTGGATAGC TCCTGGCATG TGGGAGGTGC AGAATTTGAA 
GACATGAGAG ACCTCCTGGA GGCCCTGCTA GATCACTTTG AAATCGCTTC AGAGCCAGAG 
ACGTCTGTCA CTGGAGACAG GGTGGCCCTA CTGAGTCATG CTCCCGTTGA CTTCCTACCC 
AACACCCAGA GGAGTCCCGT TAGAACTGAG TTCAACCTAA CCACCTATAG CAGTAAGCAC 
CTCATGAAGA GACACGTGGA GCAAGCAGTC CAGCAGCTGA ATGGAGATGC TTTCCTTGGT 
CATGCCCTCA GGTGGGCACT GGACAATGTC TTTCTAAACA CGCCCAATCT GAGAAGGAAC 
AAAGTCATAT TTGTGATATC TGCTGGGGAA ACCAGCCACC TGGATGCGGA AACCTTAAAG 
AAAGAGTCCC TGCGAGCCAA ATGTCATGGG TATGCCCTCT TTGTGTTTTC CTTGGGCCCT 
GACTGGGATG ACAAAGAACT GGAAGACCTG GCCAGCCACC CACTTGACCA GCACTTGATC 
CAGCTTGGCC GAATTCATAA ACCTGACCAC GGATATGGTG TAAAGTTTGT GAAGTCCTTT 
ATAAACTCCA TCAGGCATGG GATAAACAAG TATCCACCGG TCAACTTTAA AGCCAAGTGC 
AGCAGACTCA GCTCGGCGGC TCCGAAGCTG TCCCCACGCC AGTTTCGAAG CTTTGTTCCG 
GGGCCACAGA AAGCTCTCCT CAAAGACCAC ACACTGGAGG CAGCAAAGCT CTTTCAAGAT 
AAAAAACGCC TTTCAAGTAT CCTGAGAAGT GGCAGGGCTG CTCTTTCAAG TCATTCCAGA 
AGCACACGCT ACTCCTTTAA ACAGGGGAGA GAAGCGACAA AAGCTACTTC TAAACTCGGT 
AAACGAAGTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a6 ( XM_006226549.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006226611.1 Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X2, mRNA.

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CloneID	ORa18100
Clone ID Related Accession (Same CDS sequence)	XM_008757862.2 , XM_002727098.5 , XM_008757863.2 , XM_006226549.3
Accession Version	XM_006226549.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-6(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006226549.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGA TTTTGTTCCT GATGATGATT TGTTTACACG TTTGTGCGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGATGT GGTTTTTCTG GTGGACAGTT CCGATCACCT AGGGATGAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAA AAGCTAGTCA GCAGCCTCCC CGTAGAGGCC 
AACAAGTACC GTGTGGCCCT GGCCCAGTAC AGTGATGCTC TCCACAATGA GTTCCACCTA 
GGCGCCTTCA AGAACAGGAA CCCCATGCTG AACCACCTCA AGAAGAACTT CGGGTTCATC 
GGCGGCTCCC TGAAGATTGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAACG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCG 
GAGTCCGAGG ATGACGTAGA AGAGGCTTCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAGACCTGA AGGCCATGGC CACCTCTCAG 
TTTCATTTCA ACCTCAGGAC TGCCAGAGAC CTCAGCATGT TTGCCCCAAA CATGACACAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GCAATGACTG ATGACATTAT TGTAGAAGCT 
TGCCAAGGCC CATCTGTGGC CGATGTGGTA TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAAGAGAACC TAGATCATCT TAAAGCATTC CTGGGGGAAA GTGTCTCTGC CCTTGACATA 
AAGGAAAACT GCATGAGGGT TGGCCTGGTG GCCTATAGCA ACGAAACAAG GGTGATCAGT 
TCTCTGAGCA TGGGTGTCAA CAAGACGGAG GTCTTACAGC GCATACAGGA TCTGTCCCCT 
CACGTAGGGC AGGCCTACAC AGGAGCTGCC CTCAGAAAGA CGAGGAAGGA AGTCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTGCCTCAGA TTGCTGTGCT GGTGACCCAC 
AGAGCTTCAG ATGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
GTCTTTACCA TGGGCGTAGA GGGGGCTAAC CCAGAACAGC TGGAGAAGAT CGCATCCTAC 
CCTGCCGAGC AGTTCACCTC CAAACTGAGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAGCTGAG GAACCAAATC ACACACACAG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCAGC CTGTGTGGAC ACAGAGGAAG CGGATATCTA TCTGCTCATT 
GATGGTTCAG GGAACACGCA GCCCACAGAC TTCCATGAGA TGAAGATCTT CCTGTCCGAG 
GTGGTGGACA TGTTCAACAT CGCTCCCCAC AAGGTGCGAG TCGGGGCCGT GCAGTACGCC 
GACACCTGGG ACCTGGAATT TGAGATCTCT AAGTATACTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGACA ACATCAGGCA GATGGGTGGA AACACCAATA CGGGTGCAGC CCTGAACTTC 
ACCCTGACGC TGTTGCAAAG AGCAAAGAAG CAACGGGGAA ACAAAGTGCC ATGTCACCTG 
GTTGTCCTGA CAAACGGCAT GTCCCAGGAC AGCGTCCTGG GTCCCGCGCA TAAGCTGAGG 
GAGGAAAACA TCCGTGTGCA TGCGATCGGC GTCAAGGAAG CCAACCAAAC ACAGCTGCGG 
GAGATAGCGG GAGATGAAAA GCGAGTTTAT TACGTGCATG AATTCGATGC CTTGAGGGAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA TATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATTGGAC TCGAAAACTT CAGCAAGATG 
AAGGTGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGTGTGGTCC AGTTCAGCCA CGAAAACAGG GAGGAGTTTC AGCTCAACAC ATTCATGTCT 
CAAAATGACA TTGCCAACGC CATCGACCAA ATGGCTCACA TTGGAGAGAC AACCTTGACA 
GGCAGTGCGC TGACCTTTGT GTCTCAGTAC TTCAGTCCTG AAAAGGGGGC CCGGCCCAAT 
GTTAGGAAGT TCCTCATTCT CATCACGGAT GGCGAGGCTC AGGACATAGT AAAGGACCCA 
GCAGTAGCCC TTCGAAAAGA CGGTGTGATT ATCTATTCTG TGGGAGTGTT CGGCTCCAAT 
GTCACCCAGC TCGAGGAGAT CAGTGGGAAG CCGGAGATGG TTTTCTATGT TGAGAATTTT 
GACATCCTAC AGCATATTGA AGATGATCTC GTTCTGGGAA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTAGTG TTTGTCATCG ACAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCTCTG AAATATGCTG ATGACCCAGA GGTGCTGTTT 
TACCTGGATG AACTGGGCAC AAAGCTGGAG GTGATTTCAG TGCTCCAGAA TGACCAGCCC 
ATGGGTGGTA ACACTTACAC TGCTGAGGCC CTGGCTTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACTGATGGA 
GAATCTCATG ATGCAGAGAA GCTCAATGCC ACCGCCAAGG CCCTGAGGGA CAAAGGCATT 
CTGGTCCTAG CTGTGGGGAT TGCTGGCGCC AATACCTGGG AGCTCTTGGC CATGGCTGGG 
TCGAGTGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGGAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTCGATTGCG AAATTGAAAA AGTTGACCTC 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGAG 
TTTTTGGCAT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGAGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACATTCAC AGGGGAAAAG 
GAGATATCCA CACAGATTGA GGCCATCCAG CAGATCTTTG GTTACACCCA CATTGGAGAT 
GCACTCAGGA AGGTGAAGCA TTACTTTCGG CCAGACACGG GCAGCAGGAT CAACGCCGGT 
ACCCCTCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGATGAGGT GGCCCAGGCG 
GCTGAGGAGC TGAGACACAA GGGTGTGGAC ATCTACTCGG TGGGCATCGG GGACGTGGAT 
GACCAGCAGC TGATCCAGAT AACCGGGACA GCGGAGAAAA AACTGACGGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGTAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGTT TCGTGGATGT GGTGGTTGGA TTTGATATCT CAAGTCAGCG GAGAGGGCAG 
ACTTTGCTCG AAGGTCAGCC TTGGATGGGG ACCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAGGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ATGCTATGGA GAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTGAGCAGCC TGCAGGGTGT GACAGTGAAC GGCCCATCTC GCCTCAACCC AAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAACAAG TCCGCTGCTC GAGGGAAGGT GGTCCTTCTG 
TTTTCAGATG GATTGGATGA CGGCGTTGAA AAACTTGAAC AGAAATCTGA TGAACTCAGA 
AAGGAAGGCC TGAATGCCCT CATAACCATC GCTGTGGACG GAGCTGCCGA TTCCAGTGAC 
CTGGCTGACC TTCTCTACAT TGAATTTGGG AAAGGATTTG AGTACAGAAC ACAGTTCACA 
ATCGGAATGG GGAACCTTGG GAGTCACCTG TCAAAGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTTTGCTCTG CAAGTGCACA GGATGGGACG GCGCCATGGG GGATCCGGGC 
TCAGCAGGGG AAAAGGGACC CCCAGGATTT AAAGGCAGTG AAGGCTACCT GGGAGAAGAA 
GGCATTGCTG GAGAAAGAGG AGCCTTGGGA CCAATGGGAG AGCAAGGTAC AAAGGGATGC 
TTAGGTGCCA AAGGGCCCAA GGGAGCCCGA GGACTCAGCG GAGAAGAGGG TGAAGTTGGG 
GAAGATGGGC TTGACGGATT AAATGGAGAA CAGGGTGACA GTGGGATTCC TGGAAGAAGA 
GGAGAAAGGG GTGATGCGGG ATCTCAGGGA AGCCCAGGCA AGAGAGGGGC TTCTGGTGAC 
CGTGGACCGA AGGGCCTACG AGGAGATCCG GGAACTCCTG GACGTGATAA TAGCATACAG 
GGACCCAAGG GCTTGAAAGG AGACCCTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGGACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACATACAGGC 
CCACAGGGAA ATCCAGGCAT CACAGGCCCA GATGGACTTG AAGGTTCGCC CGGACTTAAG 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAGG CTTCGGAATG 
AAAGGTCCCC AGGGGCCTCC AGGACCCAAA GGACGAGCTG GGAACCAAGG CCATTGGGGA 
AGCCAAGGAA GCAAAGGAGA ACCTGGAGAC CTGGGGGAAA AGGGAGCTGC TGGCTTTCCA 
GGGCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCAGGAT ATGGTAGCAT TGGCCGAAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC CAAAGGGTGA TATTGGGGAC 
CCTGGCGATC CAGGAGAGGC TGGTCCCAAG GGAGCCAGAG GCAAAACGGT ATCTGCTGGG 
ATTCCAGGAG AGCCAGGATC CCCCGGGGAG CCAGGACCCC CTGGAAGGAA GGGCATGAAA 
GGAGCCCGAG GACTGGCTTC ATTTTCCACA TGTGACCTCA TTCAGTATGT GCGGGAACAC 
AGTCCTGTCA GACACGGAAA ACCTGAGTGC CCAGTGCATC CAACTGAGTT GGTGTTTGTC 
CTGGACCAGT CCCGGGATGT CACAGAACAG GACTTTGAGC GGATGAAGGG GATGATGGCC 
TCCTTAGTTA GGGACATCAA AGTTAGGGAT GCCAGCTGCC CCGTGGGTGC CCGCGTTGCC 
ATCCTAGCCT ACAACTCCCA CACCAGGCAC CTCATCCGCT TCTCGGATGC CTACAGGAAG 
GACCAGCTGC TCAGGGAAAT TAAAGCTCTT CCTTACGAGA AATCCTCCGA CAGCAGGGAG 
ATTGGCAAAG CCATGAGGTT TATCTCCAGG AATGTCTTCA AGAGAACCCT CCCAGGGGCT 
CACGTGAGAA GAATCGCCAC GTTCTTTAGC AGTGGCCAGT CAGCCGATGT CCAGTCCATT 
ACCACAGCTG CCATGGAGTT CAGTGCCCTG GACATCATAC CAGTGGTGAT TGCATTCAGC 
AACGTGCCCT CGGTCAAGCG TGCCTTTTCG ATTGATGACA CTGGTACATT CCAAGTCCTT 
GTGGTTCCCT CCAGGCCTGA CCAAGTGCCA GCGTTAGAGA GACTCCAGCG GTGCACTTTC 
TGCTATGACC TATGCAAGCC AGATGCTTCT TGTGACCAAG CCAAACCCCC CATTCAGTCC 
TACCTGGATG CTGCATTCCT GCTGGATAGC TCCTGGCATG TGGGAGGTGC AGAATTTGAA 
GACATGAGAG ACCTCCTGGA GGCCCTGCTA GATCACTTTG AAATCGCTTC AGAGCCAGAG 
ACGTCTGTCA CTGGAGACAG GGTGGCCCTA CTGAGTCATG CTCCCGTTGA CTTCCTACCC 
AACACCCAGA GGAGTCCCGT TAGAACTGAG TTCAACCTAA CCACCTATAG CAGTAAGCAC 
CTCATGAAGA GACACGTGGA GCAAGCAGTC CAGCAGCTGA ATGGAGATGC TTTCCTTGGT 
CATGCCCTCA GGTGGGCACT GGACAATGTC TTTCTAAACA CGCCCAATCT GAGAAGGAAC 
AAAGTCATAT TTGTGATATC TGCTGGGGAA ACCAGCCACC TGGATGCGGA AACCTTAAAG 
AAAGAGTCCC TGCGAGCCAA ATGTCATGGG TATGCCCTCT TTGTGTTTTC CTTGGGCCCT 
GACTGGGATG ACAAAGAACT GGAAGACCTG GCCAGCCACC CACTTGACCA GCACTTGATC 
CAGCTTGGCC GAATTCATAA ACCTGACCAC GGATATGGTG TAAAGTTTGT GAAGTCCTTT 
ATAAACTCCA TCAGGCATGG GATAAACAAG TATCCACCGG TCAACTTTAA AGCCAAGTGC 
AGCAGACTCA GCTCGGCGGC TCCGAAGCTG TCCCCACGCC AGTTTCGAAG CTTTGTTCCG 
GGGCCACAGA AAGCTCTCCT CAAAGACCAC ACACTGGAGG CAGCAAAGCT CTTTCAAGAT 
AAAAAACGCC TTTCAAGTAT CCTGAGAAGT GGCAGGGCTG CTCTTTCAAG TCATTCCAGA 
AGCACACGCT ACTCCTTTAA ACAGGGGAGA GAAGCGACAA AAGCTACTTC TAAACTCGGT 
AAACGAAGTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006226611.1
CDS	183..6977
Translation	MLLILFLMMICLHVCANQDSGPEYADVVFLVDSSDHLGMKSFPLVKTFIQKLVSSLPVEANKYRVALAQYSDALHNEFHLGAFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEASKALREDGVKIISVGVQKASEEDLKAMATSQFHFNLRTARDLSMFAPNMTQIIKDVTQYREAMTDDIIVEACQGPSVADVVFLLDMAINGSQENLDHLKAFLGESVSALDIKENCMRVGLVAYSNETRVISSLSMGVNKTEVLQRIQDLSPHVGQAYTGAALRKTRKEVFSAQRGSRKNQGVPQIAVLVTHRASDDNVTKAAVNLRREGVTVFTMGVEGANPEQLEKIASYPAEQFTSKLSNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGNTQPTDFHEMKIFLSEVVDMFNIAPHKVRVGAVQYADTWDLEFEISKYTNKPDLGKAIDNIRQMGGNTNTGAALNFTLTLLQRAKKQRGNKVPCHLVVLTNGMSQDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGDEKRVYYVHEFDALRDIRNQVVQEICAEEACRDMKADIMFLVDSSGSIGLENFSKMKVFMKNLVSKSQIGADRVQIGVVQFSHENREEFQLNTFMSQNDIANAIDQMAHIGETTLTGSALTFVSQYFSPEKGARPNVRKFLILITDGEAQDIVKDPAVALRKDGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVISVLQNDQPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNATAKALRDKGILVLAVGIAGANTWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFQKMKEFLASVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEKEISTQIEAIQQIFGYTHIGDALRKVKHYFRPDTGSRINAGTPQVLLVLTDGRSQDEVAQAAEELRHKGVDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVKKRIVRNICTSGGESNCFVDVVVGFDISSQRRGQTLLEGQPWMGTYLQDLLRAISSLNGVSCEVGTETQVSIAFQVTNAMERYPSKFEIYSENILSSLQGVTVNGPSRLNPNLLSSLWDTFQNKSAARGKVVLLFSDGLDDGVEKLEQKSDELRKEGLNALITIAVDGAADSSDLADLLYIEFGKGFEYRTQFTIGMGNLGSHLSKQLINVAERTCCCLLCKCTGWDGAMGDPGSAGEKGPPGFKGSEGYLGEEGIAGERGALGPMGEQGTKGCLGAKGPKGARGLSGEEGEVGEDGLDGLNGEQGDSGIPGRRGERGDAGSQGSPGKRGASGDRGPKGLRGDPGTPGRDNSIQGPKGLKGDPGRQGRRGWPGSPGTPGSRRKMVVHGRRGHTGPQGNPGITGPDGLEGSPGLKGPQGPRGEVGEKGEKGGFGMKGPQGPPGPKGRAGNQGHWGSQGSKGEPGDLGEKGAAGFPGPRGLQGDDGSPGYGSIGRKGTKGQEGFPGESGPKGDIGDPGDPGEAGPKGARGKTVSAGIPGEPGSPGEPGPPGRKGMKGARGLASFSTCDLIQYVREHSPVRHGKPECPVHPTELVFVLDQSRDVTEQDFERMKGMMASLVRDIKVRDASCPVGARVAILAYNSHTRHLIRFSDAYRKDQLLREIKALPYEKSSDSREIGKAMRFISRNVFKRTLPGAHVRRIATFFSSGQSADVQSITTAAMEFSALDIIPVVIAFSNVPSVKRAFSIDDTGTFQVLVVPSRPDQVPALERLQRCTFCYDLCKPDASCDQAKPPIQSYLDAAFLLDSSWHVGGAEFEDMRDLLEALLDHFEIASEPETSVTGDRVALLSHAPVDFLPNTQRSPVRTEFNLTTYSSKHLMKRHVEQAVQQLNGDAFLGHALRWALDNVFLNTPNLRRNKVIFVISAGETSHLDAETLKKESLRAKCHGYALFVFSLGPDWDDKELEDLASHPLDQHLIQLGRIHKPDHGYGVKFVKSFINSIRHGINKYPPVNFKAKCSRLSSAAPKLSPRQFRSFVPGPQKALLKDHTLEAAKLFQDKKRLSSILRSGRAALSSHSRSTRYSFKQGREATKATSKLGKRSD

Target ORF information:

RefSeq Version	XM_006226549.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen, type VI, alpha 6 (Col6a6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006226549.3

ORF Insert Sequence:

ATGCTGCTGA TTTTGTTCCT GATGATGATT TGTTTACACG TTTGTGCGAA CCAAGATTCT 
GGCCCCGAGT ACGCAGATGT GGTTTTTCTG GTGGACAGTT CCGATCACCT AGGGATGAAG 
TCCTTTCCTC TTGTGAAAAC TTTCATCCAA AAGCTAGTCA GCAGCCTCCC CGTAGAGGCC 
AACAAGTACC GTGTGGCCCT GGCCCAGTAC AGTGATGCTC TCCACAATGA GTTCCACCTA 
GGCGCCTTCA AGAACAGGAA CCCCATGCTG AACCACCTCA AGAAGAACTT CGGGTTCATC 
GGCGGCTCCC TGAAGATTGG GAACGCCCTG CAGGAGGCTC ACAGGACCTA TTTCTCTGCT 
CCCACAAACG GAAGAGACAA GAAACAGTTC CCCCCAATCC TGGTGGTGCT GGCTTCAGCG 
GAGTCCGAGG ATGACGTAGA AGAGGCTTCG AAGGCCCTGC GGGAAGATGG GGTGAAAATC 
ATCTCTGTGG GGGTGCAGAA GGCTTCTGAG GAAGACCTGA AGGCCATGGC CACCTCTCAG 
TTTCATTTCA ACCTCAGGAC TGCCAGAGAC CTCAGCATGT TTGCCCCAAA CATGACACAG 
ATCATCAAGG ATGTGACTCA GTACAGGGAA GCAATGACTG ATGACATTAT TGTAGAAGCT 
TGCCAAGGCC CATCTGTGGC CGATGTGGTA TTCCTGTTGG ATATGGCCAT CAACGGCAGC 
CAAGAGAACC TAGATCATCT TAAAGCATTC CTGGGGGAAA GTGTCTCTGC CCTTGACATA 
AAGGAAAACT GCATGAGGGT TGGCCTGGTG GCCTATAGCA ACGAAACAAG GGTGATCAGT 
TCTCTGAGCA TGGGTGTCAA CAAGACGGAG GTCTTACAGC GCATACAGGA TCTGTCCCCT 
CACGTAGGGC AGGCCTACAC AGGAGCTGCC CTCAGAAAGA CGAGGAAGGA AGTCTTCAGT 
GCACAGAGGG GCAGTCGGAA GAACCAAGGG GTGCCTCAGA TTGCTGTGCT GGTGACCCAC 
AGAGCTTCAG ATGACAACGT GACCAAGGCA GCTGTCAACC TCCGGCGGGA GGGAGTGACC 
GTCTTTACCA TGGGCGTAGA GGGGGCTAAC CCAGAACAGC TGGAGAAGAT CGCATCCTAC 
CCTGCCGAGC AGTTCACCTC CAAACTGAGC AACTTCTCTG AGCTGGCCAC CCACAACCAG 
ACGTTCCTGA AGAAGCTGAG GAACCAAATC ACACACACAG TCTCTGTCTT CTCAGAACGG 
ACTGAGACCC TCAAATCAGC CTGTGTGGAC ACAGAGGAAG CGGATATCTA TCTGCTCATT 
GATGGTTCAG GGAACACGCA GCCCACAGAC TTCCATGAGA TGAAGATCTT CCTGTCCGAG 
GTGGTGGACA TGTTCAACAT CGCTCCCCAC AAGGTGCGAG TCGGGGCCGT GCAGTACGCC 
GACACCTGGG ACCTGGAATT TGAGATCTCT AAGTATACTA ACAAGCCTGA CTTGGGAAAG 
GCCATCGACA ACATCAGGCA GATGGGTGGA AACACCAATA CGGGTGCAGC CCTGAACTTC 
ACCCTGACGC TGTTGCAAAG AGCAAAGAAG CAACGGGGAA ACAAAGTGCC ATGTCACCTG 
GTTGTCCTGA CAAACGGCAT GTCCCAGGAC AGCGTCCTGG GTCCCGCGCA TAAGCTGAGG 
GAGGAAAACA TCCGTGTGCA TGCGATCGGC GTCAAGGAAG CCAACCAAAC ACAGCTGCGG 
GAGATAGCGG GAGATGAAAA GCGAGTTTAT TACGTGCATG AATTCGATGC CTTGAGGGAC 
ATAAGGAACC AAGTGGTTCA GGAGATCTGT GCTGAAGAAG CCTGCAGAGA TATGAAAGCG 
GACATCATGT TTCTGGTGGA CAGCTCTGGC AGCATTGGAC TCGAAAACTT CAGCAAGATG 
AAGGTGTTTA TGAAGAACCT GGTGAGCAAA TCCCAGATCG GGGCTGACCG GGTGCAAATT 
GGTGTGGTCC AGTTCAGCCA CGAAAACAGG GAGGAGTTTC AGCTCAACAC ATTCATGTCT 
CAAAATGACA TTGCCAACGC CATCGACCAA ATGGCTCACA TTGGAGAGAC AACCTTGACA 
GGCAGTGCGC TGACCTTTGT GTCTCAGTAC TTCAGTCCTG AAAAGGGGGC CCGGCCCAAT 
GTTAGGAAGT TCCTCATTCT CATCACGGAT GGCGAGGCTC AGGACATAGT AAAGGACCCA 
GCAGTAGCCC TTCGAAAAGA CGGTGTGATT ATCTATTCTG TGGGAGTGTT CGGCTCCAAT 
GTCACCCAGC TCGAGGAGAT CAGTGGGAAG CCGGAGATGG TTTTCTATGT TGAGAATTTT 
GACATCCTAC AGCATATTGA AGATGATCTC GTTCTGGGAA TCTGCAGTCC CCGTGAAGAA 
TGCAAGCGGA TTGAAGTTTT GGATGTAGTG TTTGTCATCG ACAGCTCCGG CAGCATTGAC 
TATCAAGAAT ATAACATCAT GAAGGACTTC ATGATTGGCT TGGTGAAAAA AGCTGACGTG 
GGCAAGAATC AGGTCCGGTT TGGAGCTCTG AAATATGCTG ATGACCCAGA GGTGCTGTTT 
TACCTGGATG AACTGGGCAC AAAGCTGGAG GTGATTTCAG TGCTCCAGAA TGACCAGCCC 
ATGGGTGGTA ACACTTACAC TGCTGAGGCC CTGGCTTTCT CCGATCACAT GTTCACCGAA 
GCCCGGGGCA GCCGTCTGCA CAAGGGAGTC CCCCAAGTCC TCATTGTGAT TACTGATGGA 
GAATCTCATG ATGCAGAGAA GCTCAATGCC ACCGCCAAGG CCCTGAGGGA CAAAGGCATT 
CTGGTCCTAG CTGTGGGGAT TGCTGGCGCC AATACCTGGG AGCTCTTGGC CATGGCTGGG 
TCGAGTGACA AGTACTACTT TGTAGAGACC TTCGGAGGCC TGAAGGGGAT ATTTTCCGAT 
GTGTCAGCCA GTGTCTGTAA CTCTTCAAAA GTCGATTGCG AAATTGAAAA AGTTGACCTC 
GTATTCCTCA TGGATGGTTC AAACAGCATC CATCCGGATG ACTTCCAGAA GATGAAGGAG 
TTTTTGGCAT CGGTCGTTCA AGACTTCGAT GTCAGCCTCA ACAGAGTCCG CATAGGAGTG 
GCACAGTTCA GCGACAGCTA CAGGTCAGAG TTTCTGCTGG GGACATTCAC AGGGGAAAAG 
GAGATATCCA CACAGATTGA GGCCATCCAG CAGATCTTTG GTTACACCCA CATTGGAGAT 
GCACTCAGGA AGGTGAAGCA TTACTTTCGG CCAGACACGG GCAGCAGGAT CAACGCCGGT 
ACCCCTCAGG TGCTGCTGGT CCTCACAGAT GGCCGGTCCC AAGATGAGGT GGCCCAGGCG 
GCTGAGGAGC TGAGACACAA GGGTGTGGAC ATCTACTCGG TGGGCATCGG GGACGTGGAT 
GACCAGCAGC TGATCCAGAT AACCGGGACA GCGGAGAAAA AACTGACGGT GCATAACTTC 
GACGAGCTAA AAAAGGTGAA GAAAAGGATC GTTCGTAACA TCTGTACCTC AGGTGGTGAG 
AGCAATTGTT TCGTGGATGT GGTGGTTGGA TTTGATATCT CAAGTCAGCG GAGAGGGCAG 
ACTTTGCTCG AAGGTCAGCC TTGGATGGGG ACCTACCTCC AAGACCTCTT ACGTGCCATC 
AGCTCCCTCA ATGGGGTAAG CTGTGAGGTG GGCACAGAGA CTCAGGTGAG CATAGCTTTT 
CAAGTGACGA ATGCTATGGA GAGATACCCT TCCAAGTTTG AGATCTACAG TGAGAACATA 
CTGAGCAGCC TGCAGGGTGT GACAGTGAAC GGCCCATCTC GCCTCAACCC AAACCTGCTG 
AGTTCTCTGT GGGATACGTT TCAGAACAAG TCCGCTGCTC GAGGGAAGGT GGTCCTTCTG 
TTTTCAGATG GATTGGATGA CGGCGTTGAA AAACTTGAAC AGAAATCTGA TGAACTCAGA 
AAGGAAGGCC TGAATGCCCT CATAACCATC GCTGTGGACG GAGCTGCCGA TTCCAGTGAC 
CTGGCTGACC TTCTCTACAT TGAATTTGGG AAAGGATTTG AGTACAGAAC ACAGTTCACA 
ATCGGAATGG GGAACCTTGG GAGTCACCTG TCAAAGCAAC TAATCAATGT TGCAGAGAGG 
ACCTGCTGCT GTTTGCTCTG CAAGTGCACA GGATGGGACG GCGCCATGGG GGATCCGGGC 
TCAGCAGGGG AAAAGGGACC CCCAGGATTT AAAGGCAGTG AAGGCTACCT GGGAGAAGAA 
GGCATTGCTG GAGAAAGAGG AGCCTTGGGA CCAATGGGAG AGCAAGGTAC AAAGGGATGC 
TTAGGTGCCA AAGGGCCCAA GGGAGCCCGA GGACTCAGCG GAGAAGAGGG TGAAGTTGGG 
GAAGATGGGC TTGACGGATT AAATGGAGAA CAGGGTGACA GTGGGATTCC TGGAAGAAGA 
GGAGAAAGGG GTGATGCGGG ATCTCAGGGA AGCCCAGGCA AGAGAGGGGC TTCTGGTGAC 
CGTGGACCGA AGGGCCTACG AGGAGATCCG GGAACTCCTG GACGTGATAA TAGCATACAG 
GGACCCAAGG GCTTGAAAGG AGACCCTGGA AGACAAGGCA GAAGAGGCTG GCCAGGCTCT 
CCTGGGACAC CAGGCTCAAG AAGAAAGATG GTAGTTCATG GCCGAAGAGG ACATACAGGC 
CCACAGGGAA ATCCAGGCAT CACAGGCCCA GATGGACTTG AAGGTTCGCC CGGACTTAAG 
GGCCCGCAGG GTCCAAGAGG AGAGGTCGGT GAGAAAGGAG AAAAAGGAGG CTTCGGAATG 
AAAGGTCCCC AGGGGCCTCC AGGACCCAAA GGACGAGCTG GGAACCAAGG CCATTGGGGA 
AGCCAAGGAA GCAAAGGAGA ACCTGGAGAC CTGGGGGAAA AGGGAGCTGC TGGCTTTCCA 
GGGCCTCGGG GCCTGCAGGG TGACGACGGC AGCCCAGGAT ATGGTAGCAT TGGCCGAAAA 
GGGACAAAGG GGCAAGAAGG ATTCCCCGGA GAGAGTGGAC CAAAGGGTGA TATTGGGGAC 
CCTGGCGATC CAGGAGAGGC TGGTCCCAAG GGAGCCAGAG GCAAAACGGT ATCTGCTGGG 
ATTCCAGGAG AGCCAGGATC CCCCGGGGAG CCAGGACCCC CTGGAAGGAA GGGCATGAAA 
GGAGCCCGAG GACTGGCTTC ATTTTCCACA TGTGACCTCA TTCAGTATGT GCGGGAACAC 
AGTCCTGTCA GACACGGAAA ACCTGAGTGC CCAGTGCATC CAACTGAGTT GGTGTTTGTC 
CTGGACCAGT CCCGGGATGT CACAGAACAG GACTTTGAGC GGATGAAGGG GATGATGGCC 
TCCTTAGTTA GGGACATCAA AGTTAGGGAT GCCAGCTGCC CCGTGGGTGC CCGCGTTGCC 
ATCCTAGCCT ACAACTCCCA CACCAGGCAC CTCATCCGCT TCTCGGATGC CTACAGGAAG 
GACCAGCTGC TCAGGGAAAT TAAAGCTCTT CCTTACGAGA AATCCTCCGA CAGCAGGGAG 
ATTGGCAAAG CCATGAGGTT TATCTCCAGG AATGTCTTCA AGAGAACCCT CCCAGGGGCT 
CACGTGAGAA GAATCGCCAC GTTCTTTAGC AGTGGCCAGT CAGCCGATGT CCAGTCCATT 
ACCACAGCTG CCATGGAGTT CAGTGCCCTG GACATCATAC CAGTGGTGAT TGCATTCAGC 
AACGTGCCCT CGGTCAAGCG TGCCTTTTCG ATTGATGACA CTGGTACATT CCAAGTCCTT 
GTGGTTCCCT CCAGGCCTGA CCAAGTGCCA GCGTTAGAGA GACTCCAGCG GTGCACTTTC 
TGCTATGACC TATGCAAGCC AGATGCTTCT TGTGACCAAG CCAAACCCCC CATTCAGTCC 
TACCTGGATG CTGCATTCCT GCTGGATAGC TCCTGGCATG TGGGAGGTGC AGAATTTGAA 
GACATGAGAG ACCTCCTGGA GGCCCTGCTA GATCACTTTG AAATCGCTTC AGAGCCAGAG 
ACGTCTGTCA CTGGAGACAG GGTGGCCCTA CTGAGTCATG CTCCCGTTGA CTTCCTACCC 
AACACCCAGA GGAGTCCCGT TAGAACTGAG TTCAACCTAA CCACCTATAG CAGTAAGCAC 
CTCATGAAGA GACACGTGGA GCAAGCAGTC CAGCAGCTGA ATGGAGATGC TTTCCTTGGT 
CATGCCCTCA GGTGGGCACT GGACAATGTC TTTCTAAACA CGCCCAATCT GAGAAGGAAC 
AAAGTCATAT TTGTGATATC TGCTGGGGAA ACCAGCCACC TGGATGCGGA AACCTTAAAG 
AAAGAGTCCC TGCGAGCCAA ATGTCATGGG TATGCCCTCT TTGTGTTTTC CTTGGGCCCT 
GACTGGGATG ACAAAGAACT GGAAGACCTG GCCAGCCACC CACTTGACCA GCACTTGATC 
CAGCTTGGCC GAATTCATAA ACCTGACCAC GGATATGGTG TAAAGTTTGT GAAGTCCTTT 
ATAAACTCCA TCAGGCATGG GATAAACAAG TATCCACCGG TCAACTTTAA AGCCAAGTGC 
AGCAGACTCA GCTCGGCGGC TCCGAAGCTG TCCCCACGCC AGTTTCGAAG CTTTGTTCCG 
GGGCCACAGA AAGCTCTCCT CAAAGACCAC ACACTGGAGG CAGCAAAGCT CTTTCAAGAT 
AAAAAACGCC TTTCAAGTAT CCTGAGAAGT GGCAGGGCTG CTCTTTCAAG TCATTCCAGA 
AGCACACGCT ACTCCTTTAA ACAGGGGAGA GAAGCGACAA AAGCTACTTC TAAACTCGGT 
AAACGAAGTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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Col6a6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

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