CCT4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CCT4
Entrez Gene ID	10575
Full Name	chaperonin containing TCP1 subunit 4
Synonyms	CCT-DELTA,Cctd,SRB
General protein information	Preferred Names chaperonin containing TCP1 subunit 4 Names T-complex protein 1 subunit delta TCP-1-delta chaperonin containing TCP1, subunit 4 (delta) chaperonin containing t-complex polypeptide 1, delta subunit stimulator of TAR RNA-binding
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p15
Summary	The chaperonin containing TCP1 (MIM 186980) complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin (see MIM 191130), as well as alpha-transducin (MIM 139330) (Won et al., 1998 [PubMed 9819444]).[supplied by OMIM, Mar 2008].
Disorder MIM:	605142

mRNA and Protein(s)

mRNA	Protein	Name
NM_006430.4	NP_006421.2	T-complex protein 1 subunit delta isoform a
NM_006430.3	NP_006421.2	T-complex protein 1 subunit delta isoform a
NM_001256721.1	NP_001243650.1	T-complex protein 1 subunit delta isoform b

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CCT4	XP_003639378.1
Bos taurus (cattle)	CCT4	NP_001033283.1
Gallus gallus (chicken)	CCT4	NP_996761.1
Eremothecium gossypii	AGOS_ADR083W	NP_984179.1
Schizosaccharomyces pombe (fission yeast)	cct4	NP_595155.1
Drosophila melanogaster (fruit fly)	CG5525	NP_609579.1
Caenorhabditis elegans (roundworm)	cct-4	NP_495750.1
Saccharomyces cerevisiae (baker's yeast)	CCT4	NP_010138.1
Xenopus tropicalis (tropical clawed frog)	cct4	NP_001006852.1
	CCT4	XP_001114200.2
Rattus norvegicus (Norway rat)	Cct4	NP_877966.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009505	XP_310191.2
Arabidopsis thaliana (thale cress)	AT3G18190	NP_188447.1
Oryza sativa (rice)	Os02g0332200	NP_001046725.1
Homo sapiens (human)	CCT4	NP_006421.2
Pan troglodytes (chimpanzee)	CCT4	XP_001152144.3
Mus musculus (house mouse)	Cct4	NP_033967.1
Danio rerio (zebrafish)	cct4	NP_956877.1
Kluyveromyces lactis	KLLA0F05005g	XP_455307.1
Magnaporthe oryzae (rice blast fungus)	MGG_01582	XP_003714560.1
Neurospora crassa	NCU02839	XP_964129.1
Oryza sativa (rice)	Os10g0514600	NP_001065054.1

Gene Ontology
Bibliography

Related articles in PubMed

Proteostatic control of telomerase function through TRiC-mediated folding of TCAB1.
Freund A, Zhong FL, Venteicher AS, Meng Z, Veenstra TD, Frydman J, Artandi SE
Cell159(6)1389-403(2014 Dec)

Chaperonin-containing TCP-1 complex directly binds to the cytoplasmic domain of the LOX-1 receptor.
Bakthavatsalam D, Soung RH, Tweardy DJ, Chiu W, Dixon RA, Woodside DG
FEBS letters588(13)2133-40(2014 Jun)

The chaperonin CCT interacts with and mediates the correct folding and activity of three subunits of translation initiation factor eIF3: b, i and h.
Roobol A, Roobol J, Carden MJ, Smith ME, Hershey JW, Bastide A, Knight JR, Willis AE, Smales CM
The Biochemical journal458(2)213-24(2014 Mar)

Human CCT4 and CCT5 chaperonin subunits expressed in Escherichia coli form biologically active homo-oligomers.
Sergeeva OA, Chen B, Haase-Pettingell C, Ludtke SJ, Chiu W, King JA
The Journal of biological chemistry288(24)17734-44(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Chaperonin CCT is required for the correct folding of eIF3h and eIF3i subunits.
Title: The chaperonin CCT interacts with and mediates the correct folding and activity of three subunits of translation initiation factor eIF3: b, i and h.

Both CCT4 and CCT5 homo-oligomers have the property of forming 8-fold double rings absent the other subunits, and these complexes carry out chaperonin reactions without other partner subunits.
Title: Human CCT4 and CCT5 chaperonin subunits expressed in Escherichia coli form biologically active homo-oligomers.

The following CCT4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCT4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15890	NM_006430.4 Latest version!	Homo sapiens chaperonin containing TCP1 subunit 4 (CCT4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu15797	NM_001256721.1 Latest version!	Homo sapiens chaperonin containing TCP1 subunit 4 (CCT4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu15890	NM_006430.3	Homo sapiens chaperonin containing TCP1 subunit 4 (CCT4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CCT4 ( NM_006430.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006421.2 Homo sapiens chaperonin containing TCP1 subunit 4 (CCT4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15890
Clone ID Related Accession (Same CDS sequence)	NM_006430.4 , NM_006430.3
Accession Version	NM_006430.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	T-complex protein 1 subunit delta isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB461009.1, BC106934.1, AI439570.1 and AC107081.5. On Nov 22, 2018 this sequence version replaced NM_006430.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.125403.1, SRR1803617.1740.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000394440.8/ ENSP00000377958.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCGAGA ATGTGGCACC CCGGAGCGGG GCGACTGCCG GGGCTGCCGG CGGCCGCGGG 
AAAGGCGCCT ATCAGGACCG CGACAAGCCA GCCCAGATCC GCTTCAGCAA CATTTCCGCC 
GCCAAAGCGG TTGCTGATGC TATTAGAACA AGCCTTGGAC CAAAAGGAAT GGATAAAATG 
ATTCAAGATG GAAAAGGTGA TGTAACCATT ACAAATGATG GTGCTACCAT TCTGAAACAA 
ATGCAAGTAT TACATCCAGC AGCCAGAATG CTGGTGGAGC TGTCTAAGGC TCAAGATATA 
GAAGCAGGAG ATGGCACCAC ATCAGTAGTC ATCATTGCTG GCTCCCTCTT AGATTCTTGT 
ACCAAGCTTC TTCAGAAAGG GATTCATCCA ACCATCATTT CTGAGTCATT CCAGAAGGCC 
CTGGAAAAGG GCATTGAAAT CTTGACTGAC ATGTCTCGAC CTGTGGAACT GAGTGACAGA 
GAAACTTTGT TAAATAGTGC AACCACTTCA CTGAACTCAA AGGTGGTTTC TCAGTATTCA 
AGTCTGCTTT CTCCAATGAG TGTAAATGCA GTGATGAAAG TGATTGACCC AGCCACAGCC 
ACCAGTGTAG ATCTTAGAGA TATTAAAATA GTTAAGAAGC TTGGTGGGAC AATTGATGAC 
TGTGAGTTGG TGGAAGGGCT GGTTCTCACC CAAAAAGTGT CAAATTCTGG CATAACCAGA 
GTTGAAAAGG CCAAGATTGG GCTTATTCAG TTTTGCTTAT CTGCTCCCAA AACAGACATG 
GATAATCAAA TAGTGGTTTC TGACTATGCC CAGATGGACC GAGTGCTGCG AGAAGAGAGA 
GCCTATATTT TAAATTTAGT GAAGCAAATT AAAAAAACAG GATGTAATGT CCTTCTCATA 
CAGAAATCTA TTCTAAGAGA TGCTCTTAGT GATCTTGCAT TACACTTTCT GAATAAAATG 
AAGATCATGG TGATTAAGGA TATTGAAAGA GAAGACATTG AATTCATTTG TAAGACAATT 
GGAACCAAGC CAGTTGCTCA TATTGACCAA TTTACTGCTG ACATGCTGGG TTCTGCTGAG 
TTAGCTGAGG AGGTCAATTT AAATGGTTCT GGCAAACTGC TCAAGATTAC AGGCTGTGCC 
AGCCCTGGAA AAACAGTTAC AATTGTTGTT CGTGGTTCTA ACAAACTGGT GATTGAAGAA 
GCTGAGCGCT CCATTCATGA TGCCCTATGT GTTATTCGTT GTTTAGTGAA GAAGAGGGCT 
CTTATTGCAG GAGGTGGTGC TCCAGAAATA GAGTTGGCCC TACGATTAAC TGAATATTCA 
CGAACACTGA GTGGTATGGA ATCCTACTGC GTTCGTGCTT TTGCAGATGC TATGGAGGTC 
ATTCCATCTA CACTAGCTGA AAATGCCGGC CTGAATCCCA TTTCTACAGT AACAGAACTA 
AGAAACCGGC ATGCCCAGGG AGAAAAAACT GCAGGCATTA ATGTCCGAAA GGGTGGTATT 
TCCAACATTT TGGAGGAACT GGTTGTCCAG CCTCTGTTGG TATCAGTCAG TGCTCTGACT 
CTTGCAACTG AAACTGTTCG GAGCATTCTG AAAATAGATG ATGTGGTAAA CACTCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006421.2
CDS	150..1769
Translation	MPENVAPRSGATAGAAGGRGKGAYQDRDKPAQIRFSNISAAKAVADAIRTSLGPKGMDKMIQDGKGDVTITNDGATILKQMQVLHPAARMLVELSKAQDIEAGDGTTSVVIIAGSLLDSCTKLLQKGIHPTIISESFQKALEKGIEILTDMSRPVELSDRETLLNSATTSLNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVSNSGITRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALSDLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTADMLGSAELAEEVNLNGSGKLLKITGCASPGKTVTIVVRGSNKLVIEEAERSIHDALCVIRCLVKKRALIAGGGAPEIELALRLTEYSRTLSGMESYCVRAFADAMEVIPSTLAENAGLNPISTVTELRNRHAQGEKTAGINVRKGGISNILEELVVQPLLVSVSALTLATETVRSILKIDDVVNTR

Target ORF information:

RefSeq Version	NM_006430.4
Organism	Homo sapiens(human)
Definition	Homo sapiens chaperonin containing TCP1 subunit 4 (CCT4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006430.4

ORF Insert Sequence:

ATGCCCGAGA ATGTGGCACC CCGGAGCGGG GCGACTGCCG GGGCTGCCGG CGGCCGCGGG 
AAAGGCGCCT ATCAGGACCG CGACAAGCCA GCCCAGATCC GCTTCAGCAA CATTTCCGCC 
GCCAAAGCGG TTGCTGATGC TATTAGAACA AGCCTTGGAC CAAAAGGAAT GGATAAAATG 
ATTCAAGATG GAAAAGGTGA TGTAACCATT ACAAATGATG GTGCTACCAT TCTGAAACAA 
ATGCAAGTAT TACATCCAGC AGCCAGAATG CTGGTGGAGC TGTCTAAGGC TCAAGATATA 
GAAGCAGGAG ATGGCACCAC ATCAGTAGTC ATCATTGCTG GCTCCCTCTT AGATTCTTGT 
ACCAAGCTTC TTCAGAAAGG GATTCATCCA ACCATCATTT CTGAGTCATT CCAGAAGGCC 
CTGGAAAAGG GCATTGAAAT CTTGACTGAC ATGTCTCGAC CTGTGGAACT GAGTGACAGA 
GAAACTTTGT TAAATAGTGC AACCACTTCA CTGAACTCAA AGGTGGTTTC TCAGTATTCA 
AGTCTGCTTT CTCCAATGAG TGTAAATGCA GTGATGAAAG TGATTGACCC AGCCACAGCC 
ACCAGTGTAG ATCTTAGAGA TATTAAAATA GTTAAGAAGC TTGGTGGGAC AATTGATGAC 
TGTGAGTTGG TGGAAGGGCT GGTTCTCACC CAAAAAGTGT CAAATTCTGG CATAACCAGA 
GTTGAAAAGG CCAAGATTGG GCTTATTCAG TTTTGCTTAT CTGCTCCCAA AACAGACATG 
GATAATCAAA TAGTGGTTTC TGACTATGCC CAGATGGACC GAGTGCTGCG AGAAGAGAGA 
GCCTATATTT TAAATTTAGT GAAGCAAATT AAAAAAACAG GATGTAATGT CCTTCTCATA 
CAGAAATCTA TTCTAAGAGA TGCTCTTAGT GATCTTGCAT TACACTTTCT GAATAAAATG 
AAGATCATGG TGATTAAGGA TATTGAAAGA GAAGACATTG AATTCATTTG TAAGACAATT 
GGAACCAAGC CAGTTGCTCA TATTGACCAA TTTACTGCTG ACATGCTGGG TTCTGCTGAG 
TTAGCTGAGG AGGTCAATTT AAATGGTTCT GGCAAACTGC TCAAGATTAC AGGCTGTGCC 
AGCCCTGGAA AAACAGTTAC AATTGTTGTT CGTGGTTCTA ACAAACTGGT GATTGAAGAA 
GCTGAGCGCT CCATTCATGA TGCCCTATGT GTTATTCGTT GTTTAGTGAA GAAGAGGGCT 
CTTATTGCAG GAGGTGGTGC TCCAGAAATA GAGTTGGCCC TACGATTAAC TGAATATTCA 
CGAACACTGA GTGGTATGGA ATCCTACTGC GTTCGTGCTT TTGCAGATGC TATGGAGGTC 
ATTCCATCTA CACTAGCTGA AAATGCCGGC CTGAATCCCA TTTCTACAGT AACAGAACTA 
AGAAACCGGC ATGCCCAGGG AGAAAAAACT GCAGGCATTA ATGTCCGAAA GGGTGGTATT 
TCCAACATTT TGGAGGAACT GGTTGTCCAG CCTCTGTTGG TATCAGTCAG TGCTCTGACT 
CTTGCAACTG AAACTGTTCG GAGCATTCTG AAAATAGATG ATGTGGTAAA CACTCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCT4 ( NM_006430.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006421.2 Homo sapiens chaperonin containing TCP1 subunit 4 (CCT4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15890
Clone ID Related Accession (Same CDS sequence)	NM_006430.4 , NM_006430.3
Accession Version	NM_006430.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	T-complex protein 1 subunit delta isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB461009.1, BC106934.1 and AI439570.1. On Feb 15, 2012 this sequence version replaced NM_006430.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.218473.1, SRR1803615.41908.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCCCGAGA ATGTGGCACC CCGGAGCGGG GCGACTGCCG GGGCTGCCGG CGGCCGCGGG 
AAAGGCGCCT ATCAGGACCG CGACAAGCCA GCCCAGATCC GCTTCAGCAA CATTTCCGCC 
GCCAAAGCGG TTGCTGATGC TATTAGAACA AGCCTTGGAC CAAAAGGAAT GGATAAAATG 
ATTCAAGATG GAAAAGGTGA TGTAACCATT ACAAATGATG GTGCTACCAT TCTGAAACAA 
ATGCAAGTAT TACATCCAGC AGCCAGAATG CTGGTGGAGC TGTCTAAGGC TCAAGATATA 
GAAGCAGGAG ATGGCACCAC ATCAGTAGTC ATCATTGCTG GCTCCCTCTT AGATTCTTGT 
ACCAAGCTTC TTCAGAAAGG GATTCATCCA ACCATCATTT CTGAGTCATT CCAGAAGGCC 
CTGGAAAAGG GCATTGAAAT CTTGACTGAC ATGTCTCGAC CTGTGGAACT GAGTGACAGA 
GAAACTTTGT TAAATAGTGC AACCACTTCA CTGAACTCAA AGGTGGTTTC TCAGTATTCA 
AGTCTGCTTT CTCCAATGAG TGTAAATGCA GTGATGAAAG TGATTGACCC AGCCACAGCC 
ACCAGTGTAG ATCTTAGAGA TATTAAAATA GTTAAGAAGC TTGGTGGGAC AATTGATGAC 
TGTGAGTTGG TGGAAGGGCT GGTTCTCACC CAAAAAGTGT CAAATTCTGG CATAACCAGA 
GTTGAAAAGG CCAAGATTGG GCTTATTCAG TTTTGCTTAT CTGCTCCCAA AACAGACATG 
GATAATCAAA TAGTGGTTTC TGACTATGCC CAGATGGACC GAGTGCTGCG AGAAGAGAGA 
GCCTATATTT TAAATTTAGT GAAGCAAATT AAAAAAACAG GATGTAATGT CCTTCTCATA 
CAGAAATCTA TTCTAAGAGA TGCTCTTAGT GATCTTGCAT TACACTTTCT GAATAAAATG 
AAGATCATGG TGATTAAGGA TATTGAAAGA GAAGACATTG AATTCATTTG TAAGACAATT 
GGAACCAAGC CAGTTGCTCA TATTGACCAA TTTACTGCTG ACATGCTGGG TTCTGCTGAG 
TTAGCTGAGG AGGTCAATTT AAATGGTTCT GGCAAACTGC TCAAGATTAC AGGCTGTGCC 
AGCCCTGGAA AAACAGTTAC AATTGTTGTT CGTGGTTCTA ACAAACTGGT GATTGAAGAA 
GCTGAGCGCT CCATTCATGA TGCCCTATGT GTTATTCGTT GTTTAGTGAA GAAGAGGGCT 
CTTATTGCAG GAGGTGGTGC TCCAGAAATA GAGTTGGCCC TACGATTAAC TGAATATTCA 
CGAACACTGA GTGGTATGGA ATCCTACTGC GTTCGTGCTT TTGCAGATGC TATGGAGGTC 
ATTCCATCTA CACTAGCTGA AAATGCCGGC CTGAATCCCA TTTCTACAGT AACAGAACTA 
AGAAACCGGC ATGCCCAGGG AGAAAAAACT GCAGGCATTA ATGTCCGAAA GGGTGGTATT 
TCCAACATTT TGGAGGAACT GGTTGTCCAG CCTCTGTTGG TATCAGTCAG TGCTCTGACT 
CTTGCAACTG AAACTGTTCG GAGCATTCTG AAAATAGATG ATGTGGTAAA CACTCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006421.2
CDS	165..1784
Translation	MPENVAPRSGATAGAAGGRGKGAYQDRDKPAQIRFSNISAAKAVADAIRTSLGPKGMDKMIQDGKGDVTITNDGATILKQMQVLHPAARMLVELSKAQDIEAGDGTTSVVIIAGSLLDSCTKLLQKGIHPTIISESFQKALEKGIEILTDMSRPVELSDRETLLNSATTSLNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVSNSGITRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALSDLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTADMLGSAELAEEVNLNGSGKLLKITGCASPGKTVTIVVRGSNKLVIEEAERSIHDALCVIRCLVKKRALIAGGGAPEIELALRLTEYSRTLSGMESYCVRAFADAMEVIPSTLAENAGLNPISTVTELRNRHAQGEKTAGINVRKGGISNILEELVVQPLLVSVSALTLATETVRSILKIDDVVNTR

Target ORF information:

RefSeq Version	NM_006430.3
Organism	Homo sapiens(human)
Definition	Homo sapiens chaperonin containing TCP1 subunit 4 (CCT4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006430.3

ORF Insert Sequence:

ATGCCCGAGA ATGTGGCACC CCGGAGCGGG GCGACTGCCG GGGCTGCCGG CGGCCGCGGG 
AAAGGCGCCT ATCAGGACCG CGACAAGCCA GCCCAGATCC GCTTCAGCAA CATTTCCGCC 
GCCAAAGCGG TTGCTGATGC TATTAGAACA AGCCTTGGAC CAAAAGGAAT GGATAAAATG 
ATTCAAGATG GAAAAGGTGA TGTAACCATT ACAAATGATG GTGCTACCAT TCTGAAACAA 
ATGCAAGTAT TACATCCAGC AGCCAGAATG CTGGTGGAGC TGTCTAAGGC TCAAGATATA 
GAAGCAGGAG ATGGCACCAC ATCAGTAGTC ATCATTGCTG GCTCCCTCTT AGATTCTTGT 
ACCAAGCTTC TTCAGAAAGG GATTCATCCA ACCATCATTT CTGAGTCATT CCAGAAGGCC 
CTGGAAAAGG GCATTGAAAT CTTGACTGAC ATGTCTCGAC CTGTGGAACT GAGTGACAGA 
GAAACTTTGT TAAATAGTGC AACCACTTCA CTGAACTCAA AGGTGGTTTC TCAGTATTCA 
AGTCTGCTTT CTCCAATGAG TGTAAATGCA GTGATGAAAG TGATTGACCC AGCCACAGCC 
ACCAGTGTAG ATCTTAGAGA TATTAAAATA GTTAAGAAGC TTGGTGGGAC AATTGATGAC 
TGTGAGTTGG TGGAAGGGCT GGTTCTCACC CAAAAAGTGT CAAATTCTGG CATAACCAGA 
GTTGAAAAGG CCAAGATTGG GCTTATTCAG TTTTGCTTAT CTGCTCCCAA AACAGACATG 
GATAATCAAA TAGTGGTTTC TGACTATGCC CAGATGGACC GAGTGCTGCG AGAAGAGAGA 
GCCTATATTT TAAATTTAGT GAAGCAAATT AAAAAAACAG GATGTAATGT CCTTCTCATA 
CAGAAATCTA TTCTAAGAGA TGCTCTTAGT GATCTTGCAT TACACTTTCT GAATAAAATG 
AAGATCATGG TGATTAAGGA TATTGAAAGA GAAGACATTG AATTCATTTG TAAGACAATT 
GGAACCAAGC CAGTTGCTCA TATTGACCAA TTTACTGCTG ACATGCTGGG TTCTGCTGAG 
TTAGCTGAGG AGGTCAATTT AAATGGTTCT GGCAAACTGC TCAAGATTAC AGGCTGTGCC 
AGCCCTGGAA AAACAGTTAC AATTGTTGTT CGTGGTTCTA ACAAACTGGT GATTGAAGAA 
GCTGAGCGCT CCATTCATGA TGCCCTATGT GTTATTCGTT GTTTAGTGAA GAAGAGGGCT 
CTTATTGCAG GAGGTGGTGC TCCAGAAATA GAGTTGGCCC TACGATTAAC TGAATATTCA 
CGAACACTGA GTGGTATGGA ATCCTACTGC GTTCGTGCTT TTGCAGATGC TATGGAGGTC 
ATTCCATCTA CACTAGCTGA AAATGCCGGC CTGAATCCCA TTTCTACAGT AACAGAACTA 
AGAAACCGGC ATGCCCAGGG AGAAAAAACT GCAGGCATTA ATGTCCGAAA GGGTGGTATT 
TCCAACATTT TGGAGGAACT GGTTGTCCAG CCTCTGTTGG TATCAGTCAG TGCTCTGACT 
CTTGCAACTG AAACTGTTCG GAGCATTCTG AAAATAGATG ATGTGGTAAA CACTCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCT4 ( NM_001256721.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243650.1 Homo sapiens chaperonin containing TCP1 subunit 4 (CCT4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15797
Clone ID Related Accession (Same CDS sequence)	NM_001256721.1
Accession Version	NM_001256721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Homo sapiens(human)
Product	T-complex protein 1 subunit delta isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB461009.1, AK303082.1, BM765256.1, AA136129.1 and AI439570.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant. It encodes isoform b which is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303082.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCCCGAGA ATGTGGCACC CCGGAGCGGG GCGACTGCCG GGGCTGCCGG CGGCCGCGGG 
AAAGGCGCCT ATCAGGACCG CGACAAGCCA GCCCAGATCC GCTTCAGCAA CATTTCCGCC 
GCCAAAGCGG TTGCTGATGC TATTAGAACA AGCCTTGGAC CAAAAGGAAT GGATAAAATG 
CTGGTGGAGC TGTCTAAGGC TCAAGATATA GAAGCAGGAG ATGGCACCAC ATCAGTAGTC 
ATCATTGCTG GCTCCCTCTT AGATTCTTGT ACCAAGCTTC TTCAGAAAGG GATTCATCCA 
ACCATCATTT CTGAGTCATT CCAGAAGGCC CTGGAAAAGG GCATTGAAAT CTTGACTGAC 
ATGTCTCGAC CTGTGGAACT GAGTGACAGA GAAACTTTGT TAAATAGTGC AACCACTTCA 
CTGAACTCAA AGGTGGTTTC TCAGTATTCA AGTCTGCTTT CTCCAATGAG TGTAAATGCA 
GTGATGAAAG TGATTGACCC AGCCACAGCC ACCAGTGTAG ATCTTAGAGA TATTAAAATA 
GTTAAGAAGC TTGGTGGGAC AATTGATGAC TGTGAGTTGG TGGAAGGGCT GGTTCTCACC 
CAAAAAGTGT CAAATTCTGG CATAACCAGA GTTGAAAAGG CCAAGATTGG GCTTATTCAG 
TTTTGCTTAT CTGCTCCCAA AACAGACATG GATAATCAAA TAGTGGTTTC TGACTATGCC 
CAGATGGACC GAGTGCTGCG AGAAGAGAGA GCCTATATTT TAAATTTAGT GAAGCAAATT 
AAAAAAACAG GATGTAATGT CCTTCTCATA CAGAAATCTA TTCTAAGAGA TGCTCTTAGT 
GATCTTGCAT TACACTTTCT GAATAAAATG AAGATCATGG TGATTAAGGA TATTGAAAGA 
GAAGACATTG AATTCATTTG TAAGACAATT GGAACCAAGC CAGTTGCTCA TATTGACCAA 
TTTACTGCTG ACATGCTGGG TTCTGCTGAG TTAGCTGAGG AGGTCAATTT AAATGGTTCT 
GGCAAACTGC TCAAGATTAC AGGCTGTGCC AGCCCTGGAA AAACAGTTAC AATTGTTGTT 
CGTGGTTCTA ACAAACTGGT GATTGAAGAA GCTGAGCGCT CCATTCATGA TGCCCTATGT 
GTTATTCGTT GTTTAGTGAA GAAGAGGGCT CTTATTGCAG GAGGTGGTGC TCCAGAAATA 
GAGTTGGCCC TACGATTAAC TGAATATTCA CGAACACTGA GTGGTATGGA ATCCTACTGC 
GTTCGTGCTT TTGCAGATGC TATGGAGGTC ATTCCATCTA CACTAGCTGA AAATGCCGGC 
CTGAATCCCA TTTCTACAGT AACAGAACTA AGAAACCGGC ATGCCCAGGG AGAAAAAACT 
GCAGGCATTA ATGTCCGAAA GGGTGGTATT TCCAACATTT TGGAGGAACT GGTTGTCCAG 
CCTCTGTTGG TATCAGTCAG TGCTCTGACT CTTGCAACTG AAACTGTTCG GAGCATTCTG 
AAAATAGATG ATGTGGTAAA CACTCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243650.1
CDS	165..1694
Translation	MPENVAPRSGATAGAAGGRGKGAYQDRDKPAQIRFSNISAAKAVADAIRTSLGPKGMDKMLVELSKAQDIEAGDGTTSVVIIAGSLLDSCTKLLQKGIHPTIISESFQKALEKGIEILTDMSRPVELSDRETLLNSATTSLNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVSNSGITRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALSDLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTADMLGSAELAEEVNLNGSGKLLKITGCASPGKTVTIVVRGSNKLVIEEAERSIHDALCVIRCLVKKRALIAGGGAPEIELALRLTEYSRTLSGMESYCVRAFADAMEVIPSTLAENAGLNPISTVTELRNRHAQGEKTAGINVRKGGISNILEELVVQPLLVSVSALTLATETVRSILKIDDVVNTR

Target ORF information:

RefSeq Version	NM_001256721.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chaperonin containing TCP1 subunit 4 (CCT4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256721.1

ORF Insert Sequence:

ATGCCCGAGA ATGTGGCACC CCGGAGCGGG GCGACTGCCG GGGCTGCCGG CGGCCGCGGG 
AAAGGCGCCT ATCAGGACCG CGACAAGCCA GCCCAGATCC GCTTCAGCAA CATTTCCGCC 
GCCAAAGCGG TTGCTGATGC TATTAGAACA AGCCTTGGAC CAAAAGGAAT GGATAAAATG 
CTGGTGGAGC TGTCTAAGGC TCAAGATATA GAAGCAGGAG ATGGCACCAC ATCAGTAGTC 
ATCATTGCTG GCTCCCTCTT AGATTCTTGT ACCAAGCTTC TTCAGAAAGG GATTCATCCA 
ACCATCATTT CTGAGTCATT CCAGAAGGCC CTGGAAAAGG GCATTGAAAT CTTGACTGAC 
ATGTCTCGAC CTGTGGAACT GAGTGACAGA GAAACTTTGT TAAATAGTGC AACCACTTCA 
CTGAACTCAA AGGTGGTTTC TCAGTATTCA AGTCTGCTTT CTCCAATGAG TGTAAATGCA 
GTGATGAAAG TGATTGACCC AGCCACAGCC ACCAGTGTAG ATCTTAGAGA TATTAAAATA 
GTTAAGAAGC TTGGTGGGAC AATTGATGAC TGTGAGTTGG TGGAAGGGCT GGTTCTCACC 
CAAAAAGTGT CAAATTCTGG CATAACCAGA GTTGAAAAGG CCAAGATTGG GCTTATTCAG 
TTTTGCTTAT CTGCTCCCAA AACAGACATG GATAATCAAA TAGTGGTTTC TGACTATGCC 
CAGATGGACC GAGTGCTGCG AGAAGAGAGA GCCTATATTT TAAATTTAGT GAAGCAAATT 
AAAAAAACAG GATGTAATGT CCTTCTCATA CAGAAATCTA TTCTAAGAGA TGCTCTTAGT 
GATCTTGCAT TACACTTTCT GAATAAAATG AAGATCATGG TGATTAAGGA TATTGAAAGA 
GAAGACATTG AATTCATTTG TAAGACAATT GGAACCAAGC CAGTTGCTCA TATTGACCAA 
TTTACTGCTG ACATGCTGGG TTCTGCTGAG TTAGCTGAGG AGGTCAATTT AAATGGTTCT 
GGCAAACTGC TCAAGATTAC AGGCTGTGCC AGCCCTGGAA AAACAGTTAC AATTGTTGTT 
CGTGGTTCTA ACAAACTGGT GATTGAAGAA GCTGAGCGCT CCATTCATGA TGCCCTATGT 
GTTATTCGTT GTTTAGTGAA GAAGAGGGCT CTTATTGCAG GAGGTGGTGC TCCAGAAATA 
GAGTTGGCCC TACGATTAAC TGAATATTCA CGAACACTGA GTGGTATGGA ATCCTACTGC 
GTTCGTGCTT TTGCAGATGC TATGGAGGTC ATTCCATCTA CACTAGCTGA AAATGCCGGC 
CTGAATCCCA TTTCTACAGT AACAGAACTA AGAAACCGGC ATGCCCAGGG AGAAAAAACT 
GCAGGCATTA ATGTCCGAAA GGGTGGTATT TCCAACATTT TGGAGGAACT GGTTGTCCAG 
CCTCTGTTGG TATCAGTCAG TGCTCTGACT CTTGCAACTG AAACTGTTCG GAGCATTCTG 
AAAATAGATG ATGTGGTAAA CACTCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteostatic control of telomerase function through TRiC-mediated folding of TCAB1.
Cell159(6)1389-403(2014 Dec)
Freund A,Zhong FL,Venteicher AS,Meng Z,Veenstra TD,Frydman J,Artandi SE

Chaperonin-containing TCP-1 complex directly binds to the cytoplasmic domain of the LOX-1 receptor.
FEBS letters588(13)2133-40(2014 Jun)
Bakthavatsalam D,Soung RH,Tweardy DJ,Chiu W,Dixon RA,Woodside DG

The chaperonin CCT interacts with and mediates the correct folding and activity of three subunits of translation initiation factor eIF3: b, i and h.
The Biochemical journal458(2)213-24(2014 Mar)
Roobol A,Roobol J,Carden MJ,Smith ME,Hershey JW,Bastide A,Knight JR,Willis AE,Smales CM

Human CCT4 and CCT5 chaperonin subunits expressed in Escherichia coli form biologically active homo-oligomers.
The Journal of biological chemistry288(24)17734-44(2013 Jun)
Sergeeva OA,Chen B,Haase-Pettingell C,Ludtke SJ,Chiu W,King JA

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CCT4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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