COL5A2 cDNA ORF clone, Homo sapiens(human)

The following COL5A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL5A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu102290 NM_000393.5
Latest version!
Homo sapiens collagen type V alpha 2 chain (COL5A2), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$519.50-$727.30
$1039.00
OHu56985 XM_011510573.3
Latest version!
Homo sapiens collagen type V alpha 2 chain (COL5A2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
View Old Accession Versions >>
OHu102290 NM_000393.4 Homo sapiens collagen type V alpha 2 chain (COL5A2), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$519.50-$727.30
$1039.00
OHu56985 XM_011510573.2 Homo sapiens collagen type V alpha 2 (COL5A2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu102290
    Clone ID Related Accession (Same CDS sequence) NM_000393.4 , NM_000393.5
    Accession Version NM_000393.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-28
    Organism Homo sapiens(human)
    Product collagen alpha-2(V) chain preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL552427.3, AC133106.3, J04478.1 and AB209045.1. This sequence is a reference standard in the RefSeqGene project. On Nov 30, 2016 this sequence version replaced NM_000393.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.82255.1, AB209045.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGATGGCAA ACTGGGCGGA AGCAAGACCT CTCCTCATTC TTATTGTTTT ATTAGGGCAA 
    TTTGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC
    TGCACTCAGA ATGGCCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGTCAG
    ATCTGTGTCT GTGACAATGG AGCCATTCTC TGTGACAAGA TAGAATGCCA GGATGTGCTG
    GACTGTGCCG ACCCTGTAAC GCCCCCTGGG GAATGCTGTC CTGTCTGTTC ACAAACACCT
    GGAGGTGGCA ATACCAATTT TGGTAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA
    GTGCCTGTTG TAACAGGCAT ACGTGGTCGT CCAGGACCGG CAGGACCTCC AGGATCACAG
    GGACCAAGAG GAGAGCGAGG GCCAAAAGGA AGACCTGGCC CTCGTGGACC TCAGGGAATT
    GATGGAGAAC CAGGTGTTCC TGGTCAACCT GGTGCTCCAG GACCTCCTGG ACATCCGTCC
    CACCCAGGAC CCGATGGCTT GAGCAGGCCG TTTTCAGCTC AAATGGCTGG GTTGGATGAA
    AAATCTGGAC TTGGGAGTCA AGTAGGACTA ATGCCTGGCT CTGTGGGTCC TGTTGGCCCA
    AGGGGACCAC AGGGTTTACA AGGACAGCAA GGTGGTGCAG GACCTACAGG ACCTCCTGGT
    GAACCTGGTG ATCCTGGACC AATGGGTCCG ATTGGTTCAC GTGGACCAGA GGGCCCTCCT
    GGTAAACCTG GGGAAGATGG TGAACCTGGC AGAAATGGAA ATCCTGGTGA AGTGGGATTT
    GCAGGATCTC CGGGAGCTCG TGGATTTCCT GGGGCTCCTG GTCTTCCAGG TCTGAAGGGT
    CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCACC TGGTTCCAAG
    GGTGAAGCTG GCCCCACTGG TCCAATGGGT GCCATGGGTC CTCTGGGTCC GAGGGGAATG
    CCAGGAGAGA GAGGGAGACT TGGGCCACAG GGTGCTCCTG GACAACGAGG TGCACATGGT
    ATGCCTGGAA AACCTGGACC AATGGGTCCT CTTGGGATAC CAGGCTCTTC TGGTTTTCCA
    GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCTACAGGGG CGCGAGGCCC TGAAGGTCCT
    CAGGGGCAGA GAGGTGAAAC TGGGCCCCCA GGTCCAGTTG GCTCTCCAGG TCTTCCTGGT
    GCAATAGGAA CTGATGGTAC TCCTGGTGCC AAAGGCCCAA CGGGCTCTCC AGGTACCTCT
    GGTCCTCCTG GCTCAGCAGG GCCTCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGTCCT
    CAGGGAATTC GAGGCCAACC GGGTGATCCA GGAGTTCCAG GTTTCAAAGG AGAAGCTGGC
    CCAAAAGGGG AACCAGGGCC ACATGGTATT CAGGGTCCGA TAGGCCCACC CGGTGAAGAA
    GGCAAAAGAG GTCCCAGAGG TGACCCAGGA ACAGTTGGTC CTCCAGGGCC AGTGGGAGAA
    AGGGGTGCTC CTGGCAATCG TGGTTTTCCA GGCTCTGATG GTTTACCTGG GCCAAAGGGT
    GCTCAAGGAG AACGGGGTCC TGTAGGTTCT TCAGGACCCA AAGGAAGCCA GGGGGATCCA
    GGACGTCCAG GGGAACCTGG GCTTCCAGGT GCTCGGGGTT TGACAGGAAA TCCTGGTGTT
    CAAGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCGCCAG GGGAAGATGG CCGTCCAGGT
    CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCCGGGAGCA TGGGCCTTCC AGGCCCCAAA
    GGTAGCAGTG GTGACCCTGG GAAACCTGGA GAAGCAGGAA ATGCTGGAGT TCCTGGGCAG
    AGGGGAGCTC CTGGAAAAGA TGGTGAAGTT GGTCCTTCTG GTCCTGTGGG CCCGCCGGGT
    CTAGCTGGTG AAAGAGGAGA ACAAGGACCT CCAGGCCCCA CAGGTTTTCA GGGGCTTCCT
    GGTCCTCCAG GGCCTCCTGG AGAAGGTGGA AAACCAGGTG ATCAAGGTGT TCCTGGAGAT
    CCCGGAGCAG TTGGCCCGTT AGGACCTAGA GGAGAACGAG GAAATCCTGG GGAAAGAGGA
    GAACCTGGGA TAACTGGACT CCCTGGTGAG AAGGGAATGG CTGGAGGACA TGGTCCTGAT
    GGCCCAAAAG GCAGTCCAGG TCCATCTGGG ACCCCTGGAG ATACAGGCCC ACCAGGTCTT
    CAAGGTATGC CGGGAGAAAG AGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT
    GGCATAGGAG AAAAAGGTGC TGAAGGCACA GCTGGAAATG ATGGTGCAAG AGGTCTTCCA
    GGTCCTTTGG GCCCTCCAGG TCCGGCAGGT CCTACTGGAG AAAAGGGTGA ACCTGGTCCT
    CGAGGTTTAG TTGGCCCTCC TGGCTCCCGG GGCAATCCTG GTTCTCGAGG TGAAAATGGG
    CCAACTGGAG CTGTTGGTTT TGCCGGACCC CAGGGTCCTG ACGGACAGCC TGGAGTAAAA
    GGTGAACCTG GAGAGCCAGG ACAGAAGGGA GATGCTGGTT CTCCTGGACC ACAAGGTTTA
    GCAGGATCCC CTGGCCCTCA TGGTCCTAAT GGTGTTCCTG GACTAAAAGG TGGTCGAGGA
    ACCCAAGGTC CGCCTGGTGC TACAGGATTT CCTGGTTCTG CGGGCAGAGT TGGACCTCCA
    GGCCCTGCTG GAGCTCCAGG ACCTGCGGGA CCCCTAGGGG AACCCGGGAA GGAGGGACCT
    CCAGGTCTTC GTGGGGACCC TGGCTCTCAT GGGCGTGTGG GAGATCGAGG ACCAGCTGGC
    CCCCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGGCAACC TGGTCCAGAT
    GGCCCCCCTG GTCCAGCTGG AACGACCGGG CAGAGAGGAA TTGTTGGCAT GCCTGGGCAA
    CGTGGAGAGA GAGGCATGCC CGGCCTACCA GGCCCAGCGG GAACACCAGG AAAAGTAGGA
    CCAACTGGTG CAACAGGAGA TAAAGGTCCA CCTGGACCTG TGGGGCCCCC AGGCTCCAAT
    GGTCCTGTAG GGGAACCTGG ACCAGAAGGT CCAGCTGGCA ATGATGGTAC CCCAGGACGG
    GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC
    TCTCAGGGTG CCCCTGGAAC TCCTGGCCCT GTGGGTGCTC CAGGAGATGC AGGACAAAGA
    GGAGATCCGG GTTCTCGGGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGATTA
    CCTGGACCCC AAGGACCTCG TGGTGACAAA GGTGATCATG GAGACCGAGG TGACAGAGGT
    CAGAAGGGCC ACAGAGGCTT TACTGGTCTT CAGGGTCTTC CTGGCCCTCC TGGTCCAAAT
    GGTGAACAAG GAAGTGCTGG AATCCCTGGA CCATTTGGCC CAAGAGGTCC TCCAGGCCCA
    GTTGGTCCTT CAGGTAAAGA AGGAAACCCT GGGCCACTTG GGCCAATTGG ACCTCCAGGT
    GTACGAGGCA GTGTAGGAGA AGCAGGACCT GAGGGCCCTC CTGGTGAGCC TGGCCCACCT
    GGCCCTCCGG GTCCCCCTGG CCACCTTACA GCTGCTCTTG GGGATATCAT GGGGCACTAT
    GATGAAAGCA TGCCAGATCC ACTTCCTGAG TTTACTGAAG ATCAGGCGGC TCCTGATGAC
    AAAAACAAAA CGGACCCAGG GGTTCATGCT ACCCTGAAGT CACTCAGTAG TCAGATTGAA
    ACCATGCGCA GCCCCGATGG CTCGAAAAAG CACCCAGCCC GCACGTGTGA TGACCTAAAG
    CTTTGCCATT CCGCAAAGCA GAGTGGTGAA TACTGGATTG ATCCTAACCA AGGATCTGTT
    GAAGATGCAA TCAAAGTTTA CTGCAACATG GAAACAGGAG AAACATGTAT TTCAGCAAAC
    CCATCCAGTG TACCACGTAA AACCTGGTGG GCCAGTAAAT CTCCTGACAA TAAACCTGTT
    TGGTATGGTC TTGATATGAA CAGAGGGTCT CAGTTCGCTT ATGGAGACCA CCAATCACCT
    AATACAGCCA TTACTCAGAT GACTTTTTTG CGCCTTTTAT CAAAAGAAGC CTCCCAGAAC
    ATCACTTACA TCTGTAAAAA CAGTGTAGGA TACATGGACG ATCAAGCTAA GAACCTCAAA
    AAAGCTGTGG TTCTCAAAGG GGCAAATGAC TTAGATATCA AAGCAGAGGG AAATATTAGA
    TTCCGGTATA TCGTTCTTCA AGACACTTGC TCTAAGCGGA ATGGAAATGT GGGCAAGACT
    GTCTTTGAAT ATAGAACACA GAATGTGGCA CGCTTGCCCA TCATAGATCT TGCTCCTGTG
    GATGTTGGCG GCACAGACCA GGAATTCGGC GTTGAAATTG GGCCAGTTTG TTTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000384.2
    CDS276..4775
    Translation

    Target ORF information:

    RefSeq Version NM_000393.4
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type V alpha 2 chain (COL5A2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000393.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGATGGCAA ACTGGGCGGA AGCAAGACCT CTCCTCATTC TTATTGTTTT ATTAGGGCAA 
    TTTGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC
    TGCACTCAGA ATGGCCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGTCAG
    ATCTGTGTCT GTGACAATGG AGCCATTCTC TGTGACAAGA TAGAATGCCA GGATGTGCTG
    GACTGTGCCG ACCCTGTAAC GCCCCCTGGG GAATGCTGTC CTGTCTGTTC ACAAACACCT
    GGAGGTGGCA ATACCAATTT TGGTAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA
    GTGCCTGTTG TAACAGGCAT ACGTGGTCGT CCAGGACCGG CAGGACCTCC AGGATCACAG
    GGACCAAGAG GAGAGCGAGG GCCAAAAGGA AGACCTGGCC CTCGTGGACC TCAGGGAATT
    GATGGAGAAC CAGGTGTTCC TGGTCAACCT GGTGCTCCAG GACCTCCTGG ACATCCGTCC
    CACCCAGGAC CCGATGGCTT GAGCAGGCCG TTTTCAGCTC AAATGGCTGG GTTGGATGAA
    AAATCTGGAC TTGGGAGTCA AGTAGGACTA ATGCCTGGCT CTGTGGGTCC TGTTGGCCCA
    AGGGGACCAC AGGGTTTACA AGGACAGCAA GGTGGTGCAG GACCTACAGG ACCTCCTGGT
    GAACCTGGTG ATCCTGGACC AATGGGTCCG ATTGGTTCAC GTGGACCAGA GGGCCCTCCT
    GGTAAACCTG GGGAAGATGG TGAACCTGGC AGAAATGGAA ATCCTGGTGA AGTGGGATTT
    GCAGGATCTC CGGGAGCTCG TGGATTTCCT GGGGCTCCTG GTCTTCCAGG TCTGAAGGGT
    CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCACC TGGTTCCAAG
    GGTGAAGCTG GCCCCACTGG TCCAATGGGT GCCATGGGTC CTCTGGGTCC GAGGGGAATG
    CCAGGAGAGA GAGGGAGACT TGGGCCACAG GGTGCTCCTG GACAACGAGG TGCACATGGT
    ATGCCTGGAA AACCTGGACC AATGGGTCCT CTTGGGATAC CAGGCTCTTC TGGTTTTCCA
    GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCTACAGGGG CGCGAGGCCC TGAAGGTCCT
    CAGGGGCAGA GAGGTGAAAC TGGGCCCCCA GGTCCAGTTG GCTCTCCAGG TCTTCCTGGT
    GCAATAGGAA CTGATGGTAC TCCTGGTGCC AAAGGCCCAA CGGGCTCTCC AGGTACCTCT
    GGTCCTCCTG GCTCAGCAGG GCCTCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGTCCT
    CAGGGAATTC GAGGCCAACC GGGTGATCCA GGAGTTCCAG GTTTCAAAGG AGAAGCTGGC
    CCAAAAGGGG AACCAGGGCC ACATGGTATT CAGGGTCCGA TAGGCCCACC CGGTGAAGAA
    GGCAAAAGAG GTCCCAGAGG TGACCCAGGA ACAGTTGGTC CTCCAGGGCC AGTGGGAGAA
    AGGGGTGCTC CTGGCAATCG TGGTTTTCCA GGCTCTGATG GTTTACCTGG GCCAAAGGGT
    GCTCAAGGAG AACGGGGTCC TGTAGGTTCT TCAGGACCCA AAGGAAGCCA GGGGGATCCA
    GGACGTCCAG GGGAACCTGG GCTTCCAGGT GCTCGGGGTT TGACAGGAAA TCCTGGTGTT
    CAAGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCGCCAG GGGAAGATGG CCGTCCAGGT
    CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCCGGGAGCA TGGGCCTTCC AGGCCCCAAA
    GGTAGCAGTG GTGACCCTGG GAAACCTGGA GAAGCAGGAA ATGCTGGAGT TCCTGGGCAG
    AGGGGAGCTC CTGGAAAAGA TGGTGAAGTT GGTCCTTCTG GTCCTGTGGG CCCGCCGGGT
    CTAGCTGGTG AAAGAGGAGA ACAAGGACCT CCAGGCCCCA CAGGTTTTCA GGGGCTTCCT
    GGTCCTCCAG GGCCTCCTGG AGAAGGTGGA AAACCAGGTG ATCAAGGTGT TCCTGGAGAT
    CCCGGAGCAG TTGGCCCGTT AGGACCTAGA GGAGAACGAG GAAATCCTGG GGAAAGAGGA
    GAACCTGGGA TAACTGGACT CCCTGGTGAG AAGGGAATGG CTGGAGGACA TGGTCCTGAT
    GGCCCAAAAG GCAGTCCAGG TCCATCTGGG ACCCCTGGAG ATACAGGCCC ACCAGGTCTT
    CAAGGTATGC CGGGAGAAAG AGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT
    GGCATAGGAG AAAAAGGTGC TGAAGGCACA GCTGGAAATG ATGGTGCAAG AGGTCTTCCA
    GGTCCTTTGG GCCCTCCAGG TCCGGCAGGT CCTACTGGAG AAAAGGGTGA ACCTGGTCCT
    CGAGGTTTAG TTGGCCCTCC TGGCTCCCGG GGCAATCCTG GTTCTCGAGG TGAAAATGGG
    CCAACTGGAG CTGTTGGTTT TGCCGGACCC CAGGGTCCTG ACGGACAGCC TGGAGTAAAA
    GGTGAACCTG GAGAGCCAGG ACAGAAGGGA GATGCTGGTT CTCCTGGACC ACAAGGTTTA
    GCAGGATCCC CTGGCCCTCA TGGTCCTAAT GGTGTTCCTG GACTAAAAGG TGGTCGAGGA
    ACCCAAGGTC CGCCTGGTGC TACAGGATTT CCTGGTTCTG CGGGCAGAGT TGGACCTCCA
    GGCCCTGCTG GAGCTCCAGG ACCTGCGGGA CCCCTAGGGG AACCCGGGAA GGAGGGACCT
    CCAGGTCTTC GTGGGGACCC TGGCTCTCAT GGGCGTGTGG GAGATCGAGG ACCAGCTGGC
    CCCCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGGCAACC TGGTCCAGAT
    GGCCCCCCTG GTCCAGCTGG AACGACCGGG CAGAGAGGAA TTGTTGGCAT GCCTGGGCAA
    CGTGGAGAGA GAGGCATGCC CGGCCTACCA GGCCCAGCGG GAACACCAGG AAAAGTAGGA
    CCAACTGGTG CAACAGGAGA TAAAGGTCCA CCTGGACCTG TGGGGCCCCC AGGCTCCAAT
    GGTCCTGTAG GGGAACCTGG ACCAGAAGGT CCAGCTGGCA ATGATGGTAC CCCAGGACGG
    GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC
    TCTCAGGGTG CCCCTGGAAC TCCTGGCCCT GTGGGTGCTC CAGGAGATGC AGGACAAAGA
    GGAGATCCGG GTTCTCGGGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGATTA
    CCTGGACCCC AAGGACCTCG TGGTGACAAA GGTGATCATG GAGACCGAGG TGACAGAGGT
    CAGAAGGGCC ACAGAGGCTT TACTGGTCTT CAGGGTCTTC CTGGCCCTCC TGGTCCAAAT
    GGTGAACAAG GAAGTGCTGG AATCCCTGGA CCATTTGGCC CAAGAGGTCC TCCAGGCCCA
    GTTGGTCCTT CAGGTAAAGA AGGAAACCCT GGGCCACTTG GGCCAATTGG ACCTCCAGGT
    GTACGAGGCA GTGTAGGAGA AGCAGGACCT GAGGGCCCTC CTGGTGAGCC TGGCCCACCT
    GGCCCTCCGG GTCCCCCTGG CCACCTTACA GCTGCTCTTG GGGATATCAT GGGGCACTAT
    GATGAAAGCA TGCCAGATCC ACTTCCTGAG TTTACTGAAG ATCAGGCGGC TCCTGATGAC
    AAAAACAAAA CGGACCCAGG GGTTCATGCT ACCCTGAAGT CACTCAGTAG TCAGATTGAA
    ACCATGCGCA GCCCCGATGG CTCGAAAAAG CACCCAGCCC GCACGTGTGA TGACCTAAAG
    CTTTGCCATT CCGCAAAGCA GAGTGGTGAA TACTGGATTG ATCCTAACCA AGGATCTGTT
    GAAGATGCAA TCAAAGTTTA CTGCAACATG GAAACAGGAG AAACATGTAT TTCAGCAAAC
    CCATCCAGTG TACCACGTAA AACCTGGTGG GCCAGTAAAT CTCCTGACAA TAAACCTGTT
    TGGTATGGTC TTGATATGAA CAGAGGGTCT CAGTTCGCTT ATGGAGACCA CCAATCACCT
    AATACAGCCA TTACTCAGAT GACTTTTTTG CGCCTTTTAT CAAAAGAAGC CTCCCAGAAC
    ATCACTTACA TCTGTAAAAA CAGTGTAGGA TACATGGACG ATCAAGCTAA GAACCTCAAA
    AAAGCTGTGG TTCTCAAAGG GGCAAATGAC TTAGATATCA AAGCAGAGGG AAATATTAGA
    TTCCGGTATA TCGTTCTTCA AGACACTTGC TCTAAGCGGA ATGGAAATGT GGGCAAGACT
    GTCTTTGAAT ATAGAACACA GAATGTGGCA CGCTTGCCCA TCATAGATCT TGCTCCTGTG
    GATGTTGGCG GCACAGACCA GGAATTCGGC GTTGAAATTG GGCCAGTTTG TTTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102290
    Clone ID Related Accession (Same CDS sequence) NM_000393.4 , NM_000393.5
    Accession Version NM_000393.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product collagen alpha-2(V) chain preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL552427.3, AC133106.3, J04478.1 and AB209045.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000393.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.82255.1, AB209045.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000374866.9/ ENSP00000364000.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGATGGCAA ACTGGGCGGA AGCAAGACCT CTCCTCATTC TTATTGTTTT ATTAGGGCAA 
    TTTGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC
    TGCACTCAGA ATGGCCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGTCAG
    ATCTGTGTCT GTGACAATGG AGCCATTCTC TGTGACAAGA TAGAATGCCA GGATGTGCTG
    GACTGTGCCG ACCCTGTAAC GCCCCCTGGG GAATGCTGTC CTGTCTGTTC ACAAACACCT
    GGAGGTGGCA ATACCAATTT TGGTAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA
    GTGCCTGTTG TAACAGGCAT ACGTGGTCGT CCAGGACCGG CAGGACCTCC AGGATCACAG
    GGACCAAGAG GAGAGCGAGG GCCAAAAGGA AGACCTGGCC CTCGTGGACC TCAGGGAATT
    GATGGAGAAC CAGGTGTTCC TGGTCAACCT GGTGCTCCAG GACCTCCTGG ACATCCGTCC
    CACCCAGGAC CCGATGGCTT GAGCAGGCCG TTTTCAGCTC AAATGGCTGG GTTGGATGAA
    AAATCTGGAC TTGGGAGTCA AGTAGGACTA ATGCCTGGCT CTGTGGGTCC TGTTGGCCCA
    AGGGGACCAC AGGGTTTACA AGGACAGCAA GGTGGTGCAG GACCTACAGG ACCTCCTGGT
    GAACCTGGTG ATCCTGGACC AATGGGTCCG ATTGGTTCAC GTGGACCAGA GGGCCCTCCT
    GGTAAACCTG GGGAAGATGG TGAACCTGGC AGAAATGGAA ATCCTGGTGA AGTGGGATTT
    GCAGGATCTC CGGGAGCTCG TGGATTTCCT GGGGCTCCTG GTCTTCCAGG TCTGAAGGGT
    CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCACC TGGTTCCAAG
    GGTGAAGCTG GCCCCACTGG TCCAATGGGT GCCATGGGTC CTCTGGGTCC GAGGGGAATG
    CCAGGAGAGA GAGGGAGACT TGGGCCACAG GGTGCTCCTG GACAACGAGG TGCACATGGT
    ATGCCTGGAA AACCTGGACC AATGGGTCCT CTTGGGATAC CAGGCTCTTC TGGTTTTCCA
    GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCTACAGGGG CGCGAGGCCC TGAAGGTCCT
    CAGGGGCAGA GAGGTGAAAC TGGGCCCCCA GGTCCAGTTG GCTCTCCAGG TCTTCCTGGT
    GCAATAGGAA CTGATGGTAC TCCTGGTGCC AAAGGCCCAA CGGGCTCTCC AGGTACCTCT
    GGTCCTCCTG GCTCAGCAGG GCCTCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGTCCT
    CAGGGAATTC GAGGCCAACC GGGTGATCCA GGAGTTCCAG GTTTCAAAGG AGAAGCTGGC
    CCAAAAGGGG AACCAGGGCC ACATGGTATT CAGGGTCCGA TAGGCCCACC CGGTGAAGAA
    GGCAAAAGAG GTCCCAGAGG TGACCCAGGA ACAGTTGGTC CTCCAGGGCC AGTGGGAGAA
    AGGGGTGCTC CTGGCAATCG TGGTTTTCCA GGCTCTGATG GTTTACCTGG GCCAAAGGGT
    GCTCAAGGAG AACGGGGTCC TGTAGGTTCT TCAGGACCCA AAGGAAGCCA GGGGGATCCA
    GGACGTCCAG GGGAACCTGG GCTTCCAGGT GCTCGGGGTT TGACAGGAAA TCCTGGTGTT
    CAAGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCGCCAG GGGAAGATGG CCGTCCAGGT
    CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCCGGGAGCA TGGGCCTTCC AGGCCCCAAA
    GGTAGCAGTG GTGACCCTGG GAAACCTGGA GAAGCAGGAA ATGCTGGAGT TCCTGGGCAG
    AGGGGAGCTC CTGGAAAAGA TGGTGAAGTT GGTCCTTCTG GTCCTGTGGG CCCGCCGGGT
    CTAGCTGGTG AAAGAGGAGA ACAAGGACCT CCAGGCCCCA CAGGTTTTCA GGGGCTTCCT
    GGTCCTCCAG GGCCTCCTGG AGAAGGTGGA AAACCAGGTG ATCAAGGTGT TCCTGGAGAT
    CCCGGAGCAG TTGGCCCGTT AGGACCTAGA GGAGAACGAG GAAATCCTGG GGAAAGAGGA
    GAACCTGGGA TAACTGGACT CCCTGGTGAG AAGGGAATGG CTGGAGGACA TGGTCCTGAT
    GGCCCAAAAG GCAGTCCAGG TCCATCTGGG ACCCCTGGAG ATACAGGCCC ACCAGGTCTT
    CAAGGTATGC CGGGAGAAAG AGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT
    GGCATAGGAG AAAAAGGTGC TGAAGGCACA GCTGGAAATG ATGGTGCAAG AGGTCTTCCA
    GGTCCTTTGG GCCCTCCAGG TCCGGCAGGT CCTACTGGAG AAAAGGGTGA ACCTGGTCCT
    CGAGGTTTAG TTGGCCCTCC TGGCTCCCGG GGCAATCCTG GTTCTCGAGG TGAAAATGGG
    CCAACTGGAG CTGTTGGTTT TGCCGGACCC CAGGGTCCTG ACGGACAGCC TGGAGTAAAA
    GGTGAACCTG GAGAGCCAGG ACAGAAGGGA GATGCTGGTT CTCCTGGACC ACAAGGTTTA
    GCAGGATCCC CTGGCCCTCA TGGTCCTAAT GGTGTTCCTG GACTAAAAGG TGGTCGAGGA
    ACCCAAGGTC CGCCTGGTGC TACAGGATTT CCTGGTTCTG CGGGCAGAGT TGGACCTCCA
    GGCCCTGCTG GAGCTCCAGG ACCTGCGGGA CCCCTAGGGG AACCCGGGAA GGAGGGACCT
    CCAGGTCTTC GTGGGGACCC TGGCTCTCAT GGGCGTGTGG GAGATCGAGG ACCAGCTGGC
    CCCCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGGCAACC TGGTCCAGAT
    GGCCCCCCTG GTCCAGCTGG AACGACCGGG CAGAGAGGAA TTGTTGGCAT GCCTGGGCAA
    CGTGGAGAGA GAGGCATGCC CGGCCTACCA GGCCCAGCGG GAACACCAGG AAAAGTAGGA
    CCAACTGGTG CAACAGGAGA TAAAGGTCCA CCTGGACCTG TGGGGCCCCC AGGCTCCAAT
    GGTCCTGTAG GGGAACCTGG ACCAGAAGGT CCAGCTGGCA ATGATGGTAC CCCAGGACGG
    GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC
    TCTCAGGGTG CCCCTGGAAC TCCTGGCCCT GTGGGTGCTC CAGGAGATGC AGGACAAAGA
    GGAGATCCGG GTTCTCGGGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGATTA
    CCTGGACCCC AAGGACCTCG TGGTGACAAA GGTGATCATG GAGACCGAGG TGACAGAGGT
    CAGAAGGGCC ACAGAGGCTT TACTGGTCTT CAGGGTCTTC CTGGCCCTCC TGGTCCAAAT
    GGTGAACAAG GAAGTGCTGG AATCCCTGGA CCATTTGGCC CAAGAGGTCC TCCAGGCCCA
    GTTGGTCCTT CAGGTAAAGA AGGAAACCCT GGGCCACTTG GGCCAATTGG ACCTCCAGGT
    GTACGAGGCA GTGTAGGAGA AGCAGGACCT GAGGGCCCTC CTGGTGAGCC TGGCCCACCT
    GGCCCTCCGG GTCCCCCTGG CCACCTTACA GCTGCTCTTG GGGATATCAT GGGGCACTAT
    GATGAAAGCA TGCCAGATCC ACTTCCTGAG TTTACTGAAG ATCAGGCGGC TCCTGATGAC
    AAAAACAAAA CGGACCCAGG GGTTCATGCT ACCCTGAAGT CACTCAGTAG TCAGATTGAA
    ACCATGCGCA GCCCCGATGG CTCGAAAAAG CACCCAGCCC GCACGTGTGA TGACCTAAAG
    CTTTGCCATT CCGCAAAGCA GAGTGGTGAA TACTGGATTG ATCCTAACCA AGGATCTGTT
    GAAGATGCAA TCAAAGTTTA CTGCAACATG GAAACAGGAG AAACATGTAT TTCAGCAAAC
    CCATCCAGTG TACCACGTAA AACCTGGTGG GCCAGTAAAT CTCCTGACAA TAAACCTGTT
    TGGTATGGTC TTGATATGAA CAGAGGGTCT CAGTTCGCTT ATGGAGACCA CCAATCACCT
    AATACAGCCA TTACTCAGAT GACTTTTTTG CGCCTTTTAT CAAAAGAAGC CTCCCAGAAC
    ATCACTTACA TCTGTAAAAA CAGTGTAGGA TACATGGACG ATCAAGCTAA GAACCTCAAA
    AAAGCTGTGG TTCTCAAAGG GGCAAATGAC TTAGATATCA AAGCAGAGGG AAATATTAGA
    TTCCGGTATA TCGTTCTTCA AGACACTTGC TCTAAGCGGA ATGGAAATGT GGGCAAGACT
    GTCTTTGAAT ATAGAACACA GAATGTGGCA CGCTTGCCCA TCATAGATCT TGCTCCTGTG
    GATGTTGGCG GCACAGACCA GGAATTCGGC GTTGAAATTG GGCCAGTTTG TTTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000384.2
    CDS158..4657
    Translation

    Target ORF information:

    RefSeq Version NM_000393.5
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type V alpha 2 chain (COL5A2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000393.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGATGGCAA ACTGGGCGGA AGCAAGACCT CTCCTCATTC TTATTGTTTT ATTAGGGCAA 
    TTTGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC
    TGCACTCAGA ATGGCCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGTCAG
    ATCTGTGTCT GTGACAATGG AGCCATTCTC TGTGACAAGA TAGAATGCCA GGATGTGCTG
    GACTGTGCCG ACCCTGTAAC GCCCCCTGGG GAATGCTGTC CTGTCTGTTC ACAAACACCT
    GGAGGTGGCA ATACCAATTT TGGTAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA
    GTGCCTGTTG TAACAGGCAT ACGTGGTCGT CCAGGACCGG CAGGACCTCC AGGATCACAG
    GGACCAAGAG GAGAGCGAGG GCCAAAAGGA AGACCTGGCC CTCGTGGACC TCAGGGAATT
    GATGGAGAAC CAGGTGTTCC TGGTCAACCT GGTGCTCCAG GACCTCCTGG ACATCCGTCC
    CACCCAGGAC CCGATGGCTT GAGCAGGCCG TTTTCAGCTC AAATGGCTGG GTTGGATGAA
    AAATCTGGAC TTGGGAGTCA AGTAGGACTA ATGCCTGGCT CTGTGGGTCC TGTTGGCCCA
    AGGGGACCAC AGGGTTTACA AGGACAGCAA GGTGGTGCAG GACCTACAGG ACCTCCTGGT
    GAACCTGGTG ATCCTGGACC AATGGGTCCG ATTGGTTCAC GTGGACCAGA GGGCCCTCCT
    GGTAAACCTG GGGAAGATGG TGAACCTGGC AGAAATGGAA ATCCTGGTGA AGTGGGATTT
    GCAGGATCTC CGGGAGCTCG TGGATTTCCT GGGGCTCCTG GTCTTCCAGG TCTGAAGGGT
    CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCACC TGGTTCCAAG
    GGTGAAGCTG GCCCCACTGG TCCAATGGGT GCCATGGGTC CTCTGGGTCC GAGGGGAATG
    CCAGGAGAGA GAGGGAGACT TGGGCCACAG GGTGCTCCTG GACAACGAGG TGCACATGGT
    ATGCCTGGAA AACCTGGACC AATGGGTCCT CTTGGGATAC CAGGCTCTTC TGGTTTTCCA
    GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCTACAGGGG CGCGAGGCCC TGAAGGTCCT
    CAGGGGCAGA GAGGTGAAAC TGGGCCCCCA GGTCCAGTTG GCTCTCCAGG TCTTCCTGGT
    GCAATAGGAA CTGATGGTAC TCCTGGTGCC AAAGGCCCAA CGGGCTCTCC AGGTACCTCT
    GGTCCTCCTG GCTCAGCAGG GCCTCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGTCCT
    CAGGGAATTC GAGGCCAACC GGGTGATCCA GGAGTTCCAG GTTTCAAAGG AGAAGCTGGC
    CCAAAAGGGG AACCAGGGCC ACATGGTATT CAGGGTCCGA TAGGCCCACC CGGTGAAGAA
    GGCAAAAGAG GTCCCAGAGG TGACCCAGGA ACAGTTGGTC CTCCAGGGCC AGTGGGAGAA
    AGGGGTGCTC CTGGCAATCG TGGTTTTCCA GGCTCTGATG GTTTACCTGG GCCAAAGGGT
    GCTCAAGGAG AACGGGGTCC TGTAGGTTCT TCAGGACCCA AAGGAAGCCA GGGGGATCCA
    GGACGTCCAG GGGAACCTGG GCTTCCAGGT GCTCGGGGTT TGACAGGAAA TCCTGGTGTT
    CAAGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCGCCAG GGGAAGATGG CCGTCCAGGT
    CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCCGGGAGCA TGGGCCTTCC AGGCCCCAAA
    GGTAGCAGTG GTGACCCTGG GAAACCTGGA GAAGCAGGAA ATGCTGGAGT TCCTGGGCAG
    AGGGGAGCTC CTGGAAAAGA TGGTGAAGTT GGTCCTTCTG GTCCTGTGGG CCCGCCGGGT
    CTAGCTGGTG AAAGAGGAGA ACAAGGACCT CCAGGCCCCA CAGGTTTTCA GGGGCTTCCT
    GGTCCTCCAG GGCCTCCTGG AGAAGGTGGA AAACCAGGTG ATCAAGGTGT TCCTGGAGAT
    CCCGGAGCAG TTGGCCCGTT AGGACCTAGA GGAGAACGAG GAAATCCTGG GGAAAGAGGA
    GAACCTGGGA TAACTGGACT CCCTGGTGAG AAGGGAATGG CTGGAGGACA TGGTCCTGAT
    GGCCCAAAAG GCAGTCCAGG TCCATCTGGG ACCCCTGGAG ATACAGGCCC ACCAGGTCTT
    CAAGGTATGC CGGGAGAAAG AGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT
    GGCATAGGAG AAAAAGGTGC TGAAGGCACA GCTGGAAATG ATGGTGCAAG AGGTCTTCCA
    GGTCCTTTGG GCCCTCCAGG TCCGGCAGGT CCTACTGGAG AAAAGGGTGA ACCTGGTCCT
    CGAGGTTTAG TTGGCCCTCC TGGCTCCCGG GGCAATCCTG GTTCTCGAGG TGAAAATGGG
    CCAACTGGAG CTGTTGGTTT TGCCGGACCC CAGGGTCCTG ACGGACAGCC TGGAGTAAAA
    GGTGAACCTG GAGAGCCAGG ACAGAAGGGA GATGCTGGTT CTCCTGGACC ACAAGGTTTA
    GCAGGATCCC CTGGCCCTCA TGGTCCTAAT GGTGTTCCTG GACTAAAAGG TGGTCGAGGA
    ACCCAAGGTC CGCCTGGTGC TACAGGATTT CCTGGTTCTG CGGGCAGAGT TGGACCTCCA
    GGCCCTGCTG GAGCTCCAGG ACCTGCGGGA CCCCTAGGGG AACCCGGGAA GGAGGGACCT
    CCAGGTCTTC GTGGGGACCC TGGCTCTCAT GGGCGTGTGG GAGATCGAGG ACCAGCTGGC
    CCCCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGGCAACC TGGTCCAGAT
    GGCCCCCCTG GTCCAGCTGG AACGACCGGG CAGAGAGGAA TTGTTGGCAT GCCTGGGCAA
    CGTGGAGAGA GAGGCATGCC CGGCCTACCA GGCCCAGCGG GAACACCAGG AAAAGTAGGA
    CCAACTGGTG CAACAGGAGA TAAAGGTCCA CCTGGACCTG TGGGGCCCCC AGGCTCCAAT
    GGTCCTGTAG GGGAACCTGG ACCAGAAGGT CCAGCTGGCA ATGATGGTAC CCCAGGACGG
    GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC
    TCTCAGGGTG CCCCTGGAAC TCCTGGCCCT GTGGGTGCTC CAGGAGATGC AGGACAAAGA
    GGAGATCCGG GTTCTCGGGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGATTA
    CCTGGACCCC AAGGACCTCG TGGTGACAAA GGTGATCATG GAGACCGAGG TGACAGAGGT
    CAGAAGGGCC ACAGAGGCTT TACTGGTCTT CAGGGTCTTC CTGGCCCTCC TGGTCCAAAT
    GGTGAACAAG GAAGTGCTGG AATCCCTGGA CCATTTGGCC CAAGAGGTCC TCCAGGCCCA
    GTTGGTCCTT CAGGTAAAGA AGGAAACCCT GGGCCACTTG GGCCAATTGG ACCTCCAGGT
    GTACGAGGCA GTGTAGGAGA AGCAGGACCT GAGGGCCCTC CTGGTGAGCC TGGCCCACCT
    GGCCCTCCGG GTCCCCCTGG CCACCTTACA GCTGCTCTTG GGGATATCAT GGGGCACTAT
    GATGAAAGCA TGCCAGATCC ACTTCCTGAG TTTACTGAAG ATCAGGCGGC TCCTGATGAC
    AAAAACAAAA CGGACCCAGG GGTTCATGCT ACCCTGAAGT CACTCAGTAG TCAGATTGAA
    ACCATGCGCA GCCCCGATGG CTCGAAAAAG CACCCAGCCC GCACGTGTGA TGACCTAAAG
    CTTTGCCATT CCGCAAAGCA GAGTGGTGAA TACTGGATTG ATCCTAACCA AGGATCTGTT
    GAAGATGCAA TCAAAGTTTA CTGCAACATG GAAACAGGAG AAACATGTAT TTCAGCAAAC
    CCATCCAGTG TACCACGTAA AACCTGGTGG GCCAGTAAAT CTCCTGACAA TAAACCTGTT
    TGGTATGGTC TTGATATGAA CAGAGGGTCT CAGTTCGCTT ATGGAGACCA CCAATCACCT
    AATACAGCCA TTACTCAGAT GACTTTTTTG CGCCTTTTAT CAAAAGAAGC CTCCCAGAAC
    ATCACTTACA TCTGTAAAAA CAGTGTAGGA TACATGGACG ATCAAGCTAA GAACCTCAAA
    AAAGCTGTGG TTCTCAAAGG GGCAAATGAC TTAGATATCA AAGCAGAGGG AAATATTAGA
    TTCCGGTATA TCGTTCTTCA AGACACTTGC TCTAAGCGGA ATGGAAATGT GGGCAAGACT
    GTCTTTGAAT ATAGAACACA GAATGTGGCA CGCTTGCCCA TCATAGATCT TGCTCCTGTG
    GATGTTGGCG GCACAGACCA GGAATTCGGC GTTGAAATTG GGCCAGTTTG TTTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56985
    Clone ID Related Accession (Same CDS sequence) XM_011510573.2 , XM_011510573.3
    Accession Version XM_011510573.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product collagen alpha-2(V) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510573.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTACTTAA ACAGGGACAT TTGGAAACCT GCCCCTTGTC AGATCTGTGT CTGTGACAAT 
    GGAGCCATTC TCTGTGACAA GATAGAATGC CAGGATGTGC TGGACTGTGC CGACCCTGTA
    ACGCCCCCTG GGGAATGCTG TCCTGTCTGT TCACAAACAC CTGGAGGTGG CAATACCAAT
    TTTGGTAGAG GAAGAAAGGG ACAAAAGGGA GAACCAGGAT TAGTGCCTGT TGTAACAGGC
    ATACGTGGTC GTCCAGGACC GGCAGGACCT CCAGGATCAC AGGGACCAAG AGGAGAGCGA
    GGGCCAAAAG GAAGACCTGG CCCTCGTGGA CCTCAGGGAA TTGATGGAGA ACCAGGTGTT
    CCTGGTCAAC CTGGTGCTCC AGGACCTCCT GGACATCCGT CCCACCCAGG ACCCGATGGC
    TTGAGCAGGC CGTTTTCAGC TCAAATGGCT GGGTTGGATG AAAAATCTGG ACTTGGGAGT
    CAAGTAGGAC TAATGCCTGG CTCTGTGGGT CCTGTTGGCC CAAGGGGACC ACAGGGTTTA
    CAAGGACAGC AAGGTGGTGC AGGACCTACA GGACCTCCTG GTGAACCTGG TGATCCTGGA
    CCAATGGGTC CGATTGGTTC ACGTGGACCA GAGGGCCCTC CTGGTAAACC TGGGGAAGAT
    GGTGAACCTG GCAGAAATGG AAATCCTGGT GAAGTGGGAT TTGCAGGATC TCCGGGAGCT
    CGTGGATTTC CTGGGGCTCC TGGTCTTCCA GGTCTGAAGG GTCACCGAGG ACACAAAGGT
    CTTGAAGGCC CTAAAGGTGA AGTTGGAGCA CCTGGTTCCA AGGGTGAAGC TGGCCCCACT
    GGTCCAATGG GTGCCATGGG TCCTCTGGGT CCGAGGGGAA TGCCAGGAGA GAGAGGGAGA
    CTTGGGCCAC AGGGTGCTCC TGGACAACGA GGTGCACATG GTATGCCTGG AAAACCTGGA
    CCAATGGGTC CTCTTGGGAT ACCAGGCTCT TCTGGTTTTC CAGGAAATCC TGGAATGAAG
    GGAGAAGCAG GTCCTACAGG GGCGCGAGGC CCTGAAGGTC CTCAGGGGCA GAGAGGTGAA
    ACTGGGCCCC CAGGTCCAGT TGGCTCTCCA GGTCTTCCTG GTGCAATAGG AACTGATGGT
    ACTCCTGGTG CCAAAGGCCC AACGGGCTCT CCAGGTACCT CTGGTCCTCC TGGCTCAGCA
    GGGCCTCCTG GATCTCCAGG ACCTCAGGGT AGCACTGGTC CTCAGGGAAT TCGAGGCCAA
    CCGGGTGATC CAGGAGTTCC AGGTTTCAAA GGAGAAGCTG GCCCAAAAGG GGAACCAGGG
    CCACATGGTA TTCAGGGTCC GATAGGCCCA CCCGGTGAAG AAGGCAAAAG AGGTCCCAGA
    GGTGACCCAG GAACAGTTGG TCCTCCAGGG CCAGTGGGAG AAAGGGGTGC TCCTGGCAAT
    CGTGGTTTTC CAGGCTCTGA TGGTTTACCT GGGCCAAAGG GTGCTCAAGG AGAACGGGGT
    CCTGTAGGTT CTTCAGGACC CAAAGGAAGC CAGGGGGATC CAGGACGTCC AGGGGAACCT
    GGGCTTCCAG GTGCTCGGGG TTTGACAGGA AATCCTGGTG TTCAAGGTCC TGAAGGAAAA
    CTTGGACCTT TGGGTGCGCC AGGGGAAGAT GGCCGTCCAG GTCCTCCAGG CTCCATAGGA
    ATCAGAGGGC AGCCCGGGAG CATGGGCCTT CCAGGCCCCA AAGGTAGCAG TGGTGACCCT
    GGGAAACCTG GAGAAGCAGG AAATGCTGGA GTTCCTGGGC AGAGGGGAGC TCCTGGAAAA
    GATGGTGAAG TTGGTCCTTC TGGTCCTGTG GGCCCGCCGG GTCTAGCTGG TGAAAGAGGA
    GAACAAGGAC CTCCAGGCCC CACAGGTTTT CAGGGGCTTC CTGGTCCTCC AGGGCCTCCT
    GGAGAAGGTG GAAAACCAGG TGATCAAGGT GTTCCTGGAG ATCCCGGAGC AGTTGGCCCG
    TTAGGACCTA GAGGAGAACG AGGAAATCCT GGGGAAAGAG GAGAACCTGG GATAACTGGA
    CTCCCTGGTG AGAAGGGAAT GGCTGGAGGA CATGGTCCTG ATGGCCCAAA AGGCAGTCCA
    GGTCCATCTG GGACCCCTGG AGATACAGGC CCACCAGGTC TTCAAGGTAT GCCGGGAGAA
    AGAGGAATTG CAGGAACTCC TGGCCCCAAG GGTGACAGAG GTGGCATAGG AGAAAAAGGT
    GCTGAAGGCA CAGCTGGAAA TGATGGTGCA AGAGGTCTTC CAGGTCCTTT GGGCCCTCCA
    GGTCCGGCAG GTCCTACTGG AGAAAAGGGT GAACCTGGTC CTCGAGGTTT AGTTGGCCCT
    CCTGGCTCCC GGGGCAATCC TGGTTCTCGA GGTGAAAATG GGCCAACTGG AGCTGTTGGT
    TTTGCCGGAC CCCAGGGTCC TGACGGACAG CCTGGAGTAA AAGGTGAACC TGGAGAGCCA
    GGACAGAAGG GAGATGCTGG TTCTCCTGGA CCACAAGGTT TAGCAGGATC CCCTGGCCCT
    CATGGTCCTA ATGGTGTTCC TGGACTAAAA GGTGGTCGAG GAACCCAAGG TCCGCCTGGT
    GCTACAGGAT TTCCTGGTTC TGCGGGCAGA GTTGGACCTC CAGGCCCTGC TGGAGCTCCA
    GGACCTGCGG GACCCCTAGG GGAACCCGGG AAGGAGGGAC CTCCAGGTCT TCGTGGGGAC
    CCTGGCTCTC ATGGGCGTGT GGGAGATCGA GGACCAGCTG GCCCCCCTGG TGGCCCAGGA
    GACAAAGGGG ACCCAGGAGA AGATGGGCAA CCTGGTCCAG ATGGCCCCCC TGGTCCAGCT
    GGAACGACCG GGCAGAGAGG AATTGTTGGC ATGCCTGGGC AACGTGGAGA GAGAGGCATG
    CCCGGCCTAC CAGGCCCAGC GGGAACACCA GGAAAAGTAG GACCAACTGG TGCAACAGGA
    GATAAAGGTC CACCTGGACC TGTGGGGCCC CCAGGCTCCA ATGGTCCTGT AGGGGAACCT
    GGACCAGAAG GTCCAGCTGG CAATGATGGT ACCCCAGGAC GGGATGGTGC TGTTGGAGAA
    CGTGGTGATC GTGGAGACCC TGGGCCTGCA GGTCTGCCAG GCTCTCAGGG TGCCCCTGGA
    ACTCCTGGCC CTGTGGGTGC TCCAGGAGAT GCAGGACAAA GAGGAGATCC GGGTTCTCGG
    GGTCCTATAG GACCACCTGG TCGAGCTGGG AAACGTGGAT TACCTGGACC CCAAGGACCT
    CGTGGTGACA AAGGTGATCA TGGAGACCGA GGTGACAGAG GTCAGAAGGG CCACAGAGGC
    TTTACTGGTC TTCAGGGTCT TCCTGGCCCT CCTGGTCCAA ATGGTGAACA AGGAAGTGCT
    GGAATCCCTG GACCATTTGG CCCAAGAGGT CCTCCAGGCC CAGTTGGTCC TTCAGGTAAA
    GAAGGAAACC CTGGGCCACT TGGGCCAATT GGACCTCCAG GTGTACGAGG CAGTGTAGGA
    GAAGCAGGAC CTGAGGGCCC TCCTGGTGAG CCTGGCCCAC CTGGCCCTCC GGGTCCCCCT
    GGCCACCTTA CAGCTGCTCT TGGGGATATC ATGGGGCACT ATGATGAAAG CATGCCAGAT
    CCACTTCCTG AGTTTACTGA AGATCAGGCG GCTCCTGATG ACAAAAACAA AACGGACCCA
    GGGGTTCATG CTACCCTGAA GTCACTCAGT AGTCAGATTG AAACCATGCG CAGCCCCGAT
    GGCTCGAAAA AGCACCCAGC CCGCACGTGT GATGACCTAA AGCTTTGCCA TTCCGCAAAG
    CAGAGTGGTG AATACTGGAT TGATCCTAAC CAAGGATCTG TTGAAGATGC AATCAAAGTT
    TACTGCAACA TGGAAACAGG AGAAACATGT ATTTCAGCAA ACCCATCCAG TGTACCACGT
    AAAACCTGGT GGGCCAGTAA ATCTCCTGAC AATAAACCTG TTTGGTATGG TCTTGATATG
    AACAGAGGGT CTCAGTTCGC TTATGGAGAC CACCAATCAC CTAATACAGC CATTACTCAG
    ATGACTTTTT TGCGCCTTTT ATCAAAAGAA GCCTCCCAGA ACATCACTTA CATCTGTAAA
    AACAGTGTAG GATACATGGA CGATCAAGCT AAGAACCTCA AAAAAGCTGT GGTTCTCAAA
    GGGGCAAATG ACTTAGATAT CAAAGCAGAG GGAAATATTA GATTCCGGTA TATCGTTCTT
    CAAGACACTT GCTCTAAGCG GAATGGAAAT GTGGGCAAGA CTGTCTTTGA ATATAGAACA
    CAGAATGTGG CACGCTTGCC CATCATAGAT CTTGCTCCTG TGGATGTTGG CGGCACAGAC
    CAGGAATTCG GCGTTGAAAT TGGGCCAGTT TGTTTTGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508875.1
    CDS196..4557
    Translation

    Target ORF information:

    RefSeq Version XM_011510573.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type V alpha 2 (COL5A2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510573.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTACTTAA ACAGGGACAT TTGGAAACCT GCCCCTTGTC AGATCTGTGT CTGTGACAAT 
    GGAGCCATTC TCTGTGACAA GATAGAATGC CAGGATGTGC TGGACTGTGC CGACCCTGTA
    ACGCCCCCTG GGGAATGCTG TCCTGTCTGT TCACAAACAC CTGGAGGTGG CAATACCAAT
    TTTGGTAGAG GAAGAAAGGG ACAAAAGGGA GAACCAGGAT TAGTGCCTGT TGTAACAGGC
    ATACGTGGTC GTCCAGGACC GGCAGGACCT CCAGGATCAC AGGGACCAAG AGGAGAGCGA
    GGGCCAAAAG GAAGACCTGG CCCTCGTGGA CCTCAGGGAA TTGATGGAGA ACCAGGTGTT
    CCTGGTCAAC CTGGTGCTCC AGGACCTCCT GGACATCCGT CCCACCCAGG ACCCGATGGC
    TTGAGCAGGC CGTTTTCAGC TCAAATGGCT GGGTTGGATG AAAAATCTGG ACTTGGGAGT
    CAAGTAGGAC TAATGCCTGG CTCTGTGGGT CCTGTTGGCC CAAGGGGACC ACAGGGTTTA
    CAAGGACAGC AAGGTGGTGC AGGACCTACA GGACCTCCTG GTGAACCTGG TGATCCTGGA
    CCAATGGGTC CGATTGGTTC ACGTGGACCA GAGGGCCCTC CTGGTAAACC TGGGGAAGAT
    GGTGAACCTG GCAGAAATGG AAATCCTGGT GAAGTGGGAT TTGCAGGATC TCCGGGAGCT
    CGTGGATTTC CTGGGGCTCC TGGTCTTCCA GGTCTGAAGG GTCACCGAGG ACACAAAGGT
    CTTGAAGGCC CTAAAGGTGA AGTTGGAGCA CCTGGTTCCA AGGGTGAAGC TGGCCCCACT
    GGTCCAATGG GTGCCATGGG TCCTCTGGGT CCGAGGGGAA TGCCAGGAGA GAGAGGGAGA
    CTTGGGCCAC AGGGTGCTCC TGGACAACGA GGTGCACATG GTATGCCTGG AAAACCTGGA
    CCAATGGGTC CTCTTGGGAT ACCAGGCTCT TCTGGTTTTC CAGGAAATCC TGGAATGAAG
    GGAGAAGCAG GTCCTACAGG GGCGCGAGGC CCTGAAGGTC CTCAGGGGCA GAGAGGTGAA
    ACTGGGCCCC CAGGTCCAGT TGGCTCTCCA GGTCTTCCTG GTGCAATAGG AACTGATGGT
    ACTCCTGGTG CCAAAGGCCC AACGGGCTCT CCAGGTACCT CTGGTCCTCC TGGCTCAGCA
    GGGCCTCCTG GATCTCCAGG ACCTCAGGGT AGCACTGGTC CTCAGGGAAT TCGAGGCCAA
    CCGGGTGATC CAGGAGTTCC AGGTTTCAAA GGAGAAGCTG GCCCAAAAGG GGAACCAGGG
    CCACATGGTA TTCAGGGTCC GATAGGCCCA CCCGGTGAAG AAGGCAAAAG AGGTCCCAGA
    GGTGACCCAG GAACAGTTGG TCCTCCAGGG CCAGTGGGAG AAAGGGGTGC TCCTGGCAAT
    CGTGGTTTTC CAGGCTCTGA TGGTTTACCT GGGCCAAAGG GTGCTCAAGG AGAACGGGGT
    CCTGTAGGTT CTTCAGGACC CAAAGGAAGC CAGGGGGATC CAGGACGTCC AGGGGAACCT
    GGGCTTCCAG GTGCTCGGGG TTTGACAGGA AATCCTGGTG TTCAAGGTCC TGAAGGAAAA
    CTTGGACCTT TGGGTGCGCC AGGGGAAGAT GGCCGTCCAG GTCCTCCAGG CTCCATAGGA
    ATCAGAGGGC AGCCCGGGAG CATGGGCCTT CCAGGCCCCA AAGGTAGCAG TGGTGACCCT
    GGGAAACCTG GAGAAGCAGG AAATGCTGGA GTTCCTGGGC AGAGGGGAGC TCCTGGAAAA
    GATGGTGAAG TTGGTCCTTC TGGTCCTGTG GGCCCGCCGG GTCTAGCTGG TGAAAGAGGA
    GAACAAGGAC CTCCAGGCCC CACAGGTTTT CAGGGGCTTC CTGGTCCTCC AGGGCCTCCT
    GGAGAAGGTG GAAAACCAGG TGATCAAGGT GTTCCTGGAG ATCCCGGAGC AGTTGGCCCG
    TTAGGACCTA GAGGAGAACG AGGAAATCCT GGGGAAAGAG GAGAACCTGG GATAACTGGA
    CTCCCTGGTG AGAAGGGAAT GGCTGGAGGA CATGGTCCTG ATGGCCCAAA AGGCAGTCCA
    GGTCCATCTG GGACCCCTGG AGATACAGGC CCACCAGGTC TTCAAGGTAT GCCGGGAGAA
    AGAGGAATTG CAGGAACTCC TGGCCCCAAG GGTGACAGAG GTGGCATAGG AGAAAAAGGT
    GCTGAAGGCA CAGCTGGAAA TGATGGTGCA AGAGGTCTTC CAGGTCCTTT GGGCCCTCCA
    GGTCCGGCAG GTCCTACTGG AGAAAAGGGT GAACCTGGTC CTCGAGGTTT AGTTGGCCCT
    CCTGGCTCCC GGGGCAATCC TGGTTCTCGA GGTGAAAATG GGCCAACTGG AGCTGTTGGT
    TTTGCCGGAC CCCAGGGTCC TGACGGACAG CCTGGAGTAA AAGGTGAACC TGGAGAGCCA
    GGACAGAAGG GAGATGCTGG TTCTCCTGGA CCACAAGGTT TAGCAGGATC CCCTGGCCCT
    CATGGTCCTA ATGGTGTTCC TGGACTAAAA GGTGGTCGAG GAACCCAAGG TCCGCCTGGT
    GCTACAGGAT TTCCTGGTTC TGCGGGCAGA GTTGGACCTC CAGGCCCTGC TGGAGCTCCA
    GGACCTGCGG GACCCCTAGG GGAACCCGGG AAGGAGGGAC CTCCAGGTCT TCGTGGGGAC
    CCTGGCTCTC ATGGGCGTGT GGGAGATCGA GGACCAGCTG GCCCCCCTGG TGGCCCAGGA
    GACAAAGGGG ACCCAGGAGA AGATGGGCAA CCTGGTCCAG ATGGCCCCCC TGGTCCAGCT
    GGAACGACCG GGCAGAGAGG AATTGTTGGC ATGCCTGGGC AACGTGGAGA GAGAGGCATG
    CCCGGCCTAC CAGGCCCAGC GGGAACACCA GGAAAAGTAG GACCAACTGG TGCAACAGGA
    GATAAAGGTC CACCTGGACC TGTGGGGCCC CCAGGCTCCA ATGGTCCTGT AGGGGAACCT
    GGACCAGAAG GTCCAGCTGG CAATGATGGT ACCCCAGGAC GGGATGGTGC TGTTGGAGAA
    CGTGGTGATC GTGGAGACCC TGGGCCTGCA GGTCTGCCAG GCTCTCAGGG TGCCCCTGGA
    ACTCCTGGCC CTGTGGGTGC TCCAGGAGAT GCAGGACAAA GAGGAGATCC GGGTTCTCGG
    GGTCCTATAG GACCACCTGG TCGAGCTGGG AAACGTGGAT TACCTGGACC CCAAGGACCT
    CGTGGTGACA AAGGTGATCA TGGAGACCGA GGTGACAGAG GTCAGAAGGG CCACAGAGGC
    TTTACTGGTC TTCAGGGTCT TCCTGGCCCT CCTGGTCCAA ATGGTGAACA AGGAAGTGCT
    GGAATCCCTG GACCATTTGG CCCAAGAGGT CCTCCAGGCC CAGTTGGTCC TTCAGGTAAA
    GAAGGAAACC CTGGGCCACT TGGGCCAATT GGACCTCCAG GTGTACGAGG CAGTGTAGGA
    GAAGCAGGAC CTGAGGGCCC TCCTGGTGAG CCTGGCCCAC CTGGCCCTCC GGGTCCCCCT
    GGCCACCTTA CAGCTGCTCT TGGGGATATC ATGGGGCACT ATGATGAAAG CATGCCAGAT
    CCACTTCCTG AGTTTACTGA AGATCAGGCG GCTCCTGATG ACAAAAACAA AACGGACCCA
    GGGGTTCATG CTACCCTGAA GTCACTCAGT AGTCAGATTG AAACCATGCG CAGCCCCGAT
    GGCTCGAAAA AGCACCCAGC CCGCACGTGT GATGACCTAA AGCTTTGCCA TTCCGCAAAG
    CAGAGTGGTG AATACTGGAT TGATCCTAAC CAAGGATCTG TTGAAGATGC AATCAAAGTT
    TACTGCAACA TGGAAACAGG AGAAACATGT ATTTCAGCAA ACCCATCCAG TGTACCACGT
    AAAACCTGGT GGGCCAGTAA ATCTCCTGAC AATAAACCTG TTTGGTATGG TCTTGATATG
    AACAGAGGGT CTCAGTTCGC TTATGGAGAC CACCAATCAC CTAATACAGC CATTACTCAG
    ATGACTTTTT TGCGCCTTTT ATCAAAAGAA GCCTCCCAGA ACATCACTTA CATCTGTAAA
    AACAGTGTAG GATACATGGA CGATCAAGCT AAGAACCTCA AAAAAGCTGT GGTTCTCAAA
    GGGGCAAATG ACTTAGATAT CAAAGCAGAG GGAAATATTA GATTCCGGTA TATCGTTCTT
    CAAGACACTT GCTCTAAGCG GAATGGAAAT GTGGGCAAGA CTGTCTTTGA ATATAGAACA
    CAGAATGTGG CACGCTTGCC CATCATAGAT CTTGCTCCTG TGGATGTTGG CGGCACAGAC
    CAGGAATTCG GCGTTGAAAT TGGGCCAGTT TGTTTTGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56985
    Clone ID Related Accession (Same CDS sequence) XM_011510573.2 , XM_011510573.3
    Accession Version XM_011510573.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-2(V) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510573.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTACTTAA ACAGGGACAT TTGGAAACCT GCCCCTTGTC AGATCTGTGT CTGTGACAAT 
    GGAGCCATTC TCTGTGACAA GATAGAATGC CAGGATGTGC TGGACTGTGC CGACCCTGTA
    ACGCCCCCTG GGGAATGCTG TCCTGTCTGT TCACAAACAC CTGGAGGTGG CAATACCAAT
    TTTGGTAGAG GAAGAAAGGG ACAAAAGGGA GAACCAGGAT TAGTGCCTGT TGTAACAGGC
    ATACGTGGTC GTCCAGGACC GGCAGGACCT CCAGGATCAC AGGGACCAAG AGGAGAGCGA
    GGGCCAAAAG GAAGACCTGG CCCTCGTGGA CCTCAGGGAA TTGATGGAGA ACCAGGTGTT
    CCTGGTCAAC CTGGTGCTCC AGGACCTCCT GGACATCCGT CCCACCCAGG ACCCGATGGC
    TTGAGCAGGC CGTTTTCAGC TCAAATGGCT GGGTTGGATG AAAAATCTGG ACTTGGGAGT
    CAAGTAGGAC TAATGCCTGG CTCTGTGGGT CCTGTTGGCC CAAGGGGACC ACAGGGTTTA
    CAAGGACAGC AAGGTGGTGC AGGACCTACA GGACCTCCTG GTGAACCTGG TGATCCTGGA
    CCAATGGGTC CGATTGGTTC ACGTGGACCA GAGGGCCCTC CTGGTAAACC TGGGGAAGAT
    GGTGAACCTG GCAGAAATGG AAATCCTGGT GAAGTGGGAT TTGCAGGATC TCCGGGAGCT
    CGTGGATTTC CTGGGGCTCC TGGTCTTCCA GGTCTGAAGG GTCACCGAGG ACACAAAGGT
    CTTGAAGGCC CTAAAGGTGA AGTTGGAGCA CCTGGTTCCA AGGGTGAAGC TGGCCCCACT
    GGTCCAATGG GTGCCATGGG TCCTCTGGGT CCGAGGGGAA TGCCAGGAGA GAGAGGGAGA
    CTTGGGCCAC AGGGTGCTCC TGGACAACGA GGTGCACATG GTATGCCTGG AAAACCTGGA
    CCAATGGGTC CTCTTGGGAT ACCAGGCTCT TCTGGTTTTC CAGGAAATCC TGGAATGAAG
    GGAGAAGCAG GTCCTACAGG GGCGCGAGGC CCTGAAGGTC CTCAGGGGCA GAGAGGTGAA
    ACTGGGCCCC CAGGTCCAGT TGGCTCTCCA GGTCTTCCTG GTGCAATAGG AACTGATGGT
    ACTCCTGGTG CCAAAGGCCC AACGGGCTCT CCAGGTACCT CTGGTCCTCC TGGCTCAGCA
    GGGCCTCCTG GATCTCCAGG ACCTCAGGGT AGCACTGGTC CTCAGGGAAT TCGAGGCCAA
    CCGGGTGATC CAGGAGTTCC AGGTTTCAAA GGAGAAGCTG GCCCAAAAGG GGAACCAGGG
    CCACATGGTA TTCAGGGTCC GATAGGCCCA CCCGGTGAAG AAGGCAAAAG AGGTCCCAGA
    GGTGACCCAG GAACAGTTGG TCCTCCAGGG CCAGTGGGAG AAAGGGGTGC TCCTGGCAAT
    CGTGGTTTTC CAGGCTCTGA TGGTTTACCT GGGCCAAAGG GTGCTCAAGG AGAACGGGGT
    CCTGTAGGTT CTTCAGGACC CAAAGGAAGC CAGGGGGATC CAGGACGTCC AGGGGAACCT
    GGGCTTCCAG GTGCTCGGGG TTTGACAGGA AATCCTGGTG TTCAAGGTCC TGAAGGAAAA
    CTTGGACCTT TGGGTGCGCC AGGGGAAGAT GGCCGTCCAG GTCCTCCAGG CTCCATAGGA
    ATCAGAGGGC AGCCCGGGAG CATGGGCCTT CCAGGCCCCA AAGGTAGCAG TGGTGACCCT
    GGGAAACCTG GAGAAGCAGG AAATGCTGGA GTTCCTGGGC AGAGGGGAGC TCCTGGAAAA
    GATGGTGAAG TTGGTCCTTC TGGTCCTGTG GGCCCGCCGG GTCTAGCTGG TGAAAGAGGA
    GAACAAGGAC CTCCAGGCCC CACAGGTTTT CAGGGGCTTC CTGGTCCTCC AGGGCCTCCT
    GGAGAAGGTG GAAAACCAGG TGATCAAGGT GTTCCTGGAG ATCCCGGAGC AGTTGGCCCG
    TTAGGACCTA GAGGAGAACG AGGAAATCCT GGGGAAAGAG GAGAACCTGG GATAACTGGA
    CTCCCTGGTG AGAAGGGAAT GGCTGGAGGA CATGGTCCTG ATGGCCCAAA AGGCAGTCCA
    GGTCCATCTG GGACCCCTGG AGATACAGGC CCACCAGGTC TTCAAGGTAT GCCGGGAGAA
    AGAGGAATTG CAGGAACTCC TGGCCCCAAG GGTGACAGAG GTGGCATAGG AGAAAAAGGT
    GCTGAAGGCA CAGCTGGAAA TGATGGTGCA AGAGGTCTTC CAGGTCCTTT GGGCCCTCCA
    GGTCCGGCAG GTCCTACTGG AGAAAAGGGT GAACCTGGTC CTCGAGGTTT AGTTGGCCCT
    CCTGGCTCCC GGGGCAATCC TGGTTCTCGA GGTGAAAATG GGCCAACTGG AGCTGTTGGT
    TTTGCCGGAC CCCAGGGTCC TGACGGACAG CCTGGAGTAA AAGGTGAACC TGGAGAGCCA
    GGACAGAAGG GAGATGCTGG TTCTCCTGGA CCACAAGGTT TAGCAGGATC CCCTGGCCCT
    CATGGTCCTA ATGGTGTTCC TGGACTAAAA GGTGGTCGAG GAACCCAAGG TCCGCCTGGT
    GCTACAGGAT TTCCTGGTTC TGCGGGCAGA GTTGGACCTC CAGGCCCTGC TGGAGCTCCA
    GGACCTGCGG GACCCCTAGG GGAACCCGGG AAGGAGGGAC CTCCAGGTCT TCGTGGGGAC
    CCTGGCTCTC ATGGGCGTGT GGGAGATCGA GGACCAGCTG GCCCCCCTGG TGGCCCAGGA
    GACAAAGGGG ACCCAGGAGA AGATGGGCAA CCTGGTCCAG ATGGCCCCCC TGGTCCAGCT
    GGAACGACCG GGCAGAGAGG AATTGTTGGC ATGCCTGGGC AACGTGGAGA GAGAGGCATG
    CCCGGCCTAC CAGGCCCAGC GGGAACACCA GGAAAAGTAG GACCAACTGG TGCAACAGGA
    GATAAAGGTC CACCTGGACC TGTGGGGCCC CCAGGCTCCA ATGGTCCTGT AGGGGAACCT
    GGACCAGAAG GTCCAGCTGG CAATGATGGT ACCCCAGGAC GGGATGGTGC TGTTGGAGAA
    CGTGGTGATC GTGGAGACCC TGGGCCTGCA GGTCTGCCAG GCTCTCAGGG TGCCCCTGGA
    ACTCCTGGCC CTGTGGGTGC TCCAGGAGAT GCAGGACAAA GAGGAGATCC GGGTTCTCGG
    GGTCCTATAG GACCACCTGG TCGAGCTGGG AAACGTGGAT TACCTGGACC CCAAGGACCT
    CGTGGTGACA AAGGTGATCA TGGAGACCGA GGTGACAGAG GTCAGAAGGG CCACAGAGGC
    TTTACTGGTC TTCAGGGTCT TCCTGGCCCT CCTGGTCCAA ATGGTGAACA AGGAAGTGCT
    GGAATCCCTG GACCATTTGG CCCAAGAGGT CCTCCAGGCC CAGTTGGTCC TTCAGGTAAA
    GAAGGAAACC CTGGGCCACT TGGGCCAATT GGACCTCCAG GTGTACGAGG CAGTGTAGGA
    GAAGCAGGAC CTGAGGGCCC TCCTGGTGAG CCTGGCCCAC CTGGCCCTCC GGGTCCCCCT
    GGCCACCTTA CAGCTGCTCT TGGGGATATC ATGGGGCACT ATGATGAAAG CATGCCAGAT
    CCACTTCCTG AGTTTACTGA AGATCAGGCG GCTCCTGATG ACAAAAACAA AACGGACCCA
    GGGGTTCATG CTACCCTGAA GTCACTCAGT AGTCAGATTG AAACCATGCG CAGCCCCGAT
    GGCTCGAAAA AGCACCCAGC CCGCACGTGT GATGACCTAA AGCTTTGCCA TTCCGCAAAG
    CAGAGTGGTG AATACTGGAT TGATCCTAAC CAAGGATCTG TTGAAGATGC AATCAAAGTT
    TACTGCAACA TGGAAACAGG AGAAACATGT ATTTCAGCAA ACCCATCCAG TGTACCACGT
    AAAACCTGGT GGGCCAGTAA ATCTCCTGAC AATAAACCTG TTTGGTATGG TCTTGATATG
    AACAGAGGGT CTCAGTTCGC TTATGGAGAC CACCAATCAC CTAATACAGC CATTACTCAG
    ATGACTTTTT TGCGCCTTTT ATCAAAAGAA GCCTCCCAGA ACATCACTTA CATCTGTAAA
    AACAGTGTAG GATACATGGA CGATCAAGCT AAGAACCTCA AAAAAGCTGT GGTTCTCAAA
    GGGGCAAATG ACTTAGATAT CAAAGCAGAG GGAAATATTA GATTCCGGTA TATCGTTCTT
    CAAGACACTT GCTCTAAGCG GAATGGAAAT GTGGGCAAGA CTGTCTTTGA ATATAGAACA
    CAGAATGTGG CACGCTTGCC CATCATAGAT CTTGCTCCTG TGGATGTTGG CGGCACAGAC
    CAGGAATTCG GCGTTGAAAT TGGGCCAGTT TGTTTTGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508875.1
    CDS196..4557
    Translation

    Target ORF information:

    RefSeq Version XM_011510573.3
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type V alpha 2 chain (COL5A2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510573.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTACTTAA ACAGGGACAT TTGGAAACCT GCCCCTTGTC AGATCTGTGT CTGTGACAAT 
    GGAGCCATTC TCTGTGACAA GATAGAATGC CAGGATGTGC TGGACTGTGC CGACCCTGTA
    ACGCCCCCTG GGGAATGCTG TCCTGTCTGT TCACAAACAC CTGGAGGTGG CAATACCAAT
    TTTGGTAGAG GAAGAAAGGG ACAAAAGGGA GAACCAGGAT TAGTGCCTGT TGTAACAGGC
    ATACGTGGTC GTCCAGGACC GGCAGGACCT CCAGGATCAC AGGGACCAAG AGGAGAGCGA
    GGGCCAAAAG GAAGACCTGG CCCTCGTGGA CCTCAGGGAA TTGATGGAGA ACCAGGTGTT
    CCTGGTCAAC CTGGTGCTCC AGGACCTCCT GGACATCCGT CCCACCCAGG ACCCGATGGC
    TTGAGCAGGC CGTTTTCAGC TCAAATGGCT GGGTTGGATG AAAAATCTGG ACTTGGGAGT
    CAAGTAGGAC TAATGCCTGG CTCTGTGGGT CCTGTTGGCC CAAGGGGACC ACAGGGTTTA
    CAAGGACAGC AAGGTGGTGC AGGACCTACA GGACCTCCTG GTGAACCTGG TGATCCTGGA
    CCAATGGGTC CGATTGGTTC ACGTGGACCA GAGGGCCCTC CTGGTAAACC TGGGGAAGAT
    GGTGAACCTG GCAGAAATGG AAATCCTGGT GAAGTGGGAT TTGCAGGATC TCCGGGAGCT
    CGTGGATTTC CTGGGGCTCC TGGTCTTCCA GGTCTGAAGG GTCACCGAGG ACACAAAGGT
    CTTGAAGGCC CTAAAGGTGA AGTTGGAGCA CCTGGTTCCA AGGGTGAAGC TGGCCCCACT
    GGTCCAATGG GTGCCATGGG TCCTCTGGGT CCGAGGGGAA TGCCAGGAGA GAGAGGGAGA
    CTTGGGCCAC AGGGTGCTCC TGGACAACGA GGTGCACATG GTATGCCTGG AAAACCTGGA
    CCAATGGGTC CTCTTGGGAT ACCAGGCTCT TCTGGTTTTC CAGGAAATCC TGGAATGAAG
    GGAGAAGCAG GTCCTACAGG GGCGCGAGGC CCTGAAGGTC CTCAGGGGCA GAGAGGTGAA
    ACTGGGCCCC CAGGTCCAGT TGGCTCTCCA GGTCTTCCTG GTGCAATAGG AACTGATGGT
    ACTCCTGGTG CCAAAGGCCC AACGGGCTCT CCAGGTACCT CTGGTCCTCC TGGCTCAGCA
    GGGCCTCCTG GATCTCCAGG ACCTCAGGGT AGCACTGGTC CTCAGGGAAT TCGAGGCCAA
    CCGGGTGATC CAGGAGTTCC AGGTTTCAAA GGAGAAGCTG GCCCAAAAGG GGAACCAGGG
    CCACATGGTA TTCAGGGTCC GATAGGCCCA CCCGGTGAAG AAGGCAAAAG AGGTCCCAGA
    GGTGACCCAG GAACAGTTGG TCCTCCAGGG CCAGTGGGAG AAAGGGGTGC TCCTGGCAAT
    CGTGGTTTTC CAGGCTCTGA TGGTTTACCT GGGCCAAAGG GTGCTCAAGG AGAACGGGGT
    CCTGTAGGTT CTTCAGGACC CAAAGGAAGC CAGGGGGATC CAGGACGTCC AGGGGAACCT
    GGGCTTCCAG GTGCTCGGGG TTTGACAGGA AATCCTGGTG TTCAAGGTCC TGAAGGAAAA
    CTTGGACCTT TGGGTGCGCC AGGGGAAGAT GGCCGTCCAG GTCCTCCAGG CTCCATAGGA
    ATCAGAGGGC AGCCCGGGAG CATGGGCCTT CCAGGCCCCA AAGGTAGCAG TGGTGACCCT
    GGGAAACCTG GAGAAGCAGG AAATGCTGGA GTTCCTGGGC AGAGGGGAGC TCCTGGAAAA
    GATGGTGAAG TTGGTCCTTC TGGTCCTGTG GGCCCGCCGG GTCTAGCTGG TGAAAGAGGA
    GAACAAGGAC CTCCAGGCCC CACAGGTTTT CAGGGGCTTC CTGGTCCTCC AGGGCCTCCT
    GGAGAAGGTG GAAAACCAGG TGATCAAGGT GTTCCTGGAG ATCCCGGAGC AGTTGGCCCG
    TTAGGACCTA GAGGAGAACG AGGAAATCCT GGGGAAAGAG GAGAACCTGG GATAACTGGA
    CTCCCTGGTG AGAAGGGAAT GGCTGGAGGA CATGGTCCTG ATGGCCCAAA AGGCAGTCCA
    GGTCCATCTG GGACCCCTGG AGATACAGGC CCACCAGGTC TTCAAGGTAT GCCGGGAGAA
    AGAGGAATTG CAGGAACTCC TGGCCCCAAG GGTGACAGAG GTGGCATAGG AGAAAAAGGT
    GCTGAAGGCA CAGCTGGAAA TGATGGTGCA AGAGGTCTTC CAGGTCCTTT GGGCCCTCCA
    GGTCCGGCAG GTCCTACTGG AGAAAAGGGT GAACCTGGTC CTCGAGGTTT AGTTGGCCCT
    CCTGGCTCCC GGGGCAATCC TGGTTCTCGA GGTGAAAATG GGCCAACTGG AGCTGTTGGT
    TTTGCCGGAC CCCAGGGTCC TGACGGACAG CCTGGAGTAA AAGGTGAACC TGGAGAGCCA
    GGACAGAAGG GAGATGCTGG TTCTCCTGGA CCACAAGGTT TAGCAGGATC CCCTGGCCCT
    CATGGTCCTA ATGGTGTTCC TGGACTAAAA GGTGGTCGAG GAACCCAAGG TCCGCCTGGT
    GCTACAGGAT TTCCTGGTTC TGCGGGCAGA GTTGGACCTC CAGGCCCTGC TGGAGCTCCA
    GGACCTGCGG GACCCCTAGG GGAACCCGGG AAGGAGGGAC CTCCAGGTCT TCGTGGGGAC
    CCTGGCTCTC ATGGGCGTGT GGGAGATCGA GGACCAGCTG GCCCCCCTGG TGGCCCAGGA
    GACAAAGGGG ACCCAGGAGA AGATGGGCAA CCTGGTCCAG ATGGCCCCCC TGGTCCAGCT
    GGAACGACCG GGCAGAGAGG AATTGTTGGC ATGCCTGGGC AACGTGGAGA GAGAGGCATG
    CCCGGCCTAC CAGGCCCAGC GGGAACACCA GGAAAAGTAG GACCAACTGG TGCAACAGGA
    GATAAAGGTC CACCTGGACC TGTGGGGCCC CCAGGCTCCA ATGGTCCTGT AGGGGAACCT
    GGACCAGAAG GTCCAGCTGG CAATGATGGT ACCCCAGGAC GGGATGGTGC TGTTGGAGAA
    CGTGGTGATC GTGGAGACCC TGGGCCTGCA GGTCTGCCAG GCTCTCAGGG TGCCCCTGGA
    ACTCCTGGCC CTGTGGGTGC TCCAGGAGAT GCAGGACAAA GAGGAGATCC GGGTTCTCGG
    GGTCCTATAG GACCACCTGG TCGAGCTGGG AAACGTGGAT TACCTGGACC CCAAGGACCT
    CGTGGTGACA AAGGTGATCA TGGAGACCGA GGTGACAGAG GTCAGAAGGG CCACAGAGGC
    TTTACTGGTC TTCAGGGTCT TCCTGGCCCT CCTGGTCCAA ATGGTGAACA AGGAAGTGCT
    GGAATCCCTG GACCATTTGG CCCAAGAGGT CCTCCAGGCC CAGTTGGTCC TTCAGGTAAA
    GAAGGAAACC CTGGGCCACT TGGGCCAATT GGACCTCCAG GTGTACGAGG CAGTGTAGGA
    GAAGCAGGAC CTGAGGGCCC TCCTGGTGAG CCTGGCCCAC CTGGCCCTCC GGGTCCCCCT
    GGCCACCTTA CAGCTGCTCT TGGGGATATC ATGGGGCACT ATGATGAAAG CATGCCAGAT
    CCACTTCCTG AGTTTACTGA AGATCAGGCG GCTCCTGATG ACAAAAACAA AACGGACCCA
    GGGGTTCATG CTACCCTGAA GTCACTCAGT AGTCAGATTG AAACCATGCG CAGCCCCGAT
    GGCTCGAAAA AGCACCCAGC CCGCACGTGT GATGACCTAA AGCTTTGCCA TTCCGCAAAG
    CAGAGTGGTG AATACTGGAT TGATCCTAAC CAAGGATCTG TTGAAGATGC AATCAAAGTT
    TACTGCAACA TGGAAACAGG AGAAACATGT ATTTCAGCAA ACCCATCCAG TGTACCACGT
    AAAACCTGGT GGGCCAGTAA ATCTCCTGAC AATAAACCTG TTTGGTATGG TCTTGATATG
    AACAGAGGGT CTCAGTTCGC TTATGGAGAC CACCAATCAC CTAATACAGC CATTACTCAG
    ATGACTTTTT TGCGCCTTTT ATCAAAAGAA GCCTCCCAGA ACATCACTTA CATCTGTAAA
    AACAGTGTAG GATACATGGA CGATCAAGCT AAGAACCTCA AAAAAGCTGT GGTTCTCAAA
    GGGGCAAATG ACTTAGATAT CAAAGCAGAG GGAAATATTA GATTCCGGTA TATCGTTCTT
    CAAGACACTT GCTCTAAGCG GAATGGAAAT GTGGGCAAGA CTGTCTTTGA ATATAGAACA
    CAGAATGTGG CACGCTTGCC CATCATAGAT CTTGCTCCTG TGGATGTTGG CGGCACAGAC
    CAGGAATTCG GCGTTGAAAT TGGGCCAGTT TGTTTTGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.