COL4A6 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL4A6
Entrez Gene ID	523526
Full Name	collagen type IV alpha 6 chain
General protein information	Preferred Names collagen type IV alpha 6 chain Names collagen alpha-6(IV) chain collagen, type IV, alpha 6
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005227897.3	XP_005227954.1	collagen alpha-6(IV) chain isoform X1
XM_010800459.2	XP_010798761.1	collagen alpha-6(IV) chain isoform X2
XM_024988805.1	XP_024844573.1	collagen alpha-6(IV) chain isoform X2
XM_002699886.5	XP_002699932.2	collagen alpha-6(IV) chain isoform X3
XM_002699886.4	XP_002699932.2	collagen alpha-6(IV) chain isoform X2
XM_601826.8	XP_601826.4	collagen alpha-6(IV) chain isoform X1
XM_005227897.4	XP_005227954.1	collagen alpha-6(IV) chain isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	col4a6	XP_001921630.3
Xenopus tropicalis (tropical clawed frog)	col4a6	XP_004916918.1
Homo sapiens (human)	COL4A6	NP_001274687.1
	COL4A6	XP_001098448.1
Bos taurus (cattle)	COL4A6	XP_005227954.1
Mus musculus (house mouse)	Col4a6	NP_444415.2
Gallus gallus (chicken)	COL4A6	XP_420322.4
Pan troglodytes (chimpanzee)	COL4A6	XP_521216.4
Canis lupus familiaris (dog)	COL4A6	XP_850954.1
Rattus norvegicus (Norway rat)	Col4a6	XP_006257373.1

Gene Ontology

The following COL4A6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa238803	XM_024988805.1 Latest version!	Bos taurus collagen type IV alpha 6 chain (COL4A6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa12979	XM_005227897.4 Latest version!	Bos taurus collagen type IV alpha 6 chain (COL4A6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa12979	XM_601826.8 Latest version!	Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa35386	XM_002699886.5 Latest version!	Bos taurus collagen type IV alpha 6 chain (COL4A6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa35386	XM_010800459.2 Latest version!	Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OBa35386	XM_002699886.4	Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa12979	XM_005227897.3	Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL4A6 ( XM_024988805.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844573.1 Bos taurus collagen type IV alpha 6 chain (COL4A6), transcript variant X2, mRNA.

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CloneID	OBa238803
Clone ID Related Accession (Same CDS sequence)	XM_024988805.1
Accession Version	XM_024988805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-6(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT 
AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA 
ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC 
TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGCCACCC TGGACAGCCA 
GGACCCAGAG GCCCACCTGG CCTGGATGGC TGTAATGGAA CTCAAGGAGC TGTTGGATTT 
CCAGGCCCTG ATGGCTATTC TGGGCTTCCT GGACCACCTG GGCTTCCTGG TCAGAAAGGC 
TCTAAAGGTG AACCTGTCCT TGCCCAAGGT AGTTTCAAAG GAATGAAGGG GGAGTCTGGG 
CTGCCCGGAC TGGATGGAAT CACTGGCCCA CAAGGAGCAC CCGGATCTCC AGGAGCTGTA 
GGACCCAGAG GACCACCAGG ATTGCAAGGT CCTCCTGGCC CCCCTGGTTT CCCTGGTCCT 
GATGGGAATA TGGGGTTAGG TTTCCAAGGA GAGAAAGGAG TCAAGGGAGA TGTTGGCCTG 
CCTGGTCCTG CAGGACCCCC ACCATCTACT GGAGAACTGG AATTCATGGG ATTTCCCAAA 
GGGAAGAAAG GATCCAAGGG TGAACCAGGG CCTCAGGGCT TCCCAGGAGT AAGTGGTCCA 
CCTGGTCTGC CGGGATTTGG AATTGCTGGA GAAAAAGGAG AAAAGGGAAT TCCTGGTTTG 
CCAGGACCTA GGGGTCCCAT GGGTTTAGAA GGAGTCCAAG GCCCTCCGGG GAGTCAGGGC 
AGGAAGGGGT CTTCAGGTTT CCCCGGGCTT AATGGATTCC CAGGAATTAA GGGTGAAAAA 
GGTGGCATTG GCCTGCCAGG CCCAGATATT TTCATCGATA CAGATGGTGC TGTGATCTCA 
GGTTATCCTG GAGACCCTGG TATGCCAGGC CTCCCAGGCC TTAAAGGAGA TGAAGGCATA 
CAGGGCCTAC CTGGCCCTCC TGGCGCCCCT GGATTACCAG CTTTACCAGG TGGCCCCGGT 
GCTCTGGGGC CCCAGGGAGC TCTAGGCCTG AAGGGGGAAC GAGGAAACAC TGGCCGGACC 
ACAACGGGGG CAGCTGGTCT CCCTGGCAGA GATGGTTTGC CAGGCCTGCC AGGCCTACCA 
GGCCCACCTG GTGCAGCATC TGAGACGGGA ACCCTACAGA GCACAGAACC GGGATCGCCG 
GGACTCCGAG GGGAACGAGG TCCAAAAGGA CACCCAGGCC TCAAAGGAAT TAAAGGGGAC 
CCAGGTTTTT GTGCTTGTGA TGGTGGTGTC CCCAACATGG GGCCACCTGG GGAGCCAGGC 
CTGCCTGGGC CACCAGGACT TCTGGGCCTT CCAGGTCTTA AAGGAACCAA AGGTGATCCA 
GGCTCTGGGG GTGCACAGGG CCCATCAGGG GATCCAGGTG TATTTGGGCC TCGAGGTCCA 
ACAGGCCCTA AAGGAAAGAA AGGGGACCCA ACTCTCAGCA CAAGATCAGG GATGCCAGGA 
GAGCAAGGTG ATCCTGGCCC CCAGGGGCTC CCTGGTGAGA CAGGAGCCCC AGGCAAGGAT 
GGAATACCAG GTTTACCAGG GCTGCCAGGC CTTCTGGGTG ATGGTGCACA GGGCTTCCCC 
GGTGAAAAGG GGTTACCAGG ACTTCCTGGT GAAAAAGGCC ACACTGGTCC AACTGGCCCC 
CCAGGAATTG GGCTACCAGG GGCTCCTGGA CCTCGTGGAC TTCCTGGAGA TAAAGGAGTA 
GATGGATTAC CAGGGCAACC AGGCCCCCGT GGAGTTCCAG GTGACTGCTG CTGCAAGGAG 
GAGGTTGGGA AAACAGACTT AGACACAGAG GGAGGTATCA CCTTGCCTTG TATTATTCCT 
GGACCGTATG GTCCATCAGG ACTCCCAGGA GCTCCCGGAT TCCCAGGCCC TAAAGGGGCC 
CGTGGCCTCC CTGGGGCTCC AGGCCAACCT GGATTGCGTG GAAATAAAGG GGACCCCGGA 
AGTCCAGGAT TGGTTCACCT GCCTGAATTG CCAGGGTTTC CTGGACCTCA TGGAGAGAAG 
GGCTTACCTG GGTTTCCTGG GCTCCCTGGA AAAGACGGCT TGCCTGGGAA AGTTGGCAGC 
CCCGGCTTAC CAGGTCCCAA GGGAGCCCCT GGTGACATCC TTGGTGCTGA AAATGGTGCT 
CCGGGGGAGC AAGGCCTACA GGGACTGCCT GGGGACAGAG GATTTCCTGG AGACTCTGGC 
CTTCCAGGAC TCAAGGGTTT GCTTGGGAAG TCGGGCTTGC TCGGCCCCAA AGGTGAGCGG 
GGCAGCCCTG GAATACCAGG CCGTGTGGGT CAGCCAGGAC CCCCAGGCCC TAGTGGTCTA 
TTTGGCATCA AGGGCAAACC CGGGCTCCGC GGACCCCCAG GCTTTCCAGG CATTTCAGGA 
TATCCTGGAA AGAAAGGTCC AAGAGGCGAG ATAGGTCTCC CTGCGTCAGC TGGAAAGAGA 
GGCCTGCCTG GGCTGAAAGG GCTTCCGGGA CTGCCAGGGC CAATTGGCTT CCTGGGGAAC 
TCAGGCTTGC CAGGGGCCAC TGGGTTACCA GGCCTGCCAG GTCTGAAGGG TGAGAAGGGG 
TCTGTTGGAC TGACAGGTTT TCCAGGGATT CCAGGTCTCC CTGGTATTCC TGGAGCAAGT 
GGATTAAAGG GAATTCCTGG GTCGACAGGA AGAGTGGGGC CATCTGGATG GGCTGGTAGC 
CCTGGTGAAA AAGGAGACAG AGGTGACCCA GGCCCCTTTG GGATACCCAG TCATCGACCT 
CCAATGCTGA ACCTCCGCTT CAAAGGAGAC AAAGGTTCTC AGGGCTCACC TGGAGCCATT 
GGATTTCCTG GACCCAGAGG TGACAAAGGA GAGGCTGGCC ACCCCGGGCC ACCAGGCTTG 
CCTGGAGCTC CTGGCTTCCC CAGCCTCATC AAGGGACTTA GTGGGAGACC AGGCCCCCCT 
GGCTCCACAG GACTACGGGG CTTACCTGGC CTGAAGGGGT ACCCTGGAAT TGCAGGTTTC 
CAAGGAATTC CAGGAGAAAG TGGTTCACAG GGTCTCAGTG GAGTTCCCGG GCTACCAGGA 
GCATCTGGTC TCCCAGGTTT AAAAGGAGAT CAAGGCCAGA CACTTGGAAT TTCTGGTAGC 
CCAGGACCCA AGGGACAACC TGGGGAGTCT GGTTTCAAAG GTGTGAAAGG AAAAGACGGA 
CCAGTTGGTG ATGTGGGTTT CCCAGGAAAC AAAGGTGAAG ATGGGAAAGT TGGTATTTCT 
GGAGATGTTG GCCTTCCTGG CTCCCTAGGA CTCCCCGGAG TTGCAGGCAT GAGAGGAAAT 
CCAGGGCTTC CAGGTTCTCC AGGCCACCCA GGGGCAACTG GGCCCCTGGG TCCCCCTGGC 
CTAATAGGAA CCAAAGGTTT CCCTGGACTT CCAGGTTTAC ATGGACTGAA TGGGCTTCCA 
GGAACCAAGG GGACTCATGG AACTCCAGGA CCTAGTATAA CCGGTGTGCC TGGGCCAGCT 
GGCTTGCCTG GTCCCAAAGG GGAAAAAGGT TCTCCAGGAA TTGGCATCGG AGCCCCAGGA 
AAGCCAGGTA TAAGAGGACC CAAAGGTAGT CAAGGTTTTC CAGGTCTCCA GGGCGCCGCT 
GGTCTCCCCG GTGCACTGGG CCTCTCCTTG CCTTCAGTCA TAGAAGGACA GCCTGGTGAC 
CCTGGGCGAC CAGGTCTAGA TGGAGAACGA GGCCGCCCAG GCCCTCCTGG GCCCCCAGGC 
CCCCCTGGGC CTGCCTCAGA TCAAGGCGAC CCTGGAGACC CTGGCTTCCC TGGAATTCCT 
GGCCCTCAAG GGCCCAAAGG AGACCAAGGA ATTCCAGGTT TCTCTGGCCT CCCTGGAGAG 
CTAGGATTGA AAGGCATGAG AGGTGAGCCT GGCTTCATGG GGACTCCAGG CAAGGTTGGA 
CCACCTGGAG ACCCAGGACT TCCCGGGATG AAGGGGAAGG CGGGGCCAAG AGGCTTATCT 
GGCCCTCGAG GTGCTCCTGG ACACACACCC ATTGCAGAAG CCATCCAGGC TCCCCCTGGA 
CCCATGGGTC TTCCAGGCAT TGATGGAATC CCTGGCCTCA TGGGAGACCC TGGGGTCCAA 
GGACCTGTGG GCCTACAAGG ATCTAAAGGT TTACCTGGCA TCCCTGGCAA AGATGGCGCC 
AGTGGACTCC CTGGCCCACC TGGGGCTCTT GGTGATCCTG GTCTCCCTGG ACTACAAGGC 
CCTCCAGGAT TTGAAGGAGC TCCAGGGAAG CCGGGCCCCT TCGGGAGGGC TGGAGTGCCT 
GGACAGAGCC TGAGAGTGGG CTACACCTTG GTGAAGCACA GCCAGTCAGA ACAGGTGCCC 
CTGTGCCCCA GCGGGATGAG CCAGCTCTGG GTGGGTTACA GCCTGCTGTT CGTGGAGGGG 
CAAGAGAAAG CCCACAACCA GGACCTGGGG TTTGCTGGCT CCTGCCTGCC CCGCTTCAGC 
ACCATGCCAT TCATCTACTG CAACATCAAT GAAGTGTGCC ACTATGCCCG ACGGAATGAT 
AAGTCCTACT GGCTCTCCAC CACCGCCCCC ATCCCTATGA TGCCGGTCGG CCAGACACAG 
ATCCCCCAGT ACATCAGCCG CTGCTCTGTG TGTGAGGCGC CCTCGCAAGC CATTGCTGTG 
CACAGCCAGG ACATCACCAT CCCACAGTGC CCCCTGGGCT GGCGCAGTCT GTGGATTGGG 
TACTCCTTCC TCATGCACAC AGCCGCCGGT GCAGAGGGCG GGGGCCAGTC CCTGGTCTCT 
CCCGGATCCT GCCTAGAGGA TTTCCGGGCT ACTCCTTTCA TCGAATGCAG TGGTGCCCGG 
GGCACCTGCC ACTACTTTGC CAACAAATAT AGTTTCTGGC TGACAACAGT GGAAGAGAGG 
CAGCAGTTTG GGGAGGAGCC TGTATCTGAG ACGCTGAAAG CTGGACAGCT CCACACCCGA 
GTCAGCCGCT GCCAGGTGTG TATGAAAAGC GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844573.1
CDS	256..5331
Translation	MHPGLWLLLVTLCLTEELAGAGEKKPYGKPCGGQDCSGGCKCSPEKGARGRPGPIGIQGPTGPQGLPGPSGLSGLKGERGSPGPLGPYGPKGDKGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYSGLPGPPGLPGQKGSKGEPVLAQGSFKGMKGESGLPGLDGITGPQGAPGSPGAVGPRGPPGLQGPPGPPGFPGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPQGFPGVSGPPGLPGFGIAGEKGEKGIPGLPGPRGPMGLEGVQGPPGSQGRKGSSGFPGLNGFPGIKGEKGGIGLPGPDIFIDTDGAVISGYPGDPGMPGLPGLKGDEGIQGLPGPPGAPGLPALPGGPGALGPQGALGLKGERGNTGRTTTGAAGLPGRDGLPGLPGLPGPPGAASETGTLQSTEPGSPGLRGERGPKGHPGLKGIKGDPGFCACDGGVPNMGPPGEPGLPGPPGLLGLPGLKGTKGDPGSGGAQGPSGDPGVFGPRGPTGPKGKKGDPTLSTRSGMPGEQGDPGPQGLPGETGAPGKDGIPGLPGLPGLLGDGAQGFPGEKGLPGLPGEKGHTGPTGPPGIGLPGAPGPRGLPGDKGVDGLPGQPGPRGVPGDCCCKEEVGKTDLDTEGGITLPCIIPGPYGPSGLPGAPGFPGPKGARGLPGAPGQPGLRGNKGDPGSPGLVHLPELPGFPGPHGEKGLPGFPGLPGKDGLPGKVGSPGLPGPKGAPGDILGAENGAPGEQGLQGLPGDRGFPGDSGLPGLKGLLGKSGLLGPKGERGSPGIPGRVGQPGPPGPSGLFGIKGKPGLRGPPGFPGISGYPGKKGPRGEIGLPASAGKRGLPGLKGLPGLPGPIGFLGNSGLPGATGLPGLPGLKGEKGSVGLTGFPGIPGLPGIPGASGLKGIPGSTGRVGPSGWAGSPGEKGDRGDPGPFGIPSHRPPMLNLRFKGDKGSQGSPGAIGFPGPRGDKGEAGHPGPPGLPGAPGFPSLIKGLSGRPGPPGSTGLRGLPGLKGYPGIAGFQGIPGESGSQGLSGVPGLPGASGLPGLKGDQGQTLGISGSPGPKGQPGESGFKGVKGKDGPVGDVGFPGNKGEDGKVGISGDVGLPGSLGLPGVAGMRGNPGLPGSPGHPGATGPLGPPGLIGTKGFPGLPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGSPGIGIGAPGKPGIRGPKGSQGFPGLQGAAGLPGALGLSLPSVIEGQPGDPGRPGLDGERGRPGPPGPPGPPGPASDQGDPGDPGFPGIPGPQGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGLPGMKGKAGPRGLSGPRGAPGHTPIAEAIQAPPGPMGLPGIDGIPGLMGDPGVQGPVGLQGSKGLPGIPGKDGASGLPGPPGALGDPGLPGLQGPPGFEGAPGKPGPFGRAGVPGQSLRVGYTLVKHSQSEQVPLCPSGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVGQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGEEPVSETLKAGQLHTRVSRCQVCMKSV

Target ORF information:

RefSeq Version	XM_024988805.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type IV alpha 6 chain (COL4A6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988805.1

ORF Insert Sequence:

ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT 
AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA 
ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC 
TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGCCACCC TGGACAGCCA 
GGACCCAGAG GCCCACCTGG CCTGGATGGC TGTAATGGAA CTCAAGGAGC TGTTGGATTT 
CCAGGCCCTG ATGGCTATTC TGGGCTTCCT GGACCACCTG GGCTTCCTGG TCAGAAAGGC 
TCTAAAGGTG AACCTGTCCT TGCCCAAGGT AGTTTCAAAG GAATGAAGGG GGAGTCTGGG 
CTGCCCGGAC TGGATGGAAT CACTGGCCCA CAAGGAGCAC CCGGATCTCC AGGAGCTGTA 
GGACCCAGAG GACCACCAGG ATTGCAAGGT CCTCCTGGCC CCCCTGGTTT CCCTGGTCCT 
GATGGGAATA TGGGGTTAGG TTTCCAAGGA GAGAAAGGAG TCAAGGGAGA TGTTGGCCTG 
CCTGGTCCTG CAGGACCCCC ACCATCTACT GGAGAACTGG AATTCATGGG ATTTCCCAAA 
GGGAAGAAAG GATCCAAGGG TGAACCAGGG CCTCAGGGCT TCCCAGGAGT AAGTGGTCCA 
CCTGGTCTGC CGGGATTTGG AATTGCTGGA GAAAAAGGAG AAAAGGGAAT TCCTGGTTTG 
CCAGGACCTA GGGGTCCCAT GGGTTTAGAA GGAGTCCAAG GCCCTCCGGG GAGTCAGGGC 
AGGAAGGGGT CTTCAGGTTT CCCCGGGCTT AATGGATTCC CAGGAATTAA GGGTGAAAAA 
GGTGGCATTG GCCTGCCAGG CCCAGATATT TTCATCGATA CAGATGGTGC TGTGATCTCA 
GGTTATCCTG GAGACCCTGG TATGCCAGGC CTCCCAGGCC TTAAAGGAGA TGAAGGCATA 
CAGGGCCTAC CTGGCCCTCC TGGCGCCCCT GGATTACCAG CTTTACCAGG TGGCCCCGGT 
GCTCTGGGGC CCCAGGGAGC TCTAGGCCTG AAGGGGGAAC GAGGAAACAC TGGCCGGACC 
ACAACGGGGG CAGCTGGTCT CCCTGGCAGA GATGGTTTGC CAGGCCTGCC AGGCCTACCA 
GGCCCACCTG GTGCAGCATC TGAGACGGGA ACCCTACAGA GCACAGAACC GGGATCGCCG 
GGACTCCGAG GGGAACGAGG TCCAAAAGGA CACCCAGGCC TCAAAGGAAT TAAAGGGGAC 
CCAGGTTTTT GTGCTTGTGA TGGTGGTGTC CCCAACATGG GGCCACCTGG GGAGCCAGGC 
CTGCCTGGGC CACCAGGACT TCTGGGCCTT CCAGGTCTTA AAGGAACCAA AGGTGATCCA 
GGCTCTGGGG GTGCACAGGG CCCATCAGGG GATCCAGGTG TATTTGGGCC TCGAGGTCCA 
ACAGGCCCTA AAGGAAAGAA AGGGGACCCA ACTCTCAGCA CAAGATCAGG GATGCCAGGA 
GAGCAAGGTG ATCCTGGCCC CCAGGGGCTC CCTGGTGAGA CAGGAGCCCC AGGCAAGGAT 
GGAATACCAG GTTTACCAGG GCTGCCAGGC CTTCTGGGTG ATGGTGCACA GGGCTTCCCC 
GGTGAAAAGG GGTTACCAGG ACTTCCTGGT GAAAAAGGCC ACACTGGTCC AACTGGCCCC 
CCAGGAATTG GGCTACCAGG GGCTCCTGGA CCTCGTGGAC TTCCTGGAGA TAAAGGAGTA 
GATGGATTAC CAGGGCAACC AGGCCCCCGT GGAGTTCCAG GTGACTGCTG CTGCAAGGAG 
GAGGTTGGGA AAACAGACTT AGACACAGAG GGAGGTATCA CCTTGCCTTG TATTATTCCT 
GGACCGTATG GTCCATCAGG ACTCCCAGGA GCTCCCGGAT TCCCAGGCCC TAAAGGGGCC 
CGTGGCCTCC CTGGGGCTCC AGGCCAACCT GGATTGCGTG GAAATAAAGG GGACCCCGGA 
AGTCCAGGAT TGGTTCACCT GCCTGAATTG CCAGGGTTTC CTGGACCTCA TGGAGAGAAG 
GGCTTACCTG GGTTTCCTGG GCTCCCTGGA AAAGACGGCT TGCCTGGGAA AGTTGGCAGC 
CCCGGCTTAC CAGGTCCCAA GGGAGCCCCT GGTGACATCC TTGGTGCTGA AAATGGTGCT 
CCGGGGGAGC AAGGCCTACA GGGACTGCCT GGGGACAGAG GATTTCCTGG AGACTCTGGC 
CTTCCAGGAC TCAAGGGTTT GCTTGGGAAG TCGGGCTTGC TCGGCCCCAA AGGTGAGCGG 
GGCAGCCCTG GAATACCAGG CCGTGTGGGT CAGCCAGGAC CCCCAGGCCC TAGTGGTCTA 
TTTGGCATCA AGGGCAAACC CGGGCTCCGC GGACCCCCAG GCTTTCCAGG CATTTCAGGA 
TATCCTGGAA AGAAAGGTCC AAGAGGCGAG ATAGGTCTCC CTGCGTCAGC TGGAAAGAGA 
GGCCTGCCTG GGCTGAAAGG GCTTCCGGGA CTGCCAGGGC CAATTGGCTT CCTGGGGAAC 
TCAGGCTTGC CAGGGGCCAC TGGGTTACCA GGCCTGCCAG GTCTGAAGGG TGAGAAGGGG 
TCTGTTGGAC TGACAGGTTT TCCAGGGATT CCAGGTCTCC CTGGTATTCC TGGAGCAAGT 
GGATTAAAGG GAATTCCTGG GTCGACAGGA AGAGTGGGGC CATCTGGATG GGCTGGTAGC 
CCTGGTGAAA AAGGAGACAG AGGTGACCCA GGCCCCTTTG GGATACCCAG TCATCGACCT 
CCAATGCTGA ACCTCCGCTT CAAAGGAGAC AAAGGTTCTC AGGGCTCACC TGGAGCCATT 
GGATTTCCTG GACCCAGAGG TGACAAAGGA GAGGCTGGCC ACCCCGGGCC ACCAGGCTTG 
CCTGGAGCTC CTGGCTTCCC CAGCCTCATC AAGGGACTTA GTGGGAGACC AGGCCCCCCT 
GGCTCCACAG GACTACGGGG CTTACCTGGC CTGAAGGGGT ACCCTGGAAT TGCAGGTTTC 
CAAGGAATTC CAGGAGAAAG TGGTTCACAG GGTCTCAGTG GAGTTCCCGG GCTACCAGGA 
GCATCTGGTC TCCCAGGTTT AAAAGGAGAT CAAGGCCAGA CACTTGGAAT TTCTGGTAGC 
CCAGGACCCA AGGGACAACC TGGGGAGTCT GGTTTCAAAG GTGTGAAAGG AAAAGACGGA 
CCAGTTGGTG ATGTGGGTTT CCCAGGAAAC AAAGGTGAAG ATGGGAAAGT TGGTATTTCT 
GGAGATGTTG GCCTTCCTGG CTCCCTAGGA CTCCCCGGAG TTGCAGGCAT GAGAGGAAAT 
CCAGGGCTTC CAGGTTCTCC AGGCCACCCA GGGGCAACTG GGCCCCTGGG TCCCCCTGGC 
CTAATAGGAA CCAAAGGTTT CCCTGGACTT CCAGGTTTAC ATGGACTGAA TGGGCTTCCA 
GGAACCAAGG GGACTCATGG AACTCCAGGA CCTAGTATAA CCGGTGTGCC TGGGCCAGCT 
GGCTTGCCTG GTCCCAAAGG GGAAAAAGGT TCTCCAGGAA TTGGCATCGG AGCCCCAGGA 
AAGCCAGGTA TAAGAGGACC CAAAGGTAGT CAAGGTTTTC CAGGTCTCCA GGGCGCCGCT 
GGTCTCCCCG GTGCACTGGG CCTCTCCTTG CCTTCAGTCA TAGAAGGACA GCCTGGTGAC 
CCTGGGCGAC CAGGTCTAGA TGGAGAACGA GGCCGCCCAG GCCCTCCTGG GCCCCCAGGC 
CCCCCTGGGC CTGCCTCAGA TCAAGGCGAC CCTGGAGACC CTGGCTTCCC TGGAATTCCT 
GGCCCTCAAG GGCCCAAAGG AGACCAAGGA ATTCCAGGTT TCTCTGGCCT CCCTGGAGAG 
CTAGGATTGA AAGGCATGAG AGGTGAGCCT GGCTTCATGG GGACTCCAGG CAAGGTTGGA 
CCACCTGGAG ACCCAGGACT TCCCGGGATG AAGGGGAAGG CGGGGCCAAG AGGCTTATCT 
GGCCCTCGAG GTGCTCCTGG ACACACACCC ATTGCAGAAG CCATCCAGGC TCCCCCTGGA 
CCCATGGGTC TTCCAGGCAT TGATGGAATC CCTGGCCTCA TGGGAGACCC TGGGGTCCAA 
GGACCTGTGG GCCTACAAGG ATCTAAAGGT TTACCTGGCA TCCCTGGCAA AGATGGCGCC 
AGTGGACTCC CTGGCCCACC TGGGGCTCTT GGTGATCCTG GTCTCCCTGG ACTACAAGGC 
CCTCCAGGAT TTGAAGGAGC TCCAGGGAAG CCGGGCCCCT TCGGGAGGGC TGGAGTGCCT 
GGACAGAGCC TGAGAGTGGG CTACACCTTG GTGAAGCACA GCCAGTCAGA ACAGGTGCCC 
CTGTGCCCCA GCGGGATGAG CCAGCTCTGG GTGGGTTACA GCCTGCTGTT CGTGGAGGGG 
CAAGAGAAAG CCCACAACCA GGACCTGGGG TTTGCTGGCT CCTGCCTGCC CCGCTTCAGC 
ACCATGCCAT TCATCTACTG CAACATCAAT GAAGTGTGCC ACTATGCCCG ACGGAATGAT 
AAGTCCTACT GGCTCTCCAC CACCGCCCCC ATCCCTATGA TGCCGGTCGG CCAGACACAG 
ATCCCCCAGT ACATCAGCCG CTGCTCTGTG TGTGAGGCGC CCTCGCAAGC CATTGCTGTG 
CACAGCCAGG ACATCACCAT CCCACAGTGC CCCCTGGGCT GGCGCAGTCT GTGGATTGGG 
TACTCCTTCC TCATGCACAC AGCCGCCGGT GCAGAGGGCG GGGGCCAGTC CCTGGTCTCT 
CCCGGATCCT GCCTAGAGGA TTTCCGGGCT ACTCCTTTCA TCGAATGCAG TGGTGCCCGG 
GGCACCTGCC ACTACTTTGC CAACAAATAT AGTTTCTGGC TGACAACAGT GGAAGAGAGG 
CAGCAGTTTG GGGAGGAGCC TGTATCTGAG ACGCTGAAAG CTGGACAGCT CCACACCCGA 
GTCAGCCGCT GCCAGGTGTG TATGAAAAGC GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( XM_005227897.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227954.1 Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X1, mRNA.

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CloneID	OBa12979
Clone ID Related Accession (Same CDS sequence)	XM_005227897.3 , XM_601826.8 , XM_005227897.4
Accession Version	XM_005227897.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-6(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104715.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227897.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT 
AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA 
ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC 
TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC 
GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA 
CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT 
GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA 
CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG 
TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA 
CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA 
TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT 
CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA 
GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG 
CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC 
CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC 
CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG 
GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT 
GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA 
GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA 
CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT 
TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA 
GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA 
CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA 
AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT 
GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA 
CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA 
CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA 
CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG 
CAACCAGGCC CCCGTGGAGT TCCAGGTGAC TGCTGCTGCA AGGAGGAGGT TGGGAAAACA 
GACTTAGACA CAGAGGGAGG TATCACCTTG CCTTGTATTA TTCCTGGACC GTATGGTCCA 
TCAGGACTCC CAGGAGCTCC CGGATTCCCA GGCCCTAAAG GGGCCCGTGG CCTCCCTGGG 
GCTCCAGGCC AACCTGGATT GCGTGGAAAT AAAGGGGACC CCGGAAGTCC AGGATTGGTT 
CACCTGCCTG AATTGCCAGG GTTTCCTGGA CCTCATGGAG AGAAGGGCTT ACCTGGGTTT 
CCTGGGCTCC CTGGAAAAGA CGGCTTGCCT GGGAAAGTTG GCAGCCCCGG CTTACCAGGT 
CCCAAGGGAG CCCCTGGTGA CATCCTTGGT GCTGAAAATG GTGCTCCGGG GGAGCAAGGC 
CTACAGGGAC TGCCTGGGGA CAGAGGATTT CCTGGAGACT CTGGCCTTCC AGGACTCAAG 
GGTTTGCTTG GGAAGTCGGG CTTGCTCGGC CCCAAAGGTG AGCGGGGCAG CCCTGGAATA 
CCAGGCCGTG TGGGTCAGCC AGGACCCCCA GGCCCTAGTG GTCTATTTGG CATCAAGGGC 
AAACCCGGGC TCCGCGGACC CCCAGGCTTT CCAGGCATTT CAGGATATCC TGGAAAGAAA 
GGTCCAAGAG GCGAGATAGG TCTCCCTGCG TCAGCTGGAA AGAGAGGCCT GCCTGGGCTG 
AAAGGGCTTC CGGGACTGCC AGGGCCAATT GGCTTCCTGG GGAACTCAGG CTTGCCAGGG 
GCCACTGGGT TACCAGGCCT GCCAGGTCTG AAGGGTGAGA AGGGGTCTGT TGGACTGACA 
GGTTTTCCAG GGATTCCAGG TCTCCCTGGT ATTCCTGGAG CAAGTGGATT AAAGGGAATT 
CCTGGGTCGA CAGGAAGAGT GGGGCCATCT GGATGGGCTG GTAGCCCTGG TGAAAAAGGA 
GACAGAGGTG ACCCAGGCCC CTTTGGGATA CCCAGTCATC GACCTCCAAT GCTGAACCTC 
CGCTTCAAAG GAGACAAAGG TTCTCAGGGC TCACCTGGAG CCATTGGATT TCCTGGACCC 
AGAGGTGACA AAGGAGAGGC TGGCCACCCC GGGCCACCAG GCTTGCCTGG AGCTCCTGGC 
TTCCCCAGCC TCATCAAGGG ACTTAGTGGG AGACCAGGCC CCCCTGGCTC CACAGGACTA 
CGGGGCTTAC CTGGCCTGAA GGGGTACCCT GGAATTGCAG GTTTCCAAGG AATTCCAGGA 
GAAAGTGGTT CACAGGGTCT CAGTGGAGTT CCCGGGCTAC CAGGAGCATC TGGTCTCCCA 
GGTTTAAAAG GAGATCAAGG CCAGACACTT GGAATTTCTG GTAGCCCAGG ACCCAAGGGA 
CAACCTGGGG AGTCTGGTTT CAAAGGTGTG AAAGGAAAAG ACGGACCAGT TGGTGATGTG 
GGTTTCCCAG GAAACAAAGG TGAAGATGGG AAAGTTGGTA TTTCTGGAGA TGTTGGCCTT 
CCTGGCTCCC TAGGACTCCC CGGAGTTGCA GGCATGAGAG GAAATCCAGG GCTTCCAGGT 
TCTCCAGGCC ACCCAGGGGC AACTGGGCCC CTGGGTCCCC CTGGCCTAAT AGGAACCAAA 
GGTTTCCCTG GACTTCCAGG TTTACATGGA CTGAATGGGC TTCCAGGAAC CAAGGGGACT 
CATGGAACTC CAGGACCTAG TATAACCGGT GTGCCTGGGC CAGCTGGCTT GCCTGGTCCC 
AAAGGGGAAA AAGGTTCTCC AGGAATTGGC ATCGGAGCCC CAGGAAAGCC AGGTATAAGA 
GGACCCAAAG GTAGTCAAGG TTTTCCAGGT CTCCAGGGCG CCGCTGGTCT CCCCGGTGCA 
CTGGGCCTCT CCTTGCCTTC AGTCATAGAA GGACAGCCTG GTGACCCTGG GCGACCAGGT 
CTAGATGGAG AACGAGGCCG CCCAGGCCCT CCTGGGCCCC CAGGCCCCCC TGGGCCTGCC 
TCAGATCAAG GCGACCCTGG AGACCCTGGC TTCCCTGGAA TTCCTGGCCC TCAAGGGCCC 
AAAGGAGACC AAGGAATTCC AGGTTTCTCT GGCCTCCCTG GAGAGCTAGG ATTGAAAGGC 
ATGAGAGGTG AGCCTGGCTT CATGGGGACT CCAGGCAAGG TTGGACCACC TGGAGACCCA 
GGACTTCCCG GGATGAAGGG GAAGGCGGGG CCAAGAGGCT TATCTGGCCC TCGAGGTGCT 
CCTGGACACA CACCCATTGC AGAAGCCATC CAGGCTCCCC CTGGACCCAT GGGTCTTCCA 
GGCATTGATG GAATCCCTGG CCTCATGGGA GACCCTGGGG TCCAAGGACC TGTGGGCCTA 
CAAGGATCTA AAGGTTTACC TGGCATCCCT GGCAAAGATG GCGCCAGTGG ACTCCCTGGC 
CCACCTGGGG CTCTTGGTGA TCCTGGTCTC CCTGGACTAC AAGGCCCTCC AGGATTTGAA 
GGAGCTCCAG GGAAGCCGGG CCCCTTCGGG AGGGCTGGAG TGCCTGGACA GAGCCTGAGA 
GTGGGCTACA CCTTGGTGAA GCACAGCCAG TCAGAACAGG TGCCCCTGTG CCCCAGCGGG 
ATGAGCCAGC TCTGGGTGGG TTACAGCCTG CTGTTCGTGG AGGGGCAAGA GAAAGCCCAC 
AACCAGGACC TGGGGTTTGC TGGCTCCTGC CTGCCCCGCT TCAGCACCAT GCCATTCATC 
TACTGCAACA TCAATGAAGT GTGCCACTAT GCCCGACGGA ATGATAAGTC CTACTGGCTC 
TCCACCACCG CCCCCATCCC TATGATGCCG GTCGGCCAGA CACAGATCCC CCAGTACATC 
AGCCGCTGCT CTGTGTGTGA GGCGCCCTCG CAAGCCATTG CTGTGCACAG CCAGGACATC 
ACCATCCCAC AGTGCCCCCT GGGCTGGCGC AGTCTGTGGA TTGGGTACTC CTTCCTCATG 
CACACAGCCG CCGGTGCAGA GGGCGGGGGC CAGTCCCTGG TCTCTCCCGG ATCCTGCCTA 
GAGGATTTCC GGGCTACTCC TTTCATCGAA TGCAGTGGTG CCCGGGGCAC CTGCCACTAC 
TTTGCCAACA AATATAGTTT CTGGCTGACA ACAGTGGAAG AGAGGCAGCA GTTTGGGGAG 
GAGCCTGTAT CTGAGACGCT GAAAGCTGGA CAGCTCCACA CCCGAGTCAG CCGCTGCCAG 
GTGTGTATGA AAAGCGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227954.1
CDS	270..5390
Translation	MHPGLWLLLVTLCLTEELAGAGEKKPYGKPCGGQDCSGGCKCSPEKGARGRPGPIGIQGPTGPQGLPGPSGLSGLKGERGSPGPLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYSGLPGPPGLPGQKGSKGEPVLAQGSFKGMKGESGLPGLDGITGPQGAPGSPGAVGPRGPPGLQGPPGPPGFPGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPQGFPGVSGPPGLPGFGIAGEKGEKGIPGLPGPRGPMGLEGVQGPPGSQGRKGSSGFPGLNGFPGIKGEKGGIGLPGPDIFIDTDGAVISGYPGDPGMPGLPGLKGDEGIQGLPGPPGAPGLPALPGGPGALGPQGALGLKGERGNTGRTTTGAAGLPGRDGLPGLPGLPGPPGAASETGTLQSTEPGSPGLRGERGPKGHPGLKGIKGDPGFCACDGGVPNMGPPGEPGLPGPPGLLGLPGLKGTKGDPGSGGAQGPSGDPGVFGPRGPTGPKGKKGDPTLSTRSGMPGEQGDPGPQGLPGETGAPGKDGIPGLPGLPGLLGDGAQGFPGEKGLPGLPGEKGHTGPTGPPGIGLPGAPGPRGLPGDKGVDGLPGQPGPRGVPGDCCCKEEVGKTDLDTEGGITLPCIIPGPYGPSGLPGAPGFPGPKGARGLPGAPGQPGLRGNKGDPGSPGLVHLPELPGFPGPHGEKGLPGFPGLPGKDGLPGKVGSPGLPGPKGAPGDILGAENGAPGEQGLQGLPGDRGFPGDSGLPGLKGLLGKSGLLGPKGERGSPGIPGRVGQPGPPGPSGLFGIKGKPGLRGPPGFPGISGYPGKKGPRGEIGLPASAGKRGLPGLKGLPGLPGPIGFLGNSGLPGATGLPGLPGLKGEKGSVGLTGFPGIPGLPGIPGASGLKGIPGSTGRVGPSGWAGSPGEKGDRGDPGPFGIPSHRPPMLNLRFKGDKGSQGSPGAIGFPGPRGDKGEAGHPGPPGLPGAPGFPSLIKGLSGRPGPPGSTGLRGLPGLKGYPGIAGFQGIPGESGSQGLSGVPGLPGASGLPGLKGDQGQTLGISGSPGPKGQPGESGFKGVKGKDGPVGDVGFPGNKGEDGKVGISGDVGLPGSLGLPGVAGMRGNPGLPGSPGHPGATGPLGPPGLIGTKGFPGLPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGSPGIGIGAPGKPGIRGPKGSQGFPGLQGAAGLPGALGLSLPSVIEGQPGDPGRPGLDGERGRPGPPGPPGPPGPASDQGDPGDPGFPGIPGPQGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGLPGMKGKAGPRGLSGPRGAPGHTPIAEAIQAPPGPMGLPGIDGIPGLMGDPGVQGPVGLQGSKGLPGIPGKDGASGLPGPPGALGDPGLPGLQGPPGFEGAPGKPGPFGRAGVPGQSLRVGYTLVKHSQSEQVPLCPSGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVGQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGEEPVSETLKAGQLHTRVSRCQVCMKSV

Target ORF information:

RefSeq Version	XM_005227897.3
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227897.3

ORF Insert Sequence:

ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT 
AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA 
ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC 
TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC 
GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA 
CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT 
GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA 
CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG 
TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA 
CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA 
TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT 
CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA 
GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG 
CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC 
CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC 
CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG 
GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT 
GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA 
GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA 
CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT 
TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA 
GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA 
CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA 
AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT 
GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA 
CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA 
CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA 
CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG 
CAACCAGGCC CCCGTGGAGT TCCAGGTGAC TGCTGCTGCA AGGAGGAGGT TGGGAAAACA 
GACTTAGACA CAGAGGGAGG TATCACCTTG CCTTGTATTA TTCCTGGACC GTATGGTCCA 
TCAGGACTCC CAGGAGCTCC CGGATTCCCA GGCCCTAAAG GGGCCCGTGG CCTCCCTGGG 
GCTCCAGGCC AACCTGGATT GCGTGGAAAT AAAGGGGACC CCGGAAGTCC AGGATTGGTT 
CACCTGCCTG AATTGCCAGG GTTTCCTGGA CCTCATGGAG AGAAGGGCTT ACCTGGGTTT 
CCTGGGCTCC CTGGAAAAGA CGGCTTGCCT GGGAAAGTTG GCAGCCCCGG CTTACCAGGT 
CCCAAGGGAG CCCCTGGTGA CATCCTTGGT GCTGAAAATG GTGCTCCGGG GGAGCAAGGC 
CTACAGGGAC TGCCTGGGGA CAGAGGATTT CCTGGAGACT CTGGCCTTCC AGGACTCAAG 
GGTTTGCTTG GGAAGTCGGG CTTGCTCGGC CCCAAAGGTG AGCGGGGCAG CCCTGGAATA 
CCAGGCCGTG TGGGTCAGCC AGGACCCCCA GGCCCTAGTG GTCTATTTGG CATCAAGGGC 
AAACCCGGGC TCCGCGGACC CCCAGGCTTT CCAGGCATTT CAGGATATCC TGGAAAGAAA 
GGTCCAAGAG GCGAGATAGG TCTCCCTGCG TCAGCTGGAA AGAGAGGCCT GCCTGGGCTG 
AAAGGGCTTC CGGGACTGCC AGGGCCAATT GGCTTCCTGG GGAACTCAGG CTTGCCAGGG 
GCCACTGGGT TACCAGGCCT GCCAGGTCTG AAGGGTGAGA AGGGGTCTGT TGGACTGACA 
GGTTTTCCAG GGATTCCAGG TCTCCCTGGT ATTCCTGGAG CAAGTGGATT AAAGGGAATT 
CCTGGGTCGA CAGGAAGAGT GGGGCCATCT GGATGGGCTG GTAGCCCTGG TGAAAAAGGA 
GACAGAGGTG ACCCAGGCCC CTTTGGGATA CCCAGTCATC GACCTCCAAT GCTGAACCTC 
CGCTTCAAAG GAGACAAAGG TTCTCAGGGC TCACCTGGAG CCATTGGATT TCCTGGACCC 
AGAGGTGACA AAGGAGAGGC TGGCCACCCC GGGCCACCAG GCTTGCCTGG AGCTCCTGGC 
TTCCCCAGCC TCATCAAGGG ACTTAGTGGG AGACCAGGCC CCCCTGGCTC CACAGGACTA 
CGGGGCTTAC CTGGCCTGAA GGGGTACCCT GGAATTGCAG GTTTCCAAGG AATTCCAGGA 
GAAAGTGGTT CACAGGGTCT CAGTGGAGTT CCCGGGCTAC CAGGAGCATC TGGTCTCCCA 
GGTTTAAAAG GAGATCAAGG CCAGACACTT GGAATTTCTG GTAGCCCAGG ACCCAAGGGA 
CAACCTGGGG AGTCTGGTTT CAAAGGTGTG AAAGGAAAAG ACGGACCAGT TGGTGATGTG 
GGTTTCCCAG GAAACAAAGG TGAAGATGGG AAAGTTGGTA TTTCTGGAGA TGTTGGCCTT 
CCTGGCTCCC TAGGACTCCC CGGAGTTGCA GGCATGAGAG GAAATCCAGG GCTTCCAGGT 
TCTCCAGGCC ACCCAGGGGC AACTGGGCCC CTGGGTCCCC CTGGCCTAAT AGGAACCAAA 
GGTTTCCCTG GACTTCCAGG TTTACATGGA CTGAATGGGC TTCCAGGAAC CAAGGGGACT 
CATGGAACTC CAGGACCTAG TATAACCGGT GTGCCTGGGC CAGCTGGCTT GCCTGGTCCC 
AAAGGGGAAA AAGGTTCTCC AGGAATTGGC ATCGGAGCCC CAGGAAAGCC AGGTATAAGA 
GGACCCAAAG GTAGTCAAGG TTTTCCAGGT CTCCAGGGCG CCGCTGGTCT CCCCGGTGCA 
CTGGGCCTCT CCTTGCCTTC AGTCATAGAA GGACAGCCTG GTGACCCTGG GCGACCAGGT 
CTAGATGGAG AACGAGGCCG CCCAGGCCCT CCTGGGCCCC CAGGCCCCCC TGGGCCTGCC 
TCAGATCAAG GCGACCCTGG AGACCCTGGC TTCCCTGGAA TTCCTGGCCC TCAAGGGCCC 
AAAGGAGACC AAGGAATTCC AGGTTTCTCT GGCCTCCCTG GAGAGCTAGG ATTGAAAGGC 
ATGAGAGGTG AGCCTGGCTT CATGGGGACT CCAGGCAAGG TTGGACCACC TGGAGACCCA 
GGACTTCCCG GGATGAAGGG GAAGGCGGGG CCAAGAGGCT TATCTGGCCC TCGAGGTGCT 
CCTGGACACA CACCCATTGC AGAAGCCATC CAGGCTCCCC CTGGACCCAT GGGTCTTCCA 
GGCATTGATG GAATCCCTGG CCTCATGGGA GACCCTGGGG TCCAAGGACC TGTGGGCCTA 
CAAGGATCTA AAGGTTTACC TGGCATCCCT GGCAAAGATG GCGCCAGTGG ACTCCCTGGC 
CCACCTGGGG CTCTTGGTGA TCCTGGTCTC CCTGGACTAC AAGGCCCTCC AGGATTTGAA 
GGAGCTCCAG GGAAGCCGGG CCCCTTCGGG AGGGCTGGAG TGCCTGGACA GAGCCTGAGA 
GTGGGCTACA CCTTGGTGAA GCACAGCCAG TCAGAACAGG TGCCCCTGTG CCCCAGCGGG 
ATGAGCCAGC TCTGGGTGGG TTACAGCCTG CTGTTCGTGG AGGGGCAAGA GAAAGCCCAC 
AACCAGGACC TGGGGTTTGC TGGCTCCTGC CTGCCCCGCT TCAGCACCAT GCCATTCATC 
TACTGCAACA TCAATGAAGT GTGCCACTAT GCCCGACGGA ATGATAAGTC CTACTGGCTC 
TCCACCACCG CCCCCATCCC TATGATGCCG GTCGGCCAGA CACAGATCCC CCAGTACATC 
AGCCGCTGCT CTGTGTGTGA GGCGCCCTCG CAAGCCATTG CTGTGCACAG CCAGGACATC 
ACCATCCCAC AGTGCCCCCT GGGCTGGCGC AGTCTGTGGA TTGGGTACTC CTTCCTCATG 
CACACAGCCG CCGGTGCAGA GGGCGGGGGC CAGTCCCTGG TCTCTCCCGG ATCCTGCCTA 
GAGGATTTCC GGGCTACTCC TTTCATCGAA TGCAGTGGTG CCCGGGGCAC CTGCCACTAC 
TTTGCCAACA AATATAGTTT CTGGCTGACA ACAGTGGAAG AGAGGCAGCA GTTTGGGGAG 
GAGCCTGTAT CTGAGACGCT GAAAGCTGGA CAGCTCCACA CCCGAGTCAG CCGCTGCCAG 
GTGTGTATGA AAAGCGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( XM_601826.8 ) cDNA ORF clone, Bos taurus(cattle) -> XP_601826.4 Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa12979
Clone ID Related Accession (Same CDS sequence)	XM_005227897.3 , XM_601826.8 , XM_005227897.4
Accession Version	XM_601826.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-6(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_601826.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT 
AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA 
ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC 
TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC 
GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA 
CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT 
GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA 
CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG 
TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA 
CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA 
TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT 
CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA 
GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG 
CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC 
CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC 
CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG 
GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT 
GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA 
GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA 
CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT 
TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA 
GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA 
CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA 
AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT 
GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA 
CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA 
CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA 
CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG 
CAACCAGGCC CCCGTGGAGT TCCAGGTGAC TGCTGCTGCA AGGAGGAGGT TGGGAAAACA 
GACTTAGACA CAGAGGGAGG TATCACCTTG CCTTGTATTA TTCCTGGACC GTATGGTCCA 
TCAGGACTCC CAGGAGCTCC CGGATTCCCA GGCCCTAAAG GGGCCCGTGG CCTCCCTGGG 
GCTCCAGGCC AACCTGGATT GCGTGGAAAT AAAGGGGACC CCGGAAGTCC AGGATTGGTT 
CACCTGCCTG AATTGCCAGG GTTTCCTGGA CCTCATGGAG AGAAGGGCTT ACCTGGGTTT 
CCTGGGCTCC CTGGAAAAGA CGGCTTGCCT GGGAAAGTTG GCAGCCCCGG CTTACCAGGT 
CCCAAGGGAG CCCCTGGTGA CATCCTTGGT GCTGAAAATG GTGCTCCGGG GGAGCAAGGC 
CTACAGGGAC TGCCTGGGGA CAGAGGATTT CCTGGAGACT CTGGCCTTCC AGGACTCAAG 
GGTTTGCTTG GGAAGTCGGG CTTGCTCGGC CCCAAAGGTG AGCGGGGCAG CCCTGGAATA 
CCAGGCCGTG TGGGTCAGCC AGGACCCCCA GGCCCTAGTG GTCTATTTGG CATCAAGGGC 
AAACCCGGGC TCCGCGGACC CCCAGGCTTT CCAGGCATTT CAGGATATCC TGGAAAGAAA 
GGTCCAAGAG GCGAGATAGG TCTCCCTGCG TCAGCTGGAA AGAGAGGCCT GCCTGGGCTG 
AAAGGGCTTC CGGGACTGCC AGGGCCAATT GGCTTCCTGG GGAACTCAGG CTTGCCAGGG 
GCCACTGGGT TACCAGGCCT GCCAGGTCTG AAGGGTGAGA AGGGGTCTGT TGGACTGACA 
GGTTTTCCAG GGATTCCAGG TCTCCCTGGT ATTCCTGGAG CAAGTGGATT AAAGGGAATT 
CCTGGGTCGA CAGGAAGAGT GGGGCCATCT GGATGGGCTG GTAGCCCTGG TGAAAAAGGA 
GACAGAGGTG ACCCAGGCCC CTTTGGGATA CCCAGTCATC GACCTCCAAT GCTGAACCTC 
CGCTTCAAAG GAGACAAAGG TTCTCAGGGC TCACCTGGAG CCATTGGATT TCCTGGACCC 
AGAGGTGACA AAGGAGAGGC TGGCCACCCC GGGCCACCAG GCTTGCCTGG AGCTCCTGGC 
TTCCCCAGCC TCATCAAGGG ACTTAGTGGG AGACCAGGCC CCCCTGGCTC CACAGGACTA 
CGGGGCTTAC CTGGCCTGAA GGGGTACCCT GGAATTGCAG GTTTCCAAGG AATTCCAGGA 
GAAAGTGGTT CACAGGGTCT CAGTGGAGTT CCCGGGCTAC CAGGAGCATC TGGTCTCCCA 
GGTTTAAAAG GAGATCAAGG CCAGACACTT GGAATTTCTG GTAGCCCAGG ACCCAAGGGA 
CAACCTGGGG AGTCTGGTTT CAAAGGTGTG AAAGGAAAAG ACGGACCAGT TGGTGATGTG 
GGTTTCCCAG GAAACAAAGG TGAAGATGGG AAAGTTGGTA TTTCTGGAGA TGTTGGCCTT 
CCTGGCTCCC TAGGACTCCC CGGAGTTGCA GGCATGAGAG GAAATCCAGG GCTTCCAGGT 
TCTCCAGGCC ACCCAGGGGC AACTGGGCCC CTGGGTCCCC CTGGCCTAAT AGGAACCAAA 
GGTTTCCCTG GACTTCCAGG TTTACATGGA CTGAATGGGC TTCCAGGAAC CAAGGGGACT 
CATGGAACTC CAGGACCTAG TATAACCGGT GTGCCTGGGC CAGCTGGCTT GCCTGGTCCC 
AAAGGGGAAA AAGGTTCTCC AGGAATTGGC ATCGGAGCCC CAGGAAAGCC AGGTATAAGA 
GGACCCAAAG GTAGTCAAGG TTTTCCAGGT CTCCAGGGCG CCGCTGGTCT CCCCGGTGCA 
CTGGGCCTCT CCTTGCCTTC AGTCATAGAA GGACAGCCTG GTGACCCTGG GCGACCAGGT 
CTAGATGGAG AACGAGGCCG CCCAGGCCCT CCTGGGCCCC CAGGCCCCCC TGGGCCTGCC 
TCAGATCAAG GCGACCCTGG AGACCCTGGC TTCCCTGGAA TTCCTGGCCC TCAAGGGCCC 
AAAGGAGACC AAGGAATTCC AGGTTTCTCT GGCCTCCCTG GAGAGCTAGG ATTGAAAGGC 
ATGAGAGGTG AGCCTGGCTT CATGGGGACT CCAGGCAAGG TTGGACCACC TGGAGACCCA 
GGACTTCCCG GGATGAAGGG GAAGGCGGGG CCAAGAGGCT TATCTGGCCC TCGAGGTGCT 
CCTGGACACA CACCCATTGC AGAAGCCATC CAGGCTCCCC CTGGACCCAT GGGTCTTCCA 
GGCATTGATG GAATCCCTGG CCTCATGGGA GACCCTGGGG TCCAAGGACC TGTGGGCCTA 
CAAGGATCTA AAGGTTTACC TGGCATCCCT GGCAAAGATG GCGCCAGTGG ACTCCCTGGC 
CCACCTGGGG CTCTTGGTGA TCCTGGTCTC CCTGGACTAC AAGGCCCTCC AGGATTTGAA 
GGAGCTCCAG GGAAGCCGGG CCCCTTCGGG AGGGCTGGAG TGCCTGGACA GAGCCTGAGA 
GTGGGCTACA CCTTGGTGAA GCACAGCCAG TCAGAACAGG TGCCCCTGTG CCCCAGCGGG 
ATGAGCCAGC TCTGGGTGGG TTACAGCCTG CTGTTCGTGG AGGGGCAAGA GAAAGCCCAC 
AACCAGGACC TGGGGTTTGC TGGCTCCTGC CTGCCCCGCT TCAGCACCAT GCCATTCATC 
TACTGCAACA TCAATGAAGT GTGCCACTAT GCCCGACGGA ATGATAAGTC CTACTGGCTC 
TCCACCACCG CCCCCATCCC TATGATGCCG GTCGGCCAGA CACAGATCCC CCAGTACATC 
AGCCGCTGCT CTGTGTGTGA GGCGCCCTCG CAAGCCATTG CTGTGCACAG CCAGGACATC 
ACCATCCCAC AGTGCCCCCT GGGCTGGCGC AGTCTGTGGA TTGGGTACTC CTTCCTCATG 
CACACAGCCG CCGGTGCAGA GGGCGGGGGC CAGTCCCTGG TCTCTCCCGG ATCCTGCCTA 
GAGGATTTCC GGGCTACTCC TTTCATCGAA TGCAGTGGTG CCCGGGGCAC CTGCCACTAC 
TTTGCCAACA AATATAGTTT CTGGCTGACA ACAGTGGAAG AGAGGCAGCA GTTTGGGGAG 
GAGCCTGTAT CTGAGACGCT GAAAGCTGGA CAGCTCCACA CCCGAGTCAG CCGCTGCCAG 
GTGTGTATGA AAAGCGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_601826.4
CDS	270..5390
Translation	MHPGLWLLLVTLCLTEELAGAGEKKPYGKPCGGQDCSGGCKCSPEKGARGRPGPIGIQGPTGPQGLPGPSGLSGLKGERGSPGPLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYSGLPGPPGLPGQKGSKGEPVLAQGSFKGMKGESGLPGLDGITGPQGAPGSPGAVGPRGPPGLQGPPGPPGFPGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPQGFPGVSGPPGLPGFGIAGEKGEKGIPGLPGPRGPMGLEGVQGPPGSQGRKGSSGFPGLNGFPGIKGEKGGIGLPGPDIFIDTDGAVISGYPGDPGMPGLPGLKGDEGIQGLPGPPGAPGLPALPGGPGALGPQGALGLKGERGNTGRTTTGAAGLPGRDGLPGLPGLPGPPGAASETGTLQSTEPGSPGLRGERGPKGHPGLKGIKGDPGFCACDGGVPNMGPPGEPGLPGPPGLLGLPGLKGTKGDPGSGGAQGPSGDPGVFGPRGPTGPKGKKGDPTLSTRSGMPGEQGDPGPQGLPGETGAPGKDGIPGLPGLPGLLGDGAQGFPGEKGLPGLPGEKGHTGPTGPPGIGLPGAPGPRGLPGDKGVDGLPGQPGPRGVPGDCCCKEEVGKTDLDTEGGITLPCIIPGPYGPSGLPGAPGFPGPKGARGLPGAPGQPGLRGNKGDPGSPGLVHLPELPGFPGPHGEKGLPGFPGLPGKDGLPGKVGSPGLPGPKGAPGDILGAENGAPGEQGLQGLPGDRGFPGDSGLPGLKGLLGKSGLLGPKGERGSPGIPGRVGQPGPPGPSGLFGIKGKPGLRGPPGFPGISGYPGKKGPRGEIGLPASAGKRGLPGLKGLPGLPGPIGFLGNSGLPGATGLPGLPGLKGEKGSVGLTGFPGIPGLPGIPGASGLKGIPGSTGRVGPSGWAGSPGEKGDRGDPGPFGIPSHRPPMLNLRFKGDKGSQGSPGAIGFPGPRGDKGEAGHPGPPGLPGAPGFPSLIKGLSGRPGPPGSTGLRGLPGLKGYPGIAGFQGIPGESGSQGLSGVPGLPGASGLPGLKGDQGQTLGISGSPGPKGQPGESGFKGVKGKDGPVGDVGFPGNKGEDGKVGISGDVGLPGSLGLPGVAGMRGNPGLPGSPGHPGATGPLGPPGLIGTKGFPGLPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGSPGIGIGAPGKPGIRGPKGSQGFPGLQGAAGLPGALGLSLPSVIEGQPGDPGRPGLDGERGRPGPPGPPGPPGPASDQGDPGDPGFPGIPGPQGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGLPGMKGKAGPRGLSGPRGAPGHTPIAEAIQAPPGPMGLPGIDGIPGLMGDPGVQGPVGLQGSKGLPGIPGKDGASGLPGPPGALGDPGLPGLQGPPGFEGAPGKPGPFGRAGVPGQSLRVGYTLVKHSQSEQVPLCPSGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVGQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGEEPVSETLKAGQLHTRVSRCQVCMKSV

Target ORF information:

RefSeq Version	XM_601826.8
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_601826.8

ORF Insert Sequence:

ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT 
AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA 
ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC 
TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC 
GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA 
CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT 
GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA 
CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG 
TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA 
CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA 
TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT 
CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA 
GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG 
CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC 
CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC 
CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG 
GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT 
GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA 
GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA 
CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT 
TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA 
GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA 
CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA 
AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT 
GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA 
CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA 
CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA 
CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG 
CAACCAGGCC CCCGTGGAGT TCCAGGTGAC TGCTGCTGCA AGGAGGAGGT TGGGAAAACA 
GACTTAGACA CAGAGGGAGG TATCACCTTG CCTTGTATTA TTCCTGGACC GTATGGTCCA 
TCAGGACTCC CAGGAGCTCC CGGATTCCCA GGCCCTAAAG GGGCCCGTGG CCTCCCTGGG 
GCTCCAGGCC AACCTGGATT GCGTGGAAAT AAAGGGGACC CCGGAAGTCC AGGATTGGTT 
CACCTGCCTG AATTGCCAGG GTTTCCTGGA CCTCATGGAG AGAAGGGCTT ACCTGGGTTT 
CCTGGGCTCC CTGGAAAAGA CGGCTTGCCT GGGAAAGTTG GCAGCCCCGG CTTACCAGGT 
CCCAAGGGAG CCCCTGGTGA CATCCTTGGT GCTGAAAATG GTGCTCCGGG GGAGCAAGGC 
CTACAGGGAC TGCCTGGGGA CAGAGGATTT CCTGGAGACT CTGGCCTTCC AGGACTCAAG 
GGTTTGCTTG GGAAGTCGGG CTTGCTCGGC CCCAAAGGTG AGCGGGGCAG CCCTGGAATA 
CCAGGCCGTG TGGGTCAGCC AGGACCCCCA GGCCCTAGTG GTCTATTTGG CATCAAGGGC 
AAACCCGGGC TCCGCGGACC CCCAGGCTTT CCAGGCATTT CAGGATATCC TGGAAAGAAA 
GGTCCAAGAG GCGAGATAGG TCTCCCTGCG TCAGCTGGAA AGAGAGGCCT GCCTGGGCTG 
AAAGGGCTTC CGGGACTGCC AGGGCCAATT GGCTTCCTGG GGAACTCAGG CTTGCCAGGG 
GCCACTGGGT TACCAGGCCT GCCAGGTCTG AAGGGTGAGA AGGGGTCTGT TGGACTGACA 
GGTTTTCCAG GGATTCCAGG TCTCCCTGGT ATTCCTGGAG CAAGTGGATT AAAGGGAATT 
CCTGGGTCGA CAGGAAGAGT GGGGCCATCT GGATGGGCTG GTAGCCCTGG TGAAAAAGGA 
GACAGAGGTG ACCCAGGCCC CTTTGGGATA CCCAGTCATC GACCTCCAAT GCTGAACCTC 
CGCTTCAAAG GAGACAAAGG TTCTCAGGGC TCACCTGGAG CCATTGGATT TCCTGGACCC 
AGAGGTGACA AAGGAGAGGC TGGCCACCCC GGGCCACCAG GCTTGCCTGG AGCTCCTGGC 
TTCCCCAGCC TCATCAAGGG ACTTAGTGGG AGACCAGGCC CCCCTGGCTC CACAGGACTA 
CGGGGCTTAC CTGGCCTGAA GGGGTACCCT GGAATTGCAG GTTTCCAAGG AATTCCAGGA 
GAAAGTGGTT CACAGGGTCT CAGTGGAGTT CCCGGGCTAC CAGGAGCATC TGGTCTCCCA 
GGTTTAAAAG GAGATCAAGG CCAGACACTT GGAATTTCTG GTAGCCCAGG ACCCAAGGGA 
CAACCTGGGG AGTCTGGTTT CAAAGGTGTG AAAGGAAAAG ACGGACCAGT TGGTGATGTG 
GGTTTCCCAG GAAACAAAGG TGAAGATGGG AAAGTTGGTA TTTCTGGAGA TGTTGGCCTT 
CCTGGCTCCC TAGGACTCCC CGGAGTTGCA GGCATGAGAG GAAATCCAGG GCTTCCAGGT 
TCTCCAGGCC ACCCAGGGGC AACTGGGCCC CTGGGTCCCC CTGGCCTAAT AGGAACCAAA 
GGTTTCCCTG GACTTCCAGG TTTACATGGA CTGAATGGGC TTCCAGGAAC CAAGGGGACT 
CATGGAACTC CAGGACCTAG TATAACCGGT GTGCCTGGGC CAGCTGGCTT GCCTGGTCCC 
AAAGGGGAAA AAGGTTCTCC AGGAATTGGC ATCGGAGCCC CAGGAAAGCC AGGTATAAGA 
GGACCCAAAG GTAGTCAAGG TTTTCCAGGT CTCCAGGGCG CCGCTGGTCT CCCCGGTGCA 
CTGGGCCTCT CCTTGCCTTC AGTCATAGAA GGACAGCCTG GTGACCCTGG GCGACCAGGT 
CTAGATGGAG AACGAGGCCG CCCAGGCCCT CCTGGGCCCC CAGGCCCCCC TGGGCCTGCC 
TCAGATCAAG GCGACCCTGG AGACCCTGGC TTCCCTGGAA TTCCTGGCCC TCAAGGGCCC 
AAAGGAGACC AAGGAATTCC AGGTTTCTCT GGCCTCCCTG GAGAGCTAGG ATTGAAAGGC 
ATGAGAGGTG AGCCTGGCTT CATGGGGACT CCAGGCAAGG TTGGACCACC TGGAGACCCA 
GGACTTCCCG GGATGAAGGG GAAGGCGGGG CCAAGAGGCT TATCTGGCCC TCGAGGTGCT 
CCTGGACACA CACCCATTGC AGAAGCCATC CAGGCTCCCC CTGGACCCAT GGGTCTTCCA 
GGCATTGATG GAATCCCTGG CCTCATGGGA GACCCTGGGG TCCAAGGACC TGTGGGCCTA 
CAAGGATCTA AAGGTTTACC TGGCATCCCT GGCAAAGATG GCGCCAGTGG ACTCCCTGGC 
CCACCTGGGG CTCTTGGTGA TCCTGGTCTC CCTGGACTAC AAGGCCCTCC AGGATTTGAA 
GGAGCTCCAG GGAAGCCGGG CCCCTTCGGG AGGGCTGGAG TGCCTGGACA GAGCCTGAGA 
GTGGGCTACA CCTTGGTGAA GCACAGCCAG TCAGAACAGG TGCCCCTGTG CCCCAGCGGG 
ATGAGCCAGC TCTGGGTGGG TTACAGCCTG CTGTTCGTGG AGGGGCAAGA GAAAGCCCAC 
AACCAGGACC TGGGGTTTGC TGGCTCCTGC CTGCCCCGCT TCAGCACCAT GCCATTCATC 
TACTGCAACA TCAATGAAGT GTGCCACTAT GCCCGACGGA ATGATAAGTC CTACTGGCTC 
TCCACCACCG CCCCCATCCC TATGATGCCG GTCGGCCAGA CACAGATCCC CCAGTACATC 
AGCCGCTGCT CTGTGTGTGA GGCGCCCTCG CAAGCCATTG CTGTGCACAG CCAGGACATC 
ACCATCCCAC AGTGCCCCCT GGGCTGGCGC AGTCTGTGGA TTGGGTACTC CTTCCTCATG 
CACACAGCCG CCGGTGCAGA GGGCGGGGGC CAGTCCCTGG TCTCTCCCGG ATCCTGCCTA 
GAGGATTTCC GGGCTACTCC TTTCATCGAA TGCAGTGGTG CCCGGGGCAC CTGCCACTAC 
TTTGCCAACA AATATAGTTT CTGGCTGACA ACAGTGGAAG AGAGGCAGCA GTTTGGGGAG 
GAGCCTGTAT CTGAGACGCT GAAAGCTGGA CAGCTCCACA CCCGAGTCAG CCGCTGCCAG 
GTGTGTATGA AAAGCGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( XM_005227897.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227954.1 Bos taurus collagen type IV alpha 6 chain (COL4A6), transcript variant X1, mRNA.

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CloneID	OBa12979
Clone ID Related Accession (Same CDS sequence)	XM_005227897.3 , XM_601826.8 , XM_005227897.4
Accession Version	XM_005227897.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-6(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227897.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT 
AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA 
ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC 
TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC 
GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA 
CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT 
GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA 
CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG 
TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA 
CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA 
TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT 
CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA 
GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG 
CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC 
CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC 
CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG 
GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT 
GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA 
GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA 
CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT 
TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA 
GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA 
CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA 
AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT 
GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA 
CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA 
CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA 
CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG 
CAACCAGGCC CCCGTGGAGT TCCAGGTGAC TGCTGCTGCA AGGAGGAGGT TGGGAAAACA 
GACTTAGACA CAGAGGGAGG TATCACCTTG CCTTGTATTA TTCCTGGACC GTATGGTCCA 
TCAGGACTCC CAGGAGCTCC CGGATTCCCA GGCCCTAAAG GGGCCCGTGG CCTCCCTGGG 
GCTCCAGGCC AACCTGGATT GCGTGGAAAT AAAGGGGACC CCGGAAGTCC AGGATTGGTT 
CACCTGCCTG AATTGCCAGG GTTTCCTGGA CCTCATGGAG AGAAGGGCTT ACCTGGGTTT 
CCTGGGCTCC CTGGAAAAGA CGGCTTGCCT GGGAAAGTTG GCAGCCCCGG CTTACCAGGT 
CCCAAGGGAG CCCCTGGTGA CATCCTTGGT GCTGAAAATG GTGCTCCGGG GGAGCAAGGC 
CTACAGGGAC TGCCTGGGGA CAGAGGATTT CCTGGAGACT CTGGCCTTCC AGGACTCAAG 
GGTTTGCTTG GGAAGTCGGG CTTGCTCGGC CCCAAAGGTG AGCGGGGCAG CCCTGGAATA 
CCAGGCCGTG TGGGTCAGCC AGGACCCCCA GGCCCTAGTG GTCTATTTGG CATCAAGGGC 
AAACCCGGGC TCCGCGGACC CCCAGGCTTT CCAGGCATTT CAGGATATCC TGGAAAGAAA 
GGTCCAAGAG GCGAGATAGG TCTCCCTGCG TCAGCTGGAA AGAGAGGCCT GCCTGGGCTG 
AAAGGGCTTC CGGGACTGCC AGGGCCAATT GGCTTCCTGG GGAACTCAGG CTTGCCAGGG 
GCCACTGGGT TACCAGGCCT GCCAGGTCTG AAGGGTGAGA AGGGGTCTGT TGGACTGACA 
GGTTTTCCAG GGATTCCAGG TCTCCCTGGT ATTCCTGGAG CAAGTGGATT AAAGGGAATT 
CCTGGGTCGA CAGGAAGAGT GGGGCCATCT GGATGGGCTG GTAGCCCTGG TGAAAAAGGA 
GACAGAGGTG ACCCAGGCCC CTTTGGGATA CCCAGTCATC GACCTCCAAT GCTGAACCTC 
CGCTTCAAAG GAGACAAAGG TTCTCAGGGC TCACCTGGAG CCATTGGATT TCCTGGACCC 
AGAGGTGACA AAGGAGAGGC TGGCCACCCC GGGCCACCAG GCTTGCCTGG AGCTCCTGGC 
TTCCCCAGCC TCATCAAGGG ACTTAGTGGG AGACCAGGCC CCCCTGGCTC CACAGGACTA 
CGGGGCTTAC CTGGCCTGAA GGGGTACCCT GGAATTGCAG GTTTCCAAGG AATTCCAGGA 
GAAAGTGGTT CACAGGGTCT CAGTGGAGTT CCCGGGCTAC CAGGAGCATC TGGTCTCCCA 
GGTTTAAAAG GAGATCAAGG CCAGACACTT GGAATTTCTG GTAGCCCAGG ACCCAAGGGA 
CAACCTGGGG AGTCTGGTTT CAAAGGTGTG AAAGGAAAAG ACGGACCAGT TGGTGATGTG 
GGTTTCCCAG GAAACAAAGG TGAAGATGGG AAAGTTGGTA TTTCTGGAGA TGTTGGCCTT 
CCTGGCTCCC TAGGACTCCC CGGAGTTGCA GGCATGAGAG GAAATCCAGG GCTTCCAGGT 
TCTCCAGGCC ACCCAGGGGC AACTGGGCCC CTGGGTCCCC CTGGCCTAAT AGGAACCAAA 
GGTTTCCCTG GACTTCCAGG TTTACATGGA CTGAATGGGC TTCCAGGAAC CAAGGGGACT 
CATGGAACTC CAGGACCTAG TATAACCGGT GTGCCTGGGC CAGCTGGCTT GCCTGGTCCC 
AAAGGGGAAA AAGGTTCTCC AGGAATTGGC ATCGGAGCCC CAGGAAAGCC AGGTATAAGA 
GGACCCAAAG GTAGTCAAGG TTTTCCAGGT CTCCAGGGCG CCGCTGGTCT CCCCGGTGCA 
CTGGGCCTCT CCTTGCCTTC AGTCATAGAA GGACAGCCTG GTGACCCTGG GCGACCAGGT 
CTAGATGGAG AACGAGGCCG CCCAGGCCCT CCTGGGCCCC CAGGCCCCCC TGGGCCTGCC 
TCAGATCAAG GCGACCCTGG AGACCCTGGC TTCCCTGGAA TTCCTGGCCC TCAAGGGCCC 
AAAGGAGACC AAGGAATTCC AGGTTTCTCT GGCCTCCCTG GAGAGCTAGG ATTGAAAGGC 
ATGAGAGGTG AGCCTGGCTT CATGGGGACT CCAGGCAAGG TTGGACCACC TGGAGACCCA 
GGACTTCCCG GGATGAAGGG GAAGGCGGGG CCAAGAGGCT TATCTGGCCC TCGAGGTGCT 
CCTGGACACA CACCCATTGC AGAAGCCATC CAGGCTCCCC CTGGACCCAT GGGTCTTCCA 
GGCATTGATG GAATCCCTGG CCTCATGGGA GACCCTGGGG TCCAAGGACC TGTGGGCCTA 
CAAGGATCTA AAGGTTTACC TGGCATCCCT GGCAAAGATG GCGCCAGTGG ACTCCCTGGC 
CCACCTGGGG CTCTTGGTGA TCCTGGTCTC CCTGGACTAC AAGGCCCTCC AGGATTTGAA 
GGAGCTCCAG GGAAGCCGGG CCCCTTCGGG AGGGCTGGAG TGCCTGGACA GAGCCTGAGA 
GTGGGCTACA CCTTGGTGAA GCACAGCCAG TCAGAACAGG TGCCCCTGTG CCCCAGCGGG 
ATGAGCCAGC TCTGGGTGGG TTACAGCCTG CTGTTCGTGG AGGGGCAAGA GAAAGCCCAC 
AACCAGGACC TGGGGTTTGC TGGCTCCTGC CTGCCCCGCT TCAGCACCAT GCCATTCATC 
TACTGCAACA TCAATGAAGT GTGCCACTAT GCCCGACGGA ATGATAAGTC CTACTGGCTC 
TCCACCACCG CCCCCATCCC TATGATGCCG GTCGGCCAGA CACAGATCCC CCAGTACATC 
AGCCGCTGCT CTGTGTGTGA GGCGCCCTCG CAAGCCATTG CTGTGCACAG CCAGGACATC 
ACCATCCCAC AGTGCCCCCT GGGCTGGCGC AGTCTGTGGA TTGGGTACTC CTTCCTCATG 
CACACAGCCG CCGGTGCAGA GGGCGGGGGC CAGTCCCTGG TCTCTCCCGG ATCCTGCCTA 
GAGGATTTCC GGGCTACTCC TTTCATCGAA TGCAGTGGTG CCCGGGGCAC CTGCCACTAC 
TTTGCCAACA AATATAGTTT CTGGCTGACA ACAGTGGAAG AGAGGCAGCA GTTTGGGGAG 
GAGCCTGTAT CTGAGACGCT GAAAGCTGGA CAGCTCCACA CCCGAGTCAG CCGCTGCCAG 
GTGTGTATGA AAAGCGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227954.1
CDS	223..5343
Translation	MHPGLWLLLVTLCLTEELAGAGEKKPYGKPCGGQDCSGGCKCSPEKGARGRPGPIGIQGPTGPQGLPGPSGLSGLKGERGSPGPLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYSGLPGPPGLPGQKGSKGEPVLAQGSFKGMKGESGLPGLDGITGPQGAPGSPGAVGPRGPPGLQGPPGPPGFPGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPQGFPGVSGPPGLPGFGIAGEKGEKGIPGLPGPRGPMGLEGVQGPPGSQGRKGSSGFPGLNGFPGIKGEKGGIGLPGPDIFIDTDGAVISGYPGDPGMPGLPGLKGDEGIQGLPGPPGAPGLPALPGGPGALGPQGALGLKGERGNTGRTTTGAAGLPGRDGLPGLPGLPGPPGAASETGTLQSTEPGSPGLRGERGPKGHPGLKGIKGDPGFCACDGGVPNMGPPGEPGLPGPPGLLGLPGLKGTKGDPGSGGAQGPSGDPGVFGPRGPTGPKGKKGDPTLSTRSGMPGEQGDPGPQGLPGETGAPGKDGIPGLPGLPGLLGDGAQGFPGEKGLPGLPGEKGHTGPTGPPGIGLPGAPGPRGLPGDKGVDGLPGQPGPRGVPGDCCCKEEVGKTDLDTEGGITLPCIIPGPYGPSGLPGAPGFPGPKGARGLPGAPGQPGLRGNKGDPGSPGLVHLPELPGFPGPHGEKGLPGFPGLPGKDGLPGKVGSPGLPGPKGAPGDILGAENGAPGEQGLQGLPGDRGFPGDSGLPGLKGLLGKSGLLGPKGERGSPGIPGRVGQPGPPGPSGLFGIKGKPGLRGPPGFPGISGYPGKKGPRGEIGLPASAGKRGLPGLKGLPGLPGPIGFLGNSGLPGATGLPGLPGLKGEKGSVGLTGFPGIPGLPGIPGASGLKGIPGSTGRVGPSGWAGSPGEKGDRGDPGPFGIPSHRPPMLNLRFKGDKGSQGSPGAIGFPGPRGDKGEAGHPGPPGLPGAPGFPSLIKGLSGRPGPPGSTGLRGLPGLKGYPGIAGFQGIPGESGSQGLSGVPGLPGASGLPGLKGDQGQTLGISGSPGPKGQPGESGFKGVKGKDGPVGDVGFPGNKGEDGKVGISGDVGLPGSLGLPGVAGMRGNPGLPGSPGHPGATGPLGPPGLIGTKGFPGLPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGSPGIGIGAPGKPGIRGPKGSQGFPGLQGAAGLPGALGLSLPSVIEGQPGDPGRPGLDGERGRPGPPGPPGPPGPASDQGDPGDPGFPGIPGPQGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGLPGMKGKAGPRGLSGPRGAPGHTPIAEAIQAPPGPMGLPGIDGIPGLMGDPGVQGPVGLQGSKGLPGIPGKDGASGLPGPPGALGDPGLPGLQGPPGFEGAPGKPGPFGRAGVPGQSLRVGYTLVKHSQSEQVPLCPSGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVGQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGEEPVSETLKAGQLHTRVSRCQVCMKSV

Target ORF information:

RefSeq Version	XM_005227897.4
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type IV alpha 6 chain (COL4A6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227897.4

ORF Insert Sequence:

ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT 
AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA 
ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC 
TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC 
GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA 
CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT 
GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA 
CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG 
TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA 
CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA 
TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT 
CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA 
GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG 
CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC 
CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC 
CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG 
GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT 
GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA 
GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA 
CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT 
TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA 
GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA 
CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA 
AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT 
GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA 
CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA 
CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA 
CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG 
CAACCAGGCC CCCGTGGAGT TCCAGGTGAC TGCTGCTGCA AGGAGGAGGT TGGGAAAACA 
GACTTAGACA CAGAGGGAGG TATCACCTTG CCTTGTATTA TTCCTGGACC GTATGGTCCA 
TCAGGACTCC CAGGAGCTCC CGGATTCCCA GGCCCTAAAG GGGCCCGTGG CCTCCCTGGG 
GCTCCAGGCC AACCTGGATT GCGTGGAAAT AAAGGGGACC CCGGAAGTCC AGGATTGGTT 
CACCTGCCTG AATTGCCAGG GTTTCCTGGA CCTCATGGAG AGAAGGGCTT ACCTGGGTTT 
CCTGGGCTCC CTGGAAAAGA CGGCTTGCCT GGGAAAGTTG GCAGCCCCGG CTTACCAGGT 
CCCAAGGGAG CCCCTGGTGA CATCCTTGGT GCTGAAAATG GTGCTCCGGG GGAGCAAGGC 
CTACAGGGAC TGCCTGGGGA CAGAGGATTT CCTGGAGACT CTGGCCTTCC AGGACTCAAG 
GGTTTGCTTG GGAAGTCGGG CTTGCTCGGC CCCAAAGGTG AGCGGGGCAG CCCTGGAATA 
CCAGGCCGTG TGGGTCAGCC AGGACCCCCA GGCCCTAGTG GTCTATTTGG CATCAAGGGC 
AAACCCGGGC TCCGCGGACC CCCAGGCTTT CCAGGCATTT CAGGATATCC TGGAAAGAAA 
GGTCCAAGAG GCGAGATAGG TCTCCCTGCG TCAGCTGGAA AGAGAGGCCT GCCTGGGCTG 
AAAGGGCTTC CGGGACTGCC AGGGCCAATT GGCTTCCTGG GGAACTCAGG CTTGCCAGGG 
GCCACTGGGT TACCAGGCCT GCCAGGTCTG AAGGGTGAGA AGGGGTCTGT TGGACTGACA 
GGTTTTCCAG GGATTCCAGG TCTCCCTGGT ATTCCTGGAG CAAGTGGATT AAAGGGAATT 
CCTGGGTCGA CAGGAAGAGT GGGGCCATCT GGATGGGCTG GTAGCCCTGG TGAAAAAGGA 
GACAGAGGTG ACCCAGGCCC CTTTGGGATA CCCAGTCATC GACCTCCAAT GCTGAACCTC 
CGCTTCAAAG GAGACAAAGG TTCTCAGGGC TCACCTGGAG CCATTGGATT TCCTGGACCC 
AGAGGTGACA AAGGAGAGGC TGGCCACCCC GGGCCACCAG GCTTGCCTGG AGCTCCTGGC 
TTCCCCAGCC TCATCAAGGG ACTTAGTGGG AGACCAGGCC CCCCTGGCTC CACAGGACTA 
CGGGGCTTAC CTGGCCTGAA GGGGTACCCT GGAATTGCAG GTTTCCAAGG AATTCCAGGA 
GAAAGTGGTT CACAGGGTCT CAGTGGAGTT CCCGGGCTAC CAGGAGCATC TGGTCTCCCA 
GGTTTAAAAG GAGATCAAGG CCAGACACTT GGAATTTCTG GTAGCCCAGG ACCCAAGGGA 
CAACCTGGGG AGTCTGGTTT CAAAGGTGTG AAAGGAAAAG ACGGACCAGT TGGTGATGTG 
GGTTTCCCAG GAAACAAAGG TGAAGATGGG AAAGTTGGTA TTTCTGGAGA TGTTGGCCTT 
CCTGGCTCCC TAGGACTCCC CGGAGTTGCA GGCATGAGAG GAAATCCAGG GCTTCCAGGT 
TCTCCAGGCC ACCCAGGGGC AACTGGGCCC CTGGGTCCCC CTGGCCTAAT AGGAACCAAA 
GGTTTCCCTG GACTTCCAGG TTTACATGGA CTGAATGGGC TTCCAGGAAC CAAGGGGACT 
CATGGAACTC CAGGACCTAG TATAACCGGT GTGCCTGGGC CAGCTGGCTT GCCTGGTCCC 
AAAGGGGAAA AAGGTTCTCC AGGAATTGGC ATCGGAGCCC CAGGAAAGCC AGGTATAAGA 
GGACCCAAAG GTAGTCAAGG TTTTCCAGGT CTCCAGGGCG CCGCTGGTCT CCCCGGTGCA 
CTGGGCCTCT CCTTGCCTTC AGTCATAGAA GGACAGCCTG GTGACCCTGG GCGACCAGGT 
CTAGATGGAG AACGAGGCCG CCCAGGCCCT CCTGGGCCCC CAGGCCCCCC TGGGCCTGCC 
TCAGATCAAG GCGACCCTGG AGACCCTGGC TTCCCTGGAA TTCCTGGCCC TCAAGGGCCC 
AAAGGAGACC AAGGAATTCC AGGTTTCTCT GGCCTCCCTG GAGAGCTAGG ATTGAAAGGC 
ATGAGAGGTG AGCCTGGCTT CATGGGGACT CCAGGCAAGG TTGGACCACC TGGAGACCCA 
GGACTTCCCG GGATGAAGGG GAAGGCGGGG CCAAGAGGCT TATCTGGCCC TCGAGGTGCT 
CCTGGACACA CACCCATTGC AGAAGCCATC CAGGCTCCCC CTGGACCCAT GGGTCTTCCA 
GGCATTGATG GAATCCCTGG CCTCATGGGA GACCCTGGGG TCCAAGGACC TGTGGGCCTA 
CAAGGATCTA AAGGTTTACC TGGCATCCCT GGCAAAGATG GCGCCAGTGG ACTCCCTGGC 
CCACCTGGGG CTCTTGGTGA TCCTGGTCTC CCTGGACTAC AAGGCCCTCC AGGATTTGAA 
GGAGCTCCAG GGAAGCCGGG CCCCTTCGGG AGGGCTGGAG TGCCTGGACA GAGCCTGAGA 
GTGGGCTACA CCTTGGTGAA GCACAGCCAG TCAGAACAGG TGCCCCTGTG CCCCAGCGGG 
ATGAGCCAGC TCTGGGTGGG TTACAGCCTG CTGTTCGTGG AGGGGCAAGA GAAAGCCCAC 
AACCAGGACC TGGGGTTTGC TGGCTCCTGC CTGCCCCGCT TCAGCACCAT GCCATTCATC 
TACTGCAACA TCAATGAAGT GTGCCACTAT GCCCGACGGA ATGATAAGTC CTACTGGCTC 
TCCACCACCG CCCCCATCCC TATGATGCCG GTCGGCCAGA CACAGATCCC CCAGTACATC 
AGCCGCTGCT CTGTGTGTGA GGCGCCCTCG CAAGCCATTG CTGTGCACAG CCAGGACATC 
ACCATCCCAC AGTGCCCCCT GGGCTGGCGC AGTCTGTGGA TTGGGTACTC CTTCCTCATG 
CACACAGCCG CCGGTGCAGA GGGCGGGGGC CAGTCCCTGG TCTCTCCCGG ATCCTGCCTA 
GAGGATTTCC GGGCTACTCC TTTCATCGAA TGCAGTGGTG CCCGGGGCAC CTGCCACTAC 
TTTGCCAACA AATATAGTTT CTGGCTGACA ACAGTGGAAG AGAGGCAGCA GTTTGGGGAG 
GAGCCTGTAT CTGAGACGCT GAAAGCTGGA CAGCTCCACA CCCGAGTCAG CCGCTGCCAG 
GTGTGTATGA AAAGCGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( XM_010800459.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010798761.1 Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X2, mRNA.

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CloneID	OBa35386
Clone ID Related Accession (Same CDS sequence)	XM_010800459.2 , XM_002699886.5 , XM_002699886.4
Accession Version	XM_010800459.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-6(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010800459.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT 
AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA 
ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC 
TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC 
GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA 
CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT 
GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA 
CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG 
TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA 
CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA 
TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT 
CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA 
GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG 
CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC 
CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC 
CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG 
GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT 
GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA 
GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA 
CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT 
TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA 
GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA 
CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA 
AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT 
GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA 
CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA 
CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA 
CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG 
CAACCAGGCC CCCGTGGAGT TCCAGGTATC ACCTTGCCTT GTATTATTCC TGGACCGTAT 
GGTCCATCAG GACTCCCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC 
CCTGGGGCTC CAGGCCAACC TGGATTGCGT GGAAATAAAG GGGACCCCGG AAGTCCAGGA 
TTGGTTCACC TGCCTGAATT GCCAGGGTTT CCTGGACCTC ATGGAGAGAA GGGCTTACCT 
GGGTTTCCTG GGCTCCCTGG AAAAGACGGC TTGCCTGGGA AAGTTGGCAG CCCCGGCTTA 
CCAGGTCCCA AGGGAGCCCC TGGTGACATC CTTGGTGCTG AAAATGGTGC TCCGGGGGAG 
CAAGGCCTAC AGGGACTGCC TGGGGACAGA GGATTTCCTG GAGACTCTGG CCTTCCAGGA 
CTCAAGGGTT TGCTTGGGAA GTCGGGCTTG CTCGGCCCCA AAGGTGAGCG GGGCAGCCCT 
GGAATACCAG GCCGTGTGGG TCAGCCAGGA CCCCCAGGCC CTAGTGGTCT ATTTGGCATC 
AAGGGCAAAC CCGGGCTCCG CGGACCCCCA GGCTTTCCAG GCATTTCAGG ATATCCTGGA 
AAGAAAGGTC CAAGAGGCGA GATAGGTCTC CCTGCGTCAG CTGGAAAGAG AGGCCTGCCT 
GGGCTGAAAG GGCTTCCGGG ACTGCCAGGG CCAATTGGCT TCCTGGGGAA CTCAGGCTTG 
CCAGGGGCCA CTGGGTTACC AGGCCTGCCA GGTCTGAAGG GTGAGAAGGG GTCTGTTGGA 
CTGACAGGTT TTCCAGGGAT TCCAGGTCTC CCTGGTATTC CTGGAGCAAG TGGATTAAAG 
GGAATTCCTG GGTCGACAGG AAGAGTGGGG CCATCTGGAT GGGCTGGTAG CCCTGGTGAA 
AAAGGAGACA GAGGTGACCC AGGCCCCTTT GGGATACCCA GTCATCGACC TCCAATGCTG 
AACCTCCGCT TCAAAGGAGA CAAAGGTTCT CAGGGCTCAC CTGGAGCCAT TGGATTTCCT 
GGACCCAGAG GTGACAAAGG AGAGGCTGGC CACCCCGGGC CACCAGGCTT GCCTGGAGCT 
CCTGGCTTCC CCAGCCTCAT CAAGGGACTT AGTGGGAGAC CAGGCCCCCC TGGCTCCACA 
GGACTACGGG GCTTACCTGG CCTGAAGGGG TACCCTGGAA TTGCAGGTTT CCAAGGAATT 
CCAGGAGAAA GTGGTTCACA GGGTCTCAGT GGAGTTCCCG GGCTACCAGG AGCATCTGGT 
CTCCCAGGTT TAAAAGGAGA TCAAGGCCAG ACACTTGGAA TTTCTGGTAG CCCAGGACCC 
AAGGGACAAC CTGGGGAGTC TGGTTTCAAA GGTGTGAAAG GAAAAGACGG ACCAGTTGGT 
GATGTGGGTT TCCCAGGAAA CAAAGGTGAA GATGGGAAAG TTGGTATTTC TGGAGATGTT 
GGCCTTCCTG GCTCCCTAGG ACTCCCCGGA GTTGCAGGCA TGAGAGGAAA TCCAGGGCTT 
CCAGGTTCTC CAGGCCACCC AGGGGCAACT GGGCCCCTGG GTCCCCCTGG CCTAATAGGA 
ACCAAAGGTT TCCCTGGACT TCCAGGTTTA CATGGACTGA ATGGGCTTCC AGGAACCAAG 
GGGACTCATG GAACTCCAGG ACCTAGTATA ACCGGTGTGC CTGGGCCAGC TGGCTTGCCT 
GGTCCCAAAG GGGAAAAAGG TTCTCCAGGA ATTGGCATCG GAGCCCCAGG AAAGCCAGGT 
ATAAGAGGAC CCAAAGGTAG TCAAGGTTTT CCAGGTCTCC AGGGCGCCGC TGGTCTCCCC 
GGTGCACTGG GCCTCTCCTT GCCTTCAGTC ATAGAAGGAC AGCCTGGTGA CCCTGGGCGA 
CCAGGTCTAG ATGGAGAACG AGGCCGCCCA GGCCCTCCTG GGCCCCCAGG CCCCCCTGGG 
CCTGCCTCAG ATCAAGGCGA CCCTGGAGAC CCTGGCTTCC CTGGAATTCC TGGCCCTCAA 
GGGCCCAAAG GAGACCAAGG AATTCCAGGT TTCTCTGGCC TCCCTGGAGA GCTAGGATTG 
AAAGGCATGA GAGGTGAGCC TGGCTTCATG GGGACTCCAG GCAAGGTTGG ACCACCTGGA 
GACCCAGGAC TTCCCGGGAT GAAGGGGAAG GCGGGGCCAA GAGGCTTATC TGGCCCTCGA 
GGTGCTCCTG GACACACACC CATTGCAGAA GCCATCCAGG CTCCCCCTGG ACCCATGGGT 
CTTCCAGGCA TTGATGGAAT CCCTGGCCTC ATGGGAGACC CTGGGGTCCA AGGACCTGTG 
GGCCTACAAG GATCTAAAGG TTTACCTGGC ATCCCTGGCA AAGATGGCGC CAGTGGACTC 
CCTGGCCCAC CTGGGGCTCT TGGTGATCCT GGTCTCCCTG GACTACAAGG CCCTCCAGGA 
TTTGAAGGAG CTCCAGGGAA GCCGGGCCCC TTCGGGAGGG CTGGAGTGCC TGGACAGAGC 
CTGAGAGTGG GCTACACCTT GGTGAAGCAC AGCCAGTCAG AACAGGTGCC CCTGTGCCCC 
AGCGGGATGA GCCAGCTCTG GGTGGGTTAC AGCCTGCTGT TCGTGGAGGG GCAAGAGAAA 
GCCCACAACC AGGACCTGGG GTTTGCTGGC TCCTGCCTGC CCCGCTTCAG CACCATGCCA 
TTCATCTACT GCAACATCAA TGAAGTGTGC CACTATGCCC GACGGAATGA TAAGTCCTAC 
TGGCTCTCCA CCACCGCCCC CATCCCTATG ATGCCGGTCG GCCAGACACA GATCCCCCAG 
TACATCAGCC GCTGCTCTGT GTGTGAGGCG CCCTCGCAAG CCATTGCTGT GCACAGCCAG 
GACATCACCA TCCCACAGTG CCCCCTGGGC TGGCGCAGTC TGTGGATTGG GTACTCCTTC 
CTCATGCACA CAGCCGCCGG TGCAGAGGGC GGGGGCCAGT CCCTGGTCTC TCCCGGATCC 
TGCCTAGAGG ATTTCCGGGC TACTCCTTTC ATCGAATGCA GTGGTGCCCG GGGCACCTGC 
CACTACTTTG CCAACAAATA TAGTTTCTGG CTGACAACAG TGGAAGAGAG GCAGCAGTTT 
GGGGAGGAGC CTGTATCTGA GACGCTGAAA GCTGGACAGC TCCACACCCG AGTCAGCCGC 
TGCCAGGTGT GTATGAAAAG CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010798761.1
CDS	270..5336
Translation	MHPGLWLLLVTLCLTEELAGAGEKKPYGKPCGGQDCSGGCKCSPEKGARGRPGPIGIQGPTGPQGLPGPSGLSGLKGERGSPGPLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYSGLPGPPGLPGQKGSKGEPVLAQGSFKGMKGESGLPGLDGITGPQGAPGSPGAVGPRGPPGLQGPPGPPGFPGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPQGFPGVSGPPGLPGFGIAGEKGEKGIPGLPGPRGPMGLEGVQGPPGSQGRKGSSGFPGLNGFPGIKGEKGGIGLPGPDIFIDTDGAVISGYPGDPGMPGLPGLKGDEGIQGLPGPPGAPGLPALPGGPGALGPQGALGLKGERGNTGRTTTGAAGLPGRDGLPGLPGLPGPPGAASETGTLQSTEPGSPGLRGERGPKGHPGLKGIKGDPGFCACDGGVPNMGPPGEPGLPGPPGLLGLPGLKGTKGDPGSGGAQGPSGDPGVFGPRGPTGPKGKKGDPTLSTRSGMPGEQGDPGPQGLPGETGAPGKDGIPGLPGLPGLLGDGAQGFPGEKGLPGLPGEKGHTGPTGPPGIGLPGAPGPRGLPGDKGVDGLPGQPGPRGVPGITLPCIIPGPYGPSGLPGAPGFPGPKGARGLPGAPGQPGLRGNKGDPGSPGLVHLPELPGFPGPHGEKGLPGFPGLPGKDGLPGKVGSPGLPGPKGAPGDILGAENGAPGEQGLQGLPGDRGFPGDSGLPGLKGLLGKSGLLGPKGERGSPGIPGRVGQPGPPGPSGLFGIKGKPGLRGPPGFPGISGYPGKKGPRGEIGLPASAGKRGLPGLKGLPGLPGPIGFLGNSGLPGATGLPGLPGLKGEKGSVGLTGFPGIPGLPGIPGASGLKGIPGSTGRVGPSGWAGSPGEKGDRGDPGPFGIPSHRPPMLNLRFKGDKGSQGSPGAIGFPGPRGDKGEAGHPGPPGLPGAPGFPSLIKGLSGRPGPPGSTGLRGLPGLKGYPGIAGFQGIPGESGSQGLSGVPGLPGASGLPGLKGDQGQTLGISGSPGPKGQPGESGFKGVKGKDGPVGDVGFPGNKGEDGKVGISGDVGLPGSLGLPGVAGMRGNPGLPGSPGHPGATGPLGPPGLIGTKGFPGLPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGSPGIGIGAPGKPGIRGPKGSQGFPGLQGAAGLPGALGLSLPSVIEGQPGDPGRPGLDGERGRPGPPGPPGPPGPASDQGDPGDPGFPGIPGPQGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGLPGMKGKAGPRGLSGPRGAPGHTPIAEAIQAPPGPMGLPGIDGIPGLMGDPGVQGPVGLQGSKGLPGIPGKDGASGLPGPPGALGDPGLPGLQGPPGFEGAPGKPGPFGRAGVPGQSLRVGYTLVKHSQSEQVPLCPSGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVGQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGEEPVSETLKAGQLHTRVSRCQVCMKSV

Target ORF information:

RefSeq Version	XM_010800459.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010800459.2

ORF Insert Sequence:

ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT 
AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA 
ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC 
TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC 
GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA 
CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT 
GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA 
CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG 
TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA 
CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA 
TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT 
CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA 
GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG 
CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC 
CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC 
CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG 
GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT 
GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA 
GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA 
CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT 
TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA 
GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA 
CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA 
AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT 
GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA 
CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA 
CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA 
CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG 
CAACCAGGCC CCCGTGGAGT TCCAGGTATC ACCTTGCCTT GTATTATTCC TGGACCGTAT 
GGTCCATCAG GACTCCCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC 
CCTGGGGCTC CAGGCCAACC TGGATTGCGT GGAAATAAAG GGGACCCCGG AAGTCCAGGA 
TTGGTTCACC TGCCTGAATT GCCAGGGTTT CCTGGACCTC ATGGAGAGAA GGGCTTACCT 
GGGTTTCCTG GGCTCCCTGG AAAAGACGGC TTGCCTGGGA AAGTTGGCAG CCCCGGCTTA 
CCAGGTCCCA AGGGAGCCCC TGGTGACATC CTTGGTGCTG AAAATGGTGC TCCGGGGGAG 
CAAGGCCTAC AGGGACTGCC TGGGGACAGA GGATTTCCTG GAGACTCTGG CCTTCCAGGA 
CTCAAGGGTT TGCTTGGGAA GTCGGGCTTG CTCGGCCCCA AAGGTGAGCG GGGCAGCCCT 
GGAATACCAG GCCGTGTGGG TCAGCCAGGA CCCCCAGGCC CTAGTGGTCT ATTTGGCATC 
AAGGGCAAAC CCGGGCTCCG CGGACCCCCA GGCTTTCCAG GCATTTCAGG ATATCCTGGA 
AAGAAAGGTC CAAGAGGCGA GATAGGTCTC CCTGCGTCAG CTGGAAAGAG AGGCCTGCCT 
GGGCTGAAAG GGCTTCCGGG ACTGCCAGGG CCAATTGGCT TCCTGGGGAA CTCAGGCTTG 
CCAGGGGCCA CTGGGTTACC AGGCCTGCCA GGTCTGAAGG GTGAGAAGGG GTCTGTTGGA 
CTGACAGGTT TTCCAGGGAT TCCAGGTCTC CCTGGTATTC CTGGAGCAAG TGGATTAAAG 
GGAATTCCTG GGTCGACAGG AAGAGTGGGG CCATCTGGAT GGGCTGGTAG CCCTGGTGAA 
AAAGGAGACA GAGGTGACCC AGGCCCCTTT GGGATACCCA GTCATCGACC TCCAATGCTG 
AACCTCCGCT TCAAAGGAGA CAAAGGTTCT CAGGGCTCAC CTGGAGCCAT TGGATTTCCT 
GGACCCAGAG GTGACAAAGG AGAGGCTGGC CACCCCGGGC CACCAGGCTT GCCTGGAGCT 
CCTGGCTTCC CCAGCCTCAT CAAGGGACTT AGTGGGAGAC CAGGCCCCCC TGGCTCCACA 
GGACTACGGG GCTTACCTGG CCTGAAGGGG TACCCTGGAA TTGCAGGTTT CCAAGGAATT 
CCAGGAGAAA GTGGTTCACA GGGTCTCAGT GGAGTTCCCG GGCTACCAGG AGCATCTGGT 
CTCCCAGGTT TAAAAGGAGA TCAAGGCCAG ACACTTGGAA TTTCTGGTAG CCCAGGACCC 
AAGGGACAAC CTGGGGAGTC TGGTTTCAAA GGTGTGAAAG GAAAAGACGG ACCAGTTGGT 
GATGTGGGTT TCCCAGGAAA CAAAGGTGAA GATGGGAAAG TTGGTATTTC TGGAGATGTT 
GGCCTTCCTG GCTCCCTAGG ACTCCCCGGA GTTGCAGGCA TGAGAGGAAA TCCAGGGCTT 
CCAGGTTCTC CAGGCCACCC AGGGGCAACT GGGCCCCTGG GTCCCCCTGG CCTAATAGGA 
ACCAAAGGTT TCCCTGGACT TCCAGGTTTA CATGGACTGA ATGGGCTTCC AGGAACCAAG 
GGGACTCATG GAACTCCAGG ACCTAGTATA ACCGGTGTGC CTGGGCCAGC TGGCTTGCCT 
GGTCCCAAAG GGGAAAAAGG TTCTCCAGGA ATTGGCATCG GAGCCCCAGG AAAGCCAGGT 
ATAAGAGGAC CCAAAGGTAG TCAAGGTTTT CCAGGTCTCC AGGGCGCCGC TGGTCTCCCC 
GGTGCACTGG GCCTCTCCTT GCCTTCAGTC ATAGAAGGAC AGCCTGGTGA CCCTGGGCGA 
CCAGGTCTAG ATGGAGAACG AGGCCGCCCA GGCCCTCCTG GGCCCCCAGG CCCCCCTGGG 
CCTGCCTCAG ATCAAGGCGA CCCTGGAGAC CCTGGCTTCC CTGGAATTCC TGGCCCTCAA 
GGGCCCAAAG GAGACCAAGG AATTCCAGGT TTCTCTGGCC TCCCTGGAGA GCTAGGATTG 
AAAGGCATGA GAGGTGAGCC TGGCTTCATG GGGACTCCAG GCAAGGTTGG ACCACCTGGA 
GACCCAGGAC TTCCCGGGAT GAAGGGGAAG GCGGGGCCAA GAGGCTTATC TGGCCCTCGA 
GGTGCTCCTG GACACACACC CATTGCAGAA GCCATCCAGG CTCCCCCTGG ACCCATGGGT 
CTTCCAGGCA TTGATGGAAT CCCTGGCCTC ATGGGAGACC CTGGGGTCCA AGGACCTGTG 
GGCCTACAAG GATCTAAAGG TTTACCTGGC ATCCCTGGCA AAGATGGCGC CAGTGGACTC 
CCTGGCCCAC CTGGGGCTCT TGGTGATCCT GGTCTCCCTG GACTACAAGG CCCTCCAGGA 
TTTGAAGGAG CTCCAGGGAA GCCGGGCCCC TTCGGGAGGG CTGGAGTGCC TGGACAGAGC 
CTGAGAGTGG GCTACACCTT GGTGAAGCAC AGCCAGTCAG AACAGGTGCC CCTGTGCCCC 
AGCGGGATGA GCCAGCTCTG GGTGGGTTAC AGCCTGCTGT TCGTGGAGGG GCAAGAGAAA 
GCCCACAACC AGGACCTGGG GTTTGCTGGC TCCTGCCTGC CCCGCTTCAG CACCATGCCA 
TTCATCTACT GCAACATCAA TGAAGTGTGC CACTATGCCC GACGGAATGA TAAGTCCTAC 
TGGCTCTCCA CCACCGCCCC CATCCCTATG ATGCCGGTCG GCCAGACACA GATCCCCCAG 
TACATCAGCC GCTGCTCTGT GTGTGAGGCG CCCTCGCAAG CCATTGCTGT GCACAGCCAG 
GACATCACCA TCCCACAGTG CCCCCTGGGC TGGCGCAGTC TGTGGATTGG GTACTCCTTC 
CTCATGCACA CAGCCGCCGG TGCAGAGGGC GGGGGCCAGT CCCTGGTCTC TCCCGGATCC 
TGCCTAGAGG ATTTCCGGGC TACTCCTTTC ATCGAATGCA GTGGTGCCCG GGGCACCTGC 
CACTACTTTG CCAACAAATA TAGTTTCTGG CTGACAACAG TGGAAGAGAG GCAGCAGTTT 
GGGGAGGAGC CTGTATCTGA GACGCTGAAA GCTGGACAGC TCCACACCCG AGTCAGCCGC 
TGCCAGGTGT GTATGAAAAG CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( XM_002699886.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002699932.2 Bos taurus collagen type IV alpha 6 chain (COL4A6), transcript variant X3, mRNA.

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CloneID	OBa35386
Clone ID Related Accession (Same CDS sequence)	XM_010800459.2 , XM_002699886.5 , XM_002699886.4
Accession Version	XM_002699886.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-6(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002699886.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT 
AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA 
ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC 
TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC 
GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA 
CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT 
GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA 
CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG 
TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA 
CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA 
TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT 
CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA 
GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG 
CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC 
CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC 
CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG 
GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT 
GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA 
GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA 
CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT 
TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA 
GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA 
CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA 
AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT 
GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA 
CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA 
CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA 
CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG 
CAACCAGGCC CCCGTGGAGT TCCAGGTATC ACCTTGCCTT GTATTATTCC TGGACCGTAT 
GGTCCATCAG GACTCCCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC 
CCTGGGGCTC CAGGCCAACC TGGATTGCGT GGAAATAAAG GGGACCCCGG AAGTCCAGGA 
TTGGTTCACC TGCCTGAATT GCCAGGGTTT CCTGGACCTC ATGGAGAGAA GGGCTTACCT 
GGGTTTCCTG GGCTCCCTGG AAAAGACGGC TTGCCTGGGA AAGTTGGCAG CCCCGGCTTA 
CCAGGTCCCA AGGGAGCCCC TGGTGACATC CTTGGTGCTG AAAATGGTGC TCCGGGGGAG 
CAAGGCCTAC AGGGACTGCC TGGGGACAGA GGATTTCCTG GAGACTCTGG CCTTCCAGGA 
CTCAAGGGTT TGCTTGGGAA GTCGGGCTTG CTCGGCCCCA AAGGTGAGCG GGGCAGCCCT 
GGAATACCAG GCCGTGTGGG TCAGCCAGGA CCCCCAGGCC CTAGTGGTCT ATTTGGCATC 
AAGGGCAAAC CCGGGCTCCG CGGACCCCCA GGCTTTCCAG GCATTTCAGG ATATCCTGGA 
AAGAAAGGTC CAAGAGGCGA GATAGGTCTC CCTGCGTCAG CTGGAAAGAG AGGCCTGCCT 
GGGCTGAAAG GGCTTCCGGG ACTGCCAGGG CCAATTGGCT TCCTGGGGAA CTCAGGCTTG 
CCAGGGGCCA CTGGGTTACC AGGCCTGCCA GGTCTGAAGG GTGAGAAGGG GTCTGTTGGA 
CTGACAGGTT TTCCAGGGAT TCCAGGTCTC CCTGGTATTC CTGGAGCAAG TGGATTAAAG 
GGAATTCCTG GGTCGACAGG AAGAGTGGGG CCATCTGGAT GGGCTGGTAG CCCTGGTGAA 
AAAGGAGACA GAGGTGACCC AGGCCCCTTT GGGATACCCA GTCATCGACC TCCAATGCTG 
AACCTCCGCT TCAAAGGAGA CAAAGGTTCT CAGGGCTCAC CTGGAGCCAT TGGATTTCCT 
GGACCCAGAG GTGACAAAGG AGAGGCTGGC CACCCCGGGC CACCAGGCTT GCCTGGAGCT 
CCTGGCTTCC CCAGCCTCAT CAAGGGACTT AGTGGGAGAC CAGGCCCCCC TGGCTCCACA 
GGACTACGGG GCTTACCTGG CCTGAAGGGG TACCCTGGAA TTGCAGGTTT CCAAGGAATT 
CCAGGAGAAA GTGGTTCACA GGGTCTCAGT GGAGTTCCCG GGCTACCAGG AGCATCTGGT 
CTCCCAGGTT TAAAAGGAGA TCAAGGCCAG ACACTTGGAA TTTCTGGTAG CCCAGGACCC 
AAGGGACAAC CTGGGGAGTC TGGTTTCAAA GGTGTGAAAG GAAAAGACGG ACCAGTTGGT 
GATGTGGGTT TCCCAGGAAA CAAAGGTGAA GATGGGAAAG TTGGTATTTC TGGAGATGTT 
GGCCTTCCTG GCTCCCTAGG ACTCCCCGGA GTTGCAGGCA TGAGAGGAAA TCCAGGGCTT 
CCAGGTTCTC CAGGCCACCC AGGGGCAACT GGGCCCCTGG GTCCCCCTGG CCTAATAGGA 
ACCAAAGGTT TCCCTGGACT TCCAGGTTTA CATGGACTGA ATGGGCTTCC AGGAACCAAG 
GGGACTCATG GAACTCCAGG ACCTAGTATA ACCGGTGTGC CTGGGCCAGC TGGCTTGCCT 
GGTCCCAAAG GGGAAAAAGG TTCTCCAGGA ATTGGCATCG GAGCCCCAGG AAAGCCAGGT 
ATAAGAGGAC CCAAAGGTAG TCAAGGTTTT CCAGGTCTCC AGGGCGCCGC TGGTCTCCCC 
GGTGCACTGG GCCTCTCCTT GCCTTCAGTC ATAGAAGGAC AGCCTGGTGA CCCTGGGCGA 
CCAGGTCTAG ATGGAGAACG AGGCCGCCCA GGCCCTCCTG GGCCCCCAGG CCCCCCTGGG 
CCTGCCTCAG ATCAAGGCGA CCCTGGAGAC CCTGGCTTCC CTGGAATTCC TGGCCCTCAA 
GGGCCCAAAG GAGACCAAGG AATTCCAGGT TTCTCTGGCC TCCCTGGAGA GCTAGGATTG 
AAAGGCATGA GAGGTGAGCC TGGCTTCATG GGGACTCCAG GCAAGGTTGG ACCACCTGGA 
GACCCAGGAC TTCCCGGGAT GAAGGGGAAG GCGGGGCCAA GAGGCTTATC TGGCCCTCGA 
GGTGCTCCTG GACACACACC CATTGCAGAA GCCATCCAGG CTCCCCCTGG ACCCATGGGT 
CTTCCAGGCA TTGATGGAAT CCCTGGCCTC ATGGGAGACC CTGGGGTCCA AGGACCTGTG 
GGCCTACAAG GATCTAAAGG TTTACCTGGC ATCCCTGGCA AAGATGGCGC CAGTGGACTC 
CCTGGCCCAC CTGGGGCTCT TGGTGATCCT GGTCTCCCTG GACTACAAGG CCCTCCAGGA 
TTTGAAGGAG CTCCAGGGAA GCCGGGCCCC TTCGGGAGGG CTGGAGTGCC TGGACAGAGC 
CTGAGAGTGG GCTACACCTT GGTGAAGCAC AGCCAGTCAG AACAGGTGCC CCTGTGCCCC 
AGCGGGATGA GCCAGCTCTG GGTGGGTTAC AGCCTGCTGT TCGTGGAGGG GCAAGAGAAA 
GCCCACAACC AGGACCTGGG GTTTGCTGGC TCCTGCCTGC CCCGCTTCAG CACCATGCCA 
TTCATCTACT GCAACATCAA TGAAGTGTGC CACTATGCCC GACGGAATGA TAAGTCCTAC 
TGGCTCTCCA CCACCGCCCC CATCCCTATG ATGCCGGTCG GCCAGACACA GATCCCCCAG 
TACATCAGCC GCTGCTCTGT GTGTGAGGCG CCCTCGCAAG CCATTGCTGT GCACAGCCAG 
GACATCACCA TCCCACAGTG CCCCCTGGGC TGGCGCAGTC TGTGGATTGG GTACTCCTTC 
CTCATGCACA CAGCCGCCGG TGCAGAGGGC GGGGGCCAGT CCCTGGTCTC TCCCGGATCC 
TGCCTAGAGG ATTTCCGGGC TACTCCTTTC ATCGAATGCA GTGGTGCCCG GGGCACCTGC 
CACTACTTTG CCAACAAATA TAGTTTCTGG CTGACAACAG TGGAAGAGAG GCAGCAGTTT 
GGGGAGGAGC CTGTATCTGA GACGCTGAAA GCTGGACAGC TCCACACCCG AGTCAGCCGC 
TGCCAGGTGT GTATGAAAAG CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002699932.2
CDS	256..5322
Translation	MHPGLWLLLVTLCLTEELAGAGEKKPYGKPCGGQDCSGGCKCSPEKGARGRPGPIGIQGPTGPQGLPGPSGLSGLKGERGSPGPLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYSGLPGPPGLPGQKGSKGEPVLAQGSFKGMKGESGLPGLDGITGPQGAPGSPGAVGPRGPPGLQGPPGPPGFPGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPQGFPGVSGPPGLPGFGIAGEKGEKGIPGLPGPRGPMGLEGVQGPPGSQGRKGSSGFPGLNGFPGIKGEKGGIGLPGPDIFIDTDGAVISGYPGDPGMPGLPGLKGDEGIQGLPGPPGAPGLPALPGGPGALGPQGALGLKGERGNTGRTTTGAAGLPGRDGLPGLPGLPGPPGAASETGTLQSTEPGSPGLRGERGPKGHPGLKGIKGDPGFCACDGGVPNMGPPGEPGLPGPPGLLGLPGLKGTKGDPGSGGAQGPSGDPGVFGPRGPTGPKGKKGDPTLSTRSGMPGEQGDPGPQGLPGETGAPGKDGIPGLPGLPGLLGDGAQGFPGEKGLPGLPGEKGHTGPTGPPGIGLPGAPGPRGLPGDKGVDGLPGQPGPRGVPGITLPCIIPGPYGPSGLPGAPGFPGPKGARGLPGAPGQPGLRGNKGDPGSPGLVHLPELPGFPGPHGEKGLPGFPGLPGKDGLPGKVGSPGLPGPKGAPGDILGAENGAPGEQGLQGLPGDRGFPGDSGLPGLKGLLGKSGLLGPKGERGSPGIPGRVGQPGPPGPSGLFGIKGKPGLRGPPGFPGISGYPGKKGPRGEIGLPASAGKRGLPGLKGLPGLPGPIGFLGNSGLPGATGLPGLPGLKGEKGSVGLTGFPGIPGLPGIPGASGLKGIPGSTGRVGPSGWAGSPGEKGDRGDPGPFGIPSHRPPMLNLRFKGDKGSQGSPGAIGFPGPRGDKGEAGHPGPPGLPGAPGFPSLIKGLSGRPGPPGSTGLRGLPGLKGYPGIAGFQGIPGESGSQGLSGVPGLPGASGLPGLKGDQGQTLGISGSPGPKGQPGESGFKGVKGKDGPVGDVGFPGNKGEDGKVGISGDVGLPGSLGLPGVAGMRGNPGLPGSPGHPGATGPLGPPGLIGTKGFPGLPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGSPGIGIGAPGKPGIRGPKGSQGFPGLQGAAGLPGALGLSLPSVIEGQPGDPGRPGLDGERGRPGPPGPPGPPGPASDQGDPGDPGFPGIPGPQGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGLPGMKGKAGPRGLSGPRGAPGHTPIAEAIQAPPGPMGLPGIDGIPGLMGDPGVQGPVGLQGSKGLPGIPGKDGASGLPGPPGALGDPGLPGLQGPPGFEGAPGKPGPFGRAGVPGQSLRVGYTLVKHSQSEQVPLCPSGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVGQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGEEPVSETLKAGQLHTRVSRCQVCMKSV

Target ORF information:

RefSeq Version	XM_002699886.5
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type IV alpha 6 chain (COL4A6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002699886.5

ORF Insert Sequence:

ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT 
AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA 
ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC 
TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC 
GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA 
CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT 
GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA 
CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG 
TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA 
CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA 
TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT 
CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA 
GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG 
CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC 
CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC 
CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG 
GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT 
GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA 
GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA 
CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT 
TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA 
GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA 
CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA 
AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT 
GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA 
CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA 
CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA 
CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG 
CAACCAGGCC CCCGTGGAGT TCCAGGTATC ACCTTGCCTT GTATTATTCC TGGACCGTAT 
GGTCCATCAG GACTCCCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC 
CCTGGGGCTC CAGGCCAACC TGGATTGCGT GGAAATAAAG GGGACCCCGG AAGTCCAGGA 
TTGGTTCACC TGCCTGAATT GCCAGGGTTT CCTGGACCTC ATGGAGAGAA GGGCTTACCT 
GGGTTTCCTG GGCTCCCTGG AAAAGACGGC TTGCCTGGGA AAGTTGGCAG CCCCGGCTTA 
CCAGGTCCCA AGGGAGCCCC TGGTGACATC CTTGGTGCTG AAAATGGTGC TCCGGGGGAG 
CAAGGCCTAC AGGGACTGCC TGGGGACAGA GGATTTCCTG GAGACTCTGG CCTTCCAGGA 
CTCAAGGGTT TGCTTGGGAA GTCGGGCTTG CTCGGCCCCA AAGGTGAGCG GGGCAGCCCT 
GGAATACCAG GCCGTGTGGG TCAGCCAGGA CCCCCAGGCC CTAGTGGTCT ATTTGGCATC 
AAGGGCAAAC CCGGGCTCCG CGGACCCCCA GGCTTTCCAG GCATTTCAGG ATATCCTGGA 
AAGAAAGGTC CAAGAGGCGA GATAGGTCTC CCTGCGTCAG CTGGAAAGAG AGGCCTGCCT 
GGGCTGAAAG GGCTTCCGGG ACTGCCAGGG CCAATTGGCT TCCTGGGGAA CTCAGGCTTG 
CCAGGGGCCA CTGGGTTACC AGGCCTGCCA GGTCTGAAGG GTGAGAAGGG GTCTGTTGGA 
CTGACAGGTT TTCCAGGGAT TCCAGGTCTC CCTGGTATTC CTGGAGCAAG TGGATTAAAG 
GGAATTCCTG GGTCGACAGG AAGAGTGGGG CCATCTGGAT GGGCTGGTAG CCCTGGTGAA 
AAAGGAGACA GAGGTGACCC AGGCCCCTTT GGGATACCCA GTCATCGACC TCCAATGCTG 
AACCTCCGCT TCAAAGGAGA CAAAGGTTCT CAGGGCTCAC CTGGAGCCAT TGGATTTCCT 
GGACCCAGAG GTGACAAAGG AGAGGCTGGC CACCCCGGGC CACCAGGCTT GCCTGGAGCT 
CCTGGCTTCC CCAGCCTCAT CAAGGGACTT AGTGGGAGAC CAGGCCCCCC TGGCTCCACA 
GGACTACGGG GCTTACCTGG CCTGAAGGGG TACCCTGGAA TTGCAGGTTT CCAAGGAATT 
CCAGGAGAAA GTGGTTCACA GGGTCTCAGT GGAGTTCCCG GGCTACCAGG AGCATCTGGT 
CTCCCAGGTT TAAAAGGAGA TCAAGGCCAG ACACTTGGAA TTTCTGGTAG CCCAGGACCC 
AAGGGACAAC CTGGGGAGTC TGGTTTCAAA GGTGTGAAAG GAAAAGACGG ACCAGTTGGT 
GATGTGGGTT TCCCAGGAAA CAAAGGTGAA GATGGGAAAG TTGGTATTTC TGGAGATGTT 
GGCCTTCCTG GCTCCCTAGG ACTCCCCGGA GTTGCAGGCA TGAGAGGAAA TCCAGGGCTT 
CCAGGTTCTC CAGGCCACCC AGGGGCAACT GGGCCCCTGG GTCCCCCTGG CCTAATAGGA 
ACCAAAGGTT TCCCTGGACT TCCAGGTTTA CATGGACTGA ATGGGCTTCC AGGAACCAAG 
GGGACTCATG GAACTCCAGG ACCTAGTATA ACCGGTGTGC CTGGGCCAGC TGGCTTGCCT 
GGTCCCAAAG GGGAAAAAGG TTCTCCAGGA ATTGGCATCG GAGCCCCAGG AAAGCCAGGT 
ATAAGAGGAC CCAAAGGTAG TCAAGGTTTT CCAGGTCTCC AGGGCGCCGC TGGTCTCCCC 
GGTGCACTGG GCCTCTCCTT GCCTTCAGTC ATAGAAGGAC AGCCTGGTGA CCCTGGGCGA 
CCAGGTCTAG ATGGAGAACG AGGCCGCCCA GGCCCTCCTG GGCCCCCAGG CCCCCCTGGG 
CCTGCCTCAG ATCAAGGCGA CCCTGGAGAC CCTGGCTTCC CTGGAATTCC TGGCCCTCAA 
GGGCCCAAAG GAGACCAAGG AATTCCAGGT TTCTCTGGCC TCCCTGGAGA GCTAGGATTG 
AAAGGCATGA GAGGTGAGCC TGGCTTCATG GGGACTCCAG GCAAGGTTGG ACCACCTGGA 
GACCCAGGAC TTCCCGGGAT GAAGGGGAAG GCGGGGCCAA GAGGCTTATC TGGCCCTCGA 
GGTGCTCCTG GACACACACC CATTGCAGAA GCCATCCAGG CTCCCCCTGG ACCCATGGGT 
CTTCCAGGCA TTGATGGAAT CCCTGGCCTC ATGGGAGACC CTGGGGTCCA AGGACCTGTG 
GGCCTACAAG GATCTAAAGG TTTACCTGGC ATCCCTGGCA AAGATGGCGC CAGTGGACTC 
CCTGGCCCAC CTGGGGCTCT TGGTGATCCT GGTCTCCCTG GACTACAAGG CCCTCCAGGA 
TTTGAAGGAG CTCCAGGGAA GCCGGGCCCC TTCGGGAGGG CTGGAGTGCC TGGACAGAGC 
CTGAGAGTGG GCTACACCTT GGTGAAGCAC AGCCAGTCAG AACAGGTGCC CCTGTGCCCC 
AGCGGGATGA GCCAGCTCTG GGTGGGTTAC AGCCTGCTGT TCGTGGAGGG GCAAGAGAAA 
GCCCACAACC AGGACCTGGG GTTTGCTGGC TCCTGCCTGC CCCGCTTCAG CACCATGCCA 
TTCATCTACT GCAACATCAA TGAAGTGTGC CACTATGCCC GACGGAATGA TAAGTCCTAC 
TGGCTCTCCA CCACCGCCCC CATCCCTATG ATGCCGGTCG GCCAGACACA GATCCCCCAG 
TACATCAGCC GCTGCTCTGT GTGTGAGGCG CCCTCGCAAG CCATTGCTGT GCACAGCCAG 
GACATCACCA TCCCACAGTG CCCCCTGGGC TGGCGCAGTC TGTGGATTGG GTACTCCTTC 
CTCATGCACA CAGCCGCCGG TGCAGAGGGC GGGGGCCAGT CCCTGGTCTC TCCCGGATCC 
TGCCTAGAGG ATTTCCGGGC TACTCCTTTC ATCGAATGCA GTGGTGCCCG GGGCACCTGC 
CACTACTTTG CCAACAAATA TAGTTTCTGG CTGACAACAG TGGAAGAGAG GCAGCAGTTT 
GGGGAGGAGC CTGTATCTGA GACGCTGAAA GCTGGACAGC TCCACACCCG AGTCAGCCGC 
TGCCAGGTGT GTATGAAAAG CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A6 ( XM_002699886.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002699932.2 Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X2, mRNA.

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CloneID	OBa35386
Clone ID Related Accession (Same CDS sequence)	XM_010800459.2 , XM_002699886.5 , XM_002699886.4
Accession Version	XM_002699886.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-6(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104715.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002699886.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT 
AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA 
ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC 
TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC 
GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA 
CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT 
GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA 
CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG 
TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA 
CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA 
TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT 
CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA 
GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG 
CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC 
CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC 
CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG 
GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT 
GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA 
GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA 
CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT 
TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA 
GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA 
CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA 
AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT 
GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA 
CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA 
CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA 
CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG 
CAACCAGGCC CCCGTGGAGT TCCAGGTATC ACCTTGCCTT GTATTATTCC TGGACCGTAT 
GGTCCATCAG GACTCCCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC 
CCTGGGGCTC CAGGCCAACC TGGATTGCGT GGAAATAAAG GGGACCCCGG AAGTCCAGGA 
TTGGTTCACC TGCCTGAATT GCCAGGGTTT CCTGGACCTC ATGGAGAGAA GGGCTTACCT 
GGGTTTCCTG GGCTCCCTGG AAAAGACGGC TTGCCTGGGA AAGTTGGCAG CCCCGGCTTA 
CCAGGTCCCA AGGGAGCCCC TGGTGACATC CTTGGTGCTG AAAATGGTGC TCCGGGGGAG 
CAAGGCCTAC AGGGACTGCC TGGGGACAGA GGATTTCCTG GAGACTCTGG CCTTCCAGGA 
CTCAAGGGTT TGCTTGGGAA GTCGGGCTTG CTCGGCCCCA AAGGTGAGCG GGGCAGCCCT 
GGAATACCAG GCCGTGTGGG TCAGCCAGGA CCCCCAGGCC CTAGTGGTCT ATTTGGCATC 
AAGGGCAAAC CCGGGCTCCG CGGACCCCCA GGCTTTCCAG GCATTTCAGG ATATCCTGGA 
AAGAAAGGTC CAAGAGGCGA GATAGGTCTC CCTGCGTCAG CTGGAAAGAG AGGCCTGCCT 
GGGCTGAAAG GGCTTCCGGG ACTGCCAGGG CCAATTGGCT TCCTGGGGAA CTCAGGCTTG 
CCAGGGGCCA CTGGGTTACC AGGCCTGCCA GGTCTGAAGG GTGAGAAGGG GTCTGTTGGA 
CTGACAGGTT TTCCAGGGAT TCCAGGTCTC CCTGGTATTC CTGGAGCAAG TGGATTAAAG 
GGAATTCCTG GGTCGACAGG AAGAGTGGGG CCATCTGGAT GGGCTGGTAG CCCTGGTGAA 
AAAGGAGACA GAGGTGACCC AGGCCCCTTT GGGATACCCA GTCATCGACC TCCAATGCTG 
AACCTCCGCT TCAAAGGAGA CAAAGGTTCT CAGGGCTCAC CTGGAGCCAT TGGATTTCCT 
GGACCCAGAG GTGACAAAGG AGAGGCTGGC CACCCCGGGC CACCAGGCTT GCCTGGAGCT 
CCTGGCTTCC CCAGCCTCAT CAAGGGACTT AGTGGGAGAC CAGGCCCCCC TGGCTCCACA 
GGACTACGGG GCTTACCTGG CCTGAAGGGG TACCCTGGAA TTGCAGGTTT CCAAGGAATT 
CCAGGAGAAA GTGGTTCACA GGGTCTCAGT GGAGTTCCCG GGCTACCAGG AGCATCTGGT 
CTCCCAGGTT TAAAAGGAGA TCAAGGCCAG ACACTTGGAA TTTCTGGTAG CCCAGGACCC 
AAGGGACAAC CTGGGGAGTC TGGTTTCAAA GGTGTGAAAG GAAAAGACGG ACCAGTTGGT 
GATGTGGGTT TCCCAGGAAA CAAAGGTGAA GATGGGAAAG TTGGTATTTC TGGAGATGTT 
GGCCTTCCTG GCTCCCTAGG ACTCCCCGGA GTTGCAGGCA TGAGAGGAAA TCCAGGGCTT 
CCAGGTTCTC CAGGCCACCC AGGGGCAACT GGGCCCCTGG GTCCCCCTGG CCTAATAGGA 
ACCAAAGGTT TCCCTGGACT TCCAGGTTTA CATGGACTGA ATGGGCTTCC AGGAACCAAG 
GGGACTCATG GAACTCCAGG ACCTAGTATA ACCGGTGTGC CTGGGCCAGC TGGCTTGCCT 
GGTCCCAAAG GGGAAAAAGG TTCTCCAGGA ATTGGCATCG GAGCCCCAGG AAAGCCAGGT 
ATAAGAGGAC CCAAAGGTAG TCAAGGTTTT CCAGGTCTCC AGGGCGCCGC TGGTCTCCCC 
GGTGCACTGG GCCTCTCCTT GCCTTCAGTC ATAGAAGGAC AGCCTGGTGA CCCTGGGCGA 
CCAGGTCTAG ATGGAGAACG AGGCCGCCCA GGCCCTCCTG GGCCCCCAGG CCCCCCTGGG 
CCTGCCTCAG ATCAAGGCGA CCCTGGAGAC CCTGGCTTCC CTGGAATTCC TGGCCCTCAA 
GGGCCCAAAG GAGACCAAGG AATTCCAGGT TTCTCTGGCC TCCCTGGAGA GCTAGGATTG 
AAAGGCATGA GAGGTGAGCC TGGCTTCATG GGGACTCCAG GCAAGGTTGG ACCACCTGGA 
GACCCAGGAC TTCCCGGGAT GAAGGGGAAG GCGGGGCCAA GAGGCTTATC TGGCCCTCGA 
GGTGCTCCTG GACACACACC CATTGCAGAA GCCATCCAGG CTCCCCCTGG ACCCATGGGT 
CTTCCAGGCA TTGATGGAAT CCCTGGCCTC ATGGGAGACC CTGGGGTCCA AGGACCTGTG 
GGCCTACAAG GATCTAAAGG TTTACCTGGC ATCCCTGGCA AAGATGGCGC CAGTGGACTC 
CCTGGCCCAC CTGGGGCTCT TGGTGATCCT GGTCTCCCTG GACTACAAGG CCCTCCAGGA 
TTTGAAGGAG CTCCAGGGAA GCCGGGCCCC TTCGGGAGGG CTGGAGTGCC TGGACAGAGC 
CTGAGAGTGG GCTACACCTT GGTGAAGCAC AGCCAGTCAG AACAGGTGCC CCTGTGCCCC 
AGCGGGATGA GCCAGCTCTG GGTGGGTTAC AGCCTGCTGT TCGTGGAGGG GCAAGAGAAA 
GCCCACAACC AGGACCTGGG GTTTGCTGGC TCCTGCCTGC CCCGCTTCAG CACCATGCCA 
TTCATCTACT GCAACATCAA TGAAGTGTGC CACTATGCCC GACGGAATGA TAAGTCCTAC 
TGGCTCTCCA CCACCGCCCC CATCCCTATG ATGCCGGTCG GCCAGACACA GATCCCCCAG 
TACATCAGCC GCTGCTCTGT GTGTGAGGCG CCCTCGCAAG CCATTGCTGT GCACAGCCAG 
GACATCACCA TCCCACAGTG CCCCCTGGGC TGGCGCAGTC TGTGGATTGG GTACTCCTTC 
CTCATGCACA CAGCCGCCGG TGCAGAGGGC GGGGGCCAGT CCCTGGTCTC TCCCGGATCC 
TGCCTAGAGG ATTTCCGGGC TACTCCTTTC ATCGAATGCA GTGGTGCCCG GGGCACCTGC 
CACTACTTTG CCAACAAATA TAGTTTCTGG CTGACAACAG TGGAAGAGAG GCAGCAGTTT 
GGGGAGGAGC CTGTATCTGA GACGCTGAAA GCTGGACAGC TCCACACCCG AGTCAGCCGC 
TGCCAGGTGT GTATGAAAAG CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002699932.2
CDS	270..5336
Translation	MHPGLWLLLVTLCLTEELAGAGEKKPYGKPCGGQDCSGGCKCSPEKGARGRPGPIGIQGPTGPQGLPGPSGLSGLKGERGSPGPLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYSGLPGPPGLPGQKGSKGEPVLAQGSFKGMKGESGLPGLDGITGPQGAPGSPGAVGPRGPPGLQGPPGPPGFPGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPQGFPGVSGPPGLPGFGIAGEKGEKGIPGLPGPRGPMGLEGVQGPPGSQGRKGSSGFPGLNGFPGIKGEKGGIGLPGPDIFIDTDGAVISGYPGDPGMPGLPGLKGDEGIQGLPGPPGAPGLPALPGGPGALGPQGALGLKGERGNTGRTTTGAAGLPGRDGLPGLPGLPGPPGAASETGTLQSTEPGSPGLRGERGPKGHPGLKGIKGDPGFCACDGGVPNMGPPGEPGLPGPPGLLGLPGLKGTKGDPGSGGAQGPSGDPGVFGPRGPTGPKGKKGDPTLSTRSGMPGEQGDPGPQGLPGETGAPGKDGIPGLPGLPGLLGDGAQGFPGEKGLPGLPGEKGHTGPTGPPGIGLPGAPGPRGLPGDKGVDGLPGQPGPRGVPGITLPCIIPGPYGPSGLPGAPGFPGPKGARGLPGAPGQPGLRGNKGDPGSPGLVHLPELPGFPGPHGEKGLPGFPGLPGKDGLPGKVGSPGLPGPKGAPGDILGAENGAPGEQGLQGLPGDRGFPGDSGLPGLKGLLGKSGLLGPKGERGSPGIPGRVGQPGPPGPSGLFGIKGKPGLRGPPGFPGISGYPGKKGPRGEIGLPASAGKRGLPGLKGLPGLPGPIGFLGNSGLPGATGLPGLPGLKGEKGSVGLTGFPGIPGLPGIPGASGLKGIPGSTGRVGPSGWAGSPGEKGDRGDPGPFGIPSHRPPMLNLRFKGDKGSQGSPGAIGFPGPRGDKGEAGHPGPPGLPGAPGFPSLIKGLSGRPGPPGSTGLRGLPGLKGYPGIAGFQGIPGESGSQGLSGVPGLPGASGLPGLKGDQGQTLGISGSPGPKGQPGESGFKGVKGKDGPVGDVGFPGNKGEDGKVGISGDVGLPGSLGLPGVAGMRGNPGLPGSPGHPGATGPLGPPGLIGTKGFPGLPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGSPGIGIGAPGKPGIRGPKGSQGFPGLQGAAGLPGALGLSLPSVIEGQPGDPGRPGLDGERGRPGPPGPPGPPGPASDQGDPGDPGFPGIPGPQGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGLPGMKGKAGPRGLSGPRGAPGHTPIAEAIQAPPGPMGLPGIDGIPGLMGDPGVQGPVGLQGSKGLPGIPGKDGASGLPGPPGALGDPGLPGLQGPPGFEGAPGKPGPFGRAGVPGQSLRVGYTLVKHSQSEQVPLCPSGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVGQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGEEPVSETLKAGQLHTRVSRCQVCMKSV

Target ORF information:

RefSeq Version	XM_002699886.4
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002699886.4

ORF Insert Sequence:

ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT 
AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA 
ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC 
TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC 
GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA 
CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC 
TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT 
GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT 
GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA 
CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG 
TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA 
CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC 
AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA 
TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT 
CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA 
GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG 
CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC 
CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC 
CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG 
GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT 
GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA 
GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA 
CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT 
TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA 
GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA 
CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA 
AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT 
GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA 
CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA 
CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA 
CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG 
CAACCAGGCC CCCGTGGAGT TCCAGGTATC ACCTTGCCTT GTATTATTCC TGGACCGTAT 
GGTCCATCAG GACTCCCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC 
CCTGGGGCTC CAGGCCAACC TGGATTGCGT GGAAATAAAG GGGACCCCGG AAGTCCAGGA 
TTGGTTCACC TGCCTGAATT GCCAGGGTTT CCTGGACCTC ATGGAGAGAA GGGCTTACCT 
GGGTTTCCTG GGCTCCCTGG AAAAGACGGC TTGCCTGGGA AAGTTGGCAG CCCCGGCTTA 
CCAGGTCCCA AGGGAGCCCC TGGTGACATC CTTGGTGCTG AAAATGGTGC TCCGGGGGAG 
CAAGGCCTAC AGGGACTGCC TGGGGACAGA GGATTTCCTG GAGACTCTGG CCTTCCAGGA 
CTCAAGGGTT TGCTTGGGAA GTCGGGCTTG CTCGGCCCCA AAGGTGAGCG GGGCAGCCCT 
GGAATACCAG GCCGTGTGGG TCAGCCAGGA CCCCCAGGCC CTAGTGGTCT ATTTGGCATC 
AAGGGCAAAC CCGGGCTCCG CGGACCCCCA GGCTTTCCAG GCATTTCAGG ATATCCTGGA 
AAGAAAGGTC CAAGAGGCGA GATAGGTCTC CCTGCGTCAG CTGGAAAGAG AGGCCTGCCT 
GGGCTGAAAG GGCTTCCGGG ACTGCCAGGG CCAATTGGCT TCCTGGGGAA CTCAGGCTTG 
CCAGGGGCCA CTGGGTTACC AGGCCTGCCA GGTCTGAAGG GTGAGAAGGG GTCTGTTGGA 
CTGACAGGTT TTCCAGGGAT TCCAGGTCTC CCTGGTATTC CTGGAGCAAG TGGATTAAAG 
GGAATTCCTG GGTCGACAGG AAGAGTGGGG CCATCTGGAT GGGCTGGTAG CCCTGGTGAA 
AAAGGAGACA GAGGTGACCC AGGCCCCTTT GGGATACCCA GTCATCGACC TCCAATGCTG 
AACCTCCGCT TCAAAGGAGA CAAAGGTTCT CAGGGCTCAC CTGGAGCCAT TGGATTTCCT 
GGACCCAGAG GTGACAAAGG AGAGGCTGGC CACCCCGGGC CACCAGGCTT GCCTGGAGCT 
CCTGGCTTCC CCAGCCTCAT CAAGGGACTT AGTGGGAGAC CAGGCCCCCC TGGCTCCACA 
GGACTACGGG GCTTACCTGG CCTGAAGGGG TACCCTGGAA TTGCAGGTTT CCAAGGAATT 
CCAGGAGAAA GTGGTTCACA GGGTCTCAGT GGAGTTCCCG GGCTACCAGG AGCATCTGGT 
CTCCCAGGTT TAAAAGGAGA TCAAGGCCAG ACACTTGGAA TTTCTGGTAG CCCAGGACCC 
AAGGGACAAC CTGGGGAGTC TGGTTTCAAA GGTGTGAAAG GAAAAGACGG ACCAGTTGGT 
GATGTGGGTT TCCCAGGAAA CAAAGGTGAA GATGGGAAAG TTGGTATTTC TGGAGATGTT 
GGCCTTCCTG GCTCCCTAGG ACTCCCCGGA GTTGCAGGCA TGAGAGGAAA TCCAGGGCTT 
CCAGGTTCTC CAGGCCACCC AGGGGCAACT GGGCCCCTGG GTCCCCCTGG CCTAATAGGA 
ACCAAAGGTT TCCCTGGACT TCCAGGTTTA CATGGACTGA ATGGGCTTCC AGGAACCAAG 
GGGACTCATG GAACTCCAGG ACCTAGTATA ACCGGTGTGC CTGGGCCAGC TGGCTTGCCT 
GGTCCCAAAG GGGAAAAAGG TTCTCCAGGA ATTGGCATCG GAGCCCCAGG AAAGCCAGGT 
ATAAGAGGAC CCAAAGGTAG TCAAGGTTTT CCAGGTCTCC AGGGCGCCGC TGGTCTCCCC 
GGTGCACTGG GCCTCTCCTT GCCTTCAGTC ATAGAAGGAC AGCCTGGTGA CCCTGGGCGA 
CCAGGTCTAG ATGGAGAACG AGGCCGCCCA GGCCCTCCTG GGCCCCCAGG CCCCCCTGGG 
CCTGCCTCAG ATCAAGGCGA CCCTGGAGAC CCTGGCTTCC CTGGAATTCC TGGCCCTCAA 
GGGCCCAAAG GAGACCAAGG AATTCCAGGT TTCTCTGGCC TCCCTGGAGA GCTAGGATTG 
AAAGGCATGA GAGGTGAGCC TGGCTTCATG GGGACTCCAG GCAAGGTTGG ACCACCTGGA 
GACCCAGGAC TTCCCGGGAT GAAGGGGAAG GCGGGGCCAA GAGGCTTATC TGGCCCTCGA 
GGTGCTCCTG GACACACACC CATTGCAGAA GCCATCCAGG CTCCCCCTGG ACCCATGGGT 
CTTCCAGGCA TTGATGGAAT CCCTGGCCTC ATGGGAGACC CTGGGGTCCA AGGACCTGTG 
GGCCTACAAG GATCTAAAGG TTTACCTGGC ATCCCTGGCA AAGATGGCGC CAGTGGACTC 
CCTGGCCCAC CTGGGGCTCT TGGTGATCCT GGTCTCCCTG GACTACAAGG CCCTCCAGGA 
TTTGAAGGAG CTCCAGGGAA GCCGGGCCCC TTCGGGAGGG CTGGAGTGCC TGGACAGAGC 
CTGAGAGTGG GCTACACCTT GGTGAAGCAC AGCCAGTCAG AACAGGTGCC CCTGTGCCCC 
AGCGGGATGA GCCAGCTCTG GGTGGGTTAC AGCCTGCTGT TCGTGGAGGG GCAAGAGAAA 
GCCCACAACC AGGACCTGGG GTTTGCTGGC TCCTGCCTGC CCCGCTTCAG CACCATGCCA 
TTCATCTACT GCAACATCAA TGAAGTGTGC CACTATGCCC GACGGAATGA TAAGTCCTAC 
TGGCTCTCCA CCACCGCCCC CATCCCTATG ATGCCGGTCG GCCAGACACA GATCCCCCAG 
TACATCAGCC GCTGCTCTGT GTGTGAGGCG CCCTCGCAAG CCATTGCTGT GCACAGCCAG 
GACATCACCA TCCCACAGTG CCCCCTGGGC TGGCGCAGTC TGTGGATTGG GTACTCCTTC 
CTCATGCACA CAGCCGCCGG TGCAGAGGGC GGGGGCCAGT CCCTGGTCTC TCCCGGATCC 
TGCCTAGAGG ATTTCCGGGC TACTCCTTTC ATCGAATGCA GTGGTGCCCG GGGCACCTGC 
CACTACTTTG CCAACAAATA TAGTTTCTGG CTGACAACAG TGGAAGAGAG GCAGCAGTTT 
GGGGAGGAGC CTGTATCTGA GACGCTGAAA GCTGGACAGC TCCACACCCG AGTCAGCCGC 
TGCCAGGTGT GTATGAAAAG CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

COL4A6 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報