COL5A2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL5A2
Entrez Gene ID	1290
Full Name	collagen type V alpha 2 chain
Synonyms	EDSC,EDSCL2
General protein information	Preferred Names collagen type V alpha 2 chain Names collagen alpha-2(V) chain AB collagen collagen, fetal membrane, A polypeptide type V preprocollagen alpha 2 chain
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q32.2
Summary	This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [provided by RefSeq, Jul 2008].
Disorder MIM:	120190
Disorder Html:	Ehlers-Danlos syndrome, classic type, 130000 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000393.4	NP_000384.2	collagen alpha-2(V) chain preproprotein
XM_011510573.2	XP_011508875.1	collagen alpha-2(V) chain isoform X1
NM_000393.5	NP_000384.2	collagen alpha-2(V) chain preproprotein
XM_011510573.3	XP_011508875.1	collagen alpha-2(V) chain isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	COL5A2	NP_000384.2
Pan troglodytes (chimpanzee)	COL5A2	XP_001164152.1
Canis lupus familiaris (dog)	COL5A2	XP_005640450.1
Rattus norvegicus (Norway rat)	Col5a2	NP_445940.1
Xenopus tropicalis (tropical clawed frog)	col5a2	XP_002931546.2
Danio rerio (zebrafish)	col5a2a	NP_001139254.1
Gallus gallus (chicken)	COL5A2	XP_004942453.1
	COL5A2	XP_002799008.1
Mus musculus (house mouse)	Col5a2	NP_031763.2

Gene Ontology
Bibliography

Related articles in PubMed

Spectrum of mucocutaneous, ocular and facial features and delineation of novel presentations in 62 classical Ehlers-Danlos syndrome patients.
Colombi M, Dordoni C, Venturini M, Ciaccio C, Morlino S, Chiarelli N, Zanca A, Calzavara-Pinton P, Zoppi N, Castori M, Ritelli M
Clinical genetics92(6)624-631(2017 Dec)

Proteomic characterization of human multiple myeloma bone marrow extracellular matrix.
Glavey SV, Naba A, Manier S, Clauser K, Tahri S, Park J, Reagan MR, Moschetta M, Mishima Y, Gambella M, Rocci A, Sacco A, O'Dwyer ME, Asara JM, Palumbo A, Roccaro AM, Hynes RO, Ghobrial IM
Leukemia31(11)2426-2434(2017 Nov)

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Naba A, Pearce OMT, Del Rosario A, Ma D, Ding H, Rajeeve V, Cutillas PR, Balkwill FR, Hynes RO
Journal of proteome research16(8)3083-3091(2017 Aug)

Comprehensive proteomic characterization of stem cell-derived extracellular matrices.
Ragelle H, Naba A, Larson BL, Zhou F, Priji? M, Whittaker CA, Del Rosario A, Langer R, Hynes RO, Anderson DG
Biomaterials128147-159(2017 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

COL5A2(+/-) humans, although unlikely to present with frank classic Ehlers-Danlos syndrome, are likely to have fragile connective tissues with increased susceptibility to trauma and certain chronic pathologic conditions.
Title: Homozygosity and Heterozygosity for Null Col5a2 Alleles Produce Embryonic Lethality and a Novel Classic Ehlers-Danlos Syndrome-Related Phenotype.

data confirm that COL5A1 and COL5A2 are the major, if not the only, genes involved in classic Ehlers-danlos syndrome
Title: Clinical and molecular characterization of 40 patients with classic Ehlers-Danlos syndrome: identification of 18 COL5A1 and 2 COL5A2 novel mutations.

Col5a2 shows predictive potential in myocardial infarction , and in principle may represent a novel candidate marker for the identification and treatment of ischemic cardiovascular disease
Title: Analysis of a gene co-expression network establishes robust association between Col5a2 and ischemic heart disease.

study shows that over 90% of patients, which strictly satisfy all major Villefranche criteria for classic Ehlers-Danlos Syndrome (EDS)harbor a type V collagen defect which indicates that this is the major--if not only--cause of classic EDS
Title: Comprehensive molecular analysis demonstrates type V collagen mutations in over 90% of patients with classic EDS and allows to refine diagnostic criteria.

This study suggests a fetal association of COL5A2 and a combined fetal-maternal association of COL5A1 with spontaneous preterm delivery.
Title: Candidate gene analysis of spontaneous preterm delivery: new insights from re-analysis of a case-control study using case-parent triads and control-mother dyads.

The following COL5A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL5A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu102290	NM_000393.5 Latest version!	Homo sapiens collagen type V alpha 2 chain (COL5A2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu56985	XM_011510573.3 Latest version!	Homo sapiens collagen type V alpha 2 chain (COL5A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
View Old Accession Versions >>
OHu102290	NM_000393.4	Homo sapiens collagen type V alpha 2 chain (COL5A2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu56985	XM_011510573.2	Homo sapiens collagen type V alpha 2 (COL5A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL5A2 ( NM_000393.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000384.2 Homo sapiens collagen type V alpha 2 chain (COL5A2), mRNA.

Price & Availability↑

CloneID	OHu102290
Clone ID Related Accession (Same CDS sequence)	NM_000393.4 , NM_000393.5
Accession Version	NM_000393.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	collagen alpha-2(V) chain preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL552427.3, AC133106.3, J04478.1 and AB209045.1. This sequence is a reference standard in the RefSeqGene project. On Nov 30, 2016 this sequence version replaced NM_000393.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.82255.1, AB209045.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGATGGCAA ACTGGGCGGA AGCAAGACCT CTCCTCATTC TTATTGTTTT ATTAGGGCAA 
TTTGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC 
TGCACTCAGA ATGGCCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGTCAG 
ATCTGTGTCT GTGACAATGG AGCCATTCTC TGTGACAAGA TAGAATGCCA GGATGTGCTG 
GACTGTGCCG ACCCTGTAAC GCCCCCTGGG GAATGCTGTC CTGTCTGTTC ACAAACACCT 
GGAGGTGGCA ATACCAATTT TGGTAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA 
GTGCCTGTTG TAACAGGCAT ACGTGGTCGT CCAGGACCGG CAGGACCTCC AGGATCACAG 
GGACCAAGAG GAGAGCGAGG GCCAAAAGGA AGACCTGGCC CTCGTGGACC TCAGGGAATT 
GATGGAGAAC CAGGTGTTCC TGGTCAACCT GGTGCTCCAG GACCTCCTGG ACATCCGTCC 
CACCCAGGAC CCGATGGCTT GAGCAGGCCG TTTTCAGCTC AAATGGCTGG GTTGGATGAA 
AAATCTGGAC TTGGGAGTCA AGTAGGACTA ATGCCTGGCT CTGTGGGTCC TGTTGGCCCA 
AGGGGACCAC AGGGTTTACA AGGACAGCAA GGTGGTGCAG GACCTACAGG ACCTCCTGGT 
GAACCTGGTG ATCCTGGACC AATGGGTCCG ATTGGTTCAC GTGGACCAGA GGGCCCTCCT 
GGTAAACCTG GGGAAGATGG TGAACCTGGC AGAAATGGAA ATCCTGGTGA AGTGGGATTT 
GCAGGATCTC CGGGAGCTCG TGGATTTCCT GGGGCTCCTG GTCTTCCAGG TCTGAAGGGT 
CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCACC TGGTTCCAAG 
GGTGAAGCTG GCCCCACTGG TCCAATGGGT GCCATGGGTC CTCTGGGTCC GAGGGGAATG 
CCAGGAGAGA GAGGGAGACT TGGGCCACAG GGTGCTCCTG GACAACGAGG TGCACATGGT 
ATGCCTGGAA AACCTGGACC AATGGGTCCT CTTGGGATAC CAGGCTCTTC TGGTTTTCCA 
GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCTACAGGGG CGCGAGGCCC TGAAGGTCCT 
CAGGGGCAGA GAGGTGAAAC TGGGCCCCCA GGTCCAGTTG GCTCTCCAGG TCTTCCTGGT 
GCAATAGGAA CTGATGGTAC TCCTGGTGCC AAAGGCCCAA CGGGCTCTCC AGGTACCTCT 
GGTCCTCCTG GCTCAGCAGG GCCTCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGTCCT 
CAGGGAATTC GAGGCCAACC GGGTGATCCA GGAGTTCCAG GTTTCAAAGG AGAAGCTGGC 
CCAAAAGGGG AACCAGGGCC ACATGGTATT CAGGGTCCGA TAGGCCCACC CGGTGAAGAA 
GGCAAAAGAG GTCCCAGAGG TGACCCAGGA ACAGTTGGTC CTCCAGGGCC AGTGGGAGAA 
AGGGGTGCTC CTGGCAATCG TGGTTTTCCA GGCTCTGATG GTTTACCTGG GCCAAAGGGT 
GCTCAAGGAG AACGGGGTCC TGTAGGTTCT TCAGGACCCA AAGGAAGCCA GGGGGATCCA 
GGACGTCCAG GGGAACCTGG GCTTCCAGGT GCTCGGGGTT TGACAGGAAA TCCTGGTGTT 
CAAGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCGCCAG GGGAAGATGG CCGTCCAGGT 
CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCCGGGAGCA TGGGCCTTCC AGGCCCCAAA 
GGTAGCAGTG GTGACCCTGG GAAACCTGGA GAAGCAGGAA ATGCTGGAGT TCCTGGGCAG 
AGGGGAGCTC CTGGAAAAGA TGGTGAAGTT GGTCCTTCTG GTCCTGTGGG CCCGCCGGGT 
CTAGCTGGTG AAAGAGGAGA ACAAGGACCT CCAGGCCCCA CAGGTTTTCA GGGGCTTCCT 
GGTCCTCCAG GGCCTCCTGG AGAAGGTGGA AAACCAGGTG ATCAAGGTGT TCCTGGAGAT 
CCCGGAGCAG TTGGCCCGTT AGGACCTAGA GGAGAACGAG GAAATCCTGG GGAAAGAGGA 
GAACCTGGGA TAACTGGACT CCCTGGTGAG AAGGGAATGG CTGGAGGACA TGGTCCTGAT 
GGCCCAAAAG GCAGTCCAGG TCCATCTGGG ACCCCTGGAG ATACAGGCCC ACCAGGTCTT 
CAAGGTATGC CGGGAGAAAG AGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT 
GGCATAGGAG AAAAAGGTGC TGAAGGCACA GCTGGAAATG ATGGTGCAAG AGGTCTTCCA 
GGTCCTTTGG GCCCTCCAGG TCCGGCAGGT CCTACTGGAG AAAAGGGTGA ACCTGGTCCT 
CGAGGTTTAG TTGGCCCTCC TGGCTCCCGG GGCAATCCTG GTTCTCGAGG TGAAAATGGG 
CCAACTGGAG CTGTTGGTTT TGCCGGACCC CAGGGTCCTG ACGGACAGCC TGGAGTAAAA 
GGTGAACCTG GAGAGCCAGG ACAGAAGGGA GATGCTGGTT CTCCTGGACC ACAAGGTTTA 
GCAGGATCCC CTGGCCCTCA TGGTCCTAAT GGTGTTCCTG GACTAAAAGG TGGTCGAGGA 
ACCCAAGGTC CGCCTGGTGC TACAGGATTT CCTGGTTCTG CGGGCAGAGT TGGACCTCCA 
GGCCCTGCTG GAGCTCCAGG ACCTGCGGGA CCCCTAGGGG AACCCGGGAA GGAGGGACCT 
CCAGGTCTTC GTGGGGACCC TGGCTCTCAT GGGCGTGTGG GAGATCGAGG ACCAGCTGGC 
CCCCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGGCAACC TGGTCCAGAT 
GGCCCCCCTG GTCCAGCTGG AACGACCGGG CAGAGAGGAA TTGTTGGCAT GCCTGGGCAA 
CGTGGAGAGA GAGGCATGCC CGGCCTACCA GGCCCAGCGG GAACACCAGG AAAAGTAGGA 
CCAACTGGTG CAACAGGAGA TAAAGGTCCA CCTGGACCTG TGGGGCCCCC AGGCTCCAAT 
GGTCCTGTAG GGGAACCTGG ACCAGAAGGT CCAGCTGGCA ATGATGGTAC CCCAGGACGG 
GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC 
TCTCAGGGTG CCCCTGGAAC TCCTGGCCCT GTGGGTGCTC CAGGAGATGC AGGACAAAGA 
GGAGATCCGG GTTCTCGGGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGATTA 
CCTGGACCCC AAGGACCTCG TGGTGACAAA GGTGATCATG GAGACCGAGG TGACAGAGGT 
CAGAAGGGCC ACAGAGGCTT TACTGGTCTT CAGGGTCTTC CTGGCCCTCC TGGTCCAAAT 
GGTGAACAAG GAAGTGCTGG AATCCCTGGA CCATTTGGCC CAAGAGGTCC TCCAGGCCCA 
GTTGGTCCTT CAGGTAAAGA AGGAAACCCT GGGCCACTTG GGCCAATTGG ACCTCCAGGT 
GTACGAGGCA GTGTAGGAGA AGCAGGACCT GAGGGCCCTC CTGGTGAGCC TGGCCCACCT 
GGCCCTCCGG GTCCCCCTGG CCACCTTACA GCTGCTCTTG GGGATATCAT GGGGCACTAT 
GATGAAAGCA TGCCAGATCC ACTTCCTGAG TTTACTGAAG ATCAGGCGGC TCCTGATGAC 
AAAAACAAAA CGGACCCAGG GGTTCATGCT ACCCTGAAGT CACTCAGTAG TCAGATTGAA 
ACCATGCGCA GCCCCGATGG CTCGAAAAAG CACCCAGCCC GCACGTGTGA TGACCTAAAG 
CTTTGCCATT CCGCAAAGCA GAGTGGTGAA TACTGGATTG ATCCTAACCA AGGATCTGTT 
GAAGATGCAA TCAAAGTTTA CTGCAACATG GAAACAGGAG AAACATGTAT TTCAGCAAAC 
CCATCCAGTG TACCACGTAA AACCTGGTGG GCCAGTAAAT CTCCTGACAA TAAACCTGTT 
TGGTATGGTC TTGATATGAA CAGAGGGTCT CAGTTCGCTT ATGGAGACCA CCAATCACCT 
AATACAGCCA TTACTCAGAT GACTTTTTTG CGCCTTTTAT CAAAAGAAGC CTCCCAGAAC 
ATCACTTACA TCTGTAAAAA CAGTGTAGGA TACATGGACG ATCAAGCTAA GAACCTCAAA 
AAAGCTGTGG TTCTCAAAGG GGCAAATGAC TTAGATATCA AAGCAGAGGG AAATATTAGA 
TTCCGGTATA TCGTTCTTCA AGACACTTGC TCTAAGCGGA ATGGAAATGT GGGCAAGACT 
GTCTTTGAAT ATAGAACACA GAATGTGGCA CGCTTGCCCA TCATAGATCT TGCTCCTGTG 
GATGTTGGCG GCACAGACCA GGAATTCGGC GTTGAAATTG GGCCAGTTTG TTTTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000384.2
CDS	276..4775
Translation	MMANWAEARPLLILIVLLGQFVSIKAQEEDEDEGYGEEIACTQNGQMYLNRDIWKPAPCQICVCDNGAILCDKIECQDVLDCADPVTPPGECCPVCSQTPGGGNTNFGRGRKGQKGEPGLVPVVTGIRGRPGPAGPPGSQGPRGERGPKGRPGPRGPQGIDGEPGVPGQPGAPGPPGHPSHPGPDGLSRPFSAQMAGLDEKSGLGSQVGLMPGSVGPVGPRGPQGLQGQQGGAGPTGPPGEPGDPGPMGPIGSRGPEGPPGKPGEDGEPGRNGNPGEVGFAGSPGARGFPGAPGLPGLKGHRGHKGLEGPKGEVGAPGSKGEAGPTGPMGAMGPLGPRGMPGERGRLGPQGAPGQRGAHGMPGKPGPMGPLGIPGSSGFPGNPGMKGEAGPTGARGPEGPQGQRGETGPPGPVGSPGLPGAIGTDGTPGAKGPTGSPGTSGPPGSAGPPGSPGPQGSTGPQGIRGQPGDPGVPGFKGEAGPKGEPGPHGIQGPIGPPGEEGKRGPRGDPGTVGPPGPVGERGAPGNRGFPGSDGLPGPKGAQGERGPVGSSGPKGSQGDPGRPGEPGLPGARGLTGNPGVQGPEGKLGPLGAPGEDGRPGPPGSIGIRGQPGSMGLPGPKGSSGDPGKPGEAGNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEGGKPGDQGVPGDPGAVGPLGPRGERGNPGERGEPGITGLPGEKGMAGGHGPDGPKGSPGPSGTPGDTGPPGLQGMPGERGIAGTPGPKGDRGGIGEKGAEGTAGNDGARGLPGPLGPPGPAGPTGEKGEPGPRGLVGPPGSRGNPGSRGENGPTGAVGFAGPQGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPHGPNGVPGLKGGRGTQGPPGATGFPGSAGRVGPPGPAGAPGPAGPLGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGGPGDKGDPGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKVGPTGATGDKGPPGPVGPPGSNGPVGEPGPEGPAGNDGTPGRDGAVGERGDRGDPGPAGLPGSQGAPGTPGPVGAPGDAGQRGDPGSRGPIGPPGRAGKRGLPGPQGPRGDKGDHGDRGDRGQKGHRGFTGLQGLPGPPGPNGEQGSAGIPGPFGPRGPPGPVGPSGKEGNPGPLGPIGPPGVRGSVGEAGPEGPPGEPGPPGPPGPPGHLTAALGDIMGHYDESMPDPLPEFTEDQAAPDDKNKTDPGVHATLKSLSSQIETMRSPDGSKKHPARTCDDLKLCHSAKQSGEYWIDPNQGSVEDAIKVYCNMETGETCISANPSSVPRKTWWASKSPDNKPVWYGLDMNRGSQFAYGDHQSPNTAITQMTFLRLLSKEASQNITYICKNSVGYMDDQAKNLKKAVVLKGANDLDIKAEGNIRFRYIVLQDTCSKRNGNVGKTVFEYRTQNVARLPIIDLAPVDVGGTDQEFGVEIGPVCFV

Target ORF information:

RefSeq Version	NM_000393.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type V alpha 2 chain (COL5A2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000393.4

ORF Insert Sequence:

ATGATGGCAA ACTGGGCGGA AGCAAGACCT CTCCTCATTC TTATTGTTTT ATTAGGGCAA 
TTTGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC 
TGCACTCAGA ATGGCCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGTCAG 
ATCTGTGTCT GTGACAATGG AGCCATTCTC TGTGACAAGA TAGAATGCCA GGATGTGCTG 
GACTGTGCCG ACCCTGTAAC GCCCCCTGGG GAATGCTGTC CTGTCTGTTC ACAAACACCT 
GGAGGTGGCA ATACCAATTT TGGTAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA 
GTGCCTGTTG TAACAGGCAT ACGTGGTCGT CCAGGACCGG CAGGACCTCC AGGATCACAG 
GGACCAAGAG GAGAGCGAGG GCCAAAAGGA AGACCTGGCC CTCGTGGACC TCAGGGAATT 
GATGGAGAAC CAGGTGTTCC TGGTCAACCT GGTGCTCCAG GACCTCCTGG ACATCCGTCC 
CACCCAGGAC CCGATGGCTT GAGCAGGCCG TTTTCAGCTC AAATGGCTGG GTTGGATGAA 
AAATCTGGAC TTGGGAGTCA AGTAGGACTA ATGCCTGGCT CTGTGGGTCC TGTTGGCCCA 
AGGGGACCAC AGGGTTTACA AGGACAGCAA GGTGGTGCAG GACCTACAGG ACCTCCTGGT 
GAACCTGGTG ATCCTGGACC AATGGGTCCG ATTGGTTCAC GTGGACCAGA GGGCCCTCCT 
GGTAAACCTG GGGAAGATGG TGAACCTGGC AGAAATGGAA ATCCTGGTGA AGTGGGATTT 
GCAGGATCTC CGGGAGCTCG TGGATTTCCT GGGGCTCCTG GTCTTCCAGG TCTGAAGGGT 
CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCACC TGGTTCCAAG 
GGTGAAGCTG GCCCCACTGG TCCAATGGGT GCCATGGGTC CTCTGGGTCC GAGGGGAATG 
CCAGGAGAGA GAGGGAGACT TGGGCCACAG GGTGCTCCTG GACAACGAGG TGCACATGGT 
ATGCCTGGAA AACCTGGACC AATGGGTCCT CTTGGGATAC CAGGCTCTTC TGGTTTTCCA 
GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCTACAGGGG CGCGAGGCCC TGAAGGTCCT 
CAGGGGCAGA GAGGTGAAAC TGGGCCCCCA GGTCCAGTTG GCTCTCCAGG TCTTCCTGGT 
GCAATAGGAA CTGATGGTAC TCCTGGTGCC AAAGGCCCAA CGGGCTCTCC AGGTACCTCT 
GGTCCTCCTG GCTCAGCAGG GCCTCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGTCCT 
CAGGGAATTC GAGGCCAACC GGGTGATCCA GGAGTTCCAG GTTTCAAAGG AGAAGCTGGC 
CCAAAAGGGG AACCAGGGCC ACATGGTATT CAGGGTCCGA TAGGCCCACC CGGTGAAGAA 
GGCAAAAGAG GTCCCAGAGG TGACCCAGGA ACAGTTGGTC CTCCAGGGCC AGTGGGAGAA 
AGGGGTGCTC CTGGCAATCG TGGTTTTCCA GGCTCTGATG GTTTACCTGG GCCAAAGGGT 
GCTCAAGGAG AACGGGGTCC TGTAGGTTCT TCAGGACCCA AAGGAAGCCA GGGGGATCCA 
GGACGTCCAG GGGAACCTGG GCTTCCAGGT GCTCGGGGTT TGACAGGAAA TCCTGGTGTT 
CAAGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCGCCAG GGGAAGATGG CCGTCCAGGT 
CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCCGGGAGCA TGGGCCTTCC AGGCCCCAAA 
GGTAGCAGTG GTGACCCTGG GAAACCTGGA GAAGCAGGAA ATGCTGGAGT TCCTGGGCAG 
AGGGGAGCTC CTGGAAAAGA TGGTGAAGTT GGTCCTTCTG GTCCTGTGGG CCCGCCGGGT 
CTAGCTGGTG AAAGAGGAGA ACAAGGACCT CCAGGCCCCA CAGGTTTTCA GGGGCTTCCT 
GGTCCTCCAG GGCCTCCTGG AGAAGGTGGA AAACCAGGTG ATCAAGGTGT TCCTGGAGAT 
CCCGGAGCAG TTGGCCCGTT AGGACCTAGA GGAGAACGAG GAAATCCTGG GGAAAGAGGA 
GAACCTGGGA TAACTGGACT CCCTGGTGAG AAGGGAATGG CTGGAGGACA TGGTCCTGAT 
GGCCCAAAAG GCAGTCCAGG TCCATCTGGG ACCCCTGGAG ATACAGGCCC ACCAGGTCTT 
CAAGGTATGC CGGGAGAAAG AGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT 
GGCATAGGAG AAAAAGGTGC TGAAGGCACA GCTGGAAATG ATGGTGCAAG AGGTCTTCCA 
GGTCCTTTGG GCCCTCCAGG TCCGGCAGGT CCTACTGGAG AAAAGGGTGA ACCTGGTCCT 
CGAGGTTTAG TTGGCCCTCC TGGCTCCCGG GGCAATCCTG GTTCTCGAGG TGAAAATGGG 
CCAACTGGAG CTGTTGGTTT TGCCGGACCC CAGGGTCCTG ACGGACAGCC TGGAGTAAAA 
GGTGAACCTG GAGAGCCAGG ACAGAAGGGA GATGCTGGTT CTCCTGGACC ACAAGGTTTA 
GCAGGATCCC CTGGCCCTCA TGGTCCTAAT GGTGTTCCTG GACTAAAAGG TGGTCGAGGA 
ACCCAAGGTC CGCCTGGTGC TACAGGATTT CCTGGTTCTG CGGGCAGAGT TGGACCTCCA 
GGCCCTGCTG GAGCTCCAGG ACCTGCGGGA CCCCTAGGGG AACCCGGGAA GGAGGGACCT 
CCAGGTCTTC GTGGGGACCC TGGCTCTCAT GGGCGTGTGG GAGATCGAGG ACCAGCTGGC 
CCCCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGGCAACC TGGTCCAGAT 
GGCCCCCCTG GTCCAGCTGG AACGACCGGG CAGAGAGGAA TTGTTGGCAT GCCTGGGCAA 
CGTGGAGAGA GAGGCATGCC CGGCCTACCA GGCCCAGCGG GAACACCAGG AAAAGTAGGA 
CCAACTGGTG CAACAGGAGA TAAAGGTCCA CCTGGACCTG TGGGGCCCCC AGGCTCCAAT 
GGTCCTGTAG GGGAACCTGG ACCAGAAGGT CCAGCTGGCA ATGATGGTAC CCCAGGACGG 
GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC 
TCTCAGGGTG CCCCTGGAAC TCCTGGCCCT GTGGGTGCTC CAGGAGATGC AGGACAAAGA 
GGAGATCCGG GTTCTCGGGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGATTA 
CCTGGACCCC AAGGACCTCG TGGTGACAAA GGTGATCATG GAGACCGAGG TGACAGAGGT 
CAGAAGGGCC ACAGAGGCTT TACTGGTCTT CAGGGTCTTC CTGGCCCTCC TGGTCCAAAT 
GGTGAACAAG GAAGTGCTGG AATCCCTGGA CCATTTGGCC CAAGAGGTCC TCCAGGCCCA 
GTTGGTCCTT CAGGTAAAGA AGGAAACCCT GGGCCACTTG GGCCAATTGG ACCTCCAGGT 
GTACGAGGCA GTGTAGGAGA AGCAGGACCT GAGGGCCCTC CTGGTGAGCC TGGCCCACCT 
GGCCCTCCGG GTCCCCCTGG CCACCTTACA GCTGCTCTTG GGGATATCAT GGGGCACTAT 
GATGAAAGCA TGCCAGATCC ACTTCCTGAG TTTACTGAAG ATCAGGCGGC TCCTGATGAC 
AAAAACAAAA CGGACCCAGG GGTTCATGCT ACCCTGAAGT CACTCAGTAG TCAGATTGAA 
ACCATGCGCA GCCCCGATGG CTCGAAAAAG CACCCAGCCC GCACGTGTGA TGACCTAAAG 
CTTTGCCATT CCGCAAAGCA GAGTGGTGAA TACTGGATTG ATCCTAACCA AGGATCTGTT 
GAAGATGCAA TCAAAGTTTA CTGCAACATG GAAACAGGAG AAACATGTAT TTCAGCAAAC 
CCATCCAGTG TACCACGTAA AACCTGGTGG GCCAGTAAAT CTCCTGACAA TAAACCTGTT 
TGGTATGGTC TTGATATGAA CAGAGGGTCT CAGTTCGCTT ATGGAGACCA CCAATCACCT 
AATACAGCCA TTACTCAGAT GACTTTTTTG CGCCTTTTAT CAAAAGAAGC CTCCCAGAAC 
ATCACTTACA TCTGTAAAAA CAGTGTAGGA TACATGGACG ATCAAGCTAA GAACCTCAAA 
AAAGCTGTGG TTCTCAAAGG GGCAAATGAC TTAGATATCA AAGCAGAGGG AAATATTAGA 
TTCCGGTATA TCGTTCTTCA AGACACTTGC TCTAAGCGGA ATGGAAATGT GGGCAAGACT 
GTCTTTGAAT ATAGAACACA GAATGTGGCA CGCTTGCCCA TCATAGATCT TGCTCCTGTG 
GATGTTGGCG GCACAGACCA GGAATTCGGC GTTGAAATTG GGCCAGTTTG TTTTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A2 ( NM_000393.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000384.2 Homo sapiens collagen type V alpha 2 chain (COL5A2), mRNA.

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CloneID	OHu102290
Clone ID Related Accession (Same CDS sequence)	NM_000393.4 , NM_000393.5
Accession Version	NM_000393.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	collagen alpha-2(V) chain preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL552427.3, AC133106.3, J04478.1 and AB209045.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000393.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.82255.1, AB209045.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000374866.9/ ENSP00000364000.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATGGCAA ACTGGGCGGA AGCAAGACCT CTCCTCATTC TTATTGTTTT ATTAGGGCAA 
TTTGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC 
TGCACTCAGA ATGGCCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGTCAG 
ATCTGTGTCT GTGACAATGG AGCCATTCTC TGTGACAAGA TAGAATGCCA GGATGTGCTG 
GACTGTGCCG ACCCTGTAAC GCCCCCTGGG GAATGCTGTC CTGTCTGTTC ACAAACACCT 
GGAGGTGGCA ATACCAATTT TGGTAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA 
GTGCCTGTTG TAACAGGCAT ACGTGGTCGT CCAGGACCGG CAGGACCTCC AGGATCACAG 
GGACCAAGAG GAGAGCGAGG GCCAAAAGGA AGACCTGGCC CTCGTGGACC TCAGGGAATT 
GATGGAGAAC CAGGTGTTCC TGGTCAACCT GGTGCTCCAG GACCTCCTGG ACATCCGTCC 
CACCCAGGAC CCGATGGCTT GAGCAGGCCG TTTTCAGCTC AAATGGCTGG GTTGGATGAA 
AAATCTGGAC TTGGGAGTCA AGTAGGACTA ATGCCTGGCT CTGTGGGTCC TGTTGGCCCA 
AGGGGACCAC AGGGTTTACA AGGACAGCAA GGTGGTGCAG GACCTACAGG ACCTCCTGGT 
GAACCTGGTG ATCCTGGACC AATGGGTCCG ATTGGTTCAC GTGGACCAGA GGGCCCTCCT 
GGTAAACCTG GGGAAGATGG TGAACCTGGC AGAAATGGAA ATCCTGGTGA AGTGGGATTT 
GCAGGATCTC CGGGAGCTCG TGGATTTCCT GGGGCTCCTG GTCTTCCAGG TCTGAAGGGT 
CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCACC TGGTTCCAAG 
GGTGAAGCTG GCCCCACTGG TCCAATGGGT GCCATGGGTC CTCTGGGTCC GAGGGGAATG 
CCAGGAGAGA GAGGGAGACT TGGGCCACAG GGTGCTCCTG GACAACGAGG TGCACATGGT 
ATGCCTGGAA AACCTGGACC AATGGGTCCT CTTGGGATAC CAGGCTCTTC TGGTTTTCCA 
GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCTACAGGGG CGCGAGGCCC TGAAGGTCCT 
CAGGGGCAGA GAGGTGAAAC TGGGCCCCCA GGTCCAGTTG GCTCTCCAGG TCTTCCTGGT 
GCAATAGGAA CTGATGGTAC TCCTGGTGCC AAAGGCCCAA CGGGCTCTCC AGGTACCTCT 
GGTCCTCCTG GCTCAGCAGG GCCTCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGTCCT 
CAGGGAATTC GAGGCCAACC GGGTGATCCA GGAGTTCCAG GTTTCAAAGG AGAAGCTGGC 
CCAAAAGGGG AACCAGGGCC ACATGGTATT CAGGGTCCGA TAGGCCCACC CGGTGAAGAA 
GGCAAAAGAG GTCCCAGAGG TGACCCAGGA ACAGTTGGTC CTCCAGGGCC AGTGGGAGAA 
AGGGGTGCTC CTGGCAATCG TGGTTTTCCA GGCTCTGATG GTTTACCTGG GCCAAAGGGT 
GCTCAAGGAG AACGGGGTCC TGTAGGTTCT TCAGGACCCA AAGGAAGCCA GGGGGATCCA 
GGACGTCCAG GGGAACCTGG GCTTCCAGGT GCTCGGGGTT TGACAGGAAA TCCTGGTGTT 
CAAGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCGCCAG GGGAAGATGG CCGTCCAGGT 
CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCCGGGAGCA TGGGCCTTCC AGGCCCCAAA 
GGTAGCAGTG GTGACCCTGG GAAACCTGGA GAAGCAGGAA ATGCTGGAGT TCCTGGGCAG 
AGGGGAGCTC CTGGAAAAGA TGGTGAAGTT GGTCCTTCTG GTCCTGTGGG CCCGCCGGGT 
CTAGCTGGTG AAAGAGGAGA ACAAGGACCT CCAGGCCCCA CAGGTTTTCA GGGGCTTCCT 
GGTCCTCCAG GGCCTCCTGG AGAAGGTGGA AAACCAGGTG ATCAAGGTGT TCCTGGAGAT 
CCCGGAGCAG TTGGCCCGTT AGGACCTAGA GGAGAACGAG GAAATCCTGG GGAAAGAGGA 
GAACCTGGGA TAACTGGACT CCCTGGTGAG AAGGGAATGG CTGGAGGACA TGGTCCTGAT 
GGCCCAAAAG GCAGTCCAGG TCCATCTGGG ACCCCTGGAG ATACAGGCCC ACCAGGTCTT 
CAAGGTATGC CGGGAGAAAG AGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT 
GGCATAGGAG AAAAAGGTGC TGAAGGCACA GCTGGAAATG ATGGTGCAAG AGGTCTTCCA 
GGTCCTTTGG GCCCTCCAGG TCCGGCAGGT CCTACTGGAG AAAAGGGTGA ACCTGGTCCT 
CGAGGTTTAG TTGGCCCTCC TGGCTCCCGG GGCAATCCTG GTTCTCGAGG TGAAAATGGG 
CCAACTGGAG CTGTTGGTTT TGCCGGACCC CAGGGTCCTG ACGGACAGCC TGGAGTAAAA 
GGTGAACCTG GAGAGCCAGG ACAGAAGGGA GATGCTGGTT CTCCTGGACC ACAAGGTTTA 
GCAGGATCCC CTGGCCCTCA TGGTCCTAAT GGTGTTCCTG GACTAAAAGG TGGTCGAGGA 
ACCCAAGGTC CGCCTGGTGC TACAGGATTT CCTGGTTCTG CGGGCAGAGT TGGACCTCCA 
GGCCCTGCTG GAGCTCCAGG ACCTGCGGGA CCCCTAGGGG AACCCGGGAA GGAGGGACCT 
CCAGGTCTTC GTGGGGACCC TGGCTCTCAT GGGCGTGTGG GAGATCGAGG ACCAGCTGGC 
CCCCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGGCAACC TGGTCCAGAT 
GGCCCCCCTG GTCCAGCTGG AACGACCGGG CAGAGAGGAA TTGTTGGCAT GCCTGGGCAA 
CGTGGAGAGA GAGGCATGCC CGGCCTACCA GGCCCAGCGG GAACACCAGG AAAAGTAGGA 
CCAACTGGTG CAACAGGAGA TAAAGGTCCA CCTGGACCTG TGGGGCCCCC AGGCTCCAAT 
GGTCCTGTAG GGGAACCTGG ACCAGAAGGT CCAGCTGGCA ATGATGGTAC CCCAGGACGG 
GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC 
TCTCAGGGTG CCCCTGGAAC TCCTGGCCCT GTGGGTGCTC CAGGAGATGC AGGACAAAGA 
GGAGATCCGG GTTCTCGGGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGATTA 
CCTGGACCCC AAGGACCTCG TGGTGACAAA GGTGATCATG GAGACCGAGG TGACAGAGGT 
CAGAAGGGCC ACAGAGGCTT TACTGGTCTT CAGGGTCTTC CTGGCCCTCC TGGTCCAAAT 
GGTGAACAAG GAAGTGCTGG AATCCCTGGA CCATTTGGCC CAAGAGGTCC TCCAGGCCCA 
GTTGGTCCTT CAGGTAAAGA AGGAAACCCT GGGCCACTTG GGCCAATTGG ACCTCCAGGT 
GTACGAGGCA GTGTAGGAGA AGCAGGACCT GAGGGCCCTC CTGGTGAGCC TGGCCCACCT 
GGCCCTCCGG GTCCCCCTGG CCACCTTACA GCTGCTCTTG GGGATATCAT GGGGCACTAT 
GATGAAAGCA TGCCAGATCC ACTTCCTGAG TTTACTGAAG ATCAGGCGGC TCCTGATGAC 
AAAAACAAAA CGGACCCAGG GGTTCATGCT ACCCTGAAGT CACTCAGTAG TCAGATTGAA 
ACCATGCGCA GCCCCGATGG CTCGAAAAAG CACCCAGCCC GCACGTGTGA TGACCTAAAG 
CTTTGCCATT CCGCAAAGCA GAGTGGTGAA TACTGGATTG ATCCTAACCA AGGATCTGTT 
GAAGATGCAA TCAAAGTTTA CTGCAACATG GAAACAGGAG AAACATGTAT TTCAGCAAAC 
CCATCCAGTG TACCACGTAA AACCTGGTGG GCCAGTAAAT CTCCTGACAA TAAACCTGTT 
TGGTATGGTC TTGATATGAA CAGAGGGTCT CAGTTCGCTT ATGGAGACCA CCAATCACCT 
AATACAGCCA TTACTCAGAT GACTTTTTTG CGCCTTTTAT CAAAAGAAGC CTCCCAGAAC 
ATCACTTACA TCTGTAAAAA CAGTGTAGGA TACATGGACG ATCAAGCTAA GAACCTCAAA 
AAAGCTGTGG TTCTCAAAGG GGCAAATGAC TTAGATATCA AAGCAGAGGG AAATATTAGA 
TTCCGGTATA TCGTTCTTCA AGACACTTGC TCTAAGCGGA ATGGAAATGT GGGCAAGACT 
GTCTTTGAAT ATAGAACACA GAATGTGGCA CGCTTGCCCA TCATAGATCT TGCTCCTGTG 
GATGTTGGCG GCACAGACCA GGAATTCGGC GTTGAAATTG GGCCAGTTTG TTTTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000384.2
CDS	158..4657
Translation	MMANWAEARPLLILIVLLGQFVSIKAQEEDEDEGYGEEIACTQNGQMYLNRDIWKPAPCQICVCDNGAILCDKIECQDVLDCADPVTPPGECCPVCSQTPGGGNTNFGRGRKGQKGEPGLVPVVTGIRGRPGPAGPPGSQGPRGERGPKGRPGPRGPQGIDGEPGVPGQPGAPGPPGHPSHPGPDGLSRPFSAQMAGLDEKSGLGSQVGLMPGSVGPVGPRGPQGLQGQQGGAGPTGPPGEPGDPGPMGPIGSRGPEGPPGKPGEDGEPGRNGNPGEVGFAGSPGARGFPGAPGLPGLKGHRGHKGLEGPKGEVGAPGSKGEAGPTGPMGAMGPLGPRGMPGERGRLGPQGAPGQRGAHGMPGKPGPMGPLGIPGSSGFPGNPGMKGEAGPTGARGPEGPQGQRGETGPPGPVGSPGLPGAIGTDGTPGAKGPTGSPGTSGPPGSAGPPGSPGPQGSTGPQGIRGQPGDPGVPGFKGEAGPKGEPGPHGIQGPIGPPGEEGKRGPRGDPGTVGPPGPVGERGAPGNRGFPGSDGLPGPKGAQGERGPVGSSGPKGSQGDPGRPGEPGLPGARGLTGNPGVQGPEGKLGPLGAPGEDGRPGPPGSIGIRGQPGSMGLPGPKGSSGDPGKPGEAGNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEGGKPGDQGVPGDPGAVGPLGPRGERGNPGERGEPGITGLPGEKGMAGGHGPDGPKGSPGPSGTPGDTGPPGLQGMPGERGIAGTPGPKGDRGGIGEKGAEGTAGNDGARGLPGPLGPPGPAGPTGEKGEPGPRGLVGPPGSRGNPGSRGENGPTGAVGFAGPQGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPHGPNGVPGLKGGRGTQGPPGATGFPGSAGRVGPPGPAGAPGPAGPLGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGGPGDKGDPGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKVGPTGATGDKGPPGPVGPPGSNGPVGEPGPEGPAGNDGTPGRDGAVGERGDRGDPGPAGLPGSQGAPGTPGPVGAPGDAGQRGDPGSRGPIGPPGRAGKRGLPGPQGPRGDKGDHGDRGDRGQKGHRGFTGLQGLPGPPGPNGEQGSAGIPGPFGPRGPPGPVGPSGKEGNPGPLGPIGPPGVRGSVGEAGPEGPPGEPGPPGPPGPPGHLTAALGDIMGHYDESMPDPLPEFTEDQAAPDDKNKTDPGVHATLKSLSSQIETMRSPDGSKKHPARTCDDLKLCHSAKQSGEYWIDPNQGSVEDAIKVYCNMETGETCISANPSSVPRKTWWASKSPDNKPVWYGLDMNRGSQFAYGDHQSPNTAITQMTFLRLLSKEASQNITYICKNSVGYMDDQAKNLKKAVVLKGANDLDIKAEGNIRFRYIVLQDTCSKRNGNVGKTVFEYRTQNVARLPIIDLAPVDVGGTDQEFGVEIGPVCFV

Target ORF information:

RefSeq Version	NM_000393.5
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type V alpha 2 chain (COL5A2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000393.5

ORF Insert Sequence:

ATGATGGCAA ACTGGGCGGA AGCAAGACCT CTCCTCATTC TTATTGTTTT ATTAGGGCAA 
TTTGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC 
TGCACTCAGA ATGGCCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGTCAG 
ATCTGTGTCT GTGACAATGG AGCCATTCTC TGTGACAAGA TAGAATGCCA GGATGTGCTG 
GACTGTGCCG ACCCTGTAAC GCCCCCTGGG GAATGCTGTC CTGTCTGTTC ACAAACACCT 
GGAGGTGGCA ATACCAATTT TGGTAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA 
GTGCCTGTTG TAACAGGCAT ACGTGGTCGT CCAGGACCGG CAGGACCTCC AGGATCACAG 
GGACCAAGAG GAGAGCGAGG GCCAAAAGGA AGACCTGGCC CTCGTGGACC TCAGGGAATT 
GATGGAGAAC CAGGTGTTCC TGGTCAACCT GGTGCTCCAG GACCTCCTGG ACATCCGTCC 
CACCCAGGAC CCGATGGCTT GAGCAGGCCG TTTTCAGCTC AAATGGCTGG GTTGGATGAA 
AAATCTGGAC TTGGGAGTCA AGTAGGACTA ATGCCTGGCT CTGTGGGTCC TGTTGGCCCA 
AGGGGACCAC AGGGTTTACA AGGACAGCAA GGTGGTGCAG GACCTACAGG ACCTCCTGGT 
GAACCTGGTG ATCCTGGACC AATGGGTCCG ATTGGTTCAC GTGGACCAGA GGGCCCTCCT 
GGTAAACCTG GGGAAGATGG TGAACCTGGC AGAAATGGAA ATCCTGGTGA AGTGGGATTT 
GCAGGATCTC CGGGAGCTCG TGGATTTCCT GGGGCTCCTG GTCTTCCAGG TCTGAAGGGT 
CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCACC TGGTTCCAAG 
GGTGAAGCTG GCCCCACTGG TCCAATGGGT GCCATGGGTC CTCTGGGTCC GAGGGGAATG 
CCAGGAGAGA GAGGGAGACT TGGGCCACAG GGTGCTCCTG GACAACGAGG TGCACATGGT 
ATGCCTGGAA AACCTGGACC AATGGGTCCT CTTGGGATAC CAGGCTCTTC TGGTTTTCCA 
GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCTACAGGGG CGCGAGGCCC TGAAGGTCCT 
CAGGGGCAGA GAGGTGAAAC TGGGCCCCCA GGTCCAGTTG GCTCTCCAGG TCTTCCTGGT 
GCAATAGGAA CTGATGGTAC TCCTGGTGCC AAAGGCCCAA CGGGCTCTCC AGGTACCTCT 
GGTCCTCCTG GCTCAGCAGG GCCTCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGTCCT 
CAGGGAATTC GAGGCCAACC GGGTGATCCA GGAGTTCCAG GTTTCAAAGG AGAAGCTGGC 
CCAAAAGGGG AACCAGGGCC ACATGGTATT CAGGGTCCGA TAGGCCCACC CGGTGAAGAA 
GGCAAAAGAG GTCCCAGAGG TGACCCAGGA ACAGTTGGTC CTCCAGGGCC AGTGGGAGAA 
AGGGGTGCTC CTGGCAATCG TGGTTTTCCA GGCTCTGATG GTTTACCTGG GCCAAAGGGT 
GCTCAAGGAG AACGGGGTCC TGTAGGTTCT TCAGGACCCA AAGGAAGCCA GGGGGATCCA 
GGACGTCCAG GGGAACCTGG GCTTCCAGGT GCTCGGGGTT TGACAGGAAA TCCTGGTGTT 
CAAGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCGCCAG GGGAAGATGG CCGTCCAGGT 
CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCCGGGAGCA TGGGCCTTCC AGGCCCCAAA 
GGTAGCAGTG GTGACCCTGG GAAACCTGGA GAAGCAGGAA ATGCTGGAGT TCCTGGGCAG 
AGGGGAGCTC CTGGAAAAGA TGGTGAAGTT GGTCCTTCTG GTCCTGTGGG CCCGCCGGGT 
CTAGCTGGTG AAAGAGGAGA ACAAGGACCT CCAGGCCCCA CAGGTTTTCA GGGGCTTCCT 
GGTCCTCCAG GGCCTCCTGG AGAAGGTGGA AAACCAGGTG ATCAAGGTGT TCCTGGAGAT 
CCCGGAGCAG TTGGCCCGTT AGGACCTAGA GGAGAACGAG GAAATCCTGG GGAAAGAGGA 
GAACCTGGGA TAACTGGACT CCCTGGTGAG AAGGGAATGG CTGGAGGACA TGGTCCTGAT 
GGCCCAAAAG GCAGTCCAGG TCCATCTGGG ACCCCTGGAG ATACAGGCCC ACCAGGTCTT 
CAAGGTATGC CGGGAGAAAG AGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT 
GGCATAGGAG AAAAAGGTGC TGAAGGCACA GCTGGAAATG ATGGTGCAAG AGGTCTTCCA 
GGTCCTTTGG GCCCTCCAGG TCCGGCAGGT CCTACTGGAG AAAAGGGTGA ACCTGGTCCT 
CGAGGTTTAG TTGGCCCTCC TGGCTCCCGG GGCAATCCTG GTTCTCGAGG TGAAAATGGG 
CCAACTGGAG CTGTTGGTTT TGCCGGACCC CAGGGTCCTG ACGGACAGCC TGGAGTAAAA 
GGTGAACCTG GAGAGCCAGG ACAGAAGGGA GATGCTGGTT CTCCTGGACC ACAAGGTTTA 
GCAGGATCCC CTGGCCCTCA TGGTCCTAAT GGTGTTCCTG GACTAAAAGG TGGTCGAGGA 
ACCCAAGGTC CGCCTGGTGC TACAGGATTT CCTGGTTCTG CGGGCAGAGT TGGACCTCCA 
GGCCCTGCTG GAGCTCCAGG ACCTGCGGGA CCCCTAGGGG AACCCGGGAA GGAGGGACCT 
CCAGGTCTTC GTGGGGACCC TGGCTCTCAT GGGCGTGTGG GAGATCGAGG ACCAGCTGGC 
CCCCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGGCAACC TGGTCCAGAT 
GGCCCCCCTG GTCCAGCTGG AACGACCGGG CAGAGAGGAA TTGTTGGCAT GCCTGGGCAA 
CGTGGAGAGA GAGGCATGCC CGGCCTACCA GGCCCAGCGG GAACACCAGG AAAAGTAGGA 
CCAACTGGTG CAACAGGAGA TAAAGGTCCA CCTGGACCTG TGGGGCCCCC AGGCTCCAAT 
GGTCCTGTAG GGGAACCTGG ACCAGAAGGT CCAGCTGGCA ATGATGGTAC CCCAGGACGG 
GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC 
TCTCAGGGTG CCCCTGGAAC TCCTGGCCCT GTGGGTGCTC CAGGAGATGC AGGACAAAGA 
GGAGATCCGG GTTCTCGGGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGATTA 
CCTGGACCCC AAGGACCTCG TGGTGACAAA GGTGATCATG GAGACCGAGG TGACAGAGGT 
CAGAAGGGCC ACAGAGGCTT TACTGGTCTT CAGGGTCTTC CTGGCCCTCC TGGTCCAAAT 
GGTGAACAAG GAAGTGCTGG AATCCCTGGA CCATTTGGCC CAAGAGGTCC TCCAGGCCCA 
GTTGGTCCTT CAGGTAAAGA AGGAAACCCT GGGCCACTTG GGCCAATTGG ACCTCCAGGT 
GTACGAGGCA GTGTAGGAGA AGCAGGACCT GAGGGCCCTC CTGGTGAGCC TGGCCCACCT 
GGCCCTCCGG GTCCCCCTGG CCACCTTACA GCTGCTCTTG GGGATATCAT GGGGCACTAT 
GATGAAAGCA TGCCAGATCC ACTTCCTGAG TTTACTGAAG ATCAGGCGGC TCCTGATGAC 
AAAAACAAAA CGGACCCAGG GGTTCATGCT ACCCTGAAGT CACTCAGTAG TCAGATTGAA 
ACCATGCGCA GCCCCGATGG CTCGAAAAAG CACCCAGCCC GCACGTGTGA TGACCTAAAG 
CTTTGCCATT CCGCAAAGCA GAGTGGTGAA TACTGGATTG ATCCTAACCA AGGATCTGTT 
GAAGATGCAA TCAAAGTTTA CTGCAACATG GAAACAGGAG AAACATGTAT TTCAGCAAAC 
CCATCCAGTG TACCACGTAA AACCTGGTGG GCCAGTAAAT CTCCTGACAA TAAACCTGTT 
TGGTATGGTC TTGATATGAA CAGAGGGTCT CAGTTCGCTT ATGGAGACCA CCAATCACCT 
AATACAGCCA TTACTCAGAT GACTTTTTTG CGCCTTTTAT CAAAAGAAGC CTCCCAGAAC 
ATCACTTACA TCTGTAAAAA CAGTGTAGGA TACATGGACG ATCAAGCTAA GAACCTCAAA 
AAAGCTGTGG TTCTCAAAGG GGCAAATGAC TTAGATATCA AAGCAGAGGG AAATATTAGA 
TTCCGGTATA TCGTTCTTCA AGACACTTGC TCTAAGCGGA ATGGAAATGT GGGCAAGACT 
GTCTTTGAAT ATAGAACACA GAATGTGGCA CGCTTGCCCA TCATAGATCT TGCTCCTGTG 
GATGTTGGCG GCACAGACCA GGAATTCGGC GTTGAAATTG GGCCAGTTTG TTTTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A2 ( XM_011510573.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508875.1 Homo sapiens collagen type V alpha 2 (COL5A2), transcript variant X1, mRNA.

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CloneID	OHu56985
Clone ID Related Accession (Same CDS sequence)	XM_011510573.2 , XM_011510573.3
Accession Version	XM_011510573.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-2(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510573.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTAA ACAGGGACAT TTGGAAACCT GCCCCTTGTC AGATCTGTGT CTGTGACAAT 
GGAGCCATTC TCTGTGACAA GATAGAATGC CAGGATGTGC TGGACTGTGC CGACCCTGTA 
ACGCCCCCTG GGGAATGCTG TCCTGTCTGT TCACAAACAC CTGGAGGTGG CAATACCAAT 
TTTGGTAGAG GAAGAAAGGG ACAAAAGGGA GAACCAGGAT TAGTGCCTGT TGTAACAGGC 
ATACGTGGTC GTCCAGGACC GGCAGGACCT CCAGGATCAC AGGGACCAAG AGGAGAGCGA 
GGGCCAAAAG GAAGACCTGG CCCTCGTGGA CCTCAGGGAA TTGATGGAGA ACCAGGTGTT 
CCTGGTCAAC CTGGTGCTCC AGGACCTCCT GGACATCCGT CCCACCCAGG ACCCGATGGC 
TTGAGCAGGC CGTTTTCAGC TCAAATGGCT GGGTTGGATG AAAAATCTGG ACTTGGGAGT 
CAAGTAGGAC TAATGCCTGG CTCTGTGGGT CCTGTTGGCC CAAGGGGACC ACAGGGTTTA 
CAAGGACAGC AAGGTGGTGC AGGACCTACA GGACCTCCTG GTGAACCTGG TGATCCTGGA 
CCAATGGGTC CGATTGGTTC ACGTGGACCA GAGGGCCCTC CTGGTAAACC TGGGGAAGAT 
GGTGAACCTG GCAGAAATGG AAATCCTGGT GAAGTGGGAT TTGCAGGATC TCCGGGAGCT 
CGTGGATTTC CTGGGGCTCC TGGTCTTCCA GGTCTGAAGG GTCACCGAGG ACACAAAGGT 
CTTGAAGGCC CTAAAGGTGA AGTTGGAGCA CCTGGTTCCA AGGGTGAAGC TGGCCCCACT 
GGTCCAATGG GTGCCATGGG TCCTCTGGGT CCGAGGGGAA TGCCAGGAGA GAGAGGGAGA 
CTTGGGCCAC AGGGTGCTCC TGGACAACGA GGTGCACATG GTATGCCTGG AAAACCTGGA 
CCAATGGGTC CTCTTGGGAT ACCAGGCTCT TCTGGTTTTC CAGGAAATCC TGGAATGAAG 
GGAGAAGCAG GTCCTACAGG GGCGCGAGGC CCTGAAGGTC CTCAGGGGCA GAGAGGTGAA 
ACTGGGCCCC CAGGTCCAGT TGGCTCTCCA GGTCTTCCTG GTGCAATAGG AACTGATGGT 
ACTCCTGGTG CCAAAGGCCC AACGGGCTCT CCAGGTACCT CTGGTCCTCC TGGCTCAGCA 
GGGCCTCCTG GATCTCCAGG ACCTCAGGGT AGCACTGGTC CTCAGGGAAT TCGAGGCCAA 
CCGGGTGATC CAGGAGTTCC AGGTTTCAAA GGAGAAGCTG GCCCAAAAGG GGAACCAGGG 
CCACATGGTA TTCAGGGTCC GATAGGCCCA CCCGGTGAAG AAGGCAAAAG AGGTCCCAGA 
GGTGACCCAG GAACAGTTGG TCCTCCAGGG CCAGTGGGAG AAAGGGGTGC TCCTGGCAAT 
CGTGGTTTTC CAGGCTCTGA TGGTTTACCT GGGCCAAAGG GTGCTCAAGG AGAACGGGGT 
CCTGTAGGTT CTTCAGGACC CAAAGGAAGC CAGGGGGATC CAGGACGTCC AGGGGAACCT 
GGGCTTCCAG GTGCTCGGGG TTTGACAGGA AATCCTGGTG TTCAAGGTCC TGAAGGAAAA 
CTTGGACCTT TGGGTGCGCC AGGGGAAGAT GGCCGTCCAG GTCCTCCAGG CTCCATAGGA 
ATCAGAGGGC AGCCCGGGAG CATGGGCCTT CCAGGCCCCA AAGGTAGCAG TGGTGACCCT 
GGGAAACCTG GAGAAGCAGG AAATGCTGGA GTTCCTGGGC AGAGGGGAGC TCCTGGAAAA 
GATGGTGAAG TTGGTCCTTC TGGTCCTGTG GGCCCGCCGG GTCTAGCTGG TGAAAGAGGA 
GAACAAGGAC CTCCAGGCCC CACAGGTTTT CAGGGGCTTC CTGGTCCTCC AGGGCCTCCT 
GGAGAAGGTG GAAAACCAGG TGATCAAGGT GTTCCTGGAG ATCCCGGAGC AGTTGGCCCG 
TTAGGACCTA GAGGAGAACG AGGAAATCCT GGGGAAAGAG GAGAACCTGG GATAACTGGA 
CTCCCTGGTG AGAAGGGAAT GGCTGGAGGA CATGGTCCTG ATGGCCCAAA AGGCAGTCCA 
GGTCCATCTG GGACCCCTGG AGATACAGGC CCACCAGGTC TTCAAGGTAT GCCGGGAGAA 
AGAGGAATTG CAGGAACTCC TGGCCCCAAG GGTGACAGAG GTGGCATAGG AGAAAAAGGT 
GCTGAAGGCA CAGCTGGAAA TGATGGTGCA AGAGGTCTTC CAGGTCCTTT GGGCCCTCCA 
GGTCCGGCAG GTCCTACTGG AGAAAAGGGT GAACCTGGTC CTCGAGGTTT AGTTGGCCCT 
CCTGGCTCCC GGGGCAATCC TGGTTCTCGA GGTGAAAATG GGCCAACTGG AGCTGTTGGT 
TTTGCCGGAC CCCAGGGTCC TGACGGACAG CCTGGAGTAA AAGGTGAACC TGGAGAGCCA 
GGACAGAAGG GAGATGCTGG TTCTCCTGGA CCACAAGGTT TAGCAGGATC CCCTGGCCCT 
CATGGTCCTA ATGGTGTTCC TGGACTAAAA GGTGGTCGAG GAACCCAAGG TCCGCCTGGT 
GCTACAGGAT TTCCTGGTTC TGCGGGCAGA GTTGGACCTC CAGGCCCTGC TGGAGCTCCA 
GGACCTGCGG GACCCCTAGG GGAACCCGGG AAGGAGGGAC CTCCAGGTCT TCGTGGGGAC 
CCTGGCTCTC ATGGGCGTGT GGGAGATCGA GGACCAGCTG GCCCCCCTGG TGGCCCAGGA 
GACAAAGGGG ACCCAGGAGA AGATGGGCAA CCTGGTCCAG ATGGCCCCCC TGGTCCAGCT 
GGAACGACCG GGCAGAGAGG AATTGTTGGC ATGCCTGGGC AACGTGGAGA GAGAGGCATG 
CCCGGCCTAC CAGGCCCAGC GGGAACACCA GGAAAAGTAG GACCAACTGG TGCAACAGGA 
GATAAAGGTC CACCTGGACC TGTGGGGCCC CCAGGCTCCA ATGGTCCTGT AGGGGAACCT 
GGACCAGAAG GTCCAGCTGG CAATGATGGT ACCCCAGGAC GGGATGGTGC TGTTGGAGAA 
CGTGGTGATC GTGGAGACCC TGGGCCTGCA GGTCTGCCAG GCTCTCAGGG TGCCCCTGGA 
ACTCCTGGCC CTGTGGGTGC TCCAGGAGAT GCAGGACAAA GAGGAGATCC GGGTTCTCGG 
GGTCCTATAG GACCACCTGG TCGAGCTGGG AAACGTGGAT TACCTGGACC CCAAGGACCT 
CGTGGTGACA AAGGTGATCA TGGAGACCGA GGTGACAGAG GTCAGAAGGG CCACAGAGGC 
TTTACTGGTC TTCAGGGTCT TCCTGGCCCT CCTGGTCCAA ATGGTGAACA AGGAAGTGCT 
GGAATCCCTG GACCATTTGG CCCAAGAGGT CCTCCAGGCC CAGTTGGTCC TTCAGGTAAA 
GAAGGAAACC CTGGGCCACT TGGGCCAATT GGACCTCCAG GTGTACGAGG CAGTGTAGGA 
GAAGCAGGAC CTGAGGGCCC TCCTGGTGAG CCTGGCCCAC CTGGCCCTCC GGGTCCCCCT 
GGCCACCTTA CAGCTGCTCT TGGGGATATC ATGGGGCACT ATGATGAAAG CATGCCAGAT 
CCACTTCCTG AGTTTACTGA AGATCAGGCG GCTCCTGATG ACAAAAACAA AACGGACCCA 
GGGGTTCATG CTACCCTGAA GTCACTCAGT AGTCAGATTG AAACCATGCG CAGCCCCGAT 
GGCTCGAAAA AGCACCCAGC CCGCACGTGT GATGACCTAA AGCTTTGCCA TTCCGCAAAG 
CAGAGTGGTG AATACTGGAT TGATCCTAAC CAAGGATCTG TTGAAGATGC AATCAAAGTT 
TACTGCAACA TGGAAACAGG AGAAACATGT ATTTCAGCAA ACCCATCCAG TGTACCACGT 
AAAACCTGGT GGGCCAGTAA ATCTCCTGAC AATAAACCTG TTTGGTATGG TCTTGATATG 
AACAGAGGGT CTCAGTTCGC TTATGGAGAC CACCAATCAC CTAATACAGC CATTACTCAG 
ATGACTTTTT TGCGCCTTTT ATCAAAAGAA GCCTCCCAGA ACATCACTTA CATCTGTAAA 
AACAGTGTAG GATACATGGA CGATCAAGCT AAGAACCTCA AAAAAGCTGT GGTTCTCAAA 
GGGGCAAATG ACTTAGATAT CAAAGCAGAG GGAAATATTA GATTCCGGTA TATCGTTCTT 
CAAGACACTT GCTCTAAGCG GAATGGAAAT GTGGGCAAGA CTGTCTTTGA ATATAGAACA 
CAGAATGTGG CACGCTTGCC CATCATAGAT CTTGCTCCTG TGGATGTTGG CGGCACAGAC 
CAGGAATTCG GCGTTGAAAT TGGGCCAGTT TGTTTTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508875.1
CDS	196..4557
Translation	MYLNRDIWKPAPCQICVCDNGAILCDKIECQDVLDCADPVTPPGECCPVCSQTPGGGNTNFGRGRKGQKGEPGLVPVVTGIRGRPGPAGPPGSQGPRGERGPKGRPGPRGPQGIDGEPGVPGQPGAPGPPGHPSHPGPDGLSRPFSAQMAGLDEKSGLGSQVGLMPGSVGPVGPRGPQGLQGQQGGAGPTGPPGEPGDPGPMGPIGSRGPEGPPGKPGEDGEPGRNGNPGEVGFAGSPGARGFPGAPGLPGLKGHRGHKGLEGPKGEVGAPGSKGEAGPTGPMGAMGPLGPRGMPGERGRLGPQGAPGQRGAHGMPGKPGPMGPLGIPGSSGFPGNPGMKGEAGPTGARGPEGPQGQRGETGPPGPVGSPGLPGAIGTDGTPGAKGPTGSPGTSGPPGSAGPPGSPGPQGSTGPQGIRGQPGDPGVPGFKGEAGPKGEPGPHGIQGPIGPPGEEGKRGPRGDPGTVGPPGPVGERGAPGNRGFPGSDGLPGPKGAQGERGPVGSSGPKGSQGDPGRPGEPGLPGARGLTGNPGVQGPEGKLGPLGAPGEDGRPGPPGSIGIRGQPGSMGLPGPKGSSGDPGKPGEAGNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEGGKPGDQGVPGDPGAVGPLGPRGERGNPGERGEPGITGLPGEKGMAGGHGPDGPKGSPGPSGTPGDTGPPGLQGMPGERGIAGTPGPKGDRGGIGEKGAEGTAGNDGARGLPGPLGPPGPAGPTGEKGEPGPRGLVGPPGSRGNPGSRGENGPTGAVGFAGPQGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPHGPNGVPGLKGGRGTQGPPGATGFPGSAGRVGPPGPAGAPGPAGPLGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGGPGDKGDPGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKVGPTGATGDKGPPGPVGPPGSNGPVGEPGPEGPAGNDGTPGRDGAVGERGDRGDPGPAGLPGSQGAPGTPGPVGAPGDAGQRGDPGSRGPIGPPGRAGKRGLPGPQGPRGDKGDHGDRGDRGQKGHRGFTGLQGLPGPPGPNGEQGSAGIPGPFGPRGPPGPVGPSGKEGNPGPLGPIGPPGVRGSVGEAGPEGPPGEPGPPGPPGPPGHLTAALGDIMGHYDESMPDPLPEFTEDQAAPDDKNKTDPGVHATLKSLSSQIETMRSPDGSKKHPARTCDDLKLCHSAKQSGEYWIDPNQGSVEDAIKVYCNMETGETCISANPSSVPRKTWWASKSPDNKPVWYGLDMNRGSQFAYGDHQSPNTAITQMTFLRLLSKEASQNITYICKNSVGYMDDQAKNLKKAVVLKGANDLDIKAEGNIRFRYIVLQDTCSKRNGNVGKTVFEYRTQNVARLPIIDLAPVDVGGTDQEFGVEIGPVCFV

Target ORF information:

RefSeq Version	XM_011510573.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type V alpha 2 (COL5A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510573.2

ORF Insert Sequence:

ATGTACTTAA ACAGGGACAT TTGGAAACCT GCCCCTTGTC AGATCTGTGT CTGTGACAAT 
GGAGCCATTC TCTGTGACAA GATAGAATGC CAGGATGTGC TGGACTGTGC CGACCCTGTA 
ACGCCCCCTG GGGAATGCTG TCCTGTCTGT TCACAAACAC CTGGAGGTGG CAATACCAAT 
TTTGGTAGAG GAAGAAAGGG ACAAAAGGGA GAACCAGGAT TAGTGCCTGT TGTAACAGGC 
ATACGTGGTC GTCCAGGACC GGCAGGACCT CCAGGATCAC AGGGACCAAG AGGAGAGCGA 
GGGCCAAAAG GAAGACCTGG CCCTCGTGGA CCTCAGGGAA TTGATGGAGA ACCAGGTGTT 
CCTGGTCAAC CTGGTGCTCC AGGACCTCCT GGACATCCGT CCCACCCAGG ACCCGATGGC 
TTGAGCAGGC CGTTTTCAGC TCAAATGGCT GGGTTGGATG AAAAATCTGG ACTTGGGAGT 
CAAGTAGGAC TAATGCCTGG CTCTGTGGGT CCTGTTGGCC CAAGGGGACC ACAGGGTTTA 
CAAGGACAGC AAGGTGGTGC AGGACCTACA GGACCTCCTG GTGAACCTGG TGATCCTGGA 
CCAATGGGTC CGATTGGTTC ACGTGGACCA GAGGGCCCTC CTGGTAAACC TGGGGAAGAT 
GGTGAACCTG GCAGAAATGG AAATCCTGGT GAAGTGGGAT TTGCAGGATC TCCGGGAGCT 
CGTGGATTTC CTGGGGCTCC TGGTCTTCCA GGTCTGAAGG GTCACCGAGG ACACAAAGGT 
CTTGAAGGCC CTAAAGGTGA AGTTGGAGCA CCTGGTTCCA AGGGTGAAGC TGGCCCCACT 
GGTCCAATGG GTGCCATGGG TCCTCTGGGT CCGAGGGGAA TGCCAGGAGA GAGAGGGAGA 
CTTGGGCCAC AGGGTGCTCC TGGACAACGA GGTGCACATG GTATGCCTGG AAAACCTGGA 
CCAATGGGTC CTCTTGGGAT ACCAGGCTCT TCTGGTTTTC CAGGAAATCC TGGAATGAAG 
GGAGAAGCAG GTCCTACAGG GGCGCGAGGC CCTGAAGGTC CTCAGGGGCA GAGAGGTGAA 
ACTGGGCCCC CAGGTCCAGT TGGCTCTCCA GGTCTTCCTG GTGCAATAGG AACTGATGGT 
ACTCCTGGTG CCAAAGGCCC AACGGGCTCT CCAGGTACCT CTGGTCCTCC TGGCTCAGCA 
GGGCCTCCTG GATCTCCAGG ACCTCAGGGT AGCACTGGTC CTCAGGGAAT TCGAGGCCAA 
CCGGGTGATC CAGGAGTTCC AGGTTTCAAA GGAGAAGCTG GCCCAAAAGG GGAACCAGGG 
CCACATGGTA TTCAGGGTCC GATAGGCCCA CCCGGTGAAG AAGGCAAAAG AGGTCCCAGA 
GGTGACCCAG GAACAGTTGG TCCTCCAGGG CCAGTGGGAG AAAGGGGTGC TCCTGGCAAT 
CGTGGTTTTC CAGGCTCTGA TGGTTTACCT GGGCCAAAGG GTGCTCAAGG AGAACGGGGT 
CCTGTAGGTT CTTCAGGACC CAAAGGAAGC CAGGGGGATC CAGGACGTCC AGGGGAACCT 
GGGCTTCCAG GTGCTCGGGG TTTGACAGGA AATCCTGGTG TTCAAGGTCC TGAAGGAAAA 
CTTGGACCTT TGGGTGCGCC AGGGGAAGAT GGCCGTCCAG GTCCTCCAGG CTCCATAGGA 
ATCAGAGGGC AGCCCGGGAG CATGGGCCTT CCAGGCCCCA AAGGTAGCAG TGGTGACCCT 
GGGAAACCTG GAGAAGCAGG AAATGCTGGA GTTCCTGGGC AGAGGGGAGC TCCTGGAAAA 
GATGGTGAAG TTGGTCCTTC TGGTCCTGTG GGCCCGCCGG GTCTAGCTGG TGAAAGAGGA 
GAACAAGGAC CTCCAGGCCC CACAGGTTTT CAGGGGCTTC CTGGTCCTCC AGGGCCTCCT 
GGAGAAGGTG GAAAACCAGG TGATCAAGGT GTTCCTGGAG ATCCCGGAGC AGTTGGCCCG 
TTAGGACCTA GAGGAGAACG AGGAAATCCT GGGGAAAGAG GAGAACCTGG GATAACTGGA 
CTCCCTGGTG AGAAGGGAAT GGCTGGAGGA CATGGTCCTG ATGGCCCAAA AGGCAGTCCA 
GGTCCATCTG GGACCCCTGG AGATACAGGC CCACCAGGTC TTCAAGGTAT GCCGGGAGAA 
AGAGGAATTG CAGGAACTCC TGGCCCCAAG GGTGACAGAG GTGGCATAGG AGAAAAAGGT 
GCTGAAGGCA CAGCTGGAAA TGATGGTGCA AGAGGTCTTC CAGGTCCTTT GGGCCCTCCA 
GGTCCGGCAG GTCCTACTGG AGAAAAGGGT GAACCTGGTC CTCGAGGTTT AGTTGGCCCT 
CCTGGCTCCC GGGGCAATCC TGGTTCTCGA GGTGAAAATG GGCCAACTGG AGCTGTTGGT 
TTTGCCGGAC CCCAGGGTCC TGACGGACAG CCTGGAGTAA AAGGTGAACC TGGAGAGCCA 
GGACAGAAGG GAGATGCTGG TTCTCCTGGA CCACAAGGTT TAGCAGGATC CCCTGGCCCT 
CATGGTCCTA ATGGTGTTCC TGGACTAAAA GGTGGTCGAG GAACCCAAGG TCCGCCTGGT 
GCTACAGGAT TTCCTGGTTC TGCGGGCAGA GTTGGACCTC CAGGCCCTGC TGGAGCTCCA 
GGACCTGCGG GACCCCTAGG GGAACCCGGG AAGGAGGGAC CTCCAGGTCT TCGTGGGGAC 
CCTGGCTCTC ATGGGCGTGT GGGAGATCGA GGACCAGCTG GCCCCCCTGG TGGCCCAGGA 
GACAAAGGGG ACCCAGGAGA AGATGGGCAA CCTGGTCCAG ATGGCCCCCC TGGTCCAGCT 
GGAACGACCG GGCAGAGAGG AATTGTTGGC ATGCCTGGGC AACGTGGAGA GAGAGGCATG 
CCCGGCCTAC CAGGCCCAGC GGGAACACCA GGAAAAGTAG GACCAACTGG TGCAACAGGA 
GATAAAGGTC CACCTGGACC TGTGGGGCCC CCAGGCTCCA ATGGTCCTGT AGGGGAACCT 
GGACCAGAAG GTCCAGCTGG CAATGATGGT ACCCCAGGAC GGGATGGTGC TGTTGGAGAA 
CGTGGTGATC GTGGAGACCC TGGGCCTGCA GGTCTGCCAG GCTCTCAGGG TGCCCCTGGA 
ACTCCTGGCC CTGTGGGTGC TCCAGGAGAT GCAGGACAAA GAGGAGATCC GGGTTCTCGG 
GGTCCTATAG GACCACCTGG TCGAGCTGGG AAACGTGGAT TACCTGGACC CCAAGGACCT 
CGTGGTGACA AAGGTGATCA TGGAGACCGA GGTGACAGAG GTCAGAAGGG CCACAGAGGC 
TTTACTGGTC TTCAGGGTCT TCCTGGCCCT CCTGGTCCAA ATGGTGAACA AGGAAGTGCT 
GGAATCCCTG GACCATTTGG CCCAAGAGGT CCTCCAGGCC CAGTTGGTCC TTCAGGTAAA 
GAAGGAAACC CTGGGCCACT TGGGCCAATT GGACCTCCAG GTGTACGAGG CAGTGTAGGA 
GAAGCAGGAC CTGAGGGCCC TCCTGGTGAG CCTGGCCCAC CTGGCCCTCC GGGTCCCCCT 
GGCCACCTTA CAGCTGCTCT TGGGGATATC ATGGGGCACT ATGATGAAAG CATGCCAGAT 
CCACTTCCTG AGTTTACTGA AGATCAGGCG GCTCCTGATG ACAAAAACAA AACGGACCCA 
GGGGTTCATG CTACCCTGAA GTCACTCAGT AGTCAGATTG AAACCATGCG CAGCCCCGAT 
GGCTCGAAAA AGCACCCAGC CCGCACGTGT GATGACCTAA AGCTTTGCCA TTCCGCAAAG 
CAGAGTGGTG AATACTGGAT TGATCCTAAC CAAGGATCTG TTGAAGATGC AATCAAAGTT 
TACTGCAACA TGGAAACAGG AGAAACATGT ATTTCAGCAA ACCCATCCAG TGTACCACGT 
AAAACCTGGT GGGCCAGTAA ATCTCCTGAC AATAAACCTG TTTGGTATGG TCTTGATATG 
AACAGAGGGT CTCAGTTCGC TTATGGAGAC CACCAATCAC CTAATACAGC CATTACTCAG 
ATGACTTTTT TGCGCCTTTT ATCAAAAGAA GCCTCCCAGA ACATCACTTA CATCTGTAAA 
AACAGTGTAG GATACATGGA CGATCAAGCT AAGAACCTCA AAAAAGCTGT GGTTCTCAAA 
GGGGCAAATG ACTTAGATAT CAAAGCAGAG GGAAATATTA GATTCCGGTA TATCGTTCTT 
CAAGACACTT GCTCTAAGCG GAATGGAAAT GTGGGCAAGA CTGTCTTTGA ATATAGAACA 
CAGAATGTGG CACGCTTGCC CATCATAGAT CTTGCTCCTG TGGATGTTGG CGGCACAGAC 
CAGGAATTCG GCGTTGAAAT TGGGCCAGTT TGTTTTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A2 ( XM_011510573.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508875.1 Homo sapiens collagen type V alpha 2 chain (COL5A2), transcript variant X1, mRNA.

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CloneID	OHu56985
Clone ID Related Accession (Same CDS sequence)	XM_011510573.2 , XM_011510573.3
Accession Version	XM_011510573.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-2(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510573.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTAA ACAGGGACAT TTGGAAACCT GCCCCTTGTC AGATCTGTGT CTGTGACAAT 
GGAGCCATTC TCTGTGACAA GATAGAATGC CAGGATGTGC TGGACTGTGC CGACCCTGTA 
ACGCCCCCTG GGGAATGCTG TCCTGTCTGT TCACAAACAC CTGGAGGTGG CAATACCAAT 
TTTGGTAGAG GAAGAAAGGG ACAAAAGGGA GAACCAGGAT TAGTGCCTGT TGTAACAGGC 
ATACGTGGTC GTCCAGGACC GGCAGGACCT CCAGGATCAC AGGGACCAAG AGGAGAGCGA 
GGGCCAAAAG GAAGACCTGG CCCTCGTGGA CCTCAGGGAA TTGATGGAGA ACCAGGTGTT 
CCTGGTCAAC CTGGTGCTCC AGGACCTCCT GGACATCCGT CCCACCCAGG ACCCGATGGC 
TTGAGCAGGC CGTTTTCAGC TCAAATGGCT GGGTTGGATG AAAAATCTGG ACTTGGGAGT 
CAAGTAGGAC TAATGCCTGG CTCTGTGGGT CCTGTTGGCC CAAGGGGACC ACAGGGTTTA 
CAAGGACAGC AAGGTGGTGC AGGACCTACA GGACCTCCTG GTGAACCTGG TGATCCTGGA 
CCAATGGGTC CGATTGGTTC ACGTGGACCA GAGGGCCCTC CTGGTAAACC TGGGGAAGAT 
GGTGAACCTG GCAGAAATGG AAATCCTGGT GAAGTGGGAT TTGCAGGATC TCCGGGAGCT 
CGTGGATTTC CTGGGGCTCC TGGTCTTCCA GGTCTGAAGG GTCACCGAGG ACACAAAGGT 
CTTGAAGGCC CTAAAGGTGA AGTTGGAGCA CCTGGTTCCA AGGGTGAAGC TGGCCCCACT 
GGTCCAATGG GTGCCATGGG TCCTCTGGGT CCGAGGGGAA TGCCAGGAGA GAGAGGGAGA 
CTTGGGCCAC AGGGTGCTCC TGGACAACGA GGTGCACATG GTATGCCTGG AAAACCTGGA 
CCAATGGGTC CTCTTGGGAT ACCAGGCTCT TCTGGTTTTC CAGGAAATCC TGGAATGAAG 
GGAGAAGCAG GTCCTACAGG GGCGCGAGGC CCTGAAGGTC CTCAGGGGCA GAGAGGTGAA 
ACTGGGCCCC CAGGTCCAGT TGGCTCTCCA GGTCTTCCTG GTGCAATAGG AACTGATGGT 
ACTCCTGGTG CCAAAGGCCC AACGGGCTCT CCAGGTACCT CTGGTCCTCC TGGCTCAGCA 
GGGCCTCCTG GATCTCCAGG ACCTCAGGGT AGCACTGGTC CTCAGGGAAT TCGAGGCCAA 
CCGGGTGATC CAGGAGTTCC AGGTTTCAAA GGAGAAGCTG GCCCAAAAGG GGAACCAGGG 
CCACATGGTA TTCAGGGTCC GATAGGCCCA CCCGGTGAAG AAGGCAAAAG AGGTCCCAGA 
GGTGACCCAG GAACAGTTGG TCCTCCAGGG CCAGTGGGAG AAAGGGGTGC TCCTGGCAAT 
CGTGGTTTTC CAGGCTCTGA TGGTTTACCT GGGCCAAAGG GTGCTCAAGG AGAACGGGGT 
CCTGTAGGTT CTTCAGGACC CAAAGGAAGC CAGGGGGATC CAGGACGTCC AGGGGAACCT 
GGGCTTCCAG GTGCTCGGGG TTTGACAGGA AATCCTGGTG TTCAAGGTCC TGAAGGAAAA 
CTTGGACCTT TGGGTGCGCC AGGGGAAGAT GGCCGTCCAG GTCCTCCAGG CTCCATAGGA 
ATCAGAGGGC AGCCCGGGAG CATGGGCCTT CCAGGCCCCA AAGGTAGCAG TGGTGACCCT 
GGGAAACCTG GAGAAGCAGG AAATGCTGGA GTTCCTGGGC AGAGGGGAGC TCCTGGAAAA 
GATGGTGAAG TTGGTCCTTC TGGTCCTGTG GGCCCGCCGG GTCTAGCTGG TGAAAGAGGA 
GAACAAGGAC CTCCAGGCCC CACAGGTTTT CAGGGGCTTC CTGGTCCTCC AGGGCCTCCT 
GGAGAAGGTG GAAAACCAGG TGATCAAGGT GTTCCTGGAG ATCCCGGAGC AGTTGGCCCG 
TTAGGACCTA GAGGAGAACG AGGAAATCCT GGGGAAAGAG GAGAACCTGG GATAACTGGA 
CTCCCTGGTG AGAAGGGAAT GGCTGGAGGA CATGGTCCTG ATGGCCCAAA AGGCAGTCCA 
GGTCCATCTG GGACCCCTGG AGATACAGGC CCACCAGGTC TTCAAGGTAT GCCGGGAGAA 
AGAGGAATTG CAGGAACTCC TGGCCCCAAG GGTGACAGAG GTGGCATAGG AGAAAAAGGT 
GCTGAAGGCA CAGCTGGAAA TGATGGTGCA AGAGGTCTTC CAGGTCCTTT GGGCCCTCCA 
GGTCCGGCAG GTCCTACTGG AGAAAAGGGT GAACCTGGTC CTCGAGGTTT AGTTGGCCCT 
CCTGGCTCCC GGGGCAATCC TGGTTCTCGA GGTGAAAATG GGCCAACTGG AGCTGTTGGT 
TTTGCCGGAC CCCAGGGTCC TGACGGACAG CCTGGAGTAA AAGGTGAACC TGGAGAGCCA 
GGACAGAAGG GAGATGCTGG TTCTCCTGGA CCACAAGGTT TAGCAGGATC CCCTGGCCCT 
CATGGTCCTA ATGGTGTTCC TGGACTAAAA GGTGGTCGAG GAACCCAAGG TCCGCCTGGT 
GCTACAGGAT TTCCTGGTTC TGCGGGCAGA GTTGGACCTC CAGGCCCTGC TGGAGCTCCA 
GGACCTGCGG GACCCCTAGG GGAACCCGGG AAGGAGGGAC CTCCAGGTCT TCGTGGGGAC 
CCTGGCTCTC ATGGGCGTGT GGGAGATCGA GGACCAGCTG GCCCCCCTGG TGGCCCAGGA 
GACAAAGGGG ACCCAGGAGA AGATGGGCAA CCTGGTCCAG ATGGCCCCCC TGGTCCAGCT 
GGAACGACCG GGCAGAGAGG AATTGTTGGC ATGCCTGGGC AACGTGGAGA GAGAGGCATG 
CCCGGCCTAC CAGGCCCAGC GGGAACACCA GGAAAAGTAG GACCAACTGG TGCAACAGGA 
GATAAAGGTC CACCTGGACC TGTGGGGCCC CCAGGCTCCA ATGGTCCTGT AGGGGAACCT 
GGACCAGAAG GTCCAGCTGG CAATGATGGT ACCCCAGGAC GGGATGGTGC TGTTGGAGAA 
CGTGGTGATC GTGGAGACCC TGGGCCTGCA GGTCTGCCAG GCTCTCAGGG TGCCCCTGGA 
ACTCCTGGCC CTGTGGGTGC TCCAGGAGAT GCAGGACAAA GAGGAGATCC GGGTTCTCGG 
GGTCCTATAG GACCACCTGG TCGAGCTGGG AAACGTGGAT TACCTGGACC CCAAGGACCT 
CGTGGTGACA AAGGTGATCA TGGAGACCGA GGTGACAGAG GTCAGAAGGG CCACAGAGGC 
TTTACTGGTC TTCAGGGTCT TCCTGGCCCT CCTGGTCCAA ATGGTGAACA AGGAAGTGCT 
GGAATCCCTG GACCATTTGG CCCAAGAGGT CCTCCAGGCC CAGTTGGTCC TTCAGGTAAA 
GAAGGAAACC CTGGGCCACT TGGGCCAATT GGACCTCCAG GTGTACGAGG CAGTGTAGGA 
GAAGCAGGAC CTGAGGGCCC TCCTGGTGAG CCTGGCCCAC CTGGCCCTCC GGGTCCCCCT 
GGCCACCTTA CAGCTGCTCT TGGGGATATC ATGGGGCACT ATGATGAAAG CATGCCAGAT 
CCACTTCCTG AGTTTACTGA AGATCAGGCG GCTCCTGATG ACAAAAACAA AACGGACCCA 
GGGGTTCATG CTACCCTGAA GTCACTCAGT AGTCAGATTG AAACCATGCG CAGCCCCGAT 
GGCTCGAAAA AGCACCCAGC CCGCACGTGT GATGACCTAA AGCTTTGCCA TTCCGCAAAG 
CAGAGTGGTG AATACTGGAT TGATCCTAAC CAAGGATCTG TTGAAGATGC AATCAAAGTT 
TACTGCAACA TGGAAACAGG AGAAACATGT ATTTCAGCAA ACCCATCCAG TGTACCACGT 
AAAACCTGGT GGGCCAGTAA ATCTCCTGAC AATAAACCTG TTTGGTATGG TCTTGATATG 
AACAGAGGGT CTCAGTTCGC TTATGGAGAC CACCAATCAC CTAATACAGC CATTACTCAG 
ATGACTTTTT TGCGCCTTTT ATCAAAAGAA GCCTCCCAGA ACATCACTTA CATCTGTAAA 
AACAGTGTAG GATACATGGA CGATCAAGCT AAGAACCTCA AAAAAGCTGT GGTTCTCAAA 
GGGGCAAATG ACTTAGATAT CAAAGCAGAG GGAAATATTA GATTCCGGTA TATCGTTCTT 
CAAGACACTT GCTCTAAGCG GAATGGAAAT GTGGGCAAGA CTGTCTTTGA ATATAGAACA 
CAGAATGTGG CACGCTTGCC CATCATAGAT CTTGCTCCTG TGGATGTTGG CGGCACAGAC 
CAGGAATTCG GCGTTGAAAT TGGGCCAGTT TGTTTTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508875.1
CDS	196..4557
Translation	MYLNRDIWKPAPCQICVCDNGAILCDKIECQDVLDCADPVTPPGECCPVCSQTPGGGNTNFGRGRKGQKGEPGLVPVVTGIRGRPGPAGPPGSQGPRGERGPKGRPGPRGPQGIDGEPGVPGQPGAPGPPGHPSHPGPDGLSRPFSAQMAGLDEKSGLGSQVGLMPGSVGPVGPRGPQGLQGQQGGAGPTGPPGEPGDPGPMGPIGSRGPEGPPGKPGEDGEPGRNGNPGEVGFAGSPGARGFPGAPGLPGLKGHRGHKGLEGPKGEVGAPGSKGEAGPTGPMGAMGPLGPRGMPGERGRLGPQGAPGQRGAHGMPGKPGPMGPLGIPGSSGFPGNPGMKGEAGPTGARGPEGPQGQRGETGPPGPVGSPGLPGAIGTDGTPGAKGPTGSPGTSGPPGSAGPPGSPGPQGSTGPQGIRGQPGDPGVPGFKGEAGPKGEPGPHGIQGPIGPPGEEGKRGPRGDPGTVGPPGPVGERGAPGNRGFPGSDGLPGPKGAQGERGPVGSSGPKGSQGDPGRPGEPGLPGARGLTGNPGVQGPEGKLGPLGAPGEDGRPGPPGSIGIRGQPGSMGLPGPKGSSGDPGKPGEAGNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEGGKPGDQGVPGDPGAVGPLGPRGERGNPGERGEPGITGLPGEKGMAGGHGPDGPKGSPGPSGTPGDTGPPGLQGMPGERGIAGTPGPKGDRGGIGEKGAEGTAGNDGARGLPGPLGPPGPAGPTGEKGEPGPRGLVGPPGSRGNPGSRGENGPTGAVGFAGPQGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPHGPNGVPGLKGGRGTQGPPGATGFPGSAGRVGPPGPAGAPGPAGPLGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGGPGDKGDPGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKVGPTGATGDKGPPGPVGPPGSNGPVGEPGPEGPAGNDGTPGRDGAVGERGDRGDPGPAGLPGSQGAPGTPGPVGAPGDAGQRGDPGSRGPIGPPGRAGKRGLPGPQGPRGDKGDHGDRGDRGQKGHRGFTGLQGLPGPPGPNGEQGSAGIPGPFGPRGPPGPVGPSGKEGNPGPLGPIGPPGVRGSVGEAGPEGPPGEPGPPGPPGPPGHLTAALGDIMGHYDESMPDPLPEFTEDQAAPDDKNKTDPGVHATLKSLSSQIETMRSPDGSKKHPARTCDDLKLCHSAKQSGEYWIDPNQGSVEDAIKVYCNMETGETCISANPSSVPRKTWWASKSPDNKPVWYGLDMNRGSQFAYGDHQSPNTAITQMTFLRLLSKEASQNITYICKNSVGYMDDQAKNLKKAVVLKGANDLDIKAEGNIRFRYIVLQDTCSKRNGNVGKTVFEYRTQNVARLPIIDLAPVDVGGTDQEFGVEIGPVCFV

Target ORF information:

RefSeq Version	XM_011510573.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type V alpha 2 chain (COL5A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510573.3

ORF Insert Sequence:

ATGTACTTAA ACAGGGACAT TTGGAAACCT GCCCCTTGTC AGATCTGTGT CTGTGACAAT 
GGAGCCATTC TCTGTGACAA GATAGAATGC CAGGATGTGC TGGACTGTGC CGACCCTGTA 
ACGCCCCCTG GGGAATGCTG TCCTGTCTGT TCACAAACAC CTGGAGGTGG CAATACCAAT 
TTTGGTAGAG GAAGAAAGGG ACAAAAGGGA GAACCAGGAT TAGTGCCTGT TGTAACAGGC 
ATACGTGGTC GTCCAGGACC GGCAGGACCT CCAGGATCAC AGGGACCAAG AGGAGAGCGA 
GGGCCAAAAG GAAGACCTGG CCCTCGTGGA CCTCAGGGAA TTGATGGAGA ACCAGGTGTT 
CCTGGTCAAC CTGGTGCTCC AGGACCTCCT GGACATCCGT CCCACCCAGG ACCCGATGGC 
TTGAGCAGGC CGTTTTCAGC TCAAATGGCT GGGTTGGATG AAAAATCTGG ACTTGGGAGT 
CAAGTAGGAC TAATGCCTGG CTCTGTGGGT CCTGTTGGCC CAAGGGGACC ACAGGGTTTA 
CAAGGACAGC AAGGTGGTGC AGGACCTACA GGACCTCCTG GTGAACCTGG TGATCCTGGA 
CCAATGGGTC CGATTGGTTC ACGTGGACCA GAGGGCCCTC CTGGTAAACC TGGGGAAGAT 
GGTGAACCTG GCAGAAATGG AAATCCTGGT GAAGTGGGAT TTGCAGGATC TCCGGGAGCT 
CGTGGATTTC CTGGGGCTCC TGGTCTTCCA GGTCTGAAGG GTCACCGAGG ACACAAAGGT 
CTTGAAGGCC CTAAAGGTGA AGTTGGAGCA CCTGGTTCCA AGGGTGAAGC TGGCCCCACT 
GGTCCAATGG GTGCCATGGG TCCTCTGGGT CCGAGGGGAA TGCCAGGAGA GAGAGGGAGA 
CTTGGGCCAC AGGGTGCTCC TGGACAACGA GGTGCACATG GTATGCCTGG AAAACCTGGA 
CCAATGGGTC CTCTTGGGAT ACCAGGCTCT TCTGGTTTTC CAGGAAATCC TGGAATGAAG 
GGAGAAGCAG GTCCTACAGG GGCGCGAGGC CCTGAAGGTC CTCAGGGGCA GAGAGGTGAA 
ACTGGGCCCC CAGGTCCAGT TGGCTCTCCA GGTCTTCCTG GTGCAATAGG AACTGATGGT 
ACTCCTGGTG CCAAAGGCCC AACGGGCTCT CCAGGTACCT CTGGTCCTCC TGGCTCAGCA 
GGGCCTCCTG GATCTCCAGG ACCTCAGGGT AGCACTGGTC CTCAGGGAAT TCGAGGCCAA 
CCGGGTGATC CAGGAGTTCC AGGTTTCAAA GGAGAAGCTG GCCCAAAAGG GGAACCAGGG 
CCACATGGTA TTCAGGGTCC GATAGGCCCA CCCGGTGAAG AAGGCAAAAG AGGTCCCAGA 
GGTGACCCAG GAACAGTTGG TCCTCCAGGG CCAGTGGGAG AAAGGGGTGC TCCTGGCAAT 
CGTGGTTTTC CAGGCTCTGA TGGTTTACCT GGGCCAAAGG GTGCTCAAGG AGAACGGGGT 
CCTGTAGGTT CTTCAGGACC CAAAGGAAGC CAGGGGGATC CAGGACGTCC AGGGGAACCT 
GGGCTTCCAG GTGCTCGGGG TTTGACAGGA AATCCTGGTG TTCAAGGTCC TGAAGGAAAA 
CTTGGACCTT TGGGTGCGCC AGGGGAAGAT GGCCGTCCAG GTCCTCCAGG CTCCATAGGA 
ATCAGAGGGC AGCCCGGGAG CATGGGCCTT CCAGGCCCCA AAGGTAGCAG TGGTGACCCT 
GGGAAACCTG GAGAAGCAGG AAATGCTGGA GTTCCTGGGC AGAGGGGAGC TCCTGGAAAA 
GATGGTGAAG TTGGTCCTTC TGGTCCTGTG GGCCCGCCGG GTCTAGCTGG TGAAAGAGGA 
GAACAAGGAC CTCCAGGCCC CACAGGTTTT CAGGGGCTTC CTGGTCCTCC AGGGCCTCCT 
GGAGAAGGTG GAAAACCAGG TGATCAAGGT GTTCCTGGAG ATCCCGGAGC AGTTGGCCCG 
TTAGGACCTA GAGGAGAACG AGGAAATCCT GGGGAAAGAG GAGAACCTGG GATAACTGGA 
CTCCCTGGTG AGAAGGGAAT GGCTGGAGGA CATGGTCCTG ATGGCCCAAA AGGCAGTCCA 
GGTCCATCTG GGACCCCTGG AGATACAGGC CCACCAGGTC TTCAAGGTAT GCCGGGAGAA 
AGAGGAATTG CAGGAACTCC TGGCCCCAAG GGTGACAGAG GTGGCATAGG AGAAAAAGGT 
GCTGAAGGCA CAGCTGGAAA TGATGGTGCA AGAGGTCTTC CAGGTCCTTT GGGCCCTCCA 
GGTCCGGCAG GTCCTACTGG AGAAAAGGGT GAACCTGGTC CTCGAGGTTT AGTTGGCCCT 
CCTGGCTCCC GGGGCAATCC TGGTTCTCGA GGTGAAAATG GGCCAACTGG AGCTGTTGGT 
TTTGCCGGAC CCCAGGGTCC TGACGGACAG CCTGGAGTAA AAGGTGAACC TGGAGAGCCA 
GGACAGAAGG GAGATGCTGG TTCTCCTGGA CCACAAGGTT TAGCAGGATC CCCTGGCCCT 
CATGGTCCTA ATGGTGTTCC TGGACTAAAA GGTGGTCGAG GAACCCAAGG TCCGCCTGGT 
GCTACAGGAT TTCCTGGTTC TGCGGGCAGA GTTGGACCTC CAGGCCCTGC TGGAGCTCCA 
GGACCTGCGG GACCCCTAGG GGAACCCGGG AAGGAGGGAC CTCCAGGTCT TCGTGGGGAC 
CCTGGCTCTC ATGGGCGTGT GGGAGATCGA GGACCAGCTG GCCCCCCTGG TGGCCCAGGA 
GACAAAGGGG ACCCAGGAGA AGATGGGCAA CCTGGTCCAG ATGGCCCCCC TGGTCCAGCT 
GGAACGACCG GGCAGAGAGG AATTGTTGGC ATGCCTGGGC AACGTGGAGA GAGAGGCATG 
CCCGGCCTAC CAGGCCCAGC GGGAACACCA GGAAAAGTAG GACCAACTGG TGCAACAGGA 
GATAAAGGTC CACCTGGACC TGTGGGGCCC CCAGGCTCCA ATGGTCCTGT AGGGGAACCT 
GGACCAGAAG GTCCAGCTGG CAATGATGGT ACCCCAGGAC GGGATGGTGC TGTTGGAGAA 
CGTGGTGATC GTGGAGACCC TGGGCCTGCA GGTCTGCCAG GCTCTCAGGG TGCCCCTGGA 
ACTCCTGGCC CTGTGGGTGC TCCAGGAGAT GCAGGACAAA GAGGAGATCC GGGTTCTCGG 
GGTCCTATAG GACCACCTGG TCGAGCTGGG AAACGTGGAT TACCTGGACC CCAAGGACCT 
CGTGGTGACA AAGGTGATCA TGGAGACCGA GGTGACAGAG GTCAGAAGGG CCACAGAGGC 
TTTACTGGTC TTCAGGGTCT TCCTGGCCCT CCTGGTCCAA ATGGTGAACA AGGAAGTGCT 
GGAATCCCTG GACCATTTGG CCCAAGAGGT CCTCCAGGCC CAGTTGGTCC TTCAGGTAAA 
GAAGGAAACC CTGGGCCACT TGGGCCAATT GGACCTCCAG GTGTACGAGG CAGTGTAGGA 
GAAGCAGGAC CTGAGGGCCC TCCTGGTGAG CCTGGCCCAC CTGGCCCTCC GGGTCCCCCT 
GGCCACCTTA CAGCTGCTCT TGGGGATATC ATGGGGCACT ATGATGAAAG CATGCCAGAT 
CCACTTCCTG AGTTTACTGA AGATCAGGCG GCTCCTGATG ACAAAAACAA AACGGACCCA 
GGGGTTCATG CTACCCTGAA GTCACTCAGT AGTCAGATTG AAACCATGCG CAGCCCCGAT 
GGCTCGAAAA AGCACCCAGC CCGCACGTGT GATGACCTAA AGCTTTGCCA TTCCGCAAAG 
CAGAGTGGTG AATACTGGAT TGATCCTAAC CAAGGATCTG TTGAAGATGC AATCAAAGTT 
TACTGCAACA TGGAAACAGG AGAAACATGT ATTTCAGCAA ACCCATCCAG TGTACCACGT 
AAAACCTGGT GGGCCAGTAA ATCTCCTGAC AATAAACCTG TTTGGTATGG TCTTGATATG 
AACAGAGGGT CTCAGTTCGC TTATGGAGAC CACCAATCAC CTAATACAGC CATTACTCAG 
ATGACTTTTT TGCGCCTTTT ATCAAAAGAA GCCTCCCAGA ACATCACTTA CATCTGTAAA 
AACAGTGTAG GATACATGGA CGATCAAGCT AAGAACCTCA AAAAAGCTGT GGTTCTCAAA 
GGGGCAAATG ACTTAGATAT CAAAGCAGAG GGAAATATTA GATTCCGGTA TATCGTTCTT 
CAAGACACTT GCTCTAAGCG GAATGGAAAT GTGGGCAAGA CTGTCTTTGA ATATAGAACA 
CAGAATGTGG CACGCTTGCC CATCATAGAT CTTGCTCCTG TGGATGTTGG CGGCACAGAC 
CAGGAATTCG GCGTTGAAAT TGGGCCAGTT TGTTTTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spectrum of mucocutaneous, ocular and facial features and delineation of novel presentations in 62 classical Ehlers-Danlos syndrome patients.
Clinical genetics92(6)624-631(2017 Dec)
Colombi M,Dordoni C,Venturini M,Ciaccio C,Morlino S,Chiarelli N,Zanca A,Calzavara-Pinton P,Zoppi N,Castori M,Ritelli M

Proteomic characterization of human multiple myeloma bone marrow extracellular matrix.
Leukemia31(11)2426-2434(2017 Nov)
Glavey SV,Naba A,Manier S,Clauser K,Tahri S,Park J,Reagan MR,Moschetta M,Mishima Y,Gambella M,Rocci A,Sacco A,O'Dwyer ME,Asara JM,Palumbo A,Roccaro AM,Hynes RO,Ghobrial IM

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Journal of proteome research16(8)3083-3091(2017 Aug)
Naba A,Pearce OMT,Del Rosario A,Ma D,Ding H,Rajeeve V,Cutillas PR,Balkwill FR,Hynes RO

Comprehensive proteomic characterization of stem cell-derived extracellular matrices.
Biomaterials128147-159(2017 06)
Ragelle H,Naba A,Larson BL,Zhou F,Priji? M,Whittaker CA,Del Rosario A,Langer R,Hynes RO,Anderson DG

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COL5A2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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