Col5a2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col5a2
Entrez Gene ID	12832
Full Name	collagen, type V, alpha 2
Synonyms	1110014L14Rik
General protein information	Preferred Names collagen, type V, alpha 2 Names collagen alpha-2(V) chain procollagen, type V, alpha 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C1.1
Summary	This gene encodes the alpha-2 subunit of type V collagen, one of the low abundance fibrillar collagens that gets incorporated into growing fibrils with type I collagen. The encoded protein, in association with alpha-1 and/or alpha-3 subunits, forms homo- or heterotrimeric type V procollagen that undergoes proteolytic processing. Mice lacking the encoded protein die in utero. Transgenic mice that produce a structurally abnormal form of the encoded protein survive poorly and exhibit skin fragility, skeletal abnormalities and alterations in the collagen fiber organization. [provided by RefSeq, Dec 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007737.2	NP_031763.2	collagen alpha-2(V) chain preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	COL5A2	NP_000384.2
Pan troglodytes (chimpanzee)	COL5A2	XP_001164152.1
Canis lupus familiaris (dog)	COL5A2	XP_005640450.1
Rattus norvegicus (Norway rat)	Col5a2	NP_445940.1
Xenopus tropicalis (tropical clawed frog)	col5a2	XP_002931546.2
Danio rerio (zebrafish)	col5a2a	NP_001139254.1
Gallus gallus (chicken)	COL5A2	XP_004942453.1
	COL5A2	XP_002799008.1
Mus musculus (house mouse)	Col5a2	NP_031763.2

Gene Ontology
Bibliography

Related articles in PubMed

How aging impacts skin biomechanics: a multiscale study in mice.
Lynch B, Bonod-Bidaud C, Ducourthial G, Affagard JS, Bancelin S, Psilodimitrakopoulos S, Ruggiero F, Allain JM, Schanne-Klein MC
Scientific reports7(1)13750(2017 Oct)

Deficits in Col5a2 Expression Result in Novel Skin and Adipose Abnormalities and Predisposition to Aortic Aneurysms and Dissections.
Park AC, Phan N, Massoudi D, Liu Z, Kernien JF, Adams SM, Davidson JM, Birk DE, Liu B, Greenspan DS
The American journal of pathology187(10)2300-2311(2017 Oct)

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Col5a2 haploinsufficiency markedly increased the incidence and severity of abdominal aortic aneurysms.
Title: Deficits in Col5a2 Expression Result in Novel Skin and Adipose Abnormalities and Predisposition to Aortic Aneurysms and Dissections.

Mice with null alleles for the Col5a2. Col5a2(-/-) homozygosity is embryonic lethal at approximately 12 days post conception.
Title: Homozygosity and Heterozygosity for Null Col5a2 Alleles Produce Embryonic Lethality and a Novel Classic Ehlers-Danlos Syndrome-Related Phenotype.

analysis of processing of the Pro-alpha1(V)Pro-alpha2(V)Pro-alpha3(V) procollagen heterotrimer
Title: Biosynthetic processing of the Pro-alpha1(V)Pro-alpha2(V)Pro-alpha3(V) procollagen heterotrimer.

The following Col5a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col5a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00705	NM_007737.2 Latest version!	Mus musculus collagen, type V, alpha 2 (Col5a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col5a2 ( NM_007737.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031763.2 Mus musculus collagen, type V, alpha 2 (Col5a2), mRNA.

Price & Availability↑

CloneID	OMu00705
Clone ID Related Accession (Same CDS sequence)	NM_007737.2
Accession Version	NM_007737.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	collagen alpha-2(V) chain preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK147328.1, AK132413.1, AK139130.1 and AK003709.1. On Feb 7, 2006 this sequence version replaced NM_007737.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK139130.1, AK132413.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCAA ACTGGGTGGG AGCAAGACCT CTCCTCATTC TTTCTGTTTT ATTAGGGTAC 
TGTGTCTCAA TAAAAGCCCA GGAACAAGAG AATGATGAAT ATGATGAAGA AATAGCCTGT 
ACTCAACATG GACAGATGTA TTTAAATAGA GACATTTGGA AACCTTCTCC ATGTCAGATC 
TGTGTCTGTG ACAATGGTGC CATTCTTTGT GACAAGATAG AGTGCCCAGA GGTGCTCAAC 
TGTGCCAATC CTATAACACC CACTGGGGAG TGCTGTCCGG TGTGCCCACA AACTGGAGGT 
GGAGATACTA GTTTTGGTAG AGGAAGAAAG GGACAAAAAG GAGAACCAGG ATTAGTGCCT 
GTTGTAACAG GTATACGCGG TCGTCCAGGA CCTGCAGGCC CCCCAGGATC ACAAGGACCA 
AGAGGGGACC GAGGCCCTAA AGGAAGACCT GGTCCTCGTG GTCCTCAGGG AATTGATGGA 
GAGCCAGGTG TGCCTGGTCA GCCAGGTGCT CCAGGGCCTC CTGGGCACCC CTCTCACCCA 
GGACCTGATG GCATGAGCAG GCCTTTTTCA GCTCAGATGG CTGGGCTGGA TGAAAAATCT 
GGACTTGGAA GTCAAGTAGG ACTCATGCCT GGCTCTGTGG GTCCTGTTGG CCCAAGAGGA 
CCTCAGGGTT TACAAGGACA GCAAGGTGGT GTGGGACCCG CAGGACCTCC TGGTGAGCCT 
GGCGAGCCTG GACCAATGGG TCCAATTGGT TCTCGTGGAC CAGAGGGCCC ACCTGGGAAG 
CCCGGAGAAG ATGGTGAACC TGGAAGAAAT GGGAATACAG GTGAAGTGGG ATTCTCAGGA 
TCACCAGGTG CTCGAGGCTT CCCTGGTGCT CCTGGTCTCC CTGGCTTGAA AGGTCACAGA 
GGACACAAAG GTCTTGAAGG CCCCAAAGGT GAAATTGGAG CACCTGGTGC TAAGGGTGAA 
GCTGGCCCCA CTGGTCCAAT GGGTGCTATG GGCCCTCTGG GACCAAGAGG AATGCCAGGA 
GAGAGGGGAA GACTTGGGCC ACAGGGTGCT CCTGGAAAAC GGGGTGCCCA CGGTATGCCT 
GGAAAACCTG GACCAATGGG TCCTCTTGGG ATACCTGGCT CTTCCGGTTT TCCAGGAAAT 
CCTGGAATGA AGGGAGAAGC AGGTCCTACT GGAGCCCGAG GCCCTGAAGG TCCTCAGGGG 
CAGAGAGGTG AAACTGGGCC ACCTGGTCCA GCTGGCTCTC AGGGTCTTCC TGGTGCAGTG 
GGGACTGATG GTACACCTGG CGCCAAAGGC CCCACAGGCT CTGCAGGAAC CTCTGGCCCT 
CCTGGCTTGG CAGGGCCCCC TGGGTCTCCT GGCCCTCAAG GCAGTACTGG ACCTCAGGGA 
ATCCGAGGAC AATCGGGTGA TCCAGGAGTT CCAGGTTTCA AAGGAGAAGC TGGTCCAAAG 
GGAGAACCAG GGCCACATGG TATTCAGGGT CCCATTGGTC CACCTGGTGA AGAAGGCAAA 
AGAGGTCCTC GGGGTGATCC AGGCACAGTT GGTCCTCCAG GACCAATGGG AGAAAGGGGC 
GCTCCTGGCA ATCGTGGTTT TCCAGGGTCT GATGGTTTAC CTGGACCAAA GGGTGCTCAG 
GGAGAGCGTG GTCCTGTAGG TTCTTCGGGG CCTAAAGGAG GCCAGGGAGA TCCAGGACGT 
CCAGGAGAAC CTGGGCTTCC AGGTGCTCGG GGTCTGACAG GAAATCCTGG TGTTCAAGGT 
CCTGAAGGAA AACTTGGGCC TTTGGGTGCT CCTGGGGAAG ATGGTCGGCC AGGTCCTCCA 
GGCTCCATTG GGATCAGAGG ACAGCCTGGA AGCATGGGCC TTCCAGGCCC CAAAGGTAGC 
AGTGGTGACC TTGGAAAACC AGGAGAAGCA GGAAATGCTG GTGTGCCTGG GCAAAGGGGG 
GCTCCTGGCA AAGATGGAGA AGTTGGTCCT TCAGGCCCTG TGGGACCTCC GGGCCTAGCT 
GGGGAAAGAG GAGAGCAGGG ACCTCCAGGC CCCACTGGCT TTCAGGGACT TCCTGGACCT 
CCAGGGCCAC CTGGAGAAGG TGGAAAGGCT GGTGATCAAG GTGTTCCTGG AGAGCCTGGA 
GCAGTTGGCC CATTAGGACC TCGGGGAGAA AGAGGGAATC CTGGAGAAAG AGGAGAGCCA 
GGGATAACTG GACTGCCTGG GGAGAAAGGC ATGGCTGGAG GACATGGCCC TGATGGTCCA 
AAGGGCAACC CAGGTCCAAC TGGGACCATT GGAGACACAG GGCCACCAGG TCTTCAGGGC 
ATGCCAGGAG AAAGAGGAAT TGCAGGAACA CCTGGCCCTA AGGGTGACCG AGGTGGCATA 
GGAGAGAAAG GTGCTGAAGG AACAGCAGGA AATGACGGGG CAAGGGGTCT TCCAGGTCCC 
TTGGGACCTC CAGGTCCTGC AGGCCCTACT GGAGAAAAGG GTGAACCTGG TCCTAGAGGC 
TTAGTTGGCC CTCCTGGCTC CCGTGGCAAT CCTGGTTCTC GTGGTGAAAA TGGTCCAACT 
GGAGCTGTTG GTTTTGCTGG ACCCCAGGGT CCCGATGGAC AGCCTGGTGT CAAGGGTGAG 
CCTGGAGAGC CAGGACAGAA GGGAGATGCT GGGTCTCCTG GACCCCAAGG GCTAGCAGGA 
TCACCTGGCC CCCATGGTCC ACATGGTGTT CCTGGACTGA AAGGCGGTAG AGGAACCCAG 
GGTCCACCTG GTGCTACAGG GTTTCCTGGT TCTGCTGGCA GAGTTGGGCC TCCAGGCCCT 
GCTGGAGCTC CAGGACCTGC AGGGCCCGCA GGAGAACCTG GGAAGGAAGG ACCTCCTGGT 
CTTCGTGGTG ACCCTGGCTC TCATGGGCGA GTGGGAGATA GAGGACCAGC TGGCCCACCT 
GGTAGCCCAG GAGATAAAGG GGACCCAGGA GAAGATGGAC AACCTGGTCC AGATGGTCCT 
CCTGGTCCCG CTGGTACCAC TGGGCAAAGA GGAATTGTTG GAATGCCCGG ACAACGTGGG 
GAACGAGGAA TGCCTGGCCT TCCTGGCCCA GCGGGTACAC CAGGAAAAGT AGGACCAACT 
GGTGCGACAG GAGATAAAGG TCCCCCTGGA CCTGTAGGAC CTCCAGGTTC CAATGGTCCT 
GTAGGAGAAC CTGGGCCAGA AGGTCCGGCT GGTAATGATG GTACCCCAGG ACGGGATGGT 
GCTGTTGGAG AACGGGGTGA CCGTGGAGAC CCCGGGCCTG CTGGTCTACC AGGCTCTCAG 
GGTGCCCCTG GAACTCCTGG CCCTGTTGGT GCCCCTGGAG ATGCAGGACA AAGAGGAGAG 
CCAGGTTCTC GAGGCCCTGT AGGACCACCT GGTCGAGCTG GAAAGCGTGG TTTACCTGGA 
CCACAAGGAC CTCGAGGTGA CAAGGGTGAC AATGGAGACA GAGGTGACCG AGGTCAGAAA 
GGCCACAGAG GGTTCACTGG CCTGCAGGGT CTTCCTGGAC CTCCTGGTCC AAATGGTGAA 
CAAGGAAGTG CTGGCATTCC TGGTCCTTTC GGTCCAAGAG GTCCTCCAGG ACCAGTAGGT 
CCTTCAGGAA AGGAAGGAAA CCCTGGGCCA CTTGGACCAA TTGGACCTCC TGGTGTGCGG 
GGCAGTGTAG GAGAAGCAGG ACCAGAGGGT CCTCCTGGTG AACCTGGGCC TCCTGGCCCT 
CCAGGTCCCC CTGGCCATCT TACTGCTGCT CTTGGTGATA TCATGGGACA CTATGATGAG 
AACATGCCAG ATCCACTTCC AGAGTTTACG GAAGACCAGG CAGCTCCAGA TGACACAAAC 
AAAACAGACC CTGGGATCCA TGTTACCCTG AAGTCTCTCA GTAGTCAGAT TGAAACCATG 
CGTAGCCCTG ATGGTTCCAA GAAACACCCA GCCCGCACTT GTGATGATTT AAAGCTTTGC 
CATCCCACAA AGCAGAGCGG TGAATATTGG ATTGATCCTA ACCAGGGATC AGCTGAAGAT 
GCAATCAAAG TTTACTGCAA TATGGAAACA GGAGAGACAT GTATTTCAGC AAACCCAGCC 
AGTGTTCCAC GTAAAACCTG GTGGGCCAGC AAATCCCCTG ACAATAAGCC TGTGTGGTAC 
GGTCTTGACA TGAATAGAGG ATCTCAGTTC ACATATGGAG ATTACCAGTC TCCTAACACA 
GCTATCACTC AGATGACCTT TTTGCGTCTT TTATCAAAAG AAGCCTCGCA GAACCTTACT 
TACATCTGTA GGAACACTGT TGGATATATG GATGATCAAG CTAAAAACCT CAAGAAAGCT 
GTGGTCCTTA AAGGGTCAAA CGACTTAGAA ATAAAAGGAG AAGGAAACAT CAGATTCAGA 
TACACAGTTC TTCAAGACAC CTGCTCTAAG CGAAATGGAA ATGTAGGCAA AACTATCTTT 
GAATACAGAA CACAGAATGT GGCCCGCTTG CCCATCATAG ATGTTGGTCC TGTGGATATT 
GGCAATGCAG ACCAGGAATT TGGCCTTGAT ATTGGGCCAG TTTGTTTCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031763.2
CDS	369..4862
Translation	MMANWVGARPLLILSVLLGYCVSIKAQEQENDEYDEEIACTQHGQMYLNRDIWKPSPCQICVCDNGAILCDKIECPEVLNCANPITPTGECCPVCPQTGGGDTSFGRGRKGQKGEPGLVPVVTGIRGRPGPAGPPGSQGPRGDRGPKGRPGPRGPQGIDGEPGVPGQPGAPGPPGHPSHPGPDGMSRPFSAQMAGLDEKSGLGSQVGLMPGSVGPVGPRGPQGLQGQQGGVGPAGPPGEPGEPGPMGPIGSRGPEGPPGKPGEDGEPGRNGNTGEVGFSGSPGARGFPGAPGLPGLKGHRGHKGLEGPKGEIGAPGAKGEAGPTGPMGAMGPLGPRGMPGERGRLGPQGAPGKRGAHGMPGKPGPMGPLGIPGSSGFPGNPGMKGEAGPTGARGPEGPQGQRGETGPPGPAGSQGLPGAVGTDGTPGAKGPTGSAGTSGPPGLAGPPGSPGPQGSTGPQGIRGQSGDPGVPGFKGEAGPKGEPGPHGIQGPIGPPGEEGKRGPRGDPGTVGPPGPMGERGAPGNRGFPGSDGLPGPKGAQGERGPVGSSGPKGGQGDPGRPGEPGLPGARGLTGNPGVQGPEGKLGPLGAPGEDGRPGPPGSIGIRGQPGSMGLPGPKGSSGDLGKPGEAGNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEGGKAGDQGVPGEPGAVGPLGPRGERGNPGERGEPGITGLPGEKGMAGGHGPDGPKGNPGPTGTIGDTGPPGLQGMPGERGIAGTPGPKGDRGGIGEKGAEGTAGNDGARGLPGPLGPPGPAGPTGEKGEPGPRGLVGPPGSRGNPGSRGENGPTGAVGFAGPQGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPHGPHGVPGLKGGRGTQGPPGATGFPGSAGRVGPPGPAGAPGPAGPAGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGSPGDKGDPGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKVGPTGATGDKGPPGPVGPPGSNGPVGEPGPEGPAGNDGTPGRDGAVGERGDRGDPGPAGLPGSQGAPGTPGPVGAPGDAGQRGEPGSRGPVGPPGRAGKRGLPGPQGPRGDKGDNGDRGDRGQKGHRGFTGLQGLPGPPGPNGEQGSAGIPGPFGPRGPPGPVGPSGKEGNPGPLGPIGPPGVRGSVGEAGPEGPPGEPGPPGPPGPPGHLTAALGDIMGHYDENMPDPLPEFTEDQAAPDDTNKTDPGIHVTLKSLSSQIETMRSPDGSKKHPARTCDDLKLCHPTKQSGEYWIDPNQGSAEDAIKVYCNMETGETCISANPASVPRKTWWASKSPDNKPVWYGLDMNRGSQFTYGDYQSPNTAITQMTFLRLLSKEASQNLTYICRNTVGYMDDQAKNLKKAVVLKGSNDLEIKGEGNIRFRYTVLQDTCSKRNGNVGKTIFEYRTQNVARLPIIDVGPVDIGNADQEFGLDIGPVCFM

Target ORF information:

RefSeq Version	NM_007737.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type V, alpha 2 (Col5a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007737.2

ORF Insert Sequence:

ATGATGGCAA ACTGGGTGGG AGCAAGACCT CTCCTCATTC TTTCTGTTTT ATTAGGGTAC 
TGTGTCTCAA TAAAAGCCCA GGAACAAGAG AATGATGAAT ATGATGAAGA AATAGCCTGT 
ACTCAACATG GACAGATGTA TTTAAATAGA GACATTTGGA AACCTTCTCC ATGTCAGATC 
TGTGTCTGTG ACAATGGTGC CATTCTTTGT GACAAGATAG AGTGCCCAGA GGTGCTCAAC 
TGTGCCAATC CTATAACACC CACTGGGGAG TGCTGTCCGG TGTGCCCACA AACTGGAGGT 
GGAGATACTA GTTTTGGTAG AGGAAGAAAG GGACAAAAAG GAGAACCAGG ATTAGTGCCT 
GTTGTAACAG GTATACGCGG TCGTCCAGGA CCTGCAGGCC CCCCAGGATC ACAAGGACCA 
AGAGGGGACC GAGGCCCTAA AGGAAGACCT GGTCCTCGTG GTCCTCAGGG AATTGATGGA 
GAGCCAGGTG TGCCTGGTCA GCCAGGTGCT CCAGGGCCTC CTGGGCACCC CTCTCACCCA 
GGACCTGATG GCATGAGCAG GCCTTTTTCA GCTCAGATGG CTGGGCTGGA TGAAAAATCT 
GGACTTGGAA GTCAAGTAGG ACTCATGCCT GGCTCTGTGG GTCCTGTTGG CCCAAGAGGA 
CCTCAGGGTT TACAAGGACA GCAAGGTGGT GTGGGACCCG CAGGACCTCC TGGTGAGCCT 
GGCGAGCCTG GACCAATGGG TCCAATTGGT TCTCGTGGAC CAGAGGGCCC ACCTGGGAAG 
CCCGGAGAAG ATGGTGAACC TGGAAGAAAT GGGAATACAG GTGAAGTGGG ATTCTCAGGA 
TCACCAGGTG CTCGAGGCTT CCCTGGTGCT CCTGGTCTCC CTGGCTTGAA AGGTCACAGA 
GGACACAAAG GTCTTGAAGG CCCCAAAGGT GAAATTGGAG CACCTGGTGC TAAGGGTGAA 
GCTGGCCCCA CTGGTCCAAT GGGTGCTATG GGCCCTCTGG GACCAAGAGG AATGCCAGGA 
GAGAGGGGAA GACTTGGGCC ACAGGGTGCT CCTGGAAAAC GGGGTGCCCA CGGTATGCCT 
GGAAAACCTG GACCAATGGG TCCTCTTGGG ATACCTGGCT CTTCCGGTTT TCCAGGAAAT 
CCTGGAATGA AGGGAGAAGC AGGTCCTACT GGAGCCCGAG GCCCTGAAGG TCCTCAGGGG 
CAGAGAGGTG AAACTGGGCC ACCTGGTCCA GCTGGCTCTC AGGGTCTTCC TGGTGCAGTG 
GGGACTGATG GTACACCTGG CGCCAAAGGC CCCACAGGCT CTGCAGGAAC CTCTGGCCCT 
CCTGGCTTGG CAGGGCCCCC TGGGTCTCCT GGCCCTCAAG GCAGTACTGG ACCTCAGGGA 
ATCCGAGGAC AATCGGGTGA TCCAGGAGTT CCAGGTTTCA AAGGAGAAGC TGGTCCAAAG 
GGAGAACCAG GGCCACATGG TATTCAGGGT CCCATTGGTC CACCTGGTGA AGAAGGCAAA 
AGAGGTCCTC GGGGTGATCC AGGCACAGTT GGTCCTCCAG GACCAATGGG AGAAAGGGGC 
GCTCCTGGCA ATCGTGGTTT TCCAGGGTCT GATGGTTTAC CTGGACCAAA GGGTGCTCAG 
GGAGAGCGTG GTCCTGTAGG TTCTTCGGGG CCTAAAGGAG GCCAGGGAGA TCCAGGACGT 
CCAGGAGAAC CTGGGCTTCC AGGTGCTCGG GGTCTGACAG GAAATCCTGG TGTTCAAGGT 
CCTGAAGGAA AACTTGGGCC TTTGGGTGCT CCTGGGGAAG ATGGTCGGCC AGGTCCTCCA 
GGCTCCATTG GGATCAGAGG ACAGCCTGGA AGCATGGGCC TTCCAGGCCC CAAAGGTAGC 
AGTGGTGACC TTGGAAAACC AGGAGAAGCA GGAAATGCTG GTGTGCCTGG GCAAAGGGGG 
GCTCCTGGCA AAGATGGAGA AGTTGGTCCT TCAGGCCCTG TGGGACCTCC GGGCCTAGCT 
GGGGAAAGAG GAGAGCAGGG ACCTCCAGGC CCCACTGGCT TTCAGGGACT TCCTGGACCT 
CCAGGGCCAC CTGGAGAAGG TGGAAAGGCT GGTGATCAAG GTGTTCCTGG AGAGCCTGGA 
GCAGTTGGCC CATTAGGACC TCGGGGAGAA AGAGGGAATC CTGGAGAAAG AGGAGAGCCA 
GGGATAACTG GACTGCCTGG GGAGAAAGGC ATGGCTGGAG GACATGGCCC TGATGGTCCA 
AAGGGCAACC CAGGTCCAAC TGGGACCATT GGAGACACAG GGCCACCAGG TCTTCAGGGC 
ATGCCAGGAG AAAGAGGAAT TGCAGGAACA CCTGGCCCTA AGGGTGACCG AGGTGGCATA 
GGAGAGAAAG GTGCTGAAGG AACAGCAGGA AATGACGGGG CAAGGGGTCT TCCAGGTCCC 
TTGGGACCTC CAGGTCCTGC AGGCCCTACT GGAGAAAAGG GTGAACCTGG TCCTAGAGGC 
TTAGTTGGCC CTCCTGGCTC CCGTGGCAAT CCTGGTTCTC GTGGTGAAAA TGGTCCAACT 
GGAGCTGTTG GTTTTGCTGG ACCCCAGGGT CCCGATGGAC AGCCTGGTGT CAAGGGTGAG 
CCTGGAGAGC CAGGACAGAA GGGAGATGCT GGGTCTCCTG GACCCCAAGG GCTAGCAGGA 
TCACCTGGCC CCCATGGTCC ACATGGTGTT CCTGGACTGA AAGGCGGTAG AGGAACCCAG 
GGTCCACCTG GTGCTACAGG GTTTCCTGGT TCTGCTGGCA GAGTTGGGCC TCCAGGCCCT 
GCTGGAGCTC CAGGACCTGC AGGGCCCGCA GGAGAACCTG GGAAGGAAGG ACCTCCTGGT 
CTTCGTGGTG ACCCTGGCTC TCATGGGCGA GTGGGAGATA GAGGACCAGC TGGCCCACCT 
GGTAGCCCAG GAGATAAAGG GGACCCAGGA GAAGATGGAC AACCTGGTCC AGATGGTCCT 
CCTGGTCCCG CTGGTACCAC TGGGCAAAGA GGAATTGTTG GAATGCCCGG ACAACGTGGG 
GAACGAGGAA TGCCTGGCCT TCCTGGCCCA GCGGGTACAC CAGGAAAAGT AGGACCAACT 
GGTGCGACAG GAGATAAAGG TCCCCCTGGA CCTGTAGGAC CTCCAGGTTC CAATGGTCCT 
GTAGGAGAAC CTGGGCCAGA AGGTCCGGCT GGTAATGATG GTACCCCAGG ACGGGATGGT 
GCTGTTGGAG AACGGGGTGA CCGTGGAGAC CCCGGGCCTG CTGGTCTACC AGGCTCTCAG 
GGTGCCCCTG GAACTCCTGG CCCTGTTGGT GCCCCTGGAG ATGCAGGACA AAGAGGAGAG 
CCAGGTTCTC GAGGCCCTGT AGGACCACCT GGTCGAGCTG GAAAGCGTGG TTTACCTGGA 
CCACAAGGAC CTCGAGGTGA CAAGGGTGAC AATGGAGACA GAGGTGACCG AGGTCAGAAA 
GGCCACAGAG GGTTCACTGG CCTGCAGGGT CTTCCTGGAC CTCCTGGTCC AAATGGTGAA 
CAAGGAAGTG CTGGCATTCC TGGTCCTTTC GGTCCAAGAG GTCCTCCAGG ACCAGTAGGT 
CCTTCAGGAA AGGAAGGAAA CCCTGGGCCA CTTGGACCAA TTGGACCTCC TGGTGTGCGG 
GGCAGTGTAG GAGAAGCAGG ACCAGAGGGT CCTCCTGGTG AACCTGGGCC TCCTGGCCCT 
CCAGGTCCCC CTGGCCATCT TACTGCTGCT CTTGGTGATA TCATGGGACA CTATGATGAG 
AACATGCCAG ATCCACTTCC AGAGTTTACG GAAGACCAGG CAGCTCCAGA TGACACAAAC 
AAAACAGACC CTGGGATCCA TGTTACCCTG AAGTCTCTCA GTAGTCAGAT TGAAACCATG 
CGTAGCCCTG ATGGTTCCAA GAAACACCCA GCCCGCACTT GTGATGATTT AAAGCTTTGC 
CATCCCACAA AGCAGAGCGG TGAATATTGG ATTGATCCTA ACCAGGGATC AGCTGAAGAT 
GCAATCAAAG TTTACTGCAA TATGGAAACA GGAGAGACAT GTATTTCAGC AAACCCAGCC 
AGTGTTCCAC GTAAAACCTG GTGGGCCAGC AAATCCCCTG ACAATAAGCC TGTGTGGTAC 
GGTCTTGACA TGAATAGAGG ATCTCAGTTC ACATATGGAG ATTACCAGTC TCCTAACACA 
GCTATCACTC AGATGACCTT TTTGCGTCTT TTATCAAAAG AAGCCTCGCA GAACCTTACT 
TACATCTGTA GGAACACTGT TGGATATATG GATGATCAAG CTAAAAACCT CAAGAAAGCT 
GTGGTCCTTA AAGGGTCAAA CGACTTAGAA ATAAAAGGAG AAGGAAACAT CAGATTCAGA 
TACACAGTTC TTCAAGACAC CTGCTCTAAG CGAAATGGAA ATGTAGGCAA AACTATCTTT 
GAATACAGAA CACAGAATGT GGCCCGCTTG CCCATCATAG ATGTTGGTCC TGTGGATATT 
GGCAATGCAG ACCAGGAATT TGGCCTTGAT ATTGGGCCAG TTTGTTTCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

How aging impacts skin biomechanics: a multiscale study in mice.
Scientific reports7(1)13750(2017 Oct)
Lynch B,Bonod-Bidaud C,Ducourthial G,Affagard JS,Bancelin S,Psilodimitrakopoulos S,Ruggiero F,Allain JM,Schanne-Klein MC

Deficits in Col5a2 Expression Result in Novel Skin and Adipose Abnormalities and Predisposition to Aortic Aneurysms and Dissections.
The American journal of pathology187(10)2300-2311(2017 Oct)
Park AC,Phan N,Massoudi D,Liu Z,Kernien JF,Adams SM,Davidson JM,Birk DE,Liu B,Greenspan DS

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

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Col5a2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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