arfgef2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	arfgef2
Entrez Gene ID	100334572
Full Name	ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
Synonyms	si:dkey-192i18.4
General protein information	Preferred Names ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) Names brefeldin A-inhibited guanine nucleotide-exchange protein 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002666467.4	XP_002666513.2	brefeldin A-inhibited guanine nucleotide-exchange protein 2
XM_002666467.5	XP_002666513.2	brefeldin A-inhibited guanine nucleotide-exchange protein 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ARFGEF2	XP_534448.1
Arabidopsis thaliana (thale cress)	AT4G38200	NP_195533.2
Pan troglodytes (chimpanzee)	ARFGEF2	XP_001165517.2
	ARFGEF2	XP_002798173.1
Schizosaccharomyces pombe (fission yeast)	sec71	NP_594954.1
Arabidopsis thaliana (thale cress)	AT4G35380	NP_195264.2
Homo sapiens (human)	ARFGEF2	NP_006411.2
Rattus norvegicus (Norway rat)	Arfgef2	NP_851597.2
Danio rerio (zebrafish)	arfgef2	XP_002666513.2
Gallus gallus (chicken)	ARFGEF2	XP_417388.3
Xenopus tropicalis (tropical clawed frog)	arfgef2	XP_002933226.2
Bos taurus (cattle)	ARFGEF2	NP_001192581.1
Mus musculus (house mouse)	Arfgef2	NP_001078964.1

Gene Ontology

The following arfgef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arfgef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa27634	XM_002666467.5 Latest version!	Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (arfgef2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
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ODa27634	XM_002666467.4	Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (arfgef2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arfgef2 ( XM_002666467.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002666513.2 Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (arfgef2), mRNA.

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CloneID	ODa27634
Clone ID Related Accession (Same CDS sequence)	XM_002666467.4 , XM_002666467.5
Accession Version	XM_002666467.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002666467.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAC AGGAAGCGCT GGCAACACAT CGACGGCATA CGCAGACAGA AAGCCTGAGC 
ACCAAGAGCA TGTTTCTGTC TCGGGCGCTG GAGAAAATCC TCGCGGATAA AGAGGTGAAG 
CGGAGCCAGC ACAACCAGCT GCGCAAGGCT TGTCAAGTGG CGCTAGATGA AATCAAGCAT 
GAACTGGAAA AGCAGAAGGA CGGCACTGTT GTTCCTCCCA GAGCCAACTA CATTGAGGCT 
GACAAATATG TCCTGCCTTT TGAGCTGGCA TGTCAATCCA AGTCTCCACG TATCGTCAGC 
ACATCTCTCG ATTGCCTACA GAAACTCATT GCGTATGGGC ACATCACAGG AAACGCCCCT 
GACAGCGCAG CACCAGGGAA GCGACTGATT GACCGTCTGG TGGAGACCAT CTGTAACTGC 
TTTCAGGGAC CCCAGACTGA TGAGGGCGTG CAGCTTCAGA TCATTAAGGC TCTGCTGACT 
GCTGTGACAT CACCACACAT AGAGATCCAT GAGGGGACGA TTCTGCTAAC TGTGCGCACC 
TGCTACAACA TCTACCTGGC CAGCCGTAAT CTGATCAACC AGACCACAGC CAAGGCCACT 
CTCACACAGA TGCTCAATGT CATCTTTACC CGCATGGAGA ACCAGGCGGC TTTAGAAGCT 
CAGGAAGCAG AGAAGGAGCG GCAGCGTTTA CAGCTGCGCA ATCCTTCCCC AGTATCCAGT 
AACCCTTCAC CTGTCAACCA AAATCCTTCT CAAACTCCTG GACGGGGATC GGGATCCCCA 
TGGATGGGAC GAGCCTGCTC TGAACAGAAC GGTTCGCCTT GTCTCAGCCC TCCTGGGGTC 
CCCTCTACTC CCTCCACCCC TCAAGACTCA TCCTCCATCA ACAGCCGGCC AGAGGAGGAG 
TTTGCCACGG ATAAACAGAG CACAAAGGAC ATTACACATC CAGATGAGGA GCTTCAGGAG 
TCAGACCAAA CGACTGTCGA TCCTTCTGTG ACGGGAGGTG TTGAAGGAGA GGAAACGCAT 
CCGCAGTCTG AAACAGAAGC AGGTTCTGAG GTCCTGGAGG AGACAGAGCC GGACAGTGGA 
TTGGGAGACA GCTCTGTGGA AGGAGGACTA GATCAACATT CTGACTCAGA AACCAGAATG 
CCGCCCCCTG TTGTCCGAGT TGACACACAG CAGATGAATG GAATGGTGGA TGACCGCGGC 
TCAGAGTCCT CCACAGACAT GCTGGATGCA GAAAGCATTC ATGGCAGTCA AACAGCTGCG 
CGCTTCTCCC ACATTCTCCA GAAAGATGCT TTCCTTGTGT TTCGCTCGCT CTGCAAGCTC 
TCCATGAAGC CTCTGGCTGA TGGACCACCA GACCCAAAGT CTCATGAGTT GCGCTCTAAG 
GTTGTTTCTC TGCAGCTGTT GCTGTCGGTG CTGCAGGGCG CTGGACCTGT GTTTCGAACC 
CATGAGATGT TCGTGAACGC CATCAAACAG TACCTGTGTG TGGCGCTCTC TAAAAACGGG 
GTCTCCTCTG TGCCTGAAGT ATTTGAACTG TCTCTTGCCA TCTTCCTCAC CTTGCTCTCC 
CACTTCAAAG TGCACCTTAA GATGCAGATC GAGGTGTTCT TCAGGGAGAT CTTTCTGACA 
ATCCTGGAGA CATCGTCCAG CTCTTTTGAG CACAAATGGA TGGTCATTCA GACTCTAACA 
CGAATATGTG CAGACGCACA GTGTGTTGTG GATATTTATG TTAACTATGA TTGTGATCTC 
AACGCTGCAA ACATTTTTGA GCGGCTGGTA AATGACCTGT CTAAAATCGC CCAGGGGAGA 
AGTGGACAGG AGCTGGGCAT GACTCCATTA CAGGAGCTGA GTTTGAGAAA GAAAGGCCTG 
GAGTGTCTGG TGTCTATTTT GAAGTGCATG GTGGAATGGA GCAGAGACAT GTACGTCAAC 
CCCAACCTGC AGGCTAACCT TGGTCAGGAA CGTCCAGCAG AGGGCGACAG TGCTGACGTG 
AAGCTGTCTG AGCATCTCTC CTCTCGCAGA GATAGCGTGA GCTCTCTGGA CTCCACCGTG 
TCGTCTGGAG TTCAGCAATC TCAGCCTGAT CACCCCGAGC AGTATGAGGT CATCAAGCAG 
CAGAAGGAGA TCATCGAGCA TGGCATTGAG CTGTTTAACA AGAAACCAAA AAGGGGATTG 
CAATATCTGC AGGAGCAAGG CATGCTGGGA ACTTCACCAG AGGACATTGC ACAGTTCCTT 
CAACAGGAGG AGCGATTAGA CACGACTCAG GTTGGCGAGT TCCTGGGTGA AAATGTGAAG 
TTTAATAAGG AGGTTATGTA CTGCTACGTG GACCGACTGG ACTTCTGTGG CAAAGACTTT 
GTGTCCGCAC TTCGTGCCTT TTTGGAGGGC TTCAGGCTTC CAGGTGAAGC CCAGAAAATC 
GATCGGCTGA TGGAGAAGTT TGCCGCCCGC TACCTGGAGT GCAATCAGGG GCAGACTCTG 
TTTGCCAGCG CCGACACTGC TTACGTTCTG GCCTACTCCA TCATCATGTT AACCACTGAC 
CTGCACAGTC CACAGGTGAA GAACAAAATG ACGAAGGAGC AGTACATAAA AATGAACCGT 
GGCATTAATG ACAGTAAAGA CCTGCCTGAG GAGTATCTGT CCTCCATCTA CGATGAAATA 
GCAGGGAAGA AGATCGCCAT GAAGGAGAGC AAGGAGTACT CCATCACTCC CAAATCCAGC 
AAGCAGAATG TAGCCAATGA GAAGCAGAGA AGGCTGCTGT ATAACATGGA GATGGAGCAG 
ATGGCTAAAA CCGCTAAAGC TCTGATGGAG GCAGTCAGTC ACGCTCAGGC CCCCTTCTTC 
AGTGCCACTC ACCTAGAGCA CGTCCGGCCC ATGTTCAAGC TGGCCTGGAC TCCCTTGTTG 
GCTGCGTTCA GCGTCGGCCT TCAGGATTGT GACGATCAGG ATGTGGCATC TCTGTGCCTG 
GAGGGGATTC GCTGTGCTAT CAGGATCGCC TGCATCTTTA ATATGCAGTT GGAAAGAGAT 
GCTTATATCC AGGCCTTGGC CCGGTTTACA CTGCTCACTG CCAGCTCAAG TATCACAGAG 
ATGAAGCAGA AGAACATTGA CACCATAAAG ACTCTTATCA TGGTCGCCCA CACTGACGGA 
AACTACCTGG GAAACTCCTG GCATGAGATT CTACGGTGTA TCAGTCAGCT GGAGCTGGCT 
CAGTTGATTG GCACAGGGGT GAAGATGCGT ATCTCCGGTG TGGTCCGGGA CCAGGGAGGC 
GGCATAAAGG GCTTCCCATC AGGCGGAGAG GAATTCATGC CCCTCGGCTT AGGTACTCTG 
GTTGGAGGTC CAGACAAGCG TCAGATGGCT CATATCCAGG AGTCAGTAGG GGAGACCAGC 
TCTCAGAGTG TTGTGGTGGC TGTGGACAGA ATCTTCACTG GATCCACCAG GCTTGACGGA 
AATGCAATCG TGGACTTTGT GCGATGGCTG TGTGCCGTCT CAATGGACGA ACTGGCCTCT 
GCTCACCAGC CCCGCATGTT CAGTCTGCAG AAGATCGTGG AGATCTCGTA CTACAACATG 
AACCGCATCC GCCTGCAGTG GTCACGCATT TGGCAGGTCA TCGGAGATCA TTTCAACAAG 
GTTGGTTGTA ACCCGAATGA AGATGTGGCC ATCTTTGCTG TGGACTCCCT GCGACAACTT 
TCTATGAAGT TTTTAGAGAA AGGAGAACTG GCCAATTTCC GCTTCCAAAA GGACTTTTTA 
AGGCCATTCG AGCACATCGT GAAGAAGAAC AGGTCGCCCA CTATCCGGGA CATGGTGATA 
CGTTGTGTGG CCCAGATGGT CAACTCTCAG GCTGCTAATA TTCGCTCTGG CTGGAAAAAC 
ATCTTCTCTG TGTTTCATCA AGCAGCGTCA GATCATGATG AGAACATTGT GGATCTTGCA 
TTTCAGACCA CAGGGCACAT TGTTATGAAC ACATTTCAGC AGCATTTTGC AGCTGCCATT 
GACTCCTTCC AGGATGCAGT GAAGTGTTTG TCGGAGTTTG TTTGTAACGC TGCGTTTCCT 
GACACCAGTA TGGAAGCCAT TCGGCTAATT CGCCACTGTG CTAAATATGT GTCTGAAAGG 
CCGCAGGCCC TGAGGGAGTA CACTAGTGAT GACATGAACG TGGCCCCTGG TGACCGGGTT 
TGGGTGCGAG GCTGGTTCCC CATCCTGTTT GAGCTTTCCT GCATCATCAA TCGCTGCAAA 
CTTGATGTCA GGACCAGGGG TCTGACCGTC ATGTTTGAGA TCATGAAGAG TTACGGACAC 
ACCTTTGAAA AGCACTGGTG GCATGATCTC TTTCGGATCA TCTTCAGAAT ATTCGACAAC 
ATGAAGCTCC CTGAGCAGCA GACTGAAAAA ACAGAGTGGA TGACGACGAC ATGTAACCAC 
GCTCTCTACG CCATCTGCGA TGTGTTCACG CAGTTCTACG AGCCTCTCAG TGAAGTGCTA 
CTGGCGGACA TTTTTGCGCA GCTGCAGTGG TGCGTCAAAC AAGATAATGA GCAGCTGGCA 
CGCTCGGGCA CGAACTGTCT GGAGAACCTG GTGATCCTGA ACGGGGAGAA GTTCAGTCCG 
GAGGTGTGGG ACGTCACCTG CTCCTGCATG CTGGACATCT TCCAGACCAC CAGCCCTCAT 
GCCTTGCTGA CATGGAGACC AGCTGGACAG GAAGAGGAGG TGGGAGAAGG GAAACACATG 
GATGTTGAGG TGGATTCCCA GTCACAGAGC AGCTTTGAGA GAACTCTGTC AGAGAGAGGC 
CACAGTCAAA TGTCCACCGC CAGCGATGAA ACATGGAAAG GAAAGTCCCA CACCAGGGTT 
TCGGACCACA GGCTGTTTGC AGGGCTGCTT ATAAAGTGTG TCGTACAGCT GGAGTTGATT 
CAGACAATAG ACAACATTGT ATTCTACCCT GCTACCAGCA AGAGAGAAGA TGCTGAGAAC 
ATGGCCGCTG CTCAGCGAGA TGCACTTGAG CAGGCAGAAG AGGGAGAGGC GGAGGTGGAT 
CAGGGCATGT ACAGACACCT GTCATCCCAG CACCTCTTCA AACTGCTCGA CTGCCTCCTC 
GAGTCCCACA GGTTTGCCAA GGACTTCAAC TCCAACAATG AGCAGCGCAC GGCCCTCTGG 
AGAGCAGGCT TTAAGGGGAA GTCAAAACCG AATCTTCTGA AGCAGGAGAC CAGCAGTCTG 
GCCTGCAGTC TAAGGATCCT CTTTCGCATG TACTCCGACA CGCAGCTGCG AGACTCCTGG 
CCAGACATCC AGACACGTCT GCTGCATGTG TGCAGTGAAG CCCTGGCGTA CTTCATCAGT 
CTGACCTCAG AGAGTCATCG TGAAGCGTGG ACGAGTCTCC TGCTGCTGCT GCTCACCCGC 
ACACTCCGAC TGCCTGATGA TAAATTCAAG CCTCATGCCT CCTGCTACTA CCACCATCTA 
TGTGAGATGA TGCAGTTCGA CCTCATTCCG GAGCTCCGCG CTGTTCTGCG GCGATTTTTC 
CTCCGCATCG GTTCCGTCTT CCACATCGCT GCTCCTGAAC TGGGCCAAGC ACAGCCTTCG 
TCATCATCAT CTTCATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002666513.2
CDS	293..5833
Translation	MKEQEALATHRRHTQTESLSTKSMFLSRALEKILADKEVKRSQHNQLRKACQVALDEIKHELEKQKDGTVVPPRANYIEADKYVLPFELACQSKSPRIVSTSLDCLQKLIAYGHITGNAPDSAAPGKRLIDRLVETICNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILLTVRTCYNIYLASRNLINQTTAKATLTQMLNVIFTRMENQAALEAQEAEKERQRLQLRNPSPVSSNPSPVNQNPSQTPGRGSGSPWMGRACSEQNGSPCLSPPGVPSTPSTPQDSSSINSRPEEEFATDKQSTKDITHPDEELQESDQTTVDPSVTGGVEGEETHPQSETEAGSEVLEETEPDSGLGDSSVEGGLDQHSDSETRMPPPVVRVDTQQMNGMVDDRGSESSTDMLDAESIHGSQTAARFSHILQKDAFLVFRSLCKLSMKPLADGPPDPKSHELRSKVVSLQLLLSVLQGAGPVFRTHEMFVNAIKQYLCVALSKNGVSSVPEVFELSLAIFLTLLSHFKVHLKMQIEVFFREIFLTILETSSSSFEHKWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGQELGMTPLQELSLRKKGLECLVSILKCMVEWSRDMYVNPNLQANLGQERPAEGDSADVKLSEHLSSRRDSVSSLDSTVSSGVQQSQPDHPEQYEVIKQQKEIIEHGIELFNKKPKRGLQYLQEQGMLGTSPEDIAQFLQQEERLDTTQVGEFLGENVKFNKEVMYCYVDRLDFCGKDFVSALRAFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYDEIAGKKIAMKESKEYSITPKSSKQNVANEKQRRLLYNMEMEQMAKTAKALMEAVSHAQAPFFSATHLEHVRPMFKLAWTPLLAAFSVGLQDCDDQDVASLCLEGIRCAIRIACIFNMQLERDAYIQALARFTLLTASSSITEMKQKNIDTIKTLIMVAHTDGNYLGNSWHEILRCISQLELAQLIGTGVKMRISGVVRDQGGGIKGFPSGGEEFMPLGLGTLVGGPDKRQMAHIQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASAHQPRMFSLQKIVEISYYNMNRIRLQWSRIWQVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIVKKNRSPTIRDMVIRCVAQMVNSQAANIRSGWKNIFSVFHQAASDHDENIVDLAFQTTGHIVMNTFQQHFAAAIDSFQDAVKCLSEFVCNAAFPDTSMEAIRLIRHCAKYVSERPQALREYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWHDLFRIIFRIFDNMKLPEQQTEKTEWMTTTCNHALYAICDVFTQFYEPLSEVLLADIFAQLQWCVKQDNEQLARSGTNCLENLVILNGEKFSPEVWDVTCSCMLDIFQTTSPHALLTWRPAGQEEEVGEGKHMDVEVDSQSQSSFERTLSERGHSQMSTASDETWKGKSHTRVSDHRLFAGLLIKCVVQLELIQTIDNIVFYPATSKREDAENMAAAQRDALEQAEEGEAEVDQGMYRHLSSQHLFKLLDCLLESHRFAKDFNSNNEQRTALWRAGFKGKSKPNLLKQETSSLACSLRILFRMYSDTQLRDSWPDIQTRLLHVCSEALAYFISLTSESHREAWTSLLLLLLTRTLRLPDDKFKPHASCYYHHLCEMMQFDLIPELRAVLRRFFLRIGSVFHIAAPELGQAQPSSSSSSS

Target ORF information:

RefSeq Version	XM_002666467.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (arfgef2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002666467.4

ORF Insert Sequence:

ATGAAAGAAC AGGAAGCGCT GGCAACACAT CGACGGCATA CGCAGACAGA AAGCCTGAGC 
ACCAAGAGCA TGTTTCTGTC TCGGGCGCTG GAGAAAATCC TCGCGGATAA AGAGGTGAAG 
CGGAGCCAGC ACAACCAGCT GCGCAAGGCT TGTCAAGTGG CGCTAGATGA AATCAAGCAT 
GAACTGGAAA AGCAGAAGGA CGGCACTGTT GTTCCTCCCA GAGCCAACTA CATTGAGGCT 
GACAAATATG TCCTGCCTTT TGAGCTGGCA TGTCAATCCA AGTCTCCACG TATCGTCAGC 
ACATCTCTCG ATTGCCTACA GAAACTCATT GCGTATGGGC ACATCACAGG AAACGCCCCT 
GACAGCGCAG CACCAGGGAA GCGACTGATT GACCGTCTGG TGGAGACCAT CTGTAACTGC 
TTTCAGGGAC CCCAGACTGA TGAGGGCGTG CAGCTTCAGA TCATTAAGGC TCTGCTGACT 
GCTGTGACAT CACCACACAT AGAGATCCAT GAGGGGACGA TTCTGCTAAC TGTGCGCACC 
TGCTACAACA TCTACCTGGC CAGCCGTAAT CTGATCAACC AGACCACAGC CAAGGCCACT 
CTCACACAGA TGCTCAATGT CATCTTTACC CGCATGGAGA ACCAGGCGGC TTTAGAAGCT 
CAGGAAGCAG AGAAGGAGCG GCAGCGTTTA CAGCTGCGCA ATCCTTCCCC AGTATCCAGT 
AACCCTTCAC CTGTCAACCA AAATCCTTCT CAAACTCCTG GACGGGGATC GGGATCCCCA 
TGGATGGGAC GAGCCTGCTC TGAACAGAAC GGTTCGCCTT GTCTCAGCCC TCCTGGGGTC 
CCCTCTACTC CCTCCACCCC TCAAGACTCA TCCTCCATCA ACAGCCGGCC AGAGGAGGAG 
TTTGCCACGG ATAAACAGAG CACAAAGGAC ATTACACATC CAGATGAGGA GCTTCAGGAG 
TCAGACCAAA CGACTGTCGA TCCTTCTGTG ACGGGAGGTG TTGAAGGAGA GGAAACGCAT 
CCGCAGTCTG AAACAGAAGC AGGTTCTGAG GTCCTGGAGG AGACAGAGCC GGACAGTGGA 
TTGGGAGACA GCTCTGTGGA AGGAGGACTA GATCAACATT CTGACTCAGA AACCAGAATG 
CCGCCCCCTG TTGTCCGAGT TGACACACAG CAGATGAATG GAATGGTGGA TGACCGCGGC 
TCAGAGTCCT CCACAGACAT GCTGGATGCA GAAAGCATTC ATGGCAGTCA AACAGCTGCG 
CGCTTCTCCC ACATTCTCCA GAAAGATGCT TTCCTTGTGT TTCGCTCGCT CTGCAAGCTC 
TCCATGAAGC CTCTGGCTGA TGGACCACCA GACCCAAAGT CTCATGAGTT GCGCTCTAAG 
GTTGTTTCTC TGCAGCTGTT GCTGTCGGTG CTGCAGGGCG CTGGACCTGT GTTTCGAACC 
CATGAGATGT TCGTGAACGC CATCAAACAG TACCTGTGTG TGGCGCTCTC TAAAAACGGG 
GTCTCCTCTG TGCCTGAAGT ATTTGAACTG TCTCTTGCCA TCTTCCTCAC CTTGCTCTCC 
CACTTCAAAG TGCACCTTAA GATGCAGATC GAGGTGTTCT TCAGGGAGAT CTTTCTGACA 
ATCCTGGAGA CATCGTCCAG CTCTTTTGAG CACAAATGGA TGGTCATTCA GACTCTAACA 
CGAATATGTG CAGACGCACA GTGTGTTGTG GATATTTATG TTAACTATGA TTGTGATCTC 
AACGCTGCAA ACATTTTTGA GCGGCTGGTA AATGACCTGT CTAAAATCGC CCAGGGGAGA 
AGTGGACAGG AGCTGGGCAT GACTCCATTA CAGGAGCTGA GTTTGAGAAA GAAAGGCCTG 
GAGTGTCTGG TGTCTATTTT GAAGTGCATG GTGGAATGGA GCAGAGACAT GTACGTCAAC 
CCCAACCTGC AGGCTAACCT TGGTCAGGAA CGTCCAGCAG AGGGCGACAG TGCTGACGTG 
AAGCTGTCTG AGCATCTCTC CTCTCGCAGA GATAGCGTGA GCTCTCTGGA CTCCACCGTG 
TCGTCTGGAG TTCAGCAATC TCAGCCTGAT CACCCCGAGC AGTATGAGGT CATCAAGCAG 
CAGAAGGAGA TCATCGAGCA TGGCATTGAG CTGTTTAACA AGAAACCAAA AAGGGGATTG 
CAATATCTGC AGGAGCAAGG CATGCTGGGA ACTTCACCAG AGGACATTGC ACAGTTCCTT 
CAACAGGAGG AGCGATTAGA CACGACTCAG GTTGGCGAGT TCCTGGGTGA AAATGTGAAG 
TTTAATAAGG AGGTTATGTA CTGCTACGTG GACCGACTGG ACTTCTGTGG CAAAGACTTT 
GTGTCCGCAC TTCGTGCCTT TTTGGAGGGC TTCAGGCTTC CAGGTGAAGC CCAGAAAATC 
GATCGGCTGA TGGAGAAGTT TGCCGCCCGC TACCTGGAGT GCAATCAGGG GCAGACTCTG 
TTTGCCAGCG CCGACACTGC TTACGTTCTG GCCTACTCCA TCATCATGTT AACCACTGAC 
CTGCACAGTC CACAGGTGAA GAACAAAATG ACGAAGGAGC AGTACATAAA AATGAACCGT 
GGCATTAATG ACAGTAAAGA CCTGCCTGAG GAGTATCTGT CCTCCATCTA CGATGAAATA 
GCAGGGAAGA AGATCGCCAT GAAGGAGAGC AAGGAGTACT CCATCACTCC CAAATCCAGC 
AAGCAGAATG TAGCCAATGA GAAGCAGAGA AGGCTGCTGT ATAACATGGA GATGGAGCAG 
ATGGCTAAAA CCGCTAAAGC TCTGATGGAG GCAGTCAGTC ACGCTCAGGC CCCCTTCTTC 
AGTGCCACTC ACCTAGAGCA CGTCCGGCCC ATGTTCAAGC TGGCCTGGAC TCCCTTGTTG 
GCTGCGTTCA GCGTCGGCCT TCAGGATTGT GACGATCAGG ATGTGGCATC TCTGTGCCTG 
GAGGGGATTC GCTGTGCTAT CAGGATCGCC TGCATCTTTA ATATGCAGTT GGAAAGAGAT 
GCTTATATCC AGGCCTTGGC CCGGTTTACA CTGCTCACTG CCAGCTCAAG TATCACAGAG 
ATGAAGCAGA AGAACATTGA CACCATAAAG ACTCTTATCA TGGTCGCCCA CACTGACGGA 
AACTACCTGG GAAACTCCTG GCATGAGATT CTACGGTGTA TCAGTCAGCT GGAGCTGGCT 
CAGTTGATTG GCACAGGGGT GAAGATGCGT ATCTCCGGTG TGGTCCGGGA CCAGGGAGGC 
GGCATAAAGG GCTTCCCATC AGGCGGAGAG GAATTCATGC CCCTCGGCTT AGGTACTCTG 
GTTGGAGGTC CAGACAAGCG TCAGATGGCT CATATCCAGG AGTCAGTAGG GGAGACCAGC 
TCTCAGAGTG TTGTGGTGGC TGTGGACAGA ATCTTCACTG GATCCACCAG GCTTGACGGA 
AATGCAATCG TGGACTTTGT GCGATGGCTG TGTGCCGTCT CAATGGACGA ACTGGCCTCT 
GCTCACCAGC CCCGCATGTT CAGTCTGCAG AAGATCGTGG AGATCTCGTA CTACAACATG 
AACCGCATCC GCCTGCAGTG GTCACGCATT TGGCAGGTCA TCGGAGATCA TTTCAACAAG 
GTTGGTTGTA ACCCGAATGA AGATGTGGCC ATCTTTGCTG TGGACTCCCT GCGACAACTT 
TCTATGAAGT TTTTAGAGAA AGGAGAACTG GCCAATTTCC GCTTCCAAAA GGACTTTTTA 
AGGCCATTCG AGCACATCGT GAAGAAGAAC AGGTCGCCCA CTATCCGGGA CATGGTGATA 
CGTTGTGTGG CCCAGATGGT CAACTCTCAG GCTGCTAATA TTCGCTCTGG CTGGAAAAAC 
ATCTTCTCTG TGTTTCATCA AGCAGCGTCA GATCATGATG AGAACATTGT GGATCTTGCA 
TTTCAGACCA CAGGGCACAT TGTTATGAAC ACATTTCAGC AGCATTTTGC AGCTGCCATT 
GACTCCTTCC AGGATGCAGT GAAGTGTTTG TCGGAGTTTG TTTGTAACGC TGCGTTTCCT 
GACACCAGTA TGGAAGCCAT TCGGCTAATT CGCCACTGTG CTAAATATGT GTCTGAAAGG 
CCGCAGGCCC TGAGGGAGTA CACTAGTGAT GACATGAACG TGGCCCCTGG TGACCGGGTT 
TGGGTGCGAG GCTGGTTCCC CATCCTGTTT GAGCTTTCCT GCATCATCAA TCGCTGCAAA 
CTTGATGTCA GGACCAGGGG TCTGACCGTC ATGTTTGAGA TCATGAAGAG TTACGGACAC 
ACCTTTGAAA AGCACTGGTG GCATGATCTC TTTCGGATCA TCTTCAGAAT ATTCGACAAC 
ATGAAGCTCC CTGAGCAGCA GACTGAAAAA ACAGAGTGGA TGACGACGAC ATGTAACCAC 
GCTCTCTACG CCATCTGCGA TGTGTTCACG CAGTTCTACG AGCCTCTCAG TGAAGTGCTA 
CTGGCGGACA TTTTTGCGCA GCTGCAGTGG TGCGTCAAAC AAGATAATGA GCAGCTGGCA 
CGCTCGGGCA CGAACTGTCT GGAGAACCTG GTGATCCTGA ACGGGGAGAA GTTCAGTCCG 
GAGGTGTGGG ACGTCACCTG CTCCTGCATG CTGGACATCT TCCAGACCAC CAGCCCTCAT 
GCCTTGCTGA CATGGAGACC AGCTGGACAG GAAGAGGAGG TGGGAGAAGG GAAACACATG 
GATGTTGAGG TGGATTCCCA GTCACAGAGC AGCTTTGAGA GAACTCTGTC AGAGAGAGGC 
CACAGTCAAA TGTCCACCGC CAGCGATGAA ACATGGAAAG GAAAGTCCCA CACCAGGGTT 
TCGGACCACA GGCTGTTTGC AGGGCTGCTT ATAAAGTGTG TCGTACAGCT GGAGTTGATT 
CAGACAATAG ACAACATTGT ATTCTACCCT GCTACCAGCA AGAGAGAAGA TGCTGAGAAC 
ATGGCCGCTG CTCAGCGAGA TGCACTTGAG CAGGCAGAAG AGGGAGAGGC GGAGGTGGAT 
CAGGGCATGT ACAGACACCT GTCATCCCAG CACCTCTTCA AACTGCTCGA CTGCCTCCTC 
GAGTCCCACA GGTTTGCCAA GGACTTCAAC TCCAACAATG AGCAGCGCAC GGCCCTCTGG 
AGAGCAGGCT TTAAGGGGAA GTCAAAACCG AATCTTCTGA AGCAGGAGAC CAGCAGTCTG 
GCCTGCAGTC TAAGGATCCT CTTTCGCATG TACTCCGACA CGCAGCTGCG AGACTCCTGG 
CCAGACATCC AGACACGTCT GCTGCATGTG TGCAGTGAAG CCCTGGCGTA CTTCATCAGT 
CTGACCTCAG AGAGTCATCG TGAAGCGTGG ACGAGTCTCC TGCTGCTGCT GCTCACCCGC 
ACACTCCGAC TGCCTGATGA TAAATTCAAG CCTCATGCCT CCTGCTACTA CCACCATCTA 
TGTGAGATGA TGCAGTTCGA CCTCATTCCG GAGCTCCGCG CTGTTCTGCG GCGATTTTTC 
CTCCGCATCG GTTCCGTCTT CCACATCGCT GCTCCTGAAC TGGGCCAAGC ACAGCCTTCG 
TCATCATCAT CTTCATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arfgef2 ( XM_002666467.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002666513.2 Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (arfgef2), mRNA.

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CloneID	ODa27634
Clone ID Related Accession (Same CDS sequence)	XM_002666467.4 , XM_002666467.5
Accession Version	XM_002666467.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002666467.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAC AGGAAGCGCT GGCAACACAT CGACGGCATA CGCAGACAGA AAGCCTGAGC 
ACCAAGAGCA TGTTTCTGTC TCGGGCGCTG GAGAAAATCC TCGCGGATAA AGAGGTGAAG 
CGGAGCCAGC ACAACCAGCT GCGCAAGGCT TGTCAAGTGG CGCTAGATGA AATCAAGCAT 
GAACTGGAAA AGCAGAAGGA CGGCACTGTT GTTCCTCCCA GAGCCAACTA CATTGAGGCT 
GACAAATATG TCCTGCCTTT TGAGCTGGCA TGTCAATCCA AGTCTCCACG TATCGTCAGC 
ACATCTCTCG ATTGCCTACA GAAACTCATT GCGTATGGGC ACATCACAGG AAACGCCCCT 
GACAGCGCAG CACCAGGGAA GCGACTGATT GACCGTCTGG TGGAGACCAT CTGTAACTGC 
TTTCAGGGAC CCCAGACTGA TGAGGGCGTG CAGCTTCAGA TCATTAAGGC TCTGCTGACT 
GCTGTGACAT CACCACACAT AGAGATCCAT GAGGGGACGA TTCTGCTAAC TGTGCGCACC 
TGCTACAACA TCTACCTGGC CAGCCGTAAT CTGATCAACC AGACCACAGC CAAGGCCACT 
CTCACACAGA TGCTCAATGT CATCTTTACC CGCATGGAGA ACCAGGCGGC TTTAGAAGCT 
CAGGAAGCAG AGAAGGAGCG GCAGCGTTTA CAGCTGCGCA ATCCTTCCCC AGTATCCAGT 
AACCCTTCAC CTGTCAACCA AAATCCTTCT CAAACTCCTG GACGGGGATC GGGATCCCCA 
TGGATGGGAC GAGCCTGCTC TGAACAGAAC GGTTCGCCTT GTCTCAGCCC TCCTGGGGTC 
CCCTCTACTC CCTCCACCCC TCAAGACTCA TCCTCCATCA ACAGCCGGCC AGAGGAGGAG 
TTTGCCACGG ATAAACAGAG CACAAAGGAC ATTACACATC CAGATGAGGA GCTTCAGGAG 
TCAGACCAAA CGACTGTCGA TCCTTCTGTG ACGGGAGGTG TTGAAGGAGA GGAAACGCAT 
CCGCAGTCTG AAACAGAAGC AGGTTCTGAG GTCCTGGAGG AGACAGAGCC GGACAGTGGA 
TTGGGAGACA GCTCTGTGGA AGGAGGACTA GATCAACATT CTGACTCAGA AACCAGAATG 
CCGCCCCCTG TTGTCCGAGT TGACACACAG CAGATGAATG GAATGGTGGA TGACCGCGGC 
TCAGAGTCCT CCACAGACAT GCTGGATGCA GAAAGCATTC ATGGCAGTCA AACAGCTGCG 
CGCTTCTCCC ACATTCTCCA GAAAGATGCT TTCCTTGTGT TTCGCTCGCT CTGCAAGCTC 
TCCATGAAGC CTCTGGCTGA TGGACCACCA GACCCAAAGT CTCATGAGTT GCGCTCTAAG 
GTTGTTTCTC TGCAGCTGTT GCTGTCGGTG CTGCAGGGCG CTGGACCTGT GTTTCGAACC 
CATGAGATGT TCGTGAACGC CATCAAACAG TACCTGTGTG TGGCGCTCTC TAAAAACGGG 
GTCTCCTCTG TGCCTGAAGT ATTTGAACTG TCTCTTGCCA TCTTCCTCAC CTTGCTCTCC 
CACTTCAAAG TGCACCTTAA GATGCAGATC GAGGTGTTCT TCAGGGAGAT CTTTCTGACA 
ATCCTGGAGA CATCGTCCAG CTCTTTTGAG CACAAATGGA TGGTCATTCA GACTCTAACA 
CGAATATGTG CAGACGCACA GTGTGTTGTG GATATTTATG TTAACTATGA TTGTGATCTC 
AACGCTGCAA ACATTTTTGA GCGGCTGGTA AATGACCTGT CTAAAATCGC CCAGGGGAGA 
AGTGGACAGG AGCTGGGCAT GACTCCATTA CAGGAGCTGA GTTTGAGAAA GAAAGGCCTG 
GAGTGTCTGG TGTCTATTTT GAAGTGCATG GTGGAATGGA GCAGAGACAT GTACGTCAAC 
CCCAACCTGC AGGCTAACCT TGGTCAGGAA CGTCCAGCAG AGGGCGACAG TGCTGACGTG 
AAGCTGTCTG AGCATCTCTC CTCTCGCAGA GATAGCGTGA GCTCTCTGGA CTCCACCGTG 
TCGTCTGGAG TTCAGCAATC TCAGCCTGAT CACCCCGAGC AGTATGAGGT CATCAAGCAG 
CAGAAGGAGA TCATCGAGCA TGGCATTGAG CTGTTTAACA AGAAACCAAA AAGGGGATTG 
CAATATCTGC AGGAGCAAGG CATGCTGGGA ACTTCACCAG AGGACATTGC ACAGTTCCTT 
CAACAGGAGG AGCGATTAGA CACGACTCAG GTTGGCGAGT TCCTGGGTGA AAATGTGAAG 
TTTAATAAGG AGGTTATGTA CTGCTACGTG GACCGACTGG ACTTCTGTGG CAAAGACTTT 
GTGTCCGCAC TTCGTGCCTT TTTGGAGGGC TTCAGGCTTC CAGGTGAAGC CCAGAAAATC 
GATCGGCTGA TGGAGAAGTT TGCCGCCCGC TACCTGGAGT GCAATCAGGG GCAGACTCTG 
TTTGCCAGCG CCGACACTGC TTACGTTCTG GCCTACTCCA TCATCATGTT AACCACTGAC 
CTGCACAGTC CACAGGTGAA GAACAAAATG ACGAAGGAGC AGTACATAAA AATGAACCGT 
GGCATTAATG ACAGTAAAGA CCTGCCTGAG GAGTATCTGT CCTCCATCTA CGATGAAATA 
GCAGGGAAGA AGATCGCCAT GAAGGAGAGC AAGGAGTACT CCATCACTCC CAAATCCAGC 
AAGCAGAATG TAGCCAATGA GAAGCAGAGA AGGCTGCTGT ATAACATGGA GATGGAGCAG 
ATGGCTAAAA CCGCTAAAGC TCTGATGGAG GCAGTCAGTC ACGCTCAGGC CCCCTTCTTC 
AGTGCCACTC ACCTAGAGCA CGTCCGGCCC ATGTTCAAGC TGGCCTGGAC TCCCTTGTTG 
GCTGCGTTCA GCGTCGGCCT TCAGGATTGT GACGATCAGG ATGTGGCATC TCTGTGCCTG 
GAGGGGATTC GCTGTGCTAT CAGGATCGCC TGCATCTTTA ATATGCAGTT GGAAAGAGAT 
GCTTATATCC AGGCCTTGGC CCGGTTTACA CTGCTCACTG CCAGCTCAAG TATCACAGAG 
ATGAAGCAGA AGAACATTGA CACCATAAAG ACTCTTATCA TGGTCGCCCA CACTGACGGA 
AACTACCTGG GAAACTCCTG GCATGAGATT CTACGGTGTA TCAGTCAGCT GGAGCTGGCT 
CAGTTGATTG GCACAGGGGT GAAGATGCGT ATCTCCGGTG TGGTCCGGGA CCAGGGAGGC 
GGCATAAAGG GCTTCCCATC AGGCGGAGAG GAATTCATGC CCCTCGGCTT AGGTACTCTG 
GTTGGAGGTC CAGACAAGCG TCAGATGGCT CATATCCAGG AGTCAGTAGG GGAGACCAGC 
TCTCAGAGTG TTGTGGTGGC TGTGGACAGA ATCTTCACTG GATCCACCAG GCTTGACGGA 
AATGCAATCG TGGACTTTGT GCGATGGCTG TGTGCCGTCT CAATGGACGA ACTGGCCTCT 
GCTCACCAGC CCCGCATGTT CAGTCTGCAG AAGATCGTGG AGATCTCGTA CTACAACATG 
AACCGCATCC GCCTGCAGTG GTCACGCATT TGGCAGGTCA TCGGAGATCA TTTCAACAAG 
GTTGGTTGTA ACCCGAATGA AGATGTGGCC ATCTTTGCTG TGGACTCCCT GCGACAACTT 
TCTATGAAGT TTTTAGAGAA AGGAGAACTG GCCAATTTCC GCTTCCAAAA GGACTTTTTA 
AGGCCATTCG AGCACATCGT GAAGAAGAAC AGGTCGCCCA CTATCCGGGA CATGGTGATA 
CGTTGTGTGG CCCAGATGGT CAACTCTCAG GCTGCTAATA TTCGCTCTGG CTGGAAAAAC 
ATCTTCTCTG TGTTTCATCA AGCAGCGTCA GATCATGATG AGAACATTGT GGATCTTGCA 
TTTCAGACCA CAGGGCACAT TGTTATGAAC ACATTTCAGC AGCATTTTGC AGCTGCCATT 
GACTCCTTCC AGGATGCAGT GAAGTGTTTG TCGGAGTTTG TTTGTAACGC TGCGTTTCCT 
GACACCAGTA TGGAAGCCAT TCGGCTAATT CGCCACTGTG CTAAATATGT GTCTGAAAGG 
CCGCAGGCCC TGAGGGAGTA CACTAGTGAT GACATGAACG TGGCCCCTGG TGACCGGGTT 
TGGGTGCGAG GCTGGTTCCC CATCCTGTTT GAGCTTTCCT GCATCATCAA TCGCTGCAAA 
CTTGATGTCA GGACCAGGGG TCTGACCGTC ATGTTTGAGA TCATGAAGAG TTACGGACAC 
ACCTTTGAAA AGCACTGGTG GCATGATCTC TTTCGGATCA TCTTCAGAAT ATTCGACAAC 
ATGAAGCTCC CTGAGCAGCA GACTGAAAAA ACAGAGTGGA TGACGACGAC ATGTAACCAC 
GCTCTCTACG CCATCTGCGA TGTGTTCACG CAGTTCTACG AGCCTCTCAG TGAAGTGCTA 
CTGGCGGACA TTTTTGCGCA GCTGCAGTGG TGCGTCAAAC AAGATAATGA GCAGCTGGCA 
CGCTCGGGCA CGAACTGTCT GGAGAACCTG GTGATCCTGA ACGGGGAGAA GTTCAGTCCG 
GAGGTGTGGG ACGTCACCTG CTCCTGCATG CTGGACATCT TCCAGACCAC CAGCCCTCAT 
GCCTTGCTGA CATGGAGACC AGCTGGACAG GAAGAGGAGG TGGGAGAAGG GAAACACATG 
GATGTTGAGG TGGATTCCCA GTCACAGAGC AGCTTTGAGA GAACTCTGTC AGAGAGAGGC 
CACAGTCAAA TGTCCACCGC CAGCGATGAA ACATGGAAAG GAAAGTCCCA CACCAGGGTT 
TCGGACCACA GGCTGTTTGC AGGGCTGCTT ATAAAGTGTG TCGTACAGCT GGAGTTGATT 
CAGACAATAG ACAACATTGT ATTCTACCCT GCTACCAGCA AGAGAGAAGA TGCTGAGAAC 
ATGGCCGCTG CTCAGCGAGA TGCACTTGAG CAGGCAGAAG AGGGAGAGGC GGAGGTGGAT 
CAGGGCATGT ACAGACACCT GTCATCCCAG CACCTCTTCA AACTGCTCGA CTGCCTCCTC 
GAGTCCCACA GGTTTGCCAA GGACTTCAAC TCCAACAATG AGCAGCGCAC GGCCCTCTGG 
AGAGCAGGCT TTAAGGGGAA GTCAAAACCG AATCTTCTGA AGCAGGAGAC CAGCAGTCTG 
GCCTGCAGTC TAAGGATCCT CTTTCGCATG TACTCCGACA CGCAGCTGCG AGACTCCTGG 
CCAGACATCC AGACACGTCT GCTGCATGTG TGCAGTGAAG CCCTGGCGTA CTTCATCAGT 
CTGACCTCAG AGAGTCATCG TGAAGCGTGG ACGAGTCTCC TGCTGCTGCT GCTCACCCGC 
ACACTCCGAC TGCCTGATGA TAAATTCAAG CCTCATGCCT CCTGCTACTA CCACCATCTA 
TGTGAGATGA TGCAGTTCGA CCTCATTCCG GAGCTCCGCG CTGTTCTGCG GCGATTTTTC 
CTCCGCATCG GTTCCGTCTT CCACATCGCT GCTCCTGAAC TGGGCCAAGC ACAGCCTTCG 
TCATCATCAT CTTCATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002666513.2
CDS	244..5784
Translation	MKEQEALATHRRHTQTESLSTKSMFLSRALEKILADKEVKRSQHNQLRKACQVALDEIKHELEKQKDGTVVPPRANYIEADKYVLPFELACQSKSPRIVSTSLDCLQKLIAYGHITGNAPDSAAPGKRLIDRLVETICNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILLTVRTCYNIYLASRNLINQTTAKATLTQMLNVIFTRMENQAALEAQEAEKERQRLQLRNPSPVSSNPSPVNQNPSQTPGRGSGSPWMGRACSEQNGSPCLSPPGVPSTPSTPQDSSSINSRPEEEFATDKQSTKDITHPDEELQESDQTTVDPSVTGGVEGEETHPQSETEAGSEVLEETEPDSGLGDSSVEGGLDQHSDSETRMPPPVVRVDTQQMNGMVDDRGSESSTDMLDAESIHGSQTAARFSHILQKDAFLVFRSLCKLSMKPLADGPPDPKSHELRSKVVSLQLLLSVLQGAGPVFRTHEMFVNAIKQYLCVALSKNGVSSVPEVFELSLAIFLTLLSHFKVHLKMQIEVFFREIFLTILETSSSSFEHKWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGQELGMTPLQELSLRKKGLECLVSILKCMVEWSRDMYVNPNLQANLGQERPAEGDSADVKLSEHLSSRRDSVSSLDSTVSSGVQQSQPDHPEQYEVIKQQKEIIEHGIELFNKKPKRGLQYLQEQGMLGTSPEDIAQFLQQEERLDTTQVGEFLGENVKFNKEVMYCYVDRLDFCGKDFVSALRAFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYDEIAGKKIAMKESKEYSITPKSSKQNVANEKQRRLLYNMEMEQMAKTAKALMEAVSHAQAPFFSATHLEHVRPMFKLAWTPLLAAFSVGLQDCDDQDVASLCLEGIRCAIRIACIFNMQLERDAYIQALARFTLLTASSSITEMKQKNIDTIKTLIMVAHTDGNYLGNSWHEILRCISQLELAQLIGTGVKMRISGVVRDQGGGIKGFPSGGEEFMPLGLGTLVGGPDKRQMAHIQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASAHQPRMFSLQKIVEISYYNMNRIRLQWSRIWQVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIVKKNRSPTIRDMVIRCVAQMVNSQAANIRSGWKNIFSVFHQAASDHDENIVDLAFQTTGHIVMNTFQQHFAAAIDSFQDAVKCLSEFVCNAAFPDTSMEAIRLIRHCAKYVSERPQALREYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWHDLFRIIFRIFDNMKLPEQQTEKTEWMTTTCNHALYAICDVFTQFYEPLSEVLLADIFAQLQWCVKQDNEQLARSGTNCLENLVILNGEKFSPEVWDVTCSCMLDIFQTTSPHALLTWRPAGQEEEVGEGKHMDVEVDSQSQSSFERTLSERGHSQMSTASDETWKGKSHTRVSDHRLFAGLLIKCVVQLELIQTIDNIVFYPATSKREDAENMAAAQRDALEQAEEGEAEVDQGMYRHLSSQHLFKLLDCLLESHRFAKDFNSNNEQRTALWRAGFKGKSKPNLLKQETSSLACSLRILFRMYSDTQLRDSWPDIQTRLLHVCSEALAYFISLTSESHREAWTSLLLLLLTRTLRLPDDKFKPHASCYYHHLCEMMQFDLIPELRAVLRRFFLRIGSVFHIAAPELGQAQPSSSSSSS

Target ORF information:

RefSeq Version	XM_002666467.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (arfgef2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002666467.5

ORF Insert Sequence:

ATGAAAGAAC AGGAAGCGCT GGCAACACAT CGACGGCATA CGCAGACAGA AAGCCTGAGC 
ACCAAGAGCA TGTTTCTGTC TCGGGCGCTG GAGAAAATCC TCGCGGATAA AGAGGTGAAG 
CGGAGCCAGC ACAACCAGCT GCGCAAGGCT TGTCAAGTGG CGCTAGATGA AATCAAGCAT 
GAACTGGAAA AGCAGAAGGA CGGCACTGTT GTTCCTCCCA GAGCCAACTA CATTGAGGCT 
GACAAATATG TCCTGCCTTT TGAGCTGGCA TGTCAATCCA AGTCTCCACG TATCGTCAGC 
ACATCTCTCG ATTGCCTACA GAAACTCATT GCGTATGGGC ACATCACAGG AAACGCCCCT 
GACAGCGCAG CACCAGGGAA GCGACTGATT GACCGTCTGG TGGAGACCAT CTGTAACTGC 
TTTCAGGGAC CCCAGACTGA TGAGGGCGTG CAGCTTCAGA TCATTAAGGC TCTGCTGACT 
GCTGTGACAT CACCACACAT AGAGATCCAT GAGGGGACGA TTCTGCTAAC TGTGCGCACC 
TGCTACAACA TCTACCTGGC CAGCCGTAAT CTGATCAACC AGACCACAGC CAAGGCCACT 
CTCACACAGA TGCTCAATGT CATCTTTACC CGCATGGAGA ACCAGGCGGC TTTAGAAGCT 
CAGGAAGCAG AGAAGGAGCG GCAGCGTTTA CAGCTGCGCA ATCCTTCCCC AGTATCCAGT 
AACCCTTCAC CTGTCAACCA AAATCCTTCT CAAACTCCTG GACGGGGATC GGGATCCCCA 
TGGATGGGAC GAGCCTGCTC TGAACAGAAC GGTTCGCCTT GTCTCAGCCC TCCTGGGGTC 
CCCTCTACTC CCTCCACCCC TCAAGACTCA TCCTCCATCA ACAGCCGGCC AGAGGAGGAG 
TTTGCCACGG ATAAACAGAG CACAAAGGAC ATTACACATC CAGATGAGGA GCTTCAGGAG 
TCAGACCAAA CGACTGTCGA TCCTTCTGTG ACGGGAGGTG TTGAAGGAGA GGAAACGCAT 
CCGCAGTCTG AAACAGAAGC AGGTTCTGAG GTCCTGGAGG AGACAGAGCC GGACAGTGGA 
TTGGGAGACA GCTCTGTGGA AGGAGGACTA GATCAACATT CTGACTCAGA AACCAGAATG 
CCGCCCCCTG TTGTCCGAGT TGACACACAG CAGATGAATG GAATGGTGGA TGACCGCGGC 
TCAGAGTCCT CCACAGACAT GCTGGATGCA GAAAGCATTC ATGGCAGTCA AACAGCTGCG 
CGCTTCTCCC ACATTCTCCA GAAAGATGCT TTCCTTGTGT TTCGCTCGCT CTGCAAGCTC 
TCCATGAAGC CTCTGGCTGA TGGACCACCA GACCCAAAGT CTCATGAGTT GCGCTCTAAG 
GTTGTTTCTC TGCAGCTGTT GCTGTCGGTG CTGCAGGGCG CTGGACCTGT GTTTCGAACC 
CATGAGATGT TCGTGAACGC CATCAAACAG TACCTGTGTG TGGCGCTCTC TAAAAACGGG 
GTCTCCTCTG TGCCTGAAGT ATTTGAACTG TCTCTTGCCA TCTTCCTCAC CTTGCTCTCC 
CACTTCAAAG TGCACCTTAA GATGCAGATC GAGGTGTTCT TCAGGGAGAT CTTTCTGACA 
ATCCTGGAGA CATCGTCCAG CTCTTTTGAG CACAAATGGA TGGTCATTCA GACTCTAACA 
CGAATATGTG CAGACGCACA GTGTGTTGTG GATATTTATG TTAACTATGA TTGTGATCTC 
AACGCTGCAA ACATTTTTGA GCGGCTGGTA AATGACCTGT CTAAAATCGC CCAGGGGAGA 
AGTGGACAGG AGCTGGGCAT GACTCCATTA CAGGAGCTGA GTTTGAGAAA GAAAGGCCTG 
GAGTGTCTGG TGTCTATTTT GAAGTGCATG GTGGAATGGA GCAGAGACAT GTACGTCAAC 
CCCAACCTGC AGGCTAACCT TGGTCAGGAA CGTCCAGCAG AGGGCGACAG TGCTGACGTG 
AAGCTGTCTG AGCATCTCTC CTCTCGCAGA GATAGCGTGA GCTCTCTGGA CTCCACCGTG 
TCGTCTGGAG TTCAGCAATC TCAGCCTGAT CACCCCGAGC AGTATGAGGT CATCAAGCAG 
CAGAAGGAGA TCATCGAGCA TGGCATTGAG CTGTTTAACA AGAAACCAAA AAGGGGATTG 
CAATATCTGC AGGAGCAAGG CATGCTGGGA ACTTCACCAG AGGACATTGC ACAGTTCCTT 
CAACAGGAGG AGCGATTAGA CACGACTCAG GTTGGCGAGT TCCTGGGTGA AAATGTGAAG 
TTTAATAAGG AGGTTATGTA CTGCTACGTG GACCGACTGG ACTTCTGTGG CAAAGACTTT 
GTGTCCGCAC TTCGTGCCTT TTTGGAGGGC TTCAGGCTTC CAGGTGAAGC CCAGAAAATC 
GATCGGCTGA TGGAGAAGTT TGCCGCCCGC TACCTGGAGT GCAATCAGGG GCAGACTCTG 
TTTGCCAGCG CCGACACTGC TTACGTTCTG GCCTACTCCA TCATCATGTT AACCACTGAC 
CTGCACAGTC CACAGGTGAA GAACAAAATG ACGAAGGAGC AGTACATAAA AATGAACCGT 
GGCATTAATG ACAGTAAAGA CCTGCCTGAG GAGTATCTGT CCTCCATCTA CGATGAAATA 
GCAGGGAAGA AGATCGCCAT GAAGGAGAGC AAGGAGTACT CCATCACTCC CAAATCCAGC 
AAGCAGAATG TAGCCAATGA GAAGCAGAGA AGGCTGCTGT ATAACATGGA GATGGAGCAG 
ATGGCTAAAA CCGCTAAAGC TCTGATGGAG GCAGTCAGTC ACGCTCAGGC CCCCTTCTTC 
AGTGCCACTC ACCTAGAGCA CGTCCGGCCC ATGTTCAAGC TGGCCTGGAC TCCCTTGTTG 
GCTGCGTTCA GCGTCGGCCT TCAGGATTGT GACGATCAGG ATGTGGCATC TCTGTGCCTG 
GAGGGGATTC GCTGTGCTAT CAGGATCGCC TGCATCTTTA ATATGCAGTT GGAAAGAGAT 
GCTTATATCC AGGCCTTGGC CCGGTTTACA CTGCTCACTG CCAGCTCAAG TATCACAGAG 
ATGAAGCAGA AGAACATTGA CACCATAAAG ACTCTTATCA TGGTCGCCCA CACTGACGGA 
AACTACCTGG GAAACTCCTG GCATGAGATT CTACGGTGTA TCAGTCAGCT GGAGCTGGCT 
CAGTTGATTG GCACAGGGGT GAAGATGCGT ATCTCCGGTG TGGTCCGGGA CCAGGGAGGC 
GGCATAAAGG GCTTCCCATC AGGCGGAGAG GAATTCATGC CCCTCGGCTT AGGTACTCTG 
GTTGGAGGTC CAGACAAGCG TCAGATGGCT CATATCCAGG AGTCAGTAGG GGAGACCAGC 
TCTCAGAGTG TTGTGGTGGC TGTGGACAGA ATCTTCACTG GATCCACCAG GCTTGACGGA 
AATGCAATCG TGGACTTTGT GCGATGGCTG TGTGCCGTCT CAATGGACGA ACTGGCCTCT 
GCTCACCAGC CCCGCATGTT CAGTCTGCAG AAGATCGTGG AGATCTCGTA CTACAACATG 
AACCGCATCC GCCTGCAGTG GTCACGCATT TGGCAGGTCA TCGGAGATCA TTTCAACAAG 
GTTGGTTGTA ACCCGAATGA AGATGTGGCC ATCTTTGCTG TGGACTCCCT GCGACAACTT 
TCTATGAAGT TTTTAGAGAA AGGAGAACTG GCCAATTTCC GCTTCCAAAA GGACTTTTTA 
AGGCCATTCG AGCACATCGT GAAGAAGAAC AGGTCGCCCA CTATCCGGGA CATGGTGATA 
CGTTGTGTGG CCCAGATGGT CAACTCTCAG GCTGCTAATA TTCGCTCTGG CTGGAAAAAC 
ATCTTCTCTG TGTTTCATCA AGCAGCGTCA GATCATGATG AGAACATTGT GGATCTTGCA 
TTTCAGACCA CAGGGCACAT TGTTATGAAC ACATTTCAGC AGCATTTTGC AGCTGCCATT 
GACTCCTTCC AGGATGCAGT GAAGTGTTTG TCGGAGTTTG TTTGTAACGC TGCGTTTCCT 
GACACCAGTA TGGAAGCCAT TCGGCTAATT CGCCACTGTG CTAAATATGT GTCTGAAAGG 
CCGCAGGCCC TGAGGGAGTA CACTAGTGAT GACATGAACG TGGCCCCTGG TGACCGGGTT 
TGGGTGCGAG GCTGGTTCCC CATCCTGTTT GAGCTTTCCT GCATCATCAA TCGCTGCAAA 
CTTGATGTCA GGACCAGGGG TCTGACCGTC ATGTTTGAGA TCATGAAGAG TTACGGACAC 
ACCTTTGAAA AGCACTGGTG GCATGATCTC TTTCGGATCA TCTTCAGAAT ATTCGACAAC 
ATGAAGCTCC CTGAGCAGCA GACTGAAAAA ACAGAGTGGA TGACGACGAC ATGTAACCAC 
GCTCTCTACG CCATCTGCGA TGTGTTCACG CAGTTCTACG AGCCTCTCAG TGAAGTGCTA 
CTGGCGGACA TTTTTGCGCA GCTGCAGTGG TGCGTCAAAC AAGATAATGA GCAGCTGGCA 
CGCTCGGGCA CGAACTGTCT GGAGAACCTG GTGATCCTGA ACGGGGAGAA GTTCAGTCCG 
GAGGTGTGGG ACGTCACCTG CTCCTGCATG CTGGACATCT TCCAGACCAC CAGCCCTCAT 
GCCTTGCTGA CATGGAGACC AGCTGGACAG GAAGAGGAGG TGGGAGAAGG GAAACACATG 
GATGTTGAGG TGGATTCCCA GTCACAGAGC AGCTTTGAGA GAACTCTGTC AGAGAGAGGC 
CACAGTCAAA TGTCCACCGC CAGCGATGAA ACATGGAAAG GAAAGTCCCA CACCAGGGTT 
TCGGACCACA GGCTGTTTGC AGGGCTGCTT ATAAAGTGTG TCGTACAGCT GGAGTTGATT 
CAGACAATAG ACAACATTGT ATTCTACCCT GCTACCAGCA AGAGAGAAGA TGCTGAGAAC 
ATGGCCGCTG CTCAGCGAGA TGCACTTGAG CAGGCAGAAG AGGGAGAGGC GGAGGTGGAT 
CAGGGCATGT ACAGACACCT GTCATCCCAG CACCTCTTCA AACTGCTCGA CTGCCTCCTC 
GAGTCCCACA GGTTTGCCAA GGACTTCAAC TCCAACAATG AGCAGCGCAC GGCCCTCTGG 
AGAGCAGGCT TTAAGGGGAA GTCAAAACCG AATCTTCTGA AGCAGGAGAC CAGCAGTCTG 
GCCTGCAGTC TAAGGATCCT CTTTCGCATG TACTCCGACA CGCAGCTGCG AGACTCCTGG 
CCAGACATCC AGACACGTCT GCTGCATGTG TGCAGTGAAG CCCTGGCGTA CTTCATCAGT 
CTGACCTCAG AGAGTCATCG TGAAGCGTGG ACGAGTCTCC TGCTGCTGCT GCTCACCCGC 
ACACTCCGAC TGCCTGATGA TAAATTCAAG CCTCATGCCT CCTGCTACTA CCACCATCTA 
TGTGAGATGA TGCAGTTCGA CCTCATTCCG GAGCTCCGCG CTGTTCTGCG GCGATTTTTC 
CTCCGCATCG GTTCCGTCTT CCACATCGCT GCTCCTGAAC TGGGCCAAGC ACAGCCTTCG 
TCATCATCAT CTTCATCATA A