IFI30 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	IFI30
Entrez Gene ID	10437
Full Name	IFI30, lysosomal thiol reductase
Synonyms	GILT,IFI-30,IP-30,IP30
General protein information	Preferred Names IFI30, lysosomal thiol reductase Names gamma-interferon-inducible lysosomal thiol reductase gamma-interferon-inducible protein IP-30 interferon gamma-inducible protein 30 preproprotein interferon, gamma-inducible protein 30 legumaturain
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.11
Summary	The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. [provided by RefSeq, Jul 2008].
Disorder MIM:	604664

mRNA and Protein(s)

mRNA	Protein	Name
NM_006332.5	NP_006323.2	gamma-interferon-inducible lysosomal thiol reductase preproprotein
NM_006332.4	NP_006323.2	gamma-interferon-inducible lysosomal thiol reductase preproprotein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ifi30	NP_075552.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009747	XP_318831.4
Canis lupus familiaris (dog)	LOC476669	XP_533874.3
Pan troglodytes (chimpanzee)	IFI30	XP_001162304.2
Bos taurus (cattle)	IFI30	NP_001094721.1
Oryza sativa (rice)	Os03g0295800	NP_001049827.1
Arabidopsis thaliana (thale cress)	AT1G07080	NP_563779.1
Homo sapiens (human)	IFI30	NP_006323.2
	LOC719379	XP_001114116.2
Rattus norvegicus (Norway rat)	Ifi30	NP_001025197.1
Gallus gallus (chicken)	IFI30	XP_004948968.1
Drosophila melanogaster (fruit fly)	CG41378	NP_001163836.1
Xenopus tropicalis (tropical clawed frog)	ifi30	NP_001017196.1

Gene Ontology
Bibliography

Related articles in PubMed

Autophagy-dependent crosstalk between GILT and PAX-3 influences radiation sensitivity of human melanoma cells.
Hathaway-Schrader JD, Doonan BP, Hossain A, Radwan FFY, Zhang L, Haque A
Journal of cellular biochemistry119(2)2212-2221(2018 Feb)

Gamma-interferon-inducible, lysosome/endosome-localized thiolreductase, GILT, has anti-retroviral activity and its expression is counteracted by HIV-1.
Kubo Y, Izumida M, Yashima Y, Yoshii-Kamiyama H, Tanaka Y, Yasui K, Hayashi H, Matsuyama T
Oncotarget7(44)71255-71273(2016 Nov)

Gamma-interferon-inducible lysosomal thiol reductase is upregulated in human melanoma.
Nguyen J, Bernert R, In K, Kang P, Sebastiao N, Hu C, Hastings KT
Melanoma research26(2)125-37(2016 Apr)

Diverse cellular and organismal functions of the lysosomal thiol reductase GILT.
Rausch MP, Hastings KT
Molecular immunology68(2 Pt A)124-8(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GILT expression is anticipated to result in improved presentation of melanoma antigens and more effective antimelanoma T-cell responses.
Title: Gamma-interferon-inducible lysosomal thiol reductase is upregulated in human melanoma.

The lysosomal thiol reductase GILT expressed by antigen-presenting cells has diverse cellular and organismal functions. (Review)
Title: Diverse cellular and organismal functions of the lysosomal thiol reductase GILT.

this review discusses recent studies that have advanced our understanding of the role of GILT in antigen processing and revealed surprising new functions for the enzyme.[review]
Title: Expanding roles for GILT in immunity.

Single nucleotide polymorphism of the interferon-gamma-inducible protein 30 gene is associated with hyperglycemia in severely obese individuals.
Title: A polymorphism of the interferon-gamma-inducible protein 30 gene is associated with hyperglycemia in severely obese individuals.

GILT is required for efficient histocompatiblity class II-restricted processing of melanoma antigen tyrosinase-related protein 1 epitope in vitro and accelerates the onset of vitiligo in TRP1-specific T-cell receptor transgenic mice.
Title: GILT accelerates autoimmunity to the melanoma antigen tyrosinase-related protein 1.

The following IFI30 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IFI30 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00697	NM_006332.5 Latest version!	Homo sapiens IFI30 lysosomal thiol reductase (IFI30), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu00697	NM_006332.4	Homo sapiens IFI30, lysosomal thiol reductase (IFI30), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IFI30 ( NM_006332.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_006323.2 Homo sapiens IFI30 lysosomal thiol reductase (IFI30), mRNA.

Price & Availability↑

CloneID	OHu00697
Clone ID Related Accession (Same CDS sequence)	NM_006332.5 , NM_006332.4
Accession Version	NM_006332.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	gamma-interferon-inducible lysosomal thiol reductase preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC021136.1. On Nov 23, 2018 this sequence version replaced NM_006332.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031020.1, SRR5189658.213581.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000407280.4/ ENSP00000384886.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACCCTGT CGCCACTTCT GCTGTTCCTG CCACCGCTGC TGCTGCTGCT GGACGTCCCC 
ACGGCGGCGG TGCAGGCGTC CCCTCTGCAA GCGTTAGACT TCTTTGGGAA TGGGCCACCA 
GTTAACTACA AGACAGGCAA TCTATACCTG CGGGGGCCCC TGAAGAAGTC CAATGCACCG 
CTTGTCAATG TGACCCTCTA CTATGAAGCA CTGTGCGGTG GCTGCCGAGC CTTCCTGATC 
CGGGAGCTCT TCCCAACATG GCTGTTGGTC ATGGAGATCC TCAATGTCAC GCTGGTGCCC 
TACGGAAACG CACAGGAACA AAATGTCAGT GGCAGGTGGG AGTTCAAGTG CCAGCATGGA 
GAAGAGGAGT GCAAATTCAA CAAGGTGGAG GCCTGCGTGT TGGATGAACT TGACATGGAG 
CTAGCCTTCC TGACCATTGT CTGCATGGAA GAGTTTGAGG ACATGGAGAG AAGTCTGCCA 
CTATGCCTGC AGCTCTACGC CCCAGGGCTG TCGCCAGACA CTATCATGGA GTGTGCAATG 
GGGGACCGCG GCATGCAGCT CATGCACGCC AACGCCCAGC GGACAGATGC TCTCCAGCCA 
CCACACGAGT ATGTGCCCTG GGTCACCGTC AATGGGAAAC CCTTGGAAGA TCAGACCCAG 
CTCCTTACCC TTGTCTGCCA GTTGTACCAG GGCAAGAAGC CGGATGTCTG CCCTTCCTCA 
ACCAGCTCCC TCAGGAGTGT TTGCTTCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006323.2
CDS	30..782
Translation	MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK

Target ORF information:

RefSeq Version	NM_006332.5
Organism	Homo sapiens(human)
Definition	Homo sapiens IFI30 lysosomal thiol reductase (IFI30), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006332.5

ORF Insert Sequence:

ATGACCCTGT CGCCACTTCT GCTGTTCCTG CCACCGCTGC TGCTGCTGCT GGACGTCCCC 
ACGGCGGCGG TGCAGGCGTC CCCTCTGCAA GCGTTAGACT TCTTTGGGAA TGGGCCACCA 
GTTAACTACA AGACAGGCAA TCTATACCTG CGGGGGCCCC TGAAGAAGTC CAATGCACCG 
CTTGTCAATG TGACCCTCTA CTATGAAGCA CTGTGCGGTG GCTGCCGAGC CTTCCTGATC 
CGGGAGCTCT TCCCAACATG GCTGTTGGTC ATGGAGATCC TCAATGTCAC GCTGGTGCCC 
TACGGAAACG CACAGGAACA AAATGTCAGT GGCAGGTGGG AGTTCAAGTG CCAGCATGGA 
GAAGAGGAGT GCAAATTCAA CAAGGTGGAG GCCTGCGTGT TGGATGAACT TGACATGGAG 
CTAGCCTTCC TGACCATTGT CTGCATGGAA GAGTTTGAGG ACATGGAGAG AAGTCTGCCA 
CTATGCCTGC AGCTCTACGC CCCAGGGCTG TCGCCAGACA CTATCATGGA GTGTGCAATG 
GGGGACCGCG GCATGCAGCT CATGCACGCC AACGCCCAGC GGACAGATGC TCTCCAGCCA 
CCACACGAGT ATGTGCCCTG GGTCACCGTC AATGGGAAAC CCTTGGAAGA TCAGACCCAG 
CTCCTTACCC TTGTCTGCCA GTTGTACCAG GGCAAGAAGC CGGATGTCTG CCCTTCCTCA 
ACCAGCTCCC TCAGGAGTGT TTGCTTCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFI30 ( NM_006332.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006323.2 Homo sapiens IFI30, lysosomal thiol reductase (IFI30), mRNA.

Price & Availability↑

CloneID	OHu00697
Clone ID Related Accession (Same CDS sequence)	NM_006332.5 , NM_006332.4
Accession Version	NM_006332.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	gamma-interferon-inducible lysosomal thiol reductase preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL558891.3, BC021136.1 and AI022513.1. On Jul 12, 2013 this sequence version replaced NM_006332.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL558891.3, SRR1163655.251366.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCTGT CGCCACTTCT GCTGTTCCTG CCACCGCTGC TGCTGCTGCT GGACGTCCCC 
ACGGCGGCGG TGCAGGCGTC CCCTCTGCAA GCGTTAGACT TCTTTGGGAA TGGGCCACCA 
GTTAACTACA AGACAGGCAA TCTATACCTG CGGGGGCCCC TGAAGAAGTC CAATGCACCG 
CTTGTCAATG TGACCCTCTA CTATGAAGCA CTGTGCGGTG GCTGCCGAGC CTTCCTGATC 
CGGGAGCTCT TCCCAACATG GCTGTTGGTC ATGGAGATCC TCAATGTCAC GCTGGTGCCC 
TACGGAAACG CACAGGAACA AAATGTCAGT GGCAGGTGGG AGTTCAAGTG CCAGCATGGA 
GAAGAGGAGT GCAAATTCAA CAAGGTGGAG GCCTGCGTGT TGGATGAACT TGACATGGAG 
CTAGCCTTCC TGACCATTGT CTGCATGGAA GAGTTTGAGG ACATGGAGAG AAGTCTGCCA 
CTATGCCTGC AGCTCTACGC CCCAGGGCTG TCGCCAGACA CTATCATGGA GTGTGCAATG 
GGGGACCGCG GCATGCAGCT CATGCACGCC AACGCCCAGC GGACAGATGC TCTCCAGCCA 
CCACACGAGT ATGTGCCCTG GGTCACCGTC AATGGGAAAC CCTTGGAAGA TCAGACCCAG 
CTCCTTACCC TTGTCTGCCA GTTGTACCAG GGCAAGAAGC CGGATGTCTG CCCTTCCTCA 
ACCAGCTCCC TCAGGAGTGT TTGCTTCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006323.2
CDS	63..815
Translation	MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK

Target ORF information:

RefSeq Version	NM_006332.4
Organism	Homo sapiens(human)
Definition	Homo sapiens IFI30, lysosomal thiol reductase (IFI30), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006332.4

ORF Insert Sequence:

ATGACCCTGT CGCCACTTCT GCTGTTCCTG CCACCGCTGC TGCTGCTGCT GGACGTCCCC 
ACGGCGGCGG TGCAGGCGTC CCCTCTGCAA GCGTTAGACT TCTTTGGGAA TGGGCCACCA 
GTTAACTACA AGACAGGCAA TCTATACCTG CGGGGGCCCC TGAAGAAGTC CAATGCACCG 
CTTGTCAATG TGACCCTCTA CTATGAAGCA CTGTGCGGTG GCTGCCGAGC CTTCCTGATC 
CGGGAGCTCT TCCCAACATG GCTGTTGGTC ATGGAGATCC TCAATGTCAC GCTGGTGCCC 
TACGGAAACG CACAGGAACA AAATGTCAGT GGCAGGTGGG AGTTCAAGTG CCAGCATGGA 
GAAGAGGAGT GCAAATTCAA CAAGGTGGAG GCCTGCGTGT TGGATGAACT TGACATGGAG 
CTAGCCTTCC TGACCATTGT CTGCATGGAA GAGTTTGAGG ACATGGAGAG AAGTCTGCCA 
CTATGCCTGC AGCTCTACGC CCCAGGGCTG TCGCCAGACA CTATCATGGA GTGTGCAATG 
GGGGACCGCG GCATGCAGCT CATGCACGCC AACGCCCAGC GGACAGATGC TCTCCAGCCA 
CCACACGAGT ATGTGCCCTG GGTCACCGTC AATGGGAAAC CCTTGGAAGA TCAGACCCAG 
CTCCTTACCC TTGTCTGCCA GTTGTACCAG GGCAAGAAGC CGGATGTCTG CCCTTCCTCA 
ACCAGCTCCC TCAGGAGTGT TTGCTTCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Autophagy-dependent crosstalk between GILT and PAX-3 influences radiation sensitivity of human melanoma cells.
Journal of cellular biochemistry119(2)2212-2221(2018 Feb)
Hathaway-Schrader JD,Doonan BP,Hossain A,Radwan FFY,Zhang L,Haque A

Gamma-interferon-inducible, lysosome/endosome-localized thiolreductase, GILT, has anti-retroviral activity and its expression is counteracted by HIV-1.
Oncotarget7(44)71255-71273(2016 Nov)
Kubo Y,Izumida M,Yashima Y,Yoshii-Kamiyama H,Tanaka Y,Yasui K,Hayashi H,Matsuyama T

Gamma-interferon-inducible lysosomal thiol reductase is upregulated in human melanoma.
Melanoma research26(2)125-37(2016 Apr)
Nguyen J,Bernert R,In K,Kang P,Sebastiao N,Hu C,Hastings KT

Diverse cellular and organismal functions of the lysosomal thiol reductase GILT.
Molecular immunology68(2 Pt A)124-8(2015 Dec)
Rausch MP,Hastings KT

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IFI30 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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