COL4A6 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_006724617.3	XP_006724680.1	collagen alpha-6(IV) chain isoform X1
NM_033641.4	NP_378667.1	collagen alpha-6(IV) chain isoform B precursor
XM_011530853.2	XP_011529155.1	collagen alpha-6(IV) chain isoform X3
NM_033641.3	NP_378667.1	collagen alpha-6(IV) chain isoform B precursor
XM_011530853.3	XP_011529155.1	collagen alpha-6(IV) chain isoform X3
NM_001287760.1	NP_001274689.1	collagen alpha-6(IV) chain isoform 5 precursor
NM_001287758.1	NP_001274687.1	collagen alpha-6(IV) chain isoform 3 precursor
NM_001847.3	NP_001838.2	collagen alpha-6(IV) chain isoform A precursor
NM_001287759.1	NP_001274688.1	collagen alpha-6(IV) chain isoform 4 precursor
NM_001847.4	NP_001838.2	collagen alpha-6(IV) chain isoform A precursor
XM_011530852.2	XP_011529154.1	collagen alpha-6(IV) chain isoform X2
XM_011530854.2	XP_011529156.1	collagen alpha-6(IV) chain isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00921	NM_001287760.1 Latest version!	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu01044	NM_001287758.1 Latest version!	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu01057	NM_033641.4 Latest version!	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu01135	NM_001287759.1 Latest version!	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu08685	NM_001847.4 Latest version!	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu54120	XM_006724617.3 Latest version!	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu56982	XM_011530852.2 Latest version!	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu56983	XM_011530853.3 Latest version!	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu56984	XM_011530854.2 Latest version!	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OHu01057	NM_033641.3	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu56983	XM_011530853.2	Homo sapiens collagen type IV alpha 6 (COL4A6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu08685	NM_001847.3	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
Hide Old Accession Versions >>

COL4A6 ( NM_001287760.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274689.1 Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu00921
Clone ID Related Accession (Same CDS sequence)	NM_001287760.1
Accession Version	NM_001287760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Homo sapiens(human)
Product	collagen alpha-6(IV) chain isoform 5 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL034369.1, BC000874.1, BC140855.1, U04845.1, AL136080.6, DB260304.1 and CB050016.1. Transcript Variant: This variant (5) lacks several alternate in-frame exons compared to variant 3. The resulting protein (isoform 5) is shorter compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC140855.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACCGAGGA ACTGGCAGCA 
GCGGGAGAGA AGTCTTATGG AAAGCCATGT GGGGGCCAGG ACTGCAGTGG GAGCTGTCAG 
TGTTTTCCTG AGAAAGGAGC GAGAGGACGA CCTGGACCAA TTGGAATTCA AGGCCCAACA 
GGTCCTCAAG GATTCACTGG CTCTACTGGT TTATCGGGAT TGAAAGGAGA AAGGGGTTTC 
CCAGGCCTTC TGGGACCTTA TGGACCAAAA GGAGATAAGG GTCCCATGGG AGTTCCTGGC 
TTTCTTGGCA TCAATGGGAT TCCGGGCCAC CCTGGACAAC CAGGCCCCAG AGGCCCACCT 
GGTCTGGATG GCTGTAATGG AACTCAAGGA GCTGTTGGAT TTCCAGGCCC TGATGGCTAT 
CCTGGGCTTC TCGGACCACC CGGGCTTCCT GGTCAGAAAG GATCAAAAGG TGACCCTGTC 
CTTGCTCCAG GTAGTTTCAA AGGAATGAAG GGGGATCCTG GGCTGCCTGG ACTGGATGGA 
ATCACTGGCC CACAAGGAGC ACCCGGATTT CCTGGAGCTG TAGGACCTGC AGGACCACCA 
GGATTACAAG GTCCTCCAGG GCCTCCTGGT CCTCTTGGTC CTGATGGGAA TATGGGGCTA 
GGTTTTCAAG GAGAGAAAGG AGTCAAGGGG GATGTTGGCC TCCCTGGCCC AGCAGGACCT 
CCACCATCTA CTGGAGAGCT GGAATTCATG GGATTCCCCA AAGGGAAGAA AGGATCCAAG 
GGTGAACCAG GGCCTAAGGG TTTTCCAGGC ATAAGTGGCC CTCCAGGCTT CCCGGGCCTT 
GGAACTACTG GAGAAAAGGG AGAAAAGGGA GAAAAGGGAA TCCCTGGTTT GCCAGGACCT 
AGGGGTCCCA TGGGTTCAGA AGGAGTCCAA GGCCCTCCAG GGCAACAGGG CAAGAAAGGG 
ACCCTGGGAT TTCCTGGGCT TAATGGATTC CAAGGAATTG AGGGTCAAAA GGGTGACATT 
GGCCTGCCAG GCCCAGATGT TTTCATCGAT ATAGATGGTG CTGTGATCTC AGGTAATCCT 
GGAGATCCTG GTGTACCTGG CCTCCCAGGC CTTAAAGGAG ATGAAGGCAT CCAAGGCCTA 
CGTGGCCCTT CTGGTGTCCC TGGATTGCCA GCATTATCAG GTGTCCCAGG AGCCCTAGGG 
CCTCAGGGAT TTCCAGGGCT GAAGGGGGAC CAAGGAAACC CAGGCCGTAC CACAATTGGA 
GCAGCTGGCC TCCCTGGCAG AGATGGTTTG CCAGGCCCAC CAGGTCCACC AGGCCCACCT 
AGTCCAGAAT TTGAGACTGA AACTCTACAC AACAAAGAGT CAGGGTTCCC TGGTCTCCGA 
GGAGAACAAG GTCCAAAAGG AAACCTAGGC CTCAAAGGAA TAAAAGGAGA CTCAGGTTTC 
TGTGCTTGTG ACGGTGGTGT TCCCAACACT GGACCACCCG GGGAACCAGG CCCACCTGGT 
CCATGGGGTC TCATAGGCCT TCCAGGCCTT AAAGGAGCCA GAGGAGATCG AGGCTCTGGG 
GGTGCACAGG GCCCAGCAGG GGCTCCAGGC TTAGTTGGGC CTCTGGGTCC TTCAGGACCC 
AAAGGAAAGA AGGGGGAACC AATTCTCAGT ACAATCCAAG GAATGCCAGG AGATCGGGGT 
GATTCTGGCT CCCAGGGCTT CCGTGGTGTA ATAGGAGAAC CAGGCAAGGA CGGAGTACCA 
GGTTTACCAG GTCTGCCAGG CCTTCCGGGT GATGGTGGAC AGGGCTTCCC AGGTGAAAAG 
GGGTTACCTG GACTTCCTGG TGAAAAAGGC CATCCTGGTC CACCTGGCCT CCCAGGAAAT 
GGGTTACCAG GACTTCCTGG ACCCCGTGGG CTTCCTGGAG ATAAAGGCAA GGATGGATTA 
CCGGGACAAC AAGGCCTTCC CGGATCTAAG GGAATCACCC TGCCCTGTAT TATTCCTGGG 
TCATACGGTC CATCAGGATT TCCAGGCACT CCCGGATTCC CAGGCCCTAA AGGGTCTCGA 
GGCCTCCCTG GGACCCCAGG CCAGCCTGGG TCAAGTGGAA GTAAAGGAGA GCCAGGGAGT 
CCAGGATTGG TTCATCTTCC TGAATTACCA GGATTTCCTG GACCTCGTGG GGAGAAGGGC 
TTGCCTGGGT TTCCTGGGCT CCCTGGAAAA GATGGCTTGC CTGGGATGAT TGGCAGTCCA 
GGCTTACCTG GTTCCAAGGG AGCCACTGGT GACATCTTTG GTGCTGAAAA TGGTGCTCCG 
GGGGAACAAG GCCTACAAGG ATTAACAGGG CACAAAGGAT TTCTTGGAGA CTCTGGCCTT 
CCAGGACTCA AGGGTGTGCA CGGGAAGCCT GGCTTACTAG GCCCCAAAGG TGAGCGGGGC 
AGCCCTGGGA CACCAGGACA GGTGGGACAG CCAGGCACCC CAGGATCTAG TGGTCCATAT 
GGCATCAAGG GCAAATCTGG GCTCCCAGGA GCACCAGGCT TCCCAGGCAT CTCAGGACAT 
CCTGGAAAGA AAGGAACAAG AGGCAAGAAA GGTCCTCCTG GATCAATTGT AAAGAAAGGG 
CTGCCAGGGC TAAAAGGCCT TCCTGGAAAT CCAGGCCTAG TAGGACTGAA AGGAAGCCCA 
GGCTCTCCAG GGGTCGCTGG GTTGCCAGCC CTCTCTGGAC CCAAGGGAGA GAAGGGGTCT 
GTTGGATTCG TAGGTTTTCC AGGAATACCA GGTCTGCCTG GTATTCCTGG AACAAGAGGA 
TTAAAAGGAA TTCCAGGATC AACTGGAAAA ATGGGACCAT CTGGACGTGC TGGTACTCCT 
GGTGAAAAGG GAGACAGAGG CAATCCGGGG CCAGTCGGAA TACCTAGTCC AAGACGTCCA 
ATGTCAAACC TTTGGCTCAA AGGAGACAAA GGCTCTCAAG GCTCAGCCGG ATCCAATGGA 
TTTCCTGGGC CAAGAGGTGA CAAAGGAGAG GCTGGTCGAC CTGGACCACC AGGCCTACCT 
GGAGCTCCTG GCCTCCCAGG CATTATCAAA GGAGTTAGTG GAAAGCCAGG GCCCCCTGGC 
TTCATGGGAA TCCGGGGCTT ACCTGGCCTG AAGGGGTCCT CTGGGATCAC AGGTTTCCCA 
GGAATGCCAG GAGAAAGTGG TTCACAAGGT ATCAGAGGGT CGCCTGGACT CCCAGGAGCA 
TCTGGTCTCC CAGGCCTGAA AGGAGACAAC GGCCAGACAG TTGAAATTTC CGGTAGCCCA 
GGACCCAAGG GACAGCCTGG CGAATCTGGT TTTAAAGGCA CAAAAGGAAG AGATGGACTA 
ATAGGCAATA TAGGCTTCCC TGGAAACAAA GGTGAAGATG GAAAAGTTGG TGTTTCTGGA 
GATGTTGGCC TTCCTGGAGC TCCAGGATTT CCAGGAGTTG CCGGCATGAG AGGAGAACCA 
GGACTTCCAG GTTCTTCTGG TCACCAAGGG GCAATTGGGC CTCTAGGATC CCCCGGATTA 
ATAGGACCCA AAGGACCTAG TATCACCGGT GTGCCTGGGC CTGCTGGTCT CCCTGGACCC 
AAAGGAGAAA AAGGATATCC AGGAATTGGC ATCGGAGCTC CAGGGAAGCC GGGCCTGAGA 
GGGCAAAAAG GTGATCGAGG TTTCCCAGGT CTCCAGGGCC CTGCTGGTCT CCCCGGTGCC 
CCAGGCATCT CCTTGCCCTC ACTCATAGCA GGACAGCCTG GTGACCCCGG GCGACCAGGC 
CTAGATGGAG AACGAGGCCG CCCAGGCCCC GCTGGACCCC CAGGTCCCCC TGGGCCATCC 
TCGAATCAAG GCGACACCGG AGACCCTGGC TTCCCTGGAA TTCCTGGACC TAAAGGGCCT 
AAGGGAGACC AAGGAATTCC AGGTTTTTCT GGCCTCCCTG GAGAGCTAGG ACTGAAAGGC 
TCTTCTGGCC TCCAAGGTGA TCCTGGACAA ACACCAACTG CAGAAGCTGT CCAGGTTCCT 
CCTGGACCCT TGGGTCTACC AGGGATCGAT GGCATCCCTG GCCTCACTGG GGACCCTGGG 
GCTCAAGGCC CTGTAGGCCT ACAAGGCTCC AAAGGTTTAC CTGGCATCCC CGGTAAAGAT 
GGCCCCAGTG GGCTCCCAGG CCCACCTGGG GCTCTTGGTG ATCCTGGTCT GCCTGGACTG 
CAAGGCCCTC CAGGATTTGA AGGAGCTCCA GGGCAGCAAG GCCCCTTCGG GATGCCTGGA 
ATGCCTGGCC AGAGCATGAG AGTGGGCTAC ACGTTGGTAA AGCACAGCCA GTCGGAACAG 
GTGCCCCCGT GTCCCATCGG GATGAGCCAG CTGTGGGTGG GGTACAGCTT ACTGTTTGTG 
GAGGGGCAAG AGAAAGCCCA CAACCAGGAC CTGGGCTTTG CTGGCTCCTG TCTGCCCCGC 
TTCAGCACCA TGCCCTTCAT CTACTGCAAC ATCAACGAGG TGTGCCACTA TGCCAGGCGC 
AATGATAAAT CTTACTGGCT CTCCACTACC GCCCCTATCC CCATGATGCC CGTCAGCCAG 
ACCCAGATTC CCCAGTACAT CAGCCGCTGC TCTGTGTGTG AGGCACCCTC GCAAGCCATT 
GCTGTGCACA GCCAGGACAT CACCATCCCG CAGTGCCCCC TGGGCTGGCG CAGCCTCTGG 
ATTGGGTACT CTTTCCTCAT GCACACTGCC GCTGGTGCCG AGGGTGGAGG CCAGTCCCTG 
GTCTCACCTG GCTCCTGCCT AGAGGACTTT CGGGCCACTC CTTTCATCGA ATGCAGTGGT 
GCCCGAGGCA CCTGCCACTA CTTTGCAAAC AAGTACAGTT TCTGGTTGAC CACAGTGGAG 
GAGAGGCAGC AGTTTGGGGA GTTGCCTGTG TCTGAAACGC TGAAAGCTGG GCAGCTCCAC 
ACTCGAGTCA GTCGCTGCCA GGTGTGTATG AAAAGCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274689.1
CDS	235..5136
Translation	MHPGLWLLLVTLCLTEELAAAGEKSYGKPCGGQDCSGSCQCFPEKGARGRPGPIGIQGPTGPQGFTGSTGLSGLKGERGFPGLLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYPGLLGPPGLPGQKGSKGDPVLAPGSFKGMKGDPGLPGLDGITGPQGAPGFPGAVGPAGPPGLQGPPGPPGPLGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPKGFPGISGPPGFPGLGTTGEKGEKGEKGIPGLPGPRGPMGSEGVQGPPGQQGKKGTLGFPGLNGFQGIEGQKGDIGLPGPDVFIDIDGAVISGNPGDPGVPGLPGLKGDEGIQGLRGPSGVPGLPALSGVPGALGPQGFPGLKGDQGNPGRTTIGAAGLPGRDGLPGPPGPPGPPSPEFETETLHNKESGFPGLRGEQGPKGNLGLKGIKGDSGFCACDGGVPNTGPPGEPGPPGPWGLIGLPGLKGARGDRGSGGAQGPAGAPGLVGPLGPSGPKGKKGEPILSTIQGMPGDRGDSGSQGFRGVIGEPGKDGVPGLPGLPGLPGDGGQGFPGEKGLPGLPGEKGHPGPPGLPGNGLPGLPGPRGLPGDKGKDGLPGQQGLPGSKGITLPCIIPGSYGPSGFPGTPGFPGPKGSRGLPGTPGQPGSSGSKGEPGSPGLVHLPELPGFPGPRGEKGLPGFPGLPGKDGLPGMIGSPGLPGSKGATGDIFGAENGAPGEQGLQGLTGHKGFLGDSGLPGLKGVHGKPGLLGPKGERGSPGTPGQVGQPGTPGSSGPYGIKGKSGLPGAPGFPGISGHPGKKGTRGKKGPPGSIVKKGLPGLKGLPGNPGLVGLKGSPGSPGVAGLPALSGPKGEKGSVGFVGFPGIPGLPGIPGTRGLKGIPGSTGKMGPSGRAGTPGEKGDRGNPGPVGIPSPRRPMSNLWLKGDKGSQGSAGSNGFPGPRGDKGEAGRPGPPGLPGAPGLPGIIKGVSGKPGPPGFMGIRGLPGLKGSSGITGFPGMPGESGSQGIRGSPGLPGASGLPGLKGDNGQTVEISGSPGPKGQPGESGFKGTKGRDGLIGNIGFPGNKGEDGKVGVSGDVGLPGAPGFPGVAGMRGEPGLPGSSGHQGAIGPLGSPGLIGPKGPSITGVPGPAGLPGPKGEKGYPGIGIGAPGKPGLRGQKGDRGFPGLQGPAGLPGAPGISLPSLIAGQPGDPGRPGLDGERGRPGPAGPPGPPGPSSNQGDTGDPGFPGIPGPKGPKGDQGIPGFSGLPGELGLKGSSGLQGDPGQTPTAEAVQVPPGPLGLPGIDGIPGLTGDPGAQGPVGLQGSKGLPGIPGKDGPSGLPGPPGALGDPGLPGLQGPPGFEGAPGQQGPFGMPGMPGQSMRVGYTLVKHSQSEQVPPCPIGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVSQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGELPVSETLKAGQLHTRVSRCQVCMKSL

Target ORF information:

RefSeq Version	NM_001287760.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287760.1

ORF Insert Sequence:

ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACCGAGGA ACTGGCAGCA 
GCGGGAGAGA AGTCTTATGG AAAGCCATGT GGGGGCCAGG ACTGCAGTGG GAGCTGTCAG 
TGTTTTCCTG AGAAAGGAGC GAGAGGACGA CCTGGACCAA TTGGAATTCA AGGCCCAACA 
GGTCCTCAAG GATTCACTGG CTCTACTGGT TTATCGGGAT TGAAAGGAGA AAGGGGTTTC 
CCAGGCCTTC TGGGACCTTA TGGACCAAAA GGAGATAAGG GTCCCATGGG AGTTCCTGGC 
TTTCTTGGCA TCAATGGGAT TCCGGGCCAC CCTGGACAAC CAGGCCCCAG AGGCCCACCT 
GGTCTGGATG GCTGTAATGG AACTCAAGGA GCTGTTGGAT TTCCAGGCCC TGATGGCTAT 
CCTGGGCTTC TCGGACCACC CGGGCTTCCT GGTCAGAAAG GATCAAAAGG TGACCCTGTC 
CTTGCTCCAG GTAGTTTCAA AGGAATGAAG GGGGATCCTG GGCTGCCTGG ACTGGATGGA 
ATCACTGGCC CACAAGGAGC ACCCGGATTT CCTGGAGCTG TAGGACCTGC AGGACCACCA 
GGATTACAAG GTCCTCCAGG GCCTCCTGGT CCTCTTGGTC CTGATGGGAA TATGGGGCTA 
GGTTTTCAAG GAGAGAAAGG AGTCAAGGGG GATGTTGGCC TCCCTGGCCC AGCAGGACCT 
CCACCATCTA CTGGAGAGCT GGAATTCATG GGATTCCCCA AAGGGAAGAA AGGATCCAAG 
GGTGAACCAG GGCCTAAGGG TTTTCCAGGC ATAAGTGGCC CTCCAGGCTT CCCGGGCCTT 
GGAACTACTG GAGAAAAGGG AGAAAAGGGA GAAAAGGGAA TCCCTGGTTT GCCAGGACCT 
AGGGGTCCCA TGGGTTCAGA AGGAGTCCAA GGCCCTCCAG GGCAACAGGG CAAGAAAGGG 
ACCCTGGGAT TTCCTGGGCT TAATGGATTC CAAGGAATTG AGGGTCAAAA GGGTGACATT 
GGCCTGCCAG GCCCAGATGT TTTCATCGAT ATAGATGGTG CTGTGATCTC AGGTAATCCT 
GGAGATCCTG GTGTACCTGG CCTCCCAGGC CTTAAAGGAG ATGAAGGCAT CCAAGGCCTA 
CGTGGCCCTT CTGGTGTCCC TGGATTGCCA GCATTATCAG GTGTCCCAGG AGCCCTAGGG 
CCTCAGGGAT TTCCAGGGCT GAAGGGGGAC CAAGGAAACC CAGGCCGTAC CACAATTGGA 
GCAGCTGGCC TCCCTGGCAG AGATGGTTTG CCAGGCCCAC CAGGTCCACC AGGCCCACCT 
AGTCCAGAAT TTGAGACTGA AACTCTACAC AACAAAGAGT CAGGGTTCCC TGGTCTCCGA 
GGAGAACAAG GTCCAAAAGG AAACCTAGGC CTCAAAGGAA TAAAAGGAGA CTCAGGTTTC 
TGTGCTTGTG ACGGTGGTGT TCCCAACACT GGACCACCCG GGGAACCAGG CCCACCTGGT 
CCATGGGGTC TCATAGGCCT TCCAGGCCTT AAAGGAGCCA GAGGAGATCG AGGCTCTGGG 
GGTGCACAGG GCCCAGCAGG GGCTCCAGGC TTAGTTGGGC CTCTGGGTCC TTCAGGACCC 
AAAGGAAAGA AGGGGGAACC AATTCTCAGT ACAATCCAAG GAATGCCAGG AGATCGGGGT 
GATTCTGGCT CCCAGGGCTT CCGTGGTGTA ATAGGAGAAC CAGGCAAGGA CGGAGTACCA 
GGTTTACCAG GTCTGCCAGG CCTTCCGGGT GATGGTGGAC AGGGCTTCCC AGGTGAAAAG 
GGGTTACCTG GACTTCCTGG TGAAAAAGGC CATCCTGGTC CACCTGGCCT CCCAGGAAAT 
GGGTTACCAG GACTTCCTGG ACCCCGTGGG CTTCCTGGAG ATAAAGGCAA GGATGGATTA 
CCGGGACAAC AAGGCCTTCC CGGATCTAAG GGAATCACCC TGCCCTGTAT TATTCCTGGG 
TCATACGGTC CATCAGGATT TCCAGGCACT CCCGGATTCC CAGGCCCTAA AGGGTCTCGA 
GGCCTCCCTG GGACCCCAGG CCAGCCTGGG TCAAGTGGAA GTAAAGGAGA GCCAGGGAGT 
CCAGGATTGG TTCATCTTCC TGAATTACCA GGATTTCCTG GACCTCGTGG GGAGAAGGGC 
TTGCCTGGGT TTCCTGGGCT CCCTGGAAAA GATGGCTTGC CTGGGATGAT TGGCAGTCCA 
GGCTTACCTG GTTCCAAGGG AGCCACTGGT GACATCTTTG GTGCTGAAAA TGGTGCTCCG 
GGGGAACAAG GCCTACAAGG ATTAACAGGG CACAAAGGAT TTCTTGGAGA CTCTGGCCTT 
CCAGGACTCA AGGGTGTGCA CGGGAAGCCT GGCTTACTAG GCCCCAAAGG TGAGCGGGGC 
AGCCCTGGGA CACCAGGACA GGTGGGACAG CCAGGCACCC CAGGATCTAG TGGTCCATAT 
GGCATCAAGG GCAAATCTGG GCTCCCAGGA GCACCAGGCT TCCCAGGCAT CTCAGGACAT 
CCTGGAAAGA AAGGAACAAG AGGCAAGAAA GGTCCTCCTG GATCAATTGT AAAGAAAGGG 
CTGCCAGGGC TAAAAGGCCT TCCTGGAAAT CCAGGCCTAG TAGGACTGAA AGGAAGCCCA 
GGCTCTCCAG GGGTCGCTGG GTTGCCAGCC CTCTCTGGAC CCAAGGGAGA GAAGGGGTCT 
GTTGGATTCG TAGGTTTTCC AGGAATACCA GGTCTGCCTG GTATTCCTGG AACAAGAGGA 
TTAAAAGGAA TTCCAGGATC AACTGGAAAA ATGGGACCAT CTGGACGTGC TGGTACTCCT 
GGTGAAAAGG GAGACAGAGG CAATCCGGGG CCAGTCGGAA TACCTAGTCC AAGACGTCCA 
ATGTCAAACC TTTGGCTCAA AGGAGACAAA GGCTCTCAAG GCTCAGCCGG ATCCAATGGA 
TTTCCTGGGC CAAGAGGTGA CAAAGGAGAG GCTGGTCGAC CTGGACCACC AGGCCTACCT 
GGAGCTCCTG GCCTCCCAGG CATTATCAAA GGAGTTAGTG GAAAGCCAGG GCCCCCTGGC 
TTCATGGGAA TCCGGGGCTT ACCTGGCCTG AAGGGGTCCT CTGGGATCAC AGGTTTCCCA 
GGAATGCCAG GAGAAAGTGG TTCACAAGGT ATCAGAGGGT CGCCTGGACT CCCAGGAGCA 
TCTGGTCTCC CAGGCCTGAA AGGAGACAAC GGCCAGACAG TTGAAATTTC CGGTAGCCCA 
GGACCCAAGG GACAGCCTGG CGAATCTGGT TTTAAAGGCA CAAAAGGAAG AGATGGACTA 
ATAGGCAATA TAGGCTTCCC TGGAAACAAA GGTGAAGATG GAAAAGTTGG TGTTTCTGGA 
GATGTTGGCC TTCCTGGAGC TCCAGGATTT CCAGGAGTTG CCGGCATGAG AGGAGAACCA 
GGACTTCCAG GTTCTTCTGG TCACCAAGGG GCAATTGGGC CTCTAGGATC CCCCGGATTA 
ATAGGACCCA AAGGACCTAG TATCACCGGT GTGCCTGGGC CTGCTGGTCT CCCTGGACCC 
AAAGGAGAAA AAGGATATCC AGGAATTGGC ATCGGAGCTC CAGGGAAGCC GGGCCTGAGA 
GGGCAAAAAG GTGATCGAGG TTTCCCAGGT CTCCAGGGCC CTGCTGGTCT CCCCGGTGCC 
CCAGGCATCT CCTTGCCCTC ACTCATAGCA GGACAGCCTG GTGACCCCGG GCGACCAGGC 
CTAGATGGAG AACGAGGCCG CCCAGGCCCC GCTGGACCCC CAGGTCCCCC TGGGCCATCC 
TCGAATCAAG GCGACACCGG AGACCCTGGC TTCCCTGGAA TTCCTGGACC TAAAGGGCCT 
AAGGGAGACC AAGGAATTCC AGGTTTTTCT GGCCTCCCTG GAGAGCTAGG ACTGAAAGGC 
TCTTCTGGCC TCCAAGGTGA TCCTGGACAA ACACCAACTG CAGAAGCTGT CCAGGTTCCT 
CCTGGACCCT TGGGTCTACC AGGGATCGAT GGCATCCCTG GCCTCACTGG GGACCCTGGG 
GCTCAAGGCC CTGTAGGCCT ACAAGGCTCC AAAGGTTTAC CTGGCATCCC CGGTAAAGAT 
GGCCCCAGTG GGCTCCCAGG CCCACCTGGG GCTCTTGGTG ATCCTGGTCT GCCTGGACTG 
CAAGGCCCTC CAGGATTTGA AGGAGCTCCA GGGCAGCAAG GCCCCTTCGG GATGCCTGGA 
ATGCCTGGCC AGAGCATGAG AGTGGGCTAC ACGTTGGTAA AGCACAGCCA GTCGGAACAG 
GTGCCCCCGT GTCCCATCGG GATGAGCCAG CTGTGGGTGG GGTACAGCTT ACTGTTTGTG 
GAGGGGCAAG AGAAAGCCCA CAACCAGGAC CTGGGCTTTG CTGGCTCCTG TCTGCCCCGC 
TTCAGCACCA TGCCCTTCAT CTACTGCAAC ATCAACGAGG TGTGCCACTA TGCCAGGCGC 
AATGATAAAT CTTACTGGCT CTCCACTACC GCCCCTATCC CCATGATGCC CGTCAGCCAG 
ACCCAGATTC CCCAGTACAT CAGCCGCTGC TCTGTGTGTG AGGCACCCTC GCAAGCCATT 
GCTGTGCACA GCCAGGACAT CACCATCCCG CAGTGCCCCC TGGGCTGGCG CAGCCTCTGG 
ATTGGGTACT CTTTCCTCAT GCACACTGCC GCTGGTGCCG AGGGTGGAGG CCAGTCCCTG 
GTCTCACCTG GCTCCTGCCT AGAGGACTTT CGGGCCACTC CTTTCATCGA ATGCAGTGGT 
GCCCGAGGCA CCTGCCACTA CTTTGCAAAC AAGTACAGTT TCTGGTTGAC CACAGTGGAG 
GAGAGGCAGC AGTTTGGGGA GTTGCCTGTG TCTGAAACGC TGAAAGCTGG GCAGCTCCAC 
ACTCGAGTCA GTCGCTGCCA GGTGTGTATG AAAAGCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( NM_001287758.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274687.1 Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu01044
Clone ID Related Accession (Same CDS sequence)	NM_001287758.1
Accession Version	NM_001287758.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Homo sapiens(human)
Product	collagen alpha-6(IV) chain isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL034369.1, CN313347.1, AW082083.1, AL136080.6, CN313341.1, CN313332.1, CN313351.1, CA396616.1, CA489342.1, CN313343.1, CX872164.1, DA748513.1, BG680578.1, AL031177.1 and CB050016.1. On Dec 19, 2013 this sequence version replaced XM_005262073.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest protein (isoform 3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2146982 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACCGAGGA ACTGGCAGCA 
GCGGGAGAGA AGTCTTATGG AAAGCCATGT GGGGGCCAGG ACTGCAGTGG GAGCTGTCAG 
TGTTTTCCTG AGAAAGGAGC GAGAGGACGA CCTGGACCAA TTGGAATTCA AGGCCCAACA 
GGTCCTCAAG GATTCACTGG CTCTACTGGT TTATCGGGAT TGAAAGGAGA AAGGGGTTTC 
CCAGGCCTTC TGGGACCTTA TGGACCAAAA GGAGATAAGG GTCCCATGGG AGTTCCTGGC 
TTTCTTGGCA TCAATGGGAT TCCGGGCCAC CCTGGACAAC CAGGCCCCAG AGGCCCACCT 
GGTCTGGATG GCTGTAATGG AACTCAAGGA GCTGTTGGAT TTCCAGGCCC TGATGGCTAT 
CCTGGGCTTC TCGGACCACC CGGGCTTCCT GGTCAGAAAG GATCAAAAGG TGACCCTGTC 
CTTGCTCCAG GTAGTTTCAA AGGAATGAAG GGGGATCCTG GGCTGCCTGG ACTGGATGGA 
ATCACTGGCC CACAAGGAGC ACCCGGATTT CCTGGAGCTG TAGGACCTGC AGGACCACCA 
GGATTACAAG GTCCTCCAGG GCCTCCTGGT CCTCTTGGTC CTGATGGGAA TATGGGGCTA 
GGTTTTCAAG GAGAGAAAGG AGTCAAGGGG GATGTTGGCC TCCCTGGCCC AGCAGGACCT 
CCACCATCTA CTGGAGAGCT GGAATTCATG GGATTCCCCA AAGGGAAGAA AGGATCCAAG 
GGTGAACCAG GGCCTAAGGG TTTTCCAGGC ATAAGTGGCC CTCCAGGCTT CCCGGGCCTT 
GGAACTACTG GAGAAAAGGG AGAAAAGGGA GAAAAGGGAA TCCCTGGTTT GCCAGGACCT 
AGGGGTCCCA TGGGTTCAGA AGGAGTCCAA GGCCCTCCAG GGCAACAGGG CAAGAAAGGG 
ACCCTGGGAT TTCCTGGGCT TAATGGATTC CAAGGAATTG AGGGTCAAAA GGGTGACATT 
GGCCTGCCAG GCCCAGATGT TTTCATCGAT ATAGATGGTG CTGTGATCTC AGGTAATCCT 
GGAGATCCTG GTGTACCTGG CCTCCCAGGC CTTAAAGGAG ATGAAGGCAT CCAAGGCCTA 
CGTGGCCCTT CTGGTGTCCC TGGATTGCCA GCATTATCAG GTGTCCCAGG AGCCCTAGGG 
CCTCAGGGAT TTCCAGGGCT GAAGGGGGAC CAAGGAAACC CAGGCCGTAC CACAATTGGA 
GCAGCTGGCC TCCCTGGCAG AGATGGTTTG CCAGGCCCAC CAGGTCCACC AGGCCCACCT 
AGTCCAGAAT TTGAGACTGA AACTCTACAC AACAAAGAGT CAGGGTTCCC TGGTCTCCGA 
GGAGAACAAG GTCCAAAAGG AAACCTAGGC CTCAAAGGAA TAAAAGGAGA CTCAGGTTTC 
TGTGCTTGTG ACGGTGGTGT TCCCAACACT GGACCACCCG GGGAACCAGG CCCACCTGGT 
CCATGGGGTC TCATAGGCCT TCCAGGCCTT AAAGGAGCCA GAGGAGATCG AGGCTCTGGG 
GGTGCACAGG GCCCAGCAGG GGCTCCAGGC TTAGTTGGGC CTCTGGGTCC TTCAGGACCC 
AAAGGAAAGA AGGGGGAACC AATTCTCAGT ACAATCCAAG GAATGCCAGG AGATCGGGGT 
GATTCTGGCT CCCAGGGCTT CCGTGGTGTA ATAGGAGAAC CAGGCAAGGA CGGAGTACCA 
GGTTTACCAG GTCTGCCAGG CCTTCCGGGT GATGGTGGAC AGGGCTTCCC AGGTGAAAAG 
GGGTTACCTG GACTTCCTGG TGAAAAAGGC CATCCTGGTC CACCTGGCCT CCCAGGAAAT 
GGGTTACCAG GACTTCCTGG ACCCCGTGGG CTTCCTGGAG ATAAAGGCAA GGATGGATTA 
CCGGGACAAC AAGGCCTTCC CGGATCTAAG GGTGACTGCT GCTGTAGAGA GGTTGGTAAA 
GGAGACTTAG ACACAGAGAG AGGAATCACC CTGCCCTGTA TTATTCCTGG GTCATACGGT 
CCATCAGGAT TTCCAGGCAC TCCCGGATTC CCAGGCCCTA AAGGGTCTCG AGGCCTCCCT 
GGGACCCCAG GCCAGCCTGG GTCAAGTGGA AGTAAAGGAG AGCCAGGGAG TCCAGGATTG 
GTTCATCTTC CTGAATTACC AGGATTTCCT GGACCTCGTG GGGAGAAGGG CTTGCCTGGG 
TTTCCTGGGC TCCCTGGAAA AGATGGCTTG CCTGGGATGA TTGGCAGTCC AGGCTTACCT 
GGTTCCAAGG GAGCCACTGG TGACATCTTT GGTGCTGAAA ATGGTGCTCC GGGGGAACAA 
GGCCTACAAG GATTAACAGG GCACAAAGGA TTTCTTGGAG ACTCTGGCCT TCCAGGACTC 
AAGGGTGTGC ACGGGAAGCC TGGCTTACTA GGCCCCAAAG GTGAGCGGGG CAGCCCTGGG 
ACACCAGGAC AGGTGGGACA GCCAGGCACC CCAGGATCTA GTGGTCCATA TGGCATCAAG 
GGCAAATCTG GGCTCCCAGG AGCACCAGGC TTCCCAGGCA TCTCAGGACA TCCTGGAAAG 
AAAGGAACAA GAGGCAAGAA AGGTCCTCCT GGATCAATTG TAAAGAAAGG GCTGCCAGGG 
CTAAAAGGCC TTCCTGGAAA TCCAGGCCTA GTAGGACTGA AAGGAAGCCC AGGCTCTCCA 
GGGGTCGCTG GGTTGCCAGC CCTCTCTGGA CCCAAGGGAG AGAAGGGGTC TGTTGGATTC 
GTAGGTTTTC CAGGAATACC AGGTCTGCCT GGTATTCCTG GAACAAGAGG ATTAAAAGGA 
ATTCCAGGAT CAACTGGAAA AATGGGACCA TCTGGACGTG CTGGTACTCC TGGTGAAAAG 
GGAGACAGAG GCAATCCGGG GCCAGTCGGA ATACCTAGTC CAAGACGTCC AATGTCAAAC 
CTTTGGCTCA AAGGAGACAA AGGCTCTCAA GGCTCAGCCG GATCCAATGG ATTTCCTGGG 
CCAAGAGGTG ACAAAGGAGA GGCTGGTCGA CCTGGACCAC CAGGCCTACC TGGAGCTCCT 
GGCCTCCCAG GCATTATCAA AGGAGTTAGT GGAAAGCCAG GGCCCCCTGG CTTCATGGGA 
ATCCGGGGCT TACCTGGCCT GAAGGGGTCC TCTGGGATCA CAGGTTTCCC AGGAATGCCA 
GGAGAAAGTG GTTCACAAGG TATCAGAGGG TCGCCTGGAC TCCCAGGAGC ATCTGGTCTC 
CCAGGCCTGA AAGGAGACAA CGGCCAGACA GTTGAAATTT CCGGTAGCCC AGGACCCAAG 
GGACAGCCTG GCGAATCTGG TTTTAAAGGC ACAAAAGGAA GAGATGGACT AATAGGCAAT 
ATAGGCTTCC CTGGAAACAA AGGTGAAGAT GGAAAAGTTG GTGTTTCTGG AGATGTTGGC 
CTTCCTGGAG CTCCAGGATT TCCAGGAGTT GCCGGCATGA GAGGAGAACC AGGACTTCCA 
GGTTCTTCTG GTCACCAAGG GGCAATTGGG CCTCTAGGAT CCCCCGGATT AATAGGACCC 
AAAGGCTTCC CTGGATTTCC TGGTTTACAT GGACTGAATG GGCTTCCGGG CACCAAGGGT 
ACCCATGGCA CTCCAGGACC TAGTATCACC GGTGTGCCTG GGCCTGCTGG TCTCCCTGGA 
CCCAAAGGAG AAAAAGGATA TCCAGGAATT GGCATCGGAG CTCCAGGGAA GCCGGGCCTG 
AGAGGGCAAA AAGGTGATCG AGGTTTCCCA GGTCTCCAGG GCCCTGCTGG TCTCCCCGGT 
GCCCCAGGCA TCTCCTTGCC CTCACTCATA GCAGGACAGC CTGGTGACCC CGGGCGACCA 
GGCCTAGATG GAGAACGAGG CCGCCCAGGC CCCGCTGGAC CCCCAGGTCC CCCTGGGCCA 
TCCTCGAATC AAGGCGACAC CGGAGACCCT GGCTTCCCTG GAATTCCTGG ACCTAAAGGG 
CCTAAGGGAG ACCAAGGAAT TCCAGGTTTT TCTGGCCTCC CTGGAGAGCT AGGACTGAAA 
GGCATGAGAG GTGAGCCTGG CTTCATGGGG ACTCCAGGCA AGGTTGGGCC ACCTGGAGAC 
CCAGGATTTC CCGGAATGAA GGGGAAGGCA GGGCCAAGAG GCTCTTCTGG CCTCCAAGGT 
GATCCTGGAC AAACACCAAC TGCAGAAGCT GTCCAGGTTC CTCCTGGACC CTTGGGTCTA 
CCAGGGATCG ATGGCATCCC TGGCCTCACT GGGGACCCTG GGGCTCAAGG CCCTGTAGGC 
CTACAAGGCT CCAAAGGTTT ACCTGGCATC CCCGGTAAAG ATGGCCCCAG TGGGCTCCCA 
GGCCCACCTG GGGCTCTTGG TGATCCTGGT CTGCCTGGAC TGCAAGGCCC TCCAGGATTT 
GAAGGAGCTC CAGGGCAGCA AGGCCCCTTC GGGATGCCTG GAATGCCTGG CCAGAGCATG 
AGAGTGGGCT ACACGTTGGT AAAGCACAGC CAGTCGGAAC AGGTGCCCCC GTGTCCCATC 
GGGATGAGCC AGCTGTGGGT GGGGTACAGC TTACTGTTTG TGGAGGGGCA AGAGAAAGCC 
CACAACCAGG ACCTGGGCTT TGCTGGCTCC TGTCTGCCCC GCTTCAGCAC CATGCCCTTC 
ATCTACTGCA ACATCAACGA GGTGTGCCAC TATGCCAGGC GCAATGATAA ATCTTACTGG 
CTCTCCACTA CCGCCCCTAT CCCCATGATG CCCGTCAGCC AGACCCAGAT TCCCCAGTAC 
ATCAGCCGCT GCTCTGTGTG TGAGGCACCC TCGCAAGCCA TTGCTGTGCA CAGCCAGGAC 
ATCACCATCC CGCAGTGCCC CCTGGGCTGG CGCAGCCTCT GGATTGGGTA CTCTTTCCTC 
ATGCACACTG CCGCTGGTGC CGAGGGTGGA GGCCAGTCCC TGGTCTCACC TGGCTCCTGC 
CTAGAGGACT TTCGGGCCAC TCCTTTCATC GAATGCAGTG GTGCCCGAGG CACCTGCCAC 
TACTTTGCAA ACAAGTACAG TTTCTGGTTG ACCACAGTGG AGGAGAGGCA GCAGTTTGGG 
GAGTTGCCTG TGTCTGAAAC GCTGAAAGCT GGGCAGCTCC ACACTCGAGT CAGTCGCTGC 
CAGGTGTGTA TGAAAAGCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274687.1
CDS	235..5358
Translation	MHPGLWLLLVTLCLTEELAAAGEKSYGKPCGGQDCSGSCQCFPEKGARGRPGPIGIQGPTGPQGFTGSTGLSGLKGERGFPGLLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYPGLLGPPGLPGQKGSKGDPVLAPGSFKGMKGDPGLPGLDGITGPQGAPGFPGAVGPAGPPGLQGPPGPPGPLGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPKGFPGISGPPGFPGLGTTGEKGEKGEKGIPGLPGPRGPMGSEGVQGPPGQQGKKGTLGFPGLNGFQGIEGQKGDIGLPGPDVFIDIDGAVISGNPGDPGVPGLPGLKGDEGIQGLRGPSGVPGLPALSGVPGALGPQGFPGLKGDQGNPGRTTIGAAGLPGRDGLPGPPGPPGPPSPEFETETLHNKESGFPGLRGEQGPKGNLGLKGIKGDSGFCACDGGVPNTGPPGEPGPPGPWGLIGLPGLKGARGDRGSGGAQGPAGAPGLVGPLGPSGPKGKKGEPILSTIQGMPGDRGDSGSQGFRGVIGEPGKDGVPGLPGLPGLPGDGGQGFPGEKGLPGLPGEKGHPGPPGLPGNGLPGLPGPRGLPGDKGKDGLPGQQGLPGSKGDCCCREVGKGDLDTERGITLPCIIPGSYGPSGFPGTPGFPGPKGSRGLPGTPGQPGSSGSKGEPGSPGLVHLPELPGFPGPRGEKGLPGFPGLPGKDGLPGMIGSPGLPGSKGATGDIFGAENGAPGEQGLQGLTGHKGFLGDSGLPGLKGVHGKPGLLGPKGERGSPGTPGQVGQPGTPGSSGPYGIKGKSGLPGAPGFPGISGHPGKKGTRGKKGPPGSIVKKGLPGLKGLPGNPGLVGLKGSPGSPGVAGLPALSGPKGEKGSVGFVGFPGIPGLPGIPGTRGLKGIPGSTGKMGPSGRAGTPGEKGDRGNPGPVGIPSPRRPMSNLWLKGDKGSQGSAGSNGFPGPRGDKGEAGRPGPPGLPGAPGLPGIIKGVSGKPGPPGFMGIRGLPGLKGSSGITGFPGMPGESGSQGIRGSPGLPGASGLPGLKGDNGQTVEISGSPGPKGQPGESGFKGTKGRDGLIGNIGFPGNKGEDGKVGVSGDVGLPGAPGFPGVAGMRGEPGLPGSSGHQGAIGPLGSPGLIGPKGFPGFPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGYPGIGIGAPGKPGLRGQKGDRGFPGLQGPAGLPGAPGISLPSLIAGQPGDPGRPGLDGERGRPGPAGPPGPPGPSSNQGDTGDPGFPGIPGPKGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGFPGMKGKAGPRGSSGLQGDPGQTPTAEAVQVPPGPLGLPGIDGIPGLTGDPGAQGPVGLQGSKGLPGIPGKDGPSGLPGPPGALGDPGLPGLQGPPGFEGAPGQQGPFGMPGMPGQSMRVGYTLVKHSQSEQVPPCPIGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVSQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGELPVSETLKAGQLHTRVSRCQVCMKSL

Target ORF information:

RefSeq Version	NM_001287758.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287758.1

ORF Insert Sequence:

ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACCGAGGA ACTGGCAGCA 
GCGGGAGAGA AGTCTTATGG AAAGCCATGT GGGGGCCAGG ACTGCAGTGG GAGCTGTCAG 
TGTTTTCCTG AGAAAGGAGC GAGAGGACGA CCTGGACCAA TTGGAATTCA AGGCCCAACA 
GGTCCTCAAG GATTCACTGG CTCTACTGGT TTATCGGGAT TGAAAGGAGA AAGGGGTTTC 
CCAGGCCTTC TGGGACCTTA TGGACCAAAA GGAGATAAGG GTCCCATGGG AGTTCCTGGC 
TTTCTTGGCA TCAATGGGAT TCCGGGCCAC CCTGGACAAC CAGGCCCCAG AGGCCCACCT 
GGTCTGGATG GCTGTAATGG AACTCAAGGA GCTGTTGGAT TTCCAGGCCC TGATGGCTAT 
CCTGGGCTTC TCGGACCACC CGGGCTTCCT GGTCAGAAAG GATCAAAAGG TGACCCTGTC 
CTTGCTCCAG GTAGTTTCAA AGGAATGAAG GGGGATCCTG GGCTGCCTGG ACTGGATGGA 
ATCACTGGCC CACAAGGAGC ACCCGGATTT CCTGGAGCTG TAGGACCTGC AGGACCACCA 
GGATTACAAG GTCCTCCAGG GCCTCCTGGT CCTCTTGGTC CTGATGGGAA TATGGGGCTA 
GGTTTTCAAG GAGAGAAAGG AGTCAAGGGG GATGTTGGCC TCCCTGGCCC AGCAGGACCT 
CCACCATCTA CTGGAGAGCT GGAATTCATG GGATTCCCCA AAGGGAAGAA AGGATCCAAG 
GGTGAACCAG GGCCTAAGGG TTTTCCAGGC ATAAGTGGCC CTCCAGGCTT CCCGGGCCTT 
GGAACTACTG GAGAAAAGGG AGAAAAGGGA GAAAAGGGAA TCCCTGGTTT GCCAGGACCT 
AGGGGTCCCA TGGGTTCAGA AGGAGTCCAA GGCCCTCCAG GGCAACAGGG CAAGAAAGGG 
ACCCTGGGAT TTCCTGGGCT TAATGGATTC CAAGGAATTG AGGGTCAAAA GGGTGACATT 
GGCCTGCCAG GCCCAGATGT TTTCATCGAT ATAGATGGTG CTGTGATCTC AGGTAATCCT 
GGAGATCCTG GTGTACCTGG CCTCCCAGGC CTTAAAGGAG ATGAAGGCAT CCAAGGCCTA 
CGTGGCCCTT CTGGTGTCCC TGGATTGCCA GCATTATCAG GTGTCCCAGG AGCCCTAGGG 
CCTCAGGGAT TTCCAGGGCT GAAGGGGGAC CAAGGAAACC CAGGCCGTAC CACAATTGGA 
GCAGCTGGCC TCCCTGGCAG AGATGGTTTG CCAGGCCCAC CAGGTCCACC AGGCCCACCT 
AGTCCAGAAT TTGAGACTGA AACTCTACAC AACAAAGAGT CAGGGTTCCC TGGTCTCCGA 
GGAGAACAAG GTCCAAAAGG AAACCTAGGC CTCAAAGGAA TAAAAGGAGA CTCAGGTTTC 
TGTGCTTGTG ACGGTGGTGT TCCCAACACT GGACCACCCG GGGAACCAGG CCCACCTGGT 
CCATGGGGTC TCATAGGCCT TCCAGGCCTT AAAGGAGCCA GAGGAGATCG AGGCTCTGGG 
GGTGCACAGG GCCCAGCAGG GGCTCCAGGC TTAGTTGGGC CTCTGGGTCC TTCAGGACCC 
AAAGGAAAGA AGGGGGAACC AATTCTCAGT ACAATCCAAG GAATGCCAGG AGATCGGGGT 
GATTCTGGCT CCCAGGGCTT CCGTGGTGTA ATAGGAGAAC CAGGCAAGGA CGGAGTACCA 
GGTTTACCAG GTCTGCCAGG CCTTCCGGGT GATGGTGGAC AGGGCTTCCC AGGTGAAAAG 
GGGTTACCTG GACTTCCTGG TGAAAAAGGC CATCCTGGTC CACCTGGCCT CCCAGGAAAT 
GGGTTACCAG GACTTCCTGG ACCCCGTGGG CTTCCTGGAG ATAAAGGCAA GGATGGATTA 
CCGGGACAAC AAGGCCTTCC CGGATCTAAG GGTGACTGCT GCTGTAGAGA GGTTGGTAAA 
GGAGACTTAG ACACAGAGAG AGGAATCACC CTGCCCTGTA TTATTCCTGG GTCATACGGT 
CCATCAGGAT TTCCAGGCAC TCCCGGATTC CCAGGCCCTA AAGGGTCTCG AGGCCTCCCT 
GGGACCCCAG GCCAGCCTGG GTCAAGTGGA AGTAAAGGAG AGCCAGGGAG TCCAGGATTG 
GTTCATCTTC CTGAATTACC AGGATTTCCT GGACCTCGTG GGGAGAAGGG CTTGCCTGGG 
TTTCCTGGGC TCCCTGGAAA AGATGGCTTG CCTGGGATGA TTGGCAGTCC AGGCTTACCT 
GGTTCCAAGG GAGCCACTGG TGACATCTTT GGTGCTGAAA ATGGTGCTCC GGGGGAACAA 
GGCCTACAAG GATTAACAGG GCACAAAGGA TTTCTTGGAG ACTCTGGCCT TCCAGGACTC 
AAGGGTGTGC ACGGGAAGCC TGGCTTACTA GGCCCCAAAG GTGAGCGGGG CAGCCCTGGG 
ACACCAGGAC AGGTGGGACA GCCAGGCACC CCAGGATCTA GTGGTCCATA TGGCATCAAG 
GGCAAATCTG GGCTCCCAGG AGCACCAGGC TTCCCAGGCA TCTCAGGACA TCCTGGAAAG 
AAAGGAACAA GAGGCAAGAA AGGTCCTCCT GGATCAATTG TAAAGAAAGG GCTGCCAGGG 
CTAAAAGGCC TTCCTGGAAA TCCAGGCCTA GTAGGACTGA AAGGAAGCCC AGGCTCTCCA 
GGGGTCGCTG GGTTGCCAGC CCTCTCTGGA CCCAAGGGAG AGAAGGGGTC TGTTGGATTC 
GTAGGTTTTC CAGGAATACC AGGTCTGCCT GGTATTCCTG GAACAAGAGG ATTAAAAGGA 
ATTCCAGGAT CAACTGGAAA AATGGGACCA TCTGGACGTG CTGGTACTCC TGGTGAAAAG 
GGAGACAGAG GCAATCCGGG GCCAGTCGGA ATACCTAGTC CAAGACGTCC AATGTCAAAC 
CTTTGGCTCA AAGGAGACAA AGGCTCTCAA GGCTCAGCCG GATCCAATGG ATTTCCTGGG 
CCAAGAGGTG ACAAAGGAGA GGCTGGTCGA CCTGGACCAC CAGGCCTACC TGGAGCTCCT 
GGCCTCCCAG GCATTATCAA AGGAGTTAGT GGAAAGCCAG GGCCCCCTGG CTTCATGGGA 
ATCCGGGGCT TACCTGGCCT GAAGGGGTCC TCTGGGATCA CAGGTTTCCC AGGAATGCCA 
GGAGAAAGTG GTTCACAAGG TATCAGAGGG TCGCCTGGAC TCCCAGGAGC ATCTGGTCTC 
CCAGGCCTGA AAGGAGACAA CGGCCAGACA GTTGAAATTT CCGGTAGCCC AGGACCCAAG 
GGACAGCCTG GCGAATCTGG TTTTAAAGGC ACAAAAGGAA GAGATGGACT AATAGGCAAT 
ATAGGCTTCC CTGGAAACAA AGGTGAAGAT GGAAAAGTTG GTGTTTCTGG AGATGTTGGC 
CTTCCTGGAG CTCCAGGATT TCCAGGAGTT GCCGGCATGA GAGGAGAACC AGGACTTCCA 
GGTTCTTCTG GTCACCAAGG GGCAATTGGG CCTCTAGGAT CCCCCGGATT AATAGGACCC 
AAAGGCTTCC CTGGATTTCC TGGTTTACAT GGACTGAATG GGCTTCCGGG CACCAAGGGT 
ACCCATGGCA CTCCAGGACC TAGTATCACC GGTGTGCCTG GGCCTGCTGG TCTCCCTGGA 
CCCAAAGGAG AAAAAGGATA TCCAGGAATT GGCATCGGAG CTCCAGGGAA GCCGGGCCTG 
AGAGGGCAAA AAGGTGATCG AGGTTTCCCA GGTCTCCAGG GCCCTGCTGG TCTCCCCGGT 
GCCCCAGGCA TCTCCTTGCC CTCACTCATA GCAGGACAGC CTGGTGACCC CGGGCGACCA 
GGCCTAGATG GAGAACGAGG CCGCCCAGGC CCCGCTGGAC CCCCAGGTCC CCCTGGGCCA 
TCCTCGAATC AAGGCGACAC CGGAGACCCT GGCTTCCCTG GAATTCCTGG ACCTAAAGGG 
CCTAAGGGAG ACCAAGGAAT TCCAGGTTTT TCTGGCCTCC CTGGAGAGCT AGGACTGAAA 
GGCATGAGAG GTGAGCCTGG CTTCATGGGG ACTCCAGGCA AGGTTGGGCC ACCTGGAGAC 
CCAGGATTTC CCGGAATGAA GGGGAAGGCA GGGCCAAGAG GCTCTTCTGG CCTCCAAGGT 
GATCCTGGAC AAACACCAAC TGCAGAAGCT GTCCAGGTTC CTCCTGGACC CTTGGGTCTA 
CCAGGGATCG ATGGCATCCC TGGCCTCACT GGGGACCCTG GGGCTCAAGG CCCTGTAGGC 
CTACAAGGCT CCAAAGGTTT ACCTGGCATC CCCGGTAAAG ATGGCCCCAG TGGGCTCCCA 
GGCCCACCTG GGGCTCTTGG TGATCCTGGT CTGCCTGGAC TGCAAGGCCC TCCAGGATTT 
GAAGGAGCTC CAGGGCAGCA AGGCCCCTTC GGGATGCCTG GAATGCCTGG CCAGAGCATG 
AGAGTGGGCT ACACGTTGGT AAAGCACAGC CAGTCGGAAC AGGTGCCCCC GTGTCCCATC 
GGGATGAGCC AGCTGTGGGT GGGGTACAGC TTACTGTTTG TGGAGGGGCA AGAGAAAGCC 
CACAACCAGG ACCTGGGCTT TGCTGGCTCC TGTCTGCCCC GCTTCAGCAC CATGCCCTTC 
ATCTACTGCA ACATCAACGA GGTGTGCCAC TATGCCAGGC GCAATGATAA ATCTTACTGG 
CTCTCCACTA CCGCCCCTAT CCCCATGATG CCCGTCAGCC AGACCCAGAT TCCCCAGTAC 
ATCAGCCGCT GCTCTGTGTG TGAGGCACCC TCGCAAGCCA TTGCTGTGCA CAGCCAGGAC 
ATCACCATCC CGCAGTGCCC CCTGGGCTGG CGCAGCCTCT GGATTGGGTA CTCTTTCCTC 
ATGCACACTG CCGCTGGTGC CGAGGGTGGA GGCCAGTCCC TGGTCTCACC TGGCTCCTGC 
CTAGAGGACT TTCGGGCCAC TCCTTTCATC GAATGCAGTG GTGCCCGAGG CACCTGCCAC 
TACTTTGCAA ACAAGTACAG TTTCTGGTTG ACCACAGTGG AGGAGAGGCA GCAGTTTGGG 
GAGTTGCCTG TGTCTGAAAC GCTGAAAGCT GGGCAGCTCC ACACTCGAGT CAGTCGCTGC 
CAGGTGTGTA TGAAAAGCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( NM_033641.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_378667.1 Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant B, mRNA.

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CloneID	OHu01057
Clone ID Related Accession (Same CDS sequence)	NM_033641.4 , NM_033641.3
Accession Version	NM_033641.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Homo sapiens(human)
Product	collagen alpha-6(IV) chain isoform B precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN313347.1, D21337.1, AL136080.6, U04845.1, BF726518.1, BQ331638.1 and CB050016.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_033641.3. Transcript Variant: This variant (B) utilizes the same 5' exon as variant 3 (exon 1B) but lacks several alternate in-frame exons. The resulting protein (isoform B) is shorter compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2146982 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACCGAGGA ACTGGCAGCA 
GCGGGAGAGA AGTCTTATGG AAAGCCATGT GGGGGCCAGG ACTGCAGTGG GAGCTGTCAG 
TGTTTTCCTG AGAAAGGAGC GAGAGGACGA CCTGGACCAA TTGGAATTCA AGGCCCAACA 
GGTCCTCAAG GATTCACTGG CTCTACTGGT TTATCGGGAT TGAAAGGAGA AAGGGGTTTC 
CCAGGCCTTC TGGGACCTTA TGGACCAAAA GGAGATAAGG GTCCCATGGG AGTTCCTGGC 
TTTCTTGGCA TCAATGGGAT TCCGGGCCAC CCTGGACAAC CAGGCCCCAG AGGCCCACCT 
GGTCTGGATG GCTGTAATGG AACTCAAGGA GCTGTTGGAT TTCCAGGCCC TGATGGCTAT 
CCTGGGCTTC TCGGACCACC CGGGCTTCCT GGTCAGAAAG GATCAAAAGG TGACCCTGTC 
CTTGCTCCAG GTAGTTTCAA AGGAATGAAG GGGGATCCTG GGCTGCCTGG ACTGGATGGA 
ATCACTGGCC CACAAGGAGC ACCCGGATTT CCTGGAGCTG TAGGACCTGC AGGACCACCA 
GGATTACAAG GTCCTCCAGG GCCTCCTGGT CCTCTTGGTC CTGATGGGAA TATGGGGCTA 
GGTTTTCAAG GAGAGAAAGG AGTCAAGGGG GATGTTGGCC TCCCTGGCCC AGCAGGACCT 
CCACCATCTA CTGGAGAGCT GGAATTCATG GGATTCCCCA AAGGGAAGAA AGGATCCAAG 
GGTGAACCAG GGCCTAAGGG TTTTCCAGGC ATAAGTGGCC CTCCAGGCTT CCCGGGCCTT 
GGAACTACTG GAGAAAAGGG AGAAAAGGGA GAAAAGGGAA TCCCTGGTTT GCCAGGACCT 
AGGGGTCCCA TGGGTTCAGA AGGAGTCCAA GGCCCTCCAG GGCAACAGGG CAAGAAAGGG 
ACCCTGGGAT TTCCTGGGCT TAATGGATTC CAAGGAATTG AGGGTCAAAA GGGTGACATT 
GGCCTGCCAG GCCCAGATGT TTTCATCGAT ATAGATGGTG CTGTGATCTC AGGTAATCCT 
GGAGATCCTG GTGTACCTGG CCTCCCAGGC CTTAAAGGAG ATGAAGGCAT CCAAGGCCTA 
CGTGGCCCTT CTGGTGTCCC TGGATTGCCA GCATTATCAG GTGTCCCAGG AGCCCTAGGG 
CCTCAGGGAT TTCCAGGGCT GAAGGGGGAC CAAGGAAACC CAGGCCGTAC CACAATTGGA 
GCAGCTGGCC TCCCTGGCAG AGATGGTTTG CCAGGCCCAC CAGGTCCACC AGGCCCACCT 
AGTCCAGAAT TTGAGACTGA AACTCTACAC AACAAAGAGT CAGGGTTCCC TGGTCTCCGA 
GGAGAACAAG GTCCAAAAGG AAACCTAGGC CTCAAAGGAA TAAAAGGAGA CTCAGGTTTC 
TGTGCTTGTG ACGGTGGTGT TCCCAACACT GGACCACCCG GGGAACCAGG CCCACCTGGT 
CCATGGGGTC TCATAGGCCT TCCAGGCCTT AAAGGAGCCA GAGGAGATCG AGGCTCTGGG 
GGTGCACAGG GCCCAGCAGG GGCTCCAGGC TTAGTTGGGC CTCTGGGTCC TTCAGGACCC 
AAAGGAAAGA AGGGGGAACC AATTCTCAGT ACAATCCAAG GAATGCCAGG AGATCGGGGT 
GATTCTGGCT CCCAGGGCTT CCGTGGTGTA ATAGGAGAAC CAGGCAAGGA CGGAGTACCA 
GGTTTACCAG GTCTGCCAGG CCTTCCGGGT GATGGTGGAC AGGGCTTCCC AGGTGAAAAG 
GGGTTACCTG GACTTCCTGG TGAAAAAGGC CATCCTGGTC CACCTGGCCT CCCAGGAAAT 
GGGTTACCAG GACTTCCTGG ACCCCGTGGG CTTCCTGGAG ATAAAGGCAA GGATGGATTA 
CCGGGACAAC AAGGCCTTCC CGGATCTAAG GGAATCACCC TGCCCTGTAT TATTCCTGGG 
TCATACGGTC CATCAGGATT TCCAGGCACT CCCGGATTCC CAGGCCCTAA AGGGTCTCGA 
GGCCTCCCTG GGACCCCAGG CCAGCCTGGG TCAAGTGGAA GTAAAGGAGA GCCAGGGAGT 
CCAGGATTGG TTCATCTTCC TGAATTACCA GGATTTCCTG GACCTCGTGG GGAGAAGGGC 
TTGCCTGGGT TTCCTGGGCT CCCTGGAAAA GATGGCTTGC CTGGGATGAT TGGCAGTCCA 
GGCTTACCTG GTTCCAAGGG AGCCACTGGT GACATCTTTG GTGCTGAAAA TGGTGCTCCG 
GGGGAACAAG GCCTACAAGG ATTAACAGGG CACAAAGGAT TTCTTGGAGA CTCTGGCCTT 
CCAGGACTCA AGGGTGTGCA CGGGAAGCCT GGCTTACTAG GCCCCAAAGG TGAGCGGGGC 
AGCCCTGGGA CACCAGGACA GGTGGGACAG CCAGGCACCC CAGGATCTAG TGGTCCATAT 
GGCATCAAGG GCAAATCTGG GCTCCCAGGA GCACCAGGCT TCCCAGGCAT CTCAGGACAT 
CCTGGAAAGA AAGGAACAAG AGGCAAGAAA GGTCCTCCTG GATCAATTGT AAAGAAAGGG 
CTGCCAGGGC TAAAAGGCCT TCCTGGAAAT CCAGGCCTAG TAGGACTGAA AGGAAGCCCA 
GGCTCTCCAG GGGTCGCTGG GTTGCCAGCC CTCTCTGGAC CCAAGGGAGA GAAGGGGTCT 
GTTGGATTCG TAGGTTTTCC AGGAATACCA GGTCTGCCTG GTATTCCTGG AACAAGAGGA 
TTAAAAGGAA TTCCAGGATC AACTGGAAAA ATGGGACCAT CTGGACGTGC TGGTACTCCT 
GGTGAAAAGG GAGACAGAGG CAATCCGGGG CCAGTCGGAA TACCTAGTCC AAGACGTCCA 
ATGTCAAACC TTTGGCTCAA AGGAGACAAA GGCTCTCAAG GCTCAGCCGG ATCCAATGGA 
TTTCCTGGGC CAAGAGGTGA CAAAGGAGAG GCTGGTCGAC CTGGACCACC AGGCCTACCT 
GGAGCTCCTG GCCTCCCAGG CATTATCAAA GGAGTTAGTG GAAAGCCAGG GCCCCCTGGC 
TTCATGGGAA TCCGGGGCTT ACCTGGCCTG AAGGGGTCCT CTGGGATCAC AGGTTTCCCA 
GGAATGCCAG GAGAAAGTGG TTCACAAGGT ATCAGAGGGT CGCCTGGACT CCCAGGAGCA 
TCTGGTCTCC CAGGCCTGAA AGGAGACAAC GGCCAGACAG TTGAAATTTC CGGTAGCCCA 
GGACCCAAGG GACAGCCTGG CGAATCTGGT TTTAAAGGCA CAAAAGGAAG AGATGGACTA 
ATAGGCAATA TAGGCTTCCC TGGAAACAAA GGTGAAGATG GAAAAGTTGG TGTTTCTGGA 
GATGTTGGCC TTCCTGGAGC TCCAGGATTT CCAGGAGTTG CCGGCATGAG AGGAGAACCA 
GGACTTCCAG GTTCTTCTGG TCACCAAGGG GCAATTGGGC CTCTAGGATC CCCCGGATTA 
ATAGGACCCA AAGGCTTCCC TGGATTTCCT GGTTTACATG GACTGAATGG GCTTCCGGGC 
ACCAAGGGTA CCCATGGCAC TCCAGGACCT AGTATCACCG GTGTGCCTGG GCCTGCTGGT 
CTCCCTGGAC CCAAAGGAGA AAAAGGATAT CCAGGAATTG GCATCGGAGC TCCAGGGAAG 
CCGGGCCTGA GAGGGCAAAA AGGTGATCGA GGTTTCCCAG GTCTCCAGGG CCCTGCTGGT 
CTCCCCGGTG CCCCAGGCAT CTCCTTGCCC TCACTCATAG CAGGACAGCC TGGTGACCCC 
GGGCGACCAG GCCTAGATGG AGAACGAGGC CGCCCAGGCC CCGCTGGACC CCCAGGTCCC 
CCTGGGCCAT CCTCGAATCA AGGCGACACC GGAGACCCTG GCTTCCCTGG AATTCCTGGA 
CCTAAAGGGC CTAAGGGAGA CCAAGGAATT CCAGGTTTTT CTGGCCTCCC TGGAGAGCTA 
GGACTGAAAG GCATGAGAGG TGAGCCTGGC TTCATGGGGA CTCCAGGCAA GGTTGGGCCA 
CCTGGAGACC CAGGATTTCC CGGAATGAAG GGGAAGGCAG GGCCAAGAGG CTCTTCTGGC 
CTCCAAGGTG ATCCTGGACA AACACCAACT GCAGAAGCTG TCCAGGTTCC TCCTGGACCC 
TTGGGTCTAC CAGGGATCGA TGGCATCCCT GGCCTCACTG GGGACCCTGG GGCTCAAGGC 
CCTGTAGGCC TACAAGGCTC CAAAGGTTTA CCTGGCATCC CCGGTAAAGA TGGCCCCAGT 
GGGCTCCCAG GCCCACCTGG GGCTCTTGGT GATCCTGGTC TGCCTGGACT GCAAGGCCCT 
CCAGGATTTG AAGGAGCTCC AGGGCAGCAA GGCCCCTTCG GGATGCCTGG AATGCCTGGC 
CAGAGCATGA GAGTGGGCTA CACGTTGGTA AAGCACAGCC AGTCGGAACA GGTGCCCCCG 
TGTCCCATCG GGATGAGCCA GCTGTGGGTG GGGTACAGCT TACTGTTTGT GGAGGGGCAA 
GAGAAAGCCC ACAACCAGGA CCTGGGCTTT GCTGGCTCCT GTCTGCCCCG CTTCAGCACC 
ATGCCCTTCA TCTACTGCAA CATCAACGAG GTGTGCCACT ATGCCAGGCG CAATGATAAA 
TCTTACTGGC TCTCCACTAC CGCCCCTATC CCCATGATGC CCGTCAGCCA GACCCAGATT 
CCCCAGTACA TCAGCCGCTG CTCTGTGTGT GAGGCACCCT CGCAAGCCAT TGCTGTGCAC 
AGCCAGGACA TCACCATCCC GCAGTGCCCC CTGGGCTGGC GCAGCCTCTG GATTGGGTAC 
TCTTTCCTCA TGCACACTGC CGCTGGTGCC GAGGGTGGAG GCCAGTCCCT GGTCTCACCT 
GGCTCCTGCC TAGAGGACTT TCGGGCCACT CCTTTCATCG AATGCAGTGG TGCCCGAGGC 
ACCTGCCACT ACTTTGCAAA CAAGTACAGT TTCTGGTTGA CCACAGTGGA GGAGAGGCAG 
CAGTTTGGGG AGTTGCCTGT GTCTGAAACG CTGAAAGCTG GGCAGCTCCA CACTCGAGTC 
AGTCGCTGCC AGGTGTGTAT GAAAAGCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_378667.1
CDS	227..5299
Translation	MHPGLWLLLVTLCLTEELAAAGEKSYGKPCGGQDCSGSCQCFPEKGARGRPGPIGIQGPTGPQGFTGSTGLSGLKGERGFPGLLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYPGLLGPPGLPGQKGSKGDPVLAPGSFKGMKGDPGLPGLDGITGPQGAPGFPGAVGPAGPPGLQGPPGPPGPLGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPKGFPGISGPPGFPGLGTTGEKGEKGEKGIPGLPGPRGPMGSEGVQGPPGQQGKKGTLGFPGLNGFQGIEGQKGDIGLPGPDVFIDIDGAVISGNPGDPGVPGLPGLKGDEGIQGLRGPSGVPGLPALSGVPGALGPQGFPGLKGDQGNPGRTTIGAAGLPGRDGLPGPPGPPGPPSPEFETETLHNKESGFPGLRGEQGPKGNLGLKGIKGDSGFCACDGGVPNTGPPGEPGPPGPWGLIGLPGLKGARGDRGSGGAQGPAGAPGLVGPLGPSGPKGKKGEPILSTIQGMPGDRGDSGSQGFRGVIGEPGKDGVPGLPGLPGLPGDGGQGFPGEKGLPGLPGEKGHPGPPGLPGNGLPGLPGPRGLPGDKGKDGLPGQQGLPGSKGITLPCIIPGSYGPSGFPGTPGFPGPKGSRGLPGTPGQPGSSGSKGEPGSPGLVHLPELPGFPGPRGEKGLPGFPGLPGKDGLPGMIGSPGLPGSKGATGDIFGAENGAPGEQGLQGLTGHKGFLGDSGLPGLKGVHGKPGLLGPKGERGSPGTPGQVGQPGTPGSSGPYGIKGKSGLPGAPGFPGISGHPGKKGTRGKKGPPGSIVKKGLPGLKGLPGNPGLVGLKGSPGSPGVAGLPALSGPKGEKGSVGFVGFPGIPGLPGIPGTRGLKGIPGSTGKMGPSGRAGTPGEKGDRGNPGPVGIPSPRRPMSNLWLKGDKGSQGSAGSNGFPGPRGDKGEAGRPGPPGLPGAPGLPGIIKGVSGKPGPPGFMGIRGLPGLKGSSGITGFPGMPGESGSQGIRGSPGLPGASGLPGLKGDNGQTVEISGSPGPKGQPGESGFKGTKGRDGLIGNIGFPGNKGEDGKVGVSGDVGLPGAPGFPGVAGMRGEPGLPGSSGHQGAIGPLGSPGLIGPKGFPGFPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGYPGIGIGAPGKPGLRGQKGDRGFPGLQGPAGLPGAPGISLPSLIAGQPGDPGRPGLDGERGRPGPAGPPGPPGPSSNQGDTGDPGFPGIPGPKGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGFPGMKGKAGPRGSSGLQGDPGQTPTAEAVQVPPGPLGLPGIDGIPGLTGDPGAQGPVGLQGSKGLPGIPGKDGPSGLPGPPGALGDPGLPGLQGPPGFEGAPGQQGPFGMPGMPGQSMRVGYTLVKHSQSEQVPPCPIGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVSQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGELPVSETLKAGQLHTRVSRCQVCMKSL

Target ORF information:

RefSeq Version	NM_033641.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033641.4

ORF Insert Sequence:

ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACCGAGGA ACTGGCAGCA 
GCGGGAGAGA AGTCTTATGG AAAGCCATGT GGGGGCCAGG ACTGCAGTGG GAGCTGTCAG 
TGTTTTCCTG AGAAAGGAGC GAGAGGACGA CCTGGACCAA TTGGAATTCA AGGCCCAACA 
GGTCCTCAAG GATTCACTGG CTCTACTGGT TTATCGGGAT TGAAAGGAGA AAGGGGTTTC 
CCAGGCCTTC TGGGACCTTA TGGACCAAAA GGAGATAAGG GTCCCATGGG AGTTCCTGGC 
TTTCTTGGCA TCAATGGGAT TCCGGGCCAC CCTGGACAAC CAGGCCCCAG AGGCCCACCT 
GGTCTGGATG GCTGTAATGG AACTCAAGGA GCTGTTGGAT TTCCAGGCCC TGATGGCTAT 
CCTGGGCTTC TCGGACCACC CGGGCTTCCT GGTCAGAAAG GATCAAAAGG TGACCCTGTC 
CTTGCTCCAG GTAGTTTCAA AGGAATGAAG GGGGATCCTG GGCTGCCTGG ACTGGATGGA 
ATCACTGGCC CACAAGGAGC ACCCGGATTT CCTGGAGCTG TAGGACCTGC AGGACCACCA 
GGATTACAAG GTCCTCCAGG GCCTCCTGGT CCTCTTGGTC CTGATGGGAA TATGGGGCTA 
GGTTTTCAAG GAGAGAAAGG AGTCAAGGGG GATGTTGGCC TCCCTGGCCC AGCAGGACCT 
CCACCATCTA CTGGAGAGCT GGAATTCATG GGATTCCCCA AAGGGAAGAA AGGATCCAAG 
GGTGAACCAG GGCCTAAGGG TTTTCCAGGC ATAAGTGGCC CTCCAGGCTT CCCGGGCCTT 
GGAACTACTG GAGAAAAGGG AGAAAAGGGA GAAAAGGGAA TCCCTGGTTT GCCAGGACCT 
AGGGGTCCCA TGGGTTCAGA AGGAGTCCAA GGCCCTCCAG GGCAACAGGG CAAGAAAGGG 
ACCCTGGGAT TTCCTGGGCT TAATGGATTC CAAGGAATTG AGGGTCAAAA GGGTGACATT 
GGCCTGCCAG GCCCAGATGT TTTCATCGAT ATAGATGGTG CTGTGATCTC AGGTAATCCT 
GGAGATCCTG GTGTACCTGG CCTCCCAGGC CTTAAAGGAG ATGAAGGCAT CCAAGGCCTA 
CGTGGCCCTT CTGGTGTCCC TGGATTGCCA GCATTATCAG GTGTCCCAGG AGCCCTAGGG 
CCTCAGGGAT TTCCAGGGCT GAAGGGGGAC CAAGGAAACC CAGGCCGTAC CACAATTGGA 
GCAGCTGGCC TCCCTGGCAG AGATGGTTTG CCAGGCCCAC CAGGTCCACC AGGCCCACCT 
AGTCCAGAAT TTGAGACTGA AACTCTACAC AACAAAGAGT CAGGGTTCCC TGGTCTCCGA 
GGAGAACAAG GTCCAAAAGG AAACCTAGGC CTCAAAGGAA TAAAAGGAGA CTCAGGTTTC 
TGTGCTTGTG ACGGTGGTGT TCCCAACACT GGACCACCCG GGGAACCAGG CCCACCTGGT 
CCATGGGGTC TCATAGGCCT TCCAGGCCTT AAAGGAGCCA GAGGAGATCG AGGCTCTGGG 
GGTGCACAGG GCCCAGCAGG GGCTCCAGGC TTAGTTGGGC CTCTGGGTCC TTCAGGACCC 
AAAGGAAAGA AGGGGGAACC AATTCTCAGT ACAATCCAAG GAATGCCAGG AGATCGGGGT 
GATTCTGGCT CCCAGGGCTT CCGTGGTGTA ATAGGAGAAC CAGGCAAGGA CGGAGTACCA 
GGTTTACCAG GTCTGCCAGG CCTTCCGGGT GATGGTGGAC AGGGCTTCCC AGGTGAAAAG 
GGGTTACCTG GACTTCCTGG TGAAAAAGGC CATCCTGGTC CACCTGGCCT CCCAGGAAAT 
GGGTTACCAG GACTTCCTGG ACCCCGTGGG CTTCCTGGAG ATAAAGGCAA GGATGGATTA 
CCGGGACAAC AAGGCCTTCC CGGATCTAAG GGAATCACCC TGCCCTGTAT TATTCCTGGG 
TCATACGGTC CATCAGGATT TCCAGGCACT CCCGGATTCC CAGGCCCTAA AGGGTCTCGA 
GGCCTCCCTG GGACCCCAGG CCAGCCTGGG TCAAGTGGAA GTAAAGGAGA GCCAGGGAGT 
CCAGGATTGG TTCATCTTCC TGAATTACCA GGATTTCCTG GACCTCGTGG GGAGAAGGGC 
TTGCCTGGGT TTCCTGGGCT CCCTGGAAAA GATGGCTTGC CTGGGATGAT TGGCAGTCCA 
GGCTTACCTG GTTCCAAGGG AGCCACTGGT GACATCTTTG GTGCTGAAAA TGGTGCTCCG 
GGGGAACAAG GCCTACAAGG ATTAACAGGG CACAAAGGAT TTCTTGGAGA CTCTGGCCTT 
CCAGGACTCA AGGGTGTGCA CGGGAAGCCT GGCTTACTAG GCCCCAAAGG TGAGCGGGGC 
AGCCCTGGGA CACCAGGACA GGTGGGACAG CCAGGCACCC CAGGATCTAG TGGTCCATAT 
GGCATCAAGG GCAAATCTGG GCTCCCAGGA GCACCAGGCT TCCCAGGCAT CTCAGGACAT 
CCTGGAAAGA AAGGAACAAG AGGCAAGAAA GGTCCTCCTG GATCAATTGT AAAGAAAGGG 
CTGCCAGGGC TAAAAGGCCT TCCTGGAAAT CCAGGCCTAG TAGGACTGAA AGGAAGCCCA 
GGCTCTCCAG GGGTCGCTGG GTTGCCAGCC CTCTCTGGAC CCAAGGGAGA GAAGGGGTCT 
GTTGGATTCG TAGGTTTTCC AGGAATACCA GGTCTGCCTG GTATTCCTGG AACAAGAGGA 
TTAAAAGGAA TTCCAGGATC AACTGGAAAA ATGGGACCAT CTGGACGTGC TGGTACTCCT 
GGTGAAAAGG GAGACAGAGG CAATCCGGGG CCAGTCGGAA TACCTAGTCC AAGACGTCCA 
ATGTCAAACC TTTGGCTCAA AGGAGACAAA GGCTCTCAAG GCTCAGCCGG ATCCAATGGA 
TTTCCTGGGC CAAGAGGTGA CAAAGGAGAG GCTGGTCGAC CTGGACCACC AGGCCTACCT 
GGAGCTCCTG GCCTCCCAGG CATTATCAAA GGAGTTAGTG GAAAGCCAGG GCCCCCTGGC 
TTCATGGGAA TCCGGGGCTT ACCTGGCCTG AAGGGGTCCT CTGGGATCAC AGGTTTCCCA 
GGAATGCCAG GAGAAAGTGG TTCACAAGGT ATCAGAGGGT CGCCTGGACT CCCAGGAGCA 
TCTGGTCTCC CAGGCCTGAA AGGAGACAAC GGCCAGACAG TTGAAATTTC CGGTAGCCCA 
GGACCCAAGG GACAGCCTGG CGAATCTGGT TTTAAAGGCA CAAAAGGAAG AGATGGACTA 
ATAGGCAATA TAGGCTTCCC TGGAAACAAA GGTGAAGATG GAAAAGTTGG TGTTTCTGGA 
GATGTTGGCC TTCCTGGAGC TCCAGGATTT CCAGGAGTTG CCGGCATGAG AGGAGAACCA 
GGACTTCCAG GTTCTTCTGG TCACCAAGGG GCAATTGGGC CTCTAGGATC CCCCGGATTA 
ATAGGACCCA AAGGCTTCCC TGGATTTCCT GGTTTACATG GACTGAATGG GCTTCCGGGC 
ACCAAGGGTA CCCATGGCAC TCCAGGACCT AGTATCACCG GTGTGCCTGG GCCTGCTGGT 
CTCCCTGGAC CCAAAGGAGA AAAAGGATAT CCAGGAATTG GCATCGGAGC TCCAGGGAAG 
CCGGGCCTGA GAGGGCAAAA AGGTGATCGA GGTTTCCCAG GTCTCCAGGG CCCTGCTGGT 
CTCCCCGGTG CCCCAGGCAT CTCCTTGCCC TCACTCATAG CAGGACAGCC TGGTGACCCC 
GGGCGACCAG GCCTAGATGG AGAACGAGGC CGCCCAGGCC CCGCTGGACC CCCAGGTCCC 
CCTGGGCCAT CCTCGAATCA AGGCGACACC GGAGACCCTG GCTTCCCTGG AATTCCTGGA 
CCTAAAGGGC CTAAGGGAGA CCAAGGAATT CCAGGTTTTT CTGGCCTCCC TGGAGAGCTA 
GGACTGAAAG GCATGAGAGG TGAGCCTGGC TTCATGGGGA CTCCAGGCAA GGTTGGGCCA 
CCTGGAGACC CAGGATTTCC CGGAATGAAG GGGAAGGCAG GGCCAAGAGG CTCTTCTGGC 
CTCCAAGGTG ATCCTGGACA AACACCAACT GCAGAAGCTG TCCAGGTTCC TCCTGGACCC 
TTGGGTCTAC CAGGGATCGA TGGCATCCCT GGCCTCACTG GGGACCCTGG GGCTCAAGGC 
CCTGTAGGCC TACAAGGCTC CAAAGGTTTA CCTGGCATCC CCGGTAAAGA TGGCCCCAGT 
GGGCTCCCAG GCCCACCTGG GGCTCTTGGT GATCCTGGTC TGCCTGGACT GCAAGGCCCT 
CCAGGATTTG AAGGAGCTCC AGGGCAGCAA GGCCCCTTCG GGATGCCTGG AATGCCTGGC 
CAGAGCATGA GAGTGGGCTA CACGTTGGTA AAGCACAGCC AGTCGGAACA GGTGCCCCCG 
TGTCCCATCG GGATGAGCCA GCTGTGGGTG GGGTACAGCT TACTGTTTGT GGAGGGGCAA 
GAGAAAGCCC ACAACCAGGA CCTGGGCTTT GCTGGCTCCT GTCTGCCCCG CTTCAGCACC 
ATGCCCTTCA TCTACTGCAA CATCAACGAG GTGTGCCACT ATGCCAGGCG CAATGATAAA 
TCTTACTGGC TCTCCACTAC CGCCCCTATC CCCATGATGC CCGTCAGCCA GACCCAGATT 
CCCCAGTACA TCAGCCGCTG CTCTGTGTGT GAGGCACCCT CGCAAGCCAT TGCTGTGCAC 
AGCCAGGACA TCACCATCCC GCAGTGCCCC CTGGGCTGGC GCAGCCTCTG GATTGGGTAC 
TCTTTCCTCA TGCACACTGC CGCTGGTGCC GAGGGTGGAG GCCAGTCCCT GGTCTCACCT 
GGCTCCTGCC TAGAGGACTT TCGGGCCACT CCTTTCATCG AATGCAGTGG TGCCCGAGGC 
ACCTGCCACT ACTTTGCAAA CAAGTACAGT TTCTGGTTGA CCACAGTGGA GGAGAGGCAG 
CAGTTTGGGG AGTTGCCTGT GTCTGAAACG CTGAAAGCTG GGCAGCTCCA CACTCGAGTC 
AGTCGCTGCC AGGTGTGTAT GAAAAGCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( NM_033641.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_378667.1 Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant B, mRNA.

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CloneID	OHu01057
Clone ID Related Accession (Same CDS sequence)	NM_033641.4 , NM_033641.3
Accession Version	NM_033641.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	collagen alpha-6(IV) chain isoform B precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL034369.1, CN313347.1, D21337.1, AL136080.6, U04845.1, BF726518.1, BQ331638.1 and CB050016.1. On Dec 19, 2013 this sequence version replaced NM_033641.2. Transcript Variant: This variant (B) utilizes the same 5' exon as variant 3 (exon 1B) but lacks several alternate in-frame exons. The resulting protein (isoform B) is shorter compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2146982 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACCGAGGA ACTGGCAGCA 
GCGGGAGAGA AGTCTTATGG AAAGCCATGT GGGGGCCAGG ACTGCAGTGG GAGCTGTCAG 
TGTTTTCCTG AGAAAGGAGC GAGAGGACGA CCTGGACCAA TTGGAATTCA AGGCCCAACA 
GGTCCTCAAG GATTCACTGG CTCTACTGGT TTATCGGGAT TGAAAGGAGA AAGGGGTTTC 
CCAGGCCTTC TGGGACCTTA TGGACCAAAA GGAGATAAGG GTCCCATGGG AGTTCCTGGC 
TTTCTTGGCA TCAATGGGAT TCCGGGCCAC CCTGGACAAC CAGGCCCCAG AGGCCCACCT 
GGTCTGGATG GCTGTAATGG AACTCAAGGA GCTGTTGGAT TTCCAGGCCC TGATGGCTAT 
CCTGGGCTTC TCGGACCACC CGGGCTTCCT GGTCAGAAAG GATCAAAAGG TGACCCTGTC 
CTTGCTCCAG GTAGTTTCAA AGGAATGAAG GGGGATCCTG GGCTGCCTGG ACTGGATGGA 
ATCACTGGCC CACAAGGAGC ACCCGGATTT CCTGGAGCTG TAGGACCTGC AGGACCACCA 
GGATTACAAG GTCCTCCAGG GCCTCCTGGT CCTCTTGGTC CTGATGGGAA TATGGGGCTA 
GGTTTTCAAG GAGAGAAAGG AGTCAAGGGG GATGTTGGCC TCCCTGGCCC AGCAGGACCT 
CCACCATCTA CTGGAGAGCT GGAATTCATG GGATTCCCCA AAGGGAAGAA AGGATCCAAG 
GGTGAACCAG GGCCTAAGGG TTTTCCAGGC ATAAGTGGCC CTCCAGGCTT CCCGGGCCTT 
GGAACTACTG GAGAAAAGGG AGAAAAGGGA GAAAAGGGAA TCCCTGGTTT GCCAGGACCT 
AGGGGTCCCA TGGGTTCAGA AGGAGTCCAA GGCCCTCCAG GGCAACAGGG CAAGAAAGGG 
ACCCTGGGAT TTCCTGGGCT TAATGGATTC CAAGGAATTG AGGGTCAAAA GGGTGACATT 
GGCCTGCCAG GCCCAGATGT TTTCATCGAT ATAGATGGTG CTGTGATCTC AGGTAATCCT 
GGAGATCCTG GTGTACCTGG CCTCCCAGGC CTTAAAGGAG ATGAAGGCAT CCAAGGCCTA 
CGTGGCCCTT CTGGTGTCCC TGGATTGCCA GCATTATCAG GTGTCCCAGG AGCCCTAGGG 
CCTCAGGGAT TTCCAGGGCT GAAGGGGGAC CAAGGAAACC CAGGCCGTAC CACAATTGGA 
GCAGCTGGCC TCCCTGGCAG AGATGGTTTG CCAGGCCCAC CAGGTCCACC AGGCCCACCT 
AGTCCAGAAT TTGAGACTGA AACTCTACAC AACAAAGAGT CAGGGTTCCC TGGTCTCCGA 
GGAGAACAAG GTCCAAAAGG AAACCTAGGC CTCAAAGGAA TAAAAGGAGA CTCAGGTTTC 
TGTGCTTGTG ACGGTGGTGT TCCCAACACT GGACCACCCG GGGAACCAGG CCCACCTGGT 
CCATGGGGTC TCATAGGCCT TCCAGGCCTT AAAGGAGCCA GAGGAGATCG AGGCTCTGGG 
GGTGCACAGG GCCCAGCAGG GGCTCCAGGC TTAGTTGGGC CTCTGGGTCC TTCAGGACCC 
AAAGGAAAGA AGGGGGAACC AATTCTCAGT ACAATCCAAG GAATGCCAGG AGATCGGGGT 
GATTCTGGCT CCCAGGGCTT CCGTGGTGTA ATAGGAGAAC CAGGCAAGGA CGGAGTACCA 
GGTTTACCAG GTCTGCCAGG CCTTCCGGGT GATGGTGGAC AGGGCTTCCC AGGTGAAAAG 
GGGTTACCTG GACTTCCTGG TGAAAAAGGC CATCCTGGTC CACCTGGCCT CCCAGGAAAT 
GGGTTACCAG GACTTCCTGG ACCCCGTGGG CTTCCTGGAG ATAAAGGCAA GGATGGATTA 
CCGGGACAAC AAGGCCTTCC CGGATCTAAG GGAATCACCC TGCCCTGTAT TATTCCTGGG 
TCATACGGTC CATCAGGATT TCCAGGCACT CCCGGATTCC CAGGCCCTAA AGGGTCTCGA 
GGCCTCCCTG GGACCCCAGG CCAGCCTGGG TCAAGTGGAA GTAAAGGAGA GCCAGGGAGT 
CCAGGATTGG TTCATCTTCC TGAATTACCA GGATTTCCTG GACCTCGTGG GGAGAAGGGC 
TTGCCTGGGT TTCCTGGGCT CCCTGGAAAA GATGGCTTGC CTGGGATGAT TGGCAGTCCA 
GGCTTACCTG GTTCCAAGGG AGCCACTGGT GACATCTTTG GTGCTGAAAA TGGTGCTCCG 
GGGGAACAAG GCCTACAAGG ATTAACAGGG CACAAAGGAT TTCTTGGAGA CTCTGGCCTT 
CCAGGACTCA AGGGTGTGCA CGGGAAGCCT GGCTTACTAG GCCCCAAAGG TGAGCGGGGC 
AGCCCTGGGA CACCAGGACA GGTGGGACAG CCAGGCACCC CAGGATCTAG TGGTCCATAT 
GGCATCAAGG GCAAATCTGG GCTCCCAGGA GCACCAGGCT TCCCAGGCAT CTCAGGACAT 
CCTGGAAAGA AAGGAACAAG AGGCAAGAAA GGTCCTCCTG GATCAATTGT AAAGAAAGGG 
CTGCCAGGGC TAAAAGGCCT TCCTGGAAAT CCAGGCCTAG TAGGACTGAA AGGAAGCCCA 
GGCTCTCCAG GGGTCGCTGG GTTGCCAGCC CTCTCTGGAC CCAAGGGAGA GAAGGGGTCT 
GTTGGATTCG TAGGTTTTCC AGGAATACCA GGTCTGCCTG GTATTCCTGG AACAAGAGGA 
TTAAAAGGAA TTCCAGGATC AACTGGAAAA ATGGGACCAT CTGGACGTGC TGGTACTCCT 
GGTGAAAAGG GAGACAGAGG CAATCCGGGG CCAGTCGGAA TACCTAGTCC AAGACGTCCA 
ATGTCAAACC TTTGGCTCAA AGGAGACAAA GGCTCTCAAG GCTCAGCCGG ATCCAATGGA 
TTTCCTGGGC CAAGAGGTGA CAAAGGAGAG GCTGGTCGAC CTGGACCACC AGGCCTACCT 
GGAGCTCCTG GCCTCCCAGG CATTATCAAA GGAGTTAGTG GAAAGCCAGG GCCCCCTGGC 
TTCATGGGAA TCCGGGGCTT ACCTGGCCTG AAGGGGTCCT CTGGGATCAC AGGTTTCCCA 
GGAATGCCAG GAGAAAGTGG TTCACAAGGT ATCAGAGGGT CGCCTGGACT CCCAGGAGCA 
TCTGGTCTCC CAGGCCTGAA AGGAGACAAC GGCCAGACAG TTGAAATTTC CGGTAGCCCA 
GGACCCAAGG GACAGCCTGG CGAATCTGGT TTTAAAGGCA CAAAAGGAAG AGATGGACTA 
ATAGGCAATA TAGGCTTCCC TGGAAACAAA GGTGAAGATG GAAAAGTTGG TGTTTCTGGA 
GATGTTGGCC TTCCTGGAGC TCCAGGATTT CCAGGAGTTG CCGGCATGAG AGGAGAACCA 
GGACTTCCAG GTTCTTCTGG TCACCAAGGG GCAATTGGGC CTCTAGGATC CCCCGGATTA 
ATAGGACCCA AAGGCTTCCC TGGATTTCCT GGTTTACATG GACTGAATGG GCTTCCGGGC 
ACCAAGGGTA CCCATGGCAC TCCAGGACCT AGTATCACCG GTGTGCCTGG GCCTGCTGGT 
CTCCCTGGAC CCAAAGGAGA AAAAGGATAT CCAGGAATTG GCATCGGAGC TCCAGGGAAG 
CCGGGCCTGA GAGGGCAAAA AGGTGATCGA GGTTTCCCAG GTCTCCAGGG CCCTGCTGGT 
CTCCCCGGTG CCCCAGGCAT CTCCTTGCCC TCACTCATAG CAGGACAGCC TGGTGACCCC 
GGGCGACCAG GCCTAGATGG AGAACGAGGC CGCCCAGGCC CCGCTGGACC CCCAGGTCCC 
CCTGGGCCAT CCTCGAATCA AGGCGACACC GGAGACCCTG GCTTCCCTGG AATTCCTGGA 
CCTAAAGGGC CTAAGGGAGA CCAAGGAATT CCAGGTTTTT CTGGCCTCCC TGGAGAGCTA 
GGACTGAAAG GCATGAGAGG TGAGCCTGGC TTCATGGGGA CTCCAGGCAA GGTTGGGCCA 
CCTGGAGACC CAGGATTTCC CGGAATGAAG GGGAAGGCAG GGCCAAGAGG CTCTTCTGGC 
CTCCAAGGTG ATCCTGGACA AACACCAACT GCAGAAGCTG TCCAGGTTCC TCCTGGACCC 
TTGGGTCTAC CAGGGATCGA TGGCATCCCT GGCCTCACTG GGGACCCTGG GGCTCAAGGC 
CCTGTAGGCC TACAAGGCTC CAAAGGTTTA CCTGGCATCC CCGGTAAAGA TGGCCCCAGT 
GGGCTCCCAG GCCCACCTGG GGCTCTTGGT GATCCTGGTC TGCCTGGACT GCAAGGCCCT 
CCAGGATTTG AAGGAGCTCC AGGGCAGCAA GGCCCCTTCG GGATGCCTGG AATGCCTGGC 
CAGAGCATGA GAGTGGGCTA CACGTTGGTA AAGCACAGCC AGTCGGAACA GGTGCCCCCG 
TGTCCCATCG GGATGAGCCA GCTGTGGGTG GGGTACAGCT TACTGTTTGT GGAGGGGCAA 
GAGAAAGCCC ACAACCAGGA CCTGGGCTTT GCTGGCTCCT GTCTGCCCCG CTTCAGCACC 
ATGCCCTTCA TCTACTGCAA CATCAACGAG GTGTGCCACT ATGCCAGGCG CAATGATAAA 
TCTTACTGGC TCTCCACTAC CGCCCCTATC CCCATGATGC CCGTCAGCCA GACCCAGATT 
CCCCAGTACA TCAGCCGCTG CTCTGTGTGT GAGGCACCCT CGCAAGCCAT TGCTGTGCAC 
AGCCAGGACA TCACCATCCC GCAGTGCCCC CTGGGCTGGC GCAGCCTCTG GATTGGGTAC 
TCTTTCCTCA TGCACACTGC CGCTGGTGCC GAGGGTGGAG GCCAGTCCCT GGTCTCACCT 
GGCTCCTGCC TAGAGGACTT TCGGGCCACT CCTTTCATCG AATGCAGTGG TGCCCGAGGC 
ACCTGCCACT ACTTTGCAAA CAAGTACAGT TTCTGGTTGA CCACAGTGGA GGAGAGGCAG 
CAGTTTGGGG AGTTGCCTGT GTCTGAAACG CTGAAAGCTG GGCAGCTCCA CACTCGAGTC 
AGTCGCTGCC AGGTGTGTAT GAAAAGCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_378667.1
CDS	235..5307
Translation	MHPGLWLLLVTLCLTEELAAAGEKSYGKPCGGQDCSGSCQCFPEKGARGRPGPIGIQGPTGPQGFTGSTGLSGLKGERGFPGLLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYPGLLGPPGLPGQKGSKGDPVLAPGSFKGMKGDPGLPGLDGITGPQGAPGFPGAVGPAGPPGLQGPPGPPGPLGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPKGFPGISGPPGFPGLGTTGEKGEKGEKGIPGLPGPRGPMGSEGVQGPPGQQGKKGTLGFPGLNGFQGIEGQKGDIGLPGPDVFIDIDGAVISGNPGDPGVPGLPGLKGDEGIQGLRGPSGVPGLPALSGVPGALGPQGFPGLKGDQGNPGRTTIGAAGLPGRDGLPGPPGPPGPPSPEFETETLHNKESGFPGLRGEQGPKGNLGLKGIKGDSGFCACDGGVPNTGPPGEPGPPGPWGLIGLPGLKGARGDRGSGGAQGPAGAPGLVGPLGPSGPKGKKGEPILSTIQGMPGDRGDSGSQGFRGVIGEPGKDGVPGLPGLPGLPGDGGQGFPGEKGLPGLPGEKGHPGPPGLPGNGLPGLPGPRGLPGDKGKDGLPGQQGLPGSKGITLPCIIPGSYGPSGFPGTPGFPGPKGSRGLPGTPGQPGSSGSKGEPGSPGLVHLPELPGFPGPRGEKGLPGFPGLPGKDGLPGMIGSPGLPGSKGATGDIFGAENGAPGEQGLQGLTGHKGFLGDSGLPGLKGVHGKPGLLGPKGERGSPGTPGQVGQPGTPGSSGPYGIKGKSGLPGAPGFPGISGHPGKKGTRGKKGPPGSIVKKGLPGLKGLPGNPGLVGLKGSPGSPGVAGLPALSGPKGEKGSVGFVGFPGIPGLPGIPGTRGLKGIPGSTGKMGPSGRAGTPGEKGDRGNPGPVGIPSPRRPMSNLWLKGDKGSQGSAGSNGFPGPRGDKGEAGRPGPPGLPGAPGLPGIIKGVSGKPGPPGFMGIRGLPGLKGSSGITGFPGMPGESGSQGIRGSPGLPGASGLPGLKGDNGQTVEISGSPGPKGQPGESGFKGTKGRDGLIGNIGFPGNKGEDGKVGVSGDVGLPGAPGFPGVAGMRGEPGLPGSSGHQGAIGPLGSPGLIGPKGFPGFPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGYPGIGIGAPGKPGLRGQKGDRGFPGLQGPAGLPGAPGISLPSLIAGQPGDPGRPGLDGERGRPGPAGPPGPPGPSSNQGDTGDPGFPGIPGPKGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGFPGMKGKAGPRGSSGLQGDPGQTPTAEAVQVPPGPLGLPGIDGIPGLTGDPGAQGPVGLQGSKGLPGIPGKDGPSGLPGPPGALGDPGLPGLQGPPGFEGAPGQQGPFGMPGMPGQSMRVGYTLVKHSQSEQVPPCPIGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVSQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGELPVSETLKAGQLHTRVSRCQVCMKSL

Target ORF information:

RefSeq Version	NM_033641.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033641.3

ORF Insert Sequence:

ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACCGAGGA ACTGGCAGCA 
GCGGGAGAGA AGTCTTATGG AAAGCCATGT GGGGGCCAGG ACTGCAGTGG GAGCTGTCAG 
TGTTTTCCTG AGAAAGGAGC GAGAGGACGA CCTGGACCAA TTGGAATTCA AGGCCCAACA 
GGTCCTCAAG GATTCACTGG CTCTACTGGT TTATCGGGAT TGAAAGGAGA AAGGGGTTTC 
CCAGGCCTTC TGGGACCTTA TGGACCAAAA GGAGATAAGG GTCCCATGGG AGTTCCTGGC 
TTTCTTGGCA TCAATGGGAT TCCGGGCCAC CCTGGACAAC CAGGCCCCAG AGGCCCACCT 
GGTCTGGATG GCTGTAATGG AACTCAAGGA GCTGTTGGAT TTCCAGGCCC TGATGGCTAT 
CCTGGGCTTC TCGGACCACC CGGGCTTCCT GGTCAGAAAG GATCAAAAGG TGACCCTGTC 
CTTGCTCCAG GTAGTTTCAA AGGAATGAAG GGGGATCCTG GGCTGCCTGG ACTGGATGGA 
ATCACTGGCC CACAAGGAGC ACCCGGATTT CCTGGAGCTG TAGGACCTGC AGGACCACCA 
GGATTACAAG GTCCTCCAGG GCCTCCTGGT CCTCTTGGTC CTGATGGGAA TATGGGGCTA 
GGTTTTCAAG GAGAGAAAGG AGTCAAGGGG GATGTTGGCC TCCCTGGCCC AGCAGGACCT 
CCACCATCTA CTGGAGAGCT GGAATTCATG GGATTCCCCA AAGGGAAGAA AGGATCCAAG 
GGTGAACCAG GGCCTAAGGG TTTTCCAGGC ATAAGTGGCC CTCCAGGCTT CCCGGGCCTT 
GGAACTACTG GAGAAAAGGG AGAAAAGGGA GAAAAGGGAA TCCCTGGTTT GCCAGGACCT 
AGGGGTCCCA TGGGTTCAGA AGGAGTCCAA GGCCCTCCAG GGCAACAGGG CAAGAAAGGG 
ACCCTGGGAT TTCCTGGGCT TAATGGATTC CAAGGAATTG AGGGTCAAAA GGGTGACATT 
GGCCTGCCAG GCCCAGATGT TTTCATCGAT ATAGATGGTG CTGTGATCTC AGGTAATCCT 
GGAGATCCTG GTGTACCTGG CCTCCCAGGC CTTAAAGGAG ATGAAGGCAT CCAAGGCCTA 
CGTGGCCCTT CTGGTGTCCC TGGATTGCCA GCATTATCAG GTGTCCCAGG AGCCCTAGGG 
CCTCAGGGAT TTCCAGGGCT GAAGGGGGAC CAAGGAAACC CAGGCCGTAC CACAATTGGA 
GCAGCTGGCC TCCCTGGCAG AGATGGTTTG CCAGGCCCAC CAGGTCCACC AGGCCCACCT 
AGTCCAGAAT TTGAGACTGA AACTCTACAC AACAAAGAGT CAGGGTTCCC TGGTCTCCGA 
GGAGAACAAG GTCCAAAAGG AAACCTAGGC CTCAAAGGAA TAAAAGGAGA CTCAGGTTTC 
TGTGCTTGTG ACGGTGGTGT TCCCAACACT GGACCACCCG GGGAACCAGG CCCACCTGGT 
CCATGGGGTC TCATAGGCCT TCCAGGCCTT AAAGGAGCCA GAGGAGATCG AGGCTCTGGG 
GGTGCACAGG GCCCAGCAGG GGCTCCAGGC TTAGTTGGGC CTCTGGGTCC TTCAGGACCC 
AAAGGAAAGA AGGGGGAACC AATTCTCAGT ACAATCCAAG GAATGCCAGG AGATCGGGGT 
GATTCTGGCT CCCAGGGCTT CCGTGGTGTA ATAGGAGAAC CAGGCAAGGA CGGAGTACCA 
GGTTTACCAG GTCTGCCAGG CCTTCCGGGT GATGGTGGAC AGGGCTTCCC AGGTGAAAAG 
GGGTTACCTG GACTTCCTGG TGAAAAAGGC CATCCTGGTC CACCTGGCCT CCCAGGAAAT 
GGGTTACCAG GACTTCCTGG ACCCCGTGGG CTTCCTGGAG ATAAAGGCAA GGATGGATTA 
CCGGGACAAC AAGGCCTTCC CGGATCTAAG GGAATCACCC TGCCCTGTAT TATTCCTGGG 
TCATACGGTC CATCAGGATT TCCAGGCACT CCCGGATTCC CAGGCCCTAA AGGGTCTCGA 
GGCCTCCCTG GGACCCCAGG CCAGCCTGGG TCAAGTGGAA GTAAAGGAGA GCCAGGGAGT 
CCAGGATTGG TTCATCTTCC TGAATTACCA GGATTTCCTG GACCTCGTGG GGAGAAGGGC 
TTGCCTGGGT TTCCTGGGCT CCCTGGAAAA GATGGCTTGC CTGGGATGAT TGGCAGTCCA 
GGCTTACCTG GTTCCAAGGG AGCCACTGGT GACATCTTTG GTGCTGAAAA TGGTGCTCCG 
GGGGAACAAG GCCTACAAGG ATTAACAGGG CACAAAGGAT TTCTTGGAGA CTCTGGCCTT 
CCAGGACTCA AGGGTGTGCA CGGGAAGCCT GGCTTACTAG GCCCCAAAGG TGAGCGGGGC 
AGCCCTGGGA CACCAGGACA GGTGGGACAG CCAGGCACCC CAGGATCTAG TGGTCCATAT 
GGCATCAAGG GCAAATCTGG GCTCCCAGGA GCACCAGGCT TCCCAGGCAT CTCAGGACAT 
CCTGGAAAGA AAGGAACAAG AGGCAAGAAA GGTCCTCCTG GATCAATTGT AAAGAAAGGG 
CTGCCAGGGC TAAAAGGCCT TCCTGGAAAT CCAGGCCTAG TAGGACTGAA AGGAAGCCCA 
GGCTCTCCAG GGGTCGCTGG GTTGCCAGCC CTCTCTGGAC CCAAGGGAGA GAAGGGGTCT 
GTTGGATTCG TAGGTTTTCC AGGAATACCA GGTCTGCCTG GTATTCCTGG AACAAGAGGA 
TTAAAAGGAA TTCCAGGATC AACTGGAAAA ATGGGACCAT CTGGACGTGC TGGTACTCCT 
GGTGAAAAGG GAGACAGAGG CAATCCGGGG CCAGTCGGAA TACCTAGTCC AAGACGTCCA 
ATGTCAAACC TTTGGCTCAA AGGAGACAAA GGCTCTCAAG GCTCAGCCGG ATCCAATGGA 
TTTCCTGGGC CAAGAGGTGA CAAAGGAGAG GCTGGTCGAC CTGGACCACC AGGCCTACCT 
GGAGCTCCTG GCCTCCCAGG CATTATCAAA GGAGTTAGTG GAAAGCCAGG GCCCCCTGGC 
TTCATGGGAA TCCGGGGCTT ACCTGGCCTG AAGGGGTCCT CTGGGATCAC AGGTTTCCCA 
GGAATGCCAG GAGAAAGTGG TTCACAAGGT ATCAGAGGGT CGCCTGGACT CCCAGGAGCA 
TCTGGTCTCC CAGGCCTGAA AGGAGACAAC GGCCAGACAG TTGAAATTTC CGGTAGCCCA 
GGACCCAAGG GACAGCCTGG CGAATCTGGT TTTAAAGGCA CAAAAGGAAG AGATGGACTA 
ATAGGCAATA TAGGCTTCCC TGGAAACAAA GGTGAAGATG GAAAAGTTGG TGTTTCTGGA 
GATGTTGGCC TTCCTGGAGC TCCAGGATTT CCAGGAGTTG CCGGCATGAG AGGAGAACCA 
GGACTTCCAG GTTCTTCTGG TCACCAAGGG GCAATTGGGC CTCTAGGATC CCCCGGATTA 
ATAGGACCCA AAGGCTTCCC TGGATTTCCT GGTTTACATG GACTGAATGG GCTTCCGGGC 
ACCAAGGGTA CCCATGGCAC TCCAGGACCT AGTATCACCG GTGTGCCTGG GCCTGCTGGT 
CTCCCTGGAC CCAAAGGAGA AAAAGGATAT CCAGGAATTG GCATCGGAGC TCCAGGGAAG 
CCGGGCCTGA GAGGGCAAAA AGGTGATCGA GGTTTCCCAG GTCTCCAGGG CCCTGCTGGT 
CTCCCCGGTG CCCCAGGCAT CTCCTTGCCC TCACTCATAG CAGGACAGCC TGGTGACCCC 
GGGCGACCAG GCCTAGATGG AGAACGAGGC CGCCCAGGCC CCGCTGGACC CCCAGGTCCC 
CCTGGGCCAT CCTCGAATCA AGGCGACACC GGAGACCCTG GCTTCCCTGG AATTCCTGGA 
CCTAAAGGGC CTAAGGGAGA CCAAGGAATT CCAGGTTTTT CTGGCCTCCC TGGAGAGCTA 
GGACTGAAAG GCATGAGAGG TGAGCCTGGC TTCATGGGGA CTCCAGGCAA GGTTGGGCCA 
CCTGGAGACC CAGGATTTCC CGGAATGAAG GGGAAGGCAG GGCCAAGAGG CTCTTCTGGC 
CTCCAAGGTG ATCCTGGACA AACACCAACT GCAGAAGCTG TCCAGGTTCC TCCTGGACCC 
TTGGGTCTAC CAGGGATCGA TGGCATCCCT GGCCTCACTG GGGACCCTGG GGCTCAAGGC 
CCTGTAGGCC TACAAGGCTC CAAAGGTTTA CCTGGCATCC CCGGTAAAGA TGGCCCCAGT 
GGGCTCCCAG GCCCACCTGG GGCTCTTGGT GATCCTGGTC TGCCTGGACT GCAAGGCCCT 
CCAGGATTTG AAGGAGCTCC AGGGCAGCAA GGCCCCTTCG GGATGCCTGG AATGCCTGGC 
CAGAGCATGA GAGTGGGCTA CACGTTGGTA AAGCACAGCC AGTCGGAACA GGTGCCCCCG 
TGTCCCATCG GGATGAGCCA GCTGTGGGTG GGGTACAGCT TACTGTTTGT GGAGGGGCAA 
GAGAAAGCCC ACAACCAGGA CCTGGGCTTT GCTGGCTCCT GTCTGCCCCG CTTCAGCACC 
ATGCCCTTCA TCTACTGCAA CATCAACGAG GTGTGCCACT ATGCCAGGCG CAATGATAAA 
TCTTACTGGC TCTCCACTAC CGCCCCTATC CCCATGATGC CCGTCAGCCA GACCCAGATT 
CCCCAGTACA TCAGCCGCTG CTCTGTGTGT GAGGCACCCT CGCAAGCCAT TGCTGTGCAC 
AGCCAGGACA TCACCATCCC GCAGTGCCCC CTGGGCTGGC GCAGCCTCTG GATTGGGTAC 
TCTTTCCTCA TGCACACTGC CGCTGGTGCC GAGGGTGGAG GCCAGTCCCT GGTCTCACCT 
GGCTCCTGCC TAGAGGACTT TCGGGCCACT CCTTTCATCG AATGCAGTGG TGCCCGAGGC 
ACCTGCCACT ACTTTGCAAA CAAGTACAGT TTCTGGTTGA CCACAGTGGA GGAGAGGCAG 
CAGTTTGGGG AGTTGCCTGT GTCTGAAACG CTGAAAGCTG GGCAGCTCCA CACTCGAGTC 
AGTCGCTGCC AGGTGTGTAT GAAAAGCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( NM_001287759.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274688.1 Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant 4, mRNA.

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CloneID	OHu01135
Clone ID Related Accession (Same CDS sequence)	NM_001287759.1
Accession Version	NM_001287759.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Homo sapiens(human)
Product	collagen alpha-6(IV) chain isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL034369.1, CN313347.1, BC000874.1, BC144669.1, CN313332.1, AL136080.6, EU182215.1, DB260304.1 and CB050016.1. On Dec 19, 2013 this sequence version replaced XM_005262074.1. Transcript Variant: This variant (4) lacks several alternate in-frame exons compared to variant 3. The resulting protein (isoform 4) is shorter compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144669.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACCGAGGA ACTGGCAGCA 
GCGGGAGAGA AGTCTTATGG AAAGCCATGT GGGGGCCAGG ACTGCAGTGG GAGCTGTCAG 
TGTTTTCCTG AGAAAGGAGC GAGAGGACGA CCTGGACCAA TTGGAATTCA AGGCCCAACA 
GGTCCTCAAG GATTCACTGG CTCTACTGGT TTATCGGGAT TGAAAGGAGA AAGGGGTTTC 
CCAGGCCTTC TGGGACCTTA TGGACCAAAA GGAGATAAGG GTCCCATGGG AGTTCCTGGC 
TTTCTTGGCA TCAATGGGAT TCCGGGCCAC CCTGGACAAC CAGGCCCCAG AGGCCCACCT 
GGTCTGGATG GCTGTAATGG AACTCAAGGA GCTGTTGGAT TTCCAGGCCC TGATGGCTAT 
CCTGGGCTTC TCGGACCACC CGGGCTTCCT GGTCAGAAAG GATCAAAAGG TGACCCTGTC 
CTTGCTCCAG GTAGTTTCAA AGGAATGAAG GGGGATCCTG GGCTGCCTGG ACTGGATGGA 
ATCACTGGCC CACAAGGAGC ACCCGGATTT CCTGGAGCTG TAGGACCTGC AGGACCACCA 
GGATTACAAG GTCCTCCAGG GCCTCCTGGT CCTCTTGGTC CTGATGGGAA TATGGGGCTA 
GGTTTTCAAG GAGAGAAAGG AGTCAAGGGG GATGTTGGCC TCCCTGGCCC AGCAGGACCT 
CCACCATCTA CTGGAGAGCT GGAATTCATG GGATTCCCCA AAGGGAAGAA AGGATCCAAG 
GGTGAACCAG GGCCTAAGGG TTTTCCAGGC ATAAGTGGCC CTCCAGGCTT CCCGGGCCTT 
GGAACTACTG GAGAAAAGGG AGAAAAGGGA GAAAAGGGAA TCCCTGGTTT GCCAGGACCT 
AGGGGTCCCA TGGGTTCAGA AGGAGTCCAA GGCCCTCCAG GGCAACAGGG CAAGAAAGGG 
ACCCTGGGAT TTCCTGGGCT TAATGGATTC CAAGGAATTG AGGGTCAAAA GGGTGACATT 
GGCCTGCCAG GCCCAGATGT TTTCATCGAT ATAGATGGTG CTGTGATCTC AGGTAATCCT 
GGAGATCCTG GTGTACCTGG CCTCCCAGGC CTTAAAGGAG ATGAAGGCAT CCAAGGCCTA 
CGTGGCCCTT CTGGTGTCCC TGGATTGCCA GCATTATCAG GTGTCCCAGG AGCCCTAGGG 
CCTCAGGGAT TTCCAGGGCT GAAGGGGGAC CAAGGAAACC CAGGCCGTAC CACAATTGGA 
GCAGCTGGCC TCCCTGGCAG AGATGGTTTG CCAGGCCCAC CAGGTCCACC AGGCCCACCT 
AGTCCAGAAT TTGAGACTGA AACTCTACAC AACAAAGAGT CAGGGTTCCC TGGTCTCCGA 
GGAGAACAAG GTCCAAAAGG AAACCTAGGC CTCAAAGGAA TAAAAGGAGA CTCAGGTTTC 
TGTGCTTGTG ACGGTGGTGT TCCCAACACT GGACCACCCG GGGAACCAGG CCCACCTGGT 
CCATGGGGTC TCATAGGCCT TCCAGGCCTT AAAGGAGCCA GAGGAGATCG AGGCTCTGGG 
GGTGCACAGG GCCCAGCAGG GGCTCCAGGC TTAGTTGGGC CTCTGGGTCC TTCAGGACCC 
AAAGGAAAGA AGGGGGAACC AATTCTCAGT ACAATCCAAG GAATGCCAGG AGATCGGGGT 
GATTCTGGCT CCCAGGGCTT CCGTGGTGTA ATAGGAGAAC CAGGCAAGGA CGGAGTACCA 
GGTTTACCAG GTCTGCCAGG CCTTCCGGGT GATGGTGGAC AGGGCTTCCC AGGTGAAAAG 
GGGTTACCTG GACTTCCTGG TGAAAAAGGC CATCCTGGTC CACCTGGCCT CCCAGGAAAT 
GGGTTACCAG GACTTCCTGG ACCCCGTGGG CTTCCTGGAG ATAAAGGCAA GGATGGATTA 
CCGGGACAAC AAGGCCTTCC CGGATCTAAG GGAATCACCC TGCCCTGTAT TATTCCTGGG 
TCATACGGTC CATCAGGATT TCCAGGCACT CCCGGATTCC CAGGCCCTAA AGGGTCTCGA 
GGCCTCCCTG GGACCCCAGG CCAGCCTGGG TCAAGTGGAA GTAAAGGAGA GCCAGGGAGT 
CCAGGATTGG TTCATCTTCC TGAATTACCA GGATTTCCTG GACCTCGTGG GGAGAAGGGC 
TTGCCTGGGT TTCCTGGGCT CCCTGGAAAA GATGGCTTGC CTGGGATGAT TGGCAGTCCA 
GGCTTACCTG GTTCCAAGGG AGCCACTGGT GACATCTTTG GTGCTGAAAA TGGTGCTCCG 
GGGGAACAAG GCCTACAAGG ATTAACAGGG CACAAAGGAT TTCTTGGAGA CTCTGGCCTT 
CCAGGACTCA AGGGTGTGCA CGGGAAGCCT GGCTTACTAG GCCCCAAAGG TGAGCGGGGC 
AGCCCTGGGA CACCAGGACA GGTGGGACAG CCAGGCACCC CAGGATCTAG TGGTCCATAT 
GGCATCAAGG GCAAATCTGG GCTCCCAGGA GCACCAGGCT TCCCAGGCAT CTCAGGACAT 
CCTGGAAAGA AAGGAACAAG AGGCAAGAAA GGTCCTCCTG GATCAATTGT AAAGAAAGGG 
CTGCCAGGGC TAAAAGGCCT TCCTGGAAAT CCAGGCCTAG TAGGACTGAA AGGAAGCCCA 
GGCTCTCCAG GGGTCGCTGG GTTGCCAGCC CTCTCTGGAC CCAAGGGAGA GAAGGGGTCT 
GTTGGATTCG TAGGTTTTCC AGGAATACCA GGTCTGCCTG GTATTCCTGG AACAAGAGGA 
TTAAAAGGAA TTCCAGGATC AACTGGAAAA ATGGGACCAT CTGGACGTGC TGGTACTCCT 
GGTGAAAAGG GAGACAGAGG CAATCCGGGG CCAGTCGGAA TACCTAGTCC AAGACGTCCA 
ATGTCAAACC TTTGGCTCAA AGGAGACAAA GGCTCTCAAG GCTCAGCCGG ATCCAATGGA 
TTTCCTGGGC CAAGAGGTGA CAAAGGAGAG GCTGGTCGAC CTGGACCACC AGGCCTACCT 
GGAGCTCCTG GCCTCCCAGG CATTATCAAA GGAGTTAGTG GAAAGCCAGG GCCCCCTGGC 
TTCATGGGAA TCCGGGGCTT ACCTGGCCTG AAGGGGTCCT CTGGGATCAC AGGTTTCCCA 
GGAATGCCAG GAGAAAGTGG TTCACAAGGT ATCAGAGGGT CGCCTGGACT CCCAGGAGCA 
TCTGGTCTCC CAGGCCTGAA AGGAGACAAC GGCCAGACAG TTGAAATTTC CGGTAGCCCA 
GGACCCAAGG GACAGCCTGG CGAATCTGGT TTTAAAGGCA CAAAAGGAAG AGATGGACTA 
ATAGGCAATA TAGGCTTCCC TGGAAACAAA GGTGAAGATG GAAAAGTTGG TGTTTCTGGA 
GATGTTGGCC TTCCTGGAGC TCCAGGATTT CCAGGAGTTG CCGGCATGAG AGGAGAACCA 
GGACTTCCAG GTTCTTCTGG TCACCAAGGG GCAATTGGGC CTCTAGGATC CCCCGGATTA 
ATAGGACCCA AAGGACCTAG TATCACCGGT GTGCCTGGGC CTGCTGGTCT CCCTGGACCC 
AAAGGAGAAA AAGGATATCC AGGAATTGGC ATCGGAGCTC CAGGGAAGCC GGGCCTGAGA 
GGGCAAAAAG GTGATCGAGG TTTCCCAGGT CTCCAGGGCC CTGCTGGTCT CCCCGGTGCC 
CCAGGCATCT CCTTGCCCTC ACTCATAGCA GGACAGCCTG GTGACCCCGG GCGACCAGGC 
CTAGATGGAG AACGAGGCCG CCCAGGCCCC GCTGGACCCC CAGGTCCCCC TGGGCCATCC 
TCGAATCAAG GCGACACCGG AGACCCTGGC TTCCCTGGAA TTCCTGGACC TAAAGGGCCT 
AAGGGAGACC AAGGAATTCC AGGTTTTTCT GGCCTCCCTG GAGAGCTAGG ACTGAAAGGC 
ATGAGAGGTG AGCCTGGCTT CATGGGGACT CCAGGCAAGG TTGGGCCACC TGGAGACCCA 
GGATTTCCCG GAATGAAGGG GAAGGCAGGG CCAAGAGGCT CTTCTGGCCT CCAAGGTGAT 
CCTGGACAAA CACCAACTGC AGAAGCTGTC CAGGTTCCTC CTGGACCCTT GGGTCTACCA 
GGGATCGATG GCATCCCTGG CCTCACTGGG GACCCTGGGG CTCAAGGCCC TGTAGGCCTA 
CAAGGCTCCA AAGGTTTACC TGGCATCCCC GGTAAAGATG GCCCCAGTGG GCTCCCAGGC 
CCACCTGGGG CTCTTGGTGA TCCTGGTCTG CCTGGACTGC AAGGCCCTCC AGGATTTGAA 
GGAGCTCCAG GGCAGCAAGG CCCCTTCGGG ATGCCTGGAA TGCCTGGCCA GAGCATGAGA 
GTGGGCTACA CGTTGGTAAA GCACAGCCAG TCGGAACAGG TGCCCCCGTG TCCCATCGGG 
ATGAGCCAGC TGTGGGTGGG GTACAGCTTA CTGTTTGTGG AGGGGCAAGA GAAAGCCCAC 
AACCAGGACC TGGGCTTTGC TGGCTCCTGT CTGCCCCGCT TCAGCACCAT GCCCTTCATC 
TACTGCAACA TCAACGAGGT GTGCCACTAT GCCAGGCGCA ATGATAAATC TTACTGGCTC 
TCCACTACCG CCCCTATCCC CATGATGCCC GTCAGCCAGA CCCAGATTCC CCAGTACATC 
AGCCGCTGCT CTGTGTGTGA GGCACCCTCG CAAGCCATTG CTGTGCACAG CCAGGACATC 
ACCATCCCGC AGTGCCCCCT GGGCTGGCGC AGCCTCTGGA TTGGGTACTC TTTCCTCATG 
CACACTGCCG CTGGTGCCGA GGGTGGAGGC CAGTCCCTGG TCTCACCTGG CTCCTGCCTA 
GAGGACTTTC GGGCCACTCC TTTCATCGAA TGCAGTGGTG CCCGAGGCAC CTGCCACTAC 
TTTGCAAACA AGTACAGTTT CTGGTTGACC ACAGTGGAGG AGAGGCAGCA GTTTGGGGAG 
TTGCCTGTGT CTGAAACGCT GAAAGCTGGG CAGCTCCACA CTCGAGTCAG TCGCTGCCAG 
GTGTGTATGA AAAGCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274688.1
CDS	235..5235
Translation	MHPGLWLLLVTLCLTEELAAAGEKSYGKPCGGQDCSGSCQCFPEKGARGRPGPIGIQGPTGPQGFTGSTGLSGLKGERGFPGLLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYPGLLGPPGLPGQKGSKGDPVLAPGSFKGMKGDPGLPGLDGITGPQGAPGFPGAVGPAGPPGLQGPPGPPGPLGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPKGFPGISGPPGFPGLGTTGEKGEKGEKGIPGLPGPRGPMGSEGVQGPPGQQGKKGTLGFPGLNGFQGIEGQKGDIGLPGPDVFIDIDGAVISGNPGDPGVPGLPGLKGDEGIQGLRGPSGVPGLPALSGVPGALGPQGFPGLKGDQGNPGRTTIGAAGLPGRDGLPGPPGPPGPPSPEFETETLHNKESGFPGLRGEQGPKGNLGLKGIKGDSGFCACDGGVPNTGPPGEPGPPGPWGLIGLPGLKGARGDRGSGGAQGPAGAPGLVGPLGPSGPKGKKGEPILSTIQGMPGDRGDSGSQGFRGVIGEPGKDGVPGLPGLPGLPGDGGQGFPGEKGLPGLPGEKGHPGPPGLPGNGLPGLPGPRGLPGDKGKDGLPGQQGLPGSKGITLPCIIPGSYGPSGFPGTPGFPGPKGSRGLPGTPGQPGSSGSKGEPGSPGLVHLPELPGFPGPRGEKGLPGFPGLPGKDGLPGMIGSPGLPGSKGATGDIFGAENGAPGEQGLQGLTGHKGFLGDSGLPGLKGVHGKPGLLGPKGERGSPGTPGQVGQPGTPGSSGPYGIKGKSGLPGAPGFPGISGHPGKKGTRGKKGPPGSIVKKGLPGLKGLPGNPGLVGLKGSPGSPGVAGLPALSGPKGEKGSVGFVGFPGIPGLPGIPGTRGLKGIPGSTGKMGPSGRAGTPGEKGDRGNPGPVGIPSPRRPMSNLWLKGDKGSQGSAGSNGFPGPRGDKGEAGRPGPPGLPGAPGLPGIIKGVSGKPGPPGFMGIRGLPGLKGSSGITGFPGMPGESGSQGIRGSPGLPGASGLPGLKGDNGQTVEISGSPGPKGQPGESGFKGTKGRDGLIGNIGFPGNKGEDGKVGVSGDVGLPGAPGFPGVAGMRGEPGLPGSSGHQGAIGPLGSPGLIGPKGPSITGVPGPAGLPGPKGEKGYPGIGIGAPGKPGLRGQKGDRGFPGLQGPAGLPGAPGISLPSLIAGQPGDPGRPGLDGERGRPGPAGPPGPPGPSSNQGDTGDPGFPGIPGPKGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGFPGMKGKAGPRGSSGLQGDPGQTPTAEAVQVPPGPLGLPGIDGIPGLTGDPGAQGPVGLQGSKGLPGIPGKDGPSGLPGPPGALGDPGLPGLQGPPGFEGAPGQQGPFGMPGMPGQSMRVGYTLVKHSQSEQVPPCPIGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVSQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGELPVSETLKAGQLHTRVSRCQVCMKSL

Target ORF information:

RefSeq Version	NM_001287759.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287759.1

ORF Insert Sequence:

ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACCGAGGA ACTGGCAGCA 
GCGGGAGAGA AGTCTTATGG AAAGCCATGT GGGGGCCAGG ACTGCAGTGG GAGCTGTCAG 
TGTTTTCCTG AGAAAGGAGC GAGAGGACGA CCTGGACCAA TTGGAATTCA AGGCCCAACA 
GGTCCTCAAG GATTCACTGG CTCTACTGGT TTATCGGGAT TGAAAGGAGA AAGGGGTTTC 
CCAGGCCTTC TGGGACCTTA TGGACCAAAA GGAGATAAGG GTCCCATGGG AGTTCCTGGC 
TTTCTTGGCA TCAATGGGAT TCCGGGCCAC CCTGGACAAC CAGGCCCCAG AGGCCCACCT 
GGTCTGGATG GCTGTAATGG AACTCAAGGA GCTGTTGGAT TTCCAGGCCC TGATGGCTAT 
CCTGGGCTTC TCGGACCACC CGGGCTTCCT GGTCAGAAAG GATCAAAAGG TGACCCTGTC 
CTTGCTCCAG GTAGTTTCAA AGGAATGAAG GGGGATCCTG GGCTGCCTGG ACTGGATGGA 
ATCACTGGCC CACAAGGAGC ACCCGGATTT CCTGGAGCTG TAGGACCTGC AGGACCACCA 
GGATTACAAG GTCCTCCAGG GCCTCCTGGT CCTCTTGGTC CTGATGGGAA TATGGGGCTA 
GGTTTTCAAG GAGAGAAAGG AGTCAAGGGG GATGTTGGCC TCCCTGGCCC AGCAGGACCT 
CCACCATCTA CTGGAGAGCT GGAATTCATG GGATTCCCCA AAGGGAAGAA AGGATCCAAG 
GGTGAACCAG GGCCTAAGGG TTTTCCAGGC ATAAGTGGCC CTCCAGGCTT CCCGGGCCTT 
GGAACTACTG GAGAAAAGGG AGAAAAGGGA GAAAAGGGAA TCCCTGGTTT GCCAGGACCT 
AGGGGTCCCA TGGGTTCAGA AGGAGTCCAA GGCCCTCCAG GGCAACAGGG CAAGAAAGGG 
ACCCTGGGAT TTCCTGGGCT TAATGGATTC CAAGGAATTG AGGGTCAAAA GGGTGACATT 
GGCCTGCCAG GCCCAGATGT TTTCATCGAT ATAGATGGTG CTGTGATCTC AGGTAATCCT 
GGAGATCCTG GTGTACCTGG CCTCCCAGGC CTTAAAGGAG ATGAAGGCAT CCAAGGCCTA 
CGTGGCCCTT CTGGTGTCCC TGGATTGCCA GCATTATCAG GTGTCCCAGG AGCCCTAGGG 
CCTCAGGGAT TTCCAGGGCT GAAGGGGGAC CAAGGAAACC CAGGCCGTAC CACAATTGGA 
GCAGCTGGCC TCCCTGGCAG AGATGGTTTG CCAGGCCCAC CAGGTCCACC AGGCCCACCT 
AGTCCAGAAT TTGAGACTGA AACTCTACAC AACAAAGAGT CAGGGTTCCC TGGTCTCCGA 
GGAGAACAAG GTCCAAAAGG AAACCTAGGC CTCAAAGGAA TAAAAGGAGA CTCAGGTTTC 
TGTGCTTGTG ACGGTGGTGT TCCCAACACT GGACCACCCG GGGAACCAGG CCCACCTGGT 
CCATGGGGTC TCATAGGCCT TCCAGGCCTT AAAGGAGCCA GAGGAGATCG AGGCTCTGGG 
GGTGCACAGG GCCCAGCAGG GGCTCCAGGC TTAGTTGGGC CTCTGGGTCC TTCAGGACCC 
AAAGGAAAGA AGGGGGAACC AATTCTCAGT ACAATCCAAG GAATGCCAGG AGATCGGGGT 
GATTCTGGCT CCCAGGGCTT CCGTGGTGTA ATAGGAGAAC CAGGCAAGGA CGGAGTACCA 
GGTTTACCAG GTCTGCCAGG CCTTCCGGGT GATGGTGGAC AGGGCTTCCC AGGTGAAAAG 
GGGTTACCTG GACTTCCTGG TGAAAAAGGC CATCCTGGTC CACCTGGCCT CCCAGGAAAT 
GGGTTACCAG GACTTCCTGG ACCCCGTGGG CTTCCTGGAG ATAAAGGCAA GGATGGATTA 
CCGGGACAAC AAGGCCTTCC CGGATCTAAG GGAATCACCC TGCCCTGTAT TATTCCTGGG 
TCATACGGTC CATCAGGATT TCCAGGCACT CCCGGATTCC CAGGCCCTAA AGGGTCTCGA 
GGCCTCCCTG GGACCCCAGG CCAGCCTGGG TCAAGTGGAA GTAAAGGAGA GCCAGGGAGT 
CCAGGATTGG TTCATCTTCC TGAATTACCA GGATTTCCTG GACCTCGTGG GGAGAAGGGC 
TTGCCTGGGT TTCCTGGGCT CCCTGGAAAA GATGGCTTGC CTGGGATGAT TGGCAGTCCA 
GGCTTACCTG GTTCCAAGGG AGCCACTGGT GACATCTTTG GTGCTGAAAA TGGTGCTCCG 
GGGGAACAAG GCCTACAAGG ATTAACAGGG CACAAAGGAT TTCTTGGAGA CTCTGGCCTT 
CCAGGACTCA AGGGTGTGCA CGGGAAGCCT GGCTTACTAG GCCCCAAAGG TGAGCGGGGC 
AGCCCTGGGA CACCAGGACA GGTGGGACAG CCAGGCACCC CAGGATCTAG TGGTCCATAT 
GGCATCAAGG GCAAATCTGG GCTCCCAGGA GCACCAGGCT TCCCAGGCAT CTCAGGACAT 
CCTGGAAAGA AAGGAACAAG AGGCAAGAAA GGTCCTCCTG GATCAATTGT AAAGAAAGGG 
CTGCCAGGGC TAAAAGGCCT TCCTGGAAAT CCAGGCCTAG TAGGACTGAA AGGAAGCCCA 
GGCTCTCCAG GGGTCGCTGG GTTGCCAGCC CTCTCTGGAC CCAAGGGAGA GAAGGGGTCT 
GTTGGATTCG TAGGTTTTCC AGGAATACCA GGTCTGCCTG GTATTCCTGG AACAAGAGGA 
TTAAAAGGAA TTCCAGGATC AACTGGAAAA ATGGGACCAT CTGGACGTGC TGGTACTCCT 
GGTGAAAAGG GAGACAGAGG CAATCCGGGG CCAGTCGGAA TACCTAGTCC AAGACGTCCA 
ATGTCAAACC TTTGGCTCAA AGGAGACAAA GGCTCTCAAG GCTCAGCCGG ATCCAATGGA 
TTTCCTGGGC CAAGAGGTGA CAAAGGAGAG GCTGGTCGAC CTGGACCACC AGGCCTACCT 
GGAGCTCCTG GCCTCCCAGG CATTATCAAA GGAGTTAGTG GAAAGCCAGG GCCCCCTGGC 
TTCATGGGAA TCCGGGGCTT ACCTGGCCTG AAGGGGTCCT CTGGGATCAC AGGTTTCCCA 
GGAATGCCAG GAGAAAGTGG TTCACAAGGT ATCAGAGGGT CGCCTGGACT CCCAGGAGCA 
TCTGGTCTCC CAGGCCTGAA AGGAGACAAC GGCCAGACAG TTGAAATTTC CGGTAGCCCA 
GGACCCAAGG GACAGCCTGG CGAATCTGGT TTTAAAGGCA CAAAAGGAAG AGATGGACTA 
ATAGGCAATA TAGGCTTCCC TGGAAACAAA GGTGAAGATG GAAAAGTTGG TGTTTCTGGA 
GATGTTGGCC TTCCTGGAGC TCCAGGATTT CCAGGAGTTG CCGGCATGAG AGGAGAACCA 
GGACTTCCAG GTTCTTCTGG TCACCAAGGG GCAATTGGGC CTCTAGGATC CCCCGGATTA 
ATAGGACCCA AAGGACCTAG TATCACCGGT GTGCCTGGGC CTGCTGGTCT CCCTGGACCC 
AAAGGAGAAA AAGGATATCC AGGAATTGGC ATCGGAGCTC CAGGGAAGCC GGGCCTGAGA 
GGGCAAAAAG GTGATCGAGG TTTCCCAGGT CTCCAGGGCC CTGCTGGTCT CCCCGGTGCC 
CCAGGCATCT CCTTGCCCTC ACTCATAGCA GGACAGCCTG GTGACCCCGG GCGACCAGGC 
CTAGATGGAG AACGAGGCCG CCCAGGCCCC GCTGGACCCC CAGGTCCCCC TGGGCCATCC 
TCGAATCAAG GCGACACCGG AGACCCTGGC TTCCCTGGAA TTCCTGGACC TAAAGGGCCT 
AAGGGAGACC AAGGAATTCC AGGTTTTTCT GGCCTCCCTG GAGAGCTAGG ACTGAAAGGC 
ATGAGAGGTG AGCCTGGCTT CATGGGGACT CCAGGCAAGG TTGGGCCACC TGGAGACCCA 
GGATTTCCCG GAATGAAGGG GAAGGCAGGG CCAAGAGGCT CTTCTGGCCT CCAAGGTGAT 
CCTGGACAAA CACCAACTGC AGAAGCTGTC CAGGTTCCTC CTGGACCCTT GGGTCTACCA 
GGGATCGATG GCATCCCTGG CCTCACTGGG GACCCTGGGG CTCAAGGCCC TGTAGGCCTA 
CAAGGCTCCA AAGGTTTACC TGGCATCCCC GGTAAAGATG GCCCCAGTGG GCTCCCAGGC 
CCACCTGGGG CTCTTGGTGA TCCTGGTCTG CCTGGACTGC AAGGCCCTCC AGGATTTGAA 
GGAGCTCCAG GGCAGCAAGG CCCCTTCGGG ATGCCTGGAA TGCCTGGCCA GAGCATGAGA 
GTGGGCTACA CGTTGGTAAA GCACAGCCAG TCGGAACAGG TGCCCCCGTG TCCCATCGGG 
ATGAGCCAGC TGTGGGTGGG GTACAGCTTA CTGTTTGTGG AGGGGCAAGA GAAAGCCCAC 
AACCAGGACC TGGGCTTTGC TGGCTCCTGT CTGCCCCGCT TCAGCACCAT GCCCTTCATC 
TACTGCAACA TCAACGAGGT GTGCCACTAT GCCAGGCGCA ATGATAAATC TTACTGGCTC 
TCCACTACCG CCCCTATCCC CATGATGCCC GTCAGCCAGA CCCAGATTCC CCAGTACATC 
AGCCGCTGCT CTGTGTGTGA GGCACCCTCG CAAGCCATTG CTGTGCACAG CCAGGACATC 
ACCATCCCGC AGTGCCCCCT GGGCTGGCGC AGCCTCTGGA TTGGGTACTC TTTCCTCATG 
CACACTGCCG CTGGTGCCGA GGGTGGAGGC CAGTCCCTGG TCTCACCTGG CTCCTGCCTA 
GAGGACTTTC GGGCCACTCC TTTCATCGAA TGCAGTGGTG CCCGAGGCAC CTGCCACTAC 
TTTGCAAACA AGTACAGTTT CTGGTTGACC ACAGTGGAGG AGAGGCAGCA GTTTGGGGAG 
TTGCCTGTGT CTGAAACGCT GAAAGCTGGG CAGCTCCACA CTCGAGTCAG TCGCTGCCAG 
GTGTGTATGA AAAGCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( NM_001847.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001838.2 Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant A, mRNA.

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CloneID	OHu08685
Clone ID Related Accession (Same CDS sequence)	NM_001847.3 , NM_001847.4
Accession Version	NM_001847.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	collagen alpha-6(IV) chain isoform A precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL034369.1, U04845.1, AL136080.6, AL031177.1 and CB050016.1. On Dec 27, 2013 this sequence version replaced NM_001847.2. Transcript Variant: This variant (A) utilizes a different alternative exon in the 5' UTR and coding region, exon 1A, which results in use of an alternate upstream translation start, compared to variant 3. The resulting protein (isoform A) has a distinct and shorter N-terminus, compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U04845.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146982 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTTATAA ACAAGTTGTG GCTGCTCCTG GTTACGTTGT GCCTGACCGA GGAACTGGCA 
GCAGCGGGAG AGAAGTCTTA TGGAAAGCCA TGTGGGGGCC AGGACTGCAG TGGGAGCTGT 
CAGTGTTTTC CTGAGAAAGG AGCGAGAGGA CGACCTGGAC CAATTGGAAT TCAAGGCCCA 
ACAGGTCCTC AAGGATTCAC TGGCTCTACT GGTTTATCGG GATTGAAAGG AGAAAGGGGT 
TTCCCAGGCC TTCTGGGACC TTATGGACCA AAAGGAGATA AGGGTCCCAT GGGAGTTCCT 
GGCTTTCTTG GCATCAATGG GATTCCGGGC CACCCTGGAC AACCAGGCCC CAGAGGCCCA 
CCTGGTCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATCCTGGGC TTCTCGGACC ACCCGGGCTT CCTGGTCAGA AAGGATCAAA AGGTGACCCT 
GTCCTTGCTC CAGGTAGTTT CAAAGGAATG AAGGGGGATC CTGGGCTGCC TGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TTTCCTGGAG CTGTAGGACC TGCAGGACCA 
CCAGGATTAC AAGGTCCTCC AGGGCCTCCT GGTCCTCTTG GTCCTGATGG GAATATGGGG 
CTAGGTTTTC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCTGG CCCAGCAGGA 
CCTCCACCAT CTACTGGAGA GCTGGAATTC ATGGGATTCC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTAA GGGTTTTCCA GGCATAAGTG GCCCTCCAGG CTTCCCGGGC 
CTTGGAACTA CTGGAGAAAA GGGAGAAAAG GGAGAAAAGG GAATCCCTGG TTTGCCAGGA 
CCTAGGGGTC CCATGGGTTC AGAAGGAGTC CAAGGCCCTC CAGGGCAACA GGGCAAGAAA 
GGGACCCTGG GATTTCCTGG GCTTAATGGA TTCCAAGGAA TTGAGGGTCA AAAGGGTGAC 
ATTGGCCTGC CAGGCCCAGA TGTTTTCATC GATATAGATG GTGCTGTGAT CTCAGGTAAT 
CCTGGAGATC CTGGTGTACC TGGCCTCCCA GGCCTTAAAG GAGATGAAGG CATCCAAGGC 
CTACGTGGCC CTTCTGGTGT CCCTGGATTG CCAGCATTAT CAGGTGTCCC AGGAGCCCTA 
GGGCCTCAGG GATTTCCAGG GCTGAAGGGG GACCAAGGAA ACCCAGGCCG TACCACAATT 
GGAGCAGCTG GCCTCCCTGG CAGAGATGGT TTGCCAGGCC CACCAGGTCC ACCAGGCCCA 
CCTAGTCCAG AATTTGAGAC TGAAACTCTA CACAACAAAG AGTCAGGGTT CCCTGGTCTC 
CGAGGAGAAC AAGGTCCAAA AGGAAACCTA GGCCTCAAAG GAATAAAAGG AGACTCAGGT 
TTCTGTGCTT GTGACGGTGG TGTTCCCAAC ACTGGACCAC CCGGGGAACC AGGCCCACCT 
GGTCCATGGG GTCTCATAGG CCTTCCAGGC CTTAAAGGAG CCAGAGGAGA TCGAGGCTCT 
GGGGGTGCAC AGGGCCCAGC AGGGGCTCCA GGCTTAGTTG GGCCTCTGGG TCCTTCAGGA 
CCCAAAGGAA AGAAGGGGGA ACCAATTCTC AGTACAATCC AAGGAATGCC AGGAGATCGG 
GGTGATTCTG GCTCCCAGGG CTTCCGTGGT GTAATAGGAG AACCAGGCAA GGACGGAGTA 
CCAGGTTTAC CAGGTCTGCC AGGCCTTCCG GGTGATGGTG GACAGGGCTT CCCAGGTGAA 
AAGGGGTTAC CTGGACTTCC TGGTGAAAAA GGCCATCCTG GTCCACCTGG CCTCCCAGGA 
AATGGGTTAC CAGGACTTCC TGGACCCCGT GGGCTTCCTG GAGATAAAGG CAAGGATGGA 
TTACCGGGAC AACAAGGCCT TCCCGGATCT AAGGGAATCA CCCTGCCCTG TATTATTCCT 
GGGTCATACG GTCCATCAGG ATTTCCAGGC ACTCCCGGAT TCCCAGGCCC TAAAGGGTCT 
CGAGGCCTCC CTGGGACCCC AGGCCAGCCT GGGTCAAGTG GAAGTAAAGG AGAGCCAGGG 
AGTCCAGGAT TGGTTCATCT TCCTGAATTA CCAGGATTTC CTGGACCTCG TGGGGAGAAG 
GGCTTGCCTG GGTTTCCTGG GCTCCCTGGA AAAGATGGCT TGCCTGGGAT GATTGGCAGT 
CCAGGCTTAC CTGGTTCCAA GGGAGCCACT GGTGACATCT TTGGTGCTGA AAATGGTGCT 
CCGGGGGAAC AAGGCCTACA AGGATTAACA GGGCACAAAG GATTTCTTGG AGACTCTGGC 
CTTCCAGGAC TCAAGGGTGT GCACGGGAAG CCTGGCTTAC TAGGCCCCAA AGGTGAGCGG 
GGCAGCCCTG GGACACCAGG ACAGGTGGGA CAGCCAGGCA CCCCAGGATC TAGTGGTCCA 
TATGGCATCA AGGGCAAATC TGGGCTCCCA GGAGCACCAG GCTTCCCAGG CATCTCAGGA 
CATCCTGGAA AGAAAGGAAC AAGAGGCAAG AAAGGTCCTC CTGGATCAAT TGTAAAGAAA 
GGGCTGCCAG GGCTAAAAGG CCTTCCTGGA AATCCAGGCC TAGTAGGACT GAAAGGAAGC 
CCAGGCTCTC CAGGGGTCGC TGGGTTGCCA GCCCTCTCTG GACCCAAGGG AGAGAAGGGG 
TCTGTTGGAT TCGTAGGTTT TCCAGGAATA CCAGGTCTGC CTGGTATTCC TGGAACAAGA 
GGATTAAAAG GAATTCCAGG ATCAACTGGA AAAATGGGAC CATCTGGACG TGCTGGTACT 
CCTGGTGAAA AGGGAGACAG AGGCAATCCG GGGCCAGTCG GAATACCTAG TCCAAGACGT 
CCAATGTCAA ACCTTTGGCT CAAAGGAGAC AAAGGCTCTC AAGGCTCAGC CGGATCCAAT 
GGATTTCCTG GGCCAAGAGG TGACAAAGGA GAGGCTGGTC GACCTGGACC ACCAGGCCTA 
CCTGGAGCTC CTGGCCTCCC AGGCATTATC AAAGGAGTTA GTGGAAAGCC AGGGCCCCCT 
GGCTTCATGG GAATCCGGGG CTTACCTGGC CTGAAGGGGT CCTCTGGGAT CACAGGTTTC 
CCAGGAATGC CAGGAGAAAG TGGTTCACAA GGTATCAGAG GGTCGCCTGG ACTCCCAGGA 
GCATCTGGTC TCCCAGGCCT GAAAGGAGAC AACGGCCAGA CAGTTGAAAT TTCCGGTAGC 
CCAGGACCCA AGGGACAGCC TGGCGAATCT GGTTTTAAAG GCACAAAAGG AAGAGATGGA 
CTAATAGGCA ATATAGGCTT CCCTGGAAAC AAAGGTGAAG ATGGAAAAGT TGGTGTTTCT 
GGAGATGTTG GCCTTCCTGG AGCTCCAGGA TTTCCAGGAG TTGCCGGCAT GAGAGGAGAA 
CCAGGACTTC CAGGTTCTTC TGGTCACCAA GGGGCAATTG GGCCTCTAGG ATCCCCCGGA 
TTAATAGGAC CCAAAGGCTT CCCTGGATTT CCTGGTTTAC ATGGACTGAA TGGGCTTCCG 
GGCACCAAGG GTACCCATGG CACTCCAGGA CCTAGTATCA CCGGTGTGCC TGGGCCTGCT 
GGTCTCCCTG GACCCAAAGG AGAAAAAGGA TATCCAGGAA TTGGCATCGG AGCTCCAGGG 
AAGCCGGGCC TGAGAGGGCA AAAAGGTGAT CGAGGTTTCC CAGGTCTCCA GGGCCCTGCT 
GGTCTCCCCG GTGCCCCAGG CATCTCCTTG CCCTCACTCA TAGCAGGACA GCCTGGTGAC 
CCCGGGCGAC CAGGCCTAGA TGGAGAACGA GGCCGCCCAG GCCCCGCTGG ACCCCCAGGT 
CCCCCTGGGC CATCCTCGAA TCAAGGCGAC ACCGGAGACC CTGGCTTCCC TGGAATTCCT 
GGACCTAAAG GGCCTAAGGG AGACCAAGGA ATTCCAGGTT TTTCTGGCCT CCCTGGAGAG 
CTAGGACTGA AAGGCATGAG AGGTGAGCCT GGCTTCATGG GGACTCCAGG CAAGGTTGGG 
CCACCTGGAG ACCCAGGATT TCCCGGAATG AAGGGGAAGG CAGGGCCAAG AGGCTCTTCT 
GGCCTCCAAG GTGATCCTGG ACAAACACCA ACTGCAGAAG CTGTCCAGGT TCCTCCTGGA 
CCCTTGGGTC TACCAGGGAT CGATGGCATC CCTGGCCTCA CTGGGGACCC TGGGGCTCAA 
GGCCCTGTAG GCCTACAAGG CTCCAAAGGT TTACCTGGCA TCCCCGGTAA AGATGGCCCC 
AGTGGGCTCC CAGGCCCACC TGGGGCTCTT GGTGATCCTG GTCTGCCTGG ACTGCAAGGC 
CCTCCAGGAT TTGAAGGAGC TCCAGGGCAG CAAGGCCCCT TCGGGATGCC TGGAATGCCT 
GGCCAGAGCA TGAGAGTGGG CTACACGTTG GTAAAGCACA GCCAGTCGGA ACAGGTGCCC 
CCGTGTCCCA TCGGGATGAG CCAGCTGTGG GTGGGGTACA GCTTACTGTT TGTGGAGGGG 
CAAGAGAAAG CCCACAACCA GGACCTGGGC TTTGCTGGCT CCTGTCTGCC CCGCTTCAGC 
ACCATGCCCT TCATCTACTG CAACATCAAC GAGGTGTGCC ACTATGCCAG GCGCAATGAT 
AAATCTTACT GGCTCTCCAC TACCGCCCCT ATCCCCATGA TGCCCGTCAG CCAGACCCAG 
ATTCCCCAGT ACATCAGCCG CTGCTCTGTG TGTGAGGCAC CCTCGCAAGC CATTGCTGTG 
CACAGCCAGG ACATCACCAT CCCGCAGTGC CCCCTGGGCT GGCGCAGCCT CTGGATTGGG 
TACTCTTTCC TCATGCACAC TGCCGCTGGT GCCGAGGGTG GAGGCCAGTC CCTGGTCTCA 
CCTGGCTCCT GCCTAGAGGA CTTTCGGGCC ACTCCTTTCA TCGAATGCAG TGGTGCCCGA 
GGCACCTGCC ACTACTTTGC AAACAAGTAC AGTTTCTGGT TGACCACAGT GGAGGAGAGG 
CAGCAGTTTG GGGAGTTGCC TGTGTCTGAA ACGCTGAAAG CTGGGCAGCT CCACACTCGA 
GTCAGTCGCT GCCAGGTGTG TATGAAAAGC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001838.2
CDS	127..5202
Translation	MLINKLWLLLVTLCLTEELAAAGEKSYGKPCGGQDCSGSCQCFPEKGARGRPGPIGIQGPTGPQGFTGSTGLSGLKGERGFPGLLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYPGLLGPPGLPGQKGSKGDPVLAPGSFKGMKGDPGLPGLDGITGPQGAPGFPGAVGPAGPPGLQGPPGPPGPLGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPKGFPGISGPPGFPGLGTTGEKGEKGEKGIPGLPGPRGPMGSEGVQGPPGQQGKKGTLGFPGLNGFQGIEGQKGDIGLPGPDVFIDIDGAVISGNPGDPGVPGLPGLKGDEGIQGLRGPSGVPGLPALSGVPGALGPQGFPGLKGDQGNPGRTTIGAAGLPGRDGLPGPPGPPGPPSPEFETETLHNKESGFPGLRGEQGPKGNLGLKGIKGDSGFCACDGGVPNTGPPGEPGPPGPWGLIGLPGLKGARGDRGSGGAQGPAGAPGLVGPLGPSGPKGKKGEPILSTIQGMPGDRGDSGSQGFRGVIGEPGKDGVPGLPGLPGLPGDGGQGFPGEKGLPGLPGEKGHPGPPGLPGNGLPGLPGPRGLPGDKGKDGLPGQQGLPGSKGITLPCIIPGSYGPSGFPGTPGFPGPKGSRGLPGTPGQPGSSGSKGEPGSPGLVHLPELPGFPGPRGEKGLPGFPGLPGKDGLPGMIGSPGLPGSKGATGDIFGAENGAPGEQGLQGLTGHKGFLGDSGLPGLKGVHGKPGLLGPKGERGSPGTPGQVGQPGTPGSSGPYGIKGKSGLPGAPGFPGISGHPGKKGTRGKKGPPGSIVKKGLPGLKGLPGNPGLVGLKGSPGSPGVAGLPALSGPKGEKGSVGFVGFPGIPGLPGIPGTRGLKGIPGSTGKMGPSGRAGTPGEKGDRGNPGPVGIPSPRRPMSNLWLKGDKGSQGSAGSNGFPGPRGDKGEAGRPGPPGLPGAPGLPGIIKGVSGKPGPPGFMGIRGLPGLKGSSGITGFPGMPGESGSQGIRGSPGLPGASGLPGLKGDNGQTVEISGSPGPKGQPGESGFKGTKGRDGLIGNIGFPGNKGEDGKVGVSGDVGLPGAPGFPGVAGMRGEPGLPGSSGHQGAIGPLGSPGLIGPKGFPGFPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGYPGIGIGAPGKPGLRGQKGDRGFPGLQGPAGLPGAPGISLPSLIAGQPGDPGRPGLDGERGRPGPAGPPGPPGPSSNQGDTGDPGFPGIPGPKGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGFPGMKGKAGPRGSSGLQGDPGQTPTAEAVQVPPGPLGLPGIDGIPGLTGDPGAQGPVGLQGSKGLPGIPGKDGPSGLPGPPGALGDPGLPGLQGPPGFEGAPGQQGPFGMPGMPGQSMRVGYTLVKHSQSEQVPPCPIGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVSQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGELPVSETLKAGQLHTRVSRCQVCMKSL

Target ORF information:

RefSeq Version	NM_001847.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001847.3

ORF Insert Sequence:

ATGCTTATAA ACAAGTTGTG GCTGCTCCTG GTTACGTTGT GCCTGACCGA GGAACTGGCA 
GCAGCGGGAG AGAAGTCTTA TGGAAAGCCA TGTGGGGGCC AGGACTGCAG TGGGAGCTGT 
CAGTGTTTTC CTGAGAAAGG AGCGAGAGGA CGACCTGGAC CAATTGGAAT TCAAGGCCCA 
ACAGGTCCTC AAGGATTCAC TGGCTCTACT GGTTTATCGG GATTGAAAGG AGAAAGGGGT 
TTCCCAGGCC TTCTGGGACC TTATGGACCA AAAGGAGATA AGGGTCCCAT GGGAGTTCCT 
GGCTTTCTTG GCATCAATGG GATTCCGGGC CACCCTGGAC AACCAGGCCC CAGAGGCCCA 
CCTGGTCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATCCTGGGC TTCTCGGACC ACCCGGGCTT CCTGGTCAGA AAGGATCAAA AGGTGACCCT 
GTCCTTGCTC CAGGTAGTTT CAAAGGAATG AAGGGGGATC CTGGGCTGCC TGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TTTCCTGGAG CTGTAGGACC TGCAGGACCA 
CCAGGATTAC AAGGTCCTCC AGGGCCTCCT GGTCCTCTTG GTCCTGATGG GAATATGGGG 
CTAGGTTTTC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCTGG CCCAGCAGGA 
CCTCCACCAT CTACTGGAGA GCTGGAATTC ATGGGATTCC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTAA GGGTTTTCCA GGCATAAGTG GCCCTCCAGG CTTCCCGGGC 
CTTGGAACTA CTGGAGAAAA GGGAGAAAAG GGAGAAAAGG GAATCCCTGG TTTGCCAGGA 
CCTAGGGGTC CCATGGGTTC AGAAGGAGTC CAAGGCCCTC CAGGGCAACA GGGCAAGAAA 
GGGACCCTGG GATTTCCTGG GCTTAATGGA TTCCAAGGAA TTGAGGGTCA AAAGGGTGAC 
ATTGGCCTGC CAGGCCCAGA TGTTTTCATC GATATAGATG GTGCTGTGAT CTCAGGTAAT 
CCTGGAGATC CTGGTGTACC TGGCCTCCCA GGCCTTAAAG GAGATGAAGG CATCCAAGGC 
CTACGTGGCC CTTCTGGTGT CCCTGGATTG CCAGCATTAT CAGGTGTCCC AGGAGCCCTA 
GGGCCTCAGG GATTTCCAGG GCTGAAGGGG GACCAAGGAA ACCCAGGCCG TACCACAATT 
GGAGCAGCTG GCCTCCCTGG CAGAGATGGT TTGCCAGGCC CACCAGGTCC ACCAGGCCCA 
CCTAGTCCAG AATTTGAGAC TGAAACTCTA CACAACAAAG AGTCAGGGTT CCCTGGTCTC 
CGAGGAGAAC AAGGTCCAAA AGGAAACCTA GGCCTCAAAG GAATAAAAGG AGACTCAGGT 
TTCTGTGCTT GTGACGGTGG TGTTCCCAAC ACTGGACCAC CCGGGGAACC AGGCCCACCT 
GGTCCATGGG GTCTCATAGG CCTTCCAGGC CTTAAAGGAG CCAGAGGAGA TCGAGGCTCT 
GGGGGTGCAC AGGGCCCAGC AGGGGCTCCA GGCTTAGTTG GGCCTCTGGG TCCTTCAGGA 
CCCAAAGGAA AGAAGGGGGA ACCAATTCTC AGTACAATCC AAGGAATGCC AGGAGATCGG 
GGTGATTCTG GCTCCCAGGG CTTCCGTGGT GTAATAGGAG AACCAGGCAA GGACGGAGTA 
CCAGGTTTAC CAGGTCTGCC AGGCCTTCCG GGTGATGGTG GACAGGGCTT CCCAGGTGAA 
AAGGGGTTAC CTGGACTTCC TGGTGAAAAA GGCCATCCTG GTCCACCTGG CCTCCCAGGA 
AATGGGTTAC CAGGACTTCC TGGACCCCGT GGGCTTCCTG GAGATAAAGG CAAGGATGGA 
TTACCGGGAC AACAAGGCCT TCCCGGATCT AAGGGAATCA CCCTGCCCTG TATTATTCCT 
GGGTCATACG GTCCATCAGG ATTTCCAGGC ACTCCCGGAT TCCCAGGCCC TAAAGGGTCT 
CGAGGCCTCC CTGGGACCCC AGGCCAGCCT GGGTCAAGTG GAAGTAAAGG AGAGCCAGGG 
AGTCCAGGAT TGGTTCATCT TCCTGAATTA CCAGGATTTC CTGGACCTCG TGGGGAGAAG 
GGCTTGCCTG GGTTTCCTGG GCTCCCTGGA AAAGATGGCT TGCCTGGGAT GATTGGCAGT 
CCAGGCTTAC CTGGTTCCAA GGGAGCCACT GGTGACATCT TTGGTGCTGA AAATGGTGCT 
CCGGGGGAAC AAGGCCTACA AGGATTAACA GGGCACAAAG GATTTCTTGG AGACTCTGGC 
CTTCCAGGAC TCAAGGGTGT GCACGGGAAG CCTGGCTTAC TAGGCCCCAA AGGTGAGCGG 
GGCAGCCCTG GGACACCAGG ACAGGTGGGA CAGCCAGGCA CCCCAGGATC TAGTGGTCCA 
TATGGCATCA AGGGCAAATC TGGGCTCCCA GGAGCACCAG GCTTCCCAGG CATCTCAGGA 
CATCCTGGAA AGAAAGGAAC AAGAGGCAAG AAAGGTCCTC CTGGATCAAT TGTAAAGAAA 
GGGCTGCCAG GGCTAAAAGG CCTTCCTGGA AATCCAGGCC TAGTAGGACT GAAAGGAAGC 
CCAGGCTCTC CAGGGGTCGC TGGGTTGCCA GCCCTCTCTG GACCCAAGGG AGAGAAGGGG 
TCTGTTGGAT TCGTAGGTTT TCCAGGAATA CCAGGTCTGC CTGGTATTCC TGGAACAAGA 
GGATTAAAAG GAATTCCAGG ATCAACTGGA AAAATGGGAC CATCTGGACG TGCTGGTACT 
CCTGGTGAAA AGGGAGACAG AGGCAATCCG GGGCCAGTCG GAATACCTAG TCCAAGACGT 
CCAATGTCAA ACCTTTGGCT CAAAGGAGAC AAAGGCTCTC AAGGCTCAGC CGGATCCAAT 
GGATTTCCTG GGCCAAGAGG TGACAAAGGA GAGGCTGGTC GACCTGGACC ACCAGGCCTA 
CCTGGAGCTC CTGGCCTCCC AGGCATTATC AAAGGAGTTA GTGGAAAGCC AGGGCCCCCT 
GGCTTCATGG GAATCCGGGG CTTACCTGGC CTGAAGGGGT CCTCTGGGAT CACAGGTTTC 
CCAGGAATGC CAGGAGAAAG TGGTTCACAA GGTATCAGAG GGTCGCCTGG ACTCCCAGGA 
GCATCTGGTC TCCCAGGCCT GAAAGGAGAC AACGGCCAGA CAGTTGAAAT TTCCGGTAGC 
CCAGGACCCA AGGGACAGCC TGGCGAATCT GGTTTTAAAG GCACAAAAGG AAGAGATGGA 
CTAATAGGCA ATATAGGCTT CCCTGGAAAC AAAGGTGAAG ATGGAAAAGT TGGTGTTTCT 
GGAGATGTTG GCCTTCCTGG AGCTCCAGGA TTTCCAGGAG TTGCCGGCAT GAGAGGAGAA 
CCAGGACTTC CAGGTTCTTC TGGTCACCAA GGGGCAATTG GGCCTCTAGG ATCCCCCGGA 
TTAATAGGAC CCAAAGGCTT CCCTGGATTT CCTGGTTTAC ATGGACTGAA TGGGCTTCCG 
GGCACCAAGG GTACCCATGG CACTCCAGGA CCTAGTATCA CCGGTGTGCC TGGGCCTGCT 
GGTCTCCCTG GACCCAAAGG AGAAAAAGGA TATCCAGGAA TTGGCATCGG AGCTCCAGGG 
AAGCCGGGCC TGAGAGGGCA AAAAGGTGAT CGAGGTTTCC CAGGTCTCCA GGGCCCTGCT 
GGTCTCCCCG GTGCCCCAGG CATCTCCTTG CCCTCACTCA TAGCAGGACA GCCTGGTGAC 
CCCGGGCGAC CAGGCCTAGA TGGAGAACGA GGCCGCCCAG GCCCCGCTGG ACCCCCAGGT 
CCCCCTGGGC CATCCTCGAA TCAAGGCGAC ACCGGAGACC CTGGCTTCCC TGGAATTCCT 
GGACCTAAAG GGCCTAAGGG AGACCAAGGA ATTCCAGGTT TTTCTGGCCT CCCTGGAGAG 
CTAGGACTGA AAGGCATGAG AGGTGAGCCT GGCTTCATGG GGACTCCAGG CAAGGTTGGG 
CCACCTGGAG ACCCAGGATT TCCCGGAATG AAGGGGAAGG CAGGGCCAAG AGGCTCTTCT 
GGCCTCCAAG GTGATCCTGG ACAAACACCA ACTGCAGAAG CTGTCCAGGT TCCTCCTGGA 
CCCTTGGGTC TACCAGGGAT CGATGGCATC CCTGGCCTCA CTGGGGACCC TGGGGCTCAA 
GGCCCTGTAG GCCTACAAGG CTCCAAAGGT TTACCTGGCA TCCCCGGTAA AGATGGCCCC 
AGTGGGCTCC CAGGCCCACC TGGGGCTCTT GGTGATCCTG GTCTGCCTGG ACTGCAAGGC 
CCTCCAGGAT TTGAAGGAGC TCCAGGGCAG CAAGGCCCCT TCGGGATGCC TGGAATGCCT 
GGCCAGAGCA TGAGAGTGGG CTACACGTTG GTAAAGCACA GCCAGTCGGA ACAGGTGCCC 
CCGTGTCCCA TCGGGATGAG CCAGCTGTGG GTGGGGTACA GCTTACTGTT TGTGGAGGGG 
CAAGAGAAAG CCCACAACCA GGACCTGGGC TTTGCTGGCT CCTGTCTGCC CCGCTTCAGC 
ACCATGCCCT TCATCTACTG CAACATCAAC GAGGTGTGCC ACTATGCCAG GCGCAATGAT 
AAATCTTACT GGCTCTCCAC TACCGCCCCT ATCCCCATGA TGCCCGTCAG CCAGACCCAG 
ATTCCCCAGT ACATCAGCCG CTGCTCTGTG TGTGAGGCAC CCTCGCAAGC CATTGCTGTG 
CACAGCCAGG ACATCACCAT CCCGCAGTGC CCCCTGGGCT GGCGCAGCCT CTGGATTGGG 
TACTCTTTCC TCATGCACAC TGCCGCTGGT GCCGAGGGTG GAGGCCAGTC CCTGGTCTCA 
CCTGGCTCCT GCCTAGAGGA CTTTCGGGCC ACTCCTTTCA TCGAATGCAG TGGTGCCCGA 
GGCACCTGCC ACTACTTTGC AAACAAGTAC AGTTTCTGGT TGACCACAGT GGAGGAGAGG 
CAGCAGTTTG GGGAGTTGCC TGTGTCTGAA ACGCTGAAAG CTGGGCAGCT CCACACTCGA 
GTCAGTCGCT GCCAGGTGTG TATGAAAAGC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( NM_001847.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001838.2 Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant A, mRNA.

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CloneID	OHu08685
Clone ID Related Accession (Same CDS sequence)	NM_001847.3 , NM_001847.4
Accession Version	NM_001847.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	collagen alpha-6(IV) chain isoform A precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U04845.1, AL136080.6, AL031177.1 and CB050016.1. On Jun 2, 2019 this sequence version replaced NM_001847.3. Transcript Variant: This variant (A) utilizes a different alternative exon in the 5' UTR and coding region, exon 1A, which results in use of an alternate upstream translation start, compared to variant 3. The resulting protein (isoform A) has a distinct and shorter N-terminus, compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U04845.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146982 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTTATAA ACAAGTTGTG GCTGCTCCTG GTTACGTTGT GCCTGACCGA GGAACTGGCA 
GCAGCGGGAG AGAAGTCTTA TGGAAAGCCA TGTGGGGGCC AGGACTGCAG TGGGAGCTGT 
CAGTGTTTTC CTGAGAAAGG AGCGAGAGGA CGACCTGGAC CAATTGGAAT TCAAGGCCCA 
ACAGGTCCTC AAGGATTCAC TGGCTCTACT GGTTTATCGG GATTGAAAGG AGAAAGGGGT 
TTCCCAGGCC TTCTGGGACC TTATGGACCA AAAGGAGATA AGGGTCCCAT GGGAGTTCCT 
GGCTTTCTTG GCATCAATGG GATTCCGGGC CACCCTGGAC AACCAGGCCC CAGAGGCCCA 
CCTGGTCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATCCTGGGC TTCTCGGACC ACCCGGGCTT CCTGGTCAGA AAGGATCAAA AGGTGACCCT 
GTCCTTGCTC CAGGTAGTTT CAAAGGAATG AAGGGGGATC CTGGGCTGCC TGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TTTCCTGGAG CTGTAGGACC TGCAGGACCA 
CCAGGATTAC AAGGTCCTCC AGGGCCTCCT GGTCCTCTTG GTCCTGATGG GAATATGGGG 
CTAGGTTTTC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCTGG CCCAGCAGGA 
CCTCCACCAT CTACTGGAGA GCTGGAATTC ATGGGATTCC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTAA GGGTTTTCCA GGCATAAGTG GCCCTCCAGG CTTCCCGGGC 
CTTGGAACTA CTGGAGAAAA GGGAGAAAAG GGAGAAAAGG GAATCCCTGG TTTGCCAGGA 
CCTAGGGGTC CCATGGGTTC AGAAGGAGTC CAAGGCCCTC CAGGGCAACA GGGCAAGAAA 
GGGACCCTGG GATTTCCTGG GCTTAATGGA TTCCAAGGAA TTGAGGGTCA AAAGGGTGAC 
ATTGGCCTGC CAGGCCCAGA TGTTTTCATC GATATAGATG GTGCTGTGAT CTCAGGTAAT 
CCTGGAGATC CTGGTGTACC TGGCCTCCCA GGCCTTAAAG GAGATGAAGG CATCCAAGGC 
CTACGTGGCC CTTCTGGTGT CCCTGGATTG CCAGCATTAT CAGGTGTCCC AGGAGCCCTA 
GGGCCTCAGG GATTTCCAGG GCTGAAGGGG GACCAAGGAA ACCCAGGCCG TACCACAATT 
GGAGCAGCTG GCCTCCCTGG CAGAGATGGT TTGCCAGGCC CACCAGGTCC ACCAGGCCCA 
CCTAGTCCAG AATTTGAGAC TGAAACTCTA CACAACAAAG AGTCAGGGTT CCCTGGTCTC 
CGAGGAGAAC AAGGTCCAAA AGGAAACCTA GGCCTCAAAG GAATAAAAGG AGACTCAGGT 
TTCTGTGCTT GTGACGGTGG TGTTCCCAAC ACTGGACCAC CCGGGGAACC AGGCCCACCT 
GGTCCATGGG GTCTCATAGG CCTTCCAGGC CTTAAAGGAG CCAGAGGAGA TCGAGGCTCT 
GGGGGTGCAC AGGGCCCAGC AGGGGCTCCA GGCTTAGTTG GGCCTCTGGG TCCTTCAGGA 
CCCAAAGGAA AGAAGGGGGA ACCAATTCTC AGTACAATCC AAGGAATGCC AGGAGATCGG 
GGTGATTCTG GCTCCCAGGG CTTCCGTGGT GTAATAGGAG AACCAGGCAA GGACGGAGTA 
CCAGGTTTAC CAGGTCTGCC AGGCCTTCCG GGTGATGGTG GACAGGGCTT CCCAGGTGAA 
AAGGGGTTAC CTGGACTTCC TGGTGAAAAA GGCCATCCTG GTCCACCTGG CCTCCCAGGA 
AATGGGTTAC CAGGACTTCC TGGACCCCGT GGGCTTCCTG GAGATAAAGG CAAGGATGGA 
TTACCGGGAC AACAAGGCCT TCCCGGATCT AAGGGAATCA CCCTGCCCTG TATTATTCCT 
GGGTCATACG GTCCATCAGG ATTTCCAGGC ACTCCCGGAT TCCCAGGCCC TAAAGGGTCT 
CGAGGCCTCC CTGGGACCCC AGGCCAGCCT GGGTCAAGTG GAAGTAAAGG AGAGCCAGGG 
AGTCCAGGAT TGGTTCATCT TCCTGAATTA CCAGGATTTC CTGGACCTCG TGGGGAGAAG 
GGCTTGCCTG GGTTTCCTGG GCTCCCTGGA AAAGATGGCT TGCCTGGGAT GATTGGCAGT 
CCAGGCTTAC CTGGTTCCAA GGGAGCCACT GGTGACATCT TTGGTGCTGA AAATGGTGCT 
CCGGGGGAAC AAGGCCTACA AGGATTAACA GGGCACAAAG GATTTCTTGG AGACTCTGGC 
CTTCCAGGAC TCAAGGGTGT GCACGGGAAG CCTGGCTTAC TAGGCCCCAA AGGTGAGCGG 
GGCAGCCCTG GGACACCAGG ACAGGTGGGA CAGCCAGGCA CCCCAGGATC TAGTGGTCCA 
TATGGCATCA AGGGCAAATC TGGGCTCCCA GGAGCACCAG GCTTCCCAGG CATCTCAGGA 
CATCCTGGAA AGAAAGGAAC AAGAGGCAAG AAAGGTCCTC CTGGATCAAT TGTAAAGAAA 
GGGCTGCCAG GGCTAAAAGG CCTTCCTGGA AATCCAGGCC TAGTAGGACT GAAAGGAAGC 
CCAGGCTCTC CAGGGGTCGC TGGGTTGCCA GCCCTCTCTG GACCCAAGGG AGAGAAGGGG 
TCTGTTGGAT TCGTAGGTTT TCCAGGAATA CCAGGTCTGC CTGGTATTCC TGGAACAAGA 
GGATTAAAAG GAATTCCAGG ATCAACTGGA AAAATGGGAC CATCTGGACG TGCTGGTACT 
CCTGGTGAAA AGGGAGACAG AGGCAATCCG GGGCCAGTCG GAATACCTAG TCCAAGACGT 
CCAATGTCAA ACCTTTGGCT CAAAGGAGAC AAAGGCTCTC AAGGCTCAGC CGGATCCAAT 
GGATTTCCTG GGCCAAGAGG TGACAAAGGA GAGGCTGGTC GACCTGGACC ACCAGGCCTA 
CCTGGAGCTC CTGGCCTCCC AGGCATTATC AAAGGAGTTA GTGGAAAGCC AGGGCCCCCT 
GGCTTCATGG GAATCCGGGG CTTACCTGGC CTGAAGGGGT CCTCTGGGAT CACAGGTTTC 
CCAGGAATGC CAGGAGAAAG TGGTTCACAA GGTATCAGAG GGTCGCCTGG ACTCCCAGGA 
GCATCTGGTC TCCCAGGCCT GAAAGGAGAC AACGGCCAGA CAGTTGAAAT TTCCGGTAGC 
CCAGGACCCA AGGGACAGCC TGGCGAATCT GGTTTTAAAG GCACAAAAGG AAGAGATGGA 
CTAATAGGCA ATATAGGCTT CCCTGGAAAC AAAGGTGAAG ATGGAAAAGT TGGTGTTTCT 
GGAGATGTTG GCCTTCCTGG AGCTCCAGGA TTTCCAGGAG TTGCCGGCAT GAGAGGAGAA 
CCAGGACTTC CAGGTTCTTC TGGTCACCAA GGGGCAATTG GGCCTCTAGG ATCCCCCGGA 
TTAATAGGAC CCAAAGGCTT CCCTGGATTT CCTGGTTTAC ATGGACTGAA TGGGCTTCCG 
GGCACCAAGG GTACCCATGG CACTCCAGGA CCTAGTATCA CCGGTGTGCC TGGGCCTGCT 
GGTCTCCCTG GACCCAAAGG AGAAAAAGGA TATCCAGGAA TTGGCATCGG AGCTCCAGGG 
AAGCCGGGCC TGAGAGGGCA AAAAGGTGAT CGAGGTTTCC CAGGTCTCCA GGGCCCTGCT 
GGTCTCCCCG GTGCCCCAGG CATCTCCTTG CCCTCACTCA TAGCAGGACA GCCTGGTGAC 
CCCGGGCGAC CAGGCCTAGA TGGAGAACGA GGCCGCCCAG GCCCCGCTGG ACCCCCAGGT 
CCCCCTGGGC CATCCTCGAA TCAAGGCGAC ACCGGAGACC CTGGCTTCCC TGGAATTCCT 
GGACCTAAAG GGCCTAAGGG AGACCAAGGA ATTCCAGGTT TTTCTGGCCT CCCTGGAGAG 
CTAGGACTGA AAGGCATGAG AGGTGAGCCT GGCTTCATGG GGACTCCAGG CAAGGTTGGG 
CCACCTGGAG ACCCAGGATT TCCCGGAATG AAGGGGAAGG CAGGGCCAAG AGGCTCTTCT 
GGCCTCCAAG GTGATCCTGG ACAAACACCA ACTGCAGAAG CTGTCCAGGT TCCTCCTGGA 
CCCTTGGGTC TACCAGGGAT CGATGGCATC CCTGGCCTCA CTGGGGACCC TGGGGCTCAA 
GGCCCTGTAG GCCTACAAGG CTCCAAAGGT TTACCTGGCA TCCCCGGTAA AGATGGCCCC 
AGTGGGCTCC CAGGCCCACC TGGGGCTCTT GGTGATCCTG GTCTGCCTGG ACTGCAAGGC 
CCTCCAGGAT TTGAAGGAGC TCCAGGGCAG CAAGGCCCCT TCGGGATGCC TGGAATGCCT 
GGCCAGAGCA TGAGAGTGGG CTACACGTTG GTAAAGCACA GCCAGTCGGA ACAGGTGCCC 
CCGTGTCCCA TCGGGATGAG CCAGCTGTGG GTGGGGTACA GCTTACTGTT TGTGGAGGGG 
CAAGAGAAAG CCCACAACCA GGACCTGGGC TTTGCTGGCT CCTGTCTGCC CCGCTTCAGC 
ACCATGCCCT TCATCTACTG CAACATCAAC GAGGTGTGCC ACTATGCCAG GCGCAATGAT 
AAATCTTACT GGCTCTCCAC TACCGCCCCT ATCCCCATGA TGCCCGTCAG CCAGACCCAG 
ATTCCCCAGT ACATCAGCCG CTGCTCTGTG TGTGAGGCAC CCTCGCAAGC CATTGCTGTG 
CACAGCCAGG ACATCACCAT CCCGCAGTGC CCCCTGGGCT GGCGCAGCCT CTGGATTGGG 
TACTCTTTCC TCATGCACAC TGCCGCTGGT GCCGAGGGTG GAGGCCAGTC CCTGGTCTCA 
CCTGGCTCCT GCCTAGAGGA CTTTCGGGCC ACTCCTTTCA TCGAATGCAG TGGTGCCCGA 
GGCACCTGCC ACTACTTTGC AAACAAGTAC AGTTTCTGGT TGACCACAGT GGAGGAGAGG 
CAGCAGTTTG GGGAGTTGCC TGTGTCTGAA ACGCTGAAAG CTGGGCAGCT CCACACTCGA 
GTCAGTCGCT GCCAGGTGTG TATGAAAAGC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001838.2
CDS	88..5163
Translation	MLINKLWLLLVTLCLTEELAAAGEKSYGKPCGGQDCSGSCQCFPEKGARGRPGPIGIQGPTGPQGFTGSTGLSGLKGERGFPGLLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYPGLLGPPGLPGQKGSKGDPVLAPGSFKGMKGDPGLPGLDGITGPQGAPGFPGAVGPAGPPGLQGPPGPPGPLGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPKGFPGISGPPGFPGLGTTGEKGEKGEKGIPGLPGPRGPMGSEGVQGPPGQQGKKGTLGFPGLNGFQGIEGQKGDIGLPGPDVFIDIDGAVISGNPGDPGVPGLPGLKGDEGIQGLRGPSGVPGLPALSGVPGALGPQGFPGLKGDQGNPGRTTIGAAGLPGRDGLPGPPGPPGPPSPEFETETLHNKESGFPGLRGEQGPKGNLGLKGIKGDSGFCACDGGVPNTGPPGEPGPPGPWGLIGLPGLKGARGDRGSGGAQGPAGAPGLVGPLGPSGPKGKKGEPILSTIQGMPGDRGDSGSQGFRGVIGEPGKDGVPGLPGLPGLPGDGGQGFPGEKGLPGLPGEKGHPGPPGLPGNGLPGLPGPRGLPGDKGKDGLPGQQGLPGSKGITLPCIIPGSYGPSGFPGTPGFPGPKGSRGLPGTPGQPGSSGSKGEPGSPGLVHLPELPGFPGPRGEKGLPGFPGLPGKDGLPGMIGSPGLPGSKGATGDIFGAENGAPGEQGLQGLTGHKGFLGDSGLPGLKGVHGKPGLLGPKGERGSPGTPGQVGQPGTPGSSGPYGIKGKSGLPGAPGFPGISGHPGKKGTRGKKGPPGSIVKKGLPGLKGLPGNPGLVGLKGSPGSPGVAGLPALSGPKGEKGSVGFVGFPGIPGLPGIPGTRGLKGIPGSTGKMGPSGRAGTPGEKGDRGNPGPVGIPSPRRPMSNLWLKGDKGSQGSAGSNGFPGPRGDKGEAGRPGPPGLPGAPGLPGIIKGVSGKPGPPGFMGIRGLPGLKGSSGITGFPGMPGESGSQGIRGSPGLPGASGLPGLKGDNGQTVEISGSPGPKGQPGESGFKGTKGRDGLIGNIGFPGNKGEDGKVGVSGDVGLPGAPGFPGVAGMRGEPGLPGSSGHQGAIGPLGSPGLIGPKGFPGFPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGYPGIGIGAPGKPGLRGQKGDRGFPGLQGPAGLPGAPGISLPSLIAGQPGDPGRPGLDGERGRPGPAGPPGPPGPSSNQGDTGDPGFPGIPGPKGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGFPGMKGKAGPRGSSGLQGDPGQTPTAEAVQVPPGPLGLPGIDGIPGLTGDPGAQGPVGLQGSKGLPGIPGKDGPSGLPGPPGALGDPGLPGLQGPPGFEGAPGQQGPFGMPGMPGQSMRVGYTLVKHSQSEQVPPCPIGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVSQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGELPVSETLKAGQLHTRVSRCQVCMKSL

Target ORF information:

RefSeq Version	NM_001847.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001847.4

ORF Insert Sequence:

ATGCTTATAA ACAAGTTGTG GCTGCTCCTG GTTACGTTGT GCCTGACCGA GGAACTGGCA 
GCAGCGGGAG AGAAGTCTTA TGGAAAGCCA TGTGGGGGCC AGGACTGCAG TGGGAGCTGT 
CAGTGTTTTC CTGAGAAAGG AGCGAGAGGA CGACCTGGAC CAATTGGAAT TCAAGGCCCA 
ACAGGTCCTC AAGGATTCAC TGGCTCTACT GGTTTATCGG GATTGAAAGG AGAAAGGGGT 
TTCCCAGGCC TTCTGGGACC TTATGGACCA AAAGGAGATA AGGGTCCCAT GGGAGTTCCT 
GGCTTTCTTG GCATCAATGG GATTCCGGGC CACCCTGGAC AACCAGGCCC CAGAGGCCCA 
CCTGGTCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATCCTGGGC TTCTCGGACC ACCCGGGCTT CCTGGTCAGA AAGGATCAAA AGGTGACCCT 
GTCCTTGCTC CAGGTAGTTT CAAAGGAATG AAGGGGGATC CTGGGCTGCC TGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TTTCCTGGAG CTGTAGGACC TGCAGGACCA 
CCAGGATTAC AAGGTCCTCC AGGGCCTCCT GGTCCTCTTG GTCCTGATGG GAATATGGGG 
CTAGGTTTTC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCTGG CCCAGCAGGA 
CCTCCACCAT CTACTGGAGA GCTGGAATTC ATGGGATTCC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTAA GGGTTTTCCA GGCATAAGTG GCCCTCCAGG CTTCCCGGGC 
CTTGGAACTA CTGGAGAAAA GGGAGAAAAG GGAGAAAAGG GAATCCCTGG TTTGCCAGGA 
CCTAGGGGTC CCATGGGTTC AGAAGGAGTC CAAGGCCCTC CAGGGCAACA GGGCAAGAAA 
GGGACCCTGG GATTTCCTGG GCTTAATGGA TTCCAAGGAA TTGAGGGTCA AAAGGGTGAC 
ATTGGCCTGC CAGGCCCAGA TGTTTTCATC GATATAGATG GTGCTGTGAT CTCAGGTAAT 
CCTGGAGATC CTGGTGTACC TGGCCTCCCA GGCCTTAAAG GAGATGAAGG CATCCAAGGC 
CTACGTGGCC CTTCTGGTGT CCCTGGATTG CCAGCATTAT CAGGTGTCCC AGGAGCCCTA 
GGGCCTCAGG GATTTCCAGG GCTGAAGGGG GACCAAGGAA ACCCAGGCCG TACCACAATT 
GGAGCAGCTG GCCTCCCTGG CAGAGATGGT TTGCCAGGCC CACCAGGTCC ACCAGGCCCA 
CCTAGTCCAG AATTTGAGAC TGAAACTCTA CACAACAAAG AGTCAGGGTT CCCTGGTCTC 
CGAGGAGAAC AAGGTCCAAA AGGAAACCTA GGCCTCAAAG GAATAAAAGG AGACTCAGGT 
TTCTGTGCTT GTGACGGTGG TGTTCCCAAC ACTGGACCAC CCGGGGAACC AGGCCCACCT 
GGTCCATGGG GTCTCATAGG CCTTCCAGGC CTTAAAGGAG CCAGAGGAGA TCGAGGCTCT 
GGGGGTGCAC AGGGCCCAGC AGGGGCTCCA GGCTTAGTTG GGCCTCTGGG TCCTTCAGGA 
CCCAAAGGAA AGAAGGGGGA ACCAATTCTC AGTACAATCC AAGGAATGCC AGGAGATCGG 
GGTGATTCTG GCTCCCAGGG CTTCCGTGGT GTAATAGGAG AACCAGGCAA GGACGGAGTA 
CCAGGTTTAC CAGGTCTGCC AGGCCTTCCG GGTGATGGTG GACAGGGCTT CCCAGGTGAA 
AAGGGGTTAC CTGGACTTCC TGGTGAAAAA GGCCATCCTG GTCCACCTGG CCTCCCAGGA 
AATGGGTTAC CAGGACTTCC TGGACCCCGT GGGCTTCCTG GAGATAAAGG CAAGGATGGA 
TTACCGGGAC AACAAGGCCT TCCCGGATCT AAGGGAATCA CCCTGCCCTG TATTATTCCT 
GGGTCATACG GTCCATCAGG ATTTCCAGGC ACTCCCGGAT TCCCAGGCCC TAAAGGGTCT 
CGAGGCCTCC CTGGGACCCC AGGCCAGCCT GGGTCAAGTG GAAGTAAAGG AGAGCCAGGG 
AGTCCAGGAT TGGTTCATCT TCCTGAATTA CCAGGATTTC CTGGACCTCG TGGGGAGAAG 
GGCTTGCCTG GGTTTCCTGG GCTCCCTGGA AAAGATGGCT TGCCTGGGAT GATTGGCAGT 
CCAGGCTTAC CTGGTTCCAA GGGAGCCACT GGTGACATCT TTGGTGCTGA AAATGGTGCT 
CCGGGGGAAC AAGGCCTACA AGGATTAACA GGGCACAAAG GATTTCTTGG AGACTCTGGC 
CTTCCAGGAC TCAAGGGTGT GCACGGGAAG CCTGGCTTAC TAGGCCCCAA AGGTGAGCGG 
GGCAGCCCTG GGACACCAGG ACAGGTGGGA CAGCCAGGCA CCCCAGGATC TAGTGGTCCA 
TATGGCATCA AGGGCAAATC TGGGCTCCCA GGAGCACCAG GCTTCCCAGG CATCTCAGGA 
CATCCTGGAA AGAAAGGAAC AAGAGGCAAG AAAGGTCCTC CTGGATCAAT TGTAAAGAAA 
GGGCTGCCAG GGCTAAAAGG CCTTCCTGGA AATCCAGGCC TAGTAGGACT GAAAGGAAGC 
CCAGGCTCTC CAGGGGTCGC TGGGTTGCCA GCCCTCTCTG GACCCAAGGG AGAGAAGGGG 
TCTGTTGGAT TCGTAGGTTT TCCAGGAATA CCAGGTCTGC CTGGTATTCC TGGAACAAGA 
GGATTAAAAG GAATTCCAGG ATCAACTGGA AAAATGGGAC CATCTGGACG TGCTGGTACT 
CCTGGTGAAA AGGGAGACAG AGGCAATCCG GGGCCAGTCG GAATACCTAG TCCAAGACGT 
CCAATGTCAA ACCTTTGGCT CAAAGGAGAC AAAGGCTCTC AAGGCTCAGC CGGATCCAAT 
GGATTTCCTG GGCCAAGAGG TGACAAAGGA GAGGCTGGTC GACCTGGACC ACCAGGCCTA 
CCTGGAGCTC CTGGCCTCCC AGGCATTATC AAAGGAGTTA GTGGAAAGCC AGGGCCCCCT 
GGCTTCATGG GAATCCGGGG CTTACCTGGC CTGAAGGGGT CCTCTGGGAT CACAGGTTTC 
CCAGGAATGC CAGGAGAAAG TGGTTCACAA GGTATCAGAG GGTCGCCTGG ACTCCCAGGA 
GCATCTGGTC TCCCAGGCCT GAAAGGAGAC AACGGCCAGA CAGTTGAAAT TTCCGGTAGC 
CCAGGACCCA AGGGACAGCC TGGCGAATCT GGTTTTAAAG GCACAAAAGG AAGAGATGGA 
CTAATAGGCA ATATAGGCTT CCCTGGAAAC AAAGGTGAAG ATGGAAAAGT TGGTGTTTCT 
GGAGATGTTG GCCTTCCTGG AGCTCCAGGA TTTCCAGGAG TTGCCGGCAT GAGAGGAGAA 
CCAGGACTTC CAGGTTCTTC TGGTCACCAA GGGGCAATTG GGCCTCTAGG ATCCCCCGGA 
TTAATAGGAC CCAAAGGCTT CCCTGGATTT CCTGGTTTAC ATGGACTGAA TGGGCTTCCG 
GGCACCAAGG GTACCCATGG CACTCCAGGA CCTAGTATCA CCGGTGTGCC TGGGCCTGCT 
GGTCTCCCTG GACCCAAAGG AGAAAAAGGA TATCCAGGAA TTGGCATCGG AGCTCCAGGG 
AAGCCGGGCC TGAGAGGGCA AAAAGGTGAT CGAGGTTTCC CAGGTCTCCA GGGCCCTGCT 
GGTCTCCCCG GTGCCCCAGG CATCTCCTTG CCCTCACTCA TAGCAGGACA GCCTGGTGAC 
CCCGGGCGAC CAGGCCTAGA TGGAGAACGA GGCCGCCCAG GCCCCGCTGG ACCCCCAGGT 
CCCCCTGGGC CATCCTCGAA TCAAGGCGAC ACCGGAGACC CTGGCTTCCC TGGAATTCCT 
GGACCTAAAG GGCCTAAGGG AGACCAAGGA ATTCCAGGTT TTTCTGGCCT CCCTGGAGAG 
CTAGGACTGA AAGGCATGAG AGGTGAGCCT GGCTTCATGG GGACTCCAGG CAAGGTTGGG 
CCACCTGGAG ACCCAGGATT TCCCGGAATG AAGGGGAAGG CAGGGCCAAG AGGCTCTTCT 
GGCCTCCAAG GTGATCCTGG ACAAACACCA ACTGCAGAAG CTGTCCAGGT TCCTCCTGGA 
CCCTTGGGTC TACCAGGGAT CGATGGCATC CCTGGCCTCA CTGGGGACCC TGGGGCTCAA 
GGCCCTGTAG GCCTACAAGG CTCCAAAGGT TTACCTGGCA TCCCCGGTAA AGATGGCCCC 
AGTGGGCTCC CAGGCCCACC TGGGGCTCTT GGTGATCCTG GTCTGCCTGG ACTGCAAGGC 
CCTCCAGGAT TTGAAGGAGC TCCAGGGCAG CAAGGCCCCT TCGGGATGCC TGGAATGCCT 
GGCCAGAGCA TGAGAGTGGG CTACACGTTG GTAAAGCACA GCCAGTCGGA ACAGGTGCCC 
CCGTGTCCCA TCGGGATGAG CCAGCTGTGG GTGGGGTACA GCTTACTGTT TGTGGAGGGG 
CAAGAGAAAG CCCACAACCA GGACCTGGGC TTTGCTGGCT CCTGTCTGCC CCGCTTCAGC 
ACCATGCCCT TCATCTACTG CAACATCAAC GAGGTGTGCC ACTATGCCAG GCGCAATGAT 
AAATCTTACT GGCTCTCCAC TACCGCCCCT ATCCCCATGA TGCCCGTCAG CCAGACCCAG 
ATTCCCCAGT ACATCAGCCG CTGCTCTGTG TGTGAGGCAC CCTCGCAAGC CATTGCTGTG 
CACAGCCAGG ACATCACCAT CCCGCAGTGC CCCCTGGGCT GGCGCAGCCT CTGGATTGGG 
TACTCTTTCC TCATGCACAC TGCCGCTGGT GCCGAGGGTG GAGGCCAGTC CCTGGTCTCA 
CCTGGCTCCT GCCTAGAGGA CTTTCGGGCC ACTCCTTTCA TCGAATGCAG TGGTGCCCGA 
GGCACCTGCC ACTACTTTGC AAACAAGTAC AGTTTCTGGT TGACCACAGT GGAGGAGAGG 
CAGCAGTTTG GGGAGTTGCC TGTGTCTGAA ACGCTGAAAG CTGGGCAGCT CCACACTCGA 
GTCAGTCGCT GCCAGGTGTG TATGAAAAGC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( XM_006724617.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724680.1 Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant X1, mRNA.

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CloneID	OHu54120
Clone ID Related Accession (Same CDS sequence)	XM_006724617.3
Accession Version	XM_006724617.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006724617.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTATAA ACAAGTTGTG GCTGCTCCTG GTTACGTTGT GCCTGACCGA GGAACTGGCA 
GCAGCGGGAG AGAAGTCTTA TGGAAAGCCA TGTGGGGGCC AGGACTGCAG TGGGAGCTGT 
CAGTGTTTTC CTGAGAAAGG AGCGAGAGGA CGACCTGGAC CAATTGGAAT TCAAGGCCCA 
ACAGGTCCTC AAGGATTCAC TGGCTCTACT GGTTTATCGG GATTGAAAGG AGAAAGGGGT 
TTCCCAGGCC TTCTGGGACC TTATGGACCA AAAGGAGATA AGGGTCCCAT GGGAGTTCCT 
GGCTTTCTTG GCATCAATGG GATTCCGGGC CACCCTGGAC AACCAGGCCC CAGAGGCCCA 
CCTGGTCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATCCTGGGC TTCTCGGACC ACCCGGGCTT CCTGGTCAGA AAGGATCAAA AGGTGACCCT 
GTCCTTGCTC CAGGTAGTTT CAAAGGAATG AAGGGGGATC CTGGGCTGCC TGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TTTCCTGGAG CTGTAGGACC TGCAGGACCA 
CCAGGATTAC AAGGTCCTCC AGGGCCTCCT GGTCCTCTTG GTCCTGATGG GAATATGGGG 
CTAGGTTTTC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCTGG CCCAGCAGGA 
CCTCCACCAT CTACTGGAGA GCTGGAATTC ATGGGATTCC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTAA GGGTTTTCCA GGCATAAGTG GCCCTCCAGG CTTCCCGGGC 
CTTGGAACTA CTGGAGAAAA GGGAGAAAAG GGAGAAAAGG GAATCCCTGG TTTGCCAGGA 
CCTAGGGGTC CCATGGGTTC AGAAGGAGTC CAAGGCCCTC CAGGGCAACA GGGCAAGAAA 
GGGACCCTGG GATTTCCTGG GCTTAATGGA TTCCAAGGAA TTGAGGGTCA AAAGGGTGAC 
ATTGGCCTGC CAGGCCCAGA TGTTTTCATC GATATAGATG GTGCTGTGAT CTCAGGTAAT 
CCTGGAGATC CTGGTGTACC TGGCCTCCCA GGCCTTAAAG GAGATGAAGG CATCCAAGGC 
CTACGTGGCC CTTCTGGTGT CCCTGGATTG CCAGCATTAT CAGGTGTCCC AGGAGCCCTA 
GGGCCTCAGG GATTTCCAGG GCTGAAGGGG GACCAAGGAA ACCCAGGCCG TACCACAATT 
GGAGCAGCTG GCCTCCCTGG CAGAGATGGT TTGCCAGGCC CACCAGGTCC ACCAGGCCCA 
CCTAGTCCAG AATTTGAGAC TGAAACTCTA CACAACAAAG AGTCAGGGTT CCCTGGTCTC 
CGAGGAGAAC AAGGTCCAAA AGGAAACCTA GGCCTCAAAG GAATAAAAGG AGACTCAGGT 
TTCTGTGCTT GTGACGGTGG TGTTCCCAAC ACTGGACCAC CCGGGGAACC AGGCCCACCT 
GGTCCATGGG GTCTCATAGG CCTTCCAGGC CTTAAAGGAG CCAGAGGAGA TCGAGGCTCT 
GGGGGTGCAC AGGGCCCAGC AGGGGCTCCA GGCTTAGTTG GGCCTCTGGG TCCTTCAGGA 
CCCAAAGGAA AGAAGGGGGA ACCAATTCTC AGTACAATCC AAGGAATGCC AGGAGATCGG 
GGTGATTCTG GCTCCCAGGG CTTCCGTGGT GTAATAGGAG AACCAGGCAA GGACGGAGTA 
CCAGGTTTAC CAGGTCTGCC AGGCCTTCCG GGTGATGGTG GACAGGGCTT CCCAGGTGAA 
AAGGGGTTAC CTGGACTTCC TGGTGAAAAA GGCCATCCTG GTCCACCTGG CCTCCCAGGA 
AATGGGTTAC CAGGACTTCC TGGACCCCGT GGGCTTCCTG GAGATAAAGG CAAGGATGGA 
TTACCGGGAC AACAAGGCCT TCCCGGATCT AAGGGTGACT GCTGCTGTAG AGAGGTTGGT 
AAAGGAGACT TAGACACAGA GAGAGGAATC ACCCTGCCCT GTATTATTCC TGGGTCATAC 
GGTCCATCAG GATTTCCAGG CACTCCCGGA TTCCCAGGCC CTAAAGGGTC TCGAGGCCTC 
CCTGGGACCC CAGGCCAGCC TGGGTCAAGT GGAAGTAAAG GAGAGCCAGG GAGTCCAGGA 
TTGGTTCATC TTCCTGAATT ACCAGGATTT CCTGGACCTC GTGGGGAGAA GGGCTTGCCT 
GGGTTTCCTG GGCTCCCTGG AAAAGATGGC TTGCCTGGGA TGATTGGCAG TCCAGGCTTA 
CCTGGTTCCA AGGGAGCCAC TGGTGACATC TTTGGTGCTG AAAATGGTGC TCCGGGGGAA 
CAAGGCCTAC AAGGATTAAC AGGGCACAAA GGATTTCTTG GAGACTCTGG CCTTCCAGGA 
CTCAAGGGTG TGCACGGGAA GCCTGGCTTA CTAGGCCCCA AAGGTGAGCG GGGCAGCCCT 
GGGACACCAG GACAGGTGGG ACAGCCAGGC ACCCCAGGAT CTAGTGGTCC ATATGGCATC 
AAGGGCAAAT CTGGGCTCCC AGGAGCACCA GGCTTCCCAG GCATCTCAGG ACATCCTGGA 
AAGAAAGGAA CAAGAGGCAA GAAAGGTCCT CCTGGATCAA TTGTAAAGAA AGGGCTGCCA 
GGGCTAAAAG GCCTTCCTGG AAATCCAGGC CTAGTAGGAC TGAAAGGAAG CCCAGGCTCT 
CCAGGGGTCG CTGGGTTGCC AGCCCTCTCT GGACCCAAGG GAGAGAAGGG GTCTGTTGGA 
TTCGTAGGTT TTCCAGGAAT ACCAGGTCTG CCTGGTATTC CTGGAACAAG AGGATTAAAA 
GGAATTCCAG GATCAACTGG AAAAATGGGA CCATCTGGAC GTGCTGGTAC TCCTGGTGAA 
AAGGGAGACA GAGGCAATCC GGGGCCAGTC GGAATACCTA GTCCAAGACG TCCAATGTCA 
AACCTTTGGC TCAAAGGAGA CAAAGGCTCT CAAGGCTCAG CCGGATCCAA TGGATTTCCT 
GGGCCAAGAG GTGACAAAGG AGAGGCTGGT CGACCTGGAC CACCAGGCCT ACCTGGAGCT 
CCTGGCCTCC CAGGCATTAT CAAAGGAGTT AGTGGAAAGC CAGGGCCCCC TGGCTTCATG 
GGAATCCGGG GCTTACCTGG CCTGAAGGGG TCCTCTGGGA TCACAGGTTT CCCAGGAATG 
CCAGGAGAAA GTGGTTCACA AGGTATCAGA GGGTCGCCTG GACTCCCAGG AGCATCTGGT 
CTCCCAGGCC TGAAAGGAGA CAACGGCCAG ACAGTTGAAA TTTCCGGTAG CCCAGGACCC 
AAGGGACAGC CTGGCGAATC TGGTTTTAAA GGCACAAAAG GAAGAGATGG ACTAATAGGC 
AATATAGGCT TCCCTGGAAA CAAAGGTGAA GATGGAAAAG TTGGTGTTTC TGGAGATGTT 
GGCCTTCCTG GAGCTCCAGG ATTTCCAGGA GTTGCCGGCA TGAGAGGAGA ACCAGGACTT 
CCAGGTTCTT CTGGTCACCA AGGGGCAATT GGGCCTCTAG GATCCCCCGG ATTAATAGGA 
CCCAAAGGCT TCCCTGGATT TCCTGGTTTA CATGGACTGA ATGGGCTTCC GGGCACCAAG 
GGTACCCATG GCACTCCAGG ACCTAGTATC ACCGGTGTGC CTGGGCCTGC TGGTCTCCCT 
GGACCCAAAG GAGAAAAAGG ATATCCAGGA ATTGGCATCG GAGCTCCAGG GAAGCCGGGC 
CTGAGAGGGC AAAAAGGTGA TCGAGGTTTC CCAGGTCTCC AGGGCCCTGC TGGTCTCCCC 
GGTGCCCCAG GCATCTCCTT GCCCTCACTC ATAGCAGGAC AGCCTGGTGA CCCCGGGCGA 
CCAGGCCTAG ATGGAGAACG AGGCCGCCCA GGCCCCGCTG GACCCCCAGG TCCCCCTGGG 
CCATCCTCGA ATCAAGGCGA CACCGGAGAC CCTGGCTTCC CTGGAATTCC TGGACCTAAA 
GGGCCTAAGG GAGACCAAGG AATTCCAGGT TTTTCTGGCC TCCCTGGAGA GCTAGGACTG 
AAAGGCATGA GAGGTGAGCC TGGCTTCATG GGGACTCCAG GCAAGGTTGG GCCACCTGGA 
GACCCAGGAT TTCCCGGAAT GAAGGGGAAG GCAGGGCCAA GAGGCTCTTC TGGCCTCCAA 
GGTGATCCTG GACAAACACC AACTGCAGAA GCTGTCCAGG TTCCTCCTGG ACCCTTGGGT 
CTACCAGGGA TCGATGGCAT CCCTGGCCTC ACTGGGGACC CTGGGGCTCA AGGCCCTGTA 
GGCCTACAAG GCTCCAAAGG TTTACCTGGC ATCCCCGGTA AAGATGGCCC CAGTGGGCTC 
CCAGGCCCAC CTGGGGCTCT TGGTGATCCT GGTCTGCCTG GACTGCAAGG CCCTCCAGGA 
TTTGAAGGAG CTCCAGGGCA GCAAGGCCCC TTCGGGATGC CTGGAATGCC TGGCCAGAGC 
ATGAGAGTGG GCTACACGTT GGTAAAGCAC AGCCAGTCGG AACAGGTGCC CCCGTGTCCC 
ATCGGGATGA GCCAGCTGTG GGTGGGGTAC AGCTTACTGT TTGTGGAGGG GCAAGAGAAA 
GCCCACAACC AGGACCTGGG CTTTGCTGGC TCCTGTCTGC CCCGCTTCAG CACCATGCCC 
TTCATCTACT GCAACATCAA CGAGGTGTGC CACTATGCCA GGCGCAATGA TAAATCTTAC 
TGGCTCTCCA CTACCGCCCC TATCCCCATG ATGCCCGTCA GCCAGACCCA GATTCCCCAG 
TACATCAGCC GCTGCTCTGT GTGTGAGGCA CCCTCGCAAG CCATTGCTGT GCACAGCCAG 
GACATCACCA TCCCGCAGTG CCCCCTGGGC TGGCGCAGCC TCTGGATTGG GTACTCTTTC 
CTCATGCACA CTGCCGCTGG TGCCGAGGGT GGAGGCCAGT CCCTGGTCTC ACCTGGCTCC 
TGCCTAGAGG ACTTTCGGGC CACTCCTTTC ATCGAATGCA GTGGTGCCCG AGGCACCTGC 
CACTACTTTG CAAACAAGTA CAGTTTCTGG TTGACCACAG TGGAGGAGAG GCAGCAGTTT 
GGGGAGTTGC CTGTGTCTGA AACGCTGAAA GCTGGGCAGC TCCACACTCG AGTCAGTCGC 
TGCCAGGTGT GTATGAAAAG CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724680.1
CDS	121..5247
Translation	MLINKLWLLLVTLCLTEELAAAGEKSYGKPCGGQDCSGSCQCFPEKGARGRPGPIGIQGPTGPQGFTGSTGLSGLKGERGFPGLLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYPGLLGPPGLPGQKGSKGDPVLAPGSFKGMKGDPGLPGLDGITGPQGAPGFPGAVGPAGPPGLQGPPGPPGPLGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPKGFPGISGPPGFPGLGTTGEKGEKGEKGIPGLPGPRGPMGSEGVQGPPGQQGKKGTLGFPGLNGFQGIEGQKGDIGLPGPDVFIDIDGAVISGNPGDPGVPGLPGLKGDEGIQGLRGPSGVPGLPALSGVPGALGPQGFPGLKGDQGNPGRTTIGAAGLPGRDGLPGPPGPPGPPSPEFETETLHNKESGFPGLRGEQGPKGNLGLKGIKGDSGFCACDGGVPNTGPPGEPGPPGPWGLIGLPGLKGARGDRGSGGAQGPAGAPGLVGPLGPSGPKGKKGEPILSTIQGMPGDRGDSGSQGFRGVIGEPGKDGVPGLPGLPGLPGDGGQGFPGEKGLPGLPGEKGHPGPPGLPGNGLPGLPGPRGLPGDKGKDGLPGQQGLPGSKGDCCCREVGKGDLDTERGITLPCIIPGSYGPSGFPGTPGFPGPKGSRGLPGTPGQPGSSGSKGEPGSPGLVHLPELPGFPGPRGEKGLPGFPGLPGKDGLPGMIGSPGLPGSKGATGDIFGAENGAPGEQGLQGLTGHKGFLGDSGLPGLKGVHGKPGLLGPKGERGSPGTPGQVGQPGTPGSSGPYGIKGKSGLPGAPGFPGISGHPGKKGTRGKKGPPGSIVKKGLPGLKGLPGNPGLVGLKGSPGSPGVAGLPALSGPKGEKGSVGFVGFPGIPGLPGIPGTRGLKGIPGSTGKMGPSGRAGTPGEKGDRGNPGPVGIPSPRRPMSNLWLKGDKGSQGSAGSNGFPGPRGDKGEAGRPGPPGLPGAPGLPGIIKGVSGKPGPPGFMGIRGLPGLKGSSGITGFPGMPGESGSQGIRGSPGLPGASGLPGLKGDNGQTVEISGSPGPKGQPGESGFKGTKGRDGLIGNIGFPGNKGEDGKVGVSGDVGLPGAPGFPGVAGMRGEPGLPGSSGHQGAIGPLGSPGLIGPKGFPGFPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGYPGIGIGAPGKPGLRGQKGDRGFPGLQGPAGLPGAPGISLPSLIAGQPGDPGRPGLDGERGRPGPAGPPGPPGPSSNQGDTGDPGFPGIPGPKGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGFPGMKGKAGPRGSSGLQGDPGQTPTAEAVQVPPGPLGLPGIDGIPGLTGDPGAQGPVGLQGSKGLPGIPGKDGPSGLPGPPGALGDPGLPGLQGPPGFEGAPGQQGPFGMPGMPGQSMRVGYTLVKHSQSEQVPPCPIGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVSQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGELPVSETLKAGQLHTRVSRCQVCMKSL

Target ORF information:

RefSeq Version	XM_006724617.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724617.3

ORF Insert Sequence:

ATGCTTATAA ACAAGTTGTG GCTGCTCCTG GTTACGTTGT GCCTGACCGA GGAACTGGCA 
GCAGCGGGAG AGAAGTCTTA TGGAAAGCCA TGTGGGGGCC AGGACTGCAG TGGGAGCTGT 
CAGTGTTTTC CTGAGAAAGG AGCGAGAGGA CGACCTGGAC CAATTGGAAT TCAAGGCCCA 
ACAGGTCCTC AAGGATTCAC TGGCTCTACT GGTTTATCGG GATTGAAAGG AGAAAGGGGT 
TTCCCAGGCC TTCTGGGACC TTATGGACCA AAAGGAGATA AGGGTCCCAT GGGAGTTCCT 
GGCTTTCTTG GCATCAATGG GATTCCGGGC CACCCTGGAC AACCAGGCCC CAGAGGCCCA 
CCTGGTCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATCCTGGGC TTCTCGGACC ACCCGGGCTT CCTGGTCAGA AAGGATCAAA AGGTGACCCT 
GTCCTTGCTC CAGGTAGTTT CAAAGGAATG AAGGGGGATC CTGGGCTGCC TGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TTTCCTGGAG CTGTAGGACC TGCAGGACCA 
CCAGGATTAC AAGGTCCTCC AGGGCCTCCT GGTCCTCTTG GTCCTGATGG GAATATGGGG 
CTAGGTTTTC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCTGG CCCAGCAGGA 
CCTCCACCAT CTACTGGAGA GCTGGAATTC ATGGGATTCC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTAA GGGTTTTCCA GGCATAAGTG GCCCTCCAGG CTTCCCGGGC 
CTTGGAACTA CTGGAGAAAA GGGAGAAAAG GGAGAAAAGG GAATCCCTGG TTTGCCAGGA 
CCTAGGGGTC CCATGGGTTC AGAAGGAGTC CAAGGCCCTC CAGGGCAACA GGGCAAGAAA 
GGGACCCTGG GATTTCCTGG GCTTAATGGA TTCCAAGGAA TTGAGGGTCA AAAGGGTGAC 
ATTGGCCTGC CAGGCCCAGA TGTTTTCATC GATATAGATG GTGCTGTGAT CTCAGGTAAT 
CCTGGAGATC CTGGTGTACC TGGCCTCCCA GGCCTTAAAG GAGATGAAGG CATCCAAGGC 
CTACGTGGCC CTTCTGGTGT CCCTGGATTG CCAGCATTAT CAGGTGTCCC AGGAGCCCTA 
GGGCCTCAGG GATTTCCAGG GCTGAAGGGG GACCAAGGAA ACCCAGGCCG TACCACAATT 
GGAGCAGCTG GCCTCCCTGG CAGAGATGGT TTGCCAGGCC CACCAGGTCC ACCAGGCCCA 
CCTAGTCCAG AATTTGAGAC TGAAACTCTA CACAACAAAG AGTCAGGGTT CCCTGGTCTC 
CGAGGAGAAC AAGGTCCAAA AGGAAACCTA GGCCTCAAAG GAATAAAAGG AGACTCAGGT 
TTCTGTGCTT GTGACGGTGG TGTTCCCAAC ACTGGACCAC CCGGGGAACC AGGCCCACCT 
GGTCCATGGG GTCTCATAGG CCTTCCAGGC CTTAAAGGAG CCAGAGGAGA TCGAGGCTCT 
GGGGGTGCAC AGGGCCCAGC AGGGGCTCCA GGCTTAGTTG GGCCTCTGGG TCCTTCAGGA 
CCCAAAGGAA AGAAGGGGGA ACCAATTCTC AGTACAATCC AAGGAATGCC AGGAGATCGG 
GGTGATTCTG GCTCCCAGGG CTTCCGTGGT GTAATAGGAG AACCAGGCAA GGACGGAGTA 
CCAGGTTTAC CAGGTCTGCC AGGCCTTCCG GGTGATGGTG GACAGGGCTT CCCAGGTGAA 
AAGGGGTTAC CTGGACTTCC TGGTGAAAAA GGCCATCCTG GTCCACCTGG CCTCCCAGGA 
AATGGGTTAC CAGGACTTCC TGGACCCCGT GGGCTTCCTG GAGATAAAGG CAAGGATGGA 
TTACCGGGAC AACAAGGCCT TCCCGGATCT AAGGGTGACT GCTGCTGTAG AGAGGTTGGT 
AAAGGAGACT TAGACACAGA GAGAGGAATC ACCCTGCCCT GTATTATTCC TGGGTCATAC 
GGTCCATCAG GATTTCCAGG CACTCCCGGA TTCCCAGGCC CTAAAGGGTC TCGAGGCCTC 
CCTGGGACCC CAGGCCAGCC TGGGTCAAGT GGAAGTAAAG GAGAGCCAGG GAGTCCAGGA 
TTGGTTCATC TTCCTGAATT ACCAGGATTT CCTGGACCTC GTGGGGAGAA GGGCTTGCCT 
GGGTTTCCTG GGCTCCCTGG AAAAGATGGC TTGCCTGGGA TGATTGGCAG TCCAGGCTTA 
CCTGGTTCCA AGGGAGCCAC TGGTGACATC TTTGGTGCTG AAAATGGTGC TCCGGGGGAA 
CAAGGCCTAC AAGGATTAAC AGGGCACAAA GGATTTCTTG GAGACTCTGG CCTTCCAGGA 
CTCAAGGGTG TGCACGGGAA GCCTGGCTTA CTAGGCCCCA AAGGTGAGCG GGGCAGCCCT 
GGGACACCAG GACAGGTGGG ACAGCCAGGC ACCCCAGGAT CTAGTGGTCC ATATGGCATC 
AAGGGCAAAT CTGGGCTCCC AGGAGCACCA GGCTTCCCAG GCATCTCAGG ACATCCTGGA 
AAGAAAGGAA CAAGAGGCAA GAAAGGTCCT CCTGGATCAA TTGTAAAGAA AGGGCTGCCA 
GGGCTAAAAG GCCTTCCTGG AAATCCAGGC CTAGTAGGAC TGAAAGGAAG CCCAGGCTCT 
CCAGGGGTCG CTGGGTTGCC AGCCCTCTCT GGACCCAAGG GAGAGAAGGG GTCTGTTGGA 
TTCGTAGGTT TTCCAGGAAT ACCAGGTCTG CCTGGTATTC CTGGAACAAG AGGATTAAAA 
GGAATTCCAG GATCAACTGG AAAAATGGGA CCATCTGGAC GTGCTGGTAC TCCTGGTGAA 
AAGGGAGACA GAGGCAATCC GGGGCCAGTC GGAATACCTA GTCCAAGACG TCCAATGTCA 
AACCTTTGGC TCAAAGGAGA CAAAGGCTCT CAAGGCTCAG CCGGATCCAA TGGATTTCCT 
GGGCCAAGAG GTGACAAAGG AGAGGCTGGT CGACCTGGAC CACCAGGCCT ACCTGGAGCT 
CCTGGCCTCC CAGGCATTAT CAAAGGAGTT AGTGGAAAGC CAGGGCCCCC TGGCTTCATG 
GGAATCCGGG GCTTACCTGG CCTGAAGGGG TCCTCTGGGA TCACAGGTTT CCCAGGAATG 
CCAGGAGAAA GTGGTTCACA AGGTATCAGA GGGTCGCCTG GACTCCCAGG AGCATCTGGT 
CTCCCAGGCC TGAAAGGAGA CAACGGCCAG ACAGTTGAAA TTTCCGGTAG CCCAGGACCC 
AAGGGACAGC CTGGCGAATC TGGTTTTAAA GGCACAAAAG GAAGAGATGG ACTAATAGGC 
AATATAGGCT TCCCTGGAAA CAAAGGTGAA GATGGAAAAG TTGGTGTTTC TGGAGATGTT 
GGCCTTCCTG GAGCTCCAGG ATTTCCAGGA GTTGCCGGCA TGAGAGGAGA ACCAGGACTT 
CCAGGTTCTT CTGGTCACCA AGGGGCAATT GGGCCTCTAG GATCCCCCGG ATTAATAGGA 
CCCAAAGGCT TCCCTGGATT TCCTGGTTTA CATGGACTGA ATGGGCTTCC GGGCACCAAG 
GGTACCCATG GCACTCCAGG ACCTAGTATC ACCGGTGTGC CTGGGCCTGC TGGTCTCCCT 
GGACCCAAAG GAGAAAAAGG ATATCCAGGA ATTGGCATCG GAGCTCCAGG GAAGCCGGGC 
CTGAGAGGGC AAAAAGGTGA TCGAGGTTTC CCAGGTCTCC AGGGCCCTGC TGGTCTCCCC 
GGTGCCCCAG GCATCTCCTT GCCCTCACTC ATAGCAGGAC AGCCTGGTGA CCCCGGGCGA 
CCAGGCCTAG ATGGAGAACG AGGCCGCCCA GGCCCCGCTG GACCCCCAGG TCCCCCTGGG 
CCATCCTCGA ATCAAGGCGA CACCGGAGAC CCTGGCTTCC CTGGAATTCC TGGACCTAAA 
GGGCCTAAGG GAGACCAAGG AATTCCAGGT TTTTCTGGCC TCCCTGGAGA GCTAGGACTG 
AAAGGCATGA GAGGTGAGCC TGGCTTCATG GGGACTCCAG GCAAGGTTGG GCCACCTGGA 
GACCCAGGAT TTCCCGGAAT GAAGGGGAAG GCAGGGCCAA GAGGCTCTTC TGGCCTCCAA 
GGTGATCCTG GACAAACACC AACTGCAGAA GCTGTCCAGG TTCCTCCTGG ACCCTTGGGT 
CTACCAGGGA TCGATGGCAT CCCTGGCCTC ACTGGGGACC CTGGGGCTCA AGGCCCTGTA 
GGCCTACAAG GCTCCAAAGG TTTACCTGGC ATCCCCGGTA AAGATGGCCC CAGTGGGCTC 
CCAGGCCCAC CTGGGGCTCT TGGTGATCCT GGTCTGCCTG GACTGCAAGG CCCTCCAGGA 
TTTGAAGGAG CTCCAGGGCA GCAAGGCCCC TTCGGGATGC CTGGAATGCC TGGCCAGAGC 
ATGAGAGTGG GCTACACGTT GGTAAAGCAC AGCCAGTCGG AACAGGTGCC CCCGTGTCCC 
ATCGGGATGA GCCAGCTGTG GGTGGGGTAC AGCTTACTGT TTGTGGAGGG GCAAGAGAAA 
GCCCACAACC AGGACCTGGG CTTTGCTGGC TCCTGTCTGC CCCGCTTCAG CACCATGCCC 
TTCATCTACT GCAACATCAA CGAGGTGTGC CACTATGCCA GGCGCAATGA TAAATCTTAC 
TGGCTCTCCA CTACCGCCCC TATCCCCATG ATGCCCGTCA GCCAGACCCA GATTCCCCAG 
TACATCAGCC GCTGCTCTGT GTGTGAGGCA CCCTCGCAAG CCATTGCTGT GCACAGCCAG 
GACATCACCA TCCCGCAGTG CCCCCTGGGC TGGCGCAGCC TCTGGATTGG GTACTCTTTC 
CTCATGCACA CTGCCGCTGG TGCCGAGGGT GGAGGCCAGT CCCTGGTCTC ACCTGGCTCC 
TGCCTAGAGG ACTTTCGGGC CACTCCTTTC ATCGAATGCA GTGGTGCCCG AGGCACCTGC 
CACTACTTTG CAAACAAGTA CAGTTTCTGG TTGACCACAG TGGAGGAGAG GCAGCAGTTT 
GGGGAGTTGC CTGTGTCTGA AACGCTGAAA GCTGGGCAGC TCCACACTCG AGTCAGTCGC 
TGCCAGGTGT GTATGAAAAG CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( XM_011530852.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529154.1 Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant X2, mRNA.

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CloneID	OHu56982
Clone ID Related Accession (Same CDS sequence)	XM_011530852.2
Accession Version	XM_011530852.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTATAA ACAAGTTGTG GCTGCTCCTG GTTACGTTGT GCCTGACCGA GGAACTGGCA 
GCAGCGGGAG AGAAGTCTTA TGGAAAGCCA TGTGGGGGCC AGGACTGCAG TGGGAGCTGT 
CAGTGTTTTC CTGAGAAAGG AGCGAGAGGA CGACCTGGAC CAATTGGAAT TCAAGGCCCA 
ACAGGTCCTC AAGGATTCAC TGGCTCTACT GGTTTATCGG GATTGAAAGG AGAAAGGGGT 
TTCCCAGGCC TTCTGGGACC TTATGGACCA AAAGGAGATA AGGGTCCCAT GGGAGTTCCT 
GGCTTTCTTG GCATCAATGG GATTCCGGGC CACCCTGGAC AACCAGGCCC CAGAGGCCCA 
CCTGGTCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATCCTGGGC TTCTCGGACC ACCCGGGCTT CCTGGTCAGA AAGGATCAAA AGGTGACCCT 
GTCCTTGCTC CAGGTAGTTT CAAAGGAATG AAGGGGGATC CTGGGCTGCC TGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TTTCCTGGAG CTGTAGGACC TGCAGGACCA 
CCAGGATTAC AAGGTCCTCC AGGGCCTCCT GGTCCTCTTG GTCCTGATGG GAATATGGGG 
CTAGGTTTTC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCTGG CCCAGCAGGA 
CCTCCACCAT CTACTGGAGA GCTGGAATTC ATGGGATTCC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTAA GGGTTTTCCA GGCATAAGTG GCCCTCCAGG CTTCCCGGGC 
CTTGGAACTA CTGGAGAAAA GGGAGAAAAG GGAGAAAAGG GAATCCCTGG TTTGCCAGGA 
CCTAGGGGTC CCATGGGTTC AGAAGGAGTC CAAGGCCCTC CAGGGCAACA GGGCAAGAAA 
GGGACCCTGG GATTTCCTGG GCTTAATGGA TTCCAAGGAA TTGAGGGTCA AAAGGGTGAC 
ATTGGCCTGC CAGGCCCAGA TGTTTTCATC GATATAGATG GTGCTGTGAT CTCAGGTAAT 
CCTGGAGATC CTGGTGTACC TGGCCTCCCA GGCCTTAAAG GAGATGAAGG CATCCAAGGC 
CTACGTGGCC CTTCTGGTGT CCCTGGATTG CCAGCATTAT CAGGTGTCCC AGGAGCCCTA 
GGGCCTCAGG GATTTCCAGG GCTGAAGGGG GACCAAGGAA ACCCAGGCCG TACCACAATT 
GGAGCAGCTG GCCTCCCTGG CAGAGATGGT TTGCCAGGCC CACCAGGTCC ACCAGGCCCA 
CCTAGTCCAG AATTTGAGAC TGAAACTCTA CACAACAAAG AGTCAGGGTT CCCTGGTCTC 
CGAGGAGAAC AAGGTCCAAA AGGAAACCTA GGCCTCAAAG GAATAAAAGG AGACTCAGGT 
TTCTGTGCTT GTGACGGTGG TGTTCCCAAC ACTGGACCAC CCGGGGAACC AGGCCCACCT 
GGTCCATGGG GTCTCATAGG CCTTCCAGGC CTTAAAGGAG CCAGAGGAGA TCGAGGCTCT 
GGGGGTGCAC AGGGCCCAGC AGGGGCTCCA GGCTTAGTTG GGCCTCTGGG TCCTTCAGGA 
CCCAAAGGAA AGAAGGGGGA ACCAATTCTC AGTACAATCC AAGGAATGCC AGGAGATCGG 
GGTGATTCTG GCTCCCAGGG CTTCCGTGGT GTAATAGGAG AACCAGGCAA GGACGGAGTA 
CCAGGTTTAC CAGGTCTGCC AGGCCTTCCG GGTGATGGTG GACAGGGCTT CCCAGGTGAA 
AAGGGGTTAC CTGGACTTCC TGGTGAAAAA GGCCATCCTG GTCCACCTGG CCTCCCAGGA 
AATGGGTTAC CAGGACTTCC TGGACCCCGT GGGCTTCCTG GAGATAAAGG CAAGGATGGA 
TTACCGGGAC AACAAGGCCT TCCCGGATCT AAGGGTGACT GCTGCTGTAG AGAGGTTGGT 
AAAGGAGACT TAGACACAGA GAGAGGAATC ACCCTGCCCT GTATTATTCC TGGGTCATAC 
GGTCCATCAG GATTTCCAGG CACTCCCGGA TTCCCAGGCC CTAAAGGGTC TCGAGGCCTC 
CCTGGGACCC CAGGCCAGCC TGGGTCAAGT GGAAGTAAAG GAGAGCCAGG GAGTCCAGGA 
TTGGTTCATC TTCCTGAATT ACCAGGATTT CCTGGACCTC GTGGGGAGAA GGGCTTGCCT 
GGGTTTCCTG GGCTCCCTGG AAAAGATGGC TTGCCTGGGA TGATTGGCAG TCCAGGCTTA 
CCTGGTTCCA AGGGAGCCAC TGGTGACATC TTTGGTGCTG AAAATGGTGC TCCGGGGGAA 
CAAGGCCTAC AAGGATTAAC AGGGCACAAA GGATTTCTTG GAGACTCTGG CCTTCCAGGA 
CTCAAGGGTG TGCACGGGAA GCCTGGCTTA CTAGGCCCCA AAGGTGAGCG GGGCAGCCCT 
GGGACACCAG GACAGGTGGG ACAGCCAGGC ACCCCAGGAT CTAGTGGTCC ATATGGCATC 
AAGGGCAAAT CTGGGCTCCC AGGAGCACCA GGCTTCCCAG GCATCTCAGG ACATCCTGGA 
AAGAAAGGAA CAAGAGGCAA GAAAGGTCCT CCTGGATCAA TTGTAAAGAA AGGGCTGCCA 
GGGCTAAAAG GCCTTCCTGG AAATCCAGGC CTAGTAGGAC TGAAAGGAAG CCCAGGCTCT 
CCAGGGGTCG CTGGGTTGCC AGCCCTCTCT GGACCCAAGG GAGAGAAGGG GTCTGTTGGA 
TTCGTAGGTT TTCCAGGAAT ACCAGGTCTG CCTGGTATTC CTGGAACAAG AGGATTAAAA 
GGAATTCCAG GATCAACTGG AAAAATGGGA CCATCTGGAC GTGCTGGTAC TCCTGGTGAA 
AAGGGAGACA GAGGCAATCC GGGGCCAGTC GGAATACCTA GTCCAAGACG TCCAATGTCA 
AACCTTTGGC TCAAAGGAGA CAAAGGCTCT CAAGGCTCAG CCGGATCCAA TGGATTTCCT 
GGGCCAAGAG GTGACAAAGG AGAGGCTGGT CGACCTGGAC CACCAGGCCT ACCTGGAGCT 
CCTGGCCTCC CAGGCATTAT CAAAGGAGTT AGTGGAAAGC CAGGGCCCCC TGGCTTCATG 
GGAATCCGGG GCTTACCTGG CCTGAAGGGG TCCTCTGGGA TCACAGGTTT CCCAGGAATG 
CCAGGAGAAA GTGGTTCACA AGGTATCAGA GGGTCGCCTG GACTCCCAGG AGCATCTGGT 
CTCCCAGGCC TGAAAGGAGA CAACGGCCAG ACAGTTGAAA TTTCCGGTAG CCCAGGACCC 
AAGGGACAGC CTGGCGAATC TGGTTTTAAA GGCACAAAAG GAAGAGATGG ACTAATAGGC 
AATATAGGCT TCCCTGGAAA CAAAGGTGAA GATGGAAAAG TTGGTGTTTC TGGAGATGTT 
GGCCTTCCTG GAGCTCCAGG ATTTCCAGGA GTTGCCGGCA TGAGAGGAGA ACCAGGACTT 
CCAGGTTCTT CTGGTCACCA AGGGGCAATT GGGCCTCTAG GATCCCCCGG ATTAATAGGA 
CCCAAAGGAC CTAGTATCAC CGGTGTGCCT GGGCCTGCTG GTCTCCCTGG ACCCAAAGGA 
GAAAAAGGAT ATCCAGGAAT TGGCATCGGA GCTCCAGGGA AGCCGGGCCT GAGAGGGCAA 
AAAGGTGATC GAGGTTTCCC AGGTCTCCAG GGCCCTGCTG GTCTCCCCGG TGCCCCAGGC 
ATCTCCTTGC CCTCACTCAT AGCAGGACAG CCTGGTGACC CCGGGCGACC AGGCCTAGAT 
GGAGAACGAG GCCGCCCAGG CCCCGCTGGA CCCCCAGGTC CCCCTGGGCC ATCCTCGAAT 
CAAGGCGACA CCGGAGACCC TGGCTTCCCT GGAATTCCTG GACCTAAAGG GCCTAAGGGA 
GACCAAGGAA TTCCAGGTTT TTCTGGCCTC CCTGGAGAGC TAGGACTGAA AGGCATGAGA 
GGTGAGCCTG GCTTCATGGG GACTCCAGGC AAGGTTGGGC CACCTGGAGA CCCAGGATTT 
CCCGGAATGA AGGGGAAGGC AGGGCCAAGA GGCTCTTCTG GCCTCCAAGG TGATCCTGGA 
CAAACACCAA CTGCAGAAGC TGTCCAGGTT CCTCCTGGAC CCTTGGGTCT ACCAGGGATC 
GATGGCATCC CTGGCCTCAC TGGGGACCCT GGGGCTCAAG GCCCTGTAGG CCTACAAGGC 
TCCAAAGGTT TACCTGGCAT CCCCGGTAAA GATGGCCCCA GTGGGCTCCC AGGCCCACCT 
GGGGCTCTTG GTGATCCTGG TCTGCCTGGA CTGCAAGGCC CTCCAGGATT TGAAGGAGCT 
CCAGGGCAGC AAGGCCCCTT CGGGATGCCT GGAATGCCTG GCCAGAGCAT GAGAGTGGGC 
TACACGTTGG TAAAGCACAG CCAGTCGGAA CAGGTGCCCC CGTGTCCCAT CGGGATGAGC 
CAGCTGTGGG TGGGGTACAG CTTACTGTTT GTGGAGGGGC AAGAGAAAGC CCACAACCAG 
GACCTGGGCT TTGCTGGCTC CTGTCTGCCC CGCTTCAGCA CCATGCCCTT CATCTACTGC 
AACATCAACG AGGTGTGCCA CTATGCCAGG CGCAATGATA AATCTTACTG GCTCTCCACT 
ACCGCCCCTA TCCCCATGAT GCCCGTCAGC CAGACCCAGA TTCCCCAGTA CATCAGCCGC 
TGCTCTGTGT GTGAGGCACC CTCGCAAGCC ATTGCTGTGC ACAGCCAGGA CATCACCATC 
CCGCAGTGCC CCCTGGGCTG GCGCAGCCTC TGGATTGGGT ACTCTTTCCT CATGCACACT 
GCCGCTGGTG CCGAGGGTGG AGGCCAGTCC CTGGTCTCAC CTGGCTCCTG CCTAGAGGAC 
TTTCGGGCCA CTCCTTTCAT CGAATGCAGT GGTGCCCGAG GCACCTGCCA CTACTTTGCA 
AACAAGTACA GTTTCTGGTT GACCACAGTG GAGGAGAGGC AGCAGTTTGG GGAGTTGCCT 
GTGTCTGAAA CGCTGAAAGC TGGGCAGCTC CACACTCGAG TCAGTCGCTG CCAGGTGTGT 
ATGAAAAGCC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529154.1
CDS	121..5175
Translation	MLINKLWLLLVTLCLTEELAAAGEKSYGKPCGGQDCSGSCQCFPEKGARGRPGPIGIQGPTGPQGFTGSTGLSGLKGERGFPGLLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYPGLLGPPGLPGQKGSKGDPVLAPGSFKGMKGDPGLPGLDGITGPQGAPGFPGAVGPAGPPGLQGPPGPPGPLGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPKGFPGISGPPGFPGLGTTGEKGEKGEKGIPGLPGPRGPMGSEGVQGPPGQQGKKGTLGFPGLNGFQGIEGQKGDIGLPGPDVFIDIDGAVISGNPGDPGVPGLPGLKGDEGIQGLRGPSGVPGLPALSGVPGALGPQGFPGLKGDQGNPGRTTIGAAGLPGRDGLPGPPGPPGPPSPEFETETLHNKESGFPGLRGEQGPKGNLGLKGIKGDSGFCACDGGVPNTGPPGEPGPPGPWGLIGLPGLKGARGDRGSGGAQGPAGAPGLVGPLGPSGPKGKKGEPILSTIQGMPGDRGDSGSQGFRGVIGEPGKDGVPGLPGLPGLPGDGGQGFPGEKGLPGLPGEKGHPGPPGLPGNGLPGLPGPRGLPGDKGKDGLPGQQGLPGSKGDCCCREVGKGDLDTERGITLPCIIPGSYGPSGFPGTPGFPGPKGSRGLPGTPGQPGSSGSKGEPGSPGLVHLPELPGFPGPRGEKGLPGFPGLPGKDGLPGMIGSPGLPGSKGATGDIFGAENGAPGEQGLQGLTGHKGFLGDSGLPGLKGVHGKPGLLGPKGERGSPGTPGQVGQPGTPGSSGPYGIKGKSGLPGAPGFPGISGHPGKKGTRGKKGPPGSIVKKGLPGLKGLPGNPGLVGLKGSPGSPGVAGLPALSGPKGEKGSVGFVGFPGIPGLPGIPGTRGLKGIPGSTGKMGPSGRAGTPGEKGDRGNPGPVGIPSPRRPMSNLWLKGDKGSQGSAGSNGFPGPRGDKGEAGRPGPPGLPGAPGLPGIIKGVSGKPGPPGFMGIRGLPGLKGSSGITGFPGMPGESGSQGIRGSPGLPGASGLPGLKGDNGQTVEISGSPGPKGQPGESGFKGTKGRDGLIGNIGFPGNKGEDGKVGVSGDVGLPGAPGFPGVAGMRGEPGLPGSSGHQGAIGPLGSPGLIGPKGPSITGVPGPAGLPGPKGEKGYPGIGIGAPGKPGLRGQKGDRGFPGLQGPAGLPGAPGISLPSLIAGQPGDPGRPGLDGERGRPGPAGPPGPPGPSSNQGDTGDPGFPGIPGPKGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGFPGMKGKAGPRGSSGLQGDPGQTPTAEAVQVPPGPLGLPGIDGIPGLTGDPGAQGPVGLQGSKGLPGIPGKDGPSGLPGPPGALGDPGLPGLQGPPGFEGAPGQQGPFGMPGMPGQSMRVGYTLVKHSQSEQVPPCPIGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVSQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGELPVSETLKAGQLHTRVSRCQVCMKSL

Target ORF information:

RefSeq Version	XM_011530852.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530852.2

ORF Insert Sequence:

ATGCTTATAA ACAAGTTGTG GCTGCTCCTG GTTACGTTGT GCCTGACCGA GGAACTGGCA 
GCAGCGGGAG AGAAGTCTTA TGGAAAGCCA TGTGGGGGCC AGGACTGCAG TGGGAGCTGT 
CAGTGTTTTC CTGAGAAAGG AGCGAGAGGA CGACCTGGAC CAATTGGAAT TCAAGGCCCA 
ACAGGTCCTC AAGGATTCAC TGGCTCTACT GGTTTATCGG GATTGAAAGG AGAAAGGGGT 
TTCCCAGGCC TTCTGGGACC TTATGGACCA AAAGGAGATA AGGGTCCCAT GGGAGTTCCT 
GGCTTTCTTG GCATCAATGG GATTCCGGGC CACCCTGGAC AACCAGGCCC CAGAGGCCCA 
CCTGGTCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATCCTGGGC TTCTCGGACC ACCCGGGCTT CCTGGTCAGA AAGGATCAAA AGGTGACCCT 
GTCCTTGCTC CAGGTAGTTT CAAAGGAATG AAGGGGGATC CTGGGCTGCC TGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TTTCCTGGAG CTGTAGGACC TGCAGGACCA 
CCAGGATTAC AAGGTCCTCC AGGGCCTCCT GGTCCTCTTG GTCCTGATGG GAATATGGGG 
CTAGGTTTTC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCTGG CCCAGCAGGA 
CCTCCACCAT CTACTGGAGA GCTGGAATTC ATGGGATTCC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTAA GGGTTTTCCA GGCATAAGTG GCCCTCCAGG CTTCCCGGGC 
CTTGGAACTA CTGGAGAAAA GGGAGAAAAG GGAGAAAAGG GAATCCCTGG TTTGCCAGGA 
CCTAGGGGTC CCATGGGTTC AGAAGGAGTC CAAGGCCCTC CAGGGCAACA GGGCAAGAAA 
GGGACCCTGG GATTTCCTGG GCTTAATGGA TTCCAAGGAA TTGAGGGTCA AAAGGGTGAC 
ATTGGCCTGC CAGGCCCAGA TGTTTTCATC GATATAGATG GTGCTGTGAT CTCAGGTAAT 
CCTGGAGATC CTGGTGTACC TGGCCTCCCA GGCCTTAAAG GAGATGAAGG CATCCAAGGC 
CTACGTGGCC CTTCTGGTGT CCCTGGATTG CCAGCATTAT CAGGTGTCCC AGGAGCCCTA 
GGGCCTCAGG GATTTCCAGG GCTGAAGGGG GACCAAGGAA ACCCAGGCCG TACCACAATT 
GGAGCAGCTG GCCTCCCTGG CAGAGATGGT TTGCCAGGCC CACCAGGTCC ACCAGGCCCA 
CCTAGTCCAG AATTTGAGAC TGAAACTCTA CACAACAAAG AGTCAGGGTT CCCTGGTCTC 
CGAGGAGAAC AAGGTCCAAA AGGAAACCTA GGCCTCAAAG GAATAAAAGG AGACTCAGGT 
TTCTGTGCTT GTGACGGTGG TGTTCCCAAC ACTGGACCAC CCGGGGAACC AGGCCCACCT 
GGTCCATGGG GTCTCATAGG CCTTCCAGGC CTTAAAGGAG CCAGAGGAGA TCGAGGCTCT 
GGGGGTGCAC AGGGCCCAGC AGGGGCTCCA GGCTTAGTTG GGCCTCTGGG TCCTTCAGGA 
CCCAAAGGAA AGAAGGGGGA ACCAATTCTC AGTACAATCC AAGGAATGCC AGGAGATCGG 
GGTGATTCTG GCTCCCAGGG CTTCCGTGGT GTAATAGGAG AACCAGGCAA GGACGGAGTA 
CCAGGTTTAC CAGGTCTGCC AGGCCTTCCG GGTGATGGTG GACAGGGCTT CCCAGGTGAA 
AAGGGGTTAC CTGGACTTCC TGGTGAAAAA GGCCATCCTG GTCCACCTGG CCTCCCAGGA 
AATGGGTTAC CAGGACTTCC TGGACCCCGT GGGCTTCCTG GAGATAAAGG CAAGGATGGA 
TTACCGGGAC AACAAGGCCT TCCCGGATCT AAGGGTGACT GCTGCTGTAG AGAGGTTGGT 
AAAGGAGACT TAGACACAGA GAGAGGAATC ACCCTGCCCT GTATTATTCC TGGGTCATAC 
GGTCCATCAG GATTTCCAGG CACTCCCGGA TTCCCAGGCC CTAAAGGGTC TCGAGGCCTC 
CCTGGGACCC CAGGCCAGCC TGGGTCAAGT GGAAGTAAAG GAGAGCCAGG GAGTCCAGGA 
TTGGTTCATC TTCCTGAATT ACCAGGATTT CCTGGACCTC GTGGGGAGAA GGGCTTGCCT 
GGGTTTCCTG GGCTCCCTGG AAAAGATGGC TTGCCTGGGA TGATTGGCAG TCCAGGCTTA 
CCTGGTTCCA AGGGAGCCAC TGGTGACATC TTTGGTGCTG AAAATGGTGC TCCGGGGGAA 
CAAGGCCTAC AAGGATTAAC AGGGCACAAA GGATTTCTTG GAGACTCTGG CCTTCCAGGA 
CTCAAGGGTG TGCACGGGAA GCCTGGCTTA CTAGGCCCCA AAGGTGAGCG GGGCAGCCCT 
GGGACACCAG GACAGGTGGG ACAGCCAGGC ACCCCAGGAT CTAGTGGTCC ATATGGCATC 
AAGGGCAAAT CTGGGCTCCC AGGAGCACCA GGCTTCCCAG GCATCTCAGG ACATCCTGGA 
AAGAAAGGAA CAAGAGGCAA GAAAGGTCCT CCTGGATCAA TTGTAAAGAA AGGGCTGCCA 
GGGCTAAAAG GCCTTCCTGG AAATCCAGGC CTAGTAGGAC TGAAAGGAAG CCCAGGCTCT 
CCAGGGGTCG CTGGGTTGCC AGCCCTCTCT GGACCCAAGG GAGAGAAGGG GTCTGTTGGA 
TTCGTAGGTT TTCCAGGAAT ACCAGGTCTG CCTGGTATTC CTGGAACAAG AGGATTAAAA 
GGAATTCCAG GATCAACTGG AAAAATGGGA CCATCTGGAC GTGCTGGTAC TCCTGGTGAA 
AAGGGAGACA GAGGCAATCC GGGGCCAGTC GGAATACCTA GTCCAAGACG TCCAATGTCA 
AACCTTTGGC TCAAAGGAGA CAAAGGCTCT CAAGGCTCAG CCGGATCCAA TGGATTTCCT 
GGGCCAAGAG GTGACAAAGG AGAGGCTGGT CGACCTGGAC CACCAGGCCT ACCTGGAGCT 
CCTGGCCTCC CAGGCATTAT CAAAGGAGTT AGTGGAAAGC CAGGGCCCCC TGGCTTCATG 
GGAATCCGGG GCTTACCTGG CCTGAAGGGG TCCTCTGGGA TCACAGGTTT CCCAGGAATG 
CCAGGAGAAA GTGGTTCACA AGGTATCAGA GGGTCGCCTG GACTCCCAGG AGCATCTGGT 
CTCCCAGGCC TGAAAGGAGA CAACGGCCAG ACAGTTGAAA TTTCCGGTAG CCCAGGACCC 
AAGGGACAGC CTGGCGAATC TGGTTTTAAA GGCACAAAAG GAAGAGATGG ACTAATAGGC 
AATATAGGCT TCCCTGGAAA CAAAGGTGAA GATGGAAAAG TTGGTGTTTC TGGAGATGTT 
GGCCTTCCTG GAGCTCCAGG ATTTCCAGGA GTTGCCGGCA TGAGAGGAGA ACCAGGACTT 
CCAGGTTCTT CTGGTCACCA AGGGGCAATT GGGCCTCTAG GATCCCCCGG ATTAATAGGA 
CCCAAAGGAC CTAGTATCAC CGGTGTGCCT GGGCCTGCTG GTCTCCCTGG ACCCAAAGGA 
GAAAAAGGAT ATCCAGGAAT TGGCATCGGA GCTCCAGGGA AGCCGGGCCT GAGAGGGCAA 
AAAGGTGATC GAGGTTTCCC AGGTCTCCAG GGCCCTGCTG GTCTCCCCGG TGCCCCAGGC 
ATCTCCTTGC CCTCACTCAT AGCAGGACAG CCTGGTGACC CCGGGCGACC AGGCCTAGAT 
GGAGAACGAG GCCGCCCAGG CCCCGCTGGA CCCCCAGGTC CCCCTGGGCC ATCCTCGAAT 
CAAGGCGACA CCGGAGACCC TGGCTTCCCT GGAATTCCTG GACCTAAAGG GCCTAAGGGA 
GACCAAGGAA TTCCAGGTTT TTCTGGCCTC CCTGGAGAGC TAGGACTGAA AGGCATGAGA 
GGTGAGCCTG GCTTCATGGG GACTCCAGGC AAGGTTGGGC CACCTGGAGA CCCAGGATTT 
CCCGGAATGA AGGGGAAGGC AGGGCCAAGA GGCTCTTCTG GCCTCCAAGG TGATCCTGGA 
CAAACACCAA CTGCAGAAGC TGTCCAGGTT CCTCCTGGAC CCTTGGGTCT ACCAGGGATC 
GATGGCATCC CTGGCCTCAC TGGGGACCCT GGGGCTCAAG GCCCTGTAGG CCTACAAGGC 
TCCAAAGGTT TACCTGGCAT CCCCGGTAAA GATGGCCCCA GTGGGCTCCC AGGCCCACCT 
GGGGCTCTTG GTGATCCTGG TCTGCCTGGA CTGCAAGGCC CTCCAGGATT TGAAGGAGCT 
CCAGGGCAGC AAGGCCCCTT CGGGATGCCT GGAATGCCTG GCCAGAGCAT GAGAGTGGGC 
TACACGTTGG TAAAGCACAG CCAGTCGGAA CAGGTGCCCC CGTGTCCCAT CGGGATGAGC 
CAGCTGTGGG TGGGGTACAG CTTACTGTTT GTGGAGGGGC AAGAGAAAGC CCACAACCAG 
GACCTGGGCT TTGCTGGCTC CTGTCTGCCC CGCTTCAGCA CCATGCCCTT CATCTACTGC 
AACATCAACG AGGTGTGCCA CTATGCCAGG CGCAATGATA AATCTTACTG GCTCTCCACT 
ACCGCCCCTA TCCCCATGAT GCCCGTCAGC CAGACCCAGA TTCCCCAGTA CATCAGCCGC 
TGCTCTGTGT GTGAGGCACC CTCGCAAGCC ATTGCTGTGC ACAGCCAGGA CATCACCATC 
CCGCAGTGCC CCCTGGGCTG GCGCAGCCTC TGGATTGGGT ACTCTTTCCT CATGCACACT 
GCCGCTGGTG CCGAGGGTGG AGGCCAGTCC CTGGTCTCAC CTGGCTCCTG CCTAGAGGAC 
TTTCGGGCCA CTCCTTTCAT CGAATGCAGT GGTGCCCGAG GCACCTGCCA CTACTTTGCA 
AACAAGTACA GTTTCTGGTT GACCACAGTG GAGGAGAGGC AGCAGTTTGG GGAGTTGCCT 
GTGTCTGAAA CGCTGAAAGC TGGGCAGCTC CACACTCGAG TCAGTCGCTG CCAGGTGTGT 
ATGAAAAGCC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( XM_011530853.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529155.1 Homo sapiens collagen type IV alpha 6 (COL4A6), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu56983
Clone ID Related Accession (Same CDS sequence)	XM_011530853.2 , XM_011530853.3
Accession Version	XM_011530853.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTA CTAGGTGCAG TGATGGAGTC CTGGGATCTG CCAACGAAGA TGAGCTCTTG 
CAGGGACGAC CTGGACCAAT TGGAATTCAA GGCCCAACAG GTCCTCAAGG ATTCACTGGC 
TCTACTGGTT TATCGGGATT GAAAGGAGAA AGGGGTTTCC CAGGCCTTCT GGGACCTTAT 
GGACCAAAAG GAGATAAGGG TCCCATGGGA GTTCCTGGCT TTCTTGGCAT CAATGGGATT 
CCGGGCCACC CTGGACAACC AGGCCCCAGA GGCCCACCTG GTCTGGATGG CTGTAATGGA 
ACTCAAGGAG CTGTTGGATT TCCAGGCCCT GATGGCTATC CTGGGCTTCT CGGACCACCC 
GGGCTTCCTG GTCAGAAAGG ATCAAAAGGT GACCCTGTCC TTGCTCCAGG TAGTTTCAAA 
GGAATGAAGG GGGATCCTGG GCTGCCTGGA CTGGATGGAA TCACTGGCCC ACAAGGAGCA 
CCCGGATTTC CTGGAGCTGT AGGACCTGCA GGACCACCAG GATTACAAGG TCCTCCAGGG 
CCTCCTGGTC CTCTTGGTCC TGATGGGAAT ATGGGGCTAG GTTTTCAAGG AGAGAAAGGA 
GTCAAGGGGG ATGTTGGCCT CCCTGGCCCA GCAGGACCTC CACCATCTAC TGGAGAGCTG 
GAATTCATGG GATTCCCCAA AGGGAAGAAA GGATCCAAGG GTGAACCAGG GCCTAAGGGT 
TTTCCAGGCA TAAGTGGCCC TCCAGGCTTC CCGGGCCTTG GAACTACTGG AGAAAAGGGA 
GAAAAGGGAG AAAAGGGAAT CCCTGGTTTG CCAGGACCTA GGGGTCCCAT GGGTTCAGAA 
GGAGTCCAAG GCCCTCCAGG GCAACAGGGC AAGAAAGGGA CCCTGGGATT TCCTGGGCTT 
AATGGATTCC AAGGAATTGA GGGTCAAAAG GGTGACATTG GCCTGCCAGG CCCAGATGTT 
TTCATCGATA TAGATGGTGC TGTGATCTCA GGTAATCCTG GAGATCCTGG TGTACCTGGC 
CTCCCAGGCC TTAAAGGAGA TGAAGGCATC CAAGGCCTAC GTGGCCCTTC TGGTGTCCCT 
GGATTGCCAG CATTATCAGG TGTCCCAGGA GCCCTAGGGC CTCAGGGATT TCCAGGGCTG 
AAGGGGGACC AAGGAAACCC AGGCCGTACC ACAATTGGAG CAGCTGGCCT CCCTGGCAGA 
GATGGTTTGC CAGGCCCACC AGGTCCACCA GGCCCACCTA GTCCAGAATT TGAGACTGAA 
ACTCTACACA ACAAAGAGTC AGGGTTCCCT GGTCTCCGAG GAGAACAAGG TCCAAAAGGA 
AACCTAGGCC TCAAAGGAAT AAAAGGAGAC TCAGGTTTCT GTGCTTGTGA CGGTGGTGTT 
CCCAACACTG GACCACCCGG GGAACCAGGC CCACCTGGTC CATGGGGTCT CATAGGCCTT 
CCAGGCCTTA AAGGAGCCAG AGGAGATCGA GGCTCTGGGG GTGCACAGGG CCCAGCAGGG 
GCTCCAGGCT TAGTTGGGCC TCTGGGTCCT TCAGGACCCA AAGGAAAGAA GGGGGAACCA 
ATTCTCAGTA CAATCCAAGG AATGCCAGGA GATCGGGGTG ATTCTGGCTC CCAGGGCTTC 
CGTGGTGTAA TAGGAGAACC AGGCAAGGAC GGAGTACCAG GTTTACCAGG TCTGCCAGGC 
CTTCCGGGTG ATGGTGGACA GGGCTTCCCA GGTGAAAAGG GGTTACCTGG ACTTCCTGGT 
GAAAAAGGCC ATCCTGGTCC ACCTGGCCTC CCAGGAAATG GGTTACCAGG ACTTCCTGGA 
CCCCGTGGGC TTCCTGGAGA TAAAGGCAAG GATGGATTAC CGGGACAACA AGGCCTTCCC 
GGATCTAAGG GTGACTGCTG CTGTAGAGAG GTTGGTAAAG GAGACTTAGA CACAGAGAGA 
GGAATCACCC TGCCCTGTAT TATTCCTGGG TCATACGGTC CATCAGGATT TCCAGGCACT 
CCCGGATTCC CAGGCCCTAA AGGGTCTCGA GGCCTCCCTG GGACCCCAGG CCAGCCTGGG 
TCAAGTGGAA GTAAAGGAGA GCCAGGGAGT CCAGGATTGG TTCATCTTCC TGAATTACCA 
GGATTTCCTG GACCTCGTGG GGAGAAGGGC TTGCCTGGGT TTCCTGGGCT CCCTGGAAAA 
GATGGCTTGC CTGGGATGAT TGGCAGTCCA GGCTTACCTG GTTCCAAGGG AGCCACTGGT 
GACATCTTTG GTGCTGAAAA TGGTGCTCCG GGGGAACAAG GCCTACAAGG ATTAACAGGG 
CACAAAGGAT TTCTTGGAGA CTCTGGCCTT CCAGGACTCA AGGGTGTGCA CGGGAAGCCT 
GGCTTACTAG GCCCCAAAGG TGAGCGGGGC AGCCCTGGGA CACCAGGACA GGTGGGACAG 
CCAGGCACCC CAGGATCTAG TGGTCCATAT GGCATCAAGG GCAAATCTGG GCTCCCAGGA 
GCACCAGGCT TCCCAGGCAT CTCAGGACAT CCTGGAAAGA AAGGAACAAG AGGCAAGAAA 
GGTCCTCCTG GATCAATTGT AAAGAAAGGG CTGCCAGGGC TAAAAGGCCT TCCTGGAAAT 
CCAGGCCTAG TAGGACTGAA AGGAAGCCCA GGCTCTCCAG GGGTCGCTGG GTTGCCAGCC 
CTCTCTGGAC CCAAGGGAGA GAAGGGGTCT GTTGGATTCG TAGGTTTTCC AGGAATACCA 
GGTCTGCCTG GTATTCCTGG AACAAGAGGA TTAAAAGGAA TTCCAGGATC AACTGGAAAA 
ATGGGACCAT CTGGACGTGC TGGTACTCCT GGTGAAAAGG GAGACAGAGG CAATCCGGGG 
CCAGTCGGAA TACCTAGTCC AAGACGTCCA ATGTCAAACC TTTGGCTCAA AGGAGACAAA 
GGCTCTCAAG GCTCAGCCGG ATCCAATGGA TTTCCTGGGC CAAGAGGTGA CAAAGGAGAG 
GCTGGTCGAC CTGGACCACC AGGCCTACCT GGAGCTCCTG GCCTCCCAGG CATTATCAAA 
GGAGTTAGTG GAAAGCCAGG GCCCCCTGGC TTCATGGGAA TCCGGGGCTT ACCTGGCCTG 
AAGGGGTCCT CTGGGATCAC AGGTTTCCCA GGAATGCCAG GAGAAAGTGG TTCACAAGGT 
ATCAGAGGGT CGCCTGGACT CCCAGGAGCA TCTGGTCTCC CAGGCCTGAA AGGAGACAAC 
GGCCAGACAG TTGAAATTTC CGGTAGCCCA GGACCCAAGG GACAGCCTGG CGAATCTGGT 
TTTAAAGGCA CAAAAGGAAG AGATGGACTA ATAGGCAATA TAGGCTTCCC TGGAAACAAA 
GGTGAAGATG GAAAAGTTGG TGTTTCTGGA GATGTTGGCC TTCCTGGAGC TCCAGGATTT 
CCAGGAGTTG CCGGCATGAG AGGAGAACCA GGACTTCCAG GTTCTTCTGG TCACCAAGGG 
GCAATTGGGC CTCTAGGATC CCCCGGATTA ATAGGACCCA AAGGCTTCCC TGGATTTCCT 
GGTTTACATG GACTGAATGG GCTTCCGGGC ACCAAGGGTA CCCATGGCAC TCCAGGACCT 
AGTATCACCG GTGTGCCTGG GCCTGCTGGT CTCCCTGGAC CCAAAGGAGA AAAAGGATAT 
CCAGGAATTG GCATCGGAGC TCCAGGGAAG CCGGGCCTGA GAGGGCAAAA AGGTGATCGA 
GGTTTCCCAG GTCTCCAGGG CCCTGCTGGT CTCCCCGGTG CCCCAGGCAT CTCCTTGCCC 
TCACTCATAG CAGGACAGCC TGGTGACCCC GGGCGACCAG GCCTAGATGG AGAACGAGGC 
CGCCCAGGCC CCGCTGGACC CCCAGGTCCC CCTGGGCCAT CCTCGAATCA AGGCGACACC 
GGAGACCCTG GCTTCCCTGG AATTCCTGGA CCTAAAGGGC CTAAGGGAGA CCAAGGAATT 
CCAGGTTTTT CTGGCCTCCC TGGAGAGCTA GGACTGAAAG GCATGAGAGG TGAGCCTGGC 
TTCATGGGGA CTCCAGGCAA GGTTGGGCCA CCTGGAGACC CAGGATTTCC CGGAATGAAG 
GGGAAGGCAG GGCCAAGAGG CTCTTCTGGC CTCCAAGGTG ATCCTGGACA AACACCAACT 
GCAGAAGCTG TCCAGGTTCC TCCTGGACCC TTGGGTCTAC CAGGGATCGA TGGCATCCCT 
GGCCTCACTG GGGACCCTGG GGCTCAAGGC CCTGTAGGCC TACAAGGCTC CAAAGGTTTA 
CCTGGCATCC CCGGTAAAGA TGGCCCCAGT GGGCTCCCAG GCCCACCTGG GGCTCTTGGT 
GATCCTGGTC TGCCTGGACT GCAAGGCCCT CCAGGATTTG AAGGAGCTCC AGGGCAGCAA 
GGCCCCTTCG GGATGCCTGG AATGCCTGGC CAGAGCATGA GAGTGGGCTA CACGTTGGTA 
AAGCACAGCC AGTCGGAACA GGTGCCCCCG TGTCCCATCG GGATGAGCCA GCTGTGGGTG 
GGGTACAGCT TACTGTTTGT GGAGGGGCAA GAGAAAGCCC ACAACCAGGA CCTGGGCTTT 
GCTGGCTCCT GTCTGCCCCG CTTCAGCACC ATGCCCTTCA TCTACTGCAA CATCAACGAG 
GTGTGCCACT ATGCCAGGCG CAATGATAAA TCTTACTGGC TCTCCACTAC CGCCCCTATC 
CCCATGATGC CCGTCAGCCA GACCCAGATT CCCCAGTACA TCAGCCGCTG CTCTGTGTGT 
GAGGCACCCT CGCAAGCCAT TGCTGTGCAC AGCCAGGACA TCACCATCCC GCAGTGCCCC 
CTGGGCTGGC GCAGCCTCTG GATTGGGTAC TCTTTCCTCA TGCACACTGC CGCTGGTGCC 
GAGGGTGGAG GCCAGTCCCT GGTCTCACCT GGCTCCTGCC TAGAGGACTT TCGGGCCACT 
CCTTTCATCG AATGCAGTGG TGCCCGAGGC ACCTGCCACT ACTTTGCAAA CAAGTACAGT 
TTCTGGTTGA CCACAGTGGA GGAGAGGCAG CAGTTTGGGG AGTTGCCTGT GTCTGAAACG 
CTGAAAGCTG GGCAGCTCCA CACTCGAGTC AGTCGCTGCC AGGTGTGTAT GAAAAGCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529155.1
CDS	921..5963
Translation	MSATRCSDGVLGSANEDELLQGRPGPIGIQGPTGPQGFTGSTGLSGLKGERGFPGLLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYPGLLGPPGLPGQKGSKGDPVLAPGSFKGMKGDPGLPGLDGITGPQGAPGFPGAVGPAGPPGLQGPPGPPGPLGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPKGFPGISGPPGFPGLGTTGEKGEKGEKGIPGLPGPRGPMGSEGVQGPPGQQGKKGTLGFPGLNGFQGIEGQKGDIGLPGPDVFIDIDGAVISGNPGDPGVPGLPGLKGDEGIQGLRGPSGVPGLPALSGVPGALGPQGFPGLKGDQGNPGRTTIGAAGLPGRDGLPGPPGPPGPPSPEFETETLHNKESGFPGLRGEQGPKGNLGLKGIKGDSGFCACDGGVPNTGPPGEPGPPGPWGLIGLPGLKGARGDRGSGGAQGPAGAPGLVGPLGPSGPKGKKGEPILSTIQGMPGDRGDSGSQGFRGVIGEPGKDGVPGLPGLPGLPGDGGQGFPGEKGLPGLPGEKGHPGPPGLPGNGLPGLPGPRGLPGDKGKDGLPGQQGLPGSKGDCCCREVGKGDLDTERGITLPCIIPGSYGPSGFPGTPGFPGPKGSRGLPGTPGQPGSSGSKGEPGSPGLVHLPELPGFPGPRGEKGLPGFPGLPGKDGLPGMIGSPGLPGSKGATGDIFGAENGAPGEQGLQGLTGHKGFLGDSGLPGLKGVHGKPGLLGPKGERGSPGTPGQVGQPGTPGSSGPYGIKGKSGLPGAPGFPGISGHPGKKGTRGKKGPPGSIVKKGLPGLKGLPGNPGLVGLKGSPGSPGVAGLPALSGPKGEKGSVGFVGFPGIPGLPGIPGTRGLKGIPGSTGKMGPSGRAGTPGEKGDRGNPGPVGIPSPRRPMSNLWLKGDKGSQGSAGSNGFPGPRGDKGEAGRPGPPGLPGAPGLPGIIKGVSGKPGPPGFMGIRGLPGLKGSSGITGFPGMPGESGSQGIRGSPGLPGASGLPGLKGDNGQTVEISGSPGPKGQPGESGFKGTKGRDGLIGNIGFPGNKGEDGKVGVSGDVGLPGAPGFPGVAGMRGEPGLPGSSGHQGAIGPLGSPGLIGPKGFPGFPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGYPGIGIGAPGKPGLRGQKGDRGFPGLQGPAGLPGAPGISLPSLIAGQPGDPGRPGLDGERGRPGPAGPPGPPGPSSNQGDTGDPGFPGIPGPKGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGFPGMKGKAGPRGSSGLQGDPGQTPTAEAVQVPPGPLGLPGIDGIPGLTGDPGAQGPVGLQGSKGLPGIPGKDGPSGLPGPPGALGDPGLPGLQGPPGFEGAPGQQGPFGMPGMPGQSMRVGYTLVKHSQSEQVPPCPIGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVSQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGELPVSETLKAGQLHTRVSRCQVCMKSL

Target ORF information:

RefSeq Version	XM_011530853.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 6 (COL4A6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530853.2

ORF Insert Sequence:

ATGTCTGCTA CTAGGTGCAG TGATGGAGTC CTGGGATCTG CCAACGAAGA TGAGCTCTTG 
CAGGGACGAC CTGGACCAAT TGGAATTCAA GGCCCAACAG GTCCTCAAGG ATTCACTGGC 
TCTACTGGTT TATCGGGATT GAAAGGAGAA AGGGGTTTCC CAGGCCTTCT GGGACCTTAT 
GGACCAAAAG GAGATAAGGG TCCCATGGGA GTTCCTGGCT TTCTTGGCAT CAATGGGATT 
CCGGGCCACC CTGGACAACC AGGCCCCAGA GGCCCACCTG GTCTGGATGG CTGTAATGGA 
ACTCAAGGAG CTGTTGGATT TCCAGGCCCT GATGGCTATC CTGGGCTTCT CGGACCACCC 
GGGCTTCCTG GTCAGAAAGG ATCAAAAGGT GACCCTGTCC TTGCTCCAGG TAGTTTCAAA 
GGAATGAAGG GGGATCCTGG GCTGCCTGGA CTGGATGGAA TCACTGGCCC ACAAGGAGCA 
CCCGGATTTC CTGGAGCTGT AGGACCTGCA GGACCACCAG GATTACAAGG TCCTCCAGGG 
CCTCCTGGTC CTCTTGGTCC TGATGGGAAT ATGGGGCTAG GTTTTCAAGG AGAGAAAGGA 
GTCAAGGGGG ATGTTGGCCT CCCTGGCCCA GCAGGACCTC CACCATCTAC TGGAGAGCTG 
GAATTCATGG GATTCCCCAA AGGGAAGAAA GGATCCAAGG GTGAACCAGG GCCTAAGGGT 
TTTCCAGGCA TAAGTGGCCC TCCAGGCTTC CCGGGCCTTG GAACTACTGG AGAAAAGGGA 
GAAAAGGGAG AAAAGGGAAT CCCTGGTTTG CCAGGACCTA GGGGTCCCAT GGGTTCAGAA 
GGAGTCCAAG GCCCTCCAGG GCAACAGGGC AAGAAAGGGA CCCTGGGATT TCCTGGGCTT 
AATGGATTCC AAGGAATTGA GGGTCAAAAG GGTGACATTG GCCTGCCAGG CCCAGATGTT 
TTCATCGATA TAGATGGTGC TGTGATCTCA GGTAATCCTG GAGATCCTGG TGTACCTGGC 
CTCCCAGGCC TTAAAGGAGA TGAAGGCATC CAAGGCCTAC GTGGCCCTTC TGGTGTCCCT 
GGATTGCCAG CATTATCAGG TGTCCCAGGA GCCCTAGGGC CTCAGGGATT TCCAGGGCTG 
AAGGGGGACC AAGGAAACCC AGGCCGTACC ACAATTGGAG CAGCTGGCCT CCCTGGCAGA 
GATGGTTTGC CAGGCCCACC AGGTCCACCA GGCCCACCTA GTCCAGAATT TGAGACTGAA 
ACTCTACACA ACAAAGAGTC AGGGTTCCCT GGTCTCCGAG GAGAACAAGG TCCAAAAGGA 
AACCTAGGCC TCAAAGGAAT AAAAGGAGAC TCAGGTTTCT GTGCTTGTGA CGGTGGTGTT 
CCCAACACTG GACCACCCGG GGAACCAGGC CCACCTGGTC CATGGGGTCT CATAGGCCTT 
CCAGGCCTTA AAGGAGCCAG AGGAGATCGA GGCTCTGGGG GTGCACAGGG CCCAGCAGGG 
GCTCCAGGCT TAGTTGGGCC TCTGGGTCCT TCAGGACCCA AAGGAAAGAA GGGGGAACCA 
ATTCTCAGTA CAATCCAAGG AATGCCAGGA GATCGGGGTG ATTCTGGCTC CCAGGGCTTC 
CGTGGTGTAA TAGGAGAACC AGGCAAGGAC GGAGTACCAG GTTTACCAGG TCTGCCAGGC 
CTTCCGGGTG ATGGTGGACA GGGCTTCCCA GGTGAAAAGG GGTTACCTGG ACTTCCTGGT 
GAAAAAGGCC ATCCTGGTCC ACCTGGCCTC CCAGGAAATG GGTTACCAGG ACTTCCTGGA 
CCCCGTGGGC TTCCTGGAGA TAAAGGCAAG GATGGATTAC CGGGACAACA AGGCCTTCCC 
GGATCTAAGG GTGACTGCTG CTGTAGAGAG GTTGGTAAAG GAGACTTAGA CACAGAGAGA 
GGAATCACCC TGCCCTGTAT TATTCCTGGG TCATACGGTC CATCAGGATT TCCAGGCACT 
CCCGGATTCC CAGGCCCTAA AGGGTCTCGA GGCCTCCCTG GGACCCCAGG CCAGCCTGGG 
TCAAGTGGAA GTAAAGGAGA GCCAGGGAGT CCAGGATTGG TTCATCTTCC TGAATTACCA 
GGATTTCCTG GACCTCGTGG GGAGAAGGGC TTGCCTGGGT TTCCTGGGCT CCCTGGAAAA 
GATGGCTTGC CTGGGATGAT TGGCAGTCCA GGCTTACCTG GTTCCAAGGG AGCCACTGGT 
GACATCTTTG GTGCTGAAAA TGGTGCTCCG GGGGAACAAG GCCTACAAGG ATTAACAGGG 
CACAAAGGAT TTCTTGGAGA CTCTGGCCTT CCAGGACTCA AGGGTGTGCA CGGGAAGCCT 
GGCTTACTAG GCCCCAAAGG TGAGCGGGGC AGCCCTGGGA CACCAGGACA GGTGGGACAG 
CCAGGCACCC CAGGATCTAG TGGTCCATAT GGCATCAAGG GCAAATCTGG GCTCCCAGGA 
GCACCAGGCT TCCCAGGCAT CTCAGGACAT CCTGGAAAGA AAGGAACAAG AGGCAAGAAA 
GGTCCTCCTG GATCAATTGT AAAGAAAGGG CTGCCAGGGC TAAAAGGCCT TCCTGGAAAT 
CCAGGCCTAG TAGGACTGAA AGGAAGCCCA GGCTCTCCAG GGGTCGCTGG GTTGCCAGCC 
CTCTCTGGAC CCAAGGGAGA GAAGGGGTCT GTTGGATTCG TAGGTTTTCC AGGAATACCA 
GGTCTGCCTG GTATTCCTGG AACAAGAGGA TTAAAAGGAA TTCCAGGATC AACTGGAAAA 
ATGGGACCAT CTGGACGTGC TGGTACTCCT GGTGAAAAGG GAGACAGAGG CAATCCGGGG 
CCAGTCGGAA TACCTAGTCC AAGACGTCCA ATGTCAAACC TTTGGCTCAA AGGAGACAAA 
GGCTCTCAAG GCTCAGCCGG ATCCAATGGA TTTCCTGGGC CAAGAGGTGA CAAAGGAGAG 
GCTGGTCGAC CTGGACCACC AGGCCTACCT GGAGCTCCTG GCCTCCCAGG CATTATCAAA 
GGAGTTAGTG GAAAGCCAGG GCCCCCTGGC TTCATGGGAA TCCGGGGCTT ACCTGGCCTG 
AAGGGGTCCT CTGGGATCAC AGGTTTCCCA GGAATGCCAG GAGAAAGTGG TTCACAAGGT 
ATCAGAGGGT CGCCTGGACT CCCAGGAGCA TCTGGTCTCC CAGGCCTGAA AGGAGACAAC 
GGCCAGACAG TTGAAATTTC CGGTAGCCCA GGACCCAAGG GACAGCCTGG CGAATCTGGT 
TTTAAAGGCA CAAAAGGAAG AGATGGACTA ATAGGCAATA TAGGCTTCCC TGGAAACAAA 
GGTGAAGATG GAAAAGTTGG TGTTTCTGGA GATGTTGGCC TTCCTGGAGC TCCAGGATTT 
CCAGGAGTTG CCGGCATGAG AGGAGAACCA GGACTTCCAG GTTCTTCTGG TCACCAAGGG 
GCAATTGGGC CTCTAGGATC CCCCGGATTA ATAGGACCCA AAGGCTTCCC TGGATTTCCT 
GGTTTACATG GACTGAATGG GCTTCCGGGC ACCAAGGGTA CCCATGGCAC TCCAGGACCT 
AGTATCACCG GTGTGCCTGG GCCTGCTGGT CTCCCTGGAC CCAAAGGAGA AAAAGGATAT 
CCAGGAATTG GCATCGGAGC TCCAGGGAAG CCGGGCCTGA GAGGGCAAAA AGGTGATCGA 
GGTTTCCCAG GTCTCCAGGG CCCTGCTGGT CTCCCCGGTG CCCCAGGCAT CTCCTTGCCC 
TCACTCATAG CAGGACAGCC TGGTGACCCC GGGCGACCAG GCCTAGATGG AGAACGAGGC 
CGCCCAGGCC CCGCTGGACC CCCAGGTCCC CCTGGGCCAT CCTCGAATCA AGGCGACACC 
GGAGACCCTG GCTTCCCTGG AATTCCTGGA CCTAAAGGGC CTAAGGGAGA CCAAGGAATT 
CCAGGTTTTT CTGGCCTCCC TGGAGAGCTA GGACTGAAAG GCATGAGAGG TGAGCCTGGC 
TTCATGGGGA CTCCAGGCAA GGTTGGGCCA CCTGGAGACC CAGGATTTCC CGGAATGAAG 
GGGAAGGCAG GGCCAAGAGG CTCTTCTGGC CTCCAAGGTG ATCCTGGACA AACACCAACT 
GCAGAAGCTG TCCAGGTTCC TCCTGGACCC TTGGGTCTAC CAGGGATCGA TGGCATCCCT 
GGCCTCACTG GGGACCCTGG GGCTCAAGGC CCTGTAGGCC TACAAGGCTC CAAAGGTTTA 
CCTGGCATCC CCGGTAAAGA TGGCCCCAGT GGGCTCCCAG GCCCACCTGG GGCTCTTGGT 
GATCCTGGTC TGCCTGGACT GCAAGGCCCT CCAGGATTTG AAGGAGCTCC AGGGCAGCAA 
GGCCCCTTCG GGATGCCTGG AATGCCTGGC CAGAGCATGA GAGTGGGCTA CACGTTGGTA 
AAGCACAGCC AGTCGGAACA GGTGCCCCCG TGTCCCATCG GGATGAGCCA GCTGTGGGTG 
GGGTACAGCT TACTGTTTGT GGAGGGGCAA GAGAAAGCCC ACAACCAGGA CCTGGGCTTT 
GCTGGCTCCT GTCTGCCCCG CTTCAGCACC ATGCCCTTCA TCTACTGCAA CATCAACGAG 
GTGTGCCACT ATGCCAGGCG CAATGATAAA TCTTACTGGC TCTCCACTAC CGCCCCTATC 
CCCATGATGC CCGTCAGCCA GACCCAGATT CCCCAGTACA TCAGCCGCTG CTCTGTGTGT 
GAGGCACCCT CGCAAGCCAT TGCTGTGCAC AGCCAGGACA TCACCATCCC GCAGTGCCCC 
CTGGGCTGGC GCAGCCTCTG GATTGGGTAC TCTTTCCTCA TGCACACTGC CGCTGGTGCC 
GAGGGTGGAG GCCAGTCCCT GGTCTCACCT GGCTCCTGCC TAGAGGACTT TCGGGCCACT 
CCTTTCATCG AATGCAGTGG TGCCCGAGGC ACCTGCCACT ACTTTGCAAA CAAGTACAGT 
TTCTGGTTGA CCACAGTGGA GGAGAGGCAG CAGTTTGGGG AGTTGCCTGT GTCTGAAACG 
CTGAAAGCTG GGCAGCTCCA CACTCGAGTC AGTCGCTGCC AGGTGTGTAT GAAAAGCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( XM_011530853.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529155.1 Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant X3, mRNA.

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CloneID	OHu56983
Clone ID Related Accession (Same CDS sequence)	XM_011530853.2 , XM_011530853.3
Accession Version	XM_011530853.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011530853.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTA CTAGGTGCAG TGATGGAGTC CTGGGATCTG CCAACGAAGA TGAGCTCTTG 
CAGGGACGAC CTGGACCAAT TGGAATTCAA GGCCCAACAG GTCCTCAAGG ATTCACTGGC 
TCTACTGGTT TATCGGGATT GAAAGGAGAA AGGGGTTTCC CAGGCCTTCT GGGACCTTAT 
GGACCAAAAG GAGATAAGGG TCCCATGGGA GTTCCTGGCT TTCTTGGCAT CAATGGGATT 
CCGGGCCACC CTGGACAACC AGGCCCCAGA GGCCCACCTG GTCTGGATGG CTGTAATGGA 
ACTCAAGGAG CTGTTGGATT TCCAGGCCCT GATGGCTATC CTGGGCTTCT CGGACCACCC 
GGGCTTCCTG GTCAGAAAGG ATCAAAAGGT GACCCTGTCC TTGCTCCAGG TAGTTTCAAA 
GGAATGAAGG GGGATCCTGG GCTGCCTGGA CTGGATGGAA TCACTGGCCC ACAAGGAGCA 
CCCGGATTTC CTGGAGCTGT AGGACCTGCA GGACCACCAG GATTACAAGG TCCTCCAGGG 
CCTCCTGGTC CTCTTGGTCC TGATGGGAAT ATGGGGCTAG GTTTTCAAGG AGAGAAAGGA 
GTCAAGGGGG ATGTTGGCCT CCCTGGCCCA GCAGGACCTC CACCATCTAC TGGAGAGCTG 
GAATTCATGG GATTCCCCAA AGGGAAGAAA GGATCCAAGG GTGAACCAGG GCCTAAGGGT 
TTTCCAGGCA TAAGTGGCCC TCCAGGCTTC CCGGGCCTTG GAACTACTGG AGAAAAGGGA 
GAAAAGGGAG AAAAGGGAAT CCCTGGTTTG CCAGGACCTA GGGGTCCCAT GGGTTCAGAA 
GGAGTCCAAG GCCCTCCAGG GCAACAGGGC AAGAAAGGGA CCCTGGGATT TCCTGGGCTT 
AATGGATTCC AAGGAATTGA GGGTCAAAAG GGTGACATTG GCCTGCCAGG CCCAGATGTT 
TTCATCGATA TAGATGGTGC TGTGATCTCA GGTAATCCTG GAGATCCTGG TGTACCTGGC 
CTCCCAGGCC TTAAAGGAGA TGAAGGCATC CAAGGCCTAC GTGGCCCTTC TGGTGTCCCT 
GGATTGCCAG CATTATCAGG TGTCCCAGGA GCCCTAGGGC CTCAGGGATT TCCAGGGCTG 
AAGGGGGACC AAGGAAACCC AGGCCGTACC ACAATTGGAG CAGCTGGCCT CCCTGGCAGA 
GATGGTTTGC CAGGCCCACC AGGTCCACCA GGCCCACCTA GTCCAGAATT TGAGACTGAA 
ACTCTACACA ACAAAGAGTC AGGGTTCCCT GGTCTCCGAG GAGAACAAGG TCCAAAAGGA 
AACCTAGGCC TCAAAGGAAT AAAAGGAGAC TCAGGTTTCT GTGCTTGTGA CGGTGGTGTT 
CCCAACACTG GACCACCCGG GGAACCAGGC CCACCTGGTC CATGGGGTCT CATAGGCCTT 
CCAGGCCTTA AAGGAGCCAG AGGAGATCGA GGCTCTGGGG GTGCACAGGG CCCAGCAGGG 
GCTCCAGGCT TAGTTGGGCC TCTGGGTCCT TCAGGACCCA AAGGAAAGAA GGGGGAACCA 
ATTCTCAGTA CAATCCAAGG AATGCCAGGA GATCGGGGTG ATTCTGGCTC CCAGGGCTTC 
CGTGGTGTAA TAGGAGAACC AGGCAAGGAC GGAGTACCAG GTTTACCAGG TCTGCCAGGC 
CTTCCGGGTG ATGGTGGACA GGGCTTCCCA GGTGAAAAGG GGTTACCTGG ACTTCCTGGT 
GAAAAAGGCC ATCCTGGTCC ACCTGGCCTC CCAGGAAATG GGTTACCAGG ACTTCCTGGA 
CCCCGTGGGC TTCCTGGAGA TAAAGGCAAG GATGGATTAC CGGGACAACA AGGCCTTCCC 
GGATCTAAGG GTGACTGCTG CTGTAGAGAG GTTGGTAAAG GAGACTTAGA CACAGAGAGA 
GGAATCACCC TGCCCTGTAT TATTCCTGGG TCATACGGTC CATCAGGATT TCCAGGCACT 
CCCGGATTCC CAGGCCCTAA AGGGTCTCGA GGCCTCCCTG GGACCCCAGG CCAGCCTGGG 
TCAAGTGGAA GTAAAGGAGA GCCAGGGAGT CCAGGATTGG TTCATCTTCC TGAATTACCA 
GGATTTCCTG GACCTCGTGG GGAGAAGGGC TTGCCTGGGT TTCCTGGGCT CCCTGGAAAA 
GATGGCTTGC CTGGGATGAT TGGCAGTCCA GGCTTACCTG GTTCCAAGGG AGCCACTGGT 
GACATCTTTG GTGCTGAAAA TGGTGCTCCG GGGGAACAAG GCCTACAAGG ATTAACAGGG 
CACAAAGGAT TTCTTGGAGA CTCTGGCCTT CCAGGACTCA AGGGTGTGCA CGGGAAGCCT 
GGCTTACTAG GCCCCAAAGG TGAGCGGGGC AGCCCTGGGA CACCAGGACA GGTGGGACAG 
CCAGGCACCC CAGGATCTAG TGGTCCATAT GGCATCAAGG GCAAATCTGG GCTCCCAGGA 
GCACCAGGCT TCCCAGGCAT CTCAGGACAT CCTGGAAAGA AAGGAACAAG AGGCAAGAAA 
GGTCCTCCTG GATCAATTGT AAAGAAAGGG CTGCCAGGGC TAAAAGGCCT TCCTGGAAAT 
CCAGGCCTAG TAGGACTGAA AGGAAGCCCA GGCTCTCCAG GGGTCGCTGG GTTGCCAGCC 
CTCTCTGGAC CCAAGGGAGA GAAGGGGTCT GTTGGATTCG TAGGTTTTCC AGGAATACCA 
GGTCTGCCTG GTATTCCTGG AACAAGAGGA TTAAAAGGAA TTCCAGGATC AACTGGAAAA 
ATGGGACCAT CTGGACGTGC TGGTACTCCT GGTGAAAAGG GAGACAGAGG CAATCCGGGG 
CCAGTCGGAA TACCTAGTCC AAGACGTCCA ATGTCAAACC TTTGGCTCAA AGGAGACAAA 
GGCTCTCAAG GCTCAGCCGG ATCCAATGGA TTTCCTGGGC CAAGAGGTGA CAAAGGAGAG 
GCTGGTCGAC CTGGACCACC AGGCCTACCT GGAGCTCCTG GCCTCCCAGG CATTATCAAA 
GGAGTTAGTG GAAAGCCAGG GCCCCCTGGC TTCATGGGAA TCCGGGGCTT ACCTGGCCTG 
AAGGGGTCCT CTGGGATCAC AGGTTTCCCA GGAATGCCAG GAGAAAGTGG TTCACAAGGT 
ATCAGAGGGT CGCCTGGACT CCCAGGAGCA TCTGGTCTCC CAGGCCTGAA AGGAGACAAC 
GGCCAGACAG TTGAAATTTC CGGTAGCCCA GGACCCAAGG GACAGCCTGG CGAATCTGGT 
TTTAAAGGCA CAAAAGGAAG AGATGGACTA ATAGGCAATA TAGGCTTCCC TGGAAACAAA 
GGTGAAGATG GAAAAGTTGG TGTTTCTGGA GATGTTGGCC TTCCTGGAGC TCCAGGATTT 
CCAGGAGTTG CCGGCATGAG AGGAGAACCA GGACTTCCAG GTTCTTCTGG TCACCAAGGG 
GCAATTGGGC CTCTAGGATC CCCCGGATTA ATAGGACCCA AAGGCTTCCC TGGATTTCCT 
GGTTTACATG GACTGAATGG GCTTCCGGGC ACCAAGGGTA CCCATGGCAC TCCAGGACCT 
AGTATCACCG GTGTGCCTGG GCCTGCTGGT CTCCCTGGAC CCAAAGGAGA AAAAGGATAT 
CCAGGAATTG GCATCGGAGC TCCAGGGAAG CCGGGCCTGA GAGGGCAAAA AGGTGATCGA 
GGTTTCCCAG GTCTCCAGGG CCCTGCTGGT CTCCCCGGTG CCCCAGGCAT CTCCTTGCCC 
TCACTCATAG CAGGACAGCC TGGTGACCCC GGGCGACCAG GCCTAGATGG AGAACGAGGC 
CGCCCAGGCC CCGCTGGACC CCCAGGTCCC CCTGGGCCAT CCTCGAATCA AGGCGACACC 
GGAGACCCTG GCTTCCCTGG AATTCCTGGA CCTAAAGGGC CTAAGGGAGA CCAAGGAATT 
CCAGGTTTTT CTGGCCTCCC TGGAGAGCTA GGACTGAAAG GCATGAGAGG TGAGCCTGGC 
TTCATGGGGA CTCCAGGCAA GGTTGGGCCA CCTGGAGACC CAGGATTTCC CGGAATGAAG 
GGGAAGGCAG GGCCAAGAGG CTCTTCTGGC CTCCAAGGTG ATCCTGGACA AACACCAACT 
GCAGAAGCTG TCCAGGTTCC TCCTGGACCC TTGGGTCTAC CAGGGATCGA TGGCATCCCT 
GGCCTCACTG GGGACCCTGG GGCTCAAGGC CCTGTAGGCC TACAAGGCTC CAAAGGTTTA 
CCTGGCATCC CCGGTAAAGA TGGCCCCAGT GGGCTCCCAG GCCCACCTGG GGCTCTTGGT 
GATCCTGGTC TGCCTGGACT GCAAGGCCCT CCAGGATTTG AAGGAGCTCC AGGGCAGCAA 
GGCCCCTTCG GGATGCCTGG AATGCCTGGC CAGAGCATGA GAGTGGGCTA CACGTTGGTA 
AAGCACAGCC AGTCGGAACA GGTGCCCCCG TGTCCCATCG GGATGAGCCA GCTGTGGGTG 
GGGTACAGCT TACTGTTTGT GGAGGGGCAA GAGAAAGCCC ACAACCAGGA CCTGGGCTTT 
GCTGGCTCCT GTCTGCCCCG CTTCAGCACC ATGCCCTTCA TCTACTGCAA CATCAACGAG 
GTGTGCCACT ATGCCAGGCG CAATGATAAA TCTTACTGGC TCTCCACTAC CGCCCCTATC 
CCCATGATGC CCGTCAGCCA GACCCAGATT CCCCAGTACA TCAGCCGCTG CTCTGTGTGT 
GAGGCACCCT CGCAAGCCAT TGCTGTGCAC AGCCAGGACA TCACCATCCC GCAGTGCCCC 
CTGGGCTGGC GCAGCCTCTG GATTGGGTAC TCTTTCCTCA TGCACACTGC CGCTGGTGCC 
GAGGGTGGAG GCCAGTCCCT GGTCTCACCT GGCTCCTGCC TAGAGGACTT TCGGGCCACT 
CCTTTCATCG AATGCAGTGG TGCCCGAGGC ACCTGCCACT ACTTTGCAAA CAAGTACAGT 
TTCTGGTTGA CCACAGTGGA GGAGAGGCAG CAGTTTGGGG AGTTGCCTGT GTCTGAAACG 
CTGAAAGCTG GGCAGCTCCA CACTCGAGTC AGTCGCTGCC AGGTGTGTAT GAAAAGCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529155.1
CDS	928..5970
Translation	MSATRCSDGVLGSANEDELLQGRPGPIGIQGPTGPQGFTGSTGLSGLKGERGFPGLLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYPGLLGPPGLPGQKGSKGDPVLAPGSFKGMKGDPGLPGLDGITGPQGAPGFPGAVGPAGPPGLQGPPGPPGPLGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPKGFPGISGPPGFPGLGTTGEKGEKGEKGIPGLPGPRGPMGSEGVQGPPGQQGKKGTLGFPGLNGFQGIEGQKGDIGLPGPDVFIDIDGAVISGNPGDPGVPGLPGLKGDEGIQGLRGPSGVPGLPALSGVPGALGPQGFPGLKGDQGNPGRTTIGAAGLPGRDGLPGPPGPPGPPSPEFETETLHNKESGFPGLRGEQGPKGNLGLKGIKGDSGFCACDGGVPNTGPPGEPGPPGPWGLIGLPGLKGARGDRGSGGAQGPAGAPGLVGPLGPSGPKGKKGEPILSTIQGMPGDRGDSGSQGFRGVIGEPGKDGVPGLPGLPGLPGDGGQGFPGEKGLPGLPGEKGHPGPPGLPGNGLPGLPGPRGLPGDKGKDGLPGQQGLPGSKGDCCCREVGKGDLDTERGITLPCIIPGSYGPSGFPGTPGFPGPKGSRGLPGTPGQPGSSGSKGEPGSPGLVHLPELPGFPGPRGEKGLPGFPGLPGKDGLPGMIGSPGLPGSKGATGDIFGAENGAPGEQGLQGLTGHKGFLGDSGLPGLKGVHGKPGLLGPKGERGSPGTPGQVGQPGTPGSSGPYGIKGKSGLPGAPGFPGISGHPGKKGTRGKKGPPGSIVKKGLPGLKGLPGNPGLVGLKGSPGSPGVAGLPALSGPKGEKGSVGFVGFPGIPGLPGIPGTRGLKGIPGSTGKMGPSGRAGTPGEKGDRGNPGPVGIPSPRRPMSNLWLKGDKGSQGSAGSNGFPGPRGDKGEAGRPGPPGLPGAPGLPGIIKGVSGKPGPPGFMGIRGLPGLKGSSGITGFPGMPGESGSQGIRGSPGLPGASGLPGLKGDNGQTVEISGSPGPKGQPGESGFKGTKGRDGLIGNIGFPGNKGEDGKVGVSGDVGLPGAPGFPGVAGMRGEPGLPGSSGHQGAIGPLGSPGLIGPKGFPGFPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGYPGIGIGAPGKPGLRGQKGDRGFPGLQGPAGLPGAPGISLPSLIAGQPGDPGRPGLDGERGRPGPAGPPGPPGPSSNQGDTGDPGFPGIPGPKGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGFPGMKGKAGPRGSSGLQGDPGQTPTAEAVQVPPGPLGLPGIDGIPGLTGDPGAQGPVGLQGSKGLPGIPGKDGPSGLPGPPGALGDPGLPGLQGPPGFEGAPGQQGPFGMPGMPGQSMRVGYTLVKHSQSEQVPPCPIGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVSQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGELPVSETLKAGQLHTRVSRCQVCMKSL

Target ORF information:

RefSeq Version	XM_011530853.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530853.3

ORF Insert Sequence:

ATGTCTGCTA CTAGGTGCAG TGATGGAGTC CTGGGATCTG CCAACGAAGA TGAGCTCTTG 
CAGGGACGAC CTGGACCAAT TGGAATTCAA GGCCCAACAG GTCCTCAAGG ATTCACTGGC 
TCTACTGGTT TATCGGGATT GAAAGGAGAA AGGGGTTTCC CAGGCCTTCT GGGACCTTAT 
GGACCAAAAG GAGATAAGGG TCCCATGGGA GTTCCTGGCT TTCTTGGCAT CAATGGGATT 
CCGGGCCACC CTGGACAACC AGGCCCCAGA GGCCCACCTG GTCTGGATGG CTGTAATGGA 
ACTCAAGGAG CTGTTGGATT TCCAGGCCCT GATGGCTATC CTGGGCTTCT CGGACCACCC 
GGGCTTCCTG GTCAGAAAGG ATCAAAAGGT GACCCTGTCC TTGCTCCAGG TAGTTTCAAA 
GGAATGAAGG GGGATCCTGG GCTGCCTGGA CTGGATGGAA TCACTGGCCC ACAAGGAGCA 
CCCGGATTTC CTGGAGCTGT AGGACCTGCA GGACCACCAG GATTACAAGG TCCTCCAGGG 
CCTCCTGGTC CTCTTGGTCC TGATGGGAAT ATGGGGCTAG GTTTTCAAGG AGAGAAAGGA 
GTCAAGGGGG ATGTTGGCCT CCCTGGCCCA GCAGGACCTC CACCATCTAC TGGAGAGCTG 
GAATTCATGG GATTCCCCAA AGGGAAGAAA GGATCCAAGG GTGAACCAGG GCCTAAGGGT 
TTTCCAGGCA TAAGTGGCCC TCCAGGCTTC CCGGGCCTTG GAACTACTGG AGAAAAGGGA 
GAAAAGGGAG AAAAGGGAAT CCCTGGTTTG CCAGGACCTA GGGGTCCCAT GGGTTCAGAA 
GGAGTCCAAG GCCCTCCAGG GCAACAGGGC AAGAAAGGGA CCCTGGGATT TCCTGGGCTT 
AATGGATTCC AAGGAATTGA GGGTCAAAAG GGTGACATTG GCCTGCCAGG CCCAGATGTT 
TTCATCGATA TAGATGGTGC TGTGATCTCA GGTAATCCTG GAGATCCTGG TGTACCTGGC 
CTCCCAGGCC TTAAAGGAGA TGAAGGCATC CAAGGCCTAC GTGGCCCTTC TGGTGTCCCT 
GGATTGCCAG CATTATCAGG TGTCCCAGGA GCCCTAGGGC CTCAGGGATT TCCAGGGCTG 
AAGGGGGACC AAGGAAACCC AGGCCGTACC ACAATTGGAG CAGCTGGCCT CCCTGGCAGA 
GATGGTTTGC CAGGCCCACC AGGTCCACCA GGCCCACCTA GTCCAGAATT TGAGACTGAA 
ACTCTACACA ACAAAGAGTC AGGGTTCCCT GGTCTCCGAG GAGAACAAGG TCCAAAAGGA 
AACCTAGGCC TCAAAGGAAT AAAAGGAGAC TCAGGTTTCT GTGCTTGTGA CGGTGGTGTT 
CCCAACACTG GACCACCCGG GGAACCAGGC CCACCTGGTC CATGGGGTCT CATAGGCCTT 
CCAGGCCTTA AAGGAGCCAG AGGAGATCGA GGCTCTGGGG GTGCACAGGG CCCAGCAGGG 
GCTCCAGGCT TAGTTGGGCC TCTGGGTCCT TCAGGACCCA AAGGAAAGAA GGGGGAACCA 
ATTCTCAGTA CAATCCAAGG AATGCCAGGA GATCGGGGTG ATTCTGGCTC CCAGGGCTTC 
CGTGGTGTAA TAGGAGAACC AGGCAAGGAC GGAGTACCAG GTTTACCAGG TCTGCCAGGC 
CTTCCGGGTG ATGGTGGACA GGGCTTCCCA GGTGAAAAGG GGTTACCTGG ACTTCCTGGT 
GAAAAAGGCC ATCCTGGTCC ACCTGGCCTC CCAGGAAATG GGTTACCAGG ACTTCCTGGA 
CCCCGTGGGC TTCCTGGAGA TAAAGGCAAG GATGGATTAC CGGGACAACA AGGCCTTCCC 
GGATCTAAGG GTGACTGCTG CTGTAGAGAG GTTGGTAAAG GAGACTTAGA CACAGAGAGA 
GGAATCACCC TGCCCTGTAT TATTCCTGGG TCATACGGTC CATCAGGATT TCCAGGCACT 
CCCGGATTCC CAGGCCCTAA AGGGTCTCGA GGCCTCCCTG GGACCCCAGG CCAGCCTGGG 
TCAAGTGGAA GTAAAGGAGA GCCAGGGAGT CCAGGATTGG TTCATCTTCC TGAATTACCA 
GGATTTCCTG GACCTCGTGG GGAGAAGGGC TTGCCTGGGT TTCCTGGGCT CCCTGGAAAA 
GATGGCTTGC CTGGGATGAT TGGCAGTCCA GGCTTACCTG GTTCCAAGGG AGCCACTGGT 
GACATCTTTG GTGCTGAAAA TGGTGCTCCG GGGGAACAAG GCCTACAAGG ATTAACAGGG 
CACAAAGGAT TTCTTGGAGA CTCTGGCCTT CCAGGACTCA AGGGTGTGCA CGGGAAGCCT 
GGCTTACTAG GCCCCAAAGG TGAGCGGGGC AGCCCTGGGA CACCAGGACA GGTGGGACAG 
CCAGGCACCC CAGGATCTAG TGGTCCATAT GGCATCAAGG GCAAATCTGG GCTCCCAGGA 
GCACCAGGCT TCCCAGGCAT CTCAGGACAT CCTGGAAAGA AAGGAACAAG AGGCAAGAAA 
GGTCCTCCTG GATCAATTGT AAAGAAAGGG CTGCCAGGGC TAAAAGGCCT TCCTGGAAAT 
CCAGGCCTAG TAGGACTGAA AGGAAGCCCA GGCTCTCCAG GGGTCGCTGG GTTGCCAGCC 
CTCTCTGGAC CCAAGGGAGA GAAGGGGTCT GTTGGATTCG TAGGTTTTCC AGGAATACCA 
GGTCTGCCTG GTATTCCTGG AACAAGAGGA TTAAAAGGAA TTCCAGGATC AACTGGAAAA 
ATGGGACCAT CTGGACGTGC TGGTACTCCT GGTGAAAAGG GAGACAGAGG CAATCCGGGG 
CCAGTCGGAA TACCTAGTCC AAGACGTCCA ATGTCAAACC TTTGGCTCAA AGGAGACAAA 
GGCTCTCAAG GCTCAGCCGG ATCCAATGGA TTTCCTGGGC CAAGAGGTGA CAAAGGAGAG 
GCTGGTCGAC CTGGACCACC AGGCCTACCT GGAGCTCCTG GCCTCCCAGG CATTATCAAA 
GGAGTTAGTG GAAAGCCAGG GCCCCCTGGC TTCATGGGAA TCCGGGGCTT ACCTGGCCTG 
AAGGGGTCCT CTGGGATCAC AGGTTTCCCA GGAATGCCAG GAGAAAGTGG TTCACAAGGT 
ATCAGAGGGT CGCCTGGACT CCCAGGAGCA TCTGGTCTCC CAGGCCTGAA AGGAGACAAC 
GGCCAGACAG TTGAAATTTC CGGTAGCCCA GGACCCAAGG GACAGCCTGG CGAATCTGGT 
TTTAAAGGCA CAAAAGGAAG AGATGGACTA ATAGGCAATA TAGGCTTCCC TGGAAACAAA 
GGTGAAGATG GAAAAGTTGG TGTTTCTGGA GATGTTGGCC TTCCTGGAGC TCCAGGATTT 
CCAGGAGTTG CCGGCATGAG AGGAGAACCA GGACTTCCAG GTTCTTCTGG TCACCAAGGG 
GCAATTGGGC CTCTAGGATC CCCCGGATTA ATAGGACCCA AAGGCTTCCC TGGATTTCCT 
GGTTTACATG GACTGAATGG GCTTCCGGGC ACCAAGGGTA CCCATGGCAC TCCAGGACCT 
AGTATCACCG GTGTGCCTGG GCCTGCTGGT CTCCCTGGAC CCAAAGGAGA AAAAGGATAT 
CCAGGAATTG GCATCGGAGC TCCAGGGAAG CCGGGCCTGA GAGGGCAAAA AGGTGATCGA 
GGTTTCCCAG GTCTCCAGGG CCCTGCTGGT CTCCCCGGTG CCCCAGGCAT CTCCTTGCCC 
TCACTCATAG CAGGACAGCC TGGTGACCCC GGGCGACCAG GCCTAGATGG AGAACGAGGC 
CGCCCAGGCC CCGCTGGACC CCCAGGTCCC CCTGGGCCAT CCTCGAATCA AGGCGACACC 
GGAGACCCTG GCTTCCCTGG AATTCCTGGA CCTAAAGGGC CTAAGGGAGA CCAAGGAATT 
CCAGGTTTTT CTGGCCTCCC TGGAGAGCTA GGACTGAAAG GCATGAGAGG TGAGCCTGGC 
TTCATGGGGA CTCCAGGCAA GGTTGGGCCA CCTGGAGACC CAGGATTTCC CGGAATGAAG 
GGGAAGGCAG GGCCAAGAGG CTCTTCTGGC CTCCAAGGTG ATCCTGGACA AACACCAACT 
GCAGAAGCTG TCCAGGTTCC TCCTGGACCC TTGGGTCTAC CAGGGATCGA TGGCATCCCT 
GGCCTCACTG GGGACCCTGG GGCTCAAGGC CCTGTAGGCC TACAAGGCTC CAAAGGTTTA 
CCTGGCATCC CCGGTAAAGA TGGCCCCAGT GGGCTCCCAG GCCCACCTGG GGCTCTTGGT 
GATCCTGGTC TGCCTGGACT GCAAGGCCCT CCAGGATTTG AAGGAGCTCC AGGGCAGCAA 
GGCCCCTTCG GGATGCCTGG AATGCCTGGC CAGAGCATGA GAGTGGGCTA CACGTTGGTA 
AAGCACAGCC AGTCGGAACA GGTGCCCCCG TGTCCCATCG GGATGAGCCA GCTGTGGGTG 
GGGTACAGCT TACTGTTTGT GGAGGGGCAA GAGAAAGCCC ACAACCAGGA CCTGGGCTTT 
GCTGGCTCCT GTCTGCCCCG CTTCAGCACC ATGCCCTTCA TCTACTGCAA CATCAACGAG 
GTGTGCCACT ATGCCAGGCG CAATGATAAA TCTTACTGGC TCTCCACTAC CGCCCCTATC 
CCCATGATGC CCGTCAGCCA GACCCAGATT CCCCAGTACA TCAGCCGCTG CTCTGTGTGT 
GAGGCACCCT CGCAAGCCAT TGCTGTGCAC AGCCAGGACA TCACCATCCC GCAGTGCCCC 
CTGGGCTGGC GCAGCCTCTG GATTGGGTAC TCTTTCCTCA TGCACACTGC CGCTGGTGCC 
GAGGGTGGAG GCCAGTCCCT GGTCTCACCT GGCTCCTGCC TAGAGGACTT TCGGGCCACT 
CCTTTCATCG AATGCAGTGG TGCCCGAGGC ACCTGCCACT ACTTTGCAAA CAAGTACAGT 
TTCTGGTTGA CCACAGTGGA GGAGAGGCAG CAGTTTGGGG AGTTGCCTGT GTCTGAAACG 
CTGAAAGCTG GGCAGCTCCA CACTCGAGTC AGTCGCTGCC AGGTGTGTAT GAAAAGCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( XM_011530854.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529156.1 Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant X4, mRNA.

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CloneID	OHu56984
Clone ID Related Accession (Same CDS sequence)	XM_011530854.2
Accession Version	XM_011530854.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-6(IV) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTATAA ACAAGTTGTG GCTGCTCCTG GTTACGTTGT GCCTGACCGA GGAACTGGCA 
GCAGCGGGAG AGAAGTCTTA TGGAAAGCCA TGTGGGGGCC AGGACTGCAG TGGGAGCTGT 
CAGTGTTTTC CTGAGAAAGG AGCGAGAGGA CGACCTGGAC CAATTGGAAT TCAAGGCCCA 
ACAGGTCCTC AAGGATTCAC TGGCTCTACT GGTTTATCGG GATTGAAAGG AGAAAGGGGT 
TTCCCAGGCC TTCTGGGACC TTATGGACCA AAAGGAGATA AGGGTCCCAT GGGAGTTCCT 
GGCTTTCTTG GCATCAATGG GATTCCGGGC CACCCTGGAC AACCAGGCCC CAGAGGCCCA 
CCTGGTCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATCCTGGGC TTCTCGGACC ACCCGGGCTT CCTGGTCAGA AAGGATCAAA AGGTGACCCT 
GTCCTTGCTC CAGGTAGTTT CAAAGGAATG AAGGGGGATC CTGGGCTGCC TGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TTTCCTGGAG CTGTAGGACC TGCAGGACCA 
CCAGGATTAC AAGGTCCTCC AGGGCCTCCT GGTCCTCTTG GTCCTGATGG GAATATGGGG 
CTAGGTTTTC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCTGG CCCAGCAGGA 
CCTCCACCAT CTACTGGAGA GCTGGAATTC ATGGGATTCC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTAA GGGTTTTCCA GGCATAAGTG GCCCTCCAGG CTTCCCGGGC 
CTTGGAACTA CTGGAGAAAA GGGAGAAAAG GGAGAAAAGG GAATCCCTGG TTTGCCAGGA 
CCTAGGGGTC CCATGGGTTC AGAAGGAGTC CAAGGCCCTC CAGGGCAACA GGGCAAGAAA 
GGGACCCTGG GATTTCCTGG GCTTAATGGA TTCCAAGGAA TTGAGGGTCA AAAGGGTGAC 
ATTGGCCTGC CAGGCCCAGA TGTTTTCATC GATATAGATG GTGCTGTGAT CTCAGGTAAT 
CCTGGAGATC CTGGTGTACC TGGCCTCCCA GGCCTTAAAG GAGATGAAGG CATCCAAGGC 
CTACGTGGCC CTTCTGGTGT CCCTGGATTG CCAGCATTAT CAGGTGTCCC AGGAGCCCTA 
GGGCCTCAGG GATTTCCAGG GCTGAAGGGG GACCAAGGAA ACCCAGGCCG TACCACAATT 
GGAGCAGCTG GCCTCCCTGG CAGAGATGGT TTGCCAGGCC CACCAGGTCC ACCAGGCCCA 
CCTAGTCCAG AATTTGAGAC TGAAACTCTA CACAACAAAG AGTCAGGGTT CCCTGGTCTC 
CGAGGAGAAC AAGGTCCAAA AGGAAACCTA GGCCTCAAAG GAATAAAAGG AGACTCAGGT 
TTCTGTGCTT GTGACGGTGG TGTTCCCAAC ACTGGACCAC CCGGGGAACC AGGCCCACCT 
GGTCCATGGG GTCTCATAGG CCTTCCAGGC CTTAAAGGAG CCAGAGGAGA TCGAGGCTCT 
GGGGGTGCAC AGGGCCCAGC AGGGGCTCCA GGCTTAGTTG GGCCTCTGGG TCCTTCAGGA 
CCCAAAGGAA AGAAGGGGGA ACCAATTCTC AGTACAATCC AAGGAATGCC AGGAGATCGG 
GGTGATTCTG GCTCCCAGGG CTTCCGTGGT GTAATAGGAG AACCAGGCAA GGACGGAGTA 
CCAGGTTTAC CAGGTCTGCC AGGCCTTCCG GGTGATGGTG GACAGGGCTT CCCAGGTGAA 
AAGGGGTTAC CTGGACTTCC TGGTGAAAAA GGCCATCCTG GTCCACCTGG CCTCCCAGGA 
AATGGGTTAC CAGGACTTCC TGGACCCCGT GGGCTTCCTG GAGATAAAGG CAAGGATGGA 
TTACCGGGAC AACAAGGCCT TCCCGGATCT AAGGGTGACT GCTGCTGTAG AGAGGTTGGT 
AAAGGAGACT TAGACACAGA GAGAGGAATC ACCCTGCCCT GTATTATTCC TGGGTCATAC 
GGTCCATCAG GATTTCCAGG CACTCCCGGA TTCCCAGGCC CTAAAGGGTC TCGAGGCCTC 
CCTGGGACCC CAGGCCAGCC TGGGTCAAGT GGAAGTAAAG GAGAGCCAGG GAGTCCAGGA 
TTGGTTCATC TTCCTGAATT ACCAGGATTT CCTGGACCTC GTGGGGAGAA GGGCTTGCCT 
GGGTTTCCTG GGCTCCCTGG AAAAGATGGC TTGCCTGGGA TGATTGGCAG TCCAGGCTTA 
CCTGGTTCCA AGGGAGCCAC TGGTGACATC TTTGGTGCTG AAAATGGTGC TCCGGGGGAA 
CAAGGCCTAC AAGGATTAAC AGGGCACAAA GGATTTCTTG GAGACTCTGG CCTTCCAGGA 
CTCAAGGGTG TGCACGGGAA GCCTGGCTTA CTAGGCCCCA AAGGTGAGCG GGGCAGCCCT 
GGGACACCAG GACAGGTGGG ACAGCCAGGC ACCCCAGGAT CTAGTGGTCC ATATGGCATC 
AAGGGCAAAT CTGGGCTCCC AGGAGCACCA GGCTTCCCAG GCATCTCAGG ACATCCTGGA 
AAGAAAGGAA CAAGAGGCAA GAAAGGTCCT CCTGGATCAA TTGTAAAGAA AGGGCTGCCA 
GGGCTAAAAG GCCTTCCTGG AAATCCAGGC CTAGTAGGAC TGAAAGGAAG CCCAGGCTCT 
CCAGGGGTCG CTGGGTTGCC AGCCCTCTCT GGACCCAAGG GAGAGAAGGG GTCTGTTGGA 
TTCGTAGGTT TTCCAGGAAT ACCAGGTCTG CCTGGTATTC CTGGAACAAG AGGATTAAAA 
GGAATTCCAG GATCAACTGG AAAAATGGGA CCATCTGGAC GTGCTGGTAC TCCTGGTGAA 
AAGGGAGACA GAGGCAATCC GGGGCCAGTC GGAATACCTA GTCCAAGACG TCCAATGTCA 
AACCTTTGGC TCAAAGGAGA CAAAGGCTCT CAAGGCTCAG CCGGATCCAA TGGATTTCCT 
GGGCCAAGAG GTGACAAAGG AGAGGCTGGT CGACCTGGAC CACCAGGCCT ACCTGGAGCT 
CCTGGCCTCC CAGGCATTAT CAAAGGAGTT AGTGGAAAGC CAGGGCCCCC TGGCTTCATG 
GGAATCCGGG GCTTACCTGG CCTGAAGGGG TCCTCTGGGA TCACAGGTTT CCCAGGAATG 
CCAGGAGAAA GTGGTTCACA AGGTATCAGA GGGTCGCCTG GACTCCCAGG AGCATCTGGT 
CTCCCAGGCC TGAAAGGAGA CAACGGCCAG ACAGTTGAAA TTTCCGGTAG CCCAGGACCC 
AAGGGACAGC CTGGCGAATC TGGTTTTAAA GGCACAAAAG GAAGAGATGG ACTAATAGGC 
AATATAGGCT TCCCTGGAAA CAAAGGTGAA GATGGAAAAG TTGGTGTTTC TGGAGATGTT 
GGCCTTCCTG GAGCTCCAGG ATTTCCAGGA GTTGCCGGCA TGAGAGGAGA ACCAGGACTT 
CCAGGTTCTT CTGGTCACCA AGGGGCAATT GGGCCTCTAG GATCCCCCGG ATTAATAGGA 
CCCAAAGGCT TCCCTGGATT TCCTGGTTTA CATGGACTGA ATGGGCTTCC GGGCACCAAG 
GGTACCCATG GCACTCCAGG ACCTAGTATC ACCGGTGTGC CTGGGCCTGC TGGTCTCCCT 
GGACCCAAAG GAGAAAAAGG ATATCCAGGA ATTGGCATCG GAGCTCCAGG GAAGCCGGGC 
CTGAGAGGGC AAAAAGGTGA TCGAGATTTT TACCCTGGGG ATAAAGAAAA ATATTTCAAA 
GGTTCACCAA ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529156.1
CDS	122..3922
Translation	MLINKLWLLLVTLCLTEELAAAGEKSYGKPCGGQDCSGSCQCFPEKGARGRPGPIGIQGPTGPQGFTGSTGLSGLKGERGFPGLLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYPGLLGPPGLPGQKGSKGDPVLAPGSFKGMKGDPGLPGLDGITGPQGAPGFPGAVGPAGPPGLQGPPGPPGPLGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPKGFPGISGPPGFPGLGTTGEKGEKGEKGIPGLPGPRGPMGSEGVQGPPGQQGKKGTLGFPGLNGFQGIEGQKGDIGLPGPDVFIDIDGAVISGNPGDPGVPGLPGLKGDEGIQGLRGPSGVPGLPALSGVPGALGPQGFPGLKGDQGNPGRTTIGAAGLPGRDGLPGPPGPPGPPSPEFETETLHNKESGFPGLRGEQGPKGNLGLKGIKGDSGFCACDGGVPNTGPPGEPGPPGPWGLIGLPGLKGARGDRGSGGAQGPAGAPGLVGPLGPSGPKGKKGEPILSTIQGMPGDRGDSGSQGFRGVIGEPGKDGVPGLPGLPGLPGDGGQGFPGEKGLPGLPGEKGHPGPPGLPGNGLPGLPGPRGLPGDKGKDGLPGQQGLPGSKGDCCCREVGKGDLDTERGITLPCIIPGSYGPSGFPGTPGFPGPKGSRGLPGTPGQPGSSGSKGEPGSPGLVHLPELPGFPGPRGEKGLPGFPGLPGKDGLPGMIGSPGLPGSKGATGDIFGAENGAPGEQGLQGLTGHKGFLGDSGLPGLKGVHGKPGLLGPKGERGSPGTPGQVGQPGTPGSSGPYGIKGKSGLPGAPGFPGISGHPGKKGTRGKKGPPGSIVKKGLPGLKGLPGNPGLVGLKGSPGSPGVAGLPALSGPKGEKGSVGFVGFPGIPGLPGIPGTRGLKGIPGSTGKMGPSGRAGTPGEKGDRGNPGPVGIPSPRRPMSNLWLKGDKGSQGSAGSNGFPGPRGDKGEAGRPGPPGLPGAPGLPGIIKGVSGKPGPPGFMGIRGLPGLKGSSGITGFPGMPGESGSQGIRGSPGLPGASGLPGLKGDNGQTVEISGSPGPKGQPGESGFKGTKGRDGLIGNIGFPGNKGEDGKVGVSGDVGLPGAPGFPGVAGMRGEPGLPGSSGHQGAIGPLGSPGLIGPKGFPGFPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGYPGIGIGAPGKPGLRGQKGDRDFYPGDKEKYFKGSPNQK

Target ORF information:

RefSeq Version	XM_011530854.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 6 chain (COL4A6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530854.2

ORF Insert Sequence:

ATGCTTATAA ACAAGTTGTG GCTGCTCCTG GTTACGTTGT GCCTGACCGA GGAACTGGCA 
GCAGCGGGAG AGAAGTCTTA TGGAAAGCCA TGTGGGGGCC AGGACTGCAG TGGGAGCTGT 
CAGTGTTTTC CTGAGAAAGG AGCGAGAGGA CGACCTGGAC CAATTGGAAT TCAAGGCCCA 
ACAGGTCCTC AAGGATTCAC TGGCTCTACT GGTTTATCGG GATTGAAAGG AGAAAGGGGT 
TTCCCAGGCC TTCTGGGACC TTATGGACCA AAAGGAGATA AGGGTCCCAT GGGAGTTCCT 
GGCTTTCTTG GCATCAATGG GATTCCGGGC CACCCTGGAC AACCAGGCCC CAGAGGCCCA 
CCTGGTCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATCCTGGGC TTCTCGGACC ACCCGGGCTT CCTGGTCAGA AAGGATCAAA AGGTGACCCT 
GTCCTTGCTC CAGGTAGTTT CAAAGGAATG AAGGGGGATC CTGGGCTGCC TGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TTTCCTGGAG CTGTAGGACC TGCAGGACCA 
CCAGGATTAC AAGGTCCTCC AGGGCCTCCT GGTCCTCTTG GTCCTGATGG GAATATGGGG 
CTAGGTTTTC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCTGG CCCAGCAGGA 
CCTCCACCAT CTACTGGAGA GCTGGAATTC ATGGGATTCC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTAA GGGTTTTCCA GGCATAAGTG GCCCTCCAGG CTTCCCGGGC 
CTTGGAACTA CTGGAGAAAA GGGAGAAAAG GGAGAAAAGG GAATCCCTGG TTTGCCAGGA 
CCTAGGGGTC CCATGGGTTC AGAAGGAGTC CAAGGCCCTC CAGGGCAACA GGGCAAGAAA 
GGGACCCTGG GATTTCCTGG GCTTAATGGA TTCCAAGGAA TTGAGGGTCA AAAGGGTGAC 
ATTGGCCTGC CAGGCCCAGA TGTTTTCATC GATATAGATG GTGCTGTGAT CTCAGGTAAT 
CCTGGAGATC CTGGTGTACC TGGCCTCCCA GGCCTTAAAG GAGATGAAGG CATCCAAGGC 
CTACGTGGCC CTTCTGGTGT CCCTGGATTG CCAGCATTAT CAGGTGTCCC AGGAGCCCTA 
GGGCCTCAGG GATTTCCAGG GCTGAAGGGG GACCAAGGAA ACCCAGGCCG TACCACAATT 
GGAGCAGCTG GCCTCCCTGG CAGAGATGGT TTGCCAGGCC CACCAGGTCC ACCAGGCCCA 
CCTAGTCCAG AATTTGAGAC TGAAACTCTA CACAACAAAG AGTCAGGGTT CCCTGGTCTC 
CGAGGAGAAC AAGGTCCAAA AGGAAACCTA GGCCTCAAAG GAATAAAAGG AGACTCAGGT 
TTCTGTGCTT GTGACGGTGG TGTTCCCAAC ACTGGACCAC CCGGGGAACC AGGCCCACCT 
GGTCCATGGG GTCTCATAGG CCTTCCAGGC CTTAAAGGAG CCAGAGGAGA TCGAGGCTCT 
GGGGGTGCAC AGGGCCCAGC AGGGGCTCCA GGCTTAGTTG GGCCTCTGGG TCCTTCAGGA 
CCCAAAGGAA AGAAGGGGGA ACCAATTCTC AGTACAATCC AAGGAATGCC AGGAGATCGG 
GGTGATTCTG GCTCCCAGGG CTTCCGTGGT GTAATAGGAG AACCAGGCAA GGACGGAGTA 
CCAGGTTTAC CAGGTCTGCC AGGCCTTCCG GGTGATGGTG GACAGGGCTT CCCAGGTGAA 
AAGGGGTTAC CTGGACTTCC TGGTGAAAAA GGCCATCCTG GTCCACCTGG CCTCCCAGGA 
AATGGGTTAC CAGGACTTCC TGGACCCCGT GGGCTTCCTG GAGATAAAGG CAAGGATGGA 
TTACCGGGAC AACAAGGCCT TCCCGGATCT AAGGGTGACT GCTGCTGTAG AGAGGTTGGT 
AAAGGAGACT TAGACACAGA GAGAGGAATC ACCCTGCCCT GTATTATTCC TGGGTCATAC 
GGTCCATCAG GATTTCCAGG CACTCCCGGA TTCCCAGGCC CTAAAGGGTC TCGAGGCCTC 
CCTGGGACCC CAGGCCAGCC TGGGTCAAGT GGAAGTAAAG GAGAGCCAGG GAGTCCAGGA 
TTGGTTCATC TTCCTGAATT ACCAGGATTT CCTGGACCTC GTGGGGAGAA GGGCTTGCCT 
GGGTTTCCTG GGCTCCCTGG AAAAGATGGC TTGCCTGGGA TGATTGGCAG TCCAGGCTTA 
CCTGGTTCCA AGGGAGCCAC TGGTGACATC TTTGGTGCTG AAAATGGTGC TCCGGGGGAA 
CAAGGCCTAC AAGGATTAAC AGGGCACAAA GGATTTCTTG GAGACTCTGG CCTTCCAGGA 
CTCAAGGGTG TGCACGGGAA GCCTGGCTTA CTAGGCCCCA AAGGTGAGCG GGGCAGCCCT 
GGGACACCAG GACAGGTGGG ACAGCCAGGC ACCCCAGGAT CTAGTGGTCC ATATGGCATC 
AAGGGCAAAT CTGGGCTCCC AGGAGCACCA GGCTTCCCAG GCATCTCAGG ACATCCTGGA 
AAGAAAGGAA CAAGAGGCAA GAAAGGTCCT CCTGGATCAA TTGTAAAGAA AGGGCTGCCA 
GGGCTAAAAG GCCTTCCTGG AAATCCAGGC CTAGTAGGAC TGAAAGGAAG CCCAGGCTCT 
CCAGGGGTCG CTGGGTTGCC AGCCCTCTCT GGACCCAAGG GAGAGAAGGG GTCTGTTGGA 
TTCGTAGGTT TTCCAGGAAT ACCAGGTCTG CCTGGTATTC CTGGAACAAG AGGATTAAAA 
GGAATTCCAG GATCAACTGG AAAAATGGGA CCATCTGGAC GTGCTGGTAC TCCTGGTGAA 
AAGGGAGACA GAGGCAATCC GGGGCCAGTC GGAATACCTA GTCCAAGACG TCCAATGTCA 
AACCTTTGGC TCAAAGGAGA CAAAGGCTCT CAAGGCTCAG CCGGATCCAA TGGATTTCCT 
GGGCCAAGAG GTGACAAAGG AGAGGCTGGT CGACCTGGAC CACCAGGCCT ACCTGGAGCT 
CCTGGCCTCC CAGGCATTAT CAAAGGAGTT AGTGGAAAGC CAGGGCCCCC TGGCTTCATG 
GGAATCCGGG GCTTACCTGG CCTGAAGGGG TCCTCTGGGA TCACAGGTTT CCCAGGAATG 
CCAGGAGAAA GTGGTTCACA AGGTATCAGA GGGTCGCCTG GACTCCCAGG AGCATCTGGT 
CTCCCAGGCC TGAAAGGAGA CAACGGCCAG ACAGTTGAAA TTTCCGGTAG CCCAGGACCC 
AAGGGACAGC CTGGCGAATC TGGTTTTAAA GGCACAAAAG GAAGAGATGG ACTAATAGGC 
AATATAGGCT TCCCTGGAAA CAAAGGTGAA GATGGAAAAG TTGGTGTTTC TGGAGATGTT 
GGCCTTCCTG GAGCTCCAGG ATTTCCAGGA GTTGCCGGCA TGAGAGGAGA ACCAGGACTT 
CCAGGTTCTT CTGGTCACCA AGGGGCAATT GGGCCTCTAG GATCCCCCGG ATTAATAGGA 
CCCAAAGGCT TCCCTGGATT TCCTGGTTTA CATGGACTGA ATGGGCTTCC GGGCACCAAG 
GGTACCCATG GCACTCCAGG ACCTAGTATC ACCGGTGTGC CTGGGCCTGC TGGTCTCCCT 
GGACCCAAAG GAGAAAAAGG ATATCCAGGA ATTGGCATCG GAGCTCCAGG GAAGCCGGGC 
CTGAGAGGGC AAAAAGGTGA TCGAGATTTT TACCCTGGGG ATAAAGAAAA ATATTTCAAA 
GGTTCACCAA ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	COL4A6
Entrez Gene ID	1288
Full Name	collagen type IV alpha 6 chain
Synonyms	CXDELq22.3,DELXq22.3,DFNX6
General protein information	Preferred Names collagen type IV alpha 6 chain Names collagen alpha-6(IV) chain collagen IV, alpha-6 polypeptide collagen of basement membrane, alpha-6 collagen, type IV, alpha 6 dJ889N15.4 (Collagen Alpha 6(IV))
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq22.3
Summary	This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene, alpha 5 type IV collagen, so that the gene pair shares a common promoter. Deletions in the alpha 5 gene that extend into the alpha 6 gene result in diffuse leiomyomatosis accompanying the X-linked Alport syndrome caused by the deletion in the alpha 5 gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013].
Disorder MIM:	303631
Disorder Html:	?Deafness, X-linked 6, 300914 (3)

Danio rerio (zebrafish)	col4a6	XP_001921630.3
Xenopus tropicalis (tropical clawed frog)	col4a6	XP_004916918.1
Homo sapiens (human)	COL4A6	NP_001274687.1
	COL4A6	XP_001098448.1
Bos taurus (cattle)	COL4A6	XP_005227954.1
Mus musculus (house mouse)	Col4a6	NP_444415.2
Gallus gallus (chicken)	COL4A6	XP_420322.4
Pan troglodytes (chimpanzee)	COL4A6	XP_521216.4
Canis lupus familiaris (dog)	COL4A6	XP_850954.1
Rattus norvegicus (Norway rat)	Col4a6	XP_006257373.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

COL4A6 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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