ARFGEF2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARFGEF2
Entrez Gene ID	10564
Full Name	ADP ribosylation factor guanine nucleotide exchange factor 2
Synonyms	BIG2,PVNH2,dJ1164I10.1
General protein information	Preferred Names ADP ribosylation factor guanine nucleotide exchange factor 2 Names brefeldin A-inhibited guanine nucleotide-exchange protein 2 ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) brefeldin A-inhibited GEP 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q13.13
Summary	ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq, Jul 2008].
Disorder MIM:	605371
Disorder Html:	Periventricular heterotopia with microcephaly, 608097 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_006420.2	NP_006411.2	brefeldin A-inhibited guanine nucleotide-exchange protein 2
XM_005260252.3	XP_005260309.1	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X1
NM_006420.3	NP_006411.2	brefeldin A-inhibited guanine nucleotide-exchange protein 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ARFGEF2	XP_534448.1
Arabidopsis thaliana (thale cress)	AT4G38200	NP_195533.2
Pan troglodytes (chimpanzee)	ARFGEF2	XP_001165517.2
	ARFGEF2	XP_002798173.1
Schizosaccharomyces pombe (fission yeast)	sec71	NP_594954.1
Arabidopsis thaliana (thale cress)	AT4G35380	NP_195264.2
Homo sapiens (human)	ARFGEF2	NP_006411.2
Rattus norvegicus (Norway rat)	Arfgef2	NP_851597.2
Danio rerio (zebrafish)	arfgef2	XP_002666513.2
Gallus gallus (chicken)	ARFGEF2	XP_417388.3
Xenopus tropicalis (tropical clawed frog)	arfgef2	XP_002933226.2
Bos taurus (cattle)	ARFGEF2	NP_001192581.1
Mus musculus (house mouse)	Arfgef2	NP_001078964.1

Gene Ontology
Bibliography

Related articles in PubMed

Enhancement of ?-catenin activity by BIG1 plus BIG2 via Arf activation and cAMP signals.
Li CC, Le K, Kato J, Moss J, Vaughan M
Proceedings of the National Academy of Sciences of the United States of America113(21)5946-51(2016 May)

Genome-wide association study of proneness to anger.
Mick E, McGough J, Deutsch CK, Frazier JA, Kennedy D, Goldberg RJ
PloS one9(1)e87257(2014)

West syndrome, microcephaly, grey matter heterotopia and hypoplasia of corpus callosum due to a novel ARFGEF2 mutation.
Banne E, Atawneh O, Henneke M, Brockmann K, G?rtner J, Elpeleg O, Edvardson S
Journal of medical genetics50(11)772-5(2013 Nov)

Elaborating the phenotypic spectrum associated with mutations in ARFGEF2: case study and literature review.
Tanyal?in I, Verhelst H, Halley DJ, Vanderhasselt T, Villard L, Goizet C, Lissens W, Mancini GM, Jansen AC
European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society17(6)666-70(2013 Nov)

Arf guanine nucleotide-exchange factors BIG1 and BIG2 regulate nonmuscle myosin IIA activity by anchoring myosin phosphatase complex.
Le K, Li CC, Ye G, Moss J, Vaughan M
Proceedings of the National Academy of Sciences of the United States of America110(34)E3162-70(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Both phospholipase D activity and vesicular trafficking were required for effects of BIG1 and BIG2 on beta-catenin activation. Levels of PKA-phosphorylated beta-catenin S675 and beta-catenin association with PKA, BIG1, and BIG2 were also diminished after BIG1/BIG2 depletion.
Title: Enhancement of ?-catenin activity by BIG1 plus BIG2 via Arf activation and cAMP signals.

Description of a novel ARFGEF2 mutation in five related patients presenting with West syndrome, microcephaly, periventricular heterotopia and thin corpus callosum.
Title: West syndrome, microcephaly, grey matter heterotopia and hypoplasia of corpus callosum due to a novel ARFGEF2 mutation.

The clinical phenotype associated with mutations in ARFGEF2 is relatively homogeneous in the families report.
Title: Elaborating the phenotypic spectrum associated with mutations in ARFGEF2: case study and literature review.

Arf guanine nucleotide-exchange factors BIG1 and BIG2 regulate nonmuscle myosin IIA activity by anchoring myosin phosphatase complex.
Title: Arf guanine nucleotide-exchange factors BIG1 and BIG2 regulate nonmuscle myosin IIA activity by anchoring myosin phosphatase complex.

an early acting GEF (GBF1) activates ARFs that mediate recruitment of late acting GEFs (BIG1/2) to coordinate coating events within the pre-Golgi/Golgi/TGN continuum.
Title: The Sec7 guanine nucleotide exchange factor GBF1 regulates membrane recruitment of BIG1 and BIG2 guanine nucleotide exchange factors to the trans-Golgi network (TGN).

The following ARFGEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARFGEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19545	NM_006420.3 Latest version!	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 2 (ARFGEF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu52862	XM_005260252.3 Latest version!	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 2 (ARFGEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OHu19545	NM_006420.2	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 2 (ARFGEF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARFGEF2 ( NM_006420.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_006411.2 Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 2 (ARFGEF2), mRNA.

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CloneID	OHu19545
Clone ID Related Accession (Same CDS sequence)	NM_006420.2 , NM_006420.3
Accession Version	NM_006420.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC050449.1, AK074303.1, AB209397.1 and AL121903.13. This sequence is a reference standard in the RefSeqGene project. On Jul 3, 2007 this sequence version replaced NM_006420.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.230691.1, AF084521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGGAGA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCC 
GACAAGGAGG TGAAGCGGCC CCAGCACTCC CAGCTGCGCA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAAAT AGAAAAGCAG AGGCTTGGCA CTGCTGCACC ACCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTTCTT CCATTCGAGC TAGCTTGCCA GTCCAAGTCC 
CCAAGGGTAG TCAGCACATC CCTTGACTGC TTGCAGAAAC TCATCGCATA CGGGCACATC 
ACTGGCAACG CCCCTGACAG TGGAGCCCCT GGGAAGCGGC TGATCGACAG AATTGTTGAA 
ACCATTTGCA GTTGTTTTCA GGGCCCTCAG ACTGATGAAG GGGTTCAGTT ACAAATAATT 
AAGGCTCTTC TGACTGCAGT GACTTCCCCA CACATTGAAA TTCATGAGGG TACTATCCTG 
CAGACAGTGA GAACATGTTA CAATATCTAT TTGGCCAGCA AAAATCTCAT CAATCAAACC 
ACTGCCAAGG CTACCCTTAC TCAGATGCTG AACGTCATTT TCACCCGCAT GGAAAACCAA 
GTGTTGCAGG AGGCCAGAGA ACTGGAAAAA CCAATCCAGT CAAAACCCCA GTCCCCTGTG 
ATCCAAGCTG CAGCAGTATC CCCAAAGTTC GTTCGTTTGA AGCACAGTCA GGCACAAAGC 
AAACCAACAA CTCCCGAAAA AACAGATTTA ACCAACGGTG AACATGCCAG GAGTGATTCT 
GGAAAAGTAA GCACAGAAAA TGGAGACGCA CCCAGAGAAA GAGGCTCATC ACTGTCAGGG 
ACTGATGACG GAGCCCAGGA GGTGGTGAAG GACATCTTGG AAGATGTAGT CACATCTGCC 
ATTAAAGAAG CAGCGGAAAA GCATGGTCTG ACAGAACCTG AGAGAGTTCT AGGTGAACTG 
GAGTGCCAGG AATGTGCTAT TCCCCCAGGA GTTGATGAAA ACTCACAGAC CAACGGGATA 
GCCGATGACA GGCAGTCCTT GTCGTCAGCA GATAATCTGG AATCGGATGC ACAAGGACAT 
CAAGTGGCTG CCAGGTTCTC CCACGTTCTG CAGAAGGATG CCTTCCTTGT GTTCCGCTCC 
CTGTGCAAGC TGTCCATGAA ACCCCTTGGT GAAGGCCCTC CAGACCCAAA ATCCCATGAG 
CTGCGTTCCA AGGTGGTTTC CCTGCAGCTG CTCCTCTCTG TGTTGCAAAA TGCTGGCCCC 
GTATTCAGGA CTCACGAGAT GTTCATCAAT GCAATCAAGC AATATCTCTG TGTGGCCTTG 
TCCAAAAACG GCGTCTCTTC AGTGCCTGAT GTCTTTGAGC TCTCTCTTGC CATTTTTCTT 
ACTCTTCTTT CAAACTTTAA AATGCACTTG AAAATGCAGA TAGAGGTCTT TTTCAAAGAG 
ATTTTCCTGA ACATTTTAGA AACATCAACA AGTTCTTTTG AGCACAGGTG GATGGTCATT 
CAGACTCTGA CGAGGATCTG TGCAGATGCC CAGTGTGTTG TGGATATTTA TGTCAACTAC 
GACTGTGATT TAAATGCTGC TAACATTTTT GAGCGCCTTG TAAATGATTT ATCCAAAATT 
GCTCAGGGAA GAAGTGGACA TGAGCTGGGA ATGACACCTC TGCAGGAGCT CAGCCTGAGG 
AAGAAAGGCC TGGAGTGCCT CGTGTCCATT CTCAAGTGCA TGGTGGAGTG GAGCAAAGAC 
CTGTATGTGA ATCCCAACCA CCAGACCAGC CTCGGTCAGG AGAGGCTCAC GGATCAGGAA 
ATAGGGGATG GGAAAGGCCT TGACATGGCA AGACGGTGTA GTGTGACGTC CATGGAGTCC 
ACAGTGTCCT CGGGGACCCA GACAACTGTT CAGGATGACC CTGAGCAATT TGAGGTCATC 
AAGCAACAAA AAGAAATCAT TGAACACGGC ATCGAGCTGT TCAACAAGAA ACCCAAGAGG 
GGGATCCAGT TTCTCCAGGA GCAGGGCATG CTGGGAACGT CAGTTGAAGA CATAGCCCAA 
TTCCTGCACC AGGAGGAGCG CCTGGATTCC ACCCAAGTAG GCGATTTTCT GGGAGATAGC 
GCAAGGTTCA ACAAGGAGGT GATGTATGCC TACGTGGACC AACTTGACTT CTGTGAAAAA 
GAATTTGTCT CAGCCCTGCG GACATTCCTA GAAGGTTTCC GCCTACCTGG AGAAGCCCAA 
AAGATTGACC GATTAATGGA GAAGTTTGCC GCAAGATACA TAGAATGCAA CCAAGGGCAA 
ACTCTGTTTG CTAGTGCTGA CACTGCTTAT GTCCTAGCGT ATTCAATTAT TATGCTGACT 
ACAGACTTGC ACAGTCCTCA GGTAAAAAAT AAAATGACGA AAGAGCAGTA TATTAAAATG 
AATCGGGGTA TCAATGATAG TAAAGATCTG CCAGAAGAGT ATCTCTCAAG CATCTATGAA 
GAGATAGAAG GCAAGAAAAT TGCAATGAAA GAAACAAAAG AGCTAACGAT TGCAACCAAA 
TCTACTAAGC AGAATGTAGC TAGTGAAAAG CAGCGGCGGC TGCTGTACAA CTTAGAGATG 
GAGCAAATGG CTAAAACAGC CAAAGCTCTG ATGGAGGCTG TGAGCCATGC CAAAGCCCCG 
TTTACCAGTG CCACTCACCT GGACCATGTC CGGCCAATGT TCAAACTGGT GTGGACGCCA 
CTATTGGCAG CCTACAGCAT CGGACTCCAG AACTGTGATG ACACTGAAGT GGCCTCCTTG 
TGTTTGGAAG GCATCCGATG TGCAATCCGA ATCGCCTGCA TCTTTGGAAT GCAGCTGGAA 
CGAGATGCCT ATGTTCAGGC TCTTGCTCGC TTCTCCCTAC TCACAGCCAG CTCCAGCATC 
ACAGAAATGA AGCAGAAAAA CATCGACACC ATTAAGACGC TTATCACAGT GGCTCACACC 
GATGGCAACT ACCTTGGGAA TTCCTGGCAT GAGATCTTGA AATGCATCAG CCAGCTGGAG 
CTCGCTCAGC TGATAGGAAC CGGTGTGAAG ACGCGCTACC TGTCTGGATC TGGGCGTGAA 
AGAGAAGGGA GCCTGAAGGG CCACACATTG GCAGGAGAAG AGTTCATGGG CCTTGGCCTC 
GGTAATTTGG TGAGTGGCGG AGTGGATAAA AGACAGATGG CCAGCTTCCA AGAATCGGTT 
GGTGAGACCA GCTCGCAGAG TGTGGTTGTA GCTGTGGACA GGATTTTTAC TGGGTCTACC 
AGACTGGATG GAAATGCAAT AGTTGACTTT GTCCGCTGGC TGTGTGCTGT GTCCATGGAT 
GAACTGGCTT CCCCCCACCA TCCTCGCATG TTCAGCTTGC AGAAGATTGT GGAGATATCA 
TACTACAACA TGAATCGGAT CCGACTACAG TGGTCTCGAA TATGGCATGT GATTGGAGAT 
CACTTCAATA AGGTTGGCTG CAACCCTAAT GAAGATGTGG CTATCTTTGC TGTTGACTCA 
TTAAGGCAAC TCTCCATGAA GTTTCTTGAG AAGGGTGAAT TAGCCAACTT CCGTTTCCAG 
AAAGATTTTC TGAGGCCCTT TGAGCATATT ATGAAGAAAA ACAGGTCTCC CACCATCCGG 
GACATGGCGA TCCGCTGCAT TGCCCAGATG GTGAACTCCC AGGCGGCCAA CATCCGCTCA 
GGTTGGAAGA ACATCTTTGC CGTGTTCCAC CAGGCAGCCT CTGATCATGA TGGGAACATT 
GTGGAGCTGG CCTTCCAGAC CACTTGCCAC ATTGTCACAA CTATTTTCCA GCACCATTTT 
CCTGCAGCCA TCGATTCCTT TCAGGATGCT GTGAAGTGCT TATCAGAGTT CGCCTGCAAC 
GCCGCTTTCC CTGACACGAG CATGGAAGCG ATTCGGCTCA TCCGCTTCTG TGGCAAATAC 
GTCTCTGAGA GGCCTCGGGT GCTACAAGAA TACACAAGTG ATGACATGAA TGTAGCTCCT 
GGTGACAGAG TCTGGGTCCG AGGCTGGTTC CCCATCTTAT TCGAACTCTC CTGCATCATT 
AATAGATGCA AGTTAGATGT ACGAACAAGG GGACTCACAG TCATGTTTGA GATCATGAAG 
AGCTATGGCC ACACCTTTGA AAAGCACTGG TGGCAGGACC TGTTCAGAAT CGTGTTTCGG 
ATTTTTGACA ATATGAAACT CCCTGAGCAA CTGTCAGAGA AATCTGAGTG GATGACAACA 
ACCTGCAATC ACGCACTTTA TGCTATTTGT GATGTTTTTA CCCAGTTTTA TGAAGCTTTG 
AATGAAGTTC TTCTTTCTGA TGTATTTGCA CAATTGCAGT GGTGTGTCAA ACAAGATAAT 
GAACAGTTGG CGCGATCAGG TACAAATTGC TTAGAAAACT TAGTAATATC CAATGGAGAG 
AAATTCAGTC CTGAAGTCTG GGATGAAACC TGCAACTGTA TGTTGGATAT TTTCAAAACA 
ACCATCCCAC ATGTTTTGCT GACATGGAGA CCTGTAGGAA TGGAGGAAGA TTCATCAGAA 
AAGCATTTGG ATGTGGATCT GGACCGCCAG TCTTTAAGCA GCATAGATAA AAATCCCTCT 
GAGAGGGGAC AGAGCCAGCT CTCTAACCCA ACAGATGACA GCTGGAAGGG TAGACCATAC 
GCAAATCAGA AACTGTTTGC CAGCCTCCTC ATCAAGTGTG TGGTCCAGTT GGAATTGATA 
CAGACCATTG ACAACATTGT GTTCTACCCT GCGACGAGCA AAAAGGAGGA TGCAGAGCAC 
ATGGTTGCCG CCCAGCAAGA CACGCTGGAT GCAGATATCC ACATAGAGAC GGAGGATCAG 
GGCATGTATA AGTACATGTC TTCCCAGCAC CTCTTCAAGC TGTTGGACTG TTTGCAGGAA 
TCCCATTCAT TCTCAAAGGC CTTCAACTCC AATTACGAGC AGCGGACTGT CCTGTGGCGA 
GCAGGTTTTA AGGGCAAGTC TAAACCCAAT CTTCTAAAAC AAGAAACCAG CAGCCTGGCC 
TGTTGTTTGA GGATCCTGTT TCGAATGTAT GTTGATGAGA ACCGCAGGGA TTCCTGGGAA 
GAAATACAGC AGAGACTTTT AACTGTTTGC AGTGAAGCTC TTGCCTATTT CATCACTGTG 
AATTCTGAGA GCCATCGGGA GGCCTGGACA AGTCTCTTGT TGTTACTTCT AACTAAAACC 
CTCAAAATAA ATGATGAAAA GTTCAAAGCA CATGCTTCAA TGTACTACCC CTACTTGTGT 
GAAATTATGC AGTTTGACCT GATCCCTGAG CTCCGAGCAG TTCTGCGGAA GTTCTTCCTA 
CGGATAGGTG TTGTGTATAA GATATGGATA CCAGAAGAGC CATCACAGGT ACCAGCAGCA 
CTGTCACCAG TGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006411.2
CDS	153..5510
Translation	MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEIEKQRLGTAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICSCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPIQSKPQSPVIQAAAVSPKFVRLKHSQAQSKPTTPEKTDLTNGEHARSDSGKVSTENGDAPRERGSSLSGTDDGAQEVVKDILEDVVTSAIKEAAEKHGLTEPERVLGELECQECAIPPGVDENSQTNGIADDRQSLSSADNLESDAQGHQVAARFSHVLQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRTHEMFINAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQTSLGQERLTDQEIGDGKGLDMARRCSVTSMESTVSSGTQTTVQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTSVEDIAQFLHQEERLDSTQVGDFLGDSARFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKELTIATKSTKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAIRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHTLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMAIRCIAQMVNSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTCHIVTTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWQDLFRIVFRIFDNMKLPEQLSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPEVWDETCNCMLDIFKTTIPHVLLTWRPVGMEEDSSEKHLDVDLDRQSLSSIDKNPSERGQSQLSNPTDDSWKGRPYANQKLFASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETEDQGMYKYMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWEEIQQRLLTVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKINDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGVVYKIWIPEEPSQVPAALSPVW

Target ORF information:

RefSeq Version	NM_006420.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 2 (ARFGEF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006420.2

ORF Insert Sequence:

ATGCAGGAGA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCC 
GACAAGGAGG TGAAGCGGCC CCAGCACTCC CAGCTGCGCA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAAAT AGAAAAGCAG AGGCTTGGCA CTGCTGCACC ACCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTTCTT CCATTCGAGC TAGCTTGCCA GTCCAAGTCC 
CCAAGGGTAG TCAGCACATC CCTTGACTGC TTGCAGAAAC TCATCGCATA CGGGCACATC 
ACTGGCAACG CCCCTGACAG TGGAGCCCCT GGGAAGCGGC TGATCGACAG AATTGTTGAA 
ACCATTTGCA GTTGTTTTCA GGGCCCTCAG ACTGATGAAG GGGTTCAGTT ACAAATAATT 
AAGGCTCTTC TGACTGCAGT GACTTCCCCA CACATTGAAA TTCATGAGGG TACTATCCTG 
CAGACAGTGA GAACATGTTA CAATATCTAT TTGGCCAGCA AAAATCTCAT CAATCAAACC 
ACTGCCAAGG CTACCCTTAC TCAGATGCTG AACGTCATTT TCACCCGCAT GGAAAACCAA 
GTGTTGCAGG AGGCCAGAGA ACTGGAAAAA CCAATCCAGT CAAAACCCCA GTCCCCTGTG 
ATCCAAGCTG CAGCAGTATC CCCAAAGTTC GTTCGTTTGA AGCACAGTCA GGCACAAAGC 
AAACCAACAA CTCCCGAAAA AACAGATTTA ACCAACGGTG AACATGCCAG GAGTGATTCT 
GGAAAAGTAA GCACAGAAAA TGGAGACGCA CCCAGAGAAA GAGGCTCATC ACTGTCAGGG 
ACTGATGACG GAGCCCAGGA GGTGGTGAAG GACATCTTGG AAGATGTAGT CACATCTGCC 
ATTAAAGAAG CAGCGGAAAA GCATGGTCTG ACAGAACCTG AGAGAGTTCT AGGTGAACTG 
GAGTGCCAGG AATGTGCTAT TCCCCCAGGA GTTGATGAAA ACTCACAGAC CAACGGGATA 
GCCGATGACA GGCAGTCCTT GTCGTCAGCA GATAATCTGG AATCGGATGC ACAAGGACAT 
CAAGTGGCTG CCAGGTTCTC CCACGTTCTG CAGAAGGATG CCTTCCTTGT GTTCCGCTCC 
CTGTGCAAGC TGTCCATGAA ACCCCTTGGT GAAGGCCCTC CAGACCCAAA ATCCCATGAG 
CTGCGTTCCA AGGTGGTTTC CCTGCAGCTG CTCCTCTCTG TGTTGCAAAA TGCTGGCCCC 
GTATTCAGGA CTCACGAGAT GTTCATCAAT GCAATCAAGC AATATCTCTG TGTGGCCTTG 
TCCAAAAACG GCGTCTCTTC AGTGCCTGAT GTCTTTGAGC TCTCTCTTGC CATTTTTCTT 
ACTCTTCTTT CAAACTTTAA AATGCACTTG AAAATGCAGA TAGAGGTCTT TTTCAAAGAG 
ATTTTCCTGA ACATTTTAGA AACATCAACA AGTTCTTTTG AGCACAGGTG GATGGTCATT 
CAGACTCTGA CGAGGATCTG TGCAGATGCC CAGTGTGTTG TGGATATTTA TGTCAACTAC 
GACTGTGATT TAAATGCTGC TAACATTTTT GAGCGCCTTG TAAATGATTT ATCCAAAATT 
GCTCAGGGAA GAAGTGGACA TGAGCTGGGA ATGACACCTC TGCAGGAGCT CAGCCTGAGG 
AAGAAAGGCC TGGAGTGCCT CGTGTCCATT CTCAAGTGCA TGGTGGAGTG GAGCAAAGAC 
CTGTATGTGA ATCCCAACCA CCAGACCAGC CTCGGTCAGG AGAGGCTCAC GGATCAGGAA 
ATAGGGGATG GGAAAGGCCT TGACATGGCA AGACGGTGTA GTGTGACGTC CATGGAGTCC 
ACAGTGTCCT CGGGGACCCA GACAACTGTT CAGGATGACC CTGAGCAATT TGAGGTCATC 
AAGCAACAAA AAGAAATCAT TGAACACGGC ATCGAGCTGT TCAACAAGAA ACCCAAGAGG 
GGGATCCAGT TTCTCCAGGA GCAGGGCATG CTGGGAACGT CAGTTGAAGA CATAGCCCAA 
TTCCTGCACC AGGAGGAGCG CCTGGATTCC ACCCAAGTAG GCGATTTTCT GGGAGATAGC 
GCAAGGTTCA ACAAGGAGGT GATGTATGCC TACGTGGACC AACTTGACTT CTGTGAAAAA 
GAATTTGTCT CAGCCCTGCG GACATTCCTA GAAGGTTTCC GCCTACCTGG AGAAGCCCAA 
AAGATTGACC GATTAATGGA GAAGTTTGCC GCAAGATACA TAGAATGCAA CCAAGGGCAA 
ACTCTGTTTG CTAGTGCTGA CACTGCTTAT GTCCTAGCGT ATTCAATTAT TATGCTGACT 
ACAGACTTGC ACAGTCCTCA GGTAAAAAAT AAAATGACGA AAGAGCAGTA TATTAAAATG 
AATCGGGGTA TCAATGATAG TAAAGATCTG CCAGAAGAGT ATCTCTCAAG CATCTATGAA 
GAGATAGAAG GCAAGAAAAT TGCAATGAAA GAAACAAAAG AGCTAACGAT TGCAACCAAA 
TCTACTAAGC AGAATGTAGC TAGTGAAAAG CAGCGGCGGC TGCTGTACAA CTTAGAGATG 
GAGCAAATGG CTAAAACAGC CAAAGCTCTG ATGGAGGCTG TGAGCCATGC CAAAGCCCCG 
TTTACCAGTG CCACTCACCT GGACCATGTC CGGCCAATGT TCAAACTGGT GTGGACGCCA 
CTATTGGCAG CCTACAGCAT CGGACTCCAG AACTGTGATG ACACTGAAGT GGCCTCCTTG 
TGTTTGGAAG GCATCCGATG TGCAATCCGA ATCGCCTGCA TCTTTGGAAT GCAGCTGGAA 
CGAGATGCCT ATGTTCAGGC TCTTGCTCGC TTCTCCCTAC TCACAGCCAG CTCCAGCATC 
ACAGAAATGA AGCAGAAAAA CATCGACACC ATTAAGACGC TTATCACAGT GGCTCACACC 
GATGGCAACT ACCTTGGGAA TTCCTGGCAT GAGATCTTGA AATGCATCAG CCAGCTGGAG 
CTCGCTCAGC TGATAGGAAC CGGTGTGAAG ACGCGCTACC TGTCTGGATC TGGGCGTGAA 
AGAGAAGGGA GCCTGAAGGG CCACACATTG GCAGGAGAAG AGTTCATGGG CCTTGGCCTC 
GGTAATTTGG TGAGTGGCGG AGTGGATAAA AGACAGATGG CCAGCTTCCA AGAATCGGTT 
GGTGAGACCA GCTCGCAGAG TGTGGTTGTA GCTGTGGACA GGATTTTTAC TGGGTCTACC 
AGACTGGATG GAAATGCAAT AGTTGACTTT GTCCGCTGGC TGTGTGCTGT GTCCATGGAT 
GAACTGGCTT CCCCCCACCA TCCTCGCATG TTCAGCTTGC AGAAGATTGT GGAGATATCA 
TACTACAACA TGAATCGGAT CCGACTACAG TGGTCTCGAA TATGGCATGT GATTGGAGAT 
CACTTCAATA AGGTTGGCTG CAACCCTAAT GAAGATGTGG CTATCTTTGC TGTTGACTCA 
TTAAGGCAAC TCTCCATGAA GTTTCTTGAG AAGGGTGAAT TAGCCAACTT CCGTTTCCAG 
AAAGATTTTC TGAGGCCCTT TGAGCATATT ATGAAGAAAA ACAGGTCTCC CACCATCCGG 
GACATGGCGA TCCGCTGCAT TGCCCAGATG GTGAACTCCC AGGCGGCCAA CATCCGCTCA 
GGTTGGAAGA ACATCTTTGC CGTGTTCCAC CAGGCAGCCT CTGATCATGA TGGGAACATT 
GTGGAGCTGG CCTTCCAGAC CACTTGCCAC ATTGTCACAA CTATTTTCCA GCACCATTTT 
CCTGCAGCCA TCGATTCCTT TCAGGATGCT GTGAAGTGCT TATCAGAGTT CGCCTGCAAC 
GCCGCTTTCC CTGACACGAG CATGGAAGCG ATTCGGCTCA TCCGCTTCTG TGGCAAATAC 
GTCTCTGAGA GGCCTCGGGT GCTACAAGAA TACACAAGTG ATGACATGAA TGTAGCTCCT 
GGTGACAGAG TCTGGGTCCG AGGCTGGTTC CCCATCTTAT TCGAACTCTC CTGCATCATT 
AATAGATGCA AGTTAGATGT ACGAACAAGG GGACTCACAG TCATGTTTGA GATCATGAAG 
AGCTATGGCC ACACCTTTGA AAAGCACTGG TGGCAGGACC TGTTCAGAAT CGTGTTTCGG 
ATTTTTGACA ATATGAAACT CCCTGAGCAA CTGTCAGAGA AATCTGAGTG GATGACAACA 
ACCTGCAATC ACGCACTTTA TGCTATTTGT GATGTTTTTA CCCAGTTTTA TGAAGCTTTG 
AATGAAGTTC TTCTTTCTGA TGTATTTGCA CAATTGCAGT GGTGTGTCAA ACAAGATAAT 
GAACAGTTGG CGCGATCAGG TACAAATTGC TTAGAAAACT TAGTAATATC CAATGGAGAG 
AAATTCAGTC CTGAAGTCTG GGATGAAACC TGCAACTGTA TGTTGGATAT TTTCAAAACA 
ACCATCCCAC ATGTTTTGCT GACATGGAGA CCTGTAGGAA TGGAGGAAGA TTCATCAGAA 
AAGCATTTGG ATGTGGATCT GGACCGCCAG TCTTTAAGCA GCATAGATAA AAATCCCTCT 
GAGAGGGGAC AGAGCCAGCT CTCTAACCCA ACAGATGACA GCTGGAAGGG TAGACCATAC 
GCAAATCAGA AACTGTTTGC CAGCCTCCTC ATCAAGTGTG TGGTCCAGTT GGAATTGATA 
CAGACCATTG ACAACATTGT GTTCTACCCT GCGACGAGCA AAAAGGAGGA TGCAGAGCAC 
ATGGTTGCCG CCCAGCAAGA CACGCTGGAT GCAGATATCC ACATAGAGAC GGAGGATCAG 
GGCATGTATA AGTACATGTC TTCCCAGCAC CTCTTCAAGC TGTTGGACTG TTTGCAGGAA 
TCCCATTCAT TCTCAAAGGC CTTCAACTCC AATTACGAGC AGCGGACTGT CCTGTGGCGA 
GCAGGTTTTA AGGGCAAGTC TAAACCCAAT CTTCTAAAAC AAGAAACCAG CAGCCTGGCC 
TGTTGTTTGA GGATCCTGTT TCGAATGTAT GTTGATGAGA ACCGCAGGGA TTCCTGGGAA 
GAAATACAGC AGAGACTTTT AACTGTTTGC AGTGAAGCTC TTGCCTATTT CATCACTGTG 
AATTCTGAGA GCCATCGGGA GGCCTGGACA AGTCTCTTGT TGTTACTTCT AACTAAAACC 
CTCAAAATAA ATGATGAAAA GTTCAAAGCA CATGCTTCAA TGTACTACCC CTACTTGTGT 
GAAATTATGC AGTTTGACCT GATCCCTGAG CTCCGAGCAG TTCTGCGGAA GTTCTTCCTA 
CGGATAGGTG TTGTGTATAA GATATGGATA CCAGAAGAGC CATCACAGGT ACCAGCAGCA 
CTGTCACCAG TGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARFGEF2 ( NM_006420.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006411.2 Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 2 (ARFGEF2), mRNA.

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CloneID	OHu19545
Clone ID Related Accession (Same CDS sequence)	NM_006420.2 , NM_006420.3
Accession Version	NM_006420.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL049537.48, BC050449.1, AK074303.1, AB209397.1 and AL121903.13. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_006420.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.230691.1, AF084521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000371917.5/ ENSP00000360985.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGGAGA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCC 
GACAAGGAGG TGAAGCGGCC CCAGCACTCC CAGCTGCGCA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAAAT AGAAAAGCAG AGGCTTGGCA CTGCTGCACC ACCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTTCTT CCATTCGAGC TAGCTTGCCA GTCCAAGTCC 
CCAAGGGTAG TCAGCACATC CCTTGACTGC TTGCAGAAAC TCATCGCATA CGGGCACATC 
ACTGGCAACG CCCCTGACAG TGGAGCCCCT GGGAAGCGGC TGATCGACAG AATTGTTGAA 
ACCATTTGCA GTTGTTTTCA GGGCCCTCAG ACTGATGAAG GGGTTCAGTT ACAAATAATT 
AAGGCTCTTC TGACTGCAGT GACTTCCCCA CACATTGAAA TTCATGAGGG TACTATCCTG 
CAGACAGTGA GAACATGTTA CAATATCTAT TTGGCCAGCA AAAATCTCAT CAATCAAACC 
ACTGCCAAGG CTACCCTTAC TCAGATGCTG AACGTCATTT TCACCCGCAT GGAAAACCAA 
GTGTTGCAGG AGGCCAGAGA ACTGGAAAAA CCAATCCAGT CAAAACCCCA GTCCCCTGTG 
ATCCAAGCTG CAGCAGTATC CCCAAAGTTC GTTCGTTTGA AGCACAGTCA GGCACAAAGC 
AAACCAACAA CTCCCGAAAA AACAGATTTA ACCAACGGTG AACATGCCAG GAGTGATTCT 
GGAAAAGTAA GCACAGAAAA TGGAGACGCA CCCAGAGAAA GAGGCTCATC ACTGTCAGGG 
ACTGATGACG GAGCCCAGGA GGTGGTGAAG GACATCTTGG AAGATGTAGT CACATCTGCC 
ATTAAAGAAG CAGCGGAAAA GCATGGTCTG ACAGAACCTG AGAGAGTTCT AGGTGAACTG 
GAGTGCCAGG AATGTGCTAT TCCCCCAGGA GTTGATGAAA ACTCACAGAC CAACGGGATA 
GCCGATGACA GGCAGTCCTT GTCGTCAGCA GATAATCTGG AATCGGATGC ACAAGGACAT 
CAAGTGGCTG CCAGGTTCTC CCACGTTCTG CAGAAGGATG CCTTCCTTGT GTTCCGCTCC 
CTGTGCAAGC TGTCCATGAA ACCCCTTGGT GAAGGCCCTC CAGACCCAAA ATCCCATGAG 
CTGCGTTCCA AGGTGGTTTC CCTGCAGCTG CTCCTCTCTG TGTTGCAAAA TGCTGGCCCC 
GTATTCAGGA CTCACGAGAT GTTCATCAAT GCAATCAAGC AATATCTCTG TGTGGCCTTG 
TCCAAAAACG GCGTCTCTTC AGTGCCTGAT GTCTTTGAGC TCTCTCTTGC CATTTTTCTT 
ACTCTTCTTT CAAACTTTAA AATGCACTTG AAAATGCAGA TAGAGGTCTT TTTCAAAGAG 
ATTTTCCTGA ACATTTTAGA AACATCAACA AGTTCTTTTG AGCACAGGTG GATGGTCATT 
CAGACTCTGA CGAGGATCTG TGCAGATGCC CAGTGTGTTG TGGATATTTA TGTCAACTAC 
GACTGTGATT TAAATGCTGC TAACATTTTT GAGCGCCTTG TAAATGATTT ATCCAAAATT 
GCTCAGGGAA GAAGTGGACA TGAGCTGGGA ATGACACCTC TGCAGGAGCT CAGCCTGAGG 
AAGAAAGGCC TGGAGTGCCT CGTGTCCATT CTCAAGTGCA TGGTGGAGTG GAGCAAAGAC 
CTGTATGTGA ATCCCAACCA CCAGACCAGC CTCGGTCAGG AGAGGCTCAC GGATCAGGAA 
ATAGGGGATG GGAAAGGCCT TGACATGGCA AGACGGTGTA GTGTGACGTC CATGGAGTCC 
ACAGTGTCCT CGGGGACCCA GACAACTGTT CAGGATGACC CTGAGCAATT TGAGGTCATC 
AAGCAACAAA AAGAAATCAT TGAACACGGC ATCGAGCTGT TCAACAAGAA ACCCAAGAGG 
GGGATCCAGT TTCTCCAGGA GCAGGGCATG CTGGGAACGT CAGTTGAAGA CATAGCCCAA 
TTCCTGCACC AGGAGGAGCG CCTGGATTCC ACCCAAGTAG GCGATTTTCT GGGAGATAGC 
GCAAGGTTCA ACAAGGAGGT GATGTATGCC TACGTGGACC AACTTGACTT CTGTGAAAAA 
GAATTTGTCT CAGCCCTGCG GACATTCCTA GAAGGTTTCC GCCTACCTGG AGAAGCCCAA 
AAGATTGACC GATTAATGGA GAAGTTTGCC GCAAGATACA TAGAATGCAA CCAAGGGCAA 
ACTCTGTTTG CTAGTGCTGA CACTGCTTAT GTCCTAGCGT ATTCAATTAT TATGCTGACT 
ACAGACTTGC ACAGTCCTCA GGTAAAAAAT AAAATGACGA AAGAGCAGTA TATTAAAATG 
AATCGGGGTA TCAATGATAG TAAAGATCTG CCAGAAGAGT ATCTCTCAAG CATCTATGAA 
GAGATAGAAG GCAAGAAAAT TGCAATGAAA GAAACAAAAG AGCTAACGAT TGCAACCAAA 
TCTACTAAGC AGAATGTAGC TAGTGAAAAG CAGCGGCGGC TGCTGTACAA CTTAGAGATG 
GAGCAAATGG CTAAAACAGC CAAAGCTCTG ATGGAGGCTG TGAGCCATGC CAAAGCCCCG 
TTTACCAGTG CCACTCACCT GGACCATGTC CGGCCAATGT TCAAACTGGT GTGGACGCCA 
CTATTGGCAG CCTACAGCAT CGGACTCCAG AACTGTGATG ACACTGAAGT GGCCTCCTTG 
TGTTTGGAAG GCATCCGATG TGCAATCCGA ATCGCCTGCA TCTTTGGAAT GCAGCTGGAA 
CGAGATGCCT ATGTTCAGGC TCTTGCTCGC TTCTCCCTAC TCACAGCCAG CTCCAGCATC 
ACAGAAATGA AGCAGAAAAA CATCGACACC ATTAAGACGC TTATCACAGT GGCTCACACC 
GATGGCAACT ACCTTGGGAA TTCCTGGCAT GAGATCTTGA AATGCATCAG CCAGCTGGAG 
CTCGCTCAGC TGATAGGAAC CGGTGTGAAG ACGCGCTACC TGTCTGGATC TGGGCGTGAA 
AGAGAAGGGA GCCTGAAGGG CCACACATTG GCAGGAGAAG AGTTCATGGG CCTTGGCCTC 
GGTAATTTGG TGAGTGGCGG AGTGGATAAA AGACAGATGG CCAGCTTCCA AGAATCGGTT 
GGTGAGACCA GCTCGCAGAG TGTGGTTGTA GCTGTGGACA GGATTTTTAC TGGGTCTACC 
AGACTGGATG GAAATGCAAT AGTTGACTTT GTCCGCTGGC TGTGTGCTGT GTCCATGGAT 
GAACTGGCTT CCCCCCACCA TCCTCGCATG TTCAGCTTGC AGAAGATTGT GGAGATATCA 
TACTACAACA TGAATCGGAT CCGACTACAG TGGTCTCGAA TATGGCATGT GATTGGAGAT 
CACTTCAATA AGGTTGGCTG CAACCCTAAT GAAGATGTGG CTATCTTTGC TGTTGACTCA 
TTAAGGCAAC TCTCCATGAA GTTTCTTGAG AAGGGTGAAT TAGCCAACTT CCGTTTCCAG 
AAAGATTTTC TGAGGCCCTT TGAGCATATT ATGAAGAAAA ACAGGTCTCC CACCATCCGG 
GACATGGCGA TCCGCTGCAT TGCCCAGATG GTGAACTCCC AGGCGGCCAA CATCCGCTCA 
GGTTGGAAGA ACATCTTTGC CGTGTTCCAC CAGGCAGCCT CTGATCATGA TGGGAACATT 
GTGGAGCTGG CCTTCCAGAC CACTTGCCAC ATTGTCACAA CTATTTTCCA GCACCATTTT 
CCTGCAGCCA TCGATTCCTT TCAGGATGCT GTGAAGTGCT TATCAGAGTT CGCCTGCAAC 
GCCGCTTTCC CTGACACGAG CATGGAAGCG ATTCGGCTCA TCCGCTTCTG TGGCAAATAC 
GTCTCTGAGA GGCCTCGGGT GCTACAAGAA TACACAAGTG ATGACATGAA TGTAGCTCCT 
GGTGACAGAG TCTGGGTCCG AGGCTGGTTC CCCATCTTAT TCGAACTCTC CTGCATCATT 
AATAGATGCA AGTTAGATGT ACGAACAAGG GGACTCACAG TCATGTTTGA GATCATGAAG 
AGCTATGGCC ACACCTTTGA AAAGCACTGG TGGCAGGACC TGTTCAGAAT CGTGTTTCGG 
ATTTTTGACA ATATGAAACT CCCTGAGCAA CTGTCAGAGA AATCTGAGTG GATGACAACA 
ACCTGCAATC ACGCACTTTA TGCTATTTGT GATGTTTTTA CCCAGTTTTA TGAAGCTTTG 
AATGAAGTTC TTCTTTCTGA TGTATTTGCA CAATTGCAGT GGTGTGTCAA ACAAGATAAT 
GAACAGTTGG CGCGATCAGG TACAAATTGC TTAGAAAACT TAGTAATATC CAATGGAGAG 
AAATTCAGTC CTGAAGTCTG GGATGAAACC TGCAACTGTA TGTTGGATAT TTTCAAAACA 
ACCATCCCAC ATGTTTTGCT GACATGGAGA CCTGTAGGAA TGGAGGAAGA TTCATCAGAA 
AAGCATTTGG ATGTGGATCT GGACCGCCAG TCTTTAAGCA GCATAGATAA AAATCCCTCT 
GAGAGGGGAC AGAGCCAGCT CTCTAACCCA ACAGATGACA GCTGGAAGGG TAGACCATAC 
GCAAATCAGA AACTGTTTGC CAGCCTCCTC ATCAAGTGTG TGGTCCAGTT GGAATTGATA 
CAGACCATTG ACAACATTGT GTTCTACCCT GCGACGAGCA AAAAGGAGGA TGCAGAGCAC 
ATGGTTGCCG CCCAGCAAGA CACGCTGGAT GCAGATATCC ACATAGAGAC GGAGGATCAG 
GGCATGTATA AGTACATGTC TTCCCAGCAC CTCTTCAAGC TGTTGGACTG TTTGCAGGAA 
TCCCATTCAT TCTCAAAGGC CTTCAACTCC AATTACGAGC AGCGGACTGT CCTGTGGCGA 
GCAGGTTTTA AGGGCAAGTC TAAACCCAAT CTTCTAAAAC AAGAAACCAG CAGCCTGGCC 
TGTTGTTTGA GGATCCTGTT TCGAATGTAT GTTGATGAGA ACCGCAGGGA TTCCTGGGAA 
GAAATACAGC AGAGACTTTT AACTGTTTGC AGTGAAGCTC TTGCCTATTT CATCACTGTG 
AATTCTGAGA GCCATCGGGA GGCCTGGACA AGTCTCTTGT TGTTACTTCT AACTAAAACC 
CTCAAAATAA ATGATGAAAA GTTCAAAGCA CATGCTTCAA TGTACTACCC CTACTTGTGT 
GAAATTATGC AGTTTGACCT GATCCCTGAG CTCCGAGCAG TTCTGCGGAA GTTCTTCCTA 
CGGATAGGTG TTGTGTATAA GATATGGATA CCAGAAGAGC CATCACAGGT ACCAGCAGCA 
CTGTCACCAG TGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006411.2
CDS	180..5537
Translation	MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEIEKQRLGTAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICSCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPIQSKPQSPVIQAAAVSPKFVRLKHSQAQSKPTTPEKTDLTNGEHARSDSGKVSTENGDAPRERGSSLSGTDDGAQEVVKDILEDVVTSAIKEAAEKHGLTEPERVLGELECQECAIPPGVDENSQTNGIADDRQSLSSADNLESDAQGHQVAARFSHVLQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRTHEMFINAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQTSLGQERLTDQEIGDGKGLDMARRCSVTSMESTVSSGTQTTVQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTSVEDIAQFLHQEERLDSTQVGDFLGDSARFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKELTIATKSTKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAIRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHTLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMAIRCIAQMVNSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTCHIVTTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWQDLFRIVFRIFDNMKLPEQLSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPEVWDETCNCMLDIFKTTIPHVLLTWRPVGMEEDSSEKHLDVDLDRQSLSSIDKNPSERGQSQLSNPTDDSWKGRPYANQKLFASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETEDQGMYKYMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWEEIQQRLLTVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKINDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGVVYKIWIPEEPSQVPAALSPVW

Target ORF information:

RefSeq Version	NM_006420.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 2 (ARFGEF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006420.3

ORF Insert Sequence:

ATGCAGGAGA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCC 
GACAAGGAGG TGAAGCGGCC CCAGCACTCC CAGCTGCGCA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAAAT AGAAAAGCAG AGGCTTGGCA CTGCTGCACC ACCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTTCTT CCATTCGAGC TAGCTTGCCA GTCCAAGTCC 
CCAAGGGTAG TCAGCACATC CCTTGACTGC TTGCAGAAAC TCATCGCATA CGGGCACATC 
ACTGGCAACG CCCCTGACAG TGGAGCCCCT GGGAAGCGGC TGATCGACAG AATTGTTGAA 
ACCATTTGCA GTTGTTTTCA GGGCCCTCAG ACTGATGAAG GGGTTCAGTT ACAAATAATT 
AAGGCTCTTC TGACTGCAGT GACTTCCCCA CACATTGAAA TTCATGAGGG TACTATCCTG 
CAGACAGTGA GAACATGTTA CAATATCTAT TTGGCCAGCA AAAATCTCAT CAATCAAACC 
ACTGCCAAGG CTACCCTTAC TCAGATGCTG AACGTCATTT TCACCCGCAT GGAAAACCAA 
GTGTTGCAGG AGGCCAGAGA ACTGGAAAAA CCAATCCAGT CAAAACCCCA GTCCCCTGTG 
ATCCAAGCTG CAGCAGTATC CCCAAAGTTC GTTCGTTTGA AGCACAGTCA GGCACAAAGC 
AAACCAACAA CTCCCGAAAA AACAGATTTA ACCAACGGTG AACATGCCAG GAGTGATTCT 
GGAAAAGTAA GCACAGAAAA TGGAGACGCA CCCAGAGAAA GAGGCTCATC ACTGTCAGGG 
ACTGATGACG GAGCCCAGGA GGTGGTGAAG GACATCTTGG AAGATGTAGT CACATCTGCC 
ATTAAAGAAG CAGCGGAAAA GCATGGTCTG ACAGAACCTG AGAGAGTTCT AGGTGAACTG 
GAGTGCCAGG AATGTGCTAT TCCCCCAGGA GTTGATGAAA ACTCACAGAC CAACGGGATA 
GCCGATGACA GGCAGTCCTT GTCGTCAGCA GATAATCTGG AATCGGATGC ACAAGGACAT 
CAAGTGGCTG CCAGGTTCTC CCACGTTCTG CAGAAGGATG CCTTCCTTGT GTTCCGCTCC 
CTGTGCAAGC TGTCCATGAA ACCCCTTGGT GAAGGCCCTC CAGACCCAAA ATCCCATGAG 
CTGCGTTCCA AGGTGGTTTC CCTGCAGCTG CTCCTCTCTG TGTTGCAAAA TGCTGGCCCC 
GTATTCAGGA CTCACGAGAT GTTCATCAAT GCAATCAAGC AATATCTCTG TGTGGCCTTG 
TCCAAAAACG GCGTCTCTTC AGTGCCTGAT GTCTTTGAGC TCTCTCTTGC CATTTTTCTT 
ACTCTTCTTT CAAACTTTAA AATGCACTTG AAAATGCAGA TAGAGGTCTT TTTCAAAGAG 
ATTTTCCTGA ACATTTTAGA AACATCAACA AGTTCTTTTG AGCACAGGTG GATGGTCATT 
CAGACTCTGA CGAGGATCTG TGCAGATGCC CAGTGTGTTG TGGATATTTA TGTCAACTAC 
GACTGTGATT TAAATGCTGC TAACATTTTT GAGCGCCTTG TAAATGATTT ATCCAAAATT 
GCTCAGGGAA GAAGTGGACA TGAGCTGGGA ATGACACCTC TGCAGGAGCT CAGCCTGAGG 
AAGAAAGGCC TGGAGTGCCT CGTGTCCATT CTCAAGTGCA TGGTGGAGTG GAGCAAAGAC 
CTGTATGTGA ATCCCAACCA CCAGACCAGC CTCGGTCAGG AGAGGCTCAC GGATCAGGAA 
ATAGGGGATG GGAAAGGCCT TGACATGGCA AGACGGTGTA GTGTGACGTC CATGGAGTCC 
ACAGTGTCCT CGGGGACCCA GACAACTGTT CAGGATGACC CTGAGCAATT TGAGGTCATC 
AAGCAACAAA AAGAAATCAT TGAACACGGC ATCGAGCTGT TCAACAAGAA ACCCAAGAGG 
GGGATCCAGT TTCTCCAGGA GCAGGGCATG CTGGGAACGT CAGTTGAAGA CATAGCCCAA 
TTCCTGCACC AGGAGGAGCG CCTGGATTCC ACCCAAGTAG GCGATTTTCT GGGAGATAGC 
GCAAGGTTCA ACAAGGAGGT GATGTATGCC TACGTGGACC AACTTGACTT CTGTGAAAAA 
GAATTTGTCT CAGCCCTGCG GACATTCCTA GAAGGTTTCC GCCTACCTGG AGAAGCCCAA 
AAGATTGACC GATTAATGGA GAAGTTTGCC GCAAGATACA TAGAATGCAA CCAAGGGCAA 
ACTCTGTTTG CTAGTGCTGA CACTGCTTAT GTCCTAGCGT ATTCAATTAT TATGCTGACT 
ACAGACTTGC ACAGTCCTCA GGTAAAAAAT AAAATGACGA AAGAGCAGTA TATTAAAATG 
AATCGGGGTA TCAATGATAG TAAAGATCTG CCAGAAGAGT ATCTCTCAAG CATCTATGAA 
GAGATAGAAG GCAAGAAAAT TGCAATGAAA GAAACAAAAG AGCTAACGAT TGCAACCAAA 
TCTACTAAGC AGAATGTAGC TAGTGAAAAG CAGCGGCGGC TGCTGTACAA CTTAGAGATG 
GAGCAAATGG CTAAAACAGC CAAAGCTCTG ATGGAGGCTG TGAGCCATGC CAAAGCCCCG 
TTTACCAGTG CCACTCACCT GGACCATGTC CGGCCAATGT TCAAACTGGT GTGGACGCCA 
CTATTGGCAG CCTACAGCAT CGGACTCCAG AACTGTGATG ACACTGAAGT GGCCTCCTTG 
TGTTTGGAAG GCATCCGATG TGCAATCCGA ATCGCCTGCA TCTTTGGAAT GCAGCTGGAA 
CGAGATGCCT ATGTTCAGGC TCTTGCTCGC TTCTCCCTAC TCACAGCCAG CTCCAGCATC 
ACAGAAATGA AGCAGAAAAA CATCGACACC ATTAAGACGC TTATCACAGT GGCTCACACC 
GATGGCAACT ACCTTGGGAA TTCCTGGCAT GAGATCTTGA AATGCATCAG CCAGCTGGAG 
CTCGCTCAGC TGATAGGAAC CGGTGTGAAG ACGCGCTACC TGTCTGGATC TGGGCGTGAA 
AGAGAAGGGA GCCTGAAGGG CCACACATTG GCAGGAGAAG AGTTCATGGG CCTTGGCCTC 
GGTAATTTGG TGAGTGGCGG AGTGGATAAA AGACAGATGG CCAGCTTCCA AGAATCGGTT 
GGTGAGACCA GCTCGCAGAG TGTGGTTGTA GCTGTGGACA GGATTTTTAC TGGGTCTACC 
AGACTGGATG GAAATGCAAT AGTTGACTTT GTCCGCTGGC TGTGTGCTGT GTCCATGGAT 
GAACTGGCTT CCCCCCACCA TCCTCGCATG TTCAGCTTGC AGAAGATTGT GGAGATATCA 
TACTACAACA TGAATCGGAT CCGACTACAG TGGTCTCGAA TATGGCATGT GATTGGAGAT 
CACTTCAATA AGGTTGGCTG CAACCCTAAT GAAGATGTGG CTATCTTTGC TGTTGACTCA 
TTAAGGCAAC TCTCCATGAA GTTTCTTGAG AAGGGTGAAT TAGCCAACTT CCGTTTCCAG 
AAAGATTTTC TGAGGCCCTT TGAGCATATT ATGAAGAAAA ACAGGTCTCC CACCATCCGG 
GACATGGCGA TCCGCTGCAT TGCCCAGATG GTGAACTCCC AGGCGGCCAA CATCCGCTCA 
GGTTGGAAGA ACATCTTTGC CGTGTTCCAC CAGGCAGCCT CTGATCATGA TGGGAACATT 
GTGGAGCTGG CCTTCCAGAC CACTTGCCAC ATTGTCACAA CTATTTTCCA GCACCATTTT 
CCTGCAGCCA TCGATTCCTT TCAGGATGCT GTGAAGTGCT TATCAGAGTT CGCCTGCAAC 
GCCGCTTTCC CTGACACGAG CATGGAAGCG ATTCGGCTCA TCCGCTTCTG TGGCAAATAC 
GTCTCTGAGA GGCCTCGGGT GCTACAAGAA TACACAAGTG ATGACATGAA TGTAGCTCCT 
GGTGACAGAG TCTGGGTCCG AGGCTGGTTC CCCATCTTAT TCGAACTCTC CTGCATCATT 
AATAGATGCA AGTTAGATGT ACGAACAAGG GGACTCACAG TCATGTTTGA GATCATGAAG 
AGCTATGGCC ACACCTTTGA AAAGCACTGG TGGCAGGACC TGTTCAGAAT CGTGTTTCGG 
ATTTTTGACA ATATGAAACT CCCTGAGCAA CTGTCAGAGA AATCTGAGTG GATGACAACA 
ACCTGCAATC ACGCACTTTA TGCTATTTGT GATGTTTTTA CCCAGTTTTA TGAAGCTTTG 
AATGAAGTTC TTCTTTCTGA TGTATTTGCA CAATTGCAGT GGTGTGTCAA ACAAGATAAT 
GAACAGTTGG CGCGATCAGG TACAAATTGC TTAGAAAACT TAGTAATATC CAATGGAGAG 
AAATTCAGTC CTGAAGTCTG GGATGAAACC TGCAACTGTA TGTTGGATAT TTTCAAAACA 
ACCATCCCAC ATGTTTTGCT GACATGGAGA CCTGTAGGAA TGGAGGAAGA TTCATCAGAA 
AAGCATTTGG ATGTGGATCT GGACCGCCAG TCTTTAAGCA GCATAGATAA AAATCCCTCT 
GAGAGGGGAC AGAGCCAGCT CTCTAACCCA ACAGATGACA GCTGGAAGGG TAGACCATAC 
GCAAATCAGA AACTGTTTGC CAGCCTCCTC ATCAAGTGTG TGGTCCAGTT GGAATTGATA 
CAGACCATTG ACAACATTGT GTTCTACCCT GCGACGAGCA AAAAGGAGGA TGCAGAGCAC 
ATGGTTGCCG CCCAGCAAGA CACGCTGGAT GCAGATATCC ACATAGAGAC GGAGGATCAG 
GGCATGTATA AGTACATGTC TTCCCAGCAC CTCTTCAAGC TGTTGGACTG TTTGCAGGAA 
TCCCATTCAT TCTCAAAGGC CTTCAACTCC AATTACGAGC AGCGGACTGT CCTGTGGCGA 
GCAGGTTTTA AGGGCAAGTC TAAACCCAAT CTTCTAAAAC AAGAAACCAG CAGCCTGGCC 
TGTTGTTTGA GGATCCTGTT TCGAATGTAT GTTGATGAGA ACCGCAGGGA TTCCTGGGAA 
GAAATACAGC AGAGACTTTT AACTGTTTGC AGTGAAGCTC TTGCCTATTT CATCACTGTG 
AATTCTGAGA GCCATCGGGA GGCCTGGACA AGTCTCTTGT TGTTACTTCT AACTAAAACC 
CTCAAAATAA ATGATGAAAA GTTCAAAGCA CATGCTTCAA TGTACTACCC CTACTTGTGT 
GAAATTATGC AGTTTGACCT GATCCCTGAG CTCCGAGCAG TTCTGCGGAA GTTCTTCCTA 
CGGATAGGTG TTGTGTATAA GATATGGATA CCAGAAGAGC CATCACAGGT ACCAGCAGCA 
CTGTCACCAG TGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARFGEF2 ( XM_005260252.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005260309.1 Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 2 (ARFGEF2), transcript variant X1, mRNA.

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CloneID	OHu52862
Clone ID Related Accession (Same CDS sequence)	XM_005260252.3
Accession Version	XM_005260252.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260252.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAGA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCC 
GACAAGGAGG TGAAGCGGCC CCAGCACTCC CAGCTGCGCA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAAAT AGAAAAGCAG AGGCTTGGCA CTGCTGCACC ACCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTTCTT CCATTCGAGC TAGCTTGCCA GTCCAAGTCC 
CCAAGGGTAG TCAGCACATC CCTTGACTGC TTGCAGAAAC TCATCGCATA CGGGCACATC 
ACTGGCAACG CCCCTGACAG TGGAGCCCCT GGGAAGCGGC TGATCGACAG AATTGTTGAA 
ACCATTTGCA GTTGTTTTCA GGGCCCTCAG ACTGATGAAG GGGTTCAGTT ACAAATAATT 
AAGGCTCTTC TGACTGCAGT GACTTCCCCA CACATTGAAA TTCATGAGGG TACTATCCTG 
CAGACAGTGA GAACATGTTA CAATATCTAT TTGGCCAGCA AAAATCTCAT CAATCAAACC 
ACTGCCAAGG CTACCCTTAC TCAGATGCTG AACGTCATTT TCACCCGCAT GGAAAACCAA 
GTGTTGCAGG AGGCCAGAGA ACTGGAAAAA CCAATCCAGT CAAAACCCCA GTCCCCTGTG 
ATCCAAGCTG CAGCAGTATC CCCAAAGTTC GTTCGTTTGA AGCACAGTCA GGCACAAAGC 
AAACCAACAA CTCCCGAAAA AACAGATTTA ACCAACGGTG AACATGCCAG GAGTGATTCT 
GGAAAAGTAA GCACAGAAAA TGGAGACGCA CCCAGAGAAA GAGGCTCATC ACTGTCAGGG 
ACTGATGACG GAGCCCAGGA GGTGGTGAAG GACATCTTGG AAGATGTAGT CACATCTGCC 
ATTAAAGCAG CGGAAAAGCA TGGTCTGACA GAACCTGAGA GAGTTCTAGG TGAACTGGAG 
TGCCAGGAAT GTGCTATTCC CCCAGGAGTT GATGAAAACT CACAGACCAA CGGGATAGCC 
GATGACAGGC AGTCCTTGTC GTCAGCAGAT AATCTGGAAT CGGATGCACA AGGACATCAA 
GTGGCTGCCA GGTTCTCCCA CGTTCTGCAG AAGGATGCCT TCCTTGTGTT CCGCTCCCTG 
TGCAAGCTGT CCATGAAACC CCTTGGTGAA GGCCCTCCAG ACCCAAAATC CCATGAGCTG 
CGTTCCAAGG TGGTTTCCCT GCAGCTGCTC CTCTCTGTGT TGCAAAATGC TGGCCCCGTA 
TTCAGGACTC ACGAGATGTT CATCAATGCA ATCAAGCAAT ATCTCTGTGT GGCCTTGTCC 
AAAAACGGCG TCTCTTCAGT GCCTGATGTC TTTGAGCTCT CTCTTGCCAT TTTTCTTACT 
CTTCTTTCAA ACTTTAAAAT GCACTTGAAA ATGCAGATAG AGGTCTTTTT CAAAGAGATT 
TTCCTGAACA TTTTAGAAAC ATCAACAAGT TCTTTTGAGC ACAGGTGGAT GGTCATTCAG 
ACTCTGACGA GGATCTGTGC AGATGCCCAG TGTGTTGTGG ATATTTATGT CAACTACGAC 
TGTGATTTAA ATGCTGCTAA CATTTTTGAG CGCCTTGTAA ATGATTTATC CAAAATTGCT 
CAGGGAAGAA GTGGACATGA GCTGGGAATG ACACCTCTGC AGGAGCTCAG CCTGAGGAAG 
AAAGGCCTGG AGTGCCTCGT GTCCATTCTC AAGTGCATGG TGGAGTGGAG CAAAGACCTG 
TATGTGAATC CCAACCACCA GACCAGCCTC GGTCAGGAGA GGCTCACGGA TCAGGAAATA 
GGGGATGGGA AAGGCCTTGA CATGGCAAGA CGGTGTAGTG TGACGTCCAT GGAGTCCACA 
GTGTCCTCGG GGACCCAGAC AACTGTTCAG GATGACCCTG AGCAATTTGA GGTCATCAAG 
CAACAAAAAG AAATCATTGA ACACGGCATC GAGCTGTTCA ACAAGAAACC CAAGAGGGGG 
ATCCAGTTTC TCCAGGAGCA GGGCATGCTG GGAACGTCAG TTGAAGACAT AGCCCAATTC 
CTGCACCAGG AGGAGCGCCT GGATTCCACC CAAGTAGGCG ATTTTCTGGG AGATAGCGCA 
AGGTTCAACA AGGAGGTGAT GTATGCCTAC GTGGACCAAC TTGACTTCTG TGAAAAAGAA 
TTTGTCTCAG CCCTGCGGAC ATTCCTAGAA GGTTTCCGCC TACCTGGAGA AGCCCAAAAG 
ATTGACCGAT TAATGGAGAA GTTTGCCGCA AGATACATAG AATGCAACCA AGGGCAAACT 
CTGTTTGCTA GTGCTGACAC TGCTTATGTC CTAGCGTATT CAATTATTAT GCTGACTACA 
GACTTGCACA GTCCTCAGGT AAAAAATAAA ATGACGAAAG AGCAGTATAT TAAAATGAAT 
CGGGGTATCA ATGATAGTAA AGATCTGCCA GAAGAGTATC TCTCAAGCAT CTATGAAGAG 
ATAGAAGGCA AGAAAATTGC AATGAAAGAA ACAAAAGAGC TAACGATTGC AACCAAATCT 
ACTAAGCAGA ATGTAGCTAG TGAAAAGCAG CGGCGGCTGC TGTACAACTT AGAGATGGAG 
CAAATGGCTA AAACAGCCAA AGCTCTGATG GAGGCTGTGA GCCATGCCAA AGCCCCGTTT 
ACCAGTGCCA CTCACCTGGA CCATGTCCGG CCAATGTTCA AACTGGTGTG GACGCCACTA 
TTGGCAGCCT ACAGCATCGG ACTCCAGAAC TGTGATGACA CTGAAGTGGC CTCCTTGTGT 
TTGGAAGGCA TCCGATGTGC AATCCGAATC GCCTGCATCT TTGGAATGCA GCTGGAACGA 
GATGCCTATG TTCAGGCTCT TGCTCGCTTC TCCCTACTCA CAGCCAGCTC CAGCATCACA 
GAAATGAAGC AGAAAAACAT CGACACCATT AAGACGCTTA TCACAGTGGC TCACACCGAT 
GGCAACTACC TTGGGAATTC CTGGCATGAG ATCTTGAAAT GCATCAGCCA GCTGGAGCTC 
GCTCAGCTGA TAGGAACCGG TGTGAAGACG CGCTACCTGT CTGGATCTGG GCGTGAAAGA 
GAAGGGAGCC TGAAGGGCCA CACATTGGCA GGAGAAGAGT TCATGGGCCT TGGCCTCGGT 
AATTTGGTGA GTGGCGGAGT GGATAAAAGA CAGATGGCCA GCTTCCAAGA ATCGGTTGGT 
GAGACCAGCT CGCAGAGTGT GGTTGTAGCT GTGGACAGGA TTTTTACTGG GTCTACCAGA 
CTGGATGGAA ATGCAATAGT TGACTTTGTC CGCTGGCTGT GTGCTGTGTC CATGGATGAA 
CTGGCTTCCC CCCACCATCC TCGCATGTTC AGCTTGCAGA AGATTGTGGA GATATCATAC 
TACAACATGA ATCGGATCCG ACTACAGTGG TCTCGAATAT GGCATGTGAT TGGAGATCAC 
TTCAATAAGG TTGGCTGCAA CCCTAATGAA GATGTGGCTA TCTTTGCTGT TGACTCATTA 
AGGCAACTCT CCATGAAGTT TCTTGAGAAG GGTGAATTAG CCAACTTCCG TTTCCAGAAA 
GATTTTCTGA GGCCCTTTGA GCATATTATG AAGAAAAACA GGTCTCCCAC CATCCGGGAC 
ATGGCGATCC GCTGCATTGC CCAGATGGTG AACTCCCAGG CGGCCAACAT CCGCTCAGGT 
TGGAAGAACA TCTTTGCCGT GTTCCACCAG GCAGCCTCTG ATCATGATGG GAACATTGTG 
GAGCTGGCCT TCCAGACCAC TTGCCACATT GTCACAACTA TTTTCCAGCA CCATTTTCCT 
GCAGCCATCG ATTCCTTTCA GGATGCTGTG AAGTGCTTAT CAGAGTTCGC CTGCAACGCC 
GCTTTCCCTG ACACGAGCAT GGAAGCGATT CGGCTCATCC GCTTCTGTGG CAAATACGTC 
TCTGAGAGGC CTCGGGTGCT ACAAGAATAC ACAAGTGATG ACATGAATGT AGCTCCTGGT 
GACAGAGTCT GGGTCCGAGG CTGGTTCCCC ATCTTATTCG AACTCTCCTG CATCATTAAT 
AGATGCAAGT TAGATGTACG AACAAGGGGA CTCACAGTCA TGTTTGAGAT CATGAAGAGC 
TATGGCCACA CCTTTGAAAA GCACTGGTGG CAGGACCTGT TCAGAATCGT GTTTCGGATT 
TTTGACAATA TGAAACTCCC TGAGCAACTG TCAGAGAAAT CTGAGTGGAT GACAACAACC 
TGCAATCACG CACTTTATGC TATTTGTGAT GTTTTTACCC AGTTTTATGA AGCTTTGAAT 
GAAGTTCTTC TTTCTGATGT ATTTGCACAA TTGCAGTGGT GTGTCAAACA AGATAATGAA 
CAGTTGGCGC GATCAGGTAC AAATTGCTTA GAAAACTTAG TAATATCCAA TGGAGAGAAA 
TTCAGTCCTG AAGTCTGGGA TGAAACCTGC AACTGTATGT TGGATATTTT CAAAACAACC 
ATCCCACATG TTTTGCTGAC ATGGAGACCT GTAGGAATGG AGGAAGATTC ATCAGAAAAG 
CATTTGGATG TGGATCTGGA CCGCCAGTCT TTAAGCAGCA TAGATAAAAA TCCCTCTGAG 
AGGGGACAGA GCCAGCTCTC TAACCCAACA GATGACAGCT GGAAGGGTAG ACCATACGCA 
AATCAGAAAC TGTTTGCCAG CCTCCTCATC AAGTGTGTGG TCCAGTTGGA ATTGATACAG 
ACCATTGACA ACATTGTGTT CTACCCTGCG ACGAGCAAAA AGGAGGATGC AGAGCACATG 
GTTGCCGCCC AGCAAGACAC GCTGGATGCA GATATCCACA TAGAGACGGA GGATCAGGGC 
ATGTATAAGT ACATGTCTTC CCAGCACCTC TTCAAGCTGT TGGACTGTTT GCAGGAATCC 
CATTCATTCT CAAAGGCCTT CAACTCCAAT TACGAGCAGC GGACTGTCCT GTGGCGAGCA 
GGTTTTAAGG GCAAGTCTAA ACCCAATCTT CTAAAACAAG AAACCAGCAG CCTGGCCTGT 
TGTTTGAGGA TCCTGTTTCG AATGTATGTT GATGAGAACC GCAGGGATTC CTGGGAAGAA 
ATACAGCAGA GACTTTTAAC TGTTTGCAGT GAAGCTCTTG CCTATTTCAT CACTGTGAAT 
TCTGAGAGCC ATCGGGAGGC CTGGACAAGT CTCTTGTTGT TACTTCTAAC TAAAACCCTC 
AAAATAAATG ATGAAAAGTT CAAAGCACAT GCTTCAATGT ACTACCCCTA CTTGTGTGAA 
ATTATGCAGT TTGACCTGAT CCCTGAGCTC CGAGCAGTTC TGCGGAAGTT CTTCCTACGG 
ATAGGTGTTG TGTATAAGAT ATGGATACCA GAAGAGCCAT CACAGGTACC AGCAGCACTG 
TCACCAGTGT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005260309.1
CDS	170..5524
Translation	MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEIEKQRLGTAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICSCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPIQSKPQSPVIQAAAVSPKFVRLKHSQAQSKPTTPEKTDLTNGEHARSDSGKVSTENGDAPRERGSSLSGTDDGAQEVVKDILEDVVTSAIKAAEKHGLTEPERVLGELECQECAIPPGVDENSQTNGIADDRQSLSSADNLESDAQGHQVAARFSHVLQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRTHEMFINAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQTSLGQERLTDQEIGDGKGLDMARRCSVTSMESTVSSGTQTTVQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTSVEDIAQFLHQEERLDSTQVGDFLGDSARFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKELTIATKSTKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAIRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHTLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMAIRCIAQMVNSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTCHIVTTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWQDLFRIVFRIFDNMKLPEQLSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPEVWDETCNCMLDIFKTTIPHVLLTWRPVGMEEDSSEKHLDVDLDRQSLSSIDKNPSERGQSQLSNPTDDSWKGRPYANQKLFASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETEDQGMYKYMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWEEIQQRLLTVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKINDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGVVYKIWIPEEPSQVPAALSPVW

Target ORF information:

RefSeq Version	XM_005260252.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 2 (ARFGEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005260252.3

ORF Insert Sequence:

ATGCAGGAGA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCC 
GACAAGGAGG TGAAGCGGCC CCAGCACTCC CAGCTGCGCA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAAAT AGAAAAGCAG AGGCTTGGCA CTGCTGCACC ACCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTTCTT CCATTCGAGC TAGCTTGCCA GTCCAAGTCC 
CCAAGGGTAG TCAGCACATC CCTTGACTGC TTGCAGAAAC TCATCGCATA CGGGCACATC 
ACTGGCAACG CCCCTGACAG TGGAGCCCCT GGGAAGCGGC TGATCGACAG AATTGTTGAA 
ACCATTTGCA GTTGTTTTCA GGGCCCTCAG ACTGATGAAG GGGTTCAGTT ACAAATAATT 
AAGGCTCTTC TGACTGCAGT GACTTCCCCA CACATTGAAA TTCATGAGGG TACTATCCTG 
CAGACAGTGA GAACATGTTA CAATATCTAT TTGGCCAGCA AAAATCTCAT CAATCAAACC 
ACTGCCAAGG CTACCCTTAC TCAGATGCTG AACGTCATTT TCACCCGCAT GGAAAACCAA 
GTGTTGCAGG AGGCCAGAGA ACTGGAAAAA CCAATCCAGT CAAAACCCCA GTCCCCTGTG 
ATCCAAGCTG CAGCAGTATC CCCAAAGTTC GTTCGTTTGA AGCACAGTCA GGCACAAAGC 
AAACCAACAA CTCCCGAAAA AACAGATTTA ACCAACGGTG AACATGCCAG GAGTGATTCT 
GGAAAAGTAA GCACAGAAAA TGGAGACGCA CCCAGAGAAA GAGGCTCATC ACTGTCAGGG 
ACTGATGACG GAGCCCAGGA GGTGGTGAAG GACATCTTGG AAGATGTAGT CACATCTGCC 
ATTAAAGCAG CGGAAAAGCA TGGTCTGACA GAACCTGAGA GAGTTCTAGG TGAACTGGAG 
TGCCAGGAAT GTGCTATTCC CCCAGGAGTT GATGAAAACT CACAGACCAA CGGGATAGCC 
GATGACAGGC AGTCCTTGTC GTCAGCAGAT AATCTGGAAT CGGATGCACA AGGACATCAA 
GTGGCTGCCA GGTTCTCCCA CGTTCTGCAG AAGGATGCCT TCCTTGTGTT CCGCTCCCTG 
TGCAAGCTGT CCATGAAACC CCTTGGTGAA GGCCCTCCAG ACCCAAAATC CCATGAGCTG 
CGTTCCAAGG TGGTTTCCCT GCAGCTGCTC CTCTCTGTGT TGCAAAATGC TGGCCCCGTA 
TTCAGGACTC ACGAGATGTT CATCAATGCA ATCAAGCAAT ATCTCTGTGT GGCCTTGTCC 
AAAAACGGCG TCTCTTCAGT GCCTGATGTC TTTGAGCTCT CTCTTGCCAT TTTTCTTACT 
CTTCTTTCAA ACTTTAAAAT GCACTTGAAA ATGCAGATAG AGGTCTTTTT CAAAGAGATT 
TTCCTGAACA TTTTAGAAAC ATCAACAAGT TCTTTTGAGC ACAGGTGGAT GGTCATTCAG 
ACTCTGACGA GGATCTGTGC AGATGCCCAG TGTGTTGTGG ATATTTATGT CAACTACGAC 
TGTGATTTAA ATGCTGCTAA CATTTTTGAG CGCCTTGTAA ATGATTTATC CAAAATTGCT 
CAGGGAAGAA GTGGACATGA GCTGGGAATG ACACCTCTGC AGGAGCTCAG CCTGAGGAAG 
AAAGGCCTGG AGTGCCTCGT GTCCATTCTC AAGTGCATGG TGGAGTGGAG CAAAGACCTG 
TATGTGAATC CCAACCACCA GACCAGCCTC GGTCAGGAGA GGCTCACGGA TCAGGAAATA 
GGGGATGGGA AAGGCCTTGA CATGGCAAGA CGGTGTAGTG TGACGTCCAT GGAGTCCACA 
GTGTCCTCGG GGACCCAGAC AACTGTTCAG GATGACCCTG AGCAATTTGA GGTCATCAAG 
CAACAAAAAG AAATCATTGA ACACGGCATC GAGCTGTTCA ACAAGAAACC CAAGAGGGGG 
ATCCAGTTTC TCCAGGAGCA GGGCATGCTG GGAACGTCAG TTGAAGACAT AGCCCAATTC 
CTGCACCAGG AGGAGCGCCT GGATTCCACC CAAGTAGGCG ATTTTCTGGG AGATAGCGCA 
AGGTTCAACA AGGAGGTGAT GTATGCCTAC GTGGACCAAC TTGACTTCTG TGAAAAAGAA 
TTTGTCTCAG CCCTGCGGAC ATTCCTAGAA GGTTTCCGCC TACCTGGAGA AGCCCAAAAG 
ATTGACCGAT TAATGGAGAA GTTTGCCGCA AGATACATAG AATGCAACCA AGGGCAAACT 
CTGTTTGCTA GTGCTGACAC TGCTTATGTC CTAGCGTATT CAATTATTAT GCTGACTACA 
GACTTGCACA GTCCTCAGGT AAAAAATAAA ATGACGAAAG AGCAGTATAT TAAAATGAAT 
CGGGGTATCA ATGATAGTAA AGATCTGCCA GAAGAGTATC TCTCAAGCAT CTATGAAGAG 
ATAGAAGGCA AGAAAATTGC AATGAAAGAA ACAAAAGAGC TAACGATTGC AACCAAATCT 
ACTAAGCAGA ATGTAGCTAG TGAAAAGCAG CGGCGGCTGC TGTACAACTT AGAGATGGAG 
CAAATGGCTA AAACAGCCAA AGCTCTGATG GAGGCTGTGA GCCATGCCAA AGCCCCGTTT 
ACCAGTGCCA CTCACCTGGA CCATGTCCGG CCAATGTTCA AACTGGTGTG GACGCCACTA 
TTGGCAGCCT ACAGCATCGG ACTCCAGAAC TGTGATGACA CTGAAGTGGC CTCCTTGTGT 
TTGGAAGGCA TCCGATGTGC AATCCGAATC GCCTGCATCT TTGGAATGCA GCTGGAACGA 
GATGCCTATG TTCAGGCTCT TGCTCGCTTC TCCCTACTCA CAGCCAGCTC CAGCATCACA 
GAAATGAAGC AGAAAAACAT CGACACCATT AAGACGCTTA TCACAGTGGC TCACACCGAT 
GGCAACTACC TTGGGAATTC CTGGCATGAG ATCTTGAAAT GCATCAGCCA GCTGGAGCTC 
GCTCAGCTGA TAGGAACCGG TGTGAAGACG CGCTACCTGT CTGGATCTGG GCGTGAAAGA 
GAAGGGAGCC TGAAGGGCCA CACATTGGCA GGAGAAGAGT TCATGGGCCT TGGCCTCGGT 
AATTTGGTGA GTGGCGGAGT GGATAAAAGA CAGATGGCCA GCTTCCAAGA ATCGGTTGGT 
GAGACCAGCT CGCAGAGTGT GGTTGTAGCT GTGGACAGGA TTTTTACTGG GTCTACCAGA 
CTGGATGGAA ATGCAATAGT TGACTTTGTC CGCTGGCTGT GTGCTGTGTC CATGGATGAA 
CTGGCTTCCC CCCACCATCC TCGCATGTTC AGCTTGCAGA AGATTGTGGA GATATCATAC 
TACAACATGA ATCGGATCCG ACTACAGTGG TCTCGAATAT GGCATGTGAT TGGAGATCAC 
TTCAATAAGG TTGGCTGCAA CCCTAATGAA GATGTGGCTA TCTTTGCTGT TGACTCATTA 
AGGCAACTCT CCATGAAGTT TCTTGAGAAG GGTGAATTAG CCAACTTCCG TTTCCAGAAA 
GATTTTCTGA GGCCCTTTGA GCATATTATG AAGAAAAACA GGTCTCCCAC CATCCGGGAC 
ATGGCGATCC GCTGCATTGC CCAGATGGTG AACTCCCAGG CGGCCAACAT CCGCTCAGGT 
TGGAAGAACA TCTTTGCCGT GTTCCACCAG GCAGCCTCTG ATCATGATGG GAACATTGTG 
GAGCTGGCCT TCCAGACCAC TTGCCACATT GTCACAACTA TTTTCCAGCA CCATTTTCCT 
GCAGCCATCG ATTCCTTTCA GGATGCTGTG AAGTGCTTAT CAGAGTTCGC CTGCAACGCC 
GCTTTCCCTG ACACGAGCAT GGAAGCGATT CGGCTCATCC GCTTCTGTGG CAAATACGTC 
TCTGAGAGGC CTCGGGTGCT ACAAGAATAC ACAAGTGATG ACATGAATGT AGCTCCTGGT 
GACAGAGTCT GGGTCCGAGG CTGGTTCCCC ATCTTATTCG AACTCTCCTG CATCATTAAT 
AGATGCAAGT TAGATGTACG AACAAGGGGA CTCACAGTCA TGTTTGAGAT CATGAAGAGC 
TATGGCCACA CCTTTGAAAA GCACTGGTGG CAGGACCTGT TCAGAATCGT GTTTCGGATT 
TTTGACAATA TGAAACTCCC TGAGCAACTG TCAGAGAAAT CTGAGTGGAT GACAACAACC 
TGCAATCACG CACTTTATGC TATTTGTGAT GTTTTTACCC AGTTTTATGA AGCTTTGAAT 
GAAGTTCTTC TTTCTGATGT ATTTGCACAA TTGCAGTGGT GTGTCAAACA AGATAATGAA 
CAGTTGGCGC GATCAGGTAC AAATTGCTTA GAAAACTTAG TAATATCCAA TGGAGAGAAA 
TTCAGTCCTG AAGTCTGGGA TGAAACCTGC AACTGTATGT TGGATATTTT CAAAACAACC 
ATCCCACATG TTTTGCTGAC ATGGAGACCT GTAGGAATGG AGGAAGATTC ATCAGAAAAG 
CATTTGGATG TGGATCTGGA CCGCCAGTCT TTAAGCAGCA TAGATAAAAA TCCCTCTGAG 
AGGGGACAGA GCCAGCTCTC TAACCCAACA GATGACAGCT GGAAGGGTAG ACCATACGCA 
AATCAGAAAC TGTTTGCCAG CCTCCTCATC AAGTGTGTGG TCCAGTTGGA ATTGATACAG 
ACCATTGACA ACATTGTGTT CTACCCTGCG ACGAGCAAAA AGGAGGATGC AGAGCACATG 
GTTGCCGCCC AGCAAGACAC GCTGGATGCA GATATCCACA TAGAGACGGA GGATCAGGGC 
ATGTATAAGT ACATGTCTTC CCAGCACCTC TTCAAGCTGT TGGACTGTTT GCAGGAATCC 
CATTCATTCT CAAAGGCCTT CAACTCCAAT TACGAGCAGC GGACTGTCCT GTGGCGAGCA 
GGTTTTAAGG GCAAGTCTAA ACCCAATCTT CTAAAACAAG AAACCAGCAG CCTGGCCTGT 
TGTTTGAGGA TCCTGTTTCG AATGTATGTT GATGAGAACC GCAGGGATTC CTGGGAAGAA 
ATACAGCAGA GACTTTTAAC TGTTTGCAGT GAAGCTCTTG CCTATTTCAT CACTGTGAAT 
TCTGAGAGCC ATCGGGAGGC CTGGACAAGT CTCTTGTTGT TACTTCTAAC TAAAACCCTC 
AAAATAAATG ATGAAAAGTT CAAAGCACAT GCTTCAATGT ACTACCCCTA CTTGTGTGAA 
ATTATGCAGT TTGACCTGAT CCCTGAGCTC CGAGCAGTTC TGCGGAAGTT CTTCCTACGG 
ATAGGTGTTG TGTATAAGAT ATGGATACCA GAAGAGCCAT CACAGGTACC AGCAGCACTG 
TCACCAGTGT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Enhancement of ?-catenin activity by BIG1 plus BIG2 via Arf activation and cAMP signals.
Proceedings of the National Academy of Sciences of the United States of America113(21)5946-51(2016 May)
Li CC,Le K,Kato J,Moss J,Vaughan M

Genome-wide association study of proneness to anger.
PloS one9(1)e87257(2014)
Mick E,McGough J,Deutsch CK,Frazier JA,Kennedy D,Goldberg RJ

West syndrome, microcephaly, grey matter heterotopia and hypoplasia of corpus callosum due to a novel ARFGEF2 mutation.
Journal of medical genetics50(11)772-5(2013 Nov)
Banne E,Atawneh O,Henneke M,Brockmann K,G?rtner J,Elpeleg O,Edvardson S

Elaborating the phenotypic spectrum associated with mutations in ARFGEF2: case study and literature review.
European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society17(6)666-70(2013 Nov)
Tanyal?in I,Verhelst H,Halley DJ,Vanderhasselt T,Villard L,Goizet C,Lissens W,Mancini GM,Jansen AC

Arf guanine nucleotide-exchange factors BIG1 and BIG2 regulate nonmuscle myosin IIA activity by anchoring myosin phosphatase complex.
Proceedings of the National Academy of Sciences of the United States of America110(34)E3162-70(2013 Aug)
Le K,Li CC,Ye G,Moss J,Vaughan M

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ARFGEF2 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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