CETN1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CETN1
Entrez Gene ID	1068
Full Name	centrin 1
Synonyms	CEN1,CETN
General protein information	Preferred Names centrin 1 Names centrin-1 EF-hand protein calcium binding protein caltractin centrin, EF-hand protein, 1 testicular tissue protein Li 37
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18p11.32
Summary	The protein encoded by this gene plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. This protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle. [provided by RefSeq, Jan 2015].
Disorder MIM:	603187

mRNA and Protein(s)

mRNA	Protein	Name
NM_004066.3	NP_004057.1	centrin-1
NM_004066.2	NP_004057.1	centrin-1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CETN1	XP_547653.1
Pan troglodytes (chimpanzee)	CETN1	XP_523881.1
	CETN1	XP_001089502.1
Arabidopsis thaliana (thale cress)	CEN2	NP_001031798.1
Bos taurus (cattle)	CETN1	NP_001072974.1
Mus musculus (house mouse)	Cetn1	NP_031619.3
Danio rerio (zebrafish)	cetn2	XP_002667227.2
Homo sapiens (human)	CETN1	NP_004057.1
Rattus norvegicus (Norway rat)	Cetn1	NP_445913.1
Gallus gallus (chicken)	CETN1	NP_001264849.1
Oryza sativa (rice)	Os10g0389000	NP_001064498.2

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Optimization of purification method and characterization of recombinant human Centrin-1.
Phanindranath R, Sudhakar DV, Sharma AK, Thangaraj K, Sharma Y
Protein expression and purification12448-54(2016 Aug)

A chirality change in XPC- and Sfi1-derived peptides affects their affinity for centrin.
Grecu D, Irudayaraj VP, Martinez-Sanz J, Mallet JM, Assairi L
Peptides7877-86(2016 Apr)

Knockdown of CETN1 inhibits breast cancer cells proliferation.
Shuangta X, Yali H, Zhengrong Z, Qingquan Z, Xiaoshan Z, Fanghui Y, Ng W, Yinghui L, Jianhua X
Journal of B.U.ON. : official journal of the Balkan Union of Oncology19(3)656-61(2014 Jul-Sep)

The CP110-interacting proteins Talpid3 and Cep290 play overlapping and distinct roles in cilia assembly.
Kobayashi T, Kim S, Lin YC, Inoue T, Dynlacht BD
The Journal of cell biology204(2)215-29(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

important spectral and hydrodynamic characteristics of human Centrin-1, accounting that HsCentrin-1 has moderate affinity for Ca(2+). Centrin-1 does not gain structure as seen by far- and near-UV circular dichroism, rather there is a loss of ellipticity, though inconsiderable upon binding Ca(2+).
Title: Optimization of purification method and characterization of recombinant human Centrin-1.

A chirality change in XPC- and Sfi1-derived peptides affects their affinity for centrin.
Title: A chirality change in XPC- and Sfi1-derived peptides affects their affinity for centrin.

CETN1 is overexpressed in breast cancer tissue and promotes cells'proliferation, tumor growth and metastasis.
Title: Knockdown of CETN1 inhibits breast cancer cells proliferation.

These data demonstrate a functional role for CaM binding to CP110 and suggest that CP110 cooperates with CaM and centrin to regulate progression through cytokinesis.
Title: CP110 cooperates with two calcium-binding proteins to regulate cytokinesis and genome stability.

The following CETN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CETN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16276	NM_004066.3 Latest version!	Homo sapiens centrin 1 (CETN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu16276	NM_004066.2	Homo sapiens centrin 1 (CETN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CETN1 ( NM_004066.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004057.1 Homo sapiens centrin 1 (CETN1), mRNA.

Price & Availability↑

CloneID	OHu16276
Clone ID Related Accession (Same CDS sequence)	NM_004066.3 , NM_004066.2
Accession Version	NM_004066.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	centrin-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC029515.1, AP001178.4 and AI198628.1. On May 17, 2019 this sequence version replaced NM_004066.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: SRR5189667.349026.1, SRR5189667.282530.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000327228.5/ ENSP00000319052.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCCG GCTTCAAGAA GCCCAGCGCT GCCTCCACCG GCCAAAAGAG AAAGGTGGCA 
CCTAAGCCCG AGCTCACTGA GGATCAGAAG CAAGAAGTTC GGGAAGCATT TGACCTCTTC 
GACGTGGACG GAAGTGGGAC CATCGACGCG AAGGAGCTGA AGGTGGCCAT GAGAGCGCTG 
GGCTTCGAAC CCAGGAAGGA AGAGATGAAG AAAATGATCT CCGAGGTGGA CAGGGAAGGC 
ACGGGGAAGA TCAGCTTCAA TGACTTCCTG GCCGTGATGA CGCAGAAGAT GTCCGAGAAG 
GACACCAAAG AAGAAATCCT GAAGGCCTTC AGGCTCTTTG ATGACGATGA GACCGGGAAG 
ATCTCGTTCA AAAACCTGAA GCGTGTGGCC AACGAGCTGG GGGAGAACCT CACGGATGAG 
GAGCTGCAGG AGATGATCGA CGAAGCTGAT CGGGATGGGG ACGGCGAAGT GAACGAGGAG 
GAGTTCCTTC GGATCATGAA GAAGACCAGC CTTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004057.1
CDS	30..548
Translation	MASGFKKPSAASTGQKRKVAPKPELTEDQKQEVREAFDLFDVDGSGTIDAKELKVAMRALGFEPRKEEMKKMISEVDREGTGKISFNDFLAVMTQKMSEKDTKEEILKAFRLFDDDETGKISFKNLKRVANELGENLTDEELQEMIDEADRDGDGEVNEEEFLRIMKKTSLY

Target ORF information:

RefSeq Version	NM_004066.3
Organism	Homo sapiens(human)
Definition	Homo sapiens centrin 1 (CETN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004066.3

ORF Insert Sequence:

ATGGCTTCCG GCTTCAAGAA GCCCAGCGCT GCCTCCACCG GCCAAAAGAG AAAGGTGGCA 
CCTAAGCCCG AGCTCACTGA GGATCAGAAG CAAGAAGTTC GGGAAGCATT TGACCTCTTC 
GACGTGGACG GAAGTGGGAC CATCGACGCG AAGGAGCTGA AGGTGGCCAT GAGAGCGCTG 
GGCTTCGAAC CCAGGAAGGA AGAGATGAAG AAAATGATCT CCGAGGTGGA CAGGGAAGGC 
ACGGGGAAGA TCAGCTTCAA TGACTTCCTG GCCGTGATGA CGCAGAAGAT GTCCGAGAAG 
GACACCAAAG AAGAAATCCT GAAGGCCTTC AGGCTCTTTG ATGACGATGA GACCGGGAAG 
ATCTCGTTCA AAAACCTGAA GCGTGTGGCC AACGAGCTGG GGGAGAACCT CACGGATGAG 
GAGCTGCAGG AGATGATCGA CGAAGCTGAT CGGGATGGGG ACGGCGAAGT GAACGAGGAG 
GAGTTCCTTC GGATCATGAA GAAGACCAGC CTTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CETN1 ( NM_004066.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004057.1 Homo sapiens centrin 1 (CETN1), mRNA.

Price & Availability↑

CloneID	OHu16276
Clone ID Related Accession (Same CDS sequence)	NM_004066.3 , NM_004066.2
Accession Version	NM_004066.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	centrin-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB448454.1, BC029515.1, AP001178.4 and AI198628.1. On Jan 7, 2015 this sequence version replaced NM_004066.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGGCTTCCG GCTTCAAGAA GCCCAGCGCT GCCTCCACCG GCCAAAAGAG AAAGGTGGCA 
CCTAAGCCCG AGCTCACTGA GGATCAGAAG CAAGAAGTTC GGGAAGCATT TGACCTCTTC 
GACGTGGACG GAAGTGGGAC CATCGACGCG AAGGAGCTGA AGGTGGCCAT GAGAGCGCTG 
GGCTTCGAAC CCAGGAAGGA AGAGATGAAG AAAATGATCT CCGAGGTGGA CAGGGAAGGC 
ACGGGGAAGA TCAGCTTCAA TGACTTCCTG GCCGTGATGA CGCAGAAGAT GTCCGAGAAG 
GACACCAAAG AAGAAATCCT GAAGGCCTTC AGGCTCTTTG ATGACGATGA GACCGGGAAG 
ATCTCGTTCA AAAACCTGAA GCGTGTGGCC AACGAGCTGG GGGAGAACCT CACGGATGAG 
GAGCTGCAGG AGATGATCGA CGAAGCTGAT CGGGATGGGG ACGGCGAAGT GAACGAGGAG 
GAGTTCCTTC GGATCATGAA GAAGACCAGC CTTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004057.1
CDS	67..585
Translation	MASGFKKPSAASTGQKRKVAPKPELTEDQKQEVREAFDLFDVDGSGTIDAKELKVAMRALGFEPRKEEMKKMISEVDREGTGKISFNDFLAVMTQKMSEKDTKEEILKAFRLFDDDETGKISFKNLKRVANELGENLTDEELQEMIDEADRDGDGEVNEEEFLRIMKKTSLY

Target ORF information:

RefSeq Version	NM_004066.2
Organism	Homo sapiens(human)
Definition	Homo sapiens centrin 1 (CETN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004066.2

ORF Insert Sequence:

ATGGCTTCCG GCTTCAAGAA GCCCAGCGCT GCCTCCACCG GCCAAAAGAG AAAGGTGGCA 
CCTAAGCCCG AGCTCACTGA GGATCAGAAG CAAGAAGTTC GGGAAGCATT TGACCTCTTC 
GACGTGGACG GAAGTGGGAC CATCGACGCG AAGGAGCTGA AGGTGGCCAT GAGAGCGCTG 
GGCTTCGAAC CCAGGAAGGA AGAGATGAAG AAAATGATCT CCGAGGTGGA CAGGGAAGGC 
ACGGGGAAGA TCAGCTTCAA TGACTTCCTG GCCGTGATGA CGCAGAAGAT GTCCGAGAAG 
GACACCAAAG AAGAAATCCT GAAGGCCTTC AGGCTCTTTG ATGACGATGA GACCGGGAAG 
ATCTCGTTCA AAAACCTGAA GCGTGTGGCC AACGAGCTGG GGGAGAACCT CACGGATGAG 
GAGCTGCAGG AGATGATCGA CGAAGCTGAT CGGGATGGGG ACGGCGAAGT GAACGAGGAG 
GAGTTCCTTC GGATCATGAA GAAGACCAGC CTTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Optimization of purification method and characterization of recombinant human Centrin-1.
Protein expression and purification12448-54(2016 Aug)
Phanindranath R,Sudhakar DV,Sharma AK,Thangaraj K,Sharma Y

A chirality change in XPC- and Sfi1-derived peptides affects their affinity for centrin.
Peptides7877-86(2016 Apr)
Grecu D,Irudayaraj VP,Martinez-Sanz J,Mallet JM,Assairi L

Knockdown of CETN1 inhibits breast cancer cells proliferation.
Journal of B.U.ON. : official journal of the Balkan Union of Oncology19(3)656-61(2014 Jul-Sep)
Shuangta X,Yali H,Zhengrong Z,Qingquan Z,Xiaoshan Z,Fanghui Y,Ng W,Yinghui L,Jianhua X

The CP110-interacting proteins Talpid3 and Cep290 play overlapping and distinct roles in cilia assembly.
The Journal of cell biology204(2)215-29(2014 Jan)
Kobayashi T,Kim S,Lin YC,Inoue T,Dynlacht BD

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CETN1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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