COL4A6 cDNA ORF clone, Bos taurus(cattle)

The following COL4A6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa238803 XM_024988805.1
Latest version!
Bos taurus collagen type IV alpha 6 chain (COL4A6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa12979 XM_005227897.4
Latest version!
Bos taurus collagen type IV alpha 6 chain (COL4A6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa12979 XM_601826.8
Latest version!
Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa35386 XM_002699886.5
Latest version!
Bos taurus collagen type IV alpha 6 chain (COL4A6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa35386 XM_010800459.2
Latest version!
Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
View Old Accession Versions >>
OBa35386 XM_002699886.4 Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa12979 XM_005227897.3 Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa238803
    Clone ID Related Accession (Same CDS sequence) XM_024988805.1
    Accession Version XM_024988805.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-6(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
    GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT
    AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA
    ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC
    TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGCCACCC TGGACAGCCA
    GGACCCAGAG GCCCACCTGG CCTGGATGGC TGTAATGGAA CTCAAGGAGC TGTTGGATTT
    CCAGGCCCTG ATGGCTATTC TGGGCTTCCT GGACCACCTG GGCTTCCTGG TCAGAAAGGC
    TCTAAAGGTG AACCTGTCCT TGCCCAAGGT AGTTTCAAAG GAATGAAGGG GGAGTCTGGG
    CTGCCCGGAC TGGATGGAAT CACTGGCCCA CAAGGAGCAC CCGGATCTCC AGGAGCTGTA
    GGACCCAGAG GACCACCAGG ATTGCAAGGT CCTCCTGGCC CCCCTGGTTT CCCTGGTCCT
    GATGGGAATA TGGGGTTAGG TTTCCAAGGA GAGAAAGGAG TCAAGGGAGA TGTTGGCCTG
    CCTGGTCCTG CAGGACCCCC ACCATCTACT GGAGAACTGG AATTCATGGG ATTTCCCAAA
    GGGAAGAAAG GATCCAAGGG TGAACCAGGG CCTCAGGGCT TCCCAGGAGT AAGTGGTCCA
    CCTGGTCTGC CGGGATTTGG AATTGCTGGA GAAAAAGGAG AAAAGGGAAT TCCTGGTTTG
    CCAGGACCTA GGGGTCCCAT GGGTTTAGAA GGAGTCCAAG GCCCTCCGGG GAGTCAGGGC
    AGGAAGGGGT CTTCAGGTTT CCCCGGGCTT AATGGATTCC CAGGAATTAA GGGTGAAAAA
    GGTGGCATTG GCCTGCCAGG CCCAGATATT TTCATCGATA CAGATGGTGC TGTGATCTCA
    GGTTATCCTG GAGACCCTGG TATGCCAGGC CTCCCAGGCC TTAAAGGAGA TGAAGGCATA
    CAGGGCCTAC CTGGCCCTCC TGGCGCCCCT GGATTACCAG CTTTACCAGG TGGCCCCGGT
    GCTCTGGGGC CCCAGGGAGC TCTAGGCCTG AAGGGGGAAC GAGGAAACAC TGGCCGGACC
    ACAACGGGGG CAGCTGGTCT CCCTGGCAGA GATGGTTTGC CAGGCCTGCC AGGCCTACCA
    GGCCCACCTG GTGCAGCATC TGAGACGGGA ACCCTACAGA GCACAGAACC GGGATCGCCG
    GGACTCCGAG GGGAACGAGG TCCAAAAGGA CACCCAGGCC TCAAAGGAAT TAAAGGGGAC
    CCAGGTTTTT GTGCTTGTGA TGGTGGTGTC CCCAACATGG GGCCACCTGG GGAGCCAGGC
    CTGCCTGGGC CACCAGGACT TCTGGGCCTT CCAGGTCTTA AAGGAACCAA AGGTGATCCA
    GGCTCTGGGG GTGCACAGGG CCCATCAGGG GATCCAGGTG TATTTGGGCC TCGAGGTCCA
    ACAGGCCCTA AAGGAAAGAA AGGGGACCCA ACTCTCAGCA CAAGATCAGG GATGCCAGGA
    GAGCAAGGTG ATCCTGGCCC CCAGGGGCTC CCTGGTGAGA CAGGAGCCCC AGGCAAGGAT
    GGAATACCAG GTTTACCAGG GCTGCCAGGC CTTCTGGGTG ATGGTGCACA GGGCTTCCCC
    GGTGAAAAGG GGTTACCAGG ACTTCCTGGT GAAAAAGGCC ACACTGGTCC AACTGGCCCC
    CCAGGAATTG GGCTACCAGG GGCTCCTGGA CCTCGTGGAC TTCCTGGAGA TAAAGGAGTA
    GATGGATTAC CAGGGCAACC AGGCCCCCGT GGAGTTCCAG GTGACTGCTG CTGCAAGGAG
    GAGGTTGGGA AAACAGACTT AGACACAGAG GGAGGTATCA CCTTGCCTTG TATTATTCCT
    GGACCGTATG GTCCATCAGG ACTCCCAGGA GCTCCCGGAT TCCCAGGCCC TAAAGGGGCC
    CGTGGCCTCC CTGGGGCTCC AGGCCAACCT GGATTGCGTG GAAATAAAGG GGACCCCGGA
    AGTCCAGGAT TGGTTCACCT GCCTGAATTG CCAGGGTTTC CTGGACCTCA TGGAGAGAAG
    GGCTTACCTG GGTTTCCTGG GCTCCCTGGA AAAGACGGCT TGCCTGGGAA AGTTGGCAGC
    CCCGGCTTAC CAGGTCCCAA GGGAGCCCCT GGTGACATCC TTGGTGCTGA AAATGGTGCT
    CCGGGGGAGC AAGGCCTACA GGGACTGCCT GGGGACAGAG GATTTCCTGG AGACTCTGGC
    CTTCCAGGAC TCAAGGGTTT GCTTGGGAAG TCGGGCTTGC TCGGCCCCAA AGGTGAGCGG
    GGCAGCCCTG GAATACCAGG CCGTGTGGGT CAGCCAGGAC CCCCAGGCCC TAGTGGTCTA
    TTTGGCATCA AGGGCAAACC CGGGCTCCGC GGACCCCCAG GCTTTCCAGG CATTTCAGGA
    TATCCTGGAA AGAAAGGTCC AAGAGGCGAG ATAGGTCTCC CTGCGTCAGC TGGAAAGAGA
    GGCCTGCCTG GGCTGAAAGG GCTTCCGGGA CTGCCAGGGC CAATTGGCTT CCTGGGGAAC
    TCAGGCTTGC CAGGGGCCAC TGGGTTACCA GGCCTGCCAG GTCTGAAGGG TGAGAAGGGG
    TCTGTTGGAC TGACAGGTTT TCCAGGGATT CCAGGTCTCC CTGGTATTCC TGGAGCAAGT
    GGATTAAAGG GAATTCCTGG GTCGACAGGA AGAGTGGGGC CATCTGGATG GGCTGGTAGC
    CCTGGTGAAA AAGGAGACAG AGGTGACCCA GGCCCCTTTG GGATACCCAG TCATCGACCT
    CCAATGCTGA ACCTCCGCTT CAAAGGAGAC AAAGGTTCTC AGGGCTCACC TGGAGCCATT
    GGATTTCCTG GACCCAGAGG TGACAAAGGA GAGGCTGGCC ACCCCGGGCC ACCAGGCTTG
    CCTGGAGCTC CTGGCTTCCC CAGCCTCATC AAGGGACTTA GTGGGAGACC AGGCCCCCCT
    GGCTCCACAG GACTACGGGG CTTACCTGGC CTGAAGGGGT ACCCTGGAAT TGCAGGTTTC
    CAAGGAATTC CAGGAGAAAG TGGTTCACAG GGTCTCAGTG GAGTTCCCGG GCTACCAGGA
    GCATCTGGTC TCCCAGGTTT AAAAGGAGAT CAAGGCCAGA CACTTGGAAT TTCTGGTAGC
    CCAGGACCCA AGGGACAACC TGGGGAGTCT GGTTTCAAAG GTGTGAAAGG AAAAGACGGA
    CCAGTTGGTG ATGTGGGTTT CCCAGGAAAC AAAGGTGAAG ATGGGAAAGT TGGTATTTCT
    GGAGATGTTG GCCTTCCTGG CTCCCTAGGA CTCCCCGGAG TTGCAGGCAT GAGAGGAAAT
    CCAGGGCTTC CAGGTTCTCC AGGCCACCCA GGGGCAACTG GGCCCCTGGG TCCCCCTGGC
    CTAATAGGAA CCAAAGGTTT CCCTGGACTT CCAGGTTTAC ATGGACTGAA TGGGCTTCCA
    GGAACCAAGG GGACTCATGG AACTCCAGGA CCTAGTATAA CCGGTGTGCC TGGGCCAGCT
    GGCTTGCCTG GTCCCAAAGG GGAAAAAGGT TCTCCAGGAA TTGGCATCGG AGCCCCAGGA
    AAGCCAGGTA TAAGAGGACC CAAAGGTAGT CAAGGTTTTC CAGGTCTCCA GGGCGCCGCT
    GGTCTCCCCG GTGCACTGGG CCTCTCCTTG CCTTCAGTCA TAGAAGGACA GCCTGGTGAC
    CCTGGGCGAC CAGGTCTAGA TGGAGAACGA GGCCGCCCAG GCCCTCCTGG GCCCCCAGGC
    CCCCCTGGGC CTGCCTCAGA TCAAGGCGAC CCTGGAGACC CTGGCTTCCC TGGAATTCCT
    GGCCCTCAAG GGCCCAAAGG AGACCAAGGA ATTCCAGGTT TCTCTGGCCT CCCTGGAGAG
    CTAGGATTGA AAGGCATGAG AGGTGAGCCT GGCTTCATGG GGACTCCAGG CAAGGTTGGA
    CCACCTGGAG ACCCAGGACT TCCCGGGATG AAGGGGAAGG CGGGGCCAAG AGGCTTATCT
    GGCCCTCGAG GTGCTCCTGG ACACACACCC ATTGCAGAAG CCATCCAGGC TCCCCCTGGA
    CCCATGGGTC TTCCAGGCAT TGATGGAATC CCTGGCCTCA TGGGAGACCC TGGGGTCCAA
    GGACCTGTGG GCCTACAAGG ATCTAAAGGT TTACCTGGCA TCCCTGGCAA AGATGGCGCC
    AGTGGACTCC CTGGCCCACC TGGGGCTCTT GGTGATCCTG GTCTCCCTGG ACTACAAGGC
    CCTCCAGGAT TTGAAGGAGC TCCAGGGAAG CCGGGCCCCT TCGGGAGGGC TGGAGTGCCT
    GGACAGAGCC TGAGAGTGGG CTACACCTTG GTGAAGCACA GCCAGTCAGA ACAGGTGCCC
    CTGTGCCCCA GCGGGATGAG CCAGCTCTGG GTGGGTTACA GCCTGCTGTT CGTGGAGGGG
    CAAGAGAAAG CCCACAACCA GGACCTGGGG TTTGCTGGCT CCTGCCTGCC CCGCTTCAGC
    ACCATGCCAT TCATCTACTG CAACATCAAT GAAGTGTGCC ACTATGCCCG ACGGAATGAT
    AAGTCCTACT GGCTCTCCAC CACCGCCCCC ATCCCTATGA TGCCGGTCGG CCAGACACAG
    ATCCCCCAGT ACATCAGCCG CTGCTCTGTG TGTGAGGCGC CCTCGCAAGC CATTGCTGTG
    CACAGCCAGG ACATCACCAT CCCACAGTGC CCCCTGGGCT GGCGCAGTCT GTGGATTGGG
    TACTCCTTCC TCATGCACAC AGCCGCCGGT GCAGAGGGCG GGGGCCAGTC CCTGGTCTCT
    CCCGGATCCT GCCTAGAGGA TTTCCGGGCT ACTCCTTTCA TCGAATGCAG TGGTGCCCGG
    GGCACCTGCC ACTACTTTGC CAACAAATAT AGTTTCTGGC TGACAACAGT GGAAGAGAGG
    CAGCAGTTTG GGGAGGAGCC TGTATCTGAG ACGCTGAAAG CTGGACAGCT CCACACCCGA
    GTCAGCCGCT GCCAGGTGTG TATGAAAAGC GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844573.1
    CDS256..5331
    Translation

    Target ORF information:

    RefSeq Version XM_024988805.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 6 chain (COL4A6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988805.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
    GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT
    AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA
    ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC
    TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGCCACCC TGGACAGCCA
    GGACCCAGAG GCCCACCTGG CCTGGATGGC TGTAATGGAA CTCAAGGAGC TGTTGGATTT
    CCAGGCCCTG ATGGCTATTC TGGGCTTCCT GGACCACCTG GGCTTCCTGG TCAGAAAGGC
    TCTAAAGGTG AACCTGTCCT TGCCCAAGGT AGTTTCAAAG GAATGAAGGG GGAGTCTGGG
    CTGCCCGGAC TGGATGGAAT CACTGGCCCA CAAGGAGCAC CCGGATCTCC AGGAGCTGTA
    GGACCCAGAG GACCACCAGG ATTGCAAGGT CCTCCTGGCC CCCCTGGTTT CCCTGGTCCT
    GATGGGAATA TGGGGTTAGG TTTCCAAGGA GAGAAAGGAG TCAAGGGAGA TGTTGGCCTG
    CCTGGTCCTG CAGGACCCCC ACCATCTACT GGAGAACTGG AATTCATGGG ATTTCCCAAA
    GGGAAGAAAG GATCCAAGGG TGAACCAGGG CCTCAGGGCT TCCCAGGAGT AAGTGGTCCA
    CCTGGTCTGC CGGGATTTGG AATTGCTGGA GAAAAAGGAG AAAAGGGAAT TCCTGGTTTG
    CCAGGACCTA GGGGTCCCAT GGGTTTAGAA GGAGTCCAAG GCCCTCCGGG GAGTCAGGGC
    AGGAAGGGGT CTTCAGGTTT CCCCGGGCTT AATGGATTCC CAGGAATTAA GGGTGAAAAA
    GGTGGCATTG GCCTGCCAGG CCCAGATATT TTCATCGATA CAGATGGTGC TGTGATCTCA
    GGTTATCCTG GAGACCCTGG TATGCCAGGC CTCCCAGGCC TTAAAGGAGA TGAAGGCATA
    CAGGGCCTAC CTGGCCCTCC TGGCGCCCCT GGATTACCAG CTTTACCAGG TGGCCCCGGT
    GCTCTGGGGC CCCAGGGAGC TCTAGGCCTG AAGGGGGAAC GAGGAAACAC TGGCCGGACC
    ACAACGGGGG CAGCTGGTCT CCCTGGCAGA GATGGTTTGC CAGGCCTGCC AGGCCTACCA
    GGCCCACCTG GTGCAGCATC TGAGACGGGA ACCCTACAGA GCACAGAACC GGGATCGCCG
    GGACTCCGAG GGGAACGAGG TCCAAAAGGA CACCCAGGCC TCAAAGGAAT TAAAGGGGAC
    CCAGGTTTTT GTGCTTGTGA TGGTGGTGTC CCCAACATGG GGCCACCTGG GGAGCCAGGC
    CTGCCTGGGC CACCAGGACT TCTGGGCCTT CCAGGTCTTA AAGGAACCAA AGGTGATCCA
    GGCTCTGGGG GTGCACAGGG CCCATCAGGG GATCCAGGTG TATTTGGGCC TCGAGGTCCA
    ACAGGCCCTA AAGGAAAGAA AGGGGACCCA ACTCTCAGCA CAAGATCAGG GATGCCAGGA
    GAGCAAGGTG ATCCTGGCCC CCAGGGGCTC CCTGGTGAGA CAGGAGCCCC AGGCAAGGAT
    GGAATACCAG GTTTACCAGG GCTGCCAGGC CTTCTGGGTG ATGGTGCACA GGGCTTCCCC
    GGTGAAAAGG GGTTACCAGG ACTTCCTGGT GAAAAAGGCC ACACTGGTCC AACTGGCCCC
    CCAGGAATTG GGCTACCAGG GGCTCCTGGA CCTCGTGGAC TTCCTGGAGA TAAAGGAGTA
    GATGGATTAC CAGGGCAACC AGGCCCCCGT GGAGTTCCAG GTGACTGCTG CTGCAAGGAG
    GAGGTTGGGA AAACAGACTT AGACACAGAG GGAGGTATCA CCTTGCCTTG TATTATTCCT
    GGACCGTATG GTCCATCAGG ACTCCCAGGA GCTCCCGGAT TCCCAGGCCC TAAAGGGGCC
    CGTGGCCTCC CTGGGGCTCC AGGCCAACCT GGATTGCGTG GAAATAAAGG GGACCCCGGA
    AGTCCAGGAT TGGTTCACCT GCCTGAATTG CCAGGGTTTC CTGGACCTCA TGGAGAGAAG
    GGCTTACCTG GGTTTCCTGG GCTCCCTGGA AAAGACGGCT TGCCTGGGAA AGTTGGCAGC
    CCCGGCTTAC CAGGTCCCAA GGGAGCCCCT GGTGACATCC TTGGTGCTGA AAATGGTGCT
    CCGGGGGAGC AAGGCCTACA GGGACTGCCT GGGGACAGAG GATTTCCTGG AGACTCTGGC
    CTTCCAGGAC TCAAGGGTTT GCTTGGGAAG TCGGGCTTGC TCGGCCCCAA AGGTGAGCGG
    GGCAGCCCTG GAATACCAGG CCGTGTGGGT CAGCCAGGAC CCCCAGGCCC TAGTGGTCTA
    TTTGGCATCA AGGGCAAACC CGGGCTCCGC GGACCCCCAG GCTTTCCAGG CATTTCAGGA
    TATCCTGGAA AGAAAGGTCC AAGAGGCGAG ATAGGTCTCC CTGCGTCAGC TGGAAAGAGA
    GGCCTGCCTG GGCTGAAAGG GCTTCCGGGA CTGCCAGGGC CAATTGGCTT CCTGGGGAAC
    TCAGGCTTGC CAGGGGCCAC TGGGTTACCA GGCCTGCCAG GTCTGAAGGG TGAGAAGGGG
    TCTGTTGGAC TGACAGGTTT TCCAGGGATT CCAGGTCTCC CTGGTATTCC TGGAGCAAGT
    GGATTAAAGG GAATTCCTGG GTCGACAGGA AGAGTGGGGC CATCTGGATG GGCTGGTAGC
    CCTGGTGAAA AAGGAGACAG AGGTGACCCA GGCCCCTTTG GGATACCCAG TCATCGACCT
    CCAATGCTGA ACCTCCGCTT CAAAGGAGAC AAAGGTTCTC AGGGCTCACC TGGAGCCATT
    GGATTTCCTG GACCCAGAGG TGACAAAGGA GAGGCTGGCC ACCCCGGGCC ACCAGGCTTG
    CCTGGAGCTC CTGGCTTCCC CAGCCTCATC AAGGGACTTA GTGGGAGACC AGGCCCCCCT
    GGCTCCACAG GACTACGGGG CTTACCTGGC CTGAAGGGGT ACCCTGGAAT TGCAGGTTTC
    CAAGGAATTC CAGGAGAAAG TGGTTCACAG GGTCTCAGTG GAGTTCCCGG GCTACCAGGA
    GCATCTGGTC TCCCAGGTTT AAAAGGAGAT CAAGGCCAGA CACTTGGAAT TTCTGGTAGC
    CCAGGACCCA AGGGACAACC TGGGGAGTCT GGTTTCAAAG GTGTGAAAGG AAAAGACGGA
    CCAGTTGGTG ATGTGGGTTT CCCAGGAAAC AAAGGTGAAG ATGGGAAAGT TGGTATTTCT
    GGAGATGTTG GCCTTCCTGG CTCCCTAGGA CTCCCCGGAG TTGCAGGCAT GAGAGGAAAT
    CCAGGGCTTC CAGGTTCTCC AGGCCACCCA GGGGCAACTG GGCCCCTGGG TCCCCCTGGC
    CTAATAGGAA CCAAAGGTTT CCCTGGACTT CCAGGTTTAC ATGGACTGAA TGGGCTTCCA
    GGAACCAAGG GGACTCATGG AACTCCAGGA CCTAGTATAA CCGGTGTGCC TGGGCCAGCT
    GGCTTGCCTG GTCCCAAAGG GGAAAAAGGT TCTCCAGGAA TTGGCATCGG AGCCCCAGGA
    AAGCCAGGTA TAAGAGGACC CAAAGGTAGT CAAGGTTTTC CAGGTCTCCA GGGCGCCGCT
    GGTCTCCCCG GTGCACTGGG CCTCTCCTTG CCTTCAGTCA TAGAAGGACA GCCTGGTGAC
    CCTGGGCGAC CAGGTCTAGA TGGAGAACGA GGCCGCCCAG GCCCTCCTGG GCCCCCAGGC
    CCCCCTGGGC CTGCCTCAGA TCAAGGCGAC CCTGGAGACC CTGGCTTCCC TGGAATTCCT
    GGCCCTCAAG GGCCCAAAGG AGACCAAGGA ATTCCAGGTT TCTCTGGCCT CCCTGGAGAG
    CTAGGATTGA AAGGCATGAG AGGTGAGCCT GGCTTCATGG GGACTCCAGG CAAGGTTGGA
    CCACCTGGAG ACCCAGGACT TCCCGGGATG AAGGGGAAGG CGGGGCCAAG AGGCTTATCT
    GGCCCTCGAG GTGCTCCTGG ACACACACCC ATTGCAGAAG CCATCCAGGC TCCCCCTGGA
    CCCATGGGTC TTCCAGGCAT TGATGGAATC CCTGGCCTCA TGGGAGACCC TGGGGTCCAA
    GGACCTGTGG GCCTACAAGG ATCTAAAGGT TTACCTGGCA TCCCTGGCAA AGATGGCGCC
    AGTGGACTCC CTGGCCCACC TGGGGCTCTT GGTGATCCTG GTCTCCCTGG ACTACAAGGC
    CCTCCAGGAT TTGAAGGAGC TCCAGGGAAG CCGGGCCCCT TCGGGAGGGC TGGAGTGCCT
    GGACAGAGCC TGAGAGTGGG CTACACCTTG GTGAAGCACA GCCAGTCAGA ACAGGTGCCC
    CTGTGCCCCA GCGGGATGAG CCAGCTCTGG GTGGGTTACA GCCTGCTGTT CGTGGAGGGG
    CAAGAGAAAG CCCACAACCA GGACCTGGGG TTTGCTGGCT CCTGCCTGCC CCGCTTCAGC
    ACCATGCCAT TCATCTACTG CAACATCAAT GAAGTGTGCC ACTATGCCCG ACGGAATGAT
    AAGTCCTACT GGCTCTCCAC CACCGCCCCC ATCCCTATGA TGCCGGTCGG CCAGACACAG
    ATCCCCCAGT ACATCAGCCG CTGCTCTGTG TGTGAGGCGC CCTCGCAAGC CATTGCTGTG
    CACAGCCAGG ACATCACCAT CCCACAGTGC CCCCTGGGCT GGCGCAGTCT GTGGATTGGG
    TACTCCTTCC TCATGCACAC AGCCGCCGGT GCAGAGGGCG GGGGCCAGTC CCTGGTCTCT
    CCCGGATCCT GCCTAGAGGA TTTCCGGGCT ACTCCTTTCA TCGAATGCAG TGGTGCCCGG
    GGCACCTGCC ACTACTTTGC CAACAAATAT AGTTTCTGGC TGACAACAGT GGAAGAGAGG
    CAGCAGTTTG GGGAGGAGCC TGTATCTGAG ACGCTGAAAG CTGGACAGCT CCACACCCGA
    GTCAGCCGCT GCCAGGTGTG TATGAAAAGC GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12979
    Clone ID Related Accession (Same CDS sequence) XM_005227897.3 , XM_601826.8 , XM_005227897.4
    Accession Version XM_005227897.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5121bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-6(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104715.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227897.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
    GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT
    AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA
    ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC
    TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC
    GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA
    CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC
    TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT
    GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA
    CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA
    CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA
    TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT
    CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA
    GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG
    CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC
    CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC
    CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG
    GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT
    GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA
    GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA
    CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT
    TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA
    GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA
    AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA
    CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA
    CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA
    CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG
    CAACCAGGCC CCCGTGGAGT TCCAGGTGAC TGCTGCTGCA AGGAGGAGGT TGGGAAAACA
    GACTTAGACA CAGAGGGAGG TATCACCTTG CCTTGTATTA TTCCTGGACC GTATGGTCCA
    TCAGGACTCC CAGGAGCTCC CGGATTCCCA GGCCCTAAAG GGGCCCGTGG CCTCCCTGGG
    GCTCCAGGCC AACCTGGATT GCGTGGAAAT AAAGGGGACC CCGGAAGTCC AGGATTGGTT
    CACCTGCCTG AATTGCCAGG GTTTCCTGGA CCTCATGGAG AGAAGGGCTT ACCTGGGTTT
    CCTGGGCTCC CTGGAAAAGA CGGCTTGCCT GGGAAAGTTG GCAGCCCCGG CTTACCAGGT
    CCCAAGGGAG CCCCTGGTGA CATCCTTGGT GCTGAAAATG GTGCTCCGGG GGAGCAAGGC
    CTACAGGGAC TGCCTGGGGA CAGAGGATTT CCTGGAGACT CTGGCCTTCC AGGACTCAAG
    GGTTTGCTTG GGAAGTCGGG CTTGCTCGGC CCCAAAGGTG AGCGGGGCAG CCCTGGAATA
    CCAGGCCGTG TGGGTCAGCC AGGACCCCCA GGCCCTAGTG GTCTATTTGG CATCAAGGGC
    AAACCCGGGC TCCGCGGACC CCCAGGCTTT CCAGGCATTT CAGGATATCC TGGAAAGAAA
    GGTCCAAGAG GCGAGATAGG TCTCCCTGCG TCAGCTGGAA AGAGAGGCCT GCCTGGGCTG
    AAAGGGCTTC CGGGACTGCC AGGGCCAATT GGCTTCCTGG GGAACTCAGG CTTGCCAGGG
    GCCACTGGGT TACCAGGCCT GCCAGGTCTG AAGGGTGAGA AGGGGTCTGT TGGACTGACA
    GGTTTTCCAG GGATTCCAGG TCTCCCTGGT ATTCCTGGAG CAAGTGGATT AAAGGGAATT
    CCTGGGTCGA CAGGAAGAGT GGGGCCATCT GGATGGGCTG GTAGCCCTGG TGAAAAAGGA
    GACAGAGGTG ACCCAGGCCC CTTTGGGATA CCCAGTCATC GACCTCCAAT GCTGAACCTC
    CGCTTCAAAG GAGACAAAGG TTCTCAGGGC TCACCTGGAG CCATTGGATT TCCTGGACCC
    AGAGGTGACA AAGGAGAGGC TGGCCACCCC GGGCCACCAG GCTTGCCTGG AGCTCCTGGC
    TTCCCCAGCC TCATCAAGGG ACTTAGTGGG AGACCAGGCC CCCCTGGCTC CACAGGACTA
    CGGGGCTTAC CTGGCCTGAA GGGGTACCCT GGAATTGCAG GTTTCCAAGG AATTCCAGGA
    GAAAGTGGTT CACAGGGTCT CAGTGGAGTT CCCGGGCTAC CAGGAGCATC TGGTCTCCCA
    GGTTTAAAAG GAGATCAAGG CCAGACACTT GGAATTTCTG GTAGCCCAGG ACCCAAGGGA
    CAACCTGGGG AGTCTGGTTT CAAAGGTGTG AAAGGAAAAG ACGGACCAGT TGGTGATGTG
    GGTTTCCCAG GAAACAAAGG TGAAGATGGG AAAGTTGGTA TTTCTGGAGA TGTTGGCCTT
    CCTGGCTCCC TAGGACTCCC CGGAGTTGCA GGCATGAGAG GAAATCCAGG GCTTCCAGGT
    TCTCCAGGCC ACCCAGGGGC AACTGGGCCC CTGGGTCCCC CTGGCCTAAT AGGAACCAAA
    GGTTTCCCTG GACTTCCAGG TTTACATGGA CTGAATGGGC TTCCAGGAAC CAAGGGGACT
    CATGGAACTC CAGGACCTAG TATAACCGGT GTGCCTGGGC CAGCTGGCTT GCCTGGTCCC
    AAAGGGGAAA AAGGTTCTCC AGGAATTGGC ATCGGAGCCC CAGGAAAGCC AGGTATAAGA
    GGACCCAAAG GTAGTCAAGG TTTTCCAGGT CTCCAGGGCG CCGCTGGTCT CCCCGGTGCA
    CTGGGCCTCT CCTTGCCTTC AGTCATAGAA GGACAGCCTG GTGACCCTGG GCGACCAGGT
    CTAGATGGAG AACGAGGCCG CCCAGGCCCT CCTGGGCCCC CAGGCCCCCC TGGGCCTGCC
    TCAGATCAAG GCGACCCTGG AGACCCTGGC TTCCCTGGAA TTCCTGGCCC TCAAGGGCCC
    AAAGGAGACC AAGGAATTCC AGGTTTCTCT GGCCTCCCTG GAGAGCTAGG ATTGAAAGGC
    ATGAGAGGTG AGCCTGGCTT CATGGGGACT CCAGGCAAGG TTGGACCACC TGGAGACCCA
    GGACTTCCCG GGATGAAGGG GAAGGCGGGG CCAAGAGGCT TATCTGGCCC TCGAGGTGCT
    CCTGGACACA CACCCATTGC AGAAGCCATC CAGGCTCCCC CTGGACCCAT GGGTCTTCCA
    GGCATTGATG GAATCCCTGG CCTCATGGGA GACCCTGGGG TCCAAGGACC TGTGGGCCTA
    CAAGGATCTA AAGGTTTACC TGGCATCCCT GGCAAAGATG GCGCCAGTGG ACTCCCTGGC
    CCACCTGGGG CTCTTGGTGA TCCTGGTCTC CCTGGACTAC AAGGCCCTCC AGGATTTGAA
    GGAGCTCCAG GGAAGCCGGG CCCCTTCGGG AGGGCTGGAG TGCCTGGACA GAGCCTGAGA
    GTGGGCTACA CCTTGGTGAA GCACAGCCAG TCAGAACAGG TGCCCCTGTG CCCCAGCGGG
    ATGAGCCAGC TCTGGGTGGG TTACAGCCTG CTGTTCGTGG AGGGGCAAGA GAAAGCCCAC
    AACCAGGACC TGGGGTTTGC TGGCTCCTGC CTGCCCCGCT TCAGCACCAT GCCATTCATC
    TACTGCAACA TCAATGAAGT GTGCCACTAT GCCCGACGGA ATGATAAGTC CTACTGGCTC
    TCCACCACCG CCCCCATCCC TATGATGCCG GTCGGCCAGA CACAGATCCC CCAGTACATC
    AGCCGCTGCT CTGTGTGTGA GGCGCCCTCG CAAGCCATTG CTGTGCACAG CCAGGACATC
    ACCATCCCAC AGTGCCCCCT GGGCTGGCGC AGTCTGTGGA TTGGGTACTC CTTCCTCATG
    CACACAGCCG CCGGTGCAGA GGGCGGGGGC CAGTCCCTGG TCTCTCCCGG ATCCTGCCTA
    GAGGATTTCC GGGCTACTCC TTTCATCGAA TGCAGTGGTG CCCGGGGCAC CTGCCACTAC
    TTTGCCAACA AATATAGTTT CTGGCTGACA ACAGTGGAAG AGAGGCAGCA GTTTGGGGAG
    GAGCCTGTAT CTGAGACGCT GAAAGCTGGA CAGCTCCACA CCCGAGTCAG CCGCTGCCAG
    GTGTGTATGA AAAGCGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005227954.1
    CDS270..5390
    Translation

    Target ORF information:

    RefSeq Version XM_005227897.3
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005227897.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
    GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT
    AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA
    ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC
    TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC
    GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA
    CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC
    TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT
    GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA
    CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA
    CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA
    TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT
    CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA
    GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG
    CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC
    CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC
    CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG
    GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT
    GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA
    GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA
    CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT
    TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA
    GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA
    AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA
    CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA
    CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA
    CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG
    CAACCAGGCC CCCGTGGAGT TCCAGGTGAC TGCTGCTGCA AGGAGGAGGT TGGGAAAACA
    GACTTAGACA CAGAGGGAGG TATCACCTTG CCTTGTATTA TTCCTGGACC GTATGGTCCA
    TCAGGACTCC CAGGAGCTCC CGGATTCCCA GGCCCTAAAG GGGCCCGTGG CCTCCCTGGG
    GCTCCAGGCC AACCTGGATT GCGTGGAAAT AAAGGGGACC CCGGAAGTCC AGGATTGGTT
    CACCTGCCTG AATTGCCAGG GTTTCCTGGA CCTCATGGAG AGAAGGGCTT ACCTGGGTTT
    CCTGGGCTCC CTGGAAAAGA CGGCTTGCCT GGGAAAGTTG GCAGCCCCGG CTTACCAGGT
    CCCAAGGGAG CCCCTGGTGA CATCCTTGGT GCTGAAAATG GTGCTCCGGG GGAGCAAGGC
    CTACAGGGAC TGCCTGGGGA CAGAGGATTT CCTGGAGACT CTGGCCTTCC AGGACTCAAG
    GGTTTGCTTG GGAAGTCGGG CTTGCTCGGC CCCAAAGGTG AGCGGGGCAG CCCTGGAATA
    CCAGGCCGTG TGGGTCAGCC AGGACCCCCA GGCCCTAGTG GTCTATTTGG CATCAAGGGC
    AAACCCGGGC TCCGCGGACC CCCAGGCTTT CCAGGCATTT CAGGATATCC TGGAAAGAAA
    GGTCCAAGAG GCGAGATAGG TCTCCCTGCG TCAGCTGGAA AGAGAGGCCT GCCTGGGCTG
    AAAGGGCTTC CGGGACTGCC AGGGCCAATT GGCTTCCTGG GGAACTCAGG CTTGCCAGGG
    GCCACTGGGT TACCAGGCCT GCCAGGTCTG AAGGGTGAGA AGGGGTCTGT TGGACTGACA
    GGTTTTCCAG GGATTCCAGG TCTCCCTGGT ATTCCTGGAG CAAGTGGATT AAAGGGAATT
    CCTGGGTCGA CAGGAAGAGT GGGGCCATCT GGATGGGCTG GTAGCCCTGG TGAAAAAGGA
    GACAGAGGTG ACCCAGGCCC CTTTGGGATA CCCAGTCATC GACCTCCAAT GCTGAACCTC
    CGCTTCAAAG GAGACAAAGG TTCTCAGGGC TCACCTGGAG CCATTGGATT TCCTGGACCC
    AGAGGTGACA AAGGAGAGGC TGGCCACCCC GGGCCACCAG GCTTGCCTGG AGCTCCTGGC
    TTCCCCAGCC TCATCAAGGG ACTTAGTGGG AGACCAGGCC CCCCTGGCTC CACAGGACTA
    CGGGGCTTAC CTGGCCTGAA GGGGTACCCT GGAATTGCAG GTTTCCAAGG AATTCCAGGA
    GAAAGTGGTT CACAGGGTCT CAGTGGAGTT CCCGGGCTAC CAGGAGCATC TGGTCTCCCA
    GGTTTAAAAG GAGATCAAGG CCAGACACTT GGAATTTCTG GTAGCCCAGG ACCCAAGGGA
    CAACCTGGGG AGTCTGGTTT CAAAGGTGTG AAAGGAAAAG ACGGACCAGT TGGTGATGTG
    GGTTTCCCAG GAAACAAAGG TGAAGATGGG AAAGTTGGTA TTTCTGGAGA TGTTGGCCTT
    CCTGGCTCCC TAGGACTCCC CGGAGTTGCA GGCATGAGAG GAAATCCAGG GCTTCCAGGT
    TCTCCAGGCC ACCCAGGGGC AACTGGGCCC CTGGGTCCCC CTGGCCTAAT AGGAACCAAA
    GGTTTCCCTG GACTTCCAGG TTTACATGGA CTGAATGGGC TTCCAGGAAC CAAGGGGACT
    CATGGAACTC CAGGACCTAG TATAACCGGT GTGCCTGGGC CAGCTGGCTT GCCTGGTCCC
    AAAGGGGAAA AAGGTTCTCC AGGAATTGGC ATCGGAGCCC CAGGAAAGCC AGGTATAAGA
    GGACCCAAAG GTAGTCAAGG TTTTCCAGGT CTCCAGGGCG CCGCTGGTCT CCCCGGTGCA
    CTGGGCCTCT CCTTGCCTTC AGTCATAGAA GGACAGCCTG GTGACCCTGG GCGACCAGGT
    CTAGATGGAG AACGAGGCCG CCCAGGCCCT CCTGGGCCCC CAGGCCCCCC TGGGCCTGCC
    TCAGATCAAG GCGACCCTGG AGACCCTGGC TTCCCTGGAA TTCCTGGCCC TCAAGGGCCC
    AAAGGAGACC AAGGAATTCC AGGTTTCTCT GGCCTCCCTG GAGAGCTAGG ATTGAAAGGC
    ATGAGAGGTG AGCCTGGCTT CATGGGGACT CCAGGCAAGG TTGGACCACC TGGAGACCCA
    GGACTTCCCG GGATGAAGGG GAAGGCGGGG CCAAGAGGCT TATCTGGCCC TCGAGGTGCT
    CCTGGACACA CACCCATTGC AGAAGCCATC CAGGCTCCCC CTGGACCCAT GGGTCTTCCA
    GGCATTGATG GAATCCCTGG CCTCATGGGA GACCCTGGGG TCCAAGGACC TGTGGGCCTA
    CAAGGATCTA AAGGTTTACC TGGCATCCCT GGCAAAGATG GCGCCAGTGG ACTCCCTGGC
    CCACCTGGGG CTCTTGGTGA TCCTGGTCTC CCTGGACTAC AAGGCCCTCC AGGATTTGAA
    GGAGCTCCAG GGAAGCCGGG CCCCTTCGGG AGGGCTGGAG TGCCTGGACA GAGCCTGAGA
    GTGGGCTACA CCTTGGTGAA GCACAGCCAG TCAGAACAGG TGCCCCTGTG CCCCAGCGGG
    ATGAGCCAGC TCTGGGTGGG TTACAGCCTG CTGTTCGTGG AGGGGCAAGA GAAAGCCCAC
    AACCAGGACC TGGGGTTTGC TGGCTCCTGC CTGCCCCGCT TCAGCACCAT GCCATTCATC
    TACTGCAACA TCAATGAAGT GTGCCACTAT GCCCGACGGA ATGATAAGTC CTACTGGCTC
    TCCACCACCG CCCCCATCCC TATGATGCCG GTCGGCCAGA CACAGATCCC CCAGTACATC
    AGCCGCTGCT CTGTGTGTGA GGCGCCCTCG CAAGCCATTG CTGTGCACAG CCAGGACATC
    ACCATCCCAC AGTGCCCCCT GGGCTGGCGC AGTCTGTGGA TTGGGTACTC CTTCCTCATG
    CACACAGCCG CCGGTGCAGA GGGCGGGGGC CAGTCCCTGG TCTCTCCCGG ATCCTGCCTA
    GAGGATTTCC GGGCTACTCC TTTCATCGAA TGCAGTGGTG CCCGGGGCAC CTGCCACTAC
    TTTGCCAACA AATATAGTTT CTGGCTGACA ACAGTGGAAG AGAGGCAGCA GTTTGGGGAG
    GAGCCTGTAT CTGAGACGCT GAAAGCTGGA CAGCTCCACA CCCGAGTCAG CCGCTGCCAG
    GTGTGTATGA AAAGCGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12979
    Clone ID Related Accession (Same CDS sequence) XM_005227897.3 , XM_601826.8 , XM_005227897.4
    Accession Version XM_601826.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5121bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-6(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_601826.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
    GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT
    AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA
    ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC
    TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC
    GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA
    CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC
    TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT
    GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA
    CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA
    CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA
    TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT
    CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA
    GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG
    CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC
    CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC
    CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG
    GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT
    GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA
    GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA
    CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT
    TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA
    GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA
    AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA
    CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA
    CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA
    CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG
    CAACCAGGCC CCCGTGGAGT TCCAGGTGAC TGCTGCTGCA AGGAGGAGGT TGGGAAAACA
    GACTTAGACA CAGAGGGAGG TATCACCTTG CCTTGTATTA TTCCTGGACC GTATGGTCCA
    TCAGGACTCC CAGGAGCTCC CGGATTCCCA GGCCCTAAAG GGGCCCGTGG CCTCCCTGGG
    GCTCCAGGCC AACCTGGATT GCGTGGAAAT AAAGGGGACC CCGGAAGTCC AGGATTGGTT
    CACCTGCCTG AATTGCCAGG GTTTCCTGGA CCTCATGGAG AGAAGGGCTT ACCTGGGTTT
    CCTGGGCTCC CTGGAAAAGA CGGCTTGCCT GGGAAAGTTG GCAGCCCCGG CTTACCAGGT
    CCCAAGGGAG CCCCTGGTGA CATCCTTGGT GCTGAAAATG GTGCTCCGGG GGAGCAAGGC
    CTACAGGGAC TGCCTGGGGA CAGAGGATTT CCTGGAGACT CTGGCCTTCC AGGACTCAAG
    GGTTTGCTTG GGAAGTCGGG CTTGCTCGGC CCCAAAGGTG AGCGGGGCAG CCCTGGAATA
    CCAGGCCGTG TGGGTCAGCC AGGACCCCCA GGCCCTAGTG GTCTATTTGG CATCAAGGGC
    AAACCCGGGC TCCGCGGACC CCCAGGCTTT CCAGGCATTT CAGGATATCC TGGAAAGAAA
    GGTCCAAGAG GCGAGATAGG TCTCCCTGCG TCAGCTGGAA AGAGAGGCCT GCCTGGGCTG
    AAAGGGCTTC CGGGACTGCC AGGGCCAATT GGCTTCCTGG GGAACTCAGG CTTGCCAGGG
    GCCACTGGGT TACCAGGCCT GCCAGGTCTG AAGGGTGAGA AGGGGTCTGT TGGACTGACA
    GGTTTTCCAG GGATTCCAGG TCTCCCTGGT ATTCCTGGAG CAAGTGGATT AAAGGGAATT
    CCTGGGTCGA CAGGAAGAGT GGGGCCATCT GGATGGGCTG GTAGCCCTGG TGAAAAAGGA
    GACAGAGGTG ACCCAGGCCC CTTTGGGATA CCCAGTCATC GACCTCCAAT GCTGAACCTC
    CGCTTCAAAG GAGACAAAGG TTCTCAGGGC TCACCTGGAG CCATTGGATT TCCTGGACCC
    AGAGGTGACA AAGGAGAGGC TGGCCACCCC GGGCCACCAG GCTTGCCTGG AGCTCCTGGC
    TTCCCCAGCC TCATCAAGGG ACTTAGTGGG AGACCAGGCC CCCCTGGCTC CACAGGACTA
    CGGGGCTTAC CTGGCCTGAA GGGGTACCCT GGAATTGCAG GTTTCCAAGG AATTCCAGGA
    GAAAGTGGTT CACAGGGTCT CAGTGGAGTT CCCGGGCTAC CAGGAGCATC TGGTCTCCCA
    GGTTTAAAAG GAGATCAAGG CCAGACACTT GGAATTTCTG GTAGCCCAGG ACCCAAGGGA
    CAACCTGGGG AGTCTGGTTT CAAAGGTGTG AAAGGAAAAG ACGGACCAGT TGGTGATGTG
    GGTTTCCCAG GAAACAAAGG TGAAGATGGG AAAGTTGGTA TTTCTGGAGA TGTTGGCCTT
    CCTGGCTCCC TAGGACTCCC CGGAGTTGCA GGCATGAGAG GAAATCCAGG GCTTCCAGGT
    TCTCCAGGCC ACCCAGGGGC AACTGGGCCC CTGGGTCCCC CTGGCCTAAT AGGAACCAAA
    GGTTTCCCTG GACTTCCAGG TTTACATGGA CTGAATGGGC TTCCAGGAAC CAAGGGGACT
    CATGGAACTC CAGGACCTAG TATAACCGGT GTGCCTGGGC CAGCTGGCTT GCCTGGTCCC
    AAAGGGGAAA AAGGTTCTCC AGGAATTGGC ATCGGAGCCC CAGGAAAGCC AGGTATAAGA
    GGACCCAAAG GTAGTCAAGG TTTTCCAGGT CTCCAGGGCG CCGCTGGTCT CCCCGGTGCA
    CTGGGCCTCT CCTTGCCTTC AGTCATAGAA GGACAGCCTG GTGACCCTGG GCGACCAGGT
    CTAGATGGAG AACGAGGCCG CCCAGGCCCT CCTGGGCCCC CAGGCCCCCC TGGGCCTGCC
    TCAGATCAAG GCGACCCTGG AGACCCTGGC TTCCCTGGAA TTCCTGGCCC TCAAGGGCCC
    AAAGGAGACC AAGGAATTCC AGGTTTCTCT GGCCTCCCTG GAGAGCTAGG ATTGAAAGGC
    ATGAGAGGTG AGCCTGGCTT CATGGGGACT CCAGGCAAGG TTGGACCACC TGGAGACCCA
    GGACTTCCCG GGATGAAGGG GAAGGCGGGG CCAAGAGGCT TATCTGGCCC TCGAGGTGCT
    CCTGGACACA CACCCATTGC AGAAGCCATC CAGGCTCCCC CTGGACCCAT GGGTCTTCCA
    GGCATTGATG GAATCCCTGG CCTCATGGGA GACCCTGGGG TCCAAGGACC TGTGGGCCTA
    CAAGGATCTA AAGGTTTACC TGGCATCCCT GGCAAAGATG GCGCCAGTGG ACTCCCTGGC
    CCACCTGGGG CTCTTGGTGA TCCTGGTCTC CCTGGACTAC AAGGCCCTCC AGGATTTGAA
    GGAGCTCCAG GGAAGCCGGG CCCCTTCGGG AGGGCTGGAG TGCCTGGACA GAGCCTGAGA
    GTGGGCTACA CCTTGGTGAA GCACAGCCAG TCAGAACAGG TGCCCCTGTG CCCCAGCGGG
    ATGAGCCAGC TCTGGGTGGG TTACAGCCTG CTGTTCGTGG AGGGGCAAGA GAAAGCCCAC
    AACCAGGACC TGGGGTTTGC TGGCTCCTGC CTGCCCCGCT TCAGCACCAT GCCATTCATC
    TACTGCAACA TCAATGAAGT GTGCCACTAT GCCCGACGGA ATGATAAGTC CTACTGGCTC
    TCCACCACCG CCCCCATCCC TATGATGCCG GTCGGCCAGA CACAGATCCC CCAGTACATC
    AGCCGCTGCT CTGTGTGTGA GGCGCCCTCG CAAGCCATTG CTGTGCACAG CCAGGACATC
    ACCATCCCAC AGTGCCCCCT GGGCTGGCGC AGTCTGTGGA TTGGGTACTC CTTCCTCATG
    CACACAGCCG CCGGTGCAGA GGGCGGGGGC CAGTCCCTGG TCTCTCCCGG ATCCTGCCTA
    GAGGATTTCC GGGCTACTCC TTTCATCGAA TGCAGTGGTG CCCGGGGCAC CTGCCACTAC
    TTTGCCAACA AATATAGTTT CTGGCTGACA ACAGTGGAAG AGAGGCAGCA GTTTGGGGAG
    GAGCCTGTAT CTGAGACGCT GAAAGCTGGA CAGCTCCACA CCCGAGTCAG CCGCTGCCAG
    GTGTGTATGA AAAGCGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_601826.4
    CDS270..5390
    Translation

    Target ORF information:

    RefSeq Version XM_601826.8
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_601826.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
    GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT
    AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA
    ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC
    TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC
    GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA
    CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC
    TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT
    GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA
    CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA
    CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA
    TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT
    CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA
    GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG
    CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC
    CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC
    CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG
    GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT
    GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA
    GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA
    CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT
    TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA
    GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA
    AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA
    CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA
    CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA
    CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG
    CAACCAGGCC CCCGTGGAGT TCCAGGTGAC TGCTGCTGCA AGGAGGAGGT TGGGAAAACA
    GACTTAGACA CAGAGGGAGG TATCACCTTG CCTTGTATTA TTCCTGGACC GTATGGTCCA
    TCAGGACTCC CAGGAGCTCC CGGATTCCCA GGCCCTAAAG GGGCCCGTGG CCTCCCTGGG
    GCTCCAGGCC AACCTGGATT GCGTGGAAAT AAAGGGGACC CCGGAAGTCC AGGATTGGTT
    CACCTGCCTG AATTGCCAGG GTTTCCTGGA CCTCATGGAG AGAAGGGCTT ACCTGGGTTT
    CCTGGGCTCC CTGGAAAAGA CGGCTTGCCT GGGAAAGTTG GCAGCCCCGG CTTACCAGGT
    CCCAAGGGAG CCCCTGGTGA CATCCTTGGT GCTGAAAATG GTGCTCCGGG GGAGCAAGGC
    CTACAGGGAC TGCCTGGGGA CAGAGGATTT CCTGGAGACT CTGGCCTTCC AGGACTCAAG
    GGTTTGCTTG GGAAGTCGGG CTTGCTCGGC CCCAAAGGTG AGCGGGGCAG CCCTGGAATA
    CCAGGCCGTG TGGGTCAGCC AGGACCCCCA GGCCCTAGTG GTCTATTTGG CATCAAGGGC
    AAACCCGGGC TCCGCGGACC CCCAGGCTTT CCAGGCATTT CAGGATATCC TGGAAAGAAA
    GGTCCAAGAG GCGAGATAGG TCTCCCTGCG TCAGCTGGAA AGAGAGGCCT GCCTGGGCTG
    AAAGGGCTTC CGGGACTGCC AGGGCCAATT GGCTTCCTGG GGAACTCAGG CTTGCCAGGG
    GCCACTGGGT TACCAGGCCT GCCAGGTCTG AAGGGTGAGA AGGGGTCTGT TGGACTGACA
    GGTTTTCCAG GGATTCCAGG TCTCCCTGGT ATTCCTGGAG CAAGTGGATT AAAGGGAATT
    CCTGGGTCGA CAGGAAGAGT GGGGCCATCT GGATGGGCTG GTAGCCCTGG TGAAAAAGGA
    GACAGAGGTG ACCCAGGCCC CTTTGGGATA CCCAGTCATC GACCTCCAAT GCTGAACCTC
    CGCTTCAAAG GAGACAAAGG TTCTCAGGGC TCACCTGGAG CCATTGGATT TCCTGGACCC
    AGAGGTGACA AAGGAGAGGC TGGCCACCCC GGGCCACCAG GCTTGCCTGG AGCTCCTGGC
    TTCCCCAGCC TCATCAAGGG ACTTAGTGGG AGACCAGGCC CCCCTGGCTC CACAGGACTA
    CGGGGCTTAC CTGGCCTGAA GGGGTACCCT GGAATTGCAG GTTTCCAAGG AATTCCAGGA
    GAAAGTGGTT CACAGGGTCT CAGTGGAGTT CCCGGGCTAC CAGGAGCATC TGGTCTCCCA
    GGTTTAAAAG GAGATCAAGG CCAGACACTT GGAATTTCTG GTAGCCCAGG ACCCAAGGGA
    CAACCTGGGG AGTCTGGTTT CAAAGGTGTG AAAGGAAAAG ACGGACCAGT TGGTGATGTG
    GGTTTCCCAG GAAACAAAGG TGAAGATGGG AAAGTTGGTA TTTCTGGAGA TGTTGGCCTT
    CCTGGCTCCC TAGGACTCCC CGGAGTTGCA GGCATGAGAG GAAATCCAGG GCTTCCAGGT
    TCTCCAGGCC ACCCAGGGGC AACTGGGCCC CTGGGTCCCC CTGGCCTAAT AGGAACCAAA
    GGTTTCCCTG GACTTCCAGG TTTACATGGA CTGAATGGGC TTCCAGGAAC CAAGGGGACT
    CATGGAACTC CAGGACCTAG TATAACCGGT GTGCCTGGGC CAGCTGGCTT GCCTGGTCCC
    AAAGGGGAAA AAGGTTCTCC AGGAATTGGC ATCGGAGCCC CAGGAAAGCC AGGTATAAGA
    GGACCCAAAG GTAGTCAAGG TTTTCCAGGT CTCCAGGGCG CCGCTGGTCT CCCCGGTGCA
    CTGGGCCTCT CCTTGCCTTC AGTCATAGAA GGACAGCCTG GTGACCCTGG GCGACCAGGT
    CTAGATGGAG AACGAGGCCG CCCAGGCCCT CCTGGGCCCC CAGGCCCCCC TGGGCCTGCC
    TCAGATCAAG GCGACCCTGG AGACCCTGGC TTCCCTGGAA TTCCTGGCCC TCAAGGGCCC
    AAAGGAGACC AAGGAATTCC AGGTTTCTCT GGCCTCCCTG GAGAGCTAGG ATTGAAAGGC
    ATGAGAGGTG AGCCTGGCTT CATGGGGACT CCAGGCAAGG TTGGACCACC TGGAGACCCA
    GGACTTCCCG GGATGAAGGG GAAGGCGGGG CCAAGAGGCT TATCTGGCCC TCGAGGTGCT
    CCTGGACACA CACCCATTGC AGAAGCCATC CAGGCTCCCC CTGGACCCAT GGGTCTTCCA
    GGCATTGATG GAATCCCTGG CCTCATGGGA GACCCTGGGG TCCAAGGACC TGTGGGCCTA
    CAAGGATCTA AAGGTTTACC TGGCATCCCT GGCAAAGATG GCGCCAGTGG ACTCCCTGGC
    CCACCTGGGG CTCTTGGTGA TCCTGGTCTC CCTGGACTAC AAGGCCCTCC AGGATTTGAA
    GGAGCTCCAG GGAAGCCGGG CCCCTTCGGG AGGGCTGGAG TGCCTGGACA GAGCCTGAGA
    GTGGGCTACA CCTTGGTGAA GCACAGCCAG TCAGAACAGG TGCCCCTGTG CCCCAGCGGG
    ATGAGCCAGC TCTGGGTGGG TTACAGCCTG CTGTTCGTGG AGGGGCAAGA GAAAGCCCAC
    AACCAGGACC TGGGGTTTGC TGGCTCCTGC CTGCCCCGCT TCAGCACCAT GCCATTCATC
    TACTGCAACA TCAATGAAGT GTGCCACTAT GCCCGACGGA ATGATAAGTC CTACTGGCTC
    TCCACCACCG CCCCCATCCC TATGATGCCG GTCGGCCAGA CACAGATCCC CCAGTACATC
    AGCCGCTGCT CTGTGTGTGA GGCGCCCTCG CAAGCCATTG CTGTGCACAG CCAGGACATC
    ACCATCCCAC AGTGCCCCCT GGGCTGGCGC AGTCTGTGGA TTGGGTACTC CTTCCTCATG
    CACACAGCCG CCGGTGCAGA GGGCGGGGGC CAGTCCCTGG TCTCTCCCGG ATCCTGCCTA
    GAGGATTTCC GGGCTACTCC TTTCATCGAA TGCAGTGGTG CCCGGGGCAC CTGCCACTAC
    TTTGCCAACA AATATAGTTT CTGGCTGACA ACAGTGGAAG AGAGGCAGCA GTTTGGGGAG
    GAGCCTGTAT CTGAGACGCT GAAAGCTGGA CAGCTCCACA CCCGAGTCAG CCGCTGCCAG
    GTGTGTATGA AAAGCGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12979
    Clone ID Related Accession (Same CDS sequence) XM_005227897.3 , XM_601826.8 , XM_005227897.4
    Accession Version XM_005227897.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5121bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-6(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227897.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
    GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT
    AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA
    ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC
    TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC
    GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA
    CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC
    TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT
    GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA
    CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA
    CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA
    TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT
    CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA
    GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG
    CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC
    CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC
    CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG
    GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT
    GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA
    GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA
    CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT
    TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA
    GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA
    AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA
    CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA
    CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA
    CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG
    CAACCAGGCC CCCGTGGAGT TCCAGGTGAC TGCTGCTGCA AGGAGGAGGT TGGGAAAACA
    GACTTAGACA CAGAGGGAGG TATCACCTTG CCTTGTATTA TTCCTGGACC GTATGGTCCA
    TCAGGACTCC CAGGAGCTCC CGGATTCCCA GGCCCTAAAG GGGCCCGTGG CCTCCCTGGG
    GCTCCAGGCC AACCTGGATT GCGTGGAAAT AAAGGGGACC CCGGAAGTCC AGGATTGGTT
    CACCTGCCTG AATTGCCAGG GTTTCCTGGA CCTCATGGAG AGAAGGGCTT ACCTGGGTTT
    CCTGGGCTCC CTGGAAAAGA CGGCTTGCCT GGGAAAGTTG GCAGCCCCGG CTTACCAGGT
    CCCAAGGGAG CCCCTGGTGA CATCCTTGGT GCTGAAAATG GTGCTCCGGG GGAGCAAGGC
    CTACAGGGAC TGCCTGGGGA CAGAGGATTT CCTGGAGACT CTGGCCTTCC AGGACTCAAG
    GGTTTGCTTG GGAAGTCGGG CTTGCTCGGC CCCAAAGGTG AGCGGGGCAG CCCTGGAATA
    CCAGGCCGTG TGGGTCAGCC AGGACCCCCA GGCCCTAGTG GTCTATTTGG CATCAAGGGC
    AAACCCGGGC TCCGCGGACC CCCAGGCTTT CCAGGCATTT CAGGATATCC TGGAAAGAAA
    GGTCCAAGAG GCGAGATAGG TCTCCCTGCG TCAGCTGGAA AGAGAGGCCT GCCTGGGCTG
    AAAGGGCTTC CGGGACTGCC AGGGCCAATT GGCTTCCTGG GGAACTCAGG CTTGCCAGGG
    GCCACTGGGT TACCAGGCCT GCCAGGTCTG AAGGGTGAGA AGGGGTCTGT TGGACTGACA
    GGTTTTCCAG GGATTCCAGG TCTCCCTGGT ATTCCTGGAG CAAGTGGATT AAAGGGAATT
    CCTGGGTCGA CAGGAAGAGT GGGGCCATCT GGATGGGCTG GTAGCCCTGG TGAAAAAGGA
    GACAGAGGTG ACCCAGGCCC CTTTGGGATA CCCAGTCATC GACCTCCAAT GCTGAACCTC
    CGCTTCAAAG GAGACAAAGG TTCTCAGGGC TCACCTGGAG CCATTGGATT TCCTGGACCC
    AGAGGTGACA AAGGAGAGGC TGGCCACCCC GGGCCACCAG GCTTGCCTGG AGCTCCTGGC
    TTCCCCAGCC TCATCAAGGG ACTTAGTGGG AGACCAGGCC CCCCTGGCTC CACAGGACTA
    CGGGGCTTAC CTGGCCTGAA GGGGTACCCT GGAATTGCAG GTTTCCAAGG AATTCCAGGA
    GAAAGTGGTT CACAGGGTCT CAGTGGAGTT CCCGGGCTAC CAGGAGCATC TGGTCTCCCA
    GGTTTAAAAG GAGATCAAGG CCAGACACTT GGAATTTCTG GTAGCCCAGG ACCCAAGGGA
    CAACCTGGGG AGTCTGGTTT CAAAGGTGTG AAAGGAAAAG ACGGACCAGT TGGTGATGTG
    GGTTTCCCAG GAAACAAAGG TGAAGATGGG AAAGTTGGTA TTTCTGGAGA TGTTGGCCTT
    CCTGGCTCCC TAGGACTCCC CGGAGTTGCA GGCATGAGAG GAAATCCAGG GCTTCCAGGT
    TCTCCAGGCC ACCCAGGGGC AACTGGGCCC CTGGGTCCCC CTGGCCTAAT AGGAACCAAA
    GGTTTCCCTG GACTTCCAGG TTTACATGGA CTGAATGGGC TTCCAGGAAC CAAGGGGACT
    CATGGAACTC CAGGACCTAG TATAACCGGT GTGCCTGGGC CAGCTGGCTT GCCTGGTCCC
    AAAGGGGAAA AAGGTTCTCC AGGAATTGGC ATCGGAGCCC CAGGAAAGCC AGGTATAAGA
    GGACCCAAAG GTAGTCAAGG TTTTCCAGGT CTCCAGGGCG CCGCTGGTCT CCCCGGTGCA
    CTGGGCCTCT CCTTGCCTTC AGTCATAGAA GGACAGCCTG GTGACCCTGG GCGACCAGGT
    CTAGATGGAG AACGAGGCCG CCCAGGCCCT CCTGGGCCCC CAGGCCCCCC TGGGCCTGCC
    TCAGATCAAG GCGACCCTGG AGACCCTGGC TTCCCTGGAA TTCCTGGCCC TCAAGGGCCC
    AAAGGAGACC AAGGAATTCC AGGTTTCTCT GGCCTCCCTG GAGAGCTAGG ATTGAAAGGC
    ATGAGAGGTG AGCCTGGCTT CATGGGGACT CCAGGCAAGG TTGGACCACC TGGAGACCCA
    GGACTTCCCG GGATGAAGGG GAAGGCGGGG CCAAGAGGCT TATCTGGCCC TCGAGGTGCT
    CCTGGACACA CACCCATTGC AGAAGCCATC CAGGCTCCCC CTGGACCCAT GGGTCTTCCA
    GGCATTGATG GAATCCCTGG CCTCATGGGA GACCCTGGGG TCCAAGGACC TGTGGGCCTA
    CAAGGATCTA AAGGTTTACC TGGCATCCCT GGCAAAGATG GCGCCAGTGG ACTCCCTGGC
    CCACCTGGGG CTCTTGGTGA TCCTGGTCTC CCTGGACTAC AAGGCCCTCC AGGATTTGAA
    GGAGCTCCAG GGAAGCCGGG CCCCTTCGGG AGGGCTGGAG TGCCTGGACA GAGCCTGAGA
    GTGGGCTACA CCTTGGTGAA GCACAGCCAG TCAGAACAGG TGCCCCTGTG CCCCAGCGGG
    ATGAGCCAGC TCTGGGTGGG TTACAGCCTG CTGTTCGTGG AGGGGCAAGA GAAAGCCCAC
    AACCAGGACC TGGGGTTTGC TGGCTCCTGC CTGCCCCGCT TCAGCACCAT GCCATTCATC
    TACTGCAACA TCAATGAAGT GTGCCACTAT GCCCGACGGA ATGATAAGTC CTACTGGCTC
    TCCACCACCG CCCCCATCCC TATGATGCCG GTCGGCCAGA CACAGATCCC CCAGTACATC
    AGCCGCTGCT CTGTGTGTGA GGCGCCCTCG CAAGCCATTG CTGTGCACAG CCAGGACATC
    ACCATCCCAC AGTGCCCCCT GGGCTGGCGC AGTCTGTGGA TTGGGTACTC CTTCCTCATG
    CACACAGCCG CCGGTGCAGA GGGCGGGGGC CAGTCCCTGG TCTCTCCCGG ATCCTGCCTA
    GAGGATTTCC GGGCTACTCC TTTCATCGAA TGCAGTGGTG CCCGGGGCAC CTGCCACTAC
    TTTGCCAACA AATATAGTTT CTGGCTGACA ACAGTGGAAG AGAGGCAGCA GTTTGGGGAG
    GAGCCTGTAT CTGAGACGCT GAAAGCTGGA CAGCTCCACA CCCGAGTCAG CCGCTGCCAG
    GTGTGTATGA AAAGCGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005227954.1
    CDS223..5343
    Translation

    Target ORF information:

    RefSeq Version XM_005227897.4
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 6 chain (COL4A6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005227897.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
    GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT
    AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA
    ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC
    TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC
    GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA
    CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC
    TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT
    GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA
    CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA
    CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA
    TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT
    CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA
    GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG
    CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC
    CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC
    CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG
    GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT
    GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA
    GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA
    CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT
    TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA
    GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA
    AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA
    CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA
    CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA
    CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG
    CAACCAGGCC CCCGTGGAGT TCCAGGTGAC TGCTGCTGCA AGGAGGAGGT TGGGAAAACA
    GACTTAGACA CAGAGGGAGG TATCACCTTG CCTTGTATTA TTCCTGGACC GTATGGTCCA
    TCAGGACTCC CAGGAGCTCC CGGATTCCCA GGCCCTAAAG GGGCCCGTGG CCTCCCTGGG
    GCTCCAGGCC AACCTGGATT GCGTGGAAAT AAAGGGGACC CCGGAAGTCC AGGATTGGTT
    CACCTGCCTG AATTGCCAGG GTTTCCTGGA CCTCATGGAG AGAAGGGCTT ACCTGGGTTT
    CCTGGGCTCC CTGGAAAAGA CGGCTTGCCT GGGAAAGTTG GCAGCCCCGG CTTACCAGGT
    CCCAAGGGAG CCCCTGGTGA CATCCTTGGT GCTGAAAATG GTGCTCCGGG GGAGCAAGGC
    CTACAGGGAC TGCCTGGGGA CAGAGGATTT CCTGGAGACT CTGGCCTTCC AGGACTCAAG
    GGTTTGCTTG GGAAGTCGGG CTTGCTCGGC CCCAAAGGTG AGCGGGGCAG CCCTGGAATA
    CCAGGCCGTG TGGGTCAGCC AGGACCCCCA GGCCCTAGTG GTCTATTTGG CATCAAGGGC
    AAACCCGGGC TCCGCGGACC CCCAGGCTTT CCAGGCATTT CAGGATATCC TGGAAAGAAA
    GGTCCAAGAG GCGAGATAGG TCTCCCTGCG TCAGCTGGAA AGAGAGGCCT GCCTGGGCTG
    AAAGGGCTTC CGGGACTGCC AGGGCCAATT GGCTTCCTGG GGAACTCAGG CTTGCCAGGG
    GCCACTGGGT TACCAGGCCT GCCAGGTCTG AAGGGTGAGA AGGGGTCTGT TGGACTGACA
    GGTTTTCCAG GGATTCCAGG TCTCCCTGGT ATTCCTGGAG CAAGTGGATT AAAGGGAATT
    CCTGGGTCGA CAGGAAGAGT GGGGCCATCT GGATGGGCTG GTAGCCCTGG TGAAAAAGGA
    GACAGAGGTG ACCCAGGCCC CTTTGGGATA CCCAGTCATC GACCTCCAAT GCTGAACCTC
    CGCTTCAAAG GAGACAAAGG TTCTCAGGGC TCACCTGGAG CCATTGGATT TCCTGGACCC
    AGAGGTGACA AAGGAGAGGC TGGCCACCCC GGGCCACCAG GCTTGCCTGG AGCTCCTGGC
    TTCCCCAGCC TCATCAAGGG ACTTAGTGGG AGACCAGGCC CCCCTGGCTC CACAGGACTA
    CGGGGCTTAC CTGGCCTGAA GGGGTACCCT GGAATTGCAG GTTTCCAAGG AATTCCAGGA
    GAAAGTGGTT CACAGGGTCT CAGTGGAGTT CCCGGGCTAC CAGGAGCATC TGGTCTCCCA
    GGTTTAAAAG GAGATCAAGG CCAGACACTT GGAATTTCTG GTAGCCCAGG ACCCAAGGGA
    CAACCTGGGG AGTCTGGTTT CAAAGGTGTG AAAGGAAAAG ACGGACCAGT TGGTGATGTG
    GGTTTCCCAG GAAACAAAGG TGAAGATGGG AAAGTTGGTA TTTCTGGAGA TGTTGGCCTT
    CCTGGCTCCC TAGGACTCCC CGGAGTTGCA GGCATGAGAG GAAATCCAGG GCTTCCAGGT
    TCTCCAGGCC ACCCAGGGGC AACTGGGCCC CTGGGTCCCC CTGGCCTAAT AGGAACCAAA
    GGTTTCCCTG GACTTCCAGG TTTACATGGA CTGAATGGGC TTCCAGGAAC CAAGGGGACT
    CATGGAACTC CAGGACCTAG TATAACCGGT GTGCCTGGGC CAGCTGGCTT GCCTGGTCCC
    AAAGGGGAAA AAGGTTCTCC AGGAATTGGC ATCGGAGCCC CAGGAAAGCC AGGTATAAGA
    GGACCCAAAG GTAGTCAAGG TTTTCCAGGT CTCCAGGGCG CCGCTGGTCT CCCCGGTGCA
    CTGGGCCTCT CCTTGCCTTC AGTCATAGAA GGACAGCCTG GTGACCCTGG GCGACCAGGT
    CTAGATGGAG AACGAGGCCG CCCAGGCCCT CCTGGGCCCC CAGGCCCCCC TGGGCCTGCC
    TCAGATCAAG GCGACCCTGG AGACCCTGGC TTCCCTGGAA TTCCTGGCCC TCAAGGGCCC
    AAAGGAGACC AAGGAATTCC AGGTTTCTCT GGCCTCCCTG GAGAGCTAGG ATTGAAAGGC
    ATGAGAGGTG AGCCTGGCTT CATGGGGACT CCAGGCAAGG TTGGACCACC TGGAGACCCA
    GGACTTCCCG GGATGAAGGG GAAGGCGGGG CCAAGAGGCT TATCTGGCCC TCGAGGTGCT
    CCTGGACACA CACCCATTGC AGAAGCCATC CAGGCTCCCC CTGGACCCAT GGGTCTTCCA
    GGCATTGATG GAATCCCTGG CCTCATGGGA GACCCTGGGG TCCAAGGACC TGTGGGCCTA
    CAAGGATCTA AAGGTTTACC TGGCATCCCT GGCAAAGATG GCGCCAGTGG ACTCCCTGGC
    CCACCTGGGG CTCTTGGTGA TCCTGGTCTC CCTGGACTAC AAGGCCCTCC AGGATTTGAA
    GGAGCTCCAG GGAAGCCGGG CCCCTTCGGG AGGGCTGGAG TGCCTGGACA GAGCCTGAGA
    GTGGGCTACA CCTTGGTGAA GCACAGCCAG TCAGAACAGG TGCCCCTGTG CCCCAGCGGG
    ATGAGCCAGC TCTGGGTGGG TTACAGCCTG CTGTTCGTGG AGGGGCAAGA GAAAGCCCAC
    AACCAGGACC TGGGGTTTGC TGGCTCCTGC CTGCCCCGCT TCAGCACCAT GCCATTCATC
    TACTGCAACA TCAATGAAGT GTGCCACTAT GCCCGACGGA ATGATAAGTC CTACTGGCTC
    TCCACCACCG CCCCCATCCC TATGATGCCG GTCGGCCAGA CACAGATCCC CCAGTACATC
    AGCCGCTGCT CTGTGTGTGA GGCGCCCTCG CAAGCCATTG CTGTGCACAG CCAGGACATC
    ACCATCCCAC AGTGCCCCCT GGGCTGGCGC AGTCTGTGGA TTGGGTACTC CTTCCTCATG
    CACACAGCCG CCGGTGCAGA GGGCGGGGGC CAGTCCCTGG TCTCTCCCGG ATCCTGCCTA
    GAGGATTTCC GGGCTACTCC TTTCATCGAA TGCAGTGGTG CCCGGGGCAC CTGCCACTAC
    TTTGCCAACA AATATAGTTT CTGGCTGACA ACAGTGGAAG AGAGGCAGCA GTTTGGGGAG
    GAGCCTGTAT CTGAGACGCT GAAAGCTGGA CAGCTCCACA CCCGAGTCAG CCGCTGCCAG
    GTGTGTATGA AAAGCGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35386
    Clone ID Related Accession (Same CDS sequence) XM_010800459.2 , XM_002699886.5 , XM_002699886.4
    Accession Version XM_010800459.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-6(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010800459.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
    GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT
    AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA
    ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC
    TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC
    GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA
    CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC
    TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT
    GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA
    CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA
    CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA
    TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT
    CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA
    GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG
    CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC
    CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC
    CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG
    GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT
    GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA
    GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA
    CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT
    TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA
    GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA
    AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA
    CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA
    CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA
    CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG
    CAACCAGGCC CCCGTGGAGT TCCAGGTATC ACCTTGCCTT GTATTATTCC TGGACCGTAT
    GGTCCATCAG GACTCCCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC
    CCTGGGGCTC CAGGCCAACC TGGATTGCGT GGAAATAAAG GGGACCCCGG AAGTCCAGGA
    TTGGTTCACC TGCCTGAATT GCCAGGGTTT CCTGGACCTC ATGGAGAGAA GGGCTTACCT
    GGGTTTCCTG GGCTCCCTGG AAAAGACGGC TTGCCTGGGA AAGTTGGCAG CCCCGGCTTA
    CCAGGTCCCA AGGGAGCCCC TGGTGACATC CTTGGTGCTG AAAATGGTGC TCCGGGGGAG
    CAAGGCCTAC AGGGACTGCC TGGGGACAGA GGATTTCCTG GAGACTCTGG CCTTCCAGGA
    CTCAAGGGTT TGCTTGGGAA GTCGGGCTTG CTCGGCCCCA AAGGTGAGCG GGGCAGCCCT
    GGAATACCAG GCCGTGTGGG TCAGCCAGGA CCCCCAGGCC CTAGTGGTCT ATTTGGCATC
    AAGGGCAAAC CCGGGCTCCG CGGACCCCCA GGCTTTCCAG GCATTTCAGG ATATCCTGGA
    AAGAAAGGTC CAAGAGGCGA GATAGGTCTC CCTGCGTCAG CTGGAAAGAG AGGCCTGCCT
    GGGCTGAAAG GGCTTCCGGG ACTGCCAGGG CCAATTGGCT TCCTGGGGAA CTCAGGCTTG
    CCAGGGGCCA CTGGGTTACC AGGCCTGCCA GGTCTGAAGG GTGAGAAGGG GTCTGTTGGA
    CTGACAGGTT TTCCAGGGAT TCCAGGTCTC CCTGGTATTC CTGGAGCAAG TGGATTAAAG
    GGAATTCCTG GGTCGACAGG AAGAGTGGGG CCATCTGGAT GGGCTGGTAG CCCTGGTGAA
    AAAGGAGACA GAGGTGACCC AGGCCCCTTT GGGATACCCA GTCATCGACC TCCAATGCTG
    AACCTCCGCT TCAAAGGAGA CAAAGGTTCT CAGGGCTCAC CTGGAGCCAT TGGATTTCCT
    GGACCCAGAG GTGACAAAGG AGAGGCTGGC CACCCCGGGC CACCAGGCTT GCCTGGAGCT
    CCTGGCTTCC CCAGCCTCAT CAAGGGACTT AGTGGGAGAC CAGGCCCCCC TGGCTCCACA
    GGACTACGGG GCTTACCTGG CCTGAAGGGG TACCCTGGAA TTGCAGGTTT CCAAGGAATT
    CCAGGAGAAA GTGGTTCACA GGGTCTCAGT GGAGTTCCCG GGCTACCAGG AGCATCTGGT
    CTCCCAGGTT TAAAAGGAGA TCAAGGCCAG ACACTTGGAA TTTCTGGTAG CCCAGGACCC
    AAGGGACAAC CTGGGGAGTC TGGTTTCAAA GGTGTGAAAG GAAAAGACGG ACCAGTTGGT
    GATGTGGGTT TCCCAGGAAA CAAAGGTGAA GATGGGAAAG TTGGTATTTC TGGAGATGTT
    GGCCTTCCTG GCTCCCTAGG ACTCCCCGGA GTTGCAGGCA TGAGAGGAAA TCCAGGGCTT
    CCAGGTTCTC CAGGCCACCC AGGGGCAACT GGGCCCCTGG GTCCCCCTGG CCTAATAGGA
    ACCAAAGGTT TCCCTGGACT TCCAGGTTTA CATGGACTGA ATGGGCTTCC AGGAACCAAG
    GGGACTCATG GAACTCCAGG ACCTAGTATA ACCGGTGTGC CTGGGCCAGC TGGCTTGCCT
    GGTCCCAAAG GGGAAAAAGG TTCTCCAGGA ATTGGCATCG GAGCCCCAGG AAAGCCAGGT
    ATAAGAGGAC CCAAAGGTAG TCAAGGTTTT CCAGGTCTCC AGGGCGCCGC TGGTCTCCCC
    GGTGCACTGG GCCTCTCCTT GCCTTCAGTC ATAGAAGGAC AGCCTGGTGA CCCTGGGCGA
    CCAGGTCTAG ATGGAGAACG AGGCCGCCCA GGCCCTCCTG GGCCCCCAGG CCCCCCTGGG
    CCTGCCTCAG ATCAAGGCGA CCCTGGAGAC CCTGGCTTCC CTGGAATTCC TGGCCCTCAA
    GGGCCCAAAG GAGACCAAGG AATTCCAGGT TTCTCTGGCC TCCCTGGAGA GCTAGGATTG
    AAAGGCATGA GAGGTGAGCC TGGCTTCATG GGGACTCCAG GCAAGGTTGG ACCACCTGGA
    GACCCAGGAC TTCCCGGGAT GAAGGGGAAG GCGGGGCCAA GAGGCTTATC TGGCCCTCGA
    GGTGCTCCTG GACACACACC CATTGCAGAA GCCATCCAGG CTCCCCCTGG ACCCATGGGT
    CTTCCAGGCA TTGATGGAAT CCCTGGCCTC ATGGGAGACC CTGGGGTCCA AGGACCTGTG
    GGCCTACAAG GATCTAAAGG TTTACCTGGC ATCCCTGGCA AAGATGGCGC CAGTGGACTC
    CCTGGCCCAC CTGGGGCTCT TGGTGATCCT GGTCTCCCTG GACTACAAGG CCCTCCAGGA
    TTTGAAGGAG CTCCAGGGAA GCCGGGCCCC TTCGGGAGGG CTGGAGTGCC TGGACAGAGC
    CTGAGAGTGG GCTACACCTT GGTGAAGCAC AGCCAGTCAG AACAGGTGCC CCTGTGCCCC
    AGCGGGATGA GCCAGCTCTG GGTGGGTTAC AGCCTGCTGT TCGTGGAGGG GCAAGAGAAA
    GCCCACAACC AGGACCTGGG GTTTGCTGGC TCCTGCCTGC CCCGCTTCAG CACCATGCCA
    TTCATCTACT GCAACATCAA TGAAGTGTGC CACTATGCCC GACGGAATGA TAAGTCCTAC
    TGGCTCTCCA CCACCGCCCC CATCCCTATG ATGCCGGTCG GCCAGACACA GATCCCCCAG
    TACATCAGCC GCTGCTCTGT GTGTGAGGCG CCCTCGCAAG CCATTGCTGT GCACAGCCAG
    GACATCACCA TCCCACAGTG CCCCCTGGGC TGGCGCAGTC TGTGGATTGG GTACTCCTTC
    CTCATGCACA CAGCCGCCGG TGCAGAGGGC GGGGGCCAGT CCCTGGTCTC TCCCGGATCC
    TGCCTAGAGG ATTTCCGGGC TACTCCTTTC ATCGAATGCA GTGGTGCCCG GGGCACCTGC
    CACTACTTTG CCAACAAATA TAGTTTCTGG CTGACAACAG TGGAAGAGAG GCAGCAGTTT
    GGGGAGGAGC CTGTATCTGA GACGCTGAAA GCTGGACAGC TCCACACCCG AGTCAGCCGC
    TGCCAGGTGT GTATGAAAAG CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010798761.1
    CDS270..5336
    Translation

    Target ORF information:

    RefSeq Version XM_010800459.2
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010800459.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
    GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT
    AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA
    ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC
    TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC
    GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA
    CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC
    TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT
    GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA
    CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA
    CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA
    TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT
    CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA
    GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG
    CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC
    CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC
    CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG
    GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT
    GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA
    GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA
    CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT
    TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA
    GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA
    AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA
    CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA
    CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA
    CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG
    CAACCAGGCC CCCGTGGAGT TCCAGGTATC ACCTTGCCTT GTATTATTCC TGGACCGTAT
    GGTCCATCAG GACTCCCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC
    CCTGGGGCTC CAGGCCAACC TGGATTGCGT GGAAATAAAG GGGACCCCGG AAGTCCAGGA
    TTGGTTCACC TGCCTGAATT GCCAGGGTTT CCTGGACCTC ATGGAGAGAA GGGCTTACCT
    GGGTTTCCTG GGCTCCCTGG AAAAGACGGC TTGCCTGGGA AAGTTGGCAG CCCCGGCTTA
    CCAGGTCCCA AGGGAGCCCC TGGTGACATC CTTGGTGCTG AAAATGGTGC TCCGGGGGAG
    CAAGGCCTAC AGGGACTGCC TGGGGACAGA GGATTTCCTG GAGACTCTGG CCTTCCAGGA
    CTCAAGGGTT TGCTTGGGAA GTCGGGCTTG CTCGGCCCCA AAGGTGAGCG GGGCAGCCCT
    GGAATACCAG GCCGTGTGGG TCAGCCAGGA CCCCCAGGCC CTAGTGGTCT ATTTGGCATC
    AAGGGCAAAC CCGGGCTCCG CGGACCCCCA GGCTTTCCAG GCATTTCAGG ATATCCTGGA
    AAGAAAGGTC CAAGAGGCGA GATAGGTCTC CCTGCGTCAG CTGGAAAGAG AGGCCTGCCT
    GGGCTGAAAG GGCTTCCGGG ACTGCCAGGG CCAATTGGCT TCCTGGGGAA CTCAGGCTTG
    CCAGGGGCCA CTGGGTTACC AGGCCTGCCA GGTCTGAAGG GTGAGAAGGG GTCTGTTGGA
    CTGACAGGTT TTCCAGGGAT TCCAGGTCTC CCTGGTATTC CTGGAGCAAG TGGATTAAAG
    GGAATTCCTG GGTCGACAGG AAGAGTGGGG CCATCTGGAT GGGCTGGTAG CCCTGGTGAA
    AAAGGAGACA GAGGTGACCC AGGCCCCTTT GGGATACCCA GTCATCGACC TCCAATGCTG
    AACCTCCGCT TCAAAGGAGA CAAAGGTTCT CAGGGCTCAC CTGGAGCCAT TGGATTTCCT
    GGACCCAGAG GTGACAAAGG AGAGGCTGGC CACCCCGGGC CACCAGGCTT GCCTGGAGCT
    CCTGGCTTCC CCAGCCTCAT CAAGGGACTT AGTGGGAGAC CAGGCCCCCC TGGCTCCACA
    GGACTACGGG GCTTACCTGG CCTGAAGGGG TACCCTGGAA TTGCAGGTTT CCAAGGAATT
    CCAGGAGAAA GTGGTTCACA GGGTCTCAGT GGAGTTCCCG GGCTACCAGG AGCATCTGGT
    CTCCCAGGTT TAAAAGGAGA TCAAGGCCAG ACACTTGGAA TTTCTGGTAG CCCAGGACCC
    AAGGGACAAC CTGGGGAGTC TGGTTTCAAA GGTGTGAAAG GAAAAGACGG ACCAGTTGGT
    GATGTGGGTT TCCCAGGAAA CAAAGGTGAA GATGGGAAAG TTGGTATTTC TGGAGATGTT
    GGCCTTCCTG GCTCCCTAGG ACTCCCCGGA GTTGCAGGCA TGAGAGGAAA TCCAGGGCTT
    CCAGGTTCTC CAGGCCACCC AGGGGCAACT GGGCCCCTGG GTCCCCCTGG CCTAATAGGA
    ACCAAAGGTT TCCCTGGACT TCCAGGTTTA CATGGACTGA ATGGGCTTCC AGGAACCAAG
    GGGACTCATG GAACTCCAGG ACCTAGTATA ACCGGTGTGC CTGGGCCAGC TGGCTTGCCT
    GGTCCCAAAG GGGAAAAAGG TTCTCCAGGA ATTGGCATCG GAGCCCCAGG AAAGCCAGGT
    ATAAGAGGAC CCAAAGGTAG TCAAGGTTTT CCAGGTCTCC AGGGCGCCGC TGGTCTCCCC
    GGTGCACTGG GCCTCTCCTT GCCTTCAGTC ATAGAAGGAC AGCCTGGTGA CCCTGGGCGA
    CCAGGTCTAG ATGGAGAACG AGGCCGCCCA GGCCCTCCTG GGCCCCCAGG CCCCCCTGGG
    CCTGCCTCAG ATCAAGGCGA CCCTGGAGAC CCTGGCTTCC CTGGAATTCC TGGCCCTCAA
    GGGCCCAAAG GAGACCAAGG AATTCCAGGT TTCTCTGGCC TCCCTGGAGA GCTAGGATTG
    AAAGGCATGA GAGGTGAGCC TGGCTTCATG GGGACTCCAG GCAAGGTTGG ACCACCTGGA
    GACCCAGGAC TTCCCGGGAT GAAGGGGAAG GCGGGGCCAA GAGGCTTATC TGGCCCTCGA
    GGTGCTCCTG GACACACACC CATTGCAGAA GCCATCCAGG CTCCCCCTGG ACCCATGGGT
    CTTCCAGGCA TTGATGGAAT CCCTGGCCTC ATGGGAGACC CTGGGGTCCA AGGACCTGTG
    GGCCTACAAG GATCTAAAGG TTTACCTGGC ATCCCTGGCA AAGATGGCGC CAGTGGACTC
    CCTGGCCCAC CTGGGGCTCT TGGTGATCCT GGTCTCCCTG GACTACAAGG CCCTCCAGGA
    TTTGAAGGAG CTCCAGGGAA GCCGGGCCCC TTCGGGAGGG CTGGAGTGCC TGGACAGAGC
    CTGAGAGTGG GCTACACCTT GGTGAAGCAC AGCCAGTCAG AACAGGTGCC CCTGTGCCCC
    AGCGGGATGA GCCAGCTCTG GGTGGGTTAC AGCCTGCTGT TCGTGGAGGG GCAAGAGAAA
    GCCCACAACC AGGACCTGGG GTTTGCTGGC TCCTGCCTGC CCCGCTTCAG CACCATGCCA
    TTCATCTACT GCAACATCAA TGAAGTGTGC CACTATGCCC GACGGAATGA TAAGTCCTAC
    TGGCTCTCCA CCACCGCCCC CATCCCTATG ATGCCGGTCG GCCAGACACA GATCCCCCAG
    TACATCAGCC GCTGCTCTGT GTGTGAGGCG CCCTCGCAAG CCATTGCTGT GCACAGCCAG
    GACATCACCA TCCCACAGTG CCCCCTGGGC TGGCGCAGTC TGTGGATTGG GTACTCCTTC
    CTCATGCACA CAGCCGCCGG TGCAGAGGGC GGGGGCCAGT CCCTGGTCTC TCCCGGATCC
    TGCCTAGAGG ATTTCCGGGC TACTCCTTTC ATCGAATGCA GTGGTGCCCG GGGCACCTGC
    CACTACTTTG CCAACAAATA TAGTTTCTGG CTGACAACAG TGGAAGAGAG GCAGCAGTTT
    GGGGAGGAGC CTGTATCTGA GACGCTGAAA GCTGGACAGC TCCACACCCG AGTCAGCCGC
    TGCCAGGTGT GTATGAAAAG CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35386
    Clone ID Related Accession (Same CDS sequence) XM_010800459.2 , XM_002699886.5 , XM_002699886.4
    Accession Version XM_002699886.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-6(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002699886.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
    GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT
    AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA
    ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC
    TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC
    GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA
    CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC
    TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT
    GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA
    CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA
    CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA
    TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT
    CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA
    GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG
    CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC
    CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC
    CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG
    GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT
    GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA
    GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA
    CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT
    TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA
    GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA
    AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA
    CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA
    CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA
    CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG
    CAACCAGGCC CCCGTGGAGT TCCAGGTATC ACCTTGCCTT GTATTATTCC TGGACCGTAT
    GGTCCATCAG GACTCCCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC
    CCTGGGGCTC CAGGCCAACC TGGATTGCGT GGAAATAAAG GGGACCCCGG AAGTCCAGGA
    TTGGTTCACC TGCCTGAATT GCCAGGGTTT CCTGGACCTC ATGGAGAGAA GGGCTTACCT
    GGGTTTCCTG GGCTCCCTGG AAAAGACGGC TTGCCTGGGA AAGTTGGCAG CCCCGGCTTA
    CCAGGTCCCA AGGGAGCCCC TGGTGACATC CTTGGTGCTG AAAATGGTGC TCCGGGGGAG
    CAAGGCCTAC AGGGACTGCC TGGGGACAGA GGATTTCCTG GAGACTCTGG CCTTCCAGGA
    CTCAAGGGTT TGCTTGGGAA GTCGGGCTTG CTCGGCCCCA AAGGTGAGCG GGGCAGCCCT
    GGAATACCAG GCCGTGTGGG TCAGCCAGGA CCCCCAGGCC CTAGTGGTCT ATTTGGCATC
    AAGGGCAAAC CCGGGCTCCG CGGACCCCCA GGCTTTCCAG GCATTTCAGG ATATCCTGGA
    AAGAAAGGTC CAAGAGGCGA GATAGGTCTC CCTGCGTCAG CTGGAAAGAG AGGCCTGCCT
    GGGCTGAAAG GGCTTCCGGG ACTGCCAGGG CCAATTGGCT TCCTGGGGAA CTCAGGCTTG
    CCAGGGGCCA CTGGGTTACC AGGCCTGCCA GGTCTGAAGG GTGAGAAGGG GTCTGTTGGA
    CTGACAGGTT TTCCAGGGAT TCCAGGTCTC CCTGGTATTC CTGGAGCAAG TGGATTAAAG
    GGAATTCCTG GGTCGACAGG AAGAGTGGGG CCATCTGGAT GGGCTGGTAG CCCTGGTGAA
    AAAGGAGACA GAGGTGACCC AGGCCCCTTT GGGATACCCA GTCATCGACC TCCAATGCTG
    AACCTCCGCT TCAAAGGAGA CAAAGGTTCT CAGGGCTCAC CTGGAGCCAT TGGATTTCCT
    GGACCCAGAG GTGACAAAGG AGAGGCTGGC CACCCCGGGC CACCAGGCTT GCCTGGAGCT
    CCTGGCTTCC CCAGCCTCAT CAAGGGACTT AGTGGGAGAC CAGGCCCCCC TGGCTCCACA
    GGACTACGGG GCTTACCTGG CCTGAAGGGG TACCCTGGAA TTGCAGGTTT CCAAGGAATT
    CCAGGAGAAA GTGGTTCACA GGGTCTCAGT GGAGTTCCCG GGCTACCAGG AGCATCTGGT
    CTCCCAGGTT TAAAAGGAGA TCAAGGCCAG ACACTTGGAA TTTCTGGTAG CCCAGGACCC
    AAGGGACAAC CTGGGGAGTC TGGTTTCAAA GGTGTGAAAG GAAAAGACGG ACCAGTTGGT
    GATGTGGGTT TCCCAGGAAA CAAAGGTGAA GATGGGAAAG TTGGTATTTC TGGAGATGTT
    GGCCTTCCTG GCTCCCTAGG ACTCCCCGGA GTTGCAGGCA TGAGAGGAAA TCCAGGGCTT
    CCAGGTTCTC CAGGCCACCC AGGGGCAACT GGGCCCCTGG GTCCCCCTGG CCTAATAGGA
    ACCAAAGGTT TCCCTGGACT TCCAGGTTTA CATGGACTGA ATGGGCTTCC AGGAACCAAG
    GGGACTCATG GAACTCCAGG ACCTAGTATA ACCGGTGTGC CTGGGCCAGC TGGCTTGCCT
    GGTCCCAAAG GGGAAAAAGG TTCTCCAGGA ATTGGCATCG GAGCCCCAGG AAAGCCAGGT
    ATAAGAGGAC CCAAAGGTAG TCAAGGTTTT CCAGGTCTCC AGGGCGCCGC TGGTCTCCCC
    GGTGCACTGG GCCTCTCCTT GCCTTCAGTC ATAGAAGGAC AGCCTGGTGA CCCTGGGCGA
    CCAGGTCTAG ATGGAGAACG AGGCCGCCCA GGCCCTCCTG GGCCCCCAGG CCCCCCTGGG
    CCTGCCTCAG ATCAAGGCGA CCCTGGAGAC CCTGGCTTCC CTGGAATTCC TGGCCCTCAA
    GGGCCCAAAG GAGACCAAGG AATTCCAGGT TTCTCTGGCC TCCCTGGAGA GCTAGGATTG
    AAAGGCATGA GAGGTGAGCC TGGCTTCATG GGGACTCCAG GCAAGGTTGG ACCACCTGGA
    GACCCAGGAC TTCCCGGGAT GAAGGGGAAG GCGGGGCCAA GAGGCTTATC TGGCCCTCGA
    GGTGCTCCTG GACACACACC CATTGCAGAA GCCATCCAGG CTCCCCCTGG ACCCATGGGT
    CTTCCAGGCA TTGATGGAAT CCCTGGCCTC ATGGGAGACC CTGGGGTCCA AGGACCTGTG
    GGCCTACAAG GATCTAAAGG TTTACCTGGC ATCCCTGGCA AAGATGGCGC CAGTGGACTC
    CCTGGCCCAC CTGGGGCTCT TGGTGATCCT GGTCTCCCTG GACTACAAGG CCCTCCAGGA
    TTTGAAGGAG CTCCAGGGAA GCCGGGCCCC TTCGGGAGGG CTGGAGTGCC TGGACAGAGC
    CTGAGAGTGG GCTACACCTT GGTGAAGCAC AGCCAGTCAG AACAGGTGCC CCTGTGCCCC
    AGCGGGATGA GCCAGCTCTG GGTGGGTTAC AGCCTGCTGT TCGTGGAGGG GCAAGAGAAA
    GCCCACAACC AGGACCTGGG GTTTGCTGGC TCCTGCCTGC CCCGCTTCAG CACCATGCCA
    TTCATCTACT GCAACATCAA TGAAGTGTGC CACTATGCCC GACGGAATGA TAAGTCCTAC
    TGGCTCTCCA CCACCGCCCC CATCCCTATG ATGCCGGTCG GCCAGACACA GATCCCCCAG
    TACATCAGCC GCTGCTCTGT GTGTGAGGCG CCCTCGCAAG CCATTGCTGT GCACAGCCAG
    GACATCACCA TCCCACAGTG CCCCCTGGGC TGGCGCAGTC TGTGGATTGG GTACTCCTTC
    CTCATGCACA CAGCCGCCGG TGCAGAGGGC GGGGGCCAGT CCCTGGTCTC TCCCGGATCC
    TGCCTAGAGG ATTTCCGGGC TACTCCTTTC ATCGAATGCA GTGGTGCCCG GGGCACCTGC
    CACTACTTTG CCAACAAATA TAGTTTCTGG CTGACAACAG TGGAAGAGAG GCAGCAGTTT
    GGGGAGGAGC CTGTATCTGA GACGCTGAAA GCTGGACAGC TCCACACCCG AGTCAGCCGC
    TGCCAGGTGT GTATGAAAAG CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002699932.2
    CDS256..5322
    Translation

    Target ORF information:

    RefSeq Version XM_002699886.5
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 6 chain (COL4A6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002699886.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
    GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT
    AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA
    ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC
    TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC
    GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA
    CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC
    TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT
    GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA
    CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA
    CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA
    TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT
    CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA
    GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG
    CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC
    CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC
    CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG
    GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT
    GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA
    GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA
    CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT
    TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA
    GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA
    AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA
    CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA
    CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA
    CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG
    CAACCAGGCC CCCGTGGAGT TCCAGGTATC ACCTTGCCTT GTATTATTCC TGGACCGTAT
    GGTCCATCAG GACTCCCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC
    CCTGGGGCTC CAGGCCAACC TGGATTGCGT GGAAATAAAG GGGACCCCGG AAGTCCAGGA
    TTGGTTCACC TGCCTGAATT GCCAGGGTTT CCTGGACCTC ATGGAGAGAA GGGCTTACCT
    GGGTTTCCTG GGCTCCCTGG AAAAGACGGC TTGCCTGGGA AAGTTGGCAG CCCCGGCTTA
    CCAGGTCCCA AGGGAGCCCC TGGTGACATC CTTGGTGCTG AAAATGGTGC TCCGGGGGAG
    CAAGGCCTAC AGGGACTGCC TGGGGACAGA GGATTTCCTG GAGACTCTGG CCTTCCAGGA
    CTCAAGGGTT TGCTTGGGAA GTCGGGCTTG CTCGGCCCCA AAGGTGAGCG GGGCAGCCCT
    GGAATACCAG GCCGTGTGGG TCAGCCAGGA CCCCCAGGCC CTAGTGGTCT ATTTGGCATC
    AAGGGCAAAC CCGGGCTCCG CGGACCCCCA GGCTTTCCAG GCATTTCAGG ATATCCTGGA
    AAGAAAGGTC CAAGAGGCGA GATAGGTCTC CCTGCGTCAG CTGGAAAGAG AGGCCTGCCT
    GGGCTGAAAG GGCTTCCGGG ACTGCCAGGG CCAATTGGCT TCCTGGGGAA CTCAGGCTTG
    CCAGGGGCCA CTGGGTTACC AGGCCTGCCA GGTCTGAAGG GTGAGAAGGG GTCTGTTGGA
    CTGACAGGTT TTCCAGGGAT TCCAGGTCTC CCTGGTATTC CTGGAGCAAG TGGATTAAAG
    GGAATTCCTG GGTCGACAGG AAGAGTGGGG CCATCTGGAT GGGCTGGTAG CCCTGGTGAA
    AAAGGAGACA GAGGTGACCC AGGCCCCTTT GGGATACCCA GTCATCGACC TCCAATGCTG
    AACCTCCGCT TCAAAGGAGA CAAAGGTTCT CAGGGCTCAC CTGGAGCCAT TGGATTTCCT
    GGACCCAGAG GTGACAAAGG AGAGGCTGGC CACCCCGGGC CACCAGGCTT GCCTGGAGCT
    CCTGGCTTCC CCAGCCTCAT CAAGGGACTT AGTGGGAGAC CAGGCCCCCC TGGCTCCACA
    GGACTACGGG GCTTACCTGG CCTGAAGGGG TACCCTGGAA TTGCAGGTTT CCAAGGAATT
    CCAGGAGAAA GTGGTTCACA GGGTCTCAGT GGAGTTCCCG GGCTACCAGG AGCATCTGGT
    CTCCCAGGTT TAAAAGGAGA TCAAGGCCAG ACACTTGGAA TTTCTGGTAG CCCAGGACCC
    AAGGGACAAC CTGGGGAGTC TGGTTTCAAA GGTGTGAAAG GAAAAGACGG ACCAGTTGGT
    GATGTGGGTT TCCCAGGAAA CAAAGGTGAA GATGGGAAAG TTGGTATTTC TGGAGATGTT
    GGCCTTCCTG GCTCCCTAGG ACTCCCCGGA GTTGCAGGCA TGAGAGGAAA TCCAGGGCTT
    CCAGGTTCTC CAGGCCACCC AGGGGCAACT GGGCCCCTGG GTCCCCCTGG CCTAATAGGA
    ACCAAAGGTT TCCCTGGACT TCCAGGTTTA CATGGACTGA ATGGGCTTCC AGGAACCAAG
    GGGACTCATG GAACTCCAGG ACCTAGTATA ACCGGTGTGC CTGGGCCAGC TGGCTTGCCT
    GGTCCCAAAG GGGAAAAAGG TTCTCCAGGA ATTGGCATCG GAGCCCCAGG AAAGCCAGGT
    ATAAGAGGAC CCAAAGGTAG TCAAGGTTTT CCAGGTCTCC AGGGCGCCGC TGGTCTCCCC
    GGTGCACTGG GCCTCTCCTT GCCTTCAGTC ATAGAAGGAC AGCCTGGTGA CCCTGGGCGA
    CCAGGTCTAG ATGGAGAACG AGGCCGCCCA GGCCCTCCTG GGCCCCCAGG CCCCCCTGGG
    CCTGCCTCAG ATCAAGGCGA CCCTGGAGAC CCTGGCTTCC CTGGAATTCC TGGCCCTCAA
    GGGCCCAAAG GAGACCAAGG AATTCCAGGT TTCTCTGGCC TCCCTGGAGA GCTAGGATTG
    AAAGGCATGA GAGGTGAGCC TGGCTTCATG GGGACTCCAG GCAAGGTTGG ACCACCTGGA
    GACCCAGGAC TTCCCGGGAT GAAGGGGAAG GCGGGGCCAA GAGGCTTATC TGGCCCTCGA
    GGTGCTCCTG GACACACACC CATTGCAGAA GCCATCCAGG CTCCCCCTGG ACCCATGGGT
    CTTCCAGGCA TTGATGGAAT CCCTGGCCTC ATGGGAGACC CTGGGGTCCA AGGACCTGTG
    GGCCTACAAG GATCTAAAGG TTTACCTGGC ATCCCTGGCA AAGATGGCGC CAGTGGACTC
    CCTGGCCCAC CTGGGGCTCT TGGTGATCCT GGTCTCCCTG GACTACAAGG CCCTCCAGGA
    TTTGAAGGAG CTCCAGGGAA GCCGGGCCCC TTCGGGAGGG CTGGAGTGCC TGGACAGAGC
    CTGAGAGTGG GCTACACCTT GGTGAAGCAC AGCCAGTCAG AACAGGTGCC CCTGTGCCCC
    AGCGGGATGA GCCAGCTCTG GGTGGGTTAC AGCCTGCTGT TCGTGGAGGG GCAAGAGAAA
    GCCCACAACC AGGACCTGGG GTTTGCTGGC TCCTGCCTGC CCCGCTTCAG CACCATGCCA
    TTCATCTACT GCAACATCAA TGAAGTGTGC CACTATGCCC GACGGAATGA TAAGTCCTAC
    TGGCTCTCCA CCACCGCCCC CATCCCTATG ATGCCGGTCG GCCAGACACA GATCCCCCAG
    TACATCAGCC GCTGCTCTGT GTGTGAGGCG CCCTCGCAAG CCATTGCTGT GCACAGCCAG
    GACATCACCA TCCCACAGTG CCCCCTGGGC TGGCGCAGTC TGTGGATTGG GTACTCCTTC
    CTCATGCACA CAGCCGCCGG TGCAGAGGGC GGGGGCCAGT CCCTGGTCTC TCCCGGATCC
    TGCCTAGAGG ATTTCCGGGC TACTCCTTTC ATCGAATGCA GTGGTGCCCG GGGCACCTGC
    CACTACTTTG CCAACAAATA TAGTTTCTGG CTGACAACAG TGGAAGAGAG GCAGCAGTTT
    GGGGAGGAGC CTGTATCTGA GACGCTGAAA GCTGGACAGC TCCACACCCG AGTCAGCCGC
    TGCCAGGTGT GTATGAAAAG CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35386
    Clone ID Related Accession (Same CDS sequence) XM_010800459.2 , XM_002699886.5 , XM_002699886.4
    Accession Version XM_002699886.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-6(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104715.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002699886.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
    GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT
    AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA
    ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC
    TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC
    GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA
    CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC
    TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT
    GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA
    CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA
    CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA
    TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT
    CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA
    GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG
    CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC
    CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC
    CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG
    GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT
    GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA
    GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA
    CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT
    TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA
    GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA
    AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA
    CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA
    CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA
    CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG
    CAACCAGGCC CCCGTGGAGT TCCAGGTATC ACCTTGCCTT GTATTATTCC TGGACCGTAT
    GGTCCATCAG GACTCCCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC
    CCTGGGGCTC CAGGCCAACC TGGATTGCGT GGAAATAAAG GGGACCCCGG AAGTCCAGGA
    TTGGTTCACC TGCCTGAATT GCCAGGGTTT CCTGGACCTC ATGGAGAGAA GGGCTTACCT
    GGGTTTCCTG GGCTCCCTGG AAAAGACGGC TTGCCTGGGA AAGTTGGCAG CCCCGGCTTA
    CCAGGTCCCA AGGGAGCCCC TGGTGACATC CTTGGTGCTG AAAATGGTGC TCCGGGGGAG
    CAAGGCCTAC AGGGACTGCC TGGGGACAGA GGATTTCCTG GAGACTCTGG CCTTCCAGGA
    CTCAAGGGTT TGCTTGGGAA GTCGGGCTTG CTCGGCCCCA AAGGTGAGCG GGGCAGCCCT
    GGAATACCAG GCCGTGTGGG TCAGCCAGGA CCCCCAGGCC CTAGTGGTCT ATTTGGCATC
    AAGGGCAAAC CCGGGCTCCG CGGACCCCCA GGCTTTCCAG GCATTTCAGG ATATCCTGGA
    AAGAAAGGTC CAAGAGGCGA GATAGGTCTC CCTGCGTCAG CTGGAAAGAG AGGCCTGCCT
    GGGCTGAAAG GGCTTCCGGG ACTGCCAGGG CCAATTGGCT TCCTGGGGAA CTCAGGCTTG
    CCAGGGGCCA CTGGGTTACC AGGCCTGCCA GGTCTGAAGG GTGAGAAGGG GTCTGTTGGA
    CTGACAGGTT TTCCAGGGAT TCCAGGTCTC CCTGGTATTC CTGGAGCAAG TGGATTAAAG
    GGAATTCCTG GGTCGACAGG AAGAGTGGGG CCATCTGGAT GGGCTGGTAG CCCTGGTGAA
    AAAGGAGACA GAGGTGACCC AGGCCCCTTT GGGATACCCA GTCATCGACC TCCAATGCTG
    AACCTCCGCT TCAAAGGAGA CAAAGGTTCT CAGGGCTCAC CTGGAGCCAT TGGATTTCCT
    GGACCCAGAG GTGACAAAGG AGAGGCTGGC CACCCCGGGC CACCAGGCTT GCCTGGAGCT
    CCTGGCTTCC CCAGCCTCAT CAAGGGACTT AGTGGGAGAC CAGGCCCCCC TGGCTCCACA
    GGACTACGGG GCTTACCTGG CCTGAAGGGG TACCCTGGAA TTGCAGGTTT CCAAGGAATT
    CCAGGAGAAA GTGGTTCACA GGGTCTCAGT GGAGTTCCCG GGCTACCAGG AGCATCTGGT
    CTCCCAGGTT TAAAAGGAGA TCAAGGCCAG ACACTTGGAA TTTCTGGTAG CCCAGGACCC
    AAGGGACAAC CTGGGGAGTC TGGTTTCAAA GGTGTGAAAG GAAAAGACGG ACCAGTTGGT
    GATGTGGGTT TCCCAGGAAA CAAAGGTGAA GATGGGAAAG TTGGTATTTC TGGAGATGTT
    GGCCTTCCTG GCTCCCTAGG ACTCCCCGGA GTTGCAGGCA TGAGAGGAAA TCCAGGGCTT
    CCAGGTTCTC CAGGCCACCC AGGGGCAACT GGGCCCCTGG GTCCCCCTGG CCTAATAGGA
    ACCAAAGGTT TCCCTGGACT TCCAGGTTTA CATGGACTGA ATGGGCTTCC AGGAACCAAG
    GGGACTCATG GAACTCCAGG ACCTAGTATA ACCGGTGTGC CTGGGCCAGC TGGCTTGCCT
    GGTCCCAAAG GGGAAAAAGG TTCTCCAGGA ATTGGCATCG GAGCCCCAGG AAAGCCAGGT
    ATAAGAGGAC CCAAAGGTAG TCAAGGTTTT CCAGGTCTCC AGGGCGCCGC TGGTCTCCCC
    GGTGCACTGG GCCTCTCCTT GCCTTCAGTC ATAGAAGGAC AGCCTGGTGA CCCTGGGCGA
    CCAGGTCTAG ATGGAGAACG AGGCCGCCCA GGCCCTCCTG GGCCCCCAGG CCCCCCTGGG
    CCTGCCTCAG ATCAAGGCGA CCCTGGAGAC CCTGGCTTCC CTGGAATTCC TGGCCCTCAA
    GGGCCCAAAG GAGACCAAGG AATTCCAGGT TTCTCTGGCC TCCCTGGAGA GCTAGGATTG
    AAAGGCATGA GAGGTGAGCC TGGCTTCATG GGGACTCCAG GCAAGGTTGG ACCACCTGGA
    GACCCAGGAC TTCCCGGGAT GAAGGGGAAG GCGGGGCCAA GAGGCTTATC TGGCCCTCGA
    GGTGCTCCTG GACACACACC CATTGCAGAA GCCATCCAGG CTCCCCCTGG ACCCATGGGT
    CTTCCAGGCA TTGATGGAAT CCCTGGCCTC ATGGGAGACC CTGGGGTCCA AGGACCTGTG
    GGCCTACAAG GATCTAAAGG TTTACCTGGC ATCCCTGGCA AAGATGGCGC CAGTGGACTC
    CCTGGCCCAC CTGGGGCTCT TGGTGATCCT GGTCTCCCTG GACTACAAGG CCCTCCAGGA
    TTTGAAGGAG CTCCAGGGAA GCCGGGCCCC TTCGGGAGGG CTGGAGTGCC TGGACAGAGC
    CTGAGAGTGG GCTACACCTT GGTGAAGCAC AGCCAGTCAG AACAGGTGCC CCTGTGCCCC
    AGCGGGATGA GCCAGCTCTG GGTGGGTTAC AGCCTGCTGT TCGTGGAGGG GCAAGAGAAA
    GCCCACAACC AGGACCTGGG GTTTGCTGGC TCCTGCCTGC CCCGCTTCAG CACCATGCCA
    TTCATCTACT GCAACATCAA TGAAGTGTGC CACTATGCCC GACGGAATGA TAAGTCCTAC
    TGGCTCTCCA CCACCGCCCC CATCCCTATG ATGCCGGTCG GCCAGACACA GATCCCCCAG
    TACATCAGCC GCTGCTCTGT GTGTGAGGCG CCCTCGCAAG CCATTGCTGT GCACAGCCAG
    GACATCACCA TCCCACAGTG CCCCCTGGGC TGGCGCAGTC TGTGGATTGG GTACTCCTTC
    CTCATGCACA CAGCCGCCGG TGCAGAGGGC GGGGGCCAGT CCCTGGTCTC TCCCGGATCC
    TGCCTAGAGG ATTTCCGGGC TACTCCTTTC ATCGAATGCA GTGGTGCCCG GGGCACCTGC
    CACTACTTTG CCAACAAATA TAGTTTCTGG CTGACAACAG TGGAAGAGAG GCAGCAGTTT
    GGGGAGGAGC CTGTATCTGA GACGCTGAAA GCTGGACAGC TCCACACCCG AGTCAGCCGC
    TGCCAGGTGT GTATGAAAAG CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002699932.2
    CDS270..5336
    Translation

    Target ORF information:

    RefSeq Version XM_002699886.4
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 6 (COL4A6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002699886.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACCCTG GGTTGTGGCT GCTTCTGGTT ACTTTGTGCC TGACCGAGGA ACTGGCAGGA 
    GCGGGAGAGA AGAAGCCCTA TGGGAAGCCA TGTGGGGGCC AAGACTGCAG TGGGGGCTGT
    AAGTGCTCTC CTGAGAAAGG AGCCAGAGGG AGACCAGGAC CAATTGGAAT TCAAGGTCCA
    ACAGGTCCTC AAGGACTTCC TGGCCCTAGT GGTTTATCAG GGTTAAAAGG AGAAAGGGGC
    TCCCCAGGCC CGCTGGGACC GTATGGACCA AAAGGCGATA AGGGTCCCAT GGGTGTTCCC
    GGCTTTCTTG GCATCAATGG AATTCCAGGC CACCCTGGAC AGCCAGGACC CAGAGGCCCA
    CCTGGCCTGG ATGGCTGTAA TGGAACTCAA GGAGCTGTTG GATTTCCAGG CCCTGATGGC
    TATTCTGGGC TTCCTGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTAGTTT CAAAGGAATG AAGGGGGAGT CTGGGCTGCC CGGACTGGAT
    GGAATCACTG GCCCACAAGG AGCACCCGGA TCTCCAGGAG CTGTAGGACC CAGAGGACCA
    CCAGGATTGC AAGGTCCTCC TGGCCCCCCT GGTTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGAGATGTTG GCCTGCCTGG TCCTGCAGGA
    CCCCCACCAT CTACTGGAGA ACTGGAATTC ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCCTCA GGGCTTCCCA GGAGTAAGTG GTCCACCTGG TCTGCCGGGA
    TTTGGAATTG CTGGAGAAAA AGGAGAAAAG GGAATTCCTG GTTTGCCAGG ACCTAGGGGT
    CCCATGGGTT TAGAAGGAGT CCAAGGCCCT CCGGGGAGTC AGGGCAGGAA GGGGTCTTCA
    GGTTTCCCCG GGCTTAATGG ATTCCCAGGA ATTAAGGGTG AAAAAGGTGG CATTGGCCTG
    CCAGGCCCAG ATATTTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAC
    CCTGGTATGC CAGGCCTCCC AGGCCTTAAA GGAGATGAAG GCATACAGGG CCTACCTGGC
    CCTCCTGGCG CCCCTGGATT ACCAGCTTTA CCAGGTGGCC CCGGTGCTCT GGGGCCCCAG
    GGAGCTCTAG GCCTGAAGGG GGAACGAGGA AACACTGGCC GGACCACAAC GGGGGCAGCT
    GGTCTCCCTG GCAGAGATGG TTTGCCAGGC CTGCCAGGCC TACCAGGCCC ACCTGGTGCA
    GCATCTGAGA CGGGAACCCT ACAGAGCACA GAACCGGGAT CGCCGGGACT CCGAGGGGAA
    CGAGGTCCAA AAGGACACCC AGGCCTCAAA GGAATTAAAG GGGACCCAGG TTTTTGTGCT
    TGTGATGGTG GTGTCCCCAA CATGGGGCCA CCTGGGGAGC CAGGCCTGCC TGGGCCACCA
    GGACTTCTGG GCCTTCCAGG TCTTAAAGGA ACCAAAGGTG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGATCC AGGTGTATTT GGGCCTCGAG GTCCAACAGG CCCTAAAGGA
    AAGAAAGGGG ACCCAACTCT CAGCACAAGA TCAGGGATGC CAGGAGAGCA AGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG TGAGACAGGA GCCCCAGGCA AGGATGGAAT ACCAGGTTTA
    CCAGGGCTGC CAGGCCTTCT GGGTGATGGT GCACAGGGCT TCCCCGGTGA AAAGGGGTTA
    CCAGGACTTC CTGGTGAAAA AGGCCACACT GGTCCAACTG GCCCCCCAGG AATTGGGCTA
    CCAGGGGCTC CTGGACCTCG TGGACTTCCT GGAGATAAAG GAGTAGATGG ATTACCAGGG
    CAACCAGGCC CCCGTGGAGT TCCAGGTATC ACCTTGCCTT GTATTATTCC TGGACCGTAT
    GGTCCATCAG GACTCCCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC
    CCTGGGGCTC CAGGCCAACC TGGATTGCGT GGAAATAAAG GGGACCCCGG AAGTCCAGGA
    TTGGTTCACC TGCCTGAATT GCCAGGGTTT CCTGGACCTC ATGGAGAGAA GGGCTTACCT
    GGGTTTCCTG GGCTCCCTGG AAAAGACGGC TTGCCTGGGA AAGTTGGCAG CCCCGGCTTA
    CCAGGTCCCA AGGGAGCCCC TGGTGACATC CTTGGTGCTG AAAATGGTGC TCCGGGGGAG
    CAAGGCCTAC AGGGACTGCC TGGGGACAGA GGATTTCCTG GAGACTCTGG CCTTCCAGGA
    CTCAAGGGTT TGCTTGGGAA GTCGGGCTTG CTCGGCCCCA AAGGTGAGCG GGGCAGCCCT
    GGAATACCAG GCCGTGTGGG TCAGCCAGGA CCCCCAGGCC CTAGTGGTCT ATTTGGCATC
    AAGGGCAAAC CCGGGCTCCG CGGACCCCCA GGCTTTCCAG GCATTTCAGG ATATCCTGGA
    AAGAAAGGTC CAAGAGGCGA GATAGGTCTC CCTGCGTCAG CTGGAAAGAG AGGCCTGCCT
    GGGCTGAAAG GGCTTCCGGG ACTGCCAGGG CCAATTGGCT TCCTGGGGAA CTCAGGCTTG
    CCAGGGGCCA CTGGGTTACC AGGCCTGCCA GGTCTGAAGG GTGAGAAGGG GTCTGTTGGA
    CTGACAGGTT TTCCAGGGAT TCCAGGTCTC CCTGGTATTC CTGGAGCAAG TGGATTAAAG
    GGAATTCCTG GGTCGACAGG AAGAGTGGGG CCATCTGGAT GGGCTGGTAG CCCTGGTGAA
    AAAGGAGACA GAGGTGACCC AGGCCCCTTT GGGATACCCA GTCATCGACC TCCAATGCTG
    AACCTCCGCT TCAAAGGAGA CAAAGGTTCT CAGGGCTCAC CTGGAGCCAT TGGATTTCCT
    GGACCCAGAG GTGACAAAGG AGAGGCTGGC CACCCCGGGC CACCAGGCTT GCCTGGAGCT
    CCTGGCTTCC CCAGCCTCAT CAAGGGACTT AGTGGGAGAC CAGGCCCCCC TGGCTCCACA
    GGACTACGGG GCTTACCTGG CCTGAAGGGG TACCCTGGAA TTGCAGGTTT CCAAGGAATT
    CCAGGAGAAA GTGGTTCACA GGGTCTCAGT GGAGTTCCCG GGCTACCAGG AGCATCTGGT
    CTCCCAGGTT TAAAAGGAGA TCAAGGCCAG ACACTTGGAA TTTCTGGTAG CCCAGGACCC
    AAGGGACAAC CTGGGGAGTC TGGTTTCAAA GGTGTGAAAG GAAAAGACGG ACCAGTTGGT
    GATGTGGGTT TCCCAGGAAA CAAAGGTGAA GATGGGAAAG TTGGTATTTC TGGAGATGTT
    GGCCTTCCTG GCTCCCTAGG ACTCCCCGGA GTTGCAGGCA TGAGAGGAAA TCCAGGGCTT
    CCAGGTTCTC CAGGCCACCC AGGGGCAACT GGGCCCCTGG GTCCCCCTGG CCTAATAGGA
    ACCAAAGGTT TCCCTGGACT TCCAGGTTTA CATGGACTGA ATGGGCTTCC AGGAACCAAG
    GGGACTCATG GAACTCCAGG ACCTAGTATA ACCGGTGTGC CTGGGCCAGC TGGCTTGCCT
    GGTCCCAAAG GGGAAAAAGG TTCTCCAGGA ATTGGCATCG GAGCCCCAGG AAAGCCAGGT
    ATAAGAGGAC CCAAAGGTAG TCAAGGTTTT CCAGGTCTCC AGGGCGCCGC TGGTCTCCCC
    GGTGCACTGG GCCTCTCCTT GCCTTCAGTC ATAGAAGGAC AGCCTGGTGA CCCTGGGCGA
    CCAGGTCTAG ATGGAGAACG AGGCCGCCCA GGCCCTCCTG GGCCCCCAGG CCCCCCTGGG
    CCTGCCTCAG ATCAAGGCGA CCCTGGAGAC CCTGGCTTCC CTGGAATTCC TGGCCCTCAA
    GGGCCCAAAG GAGACCAAGG AATTCCAGGT TTCTCTGGCC TCCCTGGAGA GCTAGGATTG
    AAAGGCATGA GAGGTGAGCC TGGCTTCATG GGGACTCCAG GCAAGGTTGG ACCACCTGGA
    GACCCAGGAC TTCCCGGGAT GAAGGGGAAG GCGGGGCCAA GAGGCTTATC TGGCCCTCGA
    GGTGCTCCTG GACACACACC CATTGCAGAA GCCATCCAGG CTCCCCCTGG ACCCATGGGT
    CTTCCAGGCA TTGATGGAAT CCCTGGCCTC ATGGGAGACC CTGGGGTCCA AGGACCTGTG
    GGCCTACAAG GATCTAAAGG TTTACCTGGC ATCCCTGGCA AAGATGGCGC CAGTGGACTC
    CCTGGCCCAC CTGGGGCTCT TGGTGATCCT GGTCTCCCTG GACTACAAGG CCCTCCAGGA
    TTTGAAGGAG CTCCAGGGAA GCCGGGCCCC TTCGGGAGGG CTGGAGTGCC TGGACAGAGC
    CTGAGAGTGG GCTACACCTT GGTGAAGCAC AGCCAGTCAG AACAGGTGCC CCTGTGCCCC
    AGCGGGATGA GCCAGCTCTG GGTGGGTTAC AGCCTGCTGT TCGTGGAGGG GCAAGAGAAA
    GCCCACAACC AGGACCTGGG GTTTGCTGGC TCCTGCCTGC CCCGCTTCAG CACCATGCCA
    TTCATCTACT GCAACATCAA TGAAGTGTGC CACTATGCCC GACGGAATGA TAAGTCCTAC
    TGGCTCTCCA CCACCGCCCC CATCCCTATG ATGCCGGTCG GCCAGACACA GATCCCCCAG
    TACATCAGCC GCTGCTCTGT GTGTGAGGCG CCCTCGCAAG CCATTGCTGT GCACAGCCAG
    GACATCACCA TCCCACAGTG CCCCCTGGGC TGGCGCAGTC TGTGGATTGG GTACTCCTTC
    CTCATGCACA CAGCCGCCGG TGCAGAGGGC GGGGGCCAGT CCCTGGTCTC TCCCGGATCC
    TGCCTAGAGG ATTTCCGGGC TACTCCTTTC ATCGAATGCA GTGGTGCCCG GGGCACCTGC
    CACTACTTTG CCAACAAATA TAGTTTCTGG CTGACAACAG TGGAAGAGAG GCAGCAGTTT
    GGGGAGGAGC CTGTATCTGA GACGCTGAAA GCTGGACAGC TCCACACCCG AGTCAGCCGC
    TGCCAGGTGT GTATGAAAAG CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.