Arfgef2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arfgef2
Entrez Gene ID	99371
Full Name	ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
Synonyms	AI463430,AW493672,BIG2,E230011G24Rik
General protein information	Preferred Names ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) Names brefeldin A-inhibited guanine nucleotide-exchange protein 2 brefeldin A-inhibited GEP 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H3

mRNA and Protein(s)

mRNA	Protein	Name
XM_030252278.1	XP_030108138.1	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X3
XM_006500483.3	XP_006500546.1	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X1
XM_006500484.3	XP_006500547.1	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X2
XM_006500484.4	XP_006500547.1	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X2
XM_011239875.1	XP_011238177.1	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X3
NM_001085495.2	NP_001078964.1	brefeldin A-inhibited guanine nucleotide-exchange protein 2
XM_006500483.4	XP_006500546.1	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ARFGEF2	XP_534448.1
Arabidopsis thaliana (thale cress)	AT4G38200	NP_195533.2
Pan troglodytes (chimpanzee)	ARFGEF2	XP_001165517.2
	ARFGEF2	XP_002798173.1
Schizosaccharomyces pombe (fission yeast)	sec71	NP_594954.1
Arabidopsis thaliana (thale cress)	AT4G35380	NP_195264.2
Homo sapiens (human)	ARFGEF2	NP_006411.2
Rattus norvegicus (Norway rat)	Arfgef2	NP_851597.2
Danio rerio (zebrafish)	arfgef2	XP_002666513.2
Gallus gallus (chicken)	ARFGEF2	XP_417388.3
Xenopus tropicalis (tropical clawed frog)	arfgef2	XP_002933226.2
Bos taurus (cattle)	ARFGEF2	NP_001192581.1
Mus musculus (house mouse)	Arfgef2	NP_001078964.1

Gene Ontology
Bibliography

Related articles in PubMed

Filamin A regulates neuronal migration through brefeldin A-inhibited guanine exchange factor 2-dependent Arf1 activation.
Zhang J, Neal J, Lian G, Hu J, Lu J, Sheen V
The Journal of neuroscience : the official journal of the Society for Neuroscience33(40)15735-46(2013 Oct)

The Sec7 guanine nucleotide exchange factor GBF1 regulates membrane recruitment of BIG1 and BIG2 guanine nucleotide exchange factors to the trans-Golgi network (TGN).
Lowery J, Szul T, Styers M, Holloway Z, Oorschot V, Klumperman J, Sztul E
The Journal of biological chemistry288(16)11532-45(2013 Apr)

Brefeldin A-inhibited guanine exchange factor 2 regulates filamin A phosphorylation and neuronal migration.
Zhang J, Neal J, Lian G, Shi B, Ferland RJ, Sheen V
The Journal of neuroscience : the official journal of the Society for Neuroscience32(36)12619-29(2012 Sep)

Early embryonic lethality in gene trap mice with disruption of the Arfgef2 gene.
Grzmil P, Enkhbaatar Z, Gundsambuu B, Oidovsambuu O, Yalcin S, Wolf S, Engel W, Neesen J
The International journal of developmental biology54(8-9)1259-66(2010)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

coordinated interactions between actin (through FlnA) and vesicle trafficking (through Big2-Arf) direct the assembly and disassembly of membrane protein complexes required for neuronal migration and neuroependymal integrity.
Title: Filamin A regulates neuronal migration through brefeldin A-inhibited guanine exchange factor 2-dependent Arf1 activation.

an early acting GEF (GBF1) activates ARFs that mediate recruitment of late acting GEFs (BIG1/2) to coordinate coating events within the pre-Golgi/Golgi/TGN continuum.
Title: The Sec7 guanine nucleotide exchange factor GBF1 regulates membrane recruitment of BIG1 and BIG2 guanine nucleotide exchange factors to the trans-Golgi network (TGN).

Big2 inhibition promotes Filamin A phosphorylation, thereby disrupting cell intrinsic neuronal migration.
Title: Brefeldin A-inhibited guanine exchange factor 2 regulates filamin A phosphorylation and neuronal migration.

Results indicate that murine BIG2 is essential for early embryonic development.
Title: Early embryonic lethality in gene trap mice with disruption of the Arfgef2 gene.

The following Arfgef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arfgef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu31077	XM_006500483.4 Latest version!	Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu31078	XM_006500484.4 Latest version!	Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu88086	XM_030252278.1 Latest version!	Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu21343	NM_001085495.2 Latest version!	Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu71969	XM_011239875.1 Latest version!	Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu31078	XM_006500484.3	Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu31077	XM_006500483.3	Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arfgef2 ( XM_006500483.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500546.1 Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31077
Clone ID Related Accession (Same CDS sequence)	XM_006500483.3 , XM_006500483.4
Accession Version	XM_006500483.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006500483.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAAA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCT 
GACAAGGAGG TGAAGCGGCC CCAGCACTCC CAGCTGCGTA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAACT AGAAAAGCAG AGGCTTGGCG CTGCGGCTCC ACCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTCCTT CCATTCGAGC TGGCCTGCCA ATCCAAGTCA 
CCTCGGGTGG TCAGCACATC CCTGGACTGC TTGCAGAAAC TCATCGCATA TGGACACATC 
ACTGGCAATG CCCCTGACAG TGGCGCTCCT GGGAAGCGGC TGATCGACAG GATCGTTGAA 
ACCATTTGCA ATTGTTTTCA GGGCCCTCAG ACAGATGAAG GGGTTCAGCT ACAAATCATT 
AAGGCTCTGC TGACTGCTGT GACATCCCCA CACATTGAAA TCCATGAGGG CACCATCCTG 
CAGACAGTGA GGACCTGCTA CAATATCTAT CTGGCCAGCA AAAACCTCAT CAATCAAACC 
ACCGCCAAGG CCACCCTTAC CCAAATGCTG AATGTCATCT TCACCCGCAT GGAAAACCAA 
GTGTTACAGG AGGCCAGAGA ATTGGAAAAA CCAATGCAGT CAAAACCTCA GTCCCCGGTG 
ATCCAGGCCA CAGCAGGCTC TCCCAAGTTC AGCCGCCTGA AGCAGAGCCA GGCCCAGAGC 
AAACCCACGA CTCCTGAGAA AGCAGAGCTG CCCAATGGGG ACCATGCCCA GAGTGGCCTA 
GGAAAAGTGA GCTTGGAAAA TGGAGAAGCT CCCCGAGAGA GAGGCTCGCC AGTCTCTGGG 
AGAGCCGAGC CTTCGAGGGG CACTGACAGT GGAGCTCAGG AGGTGGTGAA GGACATCTTA 
GAGGACGTGG TCACGTCTGC TGTGAAAGCA GCAGAAAAGC ATGGTCTGCC GGAGCCCGAC 
CGAGCCCTGG GTGCCCTAGA GTGCCAGGAA TGCGCTGTTC CTCCTGGAGT TGATGAAAAC 
TCACAGACCA ACGGCATAGC AGACGACAGG CAGTCGCTAT CCTCCGCAGA TAATCTGGAA 
CCAGATGTTC AAGGACACCA AGTGGCTGCT AGGTTCTCTC ACATTCTACA GAAGGATGCC 
TTCCTGGTGT TCCGCTCCCT GTGCAAGCTG TCCATGAAGC CCCTTGGGGA AGGGCCACCA 
GATCCAAAGT CCCACGAGCT TCGTTCCAAG GTGGTTTCCC TGCAGCTGCT CCTCTCTGTG 
CTGCAGAATG CTGGCCCAGT CTTCAGGAGC CACGAGATGT TCGTCACTGC CATTAAGCAG 
TACCTGTGTG TGGCCCTTTC CAAGAATGGC GTGTCCTCAG TGCCTGACGT CTTCGAACTC 
TCGCTTGCTA TTTTTCTTAC TCTTCTTTCA AACTTCAAGA TGCACTTGAA AATGCAGATA 
GAGGTCTTTT TCAAAGAGAT TTTCCTGAAC ATTTTAGAAA CATCCACAAG TTCTTTTGAG 
CACCGCTGGA TGGTCATTCA GACCCTGACG AGGATTTGTG CAGATGCCCA GTGCGTTGTG 
GATATTTATG TCAACTACGA CTGTGACTTA AATGCTGCTA ACATTTTCGA GCGACTAGTA 
AATGACTTAT CCAAAATTGC TCAGGGAAGA AGTGGACATG AGCTGGGGAT GACACCTCTA 
CAGGAGCTCA GTCTGAGGAA GAAAGGCCTG GAGTGTCTGG TGTCTATTCT CAAGTGCATG 
GTGGAGTGGA GCAAGGACCT GTATGTGAAT CCCAACCACC AGGCTACCCT GGGTCAGGAG 
AGGCTCCCAG ATCAGGAAAT GGGGGACGGA AAAGGCCTTG ACATGGCAAG ACGGTGCAGC 
GTGACATCCG TGGAGTCCAC AGTGTCCTCG GGGACCCAGA CAGCCATTCA GGATGACCCA 
GAGCAGTTTG AGGTCATCAA ACAACAGAAG GAAATCATTG AGCATGGCAT TGAGCTCTTC 
AATAAGAAGC CCAAGCGAGG TATCCAGTTT CTCCAAGAGC AAGGCATGCT GGGAGCAGCT 
GTAGAAGATA TTGCCCAATT TCTGCACCAG GAGGAGCGGC TGGATTCTAC CCAGGTTGGC 
GAGTTTCTGG GAGACAGCAC GAGGTTTAAC AAGGAGGTGA TGTATGCCTA TGTGGACCAA 
CTGGATTTCT GTGAAAAAGA GTTCGTCTCA GCCCTGAGGA CATTCCTGGA GGGCTTCCGC 
CTGCCTGGGG AAGCCCAGAA GATTGACCGG TTAATGGAGA AGTTTGCTGC ACGATACATA 
GAATGTAACC AAGGGCAAAC CCTGTTTGCT AGTGCGGACA CTGCGTATGT CCTGGCGTAC 
TCCATCATCA TGCTGACCAC AGACCTGCAC AGCCCACAGG TAAAGAATAA GATGACAAAA 
GAGCAGTATA TTAAAATGAA CCGGGGCATC AACGACAGCA AAGACCTGCC TGAGGAGTAC 
CTGTCCAGCA TCTATGACGA GATAGAGGGC AAGAAGATAG CGATGAAGGA GACAAAGGAG 
CACACAATTG CGACCAAGTC CACCAAGCAG AGTGTAGCTA GTGAAAAGCA GAGGCGGCTG 
CTGTACAATG TGGAGATGGA GCAGATGGCT AAAACAGCCA AAGCTCTGAT GGAGGCCGTG 
AGCCATGCCA AAGCCCCGTT TACCAGCGCC ACACACTTGG ACCATGTCCG GCCAATGTTC 
AAACTGGTGT GGACGCCGCT GCTGGCAGCC TATAGTATTG GCCTGCAGAA CTGTGATGAC 
ACGGAGGTGG CCTCCCTGTG TTTGGAAGGC ATCAGGTGTG CAGTCCGCAT TGCCTGCATC 
TTTGGAATGC AGCTGGAACG AGATGCCTAT GTTCAGGCCC TTGCTCGCTT TTCCCTGCTG 
ACAGCCAGCT CCAGCATCAC AGAAATGAAG CAGAAAAACA TCGACACCAT AAAGACACTC 
ATCACTGTGG CTCACACCGA TGGCAACTAC CTTGGCAACT CCTGGCATGA GATCTTGAAG 
TGCATCAGCC AGCTGGAGCT TGCTCAGCTG ATAGGAACTG GGGTGAAGAC TCGCTACCTC 
TCTGGCTCTG GGCGGGAACG AGAAGGGAGC CTGAAGGGCC ACAGTCTGGC AGGAGAAGAA 
TTCATGGGTC TTGGCCTCGG TAACTTGGTG AGTGGTGGTG TAGACAAAAG ACAGATGGCC 
AGCTTCCAGG AGTCGGTTGG TGAAACAAGC TCACAGAGTG TGGTTGTCGC AGTTGACAGA 
ATTTTTACTG GCTCTACCAG ACTGGATGGA AATGCAATAG TTGACTTTGT CCGTTGGCTG 
TGTGCTGTGT CCATGGATGA GCTGGCTTCT CCCCACCATC CTCGAATGTT CAGCCTGCAG 
AAGATTGTGG AAATATCATA CTACAACATG AACAGGATCC GGCTGCAGTG GTCTCGGATA 
TGGCATGTCA TTGGAGATCA CTTCAATAAG GTCGGCTGTA ACCCCAATGA AGATGTGGCC 
ATCTTCGCAG TTGACTCTCT GCGGCAGCTC TCCATGAAGT TCCTGGAGAA GGGAGAATTA 
GCCAACTTCC GATTTCAGAA GGATTTTCTG AGGCCATTTG AACATATTAT GAAGAAAAAC 
AGGTCCCCGA CCATCCGGGA CATGGTGATC CGATGCATCG CTCAGATGGT GAGCTCCCAG 
GCAGCCAACA TCCGCTCAGG CTGGAAGAAC ATCTTTGCTG TGTTCCACCA GGCTGCCTCT 
GACCACGATG GGAACATTGT GGAGCTGGCC TTCCAAACCA CAGGCCACAT CGTCTCAACC 
ATCTTCCAGC ACCACTTCCC TGCAGCCATC GACTCCTTCC AGGATGCTGT GAAGTGCTTG 
TCTGAGTTCG CCTGCAATGC AGCTTTCCCT GACACCAGCA TGGAGGCCAT CCGGCTCATC 
CGCTTCTGTG GGAAATACGT CTCAGAGAGG CCTCGGGTGC TGCAAGAGTA CACAAGTGAT 
GATATGAATG TGGCTCCTGG TGACAGGGTC TGGGTCAGAG GCTGGTTTCC CATCCTGTTT 
GAACTCTCCT GCATCATTAA CAGATGCAAG TTAGATGTAC GCACAAGGGG ACTCACGGTC 
ATGTTTGAGA TTATGAAGAG CTATGGCCAC ACCTTTGCAA AGCACTGGTG GCAGGACCTG 
TTCAGAATCG TGTTTCGAAT TTTTGACAAC ATGAAACTCC CTGAGCAACA GTCAGAGAAA 
TCAGAGTGGA TGACGACCAC GTGCAATCAT GCACTGTACG CTATTTGTGA CGTATTCACC 
CAGTTCTACG AAGCTTTGCA TGAAGTGCTT CTCTCTGATG TGTTTGCGCA GCTGCAGTGG 
TGCGTGAAAC AAGATAATGA GCAGTTGGCC CGATCAGGTA CGAACTGCTT AGAGAACCTA 
GTGATATCCA ACGGCGAGAA GTTCAGCCCT GCTGTCTGGG ATGAAACCTG CAATTGCATG 
TTGGATATTT TCAAAACCAC CATCCCACAT GTTTTGCTGA CGTGGAGACC TGCGGGGATG 
GAGGAGGAGG TGTCAGATAG ACATCTGGAT GTGGACCTAG ACCGCCAGTC TTTAAGCAGC 
ATAGACAGAA ACGCCTCCGA GAGAGGACAG AGCCAGCTGT CCAACCCCAC TGACGACAGC 
TGGAAGGGGG CACCGTATGC ACATCAGAAA CTGCTGGCCA GCCTCCTCAT CAAGTGTGTG 
GTTCAGCTGG AGCTGATCCA GACCATTGAC AACATCGTGT TCTACCCTGC CACCAGCAAG 
AAGGAGGATG CGGAGCACAT GGTTGCTGCC CAGCAAGACA CCCTCGATGC AGAGATCCAC 
ATCGAGACGG AGAATCAGGG CATGTACAAG TTCATGTCCT CCCAGCACCT CTTCAAGCTG 
CTGGACTGCC TGCAGGAGTC CCACTCCTTC TCCAAAGCCT TCAACTCCAA CTATGAGCAG 
CGGACTGTCC TCTGGCGAGC TGGCTTCAAG GGCAAGTCCA AACCCAATCT TCTCAAACAA 
GAAACCAGCA GCCTGGCCTG TTGTCTAAGG ATCCTGTTTA GAATGTACGT TGATGAGAAC 
CGCAGGGATT CCTGGGACGA AATACAGCAG CGACTTCTAA GAGTGTGCAG TGAAGCACTT 
GCCTATTTCA TTACTGTGAA CTCCGAGAGC CATCGGGAAG CATGGACGAG CCTCCTGCTG 
CTACTTCTAA CCAAAACCCT CAAGATAAGC GATGAAAAGT TCAAAGCACA CGCGTCAATG 
TACTACCCCT ACCTGTGTGA AATTATGCAG TTTGACCTGA TCCCTGAGCT CCGAGCAGTT 
CTGCGAAAGT TCTTCCTGCG GATAGGCCTG GTGTATAAGA TATGGATTCC AGAAGAGCCG 
TCGCAAGTGC CAGCAGCACT GTCATCGACC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500546.1
CDS	249..5624
Translation	MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAELEKQRLGAAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPMQSKPQSPVIQATAGSPKFSRLKQSQAQSKPTTPEKAELPNGDHAQSGLGKVSLENGEAPRERGSPVSGRAEPSRGTDSGAQEVVKDILEDVVTSAVKAAEKHGLPEPDRALGALECQECAVPPGVDENSQTNGIADDRQSLSSADNLEPDVQGHQVAARFSHILQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRSHEMFVTAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQATLGQERLPDQEMGDGKGLDMARRCSVTSVESTVSSGTQTAIQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGAAVEDIAQFLHQEERLDSTQVGEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYDEIEGKKIAMKETKEHTIATKSTKQSVASEKQRRLLYNVEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHSLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVSTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWWQDLFRIVFRIFDNMKLPEQQSEKSEWMTTTCNHALYAICDVFTQFYEALHEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPAVWDETCNCMLDIFKTTIPHVLLTWRPAGMEEEVSDRHLDVDLDRQSLSSIDRNASERGQSQLSNPTDDSWKGAPYAHQKLLASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDAEIHIETENQGMYKFMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWDEIQQRLLRVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKISDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGLVYKIWIPEEPSQVPAALSSTW

Target ORF information:

RefSeq Version	XM_006500483.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500483.4

ORF Insert Sequence:

ATGCAGGAAA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCT 
GACAAGGAGG TGAAGCGGCC CCAGCACTCC CAGCTGCGTA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAACT AGAAAAGCAG AGGCTTGGCG CTGCGGCTCC ACCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTCCTT CCATTCGAGC TGGCCTGCCA ATCCAAGTCA 
CCTCGGGTGG TCAGCACATC CCTGGACTGC TTGCAGAAAC TCATCGCATA TGGACACATC 
ACTGGCAATG CCCCTGACAG TGGCGCTCCT GGGAAGCGGC TGATCGACAG GATCGTTGAA 
ACCATTTGCA ATTGTTTTCA GGGCCCTCAG ACAGATGAAG GGGTTCAGCT ACAAATCATT 
AAGGCTCTGC TGACTGCTGT GACATCCCCA CACATTGAAA TCCATGAGGG CACCATCCTG 
CAGACAGTGA GGACCTGCTA CAATATCTAT CTGGCCAGCA AAAACCTCAT CAATCAAACC 
ACCGCCAAGG CCACCCTTAC CCAAATGCTG AATGTCATCT TCACCCGCAT GGAAAACCAA 
GTGTTACAGG AGGCCAGAGA ATTGGAAAAA CCAATGCAGT CAAAACCTCA GTCCCCGGTG 
ATCCAGGCCA CAGCAGGCTC TCCCAAGTTC AGCCGCCTGA AGCAGAGCCA GGCCCAGAGC 
AAACCCACGA CTCCTGAGAA AGCAGAGCTG CCCAATGGGG ACCATGCCCA GAGTGGCCTA 
GGAAAAGTGA GCTTGGAAAA TGGAGAAGCT CCCCGAGAGA GAGGCTCGCC AGTCTCTGGG 
AGAGCCGAGC CTTCGAGGGG CACTGACAGT GGAGCTCAGG AGGTGGTGAA GGACATCTTA 
GAGGACGTGG TCACGTCTGC TGTGAAAGCA GCAGAAAAGC ATGGTCTGCC GGAGCCCGAC 
CGAGCCCTGG GTGCCCTAGA GTGCCAGGAA TGCGCTGTTC CTCCTGGAGT TGATGAAAAC 
TCACAGACCA ACGGCATAGC AGACGACAGG CAGTCGCTAT CCTCCGCAGA TAATCTGGAA 
CCAGATGTTC AAGGACACCA AGTGGCTGCT AGGTTCTCTC ACATTCTACA GAAGGATGCC 
TTCCTGGTGT TCCGCTCCCT GTGCAAGCTG TCCATGAAGC CCCTTGGGGA AGGGCCACCA 
GATCCAAAGT CCCACGAGCT TCGTTCCAAG GTGGTTTCCC TGCAGCTGCT CCTCTCTGTG 
CTGCAGAATG CTGGCCCAGT CTTCAGGAGC CACGAGATGT TCGTCACTGC CATTAAGCAG 
TACCTGTGTG TGGCCCTTTC CAAGAATGGC GTGTCCTCAG TGCCTGACGT CTTCGAACTC 
TCGCTTGCTA TTTTTCTTAC TCTTCTTTCA AACTTCAAGA TGCACTTGAA AATGCAGATA 
GAGGTCTTTT TCAAAGAGAT TTTCCTGAAC ATTTTAGAAA CATCCACAAG TTCTTTTGAG 
CACCGCTGGA TGGTCATTCA GACCCTGACG AGGATTTGTG CAGATGCCCA GTGCGTTGTG 
GATATTTATG TCAACTACGA CTGTGACTTA AATGCTGCTA ACATTTTCGA GCGACTAGTA 
AATGACTTAT CCAAAATTGC TCAGGGAAGA AGTGGACATG AGCTGGGGAT GACACCTCTA 
CAGGAGCTCA GTCTGAGGAA GAAAGGCCTG GAGTGTCTGG TGTCTATTCT CAAGTGCATG 
GTGGAGTGGA GCAAGGACCT GTATGTGAAT CCCAACCACC AGGCTACCCT GGGTCAGGAG 
AGGCTCCCAG ATCAGGAAAT GGGGGACGGA AAAGGCCTTG ACATGGCAAG ACGGTGCAGC 
GTGACATCCG TGGAGTCCAC AGTGTCCTCG GGGACCCAGA CAGCCATTCA GGATGACCCA 
GAGCAGTTTG AGGTCATCAA ACAACAGAAG GAAATCATTG AGCATGGCAT TGAGCTCTTC 
AATAAGAAGC CCAAGCGAGG TATCCAGTTT CTCCAAGAGC AAGGCATGCT GGGAGCAGCT 
GTAGAAGATA TTGCCCAATT TCTGCACCAG GAGGAGCGGC TGGATTCTAC CCAGGTTGGC 
GAGTTTCTGG GAGACAGCAC GAGGTTTAAC AAGGAGGTGA TGTATGCCTA TGTGGACCAA 
CTGGATTTCT GTGAAAAAGA GTTCGTCTCA GCCCTGAGGA CATTCCTGGA GGGCTTCCGC 
CTGCCTGGGG AAGCCCAGAA GATTGACCGG TTAATGGAGA AGTTTGCTGC ACGATACATA 
GAATGTAACC AAGGGCAAAC CCTGTTTGCT AGTGCGGACA CTGCGTATGT CCTGGCGTAC 
TCCATCATCA TGCTGACCAC AGACCTGCAC AGCCCACAGG TAAAGAATAA GATGACAAAA 
GAGCAGTATA TTAAAATGAA CCGGGGCATC AACGACAGCA AAGACCTGCC TGAGGAGTAC 
CTGTCCAGCA TCTATGACGA GATAGAGGGC AAGAAGATAG CGATGAAGGA GACAAAGGAG 
CACACAATTG CGACCAAGTC CACCAAGCAG AGTGTAGCTA GTGAAAAGCA GAGGCGGCTG 
CTGTACAATG TGGAGATGGA GCAGATGGCT AAAACAGCCA AAGCTCTGAT GGAGGCCGTG 
AGCCATGCCA AAGCCCCGTT TACCAGCGCC ACACACTTGG ACCATGTCCG GCCAATGTTC 
AAACTGGTGT GGACGCCGCT GCTGGCAGCC TATAGTATTG GCCTGCAGAA CTGTGATGAC 
ACGGAGGTGG CCTCCCTGTG TTTGGAAGGC ATCAGGTGTG CAGTCCGCAT TGCCTGCATC 
TTTGGAATGC AGCTGGAACG AGATGCCTAT GTTCAGGCCC TTGCTCGCTT TTCCCTGCTG 
ACAGCCAGCT CCAGCATCAC AGAAATGAAG CAGAAAAACA TCGACACCAT AAAGACACTC 
ATCACTGTGG CTCACACCGA TGGCAACTAC CTTGGCAACT CCTGGCATGA GATCTTGAAG 
TGCATCAGCC AGCTGGAGCT TGCTCAGCTG ATAGGAACTG GGGTGAAGAC TCGCTACCTC 
TCTGGCTCTG GGCGGGAACG AGAAGGGAGC CTGAAGGGCC ACAGTCTGGC AGGAGAAGAA 
TTCATGGGTC TTGGCCTCGG TAACTTGGTG AGTGGTGGTG TAGACAAAAG ACAGATGGCC 
AGCTTCCAGG AGTCGGTTGG TGAAACAAGC TCACAGAGTG TGGTTGTCGC AGTTGACAGA 
ATTTTTACTG GCTCTACCAG ACTGGATGGA AATGCAATAG TTGACTTTGT CCGTTGGCTG 
TGTGCTGTGT CCATGGATGA GCTGGCTTCT CCCCACCATC CTCGAATGTT CAGCCTGCAG 
AAGATTGTGG AAATATCATA CTACAACATG AACAGGATCC GGCTGCAGTG GTCTCGGATA 
TGGCATGTCA TTGGAGATCA CTTCAATAAG GTCGGCTGTA ACCCCAATGA AGATGTGGCC 
ATCTTCGCAG TTGACTCTCT GCGGCAGCTC TCCATGAAGT TCCTGGAGAA GGGAGAATTA 
GCCAACTTCC GATTTCAGAA GGATTTTCTG AGGCCATTTG AACATATTAT GAAGAAAAAC 
AGGTCCCCGA CCATCCGGGA CATGGTGATC CGATGCATCG CTCAGATGGT GAGCTCCCAG 
GCAGCCAACA TCCGCTCAGG CTGGAAGAAC ATCTTTGCTG TGTTCCACCA GGCTGCCTCT 
GACCACGATG GGAACATTGT GGAGCTGGCC TTCCAAACCA CAGGCCACAT CGTCTCAACC 
ATCTTCCAGC ACCACTTCCC TGCAGCCATC GACTCCTTCC AGGATGCTGT GAAGTGCTTG 
TCTGAGTTCG CCTGCAATGC AGCTTTCCCT GACACCAGCA TGGAGGCCAT CCGGCTCATC 
CGCTTCTGTG GGAAATACGT CTCAGAGAGG CCTCGGGTGC TGCAAGAGTA CACAAGTGAT 
GATATGAATG TGGCTCCTGG TGACAGGGTC TGGGTCAGAG GCTGGTTTCC CATCCTGTTT 
GAACTCTCCT GCATCATTAA CAGATGCAAG TTAGATGTAC GCACAAGGGG ACTCACGGTC 
ATGTTTGAGA TTATGAAGAG CTATGGCCAC ACCTTTGCAA AGCACTGGTG GCAGGACCTG 
TTCAGAATCG TGTTTCGAAT TTTTGACAAC ATGAAACTCC CTGAGCAACA GTCAGAGAAA 
TCAGAGTGGA TGACGACCAC GTGCAATCAT GCACTGTACG CTATTTGTGA CGTATTCACC 
CAGTTCTACG AAGCTTTGCA TGAAGTGCTT CTCTCTGATG TGTTTGCGCA GCTGCAGTGG 
TGCGTGAAAC AAGATAATGA GCAGTTGGCC CGATCAGGTA CGAACTGCTT AGAGAACCTA 
GTGATATCCA ACGGCGAGAA GTTCAGCCCT GCTGTCTGGG ATGAAACCTG CAATTGCATG 
TTGGATATTT TCAAAACCAC CATCCCACAT GTTTTGCTGA CGTGGAGACC TGCGGGGATG 
GAGGAGGAGG TGTCAGATAG ACATCTGGAT GTGGACCTAG ACCGCCAGTC TTTAAGCAGC 
ATAGACAGAA ACGCCTCCGA GAGAGGACAG AGCCAGCTGT CCAACCCCAC TGACGACAGC 
TGGAAGGGGG CACCGTATGC ACATCAGAAA CTGCTGGCCA GCCTCCTCAT CAAGTGTGTG 
GTTCAGCTGG AGCTGATCCA GACCATTGAC AACATCGTGT TCTACCCTGC CACCAGCAAG 
AAGGAGGATG CGGAGCACAT GGTTGCTGCC CAGCAAGACA CCCTCGATGC AGAGATCCAC 
ATCGAGACGG AGAATCAGGG CATGTACAAG TTCATGTCCT CCCAGCACCT CTTCAAGCTG 
CTGGACTGCC TGCAGGAGTC CCACTCCTTC TCCAAAGCCT TCAACTCCAA CTATGAGCAG 
CGGACTGTCC TCTGGCGAGC TGGCTTCAAG GGCAAGTCCA AACCCAATCT TCTCAAACAA 
GAAACCAGCA GCCTGGCCTG TTGTCTAAGG ATCCTGTTTA GAATGTACGT TGATGAGAAC 
CGCAGGGATT CCTGGGACGA AATACAGCAG CGACTTCTAA GAGTGTGCAG TGAAGCACTT 
GCCTATTTCA TTACTGTGAA CTCCGAGAGC CATCGGGAAG CATGGACGAG CCTCCTGCTG 
CTACTTCTAA CCAAAACCCT CAAGATAAGC GATGAAAAGT TCAAAGCACA CGCGTCAATG 
TACTACCCCT ACCTGTGTGA AATTATGCAG TTTGACCTGA TCCCTGAGCT CCGAGCAGTT 
CTGCGAAAGT TCTTCCTGCG GATAGGCCTG GTGTATAAGA TATGGATTCC AGAAGAGCCG 
TCGCAAGTGC CAGCAGCACT GTCATCGACC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgef2 ( XM_006500484.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500547.1 Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X2, mRNA.

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CloneID	OMu31078
Clone ID Related Accession (Same CDS sequence)	XM_006500484.3 , XM_006500484.4
Accession Version	XM_006500484.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006500484.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAAA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCT 
GACAAGGAGG TGAAGCGGCC CCAGCACTCC CAGCTGCGTA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAACT AGAAAAGCAG AGGCTTGGCG CTGCGGCTCC ACCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTCCTT CCATTCGAGC TGGCCTGCCA ATCCAAGTCA 
CCTCGGGTGG TCAGCACATC CCTGGACTGC TTGCAGAAAC TCATCGCATA TGGACACATC 
ACTGGCAATG CCCCTGACAG TGGCGCTCCT GGGAAGCGGC TGATCGACAG GATCGTTGAA 
ACCATTTGCA ATTGTTTTCA GGGCCCTCAG ACAGATGAAG GGGTTCAGCT ACAAATCATT 
AAGGCTCTGC TGACTGCTGT GACATCCCCA CACATTGAAA TCCATGAGGG CACCATCCTG 
CAGACAGTGA GGACCTGCTA CAATATCTAT CTGGCCAGCA AAAACCTCAT CAATCAAACC 
ACCGCCAAGG CCACCCTTAC CCAAATGCTG AATGTCATCT TCACCCGCAT GGAAAACCAA 
GTGTTACAGG AGGCCAGAGA ATTGGAAAAA CCAATGCAGT CAAAACCTCA GTCCCCGGTG 
ATCCAGGCCA CAGCAGGCTC TCCCAAGTTC AGCCGCCTGA AGCAGAGCCA GGCCCAGAGC 
AAACCCACGA CTCCTGAGAA AGCAGAGCTG CCCAATGGGG ACCATGCCCA GAGTGGCCTA 
GGAAAAGTGA GCTTGGAAAA TGGAGAAGCT CCCCGAGAGA GAGGCTCGCC AGTCTCTGGG 
AGAGCCGAGC CTTCGAGGGA AGCAGCAGAA AAGCATGGTC TGCCGGAGCC CGACCGAGCC 
CTGGGTGCCC TAGAGTGCCA GGAATGCGCT GTTCCTCCTG GAGTTGATGA AAACTCACAG 
ACCAACGGCA TAGCAGACGA CAGGCAGTCG CTATCCTCCG CAGATAATCT GGAACCAGAT 
GTTCAAGGAC ACCAAGTGGC TGCTAGGTTC TCTCACATTC TACAGAAGGA TGCCTTCCTG 
GTGTTCCGCT CCCTGTGCAA GCTGTCCATG AAGCCCCTTG GGGAAGGGCC ACCAGATCCA 
AAGTCCCACG AGCTTCGTTC CAAGGTGGTT TCCCTGCAGC TGCTCCTCTC TGTGCTGCAG 
AATGCTGGCC CAGTCTTCAG GAGCCACGAG ATGTTCGTCA CTGCCATTAA GCAGTACCTG 
TGTGTGGCCC TTTCCAAGAA TGGCGTGTCC TCAGTGCCTG ACGTCTTCGA ACTCTCGCTT 
GCTATTTTTC TTACTCTTCT TTCAAACTTC AAGATGCACT TGAAAATGCA GATAGAGGTC 
TTTTTCAAAG AGATTTTCCT GAACATTTTA GAAACATCCA CAAGTTCTTT TGAGCACCGC 
TGGATGGTCA TTCAGACCCT GACGAGGATT TGTGCAGATG CCCAGTGCGT TGTGGATATT 
TATGTCAACT ACGACTGTGA CTTAAATGCT GCTAACATTT TCGAGCGACT AGTAAATGAC 
TTATCCAAAA TTGCTCAGGG AAGAAGTGGA CATGAGCTGG GGATGACACC TCTACAGGAG 
CTCAGTCTGA GGAAGAAAGG CCTGGAGTGT CTGGTGTCTA TTCTCAAGTG CATGGTGGAG 
TGGAGCAAGG ACCTGTATGT GAATCCCAAC CACCAGGCTA CCCTGGGTCA GGAGAGGCTC 
CCAGATCAGG AAATGGGGGA CGGAAAAGGC CTTGACATGG CAAGACGGTG CAGCGTGACA 
TCCGTGGAGT CCACAGTGTC CTCGGGGACC CAGACAGCCA TTCAGGATGA CCCAGAGCAG 
TTTGAGGTCA TCAAACAACA GAAGGAAATC ATTGAGCATG GCATTGAGCT CTTCAATAAG 
AAGCCCAAGC GAGGTATCCA GTTTCTCCAA GAGCAAGGCA TGCTGGGAGC AGCTGTAGAA 
GATATTGCCC AATTTCTGCA CCAGGAGGAG CGGCTGGATT CTACCCAGGT TGGCGAGTTT 
CTGGGAGACA GCACGAGGTT TAACAAGGAG GTGATGTATG CCTATGTGGA CCAACTGGAT 
TTCTGTGAAA AAGAGTTCGT CTCAGCCCTG AGGACATTCC TGGAGGGCTT CCGCCTGCCT 
GGGGAAGCCC AGAAGATTGA CCGGTTAATG GAGAAGTTTG CTGCACGATA CATAGAATGT 
AACCAAGGGC AAACCCTGTT TGCTAGTGCG GACACTGCGT ATGTCCTGGC GTACTCCATC 
ATCATGCTGA CCACAGACCT GCACAGCCCA CAGGTAAAGA ATAAGATGAC AAAAGAGCAG 
TATATTAAAA TGAACCGGGG CATCAACGAC AGCAAAGACC TGCCTGAGGA GTACCTGTCC 
AGCATCTATG ACGAGATAGA GGGCAAGAAG ATAGCGATGA AGGAGACAAA GGAGCACACA 
ATTGCGACCA AGTCCACCAA GCAGAGTGTA GCTAGTGAAA AGCAGAGGCG GCTGCTGTAC 
AATGTGGAGA TGGAGCAGAT GGCTAAAACA GCCAAAGCTC TGATGGAGGC CGTGAGCCAT 
GCCAAAGCCC CGTTTACCAG CGCCACACAC TTGGACCATG TCCGGCCAAT GTTCAAACTG 
GTGTGGACGC CGCTGCTGGC AGCCTATAGT ATTGGCCTGC AGAACTGTGA TGACACGGAG 
GTGGCCTCCC TGTGTTTGGA AGGCATCAGG TGTGCAGTCC GCATTGCCTG CATCTTTGGA 
ATGCAGCTGG AACGAGATGC CTATGTTCAG GCCCTTGCTC GCTTTTCCCT GCTGACAGCC 
AGCTCCAGCA TCACAGAAAT GAAGCAGAAA AACATCGACA CCATAAAGAC ACTCATCACT 
GTGGCTCACA CCGATGGCAA CTACCTTGGC AACTCCTGGC ATGAGATCTT GAAGTGCATC 
AGCCAGCTGG AGCTTGCTCA GCTGATAGGA ACTGGGGTGA AGACTCGCTA CCTCTCTGGC 
TCTGGGCGGG AACGAGAAGG GAGCCTGAAG GGCCACAGTC TGGCAGGAGA AGAATTCATG 
GGTCTTGGCC TCGGTAACTT GGTGAGTGGT GGTGTAGACA AAAGACAGAT GGCCAGCTTC 
CAGGAGTCGG TTGGTGAAAC AAGCTCACAG AGTGTGGTTG TCGCAGTTGA CAGAATTTTT 
ACTGGCTCTA CCAGACTGGA TGGAAATGCA ATAGTTGACT TTGTCCGTTG GCTGTGTGCT 
GTGTCCATGG ATGAGCTGGC TTCTCCCCAC CATCCTCGAA TGTTCAGCCT GCAGAAGATT 
GTGGAAATAT CATACTACAA CATGAACAGG ATCCGGCTGC AGTGGTCTCG GATATGGCAT 
GTCATTGGAG ATCACTTCAA TAAGGTCGGC TGTAACCCCA ATGAAGATGT GGCCATCTTC 
GCAGTTGACT CTCTGCGGCA GCTCTCCATG AAGTTCCTGG AGAAGGGAGA ATTAGCCAAC 
TTCCGATTTC AGAAGGATTT TCTGAGGCCA TTTGAACATA TTATGAAGAA AAACAGGTCC 
CCGACCATCC GGGACATGGT GATCCGATGC ATCGCTCAGA TGGTGAGCTC CCAGGCAGCC 
AACATCCGCT CAGGCTGGAA GAACATCTTT GCTGTGTTCC ACCAGGCTGC CTCTGACCAC 
GATGGGAACA TTGTGGAGCT GGCCTTCCAA ACCACAGGCC ACATCGTCTC AACCATCTTC 
CAGCACCACT TCCCTGCAGC CATCGACTCC TTCCAGGATG CTGTGAAGTG CTTGTCTGAG 
TTCGCCTGCA ATGCAGCTTT CCCTGACACC AGCATGGAGG CCATCCGGCT CATCCGCTTC 
TGTGGGAAAT ACGTCTCAGA GAGGCCTCGG GTGCTGCAAG AGTACACAAG TGATGATATG 
AATGTGGCTC CTGGTGACAG GGTCTGGGTC AGAGGCTGGT TTCCCATCCT GTTTGAACTC 
TCCTGCATCA TTAACAGATG CAAGTTAGAT GTACGCACAA GGGGACTCAC GGTCATGTTT 
GAGATTATGA AGAGCTATGG CCACACCTTT GCAAAGCACT GGTGGCAGGA CCTGTTCAGA 
ATCGTGTTTC GAATTTTTGA CAACATGAAA CTCCCTGAGC AACAGTCAGA GAAATCAGAG 
TGGATGACGA CCACGTGCAA TCATGCACTG TACGCTATTT GTGACGTATT CACCCAGTTC 
TACGAAGCTT TGCATGAAGT GCTTCTCTCT GATGTGTTTG CGCAGCTGCA GTGGTGCGTG 
AAACAAGATA ATGAGCAGTT GGCCCGATCA GGTACGAACT GCTTAGAGAA CCTAGTGATA 
TCCAACGGCG AGAAGTTCAG CCCTGCTGTC TGGGATGAAA CCTGCAATTG CATGTTGGAT 
ATTTTCAAAA CCACCATCCC ACATGTTTTG CTGACGTGGA GACCTGCGGG GATGGAGGAG 
GAGGTGTCAG ATAGACATCT GGATGTGGAC CTAGACCGCC AGTCTTTAAG CAGCATAGAC 
AGAAACGCCT CCGAGAGAGG ACAGAGCCAG CTGTCCAACC CCACTGACGA CAGCTGGAAG 
GGGGCACCGT ATGCACATCA GAAACTGCTG GCCAGCCTCC TCATCAAGTG TGTGGTTCAG 
CTGGAGCTGA TCCAGACCAT TGACAACATC GTGTTCTACC CTGCCACCAG CAAGAAGGAG 
GATGCGGAGC ACATGGTTGC TGCCCAGCAA GACACCCTCG ATGCAGAGAT CCACATCGAG 
ACGGAGAATC AGGGCATGTA CAAGTTCATG TCCTCCCAGC ACCTCTTCAA GCTGCTGGAC 
TGCCTGCAGG AGTCCCACTC CTTCTCCAAA GCCTTCAACT CCAACTATGA GCAGCGGACT 
GTCCTCTGGC GAGCTGGCTT CAAGGGCAAG TCCAAACCCA ATCTTCTCAA ACAAGAAACC 
AGCAGCCTGG CCTGTTGTCT AAGGATCCTG TTTAGAATGT ACGTTGATGA GAACCGCAGG 
GATTCCTGGG ACGAAATACA GCAGCGACTT CTAAGAGTGT GCAGTGAAGC ACTTGCCTAT 
TTCATTACTG TGAACTCCGA GAGCCATCGG GAAGCATGGA CGAGCCTCCT GCTGCTACTT 
CTAACCAAAA CCCTCAAGAT AAGCGATGAA AAGTTCAAAG CACACGCGTC AATGTACTAC 
CCCTACCTGT GTGAAATTAT GCAGTTTGAC CTGATCCCTG AGCTCCGAGC AGTTCTGCGA 
AAGTTCTTCC TGCGGATAGG CCTGGTGTAT AAGATATGGA TTCCAGAAGA GCCGTCGCAA 
GTGCCAGCAG CACTGTCATC GACCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500547.1
CDS	265..5574
Translation	MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAELEKQRLGAAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPMQSKPQSPVIQATAGSPKFSRLKQSQAQSKPTTPEKAELPNGDHAQSGLGKVSLENGEAPRERGSPVSGRAEPSREAAEKHGLPEPDRALGALECQECAVPPGVDENSQTNGIADDRQSLSSADNLEPDVQGHQVAARFSHILQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRSHEMFVTAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQATLGQERLPDQEMGDGKGLDMARRCSVTSVESTVSSGTQTAIQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGAAVEDIAQFLHQEERLDSTQVGEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYDEIEGKKIAMKETKEHTIATKSTKQSVASEKQRRLLYNVEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHSLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVSTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWWQDLFRIVFRIFDNMKLPEQQSEKSEWMTTTCNHALYAICDVFTQFYEALHEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPAVWDETCNCMLDIFKTTIPHVLLTWRPAGMEEEVSDRHLDVDLDRQSLSSIDRNASERGQSQLSNPTDDSWKGAPYAHQKLLASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDAEIHIETENQGMYKFMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWDEIQQRLLRVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKISDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGLVYKIWIPEEPSQVPAALSSTW

Target ORF information:

RefSeq Version	XM_006500484.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500484.4

ORF Insert Sequence:

ATGCAGGAAA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCT 
GACAAGGAGG TGAAGCGGCC CCAGCACTCC CAGCTGCGTA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAACT AGAAAAGCAG AGGCTTGGCG CTGCGGCTCC ACCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTCCTT CCATTCGAGC TGGCCTGCCA ATCCAAGTCA 
CCTCGGGTGG TCAGCACATC CCTGGACTGC TTGCAGAAAC TCATCGCATA TGGACACATC 
ACTGGCAATG CCCCTGACAG TGGCGCTCCT GGGAAGCGGC TGATCGACAG GATCGTTGAA 
ACCATTTGCA ATTGTTTTCA GGGCCCTCAG ACAGATGAAG GGGTTCAGCT ACAAATCATT 
AAGGCTCTGC TGACTGCTGT GACATCCCCA CACATTGAAA TCCATGAGGG CACCATCCTG 
CAGACAGTGA GGACCTGCTA CAATATCTAT CTGGCCAGCA AAAACCTCAT CAATCAAACC 
ACCGCCAAGG CCACCCTTAC CCAAATGCTG AATGTCATCT TCACCCGCAT GGAAAACCAA 
GTGTTACAGG AGGCCAGAGA ATTGGAAAAA CCAATGCAGT CAAAACCTCA GTCCCCGGTG 
ATCCAGGCCA CAGCAGGCTC TCCCAAGTTC AGCCGCCTGA AGCAGAGCCA GGCCCAGAGC 
AAACCCACGA CTCCTGAGAA AGCAGAGCTG CCCAATGGGG ACCATGCCCA GAGTGGCCTA 
GGAAAAGTGA GCTTGGAAAA TGGAGAAGCT CCCCGAGAGA GAGGCTCGCC AGTCTCTGGG 
AGAGCCGAGC CTTCGAGGGA AGCAGCAGAA AAGCATGGTC TGCCGGAGCC CGACCGAGCC 
CTGGGTGCCC TAGAGTGCCA GGAATGCGCT GTTCCTCCTG GAGTTGATGA AAACTCACAG 
ACCAACGGCA TAGCAGACGA CAGGCAGTCG CTATCCTCCG CAGATAATCT GGAACCAGAT 
GTTCAAGGAC ACCAAGTGGC TGCTAGGTTC TCTCACATTC TACAGAAGGA TGCCTTCCTG 
GTGTTCCGCT CCCTGTGCAA GCTGTCCATG AAGCCCCTTG GGGAAGGGCC ACCAGATCCA 
AAGTCCCACG AGCTTCGTTC CAAGGTGGTT TCCCTGCAGC TGCTCCTCTC TGTGCTGCAG 
AATGCTGGCC CAGTCTTCAG GAGCCACGAG ATGTTCGTCA CTGCCATTAA GCAGTACCTG 
TGTGTGGCCC TTTCCAAGAA TGGCGTGTCC TCAGTGCCTG ACGTCTTCGA ACTCTCGCTT 
GCTATTTTTC TTACTCTTCT TTCAAACTTC AAGATGCACT TGAAAATGCA GATAGAGGTC 
TTTTTCAAAG AGATTTTCCT GAACATTTTA GAAACATCCA CAAGTTCTTT TGAGCACCGC 
TGGATGGTCA TTCAGACCCT GACGAGGATT TGTGCAGATG CCCAGTGCGT TGTGGATATT 
TATGTCAACT ACGACTGTGA CTTAAATGCT GCTAACATTT TCGAGCGACT AGTAAATGAC 
TTATCCAAAA TTGCTCAGGG AAGAAGTGGA CATGAGCTGG GGATGACACC TCTACAGGAG 
CTCAGTCTGA GGAAGAAAGG CCTGGAGTGT CTGGTGTCTA TTCTCAAGTG CATGGTGGAG 
TGGAGCAAGG ACCTGTATGT GAATCCCAAC CACCAGGCTA CCCTGGGTCA GGAGAGGCTC 
CCAGATCAGG AAATGGGGGA CGGAAAAGGC CTTGACATGG CAAGACGGTG CAGCGTGACA 
TCCGTGGAGT CCACAGTGTC CTCGGGGACC CAGACAGCCA TTCAGGATGA CCCAGAGCAG 
TTTGAGGTCA TCAAACAACA GAAGGAAATC ATTGAGCATG GCATTGAGCT CTTCAATAAG 
AAGCCCAAGC GAGGTATCCA GTTTCTCCAA GAGCAAGGCA TGCTGGGAGC AGCTGTAGAA 
GATATTGCCC AATTTCTGCA CCAGGAGGAG CGGCTGGATT CTACCCAGGT TGGCGAGTTT 
CTGGGAGACA GCACGAGGTT TAACAAGGAG GTGATGTATG CCTATGTGGA CCAACTGGAT 
TTCTGTGAAA AAGAGTTCGT CTCAGCCCTG AGGACATTCC TGGAGGGCTT CCGCCTGCCT 
GGGGAAGCCC AGAAGATTGA CCGGTTAATG GAGAAGTTTG CTGCACGATA CATAGAATGT 
AACCAAGGGC AAACCCTGTT TGCTAGTGCG GACACTGCGT ATGTCCTGGC GTACTCCATC 
ATCATGCTGA CCACAGACCT GCACAGCCCA CAGGTAAAGA ATAAGATGAC AAAAGAGCAG 
TATATTAAAA TGAACCGGGG CATCAACGAC AGCAAAGACC TGCCTGAGGA GTACCTGTCC 
AGCATCTATG ACGAGATAGA GGGCAAGAAG ATAGCGATGA AGGAGACAAA GGAGCACACA 
ATTGCGACCA AGTCCACCAA GCAGAGTGTA GCTAGTGAAA AGCAGAGGCG GCTGCTGTAC 
AATGTGGAGA TGGAGCAGAT GGCTAAAACA GCCAAAGCTC TGATGGAGGC CGTGAGCCAT 
GCCAAAGCCC CGTTTACCAG CGCCACACAC TTGGACCATG TCCGGCCAAT GTTCAAACTG 
GTGTGGACGC CGCTGCTGGC AGCCTATAGT ATTGGCCTGC AGAACTGTGA TGACACGGAG 
GTGGCCTCCC TGTGTTTGGA AGGCATCAGG TGTGCAGTCC GCATTGCCTG CATCTTTGGA 
ATGCAGCTGG AACGAGATGC CTATGTTCAG GCCCTTGCTC GCTTTTCCCT GCTGACAGCC 
AGCTCCAGCA TCACAGAAAT GAAGCAGAAA AACATCGACA CCATAAAGAC ACTCATCACT 
GTGGCTCACA CCGATGGCAA CTACCTTGGC AACTCCTGGC ATGAGATCTT GAAGTGCATC 
AGCCAGCTGG AGCTTGCTCA GCTGATAGGA ACTGGGGTGA AGACTCGCTA CCTCTCTGGC 
TCTGGGCGGG AACGAGAAGG GAGCCTGAAG GGCCACAGTC TGGCAGGAGA AGAATTCATG 
GGTCTTGGCC TCGGTAACTT GGTGAGTGGT GGTGTAGACA AAAGACAGAT GGCCAGCTTC 
CAGGAGTCGG TTGGTGAAAC AAGCTCACAG AGTGTGGTTG TCGCAGTTGA CAGAATTTTT 
ACTGGCTCTA CCAGACTGGA TGGAAATGCA ATAGTTGACT TTGTCCGTTG GCTGTGTGCT 
GTGTCCATGG ATGAGCTGGC TTCTCCCCAC CATCCTCGAA TGTTCAGCCT GCAGAAGATT 
GTGGAAATAT CATACTACAA CATGAACAGG ATCCGGCTGC AGTGGTCTCG GATATGGCAT 
GTCATTGGAG ATCACTTCAA TAAGGTCGGC TGTAACCCCA ATGAAGATGT GGCCATCTTC 
GCAGTTGACT CTCTGCGGCA GCTCTCCATG AAGTTCCTGG AGAAGGGAGA ATTAGCCAAC 
TTCCGATTTC AGAAGGATTT TCTGAGGCCA TTTGAACATA TTATGAAGAA AAACAGGTCC 
CCGACCATCC GGGACATGGT GATCCGATGC ATCGCTCAGA TGGTGAGCTC CCAGGCAGCC 
AACATCCGCT CAGGCTGGAA GAACATCTTT GCTGTGTTCC ACCAGGCTGC CTCTGACCAC 
GATGGGAACA TTGTGGAGCT GGCCTTCCAA ACCACAGGCC ACATCGTCTC AACCATCTTC 
CAGCACCACT TCCCTGCAGC CATCGACTCC TTCCAGGATG CTGTGAAGTG CTTGTCTGAG 
TTCGCCTGCA ATGCAGCTTT CCCTGACACC AGCATGGAGG CCATCCGGCT CATCCGCTTC 
TGTGGGAAAT ACGTCTCAGA GAGGCCTCGG GTGCTGCAAG AGTACACAAG TGATGATATG 
AATGTGGCTC CTGGTGACAG GGTCTGGGTC AGAGGCTGGT TTCCCATCCT GTTTGAACTC 
TCCTGCATCA TTAACAGATG CAAGTTAGAT GTACGCACAA GGGGACTCAC GGTCATGTTT 
GAGATTATGA AGAGCTATGG CCACACCTTT GCAAAGCACT GGTGGCAGGA CCTGTTCAGA 
ATCGTGTTTC GAATTTTTGA CAACATGAAA CTCCCTGAGC AACAGTCAGA GAAATCAGAG 
TGGATGACGA CCACGTGCAA TCATGCACTG TACGCTATTT GTGACGTATT CACCCAGTTC 
TACGAAGCTT TGCATGAAGT GCTTCTCTCT GATGTGTTTG CGCAGCTGCA GTGGTGCGTG 
AAACAAGATA ATGAGCAGTT GGCCCGATCA GGTACGAACT GCTTAGAGAA CCTAGTGATA 
TCCAACGGCG AGAAGTTCAG CCCTGCTGTC TGGGATGAAA CCTGCAATTG CATGTTGGAT 
ATTTTCAAAA CCACCATCCC ACATGTTTTG CTGACGTGGA GACCTGCGGG GATGGAGGAG 
GAGGTGTCAG ATAGACATCT GGATGTGGAC CTAGACCGCC AGTCTTTAAG CAGCATAGAC 
AGAAACGCCT CCGAGAGAGG ACAGAGCCAG CTGTCCAACC CCACTGACGA CAGCTGGAAG 
GGGGCACCGT ATGCACATCA GAAACTGCTG GCCAGCCTCC TCATCAAGTG TGTGGTTCAG 
CTGGAGCTGA TCCAGACCAT TGACAACATC GTGTTCTACC CTGCCACCAG CAAGAAGGAG 
GATGCGGAGC ACATGGTTGC TGCCCAGCAA GACACCCTCG ATGCAGAGAT CCACATCGAG 
ACGGAGAATC AGGGCATGTA CAAGTTCATG TCCTCCCAGC ACCTCTTCAA GCTGCTGGAC 
TGCCTGCAGG AGTCCCACTC CTTCTCCAAA GCCTTCAACT CCAACTATGA GCAGCGGACT 
GTCCTCTGGC GAGCTGGCTT CAAGGGCAAG TCCAAACCCA ATCTTCTCAA ACAAGAAACC 
AGCAGCCTGG CCTGTTGTCT AAGGATCCTG TTTAGAATGT ACGTTGATGA GAACCGCAGG 
GATTCCTGGG ACGAAATACA GCAGCGACTT CTAAGAGTGT GCAGTGAAGC ACTTGCCTAT 
TTCATTACTG TGAACTCCGA GAGCCATCGG GAAGCATGGA CGAGCCTCCT GCTGCTACTT 
CTAACCAAAA CCCTCAAGAT AAGCGATGAA AAGTTCAAAG CACACGCGTC AATGTACTAC 
CCCTACCTGT GTGAAATTAT GCAGTTTGAC CTGATCCCTG AGCTCCGAGC AGTTCTGCGA 
AAGTTCTTCC TGCGGATAGG CCTGGTGTAT AAGATATGGA TTCCAGAAGA GCCGTCGCAA 
GTGCCAGCAG CACTGTCATC GACCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgef2 ( XM_030252278.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108138.1 Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X3, mRNA.

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CloneID	OMu88086
Clone ID Related Accession (Same CDS sequence)	XM_030252278.1
Accession Version	XM_030252278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAATG TCATCTTCAC CCGCATGGAA AACCAAGTGT TACAGGAGGC CAGAGAATTG 
GAAAAACCAA TGCAGTCAAA ACCTCAGTCC CCGGTGATCC AGGCCACAGC AGGCTCTCCC 
AAGTTCAGCC GCCTGAAGCA GAGCCAGGCC CAGAGCAAAC CCACGACTCC TGAGAAAGCA 
GAGCTGCCCA ATGGGGACCA TGCCCAGAGT GGCCTAGGAA AAGTGAGCTT GGAAAATGGA 
GAAGCTCCCC GAGAGAGAGG CTCGCCAGTC TCTGGGAGAG CCGAGCCTTC GAGGGGCACT 
GACAGTGGAG CTCAGGAGGT GGTGAAGGAC ATCTTAGAGG ACGTGGTCAC GTCTGCTGTG 
AAAGAAGCAG CAGAAAAGCA TGGTCTGCCG GAGCCCGACC GAGCCCTGGG TGCCCTAGAG 
TGCCAGGAAT GCGCTGTTCC TCCTGGAGTT GATGAAAACT CACAGACCAA CGGCATAGCA 
GACGACAGGC AGTCGCTATC CTCCGCAGAT AATCTGGAAC CAGATGTTCA AGGACACCAA 
GTGGCTGCTA GGTTCTCTCA CATTCTACAG AAGGATGCCT TCCTGGTGTT CCGCTCCCTG 
TGCAAGCTGT CCATGAAGCC CCTTGGGGAA GGGCCACCAG ATCCAAAGTC CCACGAGCTT 
CGTTCCAAGG TGGTTTCCCT GCAGCTGCTC CTCTCTGTGC TGCAGAATGC TGGCCCAGTC 
TTCAGGAGCC ACGAGATGTT CGTCACTGCC ATTAAGCAGT ACCTGTGTGT GGCCCTTTCC 
AAGAATGGCG TGTCCTCAGT GCCTGACGTC TTCGAACTCT CGCTTGCTAT TTTTCTTACT 
CTTCTTTCAA ACTTCAAGAT GCACTTGAAA ATGCAGATAG AGGTCTTTTT CAAAGAGATT 
TTCCTGAACA TTTTAGAAAC ATCCACAAGT TCTTTTGAGC ACCGCTGGAT GGTCATTCAG 
ACCCTGACGA GGATTTGTGC AGATGCCCAG TGCGTTGTGG ATATTTATGT CAACTACGAC 
TGTGACTTAA ATGCTGCTAA CATTTTCGAG CGACTAGTAA ATGACTTATC CAAAATTGCT 
CAGGGAAGAA GTGGACATGA GCTGGGGATG ACACCTCTAC AGGAGCTCAG TCTGAGGAAG 
AAAGGCCTGG AGTGTCTGGT GTCTATTCTC AAGTGCATGG TGGAGTGGAG CAAGGACCTG 
TATGTGAATC CCAACCACCA GGCTACCCTG GGTCAGGAGA GGCTCCCAGA TCAGGAAATG 
GGGGACGGAA AAGGCCTTGA CATGGCAAGA CGGTGCAGCG TGACATCCGT GGAGTCCACA 
GTGTCCTCGG GGACCCAGAC AGCCATTCAG GATGACCCAG AGCAGTTTGA GGTCATCAAA 
CAACAGAAGG AAATCATTGA GCATGGCATT GAGCTCTTCA ATAAGAAGCC CAAGCGAGGT 
ATCCAGTTTC TCCAAGAGCA AGGCATGCTG GGAGCAGCTG TAGAAGATAT TGCCCAATTT 
CTGCACCAGG AGGAGCGGCT GGATTCTACC CAGGTTGGCG AGTTTCTGGG AGACAGCACG 
AGGTTTAACA AGGAGGTGAT GTATGCCTAT GTGGACCAAC TGGATTTCTG TGAAAAAGAG 
TTCGTCTCAG CCCTGAGGAC ATTCCTGGAG GGCTTCCGCC TGCCTGGGGA AGCCCAGAAG 
ATTGACCGGT TAATGGAGAA GTTTGCTGCA CGATACATAG AATGTAACCA AGGGCAAACC 
CTGTTTGCTA GTGCGGACAC TGCGTATGTC CTGGCGTACT CCATCATCAT GCTGACCACA 
GACCTGCACA GCCCACAGGT AAAGAATAAG ATGACAAAAG AGCAGTATAT TAAAATGAAC 
CGGGGCATCA ACGACAGCAA AGACCTGCCT GAGGAGTACC TGTCCAGCAT CTATGACGAG 
ATAGAGGGCA AGAAGATAGC GATGAAGGAG ACAAAGGAGC ACACAATTGC GACCAAGTCC 
ACCAAGCAGA GTGTAGCTAG TGAAAAGCAG AGGCGGCTGC TGTACAATGT GGAGATGGAG 
CAGATGGCTA AAACAGCCAA AGCTCTGATG GAGGCCGTGA GCCATGCCAA AGCCCCGTTT 
ACCAGCGCCA CACACTTGGA CCATGTCCGG CCAATGTTCA AACTGGTGTG GACGCCGCTG 
CTGGCAGCCT ATAGTATTGG CCTGCAGAAC TGTGATGACA CGGAGGTGGC CTCCCTGTGT 
TTGGAAGGCA TCAGGTGTGC AGTCCGCATT GCCTGCATCT TTGGAATGCA GCTGGAACGA 
GATGCCTATG TTCAGGCCCT TGCTCGCTTT TCCCTGCTGA CAGCCAGCTC CAGCATCACA 
GAAATGAAGC AGAAAAACAT CGACACCATA AAGACACTCA TCACTGTGGC TCACACCGAT 
GGCAACTACC TTGGCAACTC CTGGCATGAG ATCTTGAAGT GCATCAGCCA GCTGGAGCTT 
GCTCAGCTGA TAGGAACTGG GGTGAAGACT CGCTACCTCT CTGGCTCTGG GCGGGAACGA 
GAAGGGAGCC TGAAGGGCCA CAGTCTGGCA GGAGAAGAAT TCATGGGTCT TGGCCTCGGT 
AACTTGGTGA GTGGTGGTGT AGACAAAAGA CAGATGGCCA GCTTCCAGGA GTCGGTTGGT 
GAAACAAGCT CACAGAGTGT GGTTGTCGCA GTTGACAGAA TTTTTACTGG CTCTACCAGA 
CTGGATGGAA ATGCAATAGT TGACTTTGTC CGTTGGCTGT GTGCTGTGTC CATGGATGAG 
CTGGCTTCTC CCCACCATCC TCGAATGTTC AGCCTGCAGA AGATTGTGGA AATATCATAC 
TACAACATGA ACAGGATCCG GCTGCAGTGG TCTCGGATAT GGCATGTCAT TGGAGATCAC 
TTCAATAAGG TCGGCTGTAA CCCCAATGAA GATGTGGCCA TCTTCGCAGT TGACTCTCTG 
CGGCAGCTCT CCATGAAGTT CCTGGAGAAG GGAGAATTAG CCAACTTCCG ATTTCAGAAG 
GATTTTCTGA GGCCATTTGA ACATATTATG AAGAAAAACA GGTCCCCGAC CATCCGGGAC 
ATGGTGATCC GATGCATCGC TCAGATGGTG AGCTCCCAGG CAGCCAACAT CCGCTCAGGC 
TGGAAGAACA TCTTTGCTGT GTTCCACCAG GCTGCCTCTG ACCACGATGG GAACATTGTG 
GAGCTGGCCT TCCAAACCAC AGGCCACATC GTCTCAACCA TCTTCCAGCA CCACTTCCCT 
GCAGCCATCG ACTCCTTCCA GGATGCTGTG AAGTGCTTGT CTGAGTTCGC CTGCAATGCA 
GCTTTCCCTG ACACCAGCAT GGAGGCCATC CGGCTCATCC GCTTCTGTGG GAAATACGTC 
TCAGAGAGGC CTCGGGTGCT GCAAGAGTAC ACAAGTGATG ATATGAATGT GGCTCCTGGT 
GACAGGGTCT GGGTCAGAGG CTGGTTTCCC ATCCTGTTTG AACTCTCCTG CATCATTAAC 
AGATGCAAGT TAGATGTACG CACAAGGGGA CTCACGGTCA TGTTTGAGAT TATGAAGAGC 
TATGGCCACA CCTTTGCAAA GCACTGGTGG CAGGACCTGT TCAGAATCGT GTTTCGAATT 
TTTGACAACA TGAAACTCCC TGAGCAACAG TCAGAGAAAT CAGAGTGGAT GACGACCACG 
TGCAATCATG CACTGTACGC TATTTGTGAC GTATTCACCC AGTTCTACGA AGCTTTGCAT 
GAAGTGCTTC TCTCTGATGT GTTTGCGCAG CTGCAGTGGT GCGTGAAACA AGATAATGAG 
CAGTTGGCCC GATCAGGTAC GAACTGCTTA GAGAACCTAG TGATATCCAA CGGCGAGAAG 
TTCAGCCCTG CTGTCTGGGA TGAAACCTGC AATTGCATGT TGGATATTTT CAAAACCACC 
ATCCCACATG TTTTGCTGAC GTGGAGACCT GCGGGGATGG AGGAGGAGGT GTCAGATAGA 
CATCTGGATG TGGACCTAGA CCGCCAGTCT TTAAGCAGCA TAGACAGAAA CGCCTCCGAG 
AGAGGACAGA GCCAGCTGTC CAACCCCACT GACGACAGCT GGAAGGGGGC ACCGTATGCA 
CATCAGAAAC TGCTGGCCAG CCTCCTCATC AAGTGTGTGG TTCAGCTGGA GCTGATCCAG 
ACCATTGACA ACATCGTGTT CTACCCTGCC ACCAGCAAGA AGGAGGATGC GGAGCACATG 
GTTGCTGCCC AGCAAGACAC CCTCGATGCA GAGATCCACA TCGAGACGGA GAATCAGGGC 
ATGTACAAGT TCATGTCCTC CCAGCACCTC TTCAAGCTGC TGGACTGCCT GCAGGAGTCC 
CACTCCTTCT CCAAAGCCTT CAACTCCAAC TATGAGCAGC GGACTGTCCT CTGGCGAGCT 
GGCTTCAAGG GCAAGTCCAA ACCCAATCTT CTCAAACAAG AAACCAGCAG CCTGGCCTGT 
TGTCTAAGGA TCCTGTTTAG AATGTACGTT GATGAGAACC GCAGGGATTC CTGGGACGAA 
ATACAGCAGC GACTTCTAAG AGTGTGCAGT GAAGCACTTG CCTATTTCAT TACTGTGAAC 
TCCGAGAGCC ATCGGGAAGC ATGGACGAGC CTCCTGCTGC TACTTCTAAC CAAAACCCTC 
AAGATAAGCG ATGAAAAGTT CAAAGCACAC GCGTCAATGT ACTACCCCTA CCTGTGTGAA 
ATTATGCAGT TTGACCTGAT CCCTGAGCTC CGAGCAGTTC TGCGAAAGTT CTTCCTGCGG 
ATAGGCCTGG TGTATAAGAT ATGGATTCCA GAAGAGCCGT CGCAAGTGCC AGCAGCACTG 
TCATCGACCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108138.1
CDS	657..5471
Translation	MLNVIFTRMENQVLQEARELEKPMQSKPQSPVIQATAGSPKFSRLKQSQAQSKPTTPEKAELPNGDHAQSGLGKVSLENGEAPRERGSPVSGRAEPSRGTDSGAQEVVKDILEDVVTSAVKEAAEKHGLPEPDRALGALECQECAVPPGVDENSQTNGIADDRQSLSSADNLEPDVQGHQVAARFSHILQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRSHEMFVTAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQATLGQERLPDQEMGDGKGLDMARRCSVTSVESTVSSGTQTAIQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGAAVEDIAQFLHQEERLDSTQVGEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYDEIEGKKIAMKETKEHTIATKSTKQSVASEKQRRLLYNVEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHSLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVSTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWWQDLFRIVFRIFDNMKLPEQQSEKSEWMTTTCNHALYAICDVFTQFYEALHEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPAVWDETCNCMLDIFKTTIPHVLLTWRPAGMEEEVSDRHLDVDLDRQSLSSIDRNASERGQSQLSNPTDDSWKGAPYAHQKLLASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDAEIHIETENQGMYKFMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWDEIQQRLLRVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKISDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGLVYKIWIPEEPSQVPAALSSTW

Target ORF information:

RefSeq Version	XM_030252278.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252278.1

ORF Insert Sequence:

ATGCTGAATG TCATCTTCAC CCGCATGGAA AACCAAGTGT TACAGGAGGC CAGAGAATTG 
GAAAAACCAA TGCAGTCAAA ACCTCAGTCC CCGGTGATCC AGGCCACAGC AGGCTCTCCC 
AAGTTCAGCC GCCTGAAGCA GAGCCAGGCC CAGAGCAAAC CCACGACTCC TGAGAAAGCA 
GAGCTGCCCA ATGGGGACCA TGCCCAGAGT GGCCTAGGAA AAGTGAGCTT GGAAAATGGA 
GAAGCTCCCC GAGAGAGAGG CTCGCCAGTC TCTGGGAGAG CCGAGCCTTC GAGGGGCACT 
GACAGTGGAG CTCAGGAGGT GGTGAAGGAC ATCTTAGAGG ACGTGGTCAC GTCTGCTGTG 
AAAGAAGCAG CAGAAAAGCA TGGTCTGCCG GAGCCCGACC GAGCCCTGGG TGCCCTAGAG 
TGCCAGGAAT GCGCTGTTCC TCCTGGAGTT GATGAAAACT CACAGACCAA CGGCATAGCA 
GACGACAGGC AGTCGCTATC CTCCGCAGAT AATCTGGAAC CAGATGTTCA AGGACACCAA 
GTGGCTGCTA GGTTCTCTCA CATTCTACAG AAGGATGCCT TCCTGGTGTT CCGCTCCCTG 
TGCAAGCTGT CCATGAAGCC CCTTGGGGAA GGGCCACCAG ATCCAAAGTC CCACGAGCTT 
CGTTCCAAGG TGGTTTCCCT GCAGCTGCTC CTCTCTGTGC TGCAGAATGC TGGCCCAGTC 
TTCAGGAGCC ACGAGATGTT CGTCACTGCC ATTAAGCAGT ACCTGTGTGT GGCCCTTTCC 
AAGAATGGCG TGTCCTCAGT GCCTGACGTC TTCGAACTCT CGCTTGCTAT TTTTCTTACT 
CTTCTTTCAA ACTTCAAGAT GCACTTGAAA ATGCAGATAG AGGTCTTTTT CAAAGAGATT 
TTCCTGAACA TTTTAGAAAC ATCCACAAGT TCTTTTGAGC ACCGCTGGAT GGTCATTCAG 
ACCCTGACGA GGATTTGTGC AGATGCCCAG TGCGTTGTGG ATATTTATGT CAACTACGAC 
TGTGACTTAA ATGCTGCTAA CATTTTCGAG CGACTAGTAA ATGACTTATC CAAAATTGCT 
CAGGGAAGAA GTGGACATGA GCTGGGGATG ACACCTCTAC AGGAGCTCAG TCTGAGGAAG 
AAAGGCCTGG AGTGTCTGGT GTCTATTCTC AAGTGCATGG TGGAGTGGAG CAAGGACCTG 
TATGTGAATC CCAACCACCA GGCTACCCTG GGTCAGGAGA GGCTCCCAGA TCAGGAAATG 
GGGGACGGAA AAGGCCTTGA CATGGCAAGA CGGTGCAGCG TGACATCCGT GGAGTCCACA 
GTGTCCTCGG GGACCCAGAC AGCCATTCAG GATGACCCAG AGCAGTTTGA GGTCATCAAA 
CAACAGAAGG AAATCATTGA GCATGGCATT GAGCTCTTCA ATAAGAAGCC CAAGCGAGGT 
ATCCAGTTTC TCCAAGAGCA AGGCATGCTG GGAGCAGCTG TAGAAGATAT TGCCCAATTT 
CTGCACCAGG AGGAGCGGCT GGATTCTACC CAGGTTGGCG AGTTTCTGGG AGACAGCACG 
AGGTTTAACA AGGAGGTGAT GTATGCCTAT GTGGACCAAC TGGATTTCTG TGAAAAAGAG 
TTCGTCTCAG CCCTGAGGAC ATTCCTGGAG GGCTTCCGCC TGCCTGGGGA AGCCCAGAAG 
ATTGACCGGT TAATGGAGAA GTTTGCTGCA CGATACATAG AATGTAACCA AGGGCAAACC 
CTGTTTGCTA GTGCGGACAC TGCGTATGTC CTGGCGTACT CCATCATCAT GCTGACCACA 
GACCTGCACA GCCCACAGGT AAAGAATAAG ATGACAAAAG AGCAGTATAT TAAAATGAAC 
CGGGGCATCA ACGACAGCAA AGACCTGCCT GAGGAGTACC TGTCCAGCAT CTATGACGAG 
ATAGAGGGCA AGAAGATAGC GATGAAGGAG ACAAAGGAGC ACACAATTGC GACCAAGTCC 
ACCAAGCAGA GTGTAGCTAG TGAAAAGCAG AGGCGGCTGC TGTACAATGT GGAGATGGAG 
CAGATGGCTA AAACAGCCAA AGCTCTGATG GAGGCCGTGA GCCATGCCAA AGCCCCGTTT 
ACCAGCGCCA CACACTTGGA CCATGTCCGG CCAATGTTCA AACTGGTGTG GACGCCGCTG 
CTGGCAGCCT ATAGTATTGG CCTGCAGAAC TGTGATGACA CGGAGGTGGC CTCCCTGTGT 
TTGGAAGGCA TCAGGTGTGC AGTCCGCATT GCCTGCATCT TTGGAATGCA GCTGGAACGA 
GATGCCTATG TTCAGGCCCT TGCTCGCTTT TCCCTGCTGA CAGCCAGCTC CAGCATCACA 
GAAATGAAGC AGAAAAACAT CGACACCATA AAGACACTCA TCACTGTGGC TCACACCGAT 
GGCAACTACC TTGGCAACTC CTGGCATGAG ATCTTGAAGT GCATCAGCCA GCTGGAGCTT 
GCTCAGCTGA TAGGAACTGG GGTGAAGACT CGCTACCTCT CTGGCTCTGG GCGGGAACGA 
GAAGGGAGCC TGAAGGGCCA CAGTCTGGCA GGAGAAGAAT TCATGGGTCT TGGCCTCGGT 
AACTTGGTGA GTGGTGGTGT AGACAAAAGA CAGATGGCCA GCTTCCAGGA GTCGGTTGGT 
GAAACAAGCT CACAGAGTGT GGTTGTCGCA GTTGACAGAA TTTTTACTGG CTCTACCAGA 
CTGGATGGAA ATGCAATAGT TGACTTTGTC CGTTGGCTGT GTGCTGTGTC CATGGATGAG 
CTGGCTTCTC CCCACCATCC TCGAATGTTC AGCCTGCAGA AGATTGTGGA AATATCATAC 
TACAACATGA ACAGGATCCG GCTGCAGTGG TCTCGGATAT GGCATGTCAT TGGAGATCAC 
TTCAATAAGG TCGGCTGTAA CCCCAATGAA GATGTGGCCA TCTTCGCAGT TGACTCTCTG 
CGGCAGCTCT CCATGAAGTT CCTGGAGAAG GGAGAATTAG CCAACTTCCG ATTTCAGAAG 
GATTTTCTGA GGCCATTTGA ACATATTATG AAGAAAAACA GGTCCCCGAC CATCCGGGAC 
ATGGTGATCC GATGCATCGC TCAGATGGTG AGCTCCCAGG CAGCCAACAT CCGCTCAGGC 
TGGAAGAACA TCTTTGCTGT GTTCCACCAG GCTGCCTCTG ACCACGATGG GAACATTGTG 
GAGCTGGCCT TCCAAACCAC AGGCCACATC GTCTCAACCA TCTTCCAGCA CCACTTCCCT 
GCAGCCATCG ACTCCTTCCA GGATGCTGTG AAGTGCTTGT CTGAGTTCGC CTGCAATGCA 
GCTTTCCCTG ACACCAGCAT GGAGGCCATC CGGCTCATCC GCTTCTGTGG GAAATACGTC 
TCAGAGAGGC CTCGGGTGCT GCAAGAGTAC ACAAGTGATG ATATGAATGT GGCTCCTGGT 
GACAGGGTCT GGGTCAGAGG CTGGTTTCCC ATCCTGTTTG AACTCTCCTG CATCATTAAC 
AGATGCAAGT TAGATGTACG CACAAGGGGA CTCACGGTCA TGTTTGAGAT TATGAAGAGC 
TATGGCCACA CCTTTGCAAA GCACTGGTGG CAGGACCTGT TCAGAATCGT GTTTCGAATT 
TTTGACAACA TGAAACTCCC TGAGCAACAG TCAGAGAAAT CAGAGTGGAT GACGACCACG 
TGCAATCATG CACTGTACGC TATTTGTGAC GTATTCACCC AGTTCTACGA AGCTTTGCAT 
GAAGTGCTTC TCTCTGATGT GTTTGCGCAG CTGCAGTGGT GCGTGAAACA AGATAATGAG 
CAGTTGGCCC GATCAGGTAC GAACTGCTTA GAGAACCTAG TGATATCCAA CGGCGAGAAG 
TTCAGCCCTG CTGTCTGGGA TGAAACCTGC AATTGCATGT TGGATATTTT CAAAACCACC 
ATCCCACATG TTTTGCTGAC GTGGAGACCT GCGGGGATGG AGGAGGAGGT GTCAGATAGA 
CATCTGGATG TGGACCTAGA CCGCCAGTCT TTAAGCAGCA TAGACAGAAA CGCCTCCGAG 
AGAGGACAGA GCCAGCTGTC CAACCCCACT GACGACAGCT GGAAGGGGGC ACCGTATGCA 
CATCAGAAAC TGCTGGCCAG CCTCCTCATC AAGTGTGTGG TTCAGCTGGA GCTGATCCAG 
ACCATTGACA ACATCGTGTT CTACCCTGCC ACCAGCAAGA AGGAGGATGC GGAGCACATG 
GTTGCTGCCC AGCAAGACAC CCTCGATGCA GAGATCCACA TCGAGACGGA GAATCAGGGC 
ATGTACAAGT TCATGTCCTC CCAGCACCTC TTCAAGCTGC TGGACTGCCT GCAGGAGTCC 
CACTCCTTCT CCAAAGCCTT CAACTCCAAC TATGAGCAGC GGACTGTCCT CTGGCGAGCT 
GGCTTCAAGG GCAAGTCCAA ACCCAATCTT CTCAAACAAG AAACCAGCAG CCTGGCCTGT 
TGTCTAAGGA TCCTGTTTAG AATGTACGTT GATGAGAACC GCAGGGATTC CTGGGACGAA 
ATACAGCAGC GACTTCTAAG AGTGTGCAGT GAAGCACTTG CCTATTTCAT TACTGTGAAC 
TCCGAGAGCC ATCGGGAAGC ATGGACGAGC CTCCTGCTGC TACTTCTAAC CAAAACCCTC 
AAGATAAGCG ATGAAAAGTT CAAAGCACAC GCGTCAATGT ACTACCCCTA CCTGTGTGAA 
ATTATGCAGT TTGACCTGAT CCCTGAGCTC CGAGCAGTTC TGCGAAAGTT CTTCCTGCGG 
ATAGGCCTGG TGTATAAGAT ATGGATTCCA GAAGAGCCGT CGCAAGTGCC AGCAGCACTG 
TCATCGACCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgef2 ( NM_001085495.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001078964.1 Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), mRNA.

Price & Availability↑

CloneID	OMu21343
Clone ID Related Accession (Same CDS sequence)	NM_001085495.2
Accession Version	NM_001085495.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591703.14 and AL591911.8. On Feb 13, 2008 this sequence version replaced NM_001085495.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC158012.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCAGGAAA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCT 
GACAAGGAGG TGAAGCGGCC CCAGCACTCC CAGCTGCGTA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAACT AGAAAAGCAG AGGCTTGGCG CTGCGGCTCC ACCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTCCTT CCATTCGAGC TGGCCTGCCA ATCCAAGTCA 
CCTCGGGTGG TCAGCACATC CCTGGACTGC TTGCAGAAAC TCATCGCATA TGGACACATC 
ACTGGCAATG CCCCTGACAG TGGCGCTCCT GGGAAGCGGC TGATCGACAG GATCGTTGAA 
ACCATTTGCA ATTGTTTTCA GGGCCCTCAG ACAGATGAAG GGGTTCAGCT ACAAATCATT 
AAGGCTCTGC TGACTGCTGT GACATCCCCA CACATTGAAA TCCATGAGGG CACCATCCTG 
CAGACAGTGA GGACCTGCTA CAATATCTAT CTGGCCAGCA AAAACCTCAT CAATCAAACC 
ACCGCCAAGG CCACCCTTAC CCAAATGCTG AATGTCATCT TCACCCGCAT GGAAAACCAA 
GTGTTACAGG AGGCCAGAGA ATTGGAAAAA CCAATGCAGT CAAAACCTCA GTCCCCGGTG 
ATCCAGGCCA CAGCAGGCTC TCCCAAGTTC AGCCGCCTGA AGCAGAGCCA GGCCCAGAGC 
AAACCCACGA CTCCTGAGAA AGCAGAGCTG CCCAATGGGG ACCATGCCCA GAGTGGCCTA 
GGAAAAGTGA GCTTGGAAAA TGGAGAAGCT CCCCGAGAGA GAGGCTCGCC AGTCTCTGGG 
AGAGCCGAGC CTTCGAGGGG CACTGACAGT GGAGCTCAGG AGGTGGTGAA GGACATCTTA 
GAGGACGTGG TCACGTCTGC TGTGAAAGAA GCAGCAGAAA AGCATGGTCT GCCGGAGCCC 
GACCGAGCCC TGGGTGCCCT AGAGTGCCAG GAATGCGCTG TTCCTCCTGG AGTTGATGAA 
AACTCACAGA CCAACGGCAT AGCAGACGAC AGGCAGTCGC TATCCTCCGC AGATAATCTG 
GAACCAGATG TTCAAGGACA CCAAGTGGCT GCTAGGTTCT CTCACATTCT ACAGAAGGAT 
GCCTTCCTGG TGTTCCGCTC CCTGTGCAAG CTGTCCATGA AGCCCCTTGG GGAAGGGCCA 
CCAGATCCAA AGTCCCACGA GCTTCGTTCC AAGGTGGTTT CCCTGCAGCT GCTCCTCTCT 
GTGCTGCAGA ATGCTGGCCC AGTCTTCAGG AGCCACGAGA TGTTCGTCAC TGCCATTAAG 
CAGTACCTGT GTGTGGCCCT TTCCAAGAAT GGCGTGTCCT CAGTGCCTGA CGTCTTCGAA 
CTCTCGCTTG CTATTTTTCT TACTCTTCTT TCAAACTTCA AGATGCACTT GAAAATGCAG 
ATAGAGGTCT TTTTCAAAGA GATTTTCCTG AACATTTTAG AAACATCCAC AAGTTCTTTT 
GAGCACCGCT GGATGGTCAT TCAGACCCTG ACGAGGATTT GTGCAGATGC CCAGTGCGTT 
GTGGATATTT ATGTCAACTA CGACTGTGAC TTAAATGCTG CTAACATTTT CGAGCGACTA 
GTAAATGACT TATCCAAAAT TGCTCAGGGA AGAAGTGGAC ATGAGCTGGG GATGACACCT 
CTACAGGAGC TCAGTCTGAG GAAGAAAGGC CTGGAGTGTC TGGTGTCTAT TCTCAAGTGC 
ATGGTGGAGT GGAGCAAGGA CCTGTATGTG AATCCCAACC ACCAGGCTAC CCTGGGTCAG 
GAGAGGCTCC CAGATCAGGA AATGGGGGAC GGAAAAGGCC TTGACATGGC AAGACGGTGC 
AGCGTGACAT CCGTGGAGTC CACAGTGTCC TCGGGGACCC AGACAGCCAT TCAGGATGAC 
CCAGAGCAGT TTGAGGTCAT CAAACAACAG AAGGAAATCA TTGAGCATGG CATTGAGCTC 
TTCAATAAGA AGCCCAAGCG AGGTATCCAG TTTCTCCAAG AGCAAGGCAT GCTGGGAGCA 
GCTGTAGAAG ATATTGCCCA ATTTCTGCAC CAGGAGGAGC GGCTGGATTC TACCCAGGTT 
GGCGAGTTTC TGGGAGACAG CACGAGGTTT AACAAGGAGG TGATGTATGC CTATGTGGAC 
CAACTGGATT TCTGTGAAAA AGAGTTCGTC TCAGCCCTGA GGACATTCCT GGAGGGCTTC 
CGCCTGCCTG GGGAAGCCCA GAAGATTGAC CGGTTAATGG AGAAGTTTGC TGCACGATAC 
ATAGAATGTA ACCAAGGGCA AACCCTGTTT GCTAGTGCGG ACACTGCGTA TGTCCTGGCG 
TACTCCATCA TCATGCTGAC CACAGACCTG CACAGCCCAC AGGTAAAGAA TAAGATGACA 
AAAGAGCAGT ATATTAAAAT GAACCGGGGC ATCAACGACA GCAAAGACCT GCCTGAGGAG 
TACCTGTCCA GCATCTATGA CGAGATAGAG GGCAAGAAGA TAGCGATGAA GGAGACAAAG 
GAGCACACAA TTGCGACCAA GTCCACCAAG CAGAGTGTAG CTAGTGAAAA GCAGAGGCGG 
CTGCTGTACA ATGTGGAGAT GGAGCAGATG GCTAAAACAG CCAAAGCTCT GATGGAGGCC 
GTGAGCCATG CCAAAGCCCC GTTTACCAGC GCCACACACT TGGACCATGT CCGGCCAATG 
TTCAAACTGG TGTGGACGCC GCTGCTGGCA GCCTATAGTA TTGGCCTGCA GAACTGTGAT 
GACACGGAGG TGGCCTCCCT GTGTTTGGAA GGCATCAGGT GTGCAGTCCG CATTGCCTGC 
ATCTTTGGAA TGCAGCTGGA ACGAGATGCC TATGTTCAGG CCCTTGCTCG CTTTTCCCTG 
CTGACAGCCA GCTCCAGCAT CACAGAAATG AAGCAGAAAA ACATCGACAC CATAAAGACA 
CTCATCACTG TGGCTCACAC CGATGGCAAC TACCTTGGCA ACTCCTGGCA TGAGATCTTG 
AAGTGCATCA GCCAGCTGGA GCTTGCTCAG CTGATAGGAA CTGGGGTGAA GACTCGCTAC 
CTCTCTGGCT CTGGGCGGGA ACGAGAAGGG AGCCTGAAGG GCCACAGTCT GGCAGGAGAA 
GAATTCATGG GTCTTGGCCT CGGTAACTTG GTGAGTGGTG GTGTAGACAA AAGACAGATG 
GCCAGCTTCC AGGAGTCGGT TGGTGAAACA AGCTCACAGA GTGTGGTTGT CGCAGTTGAC 
AGAATTTTTA CTGGCTCTAC CAGACTGGAT GGAAATGCAA TAGTTGACTT TGTCCGTTGG 
CTGTGTGCTG TGTCCATGGA TGAGCTGGCT TCTCCCCACC ATCCTCGAAT GTTCAGCCTG 
CAGAAGATTG TGGAAATATC ATACTACAAC ATGAACAGGA TCCGGCTGCA GTGGTCTCGG 
ATATGGCATG TCATTGGAGA TCACTTCAAT AAGGTCGGCT GTAACCCCAA TGAAGATGTG 
GCCATCTTCG CAGTTGACTC TCTGCGGCAG CTCTCCATGA AGTTCCTGGA GAAGGGAGAA 
TTAGCCAACT TCCGATTTCA GAAGGATTTT CTGAGGCCAT TTGAACATAT TATGAAGAAA 
AACAGGTCCC CGACCATCCG GGACATGGTG ATCCGATGCA TCGCTCAGAT GGTGAGCTCC 
CAGGCAGCCA ACATCCGCTC AGGCTGGAAG AACATCTTTG CTGTGTTCCA CCAGGCTGCC 
TCTGACCACG ATGGGAACAT TGTGGAGCTG GCCTTCCAAA CCACAGGCCA CATCGTCTCA 
ACCATCTTCC AGCACCACTT CCCTGCAGCC ATCGACTCCT TCCAGGATGC TGTGAAGTGC 
TTGTCTGAGT TCGCCTGCAA TGCAGCTTTC CCTGACACCA GCATGGAGGC CATCCGGCTC 
ATCCGCTTCT GTGGGAAATA CGTCTCAGAG AGGCCTCGGG TGCTGCAAGA GTACACAAGT 
GATGATATGA ATGTGGCTCC TGGTGACAGG GTCTGGGTCA GAGGCTGGTT TCCCATCCTG 
TTTGAACTCT CCTGCATCAT TAACAGATGC AAGTTAGATG TACGCACAAG GGGACTCACG 
GTCATGTTTG AGATTATGAA GAGCTATGGC CACACCTTTG CAAAGCACTG GTGGCAGGAC 
CTGTTCAGAA TCGTGTTTCG AATTTTTGAC AACATGAAAC TCCCTGAGCA ACAGTCAGAG 
AAATCAGAGT GGATGACGAC CACGTGCAAT CATGCACTGT ACGCTATTTG TGACGTATTC 
ACCCAGTTCT ACGAAGCTTT GCATGAAGTG CTTCTCTCTG ATGTGTTTGC GCAGCTGCAG 
TGGTGCGTGA AACAAGATAA TGAGCAGTTG GCCCGATCAG GTACGAACTG CTTAGAGAAC 
CTAGTGATAT CCAACGGCGA GAAGTTCAGC CCTGCTGTCT GGGATGAAAC CTGCAATTGC 
ATGTTGGATA TTTTCAAAAC CACCATCCCA CATGTTTTGC TGACGTGGAG ACCTGCGGGG 
ATGGAGGAGG AGGTGTCAGA TAGACATCTG GATGTGGACC TAGACCGCCA GTCTTTAAGC 
AGCATAGACA GAAACGCCTC CGAGAGAGGA CAGAGCCAGC TGTCCAACCC CACTGACGAC 
AGCTGGAAGG GGGCACCGTA TGCACATCAG AAACTGCTGG CCAGCCTCCT CATCAAGTGT 
GTGGTTCAGC TGGAGCTGAT CCAGACCATT GACAACATCG TGTTCTACCC TGCCACCAGC 
AAGAAGGAGG ATGCGGAGCA CATGGTTGCT GCCCAGCAAG ACACCCTCGA TGCAGAGATC 
CACATCGAGA CGGAGAATCA GGGCATGTAC AAGTTCATGT CCTCCCAGCA CCTCTTCAAG 
CTGCTGGACT GCCTGCAGGA GTCCCACTCC TTCTCCAAAG CCTTCAACTC CAACTATGAG 
CAGCGGACTG TCCTCTGGCG AGCTGGCTTC AAGGGCAAGT CCAAACCCAA TCTTCTCAAA 
CAAGAAACCA GCAGCCTGGC CTGTTGTCTA AGGATCCTGT TTAGAATGTA CGTTGATGAG 
AACCGCAGGG ATTCCTGGGA CGAAATACAG CAGCGACTTC TAAGAGTGTG CAGTGAAGCA 
CTTGCCTATT TCATTACTGT GAACTCCGAG AGCCATCGGG AAGCATGGAC GAGCCTCCTG 
CTGCTACTTC TAACCAAAAC CCTCAAGATA AGCGATGAAA AGTTCAAAGC ACACGCGTCA 
ATGTACTACC CCTACCTGTG TGAAATTATG CAGTTTGACC TGATCCCTGA GCTCCGAGCA 
GTTCTGCGAA AGTTCTTCCT GCGGATAGGC CTGGTGTATA AGATATGGAT TCCAGAAGAG 
CCGTCGCAAG TGCCAGCAGC ACTGTCATCG ACCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078964.1
CDS	135..5513
Translation	MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAELEKQRLGAAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPMQSKPQSPVIQATAGSPKFSRLKQSQAQSKPTTPEKAELPNGDHAQSGLGKVSLENGEAPRERGSPVSGRAEPSRGTDSGAQEVVKDILEDVVTSAVKEAAEKHGLPEPDRALGALECQECAVPPGVDENSQTNGIADDRQSLSSADNLEPDVQGHQVAARFSHILQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRSHEMFVTAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQATLGQERLPDQEMGDGKGLDMARRCSVTSVESTVSSGTQTAIQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGAAVEDIAQFLHQEERLDSTQVGEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYDEIEGKKIAMKETKEHTIATKSTKQSVASEKQRRLLYNVEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHSLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVSTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWWQDLFRIVFRIFDNMKLPEQQSEKSEWMTTTCNHALYAICDVFTQFYEALHEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPAVWDETCNCMLDIFKTTIPHVLLTWRPAGMEEEVSDRHLDVDLDRQSLSSIDRNASERGQSQLSNPTDDSWKGAPYAHQKLLASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDAEIHIETENQGMYKFMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWDEIQQRLLRVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKISDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGLVYKIWIPEEPSQVPAALSSTW

Target ORF information:

RefSeq Version	NM_001085495.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085495.2

ORF Insert Sequence:

ATGCAGGAAA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCT 
GACAAGGAGG TGAAGCGGCC CCAGCACTCC CAGCTGCGTA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAACT AGAAAAGCAG AGGCTTGGCG CTGCGGCTCC ACCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTCCTT CCATTCGAGC TGGCCTGCCA ATCCAAGTCA 
CCTCGGGTGG TCAGCACATC CCTGGACTGC TTGCAGAAAC TCATCGCATA TGGACACATC 
ACTGGCAATG CCCCTGACAG TGGCGCTCCT GGGAAGCGGC TGATCGACAG GATCGTTGAA 
ACCATTTGCA ATTGTTTTCA GGGCCCTCAG ACAGATGAAG GGGTTCAGCT ACAAATCATT 
AAGGCTCTGC TGACTGCTGT GACATCCCCA CACATTGAAA TCCATGAGGG CACCATCCTG 
CAGACAGTGA GGACCTGCTA CAATATCTAT CTGGCCAGCA AAAACCTCAT CAATCAAACC 
ACCGCCAAGG CCACCCTTAC CCAAATGCTG AATGTCATCT TCACCCGCAT GGAAAACCAA 
GTGTTACAGG AGGCCAGAGA ATTGGAAAAA CCAATGCAGT CAAAACCTCA GTCCCCGGTG 
ATCCAGGCCA CAGCAGGCTC TCCCAAGTTC AGCCGCCTGA AGCAGAGCCA GGCCCAGAGC 
AAACCCACGA CTCCTGAGAA AGCAGAGCTG CCCAATGGGG ACCATGCCCA GAGTGGCCTA 
GGAAAAGTGA GCTTGGAAAA TGGAGAAGCT CCCCGAGAGA GAGGCTCGCC AGTCTCTGGG 
AGAGCCGAGC CTTCGAGGGG CACTGACAGT GGAGCTCAGG AGGTGGTGAA GGACATCTTA 
GAGGACGTGG TCACGTCTGC TGTGAAAGAA GCAGCAGAAA AGCATGGTCT GCCGGAGCCC 
GACCGAGCCC TGGGTGCCCT AGAGTGCCAG GAATGCGCTG TTCCTCCTGG AGTTGATGAA 
AACTCACAGA CCAACGGCAT AGCAGACGAC AGGCAGTCGC TATCCTCCGC AGATAATCTG 
GAACCAGATG TTCAAGGACA CCAAGTGGCT GCTAGGTTCT CTCACATTCT ACAGAAGGAT 
GCCTTCCTGG TGTTCCGCTC CCTGTGCAAG CTGTCCATGA AGCCCCTTGG GGAAGGGCCA 
CCAGATCCAA AGTCCCACGA GCTTCGTTCC AAGGTGGTTT CCCTGCAGCT GCTCCTCTCT 
GTGCTGCAGA ATGCTGGCCC AGTCTTCAGG AGCCACGAGA TGTTCGTCAC TGCCATTAAG 
CAGTACCTGT GTGTGGCCCT TTCCAAGAAT GGCGTGTCCT CAGTGCCTGA CGTCTTCGAA 
CTCTCGCTTG CTATTTTTCT TACTCTTCTT TCAAACTTCA AGATGCACTT GAAAATGCAG 
ATAGAGGTCT TTTTCAAAGA GATTTTCCTG AACATTTTAG AAACATCCAC AAGTTCTTTT 
GAGCACCGCT GGATGGTCAT TCAGACCCTG ACGAGGATTT GTGCAGATGC CCAGTGCGTT 
GTGGATATTT ATGTCAACTA CGACTGTGAC TTAAATGCTG CTAACATTTT CGAGCGACTA 
GTAAATGACT TATCCAAAAT TGCTCAGGGA AGAAGTGGAC ATGAGCTGGG GATGACACCT 
CTACAGGAGC TCAGTCTGAG GAAGAAAGGC CTGGAGTGTC TGGTGTCTAT TCTCAAGTGC 
ATGGTGGAGT GGAGCAAGGA CCTGTATGTG AATCCCAACC ACCAGGCTAC CCTGGGTCAG 
GAGAGGCTCC CAGATCAGGA AATGGGGGAC GGAAAAGGCC TTGACATGGC AAGACGGTGC 
AGCGTGACAT CCGTGGAGTC CACAGTGTCC TCGGGGACCC AGACAGCCAT TCAGGATGAC 
CCAGAGCAGT TTGAGGTCAT CAAACAACAG AAGGAAATCA TTGAGCATGG CATTGAGCTC 
TTCAATAAGA AGCCCAAGCG AGGTATCCAG TTTCTCCAAG AGCAAGGCAT GCTGGGAGCA 
GCTGTAGAAG ATATTGCCCA ATTTCTGCAC CAGGAGGAGC GGCTGGATTC TACCCAGGTT 
GGCGAGTTTC TGGGAGACAG CACGAGGTTT AACAAGGAGG TGATGTATGC CTATGTGGAC 
CAACTGGATT TCTGTGAAAA AGAGTTCGTC TCAGCCCTGA GGACATTCCT GGAGGGCTTC 
CGCCTGCCTG GGGAAGCCCA GAAGATTGAC CGGTTAATGG AGAAGTTTGC TGCACGATAC 
ATAGAATGTA ACCAAGGGCA AACCCTGTTT GCTAGTGCGG ACACTGCGTA TGTCCTGGCG 
TACTCCATCA TCATGCTGAC CACAGACCTG CACAGCCCAC AGGTAAAGAA TAAGATGACA 
AAAGAGCAGT ATATTAAAAT GAACCGGGGC ATCAACGACA GCAAAGACCT GCCTGAGGAG 
TACCTGTCCA GCATCTATGA CGAGATAGAG GGCAAGAAGA TAGCGATGAA GGAGACAAAG 
GAGCACACAA TTGCGACCAA GTCCACCAAG CAGAGTGTAG CTAGTGAAAA GCAGAGGCGG 
CTGCTGTACA ATGTGGAGAT GGAGCAGATG GCTAAAACAG CCAAAGCTCT GATGGAGGCC 
GTGAGCCATG CCAAAGCCCC GTTTACCAGC GCCACACACT TGGACCATGT CCGGCCAATG 
TTCAAACTGG TGTGGACGCC GCTGCTGGCA GCCTATAGTA TTGGCCTGCA GAACTGTGAT 
GACACGGAGG TGGCCTCCCT GTGTTTGGAA GGCATCAGGT GTGCAGTCCG CATTGCCTGC 
ATCTTTGGAA TGCAGCTGGA ACGAGATGCC TATGTTCAGG CCCTTGCTCG CTTTTCCCTG 
CTGACAGCCA GCTCCAGCAT CACAGAAATG AAGCAGAAAA ACATCGACAC CATAAAGACA 
CTCATCACTG TGGCTCACAC CGATGGCAAC TACCTTGGCA ACTCCTGGCA TGAGATCTTG 
AAGTGCATCA GCCAGCTGGA GCTTGCTCAG CTGATAGGAA CTGGGGTGAA GACTCGCTAC 
CTCTCTGGCT CTGGGCGGGA ACGAGAAGGG AGCCTGAAGG GCCACAGTCT GGCAGGAGAA 
GAATTCATGG GTCTTGGCCT CGGTAACTTG GTGAGTGGTG GTGTAGACAA AAGACAGATG 
GCCAGCTTCC AGGAGTCGGT TGGTGAAACA AGCTCACAGA GTGTGGTTGT CGCAGTTGAC 
AGAATTTTTA CTGGCTCTAC CAGACTGGAT GGAAATGCAA TAGTTGACTT TGTCCGTTGG 
CTGTGTGCTG TGTCCATGGA TGAGCTGGCT TCTCCCCACC ATCCTCGAAT GTTCAGCCTG 
CAGAAGATTG TGGAAATATC ATACTACAAC ATGAACAGGA TCCGGCTGCA GTGGTCTCGG 
ATATGGCATG TCATTGGAGA TCACTTCAAT AAGGTCGGCT GTAACCCCAA TGAAGATGTG 
GCCATCTTCG CAGTTGACTC TCTGCGGCAG CTCTCCATGA AGTTCCTGGA GAAGGGAGAA 
TTAGCCAACT TCCGATTTCA GAAGGATTTT CTGAGGCCAT TTGAACATAT TATGAAGAAA 
AACAGGTCCC CGACCATCCG GGACATGGTG ATCCGATGCA TCGCTCAGAT GGTGAGCTCC 
CAGGCAGCCA ACATCCGCTC AGGCTGGAAG AACATCTTTG CTGTGTTCCA CCAGGCTGCC 
TCTGACCACG ATGGGAACAT TGTGGAGCTG GCCTTCCAAA CCACAGGCCA CATCGTCTCA 
ACCATCTTCC AGCACCACTT CCCTGCAGCC ATCGACTCCT TCCAGGATGC TGTGAAGTGC 
TTGTCTGAGT TCGCCTGCAA TGCAGCTTTC CCTGACACCA GCATGGAGGC CATCCGGCTC 
ATCCGCTTCT GTGGGAAATA CGTCTCAGAG AGGCCTCGGG TGCTGCAAGA GTACACAAGT 
GATGATATGA ATGTGGCTCC TGGTGACAGG GTCTGGGTCA GAGGCTGGTT TCCCATCCTG 
TTTGAACTCT CCTGCATCAT TAACAGATGC AAGTTAGATG TACGCACAAG GGGACTCACG 
GTCATGTTTG AGATTATGAA GAGCTATGGC CACACCTTTG CAAAGCACTG GTGGCAGGAC 
CTGTTCAGAA TCGTGTTTCG AATTTTTGAC AACATGAAAC TCCCTGAGCA ACAGTCAGAG 
AAATCAGAGT GGATGACGAC CACGTGCAAT CATGCACTGT ACGCTATTTG TGACGTATTC 
ACCCAGTTCT ACGAAGCTTT GCATGAAGTG CTTCTCTCTG ATGTGTTTGC GCAGCTGCAG 
TGGTGCGTGA AACAAGATAA TGAGCAGTTG GCCCGATCAG GTACGAACTG CTTAGAGAAC 
CTAGTGATAT CCAACGGCGA GAAGTTCAGC CCTGCTGTCT GGGATGAAAC CTGCAATTGC 
ATGTTGGATA TTTTCAAAAC CACCATCCCA CATGTTTTGC TGACGTGGAG ACCTGCGGGG 
ATGGAGGAGG AGGTGTCAGA TAGACATCTG GATGTGGACC TAGACCGCCA GTCTTTAAGC 
AGCATAGACA GAAACGCCTC CGAGAGAGGA CAGAGCCAGC TGTCCAACCC CACTGACGAC 
AGCTGGAAGG GGGCACCGTA TGCACATCAG AAACTGCTGG CCAGCCTCCT CATCAAGTGT 
GTGGTTCAGC TGGAGCTGAT CCAGACCATT GACAACATCG TGTTCTACCC TGCCACCAGC 
AAGAAGGAGG ATGCGGAGCA CATGGTTGCT GCCCAGCAAG ACACCCTCGA TGCAGAGATC 
CACATCGAGA CGGAGAATCA GGGCATGTAC AAGTTCATGT CCTCCCAGCA CCTCTTCAAG 
CTGCTGGACT GCCTGCAGGA GTCCCACTCC TTCTCCAAAG CCTTCAACTC CAACTATGAG 
CAGCGGACTG TCCTCTGGCG AGCTGGCTTC AAGGGCAAGT CCAAACCCAA TCTTCTCAAA 
CAAGAAACCA GCAGCCTGGC CTGTTGTCTA AGGATCCTGT TTAGAATGTA CGTTGATGAG 
AACCGCAGGG ATTCCTGGGA CGAAATACAG CAGCGACTTC TAAGAGTGTG CAGTGAAGCA 
CTTGCCTATT TCATTACTGT GAACTCCGAG AGCCATCGGG AAGCATGGAC GAGCCTCCTG 
CTGCTACTTC TAACCAAAAC CCTCAAGATA AGCGATGAAA AGTTCAAAGC ACACGCGTCA 
ATGTACTACC CCTACCTGTG TGAAATTATG CAGTTTGACC TGATCCCTGA GCTCCGAGCA 
GTTCTGCGAA AGTTCTTCCT GCGGATAGGC CTGGTGTATA AGATATGGAT TCCAGAAGAG 
CCGTCGCAAG TGCCAGCAGC ACTGTCATCG ACCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgef2 ( XM_006500483.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500546.1 Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X1, mRNA.

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CloneID	OMu31077
Clone ID Related Accession (Same CDS sequence)	XM_006500483.3 , XM_006500483.4
Accession Version	XM_006500483.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500483.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAAA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCT 
GACAAGGAGG TGAAGCGGCC CCAGCACTCC CAGCTGCGTA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAACT AGAAAAGCAG AGGCTTGGCG CTGCGGCTCC ACCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTCCTT CCATTCGAGC TGGCCTGCCA ATCCAAGTCA 
CCTCGGGTGG TCAGCACATC CCTGGACTGC TTGCAGAAAC TCATCGCATA TGGACACATC 
ACTGGCAATG CCCCTGACAG TGGCGCTCCT GGGAAGCGGC TGATCGACAG GATCGTTGAA 
ACCATTTGCA ATTGTTTTCA GGGCCCTCAG ACAGATGAAG GGGTTCAGCT ACAAATCATT 
AAGGCTCTGC TGACTGCTGT GACATCCCCA CACATTGAAA TCCATGAGGG CACCATCCTG 
CAGACAGTGA GGACCTGCTA CAATATCTAT CTGGCCAGCA AAAACCTCAT CAATCAAACC 
ACCGCCAAGG CCACCCTTAC CCAAATGCTG AATGTCATCT TCACCCGCAT GGAAAACCAA 
GTGTTACAGG AGGCCAGAGA ATTGGAAAAA CCAATGCAGT CAAAACCTCA GTCCCCGGTG 
ATCCAGGCCA CAGCAGGCTC TCCCAAGTTC AGCCGCCTGA AGCAGAGCCA GGCCCAGAGC 
AAACCCACGA CTCCTGAGAA AGCAGAGCTG CCCAATGGGG ACCATGCCCA GAGTGGCCTA 
GGAAAAGTGA GCTTGGAAAA TGGAGAAGCT CCCCGAGAGA GAGGCTCGCC AGTCTCTGGG 
AGAGCCGAGC CTTCGAGGGG CACTGACAGT GGAGCTCAGG AGGTGGTGAA GGACATCTTA 
GAGGACGTGG TCACGTCTGC TGTGAAAGCA GCAGAAAAGC ATGGTCTGCC GGAGCCCGAC 
CGAGCCCTGG GTGCCCTAGA GTGCCAGGAA TGCGCTGTTC CTCCTGGAGT TGATGAAAAC 
TCACAGACCA ACGGCATAGC AGACGACAGG CAGTCGCTAT CCTCCGCAGA TAATCTGGAA 
CCAGATGTTC AAGGACACCA AGTGGCTGCT AGGTTCTCTC ACATTCTACA GAAGGATGCC 
TTCCTGGTGT TCCGCTCCCT GTGCAAGCTG TCCATGAAGC CCCTTGGGGA AGGGCCACCA 
GATCCAAAGT CCCACGAGCT TCGTTCCAAG GTGGTTTCCC TGCAGCTGCT CCTCTCTGTG 
CTGCAGAATG CTGGCCCAGT CTTCAGGAGC CACGAGATGT TCGTCACTGC CATTAAGCAG 
TACCTGTGTG TGGCCCTTTC CAAGAATGGC GTGTCCTCAG TGCCTGACGT CTTCGAACTC 
TCGCTTGCTA TTTTTCTTAC TCTTCTTTCA AACTTCAAGA TGCACTTGAA AATGCAGATA 
GAGGTCTTTT TCAAAGAGAT TTTCCTGAAC ATTTTAGAAA CATCCACAAG TTCTTTTGAG 
CACCGCTGGA TGGTCATTCA GACCCTGACG AGGATTTGTG CAGATGCCCA GTGCGTTGTG 
GATATTTATG TCAACTACGA CTGTGACTTA AATGCTGCTA ACATTTTCGA GCGACTAGTA 
AATGACTTAT CCAAAATTGC TCAGGGAAGA AGTGGACATG AGCTGGGGAT GACACCTCTA 
CAGGAGCTCA GTCTGAGGAA GAAAGGCCTG GAGTGTCTGG TGTCTATTCT CAAGTGCATG 
GTGGAGTGGA GCAAGGACCT GTATGTGAAT CCCAACCACC AGGCTACCCT GGGTCAGGAG 
AGGCTCCCAG ATCAGGAAAT GGGGGACGGA AAAGGCCTTG ACATGGCAAG ACGGTGCAGC 
GTGACATCCG TGGAGTCCAC AGTGTCCTCG GGGACCCAGA CAGCCATTCA GGATGACCCA 
GAGCAGTTTG AGGTCATCAA ACAACAGAAG GAAATCATTG AGCATGGCAT TGAGCTCTTC 
AATAAGAAGC CCAAGCGAGG TATCCAGTTT CTCCAAGAGC AAGGCATGCT GGGAGCAGCT 
GTAGAAGATA TTGCCCAATT TCTGCACCAG GAGGAGCGGC TGGATTCTAC CCAGGTTGGC 
GAGTTTCTGG GAGACAGCAC GAGGTTTAAC AAGGAGGTGA TGTATGCCTA TGTGGACCAA 
CTGGATTTCT GTGAAAAAGA GTTCGTCTCA GCCCTGAGGA CATTCCTGGA GGGCTTCCGC 
CTGCCTGGGG AAGCCCAGAA GATTGACCGG TTAATGGAGA AGTTTGCTGC ACGATACATA 
GAATGTAACC AAGGGCAAAC CCTGTTTGCT AGTGCGGACA CTGCGTATGT CCTGGCGTAC 
TCCATCATCA TGCTGACCAC AGACCTGCAC AGCCCACAGG TAAAGAATAA GATGACAAAA 
GAGCAGTATA TTAAAATGAA CCGGGGCATC AACGACAGCA AAGACCTGCC TGAGGAGTAC 
CTGTCCAGCA TCTATGACGA GATAGAGGGC AAGAAGATAG CGATGAAGGA GACAAAGGAG 
CACACAATTG CGACCAAGTC CACCAAGCAG AGTGTAGCTA GTGAAAAGCA GAGGCGGCTG 
CTGTACAATG TGGAGATGGA GCAGATGGCT AAAACAGCCA AAGCTCTGAT GGAGGCCGTG 
AGCCATGCCA AAGCCCCGTT TACCAGCGCC ACACACTTGG ACCATGTCCG GCCAATGTTC 
AAACTGGTGT GGACGCCGCT GCTGGCAGCC TATAGTATTG GCCTGCAGAA CTGTGATGAC 
ACGGAGGTGG CCTCCCTGTG TTTGGAAGGC ATCAGGTGTG CAGTCCGCAT TGCCTGCATC 
TTTGGAATGC AGCTGGAACG AGATGCCTAT GTTCAGGCCC TTGCTCGCTT TTCCCTGCTG 
ACAGCCAGCT CCAGCATCAC AGAAATGAAG CAGAAAAACA TCGACACCAT AAAGACACTC 
ATCACTGTGG CTCACACCGA TGGCAACTAC CTTGGCAACT CCTGGCATGA GATCTTGAAG 
TGCATCAGCC AGCTGGAGCT TGCTCAGCTG ATAGGAACTG GGGTGAAGAC TCGCTACCTC 
TCTGGCTCTG GGCGGGAACG AGAAGGGAGC CTGAAGGGCC ACAGTCTGGC AGGAGAAGAA 
TTCATGGGTC TTGGCCTCGG TAACTTGGTG AGTGGTGGTG TAGACAAAAG ACAGATGGCC 
AGCTTCCAGG AGTCGGTTGG TGAAACAAGC TCACAGAGTG TGGTTGTCGC AGTTGACAGA 
ATTTTTACTG GCTCTACCAG ACTGGATGGA AATGCAATAG TTGACTTTGT CCGTTGGCTG 
TGTGCTGTGT CCATGGATGA GCTGGCTTCT CCCCACCATC CTCGAATGTT CAGCCTGCAG 
AAGATTGTGG AAATATCATA CTACAACATG AACAGGATCC GGCTGCAGTG GTCTCGGATA 
TGGCATGTCA TTGGAGATCA CTTCAATAAG GTCGGCTGTA ACCCCAATGA AGATGTGGCC 
ATCTTCGCAG TTGACTCTCT GCGGCAGCTC TCCATGAAGT TCCTGGAGAA GGGAGAATTA 
GCCAACTTCC GATTTCAGAA GGATTTTCTG AGGCCATTTG AACATATTAT GAAGAAAAAC 
AGGTCCCCGA CCATCCGGGA CATGGTGATC CGATGCATCG CTCAGATGGT GAGCTCCCAG 
GCAGCCAACA TCCGCTCAGG CTGGAAGAAC ATCTTTGCTG TGTTCCACCA GGCTGCCTCT 
GACCACGATG GGAACATTGT GGAGCTGGCC TTCCAAACCA CAGGCCACAT CGTCTCAACC 
ATCTTCCAGC ACCACTTCCC TGCAGCCATC GACTCCTTCC AGGATGCTGT GAAGTGCTTG 
TCTGAGTTCG CCTGCAATGC AGCTTTCCCT GACACCAGCA TGGAGGCCAT CCGGCTCATC 
CGCTTCTGTG GGAAATACGT CTCAGAGAGG CCTCGGGTGC TGCAAGAGTA CACAAGTGAT 
GATATGAATG TGGCTCCTGG TGACAGGGTC TGGGTCAGAG GCTGGTTTCC CATCCTGTTT 
GAACTCTCCT GCATCATTAA CAGATGCAAG TTAGATGTAC GCACAAGGGG ACTCACGGTC 
ATGTTTGAGA TTATGAAGAG CTATGGCCAC ACCTTTGCAA AGCACTGGTG GCAGGACCTG 
TTCAGAATCG TGTTTCGAAT TTTTGACAAC ATGAAACTCC CTGAGCAACA GTCAGAGAAA 
TCAGAGTGGA TGACGACCAC GTGCAATCAT GCACTGTACG CTATTTGTGA CGTATTCACC 
CAGTTCTACG AAGCTTTGCA TGAAGTGCTT CTCTCTGATG TGTTTGCGCA GCTGCAGTGG 
TGCGTGAAAC AAGATAATGA GCAGTTGGCC CGATCAGGTA CGAACTGCTT AGAGAACCTA 
GTGATATCCA ACGGCGAGAA GTTCAGCCCT GCTGTCTGGG ATGAAACCTG CAATTGCATG 
TTGGATATTT TCAAAACCAC CATCCCACAT GTTTTGCTGA CGTGGAGACC TGCGGGGATG 
GAGGAGGAGG TGTCAGATAG ACATCTGGAT GTGGACCTAG ACCGCCAGTC TTTAAGCAGC 
ATAGACAGAA ACGCCTCCGA GAGAGGACAG AGCCAGCTGT CCAACCCCAC TGACGACAGC 
TGGAAGGGGG CACCGTATGC ACATCAGAAA CTGCTGGCCA GCCTCCTCAT CAAGTGTGTG 
GTTCAGCTGG AGCTGATCCA GACCATTGAC AACATCGTGT TCTACCCTGC CACCAGCAAG 
AAGGAGGATG CGGAGCACAT GGTTGCTGCC CAGCAAGACA CCCTCGATGC AGAGATCCAC 
ATCGAGACGG AGAATCAGGG CATGTACAAG TTCATGTCCT CCCAGCACCT CTTCAAGCTG 
CTGGACTGCC TGCAGGAGTC CCACTCCTTC TCCAAAGCCT TCAACTCCAA CTATGAGCAG 
CGGACTGTCC TCTGGCGAGC TGGCTTCAAG GGCAAGTCCA AACCCAATCT TCTCAAACAA 
GAAACCAGCA GCCTGGCCTG TTGTCTAAGG ATCCTGTTTA GAATGTACGT TGATGAGAAC 
CGCAGGGATT CCTGGGACGA AATACAGCAG CGACTTCTAA GAGTGTGCAG TGAAGCACTT 
GCCTATTTCA TTACTGTGAA CTCCGAGAGC CATCGGGAAG CATGGACGAG CCTCCTGCTG 
CTACTTCTAA CCAAAACCCT CAAGATAAGC GATGAAAAGT TCAAAGCACA CGCGTCAATG 
TACTACCCCT ACCTGTGTGA AATTATGCAG TTTGACCTGA TCCCTGAGCT CCGAGCAGTT 
CTGCGAAAGT TCTTCCTGCG GATAGGCCTG GTGTATAAGA TATGGATTCC AGAAGAGCCG 
TCGCAAGTGC CAGCAGCACT GTCATCGACC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500546.1
CDS	253..5628
Translation	MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAELEKQRLGAAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPMQSKPQSPVIQATAGSPKFSRLKQSQAQSKPTTPEKAELPNGDHAQSGLGKVSLENGEAPRERGSPVSGRAEPSRGTDSGAQEVVKDILEDVVTSAVKAAEKHGLPEPDRALGALECQECAVPPGVDENSQTNGIADDRQSLSSADNLEPDVQGHQVAARFSHILQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRSHEMFVTAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQATLGQERLPDQEMGDGKGLDMARRCSVTSVESTVSSGTQTAIQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGAAVEDIAQFLHQEERLDSTQVGEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYDEIEGKKIAMKETKEHTIATKSTKQSVASEKQRRLLYNVEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHSLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVSTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWWQDLFRIVFRIFDNMKLPEQQSEKSEWMTTTCNHALYAICDVFTQFYEALHEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPAVWDETCNCMLDIFKTTIPHVLLTWRPAGMEEEVSDRHLDVDLDRQSLSSIDRNASERGQSQLSNPTDDSWKGAPYAHQKLLASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDAEIHIETENQGMYKFMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWDEIQQRLLRVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKISDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGLVYKIWIPEEPSQVPAALSSTW

Target ORF information:

RefSeq Version	XM_006500483.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500483.3

ORF Insert Sequence:

ATGCAGGAAA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCT 
GACAAGGAGG TGAAGCGGCC CCAGCACTCC CAGCTGCGTA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAACT AGAAAAGCAG AGGCTTGGCG CTGCGGCTCC ACCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTCCTT CCATTCGAGC TGGCCTGCCA ATCCAAGTCA 
CCTCGGGTGG TCAGCACATC CCTGGACTGC TTGCAGAAAC TCATCGCATA TGGACACATC 
ACTGGCAATG CCCCTGACAG TGGCGCTCCT GGGAAGCGGC TGATCGACAG GATCGTTGAA 
ACCATTTGCA ATTGTTTTCA GGGCCCTCAG ACAGATGAAG GGGTTCAGCT ACAAATCATT 
AAGGCTCTGC TGACTGCTGT GACATCCCCA CACATTGAAA TCCATGAGGG CACCATCCTG 
CAGACAGTGA GGACCTGCTA CAATATCTAT CTGGCCAGCA AAAACCTCAT CAATCAAACC 
ACCGCCAAGG CCACCCTTAC CCAAATGCTG AATGTCATCT TCACCCGCAT GGAAAACCAA 
GTGTTACAGG AGGCCAGAGA ATTGGAAAAA CCAATGCAGT CAAAACCTCA GTCCCCGGTG 
ATCCAGGCCA CAGCAGGCTC TCCCAAGTTC AGCCGCCTGA AGCAGAGCCA GGCCCAGAGC 
AAACCCACGA CTCCTGAGAA AGCAGAGCTG CCCAATGGGG ACCATGCCCA GAGTGGCCTA 
GGAAAAGTGA GCTTGGAAAA TGGAGAAGCT CCCCGAGAGA GAGGCTCGCC AGTCTCTGGG 
AGAGCCGAGC CTTCGAGGGG CACTGACAGT GGAGCTCAGG AGGTGGTGAA GGACATCTTA 
GAGGACGTGG TCACGTCTGC TGTGAAAGCA GCAGAAAAGC ATGGTCTGCC GGAGCCCGAC 
CGAGCCCTGG GTGCCCTAGA GTGCCAGGAA TGCGCTGTTC CTCCTGGAGT TGATGAAAAC 
TCACAGACCA ACGGCATAGC AGACGACAGG CAGTCGCTAT CCTCCGCAGA TAATCTGGAA 
CCAGATGTTC AAGGACACCA AGTGGCTGCT AGGTTCTCTC ACATTCTACA GAAGGATGCC 
TTCCTGGTGT TCCGCTCCCT GTGCAAGCTG TCCATGAAGC CCCTTGGGGA AGGGCCACCA 
GATCCAAAGT CCCACGAGCT TCGTTCCAAG GTGGTTTCCC TGCAGCTGCT CCTCTCTGTG 
CTGCAGAATG CTGGCCCAGT CTTCAGGAGC CACGAGATGT TCGTCACTGC CATTAAGCAG 
TACCTGTGTG TGGCCCTTTC CAAGAATGGC GTGTCCTCAG TGCCTGACGT CTTCGAACTC 
TCGCTTGCTA TTTTTCTTAC TCTTCTTTCA AACTTCAAGA TGCACTTGAA AATGCAGATA 
GAGGTCTTTT TCAAAGAGAT TTTCCTGAAC ATTTTAGAAA CATCCACAAG TTCTTTTGAG 
CACCGCTGGA TGGTCATTCA GACCCTGACG AGGATTTGTG CAGATGCCCA GTGCGTTGTG 
GATATTTATG TCAACTACGA CTGTGACTTA AATGCTGCTA ACATTTTCGA GCGACTAGTA 
AATGACTTAT CCAAAATTGC TCAGGGAAGA AGTGGACATG AGCTGGGGAT GACACCTCTA 
CAGGAGCTCA GTCTGAGGAA GAAAGGCCTG GAGTGTCTGG TGTCTATTCT CAAGTGCATG 
GTGGAGTGGA GCAAGGACCT GTATGTGAAT CCCAACCACC AGGCTACCCT GGGTCAGGAG 
AGGCTCCCAG ATCAGGAAAT GGGGGACGGA AAAGGCCTTG ACATGGCAAG ACGGTGCAGC 
GTGACATCCG TGGAGTCCAC AGTGTCCTCG GGGACCCAGA CAGCCATTCA GGATGACCCA 
GAGCAGTTTG AGGTCATCAA ACAACAGAAG GAAATCATTG AGCATGGCAT TGAGCTCTTC 
AATAAGAAGC CCAAGCGAGG TATCCAGTTT CTCCAAGAGC AAGGCATGCT GGGAGCAGCT 
GTAGAAGATA TTGCCCAATT TCTGCACCAG GAGGAGCGGC TGGATTCTAC CCAGGTTGGC 
GAGTTTCTGG GAGACAGCAC GAGGTTTAAC AAGGAGGTGA TGTATGCCTA TGTGGACCAA 
CTGGATTTCT GTGAAAAAGA GTTCGTCTCA GCCCTGAGGA CATTCCTGGA GGGCTTCCGC 
CTGCCTGGGG AAGCCCAGAA GATTGACCGG TTAATGGAGA AGTTTGCTGC ACGATACATA 
GAATGTAACC AAGGGCAAAC CCTGTTTGCT AGTGCGGACA CTGCGTATGT CCTGGCGTAC 
TCCATCATCA TGCTGACCAC AGACCTGCAC AGCCCACAGG TAAAGAATAA GATGACAAAA 
GAGCAGTATA TTAAAATGAA CCGGGGCATC AACGACAGCA AAGACCTGCC TGAGGAGTAC 
CTGTCCAGCA TCTATGACGA GATAGAGGGC AAGAAGATAG CGATGAAGGA GACAAAGGAG 
CACACAATTG CGACCAAGTC CACCAAGCAG AGTGTAGCTA GTGAAAAGCA GAGGCGGCTG 
CTGTACAATG TGGAGATGGA GCAGATGGCT AAAACAGCCA AAGCTCTGAT GGAGGCCGTG 
AGCCATGCCA AAGCCCCGTT TACCAGCGCC ACACACTTGG ACCATGTCCG GCCAATGTTC 
AAACTGGTGT GGACGCCGCT GCTGGCAGCC TATAGTATTG GCCTGCAGAA CTGTGATGAC 
ACGGAGGTGG CCTCCCTGTG TTTGGAAGGC ATCAGGTGTG CAGTCCGCAT TGCCTGCATC 
TTTGGAATGC AGCTGGAACG AGATGCCTAT GTTCAGGCCC TTGCTCGCTT TTCCCTGCTG 
ACAGCCAGCT CCAGCATCAC AGAAATGAAG CAGAAAAACA TCGACACCAT AAAGACACTC 
ATCACTGTGG CTCACACCGA TGGCAACTAC CTTGGCAACT CCTGGCATGA GATCTTGAAG 
TGCATCAGCC AGCTGGAGCT TGCTCAGCTG ATAGGAACTG GGGTGAAGAC TCGCTACCTC 
TCTGGCTCTG GGCGGGAACG AGAAGGGAGC CTGAAGGGCC ACAGTCTGGC AGGAGAAGAA 
TTCATGGGTC TTGGCCTCGG TAACTTGGTG AGTGGTGGTG TAGACAAAAG ACAGATGGCC 
AGCTTCCAGG AGTCGGTTGG TGAAACAAGC TCACAGAGTG TGGTTGTCGC AGTTGACAGA 
ATTTTTACTG GCTCTACCAG ACTGGATGGA AATGCAATAG TTGACTTTGT CCGTTGGCTG 
TGTGCTGTGT CCATGGATGA GCTGGCTTCT CCCCACCATC CTCGAATGTT CAGCCTGCAG 
AAGATTGTGG AAATATCATA CTACAACATG AACAGGATCC GGCTGCAGTG GTCTCGGATA 
TGGCATGTCA TTGGAGATCA CTTCAATAAG GTCGGCTGTA ACCCCAATGA AGATGTGGCC 
ATCTTCGCAG TTGACTCTCT GCGGCAGCTC TCCATGAAGT TCCTGGAGAA GGGAGAATTA 
GCCAACTTCC GATTTCAGAA GGATTTTCTG AGGCCATTTG AACATATTAT GAAGAAAAAC 
AGGTCCCCGA CCATCCGGGA CATGGTGATC CGATGCATCG CTCAGATGGT GAGCTCCCAG 
GCAGCCAACA TCCGCTCAGG CTGGAAGAAC ATCTTTGCTG TGTTCCACCA GGCTGCCTCT 
GACCACGATG GGAACATTGT GGAGCTGGCC TTCCAAACCA CAGGCCACAT CGTCTCAACC 
ATCTTCCAGC ACCACTTCCC TGCAGCCATC GACTCCTTCC AGGATGCTGT GAAGTGCTTG 
TCTGAGTTCG CCTGCAATGC AGCTTTCCCT GACACCAGCA TGGAGGCCAT CCGGCTCATC 
CGCTTCTGTG GGAAATACGT CTCAGAGAGG CCTCGGGTGC TGCAAGAGTA CACAAGTGAT 
GATATGAATG TGGCTCCTGG TGACAGGGTC TGGGTCAGAG GCTGGTTTCC CATCCTGTTT 
GAACTCTCCT GCATCATTAA CAGATGCAAG TTAGATGTAC GCACAAGGGG ACTCACGGTC 
ATGTTTGAGA TTATGAAGAG CTATGGCCAC ACCTTTGCAA AGCACTGGTG GCAGGACCTG 
TTCAGAATCG TGTTTCGAAT TTTTGACAAC ATGAAACTCC CTGAGCAACA GTCAGAGAAA 
TCAGAGTGGA TGACGACCAC GTGCAATCAT GCACTGTACG CTATTTGTGA CGTATTCACC 
CAGTTCTACG AAGCTTTGCA TGAAGTGCTT CTCTCTGATG TGTTTGCGCA GCTGCAGTGG 
TGCGTGAAAC AAGATAATGA GCAGTTGGCC CGATCAGGTA CGAACTGCTT AGAGAACCTA 
GTGATATCCA ACGGCGAGAA GTTCAGCCCT GCTGTCTGGG ATGAAACCTG CAATTGCATG 
TTGGATATTT TCAAAACCAC CATCCCACAT GTTTTGCTGA CGTGGAGACC TGCGGGGATG 
GAGGAGGAGG TGTCAGATAG ACATCTGGAT GTGGACCTAG ACCGCCAGTC TTTAAGCAGC 
ATAGACAGAA ACGCCTCCGA GAGAGGACAG AGCCAGCTGT CCAACCCCAC TGACGACAGC 
TGGAAGGGGG CACCGTATGC ACATCAGAAA CTGCTGGCCA GCCTCCTCAT CAAGTGTGTG 
GTTCAGCTGG AGCTGATCCA GACCATTGAC AACATCGTGT TCTACCCTGC CACCAGCAAG 
AAGGAGGATG CGGAGCACAT GGTTGCTGCC CAGCAAGACA CCCTCGATGC AGAGATCCAC 
ATCGAGACGG AGAATCAGGG CATGTACAAG TTCATGTCCT CCCAGCACCT CTTCAAGCTG 
CTGGACTGCC TGCAGGAGTC CCACTCCTTC TCCAAAGCCT TCAACTCCAA CTATGAGCAG 
CGGACTGTCC TCTGGCGAGC TGGCTTCAAG GGCAAGTCCA AACCCAATCT TCTCAAACAA 
GAAACCAGCA GCCTGGCCTG TTGTCTAAGG ATCCTGTTTA GAATGTACGT TGATGAGAAC 
CGCAGGGATT CCTGGGACGA AATACAGCAG CGACTTCTAA GAGTGTGCAG TGAAGCACTT 
GCCTATTTCA TTACTGTGAA CTCCGAGAGC CATCGGGAAG CATGGACGAG CCTCCTGCTG 
CTACTTCTAA CCAAAACCCT CAAGATAAGC GATGAAAAGT TCAAAGCACA CGCGTCAATG 
TACTACCCCT ACCTGTGTGA AATTATGCAG TTTGACCTGA TCCCTGAGCT CCGAGCAGTT 
CTGCGAAAGT TCTTCCTGCG GATAGGCCTG GTGTATAAGA TATGGATTCC AGAAGAGCCG 
TCGCAAGTGC CAGCAGCACT GTCATCGACC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgef2 ( XM_006500484.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500547.1 Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X2, mRNA.

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CloneID	OMu31078
Clone ID Related Accession (Same CDS sequence)	XM_006500484.3 , XM_006500484.4
Accession Version	XM_006500484.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500484.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAAA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCT 
GACAAGGAGG TGAAGCGGCC CCAGCACTCC CAGCTGCGTA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAACT AGAAAAGCAG AGGCTTGGCG CTGCGGCTCC ACCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTCCTT CCATTCGAGC TGGCCTGCCA ATCCAAGTCA 
CCTCGGGTGG TCAGCACATC CCTGGACTGC TTGCAGAAAC TCATCGCATA TGGACACATC 
ACTGGCAATG CCCCTGACAG TGGCGCTCCT GGGAAGCGGC TGATCGACAG GATCGTTGAA 
ACCATTTGCA ATTGTTTTCA GGGCCCTCAG ACAGATGAAG GGGTTCAGCT ACAAATCATT 
AAGGCTCTGC TGACTGCTGT GACATCCCCA CACATTGAAA TCCATGAGGG CACCATCCTG 
CAGACAGTGA GGACCTGCTA CAATATCTAT CTGGCCAGCA AAAACCTCAT CAATCAAACC 
ACCGCCAAGG CCACCCTTAC CCAAATGCTG AATGTCATCT TCACCCGCAT GGAAAACCAA 
GTGTTACAGG AGGCCAGAGA ATTGGAAAAA CCAATGCAGT CAAAACCTCA GTCCCCGGTG 
ATCCAGGCCA CAGCAGGCTC TCCCAAGTTC AGCCGCCTGA AGCAGAGCCA GGCCCAGAGC 
AAACCCACGA CTCCTGAGAA AGCAGAGCTG CCCAATGGGG ACCATGCCCA GAGTGGCCTA 
GGAAAAGTGA GCTTGGAAAA TGGAGAAGCT CCCCGAGAGA GAGGCTCGCC AGTCTCTGGG 
AGAGCCGAGC CTTCGAGGGA AGCAGCAGAA AAGCATGGTC TGCCGGAGCC CGACCGAGCC 
CTGGGTGCCC TAGAGTGCCA GGAATGCGCT GTTCCTCCTG GAGTTGATGA AAACTCACAG 
ACCAACGGCA TAGCAGACGA CAGGCAGTCG CTATCCTCCG CAGATAATCT GGAACCAGAT 
GTTCAAGGAC ACCAAGTGGC TGCTAGGTTC TCTCACATTC TACAGAAGGA TGCCTTCCTG 
GTGTTCCGCT CCCTGTGCAA GCTGTCCATG AAGCCCCTTG GGGAAGGGCC ACCAGATCCA 
AAGTCCCACG AGCTTCGTTC CAAGGTGGTT TCCCTGCAGC TGCTCCTCTC TGTGCTGCAG 
AATGCTGGCC CAGTCTTCAG GAGCCACGAG ATGTTCGTCA CTGCCATTAA GCAGTACCTG 
TGTGTGGCCC TTTCCAAGAA TGGCGTGTCC TCAGTGCCTG ACGTCTTCGA ACTCTCGCTT 
GCTATTTTTC TTACTCTTCT TTCAAACTTC AAGATGCACT TGAAAATGCA GATAGAGGTC 
TTTTTCAAAG AGATTTTCCT GAACATTTTA GAAACATCCA CAAGTTCTTT TGAGCACCGC 
TGGATGGTCA TTCAGACCCT GACGAGGATT TGTGCAGATG CCCAGTGCGT TGTGGATATT 
TATGTCAACT ACGACTGTGA CTTAAATGCT GCTAACATTT TCGAGCGACT AGTAAATGAC 
TTATCCAAAA TTGCTCAGGG AAGAAGTGGA CATGAGCTGG GGATGACACC TCTACAGGAG 
CTCAGTCTGA GGAAGAAAGG CCTGGAGTGT CTGGTGTCTA TTCTCAAGTG CATGGTGGAG 
TGGAGCAAGG ACCTGTATGT GAATCCCAAC CACCAGGCTA CCCTGGGTCA GGAGAGGCTC 
CCAGATCAGG AAATGGGGGA CGGAAAAGGC CTTGACATGG CAAGACGGTG CAGCGTGACA 
TCCGTGGAGT CCACAGTGTC CTCGGGGACC CAGACAGCCA TTCAGGATGA CCCAGAGCAG 
TTTGAGGTCA TCAAACAACA GAAGGAAATC ATTGAGCATG GCATTGAGCT CTTCAATAAG 
AAGCCCAAGC GAGGTATCCA GTTTCTCCAA GAGCAAGGCA TGCTGGGAGC AGCTGTAGAA 
GATATTGCCC AATTTCTGCA CCAGGAGGAG CGGCTGGATT CTACCCAGGT TGGCGAGTTT 
CTGGGAGACA GCACGAGGTT TAACAAGGAG GTGATGTATG CCTATGTGGA CCAACTGGAT 
TTCTGTGAAA AAGAGTTCGT CTCAGCCCTG AGGACATTCC TGGAGGGCTT CCGCCTGCCT 
GGGGAAGCCC AGAAGATTGA CCGGTTAATG GAGAAGTTTG CTGCACGATA CATAGAATGT 
AACCAAGGGC AAACCCTGTT TGCTAGTGCG GACACTGCGT ATGTCCTGGC GTACTCCATC 
ATCATGCTGA CCACAGACCT GCACAGCCCA CAGGTAAAGA ATAAGATGAC AAAAGAGCAG 
TATATTAAAA TGAACCGGGG CATCAACGAC AGCAAAGACC TGCCTGAGGA GTACCTGTCC 
AGCATCTATG ACGAGATAGA GGGCAAGAAG ATAGCGATGA AGGAGACAAA GGAGCACACA 
ATTGCGACCA AGTCCACCAA GCAGAGTGTA GCTAGTGAAA AGCAGAGGCG GCTGCTGTAC 
AATGTGGAGA TGGAGCAGAT GGCTAAAACA GCCAAAGCTC TGATGGAGGC CGTGAGCCAT 
GCCAAAGCCC CGTTTACCAG CGCCACACAC TTGGACCATG TCCGGCCAAT GTTCAAACTG 
GTGTGGACGC CGCTGCTGGC AGCCTATAGT ATTGGCCTGC AGAACTGTGA TGACACGGAG 
GTGGCCTCCC TGTGTTTGGA AGGCATCAGG TGTGCAGTCC GCATTGCCTG CATCTTTGGA 
ATGCAGCTGG AACGAGATGC CTATGTTCAG GCCCTTGCTC GCTTTTCCCT GCTGACAGCC 
AGCTCCAGCA TCACAGAAAT GAAGCAGAAA AACATCGACA CCATAAAGAC ACTCATCACT 
GTGGCTCACA CCGATGGCAA CTACCTTGGC AACTCCTGGC ATGAGATCTT GAAGTGCATC 
AGCCAGCTGG AGCTTGCTCA GCTGATAGGA ACTGGGGTGA AGACTCGCTA CCTCTCTGGC 
TCTGGGCGGG AACGAGAAGG GAGCCTGAAG GGCCACAGTC TGGCAGGAGA AGAATTCATG 
GGTCTTGGCC TCGGTAACTT GGTGAGTGGT GGTGTAGACA AAAGACAGAT GGCCAGCTTC 
CAGGAGTCGG TTGGTGAAAC AAGCTCACAG AGTGTGGTTG TCGCAGTTGA CAGAATTTTT 
ACTGGCTCTA CCAGACTGGA TGGAAATGCA ATAGTTGACT TTGTCCGTTG GCTGTGTGCT 
GTGTCCATGG ATGAGCTGGC TTCTCCCCAC CATCCTCGAA TGTTCAGCCT GCAGAAGATT 
GTGGAAATAT CATACTACAA CATGAACAGG ATCCGGCTGC AGTGGTCTCG GATATGGCAT 
GTCATTGGAG ATCACTTCAA TAAGGTCGGC TGTAACCCCA ATGAAGATGT GGCCATCTTC 
GCAGTTGACT CTCTGCGGCA GCTCTCCATG AAGTTCCTGG AGAAGGGAGA ATTAGCCAAC 
TTCCGATTTC AGAAGGATTT TCTGAGGCCA TTTGAACATA TTATGAAGAA AAACAGGTCC 
CCGACCATCC GGGACATGGT GATCCGATGC ATCGCTCAGA TGGTGAGCTC CCAGGCAGCC 
AACATCCGCT CAGGCTGGAA GAACATCTTT GCTGTGTTCC ACCAGGCTGC CTCTGACCAC 
GATGGGAACA TTGTGGAGCT GGCCTTCCAA ACCACAGGCC ACATCGTCTC AACCATCTTC 
CAGCACCACT TCCCTGCAGC CATCGACTCC TTCCAGGATG CTGTGAAGTG CTTGTCTGAG 
TTCGCCTGCA ATGCAGCTTT CCCTGACACC AGCATGGAGG CCATCCGGCT CATCCGCTTC 
TGTGGGAAAT ACGTCTCAGA GAGGCCTCGG GTGCTGCAAG AGTACACAAG TGATGATATG 
AATGTGGCTC CTGGTGACAG GGTCTGGGTC AGAGGCTGGT TTCCCATCCT GTTTGAACTC 
TCCTGCATCA TTAACAGATG CAAGTTAGAT GTACGCACAA GGGGACTCAC GGTCATGTTT 
GAGATTATGA AGAGCTATGG CCACACCTTT GCAAAGCACT GGTGGCAGGA CCTGTTCAGA 
ATCGTGTTTC GAATTTTTGA CAACATGAAA CTCCCTGAGC AACAGTCAGA GAAATCAGAG 
TGGATGACGA CCACGTGCAA TCATGCACTG TACGCTATTT GTGACGTATT CACCCAGTTC 
TACGAAGCTT TGCATGAAGT GCTTCTCTCT GATGTGTTTG CGCAGCTGCA GTGGTGCGTG 
AAACAAGATA ATGAGCAGTT GGCCCGATCA GGTACGAACT GCTTAGAGAA CCTAGTGATA 
TCCAACGGCG AGAAGTTCAG CCCTGCTGTC TGGGATGAAA CCTGCAATTG CATGTTGGAT 
ATTTTCAAAA CCACCATCCC ACATGTTTTG CTGACGTGGA GACCTGCGGG GATGGAGGAG 
GAGGTGTCAG ATAGACATCT GGATGTGGAC CTAGACCGCC AGTCTTTAAG CAGCATAGAC 
AGAAACGCCT CCGAGAGAGG ACAGAGCCAG CTGTCCAACC CCACTGACGA CAGCTGGAAG 
GGGGCACCGT ATGCACATCA GAAACTGCTG GCCAGCCTCC TCATCAAGTG TGTGGTTCAG 
CTGGAGCTGA TCCAGACCAT TGACAACATC GTGTTCTACC CTGCCACCAG CAAGAAGGAG 
GATGCGGAGC ACATGGTTGC TGCCCAGCAA GACACCCTCG ATGCAGAGAT CCACATCGAG 
ACGGAGAATC AGGGCATGTA CAAGTTCATG TCCTCCCAGC ACCTCTTCAA GCTGCTGGAC 
TGCCTGCAGG AGTCCCACTC CTTCTCCAAA GCCTTCAACT CCAACTATGA GCAGCGGACT 
GTCCTCTGGC GAGCTGGCTT CAAGGGCAAG TCCAAACCCA ATCTTCTCAA ACAAGAAACC 
AGCAGCCTGG CCTGTTGTCT AAGGATCCTG TTTAGAATGT ACGTTGATGA GAACCGCAGG 
GATTCCTGGG ACGAAATACA GCAGCGACTT CTAAGAGTGT GCAGTGAAGC ACTTGCCTAT 
TTCATTACTG TGAACTCCGA GAGCCATCGG GAAGCATGGA CGAGCCTCCT GCTGCTACTT 
CTAACCAAAA CCCTCAAGAT AAGCGATGAA AAGTTCAAAG CACACGCGTC AATGTACTAC 
CCCTACCTGT GTGAAATTAT GCAGTTTGAC CTGATCCCTG AGCTCCGAGC AGTTCTGCGA 
AAGTTCTTCC TGCGGATAGG CCTGGTGTAT AAGATATGGA TTCCAGAAGA GCCGTCGCAA 
GTGCCAGCAG CACTGTCATC GACCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500547.1
CDS	253..5562
Translation	MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAELEKQRLGAAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPMQSKPQSPVIQATAGSPKFSRLKQSQAQSKPTTPEKAELPNGDHAQSGLGKVSLENGEAPRERGSPVSGRAEPSREAAEKHGLPEPDRALGALECQECAVPPGVDENSQTNGIADDRQSLSSADNLEPDVQGHQVAARFSHILQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRSHEMFVTAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQATLGQERLPDQEMGDGKGLDMARRCSVTSVESTVSSGTQTAIQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGAAVEDIAQFLHQEERLDSTQVGEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYDEIEGKKIAMKETKEHTIATKSTKQSVASEKQRRLLYNVEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHSLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVSTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWWQDLFRIVFRIFDNMKLPEQQSEKSEWMTTTCNHALYAICDVFTQFYEALHEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPAVWDETCNCMLDIFKTTIPHVLLTWRPAGMEEEVSDRHLDVDLDRQSLSSIDRNASERGQSQLSNPTDDSWKGAPYAHQKLLASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDAEIHIETENQGMYKFMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWDEIQQRLLRVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKISDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGLVYKIWIPEEPSQVPAALSSTW

Target ORF information:

RefSeq Version	XM_006500484.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500484.3

ORF Insert Sequence:

ATGCAGGAAA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCT 
GACAAGGAGG TGAAGCGGCC CCAGCACTCC CAGCTGCGTA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAACT AGAAAAGCAG AGGCTTGGCG CTGCGGCTCC ACCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTCCTT CCATTCGAGC TGGCCTGCCA ATCCAAGTCA 
CCTCGGGTGG TCAGCACATC CCTGGACTGC TTGCAGAAAC TCATCGCATA TGGACACATC 
ACTGGCAATG CCCCTGACAG TGGCGCTCCT GGGAAGCGGC TGATCGACAG GATCGTTGAA 
ACCATTTGCA ATTGTTTTCA GGGCCCTCAG ACAGATGAAG GGGTTCAGCT ACAAATCATT 
AAGGCTCTGC TGACTGCTGT GACATCCCCA CACATTGAAA TCCATGAGGG CACCATCCTG 
CAGACAGTGA GGACCTGCTA CAATATCTAT CTGGCCAGCA AAAACCTCAT CAATCAAACC 
ACCGCCAAGG CCACCCTTAC CCAAATGCTG AATGTCATCT TCACCCGCAT GGAAAACCAA 
GTGTTACAGG AGGCCAGAGA ATTGGAAAAA CCAATGCAGT CAAAACCTCA GTCCCCGGTG 
ATCCAGGCCA CAGCAGGCTC TCCCAAGTTC AGCCGCCTGA AGCAGAGCCA GGCCCAGAGC 
AAACCCACGA CTCCTGAGAA AGCAGAGCTG CCCAATGGGG ACCATGCCCA GAGTGGCCTA 
GGAAAAGTGA GCTTGGAAAA TGGAGAAGCT CCCCGAGAGA GAGGCTCGCC AGTCTCTGGG 
AGAGCCGAGC CTTCGAGGGA AGCAGCAGAA AAGCATGGTC TGCCGGAGCC CGACCGAGCC 
CTGGGTGCCC TAGAGTGCCA GGAATGCGCT GTTCCTCCTG GAGTTGATGA AAACTCACAG 
ACCAACGGCA TAGCAGACGA CAGGCAGTCG CTATCCTCCG CAGATAATCT GGAACCAGAT 
GTTCAAGGAC ACCAAGTGGC TGCTAGGTTC TCTCACATTC TACAGAAGGA TGCCTTCCTG 
GTGTTCCGCT CCCTGTGCAA GCTGTCCATG AAGCCCCTTG GGGAAGGGCC ACCAGATCCA 
AAGTCCCACG AGCTTCGTTC CAAGGTGGTT TCCCTGCAGC TGCTCCTCTC TGTGCTGCAG 
AATGCTGGCC CAGTCTTCAG GAGCCACGAG ATGTTCGTCA CTGCCATTAA GCAGTACCTG 
TGTGTGGCCC TTTCCAAGAA TGGCGTGTCC TCAGTGCCTG ACGTCTTCGA ACTCTCGCTT 
GCTATTTTTC TTACTCTTCT TTCAAACTTC AAGATGCACT TGAAAATGCA GATAGAGGTC 
TTTTTCAAAG AGATTTTCCT GAACATTTTA GAAACATCCA CAAGTTCTTT TGAGCACCGC 
TGGATGGTCA TTCAGACCCT GACGAGGATT TGTGCAGATG CCCAGTGCGT TGTGGATATT 
TATGTCAACT ACGACTGTGA CTTAAATGCT GCTAACATTT TCGAGCGACT AGTAAATGAC 
TTATCCAAAA TTGCTCAGGG AAGAAGTGGA CATGAGCTGG GGATGACACC TCTACAGGAG 
CTCAGTCTGA GGAAGAAAGG CCTGGAGTGT CTGGTGTCTA TTCTCAAGTG CATGGTGGAG 
TGGAGCAAGG ACCTGTATGT GAATCCCAAC CACCAGGCTA CCCTGGGTCA GGAGAGGCTC 
CCAGATCAGG AAATGGGGGA CGGAAAAGGC CTTGACATGG CAAGACGGTG CAGCGTGACA 
TCCGTGGAGT CCACAGTGTC CTCGGGGACC CAGACAGCCA TTCAGGATGA CCCAGAGCAG 
TTTGAGGTCA TCAAACAACA GAAGGAAATC ATTGAGCATG GCATTGAGCT CTTCAATAAG 
AAGCCCAAGC GAGGTATCCA GTTTCTCCAA GAGCAAGGCA TGCTGGGAGC AGCTGTAGAA 
GATATTGCCC AATTTCTGCA CCAGGAGGAG CGGCTGGATT CTACCCAGGT TGGCGAGTTT 
CTGGGAGACA GCACGAGGTT TAACAAGGAG GTGATGTATG CCTATGTGGA CCAACTGGAT 
TTCTGTGAAA AAGAGTTCGT CTCAGCCCTG AGGACATTCC TGGAGGGCTT CCGCCTGCCT 
GGGGAAGCCC AGAAGATTGA CCGGTTAATG GAGAAGTTTG CTGCACGATA CATAGAATGT 
AACCAAGGGC AAACCCTGTT TGCTAGTGCG GACACTGCGT ATGTCCTGGC GTACTCCATC 
ATCATGCTGA CCACAGACCT GCACAGCCCA CAGGTAAAGA ATAAGATGAC AAAAGAGCAG 
TATATTAAAA TGAACCGGGG CATCAACGAC AGCAAAGACC TGCCTGAGGA GTACCTGTCC 
AGCATCTATG ACGAGATAGA GGGCAAGAAG ATAGCGATGA AGGAGACAAA GGAGCACACA 
ATTGCGACCA AGTCCACCAA GCAGAGTGTA GCTAGTGAAA AGCAGAGGCG GCTGCTGTAC 
AATGTGGAGA TGGAGCAGAT GGCTAAAACA GCCAAAGCTC TGATGGAGGC CGTGAGCCAT 
GCCAAAGCCC CGTTTACCAG CGCCACACAC TTGGACCATG TCCGGCCAAT GTTCAAACTG 
GTGTGGACGC CGCTGCTGGC AGCCTATAGT ATTGGCCTGC AGAACTGTGA TGACACGGAG 
GTGGCCTCCC TGTGTTTGGA AGGCATCAGG TGTGCAGTCC GCATTGCCTG CATCTTTGGA 
ATGCAGCTGG AACGAGATGC CTATGTTCAG GCCCTTGCTC GCTTTTCCCT GCTGACAGCC 
AGCTCCAGCA TCACAGAAAT GAAGCAGAAA AACATCGACA CCATAAAGAC ACTCATCACT 
GTGGCTCACA CCGATGGCAA CTACCTTGGC AACTCCTGGC ATGAGATCTT GAAGTGCATC 
AGCCAGCTGG AGCTTGCTCA GCTGATAGGA ACTGGGGTGA AGACTCGCTA CCTCTCTGGC 
TCTGGGCGGG AACGAGAAGG GAGCCTGAAG GGCCACAGTC TGGCAGGAGA AGAATTCATG 
GGTCTTGGCC TCGGTAACTT GGTGAGTGGT GGTGTAGACA AAAGACAGAT GGCCAGCTTC 
CAGGAGTCGG TTGGTGAAAC AAGCTCACAG AGTGTGGTTG TCGCAGTTGA CAGAATTTTT 
ACTGGCTCTA CCAGACTGGA TGGAAATGCA ATAGTTGACT TTGTCCGTTG GCTGTGTGCT 
GTGTCCATGG ATGAGCTGGC TTCTCCCCAC CATCCTCGAA TGTTCAGCCT GCAGAAGATT 
GTGGAAATAT CATACTACAA CATGAACAGG ATCCGGCTGC AGTGGTCTCG GATATGGCAT 
GTCATTGGAG ATCACTTCAA TAAGGTCGGC TGTAACCCCA ATGAAGATGT GGCCATCTTC 
GCAGTTGACT CTCTGCGGCA GCTCTCCATG AAGTTCCTGG AGAAGGGAGA ATTAGCCAAC 
TTCCGATTTC AGAAGGATTT TCTGAGGCCA TTTGAACATA TTATGAAGAA AAACAGGTCC 
CCGACCATCC GGGACATGGT GATCCGATGC ATCGCTCAGA TGGTGAGCTC CCAGGCAGCC 
AACATCCGCT CAGGCTGGAA GAACATCTTT GCTGTGTTCC ACCAGGCTGC CTCTGACCAC 
GATGGGAACA TTGTGGAGCT GGCCTTCCAA ACCACAGGCC ACATCGTCTC AACCATCTTC 
CAGCACCACT TCCCTGCAGC CATCGACTCC TTCCAGGATG CTGTGAAGTG CTTGTCTGAG 
TTCGCCTGCA ATGCAGCTTT CCCTGACACC AGCATGGAGG CCATCCGGCT CATCCGCTTC 
TGTGGGAAAT ACGTCTCAGA GAGGCCTCGG GTGCTGCAAG AGTACACAAG TGATGATATG 
AATGTGGCTC CTGGTGACAG GGTCTGGGTC AGAGGCTGGT TTCCCATCCT GTTTGAACTC 
TCCTGCATCA TTAACAGATG CAAGTTAGAT GTACGCACAA GGGGACTCAC GGTCATGTTT 
GAGATTATGA AGAGCTATGG CCACACCTTT GCAAAGCACT GGTGGCAGGA CCTGTTCAGA 
ATCGTGTTTC GAATTTTTGA CAACATGAAA CTCCCTGAGC AACAGTCAGA GAAATCAGAG 
TGGATGACGA CCACGTGCAA TCATGCACTG TACGCTATTT GTGACGTATT CACCCAGTTC 
TACGAAGCTT TGCATGAAGT GCTTCTCTCT GATGTGTTTG CGCAGCTGCA GTGGTGCGTG 
AAACAAGATA ATGAGCAGTT GGCCCGATCA GGTACGAACT GCTTAGAGAA CCTAGTGATA 
TCCAACGGCG AGAAGTTCAG CCCTGCTGTC TGGGATGAAA CCTGCAATTG CATGTTGGAT 
ATTTTCAAAA CCACCATCCC ACATGTTTTG CTGACGTGGA GACCTGCGGG GATGGAGGAG 
GAGGTGTCAG ATAGACATCT GGATGTGGAC CTAGACCGCC AGTCTTTAAG CAGCATAGAC 
AGAAACGCCT CCGAGAGAGG ACAGAGCCAG CTGTCCAACC CCACTGACGA CAGCTGGAAG 
GGGGCACCGT ATGCACATCA GAAACTGCTG GCCAGCCTCC TCATCAAGTG TGTGGTTCAG 
CTGGAGCTGA TCCAGACCAT TGACAACATC GTGTTCTACC CTGCCACCAG CAAGAAGGAG 
GATGCGGAGC ACATGGTTGC TGCCCAGCAA GACACCCTCG ATGCAGAGAT CCACATCGAG 
ACGGAGAATC AGGGCATGTA CAAGTTCATG TCCTCCCAGC ACCTCTTCAA GCTGCTGGAC 
TGCCTGCAGG AGTCCCACTC CTTCTCCAAA GCCTTCAACT CCAACTATGA GCAGCGGACT 
GTCCTCTGGC GAGCTGGCTT CAAGGGCAAG TCCAAACCCA ATCTTCTCAA ACAAGAAACC 
AGCAGCCTGG CCTGTTGTCT AAGGATCCTG TTTAGAATGT ACGTTGATGA GAACCGCAGG 
GATTCCTGGG ACGAAATACA GCAGCGACTT CTAAGAGTGT GCAGTGAAGC ACTTGCCTAT 
TTCATTACTG TGAACTCCGA GAGCCATCGG GAAGCATGGA CGAGCCTCCT GCTGCTACTT 
CTAACCAAAA CCCTCAAGAT AAGCGATGAA AAGTTCAAAG CACACGCGTC AATGTACTAC 
CCCTACCTGT GTGAAATTAT GCAGTTTGAC CTGATCCCTG AGCTCCGAGC AGTTCTGCGA 
AAGTTCTTCC TGCGGATAGG CCTGGTGTAT AAGATATGGA TTCCAGAAGA GCCGTCGCAA 
GTGCCAGCAG CACTGTCATC GACCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgef2 ( XM_011239875.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238177.1 Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X3, mRNA.

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CloneID	OMu71969
Clone ID Related Accession (Same CDS sequence)	XM_011239875.1
Accession Version	XM_011239875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAATG TCATCTTCAC CCGCATGGAA AACCAAGTGT TACAGGAGGC CAGAGAATTG 
GAAAAACCAA TGCAGTCAAA ACCTCAGTCC CCGGTGATCC AGGCCACAGC AGGCTCTCCC 
AAGTTCAGCC GCCTGAAGCA GAGCCAGGCC CAGAGCAAAC CCACGACTCC TGAGAAAGCA 
GAGCTGCCCA ATGGGGACCA TGCCCAGAGT GGCCTAGGAA AAGTGAGCTT GGAAAATGGA 
GAAGCTCCCC GAGAGAGAGG CTCGCCAGTC TCTGGGAGAG CCGAGCCTTC GAGGGAAGCA 
GCAGAAAAGC ATGGTCTGCC GGAGCCCGAC CGAGCCCTGG GTGCCCTAGA GTGCCAGGAA 
TGCGCTGTTC CTCCTGGAGT TGATGAAAAC TCACAGACCA ACGGCATAGC AGACGACAGG 
CAGTCGCTAT CCTCCGCAGA TAATCTGGAA CCAGATGTTC AAGGACACCA AGTGGCTGCT 
AGGTTCTCTC ACATTCTACA GAAGGATGCC TTCCTGGTGT TCCGCTCCCT GTGCAAGCTG 
TCCATGAAGC CCCTTGGGGA AGGGCCACCA GATCCAAAGT CCCACGAGCT TCGTTCCAAG 
GTGGTTTCCC TGCAGCTGCT CCTCTCTGTG CTGCAGAATG CTGGCCCAGT CTTCAGGAGC 
CACGAGATGT TCGTCACTGC CATTAAGCAG TACCTGTGTG TGGCCCTTTC CAAGAATGGC 
GTGTCCTCAG TGCCTGACGT CTTCGAACTC TCGCTTGCTA TTTTTCTTAC TCTTCTTTCA 
AACTTCAAGA TGCACTTGAA AATGCAGATA GAGGTCTTTT TCAAAGAGAT TTTCCTGAAC 
ATTTTAGAAA CATCCACAAG TTCTTTTGAG CACCGCTGGA TGGTCATTCA GACCCTGACG 
AGGATTTGTG CAGATGCCCA GTGCGTTGTG GATATTTATG TCAACTACGA CTGTGACTTA 
AATGCTGCTA ACATTTTCGA GCGACTAGTA AATGACTTAT CCAAAATTGC TCAGGGAAGA 
AGTGGACATG AGCTGGGGAT GACACCTCTA CAGGAGCTCA GTCTGAGGAA GAAAGGCCTG 
GAGTGTCTGG TGTCTATTCT CAAGTGCATG GTGGAGTGGA GCAAGGACCT GTATGTGAAT 
CCCAACCACC AGGCTACCCT GGGTCAGGAG AGGCTCCCAG ATCAGGAAAT GGGGGACGGA 
AAAGGCCTTG ACATGGCAAG ACGGTGCAGC GTGACATCCG TGGAGTCCAC AGTGTCCTCG 
GGGACCCAGA CAGCCATTCA GGATGACCCA GAGCAGTTTG AGGTCATCAA ACAACAGAAG 
GAAATCATTG AGCATGGCAT TGAGCTCTTC AATAAGAAGC CCAAGCGAGG TATCCAGTTT 
CTCCAAGAGC AAGGCATGCT GGGAGCAGCT GTAGAAGATA TTGCCCAATT TCTGCACCAG 
GAGGAGCGGC TGGATTCTAC CCAGGTTGGC GAGTTTCTGG GAGACAGCAC GAGGTTTAAC 
AAGGAGGTGA TGTATGCCTA TGTGGACCAA CTGGATTTCT GTGAAAAAGA GTTCGTCTCA 
GCCCTGAGGA CATTCCTGGA GGGCTTCCGC CTGCCTGGGG AAGCCCAGAA GATTGACCGG 
TTAATGGAGA AGTTTGCTGC ACGATACATA GAATGTAACC AAGGGCAAAC CCTGTTTGCT 
AGTGCGGACA CTGCGTATGT CCTGGCGTAC TCCATCATCA TGCTGACCAC AGACCTGCAC 
AGCCCACAGG TAAAGAATAA GATGACAAAA GAGCAGTATA TTAAAATGAA CCGGGGCATC 
AACGACAGCA AAGACCTGCC TGAGGAGTAC CTGTCCAGCA TCTATGACGA GATAGAGGGC 
AAGAAGATAG CGATGAAGGA GACAAAGGAG CACACAATTG CGACCAAGTC CACCAAGCAG 
AGTGTAGCTA GTGAAAAGCA GAGGCGGCTG CTGTACAATG TGGAGATGGA GCAGATGGCT 
AAAACAGCCA AAGCTCTGAT GGAGGCCGTG AGCCATGCCA AAGCCCCGTT TACCAGCGCC 
ACACACTTGG ACCATGTCCG GCCAATGTTC AAACTGGTGT GGACGCCGCT GCTGGCAGCC 
TATAGTATTG GCCTGCAGAA CTGTGATGAC ACGGAGGTGG CCTCCCTGTG TTTGGAAGGC 
ATCAGGTGTG CAGTCCGCAT TGCCTGCATC TTTGGAATGC AGCTGGAACG AGATGCCTAT 
GTTCAGGCCC TTGCTCGCTT TTCCCTGCTG ACAGCCAGCT CCAGCATCAC AGAAATGAAG 
CAGAAAAACA TCGACACCAT AAAGACACTC ATCACTGTGG CTCACACCGA TGGCAACTAC 
CTTGGCAACT CCTGGCATGA GATCTTGAAG TGCATCAGCC AGCTGGAGCT TGCTCAGCTG 
ATAGGAACTG GGGTGAAGAC TCGCTACCTC TCTGGCTCTG GGCGGGAACG AGAAGGGAGC 
CTGAAGGGCC ACAGTCTGGC AGGAGAAGAA TTCATGGGTC TTGGCCTCGG TAACTTGGTG 
AGTGGTGGTG TAGACAAAAG ACAGATGGCC AGCTTCCAGG AGTCGGTTGG TGAAACAAGC 
TCACAGAGTG TGGTTGTCGC AGTTGACAGA ATTTTTACTG GCTCTACCAG ACTGGATGGA 
AATGCAATAG TTGACTTTGT CCGTTGGCTG TGTGCTGTGT CCATGGATGA GCTGGCTTCT 
CCCCACCATC CTCGAATGTT CAGCCTGCAG AAGATTGTGG AAATATCATA CTACAACATG 
AACAGGATCC GGCTGCAGTG GTCTCGGATA TGGCATGTCA TTGGAGATCA CTTCAATAAG 
GTCGGCTGTA ACCCCAATGA AGATGTGGCC ATCTTCGCAG TTGACTCTCT GCGGCAGCTC 
TCCATGAAGT TCCTGGAGAA GGGAGAATTA GCCAACTTCC GATTTCAGAA GGATTTTCTG 
AGGCCATTTG AACATATTAT GAAGAAAAAC AGGTCCCCGA CCATCCGGGA CATGGTGATC 
CGATGCATCG CTCAGATGGT GAGCTCCCAG GCAGCCAACA TCCGCTCAGG CTGGAAGAAC 
ATCTTTGCTG TGTTCCACCA GGCTGCCTCT GACCACGATG GGAACATTGT GGAGCTGGCC 
TTCCAAACCA CAGGCCACAT CGTCTCAACC ATCTTCCAGC ACCACTTCCC TGCAGCCATC 
GACTCCTTCC AGGATGCTGT GAAGTGCTTG TCTGAGTTCG CCTGCAATGC AGCTTTCCCT 
GACACCAGCA TGGAGGCCAT CCGGCTCATC CGCTTCTGTG GGAAATACGT CTCAGAGAGG 
CCTCGGGTGC TGCAAGAGTA CACAAGTGAT GATATGAATG TGGCTCCTGG TGACAGGGTC 
TGGGTCAGAG GCTGGTTTCC CATCCTGTTT GAACTCTCCT GCATCATTAA CAGATGCAAG 
TTAGATGTAC GCACAAGGGG ACTCACGGTC ATGTTTGAGA TTATGAAGAG CTATGGCCAC 
ACCTTTGCAA AGCACTGGTG GCAGGACCTG TTCAGAATCG TGTTTCGAAT TTTTGACAAC 
ATGAAACTCC CTGAGCAACA GTCAGAGAAA TCAGAGTGGA TGACGACCAC GTGCAATCAT 
GCACTGTACG CTATTTGTGA CGTATTCACC CAGTTCTACG AAGCTTTGCA TGAAGTGCTT 
CTCTCTGATG TGTTTGCGCA GCTGCAGTGG TGCGTGAAAC AAGATAATGA GCAGTTGGCC 
CGATCAGGTA CGAACTGCTT AGAGAACCTA GTGATATCCA ACGGCGAGAA GTTCAGCCCT 
GCTGTCTGGG ATGAAACCTG CAATTGCATG TTGGATATTT TCAAAACCAC CATCCCACAT 
GTTTTGCTGA CGTGGAGACC TGCGGGGATG GAGGAGGAGG TGTCAGATAG ACATCTGGAT 
GTGGACCTAG ACCGCCAGTC TTTAAGCAGC ATAGACAGAA ACGCCTCCGA GAGAGGACAG 
AGCCAGCTGT CCAACCCCAC TGACGACAGC TGGAAGGGGG CACCGTATGC ACATCAGAAA 
CTGCTGGCCA GCCTCCTCAT CAAGTGTGTG GTTCAGCTGG AGCTGATCCA GACCATTGAC 
AACATCGTGT TCTACCCTGC CACCAGCAAG AAGGAGGATG CGGAGCACAT GGTTGCTGCC 
CAGCAAGACA CCCTCGATGC AGAGATCCAC ATCGAGACGG AGAATCAGGG CATGTACAAG 
TTCATGTCCT CCCAGCACCT CTTCAAGCTG CTGGACTGCC TGCAGGAGTC CCACTCCTTC 
TCCAAAGCCT TCAACTCCAA CTATGAGCAG CGGACTGTCC TCTGGCGAGC TGGCTTCAAG 
GGCAAGTCCA AACCCAATCT TCTCAAACAA GAAACCAGCA GCCTGGCCTG TTGTCTAAGG 
ATCCTGTTTA GAATGTACGT TGATGAGAAC CGCAGGGATT CCTGGGACGA AATACAGCAG 
CGACTTCTAA GAGTGTGCAG TGAAGCACTT GCCTATTTCA TTACTGTGAA CTCCGAGAGC 
CATCGGGAAG CATGGACGAG CCTCCTGCTG CTACTTCTAA CCAAAACCCT CAAGATAAGC 
GATGAAAAGT TCAAAGCACA CGCGTCAATG TACTACCCCT ACCTGTGTGA AATTATGCAG 
TTTGACCTGA TCCCTGAGCT CCGAGCAGTT CTGCGAAAGT TCTTCCTGCG GATAGGCCTG 
GTGTATAAGA TATGGATTCC AGAAGAGCCG TCGCAAGTGC CAGCAGCACT GTCATCGACC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238177.1
CDS	372..5117
Translation	MLNVIFTRMENQVLQEARELEKPMQSKPQSPVIQATAGSPKFSRLKQSQAQSKPTTPEKAELPNGDHAQSGLGKVSLENGEAPRERGSPVSGRAEPSREAAEKHGLPEPDRALGALECQECAVPPGVDENSQTNGIADDRQSLSSADNLEPDVQGHQVAARFSHILQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRSHEMFVTAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQATLGQERLPDQEMGDGKGLDMARRCSVTSVESTVSSGTQTAIQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGAAVEDIAQFLHQEERLDSTQVGEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYDEIEGKKIAMKETKEHTIATKSTKQSVASEKQRRLLYNVEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHSLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVSTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWWQDLFRIVFRIFDNMKLPEQQSEKSEWMTTTCNHALYAICDVFTQFYEALHEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPAVWDETCNCMLDIFKTTIPHVLLTWRPAGMEEEVSDRHLDVDLDRQSLSSIDRNASERGQSQLSNPTDDSWKGAPYAHQKLLASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDAEIHIETENQGMYKFMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWDEIQQRLLRVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKISDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGLVYKIWIPEEPSQVPAALSSTW

Target ORF information:

RefSeq Version	XM_011239875.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239875.1

ORF Insert Sequence:

ATGCTGAATG TCATCTTCAC CCGCATGGAA AACCAAGTGT TACAGGAGGC CAGAGAATTG 
GAAAAACCAA TGCAGTCAAA ACCTCAGTCC CCGGTGATCC AGGCCACAGC AGGCTCTCCC 
AAGTTCAGCC GCCTGAAGCA GAGCCAGGCC CAGAGCAAAC CCACGACTCC TGAGAAAGCA 
GAGCTGCCCA ATGGGGACCA TGCCCAGAGT GGCCTAGGAA AAGTGAGCTT GGAAAATGGA 
GAAGCTCCCC GAGAGAGAGG CTCGCCAGTC TCTGGGAGAG CCGAGCCTTC GAGGGAAGCA 
GCAGAAAAGC ATGGTCTGCC GGAGCCCGAC CGAGCCCTGG GTGCCCTAGA GTGCCAGGAA 
TGCGCTGTTC CTCCTGGAGT TGATGAAAAC TCACAGACCA ACGGCATAGC AGACGACAGG 
CAGTCGCTAT CCTCCGCAGA TAATCTGGAA CCAGATGTTC AAGGACACCA AGTGGCTGCT 
AGGTTCTCTC ACATTCTACA GAAGGATGCC TTCCTGGTGT TCCGCTCCCT GTGCAAGCTG 
TCCATGAAGC CCCTTGGGGA AGGGCCACCA GATCCAAAGT CCCACGAGCT TCGTTCCAAG 
GTGGTTTCCC TGCAGCTGCT CCTCTCTGTG CTGCAGAATG CTGGCCCAGT CTTCAGGAGC 
CACGAGATGT TCGTCACTGC CATTAAGCAG TACCTGTGTG TGGCCCTTTC CAAGAATGGC 
GTGTCCTCAG TGCCTGACGT CTTCGAACTC TCGCTTGCTA TTTTTCTTAC TCTTCTTTCA 
AACTTCAAGA TGCACTTGAA AATGCAGATA GAGGTCTTTT TCAAAGAGAT TTTCCTGAAC 
ATTTTAGAAA CATCCACAAG TTCTTTTGAG CACCGCTGGA TGGTCATTCA GACCCTGACG 
AGGATTTGTG CAGATGCCCA GTGCGTTGTG GATATTTATG TCAACTACGA CTGTGACTTA 
AATGCTGCTA ACATTTTCGA GCGACTAGTA AATGACTTAT CCAAAATTGC TCAGGGAAGA 
AGTGGACATG AGCTGGGGAT GACACCTCTA CAGGAGCTCA GTCTGAGGAA GAAAGGCCTG 
GAGTGTCTGG TGTCTATTCT CAAGTGCATG GTGGAGTGGA GCAAGGACCT GTATGTGAAT 
CCCAACCACC AGGCTACCCT GGGTCAGGAG AGGCTCCCAG ATCAGGAAAT GGGGGACGGA 
AAAGGCCTTG ACATGGCAAG ACGGTGCAGC GTGACATCCG TGGAGTCCAC AGTGTCCTCG 
GGGACCCAGA CAGCCATTCA GGATGACCCA GAGCAGTTTG AGGTCATCAA ACAACAGAAG 
GAAATCATTG AGCATGGCAT TGAGCTCTTC AATAAGAAGC CCAAGCGAGG TATCCAGTTT 
CTCCAAGAGC AAGGCATGCT GGGAGCAGCT GTAGAAGATA TTGCCCAATT TCTGCACCAG 
GAGGAGCGGC TGGATTCTAC CCAGGTTGGC GAGTTTCTGG GAGACAGCAC GAGGTTTAAC 
AAGGAGGTGA TGTATGCCTA TGTGGACCAA CTGGATTTCT GTGAAAAAGA GTTCGTCTCA 
GCCCTGAGGA CATTCCTGGA GGGCTTCCGC CTGCCTGGGG AAGCCCAGAA GATTGACCGG 
TTAATGGAGA AGTTTGCTGC ACGATACATA GAATGTAACC AAGGGCAAAC CCTGTTTGCT 
AGTGCGGACA CTGCGTATGT CCTGGCGTAC TCCATCATCA TGCTGACCAC AGACCTGCAC 
AGCCCACAGG TAAAGAATAA GATGACAAAA GAGCAGTATA TTAAAATGAA CCGGGGCATC 
AACGACAGCA AAGACCTGCC TGAGGAGTAC CTGTCCAGCA TCTATGACGA GATAGAGGGC 
AAGAAGATAG CGATGAAGGA GACAAAGGAG CACACAATTG CGACCAAGTC CACCAAGCAG 
AGTGTAGCTA GTGAAAAGCA GAGGCGGCTG CTGTACAATG TGGAGATGGA GCAGATGGCT 
AAAACAGCCA AAGCTCTGAT GGAGGCCGTG AGCCATGCCA AAGCCCCGTT TACCAGCGCC 
ACACACTTGG ACCATGTCCG GCCAATGTTC AAACTGGTGT GGACGCCGCT GCTGGCAGCC 
TATAGTATTG GCCTGCAGAA CTGTGATGAC ACGGAGGTGG CCTCCCTGTG TTTGGAAGGC 
ATCAGGTGTG CAGTCCGCAT TGCCTGCATC TTTGGAATGC AGCTGGAACG AGATGCCTAT 
GTTCAGGCCC TTGCTCGCTT TTCCCTGCTG ACAGCCAGCT CCAGCATCAC AGAAATGAAG 
CAGAAAAACA TCGACACCAT AAAGACACTC ATCACTGTGG CTCACACCGA TGGCAACTAC 
CTTGGCAACT CCTGGCATGA GATCTTGAAG TGCATCAGCC AGCTGGAGCT TGCTCAGCTG 
ATAGGAACTG GGGTGAAGAC TCGCTACCTC TCTGGCTCTG GGCGGGAACG AGAAGGGAGC 
CTGAAGGGCC ACAGTCTGGC AGGAGAAGAA TTCATGGGTC TTGGCCTCGG TAACTTGGTG 
AGTGGTGGTG TAGACAAAAG ACAGATGGCC AGCTTCCAGG AGTCGGTTGG TGAAACAAGC 
TCACAGAGTG TGGTTGTCGC AGTTGACAGA ATTTTTACTG GCTCTACCAG ACTGGATGGA 
AATGCAATAG TTGACTTTGT CCGTTGGCTG TGTGCTGTGT CCATGGATGA GCTGGCTTCT 
CCCCACCATC CTCGAATGTT CAGCCTGCAG AAGATTGTGG AAATATCATA CTACAACATG 
AACAGGATCC GGCTGCAGTG GTCTCGGATA TGGCATGTCA TTGGAGATCA CTTCAATAAG 
GTCGGCTGTA ACCCCAATGA AGATGTGGCC ATCTTCGCAG TTGACTCTCT GCGGCAGCTC 
TCCATGAAGT TCCTGGAGAA GGGAGAATTA GCCAACTTCC GATTTCAGAA GGATTTTCTG 
AGGCCATTTG AACATATTAT GAAGAAAAAC AGGTCCCCGA CCATCCGGGA CATGGTGATC 
CGATGCATCG CTCAGATGGT GAGCTCCCAG GCAGCCAACA TCCGCTCAGG CTGGAAGAAC 
ATCTTTGCTG TGTTCCACCA GGCTGCCTCT GACCACGATG GGAACATTGT GGAGCTGGCC 
TTCCAAACCA CAGGCCACAT CGTCTCAACC ATCTTCCAGC ACCACTTCCC TGCAGCCATC 
GACTCCTTCC AGGATGCTGT GAAGTGCTTG TCTGAGTTCG CCTGCAATGC AGCTTTCCCT 
GACACCAGCA TGGAGGCCAT CCGGCTCATC CGCTTCTGTG GGAAATACGT CTCAGAGAGG 
CCTCGGGTGC TGCAAGAGTA CACAAGTGAT GATATGAATG TGGCTCCTGG TGACAGGGTC 
TGGGTCAGAG GCTGGTTTCC CATCCTGTTT GAACTCTCCT GCATCATTAA CAGATGCAAG 
TTAGATGTAC GCACAAGGGG ACTCACGGTC ATGTTTGAGA TTATGAAGAG CTATGGCCAC 
ACCTTTGCAA AGCACTGGTG GCAGGACCTG TTCAGAATCG TGTTTCGAAT TTTTGACAAC 
ATGAAACTCC CTGAGCAACA GTCAGAGAAA TCAGAGTGGA TGACGACCAC GTGCAATCAT 
GCACTGTACG CTATTTGTGA CGTATTCACC CAGTTCTACG AAGCTTTGCA TGAAGTGCTT 
CTCTCTGATG TGTTTGCGCA GCTGCAGTGG TGCGTGAAAC AAGATAATGA GCAGTTGGCC 
CGATCAGGTA CGAACTGCTT AGAGAACCTA GTGATATCCA ACGGCGAGAA GTTCAGCCCT 
GCTGTCTGGG ATGAAACCTG CAATTGCATG TTGGATATTT TCAAAACCAC CATCCCACAT 
GTTTTGCTGA CGTGGAGACC TGCGGGGATG GAGGAGGAGG TGTCAGATAG ACATCTGGAT 
GTGGACCTAG ACCGCCAGTC TTTAAGCAGC ATAGACAGAA ACGCCTCCGA GAGAGGACAG 
AGCCAGCTGT CCAACCCCAC TGACGACAGC TGGAAGGGGG CACCGTATGC ACATCAGAAA 
CTGCTGGCCA GCCTCCTCAT CAAGTGTGTG GTTCAGCTGG AGCTGATCCA GACCATTGAC 
AACATCGTGT TCTACCCTGC CACCAGCAAG AAGGAGGATG CGGAGCACAT GGTTGCTGCC 
CAGCAAGACA CCCTCGATGC AGAGATCCAC ATCGAGACGG AGAATCAGGG CATGTACAAG 
TTCATGTCCT CCCAGCACCT CTTCAAGCTG CTGGACTGCC TGCAGGAGTC CCACTCCTTC 
TCCAAAGCCT TCAACTCCAA CTATGAGCAG CGGACTGTCC TCTGGCGAGC TGGCTTCAAG 
GGCAAGTCCA AACCCAATCT TCTCAAACAA GAAACCAGCA GCCTGGCCTG TTGTCTAAGG 
ATCCTGTTTA GAATGTACGT TGATGAGAAC CGCAGGGATT CCTGGGACGA AATACAGCAG 
CGACTTCTAA GAGTGTGCAG TGAAGCACTT GCCTATTTCA TTACTGTGAA CTCCGAGAGC 
CATCGGGAAG CATGGACGAG CCTCCTGCTG CTACTTCTAA CCAAAACCCT CAAGATAAGC 
GATGAAAAGT TCAAAGCACA CGCGTCAATG TACTACCCCT ACCTGTGTGA AATTATGCAG 
TTTGACCTGA TCCCTGAGCT CCGAGCAGTT CTGCGAAAGT TCTTCCTGCG GATAGGCCTG 
GTGTATAAGA TATGGATTCC AGAAGAGCCG TCGCAAGTGC CAGCAGCACT GTCATCGACC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Filamin A regulates neuronal migration through brefeldin A-inhibited guanine exchange factor 2-dependent Arf1 activation.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(40)15735-46(2013 Oct)
Zhang J,Neal J,Lian G,Hu J,Lu J,Sheen V

The Sec7 guanine nucleotide exchange factor GBF1 regulates membrane recruitment of BIG1 and BIG2 guanine nucleotide exchange factors to the trans-Golgi network (TGN).
The Journal of biological chemistry288(16)11532-45(2013 Apr)
Lowery J,Szul T,Styers M,Holloway Z,Oorschot V,Klumperman J,Sztul E

Brefeldin A-inhibited guanine exchange factor 2 regulates filamin A phosphorylation and neuronal migration.
The Journal of neuroscience : the official journal of the Society for Neuroscience32(36)12619-29(2012 Sep)
Zhang J,Neal J,Lian G,Shi B,Ferland RJ,Sheen V

Early embryonic lethality in gene trap mice with disruption of the Arfgef2 gene.
The International journal of developmental biology54(8-9)1259-66(2010)
Grzmil P,Enkhbaatar Z,Gundsambuu B,Oidovsambuu O,Yalcin S,Wolf S,Engel W,Neesen J

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Arfgef2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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