col6a5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	col6a5
Entrez Gene ID	100486063
Full Name	collagen alpha-6(VI) chain-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031903527.1	XP_031759387.1	collagen alpha-6(VI) chain isoform X1
XM_012965535.1	XP_012820989.1	collagen alpha-6(VI) chain-like isoform X2
XM_012965535.2	XP_012820989.2	collagen alpha-6(VI) chain isoform X2
XM_012965536.2	XP_012820990.2	collagen alpha-6(VI) chain isoform X3
XM_012965534.1	XP_012820988.1	collagen alpha-6(VI) chain-like isoform X1
XM_012965538.2	XP_012820992.1	collagen alpha-6(VI) chain-like isoform X4
XM_012965536.1	XP_012820990.1	collagen alpha-6(VI) chain-like isoform X3
XM_012965534.2	XP_012820988.2	collagen alpha-6(VI) chain isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL6A6	XP_003310057.1
Bos taurus (cattle)	COL6A6	XP_002685218.2
Gallus gallus (chicken)	COL6A6	XP_426008.4
Xenopus tropicalis (tropical clawed frog)	LOC100486063	XP_002942405.2
Canis lupus familiaris (dog)	COL6A6	XP_853279.3
Mus musculus (house mouse)	Col6a6	NP_001096077.1
	COL6A6	XP_001113364.2
Homo sapiens (human)	COL6A6	NP_001096078.1
Rattus norvegicus (Norway rat)	Col6a6	XP_236593.5
Danio rerio (zebrafish)	col6a6	XP_692457.6

Gene Ontology

The following col6a5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col6a5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa33521	XM_012965538.2 Latest version!	Xenopus tropicalis collagen alpha-6(VI) chain-like (LOC100486063), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa33517	XM_012965534.1 Latest version!	Xenopus tropicalis collagen alpha-6(VI) chain-like (LOC100486063), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa33518	XM_012965535.1 Latest version!	Xenopus tropicalis collagen alpha-6(VI) chain-like (LOC100486063), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa33519	XM_012965536.1 Latest version!	Xenopus tropicalis collagen alpha-6(VI) chain-like (LOC100486063), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa59874	XM_012965534.2 Latest version!	Xenopus tropicalis collagen type VI alpha 5 chain (col6a5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa59874	XM_031903527.1 Latest version!	Xenopus tropicalis collagen type VI alpha 5 chain (col6a5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa59875	XM_012965535.2 Latest version!	Xenopus tropicalis collagen type VI alpha 5 chain (col6a5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa59876	XM_012965536.2 Latest version!	Xenopus tropicalis collagen type VI alpha 5 chain (col6a5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col6a5 ( XM_012965538.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820992.1 Xenopus tropicalis collagen alpha-6(VI) chain-like (LOC100486063), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa33521
Clone ID Related Accession (Same CDS sequence)	XM_012965538.2
Accession Version	XM_012965538.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-6(VI) chain-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965538.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTTT GGAAAGTATT TCTCGCCTGC GTGCTTGGCA GCTACTCAGT CAATGTCTCA 
GCTTCTCAGA AAACAGTGTG CACAGAGGCC AGTATTGCAG ATATTGTATT CCTTATGGAT 
GGATCCTGGA GCATCGGAAC GGAAAACTTT ATCACAATGA AGAACTTTTT GTACACTTTG 
GTTAATGGTT TTGATGTTGG CCTAGATAAG ATCCAGATTG GCCTAATCCA GTACAGTGAT 
AATGCACGTA CAGAATTCTT CCTAAACAGT TACAGTAACA AAGAAGATGT TTTGAAATAT 
ATTCAGAATC TAAAGTATAA AGGGGGTGGG ACAAAGACTG GCTTAAGTCT GGAGTTCATG 
CTGACGCAGC ACTTTAGTGA AGCTGCAGGC AGCAGAGCAG CAGAAGGTGT GCCTCAGATT 
GCAGTCGTTA TAACTGATGG ACAAGCTCAA GATAGCATCA GGGAGCCTGC CATTGCAGTG 
AAGAACGCTG GAATCATACT GTATGCCATT GGCATTAAAG ATGCAGTTTT ATCTGAGCTT 
AATGAAATAG CCAGTGATCC AGATGACAAA CATGTTTATA GCGTTGCAGA CTTTAATGCA 
CTGCAGAGTA TTTCCCAAAA CATGATCCAG GTGCTCTGCA CAACTGTAGA AGAAGCAGCA 
AGACAGACAG GTCAAATTGC TCAAGTTTGC AGAACTGCCA ATCAAGCTGA CATTGTGCTG 
CTAGTCGAAA GCACAACAAG AATGGGTGAC GCTACTTTTG AAAAAGCAAA GAATTTTCTT 
TATGATTTAG TTTCTAATTT GGATGTTGGA ATTAATAAGA TTCGCATTGG GCTTGTAACC 
TACAATGATG AGACAAATCC AGAATTTTTG CTAAACAGCT ATTCTTCGAA AACTGAAATC 
TTAGAAAGTA TTCAGAATAT GAAATATGTA GAAGGATATA CCTACACAGG GAGAGCCCTT 
GAGTATGTAA ATACAACCTA CTTTACACAG GCAGCAGGCA GTAGATTTGA AGAGAGCGTC 
GCTCAAATTC TTATCATTGT GACAGAAGGA GACTCTTCAG ATACCCTCAC TGAGCCTGCT 
AAAGAACTCA AATCAAGAGG CATATCTGTT TATGTGGTTG GGACAAACAT CAAGTACGAT 
AGACAGCTTC AGGAAGCATC AAGCAAGCCC GACGAAAAGT TCTTTTATCA ACTCGATGAT 
TTTGATGACT CTGAGAATGT CACTGAGCAG CTGTTAAAAA ACCTGTGCTT TAGCATAGAT 
TTGAATATTC AAGTATATTC AAAGCGCTAC GCTGATGTTG TATTTCTAGT CGATTCTTCT 
ACAAGCATGG GAACCATCTT TTTTCAAAAG ATGAAGGATT TCATCATACA CATAATAAAC 
CAGTTAAATG TTGGAATAAA TAAACACCGT ATAGGACTGG CACAGTACAG TGGTCTACCC 
CAAACTGAAT TTCTACTGAA TCATTATGAA ACCAAAGAGG AGATACTAAA GCACATTAAG 
GAAACATTCA CATACAGAGG TGGCCCCTTA AAAACAGGAC ATGCTTTGGA ATTTGTACGC 
TCAACATTTT TCATAGAAGA GGCTGGGAGT AGGATTAACT ACGGCAACCC CCAGTTCTTA 
GTGGTCATAA CATCATCAAA ATCAGAGGAT GCTGTTAGAA GACATGCCGA GGAACTCAAA 
AGTGTTGGGG TCACAACCAT CTCTGTTGGC ATTGGCAATT CTGATCGAAA AGAACTGGAA 
AAAATTGCTA CTGATCCTTT TGTTTTCCAG ACCACAGGTT TACAGCACAT TTCAAATTTA 
CAGCAGGATG TGGCCAATGT TATTATAGCA GAAGACATGC TACAGTTTTC CCCAGTATCA 
GTTGTACCAG CAGTTTGCTC CAGTGCTTCT GTAGCTGACA TTGTGTTTCT AATTGATGAA 
TCTAGCAGCA TTGGCCCCAT CAACTTTCAA CTAACCAGAG TCTTTCTACA CAAGGTTGTT 
AGTGCACTGG ATATTTCCTT GAGTAATGTT AGAGTGGGCT TAGTGCTTTA CAGTGATGAG 
CCAAGACTTG AACTTAAACT GAACACTTTT AATGAAAAAT ATGAAATTTT GGACTTCATA 
ACTAAGCTGC CATACAGAGG AGGCAAAGCA CATACAGGTG CTGCGCTGGA CTTTCTAAGG 
AAAAAAATGT TCACCAAACA AAATGGCGGC CGGCCACATC AAGGAGTTCA GCAGATAGCG 
GTGGTTATGA CTAATGGGCA GTCCATGGAT AATTTTACTA AACCTGCTGC AAAACTGAGA 
CGGTCTGGCG TGGAGGTTTT TGCTGTTGGC TTTCAGAATA TCAATGATAC AGAACTGGAT 
ATCATAGCAT CTCACCCACC GCGAAAACAT GTCACAAATG TGGAATCTTT CCTGCAGCTA 
TCCAACCTTG AGTTCAGAAT ACAGAAACGA CTTTGCAATG AAATTGTTGT GAAGTCATTT 
GCAGTACCTG CAATTGCTCG TGCAGTGAAG GAAGGGTGTG TGGATACAGA AGAAGCAGAT 
ATATACTTTC TAATTGATGG TTCTGGCAGT ATATATCCAG AGGACTTTGA AGATATGAAA 
AAATTCATGA TTGAATTAAT AAGCATGTTT CAAGTTGGTG CCAACAGAGT ACGCTTTGGA 
GTTGTCCAAT ACTCAGATGT TCGTAGAACT GAATTTTTCA TCAGTGAACA CAACACCCAG 
AAAATGTTAA AGGATGCTAT TTCTCAAATA GAACAGTTGG GCGGTGGTAC CCTAACAGGA 
GAAGCTTTAA CGTCAATGAA GCAACTTTTT GTTAATGCAG CTAAAGATCG CCCTCATAAA 
GTACCCCAGT CATTGGTTGT CATTACAGAT GGAGAATCTC AAGACCGGGT CACAGAAGCA 
GCTGCAGAAA TTAGAAATGA TGGCATTACT ATATTTGCAA TTGGTGTTAA AAATGCAGTG 
GAAGAAGAGA TTAGAGATAT AGCAGGGTCA AATGAGAAGA TGTTTTTTGT TAACAACTTT 
GATTCCCTGA AGGTGATCAA GAATGATTTG GCTCGTGAAT TGTGTACACC TGAGGCATGT 
AAGAACATGA AAGCTGATAT CGTGTTTCTG GTTGATAGCT CTGCAAGCAT TAACAGTGAT 
GATTATGAAA CAATGAAAGA ATTCATGGAG TCAATGGTCA AGCAGGCAGA AATCGGCCCT 
GATAGAGTCC AGATTGGTTT AATTCAGTTT AGCTCTGAAA CAAAGGAAGA ATTTCCTCTC 
AACAGGTACA AAAGAAAGGA TGAAATACAA TCAGCAATAA GAGGCATCCA ACAACTGAGC 
CAGGGAACCC TTATGGGGGA AGCCCTTAAA TATACATTAC CTTATTTTAG TGCTTCCAAA 
GGAGGCCGTG TAAATACCAA ACAATATCTG ATCGTCATAA CAGATGGTGA GGCCCAGGAT 
GCTGTGGGAA ACCCAGCCAA AGCAATAAGG GACCATGGAG TTATCATTTA TGCCATAGGA 
GTACAACAAG CCAACAACAC ACAACTTCTG GAGATTGCTG GCAAACAAGA GCAGGTGTAT 
TATGAAGATA GCTTTGATTC CCTAGCTTTT TTGAACAAGA ACATCATGTT TGAGATTTGC 
AATCCTCAAG AATCTTGCAA AAAGACTGAG GTGGCAGACA TCATATTTCT GTTGGATGCT 
TCTGCAAGCA TAACCAGAGG AGAGTTTAGA CTCATGCAGA GATTTGTGGA GGCTGTTGTC 
AATGACTCTT TGGTTGGAAA GGATAATGTT CAATTTGGAG CAGTTGTTTA CGGCACAAAT 
CCAGCAGAAC AGTTTTCACT CAATACGTAT TCAACTAAAC TTGATATTCT TAAGGCTGTA 
TTTAGTTTAC CCCAAGTATC TGGATACACA TACACTGCAA AAGCGCTGGA ATACACTCGG 
ATACGGTTTG GTACATCATA TGGTGGAAGA CCAGGAATTT CACATATTCT AATTCTAGTC 
ACAGATGGAG CCACTACTGA AGCTGATCGC CCTAACCTAC CAATAGTATC AAAGGCTTTG 
AAGGATGATG GCATCATTGT CTTTGCAGTA GGAGTTGGCA AGGCAGTTCC TCAAGAGCTT 
CAGCAGATTG CAGGATACCC AGACAGATGG TTCTTAGTCC AGAACTACAA AGGCCTGGAC 
AATATACATG ATAATATAAC ACAGGTTGTA TGTGATGAAT CAAAACCAGC TTGCTCACAT 
GAACAGCTTG ATCTTGTTTT CCTGATTGAC GGCTCAGCAA GTATCACTTC TAGCAACTTC 
ACAAGTGCCA AGACTTTCAT GAAAGAAATC GTTGACAGCT TTACAATTTC AGAGAACAGA 
GTGCGTATTG GAGTCGCACA GTACAGTGCA AATCCCAAGA AAGAATTCTT CTTAAATGAG 
TACTACTCCA GCTCCGACAT GAAGAAACAG ATTGATAGCA TCTCCCAACT GAAAGCTACC 
ACTTACACTG GCAAAGGCCT ACGATTTGTC AAACAGTTCT TTGATCCAGC AAATGGCGGC 
CGTAAGAATG TTCCGCAGTA CCTGATAGTC ATGACTGATG GCATGTCTAA TGACAGCGTA 
AATGAAGATG CTGCTGCTTT GCGAAGCAGT GGAGTAAAGA TCTTTTCCAT TGGTATTGGT 
CTGCGGAACA GTTTTGAGCT TGTTATGATT GCCGGGTCAC CCAAAAATGT TTACGAAGTG 
GAAACGTTCC AAGCGCTGGA CTCTATAAAG AGGCAGATTG TGGCACAAGT CTGTGAGCCA 
TCTGATCAGC CTATTATAGG TAAAGAATGC AGCTTAGATA TCACCATATC CGTTGACTAC 
TCAAGAAGAG CAATGCTATC TACTGTCCCC CGTGTGCAGC AGAAATTGCA GCAGTTCCTC 
CCTCCGGTGC TTCAGCGCAT TTCCTCGCTT AGCAATATCA GCTGTGCCTC TGGATCTCAA 
ATTAACTTAC GCTTCAGATA CGCTGTTGCA GCACAGGACA GACTGACATT TGATTCCGAC 
TTTGAGAAAT TTAACGAGGA CATCATTAAG AAGTTTATAG CTGAACAGTC TTCTGTTGAT 
ACATATTTAA ATGCAAAGTT TCTTCAGTCC CTCTGGAACA AATACATAGG TTTGCCGTCT 
CAGAAAGCAA AAATTATTCT GGTTTTTACT GATGGACTGG ATGAAAGTTC TGAAGTACTC 
AAGGCGACTA CTGAATCTCT TCGGAAACAA GGGTTGGATG CACTGTTATT CATTGCTCTT 
GAGGGGGTGA AGGATTTTAC AGAGCTGCAA AACTTAGAGT TTGGAAGAGG ATTTTCTTAC 
CAGCAACCTC TGAGCATTGC GCTGCATGAT CTGCCAAGTG TAATGCTAAA AGAAATGGAT 
ACCGTTGCAG AAAGGAAATG CTGTAATGTA GTTTGTAAAT GTTTAGGACA AGATGGACCT 
CTAGGCTCGC CAGGGGCTTT AGGAACAAAG GGTCAGCCTG GTATAAAGGG ATCTCAAGGT 
CATCCTGGAG AGGAAGGCGG TCATGGTGAA AGAGGATTCC GTGGGCTGAA TGGGACAAGA 
GGCGAAAGTG GATGTCCTGG TCGTAGGGGA TTAAAGGGTG CCAGAGGTAT TCGTGGTGAC 
AAGGGTGATG ATGGAGAGTT TGGAATTGAT GGTGTGCCTG GAGAGCAGGG TGAACCTGGA 
GGGCGTGGAG CATCAGGAGA ACGTGGAGAC ACTGGAGCTC AGGGCAGAAA AGGTCCTCGT 
GGGCAACCTG GAGAAAAAGG GGAAAATGGA CTGAGGGGAG ACCCAGGTGA GCCTGGAACT 
AACATTAACA TTCAGGGACC TAAAGGACTG AAAGGAAACC CAGGACGTCA GGGAGAGGCG 
GGAAGTGACG GTGTGGAAGG AGAGCCAGGA AATAATGGAC CTAATGGTCC ACAAGGCCGA 
AGAGGGCCTC CTGGTTTAAA GGGAGCACGA GGATTTCCTG GAGAGCCAGG GAACAAAGGT 
GATAGTGGAA TCCAAGGTAT ACAGGGTTCC CAAGGCATGC CTGGACCACC AGGTCCACAG 
GGACCACAAG GGTTATCTGG ACGTCAGGGA GATGCAGGTG CCCCTGGTGC ATCAGGTTCC 
TTTGGCAAAC CCGGAGCTCC AGGACTAAAG GGGGAACCTG GAGAAAACGG AGAGAAGGGT 
GAGCCAGGCA TTCCGGGTCG CAGAGGTTTG CCAGGGCTGG ATGGTCCTGA TGGATATGGT 
CCCCCAGGCT TAAAGGGAGA GAAGGGTCAT TTAGGGTTTC CTGGGGATCC TGGGCCTCAG 
GGAGAAGATG GTGATCCTGG AATAGCTGGA AATGGTGGCC CAAAAGGAAT TAGTGGAAGA 
AGGGGTAATT CTGGCCCTCC AGGAACTAAT GGACAAAGGG GTGAGAGGGG ACCACGTGGA 
CCTATGGGCG CCAAAGGATT GCCAGGAACT ATGCCTATGA CGCCTTGTGA ACTTGTGGCA 
TTTACTCGAG AGAATTGTCC TTGCTTCGCA AGTGCTTCAA AATGTCCAGT TTACCCAACT 
GAAATAGTGT TTGCTCTGGA TGCATCTCAG GACGTTCCCC CGGCTGCTTT CCAAAGGATG 
AAGAACACCA TTATTTCCAT AATAGAAAAG CTGGACATTA GTGAGAGCAA TTGCCCTAGA 
GGAGCCCGTG TTGCTGTTGT TGCGTATAAC AGTAATACCA AATACCTCAT TCGTTTCTCT 
GACTACAAAC GGAAAAGCCT ACTGCTGGAA GCAATTCGTG GCATCTCTCC AGAAAGATCA 
TCTTCAAGGA GAAATATTGG AGAAGCCATG AAGTTTGTAG GGAGGAACAT ATTCAAACGC 
ATTCGTCATG GAATCCATGT TAGAAAAGTG GCTGTGTTCT TTGCTAATGG CCCATCCCCA 
GATGCAGATG CAATAAACTC GGCAGTGCTG GAATACAGTG CTCTTGACAT TATTCCTATT 
GTGATTGCAT TTAATAATGT TCCCAATGTC GTAGCAGCTT TTTCGATAAG TGTACACCTG 
ATGAAGAATG TGAGCTGGAT CAAATGCCTG CAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820992.1
CDS	129..7127
Translation	MGFWKVFLACVLGSYSVNVSASQKTVCTEASIADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREPAIAVKNAGIILYAIGIKDAVLSELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEPAKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRRHAEELKSVGVTTISVGIGNSDRKELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKPAAKLRRSGVEVFAVGFQNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELCTPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNPAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNPQESCKKTEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVPQELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVNEDAAALRSSGVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQVCEPSDQPIIGKECSLDITISVDYSRRAMLSTVPRVQQKLQQFLPPVLQRISSLSNISCASGSQINLRFRYAVAAQDRLTFDSDFEKFNEDIIKKFIAEQSSVDTYLNAKFLQSLWNKYIGLPSQKAKIILVFTDGLDESSEVLKATTESLRKQGLDALLFIALEGVKDFTELQNLEFGRGFSYQQPLSIALHDLPSVMLKEMDTVAERKCCNVVCKCLGQDGPLGSPGALGTKGQPGIKGSQGHPGEEGGHGERGFRGLNGTRGESGCPGRRGLKGARGIRGDKGDDGEFGIDGVPGEQGEPGGRGASGERGDTGAQGRKGPRGQPGEKGENGLRGDPGEPGTNINIQGPKGLKGNPGRQGEAGSDGVEGEPGNNGPNGPQGRRGPPGLKGARGFPGEPGNKGDSGIQGIQGSQGMPGPPGPQGPQGLSGRQGDAGAPGASGSFGKPGAPGLKGEPGENGEKGEPGIPGRRGLPGLDGPDGYGPPGLKGEKGHLGFPGDPGPQGEDGDPGIAGNGGPKGISGRRGNSGPPGTNGQRGERGPRGPMGAKGLPGTMPMTPCELVAFTRENCPCFASASKCPVYPTEIVFALDASQDVPPAAFQRMKNTIISIIEKLDISESNCPRGARVAVVAYNSNTKYLIRFSDYKRKSLLLEAIRGISPERSSSRRNIGEAMKFVGRNIFKRIRHGIHVRKVAVFFANGPSPDADAINSAVLEYSALDIIPIVIAFNNVPNVVAAFSISVHLMKNVSWIKCLQP

Target ORF information:

RefSeq Version	XM_012965538.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen alpha-6(VI) chain-like (LOC100486063), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965538.2

ORF Insert Sequence:

ATGGGATTTT GGAAAGTATT TCTCGCCTGC GTGCTTGGCA GCTACTCAGT CAATGTCTCA 
GCTTCTCAGA AAACAGTGTG CACAGAGGCC AGTATTGCAG ATATTGTATT CCTTATGGAT 
GGATCCTGGA GCATCGGAAC GGAAAACTTT ATCACAATGA AGAACTTTTT GTACACTTTG 
GTTAATGGTT TTGATGTTGG CCTAGATAAG ATCCAGATTG GCCTAATCCA GTACAGTGAT 
AATGCACGTA CAGAATTCTT CCTAAACAGT TACAGTAACA AAGAAGATGT TTTGAAATAT 
ATTCAGAATC TAAAGTATAA AGGGGGTGGG ACAAAGACTG GCTTAAGTCT GGAGTTCATG 
CTGACGCAGC ACTTTAGTGA AGCTGCAGGC AGCAGAGCAG CAGAAGGTGT GCCTCAGATT 
GCAGTCGTTA TAACTGATGG ACAAGCTCAA GATAGCATCA GGGAGCCTGC CATTGCAGTG 
AAGAACGCTG GAATCATACT GTATGCCATT GGCATTAAAG ATGCAGTTTT ATCTGAGCTT 
AATGAAATAG CCAGTGATCC AGATGACAAA CATGTTTATA GCGTTGCAGA CTTTAATGCA 
CTGCAGAGTA TTTCCCAAAA CATGATCCAG GTGCTCTGCA CAACTGTAGA AGAAGCAGCA 
AGACAGACAG GTCAAATTGC TCAAGTTTGC AGAACTGCCA ATCAAGCTGA CATTGTGCTG 
CTAGTCGAAA GCACAACAAG AATGGGTGAC GCTACTTTTG AAAAAGCAAA GAATTTTCTT 
TATGATTTAG TTTCTAATTT GGATGTTGGA ATTAATAAGA TTCGCATTGG GCTTGTAACC 
TACAATGATG AGACAAATCC AGAATTTTTG CTAAACAGCT ATTCTTCGAA AACTGAAATC 
TTAGAAAGTA TTCAGAATAT GAAATATGTA GAAGGATATA CCTACACAGG GAGAGCCCTT 
GAGTATGTAA ATACAACCTA CTTTACACAG GCAGCAGGCA GTAGATTTGA AGAGAGCGTC 
GCTCAAATTC TTATCATTGT GACAGAAGGA GACTCTTCAG ATACCCTCAC TGAGCCTGCT 
AAAGAACTCA AATCAAGAGG CATATCTGTT TATGTGGTTG GGACAAACAT CAAGTACGAT 
AGACAGCTTC AGGAAGCATC AAGCAAGCCC GACGAAAAGT TCTTTTATCA ACTCGATGAT 
TTTGATGACT CTGAGAATGT CACTGAGCAG CTGTTAAAAA ACCTGTGCTT TAGCATAGAT 
TTGAATATTC AAGTATATTC AAAGCGCTAC GCTGATGTTG TATTTCTAGT CGATTCTTCT 
ACAAGCATGG GAACCATCTT TTTTCAAAAG ATGAAGGATT TCATCATACA CATAATAAAC 
CAGTTAAATG TTGGAATAAA TAAACACCGT ATAGGACTGG CACAGTACAG TGGTCTACCC 
CAAACTGAAT TTCTACTGAA TCATTATGAA ACCAAAGAGG AGATACTAAA GCACATTAAG 
GAAACATTCA CATACAGAGG TGGCCCCTTA AAAACAGGAC ATGCTTTGGA ATTTGTACGC 
TCAACATTTT TCATAGAAGA GGCTGGGAGT AGGATTAACT ACGGCAACCC CCAGTTCTTA 
GTGGTCATAA CATCATCAAA ATCAGAGGAT GCTGTTAGAA GACATGCCGA GGAACTCAAA 
AGTGTTGGGG TCACAACCAT CTCTGTTGGC ATTGGCAATT CTGATCGAAA AGAACTGGAA 
AAAATTGCTA CTGATCCTTT TGTTTTCCAG ACCACAGGTT TACAGCACAT TTCAAATTTA 
CAGCAGGATG TGGCCAATGT TATTATAGCA GAAGACATGC TACAGTTTTC CCCAGTATCA 
GTTGTACCAG CAGTTTGCTC CAGTGCTTCT GTAGCTGACA TTGTGTTTCT AATTGATGAA 
TCTAGCAGCA TTGGCCCCAT CAACTTTCAA CTAACCAGAG TCTTTCTACA CAAGGTTGTT 
AGTGCACTGG ATATTTCCTT GAGTAATGTT AGAGTGGGCT TAGTGCTTTA CAGTGATGAG 
CCAAGACTTG AACTTAAACT GAACACTTTT AATGAAAAAT ATGAAATTTT GGACTTCATA 
ACTAAGCTGC CATACAGAGG AGGCAAAGCA CATACAGGTG CTGCGCTGGA CTTTCTAAGG 
AAAAAAATGT TCACCAAACA AAATGGCGGC CGGCCACATC AAGGAGTTCA GCAGATAGCG 
GTGGTTATGA CTAATGGGCA GTCCATGGAT AATTTTACTA AACCTGCTGC AAAACTGAGA 
CGGTCTGGCG TGGAGGTTTT TGCTGTTGGC TTTCAGAATA TCAATGATAC AGAACTGGAT 
ATCATAGCAT CTCACCCACC GCGAAAACAT GTCACAAATG TGGAATCTTT CCTGCAGCTA 
TCCAACCTTG AGTTCAGAAT ACAGAAACGA CTTTGCAATG AAATTGTTGT GAAGTCATTT 
GCAGTACCTG CAATTGCTCG TGCAGTGAAG GAAGGGTGTG TGGATACAGA AGAAGCAGAT 
ATATACTTTC TAATTGATGG TTCTGGCAGT ATATATCCAG AGGACTTTGA AGATATGAAA 
AAATTCATGA TTGAATTAAT AAGCATGTTT CAAGTTGGTG CCAACAGAGT ACGCTTTGGA 
GTTGTCCAAT ACTCAGATGT TCGTAGAACT GAATTTTTCA TCAGTGAACA CAACACCCAG 
AAAATGTTAA AGGATGCTAT TTCTCAAATA GAACAGTTGG GCGGTGGTAC CCTAACAGGA 
GAAGCTTTAA CGTCAATGAA GCAACTTTTT GTTAATGCAG CTAAAGATCG CCCTCATAAA 
GTACCCCAGT CATTGGTTGT CATTACAGAT GGAGAATCTC AAGACCGGGT CACAGAAGCA 
GCTGCAGAAA TTAGAAATGA TGGCATTACT ATATTTGCAA TTGGTGTTAA AAATGCAGTG 
GAAGAAGAGA TTAGAGATAT AGCAGGGTCA AATGAGAAGA TGTTTTTTGT TAACAACTTT 
GATTCCCTGA AGGTGATCAA GAATGATTTG GCTCGTGAAT TGTGTACACC TGAGGCATGT 
AAGAACATGA AAGCTGATAT CGTGTTTCTG GTTGATAGCT CTGCAAGCAT TAACAGTGAT 
GATTATGAAA CAATGAAAGA ATTCATGGAG TCAATGGTCA AGCAGGCAGA AATCGGCCCT 
GATAGAGTCC AGATTGGTTT AATTCAGTTT AGCTCTGAAA CAAAGGAAGA ATTTCCTCTC 
AACAGGTACA AAAGAAAGGA TGAAATACAA TCAGCAATAA GAGGCATCCA ACAACTGAGC 
CAGGGAACCC TTATGGGGGA AGCCCTTAAA TATACATTAC CTTATTTTAG TGCTTCCAAA 
GGAGGCCGTG TAAATACCAA ACAATATCTG ATCGTCATAA CAGATGGTGA GGCCCAGGAT 
GCTGTGGGAA ACCCAGCCAA AGCAATAAGG GACCATGGAG TTATCATTTA TGCCATAGGA 
GTACAACAAG CCAACAACAC ACAACTTCTG GAGATTGCTG GCAAACAAGA GCAGGTGTAT 
TATGAAGATA GCTTTGATTC CCTAGCTTTT TTGAACAAGA ACATCATGTT TGAGATTTGC 
AATCCTCAAG AATCTTGCAA AAAGACTGAG GTGGCAGACA TCATATTTCT GTTGGATGCT 
TCTGCAAGCA TAACCAGAGG AGAGTTTAGA CTCATGCAGA GATTTGTGGA GGCTGTTGTC 
AATGACTCTT TGGTTGGAAA GGATAATGTT CAATTTGGAG CAGTTGTTTA CGGCACAAAT 
CCAGCAGAAC AGTTTTCACT CAATACGTAT TCAACTAAAC TTGATATTCT TAAGGCTGTA 
TTTAGTTTAC CCCAAGTATC TGGATACACA TACACTGCAA AAGCGCTGGA ATACACTCGG 
ATACGGTTTG GTACATCATA TGGTGGAAGA CCAGGAATTT CACATATTCT AATTCTAGTC 
ACAGATGGAG CCACTACTGA AGCTGATCGC CCTAACCTAC CAATAGTATC AAAGGCTTTG 
AAGGATGATG GCATCATTGT CTTTGCAGTA GGAGTTGGCA AGGCAGTTCC TCAAGAGCTT 
CAGCAGATTG CAGGATACCC AGACAGATGG TTCTTAGTCC AGAACTACAA AGGCCTGGAC 
AATATACATG ATAATATAAC ACAGGTTGTA TGTGATGAAT CAAAACCAGC TTGCTCACAT 
GAACAGCTTG ATCTTGTTTT CCTGATTGAC GGCTCAGCAA GTATCACTTC TAGCAACTTC 
ACAAGTGCCA AGACTTTCAT GAAAGAAATC GTTGACAGCT TTACAATTTC AGAGAACAGA 
GTGCGTATTG GAGTCGCACA GTACAGTGCA AATCCCAAGA AAGAATTCTT CTTAAATGAG 
TACTACTCCA GCTCCGACAT GAAGAAACAG ATTGATAGCA TCTCCCAACT GAAAGCTACC 
ACTTACACTG GCAAAGGCCT ACGATTTGTC AAACAGTTCT TTGATCCAGC AAATGGCGGC 
CGTAAGAATG TTCCGCAGTA CCTGATAGTC ATGACTGATG GCATGTCTAA TGACAGCGTA 
AATGAAGATG CTGCTGCTTT GCGAAGCAGT GGAGTAAAGA TCTTTTCCAT TGGTATTGGT 
CTGCGGAACA GTTTTGAGCT TGTTATGATT GCCGGGTCAC CCAAAAATGT TTACGAAGTG 
GAAACGTTCC AAGCGCTGGA CTCTATAAAG AGGCAGATTG TGGCACAAGT CTGTGAGCCA 
TCTGATCAGC CTATTATAGG TAAAGAATGC AGCTTAGATA TCACCATATC CGTTGACTAC 
TCAAGAAGAG CAATGCTATC TACTGTCCCC CGTGTGCAGC AGAAATTGCA GCAGTTCCTC 
CCTCCGGTGC TTCAGCGCAT TTCCTCGCTT AGCAATATCA GCTGTGCCTC TGGATCTCAA 
ATTAACTTAC GCTTCAGATA CGCTGTTGCA GCACAGGACA GACTGACATT TGATTCCGAC 
TTTGAGAAAT TTAACGAGGA CATCATTAAG AAGTTTATAG CTGAACAGTC TTCTGTTGAT 
ACATATTTAA ATGCAAAGTT TCTTCAGTCC CTCTGGAACA AATACATAGG TTTGCCGTCT 
CAGAAAGCAA AAATTATTCT GGTTTTTACT GATGGACTGG ATGAAAGTTC TGAAGTACTC 
AAGGCGACTA CTGAATCTCT TCGGAAACAA GGGTTGGATG CACTGTTATT CATTGCTCTT 
GAGGGGGTGA AGGATTTTAC AGAGCTGCAA AACTTAGAGT TTGGAAGAGG ATTTTCTTAC 
CAGCAACCTC TGAGCATTGC GCTGCATGAT CTGCCAAGTG TAATGCTAAA AGAAATGGAT 
ACCGTTGCAG AAAGGAAATG CTGTAATGTA GTTTGTAAAT GTTTAGGACA AGATGGACCT 
CTAGGCTCGC CAGGGGCTTT AGGAACAAAG GGTCAGCCTG GTATAAAGGG ATCTCAAGGT 
CATCCTGGAG AGGAAGGCGG TCATGGTGAA AGAGGATTCC GTGGGCTGAA TGGGACAAGA 
GGCGAAAGTG GATGTCCTGG TCGTAGGGGA TTAAAGGGTG CCAGAGGTAT TCGTGGTGAC 
AAGGGTGATG ATGGAGAGTT TGGAATTGAT GGTGTGCCTG GAGAGCAGGG TGAACCTGGA 
GGGCGTGGAG CATCAGGAGA ACGTGGAGAC ACTGGAGCTC AGGGCAGAAA AGGTCCTCGT 
GGGCAACCTG GAGAAAAAGG GGAAAATGGA CTGAGGGGAG ACCCAGGTGA GCCTGGAACT 
AACATTAACA TTCAGGGACC TAAAGGACTG AAAGGAAACC CAGGACGTCA GGGAGAGGCG 
GGAAGTGACG GTGTGGAAGG AGAGCCAGGA AATAATGGAC CTAATGGTCC ACAAGGCCGA 
AGAGGGCCTC CTGGTTTAAA GGGAGCACGA GGATTTCCTG GAGAGCCAGG GAACAAAGGT 
GATAGTGGAA TCCAAGGTAT ACAGGGTTCC CAAGGCATGC CTGGACCACC AGGTCCACAG 
GGACCACAAG GGTTATCTGG ACGTCAGGGA GATGCAGGTG CCCCTGGTGC ATCAGGTTCC 
TTTGGCAAAC CCGGAGCTCC AGGACTAAAG GGGGAACCTG GAGAAAACGG AGAGAAGGGT 
GAGCCAGGCA TTCCGGGTCG CAGAGGTTTG CCAGGGCTGG ATGGTCCTGA TGGATATGGT 
CCCCCAGGCT TAAAGGGAGA GAAGGGTCAT TTAGGGTTTC CTGGGGATCC TGGGCCTCAG 
GGAGAAGATG GTGATCCTGG AATAGCTGGA AATGGTGGCC CAAAAGGAAT TAGTGGAAGA 
AGGGGTAATT CTGGCCCTCC AGGAACTAAT GGACAAAGGG GTGAGAGGGG ACCACGTGGA 
CCTATGGGCG CCAAAGGATT GCCAGGAACT ATGCCTATGA CGCCTTGTGA ACTTGTGGCA 
TTTACTCGAG AGAATTGTCC TTGCTTCGCA AGTGCTTCAA AATGTCCAGT TTACCCAACT 
GAAATAGTGT TTGCTCTGGA TGCATCTCAG GACGTTCCCC CGGCTGCTTT CCAAAGGATG 
AAGAACACCA TTATTTCCAT AATAGAAAAG CTGGACATTA GTGAGAGCAA TTGCCCTAGA 
GGAGCCCGTG TTGCTGTTGT TGCGTATAAC AGTAATACCA AATACCTCAT TCGTTTCTCT 
GACTACAAAC GGAAAAGCCT ACTGCTGGAA GCAATTCGTG GCATCTCTCC AGAAAGATCA 
TCTTCAAGGA GAAATATTGG AGAAGCCATG AAGTTTGTAG GGAGGAACAT ATTCAAACGC 
ATTCGTCATG GAATCCATGT TAGAAAAGTG GCTGTGTTCT TTGCTAATGG CCCATCCCCA 
GATGCAGATG CAATAAACTC GGCAGTGCTG GAATACAGTG CTCTTGACAT TATTCCTATT 
GTGATTGCAT TTAATAATGT TCCCAATGTC GTAGCAGCTT TTTCGATAAG TGTACACCTG 
ATGAAGAATG TGAGCTGGAT CAAATGCCTG CAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col6a5 ( XM_012965534.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820988.1 Xenopus tropicalis collagen alpha-6(VI) chain-like (LOC100486063), transcript variant X1, mRNA.

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CloneID	OXa33517
Clone ID Related Accession (Same CDS sequence)	XM_012965534.1
Accession Version	XM_012965534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-6(VI) chain-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTTT GGAAAGTATT TCTCGCCTGC GTGCTTGGCA GCTACTCAGT CAATGTCTCA 
GCTTCTCAGA AAACAGTGTG CACAGAGGCC AGTATTGCAG ATATTGTATT CCTTATGGAT 
GGATCCTGGA GCATCGGAAC GGAAAACTTT ATCACAATGA AGAACTTTTT GTACACTTTG 
GTTAATGGTT TTGATGTTGG CCTAGATAAG ATCCAGATTG GCCTAATCCA GTACAGTGAT 
AATGCACGTA CAGAATTCTT CCTAAACAGT TACAGTAACA AAGAAGATGT TTTGAAATAT 
ATTCAGAATC TAAAGTATAA AGGGGGTGGG ACAAAGACTG GCTTAAGTCT GGAGTTCATG 
CTGACGCAGC ACTTTAGTGA AGCTGCAGGC AGCAGAGCAG CAGAAGGTGT GCCTCAGATT 
GCAGTCGTTA TAACTGATGG ACAAGCTCAA GATAGCATCA GGGAGCCTGC CATTGCAGTG 
AAGAACGCTG GAATCATACT GTATGCCATT GGCATTAAAG ATGCAGTTTT ATCTGAGCTT 
AATGAAATAG CCAGTGATCC AGATGACAAA CATGTTTATA GCGTTGCAGA CTTTAATGCA 
CTGCAGAGTA TTTCCCAAAA CATGATCCAG GTGCTCTGCA CAACTGTAGA AGAAGCAGCA 
AGACAGACAG GTCAAATTGC TCAAGTTTGC AGAACTGCCA ATCAAGCTGA CATTGTGCTG 
CTAGTCGAAA GCACAACAAG AATGGGTGAC GCTACTTTTG AAAAAGCAAA GAATTTTCTT 
TATGATTTAG TTTCTAATTT GGATGTTGGA ATTAATAAGA TTCGCATTGG GCTTGTAACC 
TACAATGATG AGACAAATCC AGAATTTTTG CTAAACAGCT ATTCTTCGAA AACTGAAATC 
TTAGAAAGTA TTCAGAATAT GAAATATGTA GAAGGATATA CCTACACAGG GAGAGCCCTT 
GAGTATGTAA ATACAACCTA CTTTACACAG GCAGCAGGCA GTAGATTTGA AGAGAGCGTC 
GCTCAAATTC TTATCATTGT GACAGAAGGA GACTCTTCAG ATACCCTCAC TGAGCCTGCT 
AAAGAACTCA AATCAAGAGG CATATCTGTT TATGTGGTTG GGACAAACAT CAAGTACGAT 
AGACAGCTTC AGGAAGCATC AAGCAAGCCC GACGAAAAGT TCTTTTATCA ACTCGATGAT 
TTTGATGACT CTGAGAATGT CACTGAGCAG CTGTTAAAAA ACCTGTGCTT TAGCATAGAT 
TTGAATATTC AAGTATATTC AAAGCGCTAC GCTGATGTTG TATTTCTAGT CGATTCTTCT 
ACAAGCATGG GAACCATCTT TTTTCAAAAG ATGAAGGATT TCATCATACA CATAATAAAC 
CAGTTAAATG TTGGAATAAA TAAACACCGT ATAGGACTGG CACAGTACAG TGGTCTACCC 
CAAACTGAAT TTCTACTGAA TCATTATGAA ACCAAAGAGG AGATACTAAA GCACATTAAG 
GAAACATTCA CATACAGAGG TGGCCCCTTA AAAACAGGAC ATGCTTTGGA ATTTGTACGC 
TCAACATTTT TCATAGAAGA GGCTGGGAGT AGGATTAACT ACGGCAACCC CCAGTTCTTA 
GTGGTCATAA CATCATCAAA ATCAGAGGAT GCTGTTAGAA GACATGCCGA GGAACTCAAA 
AGTGTTGGGG TCACAACCAT CTCTGTTGGC ATTGGCAATT CTGATCGAAA AGAACTGGAA 
AAAATTGCTA CTGATCCTTT TGTTTTCCAG ACCACAGGTT TACAGCACAT TTCAAATTTA 
CAGCAGGATG TGGCCAATGT TATTATAGCA GAAGACATGC TACAGTTTTC CCCAGTATCA 
GTTGTACCAG CAGTTTGCTC CAGTGCTTCT GTAGCTGACA TTGTGTTTCT AATTGATGAA 
TCTAGCAGCA TTGGCCCCAT CAACTTTCAA CTAACCAGAG TCTTTCTACA CAAGGTTGTT 
AGTGCACTGG ATATTTCCTT GAGTAATGTT AGAGTGGGCT TAGTGCTTTA CAGTGATGAG 
CCAAGACTTG AACTTAAACT GAACACTTTT AATGAAAAAT ATGAAATTTT GGACTTCATA 
ACTAAGCTGC CATACAGAGG AGGCAAAGCA CATACAGGTG CTGCGCTGGA CTTTCTAAGG 
AAAAAAATGT TCACCAAACA AAATGGCGGC CGGCCACATC AAGGAGTTCA GCAGATAGCG 
GTGGTTATGA CTAATGGGCA GTCCATGGAT AATTTTACTA AACCTGCTGC AAAACTGAGA 
CGGTCTGGCG TGGAGGTTTT TGCTGTTGGC TTTCAGAATA TCAATGATAC AGAACTGGAT 
ATCATAGCAT CTCACCCACC GCGAAAACAT GTCACAAATG TGGAATCTTT CCTGCAGCTA 
TCCAACCTTG AGTTCAGAAT ACAGAAACGA CTTTGCAATG AAATTGTTGT GAAGTCATTT 
GCAGTACCTG CAATTGCTCG TGCAGTGAAG GAAGGGTGTG TGGATACAGA AGAAGCAGAT 
ATATACTTTC TAATTGATGG TTCTGGCAGT ATATATCCAG AGGACTTTGA AGATATGAAA 
AAATTCATGA TTGAATTAAT AAGCATGTTT CAAGTTGGTG CCAACAGAGT ACGCTTTGGA 
GTTGTCCAAT ACTCAGATGT TCGTAGAACT GAATTTTTCA TCAGTGAACA CAACACCCAG 
AAAATGTTAA AGGATGCTAT TTCTCAAATA GAACAGTTGG GCGGTGGTAC CCTAACAGGA 
GAAGCTTTAA CGTCAATGAA GCAACTTTTT GTTAATGCAG CTAAAGATCG CCCTCATAAA 
GTACCCCAGT CATTGGTTGT CATTACAGAT GGAGAATCTC AAGACCGGGT CACAGAAGCA 
GCTGCAGAAA TTAGAAATGA TGGCATTACT ATATTTGCAA TTGGTGTTAA AAATGCAGTG 
GAAGAAGAGA TTAGAGATAT AGCAGGGTCA AATGAGAAGA TGTTTTTTGT TAACAACTTT 
GATTCCCTGA AGGTGATCAA GAATGATTTG GCTCGTGAAT TGTGTACACC TGAGGCATGT 
AAGAACATGA AAGCTGATAT CGTGTTTCTG GTTGATAGCT CTGCAAGCAT TAACAGTGAT 
GATTATGAAA CAATGAAAGA ATTCATGGAG TCAATGGTCA AGCAGGCAGA AATCGGCCCT 
GATAGAGTCC AGATTGGTTT AATTCAGTTT AGCTCTGAAA CAAAGGAAGA ATTTCCTCTC 
AACAGGTACA AAAGAAAGGA TGAAATACAA TCAGCAATAA GAGGCATCCA ACAACTGAGC 
CAGGGAACCC TTATGGGGGA AGCCCTTAAA TATACATTAC CTTATTTTAG TGCTTCCAAA 
GGAGGCCGTG TAAATACCAA ACAATATCTG ATCGTCATAA CAGATGGTGA GGCCCAGGAT 
GCTGTGGGAA ACCCAGCCAA AGCAATAAGG GACCATGGAG TTATCATTTA TGCCATAGGA 
GTACAACAAG CCAACAACAC ACAACTTCTG GAGATTGCTG GCAAACAAGA GCAGGTGTAT 
TATGAAGATA GCTTTGATTC CCTAGCTTTT TTGAACAAGA ACATCATGTT TGAGATTTGC 
AATCCTCAAG AATCTTGCAA AAAGACTGAG GTGGCAGACA TCATATTTCT GTTGGATGCT 
TCTGCAAGCA TAACCAGAGG AGAGTTTAGA CTCATGCAGA GATTTGTGGA GGCTGTTGTC 
AATGACTCTT TGGTTGGAAA GGATAATGTT CAATTTGGAG CAGTTGTTTA CGGCACAAAT 
CCAGCAGAAC AGTTTTCACT CAATACGTAT TCAACTAAAC TTGATATTCT TAAGGCTGTA 
TTTAGTTTAC CCCAAGTATC TGGATACACA TACACTGCAA AAGCGCTGGA ATACACTCGG 
ATACGGTTTG GTACATCATA TGGTGGAAGA CCAGGAATTT CACATATTCT AATTCTAGTC 
ACAGATGGAG CCACTACTGA AGCTGATCGC CCTAACCTAC CAATAGTATC AAAGGCTTTG 
AAGGATGATG GCATCATTGT CTTTGCAGTA GGAGTTGGCA AGGCAGTTCC TCAAGAGCTT 
CAGCAGATTG CAGGATACCC AGACAGATGG TTCTTAGTCC AGAACTACAA AGGCCTGGAC 
AATATACATG ATAATATAAC ACAGGTTGTA TGTGATGAAT CAAAACCAGC TTGCTCACAT 
GAACAGCTTG ATCTTGTTTT CCTGATTGAC GGCTCAGCAA GTATCACTTC TAGCAACTTC 
ACAAGTGCCA AGACTTTCAT GAAAGAAATC GTTGACAGCT TTACAATTTC AGAGAACAGA 
GTGCGTATTG GAGTCGCACA GTACAGTGCA AATCCCAAGA AAGAATTCTT CTTAAATGAG 
TACTACTCCA GCTCCGACAT GAAGAAACAG ATTGATAGCA TCTCCCAACT GAAAGCTACC 
ACTTACACTG GCAAAGGCCT ACGATTTGTC AAACAGTTCT TTGATCCAGC AAATGGCGGC 
CGTAAGAATG TTCCGCAGTA CCTGATAGTC ATGACTGATG GCATGTCTAA TGACAGCGTA 
AATGAAGATG CTGCTGCTTT GCGAAGCAGT GGAGTAAAGA TCTTTTCCAT TGGTATTGGT 
CTGCGGAACA GTTTTGAGCT TGTTATGATT GCCGGGTCAC CCAAAAATGT TTACGAAGTG 
GAAACGTTCC AAGCGCTGGA CTCTATAAAG AGGCAGATTG TGGCACAAGT CTGTGAGCCA 
TCTGATCAGC CTATTATAGG TAAAGAATGC AGCTTAGATA TCACCATATC CGTTGACTAC 
TCAAGAAGAG CAATGCTATC TACTGTCCCC CGTGTGCAGC AGAAATTGCA GCAGTTCCTC 
CCTCCGGTGC TTCAGCGCAT TTCCTCGCTT AGCAATATCA GCTGTGCCTC TGGATCTCAA 
ATTAACTTAC GCTTCAGATA CGCTGTTGCA GCACAGGACA GACTGACATT TGATTCCGAC 
TTTGAGAAAT TTAACGAGGA CATCATTAAG AAGTTTATAG CTGAACAGTC TTCTGTTGAT 
ACATATTTAA ATGCAAAGTT TCTTCAGTCC CTCTGGAACA AATACATAGG TTTGCCGTCT 
CAGAAAGCAA AAATTATTCT GGTTTTTACT GATGGACTGG ATGAAAGTTC TGAAGTACTC 
AAGGCGACTA CTGAATCTCT TCGGAAACAA GGGTTGGATG CACTGTTATT CATTGCTCTT 
GAGGGGGTGA AGGATTTTAC AGAGCTGCAA AACTTAGAGT TTGGAAGAGG ATTTTCTTAC 
CAGCAACCTC TGAGCATTGC GCTGCATGAT CTGCCAAGTG TAATGCTAAA AGAAATGGAT 
ACCGTTGCAG AAAGGAAATG CTGTAATGTA GTTTGTAAAT GTTTAGGACA AGATGGACCT 
CTAGGCTCGC CAGGGGCTTT AGGAACAAAG GGTCAGCCTG GTATAAAGGG ATCTCAAGGT 
CATCCTGGAG AGGAAGGCGG TCATGGTGAA AGAGGATTCC GTGGGCTGAA TGGGACAAGA 
GGCGAAAGTG GATGTCCTGG TCGTAGGGGA TTAAAGGGTG CCAGAGGTAT TCGTGGTGAC 
AAGGGTGATG ATGGAGAGTT TGGAATTGAT GGTGTGCCTG GAGAGCAGGG TGAACCTGGA 
GGGCGTGGAG CATCAGGAGA ACGTGGAGAC ACTGGAGCTC AGGGCAGAAA AGGTCCTCGT 
GGGCAACCTG GAGAAAAAGG GGAAAATGGA CTGAGGGGAG ACCCAGGTGA GCCTGGAACT 
AACATTAACA TTCAGGGACC TAAAGGACTG AAAGGAAACC CAGGACGTCA GGGAGAGGCG 
GGAAGTGACG GTGTGGAAGG AGAGCCAGGA AATAATGGAC CTAATGGTCC ACAAGGCCGA 
AGAGGGCCTC CTGGTTTAAA GGGAGCACGA GGATTTCCTG GAGAGCCAGG GAACAAAGGT 
GATAGTGGAA TCCAAGGTAT ACAGGGTTCC CAAGGCATGC CTGGACCACC AGGTCCACAG 
GGACCACAAG GGTTATCTGG ACGTCAGGGA GATGCAGGTG CCCCTGGTGC ATCAGGTTCC 
TTTGGCAAAC CCGGAGCTCC AGGACTAAAG GGGGAACCTG GAGAAAACGG AGAGAAGGGT 
GAGCCAGGCA TTCCGGGTCG CAGAGGTTTG CCAGGGCTGG ATGGTCCTGA TGGATATGGT 
CCCCCAGGCT TAAAGGGAGA GAAGGGTCAT TTAGGGTTTC CTGGGGATCC TGGGCCTCAG 
GGAGAAGATG GTGATCCTGG AATAGCTGGA AATGGTGGCC CAAAAGGAAT TAGTGGAAGA 
AGGGGTAATT CTGGCCCTCC AGGAACTAAT GGACAAAGGG GTGAGAGGGG ACCACGTGGA 
CCTATGGGCG CCAAAGGATT GCCAGGAACT ATGCCTATGA CGCCTTGTGA ACTTGTGGCA 
TTTACTCGAG AGAATTGTCC TTGCTTCGCA AGTGCTTCAA AATGTCCAGT TTACCCAACT 
GAAATAGTGT TTGCTCTGGA TGCATCTCAG GACGTTCCCC CGGCTGCTTT CCAAAGGATG 
AAGAACACCA TTATTTCCAT AATAGAAAAG CTGGACATTA GTGAGAGCAA TTGCCCTAGA 
GGAGCCCGTG TTGCTGTTGT TGCGTATAAC AGTAATACCA AATACCTCAT TCGTTTCTCT 
GACTACAAAC GGAAAAGCCT ACTGCTGGAA GCAATTCGTG GCATCTCTCC AGAAAGATCA 
TCTTCAAGGA GAAATATTGG AGAAGCCATG AAGTTTGTAG GGAGGAACAT ATTCAAACGC 
ATTCGTCATG GAATCCATGT TAGAAAAGTG GCTGTGTTCT TTGCTAATGG CCCATCCCCA 
GATGCAGATG CAATAAACTC GGCAGTGCTG GAATACAGTG CTCTTGACAT TATTCCTATT 
GTGATTGCAT TTAATAATGT TCCCAATGTC GTAGCAGCTT TTTCGGCCGA CGAAACTAGA 
AGATTCCAAG TGTTTGTATG GAGTAGGCCT GAGGATCAGA GACTAGAACT TATTTCTTAT 
TGTACTCTTT GTTATGATAA GTGTACACCT GATGAAGAAT GTGAGCTGGA TCAAATGCCT 
GCAACCCTAA TAGATATGGA CATAGCTTTT GTATTAGACA GTTCACACAA TGTGCAAGCT 
GATGTCTTTG AGGAAATGAA AGACTTTGTG AGCAGTATGC TGGACACCGT CTACATATCA 
ACGCGGCCCG CAGTTTCTGA AACAGAAGCC AGAGTTGCCT TAGTACAACA GGCAGTTAAG 
GACTTTGGTC CAAATAGGAA TACTAGCCCA GTTAAAACTG AGTTCGACCT TCTTCGCTAC 
AACAGCGCAA ATATGATGAA GAGGCACATC AAACAATCTG TCAGGCAACT GAATGGTCCT 
TCTGCAGTTG GCAATTCTGT TCAGTGGGTC ATTGATAATA TCTTCTTAAA AACACCAATG 
CCAAGAAAGC ATAAAGTACT TTTCCTCATT CTTGCCAGCC CAACAAGCTA TTGGGACAGA 
GAAAAATTGA AAGAAGTCTC CAAAAAAGCT AAGTGTCAAG GATTTGCAAT ATTTGTACTG 
GGTTTTGGAA AAACGATAAG TGAGCTGGAA ATGACACAGC TGCCGAGCTT GCCACAAGAC 
CAACATTTGC TTCGCTTGAT AAGCATGTCA AAACCAGAAC TTGTCTATGC AAAGAAGTTC 
AGTCATGCCT TCTTCAATCT ACTCGACCGT GGCTTTAATC ACTACCCACC CGCAGTATTG 
CAAGCAGAGT GCCAAGGGCG TGGGGACTCA TCTTTTTCAG TTGTAAGAAG CTTCCCTGAC 
ACACGGTTGA CACAAGTTGA CACTGCTGCA GCAGAAGTTC CAGATGAGAC TATAGAAGTA 
GTGGAGGTCC TTGGTACACC TGCACCTGAA GAAATTGTGG GGAAAAAAAC AGAGAAAATA 
ATCATAAACA AAGTTGCAGA CAACTGCTTA TTGGATGTGA ACCATGGCAC AGCGTGCAGG 
GGCTATCAGC GCATGTGGTA TTATATAAAA TCTATTGATG CATGCTCCCA GTTCTGGTAT 
GGAGGTTGTG ATGGTAATGG AAACAGATTT AGCTCTGAAA CTGAGTGTCT GCAAACTTGC 
TCTTCCAAAA GTAAAAAAGC TAATGGGGAA GTGCGGAGCC TTAAGAAAGA TGTCTGTACA 
ATGAAACAAG AGGAAGGAAA ATGTGCAGAT TACATCCTCA AGTGGTCCTA CAACCAAGGC 
AAAAAAGCTT GTGTGCAGTT CTGGTACGGT GGCTGTGGAG GAAATGGCAA TCGTTTTGAC 
ACACAGGAAC AGTGTGAAGC CACCTGCATG TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820988.1
CDS	129..8444
Translation	MGFWKVFLACVLGSYSVNVSASQKTVCTEASIADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREPAIAVKNAGIILYAIGIKDAVLSELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEPAKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRRHAEELKSVGVTTISVGIGNSDRKELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKPAAKLRRSGVEVFAVGFQNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELCTPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNPAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNPQESCKKTEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVPQELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVNEDAAALRSSGVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQVCEPSDQPIIGKECSLDITISVDYSRRAMLSTVPRVQQKLQQFLPPVLQRISSLSNISCASGSQINLRFRYAVAAQDRLTFDSDFEKFNEDIIKKFIAEQSSVDTYLNAKFLQSLWNKYIGLPSQKAKIILVFTDGLDESSEVLKATTESLRKQGLDALLFIALEGVKDFTELQNLEFGRGFSYQQPLSIALHDLPSVMLKEMDTVAERKCCNVVCKCLGQDGPLGSPGALGTKGQPGIKGSQGHPGEEGGHGERGFRGLNGTRGESGCPGRRGLKGARGIRGDKGDDGEFGIDGVPGEQGEPGGRGASGERGDTGAQGRKGPRGQPGEKGENGLRGDPGEPGTNINIQGPKGLKGNPGRQGEAGSDGVEGEPGNNGPNGPQGRRGPPGLKGARGFPGEPGNKGDSGIQGIQGSQGMPGPPGPQGPQGLSGRQGDAGAPGASGSFGKPGAPGLKGEPGENGEKGEPGIPGRRGLPGLDGPDGYGPPGLKGEKGHLGFPGDPGPQGEDGDPGIAGNGGPKGISGRRGNSGPPGTNGQRGERGPRGPMGAKGLPGTMPMTPCELVAFTRENCPCFASASKCPVYPTEIVFALDASQDVPPAAFQRMKNTIISIIEKLDISESNCPRGARVAVVAYNSNTKYLIRFSDYKRKSLLLEAIRGISPERSSSRRNIGEAMKFVGRNIFKRIRHGIHVRKVAVFFANGPSPDADAINSAVLEYSALDIIPIVIAFNNVPNVVAAFSADETRRFQVFVWSRPEDQRLELISYCTLCYDKCTPDEECELDQMPATLIDMDIAFVLDSSHNVQADVFEEMKDFVSSMLDTVYISTRPAVSETEARVALVQQAVKDFGPNRNTSPVKTEFDLLRYNSANMMKRHIKQSVRQLNGPSAVGNSVQWVIDNIFLKTPMPRKHKVLFLILASPTSYWDREKLKEVSKKAKCQGFAIFVLGFGKTISELEMTQLPSLPQDQHLLRLISMSKPELVYAKKFSHAFFNLLDRGFNHYPPAVLQAECQGRGDSSFSVVRSFPDTRLTQVDTAAAEVPDETIEVVEVLGTPAPEEIVGKKTEKIIINKVADNCLLDVNHGTACRGYQRMWYYIKSIDACSQFWYGGCDGNGNRFSSETECLQTCSSKSKKANGEVRSLKKDVCTMKQEEGKCADYILKWSYNQGKKACVQFWYGGCGGNGNRFDTQEQCEATCMS

Target ORF information:

RefSeq Version	XM_012965534.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen alpha-6(VI) chain-like (LOC100486063), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965534.1

ORF Insert Sequence:

ATGGGATTTT GGAAAGTATT TCTCGCCTGC GTGCTTGGCA GCTACTCAGT CAATGTCTCA 
GCTTCTCAGA AAACAGTGTG CACAGAGGCC AGTATTGCAG ATATTGTATT CCTTATGGAT 
GGATCCTGGA GCATCGGAAC GGAAAACTTT ATCACAATGA AGAACTTTTT GTACACTTTG 
GTTAATGGTT TTGATGTTGG CCTAGATAAG ATCCAGATTG GCCTAATCCA GTACAGTGAT 
AATGCACGTA CAGAATTCTT CCTAAACAGT TACAGTAACA AAGAAGATGT TTTGAAATAT 
ATTCAGAATC TAAAGTATAA AGGGGGTGGG ACAAAGACTG GCTTAAGTCT GGAGTTCATG 
CTGACGCAGC ACTTTAGTGA AGCTGCAGGC AGCAGAGCAG CAGAAGGTGT GCCTCAGATT 
GCAGTCGTTA TAACTGATGG ACAAGCTCAA GATAGCATCA GGGAGCCTGC CATTGCAGTG 
AAGAACGCTG GAATCATACT GTATGCCATT GGCATTAAAG ATGCAGTTTT ATCTGAGCTT 
AATGAAATAG CCAGTGATCC AGATGACAAA CATGTTTATA GCGTTGCAGA CTTTAATGCA 
CTGCAGAGTA TTTCCCAAAA CATGATCCAG GTGCTCTGCA CAACTGTAGA AGAAGCAGCA 
AGACAGACAG GTCAAATTGC TCAAGTTTGC AGAACTGCCA ATCAAGCTGA CATTGTGCTG 
CTAGTCGAAA GCACAACAAG AATGGGTGAC GCTACTTTTG AAAAAGCAAA GAATTTTCTT 
TATGATTTAG TTTCTAATTT GGATGTTGGA ATTAATAAGA TTCGCATTGG GCTTGTAACC 
TACAATGATG AGACAAATCC AGAATTTTTG CTAAACAGCT ATTCTTCGAA AACTGAAATC 
TTAGAAAGTA TTCAGAATAT GAAATATGTA GAAGGATATA CCTACACAGG GAGAGCCCTT 
GAGTATGTAA ATACAACCTA CTTTACACAG GCAGCAGGCA GTAGATTTGA AGAGAGCGTC 
GCTCAAATTC TTATCATTGT GACAGAAGGA GACTCTTCAG ATACCCTCAC TGAGCCTGCT 
AAAGAACTCA AATCAAGAGG CATATCTGTT TATGTGGTTG GGACAAACAT CAAGTACGAT 
AGACAGCTTC AGGAAGCATC AAGCAAGCCC GACGAAAAGT TCTTTTATCA ACTCGATGAT 
TTTGATGACT CTGAGAATGT CACTGAGCAG CTGTTAAAAA ACCTGTGCTT TAGCATAGAT 
TTGAATATTC AAGTATATTC AAAGCGCTAC GCTGATGTTG TATTTCTAGT CGATTCTTCT 
ACAAGCATGG GAACCATCTT TTTTCAAAAG ATGAAGGATT TCATCATACA CATAATAAAC 
CAGTTAAATG TTGGAATAAA TAAACACCGT ATAGGACTGG CACAGTACAG TGGTCTACCC 
CAAACTGAAT TTCTACTGAA TCATTATGAA ACCAAAGAGG AGATACTAAA GCACATTAAG 
GAAACATTCA CATACAGAGG TGGCCCCTTA AAAACAGGAC ATGCTTTGGA ATTTGTACGC 
TCAACATTTT TCATAGAAGA GGCTGGGAGT AGGATTAACT ACGGCAACCC CCAGTTCTTA 
GTGGTCATAA CATCATCAAA ATCAGAGGAT GCTGTTAGAA GACATGCCGA GGAACTCAAA 
AGTGTTGGGG TCACAACCAT CTCTGTTGGC ATTGGCAATT CTGATCGAAA AGAACTGGAA 
AAAATTGCTA CTGATCCTTT TGTTTTCCAG ACCACAGGTT TACAGCACAT TTCAAATTTA 
CAGCAGGATG TGGCCAATGT TATTATAGCA GAAGACATGC TACAGTTTTC CCCAGTATCA 
GTTGTACCAG CAGTTTGCTC CAGTGCTTCT GTAGCTGACA TTGTGTTTCT AATTGATGAA 
TCTAGCAGCA TTGGCCCCAT CAACTTTCAA CTAACCAGAG TCTTTCTACA CAAGGTTGTT 
AGTGCACTGG ATATTTCCTT GAGTAATGTT AGAGTGGGCT TAGTGCTTTA CAGTGATGAG 
CCAAGACTTG AACTTAAACT GAACACTTTT AATGAAAAAT ATGAAATTTT GGACTTCATA 
ACTAAGCTGC CATACAGAGG AGGCAAAGCA CATACAGGTG CTGCGCTGGA CTTTCTAAGG 
AAAAAAATGT TCACCAAACA AAATGGCGGC CGGCCACATC AAGGAGTTCA GCAGATAGCG 
GTGGTTATGA CTAATGGGCA GTCCATGGAT AATTTTACTA AACCTGCTGC AAAACTGAGA 
CGGTCTGGCG TGGAGGTTTT TGCTGTTGGC TTTCAGAATA TCAATGATAC AGAACTGGAT 
ATCATAGCAT CTCACCCACC GCGAAAACAT GTCACAAATG TGGAATCTTT CCTGCAGCTA 
TCCAACCTTG AGTTCAGAAT ACAGAAACGA CTTTGCAATG AAATTGTTGT GAAGTCATTT 
GCAGTACCTG CAATTGCTCG TGCAGTGAAG GAAGGGTGTG TGGATACAGA AGAAGCAGAT 
ATATACTTTC TAATTGATGG TTCTGGCAGT ATATATCCAG AGGACTTTGA AGATATGAAA 
AAATTCATGA TTGAATTAAT AAGCATGTTT CAAGTTGGTG CCAACAGAGT ACGCTTTGGA 
GTTGTCCAAT ACTCAGATGT TCGTAGAACT GAATTTTTCA TCAGTGAACA CAACACCCAG 
AAAATGTTAA AGGATGCTAT TTCTCAAATA GAACAGTTGG GCGGTGGTAC CCTAACAGGA 
GAAGCTTTAA CGTCAATGAA GCAACTTTTT GTTAATGCAG CTAAAGATCG CCCTCATAAA 
GTACCCCAGT CATTGGTTGT CATTACAGAT GGAGAATCTC AAGACCGGGT CACAGAAGCA 
GCTGCAGAAA TTAGAAATGA TGGCATTACT ATATTTGCAA TTGGTGTTAA AAATGCAGTG 
GAAGAAGAGA TTAGAGATAT AGCAGGGTCA AATGAGAAGA TGTTTTTTGT TAACAACTTT 
GATTCCCTGA AGGTGATCAA GAATGATTTG GCTCGTGAAT TGTGTACACC TGAGGCATGT 
AAGAACATGA AAGCTGATAT CGTGTTTCTG GTTGATAGCT CTGCAAGCAT TAACAGTGAT 
GATTATGAAA CAATGAAAGA ATTCATGGAG TCAATGGTCA AGCAGGCAGA AATCGGCCCT 
GATAGAGTCC AGATTGGTTT AATTCAGTTT AGCTCTGAAA CAAAGGAAGA ATTTCCTCTC 
AACAGGTACA AAAGAAAGGA TGAAATACAA TCAGCAATAA GAGGCATCCA ACAACTGAGC 
CAGGGAACCC TTATGGGGGA AGCCCTTAAA TATACATTAC CTTATTTTAG TGCTTCCAAA 
GGAGGCCGTG TAAATACCAA ACAATATCTG ATCGTCATAA CAGATGGTGA GGCCCAGGAT 
GCTGTGGGAA ACCCAGCCAA AGCAATAAGG GACCATGGAG TTATCATTTA TGCCATAGGA 
GTACAACAAG CCAACAACAC ACAACTTCTG GAGATTGCTG GCAAACAAGA GCAGGTGTAT 
TATGAAGATA GCTTTGATTC CCTAGCTTTT TTGAACAAGA ACATCATGTT TGAGATTTGC 
AATCCTCAAG AATCTTGCAA AAAGACTGAG GTGGCAGACA TCATATTTCT GTTGGATGCT 
TCTGCAAGCA TAACCAGAGG AGAGTTTAGA CTCATGCAGA GATTTGTGGA GGCTGTTGTC 
AATGACTCTT TGGTTGGAAA GGATAATGTT CAATTTGGAG CAGTTGTTTA CGGCACAAAT 
CCAGCAGAAC AGTTTTCACT CAATACGTAT TCAACTAAAC TTGATATTCT TAAGGCTGTA 
TTTAGTTTAC CCCAAGTATC TGGATACACA TACACTGCAA AAGCGCTGGA ATACACTCGG 
ATACGGTTTG GTACATCATA TGGTGGAAGA CCAGGAATTT CACATATTCT AATTCTAGTC 
ACAGATGGAG CCACTACTGA AGCTGATCGC CCTAACCTAC CAATAGTATC AAAGGCTTTG 
AAGGATGATG GCATCATTGT CTTTGCAGTA GGAGTTGGCA AGGCAGTTCC TCAAGAGCTT 
CAGCAGATTG CAGGATACCC AGACAGATGG TTCTTAGTCC AGAACTACAA AGGCCTGGAC 
AATATACATG ATAATATAAC ACAGGTTGTA TGTGATGAAT CAAAACCAGC TTGCTCACAT 
GAACAGCTTG ATCTTGTTTT CCTGATTGAC GGCTCAGCAA GTATCACTTC TAGCAACTTC 
ACAAGTGCCA AGACTTTCAT GAAAGAAATC GTTGACAGCT TTACAATTTC AGAGAACAGA 
GTGCGTATTG GAGTCGCACA GTACAGTGCA AATCCCAAGA AAGAATTCTT CTTAAATGAG 
TACTACTCCA GCTCCGACAT GAAGAAACAG ATTGATAGCA TCTCCCAACT GAAAGCTACC 
ACTTACACTG GCAAAGGCCT ACGATTTGTC AAACAGTTCT TTGATCCAGC AAATGGCGGC 
CGTAAGAATG TTCCGCAGTA CCTGATAGTC ATGACTGATG GCATGTCTAA TGACAGCGTA 
AATGAAGATG CTGCTGCTTT GCGAAGCAGT GGAGTAAAGA TCTTTTCCAT TGGTATTGGT 
CTGCGGAACA GTTTTGAGCT TGTTATGATT GCCGGGTCAC CCAAAAATGT TTACGAAGTG 
GAAACGTTCC AAGCGCTGGA CTCTATAAAG AGGCAGATTG TGGCACAAGT CTGTGAGCCA 
TCTGATCAGC CTATTATAGG TAAAGAATGC AGCTTAGATA TCACCATATC CGTTGACTAC 
TCAAGAAGAG CAATGCTATC TACTGTCCCC CGTGTGCAGC AGAAATTGCA GCAGTTCCTC 
CCTCCGGTGC TTCAGCGCAT TTCCTCGCTT AGCAATATCA GCTGTGCCTC TGGATCTCAA 
ATTAACTTAC GCTTCAGATA CGCTGTTGCA GCACAGGACA GACTGACATT TGATTCCGAC 
TTTGAGAAAT TTAACGAGGA CATCATTAAG AAGTTTATAG CTGAACAGTC TTCTGTTGAT 
ACATATTTAA ATGCAAAGTT TCTTCAGTCC CTCTGGAACA AATACATAGG TTTGCCGTCT 
CAGAAAGCAA AAATTATTCT GGTTTTTACT GATGGACTGG ATGAAAGTTC TGAAGTACTC 
AAGGCGACTA CTGAATCTCT TCGGAAACAA GGGTTGGATG CACTGTTATT CATTGCTCTT 
GAGGGGGTGA AGGATTTTAC AGAGCTGCAA AACTTAGAGT TTGGAAGAGG ATTTTCTTAC 
CAGCAACCTC TGAGCATTGC GCTGCATGAT CTGCCAAGTG TAATGCTAAA AGAAATGGAT 
ACCGTTGCAG AAAGGAAATG CTGTAATGTA GTTTGTAAAT GTTTAGGACA AGATGGACCT 
CTAGGCTCGC CAGGGGCTTT AGGAACAAAG GGTCAGCCTG GTATAAAGGG ATCTCAAGGT 
CATCCTGGAG AGGAAGGCGG TCATGGTGAA AGAGGATTCC GTGGGCTGAA TGGGACAAGA 
GGCGAAAGTG GATGTCCTGG TCGTAGGGGA TTAAAGGGTG CCAGAGGTAT TCGTGGTGAC 
AAGGGTGATG ATGGAGAGTT TGGAATTGAT GGTGTGCCTG GAGAGCAGGG TGAACCTGGA 
GGGCGTGGAG CATCAGGAGA ACGTGGAGAC ACTGGAGCTC AGGGCAGAAA AGGTCCTCGT 
GGGCAACCTG GAGAAAAAGG GGAAAATGGA CTGAGGGGAG ACCCAGGTGA GCCTGGAACT 
AACATTAACA TTCAGGGACC TAAAGGACTG AAAGGAAACC CAGGACGTCA GGGAGAGGCG 
GGAAGTGACG GTGTGGAAGG AGAGCCAGGA AATAATGGAC CTAATGGTCC ACAAGGCCGA 
AGAGGGCCTC CTGGTTTAAA GGGAGCACGA GGATTTCCTG GAGAGCCAGG GAACAAAGGT 
GATAGTGGAA TCCAAGGTAT ACAGGGTTCC CAAGGCATGC CTGGACCACC AGGTCCACAG 
GGACCACAAG GGTTATCTGG ACGTCAGGGA GATGCAGGTG CCCCTGGTGC ATCAGGTTCC 
TTTGGCAAAC CCGGAGCTCC AGGACTAAAG GGGGAACCTG GAGAAAACGG AGAGAAGGGT 
GAGCCAGGCA TTCCGGGTCG CAGAGGTTTG CCAGGGCTGG ATGGTCCTGA TGGATATGGT 
CCCCCAGGCT TAAAGGGAGA GAAGGGTCAT TTAGGGTTTC CTGGGGATCC TGGGCCTCAG 
GGAGAAGATG GTGATCCTGG AATAGCTGGA AATGGTGGCC CAAAAGGAAT TAGTGGAAGA 
AGGGGTAATT CTGGCCCTCC AGGAACTAAT GGACAAAGGG GTGAGAGGGG ACCACGTGGA 
CCTATGGGCG CCAAAGGATT GCCAGGAACT ATGCCTATGA CGCCTTGTGA ACTTGTGGCA 
TTTACTCGAG AGAATTGTCC TTGCTTCGCA AGTGCTTCAA AATGTCCAGT TTACCCAACT 
GAAATAGTGT TTGCTCTGGA TGCATCTCAG GACGTTCCCC CGGCTGCTTT CCAAAGGATG 
AAGAACACCA TTATTTCCAT AATAGAAAAG CTGGACATTA GTGAGAGCAA TTGCCCTAGA 
GGAGCCCGTG TTGCTGTTGT TGCGTATAAC AGTAATACCA AATACCTCAT TCGTTTCTCT 
GACTACAAAC GGAAAAGCCT ACTGCTGGAA GCAATTCGTG GCATCTCTCC AGAAAGATCA 
TCTTCAAGGA GAAATATTGG AGAAGCCATG AAGTTTGTAG GGAGGAACAT ATTCAAACGC 
ATTCGTCATG GAATCCATGT TAGAAAAGTG GCTGTGTTCT TTGCTAATGG CCCATCCCCA 
GATGCAGATG CAATAAACTC GGCAGTGCTG GAATACAGTG CTCTTGACAT TATTCCTATT 
GTGATTGCAT TTAATAATGT TCCCAATGTC GTAGCAGCTT TTTCGGCCGA CGAAACTAGA 
AGATTCCAAG TGTTTGTATG GAGTAGGCCT GAGGATCAGA GACTAGAACT TATTTCTTAT 
TGTACTCTTT GTTATGATAA GTGTACACCT GATGAAGAAT GTGAGCTGGA TCAAATGCCT 
GCAACCCTAA TAGATATGGA CATAGCTTTT GTATTAGACA GTTCACACAA TGTGCAAGCT 
GATGTCTTTG AGGAAATGAA AGACTTTGTG AGCAGTATGC TGGACACCGT CTACATATCA 
ACGCGGCCCG CAGTTTCTGA AACAGAAGCC AGAGTTGCCT TAGTACAACA GGCAGTTAAG 
GACTTTGGTC CAAATAGGAA TACTAGCCCA GTTAAAACTG AGTTCGACCT TCTTCGCTAC 
AACAGCGCAA ATATGATGAA GAGGCACATC AAACAATCTG TCAGGCAACT GAATGGTCCT 
TCTGCAGTTG GCAATTCTGT TCAGTGGGTC ATTGATAATA TCTTCTTAAA AACACCAATG 
CCAAGAAAGC ATAAAGTACT TTTCCTCATT CTTGCCAGCC CAACAAGCTA TTGGGACAGA 
GAAAAATTGA AAGAAGTCTC CAAAAAAGCT AAGTGTCAAG GATTTGCAAT ATTTGTACTG 
GGTTTTGGAA AAACGATAAG TGAGCTGGAA ATGACACAGC TGCCGAGCTT GCCACAAGAC 
CAACATTTGC TTCGCTTGAT AAGCATGTCA AAACCAGAAC TTGTCTATGC AAAGAAGTTC 
AGTCATGCCT TCTTCAATCT ACTCGACCGT GGCTTTAATC ACTACCCACC CGCAGTATTG 
CAAGCAGAGT GCCAAGGGCG TGGGGACTCA TCTTTTTCAG TTGTAAGAAG CTTCCCTGAC 
ACACGGTTGA CACAAGTTGA CACTGCTGCA GCAGAAGTTC CAGATGAGAC TATAGAAGTA 
GTGGAGGTCC TTGGTACACC TGCACCTGAA GAAATTGTGG GGAAAAAAAC AGAGAAAATA 
ATCATAAACA AAGTTGCAGA CAACTGCTTA TTGGATGTGA ACCATGGCAC AGCGTGCAGG 
GGCTATCAGC GCATGTGGTA TTATATAAAA TCTATTGATG CATGCTCCCA GTTCTGGTAT 
GGAGGTTGTG ATGGTAATGG AAACAGATTT AGCTCTGAAA CTGAGTGTCT GCAAACTTGC 
TCTTCCAAAA GTAAAAAAGC TAATGGGGAA GTGCGGAGCC TTAAGAAAGA TGTCTGTACA 
ATGAAACAAG AGGAAGGAAA ATGTGCAGAT TACATCCTCA AGTGGTCCTA CAACCAAGGC 
AAAAAAGCTT GTGTGCAGTT CTGGTACGGT GGCTGTGGAG GAAATGGCAA TCGTTTTGAC 
ACACAGGAAC AGTGTGAAGC CACCTGCATG TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col6a5 ( XM_012965535.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820989.1 Xenopus tropicalis collagen alpha-6(VI) chain-like (LOC100486063), transcript variant X2, mRNA.

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CloneID	OXa33518
Clone ID Related Accession (Same CDS sequence)	XM_012965535.1
Accession Version	XM_012965535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-6(VI) chain-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTTT GGAAAGTATT TCTCGCCTGC GTGCTTGGCA GCTACTCAGT CAATGTCTCA 
GCTTCTCAGA AAACAGTGTG CACAGAGGCC AGTATTGCAG ATATTGTATT CCTTATGGAT 
GGATCCTGGA GCATCGGAAC GGAAAACTTT ATCACAATGA AGAACTTTTT GTACACTTTG 
GTTAATGGTT TTGATGTTGG CCTAGATAAG ATCCAGATTG GCCTAATCCA GTACAGTGAT 
AATGCACGTA CAGAATTCTT CCTAAACAGT TACAGTAACA AAGAAGATGT TTTGAAATAT 
ATTCAGAATC TAAAGTATAA AGGGGGTGGG ACAAAGACTG GCTTAAGTCT GGAGTTCATG 
CTGACGCAGC ACTTTAGTGA AGCTGCAGGC AGCAGAGCAG CAGAAGGTGT GCCTCAGATT 
GCAGTCGTTA TAACTGATGG ACAAGCTCAA GATAGCATCA GGGAGCCTGC CATTGCAGTG 
AAGAACGCTG GAATCATACT GTATGCCATT GGCATTAAAG ATGCAGTTTT ATCTGAGCTT 
AATGAAATAG CCAGTGATCC AGATGACAAA CATGTTTATA GCGTTGCAGA CTTTAATGCA 
CTGCAGAGTA TTTCCCAAAA CATGATCCAG GTGCTCTGCA CAACTGTAGA AGAAGCAGCA 
AGACAGACAG GTCAAATTGC TCAAGTTTGC AGAACTGCCA ATCAAGCTGA CATTGTGCTG 
CTAGTCGAAA GCACAACAAG AATGGGTGAC GCTACTTTTG AAAAAGCAAA GAATTTTCTT 
TATGATTTAG TTTCTAATTT GGATGTTGGA ATTAATAAGA TTCGCATTGG GCTTGTAACC 
TACAATGATG AGACAAATCC AGAATTTTTG CTAAACAGCT ATTCTTCGAA AACTGAAATC 
TTAGAAAGTA TTCAGAATAT GAAATATGTA GAAGGATATA CCTACACAGG GAGAGCCCTT 
GAGTATGTAA ATACAACCTA CTTTACACAG GCAGCAGGCA GTAGATTTGA AGAGAGCGTC 
GCTCAAATTC TTATCATTGT GACAGAAGGA GACTCTTCAG ATACCCTCAC TGAGCCTGCT 
AAAGAACTCA AATCAAGAGG CATATCTGTT TATGTGGTTG GGACAAACAT CAAGTACGAT 
AGACAGCTTC AGGAAGCATC AAGCAAGCCC GACGAAAAGT TCTTTTATCA ACTCGATGAT 
TTTGATGACT CTGAGAATGT CACTGAGCAG CTGTTAAAAA ACCTGTGCTT TAGCATAGAT 
TTGAATATTC AAGTATATTC AAAGCGCTAC GCTGATGTTG TATTTCTAGT CGATTCTTCT 
ACAAGCATGG GAACCATCTT TTTTCAAAAG ATGAAGGATT TCATCATACA CATAATAAAC 
CAGTTAAATG TTGGAATAAA TAAACACCGT ATAGGACTGG CACAGTACAG TGGTCTACCC 
CAAACTGAAT TTCTACTGAA TCATTATGAA ACCAAAGAGG AGATACTAAA GCACATTAAG 
GAAACATTCA CATACAGAGG TGGCCCCTTA AAAACAGGAC ATGCTTTGGA ATTTGTACGC 
TCAACATTTT TCATAGAAGA GGCTGGGAGT AGGATTAACT ACGGCAACCC CCAGTTCTTA 
GTGGTCATAA CATCATCAAA ATCAGAGGAT GCTGTTAGAA GACATGCCGA GGAACTCAAA 
AGTGTTGGGG TCACAACCAT CTCTGTTGGC ATTGGCAATT CTGATCGAAA AGAACTGGAA 
AAAATTGCTA CTGATCCTTT TGTTTTCCAG ACCACAGGTT TACAGCACAT TTCAAATTTA 
CAGCAGGATG TGGCCAATGT TATTATAGCA GAAGACATGC TACAGTTTTC CCCAGTATCA 
GTTGTACCAG CAGTTTGCTC CAGTGCTTCT GTAGCTGACA TTGTGTTTCT AATTGATGAA 
TCTAGCAGCA TTGGCCCCAT CAACTTTCAA CTAACCAGAG TCTTTCTACA CAAGGTTGTT 
AGTGCACTGG ATATTTCCTT GAGTAATGTT AGAGTGGGCT TAGTGCTTTA CAGTGATGAG 
CCAAGACTTG AACTTAAACT GAACACTTTT AATGAAAAAT ATGAAATTTT GGACTTCATA 
ACTAAGCTGC CATACAGAGG AGGCAAAGCA CATACAGGTG CTGCGCTGGA CTTTCTAAGG 
AAAAAAATGT TCACCAAACA AAATGGCGGC CGGCCACATC AAGGAGTTCA GCAGATAGCG 
GTGGTTATGA CTAATGGGCA GTCCATGGAT AATTTTACTA AACCTGCTGC AAAACTGAGA 
CGGTCTGGCG TGGAGGTTTT TGCTGTTGGC TTTCAGAATA TCAATGATAC AGAACTGGAT 
ATCATAGCAT CTCACCCACC GCGAAAACAT GTCACAAATG TGGAATCTTT CCTGCAGCTA 
TCCAACCTTG AGTTCAGAAT ACAGAAACGA CTTTGCAATG AAATTGTTGT GAAGTCATTT 
GCAGTACCTG CAATTGCTCG TGCAGTGAAG GAAGGGTGTG TGGATACAGA AGAAGCAGAT 
ATATACTTTC TAATTGATGG TTCTGGCAGT ATATATCCAG AGGACTTTGA AGATATGAAA 
AAATTCATGA TTGAATTAAT AAGCATGTTT CAAGTTGGTG CCAACAGAGT ACGCTTTGGA 
GTTGTCCAAT ACTCAGATGT TCGTAGAACT GAATTTTTCA TCAGTGAACA CAACACCCAG 
AAAATGTTAA AGGATGCTAT TTCTCAAATA GAACAGTTGG GCGGTGGTAC CCTAACAGGA 
GAAGCTTTAA CGTCAATGAA GCAACTTTTT GTTAATGCAG CTAAAGATCG CCCTCATAAA 
GTACCCCAGT CATTGGTTGT CATTACAGAT GGAGAATCTC AAGACCGGGT CACAGAAGCA 
GCTGCAGAAA TTAGAAATGA TGGCATTACT ATATTTGCAA TTGGTGTTAA AAATGCAGTG 
GAAGAAGAGA TTAGAGATAT AGCAGGGTCA AATGAGAAGA TGTTTTTTGT TAACAACTTT 
GATTCCCTGA AGGTGATCAA GAATGATTTG GCTCGTGAAT TGTGTACACC TGAGGCATGT 
AAGAACATGA AAGCTGATAT CGTGTTTCTG GTTGATAGCT CTGCAAGCAT TAACAGTGAT 
GATTATGAAA CAATGAAAGA ATTCATGGAG TCAATGGTCA AGCAGGCAGA AATCGGCCCT 
GATAGAGTCC AGATTGGTTT AATTCAGTTT AGCTCTGAAA CAAAGGAAGA ATTTCCTCTC 
AACAGGTACA AAAGAAAGGA TGAAATACAA TCAGCAATAA GAGGCATCCA ACAACTGAGC 
CAGGGAACCC TTATGGGGGA AGCCCTTAAA TATACATTAC CTTATTTTAG TGCTTCCAAA 
GGAGGCCGTG TAAATACCAA ACAATATCTG ATCGTCATAA CAGATGGTGA GGCCCAGGAT 
GCTGTGGGAA ACCCAGCCAA AGCAATAAGG GACCATGGAG TTATCATTTA TGCCATAGGA 
GTACAACAAG CCAACAACAC ACAACTTCTG GAGATTGCTG GCAAACAAGA GCAGGTGTAT 
TATGAAGATA GCTTTGATTC CCTAGCTTTT TTGAACAAGA ACATCATGTT TGAGATTTGC 
AATCCTCAAG AATCTTGCAA AAAGACTGAG GTGGCAGACA TCATATTTCT GTTGGATGCT 
TCTGCAAGCA TAACCAGAGG AGAGTTTAGA CTCATGCAGA GATTTGTGGA GGCTGTTGTC 
AATGACTCTT TGGTTGGAAA GGATAATGTT CAATTTGGAG CAGTTGTTTA CGGCACAAAT 
CCAGCAGAAC AGTTTTCACT CAATACGTAT TCAACTAAAC TTGATATTCT TAAGGCTGTA 
TTTAGTTTAC CCCAAGTATC TGGATACACA TACACTGCAA AAGCGCTGGA ATACACTCGG 
ATACGGTTTG GTACATCATA TGGTGGAAGA CCAGGAATTT CACATATTCT AATTCTAGTC 
ACAGATGGAG CCACTACTGA AGCTGATCGC CCTAACCTAC CAATAGTATC AAAGGCTTTG 
AAGGATGATG GCATCATTGT CTTTGCAGTA GGAGTTGGCA AGGCAGTTCC TCAAGAGCTT 
CAGCAGATTG CAGGATACCC AGACAGATGG TTCTTAGTCC AGAACTACAA AGGCCTGGAC 
AATATACATG ATAATATAAC ACAGGTTGTA TGTGATGAAT CAAAACCAGC TTGCTCACAT 
GAACAGCTTG ATCTTGTTTT CCTGATTGAC GGCTCAGCAA GTATCACTTC TAGCAACTTC 
ACAAGTGCCA AGACTTTCAT GAAAGAAATC GTTGACAGCT TTACAATTTC AGAGAACAGA 
GTGCGTATTG GAGTCGCACA GTACAGTGCA AATCCCAAGA AAGAATTCTT CTTAAATGAG 
TACTACTCCA GCTCCGACAT GAAGAAACAG ATTGATAGCA TCTCCCAACT GAAAGCTACC 
ACTTACACTG GCAAAGGCCT ACGATTTGTC AAACAGTTCT TTGATCCAGC AAATGGCGGC 
CGTAAGAATG TTCCGCAGTA CCTGATAGTC ATGACTGATG GCATGTCTAA TGACAGCGTA 
AATGAAGATG CTGCTGCTTT GCGAAGCAGT GGAGTAAAGA TCTTTTCCAT TGGTATTGGT 
CTGCGGAACA GTTTTGAGCT TGTTATGATT GCCGGGTCAC CCAAAAATGT TTACGAAGTG 
GAAACGTTCC AAGCGCTGGA CTCTATAAAG AGGCAGATTG TGGCACAAGT CTGTGAGCCA 
TCTGATCAGC CTATTATAGG TAAAGAATGC AGCTTAGATA TCACCATATC CGTTGACTAC 
TCAAGAAGAG CAATGCTATC TACTGTCCCC CGTGTGCAGC AGAAATTGCA GCAGTTCCTC 
CCTCCGGTGC TTCAGCGCAT TTCCTCGCTT AGCAATATCA GCTGTGCCTC TGGATCTCAA 
ATTAACTTAC GCTTCAGATA CGCTGTTGCA GCACAGGACA GACTGACATT TGATTCCGAC 
TTTGAGAAAT TTAACGAGGA CATCATTAAG AAGTTTATAG CTGAACAGTC TTCTGTTGAT 
ACATATTTAA ATGCAAAGTT TCTTCAGTCC CTCTGGAACA AATACATAGG TTTGCCGTCT 
CAGAAAGCAA AAATTATTCT GGTTTTTACT GATGGACTGG ATGAAAGTTC TGAAGTACTC 
AAGGCGACTA CTGAATCTCT TCGGAAACAA GGGTTGGATG CACTGTTATT CATTGCTCTT 
GAGGGGGTGA AGGATTTTAC AGAGCTGCAA AACTTAGAGT TTGGAAGAGG ATTTTCTTAC 
CAGCAACCTC TGAGCATTGC GCTGCATGAT CTGCCAAGTG TAATGCTAAA AGAAATGGAT 
ACCGTTGCAG AAAGGAAATG CTGTAATGTA GTTTGTAAAT GTTTAGGACA AGATGGACCT 
CTAGGCTCGC CAGGGGCTTT AGGAACAAAG GGTCAGCCTG GTATAAAGGG ATCTCAAGGT 
CATCCTGGAG AGGAAGGCGG TCATGGTGAA AGAGGATTCC GTGGGCTGAA TGGGACAAGA 
GGCGAAAGTG GATGTCCTGG TCGTAGGGGA TTAAAGGGTG CCAGAGGTAT TCGTGGTGAC 
AAGGGTGATG ATGGAGAGTT TGGAATTGAT GGTGTGCCTG GAGAGCAGGG TGAACCTGGA 
GGGCGTGGAG CATCAGGAGA ACGTGGAGAC ACTGGAGCTC AGGGCAGAAA AGGTCCTCGT 
GGGCAACCTG GAGAAAAAGG GGAAAATGGA CTGAGGGGAG ACCCAGGTGA GCCTGGAACT 
AACATTAACA TTCAGGGACC TAAAGGACTG AAAGGAAACC CAGGACGTCA GGGAGAGGCG 
GGAAGTGACG GTGTGGAAGG AGAGCCAGGA AATAATGGAC CTAATGGTCC ACAAGGCCGA 
AGAGGGCCTC CTGGTTTAAA GGGAGCACGA GGATTTCCTG GAGAGCCAGG GAACAAAGGT 
GATAGTGGAA TCCAAGGTAT ACAGGGTTCC CAAGGCATGC CTGGACCACC AGGTCCACAG 
GGACCACAAG GGTTATCTGG ACGTCAGGGA GATGCAGGTG CCCCTGGTGC ATCAGGTTCC 
TTTGGCAAAC CCGGAGCTCC AGGACTAAAG GGGGAACCTG GAGAAAACGG AGAGAAGGGT 
GAGCCAGGCA TTCCGGGTCG CAGAGGTTTG CCAGGGCTGG ATGGTCCTGA TGGATATGGT 
CCCCCAGGCT TAAAGGGAGA GAAGGGTCAT TTAGGGTTTC CTGGGGATCC TGGGCCTCAG 
GGAGAAGATG GTGATCCTGG AATAGCTGGA AATGGTGGCC CAAAAGGAAT TAGTGGAAGA 
AGGGGTAATT CTGGCCCTCC AGGAACTAAT GGACAAAGGG GTGAGAGGGG ACCACGTGGA 
CCTATGGGCG CCAAAGGATT GCCAGGAACT ATGCCTATGA CGCCTTGTGA ACTTGTGGCA 
TTTACTCGAG AGAATTGTCC TTGCTTCGCA AGTGCTTCAA AATGTCCAGT TTACCCAACT 
GAAATAGTGT TTGCTCTGGA TGCATCTCAG GACGTTCCCC CGGCTGCTTT CCAAAGGATG 
AAGAACACCA TTATTTCCAT AATAGAAAAG CTGGACATTA GTGAGAGCAA TTGCCCTAGA 
GGAGCCCGTG TTGCTGTTGT TGCGTATAAC AGTAATACCA AATACCTCAT TCGTTTCTCT 
GACTACAAAC GGAAAAGCCT ACTGCTGGAA GCAATTCGTG GCATCTCTCC AGAAAGATCA 
TCTTCAAGGA GAAATATTGG AGAAGCCATG AAGTTTGTAG GGAGGAACAT ATTCAAACGC 
ATTCGTCATG GAATCCATGT TAGAAAAGTG GCTGTGTTCT TTGCTAATGG CCCATCCCCA 
GATGCAGATG CAATAAACTC GGCAGTGCTG GAATACAGTG CTCTTGACAT TATTCCTATT 
GTGATTGCAT TTAATAATGT TCCCAATGTC GTAGCAGCTT TTTCGGCCGA CGAAACTAGA 
AGATTCCAAG TGTTTGTATG GAGTAGGCCT GAGGATCAGA GACTAGAACT TATTTCTTAT 
TGTACTCTTT GTTATGATAA GTGTACACCT GATGAAGAAT GTGAGCTGGA TCAAATGCCT 
GCAACCCTAA TAGATATGGA CATAGCTTTT GTATTAGACA GTTCACACAA TGTGCAAGCT 
GATGTCTTTG AGGAAATGAA AGACTTTGTG AGCAGTATGC TGGACACCGT CTACATATCA 
ACGCGGCCCG CAGTTTCTGA AACAGAAGCC AGAGTTGCCT TAGTACAACA GGCAGTTAAG 
GACTTTGGTC CAAATAGGAA TACTAGCCCA GTTAAAACTG AGTTCGACCT TCTTCGCTAC 
AACAGCGCAA ATATGATGAA GAGGCACATC AAACAATCTG TCAGGCAACT GAATGGTCCT 
TCTGCAGTTG GCAATTCTGT TCAGTGGGTC ATTGATAATA TCTTCTTAAA AACACCAATG 
CCAAGAAAGC ATAAAGTACT TTTCCTCATT CTTGCCAGCC CAACAAGCTA TTGGGACAGA 
GAAAAATTGA AAGAAGTCTC CAAAAAAGCT AAGTGTCAAG GATTTGCAAT ATTTGTACTG 
GGTTTTGGAA AAACGATAAG TGAGCTGGAA ATGACACAGC TGCCGAGCTT GCCACAAGAC 
CAACATTTGC TTCGCTTGAT AAGCATGTCA AAACCAGAAC TTGTCTATGC AAAGAAGTTC 
AGTCATGCCT TCTTCAATCT ACTCGACCGT GGCTTTAATC ACTACCCACC CGCAGTATTG 
CAAGCAGAGT GCCAAGGGCG TGGGGACTCA TCTTTTTCAG TTGTAAGAAG CTTCCCTGAC 
ACACGGTTGA CACAAGTTGA CACTGCTGCA GCAGAAGTTC CAGATGAGAC TATAGAAGTA 
GTGGAGGTCC TTGGTACACC TGCACCTGAA GAAATTGTGG GGAAAAAAAC AGAGAAAATA 
ATCATAAACA AAGGTAAAAA AGCTAATGGG GAAGTGCGGA GCCTTAAGAA AGATGTCTGT 
ACAATGAAAC AAGAGGAAGG AAAATGTGCA GATTACATCC TCAAGTGGTC CTACAACCAA 
GGCAAAAAAG CTTGTGTGCA GTTCTGGTAC GGTGGCTGTG GAGGAAATGG CAATCGTTTT 
GACACACAGG AACAGTGTGA AGCCACCTGC ATGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820989.1
CDS	129..8267
Translation	MGFWKVFLACVLGSYSVNVSASQKTVCTEASIADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREPAIAVKNAGIILYAIGIKDAVLSELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEPAKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRRHAEELKSVGVTTISVGIGNSDRKELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKPAAKLRRSGVEVFAVGFQNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELCTPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNPAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNPQESCKKTEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVPQELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVNEDAAALRSSGVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQVCEPSDQPIIGKECSLDITISVDYSRRAMLSTVPRVQQKLQQFLPPVLQRISSLSNISCASGSQINLRFRYAVAAQDRLTFDSDFEKFNEDIIKKFIAEQSSVDTYLNAKFLQSLWNKYIGLPSQKAKIILVFTDGLDESSEVLKATTESLRKQGLDALLFIALEGVKDFTELQNLEFGRGFSYQQPLSIALHDLPSVMLKEMDTVAERKCCNVVCKCLGQDGPLGSPGALGTKGQPGIKGSQGHPGEEGGHGERGFRGLNGTRGESGCPGRRGLKGARGIRGDKGDDGEFGIDGVPGEQGEPGGRGASGERGDTGAQGRKGPRGQPGEKGENGLRGDPGEPGTNINIQGPKGLKGNPGRQGEAGSDGVEGEPGNNGPNGPQGRRGPPGLKGARGFPGEPGNKGDSGIQGIQGSQGMPGPPGPQGPQGLSGRQGDAGAPGASGSFGKPGAPGLKGEPGENGEKGEPGIPGRRGLPGLDGPDGYGPPGLKGEKGHLGFPGDPGPQGEDGDPGIAGNGGPKGISGRRGNSGPPGTNGQRGERGPRGPMGAKGLPGTMPMTPCELVAFTRENCPCFASASKCPVYPTEIVFALDASQDVPPAAFQRMKNTIISIIEKLDISESNCPRGARVAVVAYNSNTKYLIRFSDYKRKSLLLEAIRGISPERSSSRRNIGEAMKFVGRNIFKRIRHGIHVRKVAVFFANGPSPDADAINSAVLEYSALDIIPIVIAFNNVPNVVAAFSADETRRFQVFVWSRPEDQRLELISYCTLCYDKCTPDEECELDQMPATLIDMDIAFVLDSSHNVQADVFEEMKDFVSSMLDTVYISTRPAVSETEARVALVQQAVKDFGPNRNTSPVKTEFDLLRYNSANMMKRHIKQSVRQLNGPSAVGNSVQWVIDNIFLKTPMPRKHKVLFLILASPTSYWDREKLKEVSKKAKCQGFAIFVLGFGKTISELEMTQLPSLPQDQHLLRLISMSKPELVYAKKFSHAFFNLLDRGFNHYPPAVLQAECQGRGDSSFSVVRSFPDTRLTQVDTAAAEVPDETIEVVEVLGTPAPEEIVGKKTEKIIINKGKKANGEVRSLKKDVCTMKQEEGKCADYILKWSYNQGKKACVQFWYGGCGGNGNRFDTQEQCEATCMS

Target ORF information:

RefSeq Version	XM_012965535.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen alpha-6(VI) chain-like (LOC100486063), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965535.1

ORF Insert Sequence:

ATGGGATTTT GGAAAGTATT TCTCGCCTGC GTGCTTGGCA GCTACTCAGT CAATGTCTCA 
GCTTCTCAGA AAACAGTGTG CACAGAGGCC AGTATTGCAG ATATTGTATT CCTTATGGAT 
GGATCCTGGA GCATCGGAAC GGAAAACTTT ATCACAATGA AGAACTTTTT GTACACTTTG 
GTTAATGGTT TTGATGTTGG CCTAGATAAG ATCCAGATTG GCCTAATCCA GTACAGTGAT 
AATGCACGTA CAGAATTCTT CCTAAACAGT TACAGTAACA AAGAAGATGT TTTGAAATAT 
ATTCAGAATC TAAAGTATAA AGGGGGTGGG ACAAAGACTG GCTTAAGTCT GGAGTTCATG 
CTGACGCAGC ACTTTAGTGA AGCTGCAGGC AGCAGAGCAG CAGAAGGTGT GCCTCAGATT 
GCAGTCGTTA TAACTGATGG ACAAGCTCAA GATAGCATCA GGGAGCCTGC CATTGCAGTG 
AAGAACGCTG GAATCATACT GTATGCCATT GGCATTAAAG ATGCAGTTTT ATCTGAGCTT 
AATGAAATAG CCAGTGATCC AGATGACAAA CATGTTTATA GCGTTGCAGA CTTTAATGCA 
CTGCAGAGTA TTTCCCAAAA CATGATCCAG GTGCTCTGCA CAACTGTAGA AGAAGCAGCA 
AGACAGACAG GTCAAATTGC TCAAGTTTGC AGAACTGCCA ATCAAGCTGA CATTGTGCTG 
CTAGTCGAAA GCACAACAAG AATGGGTGAC GCTACTTTTG AAAAAGCAAA GAATTTTCTT 
TATGATTTAG TTTCTAATTT GGATGTTGGA ATTAATAAGA TTCGCATTGG GCTTGTAACC 
TACAATGATG AGACAAATCC AGAATTTTTG CTAAACAGCT ATTCTTCGAA AACTGAAATC 
TTAGAAAGTA TTCAGAATAT GAAATATGTA GAAGGATATA CCTACACAGG GAGAGCCCTT 
GAGTATGTAA ATACAACCTA CTTTACACAG GCAGCAGGCA GTAGATTTGA AGAGAGCGTC 
GCTCAAATTC TTATCATTGT GACAGAAGGA GACTCTTCAG ATACCCTCAC TGAGCCTGCT 
AAAGAACTCA AATCAAGAGG CATATCTGTT TATGTGGTTG GGACAAACAT CAAGTACGAT 
AGACAGCTTC AGGAAGCATC AAGCAAGCCC GACGAAAAGT TCTTTTATCA ACTCGATGAT 
TTTGATGACT CTGAGAATGT CACTGAGCAG CTGTTAAAAA ACCTGTGCTT TAGCATAGAT 
TTGAATATTC AAGTATATTC AAAGCGCTAC GCTGATGTTG TATTTCTAGT CGATTCTTCT 
ACAAGCATGG GAACCATCTT TTTTCAAAAG ATGAAGGATT TCATCATACA CATAATAAAC 
CAGTTAAATG TTGGAATAAA TAAACACCGT ATAGGACTGG CACAGTACAG TGGTCTACCC 
CAAACTGAAT TTCTACTGAA TCATTATGAA ACCAAAGAGG AGATACTAAA GCACATTAAG 
GAAACATTCA CATACAGAGG TGGCCCCTTA AAAACAGGAC ATGCTTTGGA ATTTGTACGC 
TCAACATTTT TCATAGAAGA GGCTGGGAGT AGGATTAACT ACGGCAACCC CCAGTTCTTA 
GTGGTCATAA CATCATCAAA ATCAGAGGAT GCTGTTAGAA GACATGCCGA GGAACTCAAA 
AGTGTTGGGG TCACAACCAT CTCTGTTGGC ATTGGCAATT CTGATCGAAA AGAACTGGAA 
AAAATTGCTA CTGATCCTTT TGTTTTCCAG ACCACAGGTT TACAGCACAT TTCAAATTTA 
CAGCAGGATG TGGCCAATGT TATTATAGCA GAAGACATGC TACAGTTTTC CCCAGTATCA 
GTTGTACCAG CAGTTTGCTC CAGTGCTTCT GTAGCTGACA TTGTGTTTCT AATTGATGAA 
TCTAGCAGCA TTGGCCCCAT CAACTTTCAA CTAACCAGAG TCTTTCTACA CAAGGTTGTT 
AGTGCACTGG ATATTTCCTT GAGTAATGTT AGAGTGGGCT TAGTGCTTTA CAGTGATGAG 
CCAAGACTTG AACTTAAACT GAACACTTTT AATGAAAAAT ATGAAATTTT GGACTTCATA 
ACTAAGCTGC CATACAGAGG AGGCAAAGCA CATACAGGTG CTGCGCTGGA CTTTCTAAGG 
AAAAAAATGT TCACCAAACA AAATGGCGGC CGGCCACATC AAGGAGTTCA GCAGATAGCG 
GTGGTTATGA CTAATGGGCA GTCCATGGAT AATTTTACTA AACCTGCTGC AAAACTGAGA 
CGGTCTGGCG TGGAGGTTTT TGCTGTTGGC TTTCAGAATA TCAATGATAC AGAACTGGAT 
ATCATAGCAT CTCACCCACC GCGAAAACAT GTCACAAATG TGGAATCTTT CCTGCAGCTA 
TCCAACCTTG AGTTCAGAAT ACAGAAACGA CTTTGCAATG AAATTGTTGT GAAGTCATTT 
GCAGTACCTG CAATTGCTCG TGCAGTGAAG GAAGGGTGTG TGGATACAGA AGAAGCAGAT 
ATATACTTTC TAATTGATGG TTCTGGCAGT ATATATCCAG AGGACTTTGA AGATATGAAA 
AAATTCATGA TTGAATTAAT AAGCATGTTT CAAGTTGGTG CCAACAGAGT ACGCTTTGGA 
GTTGTCCAAT ACTCAGATGT TCGTAGAACT GAATTTTTCA TCAGTGAACA CAACACCCAG 
AAAATGTTAA AGGATGCTAT TTCTCAAATA GAACAGTTGG GCGGTGGTAC CCTAACAGGA 
GAAGCTTTAA CGTCAATGAA GCAACTTTTT GTTAATGCAG CTAAAGATCG CCCTCATAAA 
GTACCCCAGT CATTGGTTGT CATTACAGAT GGAGAATCTC AAGACCGGGT CACAGAAGCA 
GCTGCAGAAA TTAGAAATGA TGGCATTACT ATATTTGCAA TTGGTGTTAA AAATGCAGTG 
GAAGAAGAGA TTAGAGATAT AGCAGGGTCA AATGAGAAGA TGTTTTTTGT TAACAACTTT 
GATTCCCTGA AGGTGATCAA GAATGATTTG GCTCGTGAAT TGTGTACACC TGAGGCATGT 
AAGAACATGA AAGCTGATAT CGTGTTTCTG GTTGATAGCT CTGCAAGCAT TAACAGTGAT 
GATTATGAAA CAATGAAAGA ATTCATGGAG TCAATGGTCA AGCAGGCAGA AATCGGCCCT 
GATAGAGTCC AGATTGGTTT AATTCAGTTT AGCTCTGAAA CAAAGGAAGA ATTTCCTCTC 
AACAGGTACA AAAGAAAGGA TGAAATACAA TCAGCAATAA GAGGCATCCA ACAACTGAGC 
CAGGGAACCC TTATGGGGGA AGCCCTTAAA TATACATTAC CTTATTTTAG TGCTTCCAAA 
GGAGGCCGTG TAAATACCAA ACAATATCTG ATCGTCATAA CAGATGGTGA GGCCCAGGAT 
GCTGTGGGAA ACCCAGCCAA AGCAATAAGG GACCATGGAG TTATCATTTA TGCCATAGGA 
GTACAACAAG CCAACAACAC ACAACTTCTG GAGATTGCTG GCAAACAAGA GCAGGTGTAT 
TATGAAGATA GCTTTGATTC CCTAGCTTTT TTGAACAAGA ACATCATGTT TGAGATTTGC 
AATCCTCAAG AATCTTGCAA AAAGACTGAG GTGGCAGACA TCATATTTCT GTTGGATGCT 
TCTGCAAGCA TAACCAGAGG AGAGTTTAGA CTCATGCAGA GATTTGTGGA GGCTGTTGTC 
AATGACTCTT TGGTTGGAAA GGATAATGTT CAATTTGGAG CAGTTGTTTA CGGCACAAAT 
CCAGCAGAAC AGTTTTCACT CAATACGTAT TCAACTAAAC TTGATATTCT TAAGGCTGTA 
TTTAGTTTAC CCCAAGTATC TGGATACACA TACACTGCAA AAGCGCTGGA ATACACTCGG 
ATACGGTTTG GTACATCATA TGGTGGAAGA CCAGGAATTT CACATATTCT AATTCTAGTC 
ACAGATGGAG CCACTACTGA AGCTGATCGC CCTAACCTAC CAATAGTATC AAAGGCTTTG 
AAGGATGATG GCATCATTGT CTTTGCAGTA GGAGTTGGCA AGGCAGTTCC TCAAGAGCTT 
CAGCAGATTG CAGGATACCC AGACAGATGG TTCTTAGTCC AGAACTACAA AGGCCTGGAC 
AATATACATG ATAATATAAC ACAGGTTGTA TGTGATGAAT CAAAACCAGC TTGCTCACAT 
GAACAGCTTG ATCTTGTTTT CCTGATTGAC GGCTCAGCAA GTATCACTTC TAGCAACTTC 
ACAAGTGCCA AGACTTTCAT GAAAGAAATC GTTGACAGCT TTACAATTTC AGAGAACAGA 
GTGCGTATTG GAGTCGCACA GTACAGTGCA AATCCCAAGA AAGAATTCTT CTTAAATGAG 
TACTACTCCA GCTCCGACAT GAAGAAACAG ATTGATAGCA TCTCCCAACT GAAAGCTACC 
ACTTACACTG GCAAAGGCCT ACGATTTGTC AAACAGTTCT TTGATCCAGC AAATGGCGGC 
CGTAAGAATG TTCCGCAGTA CCTGATAGTC ATGACTGATG GCATGTCTAA TGACAGCGTA 
AATGAAGATG CTGCTGCTTT GCGAAGCAGT GGAGTAAAGA TCTTTTCCAT TGGTATTGGT 
CTGCGGAACA GTTTTGAGCT TGTTATGATT GCCGGGTCAC CCAAAAATGT TTACGAAGTG 
GAAACGTTCC AAGCGCTGGA CTCTATAAAG AGGCAGATTG TGGCACAAGT CTGTGAGCCA 
TCTGATCAGC CTATTATAGG TAAAGAATGC AGCTTAGATA TCACCATATC CGTTGACTAC 
TCAAGAAGAG CAATGCTATC TACTGTCCCC CGTGTGCAGC AGAAATTGCA GCAGTTCCTC 
CCTCCGGTGC TTCAGCGCAT TTCCTCGCTT AGCAATATCA GCTGTGCCTC TGGATCTCAA 
ATTAACTTAC GCTTCAGATA CGCTGTTGCA GCACAGGACA GACTGACATT TGATTCCGAC 
TTTGAGAAAT TTAACGAGGA CATCATTAAG AAGTTTATAG CTGAACAGTC TTCTGTTGAT 
ACATATTTAA ATGCAAAGTT TCTTCAGTCC CTCTGGAACA AATACATAGG TTTGCCGTCT 
CAGAAAGCAA AAATTATTCT GGTTTTTACT GATGGACTGG ATGAAAGTTC TGAAGTACTC 
AAGGCGACTA CTGAATCTCT TCGGAAACAA GGGTTGGATG CACTGTTATT CATTGCTCTT 
GAGGGGGTGA AGGATTTTAC AGAGCTGCAA AACTTAGAGT TTGGAAGAGG ATTTTCTTAC 
CAGCAACCTC TGAGCATTGC GCTGCATGAT CTGCCAAGTG TAATGCTAAA AGAAATGGAT 
ACCGTTGCAG AAAGGAAATG CTGTAATGTA GTTTGTAAAT GTTTAGGACA AGATGGACCT 
CTAGGCTCGC CAGGGGCTTT AGGAACAAAG GGTCAGCCTG GTATAAAGGG ATCTCAAGGT 
CATCCTGGAG AGGAAGGCGG TCATGGTGAA AGAGGATTCC GTGGGCTGAA TGGGACAAGA 
GGCGAAAGTG GATGTCCTGG TCGTAGGGGA TTAAAGGGTG CCAGAGGTAT TCGTGGTGAC 
AAGGGTGATG ATGGAGAGTT TGGAATTGAT GGTGTGCCTG GAGAGCAGGG TGAACCTGGA 
GGGCGTGGAG CATCAGGAGA ACGTGGAGAC ACTGGAGCTC AGGGCAGAAA AGGTCCTCGT 
GGGCAACCTG GAGAAAAAGG GGAAAATGGA CTGAGGGGAG ACCCAGGTGA GCCTGGAACT 
AACATTAACA TTCAGGGACC TAAAGGACTG AAAGGAAACC CAGGACGTCA GGGAGAGGCG 
GGAAGTGACG GTGTGGAAGG AGAGCCAGGA AATAATGGAC CTAATGGTCC ACAAGGCCGA 
AGAGGGCCTC CTGGTTTAAA GGGAGCACGA GGATTTCCTG GAGAGCCAGG GAACAAAGGT 
GATAGTGGAA TCCAAGGTAT ACAGGGTTCC CAAGGCATGC CTGGACCACC AGGTCCACAG 
GGACCACAAG GGTTATCTGG ACGTCAGGGA GATGCAGGTG CCCCTGGTGC ATCAGGTTCC 
TTTGGCAAAC CCGGAGCTCC AGGACTAAAG GGGGAACCTG GAGAAAACGG AGAGAAGGGT 
GAGCCAGGCA TTCCGGGTCG CAGAGGTTTG CCAGGGCTGG ATGGTCCTGA TGGATATGGT 
CCCCCAGGCT TAAAGGGAGA GAAGGGTCAT TTAGGGTTTC CTGGGGATCC TGGGCCTCAG 
GGAGAAGATG GTGATCCTGG AATAGCTGGA AATGGTGGCC CAAAAGGAAT TAGTGGAAGA 
AGGGGTAATT CTGGCCCTCC AGGAACTAAT GGACAAAGGG GTGAGAGGGG ACCACGTGGA 
CCTATGGGCG CCAAAGGATT GCCAGGAACT ATGCCTATGA CGCCTTGTGA ACTTGTGGCA 
TTTACTCGAG AGAATTGTCC TTGCTTCGCA AGTGCTTCAA AATGTCCAGT TTACCCAACT 
GAAATAGTGT TTGCTCTGGA TGCATCTCAG GACGTTCCCC CGGCTGCTTT CCAAAGGATG 
AAGAACACCA TTATTTCCAT AATAGAAAAG CTGGACATTA GTGAGAGCAA TTGCCCTAGA 
GGAGCCCGTG TTGCTGTTGT TGCGTATAAC AGTAATACCA AATACCTCAT TCGTTTCTCT 
GACTACAAAC GGAAAAGCCT ACTGCTGGAA GCAATTCGTG GCATCTCTCC AGAAAGATCA 
TCTTCAAGGA GAAATATTGG AGAAGCCATG AAGTTTGTAG GGAGGAACAT ATTCAAACGC 
ATTCGTCATG GAATCCATGT TAGAAAAGTG GCTGTGTTCT TTGCTAATGG CCCATCCCCA 
GATGCAGATG CAATAAACTC GGCAGTGCTG GAATACAGTG CTCTTGACAT TATTCCTATT 
GTGATTGCAT TTAATAATGT TCCCAATGTC GTAGCAGCTT TTTCGGCCGA CGAAACTAGA 
AGATTCCAAG TGTTTGTATG GAGTAGGCCT GAGGATCAGA GACTAGAACT TATTTCTTAT 
TGTACTCTTT GTTATGATAA GTGTACACCT GATGAAGAAT GTGAGCTGGA TCAAATGCCT 
GCAACCCTAA TAGATATGGA CATAGCTTTT GTATTAGACA GTTCACACAA TGTGCAAGCT 
GATGTCTTTG AGGAAATGAA AGACTTTGTG AGCAGTATGC TGGACACCGT CTACATATCA 
ACGCGGCCCG CAGTTTCTGA AACAGAAGCC AGAGTTGCCT TAGTACAACA GGCAGTTAAG 
GACTTTGGTC CAAATAGGAA TACTAGCCCA GTTAAAACTG AGTTCGACCT TCTTCGCTAC 
AACAGCGCAA ATATGATGAA GAGGCACATC AAACAATCTG TCAGGCAACT GAATGGTCCT 
TCTGCAGTTG GCAATTCTGT TCAGTGGGTC ATTGATAATA TCTTCTTAAA AACACCAATG 
CCAAGAAAGC ATAAAGTACT TTTCCTCATT CTTGCCAGCC CAACAAGCTA TTGGGACAGA 
GAAAAATTGA AAGAAGTCTC CAAAAAAGCT AAGTGTCAAG GATTTGCAAT ATTTGTACTG 
GGTTTTGGAA AAACGATAAG TGAGCTGGAA ATGACACAGC TGCCGAGCTT GCCACAAGAC 
CAACATTTGC TTCGCTTGAT AAGCATGTCA AAACCAGAAC TTGTCTATGC AAAGAAGTTC 
AGTCATGCCT TCTTCAATCT ACTCGACCGT GGCTTTAATC ACTACCCACC CGCAGTATTG 
CAAGCAGAGT GCCAAGGGCG TGGGGACTCA TCTTTTTCAG TTGTAAGAAG CTTCCCTGAC 
ACACGGTTGA CACAAGTTGA CACTGCTGCA GCAGAAGTTC CAGATGAGAC TATAGAAGTA 
GTGGAGGTCC TTGGTACACC TGCACCTGAA GAAATTGTGG GGAAAAAAAC AGAGAAAATA 
ATCATAAACA AAGGTAAAAA AGCTAATGGG GAAGTGCGGA GCCTTAAGAA AGATGTCTGT 
ACAATGAAAC AAGAGGAAGG AAAATGTGCA GATTACATCC TCAAGTGGTC CTACAACCAA 
GGCAAAAAAG CTTGTGTGCA GTTCTGGTAC GGTGGCTGTG GAGGAAATGG CAATCGTTTT 
GACACACAGG AACAGTGTGA AGCCACCTGC ATGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col6a5 ( XM_012965536.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820990.1 Xenopus tropicalis collagen alpha-6(VI) chain-like (LOC100486063), transcript variant X3, mRNA.

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CloneID	OXa33519
Clone ID Related Accession (Same CDS sequence)	XM_012965536.1
Accession Version	XM_012965536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-6(VI) chain-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTTT GGAAAGTATT TCTCGCCTGC GTGCTTGGCA GCTACTCAGT CAATGTCTCA 
GCTTCTCAGA AAACAGTGTG CACAGAGGCC AGTATTGCAG ATATTGTATT CCTTATGGAT 
GGATCCTGGA GCATCGGAAC GGAAAACTTT ATCACAATGA AGAACTTTTT GTACACTTTG 
GTTAATGGTT TTGATGTTGG CCTAGATAAG ATCCAGATTG GCCTAATCCA GTACAGTGAT 
AATGCACGTA CAGAATTCTT CCTAAACAGT TACAGTAACA AAGAAGATGT TTTGAAATAT 
ATTCAGAATC TAAAGTATAA AGGGGGTGGG ACAAAGACTG GCTTAAGTCT GGAGTTCATG 
CTGACGCAGC ACTTTAGTGA AGCTGCAGGC AGCAGAGCAG CAGAAGGTGT GCCTCAGATT 
GCAGTCGTTA TAACTGATGG ACAAGCTCAA GATAGCATCA GGGAGCCTGC CATTGCAGTG 
AAGAACGCTG GAATCATACT GTATGCCATT GGCATTAAAG ATGCAGTTTT ATCTGAGCTT 
AATGAAATAG CCAGTGATCC AGATGACAAA CATGTTTATA GCGTTGCAGA CTTTAATGCA 
CTGCAGAGTA TTTCCCAAAA CATGATCCAG GTGCTCTGCA CAACTGTAGA AGAAGCAGCA 
AGACAGACAG GTCAAATTGC TCAAGTTTGC AGAACTGCCA ATCAAGCTGA CATTGTGCTG 
CTAGTCGAAA GCACAACAAG AATGGGTGAC GCTACTTTTG AAAAAGCAAA GAATTTTCTT 
TATGATTTAG TTTCTAATTT GGATGTTGGA ATTAATAAGA TTCGCATTGG GCTTGTAACC 
TACAATGATG AGACAAATCC AGAATTTTTG CTAAACAGCT ATTCTTCGAA AACTGAAATC 
TTAGAAAGTA TTCAGAATAT GAAATATGTA GAAGGATATA CCTACACAGG GAGAGCCCTT 
GAGTATGTAA ATACAACCTA CTTTACACAG GCAGCAGGCA GTAGATTTGA AGAGAGCGTC 
GCTCAAATTC TTATCATTGT GACAGAAGGA GACTCTTCAG ATACCCTCAC TGAGCCTGCT 
AAAGAACTCA AATCAAGAGG CATATCTGTT TATGTGGTTG GGACAAACAT CAAGTACGAT 
AGACAGCTTC AGGAAGCATC AAGCAAGCCC GACGAAAAGT TCTTTTATCA ACTCGATGAT 
TTTGATGACT CTGAGAATGT CACTGAGCAG CTGTTAAAAA ACCTGTGCTT TAGCATAGAT 
TTGAATATTC AAGTATATTC AAAGCGCTAC GCTGATGTTG TATTTCTAGT CGATTCTTCT 
ACAAGCATGG GAACCATCTT TTTTCAAAAG ATGAAGGATT TCATCATACA CATAATAAAC 
CAGTTAAATG TTGGAATAAA TAAACACCGT ATAGGACTGG CACAGTACAG TGGTCTACCC 
CAAACTGAAT TTCTACTGAA TCATTATGAA ACCAAAGAGG AGATACTAAA GCACATTAAG 
GAAACATTCA CATACAGAGG TGGCCCCTTA AAAACAGGAC ATGCTTTGGA ATTTGTACGC 
TCAACATTTT TCATAGAAGA GGCTGGGAGT AGGATTAACT ACGGCAACCC CCAGTTCTTA 
GTGGTCATAA CATCATCAAA ATCAGAGGAT GCTGTTAGAA GACATGCCGA GGAACTCAAA 
AGTGTTGGGG TCACAACCAT CTCTGTTGGC ATTGGCAATT CTGATCGAAA AGAACTGGAA 
AAAATTGCTA CTGATCCTTT TGTTTTCCAG ACCACAGGTT TACAGCACAT TTCAAATTTA 
CAGCAGGATG TGGCCAATGT TATTATAGCA GAAGACATGC TACAGTTTTC CCCAGTATCA 
GTTGTACCAG CAGTTTGCTC CAGTGCTTCT GTAGCTGACA TTGTGTTTCT AATTGATGAA 
TCTAGCAGCA TTGGCCCCAT CAACTTTCAA CTAACCAGAG TCTTTCTACA CAAGGTTGTT 
AGTGCACTGG ATATTTCCTT GAGTAATGTT AGAGTGGGCT TAGTGCTTTA CAGTGATGAG 
CCAAGACTTG AACTTAAACT GAACACTTTT AATGAAAAAT ATGAAATTTT GGACTTCATA 
ACTAAGCTGC CATACAGAGG AGGCAAAGCA CATACAGGTG CTGCGCTGGA CTTTCTAAGG 
AAAAAAATGT TCACCAAACA AAATGGCGGC CGGCCACATC AAGGAGTTCA GCAGATAGCG 
GTGGTTATGA CTAATGGGCA GTCCATGGAT AATTTTACTA AACCTGCTGC AAAACTGAGA 
CGGTCTGGCG TGGAGGTTTT TGCTGTTGGC TTTCAGAATA TCAATGATAC AGAACTGGAT 
ATCATAGCAT CTCACCCACC GCGAAAACAT GTCACAAATG TGGAATCTTT CCTGCAGCTA 
TCCAACCTTG AGTTCAGAAT ACAGAAACGA CTTTGCAATG AAATTGTTGT GAAGTCATTT 
GCAGTACCTG CAATTGCTCG TGCAGTGAAG GAAGGGTGTG TGGATACAGA AGAAGCAGAT 
ATATACTTTC TAATTGATGG TTCTGGCAGT ATATATCCAG AGGACTTTGA AGATATGAAA 
AAATTCATGA TTGAATTAAT AAGCATGTTT CAAGTTGGTG CCAACAGAGT ACGCTTTGGA 
GTTGTCCAAT ACTCAGATGT TCGTAGAACT GAATTTTTCA TCAGTGAACA CAACACCCAG 
AAAATGTTAA AGGATGCTAT TTCTCAAATA GAACAGTTGG GCGGTGGTAC CCTAACAGGA 
GAAGCTTTAA CGTCAATGAA GCAACTTTTT GTTAATGCAG CTAAAGATCG CCCTCATAAA 
GTACCCCAGT CATTGGTTGT CATTACAGAT GGAGAATCTC AAGACCGGGT CACAGAAGCA 
GCTGCAGAAA TTAGAAATGA TGGCATTACT ATATTTGCAA TTGGTGTTAA AAATGCAGTG 
GAAGAAGAGA TTAGAGATAT AGCAGGGTCA AATGAGAAGA TGTTTTTTGT TAACAACTTT 
GATTCCCTGA AGGTGATCAA GAATGATTTG GCTCGTGAAT TGTGTACACC TGAGGCATGT 
AAGAACATGA AAGCTGATAT CGTGTTTCTG GTTGATAGCT CTGCAAGCAT TAACAGTGAT 
GATTATGAAA CAATGAAAGA ATTCATGGAG TCAATGGTCA AGCAGGCAGA AATCGGCCCT 
GATAGAGTCC AGATTGGTTT AATTCAGTTT AGCTCTGAAA CAAAGGAAGA ATTTCCTCTC 
AACAGGTACA AAAGAAAGGA TGAAATACAA TCAGCAATAA GAGGCATCCA ACAACTGAGC 
CAGGGAACCC TTATGGGGGA AGCCCTTAAA TATACATTAC CTTATTTTAG TGCTTCCAAA 
GGAGGCCGTG TAAATACCAA ACAATATCTG ATCGTCATAA CAGATGGTGA GGCCCAGGAT 
GCTGTGGGAA ACCCAGCCAA AGCAATAAGG GACCATGGAG TTATCATTTA TGCCATAGGA 
GTACAACAAG CCAACAACAC ACAACTTCTG GAGATTGCTG GCAAACAAGA GCAGGTGTAT 
TATGAAGATA GCTTTGATTC CCTAGCTTTT TTGAACAAGA ACATCATGTT TGAGATTTGC 
AATCCTCAAG AATCTTGCAA AAAGACTGAG GTGGCAGACA TCATATTTCT GTTGGATGCT 
TCTGCAAGCA TAACCAGAGG AGAGTTTAGA CTCATGCAGA GATTTGTGGA GGCTGTTGTC 
AATGACTCTT TGGTTGGAAA GGATAATGTT CAATTTGGAG CAGTTGTTTA CGGCACAAAT 
CCAGCAGAAC AGTTTTCACT CAATACGTAT TCAACTAAAC TTGATATTCT TAAGGCTGTA 
TTTAGTTTAC CCCAAGTATC TGGATACACA TACACTGCAA AAGCGCTGGA ATACACTCGG 
ATACGGTTTG GTACATCATA TGGTGGAAGA CCAGGAATTT CACATATTCT AATTCTAGTC 
ACAGATGGAG CCACTACTGA AGCTGATCGC CCTAACCTAC CAATAGTATC AAAGGCTTTG 
AAGGATGATG GCATCATTGT CTTTGCAGTA GGAGTTGGCA AGGCAGTTCC TCAAGAGCTT 
CAGCAGATTG CAGGATACCC AGACAGATGG TTCTTAGTCC AGAACTACAA AGGCCTGGAC 
AATATACATG ATAATATAAC ACAGGTTGTA TGTGATGAAT CAAAACCAGC TTGCTCACAT 
GAACAGCTTG ATCTTGTTTT CCTGATTGAC GGCTCAGCAA GTATCACTTC TAGCAACTTC 
ACAAGTGCCA AGACTTTCAT GAAAGAAATC GTTGACAGCT TTACAATTTC AGAGAACAGA 
GTGCGTATTG GAGTCGCACA GTACAGTGCA AATCCCAAGA AAGAATTCTT CTTAAATGAG 
TACTACTCCA GCTCCGACAT GAAGAAACAG ATTGATAGCA TCTCCCAACT GAAAGCTACC 
ACTTACACTG GCAAAGGCCT ACGATTTGTC AAACAGTTCT TTGATCCAGC AAATGGCGGC 
CGTAAGAATG TTCCGCAGTA CCTGATAGTC ATGACTGATG GCATGTCTAA TGACAGCGTA 
AATGAAGATG CTGCTGCTTT GCGAAGCAGT GGAGTAAAGA TCTTTTCCAT TGGTATTGGT 
CTGCGGAACA GTTTTGAGCT TGTTATGATT GCCGGGTCAC CCAAAAATGT TTACGAAGTG 
GAAACGTTCC AAGCGCTGGA CTCTATAAAG AGGCAGATTG TGGCACAAGT CTGTGAGCCA 
TCTGATCAGC CTATTATAGG TAAAGAATGC AGCTTAGATA TCACCATATC CGTTGACTAC 
TCAAGAAGAG CAATGCTATC TACTGTCCCC CGTGTGCAGC AGAAATTGCA GCAGTTCCTC 
CCTCCGGTGC TTCAGCGCAT TTCCTCGCTT AGCAATATCA GCTGTGCCTC TGGATCTCAA 
ATTAACTTAC GCTTCAGATA CGCTGTTGCA GCACAGGACA GACTGACATT TGATTCCGAC 
TTTGAGAAAT TTAACGAGGA CATCATTAAG AAGTTTATAG CTGAACAGTC TTCTGTTGAT 
ACATATTTAA ATGCAAAGTT TCTTCAGTCC CTCTGGAACA AATACATAGG TTTGCCGTCT 
CAGAAAGCAA AAATTATTCT GGTTTTTACT GATGGACTGG ATGAAAGTTC TGAAGTACTC 
AAGGCGACTA CTGAATCTCT TCGGAAACAA GGGTTGGATG CACTGTTATT CATTGCTCTT 
GAGGGGGTGA AGGATTTTAC AGAGCTGCAA AACTTAGAGT TTGGAAGAGG ATTTTCTTAC 
CAGCAACCTC TGAGCATTGC GCTGCATGAT CTGCCAAGTG TAATGCTAAA AGAAATGGAT 
ACCGTTGCAG AAAGGAAATG CTGTAATGTA GTTTGTAAAT GTTTAGGACA AGATGGACCT 
CTAGGCTCGC CAGGGGCTTT AGGAACAAAG GGTCAGCCTG GTATAAAGGG ATCTCAAGGT 
CATCCTGGAG AGGAAGGCGG TCATGGTGAA AGAGGATTCC GTGGGCTGAA TGGGACAAGA 
GGCGAAAGTG GATGTCCTGG TCGTAGGGGA TTAAAGGGTG CCAGAGGTAT TCGTGGTGAC 
AAGGGTGATG ATGGAGAGTT TGGAATTGAT GGTGTGCCTG GAGAGCAGGG TGAACCTGGA 
GGGCGTGGAG CATCAGGAGA ACGTGGAGAC ACTGGAGCTC AGGGCAGAAA AGGTCCTCGT 
GGGCAACCTG GAGAAAAAGG GGAAAATGGA CTGAGGGGAG ACCCAGGTGA GCCTGGAACT 
AACATTAACA TTCAGGGACC TAAAGGACTG AAAGGAAACC CAGGACGTCA GGGAGAGGCG 
GGAAGTGACG GTGTGGAAGG AGAGCCAGGA AATAATGGAC CTAATGGTCC ACAAGGCCGA 
AGAGGGCCTC CTGGTTTAAA GGGAGCACGA GGATTTCCTG GAGAGCCAGG GAACAAAGGT 
GATAGTGGAA TCCAAGGTAT ACAGGGTTCC CAAGGCATGC CTGGACCACC AGGTCCACAG 
GGACCACAAG GGTTATCTGG ACGTCAGGGA GATGCAGGTG CCCCTGGTGC ATCAGGTTCC 
TTTGGCAAAC CCGGAGCTCC AGGACTAAAG GGGGAACCTG GAGAAAACGG AGAGAAGGGT 
GAGCCAGGCA TTCCGGGTCG CAGAGGTTTG CCAGGGCTGG ATGGTCCTGA TGGATATGGT 
CCCCCAGGCT TAAAGGGAGA GAAGGGTCAT TTAGGGTTTC CTGGGGATCC TGGGCCTCAG 
GGAGAAGATG GTGATCCTGG AATAGCTGGA AATGGTGGCC CAAAAGGAAT TAGTGGAAGA 
AGGGGTAATT CTGGCCCTCC AGGAACTAAT GGACAAAGGG GTGAGAGGGG ACCACGTGGA 
CCTATGGGCG CCAAAGGATT GCCAGGAACT ATGCCTATGA CGCCTTGTGA ACTTGTGGCA 
TTTACTCGAG AGAATTGTCC TTGCTTCGCA AGTGCTTCAA AATGTCCAGT TTACCCAACT 
GAAATAGTGT TTGCTCTGGA TGCATCTCAG GACGTTCCCC CGGCTGCTTT CCAAAGGATG 
AAGAACACCA TTATTTCCAT AATAGAAAAG CTGGACATTA GTGAGAGCAA TTGCCCTAGA 
GGAGCCCGTG TTGCTGTTGT TGCGTATAAC AGTAATACCA AATACCTCAT TCGTTTCTCT 
GACTACAAAC GGAAAAGCCT ACTGCTGGAA GCAATTCGTG GCATCTCTCC AGAAAGATCA 
TCTTCAAGGA GAAATATTGG AGAAGCCATG AAGTTTGTAG GGAGGAACAT ATTCAAACGC 
ATTCGTCATG GAATCCATGT TAGAAAAGTG GCTGTGTTCT TTGCTAATGG CCCATCCCCA 
GATGCAGATG CAATAAACTC GGCAGTGCTG GAATACAGTG CTCTTGACAT TATTCCTATT 
GTGATTGCAT TTAATAATGT TCCCAATGTC GTAGCAGCTT TTTCGGCCGA CGAAACTAGA 
AGATTCCAAG TGTTTGTATG GAGTAGGCCT GAGGATCAGA GACTAGAACT TATTTCTTAT 
TGTACTCTTT GTTATGATAA GTGTACACCT GATGAAGAAT GTGAGCTGGA TCAAATGCCT 
GCAACCCTAA TAGATATGGA CATAGCTTTT GTATTAGACA GTTCACACAA TGTGCAAGCT 
GATGTCTTTG AGGAAATGAA AGACTTTGTG AGCAGTATGC TGGACACCGT CTACATATCA 
ACGCGGCCCG CAGTTTCTGA AACAGAAGCC AGAGTTGCCT TAGTACAACA GGCAGTTAAG 
GACTTTGGTC CAAATAGGAA TACTAGCCCA GTTAAAACTG AGTTCGACCT TCTTCGCTAC 
AACAGCGCAA ATATGATGAA GAGGCACATC AAACAATCTG TCAGGCAACT GAATGGTCCT 
TCTGCAGTTG GCAATTCTGT TCAGTGGGTC ATTGATAATA TCTTCTTAAA AACACCAATG 
CCAAGAAAGC ATAAAGTACT TTTCCTCATT CTTGCCAGCC CAACAAGCTA TTGGGACAGA 
GAAAAATTGA AAGAAGTCTC CAAAAAAGCT AAGTGTCAAG GATTTGCAAT ATTTGTACTG 
GGTTTTGGAA AAACGATAAG TGAGCTGGAA ATGACACAGC TGCCGAGCTT GCCACAAGAC 
CAACATTTGC TTCGCTTGAT AAGCATGTCA AAACCAGAAC TTGTCTATGC AAAGAAGTTC 
AGTCATGCCT TCTTCAATCT ACTCGACCGT GGCTTTAATC ACTACCCACC CGCAGTATTG 
CAAGCAGAGT GCCAAGGGCG TGGGGACTCA TCTTTTTCAG TTGTAAGAAG CTTCCCTGAC 
ACACGGTTGA CACAAGTTGA CACTGCTGCA GCAGAAGTTC CAGATGAGAC TATAGAAGTA 
GTGGAGGTCC TTGGTACACC TGCACCTGAA GAAATTGTGG GGAAAAAAAC AGAGAAAATA 
ATCATAAACA AAGATGTCTG TACAATGAAA CAAGAGGAAG GAAAATGTGC AGATTACATC 
CTCAAGTGGT CCTACAACCA AGGCAAAAAA GCTTGTGTGC AGTTCTGGTA CGGTGGCTGT 
GGAGGAAATG GCAATCGTTT TGACACACAG GAACAGTGTG AAGCCACCTG CATGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820990.1
CDS	129..8228
Translation	MGFWKVFLACVLGSYSVNVSASQKTVCTEASIADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREPAIAVKNAGIILYAIGIKDAVLSELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEPAKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRRHAEELKSVGVTTISVGIGNSDRKELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKPAAKLRRSGVEVFAVGFQNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELCTPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNPAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNPQESCKKTEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVPQELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVNEDAAALRSSGVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQVCEPSDQPIIGKECSLDITISVDYSRRAMLSTVPRVQQKLQQFLPPVLQRISSLSNISCASGSQINLRFRYAVAAQDRLTFDSDFEKFNEDIIKKFIAEQSSVDTYLNAKFLQSLWNKYIGLPSQKAKIILVFTDGLDESSEVLKATTESLRKQGLDALLFIALEGVKDFTELQNLEFGRGFSYQQPLSIALHDLPSVMLKEMDTVAERKCCNVVCKCLGQDGPLGSPGALGTKGQPGIKGSQGHPGEEGGHGERGFRGLNGTRGESGCPGRRGLKGARGIRGDKGDDGEFGIDGVPGEQGEPGGRGASGERGDTGAQGRKGPRGQPGEKGENGLRGDPGEPGTNINIQGPKGLKGNPGRQGEAGSDGVEGEPGNNGPNGPQGRRGPPGLKGARGFPGEPGNKGDSGIQGIQGSQGMPGPPGPQGPQGLSGRQGDAGAPGASGSFGKPGAPGLKGEPGENGEKGEPGIPGRRGLPGLDGPDGYGPPGLKGEKGHLGFPGDPGPQGEDGDPGIAGNGGPKGISGRRGNSGPPGTNGQRGERGPRGPMGAKGLPGTMPMTPCELVAFTRENCPCFASASKCPVYPTEIVFALDASQDVPPAAFQRMKNTIISIIEKLDISESNCPRGARVAVVAYNSNTKYLIRFSDYKRKSLLLEAIRGISPERSSSRRNIGEAMKFVGRNIFKRIRHGIHVRKVAVFFANGPSPDADAINSAVLEYSALDIIPIVIAFNNVPNVVAAFSADETRRFQVFVWSRPEDQRLELISYCTLCYDKCTPDEECELDQMPATLIDMDIAFVLDSSHNVQADVFEEMKDFVSSMLDTVYISTRPAVSETEARVALVQQAVKDFGPNRNTSPVKTEFDLLRYNSANMMKRHIKQSVRQLNGPSAVGNSVQWVIDNIFLKTPMPRKHKVLFLILASPTSYWDREKLKEVSKKAKCQGFAIFVLGFGKTISELEMTQLPSLPQDQHLLRLISMSKPELVYAKKFSHAFFNLLDRGFNHYPPAVLQAECQGRGDSSFSVVRSFPDTRLTQVDTAAAEVPDETIEVVEVLGTPAPEEIVGKKTEKIIINKDVCTMKQEEGKCADYILKWSYNQGKKACVQFWYGGCGGNGNRFDTQEQCEATCMS

Target ORF information:

RefSeq Version	XM_012965536.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen alpha-6(VI) chain-like (LOC100486063), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965536.1

ORF Insert Sequence:

ATGGGATTTT GGAAAGTATT TCTCGCCTGC GTGCTTGGCA GCTACTCAGT CAATGTCTCA 
GCTTCTCAGA AAACAGTGTG CACAGAGGCC AGTATTGCAG ATATTGTATT CCTTATGGAT 
GGATCCTGGA GCATCGGAAC GGAAAACTTT ATCACAATGA AGAACTTTTT GTACACTTTG 
GTTAATGGTT TTGATGTTGG CCTAGATAAG ATCCAGATTG GCCTAATCCA GTACAGTGAT 
AATGCACGTA CAGAATTCTT CCTAAACAGT TACAGTAACA AAGAAGATGT TTTGAAATAT 
ATTCAGAATC TAAAGTATAA AGGGGGTGGG ACAAAGACTG GCTTAAGTCT GGAGTTCATG 
CTGACGCAGC ACTTTAGTGA AGCTGCAGGC AGCAGAGCAG CAGAAGGTGT GCCTCAGATT 
GCAGTCGTTA TAACTGATGG ACAAGCTCAA GATAGCATCA GGGAGCCTGC CATTGCAGTG 
AAGAACGCTG GAATCATACT GTATGCCATT GGCATTAAAG ATGCAGTTTT ATCTGAGCTT 
AATGAAATAG CCAGTGATCC AGATGACAAA CATGTTTATA GCGTTGCAGA CTTTAATGCA 
CTGCAGAGTA TTTCCCAAAA CATGATCCAG GTGCTCTGCA CAACTGTAGA AGAAGCAGCA 
AGACAGACAG GTCAAATTGC TCAAGTTTGC AGAACTGCCA ATCAAGCTGA CATTGTGCTG 
CTAGTCGAAA GCACAACAAG AATGGGTGAC GCTACTTTTG AAAAAGCAAA GAATTTTCTT 
TATGATTTAG TTTCTAATTT GGATGTTGGA ATTAATAAGA TTCGCATTGG GCTTGTAACC 
TACAATGATG AGACAAATCC AGAATTTTTG CTAAACAGCT ATTCTTCGAA AACTGAAATC 
TTAGAAAGTA TTCAGAATAT GAAATATGTA GAAGGATATA CCTACACAGG GAGAGCCCTT 
GAGTATGTAA ATACAACCTA CTTTACACAG GCAGCAGGCA GTAGATTTGA AGAGAGCGTC 
GCTCAAATTC TTATCATTGT GACAGAAGGA GACTCTTCAG ATACCCTCAC TGAGCCTGCT 
AAAGAACTCA AATCAAGAGG CATATCTGTT TATGTGGTTG GGACAAACAT CAAGTACGAT 
AGACAGCTTC AGGAAGCATC AAGCAAGCCC GACGAAAAGT TCTTTTATCA ACTCGATGAT 
TTTGATGACT CTGAGAATGT CACTGAGCAG CTGTTAAAAA ACCTGTGCTT TAGCATAGAT 
TTGAATATTC AAGTATATTC AAAGCGCTAC GCTGATGTTG TATTTCTAGT CGATTCTTCT 
ACAAGCATGG GAACCATCTT TTTTCAAAAG ATGAAGGATT TCATCATACA CATAATAAAC 
CAGTTAAATG TTGGAATAAA TAAACACCGT ATAGGACTGG CACAGTACAG TGGTCTACCC 
CAAACTGAAT TTCTACTGAA TCATTATGAA ACCAAAGAGG AGATACTAAA GCACATTAAG 
GAAACATTCA CATACAGAGG TGGCCCCTTA AAAACAGGAC ATGCTTTGGA ATTTGTACGC 
TCAACATTTT TCATAGAAGA GGCTGGGAGT AGGATTAACT ACGGCAACCC CCAGTTCTTA 
GTGGTCATAA CATCATCAAA ATCAGAGGAT GCTGTTAGAA GACATGCCGA GGAACTCAAA 
AGTGTTGGGG TCACAACCAT CTCTGTTGGC ATTGGCAATT CTGATCGAAA AGAACTGGAA 
AAAATTGCTA CTGATCCTTT TGTTTTCCAG ACCACAGGTT TACAGCACAT TTCAAATTTA 
CAGCAGGATG TGGCCAATGT TATTATAGCA GAAGACATGC TACAGTTTTC CCCAGTATCA 
GTTGTACCAG CAGTTTGCTC CAGTGCTTCT GTAGCTGACA TTGTGTTTCT AATTGATGAA 
TCTAGCAGCA TTGGCCCCAT CAACTTTCAA CTAACCAGAG TCTTTCTACA CAAGGTTGTT 
AGTGCACTGG ATATTTCCTT GAGTAATGTT AGAGTGGGCT TAGTGCTTTA CAGTGATGAG 
CCAAGACTTG AACTTAAACT GAACACTTTT AATGAAAAAT ATGAAATTTT GGACTTCATA 
ACTAAGCTGC CATACAGAGG AGGCAAAGCA CATACAGGTG CTGCGCTGGA CTTTCTAAGG 
AAAAAAATGT TCACCAAACA AAATGGCGGC CGGCCACATC AAGGAGTTCA GCAGATAGCG 
GTGGTTATGA CTAATGGGCA GTCCATGGAT AATTTTACTA AACCTGCTGC AAAACTGAGA 
CGGTCTGGCG TGGAGGTTTT TGCTGTTGGC TTTCAGAATA TCAATGATAC AGAACTGGAT 
ATCATAGCAT CTCACCCACC GCGAAAACAT GTCACAAATG TGGAATCTTT CCTGCAGCTA 
TCCAACCTTG AGTTCAGAAT ACAGAAACGA CTTTGCAATG AAATTGTTGT GAAGTCATTT 
GCAGTACCTG CAATTGCTCG TGCAGTGAAG GAAGGGTGTG TGGATACAGA AGAAGCAGAT 
ATATACTTTC TAATTGATGG TTCTGGCAGT ATATATCCAG AGGACTTTGA AGATATGAAA 
AAATTCATGA TTGAATTAAT AAGCATGTTT CAAGTTGGTG CCAACAGAGT ACGCTTTGGA 
GTTGTCCAAT ACTCAGATGT TCGTAGAACT GAATTTTTCA TCAGTGAACA CAACACCCAG 
AAAATGTTAA AGGATGCTAT TTCTCAAATA GAACAGTTGG GCGGTGGTAC CCTAACAGGA 
GAAGCTTTAA CGTCAATGAA GCAACTTTTT GTTAATGCAG CTAAAGATCG CCCTCATAAA 
GTACCCCAGT CATTGGTTGT CATTACAGAT GGAGAATCTC AAGACCGGGT CACAGAAGCA 
GCTGCAGAAA TTAGAAATGA TGGCATTACT ATATTTGCAA TTGGTGTTAA AAATGCAGTG 
GAAGAAGAGA TTAGAGATAT AGCAGGGTCA AATGAGAAGA TGTTTTTTGT TAACAACTTT 
GATTCCCTGA AGGTGATCAA GAATGATTTG GCTCGTGAAT TGTGTACACC TGAGGCATGT 
AAGAACATGA AAGCTGATAT CGTGTTTCTG GTTGATAGCT CTGCAAGCAT TAACAGTGAT 
GATTATGAAA CAATGAAAGA ATTCATGGAG TCAATGGTCA AGCAGGCAGA AATCGGCCCT 
GATAGAGTCC AGATTGGTTT AATTCAGTTT AGCTCTGAAA CAAAGGAAGA ATTTCCTCTC 
AACAGGTACA AAAGAAAGGA TGAAATACAA TCAGCAATAA GAGGCATCCA ACAACTGAGC 
CAGGGAACCC TTATGGGGGA AGCCCTTAAA TATACATTAC CTTATTTTAG TGCTTCCAAA 
GGAGGCCGTG TAAATACCAA ACAATATCTG ATCGTCATAA CAGATGGTGA GGCCCAGGAT 
GCTGTGGGAA ACCCAGCCAA AGCAATAAGG GACCATGGAG TTATCATTTA TGCCATAGGA 
GTACAACAAG CCAACAACAC ACAACTTCTG GAGATTGCTG GCAAACAAGA GCAGGTGTAT 
TATGAAGATA GCTTTGATTC CCTAGCTTTT TTGAACAAGA ACATCATGTT TGAGATTTGC 
AATCCTCAAG AATCTTGCAA AAAGACTGAG GTGGCAGACA TCATATTTCT GTTGGATGCT 
TCTGCAAGCA TAACCAGAGG AGAGTTTAGA CTCATGCAGA GATTTGTGGA GGCTGTTGTC 
AATGACTCTT TGGTTGGAAA GGATAATGTT CAATTTGGAG CAGTTGTTTA CGGCACAAAT 
CCAGCAGAAC AGTTTTCACT CAATACGTAT TCAACTAAAC TTGATATTCT TAAGGCTGTA 
TTTAGTTTAC CCCAAGTATC TGGATACACA TACACTGCAA AAGCGCTGGA ATACACTCGG 
ATACGGTTTG GTACATCATA TGGTGGAAGA CCAGGAATTT CACATATTCT AATTCTAGTC 
ACAGATGGAG CCACTACTGA AGCTGATCGC CCTAACCTAC CAATAGTATC AAAGGCTTTG 
AAGGATGATG GCATCATTGT CTTTGCAGTA GGAGTTGGCA AGGCAGTTCC TCAAGAGCTT 
CAGCAGATTG CAGGATACCC AGACAGATGG TTCTTAGTCC AGAACTACAA AGGCCTGGAC 
AATATACATG ATAATATAAC ACAGGTTGTA TGTGATGAAT CAAAACCAGC TTGCTCACAT 
GAACAGCTTG ATCTTGTTTT CCTGATTGAC GGCTCAGCAA GTATCACTTC TAGCAACTTC 
ACAAGTGCCA AGACTTTCAT GAAAGAAATC GTTGACAGCT TTACAATTTC AGAGAACAGA 
GTGCGTATTG GAGTCGCACA GTACAGTGCA AATCCCAAGA AAGAATTCTT CTTAAATGAG 
TACTACTCCA GCTCCGACAT GAAGAAACAG ATTGATAGCA TCTCCCAACT GAAAGCTACC 
ACTTACACTG GCAAAGGCCT ACGATTTGTC AAACAGTTCT TTGATCCAGC AAATGGCGGC 
CGTAAGAATG TTCCGCAGTA CCTGATAGTC ATGACTGATG GCATGTCTAA TGACAGCGTA 
AATGAAGATG CTGCTGCTTT GCGAAGCAGT GGAGTAAAGA TCTTTTCCAT TGGTATTGGT 
CTGCGGAACA GTTTTGAGCT TGTTATGATT GCCGGGTCAC CCAAAAATGT TTACGAAGTG 
GAAACGTTCC AAGCGCTGGA CTCTATAAAG AGGCAGATTG TGGCACAAGT CTGTGAGCCA 
TCTGATCAGC CTATTATAGG TAAAGAATGC AGCTTAGATA TCACCATATC CGTTGACTAC 
TCAAGAAGAG CAATGCTATC TACTGTCCCC CGTGTGCAGC AGAAATTGCA GCAGTTCCTC 
CCTCCGGTGC TTCAGCGCAT TTCCTCGCTT AGCAATATCA GCTGTGCCTC TGGATCTCAA 
ATTAACTTAC GCTTCAGATA CGCTGTTGCA GCACAGGACA GACTGACATT TGATTCCGAC 
TTTGAGAAAT TTAACGAGGA CATCATTAAG AAGTTTATAG CTGAACAGTC TTCTGTTGAT 
ACATATTTAA ATGCAAAGTT TCTTCAGTCC CTCTGGAACA AATACATAGG TTTGCCGTCT 
CAGAAAGCAA AAATTATTCT GGTTTTTACT GATGGACTGG ATGAAAGTTC TGAAGTACTC 
AAGGCGACTA CTGAATCTCT TCGGAAACAA GGGTTGGATG CACTGTTATT CATTGCTCTT 
GAGGGGGTGA AGGATTTTAC AGAGCTGCAA AACTTAGAGT TTGGAAGAGG ATTTTCTTAC 
CAGCAACCTC TGAGCATTGC GCTGCATGAT CTGCCAAGTG TAATGCTAAA AGAAATGGAT 
ACCGTTGCAG AAAGGAAATG CTGTAATGTA GTTTGTAAAT GTTTAGGACA AGATGGACCT 
CTAGGCTCGC CAGGGGCTTT AGGAACAAAG GGTCAGCCTG GTATAAAGGG ATCTCAAGGT 
CATCCTGGAG AGGAAGGCGG TCATGGTGAA AGAGGATTCC GTGGGCTGAA TGGGACAAGA 
GGCGAAAGTG GATGTCCTGG TCGTAGGGGA TTAAAGGGTG CCAGAGGTAT TCGTGGTGAC 
AAGGGTGATG ATGGAGAGTT TGGAATTGAT GGTGTGCCTG GAGAGCAGGG TGAACCTGGA 
GGGCGTGGAG CATCAGGAGA ACGTGGAGAC ACTGGAGCTC AGGGCAGAAA AGGTCCTCGT 
GGGCAACCTG GAGAAAAAGG GGAAAATGGA CTGAGGGGAG ACCCAGGTGA GCCTGGAACT 
AACATTAACA TTCAGGGACC TAAAGGACTG AAAGGAAACC CAGGACGTCA GGGAGAGGCG 
GGAAGTGACG GTGTGGAAGG AGAGCCAGGA AATAATGGAC CTAATGGTCC ACAAGGCCGA 
AGAGGGCCTC CTGGTTTAAA GGGAGCACGA GGATTTCCTG GAGAGCCAGG GAACAAAGGT 
GATAGTGGAA TCCAAGGTAT ACAGGGTTCC CAAGGCATGC CTGGACCACC AGGTCCACAG 
GGACCACAAG GGTTATCTGG ACGTCAGGGA GATGCAGGTG CCCCTGGTGC ATCAGGTTCC 
TTTGGCAAAC CCGGAGCTCC AGGACTAAAG GGGGAACCTG GAGAAAACGG AGAGAAGGGT 
GAGCCAGGCA TTCCGGGTCG CAGAGGTTTG CCAGGGCTGG ATGGTCCTGA TGGATATGGT 
CCCCCAGGCT TAAAGGGAGA GAAGGGTCAT TTAGGGTTTC CTGGGGATCC TGGGCCTCAG 
GGAGAAGATG GTGATCCTGG AATAGCTGGA AATGGTGGCC CAAAAGGAAT TAGTGGAAGA 
AGGGGTAATT CTGGCCCTCC AGGAACTAAT GGACAAAGGG GTGAGAGGGG ACCACGTGGA 
CCTATGGGCG CCAAAGGATT GCCAGGAACT ATGCCTATGA CGCCTTGTGA ACTTGTGGCA 
TTTACTCGAG AGAATTGTCC TTGCTTCGCA AGTGCTTCAA AATGTCCAGT TTACCCAACT 
GAAATAGTGT TTGCTCTGGA TGCATCTCAG GACGTTCCCC CGGCTGCTTT CCAAAGGATG 
AAGAACACCA TTATTTCCAT AATAGAAAAG CTGGACATTA GTGAGAGCAA TTGCCCTAGA 
GGAGCCCGTG TTGCTGTTGT TGCGTATAAC AGTAATACCA AATACCTCAT TCGTTTCTCT 
GACTACAAAC GGAAAAGCCT ACTGCTGGAA GCAATTCGTG GCATCTCTCC AGAAAGATCA 
TCTTCAAGGA GAAATATTGG AGAAGCCATG AAGTTTGTAG GGAGGAACAT ATTCAAACGC 
ATTCGTCATG GAATCCATGT TAGAAAAGTG GCTGTGTTCT TTGCTAATGG CCCATCCCCA 
GATGCAGATG CAATAAACTC GGCAGTGCTG GAATACAGTG CTCTTGACAT TATTCCTATT 
GTGATTGCAT TTAATAATGT TCCCAATGTC GTAGCAGCTT TTTCGGCCGA CGAAACTAGA 
AGATTCCAAG TGTTTGTATG GAGTAGGCCT GAGGATCAGA GACTAGAACT TATTTCTTAT 
TGTACTCTTT GTTATGATAA GTGTACACCT GATGAAGAAT GTGAGCTGGA TCAAATGCCT 
GCAACCCTAA TAGATATGGA CATAGCTTTT GTATTAGACA GTTCACACAA TGTGCAAGCT 
GATGTCTTTG AGGAAATGAA AGACTTTGTG AGCAGTATGC TGGACACCGT CTACATATCA 
ACGCGGCCCG CAGTTTCTGA AACAGAAGCC AGAGTTGCCT TAGTACAACA GGCAGTTAAG 
GACTTTGGTC CAAATAGGAA TACTAGCCCA GTTAAAACTG AGTTCGACCT TCTTCGCTAC 
AACAGCGCAA ATATGATGAA GAGGCACATC AAACAATCTG TCAGGCAACT GAATGGTCCT 
TCTGCAGTTG GCAATTCTGT TCAGTGGGTC ATTGATAATA TCTTCTTAAA AACACCAATG 
CCAAGAAAGC ATAAAGTACT TTTCCTCATT CTTGCCAGCC CAACAAGCTA TTGGGACAGA 
GAAAAATTGA AAGAAGTCTC CAAAAAAGCT AAGTGTCAAG GATTTGCAAT ATTTGTACTG 
GGTTTTGGAA AAACGATAAG TGAGCTGGAA ATGACACAGC TGCCGAGCTT GCCACAAGAC 
CAACATTTGC TTCGCTTGAT AAGCATGTCA AAACCAGAAC TTGTCTATGC AAAGAAGTTC 
AGTCATGCCT TCTTCAATCT ACTCGACCGT GGCTTTAATC ACTACCCACC CGCAGTATTG 
CAAGCAGAGT GCCAAGGGCG TGGGGACTCA TCTTTTTCAG TTGTAAGAAG CTTCCCTGAC 
ACACGGTTGA CACAAGTTGA CACTGCTGCA GCAGAAGTTC CAGATGAGAC TATAGAAGTA 
GTGGAGGTCC TTGGTACACC TGCACCTGAA GAAATTGTGG GGAAAAAAAC AGAGAAAATA 
ATCATAAACA AAGATGTCTG TACAATGAAA CAAGAGGAAG GAAAATGTGC AGATTACATC 
CTCAAGTGGT CCTACAACCA AGGCAAAAAA GCTTGTGTGC AGTTCTGGTA CGGTGGCTGT 
GGAGGAAATG GCAATCGTTT TGACACACAG GAACAGTGTG AAGCCACCTG CATGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col6a5 ( XM_031903527.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759387.1 Xenopus tropicalis collagen type VI alpha 5 chain (col6a5), transcript variant X2, mRNA.

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CloneID	OXa59874
Clone ID Related Accession (Same CDS sequence)	XM_031903527.1 , XM_012965534.2
Accession Version	XM_031903527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-6(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTTT GGAAAGTATT TCTCGCCTGC GTGCTTGGCA GCTACTCAGT CAATGTCTCA 
GCTTCTCAGA AAACAGTGTG CACAGAGGCC AGTATTGCAG ATATTGTATT CCTTATGGAT 
GGATCCTGGA GCATCGGAAC GGAAAACTTT ATCACAATGA AGAACTTTTT GTACACTTTG 
GTTAATGGTT TTGATGTTGG CCTAGATAAG ATCCAGATTG GCCTAATCCA GTACAGTGAT 
AATGCACGTA CAGAATTCTT CCTAAACAGT TACAGTAACA AAGAAGATGT TTTGAAATAT 
ATTCAGAATC TAAAGTATAA AGGGGGTGGG ACAAAGACTG GCTTAAGTCT GGAGTTCATG 
CTGACGCAGC ACTTTAGTGA AGCTGCAGGC AGCAGAGCAG CAGAAGGTGT GCCTCAGATT 
GCAGTCGTTA TAACTGATGG ACAAGCTCAA GATAGCATCA GGGAGCCTGC CATTGCAGTG 
AAGAACGCTG GAATCATACT GTATGCCATT GGCATTAAAG ATGCAGTTTT ATCTGAGCTT 
AATGAAATAG CCAGTGATCC AGATGACAAA CATGTTTATA GCGTTGCAGA CTTTAATGCA 
CTGCAAAGTA TTTCCCAAAA CATGATCCAG GTGCTCTGCA CAACTGTAGA AGAAGCAGCA 
AGACAGACAG GTCAAATTGC TCAAGTTTGC AGAACTGCCA ATCAAGCTGA CATTGTGCTG 
CTAGTCGAAA GCACAACAAG AATGGGTGAC GCTACTTTTG AAAAAGCAAA GAATTTTCTT 
TATGATTTAG TTTCTAATTT GGATGTTGGA ATTAATAAGA TTCGCATTGG GCTTGTAACC 
TACAATGATG AGACAAATCC AGAATTTTTG CTAAACAGCT ATTCTTCGAA AACTGAAATC 
TTAGAAAGTA TTCAGAATAT GAAATATGTA GAAGGATATA CCTACACAGG GAGAGCCCTT 
GAGTATGTAA ATACAACCTA CTTTACACAG GCAGCAGGCA GTAGATTTGA AGAGAGCGTC 
GCTCAAATTC TTATCATTGT GACAGAAGGA GACTCTTCAG ATACCCTCAC TGAGCCTGCT 
AAAGAACTCA AATCAAGAGG CATATCTGTT TATGTGGTTG GGACAAACAT CAAGTACGAT 
AGACAGCTTC AGGAAGCATC AAGCAAGCCC GACGAAAAGT TCTTTTATCA ACTCGATGAT 
TTTGATGACT CTGAGAATGT CACTGAGCAG CTGTTAAAAA ACCTGTGCTT TAGCATAGAT 
TTGAATATTC AAGTATATTC AAAGCGCTAC GCTGATGTTG TATTTCTAGT CGATTCTTCT 
ACAAGCATGG GAACCATCTT TTTTCAAAAG ATGAAGGATT TCATCATACA CATAATAAAC 
CAGTTAAATG TTGGAATAAA TAAACACCGT ATAGGACTGG CACAGTACAG TGGTCTACCC 
CAAACTGAAT TTCTACTGAA TCATTATGAA ACCAAAGAGG AGATACTAAA GCACATTAAG 
GAAACATTCA CATACAGAGG TGGCCCCTTA AAAACAGGAC ATGCTTTGGA ATTTGTACGC 
TCAACATTTT TCATAGAAGA GGCTGGGAGT AGGATTAACT ACGGCAACCC CCAGTTCTTA 
GTGGTCATAA CATCATCAAA ATCAGAGGAT GCTGTTAGAA GACATGCCGA GGAACTCAAA 
AGTGTTGGGG TCACAACCAT CTCTGTTGGC ATTGGCAATT CTGATCGAAA AGAACTGGAA 
AAAATTGCTA CTGATCCTTT TGTTTTCCAG ACCACAGGTT TACAGCACAT TTCAAATTTA 
CAGCAGGATG TGGCCAATGT TATTATAGCA GAAGACATGC TACAGTTTTC CCCAGTATCA 
GTTGTACCAG CAGTTTGCTC CAGTGCTTCT GTAGCTGACA TTGTGTTTCT AATTGATGAA 
TCTAGCAGCA TTGGCCCCAT CAACTTTCAA CTAACCAGAG TCTTTCTACA CAAGGTTGTT 
AGTGCACTGG ATATTTCCTT GAGTAATGTT AGAGTGGGCT TAGTGCTTTA CAGTGATGAG 
CCAAGACTTG AACTTAAACT GAACACTTTT AATGAAAAAT ATGAAATTTT GGACTTCATA 
ACTAAGCTGC CATACAGAGG AGGCAAAGCA CATACAGGTG CTGCGCTGGA CTTTCTAAGG 
AAAAAAATGT TCACCAAACA AAATGGCGGC CGGCCACATC AAGGAGTTCA GCAGATAGCG 
GTGGTTATGA CTAATGGGCA GTCCATGGAT AATTTTACTA AACCTGCTGC AAAACTGAGA 
CGGTCTGGCG TGGAGGTTTT TGCTGTTGGC TTTCAGAATA TCAATGATAC AGAACTGGAT 
ATCATAGCAT CTCACCCACC GCGAAAACAT GTCACAAATG TGGAATCTTT CCTGCAGCTA 
TCCAACCTTG AGTTCAGAAT ACAGAAACGA CTTTGCAATG AAATTGTTGT GAAGTCATTT 
GCAGTACCTG CAATTGCTCG TGCAGTGAAG GAAGGGTGTG TGGATACAGA AGAAGCAGAT 
ATATACTTTC TAATTGATGG TTCTGGCAGT ATATATCCAG AGGACTTTGA AGATATGAAA 
AAATTCATGA TTGAATTAAT AAGCATGTTT CAAGTTGGTG CCAACAGAGT ACGCTTTGGA 
GTTGTCCAAT ACTCAGATGT TCGTAGAACT GAATTTTTCA TCAGTGAACA CAACACCCAG 
AAAATGTTAA AGGATGCTAT TTCTCAAATA GAACAGTTGG GCGGTGGTAC CCTAACAGGA 
GAAGCTTTAA CGTCAATGAA GCAACTTTTT GTTAATGCAG CTAAAGATCG CCCTCATAAA 
GTACCCCAGT CATTGGTTGT CATTACAGAT GGAGAATCTC AAGACCGGGT CACAGAAGCA 
GCTGCAGAAA TTAGAAATGA TGGCATTACT ATATTTGCAA TTGGTGTTAA AAATGCAGTG 
GAAGAAGAGA TTAGAGATAT AGCAGGGTCA AATGAGAAGA TGTTTTTTGT TAACAACTTT 
GATTCCCTGA AGGTGATCAA GAATGATTTG GCTCGTGAAT TGTGTACACC TGAGGCATGT 
AAGAACATGA AAGCTGATAT CGTGTTTCTG GTTGATAGCT CTGCAAGCAT TAACAGTGAT 
GATTATGAAA CAATGAAAGA ATTCATGGAG TCAATGGTCA AGCAGGCAGA AATCGGCCCT 
GATAGAGTCC AGATTGGTTT AATTCAGTTT AGCTCTGAAA CAAAGGAAGA ATTTCCTCTC 
AACAGGTACA AAAGAAAGGA TGAAATACAA TCAGCAATAA GAGGCATCCA ACAACTGAGC 
CAGGGAACCC TTATGGGGGA AGCCCTTAAA TATACATTAC CTTATTTTAG TGCTTCCAAA 
GGAGGCCGTG TAAATACCAA ACAATATCTG ATCGTCATAA CAGATGGTGA GGCCCAGGAT 
GCTGTGGGAA ACCCAGCCAA AGCAATAAGG GACCATGGAG TTATCATTTA TGCCATAGGA 
GTACAACAAG CCAACAACAC ACAACTTCTG GAGATTGCTG GCAAACAAGA GCAGGTGTAT 
TATGAAGATA GCTTTGATTC CCTAGCTTTT TTGAACAAGA ACATCATGTT TGAGATTTGC 
AATCCTCAAG AATCTTGCAA AAAGACTGAG GTGGCAGACA TCATATTTCT GTTGGATGCT 
TCTGCAAGCA TAACCAGAGG AGAGTTTAGA CTCATGCAGA GATTTGTGGA GGCTGTTGTC 
AATGACTCTT TGGTTGGAAA GGATAATGTT CAATTTGGAG CAGTTGTTTA CGGCACAAAT 
CCAGCAGAAC AGTTTTCACT CAATACGTAT TCAACTAAAC TTGATATTCT TAAGGCTGTA 
TTTAGTTTAC CCCAAGTATC TGGATACACA TACACTGCAA AAGCGCTGGA ATACACTCGG 
ATACGGTTTG GTACATCATA TGGTGGAAGA CCAGGAATTT CACATATTCT AATTCTAGTC 
ACAGATGGAG CCACTACTGA AGCTGATCGC CCTAACCTAC CAATAGTATC AAAGGCTTTG 
AAGGATGATG GCATCATTGT CTTTGCAGTA GGAGTTGGCA AGGCAGTTCC TCAAGAGCTT 
CAGCAGATTG CAGGATACCC AGACAGATGG TTCTTAGTCC AGAACTACAA AGGCCTGGAC 
AATATACATG ATAATATAAC ACAGGTTGTA TGTGATGAAT CAAAACCAGC TTGCTCACAT 
GAACAGCTTG ATCTTGTTTT CCTGATTGAC GGCTCAGCAA GTATCACTTC TAGCAACTTC 
ACAAGTGCCA AGACTTTCAT GAAAGAAATC GTTGACAGCT TTACAATTTC AGAGAACAGA 
GTGCGTATTG GAGTCGCACA GTACAGTGCA AATCCCAAGA AAGAATTCTT CTTAAATGAG 
TACTACTCCA GCTCCGACAT GAAGAAACAG ATTGATAGCA TCTCCCAACT GAAAGCTACC 
ACTTACACTG GCAAAGGCCT ACGATTTGTC AAACAGTTCT TTGATCCAGC AAATGGCGGC 
CGTAAGAATG TTCCGCAGTA CCTGATAGTC ATGACTGATG GCATGTCTAA TGACAGCGTA 
AATGAAGATG CTGCTGCTTT GCGAAGCAGT GGAGTAAAGA TCTTTTCCAT TGGTATTGGT 
CTGCGGAACA GTTTTGAGCT TGTTATGATT GCCGGGTCAC CCAAAAATGT TTACGAAGTG 
GAAACGTTCC AAGCGCTGGA CTCTATAAAG AGGCAGATTG TGGCACAAGT CTGTGAGCCA 
TCTGATCAGC CTATTATAGG TAAAGAATGC AGCTTAGATA TCACCATATC CGTTGACTAC 
TCAAGAAGAG CAATGCTATC TACTGTCCCC CGTGTGCAGC AGAAATTGCA GCAGTTCCTC 
CCTCCGGTGC TTCAGCGCAT TTCCTCGCTT AGCAATATCA GCTGTGCCTC TGGATCTCAA 
ATTAACTTAC GCTTCAGATA CGCTGTTGCA GCACAGGACA GACTGACATT TGATTCCGAC 
TTTGAGAAAT TTAACGAGGA CATCATTAAG AAGTTTATAG CTGAACAGTC TTCTGTTGAT 
ACATATTTAA ATGCAAAGTT TCTTCAGTCC CTCTGGAACA AATACATAGG TTTGCCGTCT 
CAGAAAGCAA AAATTATTCT GGTTTTTACT GATGGACTGG ATGAAAGTTC TGAAGTACTC 
AAGGCGACTA CTGAATCTCT TCGGAAACAA GGGTTGGATG CACTGTTATT CATTGCTCTT 
GAGGGGGTGA AGGATTTTAC AGAGCTGCAA AACTTAGAGT TTGGAAGAGG ATTTTCTTAC 
CAGCAACCTC TGAGCATTGC GCTGCATGAT CTGCCAAGTG TAATGCTAAA AGAAATGGAT 
ACCGTTGCAG AAAGGAAATG CTGTAATGTA GTTTGTAAAT GTTTAGGACA AGATGGACCT 
CTAGGCTCAC CAGGGGCTTT AGGAACAAAG GGTCAGCCTG GTATAAAGGG ATCTCAAGGT 
CATCCTGGAG AGGAAGGCGG TCATGGTGAA AGAGGATTCC GTGGGCTGAA TGGGACAAGA 
GGCGAAAGTG GATGTCCTGG TCGTAGGGGA TTAAAGGGTG CCAGAGGTAT TCGTGGTGAC 
AAGGGTGATG ATGGAGAGTT TGGAATTGAT GGTGTGCCTG GAGAGCAGGG TGAACCTGGA 
GGGCGTGGAG CATCAGGAGA ACGTGGAGAC ACTGGAGCTC AGGGCAGAAA AGGTCCTCGT 
GGGCAACCTG GAGAAAAAGG GGAAAATGGA CTGAGGGGAG ACCCAGGTGA GCCTGGAACT 
AACATTAACA TTCAGGGACC TATAGGACTG AAAGGAAACC CAGGACGTCA GGGAGAGGCG 
GGAAGTGACG GTGTGGAAGG AGAGCCAGGA AATAATGGAC CTAATGGTCC ACAAGGCCGA 
AGAGGGCCTC CTGGTTTAAA GGGAGCACGA GGATTTCCTG GAGAGCCAGG GAACAAAGGT 
GATAGTGGAA TCCAAGGTAT ACAGGGTTCC CAAGGCATGC CTGGACCACC AGGTCCACAG 
GGACCACAAG GGTTATCTGG ACGTCAGGGA GATGCAGGTG CCCCTGGTGC ATCAGGTTCC 
TTTGGCAAAC CCGGAGCTCC AGGACTAAAG GGGGAACCTG GAGAAAACGG AGAGAAGGGT 
GAGCCAGGCA TTCCGGGTCG CAGAGGTTTG CCAGGGCTGG ATGGTCCTGA TGGATATGGT 
CCCCCAGGCT TAAAGGGAGA GAAGGGTCAT TTAGGGTTTC CTGGGGATCC TGGGCCTCAG 
GGAGAAGATG GTGATCCTGG AATAGCTGGA AATGGTGGCC CAAAAGGAAT TAGTGGAAGA 
AGGGGTAATT CTGGCCCTCC AGGAACTAAT GGACAAAGGG GTGAGAGGGG ACCACGTGGA 
CCTATGGGCG CCAAAGGATT GCCAGGAACT ATGCCTATGA CGCCTTGTGA ACTTGTGGCG 
TTTACTCGAG AGAATTGTCC TTGCTTCGCA AGTGCTTCAA AATGTCCAGT TTACCCAACT 
GAAATAGTGT TTGCTCTGGA TGCATCTCAG GACGTTCCCC CGGCTGCTTT CCAAAGGATG 
AAGAACACCA TTATTTCCAT AATAGAAAAG CTGGACATTA GTGAGAGCAA TTGCCCTAGA 
GGAGCCCGTG TTGCTGTCGT TGCGTATAAC AGTAATACCA AATACCTCAT TCGTTTCTCT 
GACTACAAAC GGAAAAGCCT ACTGCTGGAA GCAATTCGTG GCATCTCTCC AGAAAGATCA 
TCTTCAAGGA GAAATATTGG AGAAGCCATG AAGTTTGTAG GGAGGAACAT ATTCAAACGC 
ATTCGTCATG GAATCCATGT TAGAAAAGTG GCTGTGTTCT TTGCTAATGG CCCATCCCCA 
GATGCAGATG CAATAAACTC GGCAGTGCTG GAATACAGTG CTCTTGACAT TATTCCTATT 
GTGATTGCAT TTAATAATGT TCCCAATGTC GTAGCAGCTT TTTCGGCCGA CGAAACTAGA 
AGATTCCAAG TGTTTGTATG GAGTAGGCCT GAGGATCAGA GACTAGAACT TATTTCTTAT 
TGTACTCTTT GTTATGATAA GTGTACACCT GATGAAGAAT GTGAGCTGGA TCAAATGCCT 
GCAACCCTAA TAGATATGGA CATAGCTTTT GTATTAGACA GTTCACACAA TGTGCAAGCT 
GATGTCTTTG AGGAAATGAA AGACTTTGTG AGCAGTATGC TGGACACCGT CTACATATCA 
ACGCGGCCCG CAGTTTCTGA AACAGAAGCC AGAGTTGCCT TAGTACAACA GGCAGTTAAG 
GACTTTGGTC CAAATAGGAA TACTAGCCCA GTTAAAACTG AGTTCGACCT TCTTCGCTAC 
AACAGCGCAA ATATGATGAA GAGGCACATC AAACAATCTG TCAGGCAACT GAATGGTCCT 
TCTGCAGTTG GCAATTCTGT TCAGTGGGTC ATTGATAATA TCTTCTTAAA AACACCAATG 
CCAAGAAAGC ATAAAGTACT TTTCCTCATT CTTGCCAGCC CAACAAGCTA TTGGGACAGA 
GAAAAATTGA AAGAAGTCTC CAAAAAAGCT AAGTGTCAAG GATTTGCAAT ATTTGTACTG 
GGTTTTGGAA AAACGATAAG TGAGCTGGAA ATGACACAGC TGCCGAGCTT GCCACAAGAC 
CAACATTTGC TTCGCTTGAT AAGCATGTCA AAACCAGAAC TTGTCTATGC AAAGAAGTTC 
AGTCATGCCT TCTTCAATCT ACTCGACCGT GGCTTTAATC ACTACCCACC CGCAGTATTG 
CAAGCAGAGT GCCAAGGGCG TGGGGACTCA TCTTTTTCAG TTGTAAGAAG CTTCCCTGAC 
ACACGGTTGA CACAAGTTGA CACTGCTGCA GCAGAAGTTC CAGATGAGAC TATAGAAGTA 
GTGGAGGTCC TTGGTACACC TGCACCTGAA GAAATTGTGG GGAAAAAAAC AGAGAAAATA 
ATCATAAACA AAGTTGCAGA CAACTGCTTA TTGGATGTGA ACCATGGCAC AGCGTGCAGG 
GGCTATCAGC GCATGTGGTA TTATATAAAA TCTATTGATG CATGCTCCCA GTTCTGGTAT 
GGAGGTTGTG ATGGTAATGG AAACAGATTT AGCTCTGAAA CTGAGTGTCT GCAAACTTGC 
TCTTCCAAAA GTAAAAAAGC TAATGGGGAA GTGCGGAGCC TTAAGAAAGA TGTCTGTACA 
ATGAAACAAG AGGAAGGAAA ATGTGCAGAT TACATCCTCA AGTGGTCCTA CAACCAAGGC 
AAAAAAGCTT GTGTGCAGTT CTGGTACGGT GGCTGTGGAG GAAATGGCAA TCGTTTTGAC 
ACACAGGAAC AGTGTGAAGC CACCTGCATG TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759387.1
CDS	196..8511
Translation	MGFWKVFLACVLGSYSVNVSASQKTVCTEASIADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREPAIAVKNAGIILYAIGIKDAVLSELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEPAKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRRHAEELKSVGVTTISVGIGNSDRKELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKPAAKLRRSGVEVFAVGFQNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELCTPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNPAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNPQESCKKTEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVPQELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVNEDAAALRSSGVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQVCEPSDQPIIGKECSLDITISVDYSRRAMLSTVPRVQQKLQQFLPPVLQRISSLSNISCASGSQINLRFRYAVAAQDRLTFDSDFEKFNEDIIKKFIAEQSSVDTYLNAKFLQSLWNKYIGLPSQKAKIILVFTDGLDESSEVLKATTESLRKQGLDALLFIALEGVKDFTELQNLEFGRGFSYQQPLSIALHDLPSVMLKEMDTVAERKCCNVVCKCLGQDGPLGSPGALGTKGQPGIKGSQGHPGEEGGHGERGFRGLNGTRGESGCPGRRGLKGARGIRGDKGDDGEFGIDGVPGEQGEPGGRGASGERGDTGAQGRKGPRGQPGEKGENGLRGDPGEPGTNINIQGPIGLKGNPGRQGEAGSDGVEGEPGNNGPNGPQGRRGPPGLKGARGFPGEPGNKGDSGIQGIQGSQGMPGPPGPQGPQGLSGRQGDAGAPGASGSFGKPGAPGLKGEPGENGEKGEPGIPGRRGLPGLDGPDGYGPPGLKGEKGHLGFPGDPGPQGEDGDPGIAGNGGPKGISGRRGNSGPPGTNGQRGERGPRGPMGAKGLPGTMPMTPCELVAFTRENCPCFASASKCPVYPTEIVFALDASQDVPPAAFQRMKNTIISIIEKLDISESNCPRGARVAVVAYNSNTKYLIRFSDYKRKSLLLEAIRGISPERSSSRRNIGEAMKFVGRNIFKRIRHGIHVRKVAVFFANGPSPDADAINSAVLEYSALDIIPIVIAFNNVPNVVAAFSADETRRFQVFVWSRPEDQRLELISYCTLCYDKCTPDEECELDQMPATLIDMDIAFVLDSSHNVQADVFEEMKDFVSSMLDTVYISTRPAVSETEARVALVQQAVKDFGPNRNTSPVKTEFDLLRYNSANMMKRHIKQSVRQLNGPSAVGNSVQWVIDNIFLKTPMPRKHKVLFLILASPTSYWDREKLKEVSKKAKCQGFAIFVLGFGKTISELEMTQLPSLPQDQHLLRLISMSKPELVYAKKFSHAFFNLLDRGFNHYPPAVLQAECQGRGDSSFSVVRSFPDTRLTQVDTAAAEVPDETIEVVEVLGTPAPEEIVGKKTEKIIINKVADNCLLDVNHGTACRGYQRMWYYIKSIDACSQFWYGGCDGNGNRFSSETECLQTCSSKSKKANGEVRSLKKDVCTMKQEEGKCADYILKWSYNQGKKACVQFWYGGCGGNGNRFDTQEQCEATCMS

Target ORF information:

RefSeq Version	XM_031903527.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen type VI alpha 5 chain (col6a5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903527.1

ORF Insert Sequence:

ATGGGATTTT GGAAAGTATT TCTCGCCTGC GTGCTTGGCA GCTACTCAGT CAATGTCTCA 
GCTTCTCAGA AAACAGTGTG CACAGAGGCC AGTATTGCAG ATATTGTATT CCTTATGGAT 
GGATCCTGGA GCATCGGAAC GGAAAACTTT ATCACAATGA AGAACTTTTT GTACACTTTG 
GTTAATGGTT TTGATGTTGG CCTAGATAAG ATCCAGATTG GCCTAATCCA GTACAGTGAT 
AATGCACGTA CAGAATTCTT CCTAAACAGT TACAGTAACA AAGAAGATGT TTTGAAATAT 
ATTCAGAATC TAAAGTATAA AGGGGGTGGG ACAAAGACTG GCTTAAGTCT GGAGTTCATG 
CTGACGCAGC ACTTTAGTGA AGCTGCAGGC AGCAGAGCAG CAGAAGGTGT GCCTCAGATT 
GCAGTCGTTA TAACTGATGG ACAAGCTCAA GATAGCATCA GGGAGCCTGC CATTGCAGTG 
AAGAACGCTG GAATCATACT GTATGCCATT GGCATTAAAG ATGCAGTTTT ATCTGAGCTT 
AATGAAATAG CCAGTGATCC AGATGACAAA CATGTTTATA GCGTTGCAGA CTTTAATGCA 
CTGCAAAGTA TTTCCCAAAA CATGATCCAG GTGCTCTGCA CAACTGTAGA AGAAGCAGCA 
AGACAGACAG GTCAAATTGC TCAAGTTTGC AGAACTGCCA ATCAAGCTGA CATTGTGCTG 
CTAGTCGAAA GCACAACAAG AATGGGTGAC GCTACTTTTG AAAAAGCAAA GAATTTTCTT 
TATGATTTAG TTTCTAATTT GGATGTTGGA ATTAATAAGA TTCGCATTGG GCTTGTAACC 
TACAATGATG AGACAAATCC AGAATTTTTG CTAAACAGCT ATTCTTCGAA AACTGAAATC 
TTAGAAAGTA TTCAGAATAT GAAATATGTA GAAGGATATA CCTACACAGG GAGAGCCCTT 
GAGTATGTAA ATACAACCTA CTTTACACAG GCAGCAGGCA GTAGATTTGA AGAGAGCGTC 
GCTCAAATTC TTATCATTGT GACAGAAGGA GACTCTTCAG ATACCCTCAC TGAGCCTGCT 
AAAGAACTCA AATCAAGAGG CATATCTGTT TATGTGGTTG GGACAAACAT CAAGTACGAT 
AGACAGCTTC AGGAAGCATC AAGCAAGCCC GACGAAAAGT TCTTTTATCA ACTCGATGAT 
TTTGATGACT CTGAGAATGT CACTGAGCAG CTGTTAAAAA ACCTGTGCTT TAGCATAGAT 
TTGAATATTC AAGTATATTC AAAGCGCTAC GCTGATGTTG TATTTCTAGT CGATTCTTCT 
ACAAGCATGG GAACCATCTT TTTTCAAAAG ATGAAGGATT TCATCATACA CATAATAAAC 
CAGTTAAATG TTGGAATAAA TAAACACCGT ATAGGACTGG CACAGTACAG TGGTCTACCC 
CAAACTGAAT TTCTACTGAA TCATTATGAA ACCAAAGAGG AGATACTAAA GCACATTAAG 
GAAACATTCA CATACAGAGG TGGCCCCTTA AAAACAGGAC ATGCTTTGGA ATTTGTACGC 
TCAACATTTT TCATAGAAGA GGCTGGGAGT AGGATTAACT ACGGCAACCC CCAGTTCTTA 
GTGGTCATAA CATCATCAAA ATCAGAGGAT GCTGTTAGAA GACATGCCGA GGAACTCAAA 
AGTGTTGGGG TCACAACCAT CTCTGTTGGC ATTGGCAATT CTGATCGAAA AGAACTGGAA 
AAAATTGCTA CTGATCCTTT TGTTTTCCAG ACCACAGGTT TACAGCACAT TTCAAATTTA 
CAGCAGGATG TGGCCAATGT TATTATAGCA GAAGACATGC TACAGTTTTC CCCAGTATCA 
GTTGTACCAG CAGTTTGCTC CAGTGCTTCT GTAGCTGACA TTGTGTTTCT AATTGATGAA 
TCTAGCAGCA TTGGCCCCAT CAACTTTCAA CTAACCAGAG TCTTTCTACA CAAGGTTGTT 
AGTGCACTGG ATATTTCCTT GAGTAATGTT AGAGTGGGCT TAGTGCTTTA CAGTGATGAG 
CCAAGACTTG AACTTAAACT GAACACTTTT AATGAAAAAT ATGAAATTTT GGACTTCATA 
ACTAAGCTGC CATACAGAGG AGGCAAAGCA CATACAGGTG CTGCGCTGGA CTTTCTAAGG 
AAAAAAATGT TCACCAAACA AAATGGCGGC CGGCCACATC AAGGAGTTCA GCAGATAGCG 
GTGGTTATGA CTAATGGGCA GTCCATGGAT AATTTTACTA AACCTGCTGC AAAACTGAGA 
CGGTCTGGCG TGGAGGTTTT TGCTGTTGGC TTTCAGAATA TCAATGATAC AGAACTGGAT 
ATCATAGCAT CTCACCCACC GCGAAAACAT GTCACAAATG TGGAATCTTT CCTGCAGCTA 
TCCAACCTTG AGTTCAGAAT ACAGAAACGA CTTTGCAATG AAATTGTTGT GAAGTCATTT 
GCAGTACCTG CAATTGCTCG TGCAGTGAAG GAAGGGTGTG TGGATACAGA AGAAGCAGAT 
ATATACTTTC TAATTGATGG TTCTGGCAGT ATATATCCAG AGGACTTTGA AGATATGAAA 
AAATTCATGA TTGAATTAAT AAGCATGTTT CAAGTTGGTG CCAACAGAGT ACGCTTTGGA 
GTTGTCCAAT ACTCAGATGT TCGTAGAACT GAATTTTTCA TCAGTGAACA CAACACCCAG 
AAAATGTTAA AGGATGCTAT TTCTCAAATA GAACAGTTGG GCGGTGGTAC CCTAACAGGA 
GAAGCTTTAA CGTCAATGAA GCAACTTTTT GTTAATGCAG CTAAAGATCG CCCTCATAAA 
GTACCCCAGT CATTGGTTGT CATTACAGAT GGAGAATCTC AAGACCGGGT CACAGAAGCA 
GCTGCAGAAA TTAGAAATGA TGGCATTACT ATATTTGCAA TTGGTGTTAA AAATGCAGTG 
GAAGAAGAGA TTAGAGATAT AGCAGGGTCA AATGAGAAGA TGTTTTTTGT TAACAACTTT 
GATTCCCTGA AGGTGATCAA GAATGATTTG GCTCGTGAAT TGTGTACACC TGAGGCATGT 
AAGAACATGA AAGCTGATAT CGTGTTTCTG GTTGATAGCT CTGCAAGCAT TAACAGTGAT 
GATTATGAAA CAATGAAAGA ATTCATGGAG TCAATGGTCA AGCAGGCAGA AATCGGCCCT 
GATAGAGTCC AGATTGGTTT AATTCAGTTT AGCTCTGAAA CAAAGGAAGA ATTTCCTCTC 
AACAGGTACA AAAGAAAGGA TGAAATACAA TCAGCAATAA GAGGCATCCA ACAACTGAGC 
CAGGGAACCC TTATGGGGGA AGCCCTTAAA TATACATTAC CTTATTTTAG TGCTTCCAAA 
GGAGGCCGTG TAAATACCAA ACAATATCTG ATCGTCATAA CAGATGGTGA GGCCCAGGAT 
GCTGTGGGAA ACCCAGCCAA AGCAATAAGG GACCATGGAG TTATCATTTA TGCCATAGGA 
GTACAACAAG CCAACAACAC ACAACTTCTG GAGATTGCTG GCAAACAAGA GCAGGTGTAT 
TATGAAGATA GCTTTGATTC CCTAGCTTTT TTGAACAAGA ACATCATGTT TGAGATTTGC 
AATCCTCAAG AATCTTGCAA AAAGACTGAG GTGGCAGACA TCATATTTCT GTTGGATGCT 
TCTGCAAGCA TAACCAGAGG AGAGTTTAGA CTCATGCAGA GATTTGTGGA GGCTGTTGTC 
AATGACTCTT TGGTTGGAAA GGATAATGTT CAATTTGGAG CAGTTGTTTA CGGCACAAAT 
CCAGCAGAAC AGTTTTCACT CAATACGTAT TCAACTAAAC TTGATATTCT TAAGGCTGTA 
TTTAGTTTAC CCCAAGTATC TGGATACACA TACACTGCAA AAGCGCTGGA ATACACTCGG 
ATACGGTTTG GTACATCATA TGGTGGAAGA CCAGGAATTT CACATATTCT AATTCTAGTC 
ACAGATGGAG CCACTACTGA AGCTGATCGC CCTAACCTAC CAATAGTATC AAAGGCTTTG 
AAGGATGATG GCATCATTGT CTTTGCAGTA GGAGTTGGCA AGGCAGTTCC TCAAGAGCTT 
CAGCAGATTG CAGGATACCC AGACAGATGG TTCTTAGTCC AGAACTACAA AGGCCTGGAC 
AATATACATG ATAATATAAC ACAGGTTGTA TGTGATGAAT CAAAACCAGC TTGCTCACAT 
GAACAGCTTG ATCTTGTTTT CCTGATTGAC GGCTCAGCAA GTATCACTTC TAGCAACTTC 
ACAAGTGCCA AGACTTTCAT GAAAGAAATC GTTGACAGCT TTACAATTTC AGAGAACAGA 
GTGCGTATTG GAGTCGCACA GTACAGTGCA AATCCCAAGA AAGAATTCTT CTTAAATGAG 
TACTACTCCA GCTCCGACAT GAAGAAACAG ATTGATAGCA TCTCCCAACT GAAAGCTACC 
ACTTACACTG GCAAAGGCCT ACGATTTGTC AAACAGTTCT TTGATCCAGC AAATGGCGGC 
CGTAAGAATG TTCCGCAGTA CCTGATAGTC ATGACTGATG GCATGTCTAA TGACAGCGTA 
AATGAAGATG CTGCTGCTTT GCGAAGCAGT GGAGTAAAGA TCTTTTCCAT TGGTATTGGT 
CTGCGGAACA GTTTTGAGCT TGTTATGATT GCCGGGTCAC CCAAAAATGT TTACGAAGTG 
GAAACGTTCC AAGCGCTGGA CTCTATAAAG AGGCAGATTG TGGCACAAGT CTGTGAGCCA 
TCTGATCAGC CTATTATAGG TAAAGAATGC AGCTTAGATA TCACCATATC CGTTGACTAC 
TCAAGAAGAG CAATGCTATC TACTGTCCCC CGTGTGCAGC AGAAATTGCA GCAGTTCCTC 
CCTCCGGTGC TTCAGCGCAT TTCCTCGCTT AGCAATATCA GCTGTGCCTC TGGATCTCAA 
ATTAACTTAC GCTTCAGATA CGCTGTTGCA GCACAGGACA GACTGACATT TGATTCCGAC 
TTTGAGAAAT TTAACGAGGA CATCATTAAG AAGTTTATAG CTGAACAGTC TTCTGTTGAT 
ACATATTTAA ATGCAAAGTT TCTTCAGTCC CTCTGGAACA AATACATAGG TTTGCCGTCT 
CAGAAAGCAA AAATTATTCT GGTTTTTACT GATGGACTGG ATGAAAGTTC TGAAGTACTC 
AAGGCGACTA CTGAATCTCT TCGGAAACAA GGGTTGGATG CACTGTTATT CATTGCTCTT 
GAGGGGGTGA AGGATTTTAC AGAGCTGCAA AACTTAGAGT TTGGAAGAGG ATTTTCTTAC 
CAGCAACCTC TGAGCATTGC GCTGCATGAT CTGCCAAGTG TAATGCTAAA AGAAATGGAT 
ACCGTTGCAG AAAGGAAATG CTGTAATGTA GTTTGTAAAT GTTTAGGACA AGATGGACCT 
CTAGGCTCAC CAGGGGCTTT AGGAACAAAG GGTCAGCCTG GTATAAAGGG ATCTCAAGGT 
CATCCTGGAG AGGAAGGCGG TCATGGTGAA AGAGGATTCC GTGGGCTGAA TGGGACAAGA 
GGCGAAAGTG GATGTCCTGG TCGTAGGGGA TTAAAGGGTG CCAGAGGTAT TCGTGGTGAC 
AAGGGTGATG ATGGAGAGTT TGGAATTGAT GGTGTGCCTG GAGAGCAGGG TGAACCTGGA 
GGGCGTGGAG CATCAGGAGA ACGTGGAGAC ACTGGAGCTC AGGGCAGAAA AGGTCCTCGT 
GGGCAACCTG GAGAAAAAGG GGAAAATGGA CTGAGGGGAG ACCCAGGTGA GCCTGGAACT 
AACATTAACA TTCAGGGACC TATAGGACTG AAAGGAAACC CAGGACGTCA GGGAGAGGCG 
GGAAGTGACG GTGTGGAAGG AGAGCCAGGA AATAATGGAC CTAATGGTCC ACAAGGCCGA 
AGAGGGCCTC CTGGTTTAAA GGGAGCACGA GGATTTCCTG GAGAGCCAGG GAACAAAGGT 
GATAGTGGAA TCCAAGGTAT ACAGGGTTCC CAAGGCATGC CTGGACCACC AGGTCCACAG 
GGACCACAAG GGTTATCTGG ACGTCAGGGA GATGCAGGTG CCCCTGGTGC ATCAGGTTCC 
TTTGGCAAAC CCGGAGCTCC AGGACTAAAG GGGGAACCTG GAGAAAACGG AGAGAAGGGT 
GAGCCAGGCA TTCCGGGTCG CAGAGGTTTG CCAGGGCTGG ATGGTCCTGA TGGATATGGT 
CCCCCAGGCT TAAAGGGAGA GAAGGGTCAT TTAGGGTTTC CTGGGGATCC TGGGCCTCAG 
GGAGAAGATG GTGATCCTGG AATAGCTGGA AATGGTGGCC CAAAAGGAAT TAGTGGAAGA 
AGGGGTAATT CTGGCCCTCC AGGAACTAAT GGACAAAGGG GTGAGAGGGG ACCACGTGGA 
CCTATGGGCG CCAAAGGATT GCCAGGAACT ATGCCTATGA CGCCTTGTGA ACTTGTGGCG 
TTTACTCGAG AGAATTGTCC TTGCTTCGCA AGTGCTTCAA AATGTCCAGT TTACCCAACT 
GAAATAGTGT TTGCTCTGGA TGCATCTCAG GACGTTCCCC CGGCTGCTTT CCAAAGGATG 
AAGAACACCA TTATTTCCAT AATAGAAAAG CTGGACATTA GTGAGAGCAA TTGCCCTAGA 
GGAGCCCGTG TTGCTGTCGT TGCGTATAAC AGTAATACCA AATACCTCAT TCGTTTCTCT 
GACTACAAAC GGAAAAGCCT ACTGCTGGAA GCAATTCGTG GCATCTCTCC AGAAAGATCA 
TCTTCAAGGA GAAATATTGG AGAAGCCATG AAGTTTGTAG GGAGGAACAT ATTCAAACGC 
ATTCGTCATG GAATCCATGT TAGAAAAGTG GCTGTGTTCT TTGCTAATGG CCCATCCCCA 
GATGCAGATG CAATAAACTC GGCAGTGCTG GAATACAGTG CTCTTGACAT TATTCCTATT 
GTGATTGCAT TTAATAATGT TCCCAATGTC GTAGCAGCTT TTTCGGCCGA CGAAACTAGA 
AGATTCCAAG TGTTTGTATG GAGTAGGCCT GAGGATCAGA GACTAGAACT TATTTCTTAT 
TGTACTCTTT GTTATGATAA GTGTACACCT GATGAAGAAT GTGAGCTGGA TCAAATGCCT 
GCAACCCTAA TAGATATGGA CATAGCTTTT GTATTAGACA GTTCACACAA TGTGCAAGCT 
GATGTCTTTG AGGAAATGAA AGACTTTGTG AGCAGTATGC TGGACACCGT CTACATATCA 
ACGCGGCCCG CAGTTTCTGA AACAGAAGCC AGAGTTGCCT TAGTACAACA GGCAGTTAAG 
GACTTTGGTC CAAATAGGAA TACTAGCCCA GTTAAAACTG AGTTCGACCT TCTTCGCTAC 
AACAGCGCAA ATATGATGAA GAGGCACATC AAACAATCTG TCAGGCAACT GAATGGTCCT 
TCTGCAGTTG GCAATTCTGT TCAGTGGGTC ATTGATAATA TCTTCTTAAA AACACCAATG 
CCAAGAAAGC ATAAAGTACT TTTCCTCATT CTTGCCAGCC CAACAAGCTA TTGGGACAGA 
GAAAAATTGA AAGAAGTCTC CAAAAAAGCT AAGTGTCAAG GATTTGCAAT ATTTGTACTG 
GGTTTTGGAA AAACGATAAG TGAGCTGGAA ATGACACAGC TGCCGAGCTT GCCACAAGAC 
CAACATTTGC TTCGCTTGAT AAGCATGTCA AAACCAGAAC TTGTCTATGC AAAGAAGTTC 
AGTCATGCCT TCTTCAATCT ACTCGACCGT GGCTTTAATC ACTACCCACC CGCAGTATTG 
CAAGCAGAGT GCCAAGGGCG TGGGGACTCA TCTTTTTCAG TTGTAAGAAG CTTCCCTGAC 
ACACGGTTGA CACAAGTTGA CACTGCTGCA GCAGAAGTTC CAGATGAGAC TATAGAAGTA 
GTGGAGGTCC TTGGTACACC TGCACCTGAA GAAATTGTGG GGAAAAAAAC AGAGAAAATA 
ATCATAAACA AAGTTGCAGA CAACTGCTTA TTGGATGTGA ACCATGGCAC AGCGTGCAGG 
GGCTATCAGC GCATGTGGTA TTATATAAAA TCTATTGATG CATGCTCCCA GTTCTGGTAT 
GGAGGTTGTG ATGGTAATGG AAACAGATTT AGCTCTGAAA CTGAGTGTCT GCAAACTTGC 
TCTTCCAAAA GTAAAAAAGC TAATGGGGAA GTGCGGAGCC TTAAGAAAGA TGTCTGTACA 
ATGAAACAAG AGGAAGGAAA ATGTGCAGAT TACATCCTCA AGTGGTCCTA CAACCAAGGC 
AAAAAAGCTT GTGTGCAGTT CTGGTACGGT GGCTGTGGAG GAAATGGCAA TCGTTTTGAC 
ACACAGGAAC AGTGTGAAGC CACCTGCATG TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col6a5 ( XM_012965534.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820988.2 Xenopus tropicalis collagen type VI alpha 5 chain (col6a5), transcript variant X1, mRNA.

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CloneID	OXa59874
Clone ID Related Accession (Same CDS sequence)	XM_031903527.1 , XM_012965534.2
Accession Version	XM_012965534.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-6(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965534.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTTT GGAAAGTATT TCTCGCCTGC GTGCTTGGCA GCTACTCAGT CAATGTCTCA 
GCTTCTCAGA AAACAGTGTG CACAGAGGCC AGTATTGCAG ATATTGTATT CCTTATGGAT 
GGATCCTGGA GCATCGGAAC GGAAAACTTT ATCACAATGA AGAACTTTTT GTACACTTTG 
GTTAATGGTT TTGATGTTGG CCTAGATAAG ATCCAGATTG GCCTAATCCA GTACAGTGAT 
AATGCACGTA CAGAATTCTT CCTAAACAGT TACAGTAACA AAGAAGATGT TTTGAAATAT 
ATTCAGAATC TAAAGTATAA AGGGGGTGGG ACAAAGACTG GCTTAAGTCT GGAGTTCATG 
CTGACGCAGC ACTTTAGTGA AGCTGCAGGC AGCAGAGCAG CAGAAGGTGT GCCTCAGATT 
GCAGTCGTTA TAACTGATGG ACAAGCTCAA GATAGCATCA GGGAGCCTGC CATTGCAGTG 
AAGAACGCTG GAATCATACT GTATGCCATT GGCATTAAAG ATGCAGTTTT ATCTGAGCTT 
AATGAAATAG CCAGTGATCC AGATGACAAA CATGTTTATA GCGTTGCAGA CTTTAATGCA 
CTGCAAAGTA TTTCCCAAAA CATGATCCAG GTGCTCTGCA CAACTGTAGA AGAAGCAGCA 
AGACAGACAG GTCAAATTGC TCAAGTTTGC AGAACTGCCA ATCAAGCTGA CATTGTGCTG 
CTAGTCGAAA GCACAACAAG AATGGGTGAC GCTACTTTTG AAAAAGCAAA GAATTTTCTT 
TATGATTTAG TTTCTAATTT GGATGTTGGA ATTAATAAGA TTCGCATTGG GCTTGTAACC 
TACAATGATG AGACAAATCC AGAATTTTTG CTAAACAGCT ATTCTTCGAA AACTGAAATC 
TTAGAAAGTA TTCAGAATAT GAAATATGTA GAAGGATATA CCTACACAGG GAGAGCCCTT 
GAGTATGTAA ATACAACCTA CTTTACACAG GCAGCAGGCA GTAGATTTGA AGAGAGCGTC 
GCTCAAATTC TTATCATTGT GACAGAAGGA GACTCTTCAG ATACCCTCAC TGAGCCTGCT 
AAAGAACTCA AATCAAGAGG CATATCTGTT TATGTGGTTG GGACAAACAT CAAGTACGAT 
AGACAGCTTC AGGAAGCATC AAGCAAGCCC GACGAAAAGT TCTTTTATCA ACTCGATGAT 
TTTGATGACT CTGAGAATGT CACTGAGCAG CTGTTAAAAA ACCTGTGCTT TAGCATAGAT 
TTGAATATTC AAGTATATTC AAAGCGCTAC GCTGATGTTG TATTTCTAGT CGATTCTTCT 
ACAAGCATGG GAACCATCTT TTTTCAAAAG ATGAAGGATT TCATCATACA CATAATAAAC 
CAGTTAAATG TTGGAATAAA TAAACACCGT ATAGGACTGG CACAGTACAG TGGTCTACCC 
CAAACTGAAT TTCTACTGAA TCATTATGAA ACCAAAGAGG AGATACTAAA GCACATTAAG 
GAAACATTCA CATACAGAGG TGGCCCCTTA AAAACAGGAC ATGCTTTGGA ATTTGTACGC 
TCAACATTTT TCATAGAAGA GGCTGGGAGT AGGATTAACT ACGGCAACCC CCAGTTCTTA 
GTGGTCATAA CATCATCAAA ATCAGAGGAT GCTGTTAGAA GACATGCCGA GGAACTCAAA 
AGTGTTGGGG TCACAACCAT CTCTGTTGGC ATTGGCAATT CTGATCGAAA AGAACTGGAA 
AAAATTGCTA CTGATCCTTT TGTTTTCCAG ACCACAGGTT TACAGCACAT TTCAAATTTA 
CAGCAGGATG TGGCCAATGT TATTATAGCA GAAGACATGC TACAGTTTTC CCCAGTATCA 
GTTGTACCAG CAGTTTGCTC CAGTGCTTCT GTAGCTGACA TTGTGTTTCT AATTGATGAA 
TCTAGCAGCA TTGGCCCCAT CAACTTTCAA CTAACCAGAG TCTTTCTACA CAAGGTTGTT 
AGTGCACTGG ATATTTCCTT GAGTAATGTT AGAGTGGGCT TAGTGCTTTA CAGTGATGAG 
CCAAGACTTG AACTTAAACT GAACACTTTT AATGAAAAAT ATGAAATTTT GGACTTCATA 
ACTAAGCTGC CATACAGAGG AGGCAAAGCA CATACAGGTG CTGCGCTGGA CTTTCTAAGG 
AAAAAAATGT TCACCAAACA AAATGGCGGC CGGCCACATC AAGGAGTTCA GCAGATAGCG 
GTGGTTATGA CTAATGGGCA GTCCATGGAT AATTTTACTA AACCTGCTGC AAAACTGAGA 
CGGTCTGGCG TGGAGGTTTT TGCTGTTGGC TTTCAGAATA TCAATGATAC AGAACTGGAT 
ATCATAGCAT CTCACCCACC GCGAAAACAT GTCACAAATG TGGAATCTTT CCTGCAGCTA 
TCCAACCTTG AGTTCAGAAT ACAGAAACGA CTTTGCAATG AAATTGTTGT GAAGTCATTT 
GCAGTACCTG CAATTGCTCG TGCAGTGAAG GAAGGGTGTG TGGATACAGA AGAAGCAGAT 
ATATACTTTC TAATTGATGG TTCTGGCAGT ATATATCCAG AGGACTTTGA AGATATGAAA 
AAATTCATGA TTGAATTAAT AAGCATGTTT CAAGTTGGTG CCAACAGAGT ACGCTTTGGA 
GTTGTCCAAT ACTCAGATGT TCGTAGAACT GAATTTTTCA TCAGTGAACA CAACACCCAG 
AAAATGTTAA AGGATGCTAT TTCTCAAATA GAACAGTTGG GCGGTGGTAC CCTAACAGGA 
GAAGCTTTAA CGTCAATGAA GCAACTTTTT GTTAATGCAG CTAAAGATCG CCCTCATAAA 
GTACCCCAGT CATTGGTTGT CATTACAGAT GGAGAATCTC AAGACCGGGT CACAGAAGCA 
GCTGCAGAAA TTAGAAATGA TGGCATTACT ATATTTGCAA TTGGTGTTAA AAATGCAGTG 
GAAGAAGAGA TTAGAGATAT AGCAGGGTCA AATGAGAAGA TGTTTTTTGT TAACAACTTT 
GATTCCCTGA AGGTGATCAA GAATGATTTG GCTCGTGAAT TGTGTACACC TGAGGCATGT 
AAGAACATGA AAGCTGATAT CGTGTTTCTG GTTGATAGCT CTGCAAGCAT TAACAGTGAT 
GATTATGAAA CAATGAAAGA ATTCATGGAG TCAATGGTCA AGCAGGCAGA AATCGGCCCT 
GATAGAGTCC AGATTGGTTT AATTCAGTTT AGCTCTGAAA CAAAGGAAGA ATTTCCTCTC 
AACAGGTACA AAAGAAAGGA TGAAATACAA TCAGCAATAA GAGGCATCCA ACAACTGAGC 
CAGGGAACCC TTATGGGGGA AGCCCTTAAA TATACATTAC CTTATTTTAG TGCTTCCAAA 
GGAGGCCGTG TAAATACCAA ACAATATCTG ATCGTCATAA CAGATGGTGA GGCCCAGGAT 
GCTGTGGGAA ACCCAGCCAA AGCAATAAGG GACCATGGAG TTATCATTTA TGCCATAGGA 
GTACAACAAG CCAACAACAC ACAACTTCTG GAGATTGCTG GCAAACAAGA GCAGGTGTAT 
TATGAAGATA GCTTTGATTC CCTAGCTTTT TTGAACAAGA ACATCATGTT TGAGATTTGC 
AATCCTCAAG AATCTTGCAA AAAGACTGAG GTGGCAGACA TCATATTTCT GTTGGATGCT 
TCTGCAAGCA TAACCAGAGG AGAGTTTAGA CTCATGCAGA GATTTGTGGA GGCTGTTGTC 
AATGACTCTT TGGTTGGAAA GGATAATGTT CAATTTGGAG CAGTTGTTTA CGGCACAAAT 
CCAGCAGAAC AGTTTTCACT CAATACGTAT TCAACTAAAC TTGATATTCT TAAGGCTGTA 
TTTAGTTTAC CCCAAGTATC TGGATACACA TACACTGCAA AAGCGCTGGA ATACACTCGG 
ATACGGTTTG GTACATCATA TGGTGGAAGA CCAGGAATTT CACATATTCT AATTCTAGTC 
ACAGATGGAG CCACTACTGA AGCTGATCGC CCTAACCTAC CAATAGTATC AAAGGCTTTG 
AAGGATGATG GCATCATTGT CTTTGCAGTA GGAGTTGGCA AGGCAGTTCC TCAAGAGCTT 
CAGCAGATTG CAGGATACCC AGACAGATGG TTCTTAGTCC AGAACTACAA AGGCCTGGAC 
AATATACATG ATAATATAAC ACAGGTTGTA TGTGATGAAT CAAAACCAGC TTGCTCACAT 
GAACAGCTTG ATCTTGTTTT CCTGATTGAC GGCTCAGCAA GTATCACTTC TAGCAACTTC 
ACAAGTGCCA AGACTTTCAT GAAAGAAATC GTTGACAGCT TTACAATTTC AGAGAACAGA 
GTGCGTATTG GAGTCGCACA GTACAGTGCA AATCCCAAGA AAGAATTCTT CTTAAATGAG 
TACTACTCCA GCTCCGACAT GAAGAAACAG ATTGATAGCA TCTCCCAACT GAAAGCTACC 
ACTTACACTG GCAAAGGCCT ACGATTTGTC AAACAGTTCT TTGATCCAGC AAATGGCGGC 
CGTAAGAATG TTCCGCAGTA CCTGATAGTC ATGACTGATG GCATGTCTAA TGACAGCGTA 
AATGAAGATG CTGCTGCTTT GCGAAGCAGT GGAGTAAAGA TCTTTTCCAT TGGTATTGGT 
CTGCGGAACA GTTTTGAGCT TGTTATGATT GCCGGGTCAC CCAAAAATGT TTACGAAGTG 
GAAACGTTCC AAGCGCTGGA CTCTATAAAG AGGCAGATTG TGGCACAAGT CTGTGAGCCA 
TCTGATCAGC CTATTATAGG TAAAGAATGC AGCTTAGATA TCACCATATC CGTTGACTAC 
TCAAGAAGAG CAATGCTATC TACTGTCCCC CGTGTGCAGC AGAAATTGCA GCAGTTCCTC 
CCTCCGGTGC TTCAGCGCAT TTCCTCGCTT AGCAATATCA GCTGTGCCTC TGGATCTCAA 
ATTAACTTAC GCTTCAGATA CGCTGTTGCA GCACAGGACA GACTGACATT TGATTCCGAC 
TTTGAGAAAT TTAACGAGGA CATCATTAAG AAGTTTATAG CTGAACAGTC TTCTGTTGAT 
ACATATTTAA ATGCAAAGTT TCTTCAGTCC CTCTGGAACA AATACATAGG TTTGCCGTCT 
CAGAAAGCAA AAATTATTCT GGTTTTTACT GATGGACTGG ATGAAAGTTC TGAAGTACTC 
AAGGCGACTA CTGAATCTCT TCGGAAACAA GGGTTGGATG CACTGTTATT CATTGCTCTT 
GAGGGGGTGA AGGATTTTAC AGAGCTGCAA AACTTAGAGT TTGGAAGAGG ATTTTCTTAC 
CAGCAACCTC TGAGCATTGC GCTGCATGAT CTGCCAAGTG TAATGCTAAA AGAAATGGAT 
ACCGTTGCAG AAAGGAAATG CTGTAATGTA GTTTGTAAAT GTTTAGGACA AGATGGACCT 
CTAGGCTCAC CAGGGGCTTT AGGAACAAAG GGTCAGCCTG GTATAAAGGG ATCTCAAGGT 
CATCCTGGAG AGGAAGGCGG TCATGGTGAA AGAGGATTCC GTGGGCTGAA TGGGACAAGA 
GGCGAAAGTG GATGTCCTGG TCGTAGGGGA TTAAAGGGTG CCAGAGGTAT TCGTGGTGAC 
AAGGGTGATG ATGGAGAGTT TGGAATTGAT GGTGTGCCTG GAGAGCAGGG TGAACCTGGA 
GGGCGTGGAG CATCAGGAGA ACGTGGAGAC ACTGGAGCTC AGGGCAGAAA AGGTCCTCGT 
GGGCAACCTG GAGAAAAAGG GGAAAATGGA CTGAGGGGAG ACCCAGGTGA GCCTGGAACT 
AACATTAACA TTCAGGGACC TATAGGACTG AAAGGAAACC CAGGACGTCA GGGAGAGGCG 
GGAAGTGACG GTGTGGAAGG AGAGCCAGGA AATAATGGAC CTAATGGTCC ACAAGGCCGA 
AGAGGGCCTC CTGGTTTAAA GGGAGCACGA GGATTTCCTG GAGAGCCAGG GAACAAAGGT 
GATAGTGGAA TCCAAGGTAT ACAGGGTTCC CAAGGCATGC CTGGACCACC AGGTCCACAG 
GGACCACAAG GGTTATCTGG ACGTCAGGGA GATGCAGGTG CCCCTGGTGC ATCAGGTTCC 
TTTGGCAAAC CCGGAGCTCC AGGACTAAAG GGGGAACCTG GAGAAAACGG AGAGAAGGGT 
GAGCCAGGCA TTCCGGGTCG CAGAGGTTTG CCAGGGCTGG ATGGTCCTGA TGGATATGGT 
CCCCCAGGCT TAAAGGGAGA GAAGGGTCAT TTAGGGTTTC CTGGGGATCC TGGGCCTCAG 
GGAGAAGATG GTGATCCTGG AATAGCTGGA AATGGTGGCC CAAAAGGAAT TAGTGGAAGA 
AGGGGTAATT CTGGCCCTCC AGGAACTAAT GGACAAAGGG GTGAGAGGGG ACCACGTGGA 
CCTATGGGCG CCAAAGGATT GCCAGGAACT ATGCCTATGA CGCCTTGTGA ACTTGTGGCG 
TTTACTCGAG AGAATTGTCC TTGCTTCGCA AGTGCTTCAA AATGTCCAGT TTACCCAACT 
GAAATAGTGT TTGCTCTGGA TGCATCTCAG GACGTTCCCC CGGCTGCTTT CCAAAGGATG 
AAGAACACCA TTATTTCCAT AATAGAAAAG CTGGACATTA GTGAGAGCAA TTGCCCTAGA 
GGAGCCCGTG TTGCTGTCGT TGCGTATAAC AGTAATACCA AATACCTCAT TCGTTTCTCT 
GACTACAAAC GGAAAAGCCT ACTGCTGGAA GCAATTCGTG GCATCTCTCC AGAAAGATCA 
TCTTCAAGGA GAAATATTGG AGAAGCCATG AAGTTTGTAG GGAGGAACAT ATTCAAACGC 
ATTCGTCATG GAATCCATGT TAGAAAAGTG GCTGTGTTCT TTGCTAATGG CCCATCCCCA 
GATGCAGATG CAATAAACTC GGCAGTGCTG GAATACAGTG CTCTTGACAT TATTCCTATT 
GTGATTGCAT TTAATAATGT TCCCAATGTC GTAGCAGCTT TTTCGGCCGA CGAAACTAGA 
AGATTCCAAG TGTTTGTATG GAGTAGGCCT GAGGATCAGA GACTAGAACT TATTTCTTAT 
TGTACTCTTT GTTATGATAA GTGTACACCT GATGAAGAAT GTGAGCTGGA TCAAATGCCT 
GCAACCCTAA TAGATATGGA CATAGCTTTT GTATTAGACA GTTCACACAA TGTGCAAGCT 
GATGTCTTTG AGGAAATGAA AGACTTTGTG AGCAGTATGC TGGACACCGT CTACATATCA 
ACGCGGCCCG CAGTTTCTGA AACAGAAGCC AGAGTTGCCT TAGTACAACA GGCAGTTAAG 
GACTTTGGTC CAAATAGGAA TACTAGCCCA GTTAAAACTG AGTTCGACCT TCTTCGCTAC 
AACAGCGCAA ATATGATGAA GAGGCACATC AAACAATCTG TCAGGCAACT GAATGGTCCT 
TCTGCAGTTG GCAATTCTGT TCAGTGGGTC ATTGATAATA TCTTCTTAAA AACACCAATG 
CCAAGAAAGC ATAAAGTACT TTTCCTCATT CTTGCCAGCC CAACAAGCTA TTGGGACAGA 
GAAAAATTGA AAGAAGTCTC CAAAAAAGCT AAGTGTCAAG GATTTGCAAT ATTTGTACTG 
GGTTTTGGAA AAACGATAAG TGAGCTGGAA ATGACACAGC TGCCGAGCTT GCCACAAGAC 
CAACATTTGC TTCGCTTGAT AAGCATGTCA AAACCAGAAC TTGTCTATGC AAAGAAGTTC 
AGTCATGCCT TCTTCAATCT ACTCGACCGT GGCTTTAATC ACTACCCACC CGCAGTATTG 
CAAGCAGAGT GCCAAGGGCG TGGGGACTCA TCTTTTTCAG TTGTAAGAAG CTTCCCTGAC 
ACACGGTTGA CACAAGTTGA CACTGCTGCA GCAGAAGTTC CAGATGAGAC TATAGAAGTA 
GTGGAGGTCC TTGGTACACC TGCACCTGAA GAAATTGTGG GGAAAAAAAC AGAGAAAATA 
ATCATAAACA AAGTTGCAGA CAACTGCTTA TTGGATGTGA ACCATGGCAC AGCGTGCAGG 
GGCTATCAGC GCATGTGGTA TTATATAAAA TCTATTGATG CATGCTCCCA GTTCTGGTAT 
GGAGGTTGTG ATGGTAATGG AAACAGATTT AGCTCTGAAA CTGAGTGTCT GCAAACTTGC 
TCTTCCAAAA GTAAAAAAGC TAATGGGGAA GTGCGGAGCC TTAAGAAAGA TGTCTGTACA 
ATGAAACAAG AGGAAGGAAA ATGTGCAGAT TACATCCTCA AGTGGTCCTA CAACCAAGGC 
AAAAAAGCTT GTGTGCAGTT CTGGTACGGT GGCTGTGGAG GAAATGGCAA TCGTTTTGAC 
ACACAGGAAC AGTGTGAAGC CACCTGCATG TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820988.2
CDS	131..8446
Translation	MGFWKVFLACVLGSYSVNVSASQKTVCTEASIADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREPAIAVKNAGIILYAIGIKDAVLSELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEPAKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRRHAEELKSVGVTTISVGIGNSDRKELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKPAAKLRRSGVEVFAVGFQNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELCTPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNPAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNPQESCKKTEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVPQELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVNEDAAALRSSGVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQVCEPSDQPIIGKECSLDITISVDYSRRAMLSTVPRVQQKLQQFLPPVLQRISSLSNISCASGSQINLRFRYAVAAQDRLTFDSDFEKFNEDIIKKFIAEQSSVDTYLNAKFLQSLWNKYIGLPSQKAKIILVFTDGLDESSEVLKATTESLRKQGLDALLFIALEGVKDFTELQNLEFGRGFSYQQPLSIALHDLPSVMLKEMDTVAERKCCNVVCKCLGQDGPLGSPGALGTKGQPGIKGSQGHPGEEGGHGERGFRGLNGTRGESGCPGRRGLKGARGIRGDKGDDGEFGIDGVPGEQGEPGGRGASGERGDTGAQGRKGPRGQPGEKGENGLRGDPGEPGTNINIQGPIGLKGNPGRQGEAGSDGVEGEPGNNGPNGPQGRRGPPGLKGARGFPGEPGNKGDSGIQGIQGSQGMPGPPGPQGPQGLSGRQGDAGAPGASGSFGKPGAPGLKGEPGENGEKGEPGIPGRRGLPGLDGPDGYGPPGLKGEKGHLGFPGDPGPQGEDGDPGIAGNGGPKGISGRRGNSGPPGTNGQRGERGPRGPMGAKGLPGTMPMTPCELVAFTRENCPCFASASKCPVYPTEIVFALDASQDVPPAAFQRMKNTIISIIEKLDISESNCPRGARVAVVAYNSNTKYLIRFSDYKRKSLLLEAIRGISPERSSSRRNIGEAMKFVGRNIFKRIRHGIHVRKVAVFFANGPSPDADAINSAVLEYSALDIIPIVIAFNNVPNVVAAFSADETRRFQVFVWSRPEDQRLELISYCTLCYDKCTPDEECELDQMPATLIDMDIAFVLDSSHNVQADVFEEMKDFVSSMLDTVYISTRPAVSETEARVALVQQAVKDFGPNRNTSPVKTEFDLLRYNSANMMKRHIKQSVRQLNGPSAVGNSVQWVIDNIFLKTPMPRKHKVLFLILASPTSYWDREKLKEVSKKAKCQGFAIFVLGFGKTISELEMTQLPSLPQDQHLLRLISMSKPELVYAKKFSHAFFNLLDRGFNHYPPAVLQAECQGRGDSSFSVVRSFPDTRLTQVDTAAAEVPDETIEVVEVLGTPAPEEIVGKKTEKIIINKVADNCLLDVNHGTACRGYQRMWYYIKSIDACSQFWYGGCDGNGNRFSSETECLQTCSSKSKKANGEVRSLKKDVCTMKQEEGKCADYILKWSYNQGKKACVQFWYGGCGGNGNRFDTQEQCEATCMS

Target ORF information:

RefSeq Version	XM_012965534.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen type VI alpha 5 chain (col6a5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965534.2

ORF Insert Sequence:

ATGGGATTTT GGAAAGTATT TCTCGCCTGC GTGCTTGGCA GCTACTCAGT CAATGTCTCA 
GCTTCTCAGA AAACAGTGTG CACAGAGGCC AGTATTGCAG ATATTGTATT CCTTATGGAT 
GGATCCTGGA GCATCGGAAC GGAAAACTTT ATCACAATGA AGAACTTTTT GTACACTTTG 
GTTAATGGTT TTGATGTTGG CCTAGATAAG ATCCAGATTG GCCTAATCCA GTACAGTGAT 
AATGCACGTA CAGAATTCTT CCTAAACAGT TACAGTAACA AAGAAGATGT TTTGAAATAT 
ATTCAGAATC TAAAGTATAA AGGGGGTGGG ACAAAGACTG GCTTAAGTCT GGAGTTCATG 
CTGACGCAGC ACTTTAGTGA AGCTGCAGGC AGCAGAGCAG CAGAAGGTGT GCCTCAGATT 
GCAGTCGTTA TAACTGATGG ACAAGCTCAA GATAGCATCA GGGAGCCTGC CATTGCAGTG 
AAGAACGCTG GAATCATACT GTATGCCATT GGCATTAAAG ATGCAGTTTT ATCTGAGCTT 
AATGAAATAG CCAGTGATCC AGATGACAAA CATGTTTATA GCGTTGCAGA CTTTAATGCA 
CTGCAAAGTA TTTCCCAAAA CATGATCCAG GTGCTCTGCA CAACTGTAGA AGAAGCAGCA 
AGACAGACAG GTCAAATTGC TCAAGTTTGC AGAACTGCCA ATCAAGCTGA CATTGTGCTG 
CTAGTCGAAA GCACAACAAG AATGGGTGAC GCTACTTTTG AAAAAGCAAA GAATTTTCTT 
TATGATTTAG TTTCTAATTT GGATGTTGGA ATTAATAAGA TTCGCATTGG GCTTGTAACC 
TACAATGATG AGACAAATCC AGAATTTTTG CTAAACAGCT ATTCTTCGAA AACTGAAATC 
TTAGAAAGTA TTCAGAATAT GAAATATGTA GAAGGATATA CCTACACAGG GAGAGCCCTT 
GAGTATGTAA ATACAACCTA CTTTACACAG GCAGCAGGCA GTAGATTTGA AGAGAGCGTC 
GCTCAAATTC TTATCATTGT GACAGAAGGA GACTCTTCAG ATACCCTCAC TGAGCCTGCT 
AAAGAACTCA AATCAAGAGG CATATCTGTT TATGTGGTTG GGACAAACAT CAAGTACGAT 
AGACAGCTTC AGGAAGCATC AAGCAAGCCC GACGAAAAGT TCTTTTATCA ACTCGATGAT 
TTTGATGACT CTGAGAATGT CACTGAGCAG CTGTTAAAAA ACCTGTGCTT TAGCATAGAT 
TTGAATATTC AAGTATATTC AAAGCGCTAC GCTGATGTTG TATTTCTAGT CGATTCTTCT 
ACAAGCATGG GAACCATCTT TTTTCAAAAG ATGAAGGATT TCATCATACA CATAATAAAC 
CAGTTAAATG TTGGAATAAA TAAACACCGT ATAGGACTGG CACAGTACAG TGGTCTACCC 
CAAACTGAAT TTCTACTGAA TCATTATGAA ACCAAAGAGG AGATACTAAA GCACATTAAG 
GAAACATTCA CATACAGAGG TGGCCCCTTA AAAACAGGAC ATGCTTTGGA ATTTGTACGC 
TCAACATTTT TCATAGAAGA GGCTGGGAGT AGGATTAACT ACGGCAACCC CCAGTTCTTA 
GTGGTCATAA CATCATCAAA ATCAGAGGAT GCTGTTAGAA GACATGCCGA GGAACTCAAA 
AGTGTTGGGG TCACAACCAT CTCTGTTGGC ATTGGCAATT CTGATCGAAA AGAACTGGAA 
AAAATTGCTA CTGATCCTTT TGTTTTCCAG ACCACAGGTT TACAGCACAT TTCAAATTTA 
CAGCAGGATG TGGCCAATGT TATTATAGCA GAAGACATGC TACAGTTTTC CCCAGTATCA 
GTTGTACCAG CAGTTTGCTC CAGTGCTTCT GTAGCTGACA TTGTGTTTCT AATTGATGAA 
TCTAGCAGCA TTGGCCCCAT CAACTTTCAA CTAACCAGAG TCTTTCTACA CAAGGTTGTT 
AGTGCACTGG ATATTTCCTT GAGTAATGTT AGAGTGGGCT TAGTGCTTTA CAGTGATGAG 
CCAAGACTTG AACTTAAACT GAACACTTTT AATGAAAAAT ATGAAATTTT GGACTTCATA 
ACTAAGCTGC CATACAGAGG AGGCAAAGCA CATACAGGTG CTGCGCTGGA CTTTCTAAGG 
AAAAAAATGT TCACCAAACA AAATGGCGGC CGGCCACATC AAGGAGTTCA GCAGATAGCG 
GTGGTTATGA CTAATGGGCA GTCCATGGAT AATTTTACTA AACCTGCTGC AAAACTGAGA 
CGGTCTGGCG TGGAGGTTTT TGCTGTTGGC TTTCAGAATA TCAATGATAC AGAACTGGAT 
ATCATAGCAT CTCACCCACC GCGAAAACAT GTCACAAATG TGGAATCTTT CCTGCAGCTA 
TCCAACCTTG AGTTCAGAAT ACAGAAACGA CTTTGCAATG AAATTGTTGT GAAGTCATTT 
GCAGTACCTG CAATTGCTCG TGCAGTGAAG GAAGGGTGTG TGGATACAGA AGAAGCAGAT 
ATATACTTTC TAATTGATGG TTCTGGCAGT ATATATCCAG AGGACTTTGA AGATATGAAA 
AAATTCATGA TTGAATTAAT AAGCATGTTT CAAGTTGGTG CCAACAGAGT ACGCTTTGGA 
GTTGTCCAAT ACTCAGATGT TCGTAGAACT GAATTTTTCA TCAGTGAACA CAACACCCAG 
AAAATGTTAA AGGATGCTAT TTCTCAAATA GAACAGTTGG GCGGTGGTAC CCTAACAGGA 
GAAGCTTTAA CGTCAATGAA GCAACTTTTT GTTAATGCAG CTAAAGATCG CCCTCATAAA 
GTACCCCAGT CATTGGTTGT CATTACAGAT GGAGAATCTC AAGACCGGGT CACAGAAGCA 
GCTGCAGAAA TTAGAAATGA TGGCATTACT ATATTTGCAA TTGGTGTTAA AAATGCAGTG 
GAAGAAGAGA TTAGAGATAT AGCAGGGTCA AATGAGAAGA TGTTTTTTGT TAACAACTTT 
GATTCCCTGA AGGTGATCAA GAATGATTTG GCTCGTGAAT TGTGTACACC TGAGGCATGT 
AAGAACATGA AAGCTGATAT CGTGTTTCTG GTTGATAGCT CTGCAAGCAT TAACAGTGAT 
GATTATGAAA CAATGAAAGA ATTCATGGAG TCAATGGTCA AGCAGGCAGA AATCGGCCCT 
GATAGAGTCC AGATTGGTTT AATTCAGTTT AGCTCTGAAA CAAAGGAAGA ATTTCCTCTC 
AACAGGTACA AAAGAAAGGA TGAAATACAA TCAGCAATAA GAGGCATCCA ACAACTGAGC 
CAGGGAACCC TTATGGGGGA AGCCCTTAAA TATACATTAC CTTATTTTAG TGCTTCCAAA 
GGAGGCCGTG TAAATACCAA ACAATATCTG ATCGTCATAA CAGATGGTGA GGCCCAGGAT 
GCTGTGGGAA ACCCAGCCAA AGCAATAAGG GACCATGGAG TTATCATTTA TGCCATAGGA 
GTACAACAAG CCAACAACAC ACAACTTCTG GAGATTGCTG GCAAACAAGA GCAGGTGTAT 
TATGAAGATA GCTTTGATTC CCTAGCTTTT TTGAACAAGA ACATCATGTT TGAGATTTGC 
AATCCTCAAG AATCTTGCAA AAAGACTGAG GTGGCAGACA TCATATTTCT GTTGGATGCT 
TCTGCAAGCA TAACCAGAGG AGAGTTTAGA CTCATGCAGA GATTTGTGGA GGCTGTTGTC 
AATGACTCTT TGGTTGGAAA GGATAATGTT CAATTTGGAG CAGTTGTTTA CGGCACAAAT 
CCAGCAGAAC AGTTTTCACT CAATACGTAT TCAACTAAAC TTGATATTCT TAAGGCTGTA 
TTTAGTTTAC CCCAAGTATC TGGATACACA TACACTGCAA AAGCGCTGGA ATACACTCGG 
ATACGGTTTG GTACATCATA TGGTGGAAGA CCAGGAATTT CACATATTCT AATTCTAGTC 
ACAGATGGAG CCACTACTGA AGCTGATCGC CCTAACCTAC CAATAGTATC AAAGGCTTTG 
AAGGATGATG GCATCATTGT CTTTGCAGTA GGAGTTGGCA AGGCAGTTCC TCAAGAGCTT 
CAGCAGATTG CAGGATACCC AGACAGATGG TTCTTAGTCC AGAACTACAA AGGCCTGGAC 
AATATACATG ATAATATAAC ACAGGTTGTA TGTGATGAAT CAAAACCAGC TTGCTCACAT 
GAACAGCTTG ATCTTGTTTT CCTGATTGAC GGCTCAGCAA GTATCACTTC TAGCAACTTC 
ACAAGTGCCA AGACTTTCAT GAAAGAAATC GTTGACAGCT TTACAATTTC AGAGAACAGA 
GTGCGTATTG GAGTCGCACA GTACAGTGCA AATCCCAAGA AAGAATTCTT CTTAAATGAG 
TACTACTCCA GCTCCGACAT GAAGAAACAG ATTGATAGCA TCTCCCAACT GAAAGCTACC 
ACTTACACTG GCAAAGGCCT ACGATTTGTC AAACAGTTCT TTGATCCAGC AAATGGCGGC 
CGTAAGAATG TTCCGCAGTA CCTGATAGTC ATGACTGATG GCATGTCTAA TGACAGCGTA 
AATGAAGATG CTGCTGCTTT GCGAAGCAGT GGAGTAAAGA TCTTTTCCAT TGGTATTGGT 
CTGCGGAACA GTTTTGAGCT TGTTATGATT GCCGGGTCAC CCAAAAATGT TTACGAAGTG 
GAAACGTTCC AAGCGCTGGA CTCTATAAAG AGGCAGATTG TGGCACAAGT CTGTGAGCCA 
TCTGATCAGC CTATTATAGG TAAAGAATGC AGCTTAGATA TCACCATATC CGTTGACTAC 
TCAAGAAGAG CAATGCTATC TACTGTCCCC CGTGTGCAGC AGAAATTGCA GCAGTTCCTC 
CCTCCGGTGC TTCAGCGCAT TTCCTCGCTT AGCAATATCA GCTGTGCCTC TGGATCTCAA 
ATTAACTTAC GCTTCAGATA CGCTGTTGCA GCACAGGACA GACTGACATT TGATTCCGAC 
TTTGAGAAAT TTAACGAGGA CATCATTAAG AAGTTTATAG CTGAACAGTC TTCTGTTGAT 
ACATATTTAA ATGCAAAGTT TCTTCAGTCC CTCTGGAACA AATACATAGG TTTGCCGTCT 
CAGAAAGCAA AAATTATTCT GGTTTTTACT GATGGACTGG ATGAAAGTTC TGAAGTACTC 
AAGGCGACTA CTGAATCTCT TCGGAAACAA GGGTTGGATG CACTGTTATT CATTGCTCTT 
GAGGGGGTGA AGGATTTTAC AGAGCTGCAA AACTTAGAGT TTGGAAGAGG ATTTTCTTAC 
CAGCAACCTC TGAGCATTGC GCTGCATGAT CTGCCAAGTG TAATGCTAAA AGAAATGGAT 
ACCGTTGCAG AAAGGAAATG CTGTAATGTA GTTTGTAAAT GTTTAGGACA AGATGGACCT 
CTAGGCTCAC CAGGGGCTTT AGGAACAAAG GGTCAGCCTG GTATAAAGGG ATCTCAAGGT 
CATCCTGGAG AGGAAGGCGG TCATGGTGAA AGAGGATTCC GTGGGCTGAA TGGGACAAGA 
GGCGAAAGTG GATGTCCTGG TCGTAGGGGA TTAAAGGGTG CCAGAGGTAT TCGTGGTGAC 
AAGGGTGATG ATGGAGAGTT TGGAATTGAT GGTGTGCCTG GAGAGCAGGG TGAACCTGGA 
GGGCGTGGAG CATCAGGAGA ACGTGGAGAC ACTGGAGCTC AGGGCAGAAA AGGTCCTCGT 
GGGCAACCTG GAGAAAAAGG GGAAAATGGA CTGAGGGGAG ACCCAGGTGA GCCTGGAACT 
AACATTAACA TTCAGGGACC TATAGGACTG AAAGGAAACC CAGGACGTCA GGGAGAGGCG 
GGAAGTGACG GTGTGGAAGG AGAGCCAGGA AATAATGGAC CTAATGGTCC ACAAGGCCGA 
AGAGGGCCTC CTGGTTTAAA GGGAGCACGA GGATTTCCTG GAGAGCCAGG GAACAAAGGT 
GATAGTGGAA TCCAAGGTAT ACAGGGTTCC CAAGGCATGC CTGGACCACC AGGTCCACAG 
GGACCACAAG GGTTATCTGG ACGTCAGGGA GATGCAGGTG CCCCTGGTGC ATCAGGTTCC 
TTTGGCAAAC CCGGAGCTCC AGGACTAAAG GGGGAACCTG GAGAAAACGG AGAGAAGGGT 
GAGCCAGGCA TTCCGGGTCG CAGAGGTTTG CCAGGGCTGG ATGGTCCTGA TGGATATGGT 
CCCCCAGGCT TAAAGGGAGA GAAGGGTCAT TTAGGGTTTC CTGGGGATCC TGGGCCTCAG 
GGAGAAGATG GTGATCCTGG AATAGCTGGA AATGGTGGCC CAAAAGGAAT TAGTGGAAGA 
AGGGGTAATT CTGGCCCTCC AGGAACTAAT GGACAAAGGG GTGAGAGGGG ACCACGTGGA 
CCTATGGGCG CCAAAGGATT GCCAGGAACT ATGCCTATGA CGCCTTGTGA ACTTGTGGCG 
TTTACTCGAG AGAATTGTCC TTGCTTCGCA AGTGCTTCAA AATGTCCAGT TTACCCAACT 
GAAATAGTGT TTGCTCTGGA TGCATCTCAG GACGTTCCCC CGGCTGCTTT CCAAAGGATG 
AAGAACACCA TTATTTCCAT AATAGAAAAG CTGGACATTA GTGAGAGCAA TTGCCCTAGA 
GGAGCCCGTG TTGCTGTCGT TGCGTATAAC AGTAATACCA AATACCTCAT TCGTTTCTCT 
GACTACAAAC GGAAAAGCCT ACTGCTGGAA GCAATTCGTG GCATCTCTCC AGAAAGATCA 
TCTTCAAGGA GAAATATTGG AGAAGCCATG AAGTTTGTAG GGAGGAACAT ATTCAAACGC 
ATTCGTCATG GAATCCATGT TAGAAAAGTG GCTGTGTTCT TTGCTAATGG CCCATCCCCA 
GATGCAGATG CAATAAACTC GGCAGTGCTG GAATACAGTG CTCTTGACAT TATTCCTATT 
GTGATTGCAT TTAATAATGT TCCCAATGTC GTAGCAGCTT TTTCGGCCGA CGAAACTAGA 
AGATTCCAAG TGTTTGTATG GAGTAGGCCT GAGGATCAGA GACTAGAACT TATTTCTTAT 
TGTACTCTTT GTTATGATAA GTGTACACCT GATGAAGAAT GTGAGCTGGA TCAAATGCCT 
GCAACCCTAA TAGATATGGA CATAGCTTTT GTATTAGACA GTTCACACAA TGTGCAAGCT 
GATGTCTTTG AGGAAATGAA AGACTTTGTG AGCAGTATGC TGGACACCGT CTACATATCA 
ACGCGGCCCG CAGTTTCTGA AACAGAAGCC AGAGTTGCCT TAGTACAACA GGCAGTTAAG 
GACTTTGGTC CAAATAGGAA TACTAGCCCA GTTAAAACTG AGTTCGACCT TCTTCGCTAC 
AACAGCGCAA ATATGATGAA GAGGCACATC AAACAATCTG TCAGGCAACT GAATGGTCCT 
TCTGCAGTTG GCAATTCTGT TCAGTGGGTC ATTGATAATA TCTTCTTAAA AACACCAATG 
CCAAGAAAGC ATAAAGTACT TTTCCTCATT CTTGCCAGCC CAACAAGCTA TTGGGACAGA 
GAAAAATTGA AAGAAGTCTC CAAAAAAGCT AAGTGTCAAG GATTTGCAAT ATTTGTACTG 
GGTTTTGGAA AAACGATAAG TGAGCTGGAA ATGACACAGC TGCCGAGCTT GCCACAAGAC 
CAACATTTGC TTCGCTTGAT AAGCATGTCA AAACCAGAAC TTGTCTATGC AAAGAAGTTC 
AGTCATGCCT TCTTCAATCT ACTCGACCGT GGCTTTAATC ACTACCCACC CGCAGTATTG 
CAAGCAGAGT GCCAAGGGCG TGGGGACTCA TCTTTTTCAG TTGTAAGAAG CTTCCCTGAC 
ACACGGTTGA CACAAGTTGA CACTGCTGCA GCAGAAGTTC CAGATGAGAC TATAGAAGTA 
GTGGAGGTCC TTGGTACACC TGCACCTGAA GAAATTGTGG GGAAAAAAAC AGAGAAAATA 
ATCATAAACA AAGTTGCAGA CAACTGCTTA TTGGATGTGA ACCATGGCAC AGCGTGCAGG 
GGCTATCAGC GCATGTGGTA TTATATAAAA TCTATTGATG CATGCTCCCA GTTCTGGTAT 
GGAGGTTGTG ATGGTAATGG AAACAGATTT AGCTCTGAAA CTGAGTGTCT GCAAACTTGC 
TCTTCCAAAA GTAAAAAAGC TAATGGGGAA GTGCGGAGCC TTAAGAAAGA TGTCTGTACA 
ATGAAACAAG AGGAAGGAAA ATGTGCAGAT TACATCCTCA AGTGGTCCTA CAACCAAGGC 
AAAAAAGCTT GTGTGCAGTT CTGGTACGGT GGCTGTGGAG GAAATGGCAA TCGTTTTGAC 
ACACAGGAAC AGTGTGAAGC CACCTGCATG TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col6a5 ( XM_012965535.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820989.2 Xenopus tropicalis collagen type VI alpha 5 chain (col6a5), transcript variant X3, mRNA.

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CloneID	OXa59875
Clone ID Related Accession (Same CDS sequence)	XM_012965535.2
Accession Version	XM_012965535.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-6(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965535.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTTT GGAAAGTATT TCTCGCCTGC GTGCTTGGCA GCTACTCAGT CAATGTCTCA 
GCTTCTCAGA AAACAGTGTG CACAGAGGCC AGTATTGCAG ATATTGTATT CCTTATGGAT 
GGATCCTGGA GCATCGGAAC GGAAAACTTT ATCACAATGA AGAACTTTTT GTACACTTTG 
GTTAATGGTT TTGATGTTGG CCTAGATAAG ATCCAGATTG GCCTAATCCA GTACAGTGAT 
AATGCACGTA CAGAATTCTT CCTAAACAGT TACAGTAACA AAGAAGATGT TTTGAAATAT 
ATTCAGAATC TAAAGTATAA AGGGGGTGGG ACAAAGACTG GCTTAAGTCT GGAGTTCATG 
CTGACGCAGC ACTTTAGTGA AGCTGCAGGC AGCAGAGCAG CAGAAGGTGT GCCTCAGATT 
GCAGTCGTTA TAACTGATGG ACAAGCTCAA GATAGCATCA GGGAGCCTGC CATTGCAGTG 
AAGAACGCTG GAATCATACT GTATGCCATT GGCATTAAAG ATGCAGTTTT ATCTGAGCTT 
AATGAAATAG CCAGTGATCC AGATGACAAA CATGTTTATA GCGTTGCAGA CTTTAATGCA 
CTGCAAAGTA TTTCCCAAAA CATGATCCAG GTGCTCTGCA CAACTGTAGA AGAAGCAGCA 
AGACAGACAG GTCAAATTGC TCAAGTTTGC AGAACTGCCA ATCAAGCTGA CATTGTGCTG 
CTAGTCGAAA GCACAACAAG AATGGGTGAC GCTACTTTTG AAAAAGCAAA GAATTTTCTT 
TATGATTTAG TTTCTAATTT GGATGTTGGA ATTAATAAGA TTCGCATTGG GCTTGTAACC 
TACAATGATG AGACAAATCC AGAATTTTTG CTAAACAGCT ATTCTTCGAA AACTGAAATC 
TTAGAAAGTA TTCAGAATAT GAAATATGTA GAAGGATATA CCTACACAGG GAGAGCCCTT 
GAGTATGTAA ATACAACCTA CTTTACACAG GCAGCAGGCA GTAGATTTGA AGAGAGCGTC 
GCTCAAATTC TTATCATTGT GACAGAAGGA GACTCTTCAG ATACCCTCAC TGAGCCTGCT 
AAAGAACTCA AATCAAGAGG CATATCTGTT TATGTGGTTG GGACAAACAT CAAGTACGAT 
AGACAGCTTC AGGAAGCATC AAGCAAGCCC GACGAAAAGT TCTTTTATCA ACTCGATGAT 
TTTGATGACT CTGAGAATGT CACTGAGCAG CTGTTAAAAA ACCTGTGCTT TAGCATAGAT 
TTGAATATTC AAGTATATTC AAAGCGCTAC GCTGATGTTG TATTTCTAGT CGATTCTTCT 
ACAAGCATGG GAACCATCTT TTTTCAAAAG ATGAAGGATT TCATCATACA CATAATAAAC 
CAGTTAAATG TTGGAATAAA TAAACACCGT ATAGGACTGG CACAGTACAG TGGTCTACCC 
CAAACTGAAT TTCTACTGAA TCATTATGAA ACCAAAGAGG AGATACTAAA GCACATTAAG 
GAAACATTCA CATACAGAGG TGGCCCCTTA AAAACAGGAC ATGCTTTGGA ATTTGTACGC 
TCAACATTTT TCATAGAAGA GGCTGGGAGT AGGATTAACT ACGGCAACCC CCAGTTCTTA 
GTGGTCATAA CATCATCAAA ATCAGAGGAT GCTGTTAGAA GACATGCCGA GGAACTCAAA 
AGTGTTGGGG TCACAACCAT CTCTGTTGGC ATTGGCAATT CTGATCGAAA AGAACTGGAA 
AAAATTGCTA CTGATCCTTT TGTTTTCCAG ACCACAGGTT TACAGCACAT TTCAAATTTA 
CAGCAGGATG TGGCCAATGT TATTATAGCA GAAGACATGC TACAGTTTTC CCCAGTATCA 
GTTGTACCAG CAGTTTGCTC CAGTGCTTCT GTAGCTGACA TTGTGTTTCT AATTGATGAA 
TCTAGCAGCA TTGGCCCCAT CAACTTTCAA CTAACCAGAG TCTTTCTACA CAAGGTTGTT 
AGTGCACTGG ATATTTCCTT GAGTAATGTT AGAGTGGGCT TAGTGCTTTA CAGTGATGAG 
CCAAGACTTG AACTTAAACT GAACACTTTT AATGAAAAAT ATGAAATTTT GGACTTCATA 
ACTAAGCTGC CATACAGAGG AGGCAAAGCA CATACAGGTG CTGCGCTGGA CTTTCTAAGG 
AAAAAAATGT TCACCAAACA AAATGGCGGC CGGCCACATC AAGGAGTTCA GCAGATAGCG 
GTGGTTATGA CTAATGGGCA GTCCATGGAT AATTTTACTA AACCTGCTGC AAAACTGAGA 
CGGTCTGGCG TGGAGGTTTT TGCTGTTGGC TTTCAGAATA TCAATGATAC AGAACTGGAT 
ATCATAGCAT CTCACCCACC GCGAAAACAT GTCACAAATG TGGAATCTTT CCTGCAGCTA 
TCCAACCTTG AGTTCAGAAT ACAGAAACGA CTTTGCAATG AAATTGTTGT GAAGTCATTT 
GCAGTACCTG CAATTGCTCG TGCAGTGAAG GAAGGGTGTG TGGATACAGA AGAAGCAGAT 
ATATACTTTC TAATTGATGG TTCTGGCAGT ATATATCCAG AGGACTTTGA AGATATGAAA 
AAATTCATGA TTGAATTAAT AAGCATGTTT CAAGTTGGTG CCAACAGAGT ACGCTTTGGA 
GTTGTCCAAT ACTCAGATGT TCGTAGAACT GAATTTTTCA TCAGTGAACA CAACACCCAG 
AAAATGTTAA AGGATGCTAT TTCTCAAATA GAACAGTTGG GCGGTGGTAC CCTAACAGGA 
GAAGCTTTAA CGTCAATGAA GCAACTTTTT GTTAATGCAG CTAAAGATCG CCCTCATAAA 
GTACCCCAGT CATTGGTTGT CATTACAGAT GGAGAATCTC AAGACCGGGT CACAGAAGCA 
GCTGCAGAAA TTAGAAATGA TGGCATTACT ATATTTGCAA TTGGTGTTAA AAATGCAGTG 
GAAGAAGAGA TTAGAGATAT AGCAGGGTCA AATGAGAAGA TGTTTTTTGT TAACAACTTT 
GATTCCCTGA AGGTGATCAA GAATGATTTG GCTCGTGAAT TGTGTACACC TGAGGCATGT 
AAGAACATGA AAGCTGATAT CGTGTTTCTG GTTGATAGCT CTGCAAGCAT TAACAGTGAT 
GATTATGAAA CAATGAAAGA ATTCATGGAG TCAATGGTCA AGCAGGCAGA AATCGGCCCT 
GATAGAGTCC AGATTGGTTT AATTCAGTTT AGCTCTGAAA CAAAGGAAGA ATTTCCTCTC 
AACAGGTACA AAAGAAAGGA TGAAATACAA TCAGCAATAA GAGGCATCCA ACAACTGAGC 
CAGGGAACCC TTATGGGGGA AGCCCTTAAA TATACATTAC CTTATTTTAG TGCTTCCAAA 
GGAGGCCGTG TAAATACCAA ACAATATCTG ATCGTCATAA CAGATGGTGA GGCCCAGGAT 
GCTGTGGGAA ACCCAGCCAA AGCAATAAGG GACCATGGAG TTATCATTTA TGCCATAGGA 
GTACAACAAG CCAACAACAC ACAACTTCTG GAGATTGCTG GCAAACAAGA GCAGGTGTAT 
TATGAAGATA GCTTTGATTC CCTAGCTTTT TTGAACAAGA ACATCATGTT TGAGATTTGC 
AATCCTCAAG AATCTTGCAA AAAGACTGAG GTGGCAGACA TCATATTTCT GTTGGATGCT 
TCTGCAAGCA TAACCAGAGG AGAGTTTAGA CTCATGCAGA GATTTGTGGA GGCTGTTGTC 
AATGACTCTT TGGTTGGAAA GGATAATGTT CAATTTGGAG CAGTTGTTTA CGGCACAAAT 
CCAGCAGAAC AGTTTTCACT CAATACGTAT TCAACTAAAC TTGATATTCT TAAGGCTGTA 
TTTAGTTTAC CCCAAGTATC TGGATACACA TACACTGCAA AAGCGCTGGA ATACACTCGG 
ATACGGTTTG GTACATCATA TGGTGGAAGA CCAGGAATTT CACATATTCT AATTCTAGTC 
ACAGATGGAG CCACTACTGA AGCTGATCGC CCTAACCTAC CAATAGTATC AAAGGCTTTG 
AAGGATGATG GCATCATTGT CTTTGCAGTA GGAGTTGGCA AGGCAGTTCC TCAAGAGCTT 
CAGCAGATTG CAGGATACCC AGACAGATGG TTCTTAGTCC AGAACTACAA AGGCCTGGAC 
AATATACATG ATAATATAAC ACAGGTTGTA TGTGATGAAT CAAAACCAGC TTGCTCACAT 
GAACAGCTTG ATCTTGTTTT CCTGATTGAC GGCTCAGCAA GTATCACTTC TAGCAACTTC 
ACAAGTGCCA AGACTTTCAT GAAAGAAATC GTTGACAGCT TTACAATTTC AGAGAACAGA 
GTGCGTATTG GAGTCGCACA GTACAGTGCA AATCCCAAGA AAGAATTCTT CTTAAATGAG 
TACTACTCCA GCTCCGACAT GAAGAAACAG ATTGATAGCA TCTCCCAACT GAAAGCTACC 
ACTTACACTG GCAAAGGCCT ACGATTTGTC AAACAGTTCT TTGATCCAGC AAATGGCGGC 
CGTAAGAATG TTCCGCAGTA CCTGATAGTC ATGACTGATG GCATGTCTAA TGACAGCGTA 
AATGAAGATG CTGCTGCTTT GCGAAGCAGT GGAGTAAAGA TCTTTTCCAT TGGTATTGGT 
CTGCGGAACA GTTTTGAGCT TGTTATGATT GCCGGGTCAC CCAAAAATGT TTACGAAGTG 
GAAACGTTCC AAGCGCTGGA CTCTATAAAG AGGCAGATTG TGGCACAAGT CTGTGAGCCA 
TCTGATCAGC CTATTATAGG TAAAGAATGC AGCTTAGATA TCACCATATC CGTTGACTAC 
TCAAGAAGAG CAATGCTATC TACTGTCCCC CGTGTGCAGC AGAAATTGCA GCAGTTCCTC 
CCTCCGGTGC TTCAGCGCAT TTCCTCGCTT AGCAATATCA GCTGTGCCTC TGGATCTCAA 
ATTAACTTAC GCTTCAGATA CGCTGTTGCA GCACAGGACA GACTGACATT TGATTCCGAC 
TTTGAGAAAT TTAACGAGGA CATCATTAAG AAGTTTATAG CTGAACAGTC TTCTGTTGAT 
ACATATTTAA ATGCAAAGTT TCTTCAGTCC CTCTGGAACA AATACATAGG TTTGCCGTCT 
CAGAAAGCAA AAATTATTCT GGTTTTTACT GATGGACTGG ATGAAAGTTC TGAAGTACTC 
AAGGCGACTA CTGAATCTCT TCGGAAACAA GGGTTGGATG CACTGTTATT CATTGCTCTT 
GAGGGGGTGA AGGATTTTAC AGAGCTGCAA AACTTAGAGT TTGGAAGAGG ATTTTCTTAC 
CAGCAACCTC TGAGCATTGC GCTGCATGAT CTGCCAAGTG TAATGCTAAA AGAAATGGAT 
ACCGTTGCAG AAAGGAAATG CTGTAATGTA GTTTGTAAAT GTTTAGGACA AGATGGACCT 
CTAGGCTCAC CAGGGGCTTT AGGAACAAAG GGTCAGCCTG GTATAAAGGG ATCTCAAGGT 
CATCCTGGAG AGGAAGGCGG TCATGGTGAA AGAGGATTCC GTGGGCTGAA TGGGACAAGA 
GGCGAAAGTG GATGTCCTGG TCGTAGGGGA TTAAAGGGTG CCAGAGGTAT TCGTGGTGAC 
AAGGGTGATG ATGGAGAGTT TGGAATTGAT GGTGTGCCTG GAGAGCAGGG TGAACCTGGA 
GGGCGTGGAG CATCAGGAGA ACGTGGAGAC ACTGGAGCTC AGGGCAGAAA AGGTCCTCGT 
GGGCAACCTG GAGAAAAAGG GGAAAATGGA CTGAGGGGAG ACCCAGGTGA GCCTGGAACT 
AACATTAACA TTCAGGGACC TATAGGACTG AAAGGAAACC CAGGACGTCA GGGAGAGGCG 
GGAAGTGACG GTGTGGAAGG AGAGCCAGGA AATAATGGAC CTAATGGTCC ACAAGGCCGA 
AGAGGGCCTC CTGGTTTAAA GGGAGCACGA GGATTTCCTG GAGAGCCAGG GAACAAAGGT 
GATAGTGGAA TCCAAGGTAT ACAGGGTTCC CAAGGCATGC CTGGACCACC AGGTCCACAG 
GGACCACAAG GGTTATCTGG ACGTCAGGGA GATGCAGGTG CCCCTGGTGC ATCAGGTTCC 
TTTGGCAAAC CCGGAGCTCC AGGACTAAAG GGGGAACCTG GAGAAAACGG AGAGAAGGGT 
GAGCCAGGCA TTCCGGGTCG CAGAGGTTTG CCAGGGCTGG ATGGTCCTGA TGGATATGGT 
CCCCCAGGCT TAAAGGGAGA GAAGGGTCAT TTAGGGTTTC CTGGGGATCC TGGGCCTCAG 
GGAGAAGATG GTGATCCTGG AATAGCTGGA AATGGTGGCC CAAAAGGAAT TAGTGGAAGA 
AGGGGTAATT CTGGCCCTCC AGGAACTAAT GGACAAAGGG GTGAGAGGGG ACCACGTGGA 
CCTATGGGCG CCAAAGGATT GCCAGGAACT ATGCCTATGA CGCCTTGTGA ACTTGTGGCG 
TTTACTCGAG AGAATTGTCC TTGCTTCGCA AGTGCTTCAA AATGTCCAGT TTACCCAACT 
GAAATAGTGT TTGCTCTGGA TGCATCTCAG GACGTTCCCC CGGCTGCTTT CCAAAGGATG 
AAGAACACCA TTATTTCCAT AATAGAAAAG CTGGACATTA GTGAGAGCAA TTGCCCTAGA 
GGAGCCCGTG TTGCTGTCGT TGCGTATAAC AGTAATACCA AATACCTCAT TCGTTTCTCT 
GACTACAAAC GGAAAAGCCT ACTGCTGGAA GCAATTCGTG GCATCTCTCC AGAAAGATCA 
TCTTCAAGGA GAAATATTGG AGAAGCCATG AAGTTTGTAG GGAGGAACAT ATTCAAACGC 
ATTCGTCATG GAATCCATGT TAGAAAAGTG GCTGTGTTCT TTGCTAATGG CCCATCCCCA 
GATGCAGATG CAATAAACTC GGCAGTGCTG GAATACAGTG CTCTTGACAT TATTCCTATT 
GTGATTGCAT TTAATAATGT TCCCAATGTC GTAGCAGCTT TTTCGGCCGA CGAAACTAGA 
AGATTCCAAG TGTTTGTATG GAGTAGGCCT GAGGATCAGA GACTAGAACT TATTTCTTAT 
TGTACTCTTT GTTATGATAA GTGTACACCT GATGAAGAAT GTGAGCTGGA TCAAATGCCT 
GCAACCCTAA TAGATATGGA CATAGCTTTT GTATTAGACA GTTCACACAA TGTGCAAGCT 
GATGTCTTTG AGGAAATGAA AGACTTTGTG AGCAGTATGC TGGACACCGT CTACATATCA 
ACGCGGCCCG CAGTTTCTGA AACAGAAGCC AGAGTTGCCT TAGTACAACA GGCAGTTAAG 
GACTTTGGTC CAAATAGGAA TACTAGCCCA GTTAAAACTG AGTTCGACCT TCTTCGCTAC 
AACAGCGCAA ATATGATGAA GAGGCACATC AAACAATCTG TCAGGCAACT GAATGGTCCT 
TCTGCAGTTG GCAATTCTGT TCAGTGGGTC ATTGATAATA TCTTCTTAAA AACACCAATG 
CCAAGAAAGC ATAAAGTACT TTTCCTCATT CTTGCCAGCC CAACAAGCTA TTGGGACAGA 
GAAAAATTGA AAGAAGTCTC CAAAAAAGCT AAGTGTCAAG GATTTGCAAT ATTTGTACTG 
GGTTTTGGAA AAACGATAAG TGAGCTGGAA ATGACACAGC TGCCGAGCTT GCCACAAGAC 
CAACATTTGC TTCGCTTGAT AAGCATGTCA AAACCAGAAC TTGTCTATGC AAAGAAGTTC 
AGTCATGCCT TCTTCAATCT ACTCGACCGT GGCTTTAATC ACTACCCACC CGCAGTATTG 
CAAGCAGAGT GCCAAGGGCG TGGGGACTCA TCTTTTTCAG TTGTAAGAAG CTTCCCTGAC 
ACACGGTTGA CACAAGTTGA CACTGCTGCA GCAGAAGTTC CAGATGAGAC TATAGAAGTA 
GTGGAGGTCC TTGGTACACC TGCACCTGAA GAAATTGTGG GGAAAAAAAC AGAGAAAATA 
ATCATAAACA AAGGTAAAAA AGCTAATGGG GAAGTGCGGA GCCTTAAGAA AGATGTCTGT 
ACAATGAAAC AAGAGGAAGG AAAATGTGCA GATTACATCC TCAAGTGGTC CTACAACCAA 
GGCAAAAAAG CTTGTGTGCA GTTCTGGTAC GGTGGCTGTG GAGGAAATGG CAATCGTTTT 
GACACACAGG AACAGTGTGA AGCCACCTGC ATGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820989.2
CDS	131..8269
Translation	MGFWKVFLACVLGSYSVNVSASQKTVCTEASIADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREPAIAVKNAGIILYAIGIKDAVLSELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEPAKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRRHAEELKSVGVTTISVGIGNSDRKELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKPAAKLRRSGVEVFAVGFQNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELCTPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNPAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNPQESCKKTEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVPQELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVNEDAAALRSSGVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQVCEPSDQPIIGKECSLDITISVDYSRRAMLSTVPRVQQKLQQFLPPVLQRISSLSNISCASGSQINLRFRYAVAAQDRLTFDSDFEKFNEDIIKKFIAEQSSVDTYLNAKFLQSLWNKYIGLPSQKAKIILVFTDGLDESSEVLKATTESLRKQGLDALLFIALEGVKDFTELQNLEFGRGFSYQQPLSIALHDLPSVMLKEMDTVAERKCCNVVCKCLGQDGPLGSPGALGTKGQPGIKGSQGHPGEEGGHGERGFRGLNGTRGESGCPGRRGLKGARGIRGDKGDDGEFGIDGVPGEQGEPGGRGASGERGDTGAQGRKGPRGQPGEKGENGLRGDPGEPGTNINIQGPIGLKGNPGRQGEAGSDGVEGEPGNNGPNGPQGRRGPPGLKGARGFPGEPGNKGDSGIQGIQGSQGMPGPPGPQGPQGLSGRQGDAGAPGASGSFGKPGAPGLKGEPGENGEKGEPGIPGRRGLPGLDGPDGYGPPGLKGEKGHLGFPGDPGPQGEDGDPGIAGNGGPKGISGRRGNSGPPGTNGQRGERGPRGPMGAKGLPGTMPMTPCELVAFTRENCPCFASASKCPVYPTEIVFALDASQDVPPAAFQRMKNTIISIIEKLDISESNCPRGARVAVVAYNSNTKYLIRFSDYKRKSLLLEAIRGISPERSSSRRNIGEAMKFVGRNIFKRIRHGIHVRKVAVFFANGPSPDADAINSAVLEYSALDIIPIVIAFNNVPNVVAAFSADETRRFQVFVWSRPEDQRLELISYCTLCYDKCTPDEECELDQMPATLIDMDIAFVLDSSHNVQADVFEEMKDFVSSMLDTVYISTRPAVSETEARVALVQQAVKDFGPNRNTSPVKTEFDLLRYNSANMMKRHIKQSVRQLNGPSAVGNSVQWVIDNIFLKTPMPRKHKVLFLILASPTSYWDREKLKEVSKKAKCQGFAIFVLGFGKTISELEMTQLPSLPQDQHLLRLISMSKPELVYAKKFSHAFFNLLDRGFNHYPPAVLQAECQGRGDSSFSVVRSFPDTRLTQVDTAAAEVPDETIEVVEVLGTPAPEEIVGKKTEKIIINKGKKANGEVRSLKKDVCTMKQEEGKCADYILKWSYNQGKKACVQFWYGGCGGNGNRFDTQEQCEATCMS

Target ORF information:

RefSeq Version	XM_012965535.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen type VI alpha 5 chain (col6a5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965535.2

ORF Insert Sequence:

ATGGGATTTT GGAAAGTATT TCTCGCCTGC GTGCTTGGCA GCTACTCAGT CAATGTCTCA 
GCTTCTCAGA AAACAGTGTG CACAGAGGCC AGTATTGCAG ATATTGTATT CCTTATGGAT 
GGATCCTGGA GCATCGGAAC GGAAAACTTT ATCACAATGA AGAACTTTTT GTACACTTTG 
GTTAATGGTT TTGATGTTGG CCTAGATAAG ATCCAGATTG GCCTAATCCA GTACAGTGAT 
AATGCACGTA CAGAATTCTT CCTAAACAGT TACAGTAACA AAGAAGATGT TTTGAAATAT 
ATTCAGAATC TAAAGTATAA AGGGGGTGGG ACAAAGACTG GCTTAAGTCT GGAGTTCATG 
CTGACGCAGC ACTTTAGTGA AGCTGCAGGC AGCAGAGCAG CAGAAGGTGT GCCTCAGATT 
GCAGTCGTTA TAACTGATGG ACAAGCTCAA GATAGCATCA GGGAGCCTGC CATTGCAGTG 
AAGAACGCTG GAATCATACT GTATGCCATT GGCATTAAAG ATGCAGTTTT ATCTGAGCTT 
AATGAAATAG CCAGTGATCC AGATGACAAA CATGTTTATA GCGTTGCAGA CTTTAATGCA 
CTGCAAAGTA TTTCCCAAAA CATGATCCAG GTGCTCTGCA CAACTGTAGA AGAAGCAGCA 
AGACAGACAG GTCAAATTGC TCAAGTTTGC AGAACTGCCA ATCAAGCTGA CATTGTGCTG 
CTAGTCGAAA GCACAACAAG AATGGGTGAC GCTACTTTTG AAAAAGCAAA GAATTTTCTT 
TATGATTTAG TTTCTAATTT GGATGTTGGA ATTAATAAGA TTCGCATTGG GCTTGTAACC 
TACAATGATG AGACAAATCC AGAATTTTTG CTAAACAGCT ATTCTTCGAA AACTGAAATC 
TTAGAAAGTA TTCAGAATAT GAAATATGTA GAAGGATATA CCTACACAGG GAGAGCCCTT 
GAGTATGTAA ATACAACCTA CTTTACACAG GCAGCAGGCA GTAGATTTGA AGAGAGCGTC 
GCTCAAATTC TTATCATTGT GACAGAAGGA GACTCTTCAG ATACCCTCAC TGAGCCTGCT 
AAAGAACTCA AATCAAGAGG CATATCTGTT TATGTGGTTG GGACAAACAT CAAGTACGAT 
AGACAGCTTC AGGAAGCATC AAGCAAGCCC GACGAAAAGT TCTTTTATCA ACTCGATGAT 
TTTGATGACT CTGAGAATGT CACTGAGCAG CTGTTAAAAA ACCTGTGCTT TAGCATAGAT 
TTGAATATTC AAGTATATTC AAAGCGCTAC GCTGATGTTG TATTTCTAGT CGATTCTTCT 
ACAAGCATGG GAACCATCTT TTTTCAAAAG ATGAAGGATT TCATCATACA CATAATAAAC 
CAGTTAAATG TTGGAATAAA TAAACACCGT ATAGGACTGG CACAGTACAG TGGTCTACCC 
CAAACTGAAT TTCTACTGAA TCATTATGAA ACCAAAGAGG AGATACTAAA GCACATTAAG 
GAAACATTCA CATACAGAGG TGGCCCCTTA AAAACAGGAC ATGCTTTGGA ATTTGTACGC 
TCAACATTTT TCATAGAAGA GGCTGGGAGT AGGATTAACT ACGGCAACCC CCAGTTCTTA 
GTGGTCATAA CATCATCAAA ATCAGAGGAT GCTGTTAGAA GACATGCCGA GGAACTCAAA 
AGTGTTGGGG TCACAACCAT CTCTGTTGGC ATTGGCAATT CTGATCGAAA AGAACTGGAA 
AAAATTGCTA CTGATCCTTT TGTTTTCCAG ACCACAGGTT TACAGCACAT TTCAAATTTA 
CAGCAGGATG TGGCCAATGT TATTATAGCA GAAGACATGC TACAGTTTTC CCCAGTATCA 
GTTGTACCAG CAGTTTGCTC CAGTGCTTCT GTAGCTGACA TTGTGTTTCT AATTGATGAA 
TCTAGCAGCA TTGGCCCCAT CAACTTTCAA CTAACCAGAG TCTTTCTACA CAAGGTTGTT 
AGTGCACTGG ATATTTCCTT GAGTAATGTT AGAGTGGGCT TAGTGCTTTA CAGTGATGAG 
CCAAGACTTG AACTTAAACT GAACACTTTT AATGAAAAAT ATGAAATTTT GGACTTCATA 
ACTAAGCTGC CATACAGAGG AGGCAAAGCA CATACAGGTG CTGCGCTGGA CTTTCTAAGG 
AAAAAAATGT TCACCAAACA AAATGGCGGC CGGCCACATC AAGGAGTTCA GCAGATAGCG 
GTGGTTATGA CTAATGGGCA GTCCATGGAT AATTTTACTA AACCTGCTGC AAAACTGAGA 
CGGTCTGGCG TGGAGGTTTT TGCTGTTGGC TTTCAGAATA TCAATGATAC AGAACTGGAT 
ATCATAGCAT CTCACCCACC GCGAAAACAT GTCACAAATG TGGAATCTTT CCTGCAGCTA 
TCCAACCTTG AGTTCAGAAT ACAGAAACGA CTTTGCAATG AAATTGTTGT GAAGTCATTT 
GCAGTACCTG CAATTGCTCG TGCAGTGAAG GAAGGGTGTG TGGATACAGA AGAAGCAGAT 
ATATACTTTC TAATTGATGG TTCTGGCAGT ATATATCCAG AGGACTTTGA AGATATGAAA 
AAATTCATGA TTGAATTAAT AAGCATGTTT CAAGTTGGTG CCAACAGAGT ACGCTTTGGA 
GTTGTCCAAT ACTCAGATGT TCGTAGAACT GAATTTTTCA TCAGTGAACA CAACACCCAG 
AAAATGTTAA AGGATGCTAT TTCTCAAATA GAACAGTTGG GCGGTGGTAC CCTAACAGGA 
GAAGCTTTAA CGTCAATGAA GCAACTTTTT GTTAATGCAG CTAAAGATCG CCCTCATAAA 
GTACCCCAGT CATTGGTTGT CATTACAGAT GGAGAATCTC AAGACCGGGT CACAGAAGCA 
GCTGCAGAAA TTAGAAATGA TGGCATTACT ATATTTGCAA TTGGTGTTAA AAATGCAGTG 
GAAGAAGAGA TTAGAGATAT AGCAGGGTCA AATGAGAAGA TGTTTTTTGT TAACAACTTT 
GATTCCCTGA AGGTGATCAA GAATGATTTG GCTCGTGAAT TGTGTACACC TGAGGCATGT 
AAGAACATGA AAGCTGATAT CGTGTTTCTG GTTGATAGCT CTGCAAGCAT TAACAGTGAT 
GATTATGAAA CAATGAAAGA ATTCATGGAG TCAATGGTCA AGCAGGCAGA AATCGGCCCT 
GATAGAGTCC AGATTGGTTT AATTCAGTTT AGCTCTGAAA CAAAGGAAGA ATTTCCTCTC 
AACAGGTACA AAAGAAAGGA TGAAATACAA TCAGCAATAA GAGGCATCCA ACAACTGAGC 
CAGGGAACCC TTATGGGGGA AGCCCTTAAA TATACATTAC CTTATTTTAG TGCTTCCAAA 
GGAGGCCGTG TAAATACCAA ACAATATCTG ATCGTCATAA CAGATGGTGA GGCCCAGGAT 
GCTGTGGGAA ACCCAGCCAA AGCAATAAGG GACCATGGAG TTATCATTTA TGCCATAGGA 
GTACAACAAG CCAACAACAC ACAACTTCTG GAGATTGCTG GCAAACAAGA GCAGGTGTAT 
TATGAAGATA GCTTTGATTC CCTAGCTTTT TTGAACAAGA ACATCATGTT TGAGATTTGC 
AATCCTCAAG AATCTTGCAA AAAGACTGAG GTGGCAGACA TCATATTTCT GTTGGATGCT 
TCTGCAAGCA TAACCAGAGG AGAGTTTAGA CTCATGCAGA GATTTGTGGA GGCTGTTGTC 
AATGACTCTT TGGTTGGAAA GGATAATGTT CAATTTGGAG CAGTTGTTTA CGGCACAAAT 
CCAGCAGAAC AGTTTTCACT CAATACGTAT TCAACTAAAC TTGATATTCT TAAGGCTGTA 
TTTAGTTTAC CCCAAGTATC TGGATACACA TACACTGCAA AAGCGCTGGA ATACACTCGG 
ATACGGTTTG GTACATCATA TGGTGGAAGA CCAGGAATTT CACATATTCT AATTCTAGTC 
ACAGATGGAG CCACTACTGA AGCTGATCGC CCTAACCTAC CAATAGTATC AAAGGCTTTG 
AAGGATGATG GCATCATTGT CTTTGCAGTA GGAGTTGGCA AGGCAGTTCC TCAAGAGCTT 
CAGCAGATTG CAGGATACCC AGACAGATGG TTCTTAGTCC AGAACTACAA AGGCCTGGAC 
AATATACATG ATAATATAAC ACAGGTTGTA TGTGATGAAT CAAAACCAGC TTGCTCACAT 
GAACAGCTTG ATCTTGTTTT CCTGATTGAC GGCTCAGCAA GTATCACTTC TAGCAACTTC 
ACAAGTGCCA AGACTTTCAT GAAAGAAATC GTTGACAGCT TTACAATTTC AGAGAACAGA 
GTGCGTATTG GAGTCGCACA GTACAGTGCA AATCCCAAGA AAGAATTCTT CTTAAATGAG 
TACTACTCCA GCTCCGACAT GAAGAAACAG ATTGATAGCA TCTCCCAACT GAAAGCTACC 
ACTTACACTG GCAAAGGCCT ACGATTTGTC AAACAGTTCT TTGATCCAGC AAATGGCGGC 
CGTAAGAATG TTCCGCAGTA CCTGATAGTC ATGACTGATG GCATGTCTAA TGACAGCGTA 
AATGAAGATG CTGCTGCTTT GCGAAGCAGT GGAGTAAAGA TCTTTTCCAT TGGTATTGGT 
CTGCGGAACA GTTTTGAGCT TGTTATGATT GCCGGGTCAC CCAAAAATGT TTACGAAGTG 
GAAACGTTCC AAGCGCTGGA CTCTATAAAG AGGCAGATTG TGGCACAAGT CTGTGAGCCA 
TCTGATCAGC CTATTATAGG TAAAGAATGC AGCTTAGATA TCACCATATC CGTTGACTAC 
TCAAGAAGAG CAATGCTATC TACTGTCCCC CGTGTGCAGC AGAAATTGCA GCAGTTCCTC 
CCTCCGGTGC TTCAGCGCAT TTCCTCGCTT AGCAATATCA GCTGTGCCTC TGGATCTCAA 
ATTAACTTAC GCTTCAGATA CGCTGTTGCA GCACAGGACA GACTGACATT TGATTCCGAC 
TTTGAGAAAT TTAACGAGGA CATCATTAAG AAGTTTATAG CTGAACAGTC TTCTGTTGAT 
ACATATTTAA ATGCAAAGTT TCTTCAGTCC CTCTGGAACA AATACATAGG TTTGCCGTCT 
CAGAAAGCAA AAATTATTCT GGTTTTTACT GATGGACTGG ATGAAAGTTC TGAAGTACTC 
AAGGCGACTA CTGAATCTCT TCGGAAACAA GGGTTGGATG CACTGTTATT CATTGCTCTT 
GAGGGGGTGA AGGATTTTAC AGAGCTGCAA AACTTAGAGT TTGGAAGAGG ATTTTCTTAC 
CAGCAACCTC TGAGCATTGC GCTGCATGAT CTGCCAAGTG TAATGCTAAA AGAAATGGAT 
ACCGTTGCAG AAAGGAAATG CTGTAATGTA GTTTGTAAAT GTTTAGGACA AGATGGACCT 
CTAGGCTCAC CAGGGGCTTT AGGAACAAAG GGTCAGCCTG GTATAAAGGG ATCTCAAGGT 
CATCCTGGAG AGGAAGGCGG TCATGGTGAA AGAGGATTCC GTGGGCTGAA TGGGACAAGA 
GGCGAAAGTG GATGTCCTGG TCGTAGGGGA TTAAAGGGTG CCAGAGGTAT TCGTGGTGAC 
AAGGGTGATG ATGGAGAGTT TGGAATTGAT GGTGTGCCTG GAGAGCAGGG TGAACCTGGA 
GGGCGTGGAG CATCAGGAGA ACGTGGAGAC ACTGGAGCTC AGGGCAGAAA AGGTCCTCGT 
GGGCAACCTG GAGAAAAAGG GGAAAATGGA CTGAGGGGAG ACCCAGGTGA GCCTGGAACT 
AACATTAACA TTCAGGGACC TATAGGACTG AAAGGAAACC CAGGACGTCA GGGAGAGGCG 
GGAAGTGACG GTGTGGAAGG AGAGCCAGGA AATAATGGAC CTAATGGTCC ACAAGGCCGA 
AGAGGGCCTC CTGGTTTAAA GGGAGCACGA GGATTTCCTG GAGAGCCAGG GAACAAAGGT 
GATAGTGGAA TCCAAGGTAT ACAGGGTTCC CAAGGCATGC CTGGACCACC AGGTCCACAG 
GGACCACAAG GGTTATCTGG ACGTCAGGGA GATGCAGGTG CCCCTGGTGC ATCAGGTTCC 
TTTGGCAAAC CCGGAGCTCC AGGACTAAAG GGGGAACCTG GAGAAAACGG AGAGAAGGGT 
GAGCCAGGCA TTCCGGGTCG CAGAGGTTTG CCAGGGCTGG ATGGTCCTGA TGGATATGGT 
CCCCCAGGCT TAAAGGGAGA GAAGGGTCAT TTAGGGTTTC CTGGGGATCC TGGGCCTCAG 
GGAGAAGATG GTGATCCTGG AATAGCTGGA AATGGTGGCC CAAAAGGAAT TAGTGGAAGA 
AGGGGTAATT CTGGCCCTCC AGGAACTAAT GGACAAAGGG GTGAGAGGGG ACCACGTGGA 
CCTATGGGCG CCAAAGGATT GCCAGGAACT ATGCCTATGA CGCCTTGTGA ACTTGTGGCG 
TTTACTCGAG AGAATTGTCC TTGCTTCGCA AGTGCTTCAA AATGTCCAGT TTACCCAACT 
GAAATAGTGT TTGCTCTGGA TGCATCTCAG GACGTTCCCC CGGCTGCTTT CCAAAGGATG 
AAGAACACCA TTATTTCCAT AATAGAAAAG CTGGACATTA GTGAGAGCAA TTGCCCTAGA 
GGAGCCCGTG TTGCTGTCGT TGCGTATAAC AGTAATACCA AATACCTCAT TCGTTTCTCT 
GACTACAAAC GGAAAAGCCT ACTGCTGGAA GCAATTCGTG GCATCTCTCC AGAAAGATCA 
TCTTCAAGGA GAAATATTGG AGAAGCCATG AAGTTTGTAG GGAGGAACAT ATTCAAACGC 
ATTCGTCATG GAATCCATGT TAGAAAAGTG GCTGTGTTCT TTGCTAATGG CCCATCCCCA 
GATGCAGATG CAATAAACTC GGCAGTGCTG GAATACAGTG CTCTTGACAT TATTCCTATT 
GTGATTGCAT TTAATAATGT TCCCAATGTC GTAGCAGCTT TTTCGGCCGA CGAAACTAGA 
AGATTCCAAG TGTTTGTATG GAGTAGGCCT GAGGATCAGA GACTAGAACT TATTTCTTAT 
TGTACTCTTT GTTATGATAA GTGTACACCT GATGAAGAAT GTGAGCTGGA TCAAATGCCT 
GCAACCCTAA TAGATATGGA CATAGCTTTT GTATTAGACA GTTCACACAA TGTGCAAGCT 
GATGTCTTTG AGGAAATGAA AGACTTTGTG AGCAGTATGC TGGACACCGT CTACATATCA 
ACGCGGCCCG CAGTTTCTGA AACAGAAGCC AGAGTTGCCT TAGTACAACA GGCAGTTAAG 
GACTTTGGTC CAAATAGGAA TACTAGCCCA GTTAAAACTG AGTTCGACCT TCTTCGCTAC 
AACAGCGCAA ATATGATGAA GAGGCACATC AAACAATCTG TCAGGCAACT GAATGGTCCT 
TCTGCAGTTG GCAATTCTGT TCAGTGGGTC ATTGATAATA TCTTCTTAAA AACACCAATG 
CCAAGAAAGC ATAAAGTACT TTTCCTCATT CTTGCCAGCC CAACAAGCTA TTGGGACAGA 
GAAAAATTGA AAGAAGTCTC CAAAAAAGCT AAGTGTCAAG GATTTGCAAT ATTTGTACTG 
GGTTTTGGAA AAACGATAAG TGAGCTGGAA ATGACACAGC TGCCGAGCTT GCCACAAGAC 
CAACATTTGC TTCGCTTGAT AAGCATGTCA AAACCAGAAC TTGTCTATGC AAAGAAGTTC 
AGTCATGCCT TCTTCAATCT ACTCGACCGT GGCTTTAATC ACTACCCACC CGCAGTATTG 
CAAGCAGAGT GCCAAGGGCG TGGGGACTCA TCTTTTTCAG TTGTAAGAAG CTTCCCTGAC 
ACACGGTTGA CACAAGTTGA CACTGCTGCA GCAGAAGTTC CAGATGAGAC TATAGAAGTA 
GTGGAGGTCC TTGGTACACC TGCACCTGAA GAAATTGTGG GGAAAAAAAC AGAGAAAATA 
ATCATAAACA AAGGTAAAAA AGCTAATGGG GAAGTGCGGA GCCTTAAGAA AGATGTCTGT 
ACAATGAAAC AAGAGGAAGG AAAATGTGCA GATTACATCC TCAAGTGGTC CTACAACCAA 
GGCAAAAAAG CTTGTGTGCA GTTCTGGTAC GGTGGCTGTG GAGGAAATGG CAATCGTTTT 
GACACACAGG AACAGTGTGA AGCCACCTGC ATGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col6a5 ( XM_012965536.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820990.2 Xenopus tropicalis collagen type VI alpha 5 chain (col6a5), transcript variant X4, mRNA.

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CloneID	OXa59876
Clone ID Related Accession (Same CDS sequence)	XM_012965536.2
Accession Version	XM_012965536.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTTT GGAAAGTATT TCTCGCCTGC GTGCTTGGCA GCTACTCAGT CAATGTCTCA 
GCTTCTCAGA AAACAGTGTG CACAGAGGCC AGTATTGCAG ATATTGTATT CCTTATGGAT 
GGATCCTGGA GCATCGGAAC GGAAAACTTT ATCACAATGA AGAACTTTTT GTACACTTTG 
GTTAATGGTT TTGATGTTGG CCTAGATAAG ATCCAGATTG GCCTAATCCA GTACAGTGAT 
AATGCACGTA CAGAATTCTT CCTAAACAGT TACAGTAACA AAGAAGATGT TTTGAAATAT 
ATTCAGAATC TAAAGTATAA AGGGGGTGGG ACAAAGACTG GCTTAAGTCT GGAGTTCATG 
CTGACGCAGC ACTTTAGTGA AGCTGCAGGC AGCAGAGCAG CAGAAGGTGT GCCTCAGATT 
GCAGTCGTTA TAACTGATGG ACAAGCTCAA GATAGCATCA GGGAGCCTGC CATTGCAGTG 
AAGAACGCTG GAATCATACT GTATGCCATT GGCATTAAAG ATGCAGTTTT ATCTGAGCTT 
AATGAAATAG CCAGTGATCC AGATGACAAA CATGTTTATA GCGTTGCAGA CTTTAATGCA 
CTGCAAAGTA TTTCCCAAAA CATGATCCAG GTGCTCTGCA CAACTGTAGA AGAAGCAGCA 
AGACAGACAG GTCAAATTGC TCAAGTTTGC AGAACTGCCA ATCAAGCTGA CATTGTGCTG 
CTAGTCGAAA GCACAACAAG AATGGGTGAC GCTACTTTTG AAAAAGCAAA GAATTTTCTT 
TATGATTTAG TTTCTAATTT GGATGTTGGA ATTAATAAGA TTCGCATTGG GCTTGTAACC 
TACAATGATG AGACAAATCC AGAATTTTTG CTAAACAGCT ATTCTTCGAA AACTGAAATC 
TTAGAAAGTA TTCAGAATAT GAAATATGTA GAAGGATATA CCTACACAGG GAGAGCCCTT 
GAGTATGTAA ATACAACCTA CTTTACACAG GCAGCAGGCA GTAGATTTGA AGAGAGCGTC 
GCTCAAATTC TTATCATTGT GACAGAAGGA GACTCTTCAG ATACCCTCAC TGAGCCTGCT 
AAAGAACTCA AATCAAGAGG CATATCTGTT TATGTGGTTG GGACAAACAT CAAGTACGAT 
AGACAGCTTC AGGAAGCATC AAGCAAGCCC GACGAAAAGT TCTTTTATCA ACTCGATGAT 
TTTGATGACT CTGAGAATGT CACTGAGCAG CTGTTAAAAA ACCTGTGCTT TAGCATAGAT 
TTGAATATTC AAGTATATTC AAAGCGCTAC GCTGATGTTG TATTTCTAGT CGATTCTTCT 
ACAAGCATGG GAACCATCTT TTTTCAAAAG ATGAAGGATT TCATCATACA CATAATAAAC 
CAGTTAAATG TTGGAATAAA TAAACACCGT ATAGGACTGG CACAGTACAG TGGTCTACCC 
CAAACTGAAT TTCTACTGAA TCATTATGAA ACCAAAGAGG AGATACTAAA GCACATTAAG 
GAAACATTCA CATACAGAGG TGGCCCCTTA AAAACAGGAC ATGCTTTGGA ATTTGTACGC 
TCAACATTTT TCATAGAAGA GGCTGGGAGT AGGATTAACT ACGGCAACCC CCAGTTCTTA 
GTGGTCATAA CATCATCAAA ATCAGAGGAT GCTGTTAGAA GACATGCCGA GGAACTCAAA 
AGTGTTGGGG TCACAACCAT CTCTGTTGGC ATTGGCAATT CTGATCGAAA AGAACTGGAA 
AAAATTGCTA CTGATCCTTT TGTTTTCCAG ACCACAGGTT TACAGCACAT TTCAAATTTA 
CAGCAGGATG TGGCCAATGT TATTATAGCA GAAGACATGC TACAGTTTTC CCCAGTATCA 
GTTGTACCAG CAGTTTGCTC CAGTGCTTCT GTAGCTGACA TTGTGTTTCT AATTGATGAA 
TCTAGCAGCA TTGGCCCCAT CAACTTTCAA CTAACCAGAG TCTTTCTACA CAAGGTTGTT 
AGTGCACTGG ATATTTCCTT GAGTAATGTT AGAGTGGGCT TAGTGCTTTA CAGTGATGAG 
CCAAGACTTG AACTTAAACT GAACACTTTT AATGAAAAAT ATGAAATTTT GGACTTCATA 
ACTAAGCTGC CATACAGAGG AGGCAAAGCA CATACAGGTG CTGCGCTGGA CTTTCTAAGG 
AAAAAAATGT TCACCAAACA AAATGGCGGC CGGCCACATC AAGGAGTTCA GCAGATAGCG 
GTGGTTATGA CTAATGGGCA GTCCATGGAT AATTTTACTA AACCTGCTGC AAAACTGAGA 
CGGTCTGGCG TGGAGGTTTT TGCTGTTGGC TTTCAGAATA TCAATGATAC AGAACTGGAT 
ATCATAGCAT CTCACCCACC GCGAAAACAT GTCACAAATG TGGAATCTTT CCTGCAGCTA 
TCCAACCTTG AGTTCAGAAT ACAGAAACGA CTTTGCAATG AAATTGTTGT GAAGTCATTT 
GCAGTACCTG CAATTGCTCG TGCAGTGAAG GAAGGGTGTG TGGATACAGA AGAAGCAGAT 
ATATACTTTC TAATTGATGG TTCTGGCAGT ATATATCCAG AGGACTTTGA AGATATGAAA 
AAATTCATGA TTGAATTAAT AAGCATGTTT CAAGTTGGTG CCAACAGAGT ACGCTTTGGA 
GTTGTCCAAT ACTCAGATGT TCGTAGAACT GAATTTTTCA TCAGTGAACA CAACACCCAG 
AAAATGTTAA AGGATGCTAT TTCTCAAATA GAACAGTTGG GCGGTGGTAC CCTAACAGGA 
GAAGCTTTAA CGTCAATGAA GCAACTTTTT GTTAATGCAG CTAAAGATCG CCCTCATAAA 
GTACCCCAGT CATTGGTTGT CATTACAGAT GGAGAATCTC AAGACCGGGT CACAGAAGCA 
GCTGCAGAAA TTAGAAATGA TGGCATTACT ATATTTGCAA TTGGTGTTAA AAATGCAGTG 
GAAGAAGAGA TTAGAGATAT AGCAGGGTCA AATGAGAAGA TGTTTTTTGT TAACAACTTT 
GATTCCCTGA AGGTGATCAA GAATGATTTG GCTCGTGAAT TGTGTACACC TGAGGCATGT 
AAGAACATGA AAGCTGATAT CGTGTTTCTG GTTGATAGCT CTGCAAGCAT TAACAGTGAT 
GATTATGAAA CAATGAAAGA ATTCATGGAG TCAATGGTCA AGCAGGCAGA AATCGGCCCT 
GATAGAGTCC AGATTGGTTT AATTCAGTTT AGCTCTGAAA CAAAGGAAGA ATTTCCTCTC 
AACAGGTACA AAAGAAAGGA TGAAATACAA TCAGCAATAA GAGGCATCCA ACAACTGAGC 
CAGGGAACCC TTATGGGGGA AGCCCTTAAA TATACATTAC CTTATTTTAG TGCTTCCAAA 
GGAGGCCGTG TAAATACCAA ACAATATCTG ATCGTCATAA CAGATGGTGA GGCCCAGGAT 
GCTGTGGGAA ACCCAGCCAA AGCAATAAGG GACCATGGAG TTATCATTTA TGCCATAGGA 
GTACAACAAG CCAACAACAC ACAACTTCTG GAGATTGCTG GCAAACAAGA GCAGGTGTAT 
TATGAAGATA GCTTTGATTC CCTAGCTTTT TTGAACAAGA ACATCATGTT TGAGATTTGC 
AATCCTCAAG AATCTTGCAA AAAGACTGAG GTGGCAGACA TCATATTTCT GTTGGATGCT 
TCTGCAAGCA TAACCAGAGG AGAGTTTAGA CTCATGCAGA GATTTGTGGA GGCTGTTGTC 
AATGACTCTT TGGTTGGAAA GGATAATGTT CAATTTGGAG CAGTTGTTTA CGGCACAAAT 
CCAGCAGAAC AGTTTTCACT CAATACGTAT TCAACTAAAC TTGATATTCT TAAGGCTGTA 
TTTAGTTTAC CCCAAGTATC TGGATACACA TACACTGCAA AAGCGCTGGA ATACACTCGG 
ATACGGTTTG GTACATCATA TGGTGGAAGA CCAGGAATTT CACATATTCT AATTCTAGTC 
ACAGATGGAG CCACTACTGA AGCTGATCGC CCTAACCTAC CAATAGTATC AAAGGCTTTG 
AAGGATGATG GCATCATTGT CTTTGCAGTA GGAGTTGGCA AGGCAGTTCC TCAAGAGCTT 
CAGCAGATTG CAGGATACCC AGACAGATGG TTCTTAGTCC AGAACTACAA AGGCCTGGAC 
AATATACATG ATAATATAAC ACAGGTTGTA TGTGATGAAT CAAAACCAGC TTGCTCACAT 
GAACAGCTTG ATCTTGTTTT CCTGATTGAC GGCTCAGCAA GTATCACTTC TAGCAACTTC 
ACAAGTGCCA AGACTTTCAT GAAAGAAATC GTTGACAGCT TTACAATTTC AGAGAACAGA 
GTGCGTATTG GAGTCGCACA GTACAGTGCA AATCCCAAGA AAGAATTCTT CTTAAATGAG 
TACTACTCCA GCTCCGACAT GAAGAAACAG ATTGATAGCA TCTCCCAACT GAAAGCTACC 
ACTTACACTG GCAAAGGCCT ACGATTTGTC AAACAGTTCT TTGATCCAGC AAATGGCGGC 
CGTAAGAATG TTCCGCAGTA CCTGATAGTC ATGACTGATG GCATGTCTAA TGACAGCGTA 
AATGAAGATG CTGCTGCTTT GCGAAGCAGT GGAGTAAAGA TCTTTTCCAT TGGTATTGGT 
CTGCGGAACA GTTTTGAGCT TGTTATGATT GCCGGGTCAC CCAAAAATGT TTACGAAGTG 
GAAACGTTCC AAGCGCTGGA CTCTATAAAG AGGCAGATTG TGGCACAAGT CTGTGAGCCA 
TCTGATCAGC CTATTATAGG TAAAGAATGC AGCTTAGATA TCACCATATC CGTTGACTAC 
TCAAGAAGAG CAATGCTATC TACTGTCCCC CGTGTGCAGC AGAAATTGCA GCAGTTCCTC 
CCTCCGGTGC TTCAGCGCAT TTCCTCGCTT AGCAATATCA GCTGTGCCTC TGGATCTCAA 
ATTAACTTAC GCTTCAGATA CGCTGTTGCA GCACAGGACA GACTGACATT TGATTCCGAC 
TTTGAGAAAT TTAACGAGGA CATCATTAAG AAGTTTATAG CTGAACAGTC TTCTGTTGAT 
ACATATTTAA ATGCAAAGTT TCTTCAGTCC CTCTGGAACA AATACATAGG TTTGCCGTCT 
CAGAAAGCAA AAATTATTCT GGTTTTTACT GATGGACTGG ATGAAAGTTC TGAAGTACTC 
AAGGCGACTA CTGAATCTCT TCGGAAACAA GGGTTGGATG CACTGTTATT CATTGCTCTT 
GAGGGGGTGA AGGATTTTAC AGAGCTGCAA AACTTAGAGT TTGGAAGAGG ATTTTCTTAC 
CAGCAACCTC TGAGCATTGC GCTGCATGAT CTGCCAAGTG TAATGCTAAA AGAAATGGAT 
ACCGTTGCAG AAAGGAAATG CTGTAATGTA GTTTGTAAAT GTTTAGGACA AGATGGACCT 
CTAGGCTCAC CAGGGGCTTT AGGAACAAAG GGTCAGCCTG GTATAAAGGG ATCTCAAGGT 
CATCCTGGAG AGGAAGGCGG TCATGGTGAA AGAGGATTCC GTGGGCTGAA TGGGACAAGA 
GGCGAAAGTG GATGTCCTGG TCGTAGGGGA TTAAAGGGTG CCAGAGGTAT TCGTGGTGAC 
AAGGGTGATG ATGGAGAGTT TGGAATTGAT GGTGTGCCTG GAGAGCAGGG TGAACCTGGA 
GGGCGTGGAG CATCAGGAGA ACGTGGAGAC ACTGGAGCTC AGGGCAGAAA AGGTCCTCGT 
GGGCAACCTG GAGAAAAAGG GGAAAATGGA CTGAGGGGAG ACCCAGGTGA GCCTGGAACT 
AACATTAACA TTCAGGGACC TATAGGACTG AAAGGAAACC CAGGACGTCA GGGAGAGGCG 
GGAAGTGACG GTGTGGAAGG AGAGCCAGGA AATAATGGAC CTAATGGTCC ACAAGGCCGA 
AGAGGGCCTC CTGGTTTAAA GGGAGCACGA GGATTTCCTG GAGAGCCAGG GAACAAAGGT 
GATAGTGGAA TCCAAGGTAT ACAGGGTTCC CAAGGCATGC CTGGACCACC AGGTCCACAG 
GGACCACAAG GGTTATCTGG ACGTCAGGGA GATGCAGGTG CCCCTGGTGC ATCAGGTTCC 
TTTGGCAAAC CCGGAGCTCC AGGACTAAAG GGGGAACCTG GAGAAAACGG AGAGAAGGGT 
GAGCCAGGCA TTCCGGGTCG CAGAGGTTTG CCAGGGCTGG ATGGTCCTGA TGGATATGGT 
CCCCCAGGCT TAAAGGGAGA GAAGGGTCAT TTAGGGTTTC CTGGGGATCC TGGGCCTCAG 
GGAGAAGATG GTGATCCTGG AATAGCTGGA AATGGTGGCC CAAAAGGAAT TAGTGGAAGA 
AGGGGTAATT CTGGCCCTCC AGGAACTAAT GGACAAAGGG GTGAGAGGGG ACCACGTGGA 
CCTATGGGCG CCAAAGGATT GCCAGGAACT ATGCCTATGA CGCCTTGTGA ACTTGTGGCG 
TTTACTCGAG AGAATTGTCC TTGCTTCGCA AGTGCTTCAA AATGTCCAGT TTACCCAACT 
GAAATAGTGT TTGCTCTGGA TGCATCTCAG GACGTTCCCC CGGCTGCTTT CCAAAGGATG 
AAGAACACCA TTATTTCCAT AATAGAAAAG CTGGACATTA GTGAGAGCAA TTGCCCTAGA 
GGAGCCCGTG TTGCTGTCGT TGCGTATAAC AGTAATACCA AATACCTCAT TCGTTTCTCT 
GACTACAAAC GGAAAAGCCT ACTGCTGGAA GCAATTCGTG GCATCTCTCC AGAAAGATCA 
TCTTCAAGGA GAAATATTGG AGAAGCCATG AAGTTTGTAG GGAGGAACAT ATTCAAACGC 
ATTCGTCATG GAATCCATGT TAGAAAAGTG GCTGTGTTCT TTGCTAATGG CCCATCCCCA 
GATGCAGATG CAATAAACTC GGCAGTGCTG GAATACAGTG CTCTTGACAT TATTCCTATT 
GTGATTGCAT TTAATAATGT TCCCAATGTC GTAGCAGCTT TTTCGGCCGA CGAAACTAGA 
AGATTCCAAG TGTTTGTATG GAGTAGGCCT GAGGATCAGA GACTAGAACT TATTTCTTAT 
TGTACTCTTT GTTATGATAA GTGTACACCT GATGAAGAAT GTGAGCTGGA TCAAATGCCT 
GCAACCCTAA TAGATATGGA CATAGCTTTT GTATTAGACA GTTCACACAA TGTGCAAGCT 
GATGTCTTTG AGGAAATGAA AGACTTTGTG AGCAGTATGC TGGACACCGT CTACATATCA 
ACGCGGCCCG CAGTTTCTGA AACAGAAGCC AGAGTTGCCT TAGTACAACA GGCAGTTAAG 
GACTTTGGTC CAAATAGGAA TACTAGCCCA GTTAAAACTG AGTTCGACCT TCTTCGCTAC 
AACAGCGCAA ATATGATGAA GAGGCACATC AAACAATCTG TCAGGCAACT GAATGGTCCT 
TCTGCAGTTG GCAATTCTGT TCAGTGGGTC ATTGATAATA TCTTCTTAAA AACACCAATG 
CCAAGAAAGC ATAAAGTACT TTTCCTCATT CTTGCCAGCC CAACAAGCTA TTGGGACAGA 
GAAAAATTGA AAGAAGTCTC CAAAAAAGCT AAGTGTCAAG GATTTGCAAT ATTTGTACTG 
GGTTTTGGAA AAACGATAAG TGAGCTGGAA ATGACACAGC TGCCGAGCTT GCCACAAGAC 
CAACATTTGC TTCGCTTGAT AAGCATGTCA AAACCAGAAC TTGTCTATGC AAAGAAGTTC 
AGTCATGCCT TCTTCAATCT ACTCGACCGT GGCTTTAATC ACTACCCACC CGCAGTATTG 
CAAGCAGAGT GCCAAGGGCG TGGGGACTCA TCTTTTTCAG TTGTAAGAAG CTTCCCTGAC 
ACACGGTTGA CACAAGTTGA CACTGCTGCA GCAGAAGTTC CAGATGAGAC TATAGAAGTA 
GTGGAGGTCC TTGGTACACC TGCACCTGAA GAAATTGTGG GGAAAAAAAC AGAGAAAATA 
ATCATAAACA AAGATGTCTG TACAATGAAA CAAGAGGAAG GAAAATGTGC AGATTACATC 
CTCAAGTGGT CCTACAACCA AGGCAAAAAA GCTTGTGTGC AGTTCTGGTA CGGTGGCTGT 
GGAGGAAATG GCAATCGTTT TGACACACAG GAACAGTGTG AAGCCACCTG CATGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820990.2
CDS	131..8230
Translation	MGFWKVFLACVLGSYSVNVSASQKTVCTEASIADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREPAIAVKNAGIILYAIGIKDAVLSELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEPAKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRRHAEELKSVGVTTISVGIGNSDRKELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKPAAKLRRSGVEVFAVGFQNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELCTPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNPAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNPQESCKKTEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVPQELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVNEDAAALRSSGVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQVCEPSDQPIIGKECSLDITISVDYSRRAMLSTVPRVQQKLQQFLPPVLQRISSLSNISCASGSQINLRFRYAVAAQDRLTFDSDFEKFNEDIIKKFIAEQSSVDTYLNAKFLQSLWNKYIGLPSQKAKIILVFTDGLDESSEVLKATTESLRKQGLDALLFIALEGVKDFTELQNLEFGRGFSYQQPLSIALHDLPSVMLKEMDTVAERKCCNVVCKCLGQDGPLGSPGALGTKGQPGIKGSQGHPGEEGGHGERGFRGLNGTRGESGCPGRRGLKGARGIRGDKGDDGEFGIDGVPGEQGEPGGRGASGERGDTGAQGRKGPRGQPGEKGENGLRGDPGEPGTNINIQGPIGLKGNPGRQGEAGSDGVEGEPGNNGPNGPQGRRGPPGLKGARGFPGEPGNKGDSGIQGIQGSQGMPGPPGPQGPQGLSGRQGDAGAPGASGSFGKPGAPGLKGEPGENGEKGEPGIPGRRGLPGLDGPDGYGPPGLKGEKGHLGFPGDPGPQGEDGDPGIAGNGGPKGISGRRGNSGPPGTNGQRGERGPRGPMGAKGLPGTMPMTPCELVAFTRENCPCFASASKCPVYPTEIVFALDASQDVPPAAFQRMKNTIISIIEKLDISESNCPRGARVAVVAYNSNTKYLIRFSDYKRKSLLLEAIRGISPERSSSRRNIGEAMKFVGRNIFKRIRHGIHVRKVAVFFANGPSPDADAINSAVLEYSALDIIPIVIAFNNVPNVVAAFSADETRRFQVFVWSRPEDQRLELISYCTLCYDKCTPDEECELDQMPATLIDMDIAFVLDSSHNVQADVFEEMKDFVSSMLDTVYISTRPAVSETEARVALVQQAVKDFGPNRNTSPVKTEFDLLRYNSANMMKRHIKQSVRQLNGPSAVGNSVQWVIDNIFLKTPMPRKHKVLFLILASPTSYWDREKLKEVSKKAKCQGFAIFVLGFGKTISELEMTQLPSLPQDQHLLRLISMSKPELVYAKKFSHAFFNLLDRGFNHYPPAVLQAECQGRGDSSFSVVRSFPDTRLTQVDTAAAEVPDETIEVVEVLGTPAPEEIVGKKTEKIIINKDVCTMKQEEGKCADYILKWSYNQGKKACVQFWYGGCGGNGNRFDTQEQCEATCMS

Target ORF information:

RefSeq Version	XM_012965536.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen type VI alpha 5 chain (col6a5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965536.2

ORF Insert Sequence:

ATGGGATTTT GGAAAGTATT TCTCGCCTGC GTGCTTGGCA GCTACTCAGT CAATGTCTCA 
GCTTCTCAGA AAACAGTGTG CACAGAGGCC AGTATTGCAG ATATTGTATT CCTTATGGAT 
GGATCCTGGA GCATCGGAAC GGAAAACTTT ATCACAATGA AGAACTTTTT GTACACTTTG 
GTTAATGGTT TTGATGTTGG CCTAGATAAG ATCCAGATTG GCCTAATCCA GTACAGTGAT 
AATGCACGTA CAGAATTCTT CCTAAACAGT TACAGTAACA AAGAAGATGT TTTGAAATAT 
ATTCAGAATC TAAAGTATAA AGGGGGTGGG ACAAAGACTG GCTTAAGTCT GGAGTTCATG 
CTGACGCAGC ACTTTAGTGA AGCTGCAGGC AGCAGAGCAG CAGAAGGTGT GCCTCAGATT 
GCAGTCGTTA TAACTGATGG ACAAGCTCAA GATAGCATCA GGGAGCCTGC CATTGCAGTG 
AAGAACGCTG GAATCATACT GTATGCCATT GGCATTAAAG ATGCAGTTTT ATCTGAGCTT 
AATGAAATAG CCAGTGATCC AGATGACAAA CATGTTTATA GCGTTGCAGA CTTTAATGCA 
CTGCAAAGTA TTTCCCAAAA CATGATCCAG GTGCTCTGCA CAACTGTAGA AGAAGCAGCA 
AGACAGACAG GTCAAATTGC TCAAGTTTGC AGAACTGCCA ATCAAGCTGA CATTGTGCTG 
CTAGTCGAAA GCACAACAAG AATGGGTGAC GCTACTTTTG AAAAAGCAAA GAATTTTCTT 
TATGATTTAG TTTCTAATTT GGATGTTGGA ATTAATAAGA TTCGCATTGG GCTTGTAACC 
TACAATGATG AGACAAATCC AGAATTTTTG CTAAACAGCT ATTCTTCGAA AACTGAAATC 
TTAGAAAGTA TTCAGAATAT GAAATATGTA GAAGGATATA CCTACACAGG GAGAGCCCTT 
GAGTATGTAA ATACAACCTA CTTTACACAG GCAGCAGGCA GTAGATTTGA AGAGAGCGTC 
GCTCAAATTC TTATCATTGT GACAGAAGGA GACTCTTCAG ATACCCTCAC TGAGCCTGCT 
AAAGAACTCA AATCAAGAGG CATATCTGTT TATGTGGTTG GGACAAACAT CAAGTACGAT 
AGACAGCTTC AGGAAGCATC AAGCAAGCCC GACGAAAAGT TCTTTTATCA ACTCGATGAT 
TTTGATGACT CTGAGAATGT CACTGAGCAG CTGTTAAAAA ACCTGTGCTT TAGCATAGAT 
TTGAATATTC AAGTATATTC AAAGCGCTAC GCTGATGTTG TATTTCTAGT CGATTCTTCT 
ACAAGCATGG GAACCATCTT TTTTCAAAAG ATGAAGGATT TCATCATACA CATAATAAAC 
CAGTTAAATG TTGGAATAAA TAAACACCGT ATAGGACTGG CACAGTACAG TGGTCTACCC 
CAAACTGAAT TTCTACTGAA TCATTATGAA ACCAAAGAGG AGATACTAAA GCACATTAAG 
GAAACATTCA CATACAGAGG TGGCCCCTTA AAAACAGGAC ATGCTTTGGA ATTTGTACGC 
TCAACATTTT TCATAGAAGA GGCTGGGAGT AGGATTAACT ACGGCAACCC CCAGTTCTTA 
GTGGTCATAA CATCATCAAA ATCAGAGGAT GCTGTTAGAA GACATGCCGA GGAACTCAAA 
AGTGTTGGGG TCACAACCAT CTCTGTTGGC ATTGGCAATT CTGATCGAAA AGAACTGGAA 
AAAATTGCTA CTGATCCTTT TGTTTTCCAG ACCACAGGTT TACAGCACAT TTCAAATTTA 
CAGCAGGATG TGGCCAATGT TATTATAGCA GAAGACATGC TACAGTTTTC CCCAGTATCA 
GTTGTACCAG CAGTTTGCTC CAGTGCTTCT GTAGCTGACA TTGTGTTTCT AATTGATGAA 
TCTAGCAGCA TTGGCCCCAT CAACTTTCAA CTAACCAGAG TCTTTCTACA CAAGGTTGTT 
AGTGCACTGG ATATTTCCTT GAGTAATGTT AGAGTGGGCT TAGTGCTTTA CAGTGATGAG 
CCAAGACTTG AACTTAAACT GAACACTTTT AATGAAAAAT ATGAAATTTT GGACTTCATA 
ACTAAGCTGC CATACAGAGG AGGCAAAGCA CATACAGGTG CTGCGCTGGA CTTTCTAAGG 
AAAAAAATGT TCACCAAACA AAATGGCGGC CGGCCACATC AAGGAGTTCA GCAGATAGCG 
GTGGTTATGA CTAATGGGCA GTCCATGGAT AATTTTACTA AACCTGCTGC AAAACTGAGA 
CGGTCTGGCG TGGAGGTTTT TGCTGTTGGC TTTCAGAATA TCAATGATAC AGAACTGGAT 
ATCATAGCAT CTCACCCACC GCGAAAACAT GTCACAAATG TGGAATCTTT CCTGCAGCTA 
TCCAACCTTG AGTTCAGAAT ACAGAAACGA CTTTGCAATG AAATTGTTGT GAAGTCATTT 
GCAGTACCTG CAATTGCTCG TGCAGTGAAG GAAGGGTGTG TGGATACAGA AGAAGCAGAT 
ATATACTTTC TAATTGATGG TTCTGGCAGT ATATATCCAG AGGACTTTGA AGATATGAAA 
AAATTCATGA TTGAATTAAT AAGCATGTTT CAAGTTGGTG CCAACAGAGT ACGCTTTGGA 
GTTGTCCAAT ACTCAGATGT TCGTAGAACT GAATTTTTCA TCAGTGAACA CAACACCCAG 
AAAATGTTAA AGGATGCTAT TTCTCAAATA GAACAGTTGG GCGGTGGTAC CCTAACAGGA 
GAAGCTTTAA CGTCAATGAA GCAACTTTTT GTTAATGCAG CTAAAGATCG CCCTCATAAA 
GTACCCCAGT CATTGGTTGT CATTACAGAT GGAGAATCTC AAGACCGGGT CACAGAAGCA 
GCTGCAGAAA TTAGAAATGA TGGCATTACT ATATTTGCAA TTGGTGTTAA AAATGCAGTG 
GAAGAAGAGA TTAGAGATAT AGCAGGGTCA AATGAGAAGA TGTTTTTTGT TAACAACTTT 
GATTCCCTGA AGGTGATCAA GAATGATTTG GCTCGTGAAT TGTGTACACC TGAGGCATGT 
AAGAACATGA AAGCTGATAT CGTGTTTCTG GTTGATAGCT CTGCAAGCAT TAACAGTGAT 
GATTATGAAA CAATGAAAGA ATTCATGGAG TCAATGGTCA AGCAGGCAGA AATCGGCCCT 
GATAGAGTCC AGATTGGTTT AATTCAGTTT AGCTCTGAAA CAAAGGAAGA ATTTCCTCTC 
AACAGGTACA AAAGAAAGGA TGAAATACAA TCAGCAATAA GAGGCATCCA ACAACTGAGC 
CAGGGAACCC TTATGGGGGA AGCCCTTAAA TATACATTAC CTTATTTTAG TGCTTCCAAA 
GGAGGCCGTG TAAATACCAA ACAATATCTG ATCGTCATAA CAGATGGTGA GGCCCAGGAT 
GCTGTGGGAA ACCCAGCCAA AGCAATAAGG GACCATGGAG TTATCATTTA TGCCATAGGA 
GTACAACAAG CCAACAACAC ACAACTTCTG GAGATTGCTG GCAAACAAGA GCAGGTGTAT 
TATGAAGATA GCTTTGATTC CCTAGCTTTT TTGAACAAGA ACATCATGTT TGAGATTTGC 
AATCCTCAAG AATCTTGCAA AAAGACTGAG GTGGCAGACA TCATATTTCT GTTGGATGCT 
TCTGCAAGCA TAACCAGAGG AGAGTTTAGA CTCATGCAGA GATTTGTGGA GGCTGTTGTC 
AATGACTCTT TGGTTGGAAA GGATAATGTT CAATTTGGAG CAGTTGTTTA CGGCACAAAT 
CCAGCAGAAC AGTTTTCACT CAATACGTAT TCAACTAAAC TTGATATTCT TAAGGCTGTA 
TTTAGTTTAC CCCAAGTATC TGGATACACA TACACTGCAA AAGCGCTGGA ATACACTCGG 
ATACGGTTTG GTACATCATA TGGTGGAAGA CCAGGAATTT CACATATTCT AATTCTAGTC 
ACAGATGGAG CCACTACTGA AGCTGATCGC CCTAACCTAC CAATAGTATC AAAGGCTTTG 
AAGGATGATG GCATCATTGT CTTTGCAGTA GGAGTTGGCA AGGCAGTTCC TCAAGAGCTT 
CAGCAGATTG CAGGATACCC AGACAGATGG TTCTTAGTCC AGAACTACAA AGGCCTGGAC 
AATATACATG ATAATATAAC ACAGGTTGTA TGTGATGAAT CAAAACCAGC TTGCTCACAT 
GAACAGCTTG ATCTTGTTTT CCTGATTGAC GGCTCAGCAA GTATCACTTC TAGCAACTTC 
ACAAGTGCCA AGACTTTCAT GAAAGAAATC GTTGACAGCT TTACAATTTC AGAGAACAGA 
GTGCGTATTG GAGTCGCACA GTACAGTGCA AATCCCAAGA AAGAATTCTT CTTAAATGAG 
TACTACTCCA GCTCCGACAT GAAGAAACAG ATTGATAGCA TCTCCCAACT GAAAGCTACC 
ACTTACACTG GCAAAGGCCT ACGATTTGTC AAACAGTTCT TTGATCCAGC AAATGGCGGC 
CGTAAGAATG TTCCGCAGTA CCTGATAGTC ATGACTGATG GCATGTCTAA TGACAGCGTA 
AATGAAGATG CTGCTGCTTT GCGAAGCAGT GGAGTAAAGA TCTTTTCCAT TGGTATTGGT 
CTGCGGAACA GTTTTGAGCT TGTTATGATT GCCGGGTCAC CCAAAAATGT TTACGAAGTG 
GAAACGTTCC AAGCGCTGGA CTCTATAAAG AGGCAGATTG TGGCACAAGT CTGTGAGCCA 
TCTGATCAGC CTATTATAGG TAAAGAATGC AGCTTAGATA TCACCATATC CGTTGACTAC 
TCAAGAAGAG CAATGCTATC TACTGTCCCC CGTGTGCAGC AGAAATTGCA GCAGTTCCTC 
CCTCCGGTGC TTCAGCGCAT TTCCTCGCTT AGCAATATCA GCTGTGCCTC TGGATCTCAA 
ATTAACTTAC GCTTCAGATA CGCTGTTGCA GCACAGGACA GACTGACATT TGATTCCGAC 
TTTGAGAAAT TTAACGAGGA CATCATTAAG AAGTTTATAG CTGAACAGTC TTCTGTTGAT 
ACATATTTAA ATGCAAAGTT TCTTCAGTCC CTCTGGAACA AATACATAGG TTTGCCGTCT 
CAGAAAGCAA AAATTATTCT GGTTTTTACT GATGGACTGG ATGAAAGTTC TGAAGTACTC 
AAGGCGACTA CTGAATCTCT TCGGAAACAA GGGTTGGATG CACTGTTATT CATTGCTCTT 
GAGGGGGTGA AGGATTTTAC AGAGCTGCAA AACTTAGAGT TTGGAAGAGG ATTTTCTTAC 
CAGCAACCTC TGAGCATTGC GCTGCATGAT CTGCCAAGTG TAATGCTAAA AGAAATGGAT 
ACCGTTGCAG AAAGGAAATG CTGTAATGTA GTTTGTAAAT GTTTAGGACA AGATGGACCT 
CTAGGCTCAC CAGGGGCTTT AGGAACAAAG GGTCAGCCTG GTATAAAGGG ATCTCAAGGT 
CATCCTGGAG AGGAAGGCGG TCATGGTGAA AGAGGATTCC GTGGGCTGAA TGGGACAAGA 
GGCGAAAGTG GATGTCCTGG TCGTAGGGGA TTAAAGGGTG CCAGAGGTAT TCGTGGTGAC 
AAGGGTGATG ATGGAGAGTT TGGAATTGAT GGTGTGCCTG GAGAGCAGGG TGAACCTGGA 
GGGCGTGGAG CATCAGGAGA ACGTGGAGAC ACTGGAGCTC AGGGCAGAAA AGGTCCTCGT 
GGGCAACCTG GAGAAAAAGG GGAAAATGGA CTGAGGGGAG ACCCAGGTGA GCCTGGAACT 
AACATTAACA TTCAGGGACC TATAGGACTG AAAGGAAACC CAGGACGTCA GGGAGAGGCG 
GGAAGTGACG GTGTGGAAGG AGAGCCAGGA AATAATGGAC CTAATGGTCC ACAAGGCCGA 
AGAGGGCCTC CTGGTTTAAA GGGAGCACGA GGATTTCCTG GAGAGCCAGG GAACAAAGGT 
GATAGTGGAA TCCAAGGTAT ACAGGGTTCC CAAGGCATGC CTGGACCACC AGGTCCACAG 
GGACCACAAG GGTTATCTGG ACGTCAGGGA GATGCAGGTG CCCCTGGTGC ATCAGGTTCC 
TTTGGCAAAC CCGGAGCTCC AGGACTAAAG GGGGAACCTG GAGAAAACGG AGAGAAGGGT 
GAGCCAGGCA TTCCGGGTCG CAGAGGTTTG CCAGGGCTGG ATGGTCCTGA TGGATATGGT 
CCCCCAGGCT TAAAGGGAGA GAAGGGTCAT TTAGGGTTTC CTGGGGATCC TGGGCCTCAG 
GGAGAAGATG GTGATCCTGG AATAGCTGGA AATGGTGGCC CAAAAGGAAT TAGTGGAAGA 
AGGGGTAATT CTGGCCCTCC AGGAACTAAT GGACAAAGGG GTGAGAGGGG ACCACGTGGA 
CCTATGGGCG CCAAAGGATT GCCAGGAACT ATGCCTATGA CGCCTTGTGA ACTTGTGGCG 
TTTACTCGAG AGAATTGTCC TTGCTTCGCA AGTGCTTCAA AATGTCCAGT TTACCCAACT 
GAAATAGTGT TTGCTCTGGA TGCATCTCAG GACGTTCCCC CGGCTGCTTT CCAAAGGATG 
AAGAACACCA TTATTTCCAT AATAGAAAAG CTGGACATTA GTGAGAGCAA TTGCCCTAGA 
GGAGCCCGTG TTGCTGTCGT TGCGTATAAC AGTAATACCA AATACCTCAT TCGTTTCTCT 
GACTACAAAC GGAAAAGCCT ACTGCTGGAA GCAATTCGTG GCATCTCTCC AGAAAGATCA 
TCTTCAAGGA GAAATATTGG AGAAGCCATG AAGTTTGTAG GGAGGAACAT ATTCAAACGC 
ATTCGTCATG GAATCCATGT TAGAAAAGTG GCTGTGTTCT TTGCTAATGG CCCATCCCCA 
GATGCAGATG CAATAAACTC GGCAGTGCTG GAATACAGTG CTCTTGACAT TATTCCTATT 
GTGATTGCAT TTAATAATGT TCCCAATGTC GTAGCAGCTT TTTCGGCCGA CGAAACTAGA 
AGATTCCAAG TGTTTGTATG GAGTAGGCCT GAGGATCAGA GACTAGAACT TATTTCTTAT 
TGTACTCTTT GTTATGATAA GTGTACACCT GATGAAGAAT GTGAGCTGGA TCAAATGCCT 
GCAACCCTAA TAGATATGGA CATAGCTTTT GTATTAGACA GTTCACACAA TGTGCAAGCT 
GATGTCTTTG AGGAAATGAA AGACTTTGTG AGCAGTATGC TGGACACCGT CTACATATCA 
ACGCGGCCCG CAGTTTCTGA AACAGAAGCC AGAGTTGCCT TAGTACAACA GGCAGTTAAG 
GACTTTGGTC CAAATAGGAA TACTAGCCCA GTTAAAACTG AGTTCGACCT TCTTCGCTAC 
AACAGCGCAA ATATGATGAA GAGGCACATC AAACAATCTG TCAGGCAACT GAATGGTCCT 
TCTGCAGTTG GCAATTCTGT TCAGTGGGTC ATTGATAATA TCTTCTTAAA AACACCAATG 
CCAAGAAAGC ATAAAGTACT TTTCCTCATT CTTGCCAGCC CAACAAGCTA TTGGGACAGA 
GAAAAATTGA AAGAAGTCTC CAAAAAAGCT AAGTGTCAAG GATTTGCAAT ATTTGTACTG 
GGTTTTGGAA AAACGATAAG TGAGCTGGAA ATGACACAGC TGCCGAGCTT GCCACAAGAC 
CAACATTTGC TTCGCTTGAT AAGCATGTCA AAACCAGAAC TTGTCTATGC AAAGAAGTTC 
AGTCATGCCT TCTTCAATCT ACTCGACCGT GGCTTTAATC ACTACCCACC CGCAGTATTG 
CAAGCAGAGT GCCAAGGGCG TGGGGACTCA TCTTTTTCAG TTGTAAGAAG CTTCCCTGAC 
ACACGGTTGA CACAAGTTGA CACTGCTGCA GCAGAAGTTC CAGATGAGAC TATAGAAGTA 
GTGGAGGTCC TTGGTACACC TGCACCTGAA GAAATTGTGG GGAAAAAAAC AGAGAAAATA 
ATCATAAACA AAGATGTCTG TACAATGAAA CAAGAGGAAG GAAAATGTGC AGATTACATC 
CTCAAGTGGT CCTACAACCA AGGCAAAAAA GCTTGTGTGC AGTTCTGGTA CGGTGGCTGT 
GGAGGAAATG GCAATCGTTT TGACACACAG GAACAGTGTG AAGCCACCTG CATGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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col6a5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

サポート

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