NFAT5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NFAT5
Entrez Gene ID	10725
Full Name	nuclear factor of activated T cells 5
Synonyms	NF-AT5,NFATL1,NFATZ,OREBP,TONEBP
General protein information	Preferred Names nuclear factor of activated T cells 5 Names nuclear factor of activated T-cells 5 NFAT-like protein 1 T-cell transcription factor NFAT5 TonE-binding protein glutamine rich protein H65 nuclear factor of activated T-cells 5, tonicity-responsive osmotic response element-binding protein tonicity-responsive enhancer-binding protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q22.1
Summary	The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	604708

mRNA and Protein(s)

mRNA	Protein	Name
XM_011522819.2	XP_011521121.1	nuclear factor of activated T-cells 5 isoform X5
XM_017022872.1	XP_016878361.1	nuclear factor of activated T-cells 5 isoform X5
XM_011522817.3	XP_011521119.1	nuclear factor of activated T-cells 5 isoform X1
XM_006721125.4	XP_006721188.1	nuclear factor of activated T-cells 5 isoform X2
XM_011522818.3	XP_011521120.1	nuclear factor of activated T-cells 5 isoform X3
XM_017022870.2	XP_016878359.1	nuclear factor of activated T-cells 5 isoform X3
XM_011522819.3	XP_011521121.1	nuclear factor of activated T-cells 5 isoform X5
NM_001367709.1	NP_001354638.1	nuclear factor of activated T-cells 5 isoform e
NM_173215.3	NP_775322.1	nuclear factor of activated T-cells 5 isoform a
NM_138713.4	NP_619727.2	nuclear factor of activated T-cells 5 isoform b
XM_017022870.1	XP_016878359.1	nuclear factor of activated T-cells 5 isoform X3
XM_011522820.2	XP_011521122.1	nuclear factor of activated T-cells 5 isoform X5
NM_001113178.2	NP_001106649.1	nuclear factor of activated T-cells 5 isoform d
NM_173214.3	NP_775321.1	nuclear factor of activated T-cells 5 isoform a
NM_138714.4	NP_619728.2	nuclear factor of activated T-cells 5 isoform a
XM_017022871.2	XP_016878360.1	nuclear factor of activated T-cells 5 isoform X4
XM_011522822.3	XP_011521124.1	nuclear factor of activated T-cells 5 isoform X5
NM_006599.4	NP_006590.1	nuclear factor of activated T-cells 5 isoform c
XM_011522818.2	XP_011521120.1	nuclear factor of activated T-cells 5 isoform X3
XM_011522822.2	XP_011521124.1	nuclear factor of activated T-cells 5 isoform X5
XM_017022872.2	XP_016878361.1	nuclear factor of activated T-cells 5 isoform X5
NM_001113178.3	NP_001106649.1	nuclear factor of activated T-cells 5 isoform d
XM_006721125.3	XP_006721188.1	nuclear factor of activated T-cells 5 isoform X2
XM_011522818.1	XP_011521120.1	nuclear factor of activated T-cells 5 isoform X1
NM_173214.2	NP_775321.1	nuclear factor of activated T-cells 5 isoform a
NM_006599.3	NP_006590.1	nuclear factor of activated T-cells 5 isoform c
XM_017022871.1	XP_016878360.1	nuclear factor of activated T-cells 5 isoform X4
NM_138714.3	NP_619728.2	nuclear factor of activated T-cells 5 isoform a
NM_173215.2	NP_775322.1	nuclear factor of activated T-cells 5 isoform a
XM_011522817.2	XP_011521119.1	nuclear factor of activated T-cells 5 isoform X1
NM_138713.3	NP_619727.2	nuclear factor of activated T-cells 5 isoform b
XM_017022873.1	XP_016878362.1	nuclear factor of activated T-cells 5 isoform X6
XM_011522820.3	XP_011521122.1	nuclear factor of activated T-cells 5 isoform X5

Pathways from BioSystems

Homologies

	NFAT5	XP_001093880.2
Mus musculus (house mouse)	Nfat5	NP_061293.2
Xenopus tropicalis (tropical clawed frog)	nfat5	XP_002937623.2
Homo sapiens (human)	NFAT5	NP_619727.2
Pan troglodytes (chimpanzee)	NFAT5	XP_001168930.2
Canis lupus familiaris (dog)	NFAT5	XP_005620824.1
Gallus gallus (chicken)	NFAT5	NP_001185929.1
Bos taurus (cattle)	NFAT5	XP_002694885.1
Rattus norvegicus (Norway rat)	Nfat5	NP_001100895.1
Danio rerio (zebrafish)	LOC555802	XP_005174610.1

Gene Ontology
Bibliography

Related articles in PubMed

Cleavage of osmosensitive transcriptional factor NFAT5 by Coxsackieviral protease 2A promotes viral replication.
Qiu Y, Ye X, Zhang HM, Hanson P, Zhao G, Tong L, Xie R, Yang D
PLoS pathogens13(12)e1006744(2017 Dec)

Tonicity-Responsive Enhancer-Binding Protein Mediates Hyperglycemia-Induced Inflammation and Vascular and Renal Injury.
Choi SY, Lim SW, Salimi S, Yoo EJ, Lee-Kwon W, Lee HH, Lee JH, Mitchell BD, Sanada S, Parsa A, Kwon HM
Journal of the American Society of Nephrology : JASN(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that protease 2A of CVB3 exhibits substrate specificity that includes human/mouse NFAT5 in cardiomyocytes; NFAT5 inhibits CVB3 replication via mechanism that involves iNOS; anti-CVB3 activity of NFAT5 is impaired during CVB3 infection due to protease 2A-mediated cleavage of NFAT5. (CVB3 = Coxsackievirus 3; NFAT5 = tonicity-responsive nuclear factor of activated T-cells 5; iNOS = nitric oxide synthase type II)
Title: Cleavage of osmosensitive transcriptional factor NFAT5 by Coxsackieviral protease 2A promotes viral replication.

TonEBP expression correlated with canonical osmoregulatory targets TauT/SLC6A6, SMIT/SLC5A3, and AR/AKR1B1, supporting in vitro findings that the inflammatory milieu during IDD does not interfere with TonEBP osmoregulation. In summary, whereas TonEBP participates in the proinflammatory response to TNF-alpha
Title: TNF-? promotes nuclear enrichment of the transcription factor TonEBP/NFAT5 to selectively control inflammatory but not osmoregulatory responses in nucleus pulposus cells.

genetic variation in NFAT5 expression and function in the central nervous system may affect the regulation of systemic water balance

results provide evidence that NFAT5 expression in macrophages enhances chronic arthritis by conferring apoptotic resistance to activated macrophages.
Title: Transcription factor NFAT5 promotes macrophage survival in rheumatoid arthritis.

Conclusion. miR-20b acts as a tumor suppressor in the development of thymoma and thymoma-associated myasthenia gravis. The tumor suppressive function of miR-20b in thymoma could be due to its inhibition of NFAT signaling by repression of NFAT5 and CAMTA1 expression.
Title: miR-20b Inhibits T Cell Proliferation and Activation via NFAT Signaling Pathway in Thymoma-Associated Myasthenia Gravis.

The following NFAT5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFAT5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10608	NM_138713.4 Latest version!	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu11639	NM_001113178.3 Latest version!	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu25124	NM_173215.3 Latest version!	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu25124	NM_173214.3 Latest version!	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu25124	NM_138714.4 Latest version!	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu25124	XM_011522818.3 Latest version!	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu25124	XM_017022870.2 Latest version!	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu11182	NM_006599.4 Latest version!	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$384.30 $549.00
OHu85815	NM_001367709.1 Latest version!	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu49247	XM_006721125.4 Latest version!	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu64333	XM_011522817.3 Latest version!	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu64334	XM_017022872.2 Latest version!	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu64334	XM_011522820.3 Latest version!	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu64334	XM_011522822.3 Latest version!	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu64334	XM_011522819.3 Latest version!	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu85814	XM_017022871.2 Latest version!	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu85815	XM_017022873.1 Latest version!	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OHu64334	XM_011522819.2	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu25124	XM_017022870.1	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu25124	XM_011522818.2	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu64334	XM_011522822.2	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu64334	XM_017022872.1	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu11639	NM_001113178.2	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu49247	XM_006721125.3	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu25124	XM_011522818.1	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu25124	NM_173214.2	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu11182	NM_006599.3	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$384.30 $549.00
OHu85814	XM_017022871.1	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu25124	NM_138714.3	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu25124	NM_173215.2	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu64333	XM_011522817.2	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu10608	NM_138713.3	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$384.30 $549.00
OHu64334	XM_011522820.2	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NFAT5 ( NM_138713.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_619727.2 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 2, mRNA.

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CloneID	OHu10608
Clone ID Related Accession (Same CDS sequence)	NM_138713.4 , NM_138713.3
Accession Version	NM_138713.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009032.8, AJ243299.1, BC131509.1, AC012321.8 and AA291524.1. This sequence is a reference standard in the RefSeqGene project. On Dec 12, 2018 this sequence version replaced NM_138713.3. Transcript Variant: This variant (2) lacks an exon in the 5' region, compared to variant 1. This difference causes translation initiation at an upstream start codon and results in an isoform (b) with a longer N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ243299.1, SRR1803615.141797.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000349945.7/ ENSP00000338806.3 RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTCTACTCGC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA 
TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA 
ACATCTGTAG CATCAATGAG TCAGACAAGC GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT 
GTTGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC 
TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT 
ATGCAAGTGG AGAGCTGCTC CTCAGCCGTG GGGGTAAGTA ACAGAGGGGT AAGTGAAAAG 
CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG 
TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG 
GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC 
TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA 
AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG 
GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG 
GACAACAAAG GCAACTCAAA AGCGGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA 
GTAAAGAAGA GCCCTATGTT GTGTGGACAA TATCCTGTTA AAAGTGAGGG AAAGGAGCTG 
AAGATAGTTG TACAACCTGA GACACAGCAC CGAGCTCGGT ACCTGACTGA GGGCAGCCGT 
GGCTCAGTGA AAGATAGAAC ACAGCAAGGC TTTCCTACAG TAAAGCTGGA AGGCCATAAT 
GAACCTGTAG TGTTGCAAGT GTTTGTGGGC AACGACTCTG GACGAGTGAA ACCACATGGA 
TTTTATCAGG CCTGCAGAGT AACTGGACGA AATACAACTC CTTGCAAAGA AGTGGACATT 
GAAGGCACTA CTGTTATAGA AGTCGGCCTT GATCCTAGCA ACAACATGAC ACTGGCGGTG 
GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTCG AAGCCAGAAT AGGAATTGCT 
GGTTCCAAGA AGAAAAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CATGAGGAAA 
GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTGTGTAC TCAGCCAGCA 
GGAGTGCCAG AAATCTTAAA GAAAAGCTTG CATAGCTGTT CAGTGAAAGG AGAAGAAGAA 
GTGTTTTTAA TCGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT 
TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAACTATT TCATCAGAAT 
CATCTTATTG TGAAGGTTCC TCCCTATCAT GACCAACATA TAACTTTGCC TGTGTCAGTG 
GGAATATATG TAGTGACAAA TGCTGGAAGA TCTCATGATG TTCAACCATT CACTTACACT 
CCAGACCCAG CAGCAGCTGG TGCTTTGAAT GTAAATGTGA AGAAGGAAAT ATCTAGTCCA 
GCAAGACCTT GCTCTTTTGA AGAGGCCATG AAAGCAATGA AAACTACTGG ATGTAATTTA 
GATAAGGTAA ATATTATCCC TAATGCCCTG ATGACTCCAC TCATACCAAG CAGTATGATT 
AAGAGTGAAG ATGTTACTCC AATGGAAGTA ACAGCAGAAA AAAGATCTTC CACTATTTTT 
AAGACTACAA AGTCTGTTGG ATCAACTCAG CAAACATTAG AAAACATCTC AAACATAGCA 
GGAAATGGCT CTTTTTCATC ACCATCATCT TCCCACCTAC CTTCTGAAAA TGAAAAACAG 
CAGCAGATTC AGCCCAAGGC ATACAACCCA GAGACCCTGA CAACTATTCA AACCCAGGAC 
ATCTCACAGC CTGGTACTTT TCCAGCAGTT TCTGCTTCTA GTCAGCTGCC CAACAGCGAT 
GCACTATTGC AGCAGGCTAC ACAGTTTCAG ACAAGAGAAA CTCAGTCTAG AGAGATATTA 
CAGTCAGATG GTACAGTGGT TAATTTGTCA CAACTGACTG AGGCATCACA ACAACAGCAG 
CAGTCACCAC TACAAGAACA AGCACAGACT TTACAGCAGC AGATTTCATC AAATATTTTT 
CCATCACCAA ATAGTGTGAG TCAGCTTCAG AATACTATTC AGCAGCTGCA AGCAGGGAGT 
TTCACAGGCA GTACTGCTAG TGGCAGCAGT GGAAGTGTTG ACTTGGTCCA ACAAGTTTTA 
GAGGCACAGC AGCAGTTATC TTCAGTTTTA TTTTCTGCTC CAGATGGTAA TGAGAATGTT 
CAAGAGCAGC TTAGTGCAGA TATTTTTCAA CAAGTCAGTC AAATTCAGAG TGGTGTAAGC 
CCTGGAATGT TTTCCTCAAC AGAGCCAACA GTCCATACCA GACCAGATAA TTTATTACCT 
GGAAGAGCTG AAAGTGTTCA TCCACAGTCT GAAAACACGT TATCTAATCA ACAGCAGCAG 
CAGCAGCAGC AACAGCAAGT GATGGAATCT TCAGCCGCAA TGGTGATGGA GATGCAACAG 
AGTATCTGCC AGGCAGCTGC CCAGATTCAG TCAGAGTTAT TCCCTTCAAC TGCTTCAGCA 
AATGGAAACC TTCAGCAATC GCCAGTTTAC CAGCAGACTT CTCACATGAT GAGTGCATTG 
TCTACCAATG AGGATATGCA AATGCAGTGT GAATTGTTTT CTTCTCCTCC TGCAGTTTCT 
GGAAATGAAA CTTCTACAAC TACCACACAG CAGGTTGCAA CCCCTGGCAC TACCATGTTT 
CAGACATCAA GTTCAGGAGA TGGAGAAGAA ACTGGAACAC AAGCAAAACA GATTCAGAAC 
AGTGTCTTTC AGACCATGGT CCAAATGCAA CATAGTGGGG ACAATCAACC TCAAGTTAAC 
CTTTTTTCAT CCACAAAAAG TATGATGAGT GTTCAGAATA GTGGTACCCA ACAACAAGGT 
AATGGTTTAT TCCAGCAAGG GAATGAGATG ATGTCACTTC AATCTGGAAA TTTTTTGCAG 
CAGTCTTCTC ATTCACAGGC CCAACTTTTT CATCCTCAAA ATCCTATTGC CGATGCTCAG 
AACCTTTCCC AGGAAACTCA AGGTTCTCTC TTTCATAGTC CAAATCCTAT TGTCCACAGT 
CAGACTTCTA CAACCTCCTC TGAACAAATG CAGCCTCCAA TGTTTCACTC TCAAAGTACC 
ATTGCTGTGT TACAGGGCTC TTCAGTTCCT CAAGACCAGC AGTCAACCAA CATATTTCTT 
TCCCAGAGTC CCATGAATAA TCTTCAGACT AACACAGTAG CCCAAGAAGC ATTTTTTGCA 
GCACCGAACT CAATTTCTCC ACTTCAGTCA ACATCAAACA GTGAACAACA AGCTGCTTTC 
CAACAGCAAG CTCCAATATC ACACATCCAG ACTCCTATGC TTTCCCAAGA ACAGGCACAA 
CCCCCGCAGC AGGGTTTATT TCAGCCTCAG GTGGCCCTGG GCTCCCTTCC ACCTAATCCA 
ATGCCTCAAA GCCAACAAGG AACCATGTTC CAGTCACAGC ACTCAATAGT TGCCATGCAG 
AGTAACTCTC CATCCCAGGA ACAGCAGCAG CAGCAGCAAC AGCAGCAGCA ACAGCAGCAG 
CAACAACAAC AGAGCATTTT ATTCAGTAAT CAGAATACCA TGGCTACAAT GGCGTCTCCA 
AAGCAACCAC CACCAAACAT GATATTCAAC CCAAATCAAA ATCCAATGGC TAATCAGGAG 
CAACAGAACC AGTCAATTTT TCACCAACAA AGTAACATGG CCCCAATGAA TCAAGAGCAA 
CAGCCCATGC AATTTCAGAG TCAGTCCACA GTTTCCTCAC TTCAGAACCC AGGTCCTACC 
CAGTCGGAAT CATCACAGAC CCCCTTGTTC CATAGCTCTC CTCAGATTCA GTTGGTACAA 
GGGTCACCTA GTTCTCAAGA GCAGCAAGTA ACTCTCTTCT TATCTCCAGC ATCCATGTCT 
GCCTTGCAGA CCAGTATAAA TCAACAAGAT ATGCAACAGT CTCCTCTTTA TTCCCCTCAG 
AACAACATGC CTGGAATTCA AGGAGCCACA TCTTCGCCTC AACCACAGGC TACTTTATTT 
CACAACACAG CAGGAGGCAC AATGAACCAA CTGCAGAATT CTCCTGGCTC ATCTCAGCAG 
ACATCAGGAA TGTTCTTATT TGGCATTCAA AATAACTGTA GTCAGCTTTT AACCTCTGGA 
CCAGCTACAT TGCCTGATCA GTTGATGGCC ATAAGTCAGC CAGGCCAACC ACAAAACGAG 
GGCCAGCCAC CTGTGACAAC ACTTCTTTCT CAGCAAATGC CAGAGAATTC TCCACTGGCA 
TCCTCTATAA ACACCAACCA GAACATCGAA AAGATTGATT TGCTTGTTTC ATTGCAAAAC 
CAAGGGAACA ACTTGACTGG CTCCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619727.2
CDS	337..4986
Translation	MPSDFISLLSADLDLESPKSLYSRDSLKLHPSQNFHRAGLLEESVYDLLPKELQLPPSRETSVASMSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	NM_138713.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138713.4

ORF Insert Sequence:

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTCTACTCGC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA 
TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA 
ACATCTGTAG CATCAATGAG TCAGACAAGC GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT 
GTTGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC 
TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT 
ATGCAAGTGG AGAGCTGCTC CTCAGCCGTG GGGGTAAGTA ACAGAGGGGT AAGTGAAAAG 
CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG 
TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG 
GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC 
TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA 
AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG 
GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG 
GACAACAAAG GCAACTCAAA AGCGGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA 
GTAAAGAAGA GCCCTATGTT GTGTGGACAA TATCCTGTTA AAAGTGAGGG AAAGGAGCTG 
AAGATAGTTG TACAACCTGA GACACAGCAC CGAGCTCGGT ACCTGACTGA GGGCAGCCGT 
GGCTCAGTGA AAGATAGAAC ACAGCAAGGC TTTCCTACAG TAAAGCTGGA AGGCCATAAT 
GAACCTGTAG TGTTGCAAGT GTTTGTGGGC AACGACTCTG GACGAGTGAA ACCACATGGA 
TTTTATCAGG CCTGCAGAGT AACTGGACGA AATACAACTC CTTGCAAAGA AGTGGACATT 
GAAGGCACTA CTGTTATAGA AGTCGGCCTT GATCCTAGCA ACAACATGAC ACTGGCGGTG 
GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTCG AAGCCAGAAT AGGAATTGCT 
GGTTCCAAGA AGAAAAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CATGAGGAAA 
GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTGTGTAC TCAGCCAGCA 
GGAGTGCCAG AAATCTTAAA GAAAAGCTTG CATAGCTGTT CAGTGAAAGG AGAAGAAGAA 
GTGTTTTTAA TCGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT 
TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAACTATT TCATCAGAAT 
CATCTTATTG TGAAGGTTCC TCCCTATCAT GACCAACATA TAACTTTGCC TGTGTCAGTG 
GGAATATATG TAGTGACAAA TGCTGGAAGA TCTCATGATG TTCAACCATT CACTTACACT 
CCAGACCCAG CAGCAGCTGG TGCTTTGAAT GTAAATGTGA AGAAGGAAAT ATCTAGTCCA 
GCAAGACCTT GCTCTTTTGA AGAGGCCATG AAAGCAATGA AAACTACTGG ATGTAATTTA 
GATAAGGTAA ATATTATCCC TAATGCCCTG ATGACTCCAC TCATACCAAG CAGTATGATT 
AAGAGTGAAG ATGTTACTCC AATGGAAGTA ACAGCAGAAA AAAGATCTTC CACTATTTTT 
AAGACTACAA AGTCTGTTGG ATCAACTCAG CAAACATTAG AAAACATCTC AAACATAGCA 
GGAAATGGCT CTTTTTCATC ACCATCATCT TCCCACCTAC CTTCTGAAAA TGAAAAACAG 
CAGCAGATTC AGCCCAAGGC ATACAACCCA GAGACCCTGA CAACTATTCA AACCCAGGAC 
ATCTCACAGC CTGGTACTTT TCCAGCAGTT TCTGCTTCTA GTCAGCTGCC CAACAGCGAT 
GCACTATTGC AGCAGGCTAC ACAGTTTCAG ACAAGAGAAA CTCAGTCTAG AGAGATATTA 
CAGTCAGATG GTACAGTGGT TAATTTGTCA CAACTGACTG AGGCATCACA ACAACAGCAG 
CAGTCACCAC TACAAGAACA AGCACAGACT TTACAGCAGC AGATTTCATC AAATATTTTT 
CCATCACCAA ATAGTGTGAG TCAGCTTCAG AATACTATTC AGCAGCTGCA AGCAGGGAGT 
TTCACAGGCA GTACTGCTAG TGGCAGCAGT GGAAGTGTTG ACTTGGTCCA ACAAGTTTTA 
GAGGCACAGC AGCAGTTATC TTCAGTTTTA TTTTCTGCTC CAGATGGTAA TGAGAATGTT 
CAAGAGCAGC TTAGTGCAGA TATTTTTCAA CAAGTCAGTC AAATTCAGAG TGGTGTAAGC 
CCTGGAATGT TTTCCTCAAC AGAGCCAACA GTCCATACCA GACCAGATAA TTTATTACCT 
GGAAGAGCTG AAAGTGTTCA TCCACAGTCT GAAAACACGT TATCTAATCA ACAGCAGCAG 
CAGCAGCAGC AACAGCAAGT GATGGAATCT TCAGCCGCAA TGGTGATGGA GATGCAACAG 
AGTATCTGCC AGGCAGCTGC CCAGATTCAG TCAGAGTTAT TCCCTTCAAC TGCTTCAGCA 
AATGGAAACC TTCAGCAATC GCCAGTTTAC CAGCAGACTT CTCACATGAT GAGTGCATTG 
TCTACCAATG AGGATATGCA AATGCAGTGT GAATTGTTTT CTTCTCCTCC TGCAGTTTCT 
GGAAATGAAA CTTCTACAAC TACCACACAG CAGGTTGCAA CCCCTGGCAC TACCATGTTT 
CAGACATCAA GTTCAGGAGA TGGAGAAGAA ACTGGAACAC AAGCAAAACA GATTCAGAAC 
AGTGTCTTTC AGACCATGGT CCAAATGCAA CATAGTGGGG ACAATCAACC TCAAGTTAAC 
CTTTTTTCAT CCACAAAAAG TATGATGAGT GTTCAGAATA GTGGTACCCA ACAACAAGGT 
AATGGTTTAT TCCAGCAAGG GAATGAGATG ATGTCACTTC AATCTGGAAA TTTTTTGCAG 
CAGTCTTCTC ATTCACAGGC CCAACTTTTT CATCCTCAAA ATCCTATTGC CGATGCTCAG 
AACCTTTCCC AGGAAACTCA AGGTTCTCTC TTTCATAGTC CAAATCCTAT TGTCCACAGT 
CAGACTTCTA CAACCTCCTC TGAACAAATG CAGCCTCCAA TGTTTCACTC TCAAAGTACC 
ATTGCTGTGT TACAGGGCTC TTCAGTTCCT CAAGACCAGC AGTCAACCAA CATATTTCTT 
TCCCAGAGTC CCATGAATAA TCTTCAGACT AACACAGTAG CCCAAGAAGC ATTTTTTGCA 
GCACCGAACT CAATTTCTCC ACTTCAGTCA ACATCAAACA GTGAACAACA AGCTGCTTTC 
CAACAGCAAG CTCCAATATC ACACATCCAG ACTCCTATGC TTTCCCAAGA ACAGGCACAA 
CCCCCGCAGC AGGGTTTATT TCAGCCTCAG GTGGCCCTGG GCTCCCTTCC ACCTAATCCA 
ATGCCTCAAA GCCAACAAGG AACCATGTTC CAGTCACAGC ACTCAATAGT TGCCATGCAG 
AGTAACTCTC CATCCCAGGA ACAGCAGCAG CAGCAGCAAC AGCAGCAGCA ACAGCAGCAG 
CAACAACAAC AGAGCATTTT ATTCAGTAAT CAGAATACCA TGGCTACAAT GGCGTCTCCA 
AAGCAACCAC CACCAAACAT GATATTCAAC CCAAATCAAA ATCCAATGGC TAATCAGGAG 
CAACAGAACC AGTCAATTTT TCACCAACAA AGTAACATGG CCCCAATGAA TCAAGAGCAA 
CAGCCCATGC AATTTCAGAG TCAGTCCACA GTTTCCTCAC TTCAGAACCC AGGTCCTACC 
CAGTCGGAAT CATCACAGAC CCCCTTGTTC CATAGCTCTC CTCAGATTCA GTTGGTACAA 
GGGTCACCTA GTTCTCAAGA GCAGCAAGTA ACTCTCTTCT TATCTCCAGC ATCCATGTCT 
GCCTTGCAGA CCAGTATAAA TCAACAAGAT ATGCAACAGT CTCCTCTTTA TTCCCCTCAG 
AACAACATGC CTGGAATTCA AGGAGCCACA TCTTCGCCTC AACCACAGGC TACTTTATTT 
CACAACACAG CAGGAGGCAC AATGAACCAA CTGCAGAATT CTCCTGGCTC ATCTCAGCAG 
ACATCAGGAA TGTTCTTATT TGGCATTCAA AATAACTGTA GTCAGCTTTT AACCTCTGGA 
CCAGCTACAT TGCCTGATCA GTTGATGGCC ATAAGTCAGC CAGGCCAACC ACAAAACGAG 
GGCCAGCCAC CTGTGACAAC ACTTCTTTCT CAGCAAATGC CAGAGAATTC TCCACTGGCA 
TCCTCTATAA ACACCAACCA GAACATCGAA AAGATTGATT TGCTTGTTTC ATTGCAAAAC 
CAAGGGAACA ACTTGACTGG CTCCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( NM_001113178.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001106649.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 6, mRNA.

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CloneID	OHu11639
Clone ID Related Accession (Same CDS sequence)	NM_001113178.2 , NM_001113178.3
Accession Version	NM_001113178.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009032.8, BC131509.1, AC012321.8 and AA291524.1. On Aug 4, 2011 this sequence version replaced NM_001113178.1. Transcript Variant: This variant (6) lacks an exon in the 5' region and uses an alternate in-frame splice site in the central coding region, compared to variant 1. These differences result in an isoform (d) with a longer N-terminus and lacking an internal aa, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC131509.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTCTACTCGC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA 
TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA 
ACATCTGTAG CATCAATGAG TCAGACAAGC GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT 
GTTGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC 
TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT 
ATGCAAGTGG AGAGCTGCTC CTCAGCCGTG GGGGTAAGTA ACAGAGGGGT AAGTGAAAAG 
CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG 
TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG 
GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC 
TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA 
AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG 
GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG 
GACAACAAAG GCAACTCAAA AGCGGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA 
GTAAAGAAGA GCCCTATGTT GTGTGGACAA TATCCTGTTA AAAGTGAGGG AAAGGAGCTG 
AAGATAGTTG TACAACCTGA GACACAGCAC CGAGCTCGGT ACCTGACTGA GGGCAGCCGT 
GGCTCAGTGA AAGATAGAAC ACAGCAAGGC TTTCCTACAG TAAAGCTGGA AGGCCATAAT 
GAACCTGTAG TGTTGCAAGT GTTTGTGGGC AACGACTCTG GACGAGTGAA ACCACATGGA 
TTTTATCAGG CCTGCAGAGT AACTGGACGA AATACAACTC CTTGCAAAGA AGTGGACATT 
GAAGGCACTA CTGTTATAGA AGTCGGCCTT GATCCTAGCA ACAACATGAC ACTGGCGGTG 
GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTCG AAGCCAGAAT AGGAATTGCT 
GGTTCCAAGA AGAAAAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CATGAGGAAA 
GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTGTGTAC TCAGCCAGCA 
GGAGTGCCAG AAATCTTAAA GAAAAGCTTG CATAGCTGTT CAGTGAAAGG AGAAGAAGAA 
GTGTTTTTAA TCGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT 
TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAACTATT TCATCAGAAT 
CATCTTATTG TGAAGGTTCC TCCCTATCAT GACCAACATA TAACTTTGCC TGTGTCAGTG 
GGAATATATG TAGTGACAAA TGCTGGAAGA TCTCATGATG TTCAACCATT CACTTACACT 
CCAGACCCAG CAGCTGGTGC TTTGAATGTA AATGTGAAGA AGGAAATATC TAGTCCAGCA 
AGACCTTGCT CTTTTGAAGA GGCCATGAAA GCAATGAAAA CTACTGGATG TAATTTAGAT 
AAGGTAAATA TTATCCCTAA TGCCCTGATG ACTCCACTCA TACCAAGCAG TATGATTAAG 
AGTGAAGATG TTACTCCAAT GGAAGTAACA GCAGAAAAAA GATCTTCCAC TATTTTTAAG 
ACTACAAAGT CTGTTGGATC AACTCAGCAA ACATTAGAAA ACATCTCAAA CATAGCAGGA 
AATGGCTCTT TTTCATCACC ATCATCTTCC CACCTACCTT CTGAAAATGA AAAACAGCAG 
CAGATTCAGC CCAAGGCATA CAACCCAGAG ACCCTGACAA CTATTCAAAC CCAGGACATC 
TCACAGCCTG GTACTTTTCC AGCAGTTTCT GCTTCTAGTC AGCTGCCCAA CAGCGATGCA 
CTATTGCAGC AGGCTACACA GTTTCAGACA AGAGAAACTC AGTCTAGAGA GATATTACAG 
TCAGATGGTA CAGTGGTTAA TTTGTCACAA CTGACTGAGG CATCACAACA ACAGCAGCAG 
TCACCACTAC AAGAACAAGC ACAGACTTTA CAGCAGCAGA TTTCATCAAA TATTTTTCCA 
TCACCAAATA GTGTGAGTCA GCTTCAGAAT ACTATTCAGC AGCTGCAAGC AGGGAGTTTC 
ACAGGCAGTA CTGCTAGTGG CAGCAGTGGA AGTGTTGACT TGGTCCAACA AGTTTTAGAG 
GCACAGCAGC AGTTATCTTC AGTTTTATTT TCTGCTCCAG ATGGTAATGA GAATGTTCAA 
GAGCAGCTTA GTGCAGATAT TTTTCAACAA GTCAGTCAAA TTCAGAGTGG TGTAAGCCCT 
GGAATGTTTT CCTCAACAGA GCCAACAGTC CATACCAGAC CAGATAATTT ATTACCTGGA 
AGAGCTGAAA GTGTTCATCC ACAGTCTGAA AACACGTTAT CTAATCAACA GCAGCAGCAG 
CAGCAGCAAC AGCAAGTGAT GGAATCTTCA GCCGCAATGG TGATGGAGAT GCAACAGAGT 
ATCTGCCAGG CAGCTGCCCA GATTCAGTCA GAGTTATTCC CTTCAACTGC TTCAGCAAAT 
GGAAACCTTC AGCAATCGCC AGTTTACCAG CAGACTTCTC ACATGATGAG TGCATTGTCT 
ACCAATGAGG ATATGCAAAT GCAGTGTGAA TTGTTTTCTT CTCCTCCTGC AGTTTCTGGA 
AATGAAACTT CTACAACTAC CACACAGCAG GTTGCAACCC CTGGCACTAC CATGTTTCAG 
ACATCAAGTT CAGGAGATGG AGAAGAAACT GGAACACAAG CAAAACAGAT TCAGAACAGT 
GTCTTTCAGA CCATGGTCCA AATGCAACAT AGTGGGGACA ATCAACCTCA AGTTAACCTT 
TTTTCATCCA CAAAAAGTAT GATGAGTGTT CAGAATAGTG GTACCCAACA ACAAGGTAAT 
GGTTTATTCC AGCAAGGGAA TGAGATGATG TCACTTCAAT CTGGAAATTT TTTGCAGCAG 
TCTTCTCATT CACAGGCCCA ACTTTTTCAT CCTCAAAATC CTATTGCCGA TGCTCAGAAC 
CTTTCCCAGG AAACTCAAGG TTCTCTCTTT CATAGTCCAA ATCCTATTGT CCACAGTCAG 
ACTTCTACAA CCTCCTCTGA ACAAATGCAG CCTCCAATGT TTCACTCTCA AAGTACCATT 
GCTGTGTTAC AGGGCTCTTC AGTTCCTCAA GACCAGCAGT CAACCAACAT ATTTCTTTCC 
CAGAGTCCCA TGAATAATCT TCAGACTAAC ACAGTAGCCC AAGAAGCATT TTTTGCAGCA 
CCGAACTCAA TTTCTCCACT TCAGTCAACA TCAAACAGTG AACAACAAGC TGCTTTCCAA 
CAGCAAGCTC CAATATCACA CATCCAGACT CCTATGCTTT CCCAAGAACA GGCACAACCC 
CCGCAGCAGG GTTTATTTCA GCCTCAGGTG GCCCTGGGCT CCCTTCCACC TAATCCAATG 
CCTCAAAGCC AACAAGGAAC CATGTTCCAG TCACAGCACT CAATAGTTGC CATGCAGAGT 
AACTCTCCAT CCCAGGAACA GCAGCAGCAG CAGCAACAGC AGCAGCAACA GCAGCAGCAA 
CAACAACAGA GCATTTTATT CAGTAATCAG AATACCATGG CTACAATGGC GTCTCCAAAG 
CAACCACCAC CAAACATGAT ATTCAACCCA AATCAAAATC CAATGGCTAA TCAGGAGCAA 
CAGAACCAGT CAATTTTTCA CCAACAAAGT AACATGGCCC CAATGAATCA AGAGCAACAG 
CCCATGCAAT TTCAGAGTCA GTCCACAGTT TCCTCACTTC AGAACCCAGG TCCTACCCAG 
TCGGAATCAT CACAGACCCC CTTGTTCCAT AGCTCTCCTC AGATTCAGTT GGTACAAGGG 
TCACCTAGTT CTCAAGAGCA GCAAGTAACT CTCTTCTTAT CTCCAGCATC CATGTCTGCC 
TTGCAGACCA GTATAAATCA ACAAGATATG CAACAGTCTC CTCTTTATTC CCCTCAGAAC 
AACATGCCTG GAATTCAAGG AGCCACATCT TCGCCTCAAC CACAGGCTAC TTTATTTCAC 
AACACAGCAG GAGGCACAAT GAACCAACTG CAGAATTCTC CTGGCTCATC TCAGCAGACA 
TCAGGAATGT TCTTATTTGG CATTCAAAAT AACTGTAGTC AGCTTTTAAC CTCTGGACCA 
GCTACATTGC CTGATCAGTT GATGGCCATA AGTCAGCCAG GCCAACCACA AAACGAGGGC 
CAGCCACCTG TGACAACACT TCTTTCTCAG CAAATGCCAG AGAATTCTCC ACTGGCATCC 
TCTATAAACA CCAACCAGAA CATCGAAAAG ATTGATTTGC TTGTTTCATT GCAAAACCAA 
GGGAACAACT TGACTGGCTC CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106649.1
CDS	337..4983
Translation	MPSDFISLLSADLDLESPKSLYSRDSLKLHPSQNFHRAGLLEESVYDLLPKELQLPPSRETSVASMSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	NM_001113178.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113178.2

ORF Insert Sequence:

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTCTACTCGC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA 
TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA 
ACATCTGTAG CATCAATGAG TCAGACAAGC GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT 
GTTGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC 
TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT 
ATGCAAGTGG AGAGCTGCTC CTCAGCCGTG GGGGTAAGTA ACAGAGGGGT AAGTGAAAAG 
CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG 
TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG 
GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC 
TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA 
AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG 
GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG 
GACAACAAAG GCAACTCAAA AGCGGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA 
GTAAAGAAGA GCCCTATGTT GTGTGGACAA TATCCTGTTA AAAGTGAGGG AAAGGAGCTG 
AAGATAGTTG TACAACCTGA GACACAGCAC CGAGCTCGGT ACCTGACTGA GGGCAGCCGT 
GGCTCAGTGA AAGATAGAAC ACAGCAAGGC TTTCCTACAG TAAAGCTGGA AGGCCATAAT 
GAACCTGTAG TGTTGCAAGT GTTTGTGGGC AACGACTCTG GACGAGTGAA ACCACATGGA 
TTTTATCAGG CCTGCAGAGT AACTGGACGA AATACAACTC CTTGCAAAGA AGTGGACATT 
GAAGGCACTA CTGTTATAGA AGTCGGCCTT GATCCTAGCA ACAACATGAC ACTGGCGGTG 
GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTCG AAGCCAGAAT AGGAATTGCT 
GGTTCCAAGA AGAAAAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CATGAGGAAA 
GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTGTGTAC TCAGCCAGCA 
GGAGTGCCAG AAATCTTAAA GAAAAGCTTG CATAGCTGTT CAGTGAAAGG AGAAGAAGAA 
GTGTTTTTAA TCGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT 
TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAACTATT TCATCAGAAT 
CATCTTATTG TGAAGGTTCC TCCCTATCAT GACCAACATA TAACTTTGCC TGTGTCAGTG 
GGAATATATG TAGTGACAAA TGCTGGAAGA TCTCATGATG TTCAACCATT CACTTACACT 
CCAGACCCAG CAGCTGGTGC TTTGAATGTA AATGTGAAGA AGGAAATATC TAGTCCAGCA 
AGACCTTGCT CTTTTGAAGA GGCCATGAAA GCAATGAAAA CTACTGGATG TAATTTAGAT 
AAGGTAAATA TTATCCCTAA TGCCCTGATG ACTCCACTCA TACCAAGCAG TATGATTAAG 
AGTGAAGATG TTACTCCAAT GGAAGTAACA GCAGAAAAAA GATCTTCCAC TATTTTTAAG 
ACTACAAAGT CTGTTGGATC AACTCAGCAA ACATTAGAAA ACATCTCAAA CATAGCAGGA 
AATGGCTCTT TTTCATCACC ATCATCTTCC CACCTACCTT CTGAAAATGA AAAACAGCAG 
CAGATTCAGC CCAAGGCATA CAACCCAGAG ACCCTGACAA CTATTCAAAC CCAGGACATC 
TCACAGCCTG GTACTTTTCC AGCAGTTTCT GCTTCTAGTC AGCTGCCCAA CAGCGATGCA 
CTATTGCAGC AGGCTACACA GTTTCAGACA AGAGAAACTC AGTCTAGAGA GATATTACAG 
TCAGATGGTA CAGTGGTTAA TTTGTCACAA CTGACTGAGG CATCACAACA ACAGCAGCAG 
TCACCACTAC AAGAACAAGC ACAGACTTTA CAGCAGCAGA TTTCATCAAA TATTTTTCCA 
TCACCAAATA GTGTGAGTCA GCTTCAGAAT ACTATTCAGC AGCTGCAAGC AGGGAGTTTC 
ACAGGCAGTA CTGCTAGTGG CAGCAGTGGA AGTGTTGACT TGGTCCAACA AGTTTTAGAG 
GCACAGCAGC AGTTATCTTC AGTTTTATTT TCTGCTCCAG ATGGTAATGA GAATGTTCAA 
GAGCAGCTTA GTGCAGATAT TTTTCAACAA GTCAGTCAAA TTCAGAGTGG TGTAAGCCCT 
GGAATGTTTT CCTCAACAGA GCCAACAGTC CATACCAGAC CAGATAATTT ATTACCTGGA 
AGAGCTGAAA GTGTTCATCC ACAGTCTGAA AACACGTTAT CTAATCAACA GCAGCAGCAG 
CAGCAGCAAC AGCAAGTGAT GGAATCTTCA GCCGCAATGG TGATGGAGAT GCAACAGAGT 
ATCTGCCAGG CAGCTGCCCA GATTCAGTCA GAGTTATTCC CTTCAACTGC TTCAGCAAAT 
GGAAACCTTC AGCAATCGCC AGTTTACCAG CAGACTTCTC ACATGATGAG TGCATTGTCT 
ACCAATGAGG ATATGCAAAT GCAGTGTGAA TTGTTTTCTT CTCCTCCTGC AGTTTCTGGA 
AATGAAACTT CTACAACTAC CACACAGCAG GTTGCAACCC CTGGCACTAC CATGTTTCAG 
ACATCAAGTT CAGGAGATGG AGAAGAAACT GGAACACAAG CAAAACAGAT TCAGAACAGT 
GTCTTTCAGA CCATGGTCCA AATGCAACAT AGTGGGGACA ATCAACCTCA AGTTAACCTT 
TTTTCATCCA CAAAAAGTAT GATGAGTGTT CAGAATAGTG GTACCCAACA ACAAGGTAAT 
GGTTTATTCC AGCAAGGGAA TGAGATGATG TCACTTCAAT CTGGAAATTT TTTGCAGCAG 
TCTTCTCATT CACAGGCCCA ACTTTTTCAT CCTCAAAATC CTATTGCCGA TGCTCAGAAC 
CTTTCCCAGG AAACTCAAGG TTCTCTCTTT CATAGTCCAA ATCCTATTGT CCACAGTCAG 
ACTTCTACAA CCTCCTCTGA ACAAATGCAG CCTCCAATGT TTCACTCTCA AAGTACCATT 
GCTGTGTTAC AGGGCTCTTC AGTTCCTCAA GACCAGCAGT CAACCAACAT ATTTCTTTCC 
CAGAGTCCCA TGAATAATCT TCAGACTAAC ACAGTAGCCC AAGAAGCATT TTTTGCAGCA 
CCGAACTCAA TTTCTCCACT TCAGTCAACA TCAAACAGTG AACAACAAGC TGCTTTCCAA 
CAGCAAGCTC CAATATCACA CATCCAGACT CCTATGCTTT CCCAAGAACA GGCACAACCC 
CCGCAGCAGG GTTTATTTCA GCCTCAGGTG GCCCTGGGCT CCCTTCCACC TAATCCAATG 
CCTCAAAGCC AACAAGGAAC CATGTTCCAG TCACAGCACT CAATAGTTGC CATGCAGAGT 
AACTCTCCAT CCCAGGAACA GCAGCAGCAG CAGCAACAGC AGCAGCAACA GCAGCAGCAA 
CAACAACAGA GCATTTTATT CAGTAATCAG AATACCATGG CTACAATGGC GTCTCCAAAG 
CAACCACCAC CAAACATGAT ATTCAACCCA AATCAAAATC CAATGGCTAA TCAGGAGCAA 
CAGAACCAGT CAATTTTTCA CCAACAAAGT AACATGGCCC CAATGAATCA AGAGCAACAG 
CCCATGCAAT TTCAGAGTCA GTCCACAGTT TCCTCACTTC AGAACCCAGG TCCTACCCAG 
TCGGAATCAT CACAGACCCC CTTGTTCCAT AGCTCTCCTC AGATTCAGTT GGTACAAGGG 
TCACCTAGTT CTCAAGAGCA GCAAGTAACT CTCTTCTTAT CTCCAGCATC CATGTCTGCC 
TTGCAGACCA GTATAAATCA ACAAGATATG CAACAGTCTC CTCTTTATTC CCCTCAGAAC 
AACATGCCTG GAATTCAAGG AGCCACATCT TCGCCTCAAC CACAGGCTAC TTTATTTCAC 
AACACAGCAG GAGGCACAAT GAACCAACTG CAGAATTCTC CTGGCTCATC TCAGCAGACA 
TCAGGAATGT TCTTATTTGG CATTCAAAAT AACTGTAGTC AGCTTTTAAC CTCTGGACCA 
GCTACATTGC CTGATCAGTT GATGGCCATA AGTCAGCCAG GCCAACCACA AAACGAGGGC 
CAGCCACCTG TGACAACACT TCTTTCTCAG CAAATGCCAG AGAATTCTCC ACTGGCATCC 
TCTATAAACA CCAACCAGAA CATCGAAAAG ATTGATTTGC TTGTTTCATT GCAAAACCAA 
GGGAACAACT TGACTGGCTC CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( NM_001113178.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001106649.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 6, mRNA.

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CloneID	OHu11639
Clone ID Related Accession (Same CDS sequence)	NM_001113178.2 , NM_001113178.3
Accession Version	NM_001113178.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009032.8, BC131509.1, AC012321.8 and AA291524.1. On May 31, 2019 this sequence version replaced NM_001113178.2. Transcript Variant: This variant (6) lacks an exon in the 5' region and uses an alternate in-frame splice site in the central coding region, compared to variant 1. These differences result in an isoform (d) with a longer N-terminus and lacking an internal aa, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC131509.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTCTACTCGC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA 
TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA 
ACATCTGTAG CATCAATGAG TCAGACAAGC GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT 
GTTGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC 
TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT 
ATGCAAGTGG AGAGCTGCTC CTCAGCCGTG GGGGTAAGTA ACAGAGGGGT AAGTGAAAAG 
CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG 
TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG 
GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC 
TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA 
AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG 
GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG 
GACAACAAAG GCAACTCAAA AGCGGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA 
GTAAAGAAGA GCCCTATGTT GTGTGGACAA TATCCTGTTA AAAGTGAGGG AAAGGAGCTG 
AAGATAGTTG TACAACCTGA GACACAGCAC CGAGCTCGGT ACCTGACTGA GGGCAGCCGT 
GGCTCAGTGA AAGATAGAAC ACAGCAAGGC TTTCCTACAG TAAAGCTGGA AGGCCATAAT 
GAACCTGTAG TGTTGCAAGT GTTTGTGGGC AACGACTCTG GACGAGTGAA ACCACATGGA 
TTTTATCAGG CCTGCAGAGT AACTGGACGA AATACAACTC CTTGCAAAGA AGTGGACATT 
GAAGGCACTA CTGTTATAGA AGTCGGCCTT GATCCTAGCA ACAACATGAC ACTGGCGGTG 
GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTCG AAGCCAGAAT AGGAATTGCT 
GGTTCCAAGA AGAAAAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CATGAGGAAA 
GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTGTGTAC TCAGCCAGCA 
GGAGTGCCAG AAATCTTAAA GAAAAGCTTG CATAGCTGTT CAGTGAAAGG AGAAGAAGAA 
GTGTTTTTAA TCGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT 
TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAACTATT TCATCAGAAT 
CATCTTATTG TGAAGGTTCC TCCCTATCAT GACCAACATA TAACTTTGCC TGTGTCAGTG 
GGAATATATG TAGTGACAAA TGCTGGAAGA TCTCATGATG TTCAACCATT CACTTACACT 
CCAGACCCAG CAGCTGGTGC TTTGAATGTA AATGTGAAGA AGGAAATATC TAGTCCAGCA 
AGACCTTGCT CTTTTGAAGA GGCCATGAAA GCAATGAAAA CTACTGGATG TAATTTAGAT 
AAGGTAAATA TTATCCCTAA TGCCCTGATG ACTCCACTCA TACCAAGCAG TATGATTAAG 
AGTGAAGATG TTACTCCAAT GGAAGTAACA GCAGAAAAAA GATCTTCCAC TATTTTTAAG 
ACTACAAAGT CTGTTGGATC AACTCAGCAA ACATTAGAAA ACATCTCAAA CATAGCAGGA 
AATGGCTCTT TTTCATCACC ATCATCTTCC CACCTACCTT CTGAAAATGA AAAACAGCAG 
CAGATTCAGC CCAAGGCATA CAACCCAGAG ACCCTGACAA CTATTCAAAC CCAGGACATC 
TCACAGCCTG GTACTTTTCC AGCAGTTTCT GCTTCTAGTC AGCTGCCCAA CAGCGATGCA 
CTATTGCAGC AGGCTACACA GTTTCAGACA AGAGAAACTC AGTCTAGAGA GATATTACAG 
TCAGATGGTA CAGTGGTTAA TTTGTCACAA CTGACTGAGG CATCACAACA ACAGCAGCAG 
TCACCACTAC AAGAACAAGC ACAGACTTTA CAGCAGCAGA TTTCATCAAA TATTTTTCCA 
TCACCAAATA GTGTGAGTCA GCTTCAGAAT ACTATTCAGC AGCTGCAAGC AGGGAGTTTC 
ACAGGCAGTA CTGCTAGTGG CAGCAGTGGA AGTGTTGACT TGGTCCAACA AGTTTTAGAG 
GCACAGCAGC AGTTATCTTC AGTTTTATTT TCTGCTCCAG ATGGTAATGA GAATGTTCAA 
GAGCAGCTTA GTGCAGATAT TTTTCAACAA GTCAGTCAAA TTCAGAGTGG TGTAAGCCCT 
GGAATGTTTT CCTCAACAGA GCCAACAGTC CATACCAGAC CAGATAATTT ATTACCTGGA 
AGAGCTGAAA GTGTTCATCC ACAGTCTGAA AACACGTTAT CTAATCAACA GCAGCAGCAG 
CAGCAGCAAC AGCAAGTGAT GGAATCTTCA GCCGCAATGG TGATGGAGAT GCAACAGAGT 
ATCTGCCAGG CAGCTGCCCA GATTCAGTCA GAGTTATTCC CTTCAACTGC TTCAGCAAAT 
GGAAACCTTC AGCAATCGCC AGTTTACCAG CAGACTTCTC ACATGATGAG TGCATTGTCT 
ACCAATGAGG ATATGCAAAT GCAGTGTGAA TTGTTTTCTT CTCCTCCTGC AGTTTCTGGA 
AATGAAACTT CTACAACTAC CACACAGCAG GTTGCAACCC CTGGCACTAC CATGTTTCAG 
ACATCAAGTT CAGGAGATGG AGAAGAAACT GGAACACAAG CAAAACAGAT TCAGAACAGT 
GTCTTTCAGA CCATGGTCCA AATGCAACAT AGTGGGGACA ATCAACCTCA AGTTAACCTT 
TTTTCATCCA CAAAAAGTAT GATGAGTGTT CAGAATAGTG GTACCCAACA ACAAGGTAAT 
GGTTTATTCC AGCAAGGGAA TGAGATGATG TCACTTCAAT CTGGAAATTT TTTGCAGCAG 
TCTTCTCATT CACAGGCCCA ACTTTTTCAT CCTCAAAATC CTATTGCCGA TGCTCAGAAC 
CTTTCCCAGG AAACTCAAGG TTCTCTCTTT CATAGTCCAA ATCCTATTGT CCACAGTCAG 
ACTTCTACAA CCTCCTCTGA ACAAATGCAG CCTCCAATGT TTCACTCTCA AAGTACCATT 
GCTGTGTTAC AGGGCTCTTC AGTTCCTCAA GACCAGCAGT CAACCAACAT ATTTCTTTCC 
CAGAGTCCCA TGAATAATCT TCAGACTAAC ACAGTAGCCC AAGAAGCATT TTTTGCAGCA 
CCGAACTCAA TTTCTCCACT TCAGTCAACA TCAAACAGTG AACAACAAGC TGCTTTCCAA 
CAGCAAGCTC CAATATCACA CATCCAGACT CCTATGCTTT CCCAAGAACA GGCACAACCC 
CCGCAGCAGG GTTTATTTCA GCCTCAGGTG GCCCTGGGCT CCCTTCCACC TAATCCAATG 
CCTCAAAGCC AACAAGGAAC CATGTTCCAG TCACAGCACT CAATAGTTGC CATGCAGAGT 
AACTCTCCAT CCCAGGAACA GCAGCAGCAG CAGCAACAGC AGCAGCAACA GCAGCAGCAA 
CAACAACAGA GCATTTTATT CAGTAATCAG AATACCATGG CTACAATGGC GTCTCCAAAG 
CAACCACCAC CAAACATGAT ATTCAACCCA AATCAAAATC CAATGGCTAA TCAGGAGCAA 
CAGAACCAGT CAATTTTTCA CCAACAAAGT AACATGGCCC CAATGAATCA AGAGCAACAG 
CCCATGCAAT TTCAGAGTCA GTCCACAGTT TCCTCACTTC AGAACCCAGG TCCTACCCAG 
TCGGAATCAT CACAGACCCC CTTGTTCCAT AGCTCTCCTC AGATTCAGTT GGTACAAGGG 
TCACCTAGTT CTCAAGAGCA GCAAGTAACT CTCTTCTTAT CTCCAGCATC CATGTCTGCC 
TTGCAGACCA GTATAAATCA ACAAGATATG CAACAGTCTC CTCTTTATTC CCCTCAGAAC 
AACATGCCTG GAATTCAAGG AGCCACATCT TCGCCTCAAC CACAGGCTAC TTTATTTCAC 
AACACAGCAG GAGGCACAAT GAACCAACTG CAGAATTCTC CTGGCTCATC TCAGCAGACA 
TCAGGAATGT TCTTATTTGG CATTCAAAAT AACTGTAGTC AGCTTTTAAC CTCTGGACCA 
GCTACATTGC CTGATCAGTT GATGGCCATA AGTCAGCCAG GCCAACCACA AAACGAGGGC 
CAGCCACCTG TGACAACACT TCTTTCTCAG CAAATGCCAG AGAATTCTCC ACTGGCATCC 
TCTATAAACA CCAACCAGAA CATCGAAAAG ATTGATTTGC TTGTTTCATT GCAAAACCAA 
GGGAACAACT TGACTGGCTC CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106649.1
CDS	337..4983
Translation	MPSDFISLLSADLDLESPKSLYSRDSLKLHPSQNFHRAGLLEESVYDLLPKELQLPPSRETSVASMSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	NM_001113178.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113178.3

ORF Insert Sequence:

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTCTACTCGC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA 
TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA 
ACATCTGTAG CATCAATGAG TCAGACAAGC GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT 
GTTGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC 
TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT 
ATGCAAGTGG AGAGCTGCTC CTCAGCCGTG GGGGTAAGTA ACAGAGGGGT AAGTGAAAAG 
CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG 
TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG 
GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC 
TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA 
AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG 
GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG 
GACAACAAAG GCAACTCAAA AGCGGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA 
GTAAAGAAGA GCCCTATGTT GTGTGGACAA TATCCTGTTA AAAGTGAGGG AAAGGAGCTG 
AAGATAGTTG TACAACCTGA GACACAGCAC CGAGCTCGGT ACCTGACTGA GGGCAGCCGT 
GGCTCAGTGA AAGATAGAAC ACAGCAAGGC TTTCCTACAG TAAAGCTGGA AGGCCATAAT 
GAACCTGTAG TGTTGCAAGT GTTTGTGGGC AACGACTCTG GACGAGTGAA ACCACATGGA 
TTTTATCAGG CCTGCAGAGT AACTGGACGA AATACAACTC CTTGCAAAGA AGTGGACATT 
GAAGGCACTA CTGTTATAGA AGTCGGCCTT GATCCTAGCA ACAACATGAC ACTGGCGGTG 
GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTCG AAGCCAGAAT AGGAATTGCT 
GGTTCCAAGA AGAAAAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CATGAGGAAA 
GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTGTGTAC TCAGCCAGCA 
GGAGTGCCAG AAATCTTAAA GAAAAGCTTG CATAGCTGTT CAGTGAAAGG AGAAGAAGAA 
GTGTTTTTAA TCGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT 
TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAACTATT TCATCAGAAT 
CATCTTATTG TGAAGGTTCC TCCCTATCAT GACCAACATA TAACTTTGCC TGTGTCAGTG 
GGAATATATG TAGTGACAAA TGCTGGAAGA TCTCATGATG TTCAACCATT CACTTACACT 
CCAGACCCAG CAGCTGGTGC TTTGAATGTA AATGTGAAGA AGGAAATATC TAGTCCAGCA 
AGACCTTGCT CTTTTGAAGA GGCCATGAAA GCAATGAAAA CTACTGGATG TAATTTAGAT 
AAGGTAAATA TTATCCCTAA TGCCCTGATG ACTCCACTCA TACCAAGCAG TATGATTAAG 
AGTGAAGATG TTACTCCAAT GGAAGTAACA GCAGAAAAAA GATCTTCCAC TATTTTTAAG 
ACTACAAAGT CTGTTGGATC AACTCAGCAA ACATTAGAAA ACATCTCAAA CATAGCAGGA 
AATGGCTCTT TTTCATCACC ATCATCTTCC CACCTACCTT CTGAAAATGA AAAACAGCAG 
CAGATTCAGC CCAAGGCATA CAACCCAGAG ACCCTGACAA CTATTCAAAC CCAGGACATC 
TCACAGCCTG GTACTTTTCC AGCAGTTTCT GCTTCTAGTC AGCTGCCCAA CAGCGATGCA 
CTATTGCAGC AGGCTACACA GTTTCAGACA AGAGAAACTC AGTCTAGAGA GATATTACAG 
TCAGATGGTA CAGTGGTTAA TTTGTCACAA CTGACTGAGG CATCACAACA ACAGCAGCAG 
TCACCACTAC AAGAACAAGC ACAGACTTTA CAGCAGCAGA TTTCATCAAA TATTTTTCCA 
TCACCAAATA GTGTGAGTCA GCTTCAGAAT ACTATTCAGC AGCTGCAAGC AGGGAGTTTC 
ACAGGCAGTA CTGCTAGTGG CAGCAGTGGA AGTGTTGACT TGGTCCAACA AGTTTTAGAG 
GCACAGCAGC AGTTATCTTC AGTTTTATTT TCTGCTCCAG ATGGTAATGA GAATGTTCAA 
GAGCAGCTTA GTGCAGATAT TTTTCAACAA GTCAGTCAAA TTCAGAGTGG TGTAAGCCCT 
GGAATGTTTT CCTCAACAGA GCCAACAGTC CATACCAGAC CAGATAATTT ATTACCTGGA 
AGAGCTGAAA GTGTTCATCC ACAGTCTGAA AACACGTTAT CTAATCAACA GCAGCAGCAG 
CAGCAGCAAC AGCAAGTGAT GGAATCTTCA GCCGCAATGG TGATGGAGAT GCAACAGAGT 
ATCTGCCAGG CAGCTGCCCA GATTCAGTCA GAGTTATTCC CTTCAACTGC TTCAGCAAAT 
GGAAACCTTC AGCAATCGCC AGTTTACCAG CAGACTTCTC ACATGATGAG TGCATTGTCT 
ACCAATGAGG ATATGCAAAT GCAGTGTGAA TTGTTTTCTT CTCCTCCTGC AGTTTCTGGA 
AATGAAACTT CTACAACTAC CACACAGCAG GTTGCAACCC CTGGCACTAC CATGTTTCAG 
ACATCAAGTT CAGGAGATGG AGAAGAAACT GGAACACAAG CAAAACAGAT TCAGAACAGT 
GTCTTTCAGA CCATGGTCCA AATGCAACAT AGTGGGGACA ATCAACCTCA AGTTAACCTT 
TTTTCATCCA CAAAAAGTAT GATGAGTGTT CAGAATAGTG GTACCCAACA ACAAGGTAAT 
GGTTTATTCC AGCAAGGGAA TGAGATGATG TCACTTCAAT CTGGAAATTT TTTGCAGCAG 
TCTTCTCATT CACAGGCCCA ACTTTTTCAT CCTCAAAATC CTATTGCCGA TGCTCAGAAC 
CTTTCCCAGG AAACTCAAGG TTCTCTCTTT CATAGTCCAA ATCCTATTGT CCACAGTCAG 
ACTTCTACAA CCTCCTCTGA ACAAATGCAG CCTCCAATGT TTCACTCTCA AAGTACCATT 
GCTGTGTTAC AGGGCTCTTC AGTTCCTCAA GACCAGCAGT CAACCAACAT ATTTCTTTCC 
CAGAGTCCCA TGAATAATCT TCAGACTAAC ACAGTAGCCC AAGAAGCATT TTTTGCAGCA 
CCGAACTCAA TTTCTCCACT TCAGTCAACA TCAAACAGTG AACAACAAGC TGCTTTCCAA 
CAGCAAGCTC CAATATCACA CATCCAGACT CCTATGCTTT CCCAAGAACA GGCACAACCC 
CCGCAGCAGG GTTTATTTCA GCCTCAGGTG GCCCTGGGCT CCCTTCCACC TAATCCAATG 
CCTCAAAGCC AACAAGGAAC CATGTTCCAG TCACAGCACT CAATAGTTGC CATGCAGAGT 
AACTCTCCAT CCCAGGAACA GCAGCAGCAG CAGCAACAGC AGCAGCAACA GCAGCAGCAA 
CAACAACAGA GCATTTTATT CAGTAATCAG AATACCATGG CTACAATGGC GTCTCCAAAG 
CAACCACCAC CAAACATGAT ATTCAACCCA AATCAAAATC CAATGGCTAA TCAGGAGCAA 
CAGAACCAGT CAATTTTTCA CCAACAAAGT AACATGGCCC CAATGAATCA AGAGCAACAG 
CCCATGCAAT TTCAGAGTCA GTCCACAGTT TCCTCACTTC AGAACCCAGG TCCTACCCAG 
TCGGAATCAT CACAGACCCC CTTGTTCCAT AGCTCTCCTC AGATTCAGTT GGTACAAGGG 
TCACCTAGTT CTCAAGAGCA GCAAGTAACT CTCTTCTTAT CTCCAGCATC CATGTCTGCC 
TTGCAGACCA GTATAAATCA ACAAGATATG CAACAGTCTC CTCTTTATTC CCCTCAGAAC 
AACATGCCTG GAATTCAAGG AGCCACATCT TCGCCTCAAC CACAGGCTAC TTTATTTCAC 
AACACAGCAG GAGGCACAAT GAACCAACTG CAGAATTCTC CTGGCTCATC TCAGCAGACA 
TCAGGAATGT TCTTATTTGG CATTCAAAAT AACTGTAGTC AGCTTTTAAC CTCTGGACCA 
GCTACATTGC CTGATCAGTT GATGGCCATA AGTCAGCCAG GCCAACCACA AAACGAGGGC 
CAGCCACCTG TGACAACACT TCTTTCTCAG CAAATGCCAG AGAATTCTCC ACTGGCATCC 
TCTATAAACA CCAACCAGAA CATCGAAAAG ATTGATTTGC TTGTTTCATT GCAAAACCAA 
GGGAACAACT TGACTGGCTC CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( NM_173215.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_775322.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu25124
Clone ID Related Accession (Same CDS sequence)	XM_011522818.3 , XM_017022870.2 , NM_173215.3 , XM_017022870.1 , NM_173214.3 , NM_138714.4 , XM_011522818.2 , XM_011522818.1 , NM_173214.2 , NM_138714.3 , NM_173215.2
Accession Version	NM_173215.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009032.8, AJ243298.1, AF163836.1, AJ005683.1, AA321843.1 and AC012321.8. On May 31, 2019 this sequence version replaced NM_173215.2. Transcript Variant: This variant (5) differs in the 5' and 3' UTRs, compared to variant 1. It encodes isoform a, which is also encoded by variants 1 and 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AJ005683.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775322.1
CDS	627..4994
Translation	MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	NM_173215.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173215.3

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( NM_173214.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_775321.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu25124
Clone ID Related Accession (Same CDS sequence)	XM_011522818.3 , XM_017022870.2 , NM_173215.3 , XM_017022870.1 , NM_173214.3 , NM_138714.4 , XM_011522818.2 , XM_011522818.1 , NM_173214.2 , NM_138714.3 , NM_173215.2
Accession Version	NM_173214.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009032.8, AF089824.1, AC012321.8 and AA291524.1. On May 31, 2019 this sequence version replaced NM_173214.2. Transcript Variant: This variant (4) lacks an exon in the 5' UTR, compared to variant 1. Variants 1, 4 and 5 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF089824.1, AJ243298.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775321.1
CDS	627..4994
Translation	MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	NM_173214.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173214.3

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( NM_138714.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_619728.2 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 1, mRNA.

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CloneID	OHu25124
Clone ID Related Accession (Same CDS sequence)	XM_011522818.3 , XM_017022870.2 , NM_173215.3 , XM_017022870.1 , NM_173214.3 , NM_138714.4 , XM_011522818.2 , XM_011522818.1 , NM_173214.2 , NM_138714.3 , NM_173215.2
Accession Version	NM_138714.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF346509.1, AB020634.1, AC012321.8 and AA291524.1. On May 31, 2019 this sequence version replaced NM_138714.3. Transcript Variant: This variant (1) represents the longest transcript. This variant and variants 4 and 5 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF346509.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619728.2
CDS	681..5048
Translation	MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	NM_138714.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138714.4

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( NM_173214.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_775321.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 4, mRNA.

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CloneID	OHu25124
Clone ID Related Accession (Same CDS sequence)	XM_011522818.3 , XM_017022870.2 , NM_173215.3 , XM_017022870.1 , NM_173214.3 , NM_138714.4 , XM_011522818.2 , XM_011522818.1 , NM_173214.2 , NM_138714.3 , NM_173215.2
Accession Version	NM_173214.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009032.8, AF089824.1, AC012321.8 and AA291524.1. On Aug 4, 2011 this sequence version replaced NM_173214.1. Transcript Variant: This variant (4) lacks an exon in the 5' UTR, compared to variant 1. Variants 1, 4 and 5 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF089824.1, AJ243298.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775321.1
CDS	627..4994
Translation	MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	NM_173214.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173214.2

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( NM_138714.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_619728.2 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25124
Clone ID Related Accession (Same CDS sequence)	XM_011522818.3 , XM_017022870.2 , NM_173215.3 , XM_017022870.1 , NM_173214.3 , NM_138714.4 , XM_011522818.2 , XM_011522818.1 , NM_173214.2 , NM_138714.3 , NM_173215.2
Accession Version	NM_138714.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF346509.1, AB020634.1, AC012321.8 and AA291524.1. On Aug 4, 2011 this sequence version replaced NM_138714.2. Transcript Variant: This variant (1) represents the longest transcript. This variant and variants 4 and 5 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF346509.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619728.2
CDS	681..5048
Translation	MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	NM_138714.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138714.3

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( NM_173215.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_775322.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu25124
Clone ID Related Accession (Same CDS sequence)	XM_011522818.3 , XM_017022870.2 , NM_173215.3 , XM_017022870.1 , NM_173214.3 , NM_138714.4 , XM_011522818.2 , XM_011522818.1 , NM_173214.2 , NM_138714.3 , NM_173215.2
Accession Version	NM_173215.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009032.8, AJ243298.1, AF163836.1, AJ005683.1 and AA321843.1. On Aug 4, 2011 this sequence version replaced NM_173215.1. Transcript Variant: This variant (5) differs in the 5' and 3' UTRs, compared to variant 1. It encodes isoform a, which is also encoded by variants 1 and 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF089824.1, AF134870.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775322.1
CDS	627..4994
Translation	MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	NM_173215.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173215.2

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_011522818.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521120.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X3, mRNA.

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CloneID	OHu25124
Clone ID Related Accession (Same CDS sequence)	XM_011522818.3 , XM_017022870.2 , NM_173215.3 , XM_017022870.1 , NM_173214.3 , NM_138714.4 , XM_011522818.2 , XM_011522818.1 , NM_173214.2 , NM_138714.3 , NM_173215.2
Accession Version	XM_011522818.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011522818.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521120.1
CDS	754..5121
Translation	MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_011522818.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522818.3

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_017022870.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878359.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X4, mRNA.

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CloneID	OHu25124
Clone ID Related Accession (Same CDS sequence)	XM_011522818.3 , XM_017022870.2 , NM_173215.3 , XM_017022870.1 , NM_173214.3 , NM_138714.4 , XM_011522818.2 , XM_011522818.1 , NM_173214.2 , NM_138714.3 , NM_173215.2
Accession Version	XM_017022870.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878359.1
CDS	914..5281
Translation	MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_017022870.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022870.2

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_017022870.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878359.1 Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X4, mRNA.

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CloneID	OHu25124
Clone ID Related Accession (Same CDS sequence)	XM_011522818.3 , XM_017022870.2 , NM_173215.3 , XM_017022870.1 , NM_173214.3 , NM_138714.4 , XM_011522818.2 , XM_011522818.1 , NM_173214.2 , NM_138714.3 , NM_173215.2
Accession Version	XM_017022870.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878359.1
CDS	914..5281
Translation	MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_017022870.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022870.1

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_011522818.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521120.1 Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X3, mRNA.

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CloneID	OHu25124
Clone ID Related Accession (Same CDS sequence)	XM_011522818.3 , XM_017022870.2 , NM_173215.3 , XM_017022870.1 , NM_173214.3 , NM_138714.4 , XM_011522818.2 , XM_011522818.1 , NM_173214.2 , NM_138714.3 , NM_173215.2
Accession Version	XM_011522818.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522818.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521120.1
CDS	754..5121
Translation	MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_011522818.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522818.2

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_011522818.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521120.1 Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X3, mRNA.

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CloneID	OHu25124
Clone ID Related Accession (Same CDS sequence)	XM_011522818.3 , XM_017022870.2 , NM_173215.3 , XM_017022870.1 , NM_173214.3 , NM_138714.4 , XM_011522818.2 , XM_011522818.1 , NM_173214.2 , NM_138714.3 , NM_173215.2
Accession Version	XM_011522818.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	nuclear factor of activated T-cells 5 isoform X1
Comment

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011521120.1

Translation

MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_011522818.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522818.1

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAGGAAATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGAT GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC 
ATACCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCA CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA 
AACATCTCAA ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT 
TCTGAAAATG AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA 
ACTATTCAAA CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT 
CAGCTGCCCA ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT 
CAGTCTAGAG AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG 
GCATCACAAC AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG 
ATTTCATCAA ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG 
CAGCTGCAAG CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA 
ATTCAGAGTG GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA 
CCAGATAATT TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA 
TCTAATCAAC AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG 
GTGATGGAGA TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC 
CCTTCAACTG CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT 
CACATGATGA GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT 
TCTCCTCCTG CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC 
CCTGGCACTA CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA 
GCAAAACAGA TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC 
AATCAACCTC AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT 
GGTACCCAAC AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA 
TCTGGAAATT TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT 
CCTATTGCCG ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA 
AATCCTATTG TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG 
TTTCACTCTC AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG 
TCAACCAACA TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC 
CAAGAAGCAT TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT 
GAACAACAAG CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT 
TCCCAAGAAC AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCAC CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC 
TCAATAGTTG CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAC AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG 
GCTACAATGG CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT 
CCAATGGCTA ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC 
CCAATGAATC AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT 
CAGAACCCAG GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT 
CAGATTCAGT TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA 
TCTCCAGCAT CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT 
CCTCTTTATT CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA 
CCACAGGCTA CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT 
CCTGGCTCAT CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT 
CAGCTTTTAA CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA 
GGCCAACCAC AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAGAATTCTC CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG 
CTTGTTTCAT TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( NM_138713.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_619727.2 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 2, mRNA.

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CloneID	OHu10608
Clone ID Related Accession (Same CDS sequence)	NM_138713.4 , NM_138713.3
Accession Version	NM_138713.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009032.8, AJ243299.1, BC131509.1, AC012321.8 and AA291524.1. On Aug 4, 2011 this sequence version replaced NM_138713.2. Transcript Variant: This variant (2) lacks an exon in the 5' region, compared to variant 1. This difference causes translation initiation at an upstream start codon and results in an isoform (b) with a longer N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ243299.1, SRR1803615.141797.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTCTACTCGC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA 
TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA 
ACATCTGTAG CATCAATGAG TCAGACAAGC GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT 
GTTGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC 
TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT 
ATGCAAGTGG AGAGCTGCTC CTCAGCCGTG GGGGTAAGTA ACAGAGGGGT AAGTGAAAAG 
CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG 
TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG 
GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC 
TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA 
AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG 
GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG 
GACAACAAAG GCAACTCAAA AGCGGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA 
GTAAAGAAGA GCCCTATGTT GTGTGGACAA TATCCTGTTA AAAGTGAGGG AAAGGAGCTG 
AAGATAGTTG TACAACCTGA GACACAGCAC CGAGCTCGGT ACCTGACTGA GGGCAGCCGT 
GGCTCAGTGA AAGATAGAAC ACAGCAAGGC TTTCCTACAG TAAAGCTGGA AGGCCATAAT 
GAACCTGTAG TGTTGCAAGT GTTTGTGGGC AACGACTCTG GACGAGTGAA ACCACATGGA 
TTTTATCAGG CCTGCAGAGT AACTGGACGA AATACAACTC CTTGCAAAGA AGTGGACATT 
GAAGGCACTA CTGTTATAGA AGTCGGCCTT GATCCTAGCA ACAACATGAC ACTGGCGGTG 
GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTCG AAGCCAGAAT AGGAATTGCT 
GGTTCCAAGA AGAAAAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CATGAGGAAA 
GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTGTGTAC TCAGCCAGCA 
GGAGTGCCAG AAATCTTAAA GAAAAGCTTG CATAGCTGTT CAGTGAAAGG AGAAGAAGAA 
GTGTTTTTAA TCGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT 
TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAACTATT TCATCAGAAT 
CATCTTATTG TGAAGGTTCC TCCCTATCAT GACCAACATA TAACTTTGCC TGTGTCAGTG 
GGAATATATG TAGTGACAAA TGCTGGAAGA TCTCATGATG TTCAACCATT CACTTACACT 
CCAGACCCAG CAGCAGCTGG TGCTTTGAAT GTAAATGTGA AGAAGGAAAT ATCTAGTCCA 
GCAAGACCTT GCTCTTTTGA AGAGGCCATG AAAGCAATGA AAACTACTGG ATGTAATTTA 
GATAAGGTAA ATATTATCCC TAATGCCCTG ATGACTCCAC TCATACCAAG CAGTATGATT 
AAGAGTGAAG ATGTTACTCC AATGGAAGTA ACAGCAGAAA AAAGATCTTC CACTATTTTT 
AAGACTACAA AGTCTGTTGG ATCAACTCAG CAAACATTAG AAAACATCTC AAACATAGCA 
GGAAATGGCT CTTTTTCATC ACCATCATCT TCCCACCTAC CTTCTGAAAA TGAAAAACAG 
CAGCAGATTC AGCCCAAGGC ATACAACCCA GAGACCCTGA CAACTATTCA AACCCAGGAC 
ATCTCACAGC CTGGTACTTT TCCAGCAGTT TCTGCTTCTA GTCAGCTGCC CAACAGCGAT 
GCACTATTGC AGCAGGCTAC ACAGTTTCAG ACAAGAGAAA CTCAGTCTAG AGAGATATTA 
CAGTCAGATG GTACAGTGGT TAATTTGTCA CAACTGACTG AGGCATCACA ACAACAGCAG 
CAGTCACCAC TACAAGAACA AGCACAGACT TTACAGCAGC AGATTTCATC AAATATTTTT 
CCATCACCAA ATAGTGTGAG TCAGCTTCAG AATACTATTC AGCAGCTGCA AGCAGGGAGT 
TTCACAGGCA GTACTGCTAG TGGCAGCAGT GGAAGTGTTG ACTTGGTCCA ACAAGTTTTA 
GAGGCACAGC AGCAGTTATC TTCAGTTTTA TTTTCTGCTC CAGATGGTAA TGAGAATGTT 
CAAGAGCAGC TTAGTGCAGA TATTTTTCAA CAAGTCAGTC AAATTCAGAG TGGTGTAAGC 
CCTGGAATGT TTTCCTCAAC AGAGCCAACA GTCCATACCA GACCAGATAA TTTATTACCT 
GGAAGAGCTG AAAGTGTTCA TCCACAGTCT GAAAACACGT TATCTAATCA ACAGCAGCAG 
CAGCAGCAGC AACAGCAAGT GATGGAATCT TCAGCCGCAA TGGTGATGGA GATGCAACAG 
AGTATCTGCC AGGCAGCTGC CCAGATTCAG TCAGAGTTAT TCCCTTCAAC TGCTTCAGCA 
AATGGAAACC TTCAGCAATC GCCAGTTTAC CAGCAGACTT CTCACATGAT GAGTGCATTG 
TCTACCAATG AGGATATGCA AATGCAGTGT GAATTGTTTT CTTCTCCTCC TGCAGTTTCT 
GGAAATGAAA CTTCTACAAC TACCACACAG CAGGTTGCAA CCCCTGGCAC TACCATGTTT 
CAGACATCAA GTTCAGGAGA TGGAGAAGAA ACTGGAACAC AAGCAAAACA GATTCAGAAC 
AGTGTCTTTC AGACCATGGT CCAAATGCAA CATAGTGGGG ACAATCAACC TCAAGTTAAC 
CTTTTTTCAT CCACAAAAAG TATGATGAGT GTTCAGAATA GTGGTACCCA ACAACAAGGT 
AATGGTTTAT TCCAGCAAGG GAATGAGATG ATGTCACTTC AATCTGGAAA TTTTTTGCAG 
CAGTCTTCTC ATTCACAGGC CCAACTTTTT CATCCTCAAA ATCCTATTGC CGATGCTCAG 
AACCTTTCCC AGGAAACTCA AGGTTCTCTC TTTCATAGTC CAAATCCTAT TGTCCACAGT 
CAGACTTCTA CAACCTCCTC TGAACAAATG CAGCCTCCAA TGTTTCACTC TCAAAGTACC 
ATTGCTGTGT TACAGGGCTC TTCAGTTCCT CAAGACCAGC AGTCAACCAA CATATTTCTT 
TCCCAGAGTC CCATGAATAA TCTTCAGACT AACACAGTAG CCCAAGAAGC ATTTTTTGCA 
GCACCGAACT CAATTTCTCC ACTTCAGTCA ACATCAAACA GTGAACAACA AGCTGCTTTC 
CAACAGCAAG CTCCAATATC ACACATCCAG ACTCCTATGC TTTCCCAAGA ACAGGCACAA 
CCCCCGCAGC AGGGTTTATT TCAGCCTCAG GTGGCCCTGG GCTCCCTTCC ACCTAATCCA 
ATGCCTCAAA GCCAACAAGG AACCATGTTC CAGTCACAGC ACTCAATAGT TGCCATGCAG 
AGTAACTCTC CATCCCAGGA ACAGCAGCAG CAGCAGCAAC AGCAGCAGCA ACAGCAGCAG 
CAACAACAAC AGAGCATTTT ATTCAGTAAT CAGAATACCA TGGCTACAAT GGCGTCTCCA 
AAGCAACCAC CACCAAACAT GATATTCAAC CCAAATCAAA ATCCAATGGC TAATCAGGAG 
CAACAGAACC AGTCAATTTT TCACCAACAA AGTAACATGG CCCCAATGAA TCAAGAGCAA 
CAGCCCATGC AATTTCAGAG TCAGTCCACA GTTTCCTCAC TTCAGAACCC AGGTCCTACC 
CAGTCGGAAT CATCACAGAC CCCCTTGTTC CATAGCTCTC CTCAGATTCA GTTGGTACAA 
GGGTCACCTA GTTCTCAAGA GCAGCAAGTA ACTCTCTTCT TATCTCCAGC ATCCATGTCT 
GCCTTGCAGA CCAGTATAAA TCAACAAGAT ATGCAACAGT CTCCTCTTTA TTCCCCTCAG 
AACAACATGC CTGGAATTCA AGGAGCCACA TCTTCGCCTC AACCACAGGC TACTTTATTT 
CACAACACAG CAGGAGGCAC AATGAACCAA CTGCAGAATT CTCCTGGCTC ATCTCAGCAG 
ACATCAGGAA TGTTCTTATT TGGCATTCAA AATAACTGTA GTCAGCTTTT AACCTCTGGA 
CCAGCTACAT TGCCTGATCA GTTGATGGCC ATAAGTCAGC CAGGCCAACC ACAAAACGAG 
GGCCAGCCAC CTGTGACAAC ACTTCTTTCT CAGCAAATGC CAGAGAATTC TCCACTGGCA 
TCCTCTATAA ACACCAACCA GAACATCGAA AAGATTGATT TGCTTGTTTC ATTGCAAAAC 
CAAGGGAACA ACTTGACTGG CTCCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619727.2
CDS	337..4986
Translation	MPSDFISLLSADLDLESPKSLYSRDSLKLHPSQNFHRAGLLEESVYDLLPKELQLPPSRETSVASMSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	NM_138713.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138713.3

ORF Insert Sequence:

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTCTACTCGC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA 
TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA 
ACATCTGTAG CATCAATGAG TCAGACAAGC GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT 
GTTGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC 
TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT 
ATGCAAGTGG AGAGCTGCTC CTCAGCCGTG GGGGTAAGTA ACAGAGGGGT AAGTGAAAAG 
CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG 
TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG 
GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC 
TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA 
AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG 
GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG 
GACAACAAAG GCAACTCAAA AGCGGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA 
GTAAAGAAGA GCCCTATGTT GTGTGGACAA TATCCTGTTA AAAGTGAGGG AAAGGAGCTG 
AAGATAGTTG TACAACCTGA GACACAGCAC CGAGCTCGGT ACCTGACTGA GGGCAGCCGT 
GGCTCAGTGA AAGATAGAAC ACAGCAAGGC TTTCCTACAG TAAAGCTGGA AGGCCATAAT 
GAACCTGTAG TGTTGCAAGT GTTTGTGGGC AACGACTCTG GACGAGTGAA ACCACATGGA 
TTTTATCAGG CCTGCAGAGT AACTGGACGA AATACAACTC CTTGCAAAGA AGTGGACATT 
GAAGGCACTA CTGTTATAGA AGTCGGCCTT GATCCTAGCA ACAACATGAC ACTGGCGGTG 
GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTCG AAGCCAGAAT AGGAATTGCT 
GGTTCCAAGA AGAAAAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CATGAGGAAA 
GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTGTGTAC TCAGCCAGCA 
GGAGTGCCAG AAATCTTAAA GAAAAGCTTG CATAGCTGTT CAGTGAAAGG AGAAGAAGAA 
GTGTTTTTAA TCGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT 
TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAACTATT TCATCAGAAT 
CATCTTATTG TGAAGGTTCC TCCCTATCAT GACCAACATA TAACTTTGCC TGTGTCAGTG 
GGAATATATG TAGTGACAAA TGCTGGAAGA TCTCATGATG TTCAACCATT CACTTACACT 
CCAGACCCAG CAGCAGCTGG TGCTTTGAAT GTAAATGTGA AGAAGGAAAT ATCTAGTCCA 
GCAAGACCTT GCTCTTTTGA AGAGGCCATG AAAGCAATGA AAACTACTGG ATGTAATTTA 
GATAAGGTAA ATATTATCCC TAATGCCCTG ATGACTCCAC TCATACCAAG CAGTATGATT 
AAGAGTGAAG ATGTTACTCC AATGGAAGTA ACAGCAGAAA AAAGATCTTC CACTATTTTT 
AAGACTACAA AGTCTGTTGG ATCAACTCAG CAAACATTAG AAAACATCTC AAACATAGCA 
GGAAATGGCT CTTTTTCATC ACCATCATCT TCCCACCTAC CTTCTGAAAA TGAAAAACAG 
CAGCAGATTC AGCCCAAGGC ATACAACCCA GAGACCCTGA CAACTATTCA AACCCAGGAC 
ATCTCACAGC CTGGTACTTT TCCAGCAGTT TCTGCTTCTA GTCAGCTGCC CAACAGCGAT 
GCACTATTGC AGCAGGCTAC ACAGTTTCAG ACAAGAGAAA CTCAGTCTAG AGAGATATTA 
CAGTCAGATG GTACAGTGGT TAATTTGTCA CAACTGACTG AGGCATCACA ACAACAGCAG 
CAGTCACCAC TACAAGAACA AGCACAGACT TTACAGCAGC AGATTTCATC AAATATTTTT 
CCATCACCAA ATAGTGTGAG TCAGCTTCAG AATACTATTC AGCAGCTGCA AGCAGGGAGT 
TTCACAGGCA GTACTGCTAG TGGCAGCAGT GGAAGTGTTG ACTTGGTCCA ACAAGTTTTA 
GAGGCACAGC AGCAGTTATC TTCAGTTTTA TTTTCTGCTC CAGATGGTAA TGAGAATGTT 
CAAGAGCAGC TTAGTGCAGA TATTTTTCAA CAAGTCAGTC AAATTCAGAG TGGTGTAAGC 
CCTGGAATGT TTTCCTCAAC AGAGCCAACA GTCCATACCA GACCAGATAA TTTATTACCT 
GGAAGAGCTG AAAGTGTTCA TCCACAGTCT GAAAACACGT TATCTAATCA ACAGCAGCAG 
CAGCAGCAGC AACAGCAAGT GATGGAATCT TCAGCCGCAA TGGTGATGGA GATGCAACAG 
AGTATCTGCC AGGCAGCTGC CCAGATTCAG TCAGAGTTAT TCCCTTCAAC TGCTTCAGCA 
AATGGAAACC TTCAGCAATC GCCAGTTTAC CAGCAGACTT CTCACATGAT GAGTGCATTG 
TCTACCAATG AGGATATGCA AATGCAGTGT GAATTGTTTT CTTCTCCTCC TGCAGTTTCT 
GGAAATGAAA CTTCTACAAC TACCACACAG CAGGTTGCAA CCCCTGGCAC TACCATGTTT 
CAGACATCAA GTTCAGGAGA TGGAGAAGAA ACTGGAACAC AAGCAAAACA GATTCAGAAC 
AGTGTCTTTC AGACCATGGT CCAAATGCAA CATAGTGGGG ACAATCAACC TCAAGTTAAC 
CTTTTTTCAT CCACAAAAAG TATGATGAGT GTTCAGAATA GTGGTACCCA ACAACAAGGT 
AATGGTTTAT TCCAGCAAGG GAATGAGATG ATGTCACTTC AATCTGGAAA TTTTTTGCAG 
CAGTCTTCTC ATTCACAGGC CCAACTTTTT CATCCTCAAA ATCCTATTGC CGATGCTCAG 
AACCTTTCCC AGGAAACTCA AGGTTCTCTC TTTCATAGTC CAAATCCTAT TGTCCACAGT 
CAGACTTCTA CAACCTCCTC TGAACAAATG CAGCCTCCAA TGTTTCACTC TCAAAGTACC 
ATTGCTGTGT TACAGGGCTC TTCAGTTCCT CAAGACCAGC AGTCAACCAA CATATTTCTT 
TCCCAGAGTC CCATGAATAA TCTTCAGACT AACACAGTAG CCCAAGAAGC ATTTTTTGCA 
GCACCGAACT CAATTTCTCC ACTTCAGTCA ACATCAAACA GTGAACAACA AGCTGCTTTC 
CAACAGCAAG CTCCAATATC ACACATCCAG ACTCCTATGC TTTCCCAAGA ACAGGCACAA 
CCCCCGCAGC AGGGTTTATT TCAGCCTCAG GTGGCCCTGG GCTCCCTTCC ACCTAATCCA 
ATGCCTCAAA GCCAACAAGG AACCATGTTC CAGTCACAGC ACTCAATAGT TGCCATGCAG 
AGTAACTCTC CATCCCAGGA ACAGCAGCAG CAGCAGCAAC AGCAGCAGCA ACAGCAGCAG 
CAACAACAAC AGAGCATTTT ATTCAGTAAT CAGAATACCA TGGCTACAAT GGCGTCTCCA 
AAGCAACCAC CACCAAACAT GATATTCAAC CCAAATCAAA ATCCAATGGC TAATCAGGAG 
CAACAGAACC AGTCAATTTT TCACCAACAA AGTAACATGG CCCCAATGAA TCAAGAGCAA 
CAGCCCATGC AATTTCAGAG TCAGTCCACA GTTTCCTCAC TTCAGAACCC AGGTCCTACC 
CAGTCGGAAT CATCACAGAC CCCCTTGTTC CATAGCTCTC CTCAGATTCA GTTGGTACAA 
GGGTCACCTA GTTCTCAAGA GCAGCAAGTA ACTCTCTTCT TATCTCCAGC ATCCATGTCT 
GCCTTGCAGA CCAGTATAAA TCAACAAGAT ATGCAACAGT CTCCTCTTTA TTCCCCTCAG 
AACAACATGC CTGGAATTCA AGGAGCCACA TCTTCGCCTC AACCACAGGC TACTTTATTT 
CACAACACAG CAGGAGGCAC AATGAACCAA CTGCAGAATT CTCCTGGCTC ATCTCAGCAG 
ACATCAGGAA TGTTCTTATT TGGCATTCAA AATAACTGTA GTCAGCTTTT AACCTCTGGA 
CCAGCTACAT TGCCTGATCA GTTGATGGCC ATAAGTCAGC CAGGCCAACC ACAAAACGAG 
GGCCAGCCAC CTGTGACAAC ACTTCTTTCT CAGCAAATGC CAGAGAATTC TCCACTGGCA 
TCCTCTATAA ACACCAACCA GAACATCGAA AAGATTGATT TGCTTGTTTC ATTGCAAAAC 
CAAGGGAACA ACTTGACTGG CTCCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( NM_006599.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006590.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 3, mRNA.

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CloneID	OHu11182
Clone ID Related Accession (Same CDS sequence)	NM_006599.4 , NM_006599.3
Accession Version	NM_006599.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009032.8, AB020634.1, AC012321.8 and AA291524.1. On May 31, 2019 this sequence version replaced NM_006599.3. Transcript Variant: This variant (3) lacks two exons in the 5' region, compared to variant 1. This difference causes translation initiation at an upstream start codon and results in an isoform (c) with a longer N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.164252.1, BC146765.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTCTACTCGC GAGAATCTGT CTATGATCTT CTCCCAAAGG AGTTACAGTT ACCTCCATCT 
AGAGAAACAT CTGTAGCATC AATGAGTCAG ACAAGCGGTG GTGAGGCAGG CTCGCCTCCT 
CCAGCTGTTG TTGCTGCTGA TGCTTCTTCA GCTCCCTCCT CTTCCTCCAT GGGCGGTGCT 
TGCAGCTCCT TTACCACCTC TTCCAGCCCT ACCATTTATT CTACCTCAGT CACCGACAGC 
AAGGCTATGC AAGTGGAGAG CTGCTCCTCA GCCGTGGGGG TAAGTAACAG AGGGGTAAGT 
GAAAAGCAGT TAACCAGTAA CACAGTTCAG CAGCATCCAT CAACACCGAA GAGGCACACA 
GTCTTGTACA TCTCACCACC ACCTGAGGAC TTGCTGGATA ACAGTCGGAT GTCCTGCCAG 
GATGAGGGGT GTGGATTGGA ATCTGAGCAG AGCTGCAGTA TGTGGATGGA GGATTCCCCC 
TCCAACTTCA GTAACATGAG CACCAGTTCC TACAATGATA ACACTGAGGT ACCTCGTAAA 
TCACGAAAAC GAAATCCAAA GCAGAGGCCG GGGGTCAAAC GACGAGATTG TGAAGAATCT 
AATATGGATA TATTTGATGC CGACAGTGCC AAAGCACCTC ACTATGTGCT TTCTCAGCTT 
ACCACGGACA ACAAAGGCAA CTCAAAAGCG GGAAATGGAA CATTGGAAAA CCAAAAAGGA 
ACTGGAGTAA AGAAGAGCCC TATGTTGTGT GGACAATATC CTGTTAAAAG TGAGGGAAAG 
GAGCTGAAGA TAGTTGTACA ACCTGAGACA CAGCACCGAG CTCGGTACCT GACTGAGGGC 
AGCCGTGGCT CAGTGAAAGA TAGAACACAG CAAGGCTTTC CTACAGTAAA GCTGGAAGGC 
CATAATGAAC CTGTAGTGTT GCAAGTGTTT GTGGGCAACG ACTCTGGACG AGTGAAACCA 
CATGGATTTT ATCAGGCCTG CAGAGTAACT GGACGAAATA CAACTCCTTG CAAAGAAGTG 
GACATTGAAG GCACTACTGT TATAGAAGTC GGCCTTGATC CTAGCAACAA CATGACACTG 
GCGGTGGACT GCGTAGGGAT ATTGAAATTG AGGAATGCTG ATGTCGAAGC CAGAATAGGA 
ATTGCTGGTT CCAAGAAGAA AAGCACTCGT GCCAGATTGG TTTTTCGAGT TAATATCATG 
AGGAAAGATG GCTCCACTTT GACACTGCAA ACACCCTCTT CTCCAATTTT GTGTACTCAG 
CCAGCAGGAG TGCCAGAAAT CTTAAAGAAA AGCTTGCATA GCTGTTCAGT GAAAGGAGAA 
GAAGAAGTGT TTTTAATCGG CAAGAACTTT CTGAAAGGAA CTAAAGTTAT TTTCCAAGAA 
AATGTTTCTG ATGAAAACTC TTGGAAGTCA GAAGCTGAAA TTGATATGGA ACTATTTCAT 
CAGAATCATC TTATTGTGAA GGTTCCTCCC TATCATGACC AACATATAAC TTTGCCTGTG 
TCAGTGGGAA TATATGTAGT GACAAATGCT GGAAGATCTC ATGATGTTCA ACCATTCACT 
TACACTCCAG ACCCAGCAGC AGCTGGTGCT TTGAATGTAA ATGTGAAGAA GGAAATATCT 
AGTCCAGCAA GACCTTGCTC TTTTGAAGAG GCCATGAAAG CAATGAAAAC TACTGGATGT 
AATTTAGATA AGGTAAATAT TATCCCTAAT GCCCTGATGA CTCCACTCAT ACCAAGCAGT 
ATGATTAAGA GTGAAGATGT TACTCCAATG GAAGTAACAG CAGAAAAAAG ATCTTCCACT 
ATTTTTAAGA CTACAAAGTC TGTTGGATCA ACTCAGCAAA CATTAGAAAA CATCTCAAAC 
ATAGCAGGAA ATGGCTCTTT TTCATCACCA TCATCTTCCC ACCTACCTTC TGAAAATGAA 
AAACAGCAGC AGATTCAGCC CAAGGCATAC AACCCAGAGA CCCTGACAAC TATTCAAACC 
CAGGACATCT CACAGCCTGG TACTTTTCCA GCAGTTTCTG CTTCTAGTCA GCTGCCCAAC 
AGCGATGCAC TATTGCAGCA GGCTACACAG TTTCAGACAA GAGAAACTCA GTCTAGAGAG 
ATATTACAGT CAGATGGTAC AGTGGTTAAT TTGTCACAAC TGACTGAGGC ATCACAACAA 
CAGCAGCAGT CACCACTACA AGAACAAGCA CAGACTTTAC AGCAGCAGAT TTCATCAAAT 
ATTTTTCCAT CACCAAATAG TGTGAGTCAG CTTCAGAATA CTATTCAGCA GCTGCAAGCA 
GGGAGTTTCA CAGGCAGTAC TGCTAGTGGC AGCAGTGGAA GTGTTGACTT GGTCCAACAA 
GTTTTAGAGG CACAGCAGCA GTTATCTTCA GTTTTATTTT CTGCTCCAGA TGGTAATGAG 
AATGTTCAAG AGCAGCTTAG TGCAGATATT TTTCAACAAG TCAGTCAAAT TCAGAGTGGT 
GTAAGCCCTG GAATGTTTTC CTCAACAGAG CCAACAGTCC ATACCAGACC AGATAATTTA 
TTACCTGGAA GAGCTGAAAG TGTTCATCCA CAGTCTGAAA ACACGTTATC TAATCAACAG 
CAGCAGCAGC AGCAGCAACA GCAAGTGATG GAATCTTCAG CCGCAATGGT GATGGAGATG 
CAACAGAGTA TCTGCCAGGC AGCTGCCCAG ATTCAGTCAG AGTTATTCCC TTCAACTGCT 
TCAGCAAATG GAAACCTTCA GCAATCGCCA GTTTACCAGC AGACTTCTCA CATGATGAGT 
GCATTGTCTA CCAATGAGGA TATGCAAATG CAGTGTGAAT TGTTTTCTTC TCCTCCTGCA 
GTTTCTGGAA ATGAAACTTC TACAACTACC ACACAGCAGG TTGCAACCCC TGGCACTACC 
ATGTTTCAGA CATCAAGTTC AGGAGATGGA GAAGAAACTG GAACACAAGC AAAACAGATT 
CAGAACAGTG TCTTTCAGAC CATGGTCCAA ATGCAACATA GTGGGGACAA TCAACCTCAA 
GTTAACCTTT TTTCATCCAC AAAAAGTATG ATGAGTGTTC AGAATAGTGG TACCCAACAA 
CAAGGTAATG GTTTATTCCA GCAAGGGAAT GAGATGATGT CACTTCAATC TGGAAATTTT 
TTGCAGCAGT CTTCTCATTC ACAGGCCCAA CTTTTTCATC CTCAAAATCC TATTGCCGAT 
GCTCAGAACC TTTCCCAGGA AACTCAAGGT TCTCTCTTTC ATAGTCCAAA TCCTATTGTC 
CACAGTCAGA CTTCTACAAC CTCCTCTGAA CAAATGCAGC CTCCAATGTT TCACTCTCAA 
AGTACCATTG CTGTGTTACA GGGCTCTTCA GTTCCTCAAG ACCAGCAGTC AACCAACATA 
TTTCTTTCCC AGAGTCCCAT GAATAATCTT CAGACTAACA CAGTAGCCCA AGAAGCATTT 
TTTGCAGCAC CGAACTCAAT TTCTCCACTT CAGTCAACAT CAAACAGTGA ACAACAAGCT 
GCTTTCCAAC AGCAAGCTCC AATATCACAC ATCCAGACTC CTATGCTTTC CCAAGAACAG 
GCACAACCCC CGCAGCAGGG TTTATTTCAG CCTCAGGTGG CCCTGGGCTC CCTTCCACCT 
AATCCAATGC CTCAAAGCCA ACAAGGAACC ATGTTCCAGT CACAGCACTC AATAGTTGCC 
ATGCAGAGTA ACTCTCCATC CCAGGAACAG CAGCAGCAGC AGCAACAGCA GCAGCAACAG 
CAGCAGCAAC AACAACAGAG CATTTTATTC AGTAATCAGA ATACCATGGC TACAATGGCG 
TCTCCAAAGC AACCACCACC AAACATGATA TTCAACCCAA ATCAAAATCC AATGGCTAAT 
CAGGAGCAAC AGAACCAGTC AATTTTTCAC CAACAAAGTA ACATGGCCCC AATGAATCAA 
GAGCAACAGC CCATGCAATT TCAGAGTCAG TCCACAGTTT CCTCACTTCA GAACCCAGGT 
CCTACCCAGT CGGAATCATC ACAGACCCCC TTGTTCCATA GCTCTCCTCA GATTCAGTTG 
GTACAAGGGT CACCTAGTTC TCAAGAGCAG CAAGTAACTC TCTTCTTATC TCCAGCATCC 
ATGTCTGCCT TGCAGACCAG TATAAATCAA CAAGATATGC AACAGTCTCC TCTTTATTCC 
CCTCAGAACA ACATGCCTGG AATTCAAGGA GCCACATCTT CGCCTCAACC ACAGGCTACT 
TTATTTCACA ACACAGCAGG AGGCACAATG AACCAACTGC AGAATTCTCC TGGCTCATCT 
CAGCAGACAT CAGGAATGTT CTTATTTGGC ATTCAAAATA ACTGTAGTCA GCTTTTAACC 
TCTGGACCAG CTACATTGCC TGATCAGTTG ATGGCCATAA GTCAGCCAGG CCAACCACAA 
AACGAGGGCC AGCCACCTGT GACAACACTT CTTTCTCAGC AAATGCCAGA GAATTCTCCA 
CTGGCATCCT CTATAAACAC CAACCAGAAC ATCGAAAAGA TTGATTTGCT TGTTTCATTG 
CAAAACCAAG GGAACAACTT GACTGGCTCC TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006590.1
CDS	337..4932
Translation	MPSDFISLLSADLDLESPKSLYSRESVYDLLPKELQLPPSRETSVASMSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	NM_006599.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006599.4

ORF Insert Sequence:

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTCTACTCGC GAGAATCTGT CTATGATCTT CTCCCAAAGG AGTTACAGTT ACCTCCATCT 
AGAGAAACAT CTGTAGCATC AATGAGTCAG ACAAGCGGTG GTGAGGCAGG CTCGCCTCCT 
CCAGCTGTTG TTGCTGCTGA TGCTTCTTCA GCTCCCTCCT CTTCCTCCAT GGGCGGTGCT 
TGCAGCTCCT TTACCACCTC TTCCAGCCCT ACCATTTATT CTACCTCAGT CACCGACAGC 
AAGGCTATGC AAGTGGAGAG CTGCTCCTCA GCCGTGGGGG TAAGTAACAG AGGGGTAAGT 
GAAAAGCAGT TAACCAGTAA CACAGTTCAG CAGCATCCAT CAACACCGAA GAGGCACACA 
GTCTTGTACA TCTCACCACC ACCTGAGGAC TTGCTGGATA ACAGTCGGAT GTCCTGCCAG 
GATGAGGGGT GTGGATTGGA ATCTGAGCAG AGCTGCAGTA TGTGGATGGA GGATTCCCCC 
TCCAACTTCA GTAACATGAG CACCAGTTCC TACAATGATA ACACTGAGGT ACCTCGTAAA 
TCACGAAAAC GAAATCCAAA GCAGAGGCCG GGGGTCAAAC GACGAGATTG TGAAGAATCT 
AATATGGATA TATTTGATGC CGACAGTGCC AAAGCACCTC ACTATGTGCT TTCTCAGCTT 
ACCACGGACA ACAAAGGCAA CTCAAAAGCG GGAAATGGAA CATTGGAAAA CCAAAAAGGA 
ACTGGAGTAA AGAAGAGCCC TATGTTGTGT GGACAATATC CTGTTAAAAG TGAGGGAAAG 
GAGCTGAAGA TAGTTGTACA ACCTGAGACA CAGCACCGAG CTCGGTACCT GACTGAGGGC 
AGCCGTGGCT CAGTGAAAGA TAGAACACAG CAAGGCTTTC CTACAGTAAA GCTGGAAGGC 
CATAATGAAC CTGTAGTGTT GCAAGTGTTT GTGGGCAACG ACTCTGGACG AGTGAAACCA 
CATGGATTTT ATCAGGCCTG CAGAGTAACT GGACGAAATA CAACTCCTTG CAAAGAAGTG 
GACATTGAAG GCACTACTGT TATAGAAGTC GGCCTTGATC CTAGCAACAA CATGACACTG 
GCGGTGGACT GCGTAGGGAT ATTGAAATTG AGGAATGCTG ATGTCGAAGC CAGAATAGGA 
ATTGCTGGTT CCAAGAAGAA AAGCACTCGT GCCAGATTGG TTTTTCGAGT TAATATCATG 
AGGAAAGATG GCTCCACTTT GACACTGCAA ACACCCTCTT CTCCAATTTT GTGTACTCAG 
CCAGCAGGAG TGCCAGAAAT CTTAAAGAAA AGCTTGCATA GCTGTTCAGT GAAAGGAGAA 
GAAGAAGTGT TTTTAATCGG CAAGAACTTT CTGAAAGGAA CTAAAGTTAT TTTCCAAGAA 
AATGTTTCTG ATGAAAACTC TTGGAAGTCA GAAGCTGAAA TTGATATGGA ACTATTTCAT 
CAGAATCATC TTATTGTGAA GGTTCCTCCC TATCATGACC AACATATAAC TTTGCCTGTG 
TCAGTGGGAA TATATGTAGT GACAAATGCT GGAAGATCTC ATGATGTTCA ACCATTCACT 
TACACTCCAG ACCCAGCAGC AGCTGGTGCT TTGAATGTAA ATGTGAAGAA GGAAATATCT 
AGTCCAGCAA GACCTTGCTC TTTTGAAGAG GCCATGAAAG CAATGAAAAC TACTGGATGT 
AATTTAGATA AGGTAAATAT TATCCCTAAT GCCCTGATGA CTCCACTCAT ACCAAGCAGT 
ATGATTAAGA GTGAAGATGT TACTCCAATG GAAGTAACAG CAGAAAAAAG ATCTTCCACT 
ATTTTTAAGA CTACAAAGTC TGTTGGATCA ACTCAGCAAA CATTAGAAAA CATCTCAAAC 
ATAGCAGGAA ATGGCTCTTT TTCATCACCA TCATCTTCCC ACCTACCTTC TGAAAATGAA 
AAACAGCAGC AGATTCAGCC CAAGGCATAC AACCCAGAGA CCCTGACAAC TATTCAAACC 
CAGGACATCT CACAGCCTGG TACTTTTCCA GCAGTTTCTG CTTCTAGTCA GCTGCCCAAC 
AGCGATGCAC TATTGCAGCA GGCTACACAG TTTCAGACAA GAGAAACTCA GTCTAGAGAG 
ATATTACAGT CAGATGGTAC AGTGGTTAAT TTGTCACAAC TGACTGAGGC ATCACAACAA 
CAGCAGCAGT CACCACTACA AGAACAAGCA CAGACTTTAC AGCAGCAGAT TTCATCAAAT 
ATTTTTCCAT CACCAAATAG TGTGAGTCAG CTTCAGAATA CTATTCAGCA GCTGCAAGCA 
GGGAGTTTCA CAGGCAGTAC TGCTAGTGGC AGCAGTGGAA GTGTTGACTT GGTCCAACAA 
GTTTTAGAGG CACAGCAGCA GTTATCTTCA GTTTTATTTT CTGCTCCAGA TGGTAATGAG 
AATGTTCAAG AGCAGCTTAG TGCAGATATT TTTCAACAAG TCAGTCAAAT TCAGAGTGGT 
GTAAGCCCTG GAATGTTTTC CTCAACAGAG CCAACAGTCC ATACCAGACC AGATAATTTA 
TTACCTGGAA GAGCTGAAAG TGTTCATCCA CAGTCTGAAA ACACGTTATC TAATCAACAG 
CAGCAGCAGC AGCAGCAACA GCAAGTGATG GAATCTTCAG CCGCAATGGT GATGGAGATG 
CAACAGAGTA TCTGCCAGGC AGCTGCCCAG ATTCAGTCAG AGTTATTCCC TTCAACTGCT 
TCAGCAAATG GAAACCTTCA GCAATCGCCA GTTTACCAGC AGACTTCTCA CATGATGAGT 
GCATTGTCTA CCAATGAGGA TATGCAAATG CAGTGTGAAT TGTTTTCTTC TCCTCCTGCA 
GTTTCTGGAA ATGAAACTTC TACAACTACC ACACAGCAGG TTGCAACCCC TGGCACTACC 
ATGTTTCAGA CATCAAGTTC AGGAGATGGA GAAGAAACTG GAACACAAGC AAAACAGATT 
CAGAACAGTG TCTTTCAGAC CATGGTCCAA ATGCAACATA GTGGGGACAA TCAACCTCAA 
GTTAACCTTT TTTCATCCAC AAAAAGTATG ATGAGTGTTC AGAATAGTGG TACCCAACAA 
CAAGGTAATG GTTTATTCCA GCAAGGGAAT GAGATGATGT CACTTCAATC TGGAAATTTT 
TTGCAGCAGT CTTCTCATTC ACAGGCCCAA CTTTTTCATC CTCAAAATCC TATTGCCGAT 
GCTCAGAACC TTTCCCAGGA AACTCAAGGT TCTCTCTTTC ATAGTCCAAA TCCTATTGTC 
CACAGTCAGA CTTCTACAAC CTCCTCTGAA CAAATGCAGC CTCCAATGTT TCACTCTCAA 
AGTACCATTG CTGTGTTACA GGGCTCTTCA GTTCCTCAAG ACCAGCAGTC AACCAACATA 
TTTCTTTCCC AGAGTCCCAT GAATAATCTT CAGACTAACA CAGTAGCCCA AGAAGCATTT 
TTTGCAGCAC CGAACTCAAT TTCTCCACTT CAGTCAACAT CAAACAGTGA ACAACAAGCT 
GCTTTCCAAC AGCAAGCTCC AATATCACAC ATCCAGACTC CTATGCTTTC CCAAGAACAG 
GCACAACCCC CGCAGCAGGG TTTATTTCAG CCTCAGGTGG CCCTGGGCTC CCTTCCACCT 
AATCCAATGC CTCAAAGCCA ACAAGGAACC ATGTTCCAGT CACAGCACTC AATAGTTGCC 
ATGCAGAGTA ACTCTCCATC CCAGGAACAG CAGCAGCAGC AGCAACAGCA GCAGCAACAG 
CAGCAGCAAC AACAACAGAG CATTTTATTC AGTAATCAGA ATACCATGGC TACAATGGCG 
TCTCCAAAGC AACCACCACC AAACATGATA TTCAACCCAA ATCAAAATCC AATGGCTAAT 
CAGGAGCAAC AGAACCAGTC AATTTTTCAC CAACAAAGTA ACATGGCCCC AATGAATCAA 
GAGCAACAGC CCATGCAATT TCAGAGTCAG TCCACAGTTT CCTCACTTCA GAACCCAGGT 
CCTACCCAGT CGGAATCATC ACAGACCCCC TTGTTCCATA GCTCTCCTCA GATTCAGTTG 
GTACAAGGGT CACCTAGTTC TCAAGAGCAG CAAGTAACTC TCTTCTTATC TCCAGCATCC 
ATGTCTGCCT TGCAGACCAG TATAAATCAA CAAGATATGC AACAGTCTCC TCTTTATTCC 
CCTCAGAACA ACATGCCTGG AATTCAAGGA GCCACATCTT CGCCTCAACC ACAGGCTACT 
TTATTTCACA ACACAGCAGG AGGCACAATG AACCAACTGC AGAATTCTCC TGGCTCATCT 
CAGCAGACAT CAGGAATGTT CTTATTTGGC ATTCAAAATA ACTGTAGTCA GCTTTTAACC 
TCTGGACCAG CTACATTGCC TGATCAGTTG ATGGCCATAA GTCAGCCAGG CCAACCACAA 
AACGAGGGCC AGCCACCTGT GACAACACTT CTTTCTCAGC AAATGCCAGA GAATTCTCCA 
CTGGCATCCT CTATAAACAC CAACCAGAAC ATCGAAAAGA TTGATTTGCT TGTTTCATTG 
CAAAACCAAG GGAACAACTT GACTGGCTCC TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( NM_006599.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006590.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu11182
Clone ID Related Accession (Same CDS sequence)	NM_006599.4 , NM_006599.3
Accession Version	NM_006599.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009032.8, AB020634.1, AC012321.8 and AA291524.1. On Aug 4, 2011 this sequence version replaced NM_006599.2. Transcript Variant: This variant (3) lacks two exons in the 5' region, compared to variant 1. This difference causes translation initiation at an upstream start codon and results in an isoform (c) with a longer N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.164252.1, BC146765.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTCTACTCGC GAGAATCTGT CTATGATCTT CTCCCAAAGG AGTTACAGTT ACCTCCATCT 
AGAGAAACAT CTGTAGCATC AATGAGTCAG ACAAGCGGTG GTGAGGCAGG CTCGCCTCCT 
CCAGCTGTTG TTGCTGCTGA TGCTTCTTCA GCTCCCTCCT CTTCCTCCAT GGGCGGTGCT 
TGCAGCTCCT TTACCACCTC TTCCAGCCCT ACCATTTATT CTACCTCAGT CACCGACAGC 
AAGGCTATGC AAGTGGAGAG CTGCTCCTCA GCCGTGGGGG TAAGTAACAG AGGGGTAAGT 
GAAAAGCAGT TAACCAGTAA CACAGTTCAG CAGCATCCAT CAACACCGAA GAGGCACACA 
GTCTTGTACA TCTCACCACC ACCTGAGGAC TTGCTGGATA ACAGTCGGAT GTCCTGCCAG 
GATGAGGGGT GTGGATTGGA ATCTGAGCAG AGCTGCAGTA TGTGGATGGA GGATTCCCCC 
TCCAACTTCA GTAACATGAG CACCAGTTCC TACAATGATA ACACTGAGGT ACCTCGTAAA 
TCACGAAAAC GAAATCCAAA GCAGAGGCCG GGGGTCAAAC GACGAGATTG TGAAGAATCT 
AATATGGATA TATTTGATGC CGACAGTGCC AAAGCACCTC ACTATGTGCT TTCTCAGCTT 
ACCACGGACA ACAAAGGCAA CTCAAAAGCG GGAAATGGAA CATTGGAAAA CCAAAAAGGA 
ACTGGAGTAA AGAAGAGCCC TATGTTGTGT GGACAATATC CTGTTAAAAG TGAGGGAAAG 
GAGCTGAAGA TAGTTGTACA ACCTGAGACA CAGCACCGAG CTCGGTACCT GACTGAGGGC 
AGCCGTGGCT CAGTGAAAGA TAGAACACAG CAAGGCTTTC CTACAGTAAA GCTGGAAGGC 
CATAATGAAC CTGTAGTGTT GCAAGTGTTT GTGGGCAACG ACTCTGGACG AGTGAAACCA 
CATGGATTTT ATCAGGCCTG CAGAGTAACT GGACGAAATA CAACTCCTTG CAAAGAAGTG 
GACATTGAAG GCACTACTGT TATAGAAGTC GGCCTTGATC CTAGCAACAA CATGACACTG 
GCGGTGGACT GCGTAGGGAT ATTGAAATTG AGGAATGCTG ATGTCGAAGC CAGAATAGGA 
ATTGCTGGTT CCAAGAAGAA AAGCACTCGT GCCAGATTGG TTTTTCGAGT TAATATCATG 
AGGAAAGATG GCTCCACTTT GACACTGCAA ACACCCTCTT CTCCAATTTT GTGTACTCAG 
CCAGCAGGAG TGCCAGAAAT CTTAAAGAAA AGCTTGCATA GCTGTTCAGT GAAAGGAGAA 
GAAGAAGTGT TTTTAATCGG CAAGAACTTT CTGAAAGGAA CTAAAGTTAT TTTCCAAGAA 
AATGTTTCTG ATGAAAACTC TTGGAAGTCA GAAGCTGAAA TTGATATGGA ACTATTTCAT 
CAGAATCATC TTATTGTGAA GGTTCCTCCC TATCATGACC AACATATAAC TTTGCCTGTG 
TCAGTGGGAA TATATGTAGT GACAAATGCT GGAAGATCTC ATGATGTTCA ACCATTCACT 
TACACTCCAG ACCCAGCAGC AGCTGGTGCT TTGAATGTAA ATGTGAAGAA GGAAATATCT 
AGTCCAGCAA GACCTTGCTC TTTTGAAGAG GCCATGAAAG CAATGAAAAC TACTGGATGT 
AATTTAGATA AGGTAAATAT TATCCCTAAT GCCCTGATGA CTCCACTCAT ACCAAGCAGT 
ATGATTAAGA GTGAAGATGT TACTCCAATG GAAGTAACAG CAGAAAAAAG ATCTTCCACT 
ATTTTTAAGA CTACAAAGTC TGTTGGATCA ACTCAGCAAA CATTAGAAAA CATCTCAAAC 
ATAGCAGGAA ATGGCTCTTT TTCATCACCA TCATCTTCCC ACCTACCTTC TGAAAATGAA 
AAACAGCAGC AGATTCAGCC CAAGGCATAC AACCCAGAGA CCCTGACAAC TATTCAAACC 
CAGGACATCT CACAGCCTGG TACTTTTCCA GCAGTTTCTG CTTCTAGTCA GCTGCCCAAC 
AGCGATGCAC TATTGCAGCA GGCTACACAG TTTCAGACAA GAGAAACTCA GTCTAGAGAG 
ATATTACAGT CAGATGGTAC AGTGGTTAAT TTGTCACAAC TGACTGAGGC ATCACAACAA 
CAGCAGCAGT CACCACTACA AGAACAAGCA CAGACTTTAC AGCAGCAGAT TTCATCAAAT 
ATTTTTCCAT CACCAAATAG TGTGAGTCAG CTTCAGAATA CTATTCAGCA GCTGCAAGCA 
GGGAGTTTCA CAGGCAGTAC TGCTAGTGGC AGCAGTGGAA GTGTTGACTT GGTCCAACAA 
GTTTTAGAGG CACAGCAGCA GTTATCTTCA GTTTTATTTT CTGCTCCAGA TGGTAATGAG 
AATGTTCAAG AGCAGCTTAG TGCAGATATT TTTCAACAAG TCAGTCAAAT TCAGAGTGGT 
GTAAGCCCTG GAATGTTTTC CTCAACAGAG CCAACAGTCC ATACCAGACC AGATAATTTA 
TTACCTGGAA GAGCTGAAAG TGTTCATCCA CAGTCTGAAA ACACGTTATC TAATCAACAG 
CAGCAGCAGC AGCAGCAACA GCAAGTGATG GAATCTTCAG CCGCAATGGT GATGGAGATG 
CAACAGAGTA TCTGCCAGGC AGCTGCCCAG ATTCAGTCAG AGTTATTCCC TTCAACTGCT 
TCAGCAAATG GAAACCTTCA GCAATCGCCA GTTTACCAGC AGACTTCTCA CATGATGAGT 
GCATTGTCTA CCAATGAGGA TATGCAAATG CAGTGTGAAT TGTTTTCTTC TCCTCCTGCA 
GTTTCTGGAA ATGAAACTTC TACAACTACC ACACAGCAGG TTGCAACCCC TGGCACTACC 
ATGTTTCAGA CATCAAGTTC AGGAGATGGA GAAGAAACTG GAACACAAGC AAAACAGATT 
CAGAACAGTG TCTTTCAGAC CATGGTCCAA ATGCAACATA GTGGGGACAA TCAACCTCAA 
GTTAACCTTT TTTCATCCAC AAAAAGTATG ATGAGTGTTC AGAATAGTGG TACCCAACAA 
CAAGGTAATG GTTTATTCCA GCAAGGGAAT GAGATGATGT CACTTCAATC TGGAAATTTT 
TTGCAGCAGT CTTCTCATTC ACAGGCCCAA CTTTTTCATC CTCAAAATCC TATTGCCGAT 
GCTCAGAACC TTTCCCAGGA AACTCAAGGT TCTCTCTTTC ATAGTCCAAA TCCTATTGTC 
CACAGTCAGA CTTCTACAAC CTCCTCTGAA CAAATGCAGC CTCCAATGTT TCACTCTCAA 
AGTACCATTG CTGTGTTACA GGGCTCTTCA GTTCCTCAAG ACCAGCAGTC AACCAACATA 
TTTCTTTCCC AGAGTCCCAT GAATAATCTT CAGACTAACA CAGTAGCCCA AGAAGCATTT 
TTTGCAGCAC CGAACTCAAT TTCTCCACTT CAGTCAACAT CAAACAGTGA ACAACAAGCT 
GCTTTCCAAC AGCAAGCTCC AATATCACAC ATCCAGACTC CTATGCTTTC CCAAGAACAG 
GCACAACCCC CGCAGCAGGG TTTATTTCAG CCTCAGGTGG CCCTGGGCTC CCTTCCACCT 
AATCCAATGC CTCAAAGCCA ACAAGGAACC ATGTTCCAGT CACAGCACTC AATAGTTGCC 
ATGCAGAGTA ACTCTCCATC CCAGGAACAG CAGCAGCAGC AGCAACAGCA GCAGCAACAG 
CAGCAGCAAC AACAACAGAG CATTTTATTC AGTAATCAGA ATACCATGGC TACAATGGCG 
TCTCCAAAGC AACCACCACC AAACATGATA TTCAACCCAA ATCAAAATCC AATGGCTAAT 
CAGGAGCAAC AGAACCAGTC AATTTTTCAC CAACAAAGTA ACATGGCCCC AATGAATCAA 
GAGCAACAGC CCATGCAATT TCAGAGTCAG TCCACAGTTT CCTCACTTCA GAACCCAGGT 
CCTACCCAGT CGGAATCATC ACAGACCCCC TTGTTCCATA GCTCTCCTCA GATTCAGTTG 
GTACAAGGGT CACCTAGTTC TCAAGAGCAG CAAGTAACTC TCTTCTTATC TCCAGCATCC 
ATGTCTGCCT TGCAGACCAG TATAAATCAA CAAGATATGC AACAGTCTCC TCTTTATTCC 
CCTCAGAACA ACATGCCTGG AATTCAAGGA GCCACATCTT CGCCTCAACC ACAGGCTACT 
TTATTTCACA ACACAGCAGG AGGCACAATG AACCAACTGC AGAATTCTCC TGGCTCATCT 
CAGCAGACAT CAGGAATGTT CTTATTTGGC ATTCAAAATA ACTGTAGTCA GCTTTTAACC 
TCTGGACCAG CTACATTGCC TGATCAGTTG ATGGCCATAA GTCAGCCAGG CCAACCACAA 
AACGAGGGCC AGCCACCTGT GACAACACTT CTTTCTCAGC AAATGCCAGA GAATTCTCCA 
CTGGCATCCT CTATAAACAC CAACCAGAAC ATCGAAAAGA TTGATTTGCT TGTTTCATTG 
CAAAACCAAG GGAACAACTT GACTGGCTCC TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006590.1
CDS	337..4932
Translation	MPSDFISLLSADLDLESPKSLYSRESVYDLLPKELQLPPSRETSVASMSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	NM_006599.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006599.3

ORF Insert Sequence:

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTCTACTCGC GAGAATCTGT CTATGATCTT CTCCCAAAGG AGTTACAGTT ACCTCCATCT 
AGAGAAACAT CTGTAGCATC AATGAGTCAG ACAAGCGGTG GTGAGGCAGG CTCGCCTCCT 
CCAGCTGTTG TTGCTGCTGA TGCTTCTTCA GCTCCCTCCT CTTCCTCCAT GGGCGGTGCT 
TGCAGCTCCT TTACCACCTC TTCCAGCCCT ACCATTTATT CTACCTCAGT CACCGACAGC 
AAGGCTATGC AAGTGGAGAG CTGCTCCTCA GCCGTGGGGG TAAGTAACAG AGGGGTAAGT 
GAAAAGCAGT TAACCAGTAA CACAGTTCAG CAGCATCCAT CAACACCGAA GAGGCACACA 
GTCTTGTACA TCTCACCACC ACCTGAGGAC TTGCTGGATA ACAGTCGGAT GTCCTGCCAG 
GATGAGGGGT GTGGATTGGA ATCTGAGCAG AGCTGCAGTA TGTGGATGGA GGATTCCCCC 
TCCAACTTCA GTAACATGAG CACCAGTTCC TACAATGATA ACACTGAGGT ACCTCGTAAA 
TCACGAAAAC GAAATCCAAA GCAGAGGCCG GGGGTCAAAC GACGAGATTG TGAAGAATCT 
AATATGGATA TATTTGATGC CGACAGTGCC AAAGCACCTC ACTATGTGCT TTCTCAGCTT 
ACCACGGACA ACAAAGGCAA CTCAAAAGCG GGAAATGGAA CATTGGAAAA CCAAAAAGGA 
ACTGGAGTAA AGAAGAGCCC TATGTTGTGT GGACAATATC CTGTTAAAAG TGAGGGAAAG 
GAGCTGAAGA TAGTTGTACA ACCTGAGACA CAGCACCGAG CTCGGTACCT GACTGAGGGC 
AGCCGTGGCT CAGTGAAAGA TAGAACACAG CAAGGCTTTC CTACAGTAAA GCTGGAAGGC 
CATAATGAAC CTGTAGTGTT GCAAGTGTTT GTGGGCAACG ACTCTGGACG AGTGAAACCA 
CATGGATTTT ATCAGGCCTG CAGAGTAACT GGACGAAATA CAACTCCTTG CAAAGAAGTG 
GACATTGAAG GCACTACTGT TATAGAAGTC GGCCTTGATC CTAGCAACAA CATGACACTG 
GCGGTGGACT GCGTAGGGAT ATTGAAATTG AGGAATGCTG ATGTCGAAGC CAGAATAGGA 
ATTGCTGGTT CCAAGAAGAA AAGCACTCGT GCCAGATTGG TTTTTCGAGT TAATATCATG 
AGGAAAGATG GCTCCACTTT GACACTGCAA ACACCCTCTT CTCCAATTTT GTGTACTCAG 
CCAGCAGGAG TGCCAGAAAT CTTAAAGAAA AGCTTGCATA GCTGTTCAGT GAAAGGAGAA 
GAAGAAGTGT TTTTAATCGG CAAGAACTTT CTGAAAGGAA CTAAAGTTAT TTTCCAAGAA 
AATGTTTCTG ATGAAAACTC TTGGAAGTCA GAAGCTGAAA TTGATATGGA ACTATTTCAT 
CAGAATCATC TTATTGTGAA GGTTCCTCCC TATCATGACC AACATATAAC TTTGCCTGTG 
TCAGTGGGAA TATATGTAGT GACAAATGCT GGAAGATCTC ATGATGTTCA ACCATTCACT 
TACACTCCAG ACCCAGCAGC AGCTGGTGCT TTGAATGTAA ATGTGAAGAA GGAAATATCT 
AGTCCAGCAA GACCTTGCTC TTTTGAAGAG GCCATGAAAG CAATGAAAAC TACTGGATGT 
AATTTAGATA AGGTAAATAT TATCCCTAAT GCCCTGATGA CTCCACTCAT ACCAAGCAGT 
ATGATTAAGA GTGAAGATGT TACTCCAATG GAAGTAACAG CAGAAAAAAG ATCTTCCACT 
ATTTTTAAGA CTACAAAGTC TGTTGGATCA ACTCAGCAAA CATTAGAAAA CATCTCAAAC 
ATAGCAGGAA ATGGCTCTTT TTCATCACCA TCATCTTCCC ACCTACCTTC TGAAAATGAA 
AAACAGCAGC AGATTCAGCC CAAGGCATAC AACCCAGAGA CCCTGACAAC TATTCAAACC 
CAGGACATCT CACAGCCTGG TACTTTTCCA GCAGTTTCTG CTTCTAGTCA GCTGCCCAAC 
AGCGATGCAC TATTGCAGCA GGCTACACAG TTTCAGACAA GAGAAACTCA GTCTAGAGAG 
ATATTACAGT CAGATGGTAC AGTGGTTAAT TTGTCACAAC TGACTGAGGC ATCACAACAA 
CAGCAGCAGT CACCACTACA AGAACAAGCA CAGACTTTAC AGCAGCAGAT TTCATCAAAT 
ATTTTTCCAT CACCAAATAG TGTGAGTCAG CTTCAGAATA CTATTCAGCA GCTGCAAGCA 
GGGAGTTTCA CAGGCAGTAC TGCTAGTGGC AGCAGTGGAA GTGTTGACTT GGTCCAACAA 
GTTTTAGAGG CACAGCAGCA GTTATCTTCA GTTTTATTTT CTGCTCCAGA TGGTAATGAG 
AATGTTCAAG AGCAGCTTAG TGCAGATATT TTTCAACAAG TCAGTCAAAT TCAGAGTGGT 
GTAAGCCCTG GAATGTTTTC CTCAACAGAG CCAACAGTCC ATACCAGACC AGATAATTTA 
TTACCTGGAA GAGCTGAAAG TGTTCATCCA CAGTCTGAAA ACACGTTATC TAATCAACAG 
CAGCAGCAGC AGCAGCAACA GCAAGTGATG GAATCTTCAG CCGCAATGGT GATGGAGATG 
CAACAGAGTA TCTGCCAGGC AGCTGCCCAG ATTCAGTCAG AGTTATTCCC TTCAACTGCT 
TCAGCAAATG GAAACCTTCA GCAATCGCCA GTTTACCAGC AGACTTCTCA CATGATGAGT 
GCATTGTCTA CCAATGAGGA TATGCAAATG CAGTGTGAAT TGTTTTCTTC TCCTCCTGCA 
GTTTCTGGAA ATGAAACTTC TACAACTACC ACACAGCAGG TTGCAACCCC TGGCACTACC 
ATGTTTCAGA CATCAAGTTC AGGAGATGGA GAAGAAACTG GAACACAAGC AAAACAGATT 
CAGAACAGTG TCTTTCAGAC CATGGTCCAA ATGCAACATA GTGGGGACAA TCAACCTCAA 
GTTAACCTTT TTTCATCCAC AAAAAGTATG ATGAGTGTTC AGAATAGTGG TACCCAACAA 
CAAGGTAATG GTTTATTCCA GCAAGGGAAT GAGATGATGT CACTTCAATC TGGAAATTTT 
TTGCAGCAGT CTTCTCATTC ACAGGCCCAA CTTTTTCATC CTCAAAATCC TATTGCCGAT 
GCTCAGAACC TTTCCCAGGA AACTCAAGGT TCTCTCTTTC ATAGTCCAAA TCCTATTGTC 
CACAGTCAGA CTTCTACAAC CTCCTCTGAA CAAATGCAGC CTCCAATGTT TCACTCTCAA 
AGTACCATTG CTGTGTTACA GGGCTCTTCA GTTCCTCAAG ACCAGCAGTC AACCAACATA 
TTTCTTTCCC AGAGTCCCAT GAATAATCTT CAGACTAACA CAGTAGCCCA AGAAGCATTT 
TTTGCAGCAC CGAACTCAAT TTCTCCACTT CAGTCAACAT CAAACAGTGA ACAACAAGCT 
GCTTTCCAAC AGCAAGCTCC AATATCACAC ATCCAGACTC CTATGCTTTC CCAAGAACAG 
GCACAACCCC CGCAGCAGGG TTTATTTCAG CCTCAGGTGG CCCTGGGCTC CCTTCCACCT 
AATCCAATGC CTCAAAGCCA ACAAGGAACC ATGTTCCAGT CACAGCACTC AATAGTTGCC 
ATGCAGAGTA ACTCTCCATC CCAGGAACAG CAGCAGCAGC AGCAACAGCA GCAGCAACAG 
CAGCAGCAAC AACAACAGAG CATTTTATTC AGTAATCAGA ATACCATGGC TACAATGGCG 
TCTCCAAAGC AACCACCACC AAACATGATA TTCAACCCAA ATCAAAATCC AATGGCTAAT 
CAGGAGCAAC AGAACCAGTC AATTTTTCAC CAACAAAGTA ACATGGCCCC AATGAATCAA 
GAGCAACAGC CCATGCAATT TCAGAGTCAG TCCACAGTTT CCTCACTTCA GAACCCAGGT 
CCTACCCAGT CGGAATCATC ACAGACCCCC TTGTTCCATA GCTCTCCTCA GATTCAGTTG 
GTACAAGGGT CACCTAGTTC TCAAGAGCAG CAAGTAACTC TCTTCTTATC TCCAGCATCC 
ATGTCTGCCT TGCAGACCAG TATAAATCAA CAAGATATGC AACAGTCTCC TCTTTATTCC 
CCTCAGAACA ACATGCCTGG AATTCAAGGA GCCACATCTT CGCCTCAACC ACAGGCTACT 
TTATTTCACA ACACAGCAGG AGGCACAATG AACCAACTGC AGAATTCTCC TGGCTCATCT 
CAGCAGACAT CAGGAATGTT CTTATTTGGC ATTCAAAATA ACTGTAGTCA GCTTTTAACC 
TCTGGACCAG CTACATTGCC TGATCAGTTG ATGGCCATAA GTCAGCCAGG CCAACCACAA 
AACGAGGGCC AGCCACCTGT GACAACACTT CTTTCTCAGC AAATGCCAGA GAATTCTCCA 
CTGGCATCCT CTATAAACAC CAACCAGAAC ATCGAAAAGA TTGATTTGCT TGTTTCATTG 
CAAAACCAAG GGAACAACTT GACTGGCTCC TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( NM_001367709.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354638.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu85815
Clone ID Related Accession (Same CDS sequence)	NM_001367709.1 , XM_017022873.1
Accession Version	NM_001367709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009032.8 and AC012321.8. On Dec 12, 2018 this sequence version replaced XM_017022873.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.278063.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCTGGTGCTT TGAATGTAAA TGTGAAGAAG GAAATATCTA GTCCAGCAAG ACCTTGCTCT 
TTTGAAGAGG CCATGAAAGC AATGAAAACT ACTGGATGTA ATTTAGATAA GGTAAATATT 
ATCCCTAATG CCCTGATGAC TCCACTCATA CCAAGCAGTA TGATTAAGAG TGAAGATGTT 
ACTCCAATGG AAGTAACAGC AGAAAAAAGA TCTTCCACTA TTTTTAAGAC TACAAAGTCT 
GTTGGATCAA CTCAGCAAAC ATTAGAAAAC ATCTCAAACA TAGCAGGAAA TGGCTCTTTT 
TCATCACCAT CATCTTCCCA CCTACCTTCT GAAAATGAAA AACAGCAGCA GATTCAGCCC 
AAGGCATACA ACCCAGAGAC CCTGACAACT ATTCAAACCC AGGACATCTC ACAGCCTGGT 
ACTTTTCCAG CAGTTTCTGC TTCTAGTCAG CTGCCCAACA GCGATGCACT ATTGCAGCAG 
GCTACACAGT TTCAGACAAG AGAAACTCAG TCTAGAGAGA TATTACAGTC AGATGGTACA 
GTGGTTAATT TGTCACAACT GACTGAGGCA TCACAACAAC AGCAGCAGTC ACCACTACAA 
GAACAAGCAC AGACTTTACA GCAGCAGATT TCATCAAATA TTTTTCCATC ACCAAATAGT 
GTGAGTCAGC TTCAGAATAC TATTCAGCAG CTGCAAGCAG GGAGTTTCAC AGGCAGTACT 
GCTAGTGGCA GCAGTGGAAG TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAGCAGCAG 
TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAATGAGA ATGTTCAAGA GCAGCTTAGT 
GCAGATATTT TTCAACAAGT CAGTCAAATT CAGAGTGGTG TAAGCCCTGG AATGTTTTCC 
TCAACAGAGC CAACAGTCCA TACCAGACCA GATAATTTAT TACCTGGAAG AGCTGAAAGT 
GTTCATCCAC AGTCTGAAAA CACGTTATCT AATCAACAGC AGCAGCAGCA GCAGCAACAG 
CAAGTGATGG AATCTTCAGC CGCAATGGTG ATGGAGATGC AACAGAGTAT CTGCCAGGCA 
GCTGCCCAGA TTCAGTCAGA GTTATTCCCT TCAACTGCTT CAGCAAATGG AAACCTTCAG 
CAATCGCCAG TTTACCAGCA GACTTCTCAC ATGATGAGTG CATTGTCTAC CAATGAGGAT 
ATGCAAATGC AGTGTGAATT GTTTTCTTCT CCTCCTGCAG TTTCTGGAAA TGAAACTTCT 
ACAACTACCA CACAGCAGGT TGCAACCCCT GGCACTACCA TGTTTCAGAC ATCAAGTTCA 
GGAGATGGAG AAGAAACTGG AACACAAGCA AAACAGATTC AGAACAGTGT CTTTCAGACC 
ATGGTCCAAA TGCAACATAG TGGGGACAAT CAACCTCAAG TTAACCTTTT TTCATCCACA 
AAAAGTATGA TGAGTGTTCA GAATAGTGGT ACCCAACAAC AAGGTAATGG TTTATTCCAG 
CAAGGGAATG AGATGATGTC ACTTCAATCT GGAAATTTTT TGCAGCAGTC TTCTCATTCA 
CAGGCCCAAC TTTTTCATCC TCAAAATCCT ATTGCCGATG CTCAGAACCT TTCCCAGGAA 
ACTCAAGGTT CTCTCTTTCA TAGTCCAAAT CCTATTGTCC ACAGTCAGAC TTCTACAACC 
TCCTCTGAAC AAATGCAGCC TCCAATGTTT CACTCTCAAA GTACCATTGC TGTGTTACAG 
GGCTCTTCAG TTCCTCAAGA CCAGCAGTCA ACCAACATAT TTCTTTCCCA GAGTCCCATG 
AATAATCTTC AGACTAACAC AGTAGCCCAA GAAGCATTTT TTGCAGCACC GAACTCAATT 
TCTCCACTTC AGTCAACATC AAACAGTGAA CAACAAGCTG CTTTCCAACA GCAAGCTCCA 
ATATCACACA TCCAGACTCC TATGCTTTCC CAAGAACAGG CACAACCCCC GCAGCAGGGT 
TTATTTCAGC CTCAGGTGGC CCTGGGCTCC CTTCCACCTA ATCCAATGCC TCAAAGCCAA 
CAAGGAACCA TGTTCCAGTC ACAGCACTCA ATAGTTGCCA TGCAGAGTAA CTCTCCATCC 
CAGGAACAGC AGCAGCAGCA GCAACAGCAG CAGCAACAGC AGCAGCAACA ACAACAGAGC 
ATTTTATTCA GTAATCAGAA TACCATGGCT ACAATGGCGT CTCCAAAGCA ACCACCACCA 
AACATGATAT TCAACCCAAA TCAAAATCCA ATGGCTAATC AGGAGCAACA GAACCAGTCA 
ATTTTTCACC AACAAAGTAA CATGGCCCCA ATGAATCAAG AGCAACAGCC CATGCAATTT 
CAGAGTCAGT CCACAGTTTC CTCACTTCAG AACCCAGGTC CTACCCAGTC GGAATCATCA 
CAGACCCCCT TGTTCCATAG CTCTCCTCAG ATTCAGTTGG TACAAGGGTC ACCTAGTTCT 
CAAGAGCAGC AAGTAACTCT CTTCTTATCT CCAGCATCCA TGTCTGCCTT GCAGACCAGT 
ATAAATCAAC AAGATATGCA ACAGTCTCCT CTTTATTCCC CTCAGAACAA CATGCCTGGA 
ATTCAAGGAG CCACATCTTC GCCTCAACCA CAGGCTACTT TATTTCACAA CACAGCAGGA 
GGCACAATGA ACCAACTGCA GAATTCTCCT GGCTCATCTC AGCAGACATC AGGAATGTTC 
TTATTTGGCA TTCAAAATAA CTGTAGTCAG CTTTTAACCT CTGGACCAGC TACATTGCCT 
GATCAGTTGA TGGCCATAAG TCAGCCAGGC CAACCACAAA ACGAGGGCCA GCCACCTGTG 
ACAACACTTC TTTCTCAGCA AATGCCAGAG AATTCTCCAC TGGCATCCTC TATAAACACC 
AACCAGAACA TCGAAAAGAT TGATTTGCTT GTTTCATTGC AAAACCAAGG GAACAACTTG 
ACTGGCTCCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354638.1
CDS	536..4510
Translation	MLLQLPPLPPWAVLAAPLPPLPALPFILPQSPTARLCKWRAAPQPWGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	NM_001367709.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367709.1

ORF Insert Sequence:

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCTGGTGCTT TGAATGTAAA TGTGAAGAAG GAAATATCTA GTCCAGCAAG ACCTTGCTCT 
TTTGAAGAGG CCATGAAAGC AATGAAAACT ACTGGATGTA ATTTAGATAA GGTAAATATT 
ATCCCTAATG CCCTGATGAC TCCACTCATA CCAAGCAGTA TGATTAAGAG TGAAGATGTT 
ACTCCAATGG AAGTAACAGC AGAAAAAAGA TCTTCCACTA TTTTTAAGAC TACAAAGTCT 
GTTGGATCAA CTCAGCAAAC ATTAGAAAAC ATCTCAAACA TAGCAGGAAA TGGCTCTTTT 
TCATCACCAT CATCTTCCCA CCTACCTTCT GAAAATGAAA AACAGCAGCA GATTCAGCCC 
AAGGCATACA ACCCAGAGAC CCTGACAACT ATTCAAACCC AGGACATCTC ACAGCCTGGT 
ACTTTTCCAG CAGTTTCTGC TTCTAGTCAG CTGCCCAACA GCGATGCACT ATTGCAGCAG 
GCTACACAGT TTCAGACAAG AGAAACTCAG TCTAGAGAGA TATTACAGTC AGATGGTACA 
GTGGTTAATT TGTCACAACT GACTGAGGCA TCACAACAAC AGCAGCAGTC ACCACTACAA 
GAACAAGCAC AGACTTTACA GCAGCAGATT TCATCAAATA TTTTTCCATC ACCAAATAGT 
GTGAGTCAGC TTCAGAATAC TATTCAGCAG CTGCAAGCAG GGAGTTTCAC AGGCAGTACT 
GCTAGTGGCA GCAGTGGAAG TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAGCAGCAG 
TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAATGAGA ATGTTCAAGA GCAGCTTAGT 
GCAGATATTT TTCAACAAGT CAGTCAAATT CAGAGTGGTG TAAGCCCTGG AATGTTTTCC 
TCAACAGAGC CAACAGTCCA TACCAGACCA GATAATTTAT TACCTGGAAG AGCTGAAAGT 
GTTCATCCAC AGTCTGAAAA CACGTTATCT AATCAACAGC AGCAGCAGCA GCAGCAACAG 
CAAGTGATGG AATCTTCAGC CGCAATGGTG ATGGAGATGC AACAGAGTAT CTGCCAGGCA 
GCTGCCCAGA TTCAGTCAGA GTTATTCCCT TCAACTGCTT CAGCAAATGG AAACCTTCAG 
CAATCGCCAG TTTACCAGCA GACTTCTCAC ATGATGAGTG CATTGTCTAC CAATGAGGAT 
ATGCAAATGC AGTGTGAATT GTTTTCTTCT CCTCCTGCAG TTTCTGGAAA TGAAACTTCT 
ACAACTACCA CACAGCAGGT TGCAACCCCT GGCACTACCA TGTTTCAGAC ATCAAGTTCA 
GGAGATGGAG AAGAAACTGG AACACAAGCA AAACAGATTC AGAACAGTGT CTTTCAGACC 
ATGGTCCAAA TGCAACATAG TGGGGACAAT CAACCTCAAG TTAACCTTTT TTCATCCACA 
AAAAGTATGA TGAGTGTTCA GAATAGTGGT ACCCAACAAC AAGGTAATGG TTTATTCCAG 
CAAGGGAATG AGATGATGTC ACTTCAATCT GGAAATTTTT TGCAGCAGTC TTCTCATTCA 
CAGGCCCAAC TTTTTCATCC TCAAAATCCT ATTGCCGATG CTCAGAACCT TTCCCAGGAA 
ACTCAAGGTT CTCTCTTTCA TAGTCCAAAT CCTATTGTCC ACAGTCAGAC TTCTACAACC 
TCCTCTGAAC AAATGCAGCC TCCAATGTTT CACTCTCAAA GTACCATTGC TGTGTTACAG 
GGCTCTTCAG TTCCTCAAGA CCAGCAGTCA ACCAACATAT TTCTTTCCCA GAGTCCCATG 
AATAATCTTC AGACTAACAC AGTAGCCCAA GAAGCATTTT TTGCAGCACC GAACTCAATT 
TCTCCACTTC AGTCAACATC AAACAGTGAA CAACAAGCTG CTTTCCAACA GCAAGCTCCA 
ATATCACACA TCCAGACTCC TATGCTTTCC CAAGAACAGG CACAACCCCC GCAGCAGGGT 
TTATTTCAGC CTCAGGTGGC CCTGGGCTCC CTTCCACCTA ATCCAATGCC TCAAAGCCAA 
CAAGGAACCA TGTTCCAGTC ACAGCACTCA ATAGTTGCCA TGCAGAGTAA CTCTCCATCC 
CAGGAACAGC AGCAGCAGCA GCAACAGCAG CAGCAACAGC AGCAGCAACA ACAACAGAGC 
ATTTTATTCA GTAATCAGAA TACCATGGCT ACAATGGCGT CTCCAAAGCA ACCACCACCA 
AACATGATAT TCAACCCAAA TCAAAATCCA ATGGCTAATC AGGAGCAACA GAACCAGTCA 
ATTTTTCACC AACAAAGTAA CATGGCCCCA ATGAATCAAG AGCAACAGCC CATGCAATTT 
CAGAGTCAGT CCACAGTTTC CTCACTTCAG AACCCAGGTC CTACCCAGTC GGAATCATCA 
CAGACCCCCT TGTTCCATAG CTCTCCTCAG ATTCAGTTGG TACAAGGGTC ACCTAGTTCT 
CAAGAGCAGC AAGTAACTCT CTTCTTATCT CCAGCATCCA TGTCTGCCTT GCAGACCAGT 
ATAAATCAAC AAGATATGCA ACAGTCTCCT CTTTATTCCC CTCAGAACAA CATGCCTGGA 
ATTCAAGGAG CCACATCTTC GCCTCAACCA CAGGCTACTT TATTTCACAA CACAGCAGGA 
GGCACAATGA ACCAACTGCA GAATTCTCCT GGCTCATCTC AGCAGACATC AGGAATGTTC 
TTATTTGGCA TTCAAAATAA CTGTAGTCAG CTTTTAACCT CTGGACCAGC TACATTGCCT 
GATCAGTTGA TGGCCATAAG TCAGCCAGGC CAACCACAAA ACGAGGGCCA GCCACCTGTG 
ACAACACTTC TTTCTCAGCA AATGCCAGAG AATTCTCCAC TGGCATCCTC TATAAACACC 
AACCAGAACA TCGAAAAGAT TGATTTGCTT GTTTCATTGC AAAACCAAGG GAACAACTTG 
ACTGGCTCCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_006721125.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006721188.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X2, mRNA.

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CloneID	OHu49247
Clone ID Related Accession (Same CDS sequence)	XM_006721125.4 , XM_006721125.3
Accession Version	XM_006721125.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006721125.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGA CAAGCGGTGG TGAGGCAGGC TCGCCTCCTC CAGCTGTTGT TGCTGCTGAT 
GCTTCTTCAG CTCCCTCCTC TTCCTCCATG GGCGGTGCTT GCAGCTCCTT TACCACCTCT 
TCCAGCCCTA CCATTTATTC TACCTCAGTC ACCGACAGCA AGGCTATGCA AGTGGAGAGC 
TGCTCCTCAG CCGTGGGGGT AAGTAACAGA GGGGTAAGTG AAAAGCAGTT AACCAGTAAC 
ACAGTTCAGC AGCATCCATC AACACCGAAG AGGCACACAG TCTTGTACAT CTCACCACCA 
CCTGAGGACT TGCTGGATAA CAGTCGGATG TCCTGCCAGG ATGAGGGGTG TGGATTGGAA 
TCTGAGCAGA GCTGCAGTAT GTGGATGGAG GATTCCCCCT CCAACTTCAG TAACATGAGC 
ACCAGTTCCT ACAATGATAA CACTGAGGTA CCTCGTAAAT CACGAAAACG AAATCCAAAG 
CAGAGGCCGG GGGTCAAACG ACGAGATTGT GAAGAATCTA ATATGGATAT ATTTGATGCC 
GACAGTGCCA AAGCACCTCA CTATGTGCTT TCTCAGCTTA CCACGGACAA CAAAGGCAAC 
TCAAAAGCGG GAAATGGAAC ATTGGAAAAC CAAAAAGGAA CTGGAGTAAA GAAGAGCCCT 
ATGTTGTGTG GACAATATCC TGTTAAAAGT GAGGGAAAGG AGCTGAAGAT AGTTGTACAA 
CCTGAGACAC AGCACCGAGC TCGGTACCTG ACTGAGGGCA GCCGTGGCTC AGTGAAAGAT 
AGAACACAGC AAGGCTTTCC TACAGTAAAG CTGGAAGGCC ATAATGAACC TGTAGTGTTG 
CAAGTGTTTG TGGGCAACGA CTCTGGACGA GTGAAACCAC ATGGATTTTA TCAGGCCTGC 
AGAGTAACTG GACGAAATAC AACTCCTTGC AAAGAAGTGG ACATTGAAGG CACTACTGTT 
ATAGAAGTCG GCCTTGATCC TAGCAACAAC ATGACACTGG CGGTGGACTG CGTAGGGATA 
TTGAAATTGA GGAATGCTGA TGTCGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAGAAA 
AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCATGA GGAAAGATGG CTCCACTTTG 
ACACTGCAAA CACCCTCTTC TCCAATTTTG TGTACTCAGC CAGCAGGAGT GCCAGAAATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAAG AAGAAGTGTT TTTAATCGGC 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAGTCAG AAGCTGAAAT TGATATGGAA CTATTTCATC AGAATCATCT TATTGTGAAG 
GTTCCTCCCT ATCATGACCA ACATATAACT TTGCCTGTGT CAGTGGGAAT ATATGTAGTG 
ACAAATGCTG GAAGATCTCA TGATGTTCAA CCATTCACTT ACACTCCAGA CCCAGCAGCA 
GCTGGTGCTT TGAATGTAAA TGTGAAGAAG GAAATATCTA GTCCAGCAAG ACCTTGCTCT 
TTTGAAGAGG CCATGAAAGC AATGAAAACT ACTGGATGTA ATTTAGATAA GGTAAATATT 
ATCCCTAATG CCCTGATGAC TCCACTCATA CCAAGCAGTA TGATTAAGAG TGAAGATGTT 
ACTCCAATGG AAGTAACAGC AGAAAAAAGA TCTTCCACTA TTTTTAAGAC TACAAAGTCT 
GTTGGATCAA CTCAGCAAAC ATTAGAAAAC ATCTCAAACA TAGCAGGAAA TGGCTCTTTT 
TCATCACCAT CATCTTCCCA CCTACCTTCT GAAAATGAAA AACAGCAGCA GATTCAGCCC 
AAGGCATACA ACCCAGAGAC CCTGACAACT ATTCAAACCC AGGACATCTC ACAGCCTGGT 
ACTTTTCCAG CAGTTTCTGC TTCTAGTCAG CTGCCCAACA GCGATGCACT ATTGCAGCAG 
GCTACACAGT TTCAGACAAG AGAAACTCAG TCTAGAGAGA TATTACAGTC AGATGGTACA 
GTGGTTAATT TGTCACAACT GACTGAGGCA TCACAACAAC AGCAGCAGTC ACCACTACAA 
GAACAAGCAC AGACTTTACA GCAGCAGATT TCATCAAATA TTTTTCCATC ACCAAATAGT 
GTGAGTCAGC TTCAGAATAC TATTCAGCAG CTGCAAGCAG GGAGTTTCAC AGGCAGTACT 
GCTAGTGGCA GCAGTGGAAG TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAGCAGCAG 
TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAATGAGA ATGTTCAAGA GCAGCTTAGT 
GCAGATATTT TTCAACAAGT CAGTCAAATT CAGAGTGGTG TAAGCCCTGG AATGTTTTCC 
TCAACAGAGC CAACAGTCCA TACCAGACCA GATAATTTAT TACCTGGAAG AGCTGAAAGT 
GTTCATCCAC AGTCTGAAAA CACGTTATCT AATCAACAGC AGCAGCAGCA GCAGCAACAG 
CAAGTGATGG AATCTTCAGC CGCAATGGTG ATGGAGATGC AACAGAGTAT CTGCCAGGCA 
GCTGCCCAGA TTCAGTCAGA GTTATTCCCT TCAACTGCTT CAGCAAATGG AAACCTTCAG 
CAATCGCCAG TTTACCAGCA GACTTCTCAC ATGATGAGTG CATTGTCTAC CAATGAGGAT 
ATGCAAATGC AGTGTGAATT GTTTTCTTCT CCTCCTGCAG TTTCTGGAAA TGAAACTTCT 
ACAACTACCA CACAGCAGGT TGCAACCCCT GGCACTACCA TGTTTCAGAC ATCAAGTTCA 
GGAGATGGAG AAGAAACTGG AACACAAGCA AAACAGATTC AGAACAGTGT CTTTCAGACC 
ATGGTCCAAA TGCAACATAG TGGGGACAAT CAACCTCAAG TTAACCTTTT TTCATCCACA 
AAAAGTATGA TGAGTGTTCA GAATAGTGGT ACCCAACAAC AAGGTAATGG TTTATTCCAG 
CAAGGGAATG AGATGATGTC ACTTCAATCT GGAAATTTTT TGCAGCAGTC TTCTCATTCA 
CAGGCCCAAC TTTTTCATCC TCAAAATCCT ATTGCCGATG CTCAGAACCT TTCCCAGGAA 
ACTCAAGGTT CTCTCTTTCA TAGTCCAAAT CCTATTGTCC ACAGTCAGAC TTCTACAACC 
TCCTCTGAAC AAATGCAGCC TCCAATGTTT CACTCTCAAA GTACCATTGC TGTGTTACAG 
GGCTCTTCAG TTCCTCAAGA CCAGCAGTCA ACCAACATAT TTCTTTCCCA GAGTCCCATG 
AATAATCTTC AGACTAACAC AGTAGCCCAA GAAGCATTTT TTGCAGCACC GAACTCAATT 
TCTCCACTTC AGTCAACATC AAACAGTGAA CAACAAGCTG CTTTCCAACA GCAAGCTCCA 
ATATCACACA TCCAGACTCC TATGCTTTCC CAAGAACAGG CACAACCCCC GCAGCAGGGT 
TTATTTCAGC CTCAGGTGGC CCTGGGCTCC CTTCCACCTA ATCCAATGCC TCAAAGCCAA 
CAAGGAACCA TGTTCCAGTC ACAGCACTCA ATAGTTGCCA TGCAGAGTAA CTCTCCATCC 
CAGGAACAGC AGCAGCAGCA GCAACAGCAG CAGCAACAGC AGCAGCAACA ACAACAGAGC 
ATTTTATTCA GTAATCAGAA TACCATGGCT ACAATGGCGT CTCCAAAGCA ACCACCACCA 
AACATGATAT TCAACCCAAA TCAAAATCCA ATGGCTAATC AGGAGCAACA GAACCAGTCA 
ATTTTTCACC AACAAAGTAA CATGGCCCCA ATGAATCAAG AGCAACAGCC CATGCAATTT 
CAGAGTCAGT CCACAGTTTC CTCACTTCAG AACCCAGGTC CTACCCAGTC GGAATCATCA 
CAGACCCCCT TGTTCCATAG CTCTCCTCAG ATTCAGTTGG TACAAGGGTC ACCTAGTTCT 
CAAGAGCAGC AAGTAACTCT CTTCTTATCT CCAGCATCCA TGTCTGCCTT GCAGACCAGT 
ATAAATCAAC AAGATATGCA ACAGTCTCCT CTTTATTCCC CTCAGAACAA CATGCCTGGA 
ATTCAAGGAG CCACATCTTC GCCTCAACCA CAGGCTACTT TATTTCACAA CACAGCAGGA 
GGCACAATGA ACCAACTGCA GAATTCTCCT GGCTCATCTC AGCAGACATC AGGAATGTTC 
TTATTTGGCA TTCAAAATAA CTGTAGTCAG CTTTTAACCT CTGGACCAGC TACATTGCCT 
GATCAGTTGA TGGCCATAAG TCAGCCAGGC CAACCACAAA ACGAGGGCCA GCCACCTGTG 
ACAACACTTC TTTCTCAGCA AATGCCAGAG AATTCTCCAC TGGCATCCTC TATAAACACC 
AACCAGAACA TCGAAAAGAT TGATTTGCTT GTTTCATTGC AAAACCAAGG GAACAACTTG 
ACTGGCTCCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006721188.1
CDS	1203..5657
Translation	MSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_006721125.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006721125.4

ORF Insert Sequence:

ATGAGTCAGA CAAGCGGTGG TGAGGCAGGC TCGCCTCCTC CAGCTGTTGT TGCTGCTGAT 
GCTTCTTCAG CTCCCTCCTC TTCCTCCATG GGCGGTGCTT GCAGCTCCTT TACCACCTCT 
TCCAGCCCTA CCATTTATTC TACCTCAGTC ACCGACAGCA AGGCTATGCA AGTGGAGAGC 
TGCTCCTCAG CCGTGGGGGT AAGTAACAGA GGGGTAAGTG AAAAGCAGTT AACCAGTAAC 
ACAGTTCAGC AGCATCCATC AACACCGAAG AGGCACACAG TCTTGTACAT CTCACCACCA 
CCTGAGGACT TGCTGGATAA CAGTCGGATG TCCTGCCAGG ATGAGGGGTG TGGATTGGAA 
TCTGAGCAGA GCTGCAGTAT GTGGATGGAG GATTCCCCCT CCAACTTCAG TAACATGAGC 
ACCAGTTCCT ACAATGATAA CACTGAGGTA CCTCGTAAAT CACGAAAACG AAATCCAAAG 
CAGAGGCCGG GGGTCAAACG ACGAGATTGT GAAGAATCTA ATATGGATAT ATTTGATGCC 
GACAGTGCCA AAGCACCTCA CTATGTGCTT TCTCAGCTTA CCACGGACAA CAAAGGCAAC 
TCAAAAGCGG GAAATGGAAC ATTGGAAAAC CAAAAAGGAA CTGGAGTAAA GAAGAGCCCT 
ATGTTGTGTG GACAATATCC TGTTAAAAGT GAGGGAAAGG AGCTGAAGAT AGTTGTACAA 
CCTGAGACAC AGCACCGAGC TCGGTACCTG ACTGAGGGCA GCCGTGGCTC AGTGAAAGAT 
AGAACACAGC AAGGCTTTCC TACAGTAAAG CTGGAAGGCC ATAATGAACC TGTAGTGTTG 
CAAGTGTTTG TGGGCAACGA CTCTGGACGA GTGAAACCAC ATGGATTTTA TCAGGCCTGC 
AGAGTAACTG GACGAAATAC AACTCCTTGC AAAGAAGTGG ACATTGAAGG CACTACTGTT 
ATAGAAGTCG GCCTTGATCC TAGCAACAAC ATGACACTGG CGGTGGACTG CGTAGGGATA 
TTGAAATTGA GGAATGCTGA TGTCGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAGAAA 
AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCATGA GGAAAGATGG CTCCACTTTG 
ACACTGCAAA CACCCTCTTC TCCAATTTTG TGTACTCAGC CAGCAGGAGT GCCAGAAATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAAG AAGAAGTGTT TTTAATCGGC 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAGTCAG AAGCTGAAAT TGATATGGAA CTATTTCATC AGAATCATCT TATTGTGAAG 
GTTCCTCCCT ATCATGACCA ACATATAACT TTGCCTGTGT CAGTGGGAAT ATATGTAGTG 
ACAAATGCTG GAAGATCTCA TGATGTTCAA CCATTCACTT ACACTCCAGA CCCAGCAGCA 
GCTGGTGCTT TGAATGTAAA TGTGAAGAAG GAAATATCTA GTCCAGCAAG ACCTTGCTCT 
TTTGAAGAGG CCATGAAAGC AATGAAAACT ACTGGATGTA ATTTAGATAA GGTAAATATT 
ATCCCTAATG CCCTGATGAC TCCACTCATA CCAAGCAGTA TGATTAAGAG TGAAGATGTT 
ACTCCAATGG AAGTAACAGC AGAAAAAAGA TCTTCCACTA TTTTTAAGAC TACAAAGTCT 
GTTGGATCAA CTCAGCAAAC ATTAGAAAAC ATCTCAAACA TAGCAGGAAA TGGCTCTTTT 
TCATCACCAT CATCTTCCCA CCTACCTTCT GAAAATGAAA AACAGCAGCA GATTCAGCCC 
AAGGCATACA ACCCAGAGAC CCTGACAACT ATTCAAACCC AGGACATCTC ACAGCCTGGT 
ACTTTTCCAG CAGTTTCTGC TTCTAGTCAG CTGCCCAACA GCGATGCACT ATTGCAGCAG 
GCTACACAGT TTCAGACAAG AGAAACTCAG TCTAGAGAGA TATTACAGTC AGATGGTACA 
GTGGTTAATT TGTCACAACT GACTGAGGCA TCACAACAAC AGCAGCAGTC ACCACTACAA 
GAACAAGCAC AGACTTTACA GCAGCAGATT TCATCAAATA TTTTTCCATC ACCAAATAGT 
GTGAGTCAGC TTCAGAATAC TATTCAGCAG CTGCAAGCAG GGAGTTTCAC AGGCAGTACT 
GCTAGTGGCA GCAGTGGAAG TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAGCAGCAG 
TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAATGAGA ATGTTCAAGA GCAGCTTAGT 
GCAGATATTT TTCAACAAGT CAGTCAAATT CAGAGTGGTG TAAGCCCTGG AATGTTTTCC 
TCAACAGAGC CAACAGTCCA TACCAGACCA GATAATTTAT TACCTGGAAG AGCTGAAAGT 
GTTCATCCAC AGTCTGAAAA CACGTTATCT AATCAACAGC AGCAGCAGCA GCAGCAACAG 
CAAGTGATGG AATCTTCAGC CGCAATGGTG ATGGAGATGC AACAGAGTAT CTGCCAGGCA 
GCTGCCCAGA TTCAGTCAGA GTTATTCCCT TCAACTGCTT CAGCAAATGG AAACCTTCAG 
CAATCGCCAG TTTACCAGCA GACTTCTCAC ATGATGAGTG CATTGTCTAC CAATGAGGAT 
ATGCAAATGC AGTGTGAATT GTTTTCTTCT CCTCCTGCAG TTTCTGGAAA TGAAACTTCT 
ACAACTACCA CACAGCAGGT TGCAACCCCT GGCACTACCA TGTTTCAGAC ATCAAGTTCA 
GGAGATGGAG AAGAAACTGG AACACAAGCA AAACAGATTC AGAACAGTGT CTTTCAGACC 
ATGGTCCAAA TGCAACATAG TGGGGACAAT CAACCTCAAG TTAACCTTTT TTCATCCACA 
AAAAGTATGA TGAGTGTTCA GAATAGTGGT ACCCAACAAC AAGGTAATGG TTTATTCCAG 
CAAGGGAATG AGATGATGTC ACTTCAATCT GGAAATTTTT TGCAGCAGTC TTCTCATTCA 
CAGGCCCAAC TTTTTCATCC TCAAAATCCT ATTGCCGATG CTCAGAACCT TTCCCAGGAA 
ACTCAAGGTT CTCTCTTTCA TAGTCCAAAT CCTATTGTCC ACAGTCAGAC TTCTACAACC 
TCCTCTGAAC AAATGCAGCC TCCAATGTTT CACTCTCAAA GTACCATTGC TGTGTTACAG 
GGCTCTTCAG TTCCTCAAGA CCAGCAGTCA ACCAACATAT TTCTTTCCCA GAGTCCCATG 
AATAATCTTC AGACTAACAC AGTAGCCCAA GAAGCATTTT TTGCAGCACC GAACTCAATT 
TCTCCACTTC AGTCAACATC AAACAGTGAA CAACAAGCTG CTTTCCAACA GCAAGCTCCA 
ATATCACACA TCCAGACTCC TATGCTTTCC CAAGAACAGG CACAACCCCC GCAGCAGGGT 
TTATTTCAGC CTCAGGTGGC CCTGGGCTCC CTTCCACCTA ATCCAATGCC TCAAAGCCAA 
CAAGGAACCA TGTTCCAGTC ACAGCACTCA ATAGTTGCCA TGCAGAGTAA CTCTCCATCC 
CAGGAACAGC AGCAGCAGCA GCAACAGCAG CAGCAACAGC AGCAGCAACA ACAACAGAGC 
ATTTTATTCA GTAATCAGAA TACCATGGCT ACAATGGCGT CTCCAAAGCA ACCACCACCA 
AACATGATAT TCAACCCAAA TCAAAATCCA ATGGCTAATC AGGAGCAACA GAACCAGTCA 
ATTTTTCACC AACAAAGTAA CATGGCCCCA ATGAATCAAG AGCAACAGCC CATGCAATTT 
CAGAGTCAGT CCACAGTTTC CTCACTTCAG AACCCAGGTC CTACCCAGTC GGAATCATCA 
CAGACCCCCT TGTTCCATAG CTCTCCTCAG ATTCAGTTGG TACAAGGGTC ACCTAGTTCT 
CAAGAGCAGC AAGTAACTCT CTTCTTATCT CCAGCATCCA TGTCTGCCTT GCAGACCAGT 
ATAAATCAAC AAGATATGCA ACAGTCTCCT CTTTATTCCC CTCAGAACAA CATGCCTGGA 
ATTCAAGGAG CCACATCTTC GCCTCAACCA CAGGCTACTT TATTTCACAA CACAGCAGGA 
GGCACAATGA ACCAACTGCA GAATTCTCCT GGCTCATCTC AGCAGACATC AGGAATGTTC 
TTATTTGGCA TTCAAAATAA CTGTAGTCAG CTTTTAACCT CTGGACCAGC TACATTGCCT 
GATCAGTTGA TGGCCATAAG TCAGCCAGGC CAACCACAAA ACGAGGGCCA GCCACCTGTG 
ACAACACTTC TTTCTCAGCA AATGCCAGAG AATTCTCCAC TGGCATCCTC TATAAACACC 
AACCAGAACA TCGAAAAGAT TGATTTGCTT GTTTCATTGC AAAACCAAGG GAACAACTTG 
ACTGGCTCCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_006721125.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006721188.1 Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X2, mRNA.

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CloneID	OHu49247
Clone ID Related Accession (Same CDS sequence)	XM_006721125.4 , XM_006721125.3
Accession Version	XM_006721125.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006721125.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGA CAAGCGGTGG TGAGGCAGGC TCGCCTCCTC CAGCTGTTGT TGCTGCTGAT 
GCTTCTTCAG CTCCCTCCTC TTCCTCCATG GGCGGTGCTT GCAGCTCCTT TACCACCTCT 
TCCAGCCCTA CCATTTATTC TACCTCAGTC ACCGACAGCA AGGCTATGCA AGTGGAGAGC 
TGCTCCTCAG CCGTGGGGGT AAGTAACAGA GGGGTAAGTG AAAAGCAGTT AACCAGTAAC 
ACAGTTCAGC AGCATCCATC AACACCGAAG AGGCACACAG TCTTGTACAT CTCACCACCA 
CCTGAGGACT TGCTGGATAA CAGTCGGATG TCCTGCCAGG ATGAGGGGTG TGGATTGGAA 
TCTGAGCAGA GCTGCAGTAT GTGGATGGAG GATTCCCCCT CCAACTTCAG TAACATGAGC 
ACCAGTTCCT ACAATGATAA CACTGAGGTA CCTCGTAAAT CACGAAAACG AAATCCAAAG 
CAGAGGCCGG GGGTCAAACG ACGAGATTGT GAAGAATCTA ATATGGATAT ATTTGATGCC 
GACAGTGCCA AAGCACCTCA CTATGTGCTT TCTCAGCTTA CCACGGACAA CAAAGGCAAC 
TCAAAAGCGG GAAATGGAAC ATTGGAAAAC CAAAAAGGAA CTGGAGTAAA GAAGAGCCCT 
ATGTTGTGTG GACAATATCC TGTTAAAAGT GAGGGAAAGG AGCTGAAGAT AGTTGTACAA 
CCTGAGACAC AGCACCGAGC TCGGTACCTG ACTGAGGGCA GCCGTGGCTC AGTGAAAGAT 
AGAACACAGC AAGGCTTTCC TACAGTAAAG CTGGAAGGCC ATAATGAACC TGTAGTGTTG 
CAAGTGTTTG TGGGCAACGA CTCTGGACGA GTGAAACCAC ATGGATTTTA TCAGGCCTGC 
AGAGTAACTG GACGAAATAC AACTCCTTGC AAAGAAGTGG ACATTGAAGG CACTACTGTT 
ATAGAAGTCG GCCTTGATCC TAGCAACAAC ATGACACTGG CGGTGGACTG CGTAGGGATA 
TTGAAATTGA GGAATGCTGA TGTCGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAGAAA 
AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCATGA GGAAAGATGG CTCCACTTTG 
ACACTGCAAA CACCCTCTTC TCCAATTTTG TGTACTCAGC CAGCAGGAGT GCCAGAAATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAAG AAGAAGTGTT TTTAATCGGC 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAGTCAG AAGCTGAAAT TGATATGGAA CTATTTCATC AGAATCATCT TATTGTGAAG 
GTTCCTCCCT ATCATGACCA ACATATAACT TTGCCTGTGT CAGTGGGAAT ATATGTAGTG 
ACAAATGCTG GAAGATCTCA TGATGTTCAA CCATTCACTT ACACTCCAGA CCCAGCAGCA 
GCTGGTGCTT TGAATGTAAA TGTGAAGAAG GAAATATCTA GTCCAGCAAG ACCTTGCTCT 
TTTGAAGAGG CCATGAAAGC AATGAAAACT ACTGGATGTA ATTTAGATAA GGTAAATATT 
ATCCCTAATG CCCTGATGAC TCCACTCATA CCAAGCAGTA TGATTAAGAG TGAAGATGTT 
ACTCCAATGG AAGTAACAGC AGAAAAAAGA TCTTCCACTA TTTTTAAGAC TACAAAGTCT 
GTTGGATCAA CTCAGCAAAC ATTAGAAAAC ATCTCAAACA TAGCAGGAAA TGGCTCTTTT 
TCATCACCAT CATCTTCCCA CCTACCTTCT GAAAATGAAA AACAGCAGCA GATTCAGCCC 
AAGGCATACA ACCCAGAGAC CCTGACAACT ATTCAAACCC AGGACATCTC ACAGCCTGGT 
ACTTTTCCAG CAGTTTCTGC TTCTAGTCAG CTGCCCAACA GCGATGCACT ATTGCAGCAG 
GCTACACAGT TTCAGACAAG AGAAACTCAG TCTAGAGAGA TATTACAGTC AGATGGTACA 
GTGGTTAATT TGTCACAACT GACTGAGGCA TCACAACAAC AGCAGCAGTC ACCACTACAA 
GAACAAGCAC AGACTTTACA GCAGCAGATT TCATCAAATA TTTTTCCATC ACCAAATAGT 
GTGAGTCAGC TTCAGAATAC TATTCAGCAG CTGCAAGCAG GGAGTTTCAC AGGCAGTACT 
GCTAGTGGCA GCAGTGGAAG TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAGCAGCAG 
TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAATGAGA ATGTTCAAGA GCAGCTTAGT 
GCAGATATTT TTCAACAAGT CAGTCAAATT CAGAGTGGTG TAAGCCCTGG AATGTTTTCC 
TCAACAGAGC CAACAGTCCA TACCAGACCA GATAATTTAT TACCTGGAAG AGCTGAAAGT 
GTTCATCCAC AGTCTGAAAA CACGTTATCT AATCAACAGC AGCAGCAGCA GCAGCAACAG 
CAAGTGATGG AATCTTCAGC CGCAATGGTG ATGGAGATGC AACAGAGTAT CTGCCAGGCA 
GCTGCCCAGA TTCAGTCAGA GTTATTCCCT TCAACTGCTT CAGCAAATGG AAACCTTCAG 
CAATCGCCAG TTTACCAGCA GACTTCTCAC ATGATGAGTG CATTGTCTAC CAATGAGGAT 
ATGCAAATGC AGTGTGAATT GTTTTCTTCT CCTCCTGCAG TTTCTGGAAA TGAAACTTCT 
ACAACTACCA CACAGCAGGT TGCAACCCCT GGCACTACCA TGTTTCAGAC ATCAAGTTCA 
GGAGATGGAG AAGAAACTGG AACACAAGCA AAACAGATTC AGAACAGTGT CTTTCAGACC 
ATGGTCCAAA TGCAACATAG TGGGGACAAT CAACCTCAAG TTAACCTTTT TTCATCCACA 
AAAAGTATGA TGAGTGTTCA GAATAGTGGT ACCCAACAAC AAGGTAATGG TTTATTCCAG 
CAAGGGAATG AGATGATGTC ACTTCAATCT GGAAATTTTT TGCAGCAGTC TTCTCATTCA 
CAGGCCCAAC TTTTTCATCC TCAAAATCCT ATTGCCGATG CTCAGAACCT TTCCCAGGAA 
ACTCAAGGTT CTCTCTTTCA TAGTCCAAAT CCTATTGTCC ACAGTCAGAC TTCTACAACC 
TCCTCTGAAC AAATGCAGCC TCCAATGTTT CACTCTCAAA GTACCATTGC TGTGTTACAG 
GGCTCTTCAG TTCCTCAAGA CCAGCAGTCA ACCAACATAT TTCTTTCCCA GAGTCCCATG 
AATAATCTTC AGACTAACAC AGTAGCCCAA GAAGCATTTT TTGCAGCACC GAACTCAATT 
TCTCCACTTC AGTCAACATC AAACAGTGAA CAACAAGCTG CTTTCCAACA GCAAGCTCCA 
ATATCACACA TCCAGACTCC TATGCTTTCC CAAGAACAGG CACAACCCCC GCAGCAGGGT 
TTATTTCAGC CTCAGGTGGC CCTGGGCTCC CTTCCACCTA ATCCAATGCC TCAAAGCCAA 
CAAGGAACCA TGTTCCAGTC ACAGCACTCA ATAGTTGCCA TGCAGAGTAA CTCTCCATCC 
CAGGAACAGC AGCAGCAGCA GCAACAGCAG CAGCAACAGC AGCAGCAACA ACAACAGAGC 
ATTTTATTCA GTAATCAGAA TACCATGGCT ACAATGGCGT CTCCAAAGCA ACCACCACCA 
AACATGATAT TCAACCCAAA TCAAAATCCA ATGGCTAATC AGGAGCAACA GAACCAGTCA 
ATTTTTCACC AACAAAGTAA CATGGCCCCA ATGAATCAAG AGCAACAGCC CATGCAATTT 
CAGAGTCAGT CCACAGTTTC CTCACTTCAG AACCCAGGTC CTACCCAGTC GGAATCATCA 
CAGACCCCCT TGTTCCATAG CTCTCCTCAG ATTCAGTTGG TACAAGGGTC ACCTAGTTCT 
CAAGAGCAGC AAGTAACTCT CTTCTTATCT CCAGCATCCA TGTCTGCCTT GCAGACCAGT 
ATAAATCAAC AAGATATGCA ACAGTCTCCT CTTTATTCCC CTCAGAACAA CATGCCTGGA 
ATTCAAGGAG CCACATCTTC GCCTCAACCA CAGGCTACTT TATTTCACAA CACAGCAGGA 
GGCACAATGA ACCAACTGCA GAATTCTCCT GGCTCATCTC AGCAGACATC AGGAATGTTC 
TTATTTGGCA TTCAAAATAA CTGTAGTCAG CTTTTAACCT CTGGACCAGC TACATTGCCT 
GATCAGTTGA TGGCCATAAG TCAGCCAGGC CAACCACAAA ACGAGGGCCA GCCACCTGTG 
ACAACACTTC TTTCTCAGCA AATGCCAGAG AATTCTCCAC TGGCATCCTC TATAAACACC 
AACCAGAACA TCGAAAAGAT TGATTTGCTT GTTTCATTGC AAAACCAAGG GAACAACTTG 
ACTGGCTCCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006721188.1
CDS	1203..5657
Translation	MSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_006721125.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006721125.3

ORF Insert Sequence:

ATGAGTCAGA CAAGCGGTGG TGAGGCAGGC TCGCCTCCTC CAGCTGTTGT TGCTGCTGAT 
GCTTCTTCAG CTCCCTCCTC TTCCTCCATG GGCGGTGCTT GCAGCTCCTT TACCACCTCT 
TCCAGCCCTA CCATTTATTC TACCTCAGTC ACCGACAGCA AGGCTATGCA AGTGGAGAGC 
TGCTCCTCAG CCGTGGGGGT AAGTAACAGA GGGGTAAGTG AAAAGCAGTT AACCAGTAAC 
ACAGTTCAGC AGCATCCATC AACACCGAAG AGGCACACAG TCTTGTACAT CTCACCACCA 
CCTGAGGACT TGCTGGATAA CAGTCGGATG TCCTGCCAGG ATGAGGGGTG TGGATTGGAA 
TCTGAGCAGA GCTGCAGTAT GTGGATGGAG GATTCCCCCT CCAACTTCAG TAACATGAGC 
ACCAGTTCCT ACAATGATAA CACTGAGGTA CCTCGTAAAT CACGAAAACG AAATCCAAAG 
CAGAGGCCGG GGGTCAAACG ACGAGATTGT GAAGAATCTA ATATGGATAT ATTTGATGCC 
GACAGTGCCA AAGCACCTCA CTATGTGCTT TCTCAGCTTA CCACGGACAA CAAAGGCAAC 
TCAAAAGCGG GAAATGGAAC ATTGGAAAAC CAAAAAGGAA CTGGAGTAAA GAAGAGCCCT 
ATGTTGTGTG GACAATATCC TGTTAAAAGT GAGGGAAAGG AGCTGAAGAT AGTTGTACAA 
CCTGAGACAC AGCACCGAGC TCGGTACCTG ACTGAGGGCA GCCGTGGCTC AGTGAAAGAT 
AGAACACAGC AAGGCTTTCC TACAGTAAAG CTGGAAGGCC ATAATGAACC TGTAGTGTTG 
CAAGTGTTTG TGGGCAACGA CTCTGGACGA GTGAAACCAC ATGGATTTTA TCAGGCCTGC 
AGAGTAACTG GACGAAATAC AACTCCTTGC AAAGAAGTGG ACATTGAAGG CACTACTGTT 
ATAGAAGTCG GCCTTGATCC TAGCAACAAC ATGACACTGG CGGTGGACTG CGTAGGGATA 
TTGAAATTGA GGAATGCTGA TGTCGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAGAAA 
AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCATGA GGAAAGATGG CTCCACTTTG 
ACACTGCAAA CACCCTCTTC TCCAATTTTG TGTACTCAGC CAGCAGGAGT GCCAGAAATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAAG AAGAAGTGTT TTTAATCGGC 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAGTCAG AAGCTGAAAT TGATATGGAA CTATTTCATC AGAATCATCT TATTGTGAAG 
GTTCCTCCCT ATCATGACCA ACATATAACT TTGCCTGTGT CAGTGGGAAT ATATGTAGTG 
ACAAATGCTG GAAGATCTCA TGATGTTCAA CCATTCACTT ACACTCCAGA CCCAGCAGCA 
GCTGGTGCTT TGAATGTAAA TGTGAAGAAG GAAATATCTA GTCCAGCAAG ACCTTGCTCT 
TTTGAAGAGG CCATGAAAGC AATGAAAACT ACTGGATGTA ATTTAGATAA GGTAAATATT 
ATCCCTAATG CCCTGATGAC TCCACTCATA CCAAGCAGTA TGATTAAGAG TGAAGATGTT 
ACTCCAATGG AAGTAACAGC AGAAAAAAGA TCTTCCACTA TTTTTAAGAC TACAAAGTCT 
GTTGGATCAA CTCAGCAAAC ATTAGAAAAC ATCTCAAACA TAGCAGGAAA TGGCTCTTTT 
TCATCACCAT CATCTTCCCA CCTACCTTCT GAAAATGAAA AACAGCAGCA GATTCAGCCC 
AAGGCATACA ACCCAGAGAC CCTGACAACT ATTCAAACCC AGGACATCTC ACAGCCTGGT 
ACTTTTCCAG CAGTTTCTGC TTCTAGTCAG CTGCCCAACA GCGATGCACT ATTGCAGCAG 
GCTACACAGT TTCAGACAAG AGAAACTCAG TCTAGAGAGA TATTACAGTC AGATGGTACA 
GTGGTTAATT TGTCACAACT GACTGAGGCA TCACAACAAC AGCAGCAGTC ACCACTACAA 
GAACAAGCAC AGACTTTACA GCAGCAGATT TCATCAAATA TTTTTCCATC ACCAAATAGT 
GTGAGTCAGC TTCAGAATAC TATTCAGCAG CTGCAAGCAG GGAGTTTCAC AGGCAGTACT 
GCTAGTGGCA GCAGTGGAAG TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAGCAGCAG 
TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAATGAGA ATGTTCAAGA GCAGCTTAGT 
GCAGATATTT TTCAACAAGT CAGTCAAATT CAGAGTGGTG TAAGCCCTGG AATGTTTTCC 
TCAACAGAGC CAACAGTCCA TACCAGACCA GATAATTTAT TACCTGGAAG AGCTGAAAGT 
GTTCATCCAC AGTCTGAAAA CACGTTATCT AATCAACAGC AGCAGCAGCA GCAGCAACAG 
CAAGTGATGG AATCTTCAGC CGCAATGGTG ATGGAGATGC AACAGAGTAT CTGCCAGGCA 
GCTGCCCAGA TTCAGTCAGA GTTATTCCCT TCAACTGCTT CAGCAAATGG AAACCTTCAG 
CAATCGCCAG TTTACCAGCA GACTTCTCAC ATGATGAGTG CATTGTCTAC CAATGAGGAT 
ATGCAAATGC AGTGTGAATT GTTTTCTTCT CCTCCTGCAG TTTCTGGAAA TGAAACTTCT 
ACAACTACCA CACAGCAGGT TGCAACCCCT GGCACTACCA TGTTTCAGAC ATCAAGTTCA 
GGAGATGGAG AAGAAACTGG AACACAAGCA AAACAGATTC AGAACAGTGT CTTTCAGACC 
ATGGTCCAAA TGCAACATAG TGGGGACAAT CAACCTCAAG TTAACCTTTT TTCATCCACA 
AAAAGTATGA TGAGTGTTCA GAATAGTGGT ACCCAACAAC AAGGTAATGG TTTATTCCAG 
CAAGGGAATG AGATGATGTC ACTTCAATCT GGAAATTTTT TGCAGCAGTC TTCTCATTCA 
CAGGCCCAAC TTTTTCATCC TCAAAATCCT ATTGCCGATG CTCAGAACCT TTCCCAGGAA 
ACTCAAGGTT CTCTCTTTCA TAGTCCAAAT CCTATTGTCC ACAGTCAGAC TTCTACAACC 
TCCTCTGAAC AAATGCAGCC TCCAATGTTT CACTCTCAAA GTACCATTGC TGTGTTACAG 
GGCTCTTCAG TTCCTCAAGA CCAGCAGTCA ACCAACATAT TTCTTTCCCA GAGTCCCATG 
AATAATCTTC AGACTAACAC AGTAGCCCAA GAAGCATTTT TTGCAGCACC GAACTCAATT 
TCTCCACTTC AGTCAACATC AAACAGTGAA CAACAAGCTG CTTTCCAACA GCAAGCTCCA 
ATATCACACA TCCAGACTCC TATGCTTTCC CAAGAACAGG CACAACCCCC GCAGCAGGGT 
TTATTTCAGC CTCAGGTGGC CCTGGGCTCC CTTCCACCTA ATCCAATGCC TCAAAGCCAA 
CAAGGAACCA TGTTCCAGTC ACAGCACTCA ATAGTTGCCA TGCAGAGTAA CTCTCCATCC 
CAGGAACAGC AGCAGCAGCA GCAACAGCAG CAGCAACAGC AGCAGCAACA ACAACAGAGC 
ATTTTATTCA GTAATCAGAA TACCATGGCT ACAATGGCGT CTCCAAAGCA ACCACCACCA 
AACATGATAT TCAACCCAAA TCAAAATCCA ATGGCTAATC AGGAGCAACA GAACCAGTCA 
ATTTTTCACC AACAAAGTAA CATGGCCCCA ATGAATCAAG AGCAACAGCC CATGCAATTT 
CAGAGTCAGT CCACAGTTTC CTCACTTCAG AACCCAGGTC CTACCCAGTC GGAATCATCA 
CAGACCCCCT TGTTCCATAG CTCTCCTCAG ATTCAGTTGG TACAAGGGTC ACCTAGTTCT 
CAAGAGCAGC AAGTAACTCT CTTCTTATCT CCAGCATCCA TGTCTGCCTT GCAGACCAGT 
ATAAATCAAC AAGATATGCA ACAGTCTCCT CTTTATTCCC CTCAGAACAA CATGCCTGGA 
ATTCAAGGAG CCACATCTTC GCCTCAACCA CAGGCTACTT TATTTCACAA CACAGCAGGA 
GGCACAATGA ACCAACTGCA GAATTCTCCT GGCTCATCTC AGCAGACATC AGGAATGTTC 
TTATTTGGCA TTCAAAATAA CTGTAGTCAG CTTTTAACCT CTGGACCAGC TACATTGCCT 
GATCAGTTGA TGGCCATAAG TCAGCCAGGC CAACCACAAA ACGAGGGCCA GCCACCTGTG 
ACAACACTTC TTTCTCAGCA AATGCCAGAG AATTCTCCAC TGGCATCCTC TATAAACACC 
AACCAGAACA TCGAAAAGAT TGATTTGCTT GTTTCATTGC AAAACCAAGG GAACAACTTG 
ACTGGCTCCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_011522817.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521119.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu64333
Clone ID Related Accession (Same CDS sequence)	XM_011522817.3 , XM_011522817.2
Accession Version	XM_011522817.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011522817.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTCTACTCGC GAGAATCTGT CTATGATCTT CTCCCAAAGG AGTTACAGTT ACCTCCATCT 
AGAGAAACAT CTGTAGCATC AATGAGTCAG ACAAGCGGTG GTGAGGCAGG CTCGCCTCCT 
CCAGCTGTTG TTGCTGCTGA TGCTTCTTCA GCTCCCTCCT CTTCCTCCAT GGGCGGTGCT 
TGCAGCTCCT TTACCACCTC TTCCAGCCCT ACCATTTATT CTACCTCAGT CACCGACAGC 
AAGGCTATGC AAGTGGAGAG CTGCTCCTCA GCCGTGGGGG TAAGTAACAG AGGGGTAAGT 
GAAAAGCAGT TAACCAGTAA CACAGTTCAG CAGCATCCAT CAACACCGAA GAGGCACACA 
GTCTTGTACA TCTCACCACC ACCTGAGGAC TTGCTGGATA ACAGTCGGAT GTCCTGCCAG 
GATGAGGGGT GTGGATTGGA ATCTGAGCAG AGCTGCAGTA TGTGGATGGA GGATTCCCCC 
TCCAACTTCA GTAACATGAG CACCAGTTCC TACAATGATA ACACTGAGGT ACCTCGTAAA 
TCACGAAAAC GAAATCCAAA GCAGAGGCCG GGGGTCAAAC GACGAGATTG TGAAGAATCT 
AATATGGATA TATTTGATGC CGACAGTGCC AAAGCACCTC ACTATGTGCT TTCTCAGCTT 
ACCACGGACA ACAAAGGCAA CTCAAAAGCG GGAAATGGAA CATTGGAAAA CCAAAAAGGA 
ACTGGAGTAA AGAAGAGCCC TATGTTGTGT GGACAATATC CTGTTAAAAG TGAGGGAAAG 
GAGCTGAAGA TAGTTGTACA ACCTGAGACA CAGCACCGAG CTCGGTACCT GACTGAGGGC 
AGCCGTGGCT CAGTGAAAGA TAGAACACAG CAAGGCTTTC CTACAGTAAA GCTGGAAGGC 
CATAATGAAC CTGTAGTGTT GCAAGTGTTT GTGGGCAACG ACTCTGGACG AGTGAAACCA 
CATGGATTTT ATCAGGCCTG CAGAGTAACT GGACGAAATA CAACTCCTTG CAAAGAAGTG 
GACATTGAAG GCACTACTGT TATAGAAGTC GGCCTTGATC CTAGCAACAA CATGACACTG 
GCGGTGGACT GCGTAGGGAT ATTGAAATTG AGGAATGCTG ATGTCGAAGC CAGAATAGGA 
ATTGCTGGTT CCAAGAAGAA AAGCACTCGT GCCAGATTGG TTTTTCGAGT TAATATCATG 
AGGAAAGATG GCTCCACTTT GACACTGCAA ACACCCTCTT CTCCAATTTT GTGTACTCAG 
CCAGCAGGAG TGCCAGAAAT CTTAAAGAAA AGCTTGCATA GCTGTTCAGT GAAAGGAGAA 
GAAGAAGTGT TTTTAATCGG CAAGAACTTT CTGAAAGGAA CTAAAGTTAT TTTCCAAGAA 
AATGTTTCTG ATGAAAACTC TTGGAAGTCA GAAGCTGAAA TTGATATGGA ACTATTTCAT 
CAGAATCATC TTATTGTGAA GGTTCCTCCC TATCATGACC AACATATAAC TTTGCCTGTG 
TCAGTGGGAA TATATGTAGT GACAAATGCT GGAAGATCTC ATGATGTTCA ACCATTCACT 
TACACTCCAG ACCCAGCAGC TGGTGCTTTG AATGTAAATG TGAAGAAGGA AATATCTAGT 
CCAGCAAGAC CTTGCTCTTT TGAAGAGGCC ATGAAAGCAA TGAAAACTAC TGGATGTAAT 
TTAGATAAGG TAAATATTAT CCCTAATGCC CTGATGACTC CACTCATACC AAGCAGTATG 
ATTAAGAGTG AAGATGTTAC TCCAATGGAA GTAACAGCAG AAAAAAGATC TTCCACTATT 
TTTAAGACTA CAAAGTCTGT TGGATCAACT CAGCAAACAT TAGAAAACAT CTCAAACATA 
GCAGGAAATG GCTCTTTTTC ATCACCATCA TCTTCCCACC TACCTTCTGA AAATGAAAAA 
CAGCAGCAGA TTCAGCCCAA GGCATACAAC CCAGAGACCC TGACAACTAT TCAAACCCAG 
GACATCTCAC AGCCTGGTAC TTTTCCAGCA GTTTCTGCTT CTAGTCAGCT GCCCAACAGC 
GATGCACTAT TGCAGCAGGC TACACAGTTT CAGACAAGAG AAACTCAGTC TAGAGAGATA 
TTACAGTCAG ATGGTACAGT GGTTAATTTG TCACAACTGA CTGAGGCATC ACAACAACAG 
CAGCAGTCAC CACTACAAGA ACAAGCACAG ACTTTACAGC AGCAGATTTC ATCAAATATT 
TTTCCATCAC CAAATAGTGT GAGTCAGCTT CAGAATACTA TTCAGCAGCT GCAAGCAGGG 
AGTTTCACAG GCAGTACTGC TAGTGGCAGC AGTGGAAGTG TTGACTTGGT CCAACAAGTT 
TTAGAGGCAC AGCAGCAGTT ATCTTCAGTT TTATTTTCTG CTCCAGATGG TAATGAGAAT 
GTTCAAGAGC AGCTTAGTGC AGATATTTTT CAACAAGTCA GTCAAATTCA GAGTGGTGTA 
AGCCCTGGAA TGTTTTCCTC AACAGAGCCA ACAGTCCATA CCAGACCAGA TAATTTATTA 
CCTGGAAGAG CTGAAAGTGT TCATCCACAG TCTGAAAACA CGTTATCTAA TCAACAGCAG 
CAGCAGCAGC AGCAACAGCA AGTGATGGAA TCTTCAGCCG CAATGGTGAT GGAGATGCAA 
CAGAGTATCT GCCAGGCAGC TGCCCAGATT CAGTCAGAGT TATTCCCTTC AACTGCTTCA 
GCAAATGGAA ACCTTCAGCA ATCGCCAGTT TACCAGCAGA CTTCTCACAT GATGAGTGCA 
TTGTCTACCA ATGAGGATAT GCAAATGCAG TGTGAATTGT TTTCTTCTCC TCCTGCAGTT 
TCTGGAAATG AAACTTCTAC AACTACCACA CAGCAGGTTG CAACCCCTGG CACTACCATG 
TTTCAGACAT CAAGTTCAGG AGATGGAGAA GAAACTGGAA CACAAGCAAA ACAGATTCAG 
AACAGTGTCT TTCAGACCAT GGTCCAAATG CAACATAGTG GGGACAATCA ACCTCAAGTT 
AACCTTTTTT CATCCACAAA AAGTATGATG AGTGTTCAGA ATAGTGGTAC CCAACAACAA 
GGTAATGGTT TATTCCAGCA AGGGAATGAG ATGATGTCAC TTCAATCTGG AAATTTTTTG 
CAGCAGTCTT CTCATTCACA GGCCCAACTT TTTCATCCTC AAAATCCTAT TGCCGATGCT 
CAGAACCTTT CCCAGGAAAC TCAAGGTTCT CTCTTTCATA GTCCAAATCC TATTGTCCAC 
AGTCAGACTT CTACAACCTC CTCTGAACAA ATGCAGCCTC CAATGTTTCA CTCTCAAAGT 
ACCATTGCTG TGTTACAGGG CTCTTCAGTT CCTCAAGACC AGCAGTCAAC CAACATATTT 
CTTTCCCAGA GTCCCATGAA TAATCTTCAG ACTAACACAG TAGCCCAAGA AGCATTTTTT 
GCAGCACCGA ACTCAATTTC TCCACTTCAG TCAACATCAA ACAGTGAACA ACAAGCTGCT 
TTCCAACAGC AAGCTCCAAT ATCACACATC CAGACTCCTA TGCTTTCCCA AGAACAGGCA 
CAACCCCCGC AGCAGGGTTT ATTTCAGCCT CAGGTGGCCC TGGGCTCCCT TCCACCTAAT 
CCAATGCCTC AAAGCCAACA AGGAACCATG TTCCAGTCAC AGCACTCAAT AGTTGCCATG 
CAGAGTAACT CTCCATCCCA GGAACAGCAG CAGCAGCAGC AACAGCAGCA GCAACAGCAG 
CAGCAACAAC AACAGAGCAT TTTATTCAGT AATCAGAATA CCATGGCTAC AATGGCGTCT 
CCAAAGCAAC CACCACCAAA CATGATATTC AACCCAAATC AAAATCCAAT GGCTAATCAG 
GAGCAACAGA ACCAGTCAAT TTTTCACCAA CAAAGTAACA TGGCCCCAAT GAATCAAGAG 
CAACAGCCCA TGCAATTTCA GAGTCAGTCC ACAGTTTCCT CACTTCAGAA CCCAGGTCCT 
ACCCAGTCGG AATCATCACA GACCCCCTTG TTCCATAGCT CTCCTCAGAT TCAGTTGGTA 
CAAGGGTCAC CTAGTTCTCA AGAGCAGCAA GTAACTCTCT TCTTATCTCC AGCATCCATG 
TCTGCCTTGC AGACCAGTAT AAATCAACAA GATATGCAAC AGTCTCCTCT TTATTCCCCT 
CAGAACAACA TGCCTGGAAT TCAAGGAGCC ACATCTTCGC CTCAACCACA GGCTACTTTA 
TTTCACAACA CAGCAGGAGG CACAATGAAC CAACTGCAGA ATTCTCCTGG CTCATCTCAG 
CAGACATCAG GAATGTTCTT ATTTGGCATT CAAAATAACT GTAGTCAGCT TTTAACCTCT 
GGACCAGCTA CATTGCCTGA TCAGTTGATG GCCATAAGTC AGCCAGGCCA ACCACAAAAC 
GAGGGCCAGC CACCTGTGAC AACACTTCTT TCTCAGCAAA TGCCAGAGAA TTCTCCACTG 
GCATCCTCTA TAAACACCAA CCAGAACATC GAAAAGATTG ATTTGCTTGT TTCATTGCAA 
AACCAAGGGA ACAACTTGAC TGGCTCCTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521119.1
CDS	337..4929
Translation	MPSDFISLLSADLDLESPKSLYSRESVYDLLPKELQLPPSRETSVASMSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_011522817.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522817.3

ORF Insert Sequence:

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTCTACTCGC GAGAATCTGT CTATGATCTT CTCCCAAAGG AGTTACAGTT ACCTCCATCT 
AGAGAAACAT CTGTAGCATC AATGAGTCAG ACAAGCGGTG GTGAGGCAGG CTCGCCTCCT 
CCAGCTGTTG TTGCTGCTGA TGCTTCTTCA GCTCCCTCCT CTTCCTCCAT GGGCGGTGCT 
TGCAGCTCCT TTACCACCTC TTCCAGCCCT ACCATTTATT CTACCTCAGT CACCGACAGC 
AAGGCTATGC AAGTGGAGAG CTGCTCCTCA GCCGTGGGGG TAAGTAACAG AGGGGTAAGT 
GAAAAGCAGT TAACCAGTAA CACAGTTCAG CAGCATCCAT CAACACCGAA GAGGCACACA 
GTCTTGTACA TCTCACCACC ACCTGAGGAC TTGCTGGATA ACAGTCGGAT GTCCTGCCAG 
GATGAGGGGT GTGGATTGGA ATCTGAGCAG AGCTGCAGTA TGTGGATGGA GGATTCCCCC 
TCCAACTTCA GTAACATGAG CACCAGTTCC TACAATGATA ACACTGAGGT ACCTCGTAAA 
TCACGAAAAC GAAATCCAAA GCAGAGGCCG GGGGTCAAAC GACGAGATTG TGAAGAATCT 
AATATGGATA TATTTGATGC CGACAGTGCC AAAGCACCTC ACTATGTGCT TTCTCAGCTT 
ACCACGGACA ACAAAGGCAA CTCAAAAGCG GGAAATGGAA CATTGGAAAA CCAAAAAGGA 
ACTGGAGTAA AGAAGAGCCC TATGTTGTGT GGACAATATC CTGTTAAAAG TGAGGGAAAG 
GAGCTGAAGA TAGTTGTACA ACCTGAGACA CAGCACCGAG CTCGGTACCT GACTGAGGGC 
AGCCGTGGCT CAGTGAAAGA TAGAACACAG CAAGGCTTTC CTACAGTAAA GCTGGAAGGC 
CATAATGAAC CTGTAGTGTT GCAAGTGTTT GTGGGCAACG ACTCTGGACG AGTGAAACCA 
CATGGATTTT ATCAGGCCTG CAGAGTAACT GGACGAAATA CAACTCCTTG CAAAGAAGTG 
GACATTGAAG GCACTACTGT TATAGAAGTC GGCCTTGATC CTAGCAACAA CATGACACTG 
GCGGTGGACT GCGTAGGGAT ATTGAAATTG AGGAATGCTG ATGTCGAAGC CAGAATAGGA 
ATTGCTGGTT CCAAGAAGAA AAGCACTCGT GCCAGATTGG TTTTTCGAGT TAATATCATG 
AGGAAAGATG GCTCCACTTT GACACTGCAA ACACCCTCTT CTCCAATTTT GTGTACTCAG 
CCAGCAGGAG TGCCAGAAAT CTTAAAGAAA AGCTTGCATA GCTGTTCAGT GAAAGGAGAA 
GAAGAAGTGT TTTTAATCGG CAAGAACTTT CTGAAAGGAA CTAAAGTTAT TTTCCAAGAA 
AATGTTTCTG ATGAAAACTC TTGGAAGTCA GAAGCTGAAA TTGATATGGA ACTATTTCAT 
CAGAATCATC TTATTGTGAA GGTTCCTCCC TATCATGACC AACATATAAC TTTGCCTGTG 
TCAGTGGGAA TATATGTAGT GACAAATGCT GGAAGATCTC ATGATGTTCA ACCATTCACT 
TACACTCCAG ACCCAGCAGC TGGTGCTTTG AATGTAAATG TGAAGAAGGA AATATCTAGT 
CCAGCAAGAC CTTGCTCTTT TGAAGAGGCC ATGAAAGCAA TGAAAACTAC TGGATGTAAT 
TTAGATAAGG TAAATATTAT CCCTAATGCC CTGATGACTC CACTCATACC AAGCAGTATG 
ATTAAGAGTG AAGATGTTAC TCCAATGGAA GTAACAGCAG AAAAAAGATC TTCCACTATT 
TTTAAGACTA CAAAGTCTGT TGGATCAACT CAGCAAACAT TAGAAAACAT CTCAAACATA 
GCAGGAAATG GCTCTTTTTC ATCACCATCA TCTTCCCACC TACCTTCTGA AAATGAAAAA 
CAGCAGCAGA TTCAGCCCAA GGCATACAAC CCAGAGACCC TGACAACTAT TCAAACCCAG 
GACATCTCAC AGCCTGGTAC TTTTCCAGCA GTTTCTGCTT CTAGTCAGCT GCCCAACAGC 
GATGCACTAT TGCAGCAGGC TACACAGTTT CAGACAAGAG AAACTCAGTC TAGAGAGATA 
TTACAGTCAG ATGGTACAGT GGTTAATTTG TCACAACTGA CTGAGGCATC ACAACAACAG 
CAGCAGTCAC CACTACAAGA ACAAGCACAG ACTTTACAGC AGCAGATTTC ATCAAATATT 
TTTCCATCAC CAAATAGTGT GAGTCAGCTT CAGAATACTA TTCAGCAGCT GCAAGCAGGG 
AGTTTCACAG GCAGTACTGC TAGTGGCAGC AGTGGAAGTG TTGACTTGGT CCAACAAGTT 
TTAGAGGCAC AGCAGCAGTT ATCTTCAGTT TTATTTTCTG CTCCAGATGG TAATGAGAAT 
GTTCAAGAGC AGCTTAGTGC AGATATTTTT CAACAAGTCA GTCAAATTCA GAGTGGTGTA 
AGCCCTGGAA TGTTTTCCTC AACAGAGCCA ACAGTCCATA CCAGACCAGA TAATTTATTA 
CCTGGAAGAG CTGAAAGTGT TCATCCACAG TCTGAAAACA CGTTATCTAA TCAACAGCAG 
CAGCAGCAGC AGCAACAGCA AGTGATGGAA TCTTCAGCCG CAATGGTGAT GGAGATGCAA 
CAGAGTATCT GCCAGGCAGC TGCCCAGATT CAGTCAGAGT TATTCCCTTC AACTGCTTCA 
GCAAATGGAA ACCTTCAGCA ATCGCCAGTT TACCAGCAGA CTTCTCACAT GATGAGTGCA 
TTGTCTACCA ATGAGGATAT GCAAATGCAG TGTGAATTGT TTTCTTCTCC TCCTGCAGTT 
TCTGGAAATG AAACTTCTAC AACTACCACA CAGCAGGTTG CAACCCCTGG CACTACCATG 
TTTCAGACAT CAAGTTCAGG AGATGGAGAA GAAACTGGAA CACAAGCAAA ACAGATTCAG 
AACAGTGTCT TTCAGACCAT GGTCCAAATG CAACATAGTG GGGACAATCA ACCTCAAGTT 
AACCTTTTTT CATCCACAAA AAGTATGATG AGTGTTCAGA ATAGTGGTAC CCAACAACAA 
GGTAATGGTT TATTCCAGCA AGGGAATGAG ATGATGTCAC TTCAATCTGG AAATTTTTTG 
CAGCAGTCTT CTCATTCACA GGCCCAACTT TTTCATCCTC AAAATCCTAT TGCCGATGCT 
CAGAACCTTT CCCAGGAAAC TCAAGGTTCT CTCTTTCATA GTCCAAATCC TATTGTCCAC 
AGTCAGACTT CTACAACCTC CTCTGAACAA ATGCAGCCTC CAATGTTTCA CTCTCAAAGT 
ACCATTGCTG TGTTACAGGG CTCTTCAGTT CCTCAAGACC AGCAGTCAAC CAACATATTT 
CTTTCCCAGA GTCCCATGAA TAATCTTCAG ACTAACACAG TAGCCCAAGA AGCATTTTTT 
GCAGCACCGA ACTCAATTTC TCCACTTCAG TCAACATCAA ACAGTGAACA ACAAGCTGCT 
TTCCAACAGC AAGCTCCAAT ATCACACATC CAGACTCCTA TGCTTTCCCA AGAACAGGCA 
CAACCCCCGC AGCAGGGTTT ATTTCAGCCT CAGGTGGCCC TGGGCTCCCT TCCACCTAAT 
CCAATGCCTC AAAGCCAACA AGGAACCATG TTCCAGTCAC AGCACTCAAT AGTTGCCATG 
CAGAGTAACT CTCCATCCCA GGAACAGCAG CAGCAGCAGC AACAGCAGCA GCAACAGCAG 
CAGCAACAAC AACAGAGCAT TTTATTCAGT AATCAGAATA CCATGGCTAC AATGGCGTCT 
CCAAAGCAAC CACCACCAAA CATGATATTC AACCCAAATC AAAATCCAAT GGCTAATCAG 
GAGCAACAGA ACCAGTCAAT TTTTCACCAA CAAAGTAACA TGGCCCCAAT GAATCAAGAG 
CAACAGCCCA TGCAATTTCA GAGTCAGTCC ACAGTTTCCT CACTTCAGAA CCCAGGTCCT 
ACCCAGTCGG AATCATCACA GACCCCCTTG TTCCATAGCT CTCCTCAGAT TCAGTTGGTA 
CAAGGGTCAC CTAGTTCTCA AGAGCAGCAA GTAACTCTCT TCTTATCTCC AGCATCCATG 
TCTGCCTTGC AGACCAGTAT AAATCAACAA GATATGCAAC AGTCTCCTCT TTATTCCCCT 
CAGAACAACA TGCCTGGAAT TCAAGGAGCC ACATCTTCGC CTCAACCACA GGCTACTTTA 
TTTCACAACA CAGCAGGAGG CACAATGAAC CAACTGCAGA ATTCTCCTGG CTCATCTCAG 
CAGACATCAG GAATGTTCTT ATTTGGCATT CAAAATAACT GTAGTCAGCT TTTAACCTCT 
GGACCAGCTA CATTGCCTGA TCAGTTGATG GCCATAAGTC AGCCAGGCCA ACCACAAAAC 
GAGGGCCAGC CACCTGTGAC AACACTTCTT TCTCAGCAAA TGCCAGAGAA TTCTCCACTG 
GCATCCTCTA TAAACACCAA CCAGAACATC GAAAAGATTG ATTTGCTTGT TTCATTGCAA 
AACCAAGGGA ACAACTTGAC TGGCTCCTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_011522817.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521119.1 Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu64333
Clone ID Related Accession (Same CDS sequence)	XM_011522817.3 , XM_011522817.2
Accession Version	XM_011522817.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTCTACTCGC GAGAATCTGT CTATGATCTT CTCCCAAAGG AGTTACAGTT ACCTCCATCT 
AGAGAAACAT CTGTAGCATC AATGAGTCAG ACAAGCGGTG GTGAGGCAGG CTCGCCTCCT 
CCAGCTGTTG TTGCTGCTGA TGCTTCTTCA GCTCCCTCCT CTTCCTCCAT GGGCGGTGCT 
TGCAGCTCCT TTACCACCTC TTCCAGCCCT ACCATTTATT CTACCTCAGT CACCGACAGC 
AAGGCTATGC AAGTGGAGAG CTGCTCCTCA GCCGTGGGGG TAAGTAACAG AGGGGTAAGT 
GAAAAGCAGT TAACCAGTAA CACAGTTCAG CAGCATCCAT CAACACCGAA GAGGCACACA 
GTCTTGTACA TCTCACCACC ACCTGAGGAC TTGCTGGATA ACAGTCGGAT GTCCTGCCAG 
GATGAGGGGT GTGGATTGGA ATCTGAGCAG AGCTGCAGTA TGTGGATGGA GGATTCCCCC 
TCCAACTTCA GTAACATGAG CACCAGTTCC TACAATGATA ACACTGAGGT ACCTCGTAAA 
TCACGAAAAC GAAATCCAAA GCAGAGGCCG GGGGTCAAAC GACGAGATTG TGAAGAATCT 
AATATGGATA TATTTGATGC CGACAGTGCC AAAGCACCTC ACTATGTGCT TTCTCAGCTT 
ACCACGGACA ACAAAGGCAA CTCAAAAGCG GGAAATGGAA CATTGGAAAA CCAAAAAGGA 
ACTGGAGTAA AGAAGAGCCC TATGTTGTGT GGACAATATC CTGTTAAAAG TGAGGGAAAG 
GAGCTGAAGA TAGTTGTACA ACCTGAGACA CAGCACCGAG CTCGGTACCT GACTGAGGGC 
AGCCGTGGCT CAGTGAAAGA TAGAACACAG CAAGGCTTTC CTACAGTAAA GCTGGAAGGC 
CATAATGAAC CTGTAGTGTT GCAAGTGTTT GTGGGCAACG ACTCTGGACG AGTGAAACCA 
CATGGATTTT ATCAGGCCTG CAGAGTAACT GGACGAAATA CAACTCCTTG CAAAGAAGTG 
GACATTGAAG GCACTACTGT TATAGAAGTC GGCCTTGATC CTAGCAACAA CATGACACTG 
GCGGTGGACT GCGTAGGGAT ATTGAAATTG AGGAATGCTG ATGTCGAAGC CAGAATAGGA 
ATTGCTGGTT CCAAGAAGAA AAGCACTCGT GCCAGATTGG TTTTTCGAGT TAATATCATG 
AGGAAAGATG GCTCCACTTT GACACTGCAA ACACCCTCTT CTCCAATTTT GTGTACTCAG 
CCAGCAGGAG TGCCAGAAAT CTTAAAGAAA AGCTTGCATA GCTGTTCAGT GAAAGGAGAA 
GAAGAAGTGT TTTTAATCGG CAAGAACTTT CTGAAAGGAA CTAAAGTTAT TTTCCAAGAA 
AATGTTTCTG ATGAAAACTC TTGGAAGTCA GAAGCTGAAA TTGATATGGA ACTATTTCAT 
CAGAATCATC TTATTGTGAA GGTTCCTCCC TATCATGACC AACATATAAC TTTGCCTGTG 
TCAGTGGGAA TATATGTAGT GACAAATGCT GGAAGATCTC ATGATGTTCA ACCATTCACT 
TACACTCCAG ACCCAGCAGC TGGTGCTTTG AATGTAAATG TGAAGAAGGA AATATCTAGT 
CCAGCAAGAC CTTGCTCTTT TGAAGAGGCC ATGAAAGCAA TGAAAACTAC TGGATGTAAT 
TTAGATAAGG TAAATATTAT CCCTAATGCC CTGATGACTC CACTCATACC AAGCAGTATG 
ATTAAGAGTG AAGATGTTAC TCCAATGGAA GTAACAGCAG AAAAAAGATC TTCCACTATT 
TTTAAGACTA CAAAGTCTGT TGGATCAACT CAGCAAACAT TAGAAAACAT CTCAAACATA 
GCAGGAAATG GCTCTTTTTC ATCACCATCA TCTTCCCACC TACCTTCTGA AAATGAAAAA 
CAGCAGCAGA TTCAGCCCAA GGCATACAAC CCAGAGACCC TGACAACTAT TCAAACCCAG 
GACATCTCAC AGCCTGGTAC TTTTCCAGCA GTTTCTGCTT CTAGTCAGCT GCCCAACAGC 
GATGCACTAT TGCAGCAGGC TACACAGTTT CAGACAAGAG AAACTCAGTC TAGAGAGATA 
TTACAGTCAG ATGGTACAGT GGTTAATTTG TCACAACTGA CTGAGGCATC ACAACAACAG 
CAGCAGTCAC CACTACAAGA ACAAGCACAG ACTTTACAGC AGCAGATTTC ATCAAATATT 
TTTCCATCAC CAAATAGTGT GAGTCAGCTT CAGAATACTA TTCAGCAGCT GCAAGCAGGG 
AGTTTCACAG GCAGTACTGC TAGTGGCAGC AGTGGAAGTG TTGACTTGGT CCAACAAGTT 
TTAGAGGCAC AGCAGCAGTT ATCTTCAGTT TTATTTTCTG CTCCAGATGG TAATGAGAAT 
GTTCAAGAGC AGCTTAGTGC AGATATTTTT CAACAAGTCA GTCAAATTCA GAGTGGTGTA 
AGCCCTGGAA TGTTTTCCTC AACAGAGCCA ACAGTCCATA CCAGACCAGA TAATTTATTA 
CCTGGAAGAG CTGAAAGTGT TCATCCACAG TCTGAAAACA CGTTATCTAA TCAACAGCAG 
CAGCAGCAGC AGCAACAGCA AGTGATGGAA TCTTCAGCCG CAATGGTGAT GGAGATGCAA 
CAGAGTATCT GCCAGGCAGC TGCCCAGATT CAGTCAGAGT TATTCCCTTC AACTGCTTCA 
GCAAATGGAA ACCTTCAGCA ATCGCCAGTT TACCAGCAGA CTTCTCACAT GATGAGTGCA 
TTGTCTACCA ATGAGGATAT GCAAATGCAG TGTGAATTGT TTTCTTCTCC TCCTGCAGTT 
TCTGGAAATG AAACTTCTAC AACTACCACA CAGCAGGTTG CAACCCCTGG CACTACCATG 
TTTCAGACAT CAAGTTCAGG AGATGGAGAA GAAACTGGAA CACAAGCAAA ACAGATTCAG 
AACAGTGTCT TTCAGACCAT GGTCCAAATG CAACATAGTG GGGACAATCA ACCTCAAGTT 
AACCTTTTTT CATCCACAAA AAGTATGATG AGTGTTCAGA ATAGTGGTAC CCAACAACAA 
GGTAATGGTT TATTCCAGCA AGGGAATGAG ATGATGTCAC TTCAATCTGG AAATTTTTTG 
CAGCAGTCTT CTCATTCACA GGCCCAACTT TTTCATCCTC AAAATCCTAT TGCCGATGCT 
CAGAACCTTT CCCAGGAAAC TCAAGGTTCT CTCTTTCATA GTCCAAATCC TATTGTCCAC 
AGTCAGACTT CTACAACCTC CTCTGAACAA ATGCAGCCTC CAATGTTTCA CTCTCAAAGT 
ACCATTGCTG TGTTACAGGG CTCTTCAGTT CCTCAAGACC AGCAGTCAAC CAACATATTT 
CTTTCCCAGA GTCCCATGAA TAATCTTCAG ACTAACACAG TAGCCCAAGA AGCATTTTTT 
GCAGCACCGA ACTCAATTTC TCCACTTCAG TCAACATCAA ACAGTGAACA ACAAGCTGCT 
TTCCAACAGC AAGCTCCAAT ATCACACATC CAGACTCCTA TGCTTTCCCA AGAACAGGCA 
CAACCCCCGC AGCAGGGTTT ATTTCAGCCT CAGGTGGCCC TGGGCTCCCT TCCACCTAAT 
CCAATGCCTC AAAGCCAACA AGGAACCATG TTCCAGTCAC AGCACTCAAT AGTTGCCATG 
CAGAGTAACT CTCCATCCCA GGAACAGCAG CAGCAGCAGC AACAGCAGCA GCAACAGCAG 
CAGCAACAAC AACAGAGCAT TTTATTCAGT AATCAGAATA CCATGGCTAC AATGGCGTCT 
CCAAAGCAAC CACCACCAAA CATGATATTC AACCCAAATC AAAATCCAAT GGCTAATCAG 
GAGCAACAGA ACCAGTCAAT TTTTCACCAA CAAAGTAACA TGGCCCCAAT GAATCAAGAG 
CAACAGCCCA TGCAATTTCA GAGTCAGTCC ACAGTTTCCT CACTTCAGAA CCCAGGTCCT 
ACCCAGTCGG AATCATCACA GACCCCCTTG TTCCATAGCT CTCCTCAGAT TCAGTTGGTA 
CAAGGGTCAC CTAGTTCTCA AGAGCAGCAA GTAACTCTCT TCTTATCTCC AGCATCCATG 
TCTGCCTTGC AGACCAGTAT AAATCAACAA GATATGCAAC AGTCTCCTCT TTATTCCCCT 
CAGAACAACA TGCCTGGAAT TCAAGGAGCC ACATCTTCGC CTCAACCACA GGCTACTTTA 
TTTCACAACA CAGCAGGAGG CACAATGAAC CAACTGCAGA ATTCTCCTGG CTCATCTCAG 
CAGACATCAG GAATGTTCTT ATTTGGCATT CAAAATAACT GTAGTCAGCT TTTAACCTCT 
GGACCAGCTA CATTGCCTGA TCAGTTGATG GCCATAAGTC AGCCAGGCCA ACCACAAAAC 
GAGGGCCAGC CACCTGTGAC AACACTTCTT TCTCAGCAAA TGCCAGAGAA TTCTCCACTG 
GCATCCTCTA TAAACACCAA CCAGAACATC GAAAAGATTG ATTTGCTTGT TTCATTGCAA 
AACCAAGGGA ACAACTTGAC TGGCTCCTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521119.1
CDS	337..4929
Translation	MPSDFISLLSADLDLESPKSLYSRESVYDLLPKELQLPPSRETSVASMSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_011522817.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522817.2

ORF Insert Sequence:

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTCTACTCGC GAGAATCTGT CTATGATCTT CTCCCAAAGG AGTTACAGTT ACCTCCATCT 
AGAGAAACAT CTGTAGCATC AATGAGTCAG ACAAGCGGTG GTGAGGCAGG CTCGCCTCCT 
CCAGCTGTTG TTGCTGCTGA TGCTTCTTCA GCTCCCTCCT CTTCCTCCAT GGGCGGTGCT 
TGCAGCTCCT TTACCACCTC TTCCAGCCCT ACCATTTATT CTACCTCAGT CACCGACAGC 
AAGGCTATGC AAGTGGAGAG CTGCTCCTCA GCCGTGGGGG TAAGTAACAG AGGGGTAAGT 
GAAAAGCAGT TAACCAGTAA CACAGTTCAG CAGCATCCAT CAACACCGAA GAGGCACACA 
GTCTTGTACA TCTCACCACC ACCTGAGGAC TTGCTGGATA ACAGTCGGAT GTCCTGCCAG 
GATGAGGGGT GTGGATTGGA ATCTGAGCAG AGCTGCAGTA TGTGGATGGA GGATTCCCCC 
TCCAACTTCA GTAACATGAG CACCAGTTCC TACAATGATA ACACTGAGGT ACCTCGTAAA 
TCACGAAAAC GAAATCCAAA GCAGAGGCCG GGGGTCAAAC GACGAGATTG TGAAGAATCT 
AATATGGATA TATTTGATGC CGACAGTGCC AAAGCACCTC ACTATGTGCT TTCTCAGCTT 
ACCACGGACA ACAAAGGCAA CTCAAAAGCG GGAAATGGAA CATTGGAAAA CCAAAAAGGA 
ACTGGAGTAA AGAAGAGCCC TATGTTGTGT GGACAATATC CTGTTAAAAG TGAGGGAAAG 
GAGCTGAAGA TAGTTGTACA ACCTGAGACA CAGCACCGAG CTCGGTACCT GACTGAGGGC 
AGCCGTGGCT CAGTGAAAGA TAGAACACAG CAAGGCTTTC CTACAGTAAA GCTGGAAGGC 
CATAATGAAC CTGTAGTGTT GCAAGTGTTT GTGGGCAACG ACTCTGGACG AGTGAAACCA 
CATGGATTTT ATCAGGCCTG CAGAGTAACT GGACGAAATA CAACTCCTTG CAAAGAAGTG 
GACATTGAAG GCACTACTGT TATAGAAGTC GGCCTTGATC CTAGCAACAA CATGACACTG 
GCGGTGGACT GCGTAGGGAT ATTGAAATTG AGGAATGCTG ATGTCGAAGC CAGAATAGGA 
ATTGCTGGTT CCAAGAAGAA AAGCACTCGT GCCAGATTGG TTTTTCGAGT TAATATCATG 
AGGAAAGATG GCTCCACTTT GACACTGCAA ACACCCTCTT CTCCAATTTT GTGTACTCAG 
CCAGCAGGAG TGCCAGAAAT CTTAAAGAAA AGCTTGCATA GCTGTTCAGT GAAAGGAGAA 
GAAGAAGTGT TTTTAATCGG CAAGAACTTT CTGAAAGGAA CTAAAGTTAT TTTCCAAGAA 
AATGTTTCTG ATGAAAACTC TTGGAAGTCA GAAGCTGAAA TTGATATGGA ACTATTTCAT 
CAGAATCATC TTATTGTGAA GGTTCCTCCC TATCATGACC AACATATAAC TTTGCCTGTG 
TCAGTGGGAA TATATGTAGT GACAAATGCT GGAAGATCTC ATGATGTTCA ACCATTCACT 
TACACTCCAG ACCCAGCAGC TGGTGCTTTG AATGTAAATG TGAAGAAGGA AATATCTAGT 
CCAGCAAGAC CTTGCTCTTT TGAAGAGGCC ATGAAAGCAA TGAAAACTAC TGGATGTAAT 
TTAGATAAGG TAAATATTAT CCCTAATGCC CTGATGACTC CACTCATACC AAGCAGTATG 
ATTAAGAGTG AAGATGTTAC TCCAATGGAA GTAACAGCAG AAAAAAGATC TTCCACTATT 
TTTAAGACTA CAAAGTCTGT TGGATCAACT CAGCAAACAT TAGAAAACAT CTCAAACATA 
GCAGGAAATG GCTCTTTTTC ATCACCATCA TCTTCCCACC TACCTTCTGA AAATGAAAAA 
CAGCAGCAGA TTCAGCCCAA GGCATACAAC CCAGAGACCC TGACAACTAT TCAAACCCAG 
GACATCTCAC AGCCTGGTAC TTTTCCAGCA GTTTCTGCTT CTAGTCAGCT GCCCAACAGC 
GATGCACTAT TGCAGCAGGC TACACAGTTT CAGACAAGAG AAACTCAGTC TAGAGAGATA 
TTACAGTCAG ATGGTACAGT GGTTAATTTG TCACAACTGA CTGAGGCATC ACAACAACAG 
CAGCAGTCAC CACTACAAGA ACAAGCACAG ACTTTACAGC AGCAGATTTC ATCAAATATT 
TTTCCATCAC CAAATAGTGT GAGTCAGCTT CAGAATACTA TTCAGCAGCT GCAAGCAGGG 
AGTTTCACAG GCAGTACTGC TAGTGGCAGC AGTGGAAGTG TTGACTTGGT CCAACAAGTT 
TTAGAGGCAC AGCAGCAGTT ATCTTCAGTT TTATTTTCTG CTCCAGATGG TAATGAGAAT 
GTTCAAGAGC AGCTTAGTGC AGATATTTTT CAACAAGTCA GTCAAATTCA GAGTGGTGTA 
AGCCCTGGAA TGTTTTCCTC AACAGAGCCA ACAGTCCATA CCAGACCAGA TAATTTATTA 
CCTGGAAGAG CTGAAAGTGT TCATCCACAG TCTGAAAACA CGTTATCTAA TCAACAGCAG 
CAGCAGCAGC AGCAACAGCA AGTGATGGAA TCTTCAGCCG CAATGGTGAT GGAGATGCAA 
CAGAGTATCT GCCAGGCAGC TGCCCAGATT CAGTCAGAGT TATTCCCTTC AACTGCTTCA 
GCAAATGGAA ACCTTCAGCA ATCGCCAGTT TACCAGCAGA CTTCTCACAT GATGAGTGCA 
TTGTCTACCA ATGAGGATAT GCAAATGCAG TGTGAATTGT TTTCTTCTCC TCCTGCAGTT 
TCTGGAAATG AAACTTCTAC AACTACCACA CAGCAGGTTG CAACCCCTGG CACTACCATG 
TTTCAGACAT CAAGTTCAGG AGATGGAGAA GAAACTGGAA CACAAGCAAA ACAGATTCAG 
AACAGTGTCT TTCAGACCAT GGTCCAAATG CAACATAGTG GGGACAATCA ACCTCAAGTT 
AACCTTTTTT CATCCACAAA AAGTATGATG AGTGTTCAGA ATAGTGGTAC CCAACAACAA 
GGTAATGGTT TATTCCAGCA AGGGAATGAG ATGATGTCAC TTCAATCTGG AAATTTTTTG 
CAGCAGTCTT CTCATTCACA GGCCCAACTT TTTCATCCTC AAAATCCTAT TGCCGATGCT 
CAGAACCTTT CCCAGGAAAC TCAAGGTTCT CTCTTTCATA GTCCAAATCC TATTGTCCAC 
AGTCAGACTT CTACAACCTC CTCTGAACAA ATGCAGCCTC CAATGTTTCA CTCTCAAAGT 
ACCATTGCTG TGTTACAGGG CTCTTCAGTT CCTCAAGACC AGCAGTCAAC CAACATATTT 
CTTTCCCAGA GTCCCATGAA TAATCTTCAG ACTAACACAG TAGCCCAAGA AGCATTTTTT 
GCAGCACCGA ACTCAATTTC TCCACTTCAG TCAACATCAA ACAGTGAACA ACAAGCTGCT 
TTCCAACAGC AAGCTCCAAT ATCACACATC CAGACTCCTA TGCTTTCCCA AGAACAGGCA 
CAACCCCCGC AGCAGGGTTT ATTTCAGCCT CAGGTGGCCC TGGGCTCCCT TCCACCTAAT 
CCAATGCCTC AAAGCCAACA AGGAACCATG TTCCAGTCAC AGCACTCAAT AGTTGCCATG 
CAGAGTAACT CTCCATCCCA GGAACAGCAG CAGCAGCAGC AACAGCAGCA GCAACAGCAG 
CAGCAACAAC AACAGAGCAT TTTATTCAGT AATCAGAATA CCATGGCTAC AATGGCGTCT 
CCAAAGCAAC CACCACCAAA CATGATATTC AACCCAAATC AAAATCCAAT GGCTAATCAG 
GAGCAACAGA ACCAGTCAAT TTTTCACCAA CAAAGTAACA TGGCCCCAAT GAATCAAGAG 
CAACAGCCCA TGCAATTTCA GAGTCAGTCC ACAGTTTCCT CACTTCAGAA CCCAGGTCCT 
ACCCAGTCGG AATCATCACA GACCCCCTTG TTCCATAGCT CTCCTCAGAT TCAGTTGGTA 
CAAGGGTCAC CTAGTTCTCA AGAGCAGCAA GTAACTCTCT TCTTATCTCC AGCATCCATG 
TCTGCCTTGC AGACCAGTAT AAATCAACAA GATATGCAAC AGTCTCCTCT TTATTCCCCT 
CAGAACAACA TGCCTGGAAT TCAAGGAGCC ACATCTTCGC CTCAACCACA GGCTACTTTA 
TTTCACAACA CAGCAGGAGG CACAATGAAC CAACTGCAGA ATTCTCCTGG CTCATCTCAG 
CAGACATCAG GAATGTTCTT ATTTGGCATT CAAAATAACT GTAGTCAGCT TTTAACCTCT 
GGACCAGCTA CATTGCCTGA TCAGTTGATG GCCATAAGTC AGCCAGGCCA ACCACAAAAC 
GAGGGCCAGC CACCTGTGAC AACACTTCTT TCTCAGCAAA TGCCAGAGAA TTCTCCACTG 
GCATCCTCTA TAAACACCAA CCAGAACATC GAAAAGATTG ATTTGCTTGT TTCATTGCAA 
AACCAAGGGA ACAACTTGAC TGGCTCCTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_011522819.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521121.1 Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X6, mRNA.

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CloneID	OHu64334
Clone ID Related Accession (Same CDS sequence)	XM_011522819.2 , XM_017022872.1 , XM_011522819.3 , XM_011522820.2 , XM_011522822.3 , XM_011522822.2 , XM_017022872.2 , XM_011522820.3
Accession Version	XM_011522819.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522819.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT CTAGTCCAGC AAGACCTTGC 
TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT GTAATTTAGA TAAGGTAAAT 
ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA GTATGATTAA GAGTGAAGAT 
GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA CTATTTTTAA GACTACAAAG 
TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA ACATAGCAGG AAATGGCTCT 
TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG AAAAACAGCA GCAGATTCAG 
CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA CCCAGGACAT CTCACAGCCT 
GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA ACAGCGATGC ACTATTGCAG 
CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG AGATATTACA GTCAGATGGT 
ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC AACAGCAGCA GTCACCACTA 
CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA ATATTTTTCC ATCACCAAAT 
AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG CAGGGAGTTT CACAGGCAGT 
ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC AAGTTTTAGA GGCACAGCAG 
CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG AGAATGTTCA AGAGCAGCTT 
AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG GTGTAAGCCC TGGAATGTTT 
TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT TATTACCTGG AAGAGCTGAA 
AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC AGCAGCAGCA GCAGCAGCAA 
CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA TGCAACAGAG TATCTGCCAG 
GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG CTTCAGCAAA TGGAAACCTT 
CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA GTGCATTGTC TACCAATGAG 
GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG CAGTTTCTGG AAATGAAACT 
TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA CCATGTTTCA GACATCAAGT 
TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA TTCAGAACAG TGTCTTTCAG 
ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC AAGTTAACCT TTTTTCATCC 
ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC AACAAGGTAA TGGTTTATTC 
CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT TTTTGCAGCA GTCTTCTCAT 
TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG ATGCTCAGAA CCTTTCCCAG 
GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG TCCACAGTCA GACTTCTACA 
ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC AAAGTACCAT TGCTGTGTTA 
CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA TATTTCTTTC CCAGAGTCCC 
ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT TTTTTGCAGC ACCGAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG CTGCTTTCCA ACAGCAAGCT 
CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC AGGCACAACC CCCGCAGCAG 
GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC CTAATCCAAT GCCTCAAAGC 
CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG CCATGCAGAG TAACTCTCCA 
TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA ACAACAACAG 
AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG CGTCTCCAAA GCAACCACCA 
CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA ATCAGGAGCA ACAGAACCAG 
TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC AAGAGCAACA GCCCATGCAA 
TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG GTCCTACCCA GTCGGAATCA 
TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT TGGTACAAGG GTCACCTAGT 
TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT CCATGTCTGC CTTGCAGACC 
AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT CCCCTCAGAA CAACATGCCT 
GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA CTTTATTTCA CAACACAGCA 
GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT CTCAGCAGAC ATCAGGAATG 
TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CCTCTGGACC AGCTACATTG 
CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC AAAACGAGGG CCAGCCACCT 
GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CACTGGCATC CTCTATAAAC 
ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC 
TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521121.1
CDS	726..4703
Translation	MLLQLPPLPPWAVLAAPLPPLPALPFILPQSPTARLCKWRAAPQPWGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_011522819.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522819.2

ORF Insert Sequence:

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT CTAGTCCAGC AAGACCTTGC 
TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT GTAATTTAGA TAAGGTAAAT 
ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA GTATGATTAA GAGTGAAGAT 
GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA CTATTTTTAA GACTACAAAG 
TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA ACATAGCAGG AAATGGCTCT 
TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG AAAAACAGCA GCAGATTCAG 
CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA CCCAGGACAT CTCACAGCCT 
GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA ACAGCGATGC ACTATTGCAG 
CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG AGATATTACA GTCAGATGGT 
ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC AACAGCAGCA GTCACCACTA 
CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA ATATTTTTCC ATCACCAAAT 
AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG CAGGGAGTTT CACAGGCAGT 
ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC AAGTTTTAGA GGCACAGCAG 
CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG AGAATGTTCA AGAGCAGCTT 
AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG GTGTAAGCCC TGGAATGTTT 
TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT TATTACCTGG AAGAGCTGAA 
AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC AGCAGCAGCA GCAGCAGCAA 
CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA TGCAACAGAG TATCTGCCAG 
GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG CTTCAGCAAA TGGAAACCTT 
CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA GTGCATTGTC TACCAATGAG 
GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG CAGTTTCTGG AAATGAAACT 
TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA CCATGTTTCA GACATCAAGT 
TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA TTCAGAACAG TGTCTTTCAG 
ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC AAGTTAACCT TTTTTCATCC 
ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC AACAAGGTAA TGGTTTATTC 
CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT TTTTGCAGCA GTCTTCTCAT 
TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG ATGCTCAGAA CCTTTCCCAG 
GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG TCCACAGTCA GACTTCTACA 
ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC AAAGTACCAT TGCTGTGTTA 
CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA TATTTCTTTC CCAGAGTCCC 
ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT TTTTTGCAGC ACCGAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG CTGCTTTCCA ACAGCAAGCT 
CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC AGGCACAACC CCCGCAGCAG 
GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC CTAATCCAAT GCCTCAAAGC 
CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG CCATGCAGAG TAACTCTCCA 
TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA ACAACAACAG 
AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG CGTCTCCAAA GCAACCACCA 
CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA ATCAGGAGCA ACAGAACCAG 
TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC AAGAGCAACA GCCCATGCAA 
TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG GTCCTACCCA GTCGGAATCA 
TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT TGGTACAAGG GTCACCTAGT 
TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT CCATGTCTGC CTTGCAGACC 
AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT CCCCTCAGAA CAACATGCCT 
GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA CTTTATTTCA CAACACAGCA 
GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT CTCAGCAGAC ATCAGGAATG 
TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CCTCTGGACC AGCTACATTG 
CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC AAAACGAGGG CCAGCCACCT 
GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CACTGGCATC CTCTATAAAC 
ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC 
TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_017022872.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878361.1 Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu64334
Clone ID Related Accession (Same CDS sequence)	XM_011522819.2 , XM_017022872.1 , XM_011522819.3 , XM_011522820.2 , XM_011522822.3 , XM_011522822.2 , XM_017022872.2 , XM_011522820.3
Accession Version	XM_017022872.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT CTAGTCCAGC AAGACCTTGC 
TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT GTAATTTAGA TAAGGTAAAT 
ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA GTATGATTAA GAGTGAAGAT 
GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA CTATTTTTAA GACTACAAAG 
TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA ACATAGCAGG AAATGGCTCT 
TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG AAAAACAGCA GCAGATTCAG 
CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA CCCAGGACAT CTCACAGCCT 
GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA ACAGCGATGC ACTATTGCAG 
CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG AGATATTACA GTCAGATGGT 
ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC AACAGCAGCA GTCACCACTA 
CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA ATATTTTTCC ATCACCAAAT 
AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG CAGGGAGTTT CACAGGCAGT 
ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC AAGTTTTAGA GGCACAGCAG 
CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG AGAATGTTCA AGAGCAGCTT 
AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG GTGTAAGCCC TGGAATGTTT 
TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT TATTACCTGG AAGAGCTGAA 
AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC AGCAGCAGCA GCAGCAGCAA 
CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA TGCAACAGAG TATCTGCCAG 
GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG CTTCAGCAAA TGGAAACCTT 
CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA GTGCATTGTC TACCAATGAG 
GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG CAGTTTCTGG AAATGAAACT 
TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA CCATGTTTCA GACATCAAGT 
TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA TTCAGAACAG TGTCTTTCAG 
ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC AAGTTAACCT TTTTTCATCC 
ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC AACAAGGTAA TGGTTTATTC 
CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT TTTTGCAGCA GTCTTCTCAT 
TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG ATGCTCAGAA CCTTTCCCAG 
GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG TCCACAGTCA GACTTCTACA 
ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC AAAGTACCAT TGCTGTGTTA 
CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA TATTTCTTTC CCAGAGTCCC 
ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT TTTTTGCAGC ACCGAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG CTGCTTTCCA ACAGCAAGCT 
CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC AGGCACAACC CCCGCAGCAG 
GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC CTAATCCAAT GCCTCAAAGC 
CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG CCATGCAGAG TAACTCTCCA 
TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA ACAACAACAG 
AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG CGTCTCCAAA GCAACCACCA 
CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA ATCAGGAGCA ACAGAACCAG 
TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC AAGAGCAACA GCCCATGCAA 
TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG GTCCTACCCA GTCGGAATCA 
TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT TGGTACAAGG GTCACCTAGT 
TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT CCATGTCTGC CTTGCAGACC 
AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT CCCCTCAGAA CAACATGCCT 
GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA CTTTATTTCA CAACACAGCA 
GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT CTCAGCAGAC ATCAGGAATG 
TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CCTCTGGACC AGCTACATTG 
CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC AAAACGAGGG CCAGCCACCT 
GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CACTGGCATC CTCTATAAAC 
ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC 
TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878361.1
CDS	885..4862
Translation	MLLQLPPLPPWAVLAAPLPPLPALPFILPQSPTARLCKWRAAPQPWGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_017022872.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022872.1

ORF Insert Sequence:

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT CTAGTCCAGC AAGACCTTGC 
TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT GTAATTTAGA TAAGGTAAAT 
ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA GTATGATTAA GAGTGAAGAT 
GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA CTATTTTTAA GACTACAAAG 
TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA ACATAGCAGG AAATGGCTCT 
TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG AAAAACAGCA GCAGATTCAG 
CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA CCCAGGACAT CTCACAGCCT 
GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA ACAGCGATGC ACTATTGCAG 
CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG AGATATTACA GTCAGATGGT 
ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC AACAGCAGCA GTCACCACTA 
CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA ATATTTTTCC ATCACCAAAT 
AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG CAGGGAGTTT CACAGGCAGT 
ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC AAGTTTTAGA GGCACAGCAG 
CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG AGAATGTTCA AGAGCAGCTT 
AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG GTGTAAGCCC TGGAATGTTT 
TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT TATTACCTGG AAGAGCTGAA 
AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC AGCAGCAGCA GCAGCAGCAA 
CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA TGCAACAGAG TATCTGCCAG 
GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG CTTCAGCAAA TGGAAACCTT 
CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA GTGCATTGTC TACCAATGAG 
GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG CAGTTTCTGG AAATGAAACT 
TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA CCATGTTTCA GACATCAAGT 
TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA TTCAGAACAG TGTCTTTCAG 
ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC AAGTTAACCT TTTTTCATCC 
ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC AACAAGGTAA TGGTTTATTC 
CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT TTTTGCAGCA GTCTTCTCAT 
TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG ATGCTCAGAA CCTTTCCCAG 
GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG TCCACAGTCA GACTTCTACA 
ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC AAAGTACCAT TGCTGTGTTA 
CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA TATTTCTTTC CCAGAGTCCC 
ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT TTTTTGCAGC ACCGAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG CTGCTTTCCA ACAGCAAGCT 
CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC AGGCACAACC CCCGCAGCAG 
GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC CTAATCCAAT GCCTCAAAGC 
CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG CCATGCAGAG TAACTCTCCA 
TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA ACAACAACAG 
AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG CGTCTCCAAA GCAACCACCA 
CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA ATCAGGAGCA ACAGAACCAG 
TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC AAGAGCAACA GCCCATGCAA 
TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG GTCCTACCCA GTCGGAATCA 
TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT TGGTACAAGG GTCACCTAGT 
TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT CCATGTCTGC CTTGCAGACC 
AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT CCCCTCAGAA CAACATGCCT 
GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA CTTTATTTCA CAACACAGCA 
GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT CTCAGCAGAC ATCAGGAATG 
TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CCTCTGGACC AGCTACATTG 
CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC AAAACGAGGG CCAGCCACCT 
GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CACTGGCATC CTCTATAAAC 
ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC 
TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_011522819.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521121.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu64334
Clone ID Related Accession (Same CDS sequence)	XM_011522819.2 , XM_017022872.1 , XM_011522819.3 , XM_011522820.2 , XM_011522822.3 , XM_011522822.2 , XM_017022872.2 , XM_011522820.3
Accession Version	XM_011522819.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011522819.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT CTAGTCCAGC AAGACCTTGC 
TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT GTAATTTAGA TAAGGTAAAT 
ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA GTATGATTAA GAGTGAAGAT 
GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA CTATTTTTAA GACTACAAAG 
TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA ACATAGCAGG AAATGGCTCT 
TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG AAAAACAGCA GCAGATTCAG 
CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA CCCAGGACAT CTCACAGCCT 
GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA ACAGCGATGC ACTATTGCAG 
CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG AGATATTACA GTCAGATGGT 
ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC AACAGCAGCA GTCACCACTA 
CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA ATATTTTTCC ATCACCAAAT 
AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG CAGGGAGTTT CACAGGCAGT 
ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC AAGTTTTAGA GGCACAGCAG 
CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG AGAATGTTCA AGAGCAGCTT 
AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG GTGTAAGCCC TGGAATGTTT 
TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT TATTACCTGG AAGAGCTGAA 
AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC AGCAGCAGCA GCAGCAGCAA 
CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA TGCAACAGAG TATCTGCCAG 
GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG CTTCAGCAAA TGGAAACCTT 
CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA GTGCATTGTC TACCAATGAG 
GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG CAGTTTCTGG AAATGAAACT 
TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA CCATGTTTCA GACATCAAGT 
TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA TTCAGAACAG TGTCTTTCAG 
ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC AAGTTAACCT TTTTTCATCC 
ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC AACAAGGTAA TGGTTTATTC 
CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT TTTTGCAGCA GTCTTCTCAT 
TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG ATGCTCAGAA CCTTTCCCAG 
GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG TCCACAGTCA GACTTCTACA 
ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC AAAGTACCAT TGCTGTGTTA 
CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA TATTTCTTTC CCAGAGTCCC 
ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT TTTTTGCAGC ACCGAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG CTGCTTTCCA ACAGCAAGCT 
CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC AGGCACAACC CCCGCAGCAG 
GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC CTAATCCAAT GCCTCAAAGC 
CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG CCATGCAGAG TAACTCTCCA 
TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA ACAACAACAG 
AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG CGTCTCCAAA GCAACCACCA 
CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA ATCAGGAGCA ACAGAACCAG 
TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC AAGAGCAACA GCCCATGCAA 
TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG GTCCTACCCA GTCGGAATCA 
TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT TGGTACAAGG GTCACCTAGT 
TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT CCATGTCTGC CTTGCAGACC 
AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT CCCCTCAGAA CAACATGCCT 
GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA CTTTATTTCA CAACACAGCA 
GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT CTCAGCAGAC ATCAGGAATG 
TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CCTCTGGACC AGCTACATTG 
CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC AAAACGAGGG CCAGCCACCT 
GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CACTGGCATC CTCTATAAAC 
ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC 
TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521121.1
CDS	726..4703
Translation	MLLQLPPLPPWAVLAAPLPPLPALPFILPQSPTARLCKWRAAPQPWGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_011522819.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522819.3

ORF Insert Sequence:

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT CTAGTCCAGC AAGACCTTGC 
TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT GTAATTTAGA TAAGGTAAAT 
ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA GTATGATTAA GAGTGAAGAT 
GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA CTATTTTTAA GACTACAAAG 
TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA ACATAGCAGG AAATGGCTCT 
TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG AAAAACAGCA GCAGATTCAG 
CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA CCCAGGACAT CTCACAGCCT 
GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA ACAGCGATGC ACTATTGCAG 
CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG AGATATTACA GTCAGATGGT 
ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC AACAGCAGCA GTCACCACTA 
CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA ATATTTTTCC ATCACCAAAT 
AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG CAGGGAGTTT CACAGGCAGT 
ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC AAGTTTTAGA GGCACAGCAG 
CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG AGAATGTTCA AGAGCAGCTT 
AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG GTGTAAGCCC TGGAATGTTT 
TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT TATTACCTGG AAGAGCTGAA 
AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC AGCAGCAGCA GCAGCAGCAA 
CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA TGCAACAGAG TATCTGCCAG 
GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG CTTCAGCAAA TGGAAACCTT 
CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA GTGCATTGTC TACCAATGAG 
GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG CAGTTTCTGG AAATGAAACT 
TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA CCATGTTTCA GACATCAAGT 
TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA TTCAGAACAG TGTCTTTCAG 
ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC AAGTTAACCT TTTTTCATCC 
ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC AACAAGGTAA TGGTTTATTC 
CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT TTTTGCAGCA GTCTTCTCAT 
TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG ATGCTCAGAA CCTTTCCCAG 
GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG TCCACAGTCA GACTTCTACA 
ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC AAAGTACCAT TGCTGTGTTA 
CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA TATTTCTTTC CCAGAGTCCC 
ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT TTTTTGCAGC ACCGAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG CTGCTTTCCA ACAGCAAGCT 
CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC AGGCACAACC CCCGCAGCAG 
GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC CTAATCCAAT GCCTCAAAGC 
CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG CCATGCAGAG TAACTCTCCA 
TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA ACAACAACAG 
AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG CGTCTCCAAA GCAACCACCA 
CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA ATCAGGAGCA ACAGAACCAG 
TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC AAGAGCAACA GCCCATGCAA 
TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG GTCCTACCCA GTCGGAATCA 
TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT TGGTACAAGG GTCACCTAGT 
TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT CCATGTCTGC CTTGCAGACC 
AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT CCCCTCAGAA CAACATGCCT 
GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA CTTTATTTCA CAACACAGCA 
GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT CTCAGCAGAC ATCAGGAATG 
TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CCTCTGGACC AGCTACATTG 
CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC AAAACGAGGG CCAGCCACCT 
GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CACTGGCATC CTCTATAAAC 
ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC 
TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_011522820.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521122.1 Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X7, mRNA.

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CloneID	OHu64334
Clone ID Related Accession (Same CDS sequence)	XM_011522819.2 , XM_017022872.1 , XM_011522819.3 , XM_011522820.2 , XM_011522822.3 , XM_011522822.2 , XM_017022872.2 , XM_011522820.3
Accession Version	XM_011522820.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522820.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT CTAGTCCAGC AAGACCTTGC 
TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT GTAATTTAGA TAAGGTAAAT 
ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA GTATGATTAA GAGTGAAGAT 
GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA CTATTTTTAA GACTACAAAG 
TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA ACATAGCAGG AAATGGCTCT 
TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG AAAAACAGCA GCAGATTCAG 
CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA CCCAGGACAT CTCACAGCCT 
GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA ACAGCGATGC ACTATTGCAG 
CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG AGATATTACA GTCAGATGGT 
ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC AACAGCAGCA GTCACCACTA 
CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA ATATTTTTCC ATCACCAAAT 
AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG CAGGGAGTTT CACAGGCAGT 
ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC AAGTTTTAGA GGCACAGCAG 
CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG AGAATGTTCA AGAGCAGCTT 
AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG GTGTAAGCCC TGGAATGTTT 
TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT TATTACCTGG AAGAGCTGAA 
AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC AGCAGCAGCA GCAGCAGCAA 
CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA TGCAACAGAG TATCTGCCAG 
GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG CTTCAGCAAA TGGAAACCTT 
CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA GTGCATTGTC TACCAATGAG 
GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG CAGTTTCTGG AAATGAAACT 
TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA CCATGTTTCA GACATCAAGT 
TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA TTCAGAACAG TGTCTTTCAG 
ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC AAGTTAACCT TTTTTCATCC 
ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC AACAAGGTAA TGGTTTATTC 
CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT TTTTGCAGCA GTCTTCTCAT 
TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG ATGCTCAGAA CCTTTCCCAG 
GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG TCCACAGTCA GACTTCTACA 
ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC AAAGTACCAT TGCTGTGTTA 
CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA TATTTCTTTC CCAGAGTCCC 
ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT TTTTTGCAGC ACCGAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG CTGCTTTCCA ACAGCAAGCT 
CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC AGGCACAACC CCCGCAGCAG 
GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC CTAATCCAAT GCCTCAAAGC 
CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG CCATGCAGAG TAACTCTCCA 
TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA ACAACAACAG 
AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG CGTCTCCAAA GCAACCACCA 
CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA ATCAGGAGCA ACAGAACCAG 
TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC AAGAGCAACA GCCCATGCAA 
TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG GTCCTACCCA GTCGGAATCA 
TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT TGGTACAAGG GTCACCTAGT 
TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT CCATGTCTGC CTTGCAGACC 
AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT CCCCTCAGAA CAACATGCCT 
GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA CTTTATTTCA CAACACAGCA 
GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT CTCAGCAGAC ATCAGGAATG 
TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CCTCTGGACC AGCTACATTG 
CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC AAAACGAGGG CCAGCCACCT 
GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CACTGGCATC CTCTATAAAC 
ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC 
TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521122.1
CDS	156..4133
Translation	MLLQLPPLPPWAVLAAPLPPLPALPFILPQSPTARLCKWRAAPQPWGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_011522820.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522820.2

ORF Insert Sequence:

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT CTAGTCCAGC AAGACCTTGC 
TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT GTAATTTAGA TAAGGTAAAT 
ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA GTATGATTAA GAGTGAAGAT 
GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA CTATTTTTAA GACTACAAAG 
TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA ACATAGCAGG AAATGGCTCT 
TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG AAAAACAGCA GCAGATTCAG 
CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA CCCAGGACAT CTCACAGCCT 
GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA ACAGCGATGC ACTATTGCAG 
CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG AGATATTACA GTCAGATGGT 
ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC AACAGCAGCA GTCACCACTA 
CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA ATATTTTTCC ATCACCAAAT 
AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG CAGGGAGTTT CACAGGCAGT 
ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC AAGTTTTAGA GGCACAGCAG 
CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG AGAATGTTCA AGAGCAGCTT 
AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG GTGTAAGCCC TGGAATGTTT 
TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT TATTACCTGG AAGAGCTGAA 
AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC AGCAGCAGCA GCAGCAGCAA 
CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA TGCAACAGAG TATCTGCCAG 
GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG CTTCAGCAAA TGGAAACCTT 
CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA GTGCATTGTC TACCAATGAG 
GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG CAGTTTCTGG AAATGAAACT 
TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA CCATGTTTCA GACATCAAGT 
TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA TTCAGAACAG TGTCTTTCAG 
ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC AAGTTAACCT TTTTTCATCC 
ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC AACAAGGTAA TGGTTTATTC 
CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT TTTTGCAGCA GTCTTCTCAT 
TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG ATGCTCAGAA CCTTTCCCAG 
GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG TCCACAGTCA GACTTCTACA 
ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC AAAGTACCAT TGCTGTGTTA 
CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA TATTTCTTTC CCAGAGTCCC 
ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT TTTTTGCAGC ACCGAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG CTGCTTTCCA ACAGCAAGCT 
CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC AGGCACAACC CCCGCAGCAG 
GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC CTAATCCAAT GCCTCAAAGC 
CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG CCATGCAGAG TAACTCTCCA 
TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA ACAACAACAG 
AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG CGTCTCCAAA GCAACCACCA 
CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA ATCAGGAGCA ACAGAACCAG 
TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC AAGAGCAACA GCCCATGCAA 
TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG GTCCTACCCA GTCGGAATCA 
TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT TGGTACAAGG GTCACCTAGT 
TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT CCATGTCTGC CTTGCAGACC 
AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT CCCCTCAGAA CAACATGCCT 
GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA CTTTATTTCA CAACACAGCA 
GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT CTCAGCAGAC ATCAGGAATG 
TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CCTCTGGACC AGCTACATTG 
CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC AAAACGAGGG CCAGCCACCT 
GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CACTGGCATC CTCTATAAAC 
ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC 
TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_011522822.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521124.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X9, mRNA.

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CloneID	OHu64334
Clone ID Related Accession (Same CDS sequence)	XM_011522819.2 , XM_017022872.1 , XM_011522819.3 , XM_011522820.2 , XM_011522822.3 , XM_011522822.2 , XM_017022872.2 , XM_011522820.3
Accession Version	XM_011522822.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011522822.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT CTAGTCCAGC AAGACCTTGC 
TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT GTAATTTAGA TAAGGTAAAT 
ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA GTATGATTAA GAGTGAAGAT 
GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA CTATTTTTAA GACTACAAAG 
TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA ACATAGCAGG AAATGGCTCT 
TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG AAAAACAGCA GCAGATTCAG 
CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA CCCAGGACAT CTCACAGCCT 
GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA ACAGCGATGC ACTATTGCAG 
CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG AGATATTACA GTCAGATGGT 
ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC AACAGCAGCA GTCACCACTA 
CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA ATATTTTTCC ATCACCAAAT 
AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG CAGGGAGTTT CACAGGCAGT 
ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC AAGTTTTAGA GGCACAGCAG 
CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG AGAATGTTCA AGAGCAGCTT 
AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG GTGTAAGCCC TGGAATGTTT 
TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT TATTACCTGG AAGAGCTGAA 
AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC AGCAGCAGCA GCAGCAGCAA 
CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA TGCAACAGAG TATCTGCCAG 
GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG CTTCAGCAAA TGGAAACCTT 
CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA GTGCATTGTC TACCAATGAG 
GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG CAGTTTCTGG AAATGAAACT 
TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA CCATGTTTCA GACATCAAGT 
TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA TTCAGAACAG TGTCTTTCAG 
ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC AAGTTAACCT TTTTTCATCC 
ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC AACAAGGTAA TGGTTTATTC 
CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT TTTTGCAGCA GTCTTCTCAT 
TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG ATGCTCAGAA CCTTTCCCAG 
GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG TCCACAGTCA GACTTCTACA 
ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC AAAGTACCAT TGCTGTGTTA 
CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA TATTTCTTTC CCAGAGTCCC 
ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT TTTTTGCAGC ACCGAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG CTGCTTTCCA ACAGCAAGCT 
CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC AGGCACAACC CCCGCAGCAG 
GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC CTAATCCAAT GCCTCAAAGC 
CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG CCATGCAGAG TAACTCTCCA 
TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA ACAACAACAG 
AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG CGTCTCCAAA GCAACCACCA 
CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA ATCAGGAGCA ACAGAACCAG 
TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC AAGAGCAACA GCCCATGCAA 
TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG GTCCTACCCA GTCGGAATCA 
TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT TGGTACAAGG GTCACCTAGT 
TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT CCATGTCTGC CTTGCAGACC 
AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT CCCCTCAGAA CAACATGCCT 
GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA CTTTATTTCA CAACACAGCA 
GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT CTCAGCAGAC ATCAGGAATG 
TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CCTCTGGACC AGCTACATTG 
CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC AAAACGAGGG CCAGCCACCT 
GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CACTGGCATC CTCTATAAAC 
ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC 
TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521124.1
CDS	104..4081
Translation	MLLQLPPLPPWAVLAAPLPPLPALPFILPQSPTARLCKWRAAPQPWGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_011522822.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522822.3

ORF Insert Sequence:

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT CTAGTCCAGC AAGACCTTGC 
TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT GTAATTTAGA TAAGGTAAAT 
ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA GTATGATTAA GAGTGAAGAT 
GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA CTATTTTTAA GACTACAAAG 
TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA ACATAGCAGG AAATGGCTCT 
TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG AAAAACAGCA GCAGATTCAG 
CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA CCCAGGACAT CTCACAGCCT 
GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA ACAGCGATGC ACTATTGCAG 
CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG AGATATTACA GTCAGATGGT 
ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC AACAGCAGCA GTCACCACTA 
CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA ATATTTTTCC ATCACCAAAT 
AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG CAGGGAGTTT CACAGGCAGT 
ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC AAGTTTTAGA GGCACAGCAG 
CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG AGAATGTTCA AGAGCAGCTT 
AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG GTGTAAGCCC TGGAATGTTT 
TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT TATTACCTGG AAGAGCTGAA 
AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC AGCAGCAGCA GCAGCAGCAA 
CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA TGCAACAGAG TATCTGCCAG 
GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG CTTCAGCAAA TGGAAACCTT 
CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA GTGCATTGTC TACCAATGAG 
GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG CAGTTTCTGG AAATGAAACT 
TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA CCATGTTTCA GACATCAAGT 
TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA TTCAGAACAG TGTCTTTCAG 
ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC AAGTTAACCT TTTTTCATCC 
ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC AACAAGGTAA TGGTTTATTC 
CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT TTTTGCAGCA GTCTTCTCAT 
TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG ATGCTCAGAA CCTTTCCCAG 
GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG TCCACAGTCA GACTTCTACA 
ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC AAAGTACCAT TGCTGTGTTA 
CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA TATTTCTTTC CCAGAGTCCC 
ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT TTTTTGCAGC ACCGAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG CTGCTTTCCA ACAGCAAGCT 
CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC AGGCACAACC CCCGCAGCAG 
GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC CTAATCCAAT GCCTCAAAGC 
CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG CCATGCAGAG TAACTCTCCA 
TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA ACAACAACAG 
AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG CGTCTCCAAA GCAACCACCA 
CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA ATCAGGAGCA ACAGAACCAG 
TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC AAGAGCAACA GCCCATGCAA 
TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG GTCCTACCCA GTCGGAATCA 
TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT TGGTACAAGG GTCACCTAGT 
TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT CCATGTCTGC CTTGCAGACC 
AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT CCCCTCAGAA CAACATGCCT 
GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA CTTTATTTCA CAACACAGCA 
GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT CTCAGCAGAC ATCAGGAATG 
TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CCTCTGGACC AGCTACATTG 
CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC AAAACGAGGG CCAGCCACCT 
GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CACTGGCATC CTCTATAAAC 
ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC 
TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_011522822.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521124.1 Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu64334
Clone ID Related Accession (Same CDS sequence)	XM_011522819.2 , XM_017022872.1 , XM_011522819.3 , XM_011522820.2 , XM_011522822.3 , XM_011522822.2 , XM_017022872.2 , XM_011522820.3
Accession Version	XM_011522822.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522822.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT CTAGTCCAGC AAGACCTTGC 
TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT GTAATTTAGA TAAGGTAAAT 
ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA GTATGATTAA GAGTGAAGAT 
GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA CTATTTTTAA GACTACAAAG 
TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA ACATAGCAGG AAATGGCTCT 
TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG AAAAACAGCA GCAGATTCAG 
CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA CCCAGGACAT CTCACAGCCT 
GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA ACAGCGATGC ACTATTGCAG 
CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG AGATATTACA GTCAGATGGT 
ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC AACAGCAGCA GTCACCACTA 
CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA ATATTTTTCC ATCACCAAAT 
AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG CAGGGAGTTT CACAGGCAGT 
ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC AAGTTTTAGA GGCACAGCAG 
CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG AGAATGTTCA AGAGCAGCTT 
AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG GTGTAAGCCC TGGAATGTTT 
TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT TATTACCTGG AAGAGCTGAA 
AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC AGCAGCAGCA GCAGCAGCAA 
CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA TGCAACAGAG TATCTGCCAG 
GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG CTTCAGCAAA TGGAAACCTT 
CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA GTGCATTGTC TACCAATGAG 
GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG CAGTTTCTGG AAATGAAACT 
TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA CCATGTTTCA GACATCAAGT 
TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA TTCAGAACAG TGTCTTTCAG 
ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC AAGTTAACCT TTTTTCATCC 
ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC AACAAGGTAA TGGTTTATTC 
CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT TTTTGCAGCA GTCTTCTCAT 
TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG ATGCTCAGAA CCTTTCCCAG 
GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG TCCACAGTCA GACTTCTACA 
ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC AAAGTACCAT TGCTGTGTTA 
CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA TATTTCTTTC CCAGAGTCCC 
ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT TTTTTGCAGC ACCGAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG CTGCTTTCCA ACAGCAAGCT 
CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC AGGCACAACC CCCGCAGCAG 
GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC CTAATCCAAT GCCTCAAAGC 
CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG CCATGCAGAG TAACTCTCCA 
TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA ACAACAACAG 
AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG CGTCTCCAAA GCAACCACCA 
CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA ATCAGGAGCA ACAGAACCAG 
TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC AAGAGCAACA GCCCATGCAA 
TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG GTCCTACCCA GTCGGAATCA 
TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT TGGTACAAGG GTCACCTAGT 
TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT CCATGTCTGC CTTGCAGACC 
AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT CCCCTCAGAA CAACATGCCT 
GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA CTTTATTTCA CAACACAGCA 
GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT CTCAGCAGAC ATCAGGAATG 
TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CCTCTGGACC AGCTACATTG 
CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC AAAACGAGGG CCAGCCACCT 
GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CACTGGCATC CTCTATAAAC 
ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC 
TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521124.1
CDS	104..4081
Translation	MLLQLPPLPPWAVLAAPLPPLPALPFILPQSPTARLCKWRAAPQPWGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_011522822.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522822.2

ORF Insert Sequence:

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT CTAGTCCAGC AAGACCTTGC 
TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT GTAATTTAGA TAAGGTAAAT 
ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA GTATGATTAA GAGTGAAGAT 
GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA CTATTTTTAA GACTACAAAG 
TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA ACATAGCAGG AAATGGCTCT 
TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG AAAAACAGCA GCAGATTCAG 
CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA CCCAGGACAT CTCACAGCCT 
GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA ACAGCGATGC ACTATTGCAG 
CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG AGATATTACA GTCAGATGGT 
ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC AACAGCAGCA GTCACCACTA 
CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA ATATTTTTCC ATCACCAAAT 
AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG CAGGGAGTTT CACAGGCAGT 
ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC AAGTTTTAGA GGCACAGCAG 
CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG AGAATGTTCA AGAGCAGCTT 
AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG GTGTAAGCCC TGGAATGTTT 
TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT TATTACCTGG AAGAGCTGAA 
AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC AGCAGCAGCA GCAGCAGCAA 
CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA TGCAACAGAG TATCTGCCAG 
GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG CTTCAGCAAA TGGAAACCTT 
CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA GTGCATTGTC TACCAATGAG 
GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG CAGTTTCTGG AAATGAAACT 
TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA CCATGTTTCA GACATCAAGT 
TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA TTCAGAACAG TGTCTTTCAG 
ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC AAGTTAACCT TTTTTCATCC 
ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC AACAAGGTAA TGGTTTATTC 
CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT TTTTGCAGCA GTCTTCTCAT 
TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG ATGCTCAGAA CCTTTCCCAG 
GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG TCCACAGTCA GACTTCTACA 
ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC AAAGTACCAT TGCTGTGTTA 
CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA TATTTCTTTC CCAGAGTCCC 
ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT TTTTTGCAGC ACCGAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG CTGCTTTCCA ACAGCAAGCT 
CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC AGGCACAACC CCCGCAGCAG 
GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC CTAATCCAAT GCCTCAAAGC 
CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG CCATGCAGAG TAACTCTCCA 
TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA ACAACAACAG 
AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG CGTCTCCAAA GCAACCACCA 
CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA ATCAGGAGCA ACAGAACCAG 
TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC AAGAGCAACA GCCCATGCAA 
TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG GTCCTACCCA GTCGGAATCA 
TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT TGGTACAAGG GTCACCTAGT 
TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT CCATGTCTGC CTTGCAGACC 
AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT CCCCTCAGAA CAACATGCCT 
GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA CTTTATTTCA CAACACAGCA 
GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT CTCAGCAGAC ATCAGGAATG 
TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CCTCTGGACC AGCTACATTG 
CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC AAAACGAGGG CCAGCCACCT 
GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CACTGGCATC CTCTATAAAC 
ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC 
TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_017022872.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878361.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu64334
Clone ID Related Accession (Same CDS sequence)	XM_011522819.2 , XM_017022872.1 , XM_011522819.3 , XM_011522820.2 , XM_011522822.3 , XM_011522822.2 , XM_017022872.2 , XM_011522820.3
Accession Version	XM_017022872.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022872.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT CTAGTCCAGC AAGACCTTGC 
TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT GTAATTTAGA TAAGGTAAAT 
ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA GTATGATTAA GAGTGAAGAT 
GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA CTATTTTTAA GACTACAAAG 
TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA ACATAGCAGG AAATGGCTCT 
TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG AAAAACAGCA GCAGATTCAG 
CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA CCCAGGACAT CTCACAGCCT 
GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA ACAGCGATGC ACTATTGCAG 
CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG AGATATTACA GTCAGATGGT 
ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC AACAGCAGCA GTCACCACTA 
CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA ATATTTTTCC ATCACCAAAT 
AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG CAGGGAGTTT CACAGGCAGT 
ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC AAGTTTTAGA GGCACAGCAG 
CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG AGAATGTTCA AGAGCAGCTT 
AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG GTGTAAGCCC TGGAATGTTT 
TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT TATTACCTGG AAGAGCTGAA 
AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC AGCAGCAGCA GCAGCAGCAA 
CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA TGCAACAGAG TATCTGCCAG 
GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG CTTCAGCAAA TGGAAACCTT 
CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA GTGCATTGTC TACCAATGAG 
GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG CAGTTTCTGG AAATGAAACT 
TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA CCATGTTTCA GACATCAAGT 
TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA TTCAGAACAG TGTCTTTCAG 
ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC AAGTTAACCT TTTTTCATCC 
ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC AACAAGGTAA TGGTTTATTC 
CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT TTTTGCAGCA GTCTTCTCAT 
TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG ATGCTCAGAA CCTTTCCCAG 
GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG TCCACAGTCA GACTTCTACA 
ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC AAAGTACCAT TGCTGTGTTA 
CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA TATTTCTTTC CCAGAGTCCC 
ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT TTTTTGCAGC ACCGAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG CTGCTTTCCA ACAGCAAGCT 
CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC AGGCACAACC CCCGCAGCAG 
GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC CTAATCCAAT GCCTCAAAGC 
CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG CCATGCAGAG TAACTCTCCA 
TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA ACAACAACAG 
AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG CGTCTCCAAA GCAACCACCA 
CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA ATCAGGAGCA ACAGAACCAG 
TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC AAGAGCAACA GCCCATGCAA 
TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG GTCCTACCCA GTCGGAATCA 
TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT TGGTACAAGG GTCACCTAGT 
TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT CCATGTCTGC CTTGCAGACC 
AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT CCCCTCAGAA CAACATGCCT 
GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA CTTTATTTCA CAACACAGCA 
GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT CTCAGCAGAC ATCAGGAATG 
TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CCTCTGGACC AGCTACATTG 
CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC AAAACGAGGG CCAGCCACCT 
GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CACTGGCATC CTCTATAAAC 
ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC 
TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878361.1
CDS	885..4862
Translation	MLLQLPPLPPWAVLAAPLPPLPALPFILPQSPTARLCKWRAAPQPWGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_017022872.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022872.2

ORF Insert Sequence:

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT CTAGTCCAGC AAGACCTTGC 
TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT GTAATTTAGA TAAGGTAAAT 
ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA GTATGATTAA GAGTGAAGAT 
GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA CTATTTTTAA GACTACAAAG 
TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA ACATAGCAGG AAATGGCTCT 
TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG AAAAACAGCA GCAGATTCAG 
CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA CCCAGGACAT CTCACAGCCT 
GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA ACAGCGATGC ACTATTGCAG 
CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG AGATATTACA GTCAGATGGT 
ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC AACAGCAGCA GTCACCACTA 
CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA ATATTTTTCC ATCACCAAAT 
AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG CAGGGAGTTT CACAGGCAGT 
ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC AAGTTTTAGA GGCACAGCAG 
CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG AGAATGTTCA AGAGCAGCTT 
AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG GTGTAAGCCC TGGAATGTTT 
TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT TATTACCTGG AAGAGCTGAA 
AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC AGCAGCAGCA GCAGCAGCAA 
CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA TGCAACAGAG TATCTGCCAG 
GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG CTTCAGCAAA TGGAAACCTT 
CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA GTGCATTGTC TACCAATGAG 
GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG CAGTTTCTGG AAATGAAACT 
TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA CCATGTTTCA GACATCAAGT 
TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA TTCAGAACAG TGTCTTTCAG 
ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC AAGTTAACCT TTTTTCATCC 
ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC AACAAGGTAA TGGTTTATTC 
CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT TTTTGCAGCA GTCTTCTCAT 
TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG ATGCTCAGAA CCTTTCCCAG 
GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG TCCACAGTCA GACTTCTACA 
ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC AAAGTACCAT TGCTGTGTTA 
CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA TATTTCTTTC CCAGAGTCCC 
ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT TTTTTGCAGC ACCGAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG CTGCTTTCCA ACAGCAAGCT 
CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC AGGCACAACC CCCGCAGCAG 
GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC CTAATCCAAT GCCTCAAAGC 
CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG CCATGCAGAG TAACTCTCCA 
TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA ACAACAACAG 
AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG CGTCTCCAAA GCAACCACCA 
CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA ATCAGGAGCA ACAGAACCAG 
TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC AAGAGCAACA GCCCATGCAA 
TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG GTCCTACCCA GTCGGAATCA 
TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT TGGTACAAGG GTCACCTAGT 
TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT CCATGTCTGC CTTGCAGACC 
AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT CCCCTCAGAA CAACATGCCT 
GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA CTTTATTTCA CAACACAGCA 
GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT CTCAGCAGAC ATCAGGAATG 
TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CCTCTGGACC AGCTACATTG 
CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC AAAACGAGGG CCAGCCACCT 
GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CACTGGCATC CTCTATAAAC 
ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC 
TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_011522820.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521122.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X7, mRNA.

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CloneID	OHu64334
Clone ID Related Accession (Same CDS sequence)	XM_011522819.2 , XM_017022872.1 , XM_011522819.3 , XM_011522820.2 , XM_011522822.3 , XM_011522822.2 , XM_017022872.2 , XM_011522820.3
Accession Version	XM_011522820.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011522820.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT CTAGTCCAGC AAGACCTTGC 
TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT GTAATTTAGA TAAGGTAAAT 
ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA GTATGATTAA GAGTGAAGAT 
GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA CTATTTTTAA GACTACAAAG 
TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA ACATAGCAGG AAATGGCTCT 
TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG AAAAACAGCA GCAGATTCAG 
CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA CCCAGGACAT CTCACAGCCT 
GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA ACAGCGATGC ACTATTGCAG 
CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG AGATATTACA GTCAGATGGT 
ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC AACAGCAGCA GTCACCACTA 
CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA ATATTTTTCC ATCACCAAAT 
AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG CAGGGAGTTT CACAGGCAGT 
ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC AAGTTTTAGA GGCACAGCAG 
CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG AGAATGTTCA AGAGCAGCTT 
AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG GTGTAAGCCC TGGAATGTTT 
TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT TATTACCTGG AAGAGCTGAA 
AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC AGCAGCAGCA GCAGCAGCAA 
CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA TGCAACAGAG TATCTGCCAG 
GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG CTTCAGCAAA TGGAAACCTT 
CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA GTGCATTGTC TACCAATGAG 
GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG CAGTTTCTGG AAATGAAACT 
TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA CCATGTTTCA GACATCAAGT 
TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA TTCAGAACAG TGTCTTTCAG 
ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC AAGTTAACCT TTTTTCATCC 
ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC AACAAGGTAA TGGTTTATTC 
CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT TTTTGCAGCA GTCTTCTCAT 
TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG ATGCTCAGAA CCTTTCCCAG 
GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG TCCACAGTCA GACTTCTACA 
ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC AAAGTACCAT TGCTGTGTTA 
CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA TATTTCTTTC CCAGAGTCCC 
ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT TTTTTGCAGC ACCGAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG CTGCTTTCCA ACAGCAAGCT 
CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC AGGCACAACC CCCGCAGCAG 
GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC CTAATCCAAT GCCTCAAAGC 
CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG CCATGCAGAG TAACTCTCCA 
TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA ACAACAACAG 
AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG CGTCTCCAAA GCAACCACCA 
CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA ATCAGGAGCA ACAGAACCAG 
TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC AAGAGCAACA GCCCATGCAA 
TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG GTCCTACCCA GTCGGAATCA 
TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT TGGTACAAGG GTCACCTAGT 
TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT CCATGTCTGC CTTGCAGACC 
AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT CCCCTCAGAA CAACATGCCT 
GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA CTTTATTTCA CAACACAGCA 
GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT CTCAGCAGAC ATCAGGAATG 
TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CCTCTGGACC AGCTACATTG 
CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC AAAACGAGGG CCAGCCACCT 
GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CACTGGCATC CTCTATAAAC 
ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC 
TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521122.1
CDS	156..4133
Translation	MLLQLPPLPPWAVLAAPLPPLPALPFILPQSPTARLCKWRAAPQPWGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_011522820.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522820.3

ORF Insert Sequence:

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT CTAGTCCAGC AAGACCTTGC 
TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT GTAATTTAGA TAAGGTAAAT 
ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA GTATGATTAA GAGTGAAGAT 
GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA CTATTTTTAA GACTACAAAG 
TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA ACATAGCAGG AAATGGCTCT 
TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG AAAAACAGCA GCAGATTCAG 
CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA CCCAGGACAT CTCACAGCCT 
GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA ACAGCGATGC ACTATTGCAG 
CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG AGATATTACA GTCAGATGGT 
ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC AACAGCAGCA GTCACCACTA 
CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA ATATTTTTCC ATCACCAAAT 
AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG CAGGGAGTTT CACAGGCAGT 
ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC AAGTTTTAGA GGCACAGCAG 
CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG AGAATGTTCA AGAGCAGCTT 
AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG GTGTAAGCCC TGGAATGTTT 
TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT TATTACCTGG AAGAGCTGAA 
AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC AGCAGCAGCA GCAGCAGCAA 
CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA TGCAACAGAG TATCTGCCAG 
GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG CTTCAGCAAA TGGAAACCTT 
CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA GTGCATTGTC TACCAATGAG 
GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG CAGTTTCTGG AAATGAAACT 
TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA CCATGTTTCA GACATCAAGT 
TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA TTCAGAACAG TGTCTTTCAG 
ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC AAGTTAACCT TTTTTCATCC 
ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC AACAAGGTAA TGGTTTATTC 
CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT TTTTGCAGCA GTCTTCTCAT 
TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG ATGCTCAGAA CCTTTCCCAG 
GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG TCCACAGTCA GACTTCTACA 
ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC AAAGTACCAT TGCTGTGTTA 
CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA TATTTCTTTC CCAGAGTCCC 
ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT TTTTTGCAGC ACCGAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG CTGCTTTCCA ACAGCAAGCT 
CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC AGGCACAACC CCCGCAGCAG 
GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC CTAATCCAAT GCCTCAAAGC 
CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG CCATGCAGAG TAACTCTCCA 
TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA ACAACAACAG 
AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG CGTCTCCAAA GCAACCACCA 
CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA ATCAGGAGCA ACAGAACCAG 
TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC AAGAGCAACA GCCCATGCAA 
TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG GTCCTACCCA GTCGGAATCA 
TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT TGGTACAAGG GTCACCTAGT 
TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT CCATGTCTGC CTTGCAGACC 
AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT CCCCTCAGAA CAACATGCCT 
GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA CTTTATTTCA CAACACAGCA 
GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT CTCAGCAGAC ATCAGGAATG 
TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CCTCTGGACC AGCTACATTG 
CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC AAAACGAGGG CCAGCCACCT 
GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CACTGGCATC CTCTATAAAC 
ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC 
TTGACTGGCT CCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_017022871.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878360.1 Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu85814
Clone ID Related Accession (Same CDS sequence)	XM_017022871.2 , XM_017022871.1
Accession Version	XM_017022871.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022871.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCTGGTGCTT TGAATGTAAA TGTGAAGAAG 
GAAATATCTA GTCCAGCAAG ACCTTGCTCT TTTGAAGAGG CCATGAAAGC AATGAAAACT 
ACTGGATGTA ATTTAGATAA GGTAAATATT ATCCCTAATG CCCTGATGAC TCCACTCATA 
CCAAGCAGTA TGATTAAGAG TGAAGATGTT ACTCCAATGG AAGTAACAGC AGAAAAAAGA 
TCTTCCACTA TTTTTAAGAC TACAAAGTCT GTTGGATCAA CTCAGCAAAC ATTAGAAAAC 
ATCTCAAACA TAGCAGGAAA TGGCTCTTTT TCATCACCAT CATCTTCCCA CCTACCTTCT 
GAAAATGAAA AACAGCAGCA GATTCAGCCC AAGGCATACA ACCCAGAGAC CCTGACAACT 
ATTCAAACCC AGGACATCTC ACAGCCTGGT ACTTTTCCAG CAGTTTCTGC TTCTAGTCAG 
CTGCCCAACA GCGATGCACT ATTGCAGCAG GCTACACAGT TTCAGACAAG AGAAACTCAG 
TCTAGAGAGA TATTACAGTC AGATGGTACA GTGGTTAATT TGTCACAACT GACTGAGGCA 
TCACAACAAC AGCAGCAGTC ACCACTACAA GAACAAGCAC AGACTTTACA GCAGCAGATT 
TCATCAAATA TTTTTCCATC ACCAAATAGT GTGAGTCAGC TTCAGAATAC TATTCAGCAG 
CTGCAAGCAG GGAGTTTCAC AGGCAGTACT GCTAGTGGCA GCAGTGGAAG TGTTGACTTG 
GTCCAACAAG TTTTAGAGGC ACAGCAGCAG TTATCTTCAG TTTTATTTTC TGCTCCAGAT 
GGTAATGAGA ATGTTCAAGA GCAGCTTAGT GCAGATATTT TTCAACAAGT CAGTCAAATT 
CAGAGTGGTG TAAGCCCTGG AATGTTTTCC TCAACAGAGC CAACAGTCCA TACCAGACCA 
GATAATTTAT TACCTGGAAG AGCTGAAAGT GTTCATCCAC AGTCTGAAAA CACGTTATCT 
AATCAACAGC AGCAGCAGCA GCAGCAACAG CAAGTGATGG AATCTTCAGC CGCAATGGTG 
ATGGAGATGC AACAGAGTAT CTGCCAGGCA GCTGCCCAGA TTCAGTCAGA GTTATTCCCT 
TCAACTGCTT CAGCAAATGG AAACCTTCAG CAATCGCCAG TTTACCAGCA GACTTCTCAC 
ATGATGAGTG CATTGTCTAC CAATGAGGAT ATGCAAATGC AGTGTGAATT GTTTTCTTCT 
CCTCCTGCAG TTTCTGGAAA TGAAACTTCT ACAACTACCA CACAGCAGGT TGCAACCCCT 
GGCACTACCA TGTTTCAGAC ATCAAGTTCA GGAGATGGAG AAGAAACTGG AACACAAGCA 
AAACAGATTC AGAACAGTGT CTTTCAGACC ATGGTCCAAA TGCAACATAG TGGGGACAAT 
CAACCTCAAG TTAACCTTTT TTCATCCACA AAAAGTATGA TGAGTGTTCA GAATAGTGGT 
ACCCAACAAC AAGGTAATGG TTTATTCCAG CAAGGGAATG AGATGATGTC ACTTCAATCT 
GGAAATTTTT TGCAGCAGTC TTCTCATTCA CAGGCCCAAC TTTTTCATCC TCAAAATCCT 
ATTGCCGATG CTCAGAACCT TTCCCAGGAA ACTCAAGGTT CTCTCTTTCA TAGTCCAAAT 
CCTATTGTCC ACAGTCAGAC TTCTACAACC TCCTCTGAAC AAATGCAGCC TCCAATGTTT 
CACTCTCAAA GTACCATTGC TGTGTTACAG GGCTCTTCAG TTCCTCAAGA CCAGCAGTCA 
ACCAACATAT TTCTTTCCCA GAGTCCCATG AATAATCTTC AGACTAACAC AGTAGCCCAA 
GAAGCATTTT TTGCAGCACC GAACTCAATT TCTCCACTTC AGTCAACATC AAACAGTGAA 
CAACAAGCTG CTTTCCAACA GCAAGCTCCA ATATCACACA TCCAGACTCC TATGCTTTCC 
CAAGAACAGG CACAACCCCC GCAGCAGGGT TTATTTCAGC CTCAGGTGGC CCTGGGCTCC 
CTTCCACCTA ATCCAATGCC TCAAAGCCAA CAAGGAACCA TGTTCCAGTC ACAGCACTCA 
ATAGTTGCCA TGCAGAGTAA CTCTCCATCC CAGGAACAGC AGCAGCAGCA GCAACAGCAG 
CAGCAACAGC AGCAGCAACA ACAACAGAGC ATTTTATTCA GTAATCAGAA TACCATGGCT 
ACAATGGCGT CTCCAAAGCA ACCACCACCA AACATGATAT TCAACCCAAA TCAAAATCCA 
ATGGCTAATC AGGAGCAACA GAACCAGTCA ATTTTTCACC AACAAAGTAA CATGGCCCCA 
ATGAATCAAG AGCAACAGCC CATGCAATTT CAGAGTCAGT CCACAGTTTC CTCACTTCAG 
AACCCAGGTC CTACCCAGTC GGAATCATCA CAGACCCCCT TGTTCCATAG CTCTCCTCAG 
ATTCAGTTGG TACAAGGGTC ACCTAGTTCT CAAGAGCAGC AAGTAACTCT CTTCTTATCT 
CCAGCATCCA TGTCTGCCTT GCAGACCAGT ATAAATCAAC AAGATATGCA ACAGTCTCCT 
CTTTATTCCC CTCAGAACAA CATGCCTGGA ATTCAAGGAG CCACATCTTC GCCTCAACCA 
CAGGCTACTT TATTTCACAA CACAGCAGGA GGCACAATGA ACCAACTGCA GAATTCTCCT 
GGCTCATCTC AGCAGACATC AGGAATGTTC TTATTTGGCA TTCAAAATAA CTGTAGTCAG 
CTTTTAACCT CTGGACCAGC TACATTGCCT GATCAGTTGA TGGCCATAAG TCAGCCAGGC 
CAACCACAAA ACGAGGGCCA GCCACCTGTG ACAACACTTC TTTCTCAGCA AATGCCAGAG 
AATTCTCCAC TGGCATCCTC TATAAACACC AACCAGAACA TCGAAAAGAT TGATTTGCTT 
GTTTCATTGC AAAACCAAGG GAACAACTTG ACTGGCTCCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878360.1
CDS	640..5004
Translation	MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_017022871.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T cells 5 (NFAT5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022871.2

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCTGGTGCTT TGAATGTAAA TGTGAAGAAG 
GAAATATCTA GTCCAGCAAG ACCTTGCTCT TTTGAAGAGG CCATGAAAGC AATGAAAACT 
ACTGGATGTA ATTTAGATAA GGTAAATATT ATCCCTAATG CCCTGATGAC TCCACTCATA 
CCAAGCAGTA TGATTAAGAG TGAAGATGTT ACTCCAATGG AAGTAACAGC AGAAAAAAGA 
TCTTCCACTA TTTTTAAGAC TACAAAGTCT GTTGGATCAA CTCAGCAAAC ATTAGAAAAC 
ATCTCAAACA TAGCAGGAAA TGGCTCTTTT TCATCACCAT CATCTTCCCA CCTACCTTCT 
GAAAATGAAA AACAGCAGCA GATTCAGCCC AAGGCATACA ACCCAGAGAC CCTGACAACT 
ATTCAAACCC AGGACATCTC ACAGCCTGGT ACTTTTCCAG CAGTTTCTGC TTCTAGTCAG 
CTGCCCAACA GCGATGCACT ATTGCAGCAG GCTACACAGT TTCAGACAAG AGAAACTCAG 
TCTAGAGAGA TATTACAGTC AGATGGTACA GTGGTTAATT TGTCACAACT GACTGAGGCA 
TCACAACAAC AGCAGCAGTC ACCACTACAA GAACAAGCAC AGACTTTACA GCAGCAGATT 
TCATCAAATA TTTTTCCATC ACCAAATAGT GTGAGTCAGC TTCAGAATAC TATTCAGCAG 
CTGCAAGCAG GGAGTTTCAC AGGCAGTACT GCTAGTGGCA GCAGTGGAAG TGTTGACTTG 
GTCCAACAAG TTTTAGAGGC ACAGCAGCAG TTATCTTCAG TTTTATTTTC TGCTCCAGAT 
GGTAATGAGA ATGTTCAAGA GCAGCTTAGT GCAGATATTT TTCAACAAGT CAGTCAAATT 
CAGAGTGGTG TAAGCCCTGG AATGTTTTCC TCAACAGAGC CAACAGTCCA TACCAGACCA 
GATAATTTAT TACCTGGAAG AGCTGAAAGT GTTCATCCAC AGTCTGAAAA CACGTTATCT 
AATCAACAGC AGCAGCAGCA GCAGCAACAG CAAGTGATGG AATCTTCAGC CGCAATGGTG 
ATGGAGATGC AACAGAGTAT CTGCCAGGCA GCTGCCCAGA TTCAGTCAGA GTTATTCCCT 
TCAACTGCTT CAGCAAATGG AAACCTTCAG CAATCGCCAG TTTACCAGCA GACTTCTCAC 
ATGATGAGTG CATTGTCTAC CAATGAGGAT ATGCAAATGC AGTGTGAATT GTTTTCTTCT 
CCTCCTGCAG TTTCTGGAAA TGAAACTTCT ACAACTACCA CACAGCAGGT TGCAACCCCT 
GGCACTACCA TGTTTCAGAC ATCAAGTTCA GGAGATGGAG AAGAAACTGG AACACAAGCA 
AAACAGATTC AGAACAGTGT CTTTCAGACC ATGGTCCAAA TGCAACATAG TGGGGACAAT 
CAACCTCAAG TTAACCTTTT TTCATCCACA AAAAGTATGA TGAGTGTTCA GAATAGTGGT 
ACCCAACAAC AAGGTAATGG TTTATTCCAG CAAGGGAATG AGATGATGTC ACTTCAATCT 
GGAAATTTTT TGCAGCAGTC TTCTCATTCA CAGGCCCAAC TTTTTCATCC TCAAAATCCT 
ATTGCCGATG CTCAGAACCT TTCCCAGGAA ACTCAAGGTT CTCTCTTTCA TAGTCCAAAT 
CCTATTGTCC ACAGTCAGAC TTCTACAACC TCCTCTGAAC AAATGCAGCC TCCAATGTTT 
CACTCTCAAA GTACCATTGC TGTGTTACAG GGCTCTTCAG TTCCTCAAGA CCAGCAGTCA 
ACCAACATAT TTCTTTCCCA GAGTCCCATG AATAATCTTC AGACTAACAC AGTAGCCCAA 
GAAGCATTTT TTGCAGCACC GAACTCAATT TCTCCACTTC AGTCAACATC AAACAGTGAA 
CAACAAGCTG CTTTCCAACA GCAAGCTCCA ATATCACACA TCCAGACTCC TATGCTTTCC 
CAAGAACAGG CACAACCCCC GCAGCAGGGT TTATTTCAGC CTCAGGTGGC CCTGGGCTCC 
CTTCCACCTA ATCCAATGCC TCAAAGCCAA CAAGGAACCA TGTTCCAGTC ACAGCACTCA 
ATAGTTGCCA TGCAGAGTAA CTCTCCATCC CAGGAACAGC AGCAGCAGCA GCAACAGCAG 
CAGCAACAGC AGCAGCAACA ACAACAGAGC ATTTTATTCA GTAATCAGAA TACCATGGCT 
ACAATGGCGT CTCCAAAGCA ACCACCACCA AACATGATAT TCAACCCAAA TCAAAATCCA 
ATGGCTAATC AGGAGCAACA GAACCAGTCA ATTTTTCACC AACAAAGTAA CATGGCCCCA 
ATGAATCAAG AGCAACAGCC CATGCAATTT CAGAGTCAGT CCACAGTTTC CTCACTTCAG 
AACCCAGGTC CTACCCAGTC GGAATCATCA CAGACCCCCT TGTTCCATAG CTCTCCTCAG 
ATTCAGTTGG TACAAGGGTC ACCTAGTTCT CAAGAGCAGC AAGTAACTCT CTTCTTATCT 
CCAGCATCCA TGTCTGCCTT GCAGACCAGT ATAAATCAAC AAGATATGCA ACAGTCTCCT 
CTTTATTCCC CTCAGAACAA CATGCCTGGA ATTCAAGGAG CCACATCTTC GCCTCAACCA 
CAGGCTACTT TATTTCACAA CACAGCAGGA GGCACAATGA ACCAACTGCA GAATTCTCCT 
GGCTCATCTC AGCAGACATC AGGAATGTTC TTATTTGGCA TTCAAAATAA CTGTAGTCAG 
CTTTTAACCT CTGGACCAGC TACATTGCCT GATCAGTTGA TGGCCATAAG TCAGCCAGGC 
CAACCACAAA ACGAGGGCCA GCCACCTGTG ACAACACTTC TTTCTCAGCA AATGCCAGAG 
AATTCTCCAC TGGCATCCTC TATAAACACC AACCAGAACA TCGAAAAGAT TGATTTGCTT 
GTTTCATTGC AAAACCAAGG GAACAACTTG ACTGGCTCCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_017022871.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878360.1 Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu85814
Clone ID Related Accession (Same CDS sequence)	XM_017022871.2 , XM_017022871.1
Accession Version	XM_017022871.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCTGGTGCTT TGAATGTAAA TGTGAAGAAG 
GAAATATCTA GTCCAGCAAG ACCTTGCTCT TTTGAAGAGG CCATGAAAGC AATGAAAACT 
ACTGGATGTA ATTTAGATAA GGTAAATATT ATCCCTAATG CCCTGATGAC TCCACTCATA 
CCAAGCAGTA TGATTAAGAG TGAAGATGTT ACTCCAATGG AAGTAACAGC AGAAAAAAGA 
TCTTCCACTA TTTTTAAGAC TACAAAGTCT GTTGGATCAA CTCAGCAAAC ATTAGAAAAC 
ATCTCAAACA TAGCAGGAAA TGGCTCTTTT TCATCACCAT CATCTTCCCA CCTACCTTCT 
GAAAATGAAA AACAGCAGCA GATTCAGCCC AAGGCATACA ACCCAGAGAC CCTGACAACT 
ATTCAAACCC AGGACATCTC ACAGCCTGGT ACTTTTCCAG CAGTTTCTGC TTCTAGTCAG 
CTGCCCAACA GCGATGCACT ATTGCAGCAG GCTACACAGT TTCAGACAAG AGAAACTCAG 
TCTAGAGAGA TATTACAGTC AGATGGTACA GTGGTTAATT TGTCACAACT GACTGAGGCA 
TCACAACAAC AGCAGCAGTC ACCACTACAA GAACAAGCAC AGACTTTACA GCAGCAGATT 
TCATCAAATA TTTTTCCATC ACCAAATAGT GTGAGTCAGC TTCAGAATAC TATTCAGCAG 
CTGCAAGCAG GGAGTTTCAC AGGCAGTACT GCTAGTGGCA GCAGTGGAAG TGTTGACTTG 
GTCCAACAAG TTTTAGAGGC ACAGCAGCAG TTATCTTCAG TTTTATTTTC TGCTCCAGAT 
GGTAATGAGA ATGTTCAAGA GCAGCTTAGT GCAGATATTT TTCAACAAGT CAGTCAAATT 
CAGAGTGGTG TAAGCCCTGG AATGTTTTCC TCAACAGAGC CAACAGTCCA TACCAGACCA 
GATAATTTAT TACCTGGAAG AGCTGAAAGT GTTCATCCAC AGTCTGAAAA CACGTTATCT 
AATCAACAGC AGCAGCAGCA GCAGCAACAG CAAGTGATGG AATCTTCAGC CGCAATGGTG 
ATGGAGATGC AACAGAGTAT CTGCCAGGCA GCTGCCCAGA TTCAGTCAGA GTTATTCCCT 
TCAACTGCTT CAGCAAATGG AAACCTTCAG CAATCGCCAG TTTACCAGCA GACTTCTCAC 
ATGATGAGTG CATTGTCTAC CAATGAGGAT ATGCAAATGC AGTGTGAATT GTTTTCTTCT 
CCTCCTGCAG TTTCTGGAAA TGAAACTTCT ACAACTACCA CACAGCAGGT TGCAACCCCT 
GGCACTACCA TGTTTCAGAC ATCAAGTTCA GGAGATGGAG AAGAAACTGG AACACAAGCA 
AAACAGATTC AGAACAGTGT CTTTCAGACC ATGGTCCAAA TGCAACATAG TGGGGACAAT 
CAACCTCAAG TTAACCTTTT TTCATCCACA AAAAGTATGA TGAGTGTTCA GAATAGTGGT 
ACCCAACAAC AAGGTAATGG TTTATTCCAG CAAGGGAATG AGATGATGTC ACTTCAATCT 
GGAAATTTTT TGCAGCAGTC TTCTCATTCA CAGGCCCAAC TTTTTCATCC TCAAAATCCT 
ATTGCCGATG CTCAGAACCT TTCCCAGGAA ACTCAAGGTT CTCTCTTTCA TAGTCCAAAT 
CCTATTGTCC ACAGTCAGAC TTCTACAACC TCCTCTGAAC AAATGCAGCC TCCAATGTTT 
CACTCTCAAA GTACCATTGC TGTGTTACAG GGCTCTTCAG TTCCTCAAGA CCAGCAGTCA 
ACCAACATAT TTCTTTCCCA GAGTCCCATG AATAATCTTC AGACTAACAC AGTAGCCCAA 
GAAGCATTTT TTGCAGCACC GAACTCAATT TCTCCACTTC AGTCAACATC AAACAGTGAA 
CAACAAGCTG CTTTCCAACA GCAAGCTCCA ATATCACACA TCCAGACTCC TATGCTTTCC 
CAAGAACAGG CACAACCCCC GCAGCAGGGT TTATTTCAGC CTCAGGTGGC CCTGGGCTCC 
CTTCCACCTA ATCCAATGCC TCAAAGCCAA CAAGGAACCA TGTTCCAGTC ACAGCACTCA 
ATAGTTGCCA TGCAGAGTAA CTCTCCATCC CAGGAACAGC AGCAGCAGCA GCAACAGCAG 
CAGCAACAGC AGCAGCAACA ACAACAGAGC ATTTTATTCA GTAATCAGAA TACCATGGCT 
ACAATGGCGT CTCCAAAGCA ACCACCACCA AACATGATAT TCAACCCAAA TCAAAATCCA 
ATGGCTAATC AGGAGCAACA GAACCAGTCA ATTTTTCACC AACAAAGTAA CATGGCCCCA 
ATGAATCAAG AGCAACAGCC CATGCAATTT CAGAGTCAGT CCACAGTTTC CTCACTTCAG 
AACCCAGGTC CTACCCAGTC GGAATCATCA CAGACCCCCT TGTTCCATAG CTCTCCTCAG 
ATTCAGTTGG TACAAGGGTC ACCTAGTTCT CAAGAGCAGC AAGTAACTCT CTTCTTATCT 
CCAGCATCCA TGTCTGCCTT GCAGACCAGT ATAAATCAAC AAGATATGCA ACAGTCTCCT 
CTTTATTCCC CTCAGAACAA CATGCCTGGA ATTCAAGGAG CCACATCTTC GCCTCAACCA 
CAGGCTACTT TATTTCACAA CACAGCAGGA GGCACAATGA ACCAACTGCA GAATTCTCCT 
GGCTCATCTC AGCAGACATC AGGAATGTTC TTATTTGGCA TTCAAAATAA CTGTAGTCAG 
CTTTTAACCT CTGGACCAGC TACATTGCCT GATCAGTTGA TGGCCATAAG TCAGCCAGGC 
CAACCACAAA ACGAGGGCCA GCCACCTGTG ACAACACTTC TTTCTCAGCA AATGCCAGAG 
AATTCTCCAC TGGCATCCTC TATAAACACC AACCAGAACA TCGAAAAGAT TGATTTGCTT 
GTTTCATTGC AAAACCAAGG GAACAACTTG ACTGGCTCCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878360.1
CDS	641..5005
Translation	MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_017022871.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022871.1

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA 
AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA 
AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC 
CTGACTGAGG GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT 
TGCAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC 
AACATGACAC TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT 
TTGTGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG 
GAACTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA 
ACTTTGCCTG TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT 
CAACCATTCA CTTACACTCC AGACCCAGCA GCTGGTGCTT TGAATGTAAA TGTGAAGAAG 
GAAATATCTA GTCCAGCAAG ACCTTGCTCT TTTGAAGAGG CCATGAAAGC AATGAAAACT 
ACTGGATGTA ATTTAGATAA GGTAAATATT ATCCCTAATG CCCTGATGAC TCCACTCATA 
CCAAGCAGTA TGATTAAGAG TGAAGATGTT ACTCCAATGG AAGTAACAGC AGAAAAAAGA 
TCTTCCACTA TTTTTAAGAC TACAAAGTCT GTTGGATCAA CTCAGCAAAC ATTAGAAAAC 
ATCTCAAACA TAGCAGGAAA TGGCTCTTTT TCATCACCAT CATCTTCCCA CCTACCTTCT 
GAAAATGAAA AACAGCAGCA GATTCAGCCC AAGGCATACA ACCCAGAGAC CCTGACAACT 
ATTCAAACCC AGGACATCTC ACAGCCTGGT ACTTTTCCAG CAGTTTCTGC TTCTAGTCAG 
CTGCCCAACA GCGATGCACT ATTGCAGCAG GCTACACAGT TTCAGACAAG AGAAACTCAG 
TCTAGAGAGA TATTACAGTC AGATGGTACA GTGGTTAATT TGTCACAACT GACTGAGGCA 
TCACAACAAC AGCAGCAGTC ACCACTACAA GAACAAGCAC AGACTTTACA GCAGCAGATT 
TCATCAAATA TTTTTCCATC ACCAAATAGT GTGAGTCAGC TTCAGAATAC TATTCAGCAG 
CTGCAAGCAG GGAGTTTCAC AGGCAGTACT GCTAGTGGCA GCAGTGGAAG TGTTGACTTG 
GTCCAACAAG TTTTAGAGGC ACAGCAGCAG TTATCTTCAG TTTTATTTTC TGCTCCAGAT 
GGTAATGAGA ATGTTCAAGA GCAGCTTAGT GCAGATATTT TTCAACAAGT CAGTCAAATT 
CAGAGTGGTG TAAGCCCTGG AATGTTTTCC TCAACAGAGC CAACAGTCCA TACCAGACCA 
GATAATTTAT TACCTGGAAG AGCTGAAAGT GTTCATCCAC AGTCTGAAAA CACGTTATCT 
AATCAACAGC AGCAGCAGCA GCAGCAACAG CAAGTGATGG AATCTTCAGC CGCAATGGTG 
ATGGAGATGC AACAGAGTAT CTGCCAGGCA GCTGCCCAGA TTCAGTCAGA GTTATTCCCT 
TCAACTGCTT CAGCAAATGG AAACCTTCAG CAATCGCCAG TTTACCAGCA GACTTCTCAC 
ATGATGAGTG CATTGTCTAC CAATGAGGAT ATGCAAATGC AGTGTGAATT GTTTTCTTCT 
CCTCCTGCAG TTTCTGGAAA TGAAACTTCT ACAACTACCA CACAGCAGGT TGCAACCCCT 
GGCACTACCA TGTTTCAGAC ATCAAGTTCA GGAGATGGAG AAGAAACTGG AACACAAGCA 
AAACAGATTC AGAACAGTGT CTTTCAGACC ATGGTCCAAA TGCAACATAG TGGGGACAAT 
CAACCTCAAG TTAACCTTTT TTCATCCACA AAAAGTATGA TGAGTGTTCA GAATAGTGGT 
ACCCAACAAC AAGGTAATGG TTTATTCCAG CAAGGGAATG AGATGATGTC ACTTCAATCT 
GGAAATTTTT TGCAGCAGTC TTCTCATTCA CAGGCCCAAC TTTTTCATCC TCAAAATCCT 
ATTGCCGATG CTCAGAACCT TTCCCAGGAA ACTCAAGGTT CTCTCTTTCA TAGTCCAAAT 
CCTATTGTCC ACAGTCAGAC TTCTACAACC TCCTCTGAAC AAATGCAGCC TCCAATGTTT 
CACTCTCAAA GTACCATTGC TGTGTTACAG GGCTCTTCAG TTCCTCAAGA CCAGCAGTCA 
ACCAACATAT TTCTTTCCCA GAGTCCCATG AATAATCTTC AGACTAACAC AGTAGCCCAA 
GAAGCATTTT TTGCAGCACC GAACTCAATT TCTCCACTTC AGTCAACATC AAACAGTGAA 
CAACAAGCTG CTTTCCAACA GCAAGCTCCA ATATCACACA TCCAGACTCC TATGCTTTCC 
CAAGAACAGG CACAACCCCC GCAGCAGGGT TTATTTCAGC CTCAGGTGGC CCTGGGCTCC 
CTTCCACCTA ATCCAATGCC TCAAAGCCAA CAAGGAACCA TGTTCCAGTC ACAGCACTCA 
ATAGTTGCCA TGCAGAGTAA CTCTCCATCC CAGGAACAGC AGCAGCAGCA GCAACAGCAG 
CAGCAACAGC AGCAGCAACA ACAACAGAGC ATTTTATTCA GTAATCAGAA TACCATGGCT 
ACAATGGCGT CTCCAAAGCA ACCACCACCA AACATGATAT TCAACCCAAA TCAAAATCCA 
ATGGCTAATC AGGAGCAACA GAACCAGTCA ATTTTTCACC AACAAAGTAA CATGGCCCCA 
ATGAATCAAG AGCAACAGCC CATGCAATTT CAGAGTCAGT CCACAGTTTC CTCACTTCAG 
AACCCAGGTC CTACCCAGTC GGAATCATCA CAGACCCCCT TGTTCCATAG CTCTCCTCAG 
ATTCAGTTGG TACAAGGGTC ACCTAGTTCT CAAGAGCAGC AAGTAACTCT CTTCTTATCT 
CCAGCATCCA TGTCTGCCTT GCAGACCAGT ATAAATCAAC AAGATATGCA ACAGTCTCCT 
CTTTATTCCC CTCAGAACAA CATGCCTGGA ATTCAAGGAG CCACATCTTC GCCTCAACCA 
CAGGCTACTT TATTTCACAA CACAGCAGGA GGCACAATGA ACCAACTGCA GAATTCTCCT 
GGCTCATCTC AGCAGACATC AGGAATGTTC TTATTTGGCA TTCAAAATAA CTGTAGTCAG 
CTTTTAACCT CTGGACCAGC TACATTGCCT GATCAGTTGA TGGCCATAAG TCAGCCAGGC 
CAACCACAAA ACGAGGGCCA GCCACCTGTG ACAACACTTC TTTCTCAGCA AATGCCAGAG 
AATTCTCCAC TGGCATCCTC TATAAACACC AACCAGAACA TCGAAAAGAT TGATTTGCTT 
GTTTCATTGC AAAACCAAGG GAACAACTTG ACTGGCTCCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_017022873.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878362.1 Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X10, mRNA.

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CloneID	OHu85815
Clone ID Related Accession (Same CDS sequence)	NM_001367709.1 , XM_017022873.1
Accession Version	XM_017022873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear factor of activated T-cells 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCTGGTGCTT TGAATGTAAA TGTGAAGAAG GAAATATCTA GTCCAGCAAG ACCTTGCTCT 
TTTGAAGAGG CCATGAAAGC AATGAAAACT ACTGGATGTA ATTTAGATAA GGTAAATATT 
ATCCCTAATG CCCTGATGAC TCCACTCATA CCAAGCAGTA TGATTAAGAG TGAAGATGTT 
ACTCCAATGG AAGTAACAGC AGAAAAAAGA TCTTCCACTA TTTTTAAGAC TACAAAGTCT 
GTTGGATCAA CTCAGCAAAC ATTAGAAAAC ATCTCAAACA TAGCAGGAAA TGGCTCTTTT 
TCATCACCAT CATCTTCCCA CCTACCTTCT GAAAATGAAA AACAGCAGCA GATTCAGCCC 
AAGGCATACA ACCCAGAGAC CCTGACAACT ATTCAAACCC AGGACATCTC ACAGCCTGGT 
ACTTTTCCAG CAGTTTCTGC TTCTAGTCAG CTGCCCAACA GCGATGCACT ATTGCAGCAG 
GCTACACAGT TTCAGACAAG AGAAACTCAG TCTAGAGAGA TATTACAGTC AGATGGTACA 
GTGGTTAATT TGTCACAACT GACTGAGGCA TCACAACAAC AGCAGCAGTC ACCACTACAA 
GAACAAGCAC AGACTTTACA GCAGCAGATT TCATCAAATA TTTTTCCATC ACCAAATAGT 
GTGAGTCAGC TTCAGAATAC TATTCAGCAG CTGCAAGCAG GGAGTTTCAC AGGCAGTACT 
GCTAGTGGCA GCAGTGGAAG TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAGCAGCAG 
TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAATGAGA ATGTTCAAGA GCAGCTTAGT 
GCAGATATTT TTCAACAAGT CAGTCAAATT CAGAGTGGTG TAAGCCCTGG AATGTTTTCC 
TCAACAGAGC CAACAGTCCA TACCAGACCA GATAATTTAT TACCTGGAAG AGCTGAAAGT 
GTTCATCCAC AGTCTGAAAA CACGTTATCT AATCAACAGC AGCAGCAGCA GCAGCAACAG 
CAAGTGATGG AATCTTCAGC CGCAATGGTG ATGGAGATGC AACAGAGTAT CTGCCAGGCA 
GCTGCCCAGA TTCAGTCAGA GTTATTCCCT TCAACTGCTT CAGCAAATGG AAACCTTCAG 
CAATCGCCAG TTTACCAGCA GACTTCTCAC ATGATGAGTG CATTGTCTAC CAATGAGGAT 
ATGCAAATGC AGTGTGAATT GTTTTCTTCT CCTCCTGCAG TTTCTGGAAA TGAAACTTCT 
ACAACTACCA CACAGCAGGT TGCAACCCCT GGCACTACCA TGTTTCAGAC ATCAAGTTCA 
GGAGATGGAG AAGAAACTGG AACACAAGCA AAACAGATTC AGAACAGTGT CTTTCAGACC 
ATGGTCCAAA TGCAACATAG TGGGGACAAT CAACCTCAAG TTAACCTTTT TTCATCCACA 
AAAAGTATGA TGAGTGTTCA GAATAGTGGT ACCCAACAAC AAGGTAATGG TTTATTCCAG 
CAAGGGAATG AGATGATGTC ACTTCAATCT GGAAATTTTT TGCAGCAGTC TTCTCATTCA 
CAGGCCCAAC TTTTTCATCC TCAAAATCCT ATTGCCGATG CTCAGAACCT TTCCCAGGAA 
ACTCAAGGTT CTCTCTTTCA TAGTCCAAAT CCTATTGTCC ACAGTCAGAC TTCTACAACC 
TCCTCTGAAC AAATGCAGCC TCCAATGTTT CACTCTCAAA GTACCATTGC TGTGTTACAG 
GGCTCTTCAG TTCCTCAAGA CCAGCAGTCA ACCAACATAT TTCTTTCCCA GAGTCCCATG 
AATAATCTTC AGACTAACAC AGTAGCCCAA GAAGCATTTT TTGCAGCACC GAACTCAATT 
TCTCCACTTC AGTCAACATC AAACAGTGAA CAACAAGCTG CTTTCCAACA GCAAGCTCCA 
ATATCACACA TCCAGACTCC TATGCTTTCC CAAGAACAGG CACAACCCCC GCAGCAGGGT 
TTATTTCAGC CTCAGGTGGC CCTGGGCTCC CTTCCACCTA ATCCAATGCC TCAAAGCCAA 
CAAGGAACCA TGTTCCAGTC ACAGCACTCA ATAGTTGCCA TGCAGAGTAA CTCTCCATCC 
CAGGAACAGC AGCAGCAGCA GCAACAGCAG CAGCAACAGC AGCAGCAACA ACAACAGAGC 
ATTTTATTCA GTAATCAGAA TACCATGGCT ACAATGGCGT CTCCAAAGCA ACCACCACCA 
AACATGATAT TCAACCCAAA TCAAAATCCA ATGGCTAATC AGGAGCAACA GAACCAGTCA 
ATTTTTCACC AACAAAGTAA CATGGCCCCA ATGAATCAAG AGCAACAGCC CATGCAATTT 
CAGAGTCAGT CCACAGTTTC CTCACTTCAG AACCCAGGTC CTACCCAGTC GGAATCATCA 
CAGACCCCCT TGTTCCATAG CTCTCCTCAG ATTCAGTTGG TACAAGGGTC ACCTAGTTCT 
CAAGAGCAGC AAGTAACTCT CTTCTTATCT CCAGCATCCA TGTCTGCCTT GCAGACCAGT 
ATAAATCAAC AAGATATGCA ACAGTCTCCT CTTTATTCCC CTCAGAACAA CATGCCTGGA 
ATTCAAGGAG CCACATCTTC GCCTCAACCA CAGGCTACTT TATTTCACAA CACAGCAGGA 
GGCACAATGA ACCAACTGCA GAATTCTCCT GGCTCATCTC AGCAGACATC AGGAATGTTC 
TTATTTGGCA TTCAAAATAA CTGTAGTCAG CTTTTAACCT CTGGACCAGC TACATTGCCT 
GATCAGTTGA TGGCCATAAG TCAGCCAGGC CAACCACAAA ACGAGGGCCA GCCACCTGTG 
ACAACACTTC TTTCTCAGCA AATGCCAGAG AATTCTCCAC TGGCATCCTC TATAAACACC 
AACCAGAACA TCGAAAAGAT TGATTTGCTT GTTTCATTGC AAAACCAAGG GAACAACTTG 
ACTGGCTCCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878362.1
CDS	497..4471
Translation	MLLQLPPLPPWAVLAAPLPPLPALPFILPQSPTARLCKWRAAPQPWGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Target ORF information:

RefSeq Version	XM_017022873.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022873.1

ORF Insert Sequence:

ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTGGAGA 
GCTGCTCCTC AGCCGTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC 
CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA 
CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG GCAGCCGTGG CTCAGTGAAA 
GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG GCCATAATGA ACCTGTAGTG 
TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC CACATGGATT TTATCAGGCC 
TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG TGGACATTGA AGGCACTACT 
GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC TGGCGGTGGA CTGCGTAGGG 
ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAG 
AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA TGAGGAAAGA TGGCTCCACT 
TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC AGCCAGCAGG AGTGCCAGAA 
ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG AAGAAGAAGT GTTTTTAATC 
GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC 
TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC ATCAGAATCA TCTTATTGTG 
AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG TGTCAGTGGG AATATATGTA 
GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA CTTACACTCC AGACCCAGCA 
GCTGGTGCTT TGAATGTAAA TGTGAAGAAG GAAATATCTA GTCCAGCAAG ACCTTGCTCT 
TTTGAAGAGG CCATGAAAGC AATGAAAACT ACTGGATGTA ATTTAGATAA GGTAAATATT 
ATCCCTAATG CCCTGATGAC TCCACTCATA CCAAGCAGTA TGATTAAGAG TGAAGATGTT 
ACTCCAATGG AAGTAACAGC AGAAAAAAGA TCTTCCACTA TTTTTAAGAC TACAAAGTCT 
GTTGGATCAA CTCAGCAAAC ATTAGAAAAC ATCTCAAACA TAGCAGGAAA TGGCTCTTTT 
TCATCACCAT CATCTTCCCA CCTACCTTCT GAAAATGAAA AACAGCAGCA GATTCAGCCC 
AAGGCATACA ACCCAGAGAC CCTGACAACT ATTCAAACCC AGGACATCTC ACAGCCTGGT 
ACTTTTCCAG CAGTTTCTGC TTCTAGTCAG CTGCCCAACA GCGATGCACT ATTGCAGCAG 
GCTACACAGT TTCAGACAAG AGAAACTCAG TCTAGAGAGA TATTACAGTC AGATGGTACA 
GTGGTTAATT TGTCACAACT GACTGAGGCA TCACAACAAC AGCAGCAGTC ACCACTACAA 
GAACAAGCAC AGACTTTACA GCAGCAGATT TCATCAAATA TTTTTCCATC ACCAAATAGT 
GTGAGTCAGC TTCAGAATAC TATTCAGCAG CTGCAAGCAG GGAGTTTCAC AGGCAGTACT 
GCTAGTGGCA GCAGTGGAAG TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAGCAGCAG 
TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAATGAGA ATGTTCAAGA GCAGCTTAGT 
GCAGATATTT TTCAACAAGT CAGTCAAATT CAGAGTGGTG TAAGCCCTGG AATGTTTTCC 
TCAACAGAGC CAACAGTCCA TACCAGACCA GATAATTTAT TACCTGGAAG AGCTGAAAGT 
GTTCATCCAC AGTCTGAAAA CACGTTATCT AATCAACAGC AGCAGCAGCA GCAGCAACAG 
CAAGTGATGG AATCTTCAGC CGCAATGGTG ATGGAGATGC AACAGAGTAT CTGCCAGGCA 
GCTGCCCAGA TTCAGTCAGA GTTATTCCCT TCAACTGCTT CAGCAAATGG AAACCTTCAG 
CAATCGCCAG TTTACCAGCA GACTTCTCAC ATGATGAGTG CATTGTCTAC CAATGAGGAT 
ATGCAAATGC AGTGTGAATT GTTTTCTTCT CCTCCTGCAG TTTCTGGAAA TGAAACTTCT 
ACAACTACCA CACAGCAGGT TGCAACCCCT GGCACTACCA TGTTTCAGAC ATCAAGTTCA 
GGAGATGGAG AAGAAACTGG AACACAAGCA AAACAGATTC AGAACAGTGT CTTTCAGACC 
ATGGTCCAAA TGCAACATAG TGGGGACAAT CAACCTCAAG TTAACCTTTT TTCATCCACA 
AAAAGTATGA TGAGTGTTCA GAATAGTGGT ACCCAACAAC AAGGTAATGG TTTATTCCAG 
CAAGGGAATG AGATGATGTC ACTTCAATCT GGAAATTTTT TGCAGCAGTC TTCTCATTCA 
CAGGCCCAAC TTTTTCATCC TCAAAATCCT ATTGCCGATG CTCAGAACCT TTCCCAGGAA 
ACTCAAGGTT CTCTCTTTCA TAGTCCAAAT CCTATTGTCC ACAGTCAGAC TTCTACAACC 
TCCTCTGAAC AAATGCAGCC TCCAATGTTT CACTCTCAAA GTACCATTGC TGTGTTACAG 
GGCTCTTCAG TTCCTCAAGA CCAGCAGTCA ACCAACATAT TTCTTTCCCA GAGTCCCATG 
AATAATCTTC AGACTAACAC AGTAGCCCAA GAAGCATTTT TTGCAGCACC GAACTCAATT 
TCTCCACTTC AGTCAACATC AAACAGTGAA CAACAAGCTG CTTTCCAACA GCAAGCTCCA 
ATATCACACA TCCAGACTCC TATGCTTTCC CAAGAACAGG CACAACCCCC GCAGCAGGGT 
TTATTTCAGC CTCAGGTGGC CCTGGGCTCC CTTCCACCTA ATCCAATGCC TCAAAGCCAA 
CAAGGAACCA TGTTCCAGTC ACAGCACTCA ATAGTTGCCA TGCAGAGTAA CTCTCCATCC 
CAGGAACAGC AGCAGCAGCA GCAACAGCAG CAGCAACAGC AGCAGCAACA ACAACAGAGC 
ATTTTATTCA GTAATCAGAA TACCATGGCT ACAATGGCGT CTCCAAAGCA ACCACCACCA 
AACATGATAT TCAACCCAAA TCAAAATCCA ATGGCTAATC AGGAGCAACA GAACCAGTCA 
ATTTTTCACC AACAAAGTAA CATGGCCCCA ATGAATCAAG AGCAACAGCC CATGCAATTT 
CAGAGTCAGT CCACAGTTTC CTCACTTCAG AACCCAGGTC CTACCCAGTC GGAATCATCA 
CAGACCCCCT TGTTCCATAG CTCTCCTCAG ATTCAGTTGG TACAAGGGTC ACCTAGTTCT 
CAAGAGCAGC AAGTAACTCT CTTCTTATCT CCAGCATCCA TGTCTGCCTT GCAGACCAGT 
ATAAATCAAC AAGATATGCA ACAGTCTCCT CTTTATTCCC CTCAGAACAA CATGCCTGGA 
ATTCAAGGAG CCACATCTTC GCCTCAACCA CAGGCTACTT TATTTCACAA CACAGCAGGA 
GGCACAATGA ACCAACTGCA GAATTCTCCT GGCTCATCTC AGCAGACATC AGGAATGTTC 
TTATTTGGCA TTCAAAATAA CTGTAGTCAG CTTTTAACCT CTGGACCAGC TACATTGCCT 
GATCAGTTGA TGGCCATAAG TCAGCCAGGC CAACCACAAA ACGAGGGCCA GCCACCTGTG 
ACAACACTTC TTTCTCAGCA AATGCCAGAG AATTCTCCAC TGGCATCCTC TATAAACACC 
AACCAGAACA TCGAAAAGAT TGATTTGCTT GTTTCATTGC AAAACCAAGG GAACAACTTG 
ACTGGCTCCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cleavage of osmosensitive transcriptional factor NFAT5 by Coxsackieviral protease 2A promotes viral replication.
PLoS pathogens13(12)e1006744(2017 Dec)
Qiu Y,Ye X,Zhang HM,Hanson P,Zhao G,Tong L,Xie R,Yang D

Tonicity-Responsive Enhancer-Binding Protein Mediates Hyperglycemia-Induced Inflammation and Vascular and Renal Injury.
Journal of the American Society of Nephrology : JASN(2017 Nov)
Choi SY,Lim SW,Salimi S,Yoo EJ,Lee-Kwon W,Lee HH,Lee JH,Mitchell BD,Sanada S,Parsa A,Kwon HM

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NFAT5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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