CLP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLP1
Entrez Gene ID	10978
Full Name	cleavage and polyadenylation factor I subunit 1
Synonyms	HEAB,hClp1
General protein information	Preferred Names cleavage and polyadenylation factor I subunit 1 Names polyribonucleotide 5'-hydroxyl-kinase Clp1 ATP/GTP-binding protein homolog of yeast CFIA subunit Clp1p polyadenylation factor Clp1 polynucleotide kinase Clp1 pre-mRNA cleavage complex II protein Clp1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q12.1
Summary	This gene encodes a member of the Clp1 family. The encoded protein is a multifunctional kinase which is a component of the tRNA splicing endonuclease complex and a component of the pre-mRNA cleavage complex II. This protein is implicated in tRNA, mRNA, and siRNA maturation. Mutations in this gene are associated with pontocerebellar hypoplasia type 10 (PCH10). Alternatively splice transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014].
Disorder MIM:	608757
Disorder Html:	Pontocerebellar hypoplasia, type 10, 615803 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_006831.3	NP_006822.1	polyribonucleotide 5'-hydroxyl-kinase Clp1 isoform 1
NM_006831.2	NP_006822.1	polyribonucleotide 5'-hydroxyl-kinase Clp1 isoform 1
NM_001142597.1	NP_001136069.1	polyribonucleotide 5'-hydroxyl-kinase Clp1 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	CLP1	NP_006822.1
	CLP1	XP_001101762.1
Drosophila melanogaster (fruit fly)	cbc	NP_610876.1
Arabidopsis thaliana (thale cress)	CLPS3	NP_974220.1
Oryza sativa (rice)	Os02g0217500	NP_001046299.1
Gallus gallus (chicken)	CLP1	NP_001012292.1
Mus musculus (house mouse)	Clp1	NP_598601.1
Rattus norvegicus (Norway rat)	Clp1	NP_001009599.1
Danio rerio (zebrafish)	LOC565621	XP_693984.3
Anopheles gambiae (African malaria mosquito)	CLP1_ANOGA	XP_308174.2
Caenorhabditis elegans (roundworm)	clpf-1	NP_001040858.1
Xenopus tropicalis (tropical clawed frog)	clp1	NP_001008002.1
Canis lupus familiaris (dog)	CLP1	XP_005631257.1
Bos taurus (cattle)	CLP1	NP_001075182.1

Gene Ontology
Bibliography

Related articles in PubMed

Biological insights from 108 schizophrenia-associated genetic loci.

Nature511(7510)421-7(2014 Jul)

Human CLP1 mutations alter tRNA biogenesis, affecting both peripheral and central nervous system function.
Karaca E, Weitzer S, Pehlivan D, Shiraishi H, Gogakos T, Hanada T, Jhangiani SN, Wiszniewski W, Withers M, Campbell IM, Erdin S, Isikay S, Franco LM, Gonzaga-Jauregui C, Gambin T, Gelowani V, Hunter JV, Yesil G, Koparir E, Yilmaz S, Brown M, Briskin D, Hafner M, Morozov P, Farazi TA, Bernreuther C, Glatzel M, Trattnig S, Friske J, Kronnerwetter C, Bainbridge MN, Gezdirici A, Seven M, Muzny DM, Boerwinkle E, Ozen M, , Clausen T, Tuschl T, Yuksel A, Hess A, Gibbs RA, Martinez J, Penninger JM, Lupski JR
Cell157(3)636-50(2014 Apr)

CLP1 founder mutation links tRNA splicing and maturation to cerebellar development and neurodegeneration.
Schaffer AE, Eggens VR, Caglayan AO, Reuter MS, Scott E, Coufal NG, Silhavy JL, Xue Y, Kayserili H, Yasuno K, Rosti RO, Abdellateef M, Caglar C, Kasher PR, Cazemier JL, Weterman MA, Cantagrel V, Cai N, Zweier C, Altunoglu U, Satkin NB, Aktar F, Tuysuz B, Yalcinkaya C, Caksen H, Bilguvar K, Fu XD, Trotta CR, Gabriel S, Reis A, Gunel M, Baas F, Gleeson JG
Cell157(3)651-63(2014 Apr)

Human RNA 5'-kinase (hClp1) can function as a tRNA splicing enzyme in vivo.
Ramirez A, Shuman S, Schwer B
RNA (New York, N.Y.)14(9)1737-45(2008 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study reports the identification of a human CLP1 mutation that impairs tRNA splicing in vitro and causes a neurological syndrome involving both the central nervous system (CNS) and peripheral nervous system (PNS).
Title: Human CLP1 mutations alter tRNA biogenesis, affecting both peripheral and central nervous system function.

Data link tRNA maturation to neuronal development and neurodegeneration through defective CLP1 function in humans.
Title: CLP1 founder mutation links tRNA splicing and maturation to cerebellar development and neurodegeneration.

Expression of hClp1 in budding yeast can complement conditional and lethal mutations in the essential 5'-OH RNA kinase module of yeast or plant tRNA ligases.
Title: Human RNA 5'-kinase (hClp1) can function as a tRNA splicing enzyme in vivo.

the kinase hClp1 phosphorylates and licenses synthetic siRNAs to become assembled into RISC for subsequent target RNA cleavage
Title: The human RNA kinase hClp1 is active on 3' transfer RNA exons and short interfering RNAs.

HEAB is a pre-mRNA 3'-end processing factor, which bridges two other pre-mRNA processing factors (CF Im and CPSF) and is involved in cleavage of the pre-mRNA. It can be UV-crosslinked to GTP.
Title: Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors.

The following CLP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26557	NM_006831.3 Latest version!	Homo sapiens cleavage factor polyribonucleotide kinase subunit 1 (CLP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu26511	NM_001142597.1 Latest version!	Homo sapiens cleavage factor polyribonucleotide kinase subunit 1 (CLP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$209.30 $299.00
View Old Accession Versions >>
OHu26557	NM_006831.2	Homo sapiens cleavage and polyadenylation factor I subunit 1 (CLP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLP1 ( NM_006831.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006822.1 Homo sapiens cleavage factor polyribonucleotide kinase subunit 1 (CLP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26557
Clone ID Related Accession (Same CDS sequence)	NM_006831.3 , NM_006831.2
Accession Version	NM_006831.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	polyribonucleotide 5'-hydroxyl-kinase Clp1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA922553.1 and BC000446.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_006831.2. Transcript Variant: This variant (1) represents the longer transcript and encodes a longer protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: U73524.1, SRR1803612.125930.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000533682.2/ ENSP00000434995.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGAGAAG AGGCTAATGA TGACAAGAAG CCAACCACTA AATTTGAACT AGAGCGAGAA 
ACAGAACTTC GCTTTGAGGT GGAGGCATCT CAGTCAGTTC AGTTGGAGTT GTTGACTGGC 
ATGGCAGAGA TCTTTGGCAC AGAGCTGACC CGAAACAAGA AATTCACCTT TGATGCTGGT 
GCCAAGGTGG CTGTTTTCAC TTGGCATGGC TGTTCTGTGC AACTGAGCGG CCGCACTGAG 
GTGGCTTATG TCTCCAAGGA CACTCCTATG TTGCTTTACC TCAACACTCA CACAGCCTTG 
GAACAGATGC GGAGGCAAGC GGAAAAGGAA GAAGAGCGAG GTCCCCGAGT GATGGTAGTG 
GGCCCCACTG ATGTGGGCAA GTCTACAGTG TGTCGCCTTC TGCTCAACTA CGCAGTGCGT 
TTGGGCCGCC GTCCCACTTA TGTGGAGCTG GATGTGGGCC AGGGTTCTGT GTCCATCCCT 
GGTACCATGG GGGCCCTCTA CATCGAGCGG CCTGCAGATG TCGAAGAGGG TTTCTCTATC 
CAGGCCCCTC TGGTGTATCA TTTTGGTTCC ACCACTCCTG GCACTAACAT CAAGCTTTAT 
AATAAGATTA CATCTCGTTT AGCAGATGTG TTCAACCAAA GGTGTGAGGT GAACCGAAGG 
GCATCTGTGA GTGGCTGTGT CATTAACACC TGTGGCTGGG TCAAGGGCTC TGGTTACCAG 
GCTCTGGTGC ATGCAGCCTC AGCTTTTGAG GTGGATGTCG TTGTTGTTCT GGATCAAGAA 
CGACTGTACA ATGAACTGAA ACGGGACCTC CCCCACTTTG TACGCACTGT GCTGCTCCCT 
AAATCTGGGG GTGTGGTGGA GCGCTCCAAG GACTTCCGGC GGGAATGTAG GGATGAGCGT 
ATCCGTGAGT ATTTTTATGG ATTCCGAGGC TGTTTCTATC CCCATGCCTT CAATGTCAAA 
TTTTCAGATG TGAAAATCTA CAAAGTTGGG GCACCCACCA TCCCAGACTC CTGTTTACCT 
TTGGGCATGT CTCAAGAGGA TAATCAGCTC AAGCTAGTAC CTGTCACTCC TGGGCGAGAT 
ATGGTGCACC ACCTACTGAG TGTTAGCACT GCCGAGGGTA CAGAGGAGAA CCTGTCCGAG 
ACAAGTGTAG CTGGCTTCAT TGTGGTGACC AGTGTGGACC TGGAGCATCA GGTGTTTACT 
GTTCTGTCTC CAGCCCCTCG CCCACTGCCT AAGAACTTCC TTCTCATCAT GGATATCCGG 
TTCATGGATC TGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006822.1
CDS	122..1399
Translation	MGEEANDDKKPTTKFELERETELRFEVEASQSVQLELLTGMAEIFGTELTRNKKFTFDAGAKVAVFTWHGCSVQLSGRTEVAYVSKDTPMLLYLNTHTALEQMRRQAEKEEERGPRVMVVGPTDVGKSTVCRLLLNYAVRLGRRPTYVELDVGQGSVSIPGTMGALYIERPADVEEGFSIQAPLVYHFGSTTPGTNIKLYNKITSRLADVFNQRCEVNRRASVSGCVINTCGWVKGSGYQALVHAASAFEVDVVVVLDQERLYNELKRDLPHFVRTVLLPKSGGVVERSKDFRRECRDERIREYFYGFRGCFYPHAFNVKFSDVKIYKVGAPTIPDSCLPLGMSQEDNQLKLVPVTPGRDMVHHLLSVSTAEGTEENLSETSVAGFIVVTSVDLEHQVFTVLSPAPRPLPKNFLLIMDIRFMDLK

Target ORF information:

RefSeq Version	NM_006831.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cleavage factor polyribonucleotide kinase subunit 1 (CLP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006831.3

ORF Insert Sequence:

ATGGGAGAAG AGGCTAATGA TGACAAGAAG CCAACCACTA AATTTGAACT AGAGCGAGAA 
ACAGAACTTC GCTTTGAGGT GGAGGCATCT CAGTCAGTTC AGTTGGAGTT GTTGACTGGC 
ATGGCAGAGA TCTTTGGCAC AGAGCTGACC CGAAACAAGA AATTCACCTT TGATGCTGGT 
GCCAAGGTGG CTGTTTTCAC TTGGCATGGC TGTTCTGTGC AACTGAGCGG CCGCACTGAG 
GTGGCTTATG TCTCCAAGGA CACTCCTATG TTGCTTTACC TCAACACTCA CACAGCCTTG 
GAACAGATGC GGAGGCAAGC GGAAAAGGAA GAAGAGCGAG GTCCCCGAGT GATGGTAGTG 
GGCCCCACTG ATGTGGGCAA GTCTACAGTG TGTCGCCTTC TGCTCAACTA CGCAGTGCGT 
TTGGGCCGCC GTCCCACTTA TGTGGAGCTG GATGTGGGCC AGGGTTCTGT GTCCATCCCT 
GGTACCATGG GGGCCCTCTA CATCGAGCGG CCTGCAGATG TCGAAGAGGG TTTCTCTATC 
CAGGCCCCTC TGGTGTATCA TTTTGGTTCC ACCACTCCTG GCACTAACAT CAAGCTTTAT 
AATAAGATTA CATCTCGTTT AGCAGATGTG TTCAACCAAA GGTGTGAGGT GAACCGAAGG 
GCATCTGTGA GTGGCTGTGT CATTAACACC TGTGGCTGGG TCAAGGGCTC TGGTTACCAG 
GCTCTGGTGC ATGCAGCCTC AGCTTTTGAG GTGGATGTCG TTGTTGTTCT GGATCAAGAA 
CGACTGTACA ATGAACTGAA ACGGGACCTC CCCCACTTTG TACGCACTGT GCTGCTCCCT 
AAATCTGGGG GTGTGGTGGA GCGCTCCAAG GACTTCCGGC GGGAATGTAG GGATGAGCGT 
ATCCGTGAGT ATTTTTATGG ATTCCGAGGC TGTTTCTATC CCCATGCCTT CAATGTCAAA 
TTTTCAGATG TGAAAATCTA CAAAGTTGGG GCACCCACCA TCCCAGACTC CTGTTTACCT 
TTGGGCATGT CTCAAGAGGA TAATCAGCTC AAGCTAGTAC CTGTCACTCC TGGGCGAGAT 
ATGGTGCACC ACCTACTGAG TGTTAGCACT GCCGAGGGTA CAGAGGAGAA CCTGTCCGAG 
ACAAGTGTAG CTGGCTTCAT TGTGGTGACC AGTGTGGACC TGGAGCATCA GGTGTTTACT 
GTTCTGTCTC CAGCCCCTCG CCCACTGCCT AAGAACTTCC TTCTCATCAT GGATATCCGG 
TTCATGGATC TGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLP1 ( NM_006831.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_006822.1 Homo sapiens cleavage and polyadenylation factor I subunit 1 (CLP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26557
Clone ID Related Accession (Same CDS sequence)	NM_006831.3 , NM_006831.2
Accession Version	NM_006831.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	polyribonucleotide 5'-hydroxyl-kinase Clp1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA922553.1 and BC000446.1. This sequence is a reference standard in the RefSeqGene project. On Dec 11, 2008 this sequence version replaced NM_006831.1. Transcript Variant: This variant (1) represents the longer transcript and encodes a longer protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: U73524.1, SRR1163658.309534.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGAAG AGGCTAATGA TGACAAGAAG CCAACCACTA AATTTGAACT AGAGCGAGAA 
ACAGAACTTC GCTTTGAGGT GGAGGCATCT CAGTCAGTTC AGTTGGAGTT GTTGACTGGC 
ATGGCAGAGA TCTTTGGCAC AGAGCTGACC CGAAACAAGA AATTCACCTT TGATGCTGGT 
GCCAAGGTGG CTGTTTTCAC TTGGCATGGC TGTTCTGTGC AACTGAGCGG CCGCACTGAG 
GTGGCTTATG TCTCCAAGGA CACTCCTATG TTGCTTTACC TCAACACTCA CACAGCCTTG 
GAACAGATGC GGAGGCAAGC GGAAAAGGAA GAAGAGCGAG GTCCCCGAGT GATGGTAGTG 
GGCCCCACTG ATGTGGGCAA GTCTACAGTG TGTCGCCTTC TGCTCAACTA CGCAGTGCGT 
TTGGGCCGCC GTCCCACTTA TGTGGAGCTG GATGTGGGCC AGGGTTCTGT GTCCATCCCT 
GGTACCATGG GGGCCCTCTA CATCGAGCGG CCTGCAGATG TCGAAGAGGG TTTCTCTATC 
CAGGCCCCTC TGGTGTATCA TTTTGGTTCC ACCACTCCTG GCACTAACAT CAAGCTTTAT 
AATAAGATTA CATCTCGTTT AGCAGATGTG TTCAACCAAA GGTGTGAGGT GAACCGAAGG 
GCATCTGTGA GTGGCTGTGT CATTAACACC TGTGGCTGGG TCAAGGGCTC TGGTTACCAG 
GCTCTGGTGC ATGCAGCCTC AGCTTTTGAG GTGGATGTCG TTGTTGTTCT GGATCAAGAA 
CGACTGTACA ATGAACTGAA ACGGGACCTC CCCCACTTTG TACGCACTGT GCTGCTCCCT 
AAATCTGGGG GTGTGGTGGA GCGCTCCAAG GACTTCCGGC GGGAATGTAG GGATGAGCGT 
ATCCGTGAGT ATTTTTATGG ATTCCGAGGC TGTTTCTATC CCCATGCCTT CAATGTCAAA 
TTTTCAGATG TGAAAATCTA CAAAGTTGGG GCACCCACCA TCCCAGACTC CTGTTTACCT 
TTGGGCATGT CTCAAGAGGA TAATCAGCTC AAGCTAGTAC CTGTCACTCC TGGGCGAGAT 
ATGGTGCACC ACCTACTGAG TGTTAGCACT GCCGAGGGTA CAGAGGAGAA CCTGTCCGAG 
ACAAGTGTAG CTGGCTTCAT TGTGGTGACC AGTGTGGACC TGGAGCATCA GGTGTTTACT 
GTTCTGTCTC CAGCCCCTCG CCCACTGCCT AAGAACTTCC TTCTCATCAT GGATATCCGG 
TTCATGGATC TGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006822.1
CDS	140..1417
Translation	MGEEANDDKKPTTKFELERETELRFEVEASQSVQLELLTGMAEIFGTELTRNKKFTFDAGAKVAVFTWHGCSVQLSGRTEVAYVSKDTPMLLYLNTHTALEQMRRQAEKEEERGPRVMVVGPTDVGKSTVCRLLLNYAVRLGRRPTYVELDVGQGSVSIPGTMGALYIERPADVEEGFSIQAPLVYHFGSTTPGTNIKLYNKITSRLADVFNQRCEVNRRASVSGCVINTCGWVKGSGYQALVHAASAFEVDVVVVLDQERLYNELKRDLPHFVRTVLLPKSGGVVERSKDFRRECRDERIREYFYGFRGCFYPHAFNVKFSDVKIYKVGAPTIPDSCLPLGMSQEDNQLKLVPVTPGRDMVHHLLSVSTAEGTEENLSETSVAGFIVVTSVDLEHQVFTVLSPAPRPLPKNFLLIMDIRFMDLK

Target ORF information:

RefSeq Version	NM_006831.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cleavage and polyadenylation factor I subunit 1 (CLP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006831.2

ORF Insert Sequence:

ATGGGAGAAG AGGCTAATGA TGACAAGAAG CCAACCACTA AATTTGAACT AGAGCGAGAA 
ACAGAACTTC GCTTTGAGGT GGAGGCATCT CAGTCAGTTC AGTTGGAGTT GTTGACTGGC 
ATGGCAGAGA TCTTTGGCAC AGAGCTGACC CGAAACAAGA AATTCACCTT TGATGCTGGT 
GCCAAGGTGG CTGTTTTCAC TTGGCATGGC TGTTCTGTGC AACTGAGCGG CCGCACTGAG 
GTGGCTTATG TCTCCAAGGA CACTCCTATG TTGCTTTACC TCAACACTCA CACAGCCTTG 
GAACAGATGC GGAGGCAAGC GGAAAAGGAA GAAGAGCGAG GTCCCCGAGT GATGGTAGTG 
GGCCCCACTG ATGTGGGCAA GTCTACAGTG TGTCGCCTTC TGCTCAACTA CGCAGTGCGT 
TTGGGCCGCC GTCCCACTTA TGTGGAGCTG GATGTGGGCC AGGGTTCTGT GTCCATCCCT 
GGTACCATGG GGGCCCTCTA CATCGAGCGG CCTGCAGATG TCGAAGAGGG TTTCTCTATC 
CAGGCCCCTC TGGTGTATCA TTTTGGTTCC ACCACTCCTG GCACTAACAT CAAGCTTTAT 
AATAAGATTA CATCTCGTTT AGCAGATGTG TTCAACCAAA GGTGTGAGGT GAACCGAAGG 
GCATCTGTGA GTGGCTGTGT CATTAACACC TGTGGCTGGG TCAAGGGCTC TGGTTACCAG 
GCTCTGGTGC ATGCAGCCTC AGCTTTTGAG GTGGATGTCG TTGTTGTTCT GGATCAAGAA 
CGACTGTACA ATGAACTGAA ACGGGACCTC CCCCACTTTG TACGCACTGT GCTGCTCCCT 
AAATCTGGGG GTGTGGTGGA GCGCTCCAAG GACTTCCGGC GGGAATGTAG GGATGAGCGT 
ATCCGTGAGT ATTTTTATGG ATTCCGAGGC TGTTTCTATC CCCATGCCTT CAATGTCAAA 
TTTTCAGATG TGAAAATCTA CAAAGTTGGG GCACCCACCA TCCCAGACTC CTGTTTACCT 
TTGGGCATGT CTCAAGAGGA TAATCAGCTC AAGCTAGTAC CTGTCACTCC TGGGCGAGAT 
ATGGTGCACC ACCTACTGAG TGTTAGCACT GCCGAGGGTA CAGAGGAGAA CCTGTCCGAG 
ACAAGTGTAG CTGGCTTCAT TGTGGTGACC AGTGTGGACC TGGAGCATCA GGTGTTTACT 
GTTCTGTCTC CAGCCCCTCG CCCACTGCCT AAGAACTTCC TTCTCATCAT GGATATCCGG 
TTCATGGATC TGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLP1 ( NM_001142597.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136069.1 Homo sapiens cleavage factor polyribonucleotide kinase subunit 1 (CLP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26511
Clone ID Related Accession (Same CDS sequence)	NM_001142597.1
Accession Version	NM_001142597.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	polyribonucleotide 5'-hydroxyl-kinase Clp1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA922553.1, AK300232.1 and BC000446.1. Transcript Variant: This variant (2) uses an alternate splice donor site and encodes a shorter protein (isoform 2) compared to transcript variant 1. ##Evidence-Data-START## Transcript exon combination :: AK300232.1, BG338247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGAAG AGGCTAATGA TGACAAGAAG CCAACCACTA AATTTGAACT AGAGCGAGAA 
ACAGAACTTC GCTTTGAGGT GGAGGCATCT CAGTCAGTTC AGTTGGAGTT GTTGACTGGC 
ATGGCAGAGA TCTTTGGCAC AGAGCTGACC CGAAACAAGA AATTCACCTT TGATGCTGGT 
GCCAAGGTGG CTGTTTTCAC TTGGCATGGC TGTTCTGTGC AACTGAGCGG CCGCACTGAG 
GTGGCTTATG TCTCCAAGGA CACTCCTATG TTGCTTTACC TCAACACTCA CACAGCCTTG 
GAACAGATGC GGAGGCAAGC GGAAAAGGAA GAAGAGCGAG GTCCCCGAGT GATGGTAGTG 
GGCCCCACTG ATGTGGGCAA GTCTACAGTG TGTCGCCTTC TGCTCAACTA CGCAATTACA 
TCTCGTTTAG CAGATGTGTT CAACCAAAGG TGTGAGGTGA ACCGAAGGGC ATCTGTGAGT 
GGCTGTGTCA TTAACACCTG TGGCTGGGTC AAGGGCTCTG GTTACCAGGC TCTGGTGCAT 
GCAGCCTCAG CTTTTGAGGT GGATGTCGTT GTTGTTCTGG ATCAAGAACG ACTGTACAAT 
GAACTGAAAC GGGACCTCCC CCACTTTGTA CGCACTGTGC TGCTCCCTAA ATCTGGGGGT 
GTGGTGGAGC GCTCCAAGGA CTTCCGGCGG GAATGTAGGG ATGAGCGTAT CCGTGAGTAT 
TTTTATGGAT TCCGAGGCTG TTTCTATCCC CATGCCTTCA ATGTCAAATT TTCAGATGTG 
AAAATCTACA AAGTTGGGGC ACCCACCATC CCAGACTCCT GTTTACCTTT GGGCATGTCT 
CAAGAGGATA ATCAGCTCAA GCTAGTACCT GTCACTCCTG GGCGAGATAT GGTGCACCAC 
CTACTGAGTG TTAGCACTGC CGAGGGTACA GAGGAGAACC TGTCCGAGAC AAGTGTAGCT 
GGCTTCATTG TGGTGACCAG TGTGGACCTG GAGCATCAGG TGTTTACTGT TCTGTCTCCA 
GCCCCTCGCC CACTGCCTAA GAACTTCCTT CTCATCATGG ATATCCGGTT CATGGATCTG 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136069.1
CDS	140..1225
Translation	MGEEANDDKKPTTKFELERETELRFEVEASQSVQLELLTGMAEIFGTELTRNKKFTFDAGAKVAVFTWHGCSVQLSGRTEVAYVSKDTPMLLYLNTHTALEQMRRQAEKEEERGPRVMVVGPTDVGKSTVCRLLLNYAITSRLADVFNQRCEVNRRASVSGCVINTCGWVKGSGYQALVHAASAFEVDVVVVLDQERLYNELKRDLPHFVRTVLLPKSGGVVERSKDFRRECRDERIREYFYGFRGCFYPHAFNVKFSDVKIYKVGAPTIPDSCLPLGMSQEDNQLKLVPVTPGRDMVHHLLSVSTAEGTEENLSETSVAGFIVVTSVDLEHQVFTVLSPAPRPLPKNFLLIMDIRFMDLK

Target ORF information:

RefSeq Version	NM_001142597.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cleavage factor polyribonucleotide kinase subunit 1 (CLP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142597.1

ORF Insert Sequence:

ATGGGAGAAG AGGCTAATGA TGACAAGAAG CCAACCACTA AATTTGAACT AGAGCGAGAA 
ACAGAACTTC GCTTTGAGGT GGAGGCATCT CAGTCAGTTC AGTTGGAGTT GTTGACTGGC 
ATGGCAGAGA TCTTTGGCAC AGAGCTGACC CGAAACAAGA AATTCACCTT TGATGCTGGT 
GCCAAGGTGG CTGTTTTCAC TTGGCATGGC TGTTCTGTGC AACTGAGCGG CCGCACTGAG 
GTGGCTTATG TCTCCAAGGA CACTCCTATG TTGCTTTACC TCAACACTCA CACAGCCTTG 
GAACAGATGC GGAGGCAAGC GGAAAAGGAA GAAGAGCGAG GTCCCCGAGT GATGGTAGTG 
GGCCCCACTG ATGTGGGCAA GTCTACAGTG TGTCGCCTTC TGCTCAACTA CGCAATTACA 
TCTCGTTTAG CAGATGTGTT CAACCAAAGG TGTGAGGTGA ACCGAAGGGC ATCTGTGAGT 
GGCTGTGTCA TTAACACCTG TGGCTGGGTC AAGGGCTCTG GTTACCAGGC TCTGGTGCAT 
GCAGCCTCAG CTTTTGAGGT GGATGTCGTT GTTGTTCTGG ATCAAGAACG ACTGTACAAT 
GAACTGAAAC GGGACCTCCC CCACTTTGTA CGCACTGTGC TGCTCCCTAA ATCTGGGGGT 
GTGGTGGAGC GCTCCAAGGA CTTCCGGCGG GAATGTAGGG ATGAGCGTAT CCGTGAGTAT 
TTTTATGGAT TCCGAGGCTG TTTCTATCCC CATGCCTTCA ATGTCAAATT TTCAGATGTG 
AAAATCTACA AAGTTGGGGC ACCCACCATC CCAGACTCCT GTTTACCTTT GGGCATGTCT 
CAAGAGGATA ATCAGCTCAA GCTAGTACCT GTCACTCCTG GGCGAGATAT GGTGCACCAC 
CTACTGAGTG TTAGCACTGC CGAGGGTACA GAGGAGAACC TGTCCGAGAC AAGTGTAGCT 
GGCTTCATTG TGGTGACCAG TGTGGACCTG GAGCATCAGG TGTTTACTGT TCTGTCTCCA 
GCCCCTCGCC CACTGCCTAA GAACTTCCTT CTCATCATGG ATATCCGGTT CATGGATCTG 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Biological insights from 108 schizophrenia-associated genetic loci.
Nature511(7510)421-7(2014 Jul)

Human CLP1 mutations alter tRNA biogenesis, affecting both peripheral and central nervous system function.
Cell157(3)636-50(2014 Apr)
Karaca E,Weitzer S,Pehlivan D,Shiraishi H,Gogakos T,Hanada T,Jhangiani SN,Wiszniewski W,Withers M,Campbell IM,Erdin S,Isikay S,Franco LM,Gonzaga-Jauregui C,Gambin T,Gelowani V,Hunter JV,Yesil G,Koparir E,Yilmaz S,Brown M,Briskin D,Hafner M,Morozov P,Farazi TA,Bernreuther C,Glatzel M,Trattnig S,Friske J,Kronnerwetter C,Bainbridge MN,Gezdirici A,Seven M,Muzny DM,Boerwinkle E,Ozen M,,Clausen T,Tuschl T,Yuksel A,Hess A,Gibbs RA,Martinez J,Penninger JM,Lupski JR

CLP1 founder mutation links tRNA splicing and maturation to cerebellar development and neurodegeneration.
Cell157(3)651-63(2014 Apr)
Schaffer AE,Eggens VR,Caglayan AO,Reuter MS,Scott E,Coufal NG,Silhavy JL,Xue Y,Kayserili H,Yasuno K,Rosti RO,Abdellateef M,Caglar C,Kasher PR,Cazemier JL,Weterman MA,Cantagrel V,Cai N,Zweier C,Altunoglu U,Satkin NB,Aktar F,Tuysuz B,Yalcinkaya C,Caksen H,Bilguvar K,Fu XD,Trotta CR,Gabriel S,Reis A,Gunel M,Baas F,Gleeson JG

Human RNA 5'-kinase (hClp1) can function as a tRNA splicing enzyme in vivo.
RNA (New York, N.Y.)14(9)1737-45(2008 Sep)
Ramirez A,Shuman S,Schwer B

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CLP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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