AFDN cDNA ORF clone, Gallus gallus(chicken)

The following AFDN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFDN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa33794 XM_015284344.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33795 XM_015284345.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33796 XM_015284346.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33797 XM_015284347.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33798 XM_015284348.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33799 XM_015284349.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33800 XM_015284350.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33801 XM_015284351.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33802 XM_015284352.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33803 XM_015284353.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33804 XM_015284354.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33805 XM_015284355.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33806 XM_015284356.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33807 XM_015284357.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33808 XM_015284358.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33809 XM_015284359.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33810 XM_015284360.2
Latest version!
Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
View Old Accession Versions >>
OGa33799 XM_015284349.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33801 XM_015284351.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33803 XM_015284353.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33796 XM_015284346.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33797 XM_015284347.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33808 XM_015284358.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33810 XM_015284360.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33794 XM_015284344.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33804 XM_015284354.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33795 XM_015284345.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33805 XM_015284355.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33798 XM_015284348.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33806 XM_015284356.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33800 XM_015284350.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33802 XM_015284352.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33807 XM_015284357.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa33809 XM_015284359.1 Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa33794
    Clone ID Related Accession (Same CDS sequence) XM_015284344.2 , XM_015284344.1
    Accession Version XM_015284344.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5778bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284344.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGTCTGTACA GACCTCCACT TCCACGGGAA
    TATGAACTCC CATCCCCAAC CCCTTCATCT AACGCTCCTC CTCCCCCACC TCAGCGAAAC
    ACCTCTTACC TCAAAACACA GGTCCTCTCC CCTGACACGA TTTATACTGC CAAGTTTGTT
    GCATACAATG ATGATGAGGA GGAGGAGGAT TCCAATCTAG CAGGTCAGGA TAAGTACTCC
    AGCACAAGAA AATCTTATGG TGACTTTCCT CCTGCCACCC TTAAACCACA GCAGCCCTCC
    CGCCAGCCTC GCCCAGTTTC AGATGGCCTC TTTCTTTCCA ACTCATTCCA GTCATCTCCA
    GTCAATGCCA ACAGTACTGC TGCCAAAAAA AGCCAGCCCC CGCCCCCACC AAACAAACCG
    AGTTTCATCC ATGCCAGCAG CTCTAAAGGA ATAAATTCAT ACACTGGATC TTCGGGAGCA
    ACGGTTGGGG CCCATGAAGT CTATCGGGAT CCAAGAGACA AACGATCTAA AAGTCAAGAT
    ACAGATTTAC CTGGAGCACC AGAAAATCTG ACGTTCAGGG AACGCCAGCG ACTTTTTTCA
    CAGGGTCAAG ATGTATCCAA TAAAGTAAAA GCCTCTAGGA AACTAATGGA ACTGGAAAAT
    GAGTTGAACA CGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139830.1
    CDS2885..8662
    Translation

    Target ORF information:

    RefSeq Version XM_015284344.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284344.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGTCTGTACA GACCTCCACT TCCACGGGAA
    TATGAACTCC CATCCCCAAC CCCTTCATCT AACGCTCCTC CTCCCCCACC TCAGCGAAAC
    ACCTCTTACC TCAAAACACA GGTCCTCTCC CCTGACACGA TTTATACTGC CAAGTTTGTT
    GCATACAATG ATGATGAGGA GGAGGAGGAT TCCAATCTAG CAGGTCAGGA TAAGTACTCC
    AGCACAAGAA AATCTTATGG TGACTTTCCT CCTGCCACCC TTAAACCACA GCAGCCCTCC
    CGCCAGCCTC GCCCAGTTTC AGATGGCCTC TTTCTTTCCA ACTCATTCCA GTCATCTCCA
    GTCAATGCCA ACAGTACTGC TGCCAAAAAA AGCCAGCCCC CGCCCCCACC AAACAAACCG
    AGTTTCATCC ATGCCAGCAG CTCTAAAGGA ATAAATTCAT ACACTGGATC TTCGGGAGCA
    ACGGTTGGGG CCCATGAAGT CTATCGGGAT CCAAGAGACA AACGATCTAA AAGTCAAGAT
    ACAGATTTAC CTGGAGCACC AGAAAATCTG ACGTTCAGGG AACGCCAGCG ACTTTTTTCA
    CAGGGTCAAG ATGTATCCAA TAAAGTAAAA GCCTCTAGGA AACTAATGGA ACTGGAAAAT
    GAGTTGAACA CGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33794
    Clone ID Related Accession (Same CDS sequence) XM_015284344.2 , XM_015284344.1
    Accession Version XM_015284344.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5778bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGTCTGTACA GACCTCCACT TCCACGGGAA
    TATGAACTCC CATCCCCAAC CCCTTCATCT AACGCTCCTC CTCCCCCACC TCAGCGAAAC
    ACCTCTTACC TCAAAACACA GGTCCTCTCC CCTGACACGA TTTATACTGC CAAGTTTGTT
    GCATACAATG ATGATGAGGA GGAGGAGGAT TCCAATCTAG CAGGTCAGGA TAAGTACTCC
    AGCACAAGAA AATCTTATGG TGACTTTCCT CCTGCCACCC TTAAACCACA GCAGCCCTCC
    CGCCAGCCTC GCCCAGTTTC AGATGGCCTC TTTCTTTCCA ACTCATTCCA GTCATCTCCA
    GTCAATGCCA ACAGTACTGC TGCCAAAAAA AGCCAGCCCC CGCCCCCACC AAACAAACCG
    AGTTTCATCC ATGCCAGCAG CTCTAAAGGA ATAAATTCAT ACACTGGATC TTCGGGAGCA
    ACGGTTGGGG CCCATGAAGT CTATCGGGAT CCAAGAGACA AACGATCTAA AAGTCAAGAT
    ACAGATTTAC CTGGAGCACC AGAAAATCTG ACGTTCAGGG AACGCCAGCG ACTTTTTTCA
    CAGGGTCAAG ATGTATCCAA TAAAGTAAAA GCCTCTAGGA AACTAATGGA ACTGGAAAAT
    GAGTTGAACA CGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139830.1
    CDS33..5810
    Translation

    Target ORF information:

    RefSeq Version XM_015284344.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284344.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGTCTGTACA GACCTCCACT TCCACGGGAA
    TATGAACTCC CATCCCCAAC CCCTTCATCT AACGCTCCTC CTCCCCCACC TCAGCGAAAC
    ACCTCTTACC TCAAAACACA GGTCCTCTCC CCTGACACGA TTTATACTGC CAAGTTTGTT
    GCATACAATG ATGATGAGGA GGAGGAGGAT TCCAATCTAG CAGGTCAGGA TAAGTACTCC
    AGCACAAGAA AATCTTATGG TGACTTTCCT CCTGCCACCC TTAAACCACA GCAGCCCTCC
    CGCCAGCCTC GCCCAGTTTC AGATGGCCTC TTTCTTTCCA ACTCATTCCA GTCATCTCCA
    GTCAATGCCA ACAGTACTGC TGCCAAAAAA AGCCAGCCCC CGCCCCCACC AAACAAACCG
    AGTTTCATCC ATGCCAGCAG CTCTAAAGGA ATAAATTCAT ACACTGGATC TTCGGGAGCA
    ACGGTTGGGG CCCATGAAGT CTATCGGGAT CCAAGAGACA AACGATCTAA AAGTCAAGAT
    ACAGATTTAC CTGGAGCACC AGAAAATCTG ACGTTCAGGG AACGCCAGCG ACTTTTTTCA
    CAGGGTCAAG ATGTATCCAA TAAAGTAAAA GCCTCTAGGA AACTAATGGA ACTGGAAAAT
    GAGTTGAACA CGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33795
    Clone ID Related Accession (Same CDS sequence) XM_015284345.1 , XM_015284345.2
    Accession Version XM_015284345.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAAGAA GATTAGATGT GGATGAGAAG CCTCTTGTTG TACAATTAAA CTGGAACAAA
    GATGATCGAG AAGGAAGGTT TGTTCTCAAG AATGAAAATG ATACGCTTCC TCCAAAGAAG
    GCTCAGAGTA ATGGCCCTGA AAAACAAGAA AAAGAAGGAG TAATACAGAA TTTCAAACGA
    ACTCTCTCAA AGAAAGAAAA GAAAGAGAAG AAGAGGCGTG AAAAAGAGGC ATTGCGACAA
    GCATCAGATA AAGATGATCG ACTTCTTCAT GGGGATGATA TTGAGAATTC TCGCCTTGCA
    GCTGAGGTTT ACAAAGATAT GCCTGAGACC AGCTTCACTC GCACAATATC TAATCCTGAG
    GTGGTTATGA AACGACGGCG GCAGCAAAAA CTAGAGAAGA GAATGCAGGA ATTTCGCAGT
    TCTGATGGCA GACCAGACTC AGGTGGGACG CTGAGAATTT ATGCGGACAG TTTAAAACCA
    AATATACCAT ACAAGACAAT CCTGTTGTCA ACTACAGATA CTGCAGACTT TGCAGTTATT
    GAAGCACTGG AGAAATATGG CTTGGAGAAG GAAAACCCCA AGGATTATTG TATTGCTCGT
    GTCATTCTGC CTCCTGGTGC TCAGCACTCT GATGATAAAG GTGCTAAAGA AACTATTCTA
    GATGATGATG AGTGTCCACT TCAGATATTC AGGGAATGGC CTAGTGATAA AGGAATACTA
    GTCTTTCAGT TGAAAAGGCG GCCTCCTGAT TATGTCCCAA AGAAATCCAA GAAAATAGCT
    GATGGAAAGC CATTAAAGGG GAAGGAAAGA GTTGACGGGT CTGGCTACGG CTCTTGCCTT
    CCTCCTGAGA AACTACCATA CCTGGTAGAA TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACGCTTA TGAAGATGGT TCTGACTCCA GAGATAAACC AAAGCTTTAT
    CGTCTACAAT TAAGTGTCAC TGAAGTTGGA ACTGAAAAAT TTGATGACAA TTCCATTCAG
    TTATTTGGTC CAGGAATTCA GCCTCATCAT TGTGATCTTA CTAATATGGA TGGAGTTGTT
    ACTGTAACCC CAAGAAACAT TGATGCGGAA ACGTATGTAG AAGGTCAGCG TATTTCAGAA
    ACCACTATGC TGCAAAGTGG AATGAAGGTT CAGTTTGGGT CATCTCATGT GTTTAAGTTT
    GTGGATCCCA CTCAGGACCA TATTATTACT AAAAGACCTA TTGATGCAAG CCTTATGGTC
    AAAGGTCCAA GGCATAAAGC TGGAGCTGTT CAGGAAACCA CGTTTGATCT GGAGGGAGAT
    GTTCACAGTG GAACAGCGTT ACCAGCGAGC AAGAGCACCA GCAGGCTTGA TGGTGACAGA
    ACATCATCAG CATCCAGCAC AGCTGAACGA GGAATGGTGA AGCCCATGAT TAGAATAGAA
    CAGCAGCAAG ATTATCGCAG ACAGGAAAGC AGACCTCAGG ATACCCATGG ACCAGAACTG
    ATCCTACCTG CCAGTATTGA ATTCAGGGAA AGCTCTGAAG ATGCCTTCTT ATCTGCCATT
    ATAAATTATA CAAATAGCTC GACAGTTCAT TTTAAACTGT CACCCACGTA TGTTTTGTAT
    ATGACATGTC GGTATGTATT GTCAAGCCAG TACAGACCTG ACATCACTCC TACTGAGCGT
    ACTCACAAAG TTATTGCAAT AGTCAACAAG ATGGTGAATA TGATGGAAGG AGTCATACAG
    GAGGTAGACC AGGTTGATCA GAAACAGAAG AATATTGCTG GGGCACTTGC CTTTTGGATG
    GCAAATGCGT CTGAATTGCT GAATTTTATA AAGCAAGACC GTGATCTGAG CCGAATCACT
    GTGGATGCTC AAGATGTTTT GGCGCACTTG GTTCAAATGG CATTCAAGTA CCTAGTTCAT
    TGTCTGCAGT CAGAGCTTAA TAACTACATG CCAGCATTTC TTGATGATCC AGAGGAAAAT
    AATCCACAGA GGCCTAAAAT AGATGATGTG CTGCACACAT TGACTGGAGC TATGTCCTTG
    TTGCGACGAT GTAGAGTGAA TGCTGCACTG ACTATACAGC TCTTCTCCCA GTTGTTTCAT
    TTTATCAACA TGTGGCTTTT TAACAGACTA GTTACGGCTC CAGATTCAGG GTTATGTTCC
    CACTACTGGG GAGCTATTGT TCGTCAGCAG TTGGGTCATA TTGAGGCCTG GGCAGAAAAA
    CAGGGTTTAG AATTGGCTGC TGATTGCCAC TTAAGCAGAA TAGTGCAGGC AACCACATTG
    CTCACCATGG ACAAGTACTC ACATGCTGAT GTTCCAAATA TTAACAGTAC TTGCTTTAAG
    TTGAATTCTC TACAGCTACA AGCTTTGTTG CAGAATTATC ACTGTGCTCC AGATGAACCG
    CTTATACCAA CAGAACTGAT AGAGAATGTA GTAACTGTTG CAGAAAATAC GGCTGATGAA
    CTTGCTCGCA GTGATGGCAG AGAAGTCCAA TTGGAAGAGG ATCCTGACTT ACAACTCCCT
    TTTCTTTTAC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAATGTTCC AAGTGGTTTA
    CAAGAATTTT TGGATCCCCT CTGTCAGAGA GGTTTTTGTA GGTTAATACC TCATACACGG
    TCACCAGGCA CATGGACAAT ATACTTTGAA GGCGCAGACT ATGAAAGCCA CCTGTCACAT
    GAGAATGCTG AGCTAGCTCA GCCTTTGAGG AAAGAACCTG AGATTATAAC TGTAACACTA
    AAAAAACAAA ATGGAATGGG CCTCAGCATT GTTGCTGCCA AGGGTGCTGG TCAAGACAAA
    CTTGGAATAT ATGTCAAGTC TGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGTCGTCTG
    GCAGCAGGGG ATCAGCTGCT CAGTGTGGAT GGTCGAAGCT TGGTGGGGCT GTCCCAGGAA
    AGGGCAGCAG AACTTATGAC GAGGACAGGT TCTGTTGTAA CACTGGAAGT AGCAAAACAA
    GGAGCAATAT ACCACGGTCT GGCAACCCTG CTAAATCAGC CATCTCCAAT GATGCAAAGA
    GTTTCTGACC GCCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGTTT TGAGCTGTAT
    AACAACTCCA CGCAAAATGG GTCACCTGAG AGCCCCCAGC TGCCATGGAC GGAGTACAGT
    GAACCAAAGA AGCTGCCAGG AGATGACAGA TTAATGAAGA ACAGAGCCGA TCATCGCTCC
    AGCCCCAACG TAGCAAATCA GCCTCCAAGC CCTGGGGGGA AGAATGCTTA TGCATCTGGA
    ACTACCACCA AAATAACCTC AGTCTCGACT GGAAATCTTT GTACAGAGGA GCAAACTCTG
    CCTCCACGAC CTGAAGCTTA TCCTATCCCA ACACAGACCT ATACCAGAGA ATATTTCACA
    TTCCCAGCTT CAAAATCCCA GGATCGAATG GGTCCAGCTC AGAGTCAGTG GCCAAATTAT
    GAAGAGAAGC CACAGATCCA GGCAGAAAGT AATCACTCAA TCAATACTAC AATGCAGCGC
    GTGGCTCGAT CCCAGGAAGA ACTCCGAGAT AAAGTTTTCC AGCCAGAGAG GAATCGTTTG
    GAAGTTGTTA TCCGGAAGGA CATAGAATAC ATTGATCGGA AGTCAGACAG CGATCAGTGG
    ATGAATTCAT CTGTGGATTC TAGCACATCT AGCCAAGAGC ACCTGAACCA TACATCCAAA
    CCTGTTTCAC CTGGTTCTTG CTTAACTAAA AGTGGACCTG GCCGTTGGAA AACTCCAGCT
    ACGAGCCAGC CAACTCCAGT GGCCGTTTCC CAGCCCATCA GAACAGACCT TCCTCCTCCG
    CCGCCACCTC CTCCGGTGCA TTATGCAGCT GAATTTGATG AACCACAAAT GGATTTGCCT
    CTGCCTCCCC CTCCTCCACC GAACCAAGTA GGAGCACAAG CATCTTCTCA GGTGGCTGCT
    GCTGAGCGTA AAAAACGAGA AGAGCATCAG AGGTGGTATG AAAAGGAAAA GGCACGTTTG
    GAAGAAGAAC GAGAAAGGAA ACGCCGTGAG CAAGAGAGAA AACTGGGCCA AATGCGTTCG
    CAGCCACTAA TTCCTCCCCA GCCTGTGGTT CCTCCTGTTT CCCTTCAGCA AGTGAAATCG
    GAAAAAACTT CAACTTTCCA GAGGTCTCAA GAAACAGTCA TTCGAGAGTT GCAGCCGCAA
    CAGCAGCCTC GGACTATTGA GCGGAGAGAT CTGCAGTACA TTACTATCAG CAAGGAAGAA
    TTGTCCTCTA GTGACAGCCT CTCTCCAGAT CCCTGGAAAC GAGATGCCAA GGAGAAACTG
    GAGAAGCAGC AGCAGATGCA CATTGTGGAC ATGCTGAGCA AAGAGATTCA GGATCTTCAG
    AACAAGCCTG ATCGTACAGC TGAAGAGAAC GACCGCCTGC GCAAACTCAT GCTAGAGTGG
    CAGTTCCAAA AGCGACTTCA GGAATCAAAA CAAAAGGATG AAGATGATGA TGAAGAAGAG
    GACGATGATG TGGATACTAT GCTTATCATG CAGCGGCTGG AGGCAGAAAG AAGAGCAAGG
    CAGACTGCTG TGCCAGCAAT CTCTGTTCTA GACTTGTTAC AGGATGAAGA ACGCAGACGA
    CAGCAACAGT TAGAAGAAAT GCGCAAGCGA GAAGCAGAAG ACAGGGCCAG GCAGGAAGAG
    GAGCGCCGAC AGCAAGAGGA GGAGCGAACC AGGAGAGAGG CAGAAGAAAA GAGGCGACAG
    GAAGAGGAGT ATTACAATCG CTTAGAGGCT GAAAGGCGCA GGCAGCACGA TGAAGCAGAA
    CGAAGATTGC TGGAACCAGA CGAGCCTGGT CTGTACAGAC CTCCACTTCC ACGGGAATAT
    GAACTCCCAT CCCCAACCCC TTCATCTAAC GCTCCTCCTC CCCCACCTCA GCGAAACACC
    TCTTACCTCA AAACACAGGT CCTCTCCCCT GACACGATTT ATACTGCCAA GTTTGTTGCA
    TACAATGATG ATGAGGAGGA GGAGGATTCC AATCTAGCAG GTCAGGATAA GTACTCCAGC
    ACAAGAAAAT CTTATGGTGA CTTTCCTCCT GCCACCCTTA AACCACAGCA GCCCTCCCGC
    CAGCCTCGCC CAGTTTCAGA TGGCCTCTTT CTTTCCAACT CATTCCAGTC ATCTCCAGTC
    AATGCCAACA GTACTGCTGC CAAAAAAAGC CAGCCCCCGC CCCCACCAAA CAAACCGAGT
    TTCATCCATG CCAGCAGCTC TAAAGGAATA AATTCATACA CTGGATCTTC GGGAGCAACG
    GTTGGGGCCC ATGAAGTCTA TCGGGATCCA AGAGACAAAC GATCTAAAAG TCAAGATACA
    GATTTACCTG GAGCACCAGA AAATCTGACG TTCAGGGAAC GCCAGCGACT TTTTTCACAG
    GGTCAAGATG TATCCAATAA AGTAAAAGCC TCTAGGAAAC TAATGGAACT GGAAAATGAG
    TTGAACACGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139831.1
    CDS70..5844
    Translation

    Target ORF information:

    RefSeq Version XM_015284345.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284345.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAAGAA GATTAGATGT GGATGAGAAG CCTCTTGTTG TACAATTAAA CTGGAACAAA
    GATGATCGAG AAGGAAGGTT TGTTCTCAAG AATGAAAATG ATACGCTTCC TCCAAAGAAG
    GCTCAGAGTA ATGGCCCTGA AAAACAAGAA AAAGAAGGAG TAATACAGAA TTTCAAACGA
    ACTCTCTCAA AGAAAGAAAA GAAAGAGAAG AAGAGGCGTG AAAAAGAGGC ATTGCGACAA
    GCATCAGATA AAGATGATCG ACTTCTTCAT GGGGATGATA TTGAGAATTC TCGCCTTGCA
    GCTGAGGTTT ACAAAGATAT GCCTGAGACC AGCTTCACTC GCACAATATC TAATCCTGAG
    GTGGTTATGA AACGACGGCG GCAGCAAAAA CTAGAGAAGA GAATGCAGGA ATTTCGCAGT
    TCTGATGGCA GACCAGACTC AGGTGGGACG CTGAGAATTT ATGCGGACAG TTTAAAACCA
    AATATACCAT ACAAGACAAT CCTGTTGTCA ACTACAGATA CTGCAGACTT TGCAGTTATT
    GAAGCACTGG AGAAATATGG CTTGGAGAAG GAAAACCCCA AGGATTATTG TATTGCTCGT
    GTCATTCTGC CTCCTGGTGC TCAGCACTCT GATGATAAAG GTGCTAAAGA AACTATTCTA
    GATGATGATG AGTGTCCACT TCAGATATTC AGGGAATGGC CTAGTGATAA AGGAATACTA
    GTCTTTCAGT TGAAAAGGCG GCCTCCTGAT TATGTCCCAA AGAAATCCAA GAAAATAGCT
    GATGGAAAGC CATTAAAGGG GAAGGAAAGA GTTGACGGGT CTGGCTACGG CTCTTGCCTT
    CCTCCTGAGA AACTACCATA CCTGGTAGAA TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACGCTTA TGAAGATGGT TCTGACTCCA GAGATAAACC AAAGCTTTAT
    CGTCTACAAT TAAGTGTCAC TGAAGTTGGA ACTGAAAAAT TTGATGACAA TTCCATTCAG
    TTATTTGGTC CAGGAATTCA GCCTCATCAT TGTGATCTTA CTAATATGGA TGGAGTTGTT
    ACTGTAACCC CAAGAAACAT TGATGCGGAA ACGTATGTAG AAGGTCAGCG TATTTCAGAA
    ACCACTATGC TGCAAAGTGG AATGAAGGTT CAGTTTGGGT CATCTCATGT GTTTAAGTTT
    GTGGATCCCA CTCAGGACCA TATTATTACT AAAAGACCTA TTGATGCAAG CCTTATGGTC
    AAAGGTCCAA GGCATAAAGC TGGAGCTGTT CAGGAAACCA CGTTTGATCT GGAGGGAGAT
    GTTCACAGTG GAACAGCGTT ACCAGCGAGC AAGAGCACCA GCAGGCTTGA TGGTGACAGA
    ACATCATCAG CATCCAGCAC AGCTGAACGA GGAATGGTGA AGCCCATGAT TAGAATAGAA
    CAGCAGCAAG ATTATCGCAG ACAGGAAAGC AGACCTCAGG ATACCCATGG ACCAGAACTG
    ATCCTACCTG CCAGTATTGA ATTCAGGGAA AGCTCTGAAG ATGCCTTCTT ATCTGCCATT
    ATAAATTATA CAAATAGCTC GACAGTTCAT TTTAAACTGT CACCCACGTA TGTTTTGTAT
    ATGACATGTC GGTATGTATT GTCAAGCCAG TACAGACCTG ACATCACTCC TACTGAGCGT
    ACTCACAAAG TTATTGCAAT AGTCAACAAG ATGGTGAATA TGATGGAAGG AGTCATACAG
    GAGGTAGACC AGGTTGATCA GAAACAGAAG AATATTGCTG GGGCACTTGC CTTTTGGATG
    GCAAATGCGT CTGAATTGCT GAATTTTATA AAGCAAGACC GTGATCTGAG CCGAATCACT
    GTGGATGCTC AAGATGTTTT GGCGCACTTG GTTCAAATGG CATTCAAGTA CCTAGTTCAT
    TGTCTGCAGT CAGAGCTTAA TAACTACATG CCAGCATTTC TTGATGATCC AGAGGAAAAT
    AATCCACAGA GGCCTAAAAT AGATGATGTG CTGCACACAT TGACTGGAGC TATGTCCTTG
    TTGCGACGAT GTAGAGTGAA TGCTGCACTG ACTATACAGC TCTTCTCCCA GTTGTTTCAT
    TTTATCAACA TGTGGCTTTT TAACAGACTA GTTACGGCTC CAGATTCAGG GTTATGTTCC
    CACTACTGGG GAGCTATTGT TCGTCAGCAG TTGGGTCATA TTGAGGCCTG GGCAGAAAAA
    CAGGGTTTAG AATTGGCTGC TGATTGCCAC TTAAGCAGAA TAGTGCAGGC AACCACATTG
    CTCACCATGG ACAAGTACTC ACATGCTGAT GTTCCAAATA TTAACAGTAC TTGCTTTAAG
    TTGAATTCTC TACAGCTACA AGCTTTGTTG CAGAATTATC ACTGTGCTCC AGATGAACCG
    CTTATACCAA CAGAACTGAT AGAGAATGTA GTAACTGTTG CAGAAAATAC GGCTGATGAA
    CTTGCTCGCA GTGATGGCAG AGAAGTCCAA TTGGAAGAGG ATCCTGACTT ACAACTCCCT
    TTTCTTTTAC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAATGTTCC AAGTGGTTTA
    CAAGAATTTT TGGATCCCCT CTGTCAGAGA GGTTTTTGTA GGTTAATACC TCATACACGG
    TCACCAGGCA CATGGACAAT ATACTTTGAA GGCGCAGACT ATGAAAGCCA CCTGTCACAT
    GAGAATGCTG AGCTAGCTCA GCCTTTGAGG AAAGAACCTG AGATTATAAC TGTAACACTA
    AAAAAACAAA ATGGAATGGG CCTCAGCATT GTTGCTGCCA AGGGTGCTGG TCAAGACAAA
    CTTGGAATAT ATGTCAAGTC TGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGTCGTCTG
    GCAGCAGGGG ATCAGCTGCT CAGTGTGGAT GGTCGAAGCT TGGTGGGGCT GTCCCAGGAA
    AGGGCAGCAG AACTTATGAC GAGGACAGGT TCTGTTGTAA CACTGGAAGT AGCAAAACAA
    GGAGCAATAT ACCACGGTCT GGCAACCCTG CTAAATCAGC CATCTCCAAT GATGCAAAGA
    GTTTCTGACC GCCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGTTT TGAGCTGTAT
    AACAACTCCA CGCAAAATGG GTCACCTGAG AGCCCCCAGC TGCCATGGAC GGAGTACAGT
    GAACCAAAGA AGCTGCCAGG AGATGACAGA TTAATGAAGA ACAGAGCCGA TCATCGCTCC
    AGCCCCAACG TAGCAAATCA GCCTCCAAGC CCTGGGGGGA AGAATGCTTA TGCATCTGGA
    ACTACCACCA AAATAACCTC AGTCTCGACT GGAAATCTTT GTACAGAGGA GCAAACTCTG
    CCTCCACGAC CTGAAGCTTA TCCTATCCCA ACACAGACCT ATACCAGAGA ATATTTCACA
    TTCCCAGCTT CAAAATCCCA GGATCGAATG GGTCCAGCTC AGAGTCAGTG GCCAAATTAT
    GAAGAGAAGC CACAGATCCA GGCAGAAAGT AATCACTCAA TCAATACTAC AATGCAGCGC
    GTGGCTCGAT CCCAGGAAGA ACTCCGAGAT AAAGTTTTCC AGCCAGAGAG GAATCGTTTG
    GAAGTTGTTA TCCGGAAGGA CATAGAATAC ATTGATCGGA AGTCAGACAG CGATCAGTGG
    ATGAATTCAT CTGTGGATTC TAGCACATCT AGCCAAGAGC ACCTGAACCA TACATCCAAA
    CCTGTTTCAC CTGGTTCTTG CTTAACTAAA AGTGGACCTG GCCGTTGGAA AACTCCAGCT
    ACGAGCCAGC CAACTCCAGT GGCCGTTTCC CAGCCCATCA GAACAGACCT TCCTCCTCCG
    CCGCCACCTC CTCCGGTGCA TTATGCAGCT GAATTTGATG AACCACAAAT GGATTTGCCT
    CTGCCTCCCC CTCCTCCACC GAACCAAGTA GGAGCACAAG CATCTTCTCA GGTGGCTGCT
    GCTGAGCGTA AAAAACGAGA AGAGCATCAG AGGTGGTATG AAAAGGAAAA GGCACGTTTG
    GAAGAAGAAC GAGAAAGGAA ACGCCGTGAG CAAGAGAGAA AACTGGGCCA AATGCGTTCG
    CAGCCACTAA TTCCTCCCCA GCCTGTGGTT CCTCCTGTTT CCCTTCAGCA AGTGAAATCG
    GAAAAAACTT CAACTTTCCA GAGGTCTCAA GAAACAGTCA TTCGAGAGTT GCAGCCGCAA
    CAGCAGCCTC GGACTATTGA GCGGAGAGAT CTGCAGTACA TTACTATCAG CAAGGAAGAA
    TTGTCCTCTA GTGACAGCCT CTCTCCAGAT CCCTGGAAAC GAGATGCCAA GGAGAAACTG
    GAGAAGCAGC AGCAGATGCA CATTGTGGAC ATGCTGAGCA AAGAGATTCA GGATCTTCAG
    AACAAGCCTG ATCGTACAGC TGAAGAGAAC GACCGCCTGC GCAAACTCAT GCTAGAGTGG
    CAGTTCCAAA AGCGACTTCA GGAATCAAAA CAAAAGGATG AAGATGATGA TGAAGAAGAG
    GACGATGATG TGGATACTAT GCTTATCATG CAGCGGCTGG AGGCAGAAAG AAGAGCAAGG
    CAGACTGCTG TGCCAGCAAT CTCTGTTCTA GACTTGTTAC AGGATGAAGA ACGCAGACGA
    CAGCAACAGT TAGAAGAAAT GCGCAAGCGA GAAGCAGAAG ACAGGGCCAG GCAGGAAGAG
    GAGCGCCGAC AGCAAGAGGA GGAGCGAACC AGGAGAGAGG CAGAAGAAAA GAGGCGACAG
    GAAGAGGAGT ATTACAATCG CTTAGAGGCT GAAAGGCGCA GGCAGCACGA TGAAGCAGAA
    CGAAGATTGC TGGAACCAGA CGAGCCTGGT CTGTACAGAC CTCCACTTCC ACGGGAATAT
    GAACTCCCAT CCCCAACCCC TTCATCTAAC GCTCCTCCTC CCCCACCTCA GCGAAACACC
    TCTTACCTCA AAACACAGGT CCTCTCCCCT GACACGATTT ATACTGCCAA GTTTGTTGCA
    TACAATGATG ATGAGGAGGA GGAGGATTCC AATCTAGCAG GTCAGGATAA GTACTCCAGC
    ACAAGAAAAT CTTATGGTGA CTTTCCTCCT GCCACCCTTA AACCACAGCA GCCCTCCCGC
    CAGCCTCGCC CAGTTTCAGA TGGCCTCTTT CTTTCCAACT CATTCCAGTC ATCTCCAGTC
    AATGCCAACA GTACTGCTGC CAAAAAAAGC CAGCCCCCGC CCCCACCAAA CAAACCGAGT
    TTCATCCATG CCAGCAGCTC TAAAGGAATA AATTCATACA CTGGATCTTC GGGAGCAACG
    GTTGGGGCCC ATGAAGTCTA TCGGGATCCA AGAGACAAAC GATCTAAAAG TCAAGATACA
    GATTTACCTG GAGCACCAGA AAATCTGACG TTCAGGGAAC GCCAGCGACT TTTTTCACAG
    GGTCAAGATG TATCCAATAA AGTAAAAGCC TCTAGGAAAC TAATGGAACT GGAAAATGAG
    TTGAACACGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33795
    Clone ID Related Accession (Same CDS sequence) XM_015284345.1 , XM_015284345.2
    Accession Version XM_015284345.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAAGAA GATTAGATGT GGATGAGAAG CCTCTTGTTG TACAATTAAA CTGGAACAAA
    GATGATCGAG AAGGAAGGTT TGTTCTCAAG AATGAAAATG ATACGCTTCC TCCAAAGAAG
    GCTCAGAGTA ATGGCCCTGA AAAACAAGAA AAAGAAGGAG TAATACAGAA TTTCAAACGA
    ACTCTCTCAA AGAAAGAAAA GAAAGAGAAG AAGAGGCGTG AAAAAGAGGC ATTGCGACAA
    GCATCAGATA AAGATGATCG ACTTCTTCAT GGGGATGATA TTGAGAATTC TCGCCTTGCA
    GCTGAGGTTT ACAAAGATAT GCCTGAGACC AGCTTCACTC GCACAATATC TAATCCTGAG
    GTGGTTATGA AACGACGGCG GCAGCAAAAA CTAGAGAAGA GAATGCAGGA ATTTCGCAGT
    TCTGATGGCA GACCAGACTC AGGTGGGACG CTGAGAATTT ATGCGGACAG TTTAAAACCA
    AATATACCAT ACAAGACAAT CCTGTTGTCA ACTACAGATA CTGCAGACTT TGCAGTTATT
    GAAGCACTGG AGAAATATGG CTTGGAGAAG GAAAACCCCA AGGATTATTG TATTGCTCGT
    GTCATTCTGC CTCCTGGTGC TCAGCACTCT GATGATAAAG GTGCTAAAGA AACTATTCTA
    GATGATGATG AGTGTCCACT TCAGATATTC AGGGAATGGC CTAGTGATAA AGGAATACTA
    GTCTTTCAGT TGAAAAGGCG GCCTCCTGAT TATGTCCCAA AGAAATCCAA GAAAATAGCT
    GATGGAAAGC CATTAAAGGG GAAGGAAAGA GTTGACGGGT CTGGCTACGG CTCTTGCCTT
    CCTCCTGAGA AACTACCATA CCTGGTAGAA TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACGCTTA TGAAGATGGT TCTGACTCCA GAGATAAACC AAAGCTTTAT
    CGTCTACAAT TAAGTGTCAC TGAAGTTGGA ACTGAAAAAT TTGATGACAA TTCCATTCAG
    TTATTTGGTC CAGGAATTCA GCCTCATCAT TGTGATCTTA CTAATATGGA TGGAGTTGTT
    ACTGTAACCC CAAGAAACAT TGATGCGGAA ACGTATGTAG AAGGTCAGCG TATTTCAGAA
    ACCACTATGC TGCAAAGTGG AATGAAGGTT CAGTTTGGGT CATCTCATGT GTTTAAGTTT
    GTGGATCCCA CTCAGGACCA TATTATTACT AAAAGACCTA TTGATGCAAG CCTTATGGTC
    AAAGGTCCAA GGCATAAAGC TGGAGCTGTT CAGGAAACCA CGTTTGATCT GGAGGGAGAT
    GTTCACAGTG GAACAGCGTT ACCAGCGAGC AAGAGCACCA GCAGGCTTGA TGGTGACAGA
    ACATCATCAG CATCCAGCAC AGCTGAACGA GGAATGGTGA AGCCCATGAT TAGAATAGAA
    CAGCAGCAAG ATTATCGCAG ACAGGAAAGC AGACCTCAGG ATACCCATGG ACCAGAACTG
    ATCCTACCTG CCAGTATTGA ATTCAGGGAA AGCTCTGAAG ATGCCTTCTT ATCTGCCATT
    ATAAATTATA CAAATAGCTC GACAGTTCAT TTTAAACTGT CACCCACGTA TGTTTTGTAT
    ATGACATGTC GGTATGTATT GTCAAGCCAG TACAGACCTG ACATCACTCC TACTGAGCGT
    ACTCACAAAG TTATTGCAAT AGTCAACAAG ATGGTGAATA TGATGGAAGG AGTCATACAG
    GAGGTAGACC AGGTTGATCA GAAACAGAAG AATATTGCTG GGGCACTTGC CTTTTGGATG
    GCAAATGCGT CTGAATTGCT GAATTTTATA AAGCAAGACC GTGATCTGAG CCGAATCACT
    GTGGATGCTC AAGATGTTTT GGCGCACTTG GTTCAAATGG CATTCAAGTA CCTAGTTCAT
    TGTCTGCAGT CAGAGCTTAA TAACTACATG CCAGCATTTC TTGATGATCC AGAGGAAAAT
    AATCCACAGA GGCCTAAAAT AGATGATGTG CTGCACACAT TGACTGGAGC TATGTCCTTG
    TTGCGACGAT GTAGAGTGAA TGCTGCACTG ACTATACAGC TCTTCTCCCA GTTGTTTCAT
    TTTATCAACA TGTGGCTTTT TAACAGACTA GTTACGGCTC CAGATTCAGG GTTATGTTCC
    CACTACTGGG GAGCTATTGT TCGTCAGCAG TTGGGTCATA TTGAGGCCTG GGCAGAAAAA
    CAGGGTTTAG AATTGGCTGC TGATTGCCAC TTAAGCAGAA TAGTGCAGGC AACCACATTG
    CTCACCATGG ACAAGTACTC ACATGCTGAT GTTCCAAATA TTAACAGTAC TTGCTTTAAG
    TTGAATTCTC TACAGCTACA AGCTTTGTTG CAGAATTATC ACTGTGCTCC AGATGAACCG
    CTTATACCAA CAGAACTGAT AGAGAATGTA GTAACTGTTG CAGAAAATAC GGCTGATGAA
    CTTGCTCGCA GTGATGGCAG AGAAGTCCAA TTGGAAGAGG ATCCTGACTT ACAACTCCCT
    TTTCTTTTAC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAATGTTCC AAGTGGTTTA
    CAAGAATTTT TGGATCCCCT CTGTCAGAGA GGTTTTTGTA GGTTAATACC TCATACACGG
    TCACCAGGCA CATGGACAAT ATACTTTGAA GGCGCAGACT ATGAAAGCCA CCTGTCACAT
    GAGAATGCTG AGCTAGCTCA GCCTTTGAGG AAAGAACCTG AGATTATAAC TGTAACACTA
    AAAAAACAAA ATGGAATGGG CCTCAGCATT GTTGCTGCCA AGGGTGCTGG TCAAGACAAA
    CTTGGAATAT ATGTCAAGTC TGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGTCGTCTG
    GCAGCAGGGG ATCAGCTGCT CAGTGTGGAT GGTCGAAGCT TGGTGGGGCT GTCCCAGGAA
    AGGGCAGCAG AACTTATGAC GAGGACAGGT TCTGTTGTAA CACTGGAAGT AGCAAAACAA
    GGAGCAATAT ACCACGGTCT GGCAACCCTG CTAAATCAGC CATCTCCAAT GATGCAAAGA
    GTTTCTGACC GCCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGTTT TGAGCTGTAT
    AACAACTCCA CGCAAAATGG GTCACCTGAG AGCCCCCAGC TGCCATGGAC GGAGTACAGT
    GAACCAAAGA AGCTGCCAGG AGATGACAGA TTAATGAAGA ACAGAGCCGA TCATCGCTCC
    AGCCCCAACG TAGCAAATCA GCCTCCAAGC CCTGGGGGGA AGAATGCTTA TGCATCTGGA
    ACTACCACCA AAATAACCTC AGTCTCGACT GGAAATCTTT GTACAGAGGA GCAAACTCTG
    CCTCCACGAC CTGAAGCTTA TCCTATCCCA ACACAGACCT ATACCAGAGA ATATTTCACA
    TTCCCAGCTT CAAAATCCCA GGATCGAATG GGTCCAGCTC AGAGTCAGTG GCCAAATTAT
    GAAGAGAAGC CACAGATCCA GGCAGAAAGT AATCACTCAA TCAATACTAC AATGCAGCGC
    GTGGCTCGAT CCCAGGAAGA ACTCCGAGAT AAAGTTTTCC AGCCAGAGAG GAATCGTTTG
    GAAGTTGTTA TCCGGAAGGA CATAGAATAC ATTGATCGGA AGTCAGACAG CGATCAGTGG
    ATGAATTCAT CTGTGGATTC TAGCACATCT AGCCAAGAGC ACCTGAACCA TACATCCAAA
    CCTGTTTCAC CTGGTTCTTG CTTAACTAAA AGTGGACCTG GCCGTTGGAA AACTCCAGCT
    ACGAGCCAGC CAACTCCAGT GGCCGTTTCC CAGCCCATCA GAACAGACCT TCCTCCTCCG
    CCGCCACCTC CTCCGGTGCA TTATGCAGCT GAATTTGATG AACCACAAAT GGATTTGCCT
    CTGCCTCCCC CTCCTCCACC GAACCAAGTA GGAGCACAAG CATCTTCTCA GGTGGCTGCT
    GCTGAGCGTA AAAAACGAGA AGAGCATCAG AGGTGGTATG AAAAGGAAAA GGCACGTTTG
    GAAGAAGAAC GAGAAAGGAA ACGCCGTGAG CAAGAGAGAA AACTGGGCCA AATGCGTTCG
    CAGCCACTAA TTCCTCCCCA GCCTGTGGTT CCTCCTGTTT CCCTTCAGCA AGTGAAATCG
    GAAAAAACTT CAACTTTCCA GAGGTCTCAA GAAACAGTCA TTCGAGAGTT GCAGCCGCAA
    CAGCAGCCTC GGACTATTGA GCGGAGAGAT CTGCAGTACA TTACTATCAG CAAGGAAGAA
    TTGTCCTCTA GTGACAGCCT CTCTCCAGAT CCCTGGAAAC GAGATGCCAA GGAGAAACTG
    GAGAAGCAGC AGCAGATGCA CATTGTGGAC ATGCTGAGCA AAGAGATTCA GGATCTTCAG
    AACAAGCCTG ATCGTACAGC TGAAGAGAAC GACCGCCTGC GCAAACTCAT GCTAGAGTGG
    CAGTTCCAAA AGCGACTTCA GGAATCAAAA CAAAAGGATG AAGATGATGA TGAAGAAGAG
    GACGATGATG TGGATACTAT GCTTATCATG CAGCGGCTGG AGGCAGAAAG AAGAGCAAGG
    CAGACTGCTG TGCCAGCAAT CTCTGTTCTA GACTTGTTAC AGGATGAAGA ACGCAGACGA
    CAGCAACAGT TAGAAGAAAT GCGCAAGCGA GAAGCAGAAG ACAGGGCCAG GCAGGAAGAG
    GAGCGCCGAC AGCAAGAGGA GGAGCGAACC AGGAGAGAGG CAGAAGAAAA GAGGCGACAG
    GAAGAGGAGT ATTACAATCG CTTAGAGGCT GAAAGGCGCA GGCAGCACGA TGAAGCAGAA
    CGAAGATTGC TGGAACCAGA CGAGCCTGGT CTGTACAGAC CTCCACTTCC ACGGGAATAT
    GAACTCCCAT CCCCAACCCC TTCATCTAAC GCTCCTCCTC CCCCACCTCA GCGAAACACC
    TCTTACCTCA AAACACAGGT CCTCTCCCCT GACACGATTT ATACTGCCAA GTTTGTTGCA
    TACAATGATG ATGAGGAGGA GGAGGATTCC AATCTAGCAG GTCAGGATAA GTACTCCAGC
    ACAAGAAAAT CTTATGGTGA CTTTCCTCCT GCCACCCTTA AACCACAGCA GCCCTCCCGC
    CAGCCTCGCC CAGTTTCAGA TGGCCTCTTT CTTTCCAACT CATTCCAGTC ATCTCCAGTC
    AATGCCAACA GTACTGCTGC CAAAAAAAGC CAGCCCCCGC CCCCACCAAA CAAACCGAGT
    TTCATCCATG CCAGCAGCTC TAAAGGAATA AATTCATACA CTGGATCTTC GGGAGCAACG
    GTTGGGGCCC ATGAAGTCTA TCGGGATCCA AGAGACAAAC GATCTAAAAG TCAAGATACA
    GATTTACCTG GAGCACCAGA AAATCTGACG TTCAGGGAAC GCCAGCGACT TTTTTCACAG
    GGTCAAGATG TATCCAATAA AGTAAAAGCC TCTAGGAAAC TAATGGAACT GGAAAATGAG
    TTGAACACGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139831.1
    CDS2885..8659
    Translation

    Target ORF information:

    RefSeq Version XM_015284345.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284345.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAAGAA GATTAGATGT GGATGAGAAG CCTCTTGTTG TACAATTAAA CTGGAACAAA
    GATGATCGAG AAGGAAGGTT TGTTCTCAAG AATGAAAATG ATACGCTTCC TCCAAAGAAG
    GCTCAGAGTA ATGGCCCTGA AAAACAAGAA AAAGAAGGAG TAATACAGAA TTTCAAACGA
    ACTCTCTCAA AGAAAGAAAA GAAAGAGAAG AAGAGGCGTG AAAAAGAGGC ATTGCGACAA
    GCATCAGATA AAGATGATCG ACTTCTTCAT GGGGATGATA TTGAGAATTC TCGCCTTGCA
    GCTGAGGTTT ACAAAGATAT GCCTGAGACC AGCTTCACTC GCACAATATC TAATCCTGAG
    GTGGTTATGA AACGACGGCG GCAGCAAAAA CTAGAGAAGA GAATGCAGGA ATTTCGCAGT
    TCTGATGGCA GACCAGACTC AGGTGGGACG CTGAGAATTT ATGCGGACAG TTTAAAACCA
    AATATACCAT ACAAGACAAT CCTGTTGTCA ACTACAGATA CTGCAGACTT TGCAGTTATT
    GAAGCACTGG AGAAATATGG CTTGGAGAAG GAAAACCCCA AGGATTATTG TATTGCTCGT
    GTCATTCTGC CTCCTGGTGC TCAGCACTCT GATGATAAAG GTGCTAAAGA AACTATTCTA
    GATGATGATG AGTGTCCACT TCAGATATTC AGGGAATGGC CTAGTGATAA AGGAATACTA
    GTCTTTCAGT TGAAAAGGCG GCCTCCTGAT TATGTCCCAA AGAAATCCAA GAAAATAGCT
    GATGGAAAGC CATTAAAGGG GAAGGAAAGA GTTGACGGGT CTGGCTACGG CTCTTGCCTT
    CCTCCTGAGA AACTACCATA CCTGGTAGAA TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACGCTTA TGAAGATGGT TCTGACTCCA GAGATAAACC AAAGCTTTAT
    CGTCTACAAT TAAGTGTCAC TGAAGTTGGA ACTGAAAAAT TTGATGACAA TTCCATTCAG
    TTATTTGGTC CAGGAATTCA GCCTCATCAT TGTGATCTTA CTAATATGGA TGGAGTTGTT
    ACTGTAACCC CAAGAAACAT TGATGCGGAA ACGTATGTAG AAGGTCAGCG TATTTCAGAA
    ACCACTATGC TGCAAAGTGG AATGAAGGTT CAGTTTGGGT CATCTCATGT GTTTAAGTTT
    GTGGATCCCA CTCAGGACCA TATTATTACT AAAAGACCTA TTGATGCAAG CCTTATGGTC
    AAAGGTCCAA GGCATAAAGC TGGAGCTGTT CAGGAAACCA CGTTTGATCT GGAGGGAGAT
    GTTCACAGTG GAACAGCGTT ACCAGCGAGC AAGAGCACCA GCAGGCTTGA TGGTGACAGA
    ACATCATCAG CATCCAGCAC AGCTGAACGA GGAATGGTGA AGCCCATGAT TAGAATAGAA
    CAGCAGCAAG ATTATCGCAG ACAGGAAAGC AGACCTCAGG ATACCCATGG ACCAGAACTG
    ATCCTACCTG CCAGTATTGA ATTCAGGGAA AGCTCTGAAG ATGCCTTCTT ATCTGCCATT
    ATAAATTATA CAAATAGCTC GACAGTTCAT TTTAAACTGT CACCCACGTA TGTTTTGTAT
    ATGACATGTC GGTATGTATT GTCAAGCCAG TACAGACCTG ACATCACTCC TACTGAGCGT
    ACTCACAAAG TTATTGCAAT AGTCAACAAG ATGGTGAATA TGATGGAAGG AGTCATACAG
    GAGGTAGACC AGGTTGATCA GAAACAGAAG AATATTGCTG GGGCACTTGC CTTTTGGATG
    GCAAATGCGT CTGAATTGCT GAATTTTATA AAGCAAGACC GTGATCTGAG CCGAATCACT
    GTGGATGCTC AAGATGTTTT GGCGCACTTG GTTCAAATGG CATTCAAGTA CCTAGTTCAT
    TGTCTGCAGT CAGAGCTTAA TAACTACATG CCAGCATTTC TTGATGATCC AGAGGAAAAT
    AATCCACAGA GGCCTAAAAT AGATGATGTG CTGCACACAT TGACTGGAGC TATGTCCTTG
    TTGCGACGAT GTAGAGTGAA TGCTGCACTG ACTATACAGC TCTTCTCCCA GTTGTTTCAT
    TTTATCAACA TGTGGCTTTT TAACAGACTA GTTACGGCTC CAGATTCAGG GTTATGTTCC
    CACTACTGGG GAGCTATTGT TCGTCAGCAG TTGGGTCATA TTGAGGCCTG GGCAGAAAAA
    CAGGGTTTAG AATTGGCTGC TGATTGCCAC TTAAGCAGAA TAGTGCAGGC AACCACATTG
    CTCACCATGG ACAAGTACTC ACATGCTGAT GTTCCAAATA TTAACAGTAC TTGCTTTAAG
    TTGAATTCTC TACAGCTACA AGCTTTGTTG CAGAATTATC ACTGTGCTCC AGATGAACCG
    CTTATACCAA CAGAACTGAT AGAGAATGTA GTAACTGTTG CAGAAAATAC GGCTGATGAA
    CTTGCTCGCA GTGATGGCAG AGAAGTCCAA TTGGAAGAGG ATCCTGACTT ACAACTCCCT
    TTTCTTTTAC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAATGTTCC AAGTGGTTTA
    CAAGAATTTT TGGATCCCCT CTGTCAGAGA GGTTTTTGTA GGTTAATACC TCATACACGG
    TCACCAGGCA CATGGACAAT ATACTTTGAA GGCGCAGACT ATGAAAGCCA CCTGTCACAT
    GAGAATGCTG AGCTAGCTCA GCCTTTGAGG AAAGAACCTG AGATTATAAC TGTAACACTA
    AAAAAACAAA ATGGAATGGG CCTCAGCATT GTTGCTGCCA AGGGTGCTGG TCAAGACAAA
    CTTGGAATAT ATGTCAAGTC TGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGTCGTCTG
    GCAGCAGGGG ATCAGCTGCT CAGTGTGGAT GGTCGAAGCT TGGTGGGGCT GTCCCAGGAA
    AGGGCAGCAG AACTTATGAC GAGGACAGGT TCTGTTGTAA CACTGGAAGT AGCAAAACAA
    GGAGCAATAT ACCACGGTCT GGCAACCCTG CTAAATCAGC CATCTCCAAT GATGCAAAGA
    GTTTCTGACC GCCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGTTT TGAGCTGTAT
    AACAACTCCA CGCAAAATGG GTCACCTGAG AGCCCCCAGC TGCCATGGAC GGAGTACAGT
    GAACCAAAGA AGCTGCCAGG AGATGACAGA TTAATGAAGA ACAGAGCCGA TCATCGCTCC
    AGCCCCAACG TAGCAAATCA GCCTCCAAGC CCTGGGGGGA AGAATGCTTA TGCATCTGGA
    ACTACCACCA AAATAACCTC AGTCTCGACT GGAAATCTTT GTACAGAGGA GCAAACTCTG
    CCTCCACGAC CTGAAGCTTA TCCTATCCCA ACACAGACCT ATACCAGAGA ATATTTCACA
    TTCCCAGCTT CAAAATCCCA GGATCGAATG GGTCCAGCTC AGAGTCAGTG GCCAAATTAT
    GAAGAGAAGC CACAGATCCA GGCAGAAAGT AATCACTCAA TCAATACTAC AATGCAGCGC
    GTGGCTCGAT CCCAGGAAGA ACTCCGAGAT AAAGTTTTCC AGCCAGAGAG GAATCGTTTG
    GAAGTTGTTA TCCGGAAGGA CATAGAATAC ATTGATCGGA AGTCAGACAG CGATCAGTGG
    ATGAATTCAT CTGTGGATTC TAGCACATCT AGCCAAGAGC ACCTGAACCA TACATCCAAA
    CCTGTTTCAC CTGGTTCTTG CTTAACTAAA AGTGGACCTG GCCGTTGGAA AACTCCAGCT
    ACGAGCCAGC CAACTCCAGT GGCCGTTTCC CAGCCCATCA GAACAGACCT TCCTCCTCCG
    CCGCCACCTC CTCCGGTGCA TTATGCAGCT GAATTTGATG AACCACAAAT GGATTTGCCT
    CTGCCTCCCC CTCCTCCACC GAACCAAGTA GGAGCACAAG CATCTTCTCA GGTGGCTGCT
    GCTGAGCGTA AAAAACGAGA AGAGCATCAG AGGTGGTATG AAAAGGAAAA GGCACGTTTG
    GAAGAAGAAC GAGAAAGGAA ACGCCGTGAG CAAGAGAGAA AACTGGGCCA AATGCGTTCG
    CAGCCACTAA TTCCTCCCCA GCCTGTGGTT CCTCCTGTTT CCCTTCAGCA AGTGAAATCG
    GAAAAAACTT CAACTTTCCA GAGGTCTCAA GAAACAGTCA TTCGAGAGTT GCAGCCGCAA
    CAGCAGCCTC GGACTATTGA GCGGAGAGAT CTGCAGTACA TTACTATCAG CAAGGAAGAA
    TTGTCCTCTA GTGACAGCCT CTCTCCAGAT CCCTGGAAAC GAGATGCCAA GGAGAAACTG
    GAGAAGCAGC AGCAGATGCA CATTGTGGAC ATGCTGAGCA AAGAGATTCA GGATCTTCAG
    AACAAGCCTG ATCGTACAGC TGAAGAGAAC GACCGCCTGC GCAAACTCAT GCTAGAGTGG
    CAGTTCCAAA AGCGACTTCA GGAATCAAAA CAAAAGGATG AAGATGATGA TGAAGAAGAG
    GACGATGATG TGGATACTAT GCTTATCATG CAGCGGCTGG AGGCAGAAAG AAGAGCAAGG
    CAGACTGCTG TGCCAGCAAT CTCTGTTCTA GACTTGTTAC AGGATGAAGA ACGCAGACGA
    CAGCAACAGT TAGAAGAAAT GCGCAAGCGA GAAGCAGAAG ACAGGGCCAG GCAGGAAGAG
    GAGCGCCGAC AGCAAGAGGA GGAGCGAACC AGGAGAGAGG CAGAAGAAAA GAGGCGACAG
    GAAGAGGAGT ATTACAATCG CTTAGAGGCT GAAAGGCGCA GGCAGCACGA TGAAGCAGAA
    CGAAGATTGC TGGAACCAGA CGAGCCTGGT CTGTACAGAC CTCCACTTCC ACGGGAATAT
    GAACTCCCAT CCCCAACCCC TTCATCTAAC GCTCCTCCTC CCCCACCTCA GCGAAACACC
    TCTTACCTCA AAACACAGGT CCTCTCCCCT GACACGATTT ATACTGCCAA GTTTGTTGCA
    TACAATGATG ATGAGGAGGA GGAGGATTCC AATCTAGCAG GTCAGGATAA GTACTCCAGC
    ACAAGAAAAT CTTATGGTGA CTTTCCTCCT GCCACCCTTA AACCACAGCA GCCCTCCCGC
    CAGCCTCGCC CAGTTTCAGA TGGCCTCTTT CTTTCCAACT CATTCCAGTC ATCTCCAGTC
    AATGCCAACA GTACTGCTGC CAAAAAAAGC CAGCCCCCGC CCCCACCAAA CAAACCGAGT
    TTCATCCATG CCAGCAGCTC TAAAGGAATA AATTCATACA CTGGATCTTC GGGAGCAACG
    GTTGGGGCCC ATGAAGTCTA TCGGGATCCA AGAGACAAAC GATCTAAAAG TCAAGATACA
    GATTTACCTG GAGCACCAGA AAATCTGACG TTCAGGGAAC GCCAGCGACT TTTTTCACAG
    GGTCAAGATG TATCCAATAA AGTAAAAGCC TCTAGGAAAC TAATGGAACT GGAAAATGAG
    TTGAACACGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33796
    Clone ID Related Accession (Same CDS sequence) XM_015284346.1 , XM_015284346.2
    Accession Version XM_015284346.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGG ATGAAGAACG CAGACGACAG
    CAACAGTTAG AAGAAATGCG CAAGCGAGAA GCAGAAGACA GGGCCAGGCA GGAAGAGGAG
    CGCCGACAGC AAGAGGAGGA GCGAACCAGG AGAGAGGCAG AAGAAAAGAG GCGACAGGAA
    GAGGAGTATT ACAATCGCTT AGAGGCTGAA AGGCGCAGGC AGCACGATGA AGCAGAACGA
    AGATTGCTGG AACCAGACGA GCCTGGTCTG TACAGACCTC CACTTCCACG GGAATATGAA
    CTCCCATCCC CAACCCCTTC ATCTAACGCT CCTCCTCCCC CACCTCAGCG AAACACCTCT
    TACCTCAAAA CACAGGTCCT CTCCCCTGAC ACGATTTATA CTGCCAAGTT TGTTGCATAC
    AATGATGATG AGGAGGAGGA GGATTCCAAT CTAGCAGGTC AGGATAAGTA CTCCAGCACA
    AGAAAATCTT ATGGTGACTT TCCTCCTGCC ACCCTTAAAC CACAGCAGCC CTCCCGCCAG
    CCTCGCCCAG TTTCAGATGG CCTCTTTCTT TCCAACTCAT TCCAGTCATC TCCAGTCAAT
    GCCAACAGTA CTGCTGCCAA AAAAAGCCAG CCCCCGCCCC CACCAAACAA ACCGAGTTTC
    ATCCATGCCA GCAGCTCTAA AGGAATAAAT TCATACACTG GATCTTCGGG AGCAACGGTT
    GGGGCCCATG AAGTCTATCG GGATCCAAGA GACAAACGAT CTAAAAGTCA AGATACAGAT
    TTACCTGGAG CACCAGAAAA TCTGACGTTC AGGGAACGCC AGCGACTTTT TTCACAGGGT
    CAAGATGTAT CCAATAAAGT AAAAGCCTCT AGGAAACTAA TGGAACTGGA AAATGAGTTG
    AACACGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139832.1
    CDS33..5804
    Translation

    Target ORF information:

    RefSeq Version XM_015284346.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284346.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGG ATGAAGAACG CAGACGACAG
    CAACAGTTAG AAGAAATGCG CAAGCGAGAA GCAGAAGACA GGGCCAGGCA GGAAGAGGAG
    CGCCGACAGC AAGAGGAGGA GCGAACCAGG AGAGAGGCAG AAGAAAAGAG GCGACAGGAA
    GAGGAGTATT ACAATCGCTT AGAGGCTGAA AGGCGCAGGC AGCACGATGA AGCAGAACGA
    AGATTGCTGG AACCAGACGA GCCTGGTCTG TACAGACCTC CACTTCCACG GGAATATGAA
    CTCCCATCCC CAACCCCTTC ATCTAACGCT CCTCCTCCCC CACCTCAGCG AAACACCTCT
    TACCTCAAAA CACAGGTCCT CTCCCCTGAC ACGATTTATA CTGCCAAGTT TGTTGCATAC
    AATGATGATG AGGAGGAGGA GGATTCCAAT CTAGCAGGTC AGGATAAGTA CTCCAGCACA
    AGAAAATCTT ATGGTGACTT TCCTCCTGCC ACCCTTAAAC CACAGCAGCC CTCCCGCCAG
    CCTCGCCCAG TTTCAGATGG CCTCTTTCTT TCCAACTCAT TCCAGTCATC TCCAGTCAAT
    GCCAACAGTA CTGCTGCCAA AAAAAGCCAG CCCCCGCCCC CACCAAACAA ACCGAGTTTC
    ATCCATGCCA GCAGCTCTAA AGGAATAAAT TCATACACTG GATCTTCGGG AGCAACGGTT
    GGGGCCCATG AAGTCTATCG GGATCCAAGA GACAAACGAT CTAAAAGTCA AGATACAGAT
    TTACCTGGAG CACCAGAAAA TCTGACGTTC AGGGAACGCC AGCGACTTTT TTCACAGGGT
    CAAGATGTAT CCAATAAAGT AAAAGCCTCT AGGAAACTAA TGGAACTGGA AAATGAGTTG
    AACACGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33796
    Clone ID Related Accession (Same CDS sequence) XM_015284346.1 , XM_015284346.2
    Accession Version XM_015284346.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGG ATGAAGAACG CAGACGACAG
    CAACAGTTAG AAGAAATGCG CAAGCGAGAA GCAGAAGACA GGGCCAGGCA GGAAGAGGAG
    CGCCGACAGC AAGAGGAGGA GCGAACCAGG AGAGAGGCAG AAGAAAAGAG GCGACAGGAA
    GAGGAGTATT ACAATCGCTT AGAGGCTGAA AGGCGCAGGC AGCACGATGA AGCAGAACGA
    AGATTGCTGG AACCAGACGA GCCTGGTCTG TACAGACCTC CACTTCCACG GGAATATGAA
    CTCCCATCCC CAACCCCTTC ATCTAACGCT CCTCCTCCCC CACCTCAGCG AAACACCTCT
    TACCTCAAAA CACAGGTCCT CTCCCCTGAC ACGATTTATA CTGCCAAGTT TGTTGCATAC
    AATGATGATG AGGAGGAGGA GGATTCCAAT CTAGCAGGTC AGGATAAGTA CTCCAGCACA
    AGAAAATCTT ATGGTGACTT TCCTCCTGCC ACCCTTAAAC CACAGCAGCC CTCCCGCCAG
    CCTCGCCCAG TTTCAGATGG CCTCTTTCTT TCCAACTCAT TCCAGTCATC TCCAGTCAAT
    GCCAACAGTA CTGCTGCCAA AAAAAGCCAG CCCCCGCCCC CACCAAACAA ACCGAGTTTC
    ATCCATGCCA GCAGCTCTAA AGGAATAAAT TCATACACTG GATCTTCGGG AGCAACGGTT
    GGGGCCCATG AAGTCTATCG GGATCCAAGA GACAAACGAT CTAAAAGTCA AGATACAGAT
    TTACCTGGAG CACCAGAAAA TCTGACGTTC AGGGAACGCC AGCGACTTTT TTCACAGGGT
    CAAGATGTAT CCAATAAAGT AAAAGCCTCT AGGAAACTAA TGGAACTGGA AAATGAGTTG
    AACACGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139832.1
    CDS2885..8656
    Translation

    Target ORF information:

    RefSeq Version XM_015284346.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284346.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGG ATGAAGAACG CAGACGACAG
    CAACAGTTAG AAGAAATGCG CAAGCGAGAA GCAGAAGACA GGGCCAGGCA GGAAGAGGAG
    CGCCGACAGC AAGAGGAGGA GCGAACCAGG AGAGAGGCAG AAGAAAAGAG GCGACAGGAA
    GAGGAGTATT ACAATCGCTT AGAGGCTGAA AGGCGCAGGC AGCACGATGA AGCAGAACGA
    AGATTGCTGG AACCAGACGA GCCTGGTCTG TACAGACCTC CACTTCCACG GGAATATGAA
    CTCCCATCCC CAACCCCTTC ATCTAACGCT CCTCCTCCCC CACCTCAGCG AAACACCTCT
    TACCTCAAAA CACAGGTCCT CTCCCCTGAC ACGATTTATA CTGCCAAGTT TGTTGCATAC
    AATGATGATG AGGAGGAGGA GGATTCCAAT CTAGCAGGTC AGGATAAGTA CTCCAGCACA
    AGAAAATCTT ATGGTGACTT TCCTCCTGCC ACCCTTAAAC CACAGCAGCC CTCCCGCCAG
    CCTCGCCCAG TTTCAGATGG CCTCTTTCTT TCCAACTCAT TCCAGTCATC TCCAGTCAAT
    GCCAACAGTA CTGCTGCCAA AAAAAGCCAG CCCCCGCCCC CACCAAACAA ACCGAGTTTC
    ATCCATGCCA GCAGCTCTAA AGGAATAAAT TCATACACTG GATCTTCGGG AGCAACGGTT
    GGGGCCCATG AAGTCTATCG GGATCCAAGA GACAAACGAT CTAAAAGTCA AGATACAGAT
    TTACCTGGAG CACCAGAAAA TCTGACGTTC AGGGAACGCC AGCGACTTTT TTCACAGGGT
    CAAGATGTAT CCAATAAAGT AAAAGCCTCT AGGAAACTAA TGGAACTGGA AAATGAGTTG
    AACACGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33797
    Clone ID Related Accession (Same CDS sequence) XM_015284347.1 , XM_015284347.2
    Accession Version XM_015284347.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGGTAAAC CCCGACCAAA GAGTGAAGGT TTTGAGCTGT ATAACAACTC CACGCAAAAT
    GGGTCACCTG AGAGCCCCCA GCTGCCATGG ACGGAGTACA GTGAACCAAA GAAGCTGCCA
    GGAGATGACA GATTAATGAA GAACAGAGCC GATCATCGCT CCAGCCCCAA CGTAGCAAAT
    CAGCCTCCAA GCCCTGGGGG GAAGAATGCT TATGCATCTG GAACTACCAC CAAAATAACC
    TCAGTCTCGA CTGGAAATCT TTGTACAGAG GAGCAAACTC TGCCTCCACG ACCTGAAGCT
    TATCCTATCC CAACACAGAC CTATACCAGA GAATATTTCA CATTCCCAGC TTCAAAATCC
    CAGGATCGAA TGGGTCCAGC TCAGAGTCAG TGGCCAAATT ATGAAGAGAA GCCACAGATC
    CAGGCAGAAA GTAATCACTC AATCAATACT ACAATGCAGC GCGTGGCTCG ATCCCAGGAA
    GAACTCCGAG ATAAAGTTTT CCAGCCAGAG AGGAATCGTT TGGAAGTTGT TATCCGGAAG
    GACATAGAAT ACATTGATCG GAAGTCAGAC AGCGATCAGT GGATGAATTC ATCTGTGGAT
    TCTAGCACAT CTAGCCAAGA GCACCTGAAC CATACATCCA AACCTGTTTC ACCTGGTTCT
    TGCTTAACTA AAAGTGGACC TGGCCGTTGG AAAACTCCAG CTACGAGCCA GCCAACTCCA
    GTGGCCGTTT CCCAGCCCAT CAGAACAGAC CTTCCTCCTC CGCCGCCACC TCCTCCGGTG
    CATTATGCAG CTGAATTTGA TGAACCACAA ATGGATTTGC CTCTGCCTCC CCCTCCTCCA
    CCGAACCAAG TAGGAGCACA AGCATCTTCT CAGGTGGCTG CTGCTGAGCG TAAAAAACGA
    GAAGAGCATC AGAGGTGGTA TGAAAAGGAA AAGGCACGTT TGGAAGAAGA ACGAGAAAGG
    AAACGCCGTG AGCAAGAGAG AAAACTGGGC CAAATGCGTT CGCAGCCACT AATTCCTCCC
    CAGCCTGTGG TTCCTCCTGT TTCCCTTCAG CAAGTGAAAT CGGAAAAAAC TTCAACTTTC
    CAGAGGTCTC AAGAAACAGT CATTCGAGAG TTGCAGCCGC AACAGCAGCC TCGGACTATT
    GAGCGGAGAG ATCTGCAGTA CATTACTATC AGCAAGGAAG AATTGTCCTC TAGTGACAGC
    CTCTCTCCAG ATCCCTGGAA ACGAGATGCC AAGGAGAAAC TGGAGAAGCA GCAGCAGATG
    CACATTGTGG ACATGCTGAG CAAAGAGATT CAGGATCTTC AGAACAAGCC TGATCGTACA
    GCTGAAGAGA ACGACCGCCT GCGCAAACTC ATGCTAGAGT GGCAGTTCCA AAAGCGACTT
    CAGGAATCAA AACAAAAGGA TGAAGATGAT GATGAAGAAG AGGACGATGA TGTGGATACT
    ATGCTTATCA TGCAGCGGCT GGAGGCAGAA AGAAGAGCAA GGCAGACTGC TGTGCCAGCA
    ATCTCTGTTC TAGACTTGTT ACAGGATGAA GAACGCAGAC GACAGCAACA GTTAGAAGAA
    ATGCGCAAGC GAGAAGCAGA AGACAGGGCC AGGCAGGAAG AGGAGCGCCG ACAGCAAGAG
    GAGGAGCGAA CCAGGAGAGA GGCAGAAGAA AAGAGGCGAC AGGAAGAGGA GTATTACAAT
    CGCTTAGAGG CTGAAAGGCG CAGGCAGCAC GATGAAGCAG AACGAAGATT GCTGGAACCA
    GACGAGCCTG GTCTGTACAG ACCTCCACTT CCACGGGAAT ATGAACTCCC ATCCCCAACC
    CCTTCATCTA ACGCTCCTCC TCCCCCACCT CAGCGAAACA CCTCTTACCT CAAAACACAG
    GTCCTCTCCC CTGACACGAT TTATACTGCC AAGTTTGTTG CATACAATGA TGATGAGGAG
    GAGGAGGATT CCAATCTAGC AGGTCAGGAT AAGTACTCCA GCACAAGAAA ATCTTATGGT
    GACTTTCCTC CTGCCACCCT TAAACCACAG CAGCCCTCCC GCCAGCCTCG CCCAGTTTCA
    GATGGCCTCT TTCTTTCCAA CTCATTCCAG TCATCTCCAG TCAATGCCAA CAGTACTGCT
    GCCAAAAAAA GCCAGCCCCC GCCCCCACCA AACAAACCGA GTTTCATCCA TGCCAGCAGC
    TCTAAAGGAA TAAATTCATA CACTGGATCT TCGGGAGCAA CGGTTGGGGC CCATGAAGTC
    TATCGGGATC CAAGAGACAA ACGATCTAAA AGTCAAGATA CAGATTTACC TGGAGCACCA
    GAAAATCTGA CGTTCAGGGA ACGCCAGCGA CTTTTTTCAC AGGGTCAAGA TGTATCCAAT
    AAAGTAAAAG CCTCTAGGAA ACTAATGGAA CTGGAAAATG AGTTGAACAC GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139833.1
    CDS33..5789
    Translation

    Target ORF information:

    RefSeq Version XM_015284347.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284347.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGGTAAAC CCCGACCAAA GAGTGAAGGT TTTGAGCTGT ATAACAACTC CACGCAAAAT
    GGGTCACCTG AGAGCCCCCA GCTGCCATGG ACGGAGTACA GTGAACCAAA GAAGCTGCCA
    GGAGATGACA GATTAATGAA GAACAGAGCC GATCATCGCT CCAGCCCCAA CGTAGCAAAT
    CAGCCTCCAA GCCCTGGGGG GAAGAATGCT TATGCATCTG GAACTACCAC CAAAATAACC
    TCAGTCTCGA CTGGAAATCT TTGTACAGAG GAGCAAACTC TGCCTCCACG ACCTGAAGCT
    TATCCTATCC CAACACAGAC CTATACCAGA GAATATTTCA CATTCCCAGC TTCAAAATCC
    CAGGATCGAA TGGGTCCAGC TCAGAGTCAG TGGCCAAATT ATGAAGAGAA GCCACAGATC
    CAGGCAGAAA GTAATCACTC AATCAATACT ACAATGCAGC GCGTGGCTCG ATCCCAGGAA
    GAACTCCGAG ATAAAGTTTT CCAGCCAGAG AGGAATCGTT TGGAAGTTGT TATCCGGAAG
    GACATAGAAT ACATTGATCG GAAGTCAGAC AGCGATCAGT GGATGAATTC ATCTGTGGAT
    TCTAGCACAT CTAGCCAAGA GCACCTGAAC CATACATCCA AACCTGTTTC ACCTGGTTCT
    TGCTTAACTA AAAGTGGACC TGGCCGTTGG AAAACTCCAG CTACGAGCCA GCCAACTCCA
    GTGGCCGTTT CCCAGCCCAT CAGAACAGAC CTTCCTCCTC CGCCGCCACC TCCTCCGGTG
    CATTATGCAG CTGAATTTGA TGAACCACAA ATGGATTTGC CTCTGCCTCC CCCTCCTCCA
    CCGAACCAAG TAGGAGCACA AGCATCTTCT CAGGTGGCTG CTGCTGAGCG TAAAAAACGA
    GAAGAGCATC AGAGGTGGTA TGAAAAGGAA AAGGCACGTT TGGAAGAAGA ACGAGAAAGG
    AAACGCCGTG AGCAAGAGAG AAAACTGGGC CAAATGCGTT CGCAGCCACT AATTCCTCCC
    CAGCCTGTGG TTCCTCCTGT TTCCCTTCAG CAAGTGAAAT CGGAAAAAAC TTCAACTTTC
    CAGAGGTCTC AAGAAACAGT CATTCGAGAG TTGCAGCCGC AACAGCAGCC TCGGACTATT
    GAGCGGAGAG ATCTGCAGTA CATTACTATC AGCAAGGAAG AATTGTCCTC TAGTGACAGC
    CTCTCTCCAG ATCCCTGGAA ACGAGATGCC AAGGAGAAAC TGGAGAAGCA GCAGCAGATG
    CACATTGTGG ACATGCTGAG CAAAGAGATT CAGGATCTTC AGAACAAGCC TGATCGTACA
    GCTGAAGAGA ACGACCGCCT GCGCAAACTC ATGCTAGAGT GGCAGTTCCA AAAGCGACTT
    CAGGAATCAA AACAAAAGGA TGAAGATGAT GATGAAGAAG AGGACGATGA TGTGGATACT
    ATGCTTATCA TGCAGCGGCT GGAGGCAGAA AGAAGAGCAA GGCAGACTGC TGTGCCAGCA
    ATCTCTGTTC TAGACTTGTT ACAGGATGAA GAACGCAGAC GACAGCAACA GTTAGAAGAA
    ATGCGCAAGC GAGAAGCAGA AGACAGGGCC AGGCAGGAAG AGGAGCGCCG ACAGCAAGAG
    GAGGAGCGAA CCAGGAGAGA GGCAGAAGAA AAGAGGCGAC AGGAAGAGGA GTATTACAAT
    CGCTTAGAGG CTGAAAGGCG CAGGCAGCAC GATGAAGCAG AACGAAGATT GCTGGAACCA
    GACGAGCCTG GTCTGTACAG ACCTCCACTT CCACGGGAAT ATGAACTCCC ATCCCCAACC
    CCTTCATCTA ACGCTCCTCC TCCCCCACCT CAGCGAAACA CCTCTTACCT CAAAACACAG
    GTCCTCTCCC CTGACACGAT TTATACTGCC AAGTTTGTTG CATACAATGA TGATGAGGAG
    GAGGAGGATT CCAATCTAGC AGGTCAGGAT AAGTACTCCA GCACAAGAAA ATCTTATGGT
    GACTTTCCTC CTGCCACCCT TAAACCACAG CAGCCCTCCC GCCAGCCTCG CCCAGTTTCA
    GATGGCCTCT TTCTTTCCAA CTCATTCCAG TCATCTCCAG TCAATGCCAA CAGTACTGCT
    GCCAAAAAAA GCCAGCCCCC GCCCCCACCA AACAAACCGA GTTTCATCCA TGCCAGCAGC
    TCTAAAGGAA TAAATTCATA CACTGGATCT TCGGGAGCAA CGGTTGGGGC CCATGAAGTC
    TATCGGGATC CAAGAGACAA ACGATCTAAA AGTCAAGATA CAGATTTACC TGGAGCACCA
    GAAAATCTGA CGTTCAGGGA ACGCCAGCGA CTTTTTTCAC AGGGTCAAGA TGTATCCAAT
    AAAGTAAAAG CCTCTAGGAA ACTAATGGAA CTGGAAAATG AGTTGAACAC GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33797
    Clone ID Related Accession (Same CDS sequence) XM_015284347.1 , XM_015284347.2
    Accession Version XM_015284347.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284347.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGGTAAAC CCCGACCAAA GAGTGAAGGT TTTGAGCTGT ATAACAACTC CACGCAAAAT
    GGGTCACCTG AGAGCCCCCA GCTGCCATGG ACGGAGTACA GTGAACCAAA GAAGCTGCCA
    GGAGATGACA GATTAATGAA GAACAGAGCC GATCATCGCT CCAGCCCCAA CGTAGCAAAT
    CAGCCTCCAA GCCCTGGGGG GAAGAATGCT TATGCATCTG GAACTACCAC CAAAATAACC
    TCAGTCTCGA CTGGAAATCT TTGTACAGAG GAGCAAACTC TGCCTCCACG ACCTGAAGCT
    TATCCTATCC CAACACAGAC CTATACCAGA GAATATTTCA CATTCCCAGC TTCAAAATCC
    CAGGATCGAA TGGGTCCAGC TCAGAGTCAG TGGCCAAATT ATGAAGAGAA GCCACAGATC
    CAGGCAGAAA GTAATCACTC AATCAATACT ACAATGCAGC GCGTGGCTCG ATCCCAGGAA
    GAACTCCGAG ATAAAGTTTT CCAGCCAGAG AGGAATCGTT TGGAAGTTGT TATCCGGAAG
    GACATAGAAT ACATTGATCG GAAGTCAGAC AGCGATCAGT GGATGAATTC ATCTGTGGAT
    TCTAGCACAT CTAGCCAAGA GCACCTGAAC CATACATCCA AACCTGTTTC ACCTGGTTCT
    TGCTTAACTA AAAGTGGACC TGGCCGTTGG AAAACTCCAG CTACGAGCCA GCCAACTCCA
    GTGGCCGTTT CCCAGCCCAT CAGAACAGAC CTTCCTCCTC CGCCGCCACC TCCTCCGGTG
    CATTATGCAG CTGAATTTGA TGAACCACAA ATGGATTTGC CTCTGCCTCC CCCTCCTCCA
    CCGAACCAAG TAGGAGCACA AGCATCTTCT CAGGTGGCTG CTGCTGAGCG TAAAAAACGA
    GAAGAGCATC AGAGGTGGTA TGAAAAGGAA AAGGCACGTT TGGAAGAAGA ACGAGAAAGG
    AAACGCCGTG AGCAAGAGAG AAAACTGGGC CAAATGCGTT CGCAGCCACT AATTCCTCCC
    CAGCCTGTGG TTCCTCCTGT TTCCCTTCAG CAAGTGAAAT CGGAAAAAAC TTCAACTTTC
    CAGAGGTCTC AAGAAACAGT CATTCGAGAG TTGCAGCCGC AACAGCAGCC TCGGACTATT
    GAGCGGAGAG ATCTGCAGTA CATTACTATC AGCAAGGAAG AATTGTCCTC TAGTGACAGC
    CTCTCTCCAG ATCCCTGGAA ACGAGATGCC AAGGAGAAAC TGGAGAAGCA GCAGCAGATG
    CACATTGTGG ACATGCTGAG CAAAGAGATT CAGGATCTTC AGAACAAGCC TGATCGTACA
    GCTGAAGAGA ACGACCGCCT GCGCAAACTC ATGCTAGAGT GGCAGTTCCA AAAGCGACTT
    CAGGAATCAA AACAAAAGGA TGAAGATGAT GATGAAGAAG AGGACGATGA TGTGGATACT
    ATGCTTATCA TGCAGCGGCT GGAGGCAGAA AGAAGAGCAA GGCAGACTGC TGTGCCAGCA
    ATCTCTGTTC TAGACTTGTT ACAGGATGAA GAACGCAGAC GACAGCAACA GTTAGAAGAA
    ATGCGCAAGC GAGAAGCAGA AGACAGGGCC AGGCAGGAAG AGGAGCGCCG ACAGCAAGAG
    GAGGAGCGAA CCAGGAGAGA GGCAGAAGAA AAGAGGCGAC AGGAAGAGGA GTATTACAAT
    CGCTTAGAGG CTGAAAGGCG CAGGCAGCAC GATGAAGCAG AACGAAGATT GCTGGAACCA
    GACGAGCCTG GTCTGTACAG ACCTCCACTT CCACGGGAAT ATGAACTCCC ATCCCCAACC
    CCTTCATCTA ACGCTCCTCC TCCCCCACCT CAGCGAAACA CCTCTTACCT CAAAACACAG
    GTCCTCTCCC CTGACACGAT TTATACTGCC AAGTTTGTTG CATACAATGA TGATGAGGAG
    GAGGAGGATT CCAATCTAGC AGGTCAGGAT AAGTACTCCA GCACAAGAAA ATCTTATGGT
    GACTTTCCTC CTGCCACCCT TAAACCACAG CAGCCCTCCC GCCAGCCTCG CCCAGTTTCA
    GATGGCCTCT TTCTTTCCAA CTCATTCCAG TCATCTCCAG TCAATGCCAA CAGTACTGCT
    GCCAAAAAAA GCCAGCCCCC GCCCCCACCA AACAAACCGA GTTTCATCCA TGCCAGCAGC
    TCTAAAGGAA TAAATTCATA CACTGGATCT TCGGGAGCAA CGGTTGGGGC CCATGAAGTC
    TATCGGGATC CAAGAGACAA ACGATCTAAA AGTCAAGATA CAGATTTACC TGGAGCACCA
    GAAAATCTGA CGTTCAGGGA ACGCCAGCGA CTTTTTTCAC AGGGTCAAGA TGTATCCAAT
    AAAGTAAAAG CCTCTAGGAA ACTAATGGAA CTGGAAAATG AGTTGAACAC GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139833.1
    CDS2885..8641
    Translation

    Target ORF information:

    RefSeq Version XM_015284347.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284347.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGGTAAAC CCCGACCAAA GAGTGAAGGT TTTGAGCTGT ATAACAACTC CACGCAAAAT
    GGGTCACCTG AGAGCCCCCA GCTGCCATGG ACGGAGTACA GTGAACCAAA GAAGCTGCCA
    GGAGATGACA GATTAATGAA GAACAGAGCC GATCATCGCT CCAGCCCCAA CGTAGCAAAT
    CAGCCTCCAA GCCCTGGGGG GAAGAATGCT TATGCATCTG GAACTACCAC CAAAATAACC
    TCAGTCTCGA CTGGAAATCT TTGTACAGAG GAGCAAACTC TGCCTCCACG ACCTGAAGCT
    TATCCTATCC CAACACAGAC CTATACCAGA GAATATTTCA CATTCCCAGC TTCAAAATCC
    CAGGATCGAA TGGGTCCAGC TCAGAGTCAG TGGCCAAATT ATGAAGAGAA GCCACAGATC
    CAGGCAGAAA GTAATCACTC AATCAATACT ACAATGCAGC GCGTGGCTCG ATCCCAGGAA
    GAACTCCGAG ATAAAGTTTT CCAGCCAGAG AGGAATCGTT TGGAAGTTGT TATCCGGAAG
    GACATAGAAT ACATTGATCG GAAGTCAGAC AGCGATCAGT GGATGAATTC ATCTGTGGAT
    TCTAGCACAT CTAGCCAAGA GCACCTGAAC CATACATCCA AACCTGTTTC ACCTGGTTCT
    TGCTTAACTA AAAGTGGACC TGGCCGTTGG AAAACTCCAG CTACGAGCCA GCCAACTCCA
    GTGGCCGTTT CCCAGCCCAT CAGAACAGAC CTTCCTCCTC CGCCGCCACC TCCTCCGGTG
    CATTATGCAG CTGAATTTGA TGAACCACAA ATGGATTTGC CTCTGCCTCC CCCTCCTCCA
    CCGAACCAAG TAGGAGCACA AGCATCTTCT CAGGTGGCTG CTGCTGAGCG TAAAAAACGA
    GAAGAGCATC AGAGGTGGTA TGAAAAGGAA AAGGCACGTT TGGAAGAAGA ACGAGAAAGG
    AAACGCCGTG AGCAAGAGAG AAAACTGGGC CAAATGCGTT CGCAGCCACT AATTCCTCCC
    CAGCCTGTGG TTCCTCCTGT TTCCCTTCAG CAAGTGAAAT CGGAAAAAAC TTCAACTTTC
    CAGAGGTCTC AAGAAACAGT CATTCGAGAG TTGCAGCCGC AACAGCAGCC TCGGACTATT
    GAGCGGAGAG ATCTGCAGTA CATTACTATC AGCAAGGAAG AATTGTCCTC TAGTGACAGC
    CTCTCTCCAG ATCCCTGGAA ACGAGATGCC AAGGAGAAAC TGGAGAAGCA GCAGCAGATG
    CACATTGTGG ACATGCTGAG CAAAGAGATT CAGGATCTTC AGAACAAGCC TGATCGTACA
    GCTGAAGAGA ACGACCGCCT GCGCAAACTC ATGCTAGAGT GGCAGTTCCA AAAGCGACTT
    CAGGAATCAA AACAAAAGGA TGAAGATGAT GATGAAGAAG AGGACGATGA TGTGGATACT
    ATGCTTATCA TGCAGCGGCT GGAGGCAGAA AGAAGAGCAA GGCAGACTGC TGTGCCAGCA
    ATCTCTGTTC TAGACTTGTT ACAGGATGAA GAACGCAGAC GACAGCAACA GTTAGAAGAA
    ATGCGCAAGC GAGAAGCAGA AGACAGGGCC AGGCAGGAAG AGGAGCGCCG ACAGCAAGAG
    GAGGAGCGAA CCAGGAGAGA GGCAGAAGAA AAGAGGCGAC AGGAAGAGGA GTATTACAAT
    CGCTTAGAGG CTGAAAGGCG CAGGCAGCAC GATGAAGCAG AACGAAGATT GCTGGAACCA
    GACGAGCCTG GTCTGTACAG ACCTCCACTT CCACGGGAAT ATGAACTCCC ATCCCCAACC
    CCTTCATCTA ACGCTCCTCC TCCCCCACCT CAGCGAAACA CCTCTTACCT CAAAACACAG
    GTCCTCTCCC CTGACACGAT TTATACTGCC AAGTTTGTTG CATACAATGA TGATGAGGAG
    GAGGAGGATT CCAATCTAGC AGGTCAGGAT AAGTACTCCA GCACAAGAAA ATCTTATGGT
    GACTTTCCTC CTGCCACCCT TAAACCACAG CAGCCCTCCC GCCAGCCTCG CCCAGTTTCA
    GATGGCCTCT TTCTTTCCAA CTCATTCCAG TCATCTCCAG TCAATGCCAA CAGTACTGCT
    GCCAAAAAAA GCCAGCCCCC GCCCCCACCA AACAAACCGA GTTTCATCCA TGCCAGCAGC
    TCTAAAGGAA TAAATTCATA CACTGGATCT TCGGGAGCAA CGGTTGGGGC CCATGAAGTC
    TATCGGGATC CAAGAGACAA ACGATCTAAA AGTCAAGATA CAGATTTACC TGGAGCACCA
    GAAAATCTGA CGTTCAGGGA ACGCCAGCGA CTTTTTTCAC AGGGTCAAGA TGTATCCAAT
    AAAGTAAAAG CCTCTAGGAA ACTAATGGAA CTGGAAAATG AGTTGAACAC GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33798
    Clone ID Related Accession (Same CDS sequence) XM_015284348.2 , XM_015284348.1
    Accession Version XM_015284348.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284348.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGAAACAGA AGAATATTGC TGGGGCACTT GCCTTTTGGA TGGCAAATGC GTCTGAATTG
    CTGAATTTTA TAAAGCAAGA CCGTGATCTG AGCCGAATCA CTGTGGATGC TCAAGATGTT
    TTGGCGCACT TGGTTCAAAT GGCATTCAAG TACCTAGTTC ATTGTCTGCA GTCAGAGCTT
    AATAACTACA TGCCAGCATT TCTTGATGAT CCAGAGGAAA ATAATCCACA GAGGCCTAAA
    ATAGATGATG TGCTGCACAC ATTGACTGGA GCTATGTCCT TGTTGCGACG ATGTAGAGTG
    AATGCTGCAC TGACTATACA GCTCTTCTCC CAGTTGTTTC ATTTTATCAA CATGTGGCTT
    TTTAACAGAC TAGTTACGGC TCCAGATTCA GGGTTATGTT CCCACTACTG GGGAGCTATT
    GTTCGTCAGC AGTTGGGTCA TATTGAGGCC TGGGCAGAAA AACAGGGTTT AGAATTGGCT
    GCTGATTGCC ACTTAAGCAG AATAGTGCAG GCAACCACAT TGCTCACCAT GGACAAGTAC
    TCACATGCTG ATGTTCCAAA TATTAACAGT ACTTGCTTTA AGTTGAATTC TCTACAGCTA
    CAAGCTTTGT TGCAGAATTA TCACTGTGCT CCAGATGAAC CGCTTATACC AACAGAACTG
    ATAGAGAATG TAGTAACTGT TGCAGAAAAT ACGGCTGATG AACTTGCTCG CAGTGATGGC
    AGAGAAGTCC AATTGGAAGA GGATCCTGAC TTACAACTCC CTTTTCTTTT ACCAGAAGAT
    GGCTATTCCT GTGATGTTGT CAGAAATGTT CCAAGTGGTT TACAAGAATT TTTGGATCCC
    CTCTGTCAGA GAGGTTTTTG TAGGTTAATA CCTCATACAC GGTCACCAGG CACATGGACA
    ATATACTTTG AAGGCGCAGA CTATGAAAGC CACCTGTCAC ATGAGAATGC TGAGCTAGCT
    CAGCCTTTGA GGAAAGAACC TGAGATTATA ACTGTAACAC TAAAAAAACA AAATGGAATG
    GGCCTCAGCA TTGTTGCTGC CAAGGGTGCT GGTCAAGACA AACTTGGAAT ATATGTCAAG
    TCTGTTGTGA AAGGAGGTGC TGCAGATGTG GATGGTCGTC TGGCAGCAGG GGATCAGCTG
    CTCAGTGTGG ATGGTCGAAG CTTGGTGGGG CTGTCCCAGG AAAGGGCAGC AGAACTTATG
    ACGAGGACAG GTTCTGTTGT AACACTGGAA GTAGCAAAAC AAGGAGCAAT ATACCACGGT
    CTGGCAACCC TGCTAAATCA GCCATCTCCA ATGATGCAAA GAGTTTCTGA CCGCCGTGGC
    TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTTGAGCTGT ATAACAACTC CACGCAAAAT
    GGGTCACCTG AGAGCCCCCA GCTGCCATGG ACGGAGTACA GTGAACCAAA GAAGCTGCCA
    GGAGATGACA GATTAATGAA GAACAGAGCC GATCATCGCT CCAGCCCCAA CGTAGCAAAT
    CAGCCTCCAA GCCCTGGGGG GAAGAATGCT TATGCATCTG GAACTACCAC CAAAATAACC
    TCAGTCTCGA CTGGAAATCT TTGTACAGAG GAGCAAACTC TGCCTCCACG ACCTGAAGCT
    TATCCTATCC CAACACAGAC CTATACCAGA GAATATTTCA CATTCCCAGC TTCAAAATCC
    CAGGATCGAA TGGGTCCAGC TCAGAGTCAG TGGCCAAATT ATGAAGAGAA GCCACAGATC
    CAGGCAGAAA GTAATCACTC AATCAATACT ACAATGCAGC GCGTGGCTCG ATCCCAGGAA
    GAACTCCGAG ATAAAGTTTT CCAGCCAGAG AGGAATCGTT TGGAAGTTGT TATCCGGAAG
    GACATAGAAT ACATTGATCG GAAGTCAGAC AGCGATCAGT GGATGAATTC ATCTGTGGAT
    TCTAGCACAT CTAGCCAAGA GCACCTGAAC CATACATCCA AACCTGTTTC ACCTGGTTCT
    TGCTTAACTA AAAGTGGACC TGGCCGTTGG AAAACTCCAG CTACGAGCCA GCCAACTCCA
    GTGGCCGTTT CCCAGCCCAT CAGAACAGAC CTTCCTCCTC CGCCGCCACC TCCTCCGGTG
    CATTATGCAG CTGAATTTGA TGAACCACAA ATGGATTTGC CTCTGCCTCC CCCTCCTCCA
    CCGAACCAAG TAGGAGCACA AGCATCTTCT CAGGTGGCTG CTGCTGAGCG TAAAAAACGA
    GAAGAGCATC AGAGGTGGTA TGAAAAGGAA AAGGCACGTT TGGAAGAAGA ACGAGAAAGG
    AAACGCCGTG AGCAAGAGAG AAAACTGGGC CAAATGCGTT CGCAGCCACT AATTCCTCCC
    CAGCCTGTGG TTCCTCCTGT TTCCCTTCAG CAAGTGAAAT CGGAAAAAAC TTCAACTTTC
    CAGAGGTCTC AAGAAACAGT CATTCGAGAG TTGCAGCCGC AACAGCAGCC TCGGACTATT
    GAGCGGAGAG ATCTGCAGTA CATTACTATC AGCAAGGAAG AATTGTCCTC TAGTGACAGC
    CTCTCTCCAG ATCCCTGGAA ACGAGATGCC AAGGAGAAAC TGGAGAAGCA GCAGCAGATG
    CACATTGTGG ACATGCTGAG CAAAGAGATT CAGGATCTTC AGAACAAGCC TGATCGTACA
    GCTGAAGAGA ACGACCGCCT GCGCAAACTC ATGCTAGAGT GGCAGTTCCA AAAGCGACTT
    CAGGAATCAA AACAAAAGGA TGAAGATGAT GATGAAGAAG AGGACGATGA TGTGGATACT
    ATGCTTATCA TGCAGCGGCT GGAGGCAGAA AGAAGAGCAA GGCAGACTGC TGTGCCAGCA
    ATCTCTGTTC TAGACTTGTT ACAGGATGAA GAACGCAGAC GACAGCAACA GTTAGAAGAA
    ATGCGCAAGC GAGAAGCAGA AGACAGGGCC AGGCAGGAAG AGGAGCGCCG ACAGCAAGAG
    GAGGAGCGAA CCAGGAGAGA GGCAGAAGAA AAGAGGCGAC AGGAAGAGGA GTATTACAAT
    CGCTTAGAGG CTGAAAGGCG CAGGCAGCAC GATGAAGCAG AACGAAGATT GCTGGAACCA
    GACGAGCCTG GTCTGTACAG ACCTCCACTT CCACGGGAAT ATGAACTCCC ATCCCCAACC
    CCTTCATCTA ACGCTCCTCC TCCCCCACCT CAGCGAAACA CCTCTTACCT CAAAACACAG
    GTCCTCTCCC CTGACACGAT TTATACTGCC AAGTTTGTTG CATACAATGA TGATGAGGAG
    GAGGAGGATT CCAATCTAGC AGGTCAGGAT AAGTACTCCA GCACAAGAAA ATCTTATGGT
    GACTTTCCTC CTGCCACCCT TAAACCACAG CAGCCCTCCC GCCAGCCTCG CCCAGTTTCA
    GATGGCCTCT TTCTTTCCAA CTCATTCCAG TCATCTCCAG TCAATGCCAA CAGTACTGCT
    GCCAAAAAAA GCCAGCCCCC GCCCCCACCA AACAAACCGA GTTTCATCCA TGCCAGCAGC
    TCTAAAGGAA TAAATTCATA CACTGGATCT TCGGGAGCAA CGGTTGGGGC CCATGAAGTC
    TATCGGGATC CAAGAGACAA ACGATCTAAA AGTCAAGATA CAGATTTACC TGGAGCACCA
    GAAAATCTGA CGTTCAGGGA ACGCCAGCGA CTTTTTTCAC AGGGTCAAGA TGTATCCAAT
    AAAGTAAAAG CCTCTAGGAA ACTAATGGAA CTGGAAAATG AGTTGAACAC GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139834.1
    CDS2885..8641
    Translation

    Target ORF information:

    RefSeq Version XM_015284348.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284348.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGAAACAGA AGAATATTGC TGGGGCACTT GCCTTTTGGA TGGCAAATGC GTCTGAATTG
    CTGAATTTTA TAAAGCAAGA CCGTGATCTG AGCCGAATCA CTGTGGATGC TCAAGATGTT
    TTGGCGCACT TGGTTCAAAT GGCATTCAAG TACCTAGTTC ATTGTCTGCA GTCAGAGCTT
    AATAACTACA TGCCAGCATT TCTTGATGAT CCAGAGGAAA ATAATCCACA GAGGCCTAAA
    ATAGATGATG TGCTGCACAC ATTGACTGGA GCTATGTCCT TGTTGCGACG ATGTAGAGTG
    AATGCTGCAC TGACTATACA GCTCTTCTCC CAGTTGTTTC ATTTTATCAA CATGTGGCTT
    TTTAACAGAC TAGTTACGGC TCCAGATTCA GGGTTATGTT CCCACTACTG GGGAGCTATT
    GTTCGTCAGC AGTTGGGTCA TATTGAGGCC TGGGCAGAAA AACAGGGTTT AGAATTGGCT
    GCTGATTGCC ACTTAAGCAG AATAGTGCAG GCAACCACAT TGCTCACCAT GGACAAGTAC
    TCACATGCTG ATGTTCCAAA TATTAACAGT ACTTGCTTTA AGTTGAATTC TCTACAGCTA
    CAAGCTTTGT TGCAGAATTA TCACTGTGCT CCAGATGAAC CGCTTATACC AACAGAACTG
    ATAGAGAATG TAGTAACTGT TGCAGAAAAT ACGGCTGATG AACTTGCTCG CAGTGATGGC
    AGAGAAGTCC AATTGGAAGA GGATCCTGAC TTACAACTCC CTTTTCTTTT ACCAGAAGAT
    GGCTATTCCT GTGATGTTGT CAGAAATGTT CCAAGTGGTT TACAAGAATT TTTGGATCCC
    CTCTGTCAGA GAGGTTTTTG TAGGTTAATA CCTCATACAC GGTCACCAGG CACATGGACA
    ATATACTTTG AAGGCGCAGA CTATGAAAGC CACCTGTCAC ATGAGAATGC TGAGCTAGCT
    CAGCCTTTGA GGAAAGAACC TGAGATTATA ACTGTAACAC TAAAAAAACA AAATGGAATG
    GGCCTCAGCA TTGTTGCTGC CAAGGGTGCT GGTCAAGACA AACTTGGAAT ATATGTCAAG
    TCTGTTGTGA AAGGAGGTGC TGCAGATGTG GATGGTCGTC TGGCAGCAGG GGATCAGCTG
    CTCAGTGTGG ATGGTCGAAG CTTGGTGGGG CTGTCCCAGG AAAGGGCAGC AGAACTTATG
    ACGAGGACAG GTTCTGTTGT AACACTGGAA GTAGCAAAAC AAGGAGCAAT ATACCACGGT
    CTGGCAACCC TGCTAAATCA GCCATCTCCA ATGATGCAAA GAGTTTCTGA CCGCCGTGGC
    TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTTGAGCTGT ATAACAACTC CACGCAAAAT
    GGGTCACCTG AGAGCCCCCA GCTGCCATGG ACGGAGTACA GTGAACCAAA GAAGCTGCCA
    GGAGATGACA GATTAATGAA GAACAGAGCC GATCATCGCT CCAGCCCCAA CGTAGCAAAT
    CAGCCTCCAA GCCCTGGGGG GAAGAATGCT TATGCATCTG GAACTACCAC CAAAATAACC
    TCAGTCTCGA CTGGAAATCT TTGTACAGAG GAGCAAACTC TGCCTCCACG ACCTGAAGCT
    TATCCTATCC CAACACAGAC CTATACCAGA GAATATTTCA CATTCCCAGC TTCAAAATCC
    CAGGATCGAA TGGGTCCAGC TCAGAGTCAG TGGCCAAATT ATGAAGAGAA GCCACAGATC
    CAGGCAGAAA GTAATCACTC AATCAATACT ACAATGCAGC GCGTGGCTCG ATCCCAGGAA
    GAACTCCGAG ATAAAGTTTT CCAGCCAGAG AGGAATCGTT TGGAAGTTGT TATCCGGAAG
    GACATAGAAT ACATTGATCG GAAGTCAGAC AGCGATCAGT GGATGAATTC ATCTGTGGAT
    TCTAGCACAT CTAGCCAAGA GCACCTGAAC CATACATCCA AACCTGTTTC ACCTGGTTCT
    TGCTTAACTA AAAGTGGACC TGGCCGTTGG AAAACTCCAG CTACGAGCCA GCCAACTCCA
    GTGGCCGTTT CCCAGCCCAT CAGAACAGAC CTTCCTCCTC CGCCGCCACC TCCTCCGGTG
    CATTATGCAG CTGAATTTGA TGAACCACAA ATGGATTTGC CTCTGCCTCC CCCTCCTCCA
    CCGAACCAAG TAGGAGCACA AGCATCTTCT CAGGTGGCTG CTGCTGAGCG TAAAAAACGA
    GAAGAGCATC AGAGGTGGTA TGAAAAGGAA AAGGCACGTT TGGAAGAAGA ACGAGAAAGG
    AAACGCCGTG AGCAAGAGAG AAAACTGGGC CAAATGCGTT CGCAGCCACT AATTCCTCCC
    CAGCCTGTGG TTCCTCCTGT TTCCCTTCAG CAAGTGAAAT CGGAAAAAAC TTCAACTTTC
    CAGAGGTCTC AAGAAACAGT CATTCGAGAG TTGCAGCCGC AACAGCAGCC TCGGACTATT
    GAGCGGAGAG ATCTGCAGTA CATTACTATC AGCAAGGAAG AATTGTCCTC TAGTGACAGC
    CTCTCTCCAG ATCCCTGGAA ACGAGATGCC AAGGAGAAAC TGGAGAAGCA GCAGCAGATG
    CACATTGTGG ACATGCTGAG CAAAGAGATT CAGGATCTTC AGAACAAGCC TGATCGTACA
    GCTGAAGAGA ACGACCGCCT GCGCAAACTC ATGCTAGAGT GGCAGTTCCA AAAGCGACTT
    CAGGAATCAA AACAAAAGGA TGAAGATGAT GATGAAGAAG AGGACGATGA TGTGGATACT
    ATGCTTATCA TGCAGCGGCT GGAGGCAGAA AGAAGAGCAA GGCAGACTGC TGTGCCAGCA
    ATCTCTGTTC TAGACTTGTT ACAGGATGAA GAACGCAGAC GACAGCAACA GTTAGAAGAA
    ATGCGCAAGC GAGAAGCAGA AGACAGGGCC AGGCAGGAAG AGGAGCGCCG ACAGCAAGAG
    GAGGAGCGAA CCAGGAGAGA GGCAGAAGAA AAGAGGCGAC AGGAAGAGGA GTATTACAAT
    CGCTTAGAGG CTGAAAGGCG CAGGCAGCAC GATGAAGCAG AACGAAGATT GCTGGAACCA
    GACGAGCCTG GTCTGTACAG ACCTCCACTT CCACGGGAAT ATGAACTCCC ATCCCCAACC
    CCTTCATCTA ACGCTCCTCC TCCCCCACCT CAGCGAAACA CCTCTTACCT CAAAACACAG
    GTCCTCTCCC CTGACACGAT TTATACTGCC AAGTTTGTTG CATACAATGA TGATGAGGAG
    GAGGAGGATT CCAATCTAGC AGGTCAGGAT AAGTACTCCA GCACAAGAAA ATCTTATGGT
    GACTTTCCTC CTGCCACCCT TAAACCACAG CAGCCCTCCC GCCAGCCTCG CCCAGTTTCA
    GATGGCCTCT TTCTTTCCAA CTCATTCCAG TCATCTCCAG TCAATGCCAA CAGTACTGCT
    GCCAAAAAAA GCCAGCCCCC GCCCCCACCA AACAAACCGA GTTTCATCCA TGCCAGCAGC
    TCTAAAGGAA TAAATTCATA CACTGGATCT TCGGGAGCAA CGGTTGGGGC CCATGAAGTC
    TATCGGGATC CAAGAGACAA ACGATCTAAA AGTCAAGATA CAGATTTACC TGGAGCACCA
    GAAAATCTGA CGTTCAGGGA ACGCCAGCGA CTTTTTTCAC AGGGTCAAGA TGTATCCAAT
    AAAGTAAAAG CCTCTAGGAA ACTAATGGAA CTGGAAAATG AGTTGAACAC GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33798
    Clone ID Related Accession (Same CDS sequence) XM_015284348.2 , XM_015284348.1
    Accession Version XM_015284348.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGAAACAGA AGAATATTGC TGGGGCACTT GCCTTTTGGA TGGCAAATGC GTCTGAATTG
    CTGAATTTTA TAAAGCAAGA CCGTGATCTG AGCCGAATCA CTGTGGATGC TCAAGATGTT
    TTGGCGCACT TGGTTCAAAT GGCATTCAAG TACCTAGTTC ATTGTCTGCA GTCAGAGCTT
    AATAACTACA TGCCAGCATT TCTTGATGAT CCAGAGGAAA ATAATCCACA GAGGCCTAAA
    ATAGATGATG TGCTGCACAC ATTGACTGGA GCTATGTCCT TGTTGCGACG ATGTAGAGTG
    AATGCTGCAC TGACTATACA GCTCTTCTCC CAGTTGTTTC ATTTTATCAA CATGTGGCTT
    TTTAACAGAC TAGTTACGGC TCCAGATTCA GGGTTATGTT CCCACTACTG GGGAGCTATT
    GTTCGTCAGC AGTTGGGTCA TATTGAGGCC TGGGCAGAAA AACAGGGTTT AGAATTGGCT
    GCTGATTGCC ACTTAAGCAG AATAGTGCAG GCAACCACAT TGCTCACCAT GGACAAGTAC
    TCACATGCTG ATGTTCCAAA TATTAACAGT ACTTGCTTTA AGTTGAATTC TCTACAGCTA
    CAAGCTTTGT TGCAGAATTA TCACTGTGCT CCAGATGAAC CGCTTATACC AACAGAACTG
    ATAGAGAATG TAGTAACTGT TGCAGAAAAT ACGGCTGATG AACTTGCTCG CAGTGATGGC
    AGAGAAGTCC AATTGGAAGA GGATCCTGAC TTACAACTCC CTTTTCTTTT ACCAGAAGAT
    GGCTATTCCT GTGATGTTGT CAGAAATGTT CCAAGTGGTT TACAAGAATT TTTGGATCCC
    CTCTGTCAGA GAGGTTTTTG TAGGTTAATA CCTCATACAC GGTCACCAGG CACATGGACA
    ATATACTTTG AAGGCGCAGA CTATGAAAGC CACCTGTCAC ATGAGAATGC TGAGCTAGCT
    CAGCCTTTGA GGAAAGAACC TGAGATTATA ACTGTAACAC TAAAAAAACA AAATGGAATG
    GGCCTCAGCA TTGTTGCTGC CAAGGGTGCT GGTCAAGACA AACTTGGAAT ATATGTCAAG
    TCTGTTGTGA AAGGAGGTGC TGCAGATGTG GATGGTCGTC TGGCAGCAGG GGATCAGCTG
    CTCAGTGTGG ATGGTCGAAG CTTGGTGGGG CTGTCCCAGG AAAGGGCAGC AGAACTTATG
    ACGAGGACAG GTTCTGTTGT AACACTGGAA GTAGCAAAAC AAGGAGCAAT ATACCACGGT
    CTGGCAACCC TGCTAAATCA GCCATCTCCA ATGATGCAAA GAGTTTCTGA CCGCCGTGGC
    TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTTGAGCTGT ATAACAACTC CACGCAAAAT
    GGGTCACCTG AGAGCCCCCA GCTGCCATGG ACGGAGTACA GTGAACCAAA GAAGCTGCCA
    GGAGATGACA GATTAATGAA GAACAGAGCC GATCATCGCT CCAGCCCCAA CGTAGCAAAT
    CAGCCTCCAA GCCCTGGGGG GAAGAATGCT TATGCATCTG GAACTACCAC CAAAATAACC
    TCAGTCTCGA CTGGAAATCT TTGTACAGAG GAGCAAACTC TGCCTCCACG ACCTGAAGCT
    TATCCTATCC CAACACAGAC CTATACCAGA GAATATTTCA CATTCCCAGC TTCAAAATCC
    CAGGATCGAA TGGGTCCAGC TCAGAGTCAG TGGCCAAATT ATGAAGAGAA GCCACAGATC
    CAGGCAGAAA GTAATCACTC AATCAATACT ACAATGCAGC GCGTGGCTCG ATCCCAGGAA
    GAACTCCGAG ATAAAGTTTT CCAGCCAGAG AGGAATCGTT TGGAAGTTGT TATCCGGAAG
    GACATAGAAT ACATTGATCG GAAGTCAGAC AGCGATCAGT GGATGAATTC ATCTGTGGAT
    TCTAGCACAT CTAGCCAAGA GCACCTGAAC CATACATCCA AACCTGTTTC ACCTGGTTCT
    TGCTTAACTA AAAGTGGACC TGGCCGTTGG AAAACTCCAG CTACGAGCCA GCCAACTCCA
    GTGGCCGTTT CCCAGCCCAT CAGAACAGAC CTTCCTCCTC CGCCGCCACC TCCTCCGGTG
    CATTATGCAG CTGAATTTGA TGAACCACAA ATGGATTTGC CTCTGCCTCC CCCTCCTCCA
    CCGAACCAAG TAGGAGCACA AGCATCTTCT CAGGTGGCTG CTGCTGAGCG TAAAAAACGA
    GAAGAGCATC AGAGGTGGTA TGAAAAGGAA AAGGCACGTT TGGAAGAAGA ACGAGAAAGG
    AAACGCCGTG AGCAAGAGAG AAAACTGGGC CAAATGCGTT CGCAGCCACT AATTCCTCCC
    CAGCCTGTGG TTCCTCCTGT TTCCCTTCAG CAAGTGAAAT CGGAAAAAAC TTCAACTTTC
    CAGAGGTCTC AAGAAACAGT CATTCGAGAG TTGCAGCCGC AACAGCAGCC TCGGACTATT
    GAGCGGAGAG ATCTGCAGTA CATTACTATC AGCAAGGAAG AATTGTCCTC TAGTGACAGC
    CTCTCTCCAG ATCCCTGGAA ACGAGATGCC AAGGAGAAAC TGGAGAAGCA GCAGCAGATG
    CACATTGTGG ACATGCTGAG CAAAGAGATT CAGGATCTTC AGAACAAGCC TGATCGTACA
    GCTGAAGAGA ACGACCGCCT GCGCAAACTC ATGCTAGAGT GGCAGTTCCA AAAGCGACTT
    CAGGAATCAA AACAAAAGGA TGAAGATGAT GATGAAGAAG AGGACGATGA TGTGGATACT
    ATGCTTATCA TGCAGCGGCT GGAGGCAGAA AGAAGAGCAA GGCAGACTGC TGTGCCAGCA
    ATCTCTGTTC TAGACTTGTT ACAGGATGAA GAACGCAGAC GACAGCAACA GTTAGAAGAA
    ATGCGCAAGC GAGAAGCAGA AGACAGGGCC AGGCAGGAAG AGGAGCGCCG ACAGCAAGAG
    GAGGAGCGAA CCAGGAGAGA GGCAGAAGAA AAGAGGCGAC AGGAAGAGGA GTATTACAAT
    CGCTTAGAGG CTGAAAGGCG CAGGCAGCAC GATGAAGCAG AACGAAGATT GCTGGAACCA
    GACGAGCCTG GTCTGTACAG ACCTCCACTT CCACGGGAAT ATGAACTCCC ATCCCCAACC
    CCTTCATCTA ACGCTCCTCC TCCCCCACCT CAGCGAAACA CCTCTTACCT CAAAACACAG
    GTCCTCTCCC CTGACACGAT TTATACTGCC AAGTTTGTTG CATACAATGA TGATGAGGAG
    GAGGAGGATT CCAATCTAGC AGGTCAGGAT AAGTACTCCA GCACAAGAAA ATCTTATGGT
    GACTTTCCTC CTGCCACCCT TAAACCACAG CAGCCCTCCC GCCAGCCTCG CCCAGTTTCA
    GATGGCCTCT TTCTTTCCAA CTCATTCCAG TCATCTCCAG TCAATGCCAA CAGTACTGCT
    GCCAAAAAAA GCCAGCCCCC GCCCCCACCA AACAAACCGA GTTTCATCCA TGCCAGCAGC
    TCTAAAGGAA TAAATTCATA CACTGGATCT TCGGGAGCAA CGGTTGGGGC CCATGAAGTC
    TATCGGGATC CAAGAGACAA ACGATCTAAA AGTCAAGATA CAGATTTACC TGGAGCACCA
    GAAAATCTGA CGTTCAGGGA ACGCCAGCGA CTTTTTTCAC AGGGTCAAGA TGTATCCAAT
    AAAGTAAAAG CCTCTAGGAA ACTAATGGAA CTGGAAAATG AGTTGAACAC GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139834.1
    CDS33..5789
    Translation

    Target ORF information:

    RefSeq Version XM_015284348.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284348.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGAAACAGA AGAATATTGC TGGGGCACTT GCCTTTTGGA TGGCAAATGC GTCTGAATTG
    CTGAATTTTA TAAAGCAAGA CCGTGATCTG AGCCGAATCA CTGTGGATGC TCAAGATGTT
    TTGGCGCACT TGGTTCAAAT GGCATTCAAG TACCTAGTTC ATTGTCTGCA GTCAGAGCTT
    AATAACTACA TGCCAGCATT TCTTGATGAT CCAGAGGAAA ATAATCCACA GAGGCCTAAA
    ATAGATGATG TGCTGCACAC ATTGACTGGA GCTATGTCCT TGTTGCGACG ATGTAGAGTG
    AATGCTGCAC TGACTATACA GCTCTTCTCC CAGTTGTTTC ATTTTATCAA CATGTGGCTT
    TTTAACAGAC TAGTTACGGC TCCAGATTCA GGGTTATGTT CCCACTACTG GGGAGCTATT
    GTTCGTCAGC AGTTGGGTCA TATTGAGGCC TGGGCAGAAA AACAGGGTTT AGAATTGGCT
    GCTGATTGCC ACTTAAGCAG AATAGTGCAG GCAACCACAT TGCTCACCAT GGACAAGTAC
    TCACATGCTG ATGTTCCAAA TATTAACAGT ACTTGCTTTA AGTTGAATTC TCTACAGCTA
    CAAGCTTTGT TGCAGAATTA TCACTGTGCT CCAGATGAAC CGCTTATACC AACAGAACTG
    ATAGAGAATG TAGTAACTGT TGCAGAAAAT ACGGCTGATG AACTTGCTCG CAGTGATGGC
    AGAGAAGTCC AATTGGAAGA GGATCCTGAC TTACAACTCC CTTTTCTTTT ACCAGAAGAT
    GGCTATTCCT GTGATGTTGT CAGAAATGTT CCAAGTGGTT TACAAGAATT TTTGGATCCC
    CTCTGTCAGA GAGGTTTTTG TAGGTTAATA CCTCATACAC GGTCACCAGG CACATGGACA
    ATATACTTTG AAGGCGCAGA CTATGAAAGC CACCTGTCAC ATGAGAATGC TGAGCTAGCT
    CAGCCTTTGA GGAAAGAACC TGAGATTATA ACTGTAACAC TAAAAAAACA AAATGGAATG
    GGCCTCAGCA TTGTTGCTGC CAAGGGTGCT GGTCAAGACA AACTTGGAAT ATATGTCAAG
    TCTGTTGTGA AAGGAGGTGC TGCAGATGTG GATGGTCGTC TGGCAGCAGG GGATCAGCTG
    CTCAGTGTGG ATGGTCGAAG CTTGGTGGGG CTGTCCCAGG AAAGGGCAGC AGAACTTATG
    ACGAGGACAG GTTCTGTTGT AACACTGGAA GTAGCAAAAC AAGGAGCAAT ATACCACGGT
    CTGGCAACCC TGCTAAATCA GCCATCTCCA ATGATGCAAA GAGTTTCTGA CCGCCGTGGC
    TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTTGAGCTGT ATAACAACTC CACGCAAAAT
    GGGTCACCTG AGAGCCCCCA GCTGCCATGG ACGGAGTACA GTGAACCAAA GAAGCTGCCA
    GGAGATGACA GATTAATGAA GAACAGAGCC GATCATCGCT CCAGCCCCAA CGTAGCAAAT
    CAGCCTCCAA GCCCTGGGGG GAAGAATGCT TATGCATCTG GAACTACCAC CAAAATAACC
    TCAGTCTCGA CTGGAAATCT TTGTACAGAG GAGCAAACTC TGCCTCCACG ACCTGAAGCT
    TATCCTATCC CAACACAGAC CTATACCAGA GAATATTTCA CATTCCCAGC TTCAAAATCC
    CAGGATCGAA TGGGTCCAGC TCAGAGTCAG TGGCCAAATT ATGAAGAGAA GCCACAGATC
    CAGGCAGAAA GTAATCACTC AATCAATACT ACAATGCAGC GCGTGGCTCG ATCCCAGGAA
    GAACTCCGAG ATAAAGTTTT CCAGCCAGAG AGGAATCGTT TGGAAGTTGT TATCCGGAAG
    GACATAGAAT ACATTGATCG GAAGTCAGAC AGCGATCAGT GGATGAATTC ATCTGTGGAT
    TCTAGCACAT CTAGCCAAGA GCACCTGAAC CATACATCCA AACCTGTTTC ACCTGGTTCT
    TGCTTAACTA AAAGTGGACC TGGCCGTTGG AAAACTCCAG CTACGAGCCA GCCAACTCCA
    GTGGCCGTTT CCCAGCCCAT CAGAACAGAC CTTCCTCCTC CGCCGCCACC TCCTCCGGTG
    CATTATGCAG CTGAATTTGA TGAACCACAA ATGGATTTGC CTCTGCCTCC CCCTCCTCCA
    CCGAACCAAG TAGGAGCACA AGCATCTTCT CAGGTGGCTG CTGCTGAGCG TAAAAAACGA
    GAAGAGCATC AGAGGTGGTA TGAAAAGGAA AAGGCACGTT TGGAAGAAGA ACGAGAAAGG
    AAACGCCGTG AGCAAGAGAG AAAACTGGGC CAAATGCGTT CGCAGCCACT AATTCCTCCC
    CAGCCTGTGG TTCCTCCTGT TTCCCTTCAG CAAGTGAAAT CGGAAAAAAC TTCAACTTTC
    CAGAGGTCTC AAGAAACAGT CATTCGAGAG TTGCAGCCGC AACAGCAGCC TCGGACTATT
    GAGCGGAGAG ATCTGCAGTA CATTACTATC AGCAAGGAAG AATTGTCCTC TAGTGACAGC
    CTCTCTCCAG ATCCCTGGAA ACGAGATGCC AAGGAGAAAC TGGAGAAGCA GCAGCAGATG
    CACATTGTGG ACATGCTGAG CAAAGAGATT CAGGATCTTC AGAACAAGCC TGATCGTACA
    GCTGAAGAGA ACGACCGCCT GCGCAAACTC ATGCTAGAGT GGCAGTTCCA AAAGCGACTT
    CAGGAATCAA AACAAAAGGA TGAAGATGAT GATGAAGAAG AGGACGATGA TGTGGATACT
    ATGCTTATCA TGCAGCGGCT GGAGGCAGAA AGAAGAGCAA GGCAGACTGC TGTGCCAGCA
    ATCTCTGTTC TAGACTTGTT ACAGGATGAA GAACGCAGAC GACAGCAACA GTTAGAAGAA
    ATGCGCAAGC GAGAAGCAGA AGACAGGGCC AGGCAGGAAG AGGAGCGCCG ACAGCAAGAG
    GAGGAGCGAA CCAGGAGAGA GGCAGAAGAA AAGAGGCGAC AGGAAGAGGA GTATTACAAT
    CGCTTAGAGG CTGAAAGGCG CAGGCAGCAC GATGAAGCAG AACGAAGATT GCTGGAACCA
    GACGAGCCTG GTCTGTACAG ACCTCCACTT CCACGGGAAT ATGAACTCCC ATCCCCAACC
    CCTTCATCTA ACGCTCCTCC TCCCCCACCT CAGCGAAACA CCTCTTACCT CAAAACACAG
    GTCCTCTCCC CTGACACGAT TTATACTGCC AAGTTTGTTG CATACAATGA TGATGAGGAG
    GAGGAGGATT CCAATCTAGC AGGTCAGGAT AAGTACTCCA GCACAAGAAA ATCTTATGGT
    GACTTTCCTC CTGCCACCCT TAAACCACAG CAGCCCTCCC GCCAGCCTCG CCCAGTTTCA
    GATGGCCTCT TTCTTTCCAA CTCATTCCAG TCATCTCCAG TCAATGCCAA CAGTACTGCT
    GCCAAAAAAA GCCAGCCCCC GCCCCCACCA AACAAACCGA GTTTCATCCA TGCCAGCAGC
    TCTAAAGGAA TAAATTCATA CACTGGATCT TCGGGAGCAA CGGTTGGGGC CCATGAAGTC
    TATCGGGATC CAAGAGACAA ACGATCTAAA AGTCAAGATA CAGATTTACC TGGAGCACCA
    GAAAATCTGA CGTTCAGGGA ACGCCAGCGA CTTTTTTCAC AGGGTCAAGA TGTATCCAAT
    AAAGTAAAAG CCTCTAGGAA ACTAATGGAA CTGGAAAATG AGTTGAACAC GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33799
    Clone ID Related Accession (Same CDS sequence) XM_015284349.1 , XM_015284349.2
    Accession Version XM_015284349.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGTTACAGG ATGAAGAACG CAGACGACAG CAACAGTTAG AAGAAATGCG CAAGCGAGAA
    GCAGAAGACA GGGCCAGGCA GGAAGAGGAG CGCCGACAGC AAGAGGAGGA GCGAACCAGG
    AGAGAGGCAG AAGAAAAGAG GCGACAGGAA GAGGAGTATT ACAATCGCTT AGAGGCTGAA
    AGGCGCAGGC AGCACGATGA AGCAGAACGA AGATTGCTGG AACCAGACGA GCCTGGTCTG
    TACAGACCTC CACTTCCACG GGAATATGAA CTCCCATCCC CAACCCCTTC ATCTAACGCT
    CCTCCTCCCC CACCTCAGCG AAACACCTCT TACCTCAAAA CACAGGTCCT CTCCCCTGAC
    ACGATTTATA CTGCCAAGTT TGTTGCATAC AATGATGATG AGGAGGAGGA GGATTCCAAT
    CTAGCAGGTC AGGATAAGTA CTCCAGCACA AGAAAATCTT ATGGTGACTT TCCTCCTGCC
    ACCCTTAAAC CACAGCAGCC CTCCCGCCAG CCTCGCCCAG TTTCAGATGG CCTCTTTCTT
    TCCAACTCAT TCCAGTCATC TCCAGTCAAT GCCAACAGTA CTGCTGCCAA AAAAAGCCAG
    CCCCCGCCCC CACCAAACAA ACCGAGTTTC ATCCATGCCA GCAGCTCTAA AGGAATAAAT
    TCATACACTG GATCTTCGGG AGCAACGGTT GGGGCCCATG AAGTCTATCG GGATCCAAGA
    GACAAACGAT CTAAAAGTCA AGATACAGAT TTACCTGGAG CACCAGAAAA TCTGACGTTC
    AGGGAACGCC AGCGACTTTT TTCACAGGGT CAAGATGTAT CCAATAAAGT AAAAGCCTCT
    AGGAAACTAA TGGAACTGGA AAATGAGTTG AACACGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139835.1
    CDS33..5774
    Translation

    Target ORF information:

    RefSeq Version XM_015284349.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284349.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGTTACAGG ATGAAGAACG CAGACGACAG CAACAGTTAG AAGAAATGCG CAAGCGAGAA
    GCAGAAGACA GGGCCAGGCA GGAAGAGGAG CGCCGACAGC AAGAGGAGGA GCGAACCAGG
    AGAGAGGCAG AAGAAAAGAG GCGACAGGAA GAGGAGTATT ACAATCGCTT AGAGGCTGAA
    AGGCGCAGGC AGCACGATGA AGCAGAACGA AGATTGCTGG AACCAGACGA GCCTGGTCTG
    TACAGACCTC CACTTCCACG GGAATATGAA CTCCCATCCC CAACCCCTTC ATCTAACGCT
    CCTCCTCCCC CACCTCAGCG AAACACCTCT TACCTCAAAA CACAGGTCCT CTCCCCTGAC
    ACGATTTATA CTGCCAAGTT TGTTGCATAC AATGATGATG AGGAGGAGGA GGATTCCAAT
    CTAGCAGGTC AGGATAAGTA CTCCAGCACA AGAAAATCTT ATGGTGACTT TCCTCCTGCC
    ACCCTTAAAC CACAGCAGCC CTCCCGCCAG CCTCGCCCAG TTTCAGATGG CCTCTTTCTT
    TCCAACTCAT TCCAGTCATC TCCAGTCAAT GCCAACAGTA CTGCTGCCAA AAAAAGCCAG
    CCCCCGCCCC CACCAAACAA ACCGAGTTTC ATCCATGCCA GCAGCTCTAA AGGAATAAAT
    TCATACACTG GATCTTCGGG AGCAACGGTT GGGGCCCATG AAGTCTATCG GGATCCAAGA
    GACAAACGAT CTAAAAGTCA AGATACAGAT TTACCTGGAG CACCAGAAAA TCTGACGTTC
    AGGGAACGCC AGCGACTTTT TTCACAGGGT CAAGATGTAT CCAATAAAGT AAAAGCCTCT
    AGGAAACTAA TGGAACTGGA AAATGAGTTG AACACGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33799
    Clone ID Related Accession (Same CDS sequence) XM_015284349.1 , XM_015284349.2
    Accession Version XM_015284349.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284349.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGTTACAGG ATGAAGAACG CAGACGACAG CAACAGTTAG AAGAAATGCG CAAGCGAGAA
    GCAGAAGACA GGGCCAGGCA GGAAGAGGAG CGCCGACAGC AAGAGGAGGA GCGAACCAGG
    AGAGAGGCAG AAGAAAAGAG GCGACAGGAA GAGGAGTATT ACAATCGCTT AGAGGCTGAA
    AGGCGCAGGC AGCACGATGA AGCAGAACGA AGATTGCTGG AACCAGACGA GCCTGGTCTG
    TACAGACCTC CACTTCCACG GGAATATGAA CTCCCATCCC CAACCCCTTC ATCTAACGCT
    CCTCCTCCCC CACCTCAGCG AAACACCTCT TACCTCAAAA CACAGGTCCT CTCCCCTGAC
    ACGATTTATA CTGCCAAGTT TGTTGCATAC AATGATGATG AGGAGGAGGA GGATTCCAAT
    CTAGCAGGTC AGGATAAGTA CTCCAGCACA AGAAAATCTT ATGGTGACTT TCCTCCTGCC
    ACCCTTAAAC CACAGCAGCC CTCCCGCCAG CCTCGCCCAG TTTCAGATGG CCTCTTTCTT
    TCCAACTCAT TCCAGTCATC TCCAGTCAAT GCCAACAGTA CTGCTGCCAA AAAAAGCCAG
    CCCCCGCCCC CACCAAACAA ACCGAGTTTC ATCCATGCCA GCAGCTCTAA AGGAATAAAT
    TCATACACTG GATCTTCGGG AGCAACGGTT GGGGCCCATG AAGTCTATCG GGATCCAAGA
    GACAAACGAT CTAAAAGTCA AGATACAGAT TTACCTGGAG CACCAGAAAA TCTGACGTTC
    AGGGAACGCC AGCGACTTTT TTCACAGGGT CAAGATGTAT CCAATAAAGT AAAAGCCTCT
    AGGAAACTAA TGGAACTGGA AAATGAGTTG AACACGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139835.1
    CDS2885..8626
    Translation

    Target ORF information:

    RefSeq Version XM_015284349.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284349.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGTTACAGG ATGAAGAACG CAGACGACAG CAACAGTTAG AAGAAATGCG CAAGCGAGAA
    GCAGAAGACA GGGCCAGGCA GGAAGAGGAG CGCCGACAGC AAGAGGAGGA GCGAACCAGG
    AGAGAGGCAG AAGAAAAGAG GCGACAGGAA GAGGAGTATT ACAATCGCTT AGAGGCTGAA
    AGGCGCAGGC AGCACGATGA AGCAGAACGA AGATTGCTGG AACCAGACGA GCCTGGTCTG
    TACAGACCTC CACTTCCACG GGAATATGAA CTCCCATCCC CAACCCCTTC ATCTAACGCT
    CCTCCTCCCC CACCTCAGCG AAACACCTCT TACCTCAAAA CACAGGTCCT CTCCCCTGAC
    ACGATTTATA CTGCCAAGTT TGTTGCATAC AATGATGATG AGGAGGAGGA GGATTCCAAT
    CTAGCAGGTC AGGATAAGTA CTCCAGCACA AGAAAATCTT ATGGTGACTT TCCTCCTGCC
    ACCCTTAAAC CACAGCAGCC CTCCCGCCAG CCTCGCCCAG TTTCAGATGG CCTCTTTCTT
    TCCAACTCAT TCCAGTCATC TCCAGTCAAT GCCAACAGTA CTGCTGCCAA AAAAAGCCAG
    CCCCCGCCCC CACCAAACAA ACCGAGTTTC ATCCATGCCA GCAGCTCTAA AGGAATAAAT
    TCATACACTG GATCTTCGGG AGCAACGGTT GGGGCCCATG AAGTCTATCG GGATCCAAGA
    GACAAACGAT CTAAAAGTCA AGATACAGAT TTACCTGGAG CACCAGAAAA TCTGACGTTC
    AGGGAACGCC AGCGACTTTT TTCACAGGGT CAAGATGTAT CCAATAAAGT AAAAGCCTCT
    AGGAAACTAA TGGAACTGGA AAATGAGTTG AACACGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33800
    Clone ID Related Accession (Same CDS sequence) XM_015284350.1 , XM_015284350.2
    Accession Version XM_015284350.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGGATGAAG AACGCAGACG ACAGCAACAG TTAGAAGAAA TGCGCAAGCG AGAAGCAGAA
    GACAGGGCCA GGCAGGAAGA GGAGCGCCGA CAGCAAGAGG AGGAGCGAAC CAGGAGAGAG
    GCAGAAGAAA AGAGGCGACA GGAAGAGGAG TATTACAATC GCTTAGAGGC TGAAAGGCGC
    AGGCAGCACG ATGAAGCAGA ACGAAGATTG CTGGAACCAG ACGAGCCTGG TCTGTACAGA
    CCTCCACTTC CACGGGAATA TGAACTCCCA TCCCCAACCC CTTCATCTAA CGCTCCTCCT
    CCCCCACCTC AGCGAAACAC CTCTTACCTC AAAACACAGG TCCTCTCCCC TGACACGATT
    TATACTGCCA AGTTTGTTGC ATACAATGAT GATGAGGAGG AGGAGGATTC CAATCTAGCA
    GGTCAGGATA AGTACTCCAG CACAAGAAAA TCTTATGGTG ACTTTCCTCC TGCCACCCTT
    AAACCACAGC AGCCCTCCCG CCAGCCTCGC CCAGTTTCAG ATGGCCTCTT TCTTTCCAAC
    TCATTCCAGT CATCTCCAGT CAATGCCAAC AGTACTGCTG CCAAAAAAAG CCAGCCCCCG
    CCCCCACCAA ACAAACCGAG TTTCATCCAT GCCAGCAGCT CTAAAGGAAT AAATTCATAC
    ACTGGATCTT CGGGAGCAAC GGTTGGGGCC CATGAAGTCT ATCGGGATCC AAGAGACAAA
    CGATCTAAAA GTCAAGATAC AGATTTACCT GGAGCACCAG AAAATCTGAC GTTCAGGGAA
    CGCCAGCGAC TTTTTTCACA GGGTCAAGAT GTATCCAATA AAGTAAAAGC CTCTAGGAAA
    CTAATGGAAC TGGAAAATGA GTTGAACACG AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139836.1
    CDS33..5768
    Translation

    Target ORF information:

    RefSeq Version XM_015284350.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284350.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGGATGAAG AACGCAGACG ACAGCAACAG TTAGAAGAAA TGCGCAAGCG AGAAGCAGAA
    GACAGGGCCA GGCAGGAAGA GGAGCGCCGA CAGCAAGAGG AGGAGCGAAC CAGGAGAGAG
    GCAGAAGAAA AGAGGCGACA GGAAGAGGAG TATTACAATC GCTTAGAGGC TGAAAGGCGC
    AGGCAGCACG ATGAAGCAGA ACGAAGATTG CTGGAACCAG ACGAGCCTGG TCTGTACAGA
    CCTCCACTTC CACGGGAATA TGAACTCCCA TCCCCAACCC CTTCATCTAA CGCTCCTCCT
    CCCCCACCTC AGCGAAACAC CTCTTACCTC AAAACACAGG TCCTCTCCCC TGACACGATT
    TATACTGCCA AGTTTGTTGC ATACAATGAT GATGAGGAGG AGGAGGATTC CAATCTAGCA
    GGTCAGGATA AGTACTCCAG CACAAGAAAA TCTTATGGTG ACTTTCCTCC TGCCACCCTT
    AAACCACAGC AGCCCTCCCG CCAGCCTCGC CCAGTTTCAG ATGGCCTCTT TCTTTCCAAC
    TCATTCCAGT CATCTCCAGT CAATGCCAAC AGTACTGCTG CCAAAAAAAG CCAGCCCCCG
    CCCCCACCAA ACAAACCGAG TTTCATCCAT GCCAGCAGCT CTAAAGGAAT AAATTCATAC
    ACTGGATCTT CGGGAGCAAC GGTTGGGGCC CATGAAGTCT ATCGGGATCC AAGAGACAAA
    CGATCTAAAA GTCAAGATAC AGATTTACCT GGAGCACCAG AAAATCTGAC GTTCAGGGAA
    CGCCAGCGAC TTTTTTCACA GGGTCAAGAT GTATCCAATA AAGTAAAAGC CTCTAGGAAA
    CTAATGGAAC TGGAAAATGA GTTGAACACG AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33800
    Clone ID Related Accession (Same CDS sequence) XM_015284350.1 , XM_015284350.2
    Accession Version XM_015284350.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284350.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGGATGAAG AACGCAGACG ACAGCAACAG TTAGAAGAAA TGCGCAAGCG AGAAGCAGAA
    GACAGGGCCA GGCAGGAAGA GGAGCGCCGA CAGCAAGAGG AGGAGCGAAC CAGGAGAGAG
    GCAGAAGAAA AGAGGCGACA GGAAGAGGAG TATTACAATC GCTTAGAGGC TGAAAGGCGC
    AGGCAGCACG ATGAAGCAGA ACGAAGATTG CTGGAACCAG ACGAGCCTGG TCTGTACAGA
    CCTCCACTTC CACGGGAATA TGAACTCCCA TCCCCAACCC CTTCATCTAA CGCTCCTCCT
    CCCCCACCTC AGCGAAACAC CTCTTACCTC AAAACACAGG TCCTCTCCCC TGACACGATT
    TATACTGCCA AGTTTGTTGC ATACAATGAT GATGAGGAGG AGGAGGATTC CAATCTAGCA
    GGTCAGGATA AGTACTCCAG CACAAGAAAA TCTTATGGTG ACTTTCCTCC TGCCACCCTT
    AAACCACAGC AGCCCTCCCG CCAGCCTCGC CCAGTTTCAG ATGGCCTCTT TCTTTCCAAC
    TCATTCCAGT CATCTCCAGT CAATGCCAAC AGTACTGCTG CCAAAAAAAG CCAGCCCCCG
    CCCCCACCAA ACAAACCGAG TTTCATCCAT GCCAGCAGCT CTAAAGGAAT AAATTCATAC
    ACTGGATCTT CGGGAGCAAC GGTTGGGGCC CATGAAGTCT ATCGGGATCC AAGAGACAAA
    CGATCTAAAA GTCAAGATAC AGATTTACCT GGAGCACCAG AAAATCTGAC GTTCAGGGAA
    CGCCAGCGAC TTTTTTCACA GGGTCAAGAT GTATCCAATA AAGTAAAAGC CTCTAGGAAA
    CTAATGGAAC TGGAAAATGA GTTGAACACG AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139836.1
    CDS2885..8620
    Translation

    Target ORF information:

    RefSeq Version XM_015284350.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284350.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGGATGAAG AACGCAGACG ACAGCAACAG TTAGAAGAAA TGCGCAAGCG AGAAGCAGAA
    GACAGGGCCA GGCAGGAAGA GGAGCGCCGA CAGCAAGAGG AGGAGCGAAC CAGGAGAGAG
    GCAGAAGAAA AGAGGCGACA GGAAGAGGAG TATTACAATC GCTTAGAGGC TGAAAGGCGC
    AGGCAGCACG ATGAAGCAGA ACGAAGATTG CTGGAACCAG ACGAGCCTGG TCTGTACAGA
    CCTCCACTTC CACGGGAATA TGAACTCCCA TCCCCAACCC CTTCATCTAA CGCTCCTCCT
    CCCCCACCTC AGCGAAACAC CTCTTACCTC AAAACACAGG TCCTCTCCCC TGACACGATT
    TATACTGCCA AGTTTGTTGC ATACAATGAT GATGAGGAGG AGGAGGATTC CAATCTAGCA
    GGTCAGGATA AGTACTCCAG CACAAGAAAA TCTTATGGTG ACTTTCCTCC TGCCACCCTT
    AAACCACAGC AGCCCTCCCG CCAGCCTCGC CCAGTTTCAG ATGGCCTCTT TCTTTCCAAC
    TCATTCCAGT CATCTCCAGT CAATGCCAAC AGTACTGCTG CCAAAAAAAG CCAGCCCCCG
    CCCCCACCAA ACAAACCGAG TTTCATCCAT GCCAGCAGCT CTAAAGGAAT AAATTCATAC
    ACTGGATCTT CGGGAGCAAC GGTTGGGGCC CATGAAGTCT ATCGGGATCC AAGAGACAAA
    CGATCTAAAA GTCAAGATAC AGATTTACCT GGAGCACCAG AAAATCTGAC GTTCAGGGAA
    CGCCAGCGAC TTTTTTCACA GGGTCAAGAT GTATCCAATA AAGTAAAAGC CTCTAGGAAA
    CTAATGGAAC TGGAAAATGA GTTGAACACG AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33801
    Clone ID Related Accession (Same CDS sequence) XM_015284351.2 , XM_015284351.1
    Accession Version XM_015284351.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5733bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284351.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGATGGTTC TGACTCCAGA
    GATAAACCAA AGCTTTATCG TCTACAATTA AGTGTCACTG AAGTTGGAAC TGAAAAATTT
    GATGACAATT CCATTCAGTT ATTTGGTCCA GGAATTCAGC CTCATCATTG TGATCTTACT
    AATATGGATG GAGTTGTTAC TGTAACCCCA AGAAACATTG ATGCGGAAAC GTATGTAGAA
    GGTCAGCGTA TTTCAGAAAC CACTATGCTG CAAAGTGGAA TGAAGGTTCA GTTTGGGTCA
    TCTCATGTGT TTAAGTTTGT GGATCCCACT CAGGACCATA TTATTACTAA AAGACCTATT
    GATGCAAGCC TTATGGTCAA AGGTCCAAGG CATAAAGCTG GAGCTGTTCA GGAAACCACG
    TTTGATCTGG AGGGAGATGT TCACAGTGGA ACAGCGTTAC CAGCGAGCAA GAGCACCAGC
    AGGCTTGATG GTGACAGAAC ATCATCAGCA TCCAGCACAG CTGAACGAGG AATGGTGAAG
    CCCATGATTA GAATAGAACA GCAGCAAGAT TATCGCAGAC AGGAAAGCAG ACCTCAGGAT
    ACCCATGGAC CAGAACTGAT CCTACCTGCC AGTATTGAAT TCAGGGAAAG CTCTGAAGAT
    GCCTTCTTAT CTGCCATTAT AAATTATACA AATAGCTCGA CAGTTCATTT TAAACTGTCA
    CCCACGTATG TTTTGTATAT GACATGTCGG TATGTATTGT CAAGCCAGTA CAGACCTGAC
    ATCACTCCTA CTGAGCGTAC TCACAAAGTT ATTGCAATAG TCAACAAGAT GGTGAATATG
    ATGGAAGGAG TCATACAGGA GGTAGACCAG GTTGATCAGA AACAGAAGAA TATTGCTGGG
    GCACTTGCCT TTTGGATGGC AAATGCGTCT GAATTGCTGA ATTTTATAAA GCAAGACCGT
    GATCTGAGCC GAATCACTGT GGATGCTCAA GATGTTTTGG CGCACTTGGT TCAAATGGCA
    TTCAAGTACC TAGTTCATTG TCTGCAGTCA GAGCTTAATA ACTACATGCC AGCATTTCTT
    GATGATCCAG AGGAAAATAA TCCACAGAGG CCTAAAATAG ATGATGTGCT GCACACATTG
    ACTGGAGCTA TGTCCTTGTT GCGACGATGT AGAGTGAATG CTGCACTGAC TATACAGCTC
    TTCTCCCAGT TGTTTCATTT TATCAACATG TGGCTTTTTA ACAGACTAGT TACGGCTCCA
    GATTCAGGGT TATGTTCCCA CTACTGGGGA GCTATTGTTC GTCAGCAGTT GGGTCATATT
    GAGGCCTGGG CAGAAAAACA GGGTTTAGAA TTGGCTGCTG ATTGCCACTT AAGCAGAATA
    GTGCAGGCAA CCACATTGCT CACCATGGAC AAGTACTCAC ATGCTGATGT TCCAAATATT
    AACAGTACTT GCTTTAAGTT GAATTCTCTA CAGCTACAAG CTTTGTTGCA GAATTATCAC
    TGTGCTCCAG ATGAACCGCT TATACCAACA GAACTGATAG AGAATGTAGT AACTGTTGCA
    GAAAATACGG CTGATGAACT TGCTCGCAGT GATGGCAGAG AAGTCCAATT GGAAGAGGAT
    CCTGACTTAC AACTCCCTTT TCTTTTACCA GAAGATGGCT ATTCCTGTGA TGTTGTCAGA
    AATGTTCCAA GTGGTTTACA AGAATTTTTG GATCCCCTCT GTCAGAGAGG TTTTTGTAGG
    TTAATACCTC ATACACGGTC ACCAGGCACA TGGACAATAT ACTTTGAAGG CGCAGACTAT
    GAAAGCCACC TGTCACATGA GAATGCTGAG CTAGCTCAGC CTTTGAGGAA AGAACCTGAG
    ATTATAACTG TAACACTAAA AAAACAAAAT GGAATGGGCC TCAGCATTGT TGCTGCCAAG
    GGTGCTGGTC AAGACAAACT TGGAATATAT GTCAAGTCTG TTGTGAAAGG AGGTGCTGCA
    GATGTGGATG GTCGTCTGGC AGCAGGGGAT CAGCTGCTCA GTGTGGATGG TCGAAGCTTG
    GTGGGGCTGT CCCAGGAAAG GGCAGCAGAA CTTATGACGA GGACAGGTTC TGTTGTAACA
    CTGGAAGTAG CAAAACAAGG AGCAATATAC CACGGTCTGG CAACCCTGCT AAATCAGCCA
    TCTCCAATGA TGCAAAGAGT TTCTGACCGC CGTGGCTCAG GTAAACCCCG ACCAAAGAGT
    GAAGGTTTTG AGCTGTATAA CAACTCCACG CAAAATGGGT CACCTGAGAG CCCCCAGCTG
    CCATGGACGG AGTACAGTGA ACCAAAGAAG CTGCCAGGAG ATGACAGATT AATGAAGAAC
    AGAGCCGATC ATCGCTCCAG CCCCAACGTA GCAAATCAGC CTCCAAGCCC TGGGGGGAAG
    AATGCTTATG CATCTGGAAC TACCACCAAA ATAACCTCAG TCTCGACTGG AAATCTTTGT
    ACAGAGGAGC AAACTCTGCC TCCACGACCT GAAGCTTATC CTATCCCAAC ACAGACCTAT
    ACCAGAGAAT ATTTCACATT CCCAGCTTCA AAATCCCAGG ATCGAATGGG TCCAGCTCAG
    AGTCAGTGGC CAAATTATGA AGAGAAGCCA CAGATCCAGG CAGAAAGTAA TCACTCAATC
    AATACTACAA TGCAGCGCGT GGCTCGATCC CAGGAAGAAC TCCGAGATAA AGTTTTCCAG
    CCAGAGAGGA ATCGTTTGGA AGTTGTTATC CGGAAGGACA TAGAATACAT TGATCGGAAG
    TCAGACAGCG ATCAGTGGAT GAATTCATCT GTGGATTCTA GCACATCTAG CCAAGAGCAC
    CTGAACCATA CATCCAAACC TGTTTCACCT GGTTCTTGCT TAACTAAAAG TGGACCTGGC
    CGTTGGAAAA CTCCAGCTAC GAGCCAGCCA ACTCCAGTGG CCGTTTCCCA GCCCATCAGA
    ACAGACCTTC CTCCTCCGCC GCCACCTCCT CCGGTGCATT ATGCAGCTGA ATTTGATGAA
    CCACAAATGG ATTTGCCTCT GCCTCCCCCT CCTCCACCGA ACCAAGTAGG AGCACAAGCA
    TCTTCTCAGG TGGCTGCTGC TGAGCGTAAA AAACGAGAAG AGCATCAGAG GTGGTATGAA
    AAGGAAAAGG CACGTTTGGA AGAAGAACGA GAAAGGAAAC GCCGTGAGCA AGAGAGAAAA
    CTGGGCCAAA TGCGTTCGCA GCCACTAATT CCTCCCCAGC CTGTGGTTCC TCCTGTTTCC
    CTTCAGCAAG TGAAATCGGA AAAAACTTCA ACTTTCCAGA GGTCTCAAGA AACAGTCATT
    CGAGAGTTGC AGCCGCAACA GCAGCCTCGG ACTATTGAGC GGAGAGATCT GCAGTACATT
    ACTATCAGCA AGGAAGAATT GTCCTCTAGT GACAGCCTCT CTCCAGATCC CTGGAAACGA
    GATGCCAAGG AGAAACTGGA GAAGCAGCAG CAGATGCACA TTGTGGACAT GCTGAGCAAA
    GAGATTCAGG ATCTTCAGAA CAAGCCTGAT CGTACAGCTG AAGAGAACGA CCGCCTGCGC
    AAACTCATGC TAGAGTGGCA GTTCCAAAAG CGACTTCAGG AATCAAAACA AAAGGATGAA
    GATGATGATG AAGAAGAGGA CGATGATGTG GATACTATGC TTATCATGCA GCGGCTGGAG
    GCAGAAAGAA GAGCAAGGCA GACTGCTGTG CCAGCAATCT CTGTTCTAGA CTTGTTACAG
    GATGAAGAAC GCAGACGACA GCAACAGTTA GAAGAAATGC GCAAGCGAGA AGCAGAAGAC
    AGGGCCAGGC AGGAAGAGGA GCGCCGACAG CAAGAGGAGG AGCGAACCAG GAGAGAGGCA
    GAAGAAAAGA GGCGACAGGA AGAGGAGTAT TACAATCGCT TAGAGGCTGA AAGGCGCAGG
    CAGCACGATG AAGCAGAACG AAGATTGCTG GAACCAGACG AGCCTGGTCT GTACAGACCT
    CCACTTCCAC GGGAATATGA ACTCCCATCC CCAACCCCTT CATCTAACGC TCCTCCTCCC
    CCACCTCAGC GAAACACCTC TTACCTCAAA ACACAGGTCC TCTCCCCTGA CACGATTTAT
    ACTGCCAAGT TTGTTGCATA CAATGATGAT GAGGAGGAGG AGGATTCCAA TCTAGCAGGT
    CAGGATAAGT ACTCCAGCAC AAGAAAATCT TATGGTGACT TTCCTCCTGC CACCCTTAAA
    CCACAGCAGC CCTCCCGCCA GCCTCGCCCA GTTTCAGATG GCCTCTTTCT TTCCAACTCA
    TTCCAGTCAT CTCCAGTCAA TGCCAACAGT ACTGCTGCCA AAAAAAGCCA GCCCCCGCCC
    CCACCAAACA AACCGAGTTT CATCCATGCC AGCAGCTCTA AAGGAATAAA TTCATACACT
    GGATCTTCGG GAGCAACGGT TGGGGCCCAT GAAGTCTATC GGGATCCAAG AGACAAACGA
    TCTAAAAGTC AAGATACAGA TTTACCTGGA GCACCAGAAA ATCTGACGTT CAGGGAACGC
    CAGCGACTTT TTTCACAGGG TCAAGATGTA TCCAATAAAG TAAAAGCCTC TAGGAAACTA
    ATGGAACTGG AAAATGAGTT GAACACGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139837.1
    CDS2885..8617
    Translation

    Target ORF information:

    RefSeq Version XM_015284351.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284351.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGATGGTTC TGACTCCAGA
    GATAAACCAA AGCTTTATCG TCTACAATTA AGTGTCACTG AAGTTGGAAC TGAAAAATTT
    GATGACAATT CCATTCAGTT ATTTGGTCCA GGAATTCAGC CTCATCATTG TGATCTTACT
    AATATGGATG GAGTTGTTAC TGTAACCCCA AGAAACATTG ATGCGGAAAC GTATGTAGAA
    GGTCAGCGTA TTTCAGAAAC CACTATGCTG CAAAGTGGAA TGAAGGTTCA GTTTGGGTCA
    TCTCATGTGT TTAAGTTTGT GGATCCCACT CAGGACCATA TTATTACTAA AAGACCTATT
    GATGCAAGCC TTATGGTCAA AGGTCCAAGG CATAAAGCTG GAGCTGTTCA GGAAACCACG
    TTTGATCTGG AGGGAGATGT TCACAGTGGA ACAGCGTTAC CAGCGAGCAA GAGCACCAGC
    AGGCTTGATG GTGACAGAAC ATCATCAGCA TCCAGCACAG CTGAACGAGG AATGGTGAAG
    CCCATGATTA GAATAGAACA GCAGCAAGAT TATCGCAGAC AGGAAAGCAG ACCTCAGGAT
    ACCCATGGAC CAGAACTGAT CCTACCTGCC AGTATTGAAT TCAGGGAAAG CTCTGAAGAT
    GCCTTCTTAT CTGCCATTAT AAATTATACA AATAGCTCGA CAGTTCATTT TAAACTGTCA
    CCCACGTATG TTTTGTATAT GACATGTCGG TATGTATTGT CAAGCCAGTA CAGACCTGAC
    ATCACTCCTA CTGAGCGTAC TCACAAAGTT ATTGCAATAG TCAACAAGAT GGTGAATATG
    ATGGAAGGAG TCATACAGGA GGTAGACCAG GTTGATCAGA AACAGAAGAA TATTGCTGGG
    GCACTTGCCT TTTGGATGGC AAATGCGTCT GAATTGCTGA ATTTTATAAA GCAAGACCGT
    GATCTGAGCC GAATCACTGT GGATGCTCAA GATGTTTTGG CGCACTTGGT TCAAATGGCA
    TTCAAGTACC TAGTTCATTG TCTGCAGTCA GAGCTTAATA ACTACATGCC AGCATTTCTT
    GATGATCCAG AGGAAAATAA TCCACAGAGG CCTAAAATAG ATGATGTGCT GCACACATTG
    ACTGGAGCTA TGTCCTTGTT GCGACGATGT AGAGTGAATG CTGCACTGAC TATACAGCTC
    TTCTCCCAGT TGTTTCATTT TATCAACATG TGGCTTTTTA ACAGACTAGT TACGGCTCCA
    GATTCAGGGT TATGTTCCCA CTACTGGGGA GCTATTGTTC GTCAGCAGTT GGGTCATATT
    GAGGCCTGGG CAGAAAAACA GGGTTTAGAA TTGGCTGCTG ATTGCCACTT AAGCAGAATA
    GTGCAGGCAA CCACATTGCT CACCATGGAC AAGTACTCAC ATGCTGATGT TCCAAATATT
    AACAGTACTT GCTTTAAGTT GAATTCTCTA CAGCTACAAG CTTTGTTGCA GAATTATCAC
    TGTGCTCCAG ATGAACCGCT TATACCAACA GAACTGATAG AGAATGTAGT AACTGTTGCA
    GAAAATACGG CTGATGAACT TGCTCGCAGT GATGGCAGAG AAGTCCAATT GGAAGAGGAT
    CCTGACTTAC AACTCCCTTT TCTTTTACCA GAAGATGGCT ATTCCTGTGA TGTTGTCAGA
    AATGTTCCAA GTGGTTTACA AGAATTTTTG GATCCCCTCT GTCAGAGAGG TTTTTGTAGG
    TTAATACCTC ATACACGGTC ACCAGGCACA TGGACAATAT ACTTTGAAGG CGCAGACTAT
    GAAAGCCACC TGTCACATGA GAATGCTGAG CTAGCTCAGC CTTTGAGGAA AGAACCTGAG
    ATTATAACTG TAACACTAAA AAAACAAAAT GGAATGGGCC TCAGCATTGT TGCTGCCAAG
    GGTGCTGGTC AAGACAAACT TGGAATATAT GTCAAGTCTG TTGTGAAAGG AGGTGCTGCA
    GATGTGGATG GTCGTCTGGC AGCAGGGGAT CAGCTGCTCA GTGTGGATGG TCGAAGCTTG
    GTGGGGCTGT CCCAGGAAAG GGCAGCAGAA CTTATGACGA GGACAGGTTC TGTTGTAACA
    CTGGAAGTAG CAAAACAAGG AGCAATATAC CACGGTCTGG CAACCCTGCT AAATCAGCCA
    TCTCCAATGA TGCAAAGAGT TTCTGACCGC CGTGGCTCAG GTAAACCCCG ACCAAAGAGT
    GAAGGTTTTG AGCTGTATAA CAACTCCACG CAAAATGGGT CACCTGAGAG CCCCCAGCTG
    CCATGGACGG AGTACAGTGA ACCAAAGAAG CTGCCAGGAG ATGACAGATT AATGAAGAAC
    AGAGCCGATC ATCGCTCCAG CCCCAACGTA GCAAATCAGC CTCCAAGCCC TGGGGGGAAG
    AATGCTTATG CATCTGGAAC TACCACCAAA ATAACCTCAG TCTCGACTGG AAATCTTTGT
    ACAGAGGAGC AAACTCTGCC TCCACGACCT GAAGCTTATC CTATCCCAAC ACAGACCTAT
    ACCAGAGAAT ATTTCACATT CCCAGCTTCA AAATCCCAGG ATCGAATGGG TCCAGCTCAG
    AGTCAGTGGC CAAATTATGA AGAGAAGCCA CAGATCCAGG CAGAAAGTAA TCACTCAATC
    AATACTACAA TGCAGCGCGT GGCTCGATCC CAGGAAGAAC TCCGAGATAA AGTTTTCCAG
    CCAGAGAGGA ATCGTTTGGA AGTTGTTATC CGGAAGGACA TAGAATACAT TGATCGGAAG
    TCAGACAGCG ATCAGTGGAT GAATTCATCT GTGGATTCTA GCACATCTAG CCAAGAGCAC
    CTGAACCATA CATCCAAACC TGTTTCACCT GGTTCTTGCT TAACTAAAAG TGGACCTGGC
    CGTTGGAAAA CTCCAGCTAC GAGCCAGCCA ACTCCAGTGG CCGTTTCCCA GCCCATCAGA
    ACAGACCTTC CTCCTCCGCC GCCACCTCCT CCGGTGCATT ATGCAGCTGA ATTTGATGAA
    CCACAAATGG ATTTGCCTCT GCCTCCCCCT CCTCCACCGA ACCAAGTAGG AGCACAAGCA
    TCTTCTCAGG TGGCTGCTGC TGAGCGTAAA AAACGAGAAG AGCATCAGAG GTGGTATGAA
    AAGGAAAAGG CACGTTTGGA AGAAGAACGA GAAAGGAAAC GCCGTGAGCA AGAGAGAAAA
    CTGGGCCAAA TGCGTTCGCA GCCACTAATT CCTCCCCAGC CTGTGGTTCC TCCTGTTTCC
    CTTCAGCAAG TGAAATCGGA AAAAACTTCA ACTTTCCAGA GGTCTCAAGA AACAGTCATT
    CGAGAGTTGC AGCCGCAACA GCAGCCTCGG ACTATTGAGC GGAGAGATCT GCAGTACATT
    ACTATCAGCA AGGAAGAATT GTCCTCTAGT GACAGCCTCT CTCCAGATCC CTGGAAACGA
    GATGCCAAGG AGAAACTGGA GAAGCAGCAG CAGATGCACA TTGTGGACAT GCTGAGCAAA
    GAGATTCAGG ATCTTCAGAA CAAGCCTGAT CGTACAGCTG AAGAGAACGA CCGCCTGCGC
    AAACTCATGC TAGAGTGGCA GTTCCAAAAG CGACTTCAGG AATCAAAACA AAAGGATGAA
    GATGATGATG AAGAAGAGGA CGATGATGTG GATACTATGC TTATCATGCA GCGGCTGGAG
    GCAGAAAGAA GAGCAAGGCA GACTGCTGTG CCAGCAATCT CTGTTCTAGA CTTGTTACAG
    GATGAAGAAC GCAGACGACA GCAACAGTTA GAAGAAATGC GCAAGCGAGA AGCAGAAGAC
    AGGGCCAGGC AGGAAGAGGA GCGCCGACAG CAAGAGGAGG AGCGAACCAG GAGAGAGGCA
    GAAGAAAAGA GGCGACAGGA AGAGGAGTAT TACAATCGCT TAGAGGCTGA AAGGCGCAGG
    CAGCACGATG AAGCAGAACG AAGATTGCTG GAACCAGACG AGCCTGGTCT GTACAGACCT
    CCACTTCCAC GGGAATATGA ACTCCCATCC CCAACCCCTT CATCTAACGC TCCTCCTCCC
    CCACCTCAGC GAAACACCTC TTACCTCAAA ACACAGGTCC TCTCCCCTGA CACGATTTAT
    ACTGCCAAGT TTGTTGCATA CAATGATGAT GAGGAGGAGG AGGATTCCAA TCTAGCAGGT
    CAGGATAAGT ACTCCAGCAC AAGAAAATCT TATGGTGACT TTCCTCCTGC CACCCTTAAA
    CCACAGCAGC CCTCCCGCCA GCCTCGCCCA GTTTCAGATG GCCTCTTTCT TTCCAACTCA
    TTCCAGTCAT CTCCAGTCAA TGCCAACAGT ACTGCTGCCA AAAAAAGCCA GCCCCCGCCC
    CCACCAAACA AACCGAGTTT CATCCATGCC AGCAGCTCTA AAGGAATAAA TTCATACACT
    GGATCTTCGG GAGCAACGGT TGGGGCCCAT GAAGTCTATC GGGATCCAAG AGACAAACGA
    TCTAAAAGTC AAGATACAGA TTTACCTGGA GCACCAGAAA ATCTGACGTT CAGGGAACGC
    CAGCGACTTT TTTCACAGGG TCAAGATGTA TCCAATAAAG TAAAAGCCTC TAGGAAACTA
    ATGGAACTGG AAAATGAGTT GAACACGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33801
    Clone ID Related Accession (Same CDS sequence) XM_015284351.2 , XM_015284351.1
    Accession Version XM_015284351.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5733bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGATGGTTC TGACTCCAGA
    GATAAACCAA AGCTTTATCG TCTACAATTA AGTGTCACTG AAGTTGGAAC TGAAAAATTT
    GATGACAATT CCATTCAGTT ATTTGGTCCA GGAATTCAGC CTCATCATTG TGATCTTACT
    AATATGGATG GAGTTGTTAC TGTAACCCCA AGAAACATTG ATGCGGAAAC GTATGTAGAA
    GGTCAGCGTA TTTCAGAAAC CACTATGCTG CAAAGTGGAA TGAAGGTTCA GTTTGGGTCA
    TCTCATGTGT TTAAGTTTGT GGATCCCACT CAGGACCATA TTATTACTAA AAGACCTATT
    GATGCAAGCC TTATGGTCAA AGGTCCAAGG CATAAAGCTG GAGCTGTTCA GGAAACCACG
    TTTGATCTGG AGGGAGATGT TCACAGTGGA ACAGCGTTAC CAGCGAGCAA GAGCACCAGC
    AGGCTTGATG GTGACAGAAC ATCATCAGCA TCCAGCACAG CTGAACGAGG AATGGTGAAG
    CCCATGATTA GAATAGAACA GCAGCAAGAT TATCGCAGAC AGGAAAGCAG ACCTCAGGAT
    ACCCATGGAC CAGAACTGAT CCTACCTGCC AGTATTGAAT TCAGGGAAAG CTCTGAAGAT
    GCCTTCTTAT CTGCCATTAT AAATTATACA AATAGCTCGA CAGTTCATTT TAAACTGTCA
    CCCACGTATG TTTTGTATAT GACATGTCGG TATGTATTGT CAAGCCAGTA CAGACCTGAC
    ATCACTCCTA CTGAGCGTAC TCACAAAGTT ATTGCAATAG TCAACAAGAT GGTGAATATG
    ATGGAAGGAG TCATACAGGA GGTAGACCAG GTTGATCAGA AACAGAAGAA TATTGCTGGG
    GCACTTGCCT TTTGGATGGC AAATGCGTCT GAATTGCTGA ATTTTATAAA GCAAGACCGT
    GATCTGAGCC GAATCACTGT GGATGCTCAA GATGTTTTGG CGCACTTGGT TCAAATGGCA
    TTCAAGTACC TAGTTCATTG TCTGCAGTCA GAGCTTAATA ACTACATGCC AGCATTTCTT
    GATGATCCAG AGGAAAATAA TCCACAGAGG CCTAAAATAG ATGATGTGCT GCACACATTG
    ACTGGAGCTA TGTCCTTGTT GCGACGATGT AGAGTGAATG CTGCACTGAC TATACAGCTC
    TTCTCCCAGT TGTTTCATTT TATCAACATG TGGCTTTTTA ACAGACTAGT TACGGCTCCA
    GATTCAGGGT TATGTTCCCA CTACTGGGGA GCTATTGTTC GTCAGCAGTT GGGTCATATT
    GAGGCCTGGG CAGAAAAACA GGGTTTAGAA TTGGCTGCTG ATTGCCACTT AAGCAGAATA
    GTGCAGGCAA CCACATTGCT CACCATGGAC AAGTACTCAC ATGCTGATGT TCCAAATATT
    AACAGTACTT GCTTTAAGTT GAATTCTCTA CAGCTACAAG CTTTGTTGCA GAATTATCAC
    TGTGCTCCAG ATGAACCGCT TATACCAACA GAACTGATAG AGAATGTAGT AACTGTTGCA
    GAAAATACGG CTGATGAACT TGCTCGCAGT GATGGCAGAG AAGTCCAATT GGAAGAGGAT
    CCTGACTTAC AACTCCCTTT TCTTTTACCA GAAGATGGCT ATTCCTGTGA TGTTGTCAGA
    AATGTTCCAA GTGGTTTACA AGAATTTTTG GATCCCCTCT GTCAGAGAGG TTTTTGTAGG
    TTAATACCTC ATACACGGTC ACCAGGCACA TGGACAATAT ACTTTGAAGG CGCAGACTAT
    GAAAGCCACC TGTCACATGA GAATGCTGAG CTAGCTCAGC CTTTGAGGAA AGAACCTGAG
    ATTATAACTG TAACACTAAA AAAACAAAAT GGAATGGGCC TCAGCATTGT TGCTGCCAAG
    GGTGCTGGTC AAGACAAACT TGGAATATAT GTCAAGTCTG TTGTGAAAGG AGGTGCTGCA
    GATGTGGATG GTCGTCTGGC AGCAGGGGAT CAGCTGCTCA GTGTGGATGG TCGAAGCTTG
    GTGGGGCTGT CCCAGGAAAG GGCAGCAGAA CTTATGACGA GGACAGGTTC TGTTGTAACA
    CTGGAAGTAG CAAAACAAGG AGCAATATAC CACGGTCTGG CAACCCTGCT AAATCAGCCA
    TCTCCAATGA TGCAAAGAGT TTCTGACCGC CGTGGCTCAG GTAAACCCCG ACCAAAGAGT
    GAAGGTTTTG AGCTGTATAA CAACTCCACG CAAAATGGGT CACCTGAGAG CCCCCAGCTG
    CCATGGACGG AGTACAGTGA ACCAAAGAAG CTGCCAGGAG ATGACAGATT AATGAAGAAC
    AGAGCCGATC ATCGCTCCAG CCCCAACGTA GCAAATCAGC CTCCAAGCCC TGGGGGGAAG
    AATGCTTATG CATCTGGAAC TACCACCAAA ATAACCTCAG TCTCGACTGG AAATCTTTGT
    ACAGAGGAGC AAACTCTGCC TCCACGACCT GAAGCTTATC CTATCCCAAC ACAGACCTAT
    ACCAGAGAAT ATTTCACATT CCCAGCTTCA AAATCCCAGG ATCGAATGGG TCCAGCTCAG
    AGTCAGTGGC CAAATTATGA AGAGAAGCCA CAGATCCAGG CAGAAAGTAA TCACTCAATC
    AATACTACAA TGCAGCGCGT GGCTCGATCC CAGGAAGAAC TCCGAGATAA AGTTTTCCAG
    CCAGAGAGGA ATCGTTTGGA AGTTGTTATC CGGAAGGACA TAGAATACAT TGATCGGAAG
    TCAGACAGCG ATCAGTGGAT GAATTCATCT GTGGATTCTA GCACATCTAG CCAAGAGCAC
    CTGAACCATA CATCCAAACC TGTTTCACCT GGTTCTTGCT TAACTAAAAG TGGACCTGGC
    CGTTGGAAAA CTCCAGCTAC GAGCCAGCCA ACTCCAGTGG CCGTTTCCCA GCCCATCAGA
    ACAGACCTTC CTCCTCCGCC GCCACCTCCT CCGGTGCATT ATGCAGCTGA ATTTGATGAA
    CCACAAATGG ATTTGCCTCT GCCTCCCCCT CCTCCACCGA ACCAAGTAGG AGCACAAGCA
    TCTTCTCAGG TGGCTGCTGC TGAGCGTAAA AAACGAGAAG AGCATCAGAG GTGGTATGAA
    AAGGAAAAGG CACGTTTGGA AGAAGAACGA GAAAGGAAAC GCCGTGAGCA AGAGAGAAAA
    CTGGGCCAAA TGCGTTCGCA GCCACTAATT CCTCCCCAGC CTGTGGTTCC TCCTGTTTCC
    CTTCAGCAAG TGAAATCGGA AAAAACTTCA ACTTTCCAGA GGTCTCAAGA AACAGTCATT
    CGAGAGTTGC AGCCGCAACA GCAGCCTCGG ACTATTGAGC GGAGAGATCT GCAGTACATT
    ACTATCAGCA AGGAAGAATT GTCCTCTAGT GACAGCCTCT CTCCAGATCC CTGGAAACGA
    GATGCCAAGG AGAAACTGGA GAAGCAGCAG CAGATGCACA TTGTGGACAT GCTGAGCAAA
    GAGATTCAGG ATCTTCAGAA CAAGCCTGAT CGTACAGCTG AAGAGAACGA CCGCCTGCGC
    AAACTCATGC TAGAGTGGCA GTTCCAAAAG CGACTTCAGG AATCAAAACA AAAGGATGAA
    GATGATGATG AAGAAGAGGA CGATGATGTG GATACTATGC TTATCATGCA GCGGCTGGAG
    GCAGAAAGAA GAGCAAGGCA GACTGCTGTG CCAGCAATCT CTGTTCTAGA CTTGTTACAG
    GATGAAGAAC GCAGACGACA GCAACAGTTA GAAGAAATGC GCAAGCGAGA AGCAGAAGAC
    AGGGCCAGGC AGGAAGAGGA GCGCCGACAG CAAGAGGAGG AGCGAACCAG GAGAGAGGCA
    GAAGAAAAGA GGCGACAGGA AGAGGAGTAT TACAATCGCT TAGAGGCTGA AAGGCGCAGG
    CAGCACGATG AAGCAGAACG AAGATTGCTG GAACCAGACG AGCCTGGTCT GTACAGACCT
    CCACTTCCAC GGGAATATGA ACTCCCATCC CCAACCCCTT CATCTAACGC TCCTCCTCCC
    CCACCTCAGC GAAACACCTC TTACCTCAAA ACACAGGTCC TCTCCCCTGA CACGATTTAT
    ACTGCCAAGT TTGTTGCATA CAATGATGAT GAGGAGGAGG AGGATTCCAA TCTAGCAGGT
    CAGGATAAGT ACTCCAGCAC AAGAAAATCT TATGGTGACT TTCCTCCTGC CACCCTTAAA
    CCACAGCAGC CCTCCCGCCA GCCTCGCCCA GTTTCAGATG GCCTCTTTCT TTCCAACTCA
    TTCCAGTCAT CTCCAGTCAA TGCCAACAGT ACTGCTGCCA AAAAAAGCCA GCCCCCGCCC
    CCACCAAACA AACCGAGTTT CATCCATGCC AGCAGCTCTA AAGGAATAAA TTCATACACT
    GGATCTTCGG GAGCAACGGT TGGGGCCCAT GAAGTCTATC GGGATCCAAG AGACAAACGA
    TCTAAAAGTC AAGATACAGA TTTACCTGGA GCACCAGAAA ATCTGACGTT CAGGGAACGC
    CAGCGACTTT TTTCACAGGG TCAAGATGTA TCCAATAAAG TAAAAGCCTC TAGGAAACTA
    ATGGAACTGG AAAATGAGTT GAACACGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139837.1
    CDS33..5765
    Translation

    Target ORF information:

    RefSeq Version XM_015284351.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284351.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGATGGTTC TGACTCCAGA
    GATAAACCAA AGCTTTATCG TCTACAATTA AGTGTCACTG AAGTTGGAAC TGAAAAATTT
    GATGACAATT CCATTCAGTT ATTTGGTCCA GGAATTCAGC CTCATCATTG TGATCTTACT
    AATATGGATG GAGTTGTTAC TGTAACCCCA AGAAACATTG ATGCGGAAAC GTATGTAGAA
    GGTCAGCGTA TTTCAGAAAC CACTATGCTG CAAAGTGGAA TGAAGGTTCA GTTTGGGTCA
    TCTCATGTGT TTAAGTTTGT GGATCCCACT CAGGACCATA TTATTACTAA AAGACCTATT
    GATGCAAGCC TTATGGTCAA AGGTCCAAGG CATAAAGCTG GAGCTGTTCA GGAAACCACG
    TTTGATCTGG AGGGAGATGT TCACAGTGGA ACAGCGTTAC CAGCGAGCAA GAGCACCAGC
    AGGCTTGATG GTGACAGAAC ATCATCAGCA TCCAGCACAG CTGAACGAGG AATGGTGAAG
    CCCATGATTA GAATAGAACA GCAGCAAGAT TATCGCAGAC AGGAAAGCAG ACCTCAGGAT
    ACCCATGGAC CAGAACTGAT CCTACCTGCC AGTATTGAAT TCAGGGAAAG CTCTGAAGAT
    GCCTTCTTAT CTGCCATTAT AAATTATACA AATAGCTCGA CAGTTCATTT TAAACTGTCA
    CCCACGTATG TTTTGTATAT GACATGTCGG TATGTATTGT CAAGCCAGTA CAGACCTGAC
    ATCACTCCTA CTGAGCGTAC TCACAAAGTT ATTGCAATAG TCAACAAGAT GGTGAATATG
    ATGGAAGGAG TCATACAGGA GGTAGACCAG GTTGATCAGA AACAGAAGAA TATTGCTGGG
    GCACTTGCCT TTTGGATGGC AAATGCGTCT GAATTGCTGA ATTTTATAAA GCAAGACCGT
    GATCTGAGCC GAATCACTGT GGATGCTCAA GATGTTTTGG CGCACTTGGT TCAAATGGCA
    TTCAAGTACC TAGTTCATTG TCTGCAGTCA GAGCTTAATA ACTACATGCC AGCATTTCTT
    GATGATCCAG AGGAAAATAA TCCACAGAGG CCTAAAATAG ATGATGTGCT GCACACATTG
    ACTGGAGCTA TGTCCTTGTT GCGACGATGT AGAGTGAATG CTGCACTGAC TATACAGCTC
    TTCTCCCAGT TGTTTCATTT TATCAACATG TGGCTTTTTA ACAGACTAGT TACGGCTCCA
    GATTCAGGGT TATGTTCCCA CTACTGGGGA GCTATTGTTC GTCAGCAGTT GGGTCATATT
    GAGGCCTGGG CAGAAAAACA GGGTTTAGAA TTGGCTGCTG ATTGCCACTT AAGCAGAATA
    GTGCAGGCAA CCACATTGCT CACCATGGAC AAGTACTCAC ATGCTGATGT TCCAAATATT
    AACAGTACTT GCTTTAAGTT GAATTCTCTA CAGCTACAAG CTTTGTTGCA GAATTATCAC
    TGTGCTCCAG ATGAACCGCT TATACCAACA GAACTGATAG AGAATGTAGT AACTGTTGCA
    GAAAATACGG CTGATGAACT TGCTCGCAGT GATGGCAGAG AAGTCCAATT GGAAGAGGAT
    CCTGACTTAC AACTCCCTTT TCTTTTACCA GAAGATGGCT ATTCCTGTGA TGTTGTCAGA
    AATGTTCCAA GTGGTTTACA AGAATTTTTG GATCCCCTCT GTCAGAGAGG TTTTTGTAGG
    TTAATACCTC ATACACGGTC ACCAGGCACA TGGACAATAT ACTTTGAAGG CGCAGACTAT
    GAAAGCCACC TGTCACATGA GAATGCTGAG CTAGCTCAGC CTTTGAGGAA AGAACCTGAG
    ATTATAACTG TAACACTAAA AAAACAAAAT GGAATGGGCC TCAGCATTGT TGCTGCCAAG
    GGTGCTGGTC AAGACAAACT TGGAATATAT GTCAAGTCTG TTGTGAAAGG AGGTGCTGCA
    GATGTGGATG GTCGTCTGGC AGCAGGGGAT CAGCTGCTCA GTGTGGATGG TCGAAGCTTG
    GTGGGGCTGT CCCAGGAAAG GGCAGCAGAA CTTATGACGA GGACAGGTTC TGTTGTAACA
    CTGGAAGTAG CAAAACAAGG AGCAATATAC CACGGTCTGG CAACCCTGCT AAATCAGCCA
    TCTCCAATGA TGCAAAGAGT TTCTGACCGC CGTGGCTCAG GTAAACCCCG ACCAAAGAGT
    GAAGGTTTTG AGCTGTATAA CAACTCCACG CAAAATGGGT CACCTGAGAG CCCCCAGCTG
    CCATGGACGG AGTACAGTGA ACCAAAGAAG CTGCCAGGAG ATGACAGATT AATGAAGAAC
    AGAGCCGATC ATCGCTCCAG CCCCAACGTA GCAAATCAGC CTCCAAGCCC TGGGGGGAAG
    AATGCTTATG CATCTGGAAC TACCACCAAA ATAACCTCAG TCTCGACTGG AAATCTTTGT
    ACAGAGGAGC AAACTCTGCC TCCACGACCT GAAGCTTATC CTATCCCAAC ACAGACCTAT
    ACCAGAGAAT ATTTCACATT CCCAGCTTCA AAATCCCAGG ATCGAATGGG TCCAGCTCAG
    AGTCAGTGGC CAAATTATGA AGAGAAGCCA CAGATCCAGG CAGAAAGTAA TCACTCAATC
    AATACTACAA TGCAGCGCGT GGCTCGATCC CAGGAAGAAC TCCGAGATAA AGTTTTCCAG
    CCAGAGAGGA ATCGTTTGGA AGTTGTTATC CGGAAGGACA TAGAATACAT TGATCGGAAG
    TCAGACAGCG ATCAGTGGAT GAATTCATCT GTGGATTCTA GCACATCTAG CCAAGAGCAC
    CTGAACCATA CATCCAAACC TGTTTCACCT GGTTCTTGCT TAACTAAAAG TGGACCTGGC
    CGTTGGAAAA CTCCAGCTAC GAGCCAGCCA ACTCCAGTGG CCGTTTCCCA GCCCATCAGA
    ACAGACCTTC CTCCTCCGCC GCCACCTCCT CCGGTGCATT ATGCAGCTGA ATTTGATGAA
    CCACAAATGG ATTTGCCTCT GCCTCCCCCT CCTCCACCGA ACCAAGTAGG AGCACAAGCA
    TCTTCTCAGG TGGCTGCTGC TGAGCGTAAA AAACGAGAAG AGCATCAGAG GTGGTATGAA
    AAGGAAAAGG CACGTTTGGA AGAAGAACGA GAAAGGAAAC GCCGTGAGCA AGAGAGAAAA
    CTGGGCCAAA TGCGTTCGCA GCCACTAATT CCTCCCCAGC CTGTGGTTCC TCCTGTTTCC
    CTTCAGCAAG TGAAATCGGA AAAAACTTCA ACTTTCCAGA GGTCTCAAGA AACAGTCATT
    CGAGAGTTGC AGCCGCAACA GCAGCCTCGG ACTATTGAGC GGAGAGATCT GCAGTACATT
    ACTATCAGCA AGGAAGAATT GTCCTCTAGT GACAGCCTCT CTCCAGATCC CTGGAAACGA
    GATGCCAAGG AGAAACTGGA GAAGCAGCAG CAGATGCACA TTGTGGACAT GCTGAGCAAA
    GAGATTCAGG ATCTTCAGAA CAAGCCTGAT CGTACAGCTG AAGAGAACGA CCGCCTGCGC
    AAACTCATGC TAGAGTGGCA GTTCCAAAAG CGACTTCAGG AATCAAAACA AAAGGATGAA
    GATGATGATG AAGAAGAGGA CGATGATGTG GATACTATGC TTATCATGCA GCGGCTGGAG
    GCAGAAAGAA GAGCAAGGCA GACTGCTGTG CCAGCAATCT CTGTTCTAGA CTTGTTACAG
    GATGAAGAAC GCAGACGACA GCAACAGTTA GAAGAAATGC GCAAGCGAGA AGCAGAAGAC
    AGGGCCAGGC AGGAAGAGGA GCGCCGACAG CAAGAGGAGG AGCGAACCAG GAGAGAGGCA
    GAAGAAAAGA GGCGACAGGA AGAGGAGTAT TACAATCGCT TAGAGGCTGA AAGGCGCAGG
    CAGCACGATG AAGCAGAACG AAGATTGCTG GAACCAGACG AGCCTGGTCT GTACAGACCT
    CCACTTCCAC GGGAATATGA ACTCCCATCC CCAACCCCTT CATCTAACGC TCCTCCTCCC
    CCACCTCAGC GAAACACCTC TTACCTCAAA ACACAGGTCC TCTCCCCTGA CACGATTTAT
    ACTGCCAAGT TTGTTGCATA CAATGATGAT GAGGAGGAGG AGGATTCCAA TCTAGCAGGT
    CAGGATAAGT ACTCCAGCAC AAGAAAATCT TATGGTGACT TTCCTCCTGC CACCCTTAAA
    CCACAGCAGC CCTCCCGCCA GCCTCGCCCA GTTTCAGATG GCCTCTTTCT TTCCAACTCA
    TTCCAGTCAT CTCCAGTCAA TGCCAACAGT ACTGCTGCCA AAAAAAGCCA GCCCCCGCCC
    CCACCAAACA AACCGAGTTT CATCCATGCC AGCAGCTCTA AAGGAATAAA TTCATACACT
    GGATCTTCGG GAGCAACGGT TGGGGCCCAT GAAGTCTATC GGGATCCAAG AGACAAACGA
    TCTAAAAGTC AAGATACAGA TTTACCTGGA GCACCAGAAA ATCTGACGTT CAGGGAACGC
    CAGCGACTTT TTTCACAGGG TCAAGATGTA TCCAATAAAG TAAAAGCCTC TAGGAAACTA
    ATGGAACTGG AAAATGAGTT GAACACGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33802
    Clone ID Related Accession (Same CDS sequence) XM_015284352.1 , XM_015284352.2
    Accession Version XM_015284352.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5712bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGATGGTTC TGACTCCAGA
    GATAAACCAA AGCTTTATCG TCTACAATTA AGTGTCACTG AAGTTGGAAC TGAAAAATTT
    GATGACAATT CCATTCAGTT ATTTGGTCCA GGAATTCAGC CTCATCATTG TGATCTTACT
    AATATGGATG GAGTTGTTAC TGTAACCCCA AGAAACATTG ATGCGGAAAC GTATGTAGAA
    GGTCAGCGTA TTTCAGAAAC CACTATGCTG CAAAGTGGAA TGAAGGTTCA GTTTGGGTCA
    TCTCATGTGT TTAAGTTTGT GGATCCCACT CAGGACCATA TTATTACTAA AAGACCTATT
    GATGCAAGCC TTATGGTCAA AGGTCCAAGG CATAAAGCTG GAGCTGTTCA GGAAACCACG
    TTTGATCTGG AGGGAGATGT TCACAGTGGA ACAGCGTTAC CAGCGAGCAA GAGCACCAGC
    AGGCTTGATG GTGACAGAAC ATCATCAGCA TCCAGCACAG CTGAACGAGG AATGGTGAAG
    CCCATGATTA GAATAGAACA GCAGCAAGAT TATCGCAGAC AGGAAAGCAG ACCTCAGGAT
    ACCCATGGAC CAGAACTGAT CCTACCTGCC AGTATTGAAT TCAGGGAAAG CTCTGAAGAT
    GCCTTCTTAT CTGCCATTAT AAATTATACA AATAGCTCGA CAGTTCATTT TAAACTGTCA
    CCCACGTATG TTTTGTATAT GACATGTCGG TATGTATTGT CAAGCCAGTA CAGACCTGAC
    ATCACTCCTA CTGAGCGTAC TCACAAAGTT ATTGCAATAG TCAACAAGAT GGTGAATATG
    ATGGAAGGAG TCATACAGAA ACAGAAGAAT ATTGCTGGGG CACTTGCCTT TTGGATGGCA
    AATGCGTCTG AATTGCTGAA TTTTATAAAG CAAGACCGTG ATCTGAGCCG AATCACTGTG
    GATGCTCAAG ATGTTTTGGC GCACTTGGTT CAAATGGCAT TCAAGTACCT AGTTCATTGT
    CTGCAGTCAG AGCTTAATAA CTACATGCCA GCATTTCTTG ATGATCCAGA GGAAAATAAT
    CCACAGAGGC CTAAAATAGA TGATGTGCTG CACACATTGA CTGGAGCTAT GTCCTTGTTG
    CGACGATGTA GAGTGAATGC TGCACTGACT ATACAGCTCT TCTCCCAGTT GTTTCATTTT
    ATCAACATGT GGCTTTTTAA CAGACTAGTT ACGGCTCCAG ATTCAGGGTT ATGTTCCCAC
    TACTGGGGAG CTATTGTTCG TCAGCAGTTG GGTCATATTG AGGCCTGGGC AGAAAAACAG
    GGTTTAGAAT TGGCTGCTGA TTGCCACTTA AGCAGAATAG TGCAGGCAAC CACATTGCTC
    ACCATGGACA AGTACTCACA TGCTGATGTT CCAAATATTA ACAGTACTTG CTTTAAGTTG
    AATTCTCTAC AGCTACAAGC TTTGTTGCAG AATTATCACT GTGCTCCAGA TGAACCGCTT
    ATACCAACAG AACTGATAGA GAATGTAGTA ACTGTTGCAG AAAATACGGC TGATGAACTT
    GCTCGCAGTG ATGGCAGAGA AGTCCAATTG GAAGAGGATC CTGACTTACA ACTCCCTTTT
    CTTTTACCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ATGTTCCAAG TGGTTTACAA
    GAATTTTTGG ATCCCCTCTG TCAGAGAGGT TTTTGTAGGT TAATACCTCA TACACGGTCA
    CCAGGCACAT GGACAATATA CTTTGAAGGC GCAGACTATG AAAGCCACCT GTCACATGAG
    AATGCTGAGC TAGCTCAGCC TTTGAGGAAA GAACCTGAGA TTATAACTGT AACACTAAAA
    AAACAAAATG GAATGGGCCT CAGCATTGTT GCTGCCAAGG GTGCTGGTCA AGACAAACTT
    GGAATATATG TCAAGTCTGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG TCGTCTGGCA
    GCAGGGGATC AGCTGCTCAG TGTGGATGGT CGAAGCTTGG TGGGGCTGTC CCAGGAAAGG
    GCAGCAGAAC TTATGACGAG GACAGGTTCT GTTGTAACAC TGGAAGTAGC AAAACAAGGA
    GCAATATACC ACGGTCTGGC AACCCTGCTA AATCAGCCAT CTCCAATGAT GCAAAGAGTT
    TCTGACCGCC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGTTTTGA GCTGTATAAC
    AACTCCACGC AAAATGGGTC ACCTGAGAGC CCCCAGCTGC CATGGACGGA GTACAGTGAA
    CCAAAGAAGC TGCCAGGAGA TGACAGATTA ATGAAGAACA GAGCCGATCA TCGCTCCAGC
    CCCAACGTAG CAAATCAGCC TCCAAGCCCT GGGGGGAAGA ATGCTTATGC ATCTGGAACT
    ACCACCAAAA TAACCTCAGT CTCGACTGGA AATCTTTGTA CAGAGGAGCA AACTCTGCCT
    CCACGACCTG AAGCTTATCC TATCCCAACA CAGACCTATA CCAGAGAATA TTTCACATTC
    CCAGCTTCAA AATCCCAGGA TCGAATGGGT CCAGCTCAGA GTCAGTGGCC AAATTATGAA
    GAGAAGCCAC AGATCCAGGC AGAAAGTAAT CACTCAATCA ATACTACAAT GCAGCGCGTG
    GCTCGATCCC AGGAAGAACT CCGAGATAAA GTTTTCCAGC CAGAGAGGAA TCGTTTGGAA
    GTTGTTATCC GGAAGGACAT AGAATACATT GATCGGAAGT CAGACAGCGA TCAGTGGATG
    AATTCATCTG TGGATTCTAG CACATCTAGC CAAGAGCACC TGAACCATAC ATCCAAACCT
    GTTTCACCTG GTTCTTGCTT AACTAAAAGT GGACCTGGCC GTTGGAAAAC TCCAGCTACG
    AGCCAGCCAA CTCCAGTGGC CGTTTCCCAG CCCATCAGAA CAGACCTTCC TCCTCCGCCG
    CCACCTCCTC CGGTGCATTA TGCAGCTGAA TTTGATGAAC CACAAATGGA TTTGCCTCTG
    CCTCCCCCTC CTCCACCGAA CCAAGTAGGA GCACAAGCAT CTTCTCAGGT GGCTGCTGCT
    GAGCGTAAAA AACGAGAAGA GCATCAGAGG TGGTATGAAA AGGAAAAGGC ACGTTTGGAA
    GAAGAACGAG AAAGGAAACG CCGTGAGCAA GAGAGAAAAC TGGGCCAAAT GCGTTCGCAG
    CCACTAATTC CTCCCCAGCC TGTGGTTCCT CCTGTTTCCC TTCAGCAAGT GAAATCGGAA
    AAAACTTCAA CTTTCCAGAG GTCTCAAGAA ACAGTCATTC GAGAGTTGCA GCCGCAACAG
    CAGCCTCGGA CTATTGAGCG GAGAGATCTG CAGTACATTA CTATCAGCAA GGAAGAATTG
    TCCTCTAGTG ACAGCCTCTC TCCAGATCCC TGGAAACGAG ATGCCAAGGA GAAACTGGAG
    AAGCAGCAGC AGATGCACAT TGTGGACATG CTGAGCAAAG AGATTCAGGA TCTTCAGAAC
    AAGCCTGATC GTACAGCTGA AGAGAACGAC CGCCTGCGCA AACTCATGCT AGAGTGGCAG
    TTCCAAAAGC GACTTCAGGA ATCAAAACAA AAGGATGAAG ATGATGATGA AGAAGAGGAC
    GATGATGTGG ATACTATGCT TATCATGCAG CGGCTGGAGG CAGAAAGAAG AGCAAGGCAG
    ACTGCTGTGC CAGCAATCTC TGTTCTAGAC TTGTTACAGG ATGAAGAACG CAGACGACAG
    CAACAGTTAG AAGAAATGCG CAAGCGAGAA GCAGAAGACA GGGCCAGGCA GGAAGAGGAG
    CGCCGACAGC AAGAGGAGGA GCGAACCAGG AGAGAGGCAG AAGAAAAGAG GCGACAGGAA
    GAGGAGTATT ACAATCGCTT AGAGGCTGAA AGGCGCAGGC AGCACGATGA AGCAGAACGA
    AGATTGCTGG AACCAGACGA GCCTGGTCTG TACAGACCTC CACTTCCACG GGAATATGAA
    CTCCCATCCC CAACCCCTTC ATCTAACGCT CCTCCTCCCC CACCTCAGCG AAACACCTCT
    TACCTCAAAA CACAGGTCCT CTCCCCTGAC ACGATTTATA CTGCCAAGTT TGTTGCATAC
    AATGATGATG AGGAGGAGGA GGATTCCAAT CTAGCAGGTC AGGATAAGTA CTCCAGCACA
    AGAAAATCTT ATGGTGACTT TCCTCCTGCC ACCCTTAAAC CACAGCAGCC CTCCCGCCAG
    CCTCGCCCAG TTTCAGATGG CCTCTTTCTT TCCAACTCAT TCCAGTCATC TCCAGTCAAT
    GCCAACAGTA CTGCTGCCAA AAAAAGCCAG CCCCCGCCCC CACCAAACAA ACCGAGTTTC
    ATCCATGCCA GCAGCTCTAA AGGAATAAAT TCATACACTG GATCTTCGGG AGCAACGGTT
    GGGGCCCATG AAGTCTATCG GGATCCAAGA GACAAACGAT CTAAAAGTCA AGATACAGAT
    TTACCTGGAG CACCAGAAAA TCTGACGTTC AGGGAACGCC AGCGACTTTT TTCACAGGGT
    CAAGATGTAT CCAATAAAGT AAAAGCCTCT AGGAAACTAA TGGAACTGGA AAATGAGTTG
    AACACGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139838.1
    CDS33..5744
    Translation

    Target ORF information:

    RefSeq Version XM_015284352.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284352.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGATGGTTC TGACTCCAGA
    GATAAACCAA AGCTTTATCG TCTACAATTA AGTGTCACTG AAGTTGGAAC TGAAAAATTT
    GATGACAATT CCATTCAGTT ATTTGGTCCA GGAATTCAGC CTCATCATTG TGATCTTACT
    AATATGGATG GAGTTGTTAC TGTAACCCCA AGAAACATTG ATGCGGAAAC GTATGTAGAA
    GGTCAGCGTA TTTCAGAAAC CACTATGCTG CAAAGTGGAA TGAAGGTTCA GTTTGGGTCA
    TCTCATGTGT TTAAGTTTGT GGATCCCACT CAGGACCATA TTATTACTAA AAGACCTATT
    GATGCAAGCC TTATGGTCAA AGGTCCAAGG CATAAAGCTG GAGCTGTTCA GGAAACCACG
    TTTGATCTGG AGGGAGATGT TCACAGTGGA ACAGCGTTAC CAGCGAGCAA GAGCACCAGC
    AGGCTTGATG GTGACAGAAC ATCATCAGCA TCCAGCACAG CTGAACGAGG AATGGTGAAG
    CCCATGATTA GAATAGAACA GCAGCAAGAT TATCGCAGAC AGGAAAGCAG ACCTCAGGAT
    ACCCATGGAC CAGAACTGAT CCTACCTGCC AGTATTGAAT TCAGGGAAAG CTCTGAAGAT
    GCCTTCTTAT CTGCCATTAT AAATTATACA AATAGCTCGA CAGTTCATTT TAAACTGTCA
    CCCACGTATG TTTTGTATAT GACATGTCGG TATGTATTGT CAAGCCAGTA CAGACCTGAC
    ATCACTCCTA CTGAGCGTAC TCACAAAGTT ATTGCAATAG TCAACAAGAT GGTGAATATG
    ATGGAAGGAG TCATACAGAA ACAGAAGAAT ATTGCTGGGG CACTTGCCTT TTGGATGGCA
    AATGCGTCTG AATTGCTGAA TTTTATAAAG CAAGACCGTG ATCTGAGCCG AATCACTGTG
    GATGCTCAAG ATGTTTTGGC GCACTTGGTT CAAATGGCAT TCAAGTACCT AGTTCATTGT
    CTGCAGTCAG AGCTTAATAA CTACATGCCA GCATTTCTTG ATGATCCAGA GGAAAATAAT
    CCACAGAGGC CTAAAATAGA TGATGTGCTG CACACATTGA CTGGAGCTAT GTCCTTGTTG
    CGACGATGTA GAGTGAATGC TGCACTGACT ATACAGCTCT TCTCCCAGTT GTTTCATTTT
    ATCAACATGT GGCTTTTTAA CAGACTAGTT ACGGCTCCAG ATTCAGGGTT ATGTTCCCAC
    TACTGGGGAG CTATTGTTCG TCAGCAGTTG GGTCATATTG AGGCCTGGGC AGAAAAACAG
    GGTTTAGAAT TGGCTGCTGA TTGCCACTTA AGCAGAATAG TGCAGGCAAC CACATTGCTC
    ACCATGGACA AGTACTCACA TGCTGATGTT CCAAATATTA ACAGTACTTG CTTTAAGTTG
    AATTCTCTAC AGCTACAAGC TTTGTTGCAG AATTATCACT GTGCTCCAGA TGAACCGCTT
    ATACCAACAG AACTGATAGA GAATGTAGTA ACTGTTGCAG AAAATACGGC TGATGAACTT
    GCTCGCAGTG ATGGCAGAGA AGTCCAATTG GAAGAGGATC CTGACTTACA ACTCCCTTTT
    CTTTTACCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ATGTTCCAAG TGGTTTACAA
    GAATTTTTGG ATCCCCTCTG TCAGAGAGGT TTTTGTAGGT TAATACCTCA TACACGGTCA
    CCAGGCACAT GGACAATATA CTTTGAAGGC GCAGACTATG AAAGCCACCT GTCACATGAG
    AATGCTGAGC TAGCTCAGCC TTTGAGGAAA GAACCTGAGA TTATAACTGT AACACTAAAA
    AAACAAAATG GAATGGGCCT CAGCATTGTT GCTGCCAAGG GTGCTGGTCA AGACAAACTT
    GGAATATATG TCAAGTCTGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG TCGTCTGGCA
    GCAGGGGATC AGCTGCTCAG TGTGGATGGT CGAAGCTTGG TGGGGCTGTC CCAGGAAAGG
    GCAGCAGAAC TTATGACGAG GACAGGTTCT GTTGTAACAC TGGAAGTAGC AAAACAAGGA
    GCAATATACC ACGGTCTGGC AACCCTGCTA AATCAGCCAT CTCCAATGAT GCAAAGAGTT
    TCTGACCGCC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGTTTTGA GCTGTATAAC
    AACTCCACGC AAAATGGGTC ACCTGAGAGC CCCCAGCTGC CATGGACGGA GTACAGTGAA
    CCAAAGAAGC TGCCAGGAGA TGACAGATTA ATGAAGAACA GAGCCGATCA TCGCTCCAGC
    CCCAACGTAG CAAATCAGCC TCCAAGCCCT GGGGGGAAGA ATGCTTATGC ATCTGGAACT
    ACCACCAAAA TAACCTCAGT CTCGACTGGA AATCTTTGTA CAGAGGAGCA AACTCTGCCT
    CCACGACCTG AAGCTTATCC TATCCCAACA CAGACCTATA CCAGAGAATA TTTCACATTC
    CCAGCTTCAA AATCCCAGGA TCGAATGGGT CCAGCTCAGA GTCAGTGGCC AAATTATGAA
    GAGAAGCCAC AGATCCAGGC AGAAAGTAAT CACTCAATCA ATACTACAAT GCAGCGCGTG
    GCTCGATCCC AGGAAGAACT CCGAGATAAA GTTTTCCAGC CAGAGAGGAA TCGTTTGGAA
    GTTGTTATCC GGAAGGACAT AGAATACATT GATCGGAAGT CAGACAGCGA TCAGTGGATG
    AATTCATCTG TGGATTCTAG CACATCTAGC CAAGAGCACC TGAACCATAC ATCCAAACCT
    GTTTCACCTG GTTCTTGCTT AACTAAAAGT GGACCTGGCC GTTGGAAAAC TCCAGCTACG
    AGCCAGCCAA CTCCAGTGGC CGTTTCCCAG CCCATCAGAA CAGACCTTCC TCCTCCGCCG
    CCACCTCCTC CGGTGCATTA TGCAGCTGAA TTTGATGAAC CACAAATGGA TTTGCCTCTG
    CCTCCCCCTC CTCCACCGAA CCAAGTAGGA GCACAAGCAT CTTCTCAGGT GGCTGCTGCT
    GAGCGTAAAA AACGAGAAGA GCATCAGAGG TGGTATGAAA AGGAAAAGGC ACGTTTGGAA
    GAAGAACGAG AAAGGAAACG CCGTGAGCAA GAGAGAAAAC TGGGCCAAAT GCGTTCGCAG
    CCACTAATTC CTCCCCAGCC TGTGGTTCCT CCTGTTTCCC TTCAGCAAGT GAAATCGGAA
    AAAACTTCAA CTTTCCAGAG GTCTCAAGAA ACAGTCATTC GAGAGTTGCA GCCGCAACAG
    CAGCCTCGGA CTATTGAGCG GAGAGATCTG CAGTACATTA CTATCAGCAA GGAAGAATTG
    TCCTCTAGTG ACAGCCTCTC TCCAGATCCC TGGAAACGAG ATGCCAAGGA GAAACTGGAG
    AAGCAGCAGC AGATGCACAT TGTGGACATG CTGAGCAAAG AGATTCAGGA TCTTCAGAAC
    AAGCCTGATC GTACAGCTGA AGAGAACGAC CGCCTGCGCA AACTCATGCT AGAGTGGCAG
    TTCCAAAAGC GACTTCAGGA ATCAAAACAA AAGGATGAAG ATGATGATGA AGAAGAGGAC
    GATGATGTGG ATACTATGCT TATCATGCAG CGGCTGGAGG CAGAAAGAAG AGCAAGGCAG
    ACTGCTGTGC CAGCAATCTC TGTTCTAGAC TTGTTACAGG ATGAAGAACG CAGACGACAG
    CAACAGTTAG AAGAAATGCG CAAGCGAGAA GCAGAAGACA GGGCCAGGCA GGAAGAGGAG
    CGCCGACAGC AAGAGGAGGA GCGAACCAGG AGAGAGGCAG AAGAAAAGAG GCGACAGGAA
    GAGGAGTATT ACAATCGCTT AGAGGCTGAA AGGCGCAGGC AGCACGATGA AGCAGAACGA
    AGATTGCTGG AACCAGACGA GCCTGGTCTG TACAGACCTC CACTTCCACG GGAATATGAA
    CTCCCATCCC CAACCCCTTC ATCTAACGCT CCTCCTCCCC CACCTCAGCG AAACACCTCT
    TACCTCAAAA CACAGGTCCT CTCCCCTGAC ACGATTTATA CTGCCAAGTT TGTTGCATAC
    AATGATGATG AGGAGGAGGA GGATTCCAAT CTAGCAGGTC AGGATAAGTA CTCCAGCACA
    AGAAAATCTT ATGGTGACTT TCCTCCTGCC ACCCTTAAAC CACAGCAGCC CTCCCGCCAG
    CCTCGCCCAG TTTCAGATGG CCTCTTTCTT TCCAACTCAT TCCAGTCATC TCCAGTCAAT
    GCCAACAGTA CTGCTGCCAA AAAAAGCCAG CCCCCGCCCC CACCAAACAA ACCGAGTTTC
    ATCCATGCCA GCAGCTCTAA AGGAATAAAT TCATACACTG GATCTTCGGG AGCAACGGTT
    GGGGCCCATG AAGTCTATCG GGATCCAAGA GACAAACGAT CTAAAAGTCA AGATACAGAT
    TTACCTGGAG CACCAGAAAA TCTGACGTTC AGGGAACGCC AGCGACTTTT TTCACAGGGT
    CAAGATGTAT CCAATAAAGT AAAAGCCTCT AGGAAACTAA TGGAACTGGA AAATGAGTTG
    AACACGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33802
    Clone ID Related Accession (Same CDS sequence) XM_015284352.1 , XM_015284352.2
    Accession Version XM_015284352.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5712bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284352.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGATGGTTC TGACTCCAGA
    GATAAACCAA AGCTTTATCG TCTACAATTA AGTGTCACTG AAGTTGGAAC TGAAAAATTT
    GATGACAATT CCATTCAGTT ATTTGGTCCA GGAATTCAGC CTCATCATTG TGATCTTACT
    AATATGGATG GAGTTGTTAC TGTAACCCCA AGAAACATTG ATGCGGAAAC GTATGTAGAA
    GGTCAGCGTA TTTCAGAAAC CACTATGCTG CAAAGTGGAA TGAAGGTTCA GTTTGGGTCA
    TCTCATGTGT TTAAGTTTGT GGATCCCACT CAGGACCATA TTATTACTAA AAGACCTATT
    GATGCAAGCC TTATGGTCAA AGGTCCAAGG CATAAAGCTG GAGCTGTTCA GGAAACCACG
    TTTGATCTGG AGGGAGATGT TCACAGTGGA ACAGCGTTAC CAGCGAGCAA GAGCACCAGC
    AGGCTTGATG GTGACAGAAC ATCATCAGCA TCCAGCACAG CTGAACGAGG AATGGTGAAG
    CCCATGATTA GAATAGAACA GCAGCAAGAT TATCGCAGAC AGGAAAGCAG ACCTCAGGAT
    ACCCATGGAC CAGAACTGAT CCTACCTGCC AGTATTGAAT TCAGGGAAAG CTCTGAAGAT
    GCCTTCTTAT CTGCCATTAT AAATTATACA AATAGCTCGA CAGTTCATTT TAAACTGTCA
    CCCACGTATG TTTTGTATAT GACATGTCGG TATGTATTGT CAAGCCAGTA CAGACCTGAC
    ATCACTCCTA CTGAGCGTAC TCACAAAGTT ATTGCAATAG TCAACAAGAT GGTGAATATG
    ATGGAAGGAG TCATACAGAA ACAGAAGAAT ATTGCTGGGG CACTTGCCTT TTGGATGGCA
    AATGCGTCTG AATTGCTGAA TTTTATAAAG CAAGACCGTG ATCTGAGCCG AATCACTGTG
    GATGCTCAAG ATGTTTTGGC GCACTTGGTT CAAATGGCAT TCAAGTACCT AGTTCATTGT
    CTGCAGTCAG AGCTTAATAA CTACATGCCA GCATTTCTTG ATGATCCAGA GGAAAATAAT
    CCACAGAGGC CTAAAATAGA TGATGTGCTG CACACATTGA CTGGAGCTAT GTCCTTGTTG
    CGACGATGTA GAGTGAATGC TGCACTGACT ATACAGCTCT TCTCCCAGTT GTTTCATTTT
    ATCAACATGT GGCTTTTTAA CAGACTAGTT ACGGCTCCAG ATTCAGGGTT ATGTTCCCAC
    TACTGGGGAG CTATTGTTCG TCAGCAGTTG GGTCATATTG AGGCCTGGGC AGAAAAACAG
    GGTTTAGAAT TGGCTGCTGA TTGCCACTTA AGCAGAATAG TGCAGGCAAC CACATTGCTC
    ACCATGGACA AGTACTCACA TGCTGATGTT CCAAATATTA ACAGTACTTG CTTTAAGTTG
    AATTCTCTAC AGCTACAAGC TTTGTTGCAG AATTATCACT GTGCTCCAGA TGAACCGCTT
    ATACCAACAG AACTGATAGA GAATGTAGTA ACTGTTGCAG AAAATACGGC TGATGAACTT
    GCTCGCAGTG ATGGCAGAGA AGTCCAATTG GAAGAGGATC CTGACTTACA ACTCCCTTTT
    CTTTTACCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ATGTTCCAAG TGGTTTACAA
    GAATTTTTGG ATCCCCTCTG TCAGAGAGGT TTTTGTAGGT TAATACCTCA TACACGGTCA
    CCAGGCACAT GGACAATATA CTTTGAAGGC GCAGACTATG AAAGCCACCT GTCACATGAG
    AATGCTGAGC TAGCTCAGCC TTTGAGGAAA GAACCTGAGA TTATAACTGT AACACTAAAA
    AAACAAAATG GAATGGGCCT CAGCATTGTT GCTGCCAAGG GTGCTGGTCA AGACAAACTT
    GGAATATATG TCAAGTCTGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG TCGTCTGGCA
    GCAGGGGATC AGCTGCTCAG TGTGGATGGT CGAAGCTTGG TGGGGCTGTC CCAGGAAAGG
    GCAGCAGAAC TTATGACGAG GACAGGTTCT GTTGTAACAC TGGAAGTAGC AAAACAAGGA
    GCAATATACC ACGGTCTGGC AACCCTGCTA AATCAGCCAT CTCCAATGAT GCAAAGAGTT
    TCTGACCGCC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGTTTTGA GCTGTATAAC
    AACTCCACGC AAAATGGGTC ACCTGAGAGC CCCCAGCTGC CATGGACGGA GTACAGTGAA
    CCAAAGAAGC TGCCAGGAGA TGACAGATTA ATGAAGAACA GAGCCGATCA TCGCTCCAGC
    CCCAACGTAG CAAATCAGCC TCCAAGCCCT GGGGGGAAGA ATGCTTATGC ATCTGGAACT
    ACCACCAAAA TAACCTCAGT CTCGACTGGA AATCTTTGTA CAGAGGAGCA AACTCTGCCT
    CCACGACCTG AAGCTTATCC TATCCCAACA CAGACCTATA CCAGAGAATA TTTCACATTC
    CCAGCTTCAA AATCCCAGGA TCGAATGGGT CCAGCTCAGA GTCAGTGGCC AAATTATGAA
    GAGAAGCCAC AGATCCAGGC AGAAAGTAAT CACTCAATCA ATACTACAAT GCAGCGCGTG
    GCTCGATCCC AGGAAGAACT CCGAGATAAA GTTTTCCAGC CAGAGAGGAA TCGTTTGGAA
    GTTGTTATCC GGAAGGACAT AGAATACATT GATCGGAAGT CAGACAGCGA TCAGTGGATG
    AATTCATCTG TGGATTCTAG CACATCTAGC CAAGAGCACC TGAACCATAC ATCCAAACCT
    GTTTCACCTG GTTCTTGCTT AACTAAAAGT GGACCTGGCC GTTGGAAAAC TCCAGCTACG
    AGCCAGCCAA CTCCAGTGGC CGTTTCCCAG CCCATCAGAA CAGACCTTCC TCCTCCGCCG
    CCACCTCCTC CGGTGCATTA TGCAGCTGAA TTTGATGAAC CACAAATGGA TTTGCCTCTG
    CCTCCCCCTC CTCCACCGAA CCAAGTAGGA GCACAAGCAT CTTCTCAGGT GGCTGCTGCT
    GAGCGTAAAA AACGAGAAGA GCATCAGAGG TGGTATGAAA AGGAAAAGGC ACGTTTGGAA
    GAAGAACGAG AAAGGAAACG CCGTGAGCAA GAGAGAAAAC TGGGCCAAAT GCGTTCGCAG
    CCACTAATTC CTCCCCAGCC TGTGGTTCCT CCTGTTTCCC TTCAGCAAGT GAAATCGGAA
    AAAACTTCAA CTTTCCAGAG GTCTCAAGAA ACAGTCATTC GAGAGTTGCA GCCGCAACAG
    CAGCCTCGGA CTATTGAGCG GAGAGATCTG CAGTACATTA CTATCAGCAA GGAAGAATTG
    TCCTCTAGTG ACAGCCTCTC TCCAGATCCC TGGAAACGAG ATGCCAAGGA GAAACTGGAG
    AAGCAGCAGC AGATGCACAT TGTGGACATG CTGAGCAAAG AGATTCAGGA TCTTCAGAAC
    AAGCCTGATC GTACAGCTGA AGAGAACGAC CGCCTGCGCA AACTCATGCT AGAGTGGCAG
    TTCCAAAAGC GACTTCAGGA ATCAAAACAA AAGGATGAAG ATGATGATGA AGAAGAGGAC
    GATGATGTGG ATACTATGCT TATCATGCAG CGGCTGGAGG CAGAAAGAAG AGCAAGGCAG
    ACTGCTGTGC CAGCAATCTC TGTTCTAGAC TTGTTACAGG ATGAAGAACG CAGACGACAG
    CAACAGTTAG AAGAAATGCG CAAGCGAGAA GCAGAAGACA GGGCCAGGCA GGAAGAGGAG
    CGCCGACAGC AAGAGGAGGA GCGAACCAGG AGAGAGGCAG AAGAAAAGAG GCGACAGGAA
    GAGGAGTATT ACAATCGCTT AGAGGCTGAA AGGCGCAGGC AGCACGATGA AGCAGAACGA
    AGATTGCTGG AACCAGACGA GCCTGGTCTG TACAGACCTC CACTTCCACG GGAATATGAA
    CTCCCATCCC CAACCCCTTC ATCTAACGCT CCTCCTCCCC CACCTCAGCG AAACACCTCT
    TACCTCAAAA CACAGGTCCT CTCCCCTGAC ACGATTTATA CTGCCAAGTT TGTTGCATAC
    AATGATGATG AGGAGGAGGA GGATTCCAAT CTAGCAGGTC AGGATAAGTA CTCCAGCACA
    AGAAAATCTT ATGGTGACTT TCCTCCTGCC ACCCTTAAAC CACAGCAGCC CTCCCGCCAG
    CCTCGCCCAG TTTCAGATGG CCTCTTTCTT TCCAACTCAT TCCAGTCATC TCCAGTCAAT
    GCCAACAGTA CTGCTGCCAA AAAAAGCCAG CCCCCGCCCC CACCAAACAA ACCGAGTTTC
    ATCCATGCCA GCAGCTCTAA AGGAATAAAT TCATACACTG GATCTTCGGG AGCAACGGTT
    GGGGCCCATG AAGTCTATCG GGATCCAAGA GACAAACGAT CTAAAAGTCA AGATACAGAT
    TTACCTGGAG CACCAGAAAA TCTGACGTTC AGGGAACGCC AGCGACTTTT TTCACAGGGT
    CAAGATGTAT CCAATAAAGT AAAAGCCTCT AGGAAACTAA TGGAACTGGA AAATGAGTTG
    AACACGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139838.1
    CDS2885..8596
    Translation

    Target ORF information:

    RefSeq Version XM_015284352.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284352.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGATGGTTC TGACTCCAGA
    GATAAACCAA AGCTTTATCG TCTACAATTA AGTGTCACTG AAGTTGGAAC TGAAAAATTT
    GATGACAATT CCATTCAGTT ATTTGGTCCA GGAATTCAGC CTCATCATTG TGATCTTACT
    AATATGGATG GAGTTGTTAC TGTAACCCCA AGAAACATTG ATGCGGAAAC GTATGTAGAA
    GGTCAGCGTA TTTCAGAAAC CACTATGCTG CAAAGTGGAA TGAAGGTTCA GTTTGGGTCA
    TCTCATGTGT TTAAGTTTGT GGATCCCACT CAGGACCATA TTATTACTAA AAGACCTATT
    GATGCAAGCC TTATGGTCAA AGGTCCAAGG CATAAAGCTG GAGCTGTTCA GGAAACCACG
    TTTGATCTGG AGGGAGATGT TCACAGTGGA ACAGCGTTAC CAGCGAGCAA GAGCACCAGC
    AGGCTTGATG GTGACAGAAC ATCATCAGCA TCCAGCACAG CTGAACGAGG AATGGTGAAG
    CCCATGATTA GAATAGAACA GCAGCAAGAT TATCGCAGAC AGGAAAGCAG ACCTCAGGAT
    ACCCATGGAC CAGAACTGAT CCTACCTGCC AGTATTGAAT TCAGGGAAAG CTCTGAAGAT
    GCCTTCTTAT CTGCCATTAT AAATTATACA AATAGCTCGA CAGTTCATTT TAAACTGTCA
    CCCACGTATG TTTTGTATAT GACATGTCGG TATGTATTGT CAAGCCAGTA CAGACCTGAC
    ATCACTCCTA CTGAGCGTAC TCACAAAGTT ATTGCAATAG TCAACAAGAT GGTGAATATG
    ATGGAAGGAG TCATACAGAA ACAGAAGAAT ATTGCTGGGG CACTTGCCTT TTGGATGGCA
    AATGCGTCTG AATTGCTGAA TTTTATAAAG CAAGACCGTG ATCTGAGCCG AATCACTGTG
    GATGCTCAAG ATGTTTTGGC GCACTTGGTT CAAATGGCAT TCAAGTACCT AGTTCATTGT
    CTGCAGTCAG AGCTTAATAA CTACATGCCA GCATTTCTTG ATGATCCAGA GGAAAATAAT
    CCACAGAGGC CTAAAATAGA TGATGTGCTG CACACATTGA CTGGAGCTAT GTCCTTGTTG
    CGACGATGTA GAGTGAATGC TGCACTGACT ATACAGCTCT TCTCCCAGTT GTTTCATTTT
    ATCAACATGT GGCTTTTTAA CAGACTAGTT ACGGCTCCAG ATTCAGGGTT ATGTTCCCAC
    TACTGGGGAG CTATTGTTCG TCAGCAGTTG GGTCATATTG AGGCCTGGGC AGAAAAACAG
    GGTTTAGAAT TGGCTGCTGA TTGCCACTTA AGCAGAATAG TGCAGGCAAC CACATTGCTC
    ACCATGGACA AGTACTCACA TGCTGATGTT CCAAATATTA ACAGTACTTG CTTTAAGTTG
    AATTCTCTAC AGCTACAAGC TTTGTTGCAG AATTATCACT GTGCTCCAGA TGAACCGCTT
    ATACCAACAG AACTGATAGA GAATGTAGTA ACTGTTGCAG AAAATACGGC TGATGAACTT
    GCTCGCAGTG ATGGCAGAGA AGTCCAATTG GAAGAGGATC CTGACTTACA ACTCCCTTTT
    CTTTTACCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ATGTTCCAAG TGGTTTACAA
    GAATTTTTGG ATCCCCTCTG TCAGAGAGGT TTTTGTAGGT TAATACCTCA TACACGGTCA
    CCAGGCACAT GGACAATATA CTTTGAAGGC GCAGACTATG AAAGCCACCT GTCACATGAG
    AATGCTGAGC TAGCTCAGCC TTTGAGGAAA GAACCTGAGA TTATAACTGT AACACTAAAA
    AAACAAAATG GAATGGGCCT CAGCATTGTT GCTGCCAAGG GTGCTGGTCA AGACAAACTT
    GGAATATATG TCAAGTCTGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG TCGTCTGGCA
    GCAGGGGATC AGCTGCTCAG TGTGGATGGT CGAAGCTTGG TGGGGCTGTC CCAGGAAAGG
    GCAGCAGAAC TTATGACGAG GACAGGTTCT GTTGTAACAC TGGAAGTAGC AAAACAAGGA
    GCAATATACC ACGGTCTGGC AACCCTGCTA AATCAGCCAT CTCCAATGAT GCAAAGAGTT
    TCTGACCGCC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGTTTTGA GCTGTATAAC
    AACTCCACGC AAAATGGGTC ACCTGAGAGC CCCCAGCTGC CATGGACGGA GTACAGTGAA
    CCAAAGAAGC TGCCAGGAGA TGACAGATTA ATGAAGAACA GAGCCGATCA TCGCTCCAGC
    CCCAACGTAG CAAATCAGCC TCCAAGCCCT GGGGGGAAGA ATGCTTATGC ATCTGGAACT
    ACCACCAAAA TAACCTCAGT CTCGACTGGA AATCTTTGTA CAGAGGAGCA AACTCTGCCT
    CCACGACCTG AAGCTTATCC TATCCCAACA CAGACCTATA CCAGAGAATA TTTCACATTC
    CCAGCTTCAA AATCCCAGGA TCGAATGGGT CCAGCTCAGA GTCAGTGGCC AAATTATGAA
    GAGAAGCCAC AGATCCAGGC AGAAAGTAAT CACTCAATCA ATACTACAAT GCAGCGCGTG
    GCTCGATCCC AGGAAGAACT CCGAGATAAA GTTTTCCAGC CAGAGAGGAA TCGTTTGGAA
    GTTGTTATCC GGAAGGACAT AGAATACATT GATCGGAAGT CAGACAGCGA TCAGTGGATG
    AATTCATCTG TGGATTCTAG CACATCTAGC CAAGAGCACC TGAACCATAC ATCCAAACCT
    GTTTCACCTG GTTCTTGCTT AACTAAAAGT GGACCTGGCC GTTGGAAAAC TCCAGCTACG
    AGCCAGCCAA CTCCAGTGGC CGTTTCCCAG CCCATCAGAA CAGACCTTCC TCCTCCGCCG
    CCACCTCCTC CGGTGCATTA TGCAGCTGAA TTTGATGAAC CACAAATGGA TTTGCCTCTG
    CCTCCCCCTC CTCCACCGAA CCAAGTAGGA GCACAAGCAT CTTCTCAGGT GGCTGCTGCT
    GAGCGTAAAA AACGAGAAGA GCATCAGAGG TGGTATGAAA AGGAAAAGGC ACGTTTGGAA
    GAAGAACGAG AAAGGAAACG CCGTGAGCAA GAGAGAAAAC TGGGCCAAAT GCGTTCGCAG
    CCACTAATTC CTCCCCAGCC TGTGGTTCCT CCTGTTTCCC TTCAGCAAGT GAAATCGGAA
    AAAACTTCAA CTTTCCAGAG GTCTCAAGAA ACAGTCATTC GAGAGTTGCA GCCGCAACAG
    CAGCCTCGGA CTATTGAGCG GAGAGATCTG CAGTACATTA CTATCAGCAA GGAAGAATTG
    TCCTCTAGTG ACAGCCTCTC TCCAGATCCC TGGAAACGAG ATGCCAAGGA GAAACTGGAG
    AAGCAGCAGC AGATGCACAT TGTGGACATG CTGAGCAAAG AGATTCAGGA TCTTCAGAAC
    AAGCCTGATC GTACAGCTGA AGAGAACGAC CGCCTGCGCA AACTCATGCT AGAGTGGCAG
    TTCCAAAAGC GACTTCAGGA ATCAAAACAA AAGGATGAAG ATGATGATGA AGAAGAGGAC
    GATGATGTGG ATACTATGCT TATCATGCAG CGGCTGGAGG CAGAAAGAAG AGCAAGGCAG
    ACTGCTGTGC CAGCAATCTC TGTTCTAGAC TTGTTACAGG ATGAAGAACG CAGACGACAG
    CAACAGTTAG AAGAAATGCG CAAGCGAGAA GCAGAAGACA GGGCCAGGCA GGAAGAGGAG
    CGCCGACAGC AAGAGGAGGA GCGAACCAGG AGAGAGGCAG AAGAAAAGAG GCGACAGGAA
    GAGGAGTATT ACAATCGCTT AGAGGCTGAA AGGCGCAGGC AGCACGATGA AGCAGAACGA
    AGATTGCTGG AACCAGACGA GCCTGGTCTG TACAGACCTC CACTTCCACG GGAATATGAA
    CTCCCATCCC CAACCCCTTC ATCTAACGCT CCTCCTCCCC CACCTCAGCG AAACACCTCT
    TACCTCAAAA CACAGGTCCT CTCCCCTGAC ACGATTTATA CTGCCAAGTT TGTTGCATAC
    AATGATGATG AGGAGGAGGA GGATTCCAAT CTAGCAGGTC AGGATAAGTA CTCCAGCACA
    AGAAAATCTT ATGGTGACTT TCCTCCTGCC ACCCTTAAAC CACAGCAGCC CTCCCGCCAG
    CCTCGCCCAG TTTCAGATGG CCTCTTTCTT TCCAACTCAT TCCAGTCATC TCCAGTCAAT
    GCCAACAGTA CTGCTGCCAA AAAAAGCCAG CCCCCGCCCC CACCAAACAA ACCGAGTTTC
    ATCCATGCCA GCAGCTCTAA AGGAATAAAT TCATACACTG GATCTTCGGG AGCAACGGTT
    GGGGCCCATG AAGTCTATCG GGATCCAAGA GACAAACGAT CTAAAAGTCA AGATACAGAT
    TTACCTGGAG CACCAGAAAA TCTGACGTTC AGGGAACGCC AGCGACTTTT TTCACAGGGT
    CAAGATGTAT CCAATAAAGT AAAAGCCTCT AGGAAACTAA TGGAACTGGA AAATGAGTTG
    AACACGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33803
    Clone ID Related Accession (Same CDS sequence) XM_015284353.2 , XM_015284353.1
    Accession Version XM_015284353.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5712bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAAGAA GATTAGATGT GGATGAGAAG CCTCTTGTTG TACAATTAAA CTGGAACAAA
    GATGATCGAG AAGGAAGGTT TGTTCTCAAG AATGAAAATG ATACGCTTCC TCCAAAGAAG
    GCTCAGAGTA ATGGCCCTGA AAAACAAGAA AAAGAAGGAG TAATACAGAA TTTCAAACGA
    ACTCTCTCAA AGAAAGAAAA GAAAGAGAAG AAGAGGCGTG AAAAAGAGGC ATTGCGACAA
    GCATCAGATA AAGATGATCG ACTTCTTCAT GGGGATGATA TTGAGAATTC TCGCCTTGCA
    GCTGAGGTTT ACAAAGATAT GCCTGAGACC AGCTTCACTC GCACAATATC TAATCCTGAG
    GTGGTTATGA AACGACGGCG GCAGCAAAAA CTAGAGAAGA GAATGCAGGA ATTTCGCAGT
    TCTGATGGCA GACCAGACTC AGGTGGGACG CTGAGAATTT ATGCGGACAG TTTAAAACCA
    AATATACCAT ACAAGACAAT CCTGTTGTCA ACTACAGATA CTGCAGACTT TGCAGTTATT
    GAAGCACTGG AGAAATATGG CTTGGAGAAG GAAAACCCCA AGGATTATTG TATTGCTCGT
    GTCATTCTGC CTCCTGGTGC TCAGCACTCT GATGATAAAG GTGCTAAAGA AACTATTCTA
    GATGATGATG AGTGTCCACT TCAGATATTC AGGGAATGGC CTAGTGATAA AGGAATACTA
    GTCTTTCAGT TGAAAAGGCG GCCTCCTGAT TATGTCCCAA AGAAATCCAA GAAAATAGCT
    GATGGAAAGC CATTAAAGGG GAAGGAAAGA GTTGACGGGT CTGGCTACGG CTCTTGCCTT
    CCTCCTGAGA AACTACCATA CCTGGTAGAA TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACGCTTA TGAAGATGGT TCTGACTCCA GAGATAAACC AAAGCTTTAT
    CGTCTACAAT TAAGTGTCAC TGAAGTTGGA ACTGAAAAAT TTGATGACAA TTCCATTCAG
    TTATTTGGTC CAGGAATTCA GCCTCATCAT TGTGATCTTA CTAATATGGA TGGAGTTGTT
    ACTGTAACCC CAAGAAACAT TGATGCGGAA ACGTATGTAG AAGGTCAGCG TATTTCAGAA
    ACCACTATGC TGCAAAGTGG AATGAAGGTT CAGTTTGGGT CATCTCATGT GTTTAAGTTT
    GTGGATCCCA CTCAGGACCA TATTATTACT AAAAGACCTA TTGATGCAAG CCTTATGGTC
    AAAGGTCCAA GGCATAAAGC TGGAGCTGTT CAGGAAACCA CGTTTGATCT GGAGGGAGAT
    GTTCACAGTG GAACAGCGTT ACCAGCGAGC AAGAGCACCA GCAGGCTTGA TGGTGACAGA
    ACATCATCAG CATCCAGCAC AGCTGAACGA GGAATGGTGA AGCCCATGAT TAGAATAGAA
    CAGCAGCAAG ATTATCGCAG ACAGGAAAGC AGACCTCAGG ATACCCATGG ACCAGAACTG
    ATCCTACCTG CCAGTATTGA ATTCAGGGAA AGCTCTGAAG ATGCCTTCTT ATCTGCCATT
    ATAAATTATA CAAATAGCTC GACAGTTCAT TTTAAACTGT CACCCACGTA TGTTTTGTAT
    ATGACATGTC GGTATGTATT GTCAAGCCAG TACAGACCTG ACATCACTCC TACTGAGCGT
    ACTCACAAAG TTATTGCAAT AGTCAACAAG ATGGTGAATA TGATGGAAGG AGTCATACAG
    AAACAGAAGA ATATTGCTGG GGCACTTGCC TTTTGGATGG CAAATGCGTC TGAATTGCTG
    AATTTTATAA AGCAAGACCG TGATCTGAGC CGAATCACTG TGGATGCTCA AGATGTTTTG
    GCGCACTTGG TTCAAATGGC ATTCAAGTAC CTAGTTCATT GTCTGCAGTC AGAGCTTAAT
    AACTACATGC CAGCATTTCT TGATGATCCA GAGGAAAATA ATCCACAGAG GCCTAAAATA
    GATGATGTGC TGCACACATT GACTGGAGCT ATGTCCTTGT TGCGACGATG TAGAGTGAAT
    GCTGCACTGA CTATACAGCT CTTCTCCCAG TTGTTTCATT TTATCAACAT GTGGCTTTTT
    AACAGACTAG TTACGGCTCC AGATTCAGGG TTATGTTCCC ACTACTGGGG AGCTATTGTT
    CGTCAGCAGT TGGGTCATAT TGAGGCCTGG GCAGAAAAAC AGGGTTTAGA ATTGGCTGCT
    GATTGCCACT TAAGCAGAAT AGTGCAGGCA ACCACATTGC TCACCATGGA CAAGTACTCA
    CATGCTGATG TTCCAAATAT TAACAGTACT TGCTTTAAGT TGAATTCTCT ACAGCTACAA
    GCTTTGTTGC AGAATTATCA CTGTGCTCCA GATGAACCGC TTATACCAAC AGAACTGATA
    GAGAATGTAG TAACTGTTGC AGAAAATACG GCTGATGAAC TTGCTCGCAG TGATGGCAGA
    GAAGTCCAAT TGGAAGAGGA TCCTGACTTA CAACTCCCTT TTCTTTTACC AGAAGATGGC
    TATTCCTGTG ATGTTGTCAG AAATGTTCCA AGTGGTTTAC AAGAATTTTT GGATCCCCTC
    TGTCAGAGAG GTTTTTGTAG GTTAATACCT CATACACGGT CACCAGGCAC ATGGACAATA
    TACTTTGAAG GCGCAGACTA TGAAAGCCAC CTGTCACATG AGAATGCTGA GCTAGCTCAG
    CCTTTGAGGA AAGAACCTGA GATTATAACT GTAACACTAA AAAAACAAAA TGGAATGGGC
    CTCAGCATTG TTGCTGCCAA GGGTGCTGGT CAAGACAAAC TTGGAATATA TGTCAAGTCT
    GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGTCGTCTGG CAGCAGGGGA TCAGCTGCTC
    AGTGTGGATG GTCGAAGCTT GGTGGGGCTG TCCCAGGAAA GGGCAGCAGA ACTTATGACG
    AGGACAGGTT CTGTTGTAAC ACTGGAAGTA GCAAAACAAG GAGCAATATA CCACGGTCTG
    GCAACCCTGC TAAATCAGCC ATCTCCAATG ATGCAAAGAG TTTCTGACCG CCGTGGCTCA
    GGTAAACCCC GACCAAAGAG TGAAGGTTTT GAGCTGTATA ACAACTCCAC GCAAAATGGG
    TCACCTGAGA GCCCCCAGCT GCCATGGACG GAGTACAGTG AACCAAAGAA GCTGCCAGGA
    GATGACAGAT TAATGAAGAA CAGAGCCGAT CATCGCTCCA GCCCCAACGT AGCAAATCAG
    CCTCCAAGCC CTGGGGGGAA GAATGCTTAT GCATCTGGAA CTACCACCAA AATAACCTCA
    GTCTCGACTG GAAATCTTTG TACAGAGGAG CAAACTCTGC CTCCACGACC TGAAGCTTAT
    CCTATCCCAA CACAGACCTA TACCAGAGAA TATTTCACAT TCCCAGCTTC AAAATCCCAG
    GATCGAATGG GTCCAGCTCA GAGTCAGTGG CCAAATTATG AAGAGAAGCC ACAGATCCAG
    GCAGAAAGTA ATCACTCAAT CAATACTACA ATGCAGCGCG TGGCTCGATC CCAGGAAGAA
    CTCCGAGATA AAGTTTTCCA GCCAGAGAGG AATCGTTTGG AAGTTGTTAT CCGGAAGGAC
    ATAGAATACA TTGATCGGAA GTCAGACAGC GATCAGTGGA TGAATTCATC TGTGGATTCT
    AGCACATCTA GCCAAGAGCA CCTGAACCAT ACATCCAAAC CTGTTTCACC TGGTTCTTGC
    TTAACTAAAA GTGGACCTGG CCGTTGGAAA ACTCCAGCTA CGAGCCAGCC AACTCCAGTG
    GCCGTTTCCC AGCCCATCAG AACAGACCTT CCTCCTCCGC CGCCACCTCC TCCGGTGCAT
    TATGCAGCTG AATTTGATGA ACCACAAATG GATTTGCCTC TGCCTCCCCC TCCTCCACCG
    AACCAAGTAG GAGCACAAGC ATCTTCTCAG GTGGCTGCTG CTGAGCGTAA AAAACGAGAA
    GAGCATCAGA GGTGGTATGA AAAGGAAAAG GCACGTTTGG AAGAAGAACG AGAAAGGAAA
    CGCCGTGAGC AAGAGAGAAA ACTGGGCCAA ATGCGTTCGC AGCCACTAAT TCCTCCCCAG
    CCTGTGGTTC CTCCTGTTTC CCTTCAGCAA GTGAAATCGG AAAAAACTTC AACTTTCCAG
    AGGTCTCAAG AAACAGTCAT TCGAGAGTTG CAGCCGCAAC AGCAGCCTCG GACTATTGAG
    CGGAGAGATC TGCAGTACAT TACTATCAGC AAGGAAGAAT TGTCCTCTAG TGACAGCCTC
    TCTCCAGATC CCTGGAAACG AGATGCCAAG GAGAAACTGG AGAAGCAGCA GCAGATGCAC
    ATTGTGGACA TGCTGAGCAA AGAGATTCAG GATCTTCAGA ACAAGCCTGA TCGTACAGCT
    GAAGAGAACG ACCGCCTGCG CAAACTCATG CTAGAGTGGC AGTTCCAAAA GCGACTTCAG
    GAATCAAAAC AAAAGGATGA AGATGATGAT GAAGAAGAGG ACGATGATGT GGATACTATG
    CTTATCATGC AGCGGCTGGA GGCAGAAAGA AGAGCAAGGG ATGAAGAACG CAGACGACAG
    CAACAGTTAG AAGAAATGCG CAAGCGAGAA GCAGAAGACA GGGCCAGGCA GGAAGAGGAG
    CGCCGACAGC AAGAGGAGGA GCGAACCAGG AGAGAGGCAG AAGAAAAGAG GCGACAGGAA
    GAGGAGTATT ACAATCGCTT AGAGGCTGAA AGGCGCAGGC AGCACGATGA AGCAGAACGA
    AGATTGCTGG AACCAGACGA GCCTGGTCTG TACAGACCTC CACTTCCACG GGAATATGAA
    CTCCCATCCC CAACCCCTTC ATCTAACGCT CCTCCTCCCC CACCTCAGCG AAACACCTCT
    TACCTCAAAA CACAGGTCCT CTCCCCTGAC ACGATTTATA CTGCCAAGTT TGTTGCATAC
    AATGATGATG AGGAGGAGGA GGATTCCAAT CTAGCAGGTC AGGATAAGTA CTCCAGCACA
    AGAAAATCTT ATGGTGACTT TCCTCCTGCC ACCCTTAAAC CACAGCAGCC CTCCCGCCAG
    CCTCGCCCAG TTTCAGATGG CCTCTTTCTT TCCAACTCAT TCCAGTCATC TCCAGTCAAT
    GCCAACAGTA CTGCTGCCAA AAAAAGCCAG CCCCCGCCCC CACCAAACAA ACCGAGTTTC
    ATCCATGCCA GCAGCTCTAA AGGAATAAAT TCATACACTG GATCTTCGGG AGCAACGGTT
    GGGGCCCATG AAGTCTATCG GGATCCAAGA GACAAACGAT CTAAAAGTCA AGATACAGAT
    TTACCTGGAG CACCAGAAAA TCTGACGTTC AGGGAACGCC AGCGACTTTT TTCACAGGGT
    CAAGATGTAT CCAATAAAGT AAAAGCCTCT AGGAAACTAA TGGAACTGGA AAATGAGTTG
    AACACGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139839.1
    CDS2885..8596
    Translation

    Target ORF information:

    RefSeq Version XM_015284353.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284353.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAAGAA GATTAGATGT GGATGAGAAG CCTCTTGTTG TACAATTAAA CTGGAACAAA
    GATGATCGAG AAGGAAGGTT TGTTCTCAAG AATGAAAATG ATACGCTTCC TCCAAAGAAG
    GCTCAGAGTA ATGGCCCTGA AAAACAAGAA AAAGAAGGAG TAATACAGAA TTTCAAACGA
    ACTCTCTCAA AGAAAGAAAA GAAAGAGAAG AAGAGGCGTG AAAAAGAGGC ATTGCGACAA
    GCATCAGATA AAGATGATCG ACTTCTTCAT GGGGATGATA TTGAGAATTC TCGCCTTGCA
    GCTGAGGTTT ACAAAGATAT GCCTGAGACC AGCTTCACTC GCACAATATC TAATCCTGAG
    GTGGTTATGA AACGACGGCG GCAGCAAAAA CTAGAGAAGA GAATGCAGGA ATTTCGCAGT
    TCTGATGGCA GACCAGACTC AGGTGGGACG CTGAGAATTT ATGCGGACAG TTTAAAACCA
    AATATACCAT ACAAGACAAT CCTGTTGTCA ACTACAGATA CTGCAGACTT TGCAGTTATT
    GAAGCACTGG AGAAATATGG CTTGGAGAAG GAAAACCCCA AGGATTATTG TATTGCTCGT
    GTCATTCTGC CTCCTGGTGC TCAGCACTCT GATGATAAAG GTGCTAAAGA AACTATTCTA
    GATGATGATG AGTGTCCACT TCAGATATTC AGGGAATGGC CTAGTGATAA AGGAATACTA
    GTCTTTCAGT TGAAAAGGCG GCCTCCTGAT TATGTCCCAA AGAAATCCAA GAAAATAGCT
    GATGGAAAGC CATTAAAGGG GAAGGAAAGA GTTGACGGGT CTGGCTACGG CTCTTGCCTT
    CCTCCTGAGA AACTACCATA CCTGGTAGAA TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACGCTTA TGAAGATGGT TCTGACTCCA GAGATAAACC AAAGCTTTAT
    CGTCTACAAT TAAGTGTCAC TGAAGTTGGA ACTGAAAAAT TTGATGACAA TTCCATTCAG
    TTATTTGGTC CAGGAATTCA GCCTCATCAT TGTGATCTTA CTAATATGGA TGGAGTTGTT
    ACTGTAACCC CAAGAAACAT TGATGCGGAA ACGTATGTAG AAGGTCAGCG TATTTCAGAA
    ACCACTATGC TGCAAAGTGG AATGAAGGTT CAGTTTGGGT CATCTCATGT GTTTAAGTTT
    GTGGATCCCA CTCAGGACCA TATTATTACT AAAAGACCTA TTGATGCAAG CCTTATGGTC
    AAAGGTCCAA GGCATAAAGC TGGAGCTGTT CAGGAAACCA CGTTTGATCT GGAGGGAGAT
    GTTCACAGTG GAACAGCGTT ACCAGCGAGC AAGAGCACCA GCAGGCTTGA TGGTGACAGA
    ACATCATCAG CATCCAGCAC AGCTGAACGA GGAATGGTGA AGCCCATGAT TAGAATAGAA
    CAGCAGCAAG ATTATCGCAG ACAGGAAAGC AGACCTCAGG ATACCCATGG ACCAGAACTG
    ATCCTACCTG CCAGTATTGA ATTCAGGGAA AGCTCTGAAG ATGCCTTCTT ATCTGCCATT
    ATAAATTATA CAAATAGCTC GACAGTTCAT TTTAAACTGT CACCCACGTA TGTTTTGTAT
    ATGACATGTC GGTATGTATT GTCAAGCCAG TACAGACCTG ACATCACTCC TACTGAGCGT
    ACTCACAAAG TTATTGCAAT AGTCAACAAG ATGGTGAATA TGATGGAAGG AGTCATACAG
    AAACAGAAGA ATATTGCTGG GGCACTTGCC TTTTGGATGG CAAATGCGTC TGAATTGCTG
    AATTTTATAA AGCAAGACCG TGATCTGAGC CGAATCACTG TGGATGCTCA AGATGTTTTG
    GCGCACTTGG TTCAAATGGC ATTCAAGTAC CTAGTTCATT GTCTGCAGTC AGAGCTTAAT
    AACTACATGC CAGCATTTCT TGATGATCCA GAGGAAAATA ATCCACAGAG GCCTAAAATA
    GATGATGTGC TGCACACATT GACTGGAGCT ATGTCCTTGT TGCGACGATG TAGAGTGAAT
    GCTGCACTGA CTATACAGCT CTTCTCCCAG TTGTTTCATT TTATCAACAT GTGGCTTTTT
    AACAGACTAG TTACGGCTCC AGATTCAGGG TTATGTTCCC ACTACTGGGG AGCTATTGTT
    CGTCAGCAGT TGGGTCATAT TGAGGCCTGG GCAGAAAAAC AGGGTTTAGA ATTGGCTGCT
    GATTGCCACT TAAGCAGAAT AGTGCAGGCA ACCACATTGC TCACCATGGA CAAGTACTCA
    CATGCTGATG TTCCAAATAT TAACAGTACT TGCTTTAAGT TGAATTCTCT ACAGCTACAA
    GCTTTGTTGC AGAATTATCA CTGTGCTCCA GATGAACCGC TTATACCAAC AGAACTGATA
    GAGAATGTAG TAACTGTTGC AGAAAATACG GCTGATGAAC TTGCTCGCAG TGATGGCAGA
    GAAGTCCAAT TGGAAGAGGA TCCTGACTTA CAACTCCCTT TTCTTTTACC AGAAGATGGC
    TATTCCTGTG ATGTTGTCAG AAATGTTCCA AGTGGTTTAC AAGAATTTTT GGATCCCCTC
    TGTCAGAGAG GTTTTTGTAG GTTAATACCT CATACACGGT CACCAGGCAC ATGGACAATA
    TACTTTGAAG GCGCAGACTA TGAAAGCCAC CTGTCACATG AGAATGCTGA GCTAGCTCAG
    CCTTTGAGGA AAGAACCTGA GATTATAACT GTAACACTAA AAAAACAAAA TGGAATGGGC
    CTCAGCATTG TTGCTGCCAA GGGTGCTGGT CAAGACAAAC TTGGAATATA TGTCAAGTCT
    GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGTCGTCTGG CAGCAGGGGA TCAGCTGCTC
    AGTGTGGATG GTCGAAGCTT GGTGGGGCTG TCCCAGGAAA GGGCAGCAGA ACTTATGACG
    AGGACAGGTT CTGTTGTAAC ACTGGAAGTA GCAAAACAAG GAGCAATATA CCACGGTCTG
    GCAACCCTGC TAAATCAGCC ATCTCCAATG ATGCAAAGAG TTTCTGACCG CCGTGGCTCA
    GGTAAACCCC GACCAAAGAG TGAAGGTTTT GAGCTGTATA ACAACTCCAC GCAAAATGGG
    TCACCTGAGA GCCCCCAGCT GCCATGGACG GAGTACAGTG AACCAAAGAA GCTGCCAGGA
    GATGACAGAT TAATGAAGAA CAGAGCCGAT CATCGCTCCA GCCCCAACGT AGCAAATCAG
    CCTCCAAGCC CTGGGGGGAA GAATGCTTAT GCATCTGGAA CTACCACCAA AATAACCTCA
    GTCTCGACTG GAAATCTTTG TACAGAGGAG CAAACTCTGC CTCCACGACC TGAAGCTTAT
    CCTATCCCAA CACAGACCTA TACCAGAGAA TATTTCACAT TCCCAGCTTC AAAATCCCAG
    GATCGAATGG GTCCAGCTCA GAGTCAGTGG CCAAATTATG AAGAGAAGCC ACAGATCCAG
    GCAGAAAGTA ATCACTCAAT CAATACTACA ATGCAGCGCG TGGCTCGATC CCAGGAAGAA
    CTCCGAGATA AAGTTTTCCA GCCAGAGAGG AATCGTTTGG AAGTTGTTAT CCGGAAGGAC
    ATAGAATACA TTGATCGGAA GTCAGACAGC GATCAGTGGA TGAATTCATC TGTGGATTCT
    AGCACATCTA GCCAAGAGCA CCTGAACCAT ACATCCAAAC CTGTTTCACC TGGTTCTTGC
    TTAACTAAAA GTGGACCTGG CCGTTGGAAA ACTCCAGCTA CGAGCCAGCC AACTCCAGTG
    GCCGTTTCCC AGCCCATCAG AACAGACCTT CCTCCTCCGC CGCCACCTCC TCCGGTGCAT
    TATGCAGCTG AATTTGATGA ACCACAAATG GATTTGCCTC TGCCTCCCCC TCCTCCACCG
    AACCAAGTAG GAGCACAAGC ATCTTCTCAG GTGGCTGCTG CTGAGCGTAA AAAACGAGAA
    GAGCATCAGA GGTGGTATGA AAAGGAAAAG GCACGTTTGG AAGAAGAACG AGAAAGGAAA
    CGCCGTGAGC AAGAGAGAAA ACTGGGCCAA ATGCGTTCGC AGCCACTAAT TCCTCCCCAG
    CCTGTGGTTC CTCCTGTTTC CCTTCAGCAA GTGAAATCGG AAAAAACTTC AACTTTCCAG
    AGGTCTCAAG AAACAGTCAT TCGAGAGTTG CAGCCGCAAC AGCAGCCTCG GACTATTGAG
    CGGAGAGATC TGCAGTACAT TACTATCAGC AAGGAAGAAT TGTCCTCTAG TGACAGCCTC
    TCTCCAGATC CCTGGAAACG AGATGCCAAG GAGAAACTGG AGAAGCAGCA GCAGATGCAC
    ATTGTGGACA TGCTGAGCAA AGAGATTCAG GATCTTCAGA ACAAGCCTGA TCGTACAGCT
    GAAGAGAACG ACCGCCTGCG CAAACTCATG CTAGAGTGGC AGTTCCAAAA GCGACTTCAG
    GAATCAAAAC AAAAGGATGA AGATGATGAT GAAGAAGAGG ACGATGATGT GGATACTATG
    CTTATCATGC AGCGGCTGGA GGCAGAAAGA AGAGCAAGGG ATGAAGAACG CAGACGACAG
    CAACAGTTAG AAGAAATGCG CAAGCGAGAA GCAGAAGACA GGGCCAGGCA GGAAGAGGAG
    CGCCGACAGC AAGAGGAGGA GCGAACCAGG AGAGAGGCAG AAGAAAAGAG GCGACAGGAA
    GAGGAGTATT ACAATCGCTT AGAGGCTGAA AGGCGCAGGC AGCACGATGA AGCAGAACGA
    AGATTGCTGG AACCAGACGA GCCTGGTCTG TACAGACCTC CACTTCCACG GGAATATGAA
    CTCCCATCCC CAACCCCTTC ATCTAACGCT CCTCCTCCCC CACCTCAGCG AAACACCTCT
    TACCTCAAAA CACAGGTCCT CTCCCCTGAC ACGATTTATA CTGCCAAGTT TGTTGCATAC
    AATGATGATG AGGAGGAGGA GGATTCCAAT CTAGCAGGTC AGGATAAGTA CTCCAGCACA
    AGAAAATCTT ATGGTGACTT TCCTCCTGCC ACCCTTAAAC CACAGCAGCC CTCCCGCCAG
    CCTCGCCCAG TTTCAGATGG CCTCTTTCTT TCCAACTCAT TCCAGTCATC TCCAGTCAAT
    GCCAACAGTA CTGCTGCCAA AAAAAGCCAG CCCCCGCCCC CACCAAACAA ACCGAGTTTC
    ATCCATGCCA GCAGCTCTAA AGGAATAAAT TCATACACTG GATCTTCGGG AGCAACGGTT
    GGGGCCCATG AAGTCTATCG GGATCCAAGA GACAAACGAT CTAAAAGTCA AGATACAGAT
    TTACCTGGAG CACCAGAAAA TCTGACGTTC AGGGAACGCC AGCGACTTTT TTCACAGGGT
    CAAGATGTAT CCAATAAAGT AAAAGCCTCT AGGAAACTAA TGGAACTGGA AAATGAGTTG
    AACACGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33803
    Clone ID Related Accession (Same CDS sequence) XM_015284353.2 , XM_015284353.1
    Accession Version XM_015284353.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5712bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAAGAA GATTAGATGT GGATGAGAAG CCTCTTGTTG TACAATTAAA CTGGAACAAA
    GATGATCGAG AAGGAAGGTT TGTTCTCAAG AATGAAAATG ATACGCTTCC TCCAAAGAAG
    GCTCAGAGTA ATGGCCCTGA AAAACAAGAA AAAGAAGGAG TAATACAGAA TTTCAAACGA
    ACTCTCTCAA AGAAAGAAAA GAAAGAGAAG AAGAGGCGTG AAAAAGAGGC ATTGCGACAA
    GCATCAGATA AAGATGATCG ACTTCTTCAT GGGGATGATA TTGAGAATTC TCGCCTTGCA
    GCTGAGGTTT ACAAAGATAT GCCTGAGACC AGCTTCACTC GCACAATATC TAATCCTGAG
    GTGGTTATGA AACGACGGCG GCAGCAAAAA CTAGAGAAGA GAATGCAGGA ATTTCGCAGT
    TCTGATGGCA GACCAGACTC AGGTGGGACG CTGAGAATTT ATGCGGACAG TTTAAAACCA
    AATATACCAT ACAAGACAAT CCTGTTGTCA ACTACAGATA CTGCAGACTT TGCAGTTATT
    GAAGCACTGG AGAAATATGG CTTGGAGAAG GAAAACCCCA AGGATTATTG TATTGCTCGT
    GTCATTCTGC CTCCTGGTGC TCAGCACTCT GATGATAAAG GTGCTAAAGA AACTATTCTA
    GATGATGATG AGTGTCCACT TCAGATATTC AGGGAATGGC CTAGTGATAA AGGAATACTA
    GTCTTTCAGT TGAAAAGGCG GCCTCCTGAT TATGTCCCAA AGAAATCCAA GAAAATAGCT
    GATGGAAAGC CATTAAAGGG GAAGGAAAGA GTTGACGGGT CTGGCTACGG CTCTTGCCTT
    CCTCCTGAGA AACTACCATA CCTGGTAGAA TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACGCTTA TGAAGATGGT TCTGACTCCA GAGATAAACC AAAGCTTTAT
    CGTCTACAAT TAAGTGTCAC TGAAGTTGGA ACTGAAAAAT TTGATGACAA TTCCATTCAG
    TTATTTGGTC CAGGAATTCA GCCTCATCAT TGTGATCTTA CTAATATGGA TGGAGTTGTT
    ACTGTAACCC CAAGAAACAT TGATGCGGAA ACGTATGTAG AAGGTCAGCG TATTTCAGAA
    ACCACTATGC TGCAAAGTGG AATGAAGGTT CAGTTTGGGT CATCTCATGT GTTTAAGTTT
    GTGGATCCCA CTCAGGACCA TATTATTACT AAAAGACCTA TTGATGCAAG CCTTATGGTC
    AAAGGTCCAA GGCATAAAGC TGGAGCTGTT CAGGAAACCA CGTTTGATCT GGAGGGAGAT
    GTTCACAGTG GAACAGCGTT ACCAGCGAGC AAGAGCACCA GCAGGCTTGA TGGTGACAGA
    ACATCATCAG CATCCAGCAC AGCTGAACGA GGAATGGTGA AGCCCATGAT TAGAATAGAA
    CAGCAGCAAG ATTATCGCAG ACAGGAAAGC AGACCTCAGG ATACCCATGG ACCAGAACTG
    ATCCTACCTG CCAGTATTGA ATTCAGGGAA AGCTCTGAAG ATGCCTTCTT ATCTGCCATT
    ATAAATTATA CAAATAGCTC GACAGTTCAT TTTAAACTGT CACCCACGTA TGTTTTGTAT
    ATGACATGTC GGTATGTATT GTCAAGCCAG TACAGACCTG ACATCACTCC TACTGAGCGT
    ACTCACAAAG TTATTGCAAT AGTCAACAAG ATGGTGAATA TGATGGAAGG AGTCATACAG
    AAACAGAAGA ATATTGCTGG GGCACTTGCC TTTTGGATGG CAAATGCGTC TGAATTGCTG
    AATTTTATAA AGCAAGACCG TGATCTGAGC CGAATCACTG TGGATGCTCA AGATGTTTTG
    GCGCACTTGG TTCAAATGGC ATTCAAGTAC CTAGTTCATT GTCTGCAGTC AGAGCTTAAT
    AACTACATGC CAGCATTTCT TGATGATCCA GAGGAAAATA ATCCACAGAG GCCTAAAATA
    GATGATGTGC TGCACACATT GACTGGAGCT ATGTCCTTGT TGCGACGATG TAGAGTGAAT
    GCTGCACTGA CTATACAGCT CTTCTCCCAG TTGTTTCATT TTATCAACAT GTGGCTTTTT
    AACAGACTAG TTACGGCTCC AGATTCAGGG TTATGTTCCC ACTACTGGGG AGCTATTGTT
    CGTCAGCAGT TGGGTCATAT TGAGGCCTGG GCAGAAAAAC AGGGTTTAGA ATTGGCTGCT
    GATTGCCACT TAAGCAGAAT AGTGCAGGCA ACCACATTGC TCACCATGGA CAAGTACTCA
    CATGCTGATG TTCCAAATAT TAACAGTACT TGCTTTAAGT TGAATTCTCT ACAGCTACAA
    GCTTTGTTGC AGAATTATCA CTGTGCTCCA GATGAACCGC TTATACCAAC AGAACTGATA
    GAGAATGTAG TAACTGTTGC AGAAAATACG GCTGATGAAC TTGCTCGCAG TGATGGCAGA
    GAAGTCCAAT TGGAAGAGGA TCCTGACTTA CAACTCCCTT TTCTTTTACC AGAAGATGGC
    TATTCCTGTG ATGTTGTCAG AAATGTTCCA AGTGGTTTAC AAGAATTTTT GGATCCCCTC
    TGTCAGAGAG GTTTTTGTAG GTTAATACCT CATACACGGT CACCAGGCAC ATGGACAATA
    TACTTTGAAG GCGCAGACTA TGAAAGCCAC CTGTCACATG AGAATGCTGA GCTAGCTCAG
    CCTTTGAGGA AAGAACCTGA GATTATAACT GTAACACTAA AAAAACAAAA TGGAATGGGC
    CTCAGCATTG TTGCTGCCAA GGGTGCTGGT CAAGACAAAC TTGGAATATA TGTCAAGTCT
    GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGTCGTCTGG CAGCAGGGGA TCAGCTGCTC
    AGTGTGGATG GTCGAAGCTT GGTGGGGCTG TCCCAGGAAA GGGCAGCAGA ACTTATGACG
    AGGACAGGTT CTGTTGTAAC ACTGGAAGTA GCAAAACAAG GAGCAATATA CCACGGTCTG
    GCAACCCTGC TAAATCAGCC ATCTCCAATG ATGCAAAGAG TTTCTGACCG CCGTGGCTCA
    GGTAAACCCC GACCAAAGAG TGAAGGTTTT GAGCTGTATA ACAACTCCAC GCAAAATGGG
    TCACCTGAGA GCCCCCAGCT GCCATGGACG GAGTACAGTG AACCAAAGAA GCTGCCAGGA
    GATGACAGAT TAATGAAGAA CAGAGCCGAT CATCGCTCCA GCCCCAACGT AGCAAATCAG
    CCTCCAAGCC CTGGGGGGAA GAATGCTTAT GCATCTGGAA CTACCACCAA AATAACCTCA
    GTCTCGACTG GAAATCTTTG TACAGAGGAG CAAACTCTGC CTCCACGACC TGAAGCTTAT
    CCTATCCCAA CACAGACCTA TACCAGAGAA TATTTCACAT TCCCAGCTTC AAAATCCCAG
    GATCGAATGG GTCCAGCTCA GAGTCAGTGG CCAAATTATG AAGAGAAGCC ACAGATCCAG
    GCAGAAAGTA ATCACTCAAT CAATACTACA ATGCAGCGCG TGGCTCGATC CCAGGAAGAA
    CTCCGAGATA AAGTTTTCCA GCCAGAGAGG AATCGTTTGG AAGTTGTTAT CCGGAAGGAC
    ATAGAATACA TTGATCGGAA GTCAGACAGC GATCAGTGGA TGAATTCATC TGTGGATTCT
    AGCACATCTA GCCAAGAGCA CCTGAACCAT ACATCCAAAC CTGTTTCACC TGGTTCTTGC
    TTAACTAAAA GTGGACCTGG CCGTTGGAAA ACTCCAGCTA CGAGCCAGCC AACTCCAGTG
    GCCGTTTCCC AGCCCATCAG AACAGACCTT CCTCCTCCGC CGCCACCTCC TCCGGTGCAT
    TATGCAGCTG AATTTGATGA ACCACAAATG GATTTGCCTC TGCCTCCCCC TCCTCCACCG
    AACCAAGTAG GAGCACAAGC ATCTTCTCAG GTGGCTGCTG CTGAGCGTAA AAAACGAGAA
    GAGCATCAGA GGTGGTATGA AAAGGAAAAG GCACGTTTGG AAGAAGAACG AGAAAGGAAA
    CGCCGTGAGC AAGAGAGAAA ACTGGGCCAA ATGCGTTCGC AGCCACTAAT TCCTCCCCAG
    CCTGTGGTTC CTCCTGTTTC CCTTCAGCAA GTGAAATCGG AAAAAACTTC AACTTTCCAG
    AGGTCTCAAG AAACAGTCAT TCGAGAGTTG CAGCCGCAAC AGCAGCCTCG GACTATTGAG
    CGGAGAGATC TGCAGTACAT TACTATCAGC AAGGAAGAAT TGTCCTCTAG TGACAGCCTC
    TCTCCAGATC CCTGGAAACG AGATGCCAAG GAGAAACTGG AGAAGCAGCA GCAGATGCAC
    ATTGTGGACA TGCTGAGCAA AGAGATTCAG GATCTTCAGA ACAAGCCTGA TCGTACAGCT
    GAAGAGAACG ACCGCCTGCG CAAACTCATG CTAGAGTGGC AGTTCCAAAA GCGACTTCAG
    GAATCAAAAC AAAAGGATGA AGATGATGAT GAAGAAGAGG ACGATGATGT GGATACTATG
    CTTATCATGC AGCGGCTGGA GGCAGAAAGA AGAGCAAGGG ATGAAGAACG CAGACGACAG
    CAACAGTTAG AAGAAATGCG CAAGCGAGAA GCAGAAGACA GGGCCAGGCA GGAAGAGGAG
    CGCCGACAGC AAGAGGAGGA GCGAACCAGG AGAGAGGCAG AAGAAAAGAG GCGACAGGAA
    GAGGAGTATT ACAATCGCTT AGAGGCTGAA AGGCGCAGGC AGCACGATGA AGCAGAACGA
    AGATTGCTGG AACCAGACGA GCCTGGTCTG TACAGACCTC CACTTCCACG GGAATATGAA
    CTCCCATCCC CAACCCCTTC ATCTAACGCT CCTCCTCCCC CACCTCAGCG AAACACCTCT
    TACCTCAAAA CACAGGTCCT CTCCCCTGAC ACGATTTATA CTGCCAAGTT TGTTGCATAC
    AATGATGATG AGGAGGAGGA GGATTCCAAT CTAGCAGGTC AGGATAAGTA CTCCAGCACA
    AGAAAATCTT ATGGTGACTT TCCTCCTGCC ACCCTTAAAC CACAGCAGCC CTCCCGCCAG
    CCTCGCCCAG TTTCAGATGG CCTCTTTCTT TCCAACTCAT TCCAGTCATC TCCAGTCAAT
    GCCAACAGTA CTGCTGCCAA AAAAAGCCAG CCCCCGCCCC CACCAAACAA ACCGAGTTTC
    ATCCATGCCA GCAGCTCTAA AGGAATAAAT TCATACACTG GATCTTCGGG AGCAACGGTT
    GGGGCCCATG AAGTCTATCG GGATCCAAGA GACAAACGAT CTAAAAGTCA AGATACAGAT
    TTACCTGGAG CACCAGAAAA TCTGACGTTC AGGGAACGCC AGCGACTTTT TTCACAGGGT
    CAAGATGTAT CCAATAAAGT AAAAGCCTCT AGGAAACTAA TGGAACTGGA AAATGAGTTG
    AACACGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139839.1
    CDS70..5781
    Translation

    Target ORF information:

    RefSeq Version XM_015284353.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284353.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAAGAA GATTAGATGT GGATGAGAAG CCTCTTGTTG TACAATTAAA CTGGAACAAA
    GATGATCGAG AAGGAAGGTT TGTTCTCAAG AATGAAAATG ATACGCTTCC TCCAAAGAAG
    GCTCAGAGTA ATGGCCCTGA AAAACAAGAA AAAGAAGGAG TAATACAGAA TTTCAAACGA
    ACTCTCTCAA AGAAAGAAAA GAAAGAGAAG AAGAGGCGTG AAAAAGAGGC ATTGCGACAA
    GCATCAGATA AAGATGATCG ACTTCTTCAT GGGGATGATA TTGAGAATTC TCGCCTTGCA
    GCTGAGGTTT ACAAAGATAT GCCTGAGACC AGCTTCACTC GCACAATATC TAATCCTGAG
    GTGGTTATGA AACGACGGCG GCAGCAAAAA CTAGAGAAGA GAATGCAGGA ATTTCGCAGT
    TCTGATGGCA GACCAGACTC AGGTGGGACG CTGAGAATTT ATGCGGACAG TTTAAAACCA
    AATATACCAT ACAAGACAAT CCTGTTGTCA ACTACAGATA CTGCAGACTT TGCAGTTATT
    GAAGCACTGG AGAAATATGG CTTGGAGAAG GAAAACCCCA AGGATTATTG TATTGCTCGT
    GTCATTCTGC CTCCTGGTGC TCAGCACTCT GATGATAAAG GTGCTAAAGA AACTATTCTA
    GATGATGATG AGTGTCCACT TCAGATATTC AGGGAATGGC CTAGTGATAA AGGAATACTA
    GTCTTTCAGT TGAAAAGGCG GCCTCCTGAT TATGTCCCAA AGAAATCCAA GAAAATAGCT
    GATGGAAAGC CATTAAAGGG GAAGGAAAGA GTTGACGGGT CTGGCTACGG CTCTTGCCTT
    CCTCCTGAGA AACTACCATA CCTGGTAGAA TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACGCTTA TGAAGATGGT TCTGACTCCA GAGATAAACC AAAGCTTTAT
    CGTCTACAAT TAAGTGTCAC TGAAGTTGGA ACTGAAAAAT TTGATGACAA TTCCATTCAG
    TTATTTGGTC CAGGAATTCA GCCTCATCAT TGTGATCTTA CTAATATGGA TGGAGTTGTT
    ACTGTAACCC CAAGAAACAT TGATGCGGAA ACGTATGTAG AAGGTCAGCG TATTTCAGAA
    ACCACTATGC TGCAAAGTGG AATGAAGGTT CAGTTTGGGT CATCTCATGT GTTTAAGTTT
    GTGGATCCCA CTCAGGACCA TATTATTACT AAAAGACCTA TTGATGCAAG CCTTATGGTC
    AAAGGTCCAA GGCATAAAGC TGGAGCTGTT CAGGAAACCA CGTTTGATCT GGAGGGAGAT
    GTTCACAGTG GAACAGCGTT ACCAGCGAGC AAGAGCACCA GCAGGCTTGA TGGTGACAGA
    ACATCATCAG CATCCAGCAC AGCTGAACGA GGAATGGTGA AGCCCATGAT TAGAATAGAA
    CAGCAGCAAG ATTATCGCAG ACAGGAAAGC AGACCTCAGG ATACCCATGG ACCAGAACTG
    ATCCTACCTG CCAGTATTGA ATTCAGGGAA AGCTCTGAAG ATGCCTTCTT ATCTGCCATT
    ATAAATTATA CAAATAGCTC GACAGTTCAT TTTAAACTGT CACCCACGTA TGTTTTGTAT
    ATGACATGTC GGTATGTATT GTCAAGCCAG TACAGACCTG ACATCACTCC TACTGAGCGT
    ACTCACAAAG TTATTGCAAT AGTCAACAAG ATGGTGAATA TGATGGAAGG AGTCATACAG
    AAACAGAAGA ATATTGCTGG GGCACTTGCC TTTTGGATGG CAAATGCGTC TGAATTGCTG
    AATTTTATAA AGCAAGACCG TGATCTGAGC CGAATCACTG TGGATGCTCA AGATGTTTTG
    GCGCACTTGG TTCAAATGGC ATTCAAGTAC CTAGTTCATT GTCTGCAGTC AGAGCTTAAT
    AACTACATGC CAGCATTTCT TGATGATCCA GAGGAAAATA ATCCACAGAG GCCTAAAATA
    GATGATGTGC TGCACACATT GACTGGAGCT ATGTCCTTGT TGCGACGATG TAGAGTGAAT
    GCTGCACTGA CTATACAGCT CTTCTCCCAG TTGTTTCATT TTATCAACAT GTGGCTTTTT
    AACAGACTAG TTACGGCTCC AGATTCAGGG TTATGTTCCC ACTACTGGGG AGCTATTGTT
    CGTCAGCAGT TGGGTCATAT TGAGGCCTGG GCAGAAAAAC AGGGTTTAGA ATTGGCTGCT
    GATTGCCACT TAAGCAGAAT AGTGCAGGCA ACCACATTGC TCACCATGGA CAAGTACTCA
    CATGCTGATG TTCCAAATAT TAACAGTACT TGCTTTAAGT TGAATTCTCT ACAGCTACAA
    GCTTTGTTGC AGAATTATCA CTGTGCTCCA GATGAACCGC TTATACCAAC AGAACTGATA
    GAGAATGTAG TAACTGTTGC AGAAAATACG GCTGATGAAC TTGCTCGCAG TGATGGCAGA
    GAAGTCCAAT TGGAAGAGGA TCCTGACTTA CAACTCCCTT TTCTTTTACC AGAAGATGGC
    TATTCCTGTG ATGTTGTCAG AAATGTTCCA AGTGGTTTAC AAGAATTTTT GGATCCCCTC
    TGTCAGAGAG GTTTTTGTAG GTTAATACCT CATACACGGT CACCAGGCAC ATGGACAATA
    TACTTTGAAG GCGCAGACTA TGAAAGCCAC CTGTCACATG AGAATGCTGA GCTAGCTCAG
    CCTTTGAGGA AAGAACCTGA GATTATAACT GTAACACTAA AAAAACAAAA TGGAATGGGC
    CTCAGCATTG TTGCTGCCAA GGGTGCTGGT CAAGACAAAC TTGGAATATA TGTCAAGTCT
    GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGTCGTCTGG CAGCAGGGGA TCAGCTGCTC
    AGTGTGGATG GTCGAAGCTT GGTGGGGCTG TCCCAGGAAA GGGCAGCAGA ACTTATGACG
    AGGACAGGTT CTGTTGTAAC ACTGGAAGTA GCAAAACAAG GAGCAATATA CCACGGTCTG
    GCAACCCTGC TAAATCAGCC ATCTCCAATG ATGCAAAGAG TTTCTGACCG CCGTGGCTCA
    GGTAAACCCC GACCAAAGAG TGAAGGTTTT GAGCTGTATA ACAACTCCAC GCAAAATGGG
    TCACCTGAGA GCCCCCAGCT GCCATGGACG GAGTACAGTG AACCAAAGAA GCTGCCAGGA
    GATGACAGAT TAATGAAGAA CAGAGCCGAT CATCGCTCCA GCCCCAACGT AGCAAATCAG
    CCTCCAAGCC CTGGGGGGAA GAATGCTTAT GCATCTGGAA CTACCACCAA AATAACCTCA
    GTCTCGACTG GAAATCTTTG TACAGAGGAG CAAACTCTGC CTCCACGACC TGAAGCTTAT
    CCTATCCCAA CACAGACCTA TACCAGAGAA TATTTCACAT TCCCAGCTTC AAAATCCCAG
    GATCGAATGG GTCCAGCTCA GAGTCAGTGG CCAAATTATG AAGAGAAGCC ACAGATCCAG
    GCAGAAAGTA ATCACTCAAT CAATACTACA ATGCAGCGCG TGGCTCGATC CCAGGAAGAA
    CTCCGAGATA AAGTTTTCCA GCCAGAGAGG AATCGTTTGG AAGTTGTTAT CCGGAAGGAC
    ATAGAATACA TTGATCGGAA GTCAGACAGC GATCAGTGGA TGAATTCATC TGTGGATTCT
    AGCACATCTA GCCAAGAGCA CCTGAACCAT ACATCCAAAC CTGTTTCACC TGGTTCTTGC
    TTAACTAAAA GTGGACCTGG CCGTTGGAAA ACTCCAGCTA CGAGCCAGCC AACTCCAGTG
    GCCGTTTCCC AGCCCATCAG AACAGACCTT CCTCCTCCGC CGCCACCTCC TCCGGTGCAT
    TATGCAGCTG AATTTGATGA ACCACAAATG GATTTGCCTC TGCCTCCCCC TCCTCCACCG
    AACCAAGTAG GAGCACAAGC ATCTTCTCAG GTGGCTGCTG CTGAGCGTAA AAAACGAGAA
    GAGCATCAGA GGTGGTATGA AAAGGAAAAG GCACGTTTGG AAGAAGAACG AGAAAGGAAA
    CGCCGTGAGC AAGAGAGAAA ACTGGGCCAA ATGCGTTCGC AGCCACTAAT TCCTCCCCAG
    CCTGTGGTTC CTCCTGTTTC CCTTCAGCAA GTGAAATCGG AAAAAACTTC AACTTTCCAG
    AGGTCTCAAG AAACAGTCAT TCGAGAGTTG CAGCCGCAAC AGCAGCCTCG GACTATTGAG
    CGGAGAGATC TGCAGTACAT TACTATCAGC AAGGAAGAAT TGTCCTCTAG TGACAGCCTC
    TCTCCAGATC CCTGGAAACG AGATGCCAAG GAGAAACTGG AGAAGCAGCA GCAGATGCAC
    ATTGTGGACA TGCTGAGCAA AGAGATTCAG GATCTTCAGA ACAAGCCTGA TCGTACAGCT
    GAAGAGAACG ACCGCCTGCG CAAACTCATG CTAGAGTGGC AGTTCCAAAA GCGACTTCAG
    GAATCAAAAC AAAAGGATGA AGATGATGAT GAAGAAGAGG ACGATGATGT GGATACTATG
    CTTATCATGC AGCGGCTGGA GGCAGAAAGA AGAGCAAGGG ATGAAGAACG CAGACGACAG
    CAACAGTTAG AAGAAATGCG CAAGCGAGAA GCAGAAGACA GGGCCAGGCA GGAAGAGGAG
    CGCCGACAGC AAGAGGAGGA GCGAACCAGG AGAGAGGCAG AAGAAAAGAG GCGACAGGAA
    GAGGAGTATT ACAATCGCTT AGAGGCTGAA AGGCGCAGGC AGCACGATGA AGCAGAACGA
    AGATTGCTGG AACCAGACGA GCCTGGTCTG TACAGACCTC CACTTCCACG GGAATATGAA
    CTCCCATCCC CAACCCCTTC ATCTAACGCT CCTCCTCCCC CACCTCAGCG AAACACCTCT
    TACCTCAAAA CACAGGTCCT CTCCCCTGAC ACGATTTATA CTGCCAAGTT TGTTGCATAC
    AATGATGATG AGGAGGAGGA GGATTCCAAT CTAGCAGGTC AGGATAAGTA CTCCAGCACA
    AGAAAATCTT ATGGTGACTT TCCTCCTGCC ACCCTTAAAC CACAGCAGCC CTCCCGCCAG
    CCTCGCCCAG TTTCAGATGG CCTCTTTCTT TCCAACTCAT TCCAGTCATC TCCAGTCAAT
    GCCAACAGTA CTGCTGCCAA AAAAAGCCAG CCCCCGCCCC CACCAAACAA ACCGAGTTTC
    ATCCATGCCA GCAGCTCTAA AGGAATAAAT TCATACACTG GATCTTCGGG AGCAACGGTT
    GGGGCCCATG AAGTCTATCG GGATCCAAGA GACAAACGAT CTAAAAGTCA AGATACAGAT
    TTACCTGGAG CACCAGAAAA TCTGACGTTC AGGGAACGCC AGCGACTTTT TTCACAGGGT
    CAAGATGTAT CCAATAAAGT AAAAGCCTCT AGGAAACTAA TGGAACTGGA AAATGAGTTG
    AACACGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33804
    Clone ID Related Accession (Same CDS sequence) XM_015284354.2 , XM_015284354.1
    Accession Version XM_015284354.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGTCTGTACA GACCTCCACT TCCACGGGAA
    TATGAACTCC CATCCCCAAC CCCTTCATCT AACGCTCCTC CTCCCCCACC TCAGCGAAAC
    ACCTCTTACC TCAAAACACA GGTCCTCTCC CCTGACACGA TTTATACTGC CAAGTTTGTT
    GCATACAATG ATGATGAGGA GGAGGAGGAT TCCAATCTAG CAGGAATAAA TTCATACACT
    GGATCTTCGG GAGCAACGGT TGGGGCCCAT GAAGTCTATC GGGATCCAAG AGACAAACGA
    TCTAAAAGTC AAGATACAGA TTTACCTGGA GCACCAGAAA ATCTGACGTT CAGGGAACGC
    CAGCGACTTT TTTCACAGGG TCAAGATGTA TCCAATAAAG TAAAAGCCTC TAGGAAACTA
    ATGGAACTGG AAAATGAGTT GAACACGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139840.1
    CDS2885..8437
    Translation

    Target ORF information:

    RefSeq Version XM_015284354.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284354.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGTCTGTACA GACCTCCACT TCCACGGGAA
    TATGAACTCC CATCCCCAAC CCCTTCATCT AACGCTCCTC CTCCCCCACC TCAGCGAAAC
    ACCTCTTACC TCAAAACACA GGTCCTCTCC CCTGACACGA TTTATACTGC CAAGTTTGTT
    GCATACAATG ATGATGAGGA GGAGGAGGAT TCCAATCTAG CAGGAATAAA TTCATACACT
    GGATCTTCGG GAGCAACGGT TGGGGCCCAT GAAGTCTATC GGGATCCAAG AGACAAACGA
    TCTAAAAGTC AAGATACAGA TTTACCTGGA GCACCAGAAA ATCTGACGTT CAGGGAACGC
    CAGCGACTTT TTTCACAGGG TCAAGATGTA TCCAATAAAG TAAAAGCCTC TAGGAAACTA
    ATGGAACTGG AAAATGAGTT GAACACGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33804
    Clone ID Related Accession (Same CDS sequence) XM_015284354.2 , XM_015284354.1
    Accession Version XM_015284354.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGTCTGTACA GACCTCCACT TCCACGGGAA
    TATGAACTCC CATCCCCAAC CCCTTCATCT AACGCTCCTC CTCCCCCACC TCAGCGAAAC
    ACCTCTTACC TCAAAACACA GGTCCTCTCC CCTGACACGA TTTATACTGC CAAGTTTGTT
    GCATACAATG ATGATGAGGA GGAGGAGGAT TCCAATCTAG CAGGAATAAA TTCATACACT
    GGATCTTCGG GAGCAACGGT TGGGGCCCAT GAAGTCTATC GGGATCCAAG AGACAAACGA
    TCTAAAAGTC AAGATACAGA TTTACCTGGA GCACCAGAAA ATCTGACGTT CAGGGAACGC
    CAGCGACTTT TTTCACAGGG TCAAGATGTA TCCAATAAAG TAAAAGCCTC TAGGAAACTA
    ATGGAACTGG AAAATGAGTT GAACACGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139840.1
    CDS33..5585
    Translation

    Target ORF information:

    RefSeq Version XM_015284354.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284354.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGTCTGTACA GACCTCCACT TCCACGGGAA
    TATGAACTCC CATCCCCAAC CCCTTCATCT AACGCTCCTC CTCCCCCACC TCAGCGAAAC
    ACCTCTTACC TCAAAACACA GGTCCTCTCC CCTGACACGA TTTATACTGC CAAGTTTGTT
    GCATACAATG ATGATGAGGA GGAGGAGGAT TCCAATCTAG CAGGAATAAA TTCATACACT
    GGATCTTCGG GAGCAACGGT TGGGGCCCAT GAAGTCTATC GGGATCCAAG AGACAAACGA
    TCTAAAAGTC AAGATACAGA TTTACCTGGA GCACCAGAAA ATCTGACGTT CAGGGAACGC
    CAGCGACTTT TTTCACAGGG TCAAGATGTA TCCAATAAAG TAAAAGCCTC TAGGAAACTA
    ATGGAACTGG AAAATGAGTT GAACACGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33805
    Clone ID Related Accession (Same CDS sequence) XM_015284355.1 , XM_015284355.2
    Accession Version XM_015284355.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAAGAA GATTAGATGT GGATGAGAAG CCTCTTGTTG TACAATTAAA CTGGAACAAA
    GATGATCGAG AAGGAAGGTT TGTTCTCAAG AATGAAAATG ATACGCTTCC TCCAAAGAAG
    GCTCAGAGTA ATGGCCCTGA AAAACAAGAA AAAGAAGGAG TAATACAGAA TTTCAAACGA
    ACTCTCTCAA AGAAAGAAAA GAAAGAGAAG AAGAGGCGTG AAAAAGAGGC ATTGCGACAA
    GCATCAGATA AAGATGATCG ACTTCTTCAT GGGGATGATA TTGAGAATTC TCGCCTTGCA
    GCTGAGGTTT ACAAAGATAT GCCTGAGACC AGCTTCACTC GCACAATATC TAATCCTGAG
    GTGGTTATGA AACGACGGCG GCAGCAAAAA CTAGAGAAGA GAATGCAGGA ATTTCGCAGT
    TCTGATGGCA GACCAGACTC AGGTGGGACG CTGAGAATTT ATGCGGACAG TTTAAAACCA
    AATATACCAT ACAAGACAAT CCTGTTGTCA ACTACAGATA CTGCAGACTT TGCAGTTATT
    GAAGCACTGG AGAAATATGG CTTGGAGAAG GAAAACCCCA AGGATTATTG TATTGCTCGT
    GTCATTCTGC CTCCTGGTGC TCAGCACTCT GATGATAAAG GTGCTAAAGA AACTATTCTA
    GATGATGATG AGTGTCCACT TCAGATATTC AGGGAATGGC CTAGTGATAA AGGAATACTA
    GTCTTTCAGT TGAAAAGGCG GCCTCCTGAT TATGTCCCAA AGAAATCCAA GAAAATAGCT
    GATGGAAAGC CATTAAAGGG GAAGGAAAGA GTTGACGGGT CTGGCTACGG CTCTTGCCTT
    CCTCCTGAGA AACTACCATA CCTGGTAGAA TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACGCTTA TGAAGATGGT TCTGACTCCA GAGATAAACC AAAGCTTTAT
    CGTCTACAAT TAAGTGTCAC TGAAGTTGGA ACTGAAAAAT TTGATGACAA TTCCATTCAG
    TTATTTGGTC CAGGAATTCA GCCTCATCAT TGTGATCTTA CTAATATGGA TGGAGTTGTT
    ACTGTAACCC CAAGAAACAT TGATGCGGAA ACGTATGTAG AAGGTCAGCG TATTTCAGAA
    ACCACTATGC TGCAAAGTGG AATGAAGGTT CAGTTTGGGT CATCTCATGT GTTTAAGTTT
    GTGGATCCCA CTCAGGACCA TATTATTACT AAAAGACCTA TTGATGCAAG CCTTATGGTC
    AAAGGTCCAA GGCATAAAGC TGGAGCTGTT CAGGAAACCA CGTTTGATCT GGAGGGAGAT
    GTTCACAGTG GAACAGCGTT ACCAGCGAGC AAGAGCACCA GCAGGCTTGA TGGTGACAGA
    ACATCATCAG CATCCAGCAC AGCTGAACGA GGAATGGTGA AGCCCATGAT TAGAATAGAA
    CAGCAGCAAG ATTATCGCAG ACAGGAAAGC AGACCTCAGG ATACCCATGG ACCAGAACTG
    ATCCTACCTG CCAGTATTGA ATTCAGGGAA AGCTCTGAAG ATGCCTTCTT ATCTGCCATT
    ATAAATTATA CAAATAGCTC GACAGTTCAT TTTAAACTGT CACCCACGTA TGTTTTGTAT
    ATGACATGTC GGTATGTATT GTCAAGCCAG TACAGACCTG ACATCACTCC TACTGAGCGT
    ACTCACAAAG TTATTGCAAT AGTCAACAAG ATGGTGAATA TGATGGAAGG AGTCATACAG
    AAACAGAAGA ATATTGCTGG GGCACTTGCC TTTTGGATGG CAAATGCGTC TGAATTGCTG
    AATTTTATAA AGCAAGACCG TGATCTGAGC CGAATCACTG TGGATGCTCA AGATGTTTTG
    GCGCACTTGG TTCAAATGGC ATTCAAGTAC CTAGTTCATT GTCTGCAGTC AGAGCTTAAT
    AACTACATGC CAGCATTTCT TGATGATCCA GAGGAAAATA ATCCACAGAG GCCTAAAATA
    GATGATGTGC TGCACACATT GACTGGAGCT ATGTCCTTGT TGCGACGATG TAGAGTGAAT
    GCTGCACTGA CTATACAGCT CTTCTCCCAG TTGTTTCATT TTATCAACAT GTGGCTTTTT
    AACAGACTAG TTACGGCTCC AGATTCAGGG TTATGTTCCC ACTACTGGGG AGCTATTGTT
    CGTCAGCAGT TGGGTCATAT TGAGGCCTGG GCAGAAAAAC AGGGTTTAGA ATTGGCTGCT
    GATTGCCACT TAAGCAGAAT AGTGCAGGCA ACCACATTGC TCACCATGGA CAAGTACTCA
    CATGCTGATG TTCCAAATAT TAACAGTACT TGCTTTAAGT TGAATTCTCT ACAGCTACAA
    GCTTTGTTGC AGAATTATCA CTGTGCTCCA GATGAACCGC TTATACCAAC AGAACTGATA
    GAGAATGTAG TAACTGTTGC AGAAAATACG GCTGATGAAC TTGCTCGCAG TGATGGCAGA
    GAAGTCCAAT TGGAAGAGGA TCCTGACTTA CAACTCCCTT TTCTTTTACC AGAAGATGGC
    TATTCCTGTG ATGTTGTCAG AAATGTTCCA AGTGGTTTAC AAGAATTTTT GGATCCCCTC
    TGTCAGAGAG GTTTTTGTAG GTTAATACCT CATACACGGT CACCAGGCAC ATGGACAATA
    TACTTTGAAG GCGCAGACTA TGAAAGCCAC CTGTCACATG AGAATGCTGA GCTAGCTCAG
    CCTTTGAGGA AAGAACCTGA GATTATAACT GTAACACTAA AAAAACAAAA TGGAATGGGC
    CTCAGCATTG TTGCTGCCAA GGGTGCTGGT CAAGACAAAC TTGGAATATA TGTCAAGTCT
    GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGTCGTCTGG CAGCAGGGGA TCAGCTGCTC
    AGTGTGGATG GTCGAAGCTT GGTGGGGCTG TCCCAGGAAA GGGCAGCAGA ACTTATGACG
    AGGACAGGTT CTGTTGTAAC ACTGGAAGTA GCAAAACAAG GAGCAATATA CCACGGTCTG
    GCAACCCTGC TAAATCAGCC ATCTCCAATG ATGCAAAGAG TTTCTGACCG CCGTGGCTCA
    GGTAAACCCC GACCAAAGAG TGAAGGTTTT GAGCTGTATA ACAACTCCAC GCAAAATGGG
    TCACCTGAGA GCCCCCAGCT GCCATGGACG GAGTACAGTG AACCAAAGAA GCTGCCAGGA
    GATGACAGAT TAATGAAGAA CAGAGCCGAT CATCGCTCCA GCCCCAACGT AGCAAATCAG
    CCTCCAAGCC CTGGGGGGAA GAATGCTTAT GCATCTGGAA CTACCACCAA AATAACCTCA
    GTCTCGACTG GAAATCTTTG TACAGAGGAG CAAACTCTGC CTCCACGACC TGAAGCTTAT
    CCTATCCCAA CACAGACCTA TACCAGAGAA TATTTCACAT TCCCAGCTTC AAAATCCCAG
    GATCGAATGG GTCCAGCTCA GAGTCAGTGG CCAAATTATG AAGAGAAGCC ACAGATCCAG
    GCAGAAAGTA ATCACTCAAT CAATACTACA ATGCAGCGCG TGGCTCGATC CCAGGAAGAA
    CTCCGAGATA AAGTTTTCCA GCCAGAGAGG AATCGTTTGG AAGTTGTTAT CCGGAAGGAC
    ATAGAATACA TTGATCGGAA GTCAGACAGC GATCAGTGGA TGAATTCATC TGTGGATTCT
    AGCACATCTA GCCAAGAGCA CCTGAACCAT ACATCCAAAC CTGTTTCACC TGGTTCTTGC
    TTAACTAAAA GTGGACCTGG CCGTTGGAAA ACTCCAGCTA CGAGCCAGCC AACTCCAGTG
    GCCGTTTCCC AGCCCATCAG AACAGACCTT CCTCCTCCGC CGCCACCTCC TCCGGTGCAT
    TATGCAGCTG AATTTGATGA ACCACAAATG GATTTGCCTC TGCCTCCCCC TCCTCCACCG
    AACCAAGTAG GAGCACAAGC ATCTTCTCAG GTGGCTGCTG CTGAGCGTAA AAAACGAGAA
    GAGCATCAGA GGTGGTATGA AAAGGAAAAG GCACGTTTGG AAGAAGAACG AGAAAGGAAA
    CGCCGTGAGC AAGAGAGAAA ACTGGGCCAA ATGCGTTCGC AGCCACTAAT TCCTCCCCAG
    CCTGTGGTTC CTCCTGTTTC CCTTCAGCAA GTGAAATCGG AAAAAACTTC AACTTTCCAG
    AGGTCTCAAG AAACAGTCAT TCGAGAGTTG CAGCCGCAAC AGCAGCCTCG GACTATTGAG
    CGGAGAGATC TGCAGTACAT TACTATCAGC AAGGAAGAAT TGTCCTCTAG TGACAGCCTC
    TCTCCAGATC CCTGGAAACG AGATGCCAAG GAGAAACTGG AGAAGCAGCA GCAGATGCAC
    ATTGTGGACA TGCTGAGCAA AGAGATTCAG GATCTTCAGA ACAAGCCTGA TCGTACAGCT
    GAAGAGAACG ACCGCCTGCG CAAACTCATG CTAGAGTGGC AGTTCCAAAA GCGACTTCAG
    GAATCAAAAC AAAAGGATGA AGATGATGAT GAAGAAGAGG ACGATGATGT GGATACTATG
    CTTATCATGC AGCGGCTGGA GGCAGAAAGA AGAGCAAGGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGTCTGTACA GACCTCCACT TCCACGGGAA
    TATGAACTCC CATCCCCAAC CCCTTCATCT AACGCTCCTC CTCCCCCACC TCAGCGAAAC
    ACCTCTTACC TCAAAACACA GGTCCTCTCC CCTGACACGA TTTATACTGC CAAGTTTGTT
    GCATACAATG ATGATGAGGA GGAGGAGGAT TCCAATCTAG CAGGAATAAA TTCATACACT
    GGATCTTCGG GAGCAACGGT TGGGGCCCAT GAAGTCTATC GGGATCCAAG AGACAAACGA
    TCTAAAAGTC AAGATACAGA TTTACCTGGA GCACCAGAAA ATCTGACGTT CAGGGAACGC
    CAGCGACTTT TTTCACAGGG TCAAGATGTA TCCAATAAAG TAAAAGCCTC TAGGAAACTA
    ATGGAACTGG AAAATGAGTT GAACACGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139841.1
    CDS69..5561
    Translation

    Target ORF information:

    RefSeq Version XM_015284355.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284355.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAAGAA GATTAGATGT GGATGAGAAG CCTCTTGTTG TACAATTAAA CTGGAACAAA
    GATGATCGAG AAGGAAGGTT TGTTCTCAAG AATGAAAATG ATACGCTTCC TCCAAAGAAG
    GCTCAGAGTA ATGGCCCTGA AAAACAAGAA AAAGAAGGAG TAATACAGAA TTTCAAACGA
    ACTCTCTCAA AGAAAGAAAA GAAAGAGAAG AAGAGGCGTG AAAAAGAGGC ATTGCGACAA
    GCATCAGATA AAGATGATCG ACTTCTTCAT GGGGATGATA TTGAGAATTC TCGCCTTGCA
    GCTGAGGTTT ACAAAGATAT GCCTGAGACC AGCTTCACTC GCACAATATC TAATCCTGAG
    GTGGTTATGA AACGACGGCG GCAGCAAAAA CTAGAGAAGA GAATGCAGGA ATTTCGCAGT
    TCTGATGGCA GACCAGACTC AGGTGGGACG CTGAGAATTT ATGCGGACAG TTTAAAACCA
    AATATACCAT ACAAGACAAT CCTGTTGTCA ACTACAGATA CTGCAGACTT TGCAGTTATT
    GAAGCACTGG AGAAATATGG CTTGGAGAAG GAAAACCCCA AGGATTATTG TATTGCTCGT
    GTCATTCTGC CTCCTGGTGC TCAGCACTCT GATGATAAAG GTGCTAAAGA AACTATTCTA
    GATGATGATG AGTGTCCACT TCAGATATTC AGGGAATGGC CTAGTGATAA AGGAATACTA
    GTCTTTCAGT TGAAAAGGCG GCCTCCTGAT TATGTCCCAA AGAAATCCAA GAAAATAGCT
    GATGGAAAGC CATTAAAGGG GAAGGAAAGA GTTGACGGGT CTGGCTACGG CTCTTGCCTT
    CCTCCTGAGA AACTACCATA CCTGGTAGAA TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACGCTTA TGAAGATGGT TCTGACTCCA GAGATAAACC AAAGCTTTAT
    CGTCTACAAT TAAGTGTCAC TGAAGTTGGA ACTGAAAAAT TTGATGACAA TTCCATTCAG
    TTATTTGGTC CAGGAATTCA GCCTCATCAT TGTGATCTTA CTAATATGGA TGGAGTTGTT
    ACTGTAACCC CAAGAAACAT TGATGCGGAA ACGTATGTAG AAGGTCAGCG TATTTCAGAA
    ACCACTATGC TGCAAAGTGG AATGAAGGTT CAGTTTGGGT CATCTCATGT GTTTAAGTTT
    GTGGATCCCA CTCAGGACCA TATTATTACT AAAAGACCTA TTGATGCAAG CCTTATGGTC
    AAAGGTCCAA GGCATAAAGC TGGAGCTGTT CAGGAAACCA CGTTTGATCT GGAGGGAGAT
    GTTCACAGTG GAACAGCGTT ACCAGCGAGC AAGAGCACCA GCAGGCTTGA TGGTGACAGA
    ACATCATCAG CATCCAGCAC AGCTGAACGA GGAATGGTGA AGCCCATGAT TAGAATAGAA
    CAGCAGCAAG ATTATCGCAG ACAGGAAAGC AGACCTCAGG ATACCCATGG ACCAGAACTG
    ATCCTACCTG CCAGTATTGA ATTCAGGGAA AGCTCTGAAG ATGCCTTCTT ATCTGCCATT
    ATAAATTATA CAAATAGCTC GACAGTTCAT TTTAAACTGT CACCCACGTA TGTTTTGTAT
    ATGACATGTC GGTATGTATT GTCAAGCCAG TACAGACCTG ACATCACTCC TACTGAGCGT
    ACTCACAAAG TTATTGCAAT AGTCAACAAG ATGGTGAATA TGATGGAAGG AGTCATACAG
    AAACAGAAGA ATATTGCTGG GGCACTTGCC TTTTGGATGG CAAATGCGTC TGAATTGCTG
    AATTTTATAA AGCAAGACCG TGATCTGAGC CGAATCACTG TGGATGCTCA AGATGTTTTG
    GCGCACTTGG TTCAAATGGC ATTCAAGTAC CTAGTTCATT GTCTGCAGTC AGAGCTTAAT
    AACTACATGC CAGCATTTCT TGATGATCCA GAGGAAAATA ATCCACAGAG GCCTAAAATA
    GATGATGTGC TGCACACATT GACTGGAGCT ATGTCCTTGT TGCGACGATG TAGAGTGAAT
    GCTGCACTGA CTATACAGCT CTTCTCCCAG TTGTTTCATT TTATCAACAT GTGGCTTTTT
    AACAGACTAG TTACGGCTCC AGATTCAGGG TTATGTTCCC ACTACTGGGG AGCTATTGTT
    CGTCAGCAGT TGGGTCATAT TGAGGCCTGG GCAGAAAAAC AGGGTTTAGA ATTGGCTGCT
    GATTGCCACT TAAGCAGAAT AGTGCAGGCA ACCACATTGC TCACCATGGA CAAGTACTCA
    CATGCTGATG TTCCAAATAT TAACAGTACT TGCTTTAAGT TGAATTCTCT ACAGCTACAA
    GCTTTGTTGC AGAATTATCA CTGTGCTCCA GATGAACCGC TTATACCAAC AGAACTGATA
    GAGAATGTAG TAACTGTTGC AGAAAATACG GCTGATGAAC TTGCTCGCAG TGATGGCAGA
    GAAGTCCAAT TGGAAGAGGA TCCTGACTTA CAACTCCCTT TTCTTTTACC AGAAGATGGC
    TATTCCTGTG ATGTTGTCAG AAATGTTCCA AGTGGTTTAC AAGAATTTTT GGATCCCCTC
    TGTCAGAGAG GTTTTTGTAG GTTAATACCT CATACACGGT CACCAGGCAC ATGGACAATA
    TACTTTGAAG GCGCAGACTA TGAAAGCCAC CTGTCACATG AGAATGCTGA GCTAGCTCAG
    CCTTTGAGGA AAGAACCTGA GATTATAACT GTAACACTAA AAAAACAAAA TGGAATGGGC
    CTCAGCATTG TTGCTGCCAA GGGTGCTGGT CAAGACAAAC TTGGAATATA TGTCAAGTCT
    GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGTCGTCTGG CAGCAGGGGA TCAGCTGCTC
    AGTGTGGATG GTCGAAGCTT GGTGGGGCTG TCCCAGGAAA GGGCAGCAGA ACTTATGACG
    AGGACAGGTT CTGTTGTAAC ACTGGAAGTA GCAAAACAAG GAGCAATATA CCACGGTCTG
    GCAACCCTGC TAAATCAGCC ATCTCCAATG ATGCAAAGAG TTTCTGACCG CCGTGGCTCA
    GGTAAACCCC GACCAAAGAG TGAAGGTTTT GAGCTGTATA ACAACTCCAC GCAAAATGGG
    TCACCTGAGA GCCCCCAGCT GCCATGGACG GAGTACAGTG AACCAAAGAA GCTGCCAGGA
    GATGACAGAT TAATGAAGAA CAGAGCCGAT CATCGCTCCA GCCCCAACGT AGCAAATCAG
    CCTCCAAGCC CTGGGGGGAA GAATGCTTAT GCATCTGGAA CTACCACCAA AATAACCTCA
    GTCTCGACTG GAAATCTTTG TACAGAGGAG CAAACTCTGC CTCCACGACC TGAAGCTTAT
    CCTATCCCAA CACAGACCTA TACCAGAGAA TATTTCACAT TCCCAGCTTC AAAATCCCAG
    GATCGAATGG GTCCAGCTCA GAGTCAGTGG CCAAATTATG AAGAGAAGCC ACAGATCCAG
    GCAGAAAGTA ATCACTCAAT CAATACTACA ATGCAGCGCG TGGCTCGATC CCAGGAAGAA
    CTCCGAGATA AAGTTTTCCA GCCAGAGAGG AATCGTTTGG AAGTTGTTAT CCGGAAGGAC
    ATAGAATACA TTGATCGGAA GTCAGACAGC GATCAGTGGA TGAATTCATC TGTGGATTCT
    AGCACATCTA GCCAAGAGCA CCTGAACCAT ACATCCAAAC CTGTTTCACC TGGTTCTTGC
    TTAACTAAAA GTGGACCTGG CCGTTGGAAA ACTCCAGCTA CGAGCCAGCC AACTCCAGTG
    GCCGTTTCCC AGCCCATCAG AACAGACCTT CCTCCTCCGC CGCCACCTCC TCCGGTGCAT
    TATGCAGCTG AATTTGATGA ACCACAAATG GATTTGCCTC TGCCTCCCCC TCCTCCACCG
    AACCAAGTAG GAGCACAAGC ATCTTCTCAG GTGGCTGCTG CTGAGCGTAA AAAACGAGAA
    GAGCATCAGA GGTGGTATGA AAAGGAAAAG GCACGTTTGG AAGAAGAACG AGAAAGGAAA
    CGCCGTGAGC AAGAGAGAAA ACTGGGCCAA ATGCGTTCGC AGCCACTAAT TCCTCCCCAG
    CCTGTGGTTC CTCCTGTTTC CCTTCAGCAA GTGAAATCGG AAAAAACTTC AACTTTCCAG
    AGGTCTCAAG AAACAGTCAT TCGAGAGTTG CAGCCGCAAC AGCAGCCTCG GACTATTGAG
    CGGAGAGATC TGCAGTACAT TACTATCAGC AAGGAAGAAT TGTCCTCTAG TGACAGCCTC
    TCTCCAGATC CCTGGAAACG AGATGCCAAG GAGAAACTGG AGAAGCAGCA GCAGATGCAC
    ATTGTGGACA TGCTGAGCAA AGAGATTCAG GATCTTCAGA ACAAGCCTGA TCGTACAGCT
    GAAGAGAACG ACCGCCTGCG CAAACTCATG CTAGAGTGGC AGTTCCAAAA GCGACTTCAG
    GAATCAAAAC AAAAGGATGA AGATGATGAT GAAGAAGAGG ACGATGATGT GGATACTATG
    CTTATCATGC AGCGGCTGGA GGCAGAAAGA AGAGCAAGGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGTCTGTACA GACCTCCACT TCCACGGGAA
    TATGAACTCC CATCCCCAAC CCCTTCATCT AACGCTCCTC CTCCCCCACC TCAGCGAAAC
    ACCTCTTACC TCAAAACACA GGTCCTCTCC CCTGACACGA TTTATACTGC CAAGTTTGTT
    GCATACAATG ATGATGAGGA GGAGGAGGAT TCCAATCTAG CAGGAATAAA TTCATACACT
    GGATCTTCGG GAGCAACGGT TGGGGCCCAT GAAGTCTATC GGGATCCAAG AGACAAACGA
    TCTAAAAGTC AAGATACAGA TTTACCTGGA GCACCAGAAA ATCTGACGTT CAGGGAACGC
    CAGCGACTTT TTTCACAGGG TCAAGATGTA TCCAATAAAG TAAAAGCCTC TAGGAAACTA
    ATGGAACTGG AAAATGAGTT GAACACGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33805
    Clone ID Related Accession (Same CDS sequence) XM_015284355.1 , XM_015284355.2
    Accession Version XM_015284355.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAAGAA GATTAGATGT GGATGAGAAG CCTCTTGTTG TACAATTAAA CTGGAACAAA
    GATGATCGAG AAGGAAGGTT TGTTCTCAAG AATGAAAATG ATACGCTTCC TCCAAAGAAG
    GCTCAGAGTA ATGGCCCTGA AAAACAAGAA AAAGAAGGAG TAATACAGAA TTTCAAACGA
    ACTCTCTCAA AGAAAGAAAA GAAAGAGAAG AAGAGGCGTG AAAAAGAGGC ATTGCGACAA
    GCATCAGATA AAGATGATCG ACTTCTTCAT GGGGATGATA TTGAGAATTC TCGCCTTGCA
    GCTGAGGTTT ACAAAGATAT GCCTGAGACC AGCTTCACTC GCACAATATC TAATCCTGAG
    GTGGTTATGA AACGACGGCG GCAGCAAAAA CTAGAGAAGA GAATGCAGGA ATTTCGCAGT
    TCTGATGGCA GACCAGACTC AGGTGGGACG CTGAGAATTT ATGCGGACAG TTTAAAACCA
    AATATACCAT ACAAGACAAT CCTGTTGTCA ACTACAGATA CTGCAGACTT TGCAGTTATT
    GAAGCACTGG AGAAATATGG CTTGGAGAAG GAAAACCCCA AGGATTATTG TATTGCTCGT
    GTCATTCTGC CTCCTGGTGC TCAGCACTCT GATGATAAAG GTGCTAAAGA AACTATTCTA
    GATGATGATG AGTGTCCACT TCAGATATTC AGGGAATGGC CTAGTGATAA AGGAATACTA
    GTCTTTCAGT TGAAAAGGCG GCCTCCTGAT TATGTCCCAA AGAAATCCAA GAAAATAGCT
    GATGGAAAGC CATTAAAGGG GAAGGAAAGA GTTGACGGGT CTGGCTACGG CTCTTGCCTT
    CCTCCTGAGA AACTACCATA CCTGGTAGAA TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACGCTTA TGAAGATGGT TCTGACTCCA GAGATAAACC AAAGCTTTAT
    CGTCTACAAT TAAGTGTCAC TGAAGTTGGA ACTGAAAAAT TTGATGACAA TTCCATTCAG
    TTATTTGGTC CAGGAATTCA GCCTCATCAT TGTGATCTTA CTAATATGGA TGGAGTTGTT
    ACTGTAACCC CAAGAAACAT TGATGCGGAA ACGTATGTAG AAGGTCAGCG TATTTCAGAA
    ACCACTATGC TGCAAAGTGG AATGAAGGTT CAGTTTGGGT CATCTCATGT GTTTAAGTTT
    GTGGATCCCA CTCAGGACCA TATTATTACT AAAAGACCTA TTGATGCAAG CCTTATGGTC
    AAAGGTCCAA GGCATAAAGC TGGAGCTGTT CAGGAAACCA CGTTTGATCT GGAGGGAGAT
    GTTCACAGTG GAACAGCGTT ACCAGCGAGC AAGAGCACCA GCAGGCTTGA TGGTGACAGA
    ACATCATCAG CATCCAGCAC AGCTGAACGA GGAATGGTGA AGCCCATGAT TAGAATAGAA
    CAGCAGCAAG ATTATCGCAG ACAGGAAAGC AGACCTCAGG ATACCCATGG ACCAGAACTG
    ATCCTACCTG CCAGTATTGA ATTCAGGGAA AGCTCTGAAG ATGCCTTCTT ATCTGCCATT
    ATAAATTATA CAAATAGCTC GACAGTTCAT TTTAAACTGT CACCCACGTA TGTTTTGTAT
    ATGACATGTC GGTATGTATT GTCAAGCCAG TACAGACCTG ACATCACTCC TACTGAGCGT
    ACTCACAAAG TTATTGCAAT AGTCAACAAG ATGGTGAATA TGATGGAAGG AGTCATACAG
    AAACAGAAGA ATATTGCTGG GGCACTTGCC TTTTGGATGG CAAATGCGTC TGAATTGCTG
    AATTTTATAA AGCAAGACCG TGATCTGAGC CGAATCACTG TGGATGCTCA AGATGTTTTG
    GCGCACTTGG TTCAAATGGC ATTCAAGTAC CTAGTTCATT GTCTGCAGTC AGAGCTTAAT
    AACTACATGC CAGCATTTCT TGATGATCCA GAGGAAAATA ATCCACAGAG GCCTAAAATA
    GATGATGTGC TGCACACATT GACTGGAGCT ATGTCCTTGT TGCGACGATG TAGAGTGAAT
    GCTGCACTGA CTATACAGCT CTTCTCCCAG TTGTTTCATT TTATCAACAT GTGGCTTTTT
    AACAGACTAG TTACGGCTCC AGATTCAGGG TTATGTTCCC ACTACTGGGG AGCTATTGTT
    CGTCAGCAGT TGGGTCATAT TGAGGCCTGG GCAGAAAAAC AGGGTTTAGA ATTGGCTGCT
    GATTGCCACT TAAGCAGAAT AGTGCAGGCA ACCACATTGC TCACCATGGA CAAGTACTCA
    CATGCTGATG TTCCAAATAT TAACAGTACT TGCTTTAAGT TGAATTCTCT ACAGCTACAA
    GCTTTGTTGC AGAATTATCA CTGTGCTCCA GATGAACCGC TTATACCAAC AGAACTGATA
    GAGAATGTAG TAACTGTTGC AGAAAATACG GCTGATGAAC TTGCTCGCAG TGATGGCAGA
    GAAGTCCAAT TGGAAGAGGA TCCTGACTTA CAACTCCCTT TTCTTTTACC AGAAGATGGC
    TATTCCTGTG ATGTTGTCAG AAATGTTCCA AGTGGTTTAC AAGAATTTTT GGATCCCCTC
    TGTCAGAGAG GTTTTTGTAG GTTAATACCT CATACACGGT CACCAGGCAC ATGGACAATA
    TACTTTGAAG GCGCAGACTA TGAAAGCCAC CTGTCACATG AGAATGCTGA GCTAGCTCAG
    CCTTTGAGGA AAGAACCTGA GATTATAACT GTAACACTAA AAAAACAAAA TGGAATGGGC
    CTCAGCATTG TTGCTGCCAA GGGTGCTGGT CAAGACAAAC TTGGAATATA TGTCAAGTCT
    GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGTCGTCTGG CAGCAGGGGA TCAGCTGCTC
    AGTGTGGATG GTCGAAGCTT GGTGGGGCTG TCCCAGGAAA GGGCAGCAGA ACTTATGACG
    AGGACAGGTT CTGTTGTAAC ACTGGAAGTA GCAAAACAAG GAGCAATATA CCACGGTCTG
    GCAACCCTGC TAAATCAGCC ATCTCCAATG ATGCAAAGAG TTTCTGACCG CCGTGGCTCA
    GGTAAACCCC GACCAAAGAG TGAAGGTTTT GAGCTGTATA ACAACTCCAC GCAAAATGGG
    TCACCTGAGA GCCCCCAGCT GCCATGGACG GAGTACAGTG AACCAAAGAA GCTGCCAGGA
    GATGACAGAT TAATGAAGAA CAGAGCCGAT CATCGCTCCA GCCCCAACGT AGCAAATCAG
    CCTCCAAGCC CTGGGGGGAA GAATGCTTAT GCATCTGGAA CTACCACCAA AATAACCTCA
    GTCTCGACTG GAAATCTTTG TACAGAGGAG CAAACTCTGC CTCCACGACC TGAAGCTTAT
    CCTATCCCAA CACAGACCTA TACCAGAGAA TATTTCACAT TCCCAGCTTC AAAATCCCAG
    GATCGAATGG GTCCAGCTCA GAGTCAGTGG CCAAATTATG AAGAGAAGCC ACAGATCCAG
    GCAGAAAGTA ATCACTCAAT CAATACTACA ATGCAGCGCG TGGCTCGATC CCAGGAAGAA
    CTCCGAGATA AAGTTTTCCA GCCAGAGAGG AATCGTTTGG AAGTTGTTAT CCGGAAGGAC
    ATAGAATACA TTGATCGGAA GTCAGACAGC GATCAGTGGA TGAATTCATC TGTGGATTCT
    AGCACATCTA GCCAAGAGCA CCTGAACCAT ACATCCAAAC CTGTTTCACC TGGTTCTTGC
    TTAACTAAAA GTGGACCTGG CCGTTGGAAA ACTCCAGCTA CGAGCCAGCC AACTCCAGTG
    GCCGTTTCCC AGCCCATCAG AACAGACCTT CCTCCTCCGC CGCCACCTCC TCCGGTGCAT
    TATGCAGCTG AATTTGATGA ACCACAAATG GATTTGCCTC TGCCTCCCCC TCCTCCACCG
    AACCAAGTAG GAGCACAAGC ATCTTCTCAG GTGGCTGCTG CTGAGCGTAA AAAACGAGAA
    GAGCATCAGA GGTGGTATGA AAAGGAAAAG GCACGTTTGG AAGAAGAACG AGAAAGGAAA
    CGCCGTGAGC AAGAGAGAAA ACTGGGCCAA ATGCGTTCGC AGCCACTAAT TCCTCCCCAG
    CCTGTGGTTC CTCCTGTTTC CCTTCAGCAA GTGAAATCGG AAAAAACTTC AACTTTCCAG
    AGGTCTCAAG AAACAGTCAT TCGAGAGTTG CAGCCGCAAC AGCAGCCTCG GACTATTGAG
    CGGAGAGATC TGCAGTACAT TACTATCAGC AAGGAAGAAT TGTCCTCTAG TGACAGCCTC
    TCTCCAGATC CCTGGAAACG AGATGCCAAG GAGAAACTGG AGAAGCAGCA GCAGATGCAC
    ATTGTGGACA TGCTGAGCAA AGAGATTCAG GATCTTCAGA ACAAGCCTGA TCGTACAGCT
    GAAGAGAACG ACCGCCTGCG CAAACTCATG CTAGAGTGGC AGTTCCAAAA GCGACTTCAG
    GAATCAAAAC AAAAGGATGA AGATGATGAT GAAGAAGAGG ACGATGATGT GGATACTATG
    CTTATCATGC AGCGGCTGGA GGCAGAAAGA AGAGCAAGGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGTCTGTACA GACCTCCACT TCCACGGGAA
    TATGAACTCC CATCCCCAAC CCCTTCATCT AACGCTCCTC CTCCCCCACC TCAGCGAAAC
    ACCTCTTACC TCAAAACACA GGTCCTCTCC CCTGACACGA TTTATACTGC CAAGTTTGTT
    GCATACAATG ATGATGAGGA GGAGGAGGAT TCCAATCTAG CAGGAATAAA TTCATACACT
    GGATCTTCGG GAGCAACGGT TGGGGCCCAT GAAGTCTATC GGGATCCAAG AGACAAACGA
    TCTAAAAGTC AAGATACAGA TTTACCTGGA GCACCAGAAA ATCTGACGTT CAGGGAACGC
    CAGCGACTTT TTTCACAGGG TCAAGATGTA TCCAATAAAG TAAAAGCCTC TAGGAAACTA
    ATGGAACTGG AAAATGAGTT GAACACGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139841.1
    CDS2885..8377
    Translation

    Target ORF information:

    RefSeq Version XM_015284355.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284355.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAAGAA GATTAGATGT GGATGAGAAG CCTCTTGTTG TACAATTAAA CTGGAACAAA
    GATGATCGAG AAGGAAGGTT TGTTCTCAAG AATGAAAATG ATACGCTTCC TCCAAAGAAG
    GCTCAGAGTA ATGGCCCTGA AAAACAAGAA AAAGAAGGAG TAATACAGAA TTTCAAACGA
    ACTCTCTCAA AGAAAGAAAA GAAAGAGAAG AAGAGGCGTG AAAAAGAGGC ATTGCGACAA
    GCATCAGATA AAGATGATCG ACTTCTTCAT GGGGATGATA TTGAGAATTC TCGCCTTGCA
    GCTGAGGTTT ACAAAGATAT GCCTGAGACC AGCTTCACTC GCACAATATC TAATCCTGAG
    GTGGTTATGA AACGACGGCG GCAGCAAAAA CTAGAGAAGA GAATGCAGGA ATTTCGCAGT
    TCTGATGGCA GACCAGACTC AGGTGGGACG CTGAGAATTT ATGCGGACAG TTTAAAACCA
    AATATACCAT ACAAGACAAT CCTGTTGTCA ACTACAGATA CTGCAGACTT TGCAGTTATT
    GAAGCACTGG AGAAATATGG CTTGGAGAAG GAAAACCCCA AGGATTATTG TATTGCTCGT
    GTCATTCTGC CTCCTGGTGC TCAGCACTCT GATGATAAAG GTGCTAAAGA AACTATTCTA
    GATGATGATG AGTGTCCACT TCAGATATTC AGGGAATGGC CTAGTGATAA AGGAATACTA
    GTCTTTCAGT TGAAAAGGCG GCCTCCTGAT TATGTCCCAA AGAAATCCAA GAAAATAGCT
    GATGGAAAGC CATTAAAGGG GAAGGAAAGA GTTGACGGGT CTGGCTACGG CTCTTGCCTT
    CCTCCTGAGA AACTACCATA CCTGGTAGAA TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACGCTTA TGAAGATGGT TCTGACTCCA GAGATAAACC AAAGCTTTAT
    CGTCTACAAT TAAGTGTCAC TGAAGTTGGA ACTGAAAAAT TTGATGACAA TTCCATTCAG
    TTATTTGGTC CAGGAATTCA GCCTCATCAT TGTGATCTTA CTAATATGGA TGGAGTTGTT
    ACTGTAACCC CAAGAAACAT TGATGCGGAA ACGTATGTAG AAGGTCAGCG TATTTCAGAA
    ACCACTATGC TGCAAAGTGG AATGAAGGTT CAGTTTGGGT CATCTCATGT GTTTAAGTTT
    GTGGATCCCA CTCAGGACCA TATTATTACT AAAAGACCTA TTGATGCAAG CCTTATGGTC
    AAAGGTCCAA GGCATAAAGC TGGAGCTGTT CAGGAAACCA CGTTTGATCT GGAGGGAGAT
    GTTCACAGTG GAACAGCGTT ACCAGCGAGC AAGAGCACCA GCAGGCTTGA TGGTGACAGA
    ACATCATCAG CATCCAGCAC AGCTGAACGA GGAATGGTGA AGCCCATGAT TAGAATAGAA
    CAGCAGCAAG ATTATCGCAG ACAGGAAAGC AGACCTCAGG ATACCCATGG ACCAGAACTG
    ATCCTACCTG CCAGTATTGA ATTCAGGGAA AGCTCTGAAG ATGCCTTCTT ATCTGCCATT
    ATAAATTATA CAAATAGCTC GACAGTTCAT TTTAAACTGT CACCCACGTA TGTTTTGTAT
    ATGACATGTC GGTATGTATT GTCAAGCCAG TACAGACCTG ACATCACTCC TACTGAGCGT
    ACTCACAAAG TTATTGCAAT AGTCAACAAG ATGGTGAATA TGATGGAAGG AGTCATACAG
    AAACAGAAGA ATATTGCTGG GGCACTTGCC TTTTGGATGG CAAATGCGTC TGAATTGCTG
    AATTTTATAA AGCAAGACCG TGATCTGAGC CGAATCACTG TGGATGCTCA AGATGTTTTG
    GCGCACTTGG TTCAAATGGC ATTCAAGTAC CTAGTTCATT GTCTGCAGTC AGAGCTTAAT
    AACTACATGC CAGCATTTCT TGATGATCCA GAGGAAAATA ATCCACAGAG GCCTAAAATA
    GATGATGTGC TGCACACATT GACTGGAGCT ATGTCCTTGT TGCGACGATG TAGAGTGAAT
    GCTGCACTGA CTATACAGCT CTTCTCCCAG TTGTTTCATT TTATCAACAT GTGGCTTTTT
    AACAGACTAG TTACGGCTCC AGATTCAGGG TTATGTTCCC ACTACTGGGG AGCTATTGTT
    CGTCAGCAGT TGGGTCATAT TGAGGCCTGG GCAGAAAAAC AGGGTTTAGA ATTGGCTGCT
    GATTGCCACT TAAGCAGAAT AGTGCAGGCA ACCACATTGC TCACCATGGA CAAGTACTCA
    CATGCTGATG TTCCAAATAT TAACAGTACT TGCTTTAAGT TGAATTCTCT ACAGCTACAA
    GCTTTGTTGC AGAATTATCA CTGTGCTCCA GATGAACCGC TTATACCAAC AGAACTGATA
    GAGAATGTAG TAACTGTTGC AGAAAATACG GCTGATGAAC TTGCTCGCAG TGATGGCAGA
    GAAGTCCAAT TGGAAGAGGA TCCTGACTTA CAACTCCCTT TTCTTTTACC AGAAGATGGC
    TATTCCTGTG ATGTTGTCAG AAATGTTCCA AGTGGTTTAC AAGAATTTTT GGATCCCCTC
    TGTCAGAGAG GTTTTTGTAG GTTAATACCT CATACACGGT CACCAGGCAC ATGGACAATA
    TACTTTGAAG GCGCAGACTA TGAAAGCCAC CTGTCACATG AGAATGCTGA GCTAGCTCAG
    CCTTTGAGGA AAGAACCTGA GATTATAACT GTAACACTAA AAAAACAAAA TGGAATGGGC
    CTCAGCATTG TTGCTGCCAA GGGTGCTGGT CAAGACAAAC TTGGAATATA TGTCAAGTCT
    GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGTCGTCTGG CAGCAGGGGA TCAGCTGCTC
    AGTGTGGATG GTCGAAGCTT GGTGGGGCTG TCCCAGGAAA GGGCAGCAGA ACTTATGACG
    AGGACAGGTT CTGTTGTAAC ACTGGAAGTA GCAAAACAAG GAGCAATATA CCACGGTCTG
    GCAACCCTGC TAAATCAGCC ATCTCCAATG ATGCAAAGAG TTTCTGACCG CCGTGGCTCA
    GGTAAACCCC GACCAAAGAG TGAAGGTTTT GAGCTGTATA ACAACTCCAC GCAAAATGGG
    TCACCTGAGA GCCCCCAGCT GCCATGGACG GAGTACAGTG AACCAAAGAA GCTGCCAGGA
    GATGACAGAT TAATGAAGAA CAGAGCCGAT CATCGCTCCA GCCCCAACGT AGCAAATCAG
    CCTCCAAGCC CTGGGGGGAA GAATGCTTAT GCATCTGGAA CTACCACCAA AATAACCTCA
    GTCTCGACTG GAAATCTTTG TACAGAGGAG CAAACTCTGC CTCCACGACC TGAAGCTTAT
    CCTATCCCAA CACAGACCTA TACCAGAGAA TATTTCACAT TCCCAGCTTC AAAATCCCAG
    GATCGAATGG GTCCAGCTCA GAGTCAGTGG CCAAATTATG AAGAGAAGCC ACAGATCCAG
    GCAGAAAGTA ATCACTCAAT CAATACTACA ATGCAGCGCG TGGCTCGATC CCAGGAAGAA
    CTCCGAGATA AAGTTTTCCA GCCAGAGAGG AATCGTTTGG AAGTTGTTAT CCGGAAGGAC
    ATAGAATACA TTGATCGGAA GTCAGACAGC GATCAGTGGA TGAATTCATC TGTGGATTCT
    AGCACATCTA GCCAAGAGCA CCTGAACCAT ACATCCAAAC CTGTTTCACC TGGTTCTTGC
    TTAACTAAAA GTGGACCTGG CCGTTGGAAA ACTCCAGCTA CGAGCCAGCC AACTCCAGTG
    GCCGTTTCCC AGCCCATCAG AACAGACCTT CCTCCTCCGC CGCCACCTCC TCCGGTGCAT
    TATGCAGCTG AATTTGATGA ACCACAAATG GATTTGCCTC TGCCTCCCCC TCCTCCACCG
    AACCAAGTAG GAGCACAAGC ATCTTCTCAG GTGGCTGCTG CTGAGCGTAA AAAACGAGAA
    GAGCATCAGA GGTGGTATGA AAAGGAAAAG GCACGTTTGG AAGAAGAACG AGAAAGGAAA
    CGCCGTGAGC AAGAGAGAAA ACTGGGCCAA ATGCGTTCGC AGCCACTAAT TCCTCCCCAG
    CCTGTGGTTC CTCCTGTTTC CCTTCAGCAA GTGAAATCGG AAAAAACTTC AACTTTCCAG
    AGGTCTCAAG AAACAGTCAT TCGAGAGTTG CAGCCGCAAC AGCAGCCTCG GACTATTGAG
    CGGAGAGATC TGCAGTACAT TACTATCAGC AAGGAAGAAT TGTCCTCTAG TGACAGCCTC
    TCTCCAGATC CCTGGAAACG AGATGCCAAG GAGAAACTGG AGAAGCAGCA GCAGATGCAC
    ATTGTGGACA TGCTGAGCAA AGAGATTCAG GATCTTCAGA ACAAGCCTGA TCGTACAGCT
    GAAGAGAACG ACCGCCTGCG CAAACTCATG CTAGAGTGGC AGTTCCAAAA GCGACTTCAG
    GAATCAAAAC AAAAGGATGA AGATGATGAT GAAGAAGAGG ACGATGATGT GGATACTATG
    CTTATCATGC AGCGGCTGGA GGCAGAAAGA AGAGCAAGGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGTCTGTACA GACCTCCACT TCCACGGGAA
    TATGAACTCC CATCCCCAAC CCCTTCATCT AACGCTCCTC CTCCCCCACC TCAGCGAAAC
    ACCTCTTACC TCAAAACACA GGTCCTCTCC CCTGACACGA TTTATACTGC CAAGTTTGTT
    GCATACAATG ATGATGAGGA GGAGGAGGAT TCCAATCTAG CAGGAATAAA TTCATACACT
    GGATCTTCGG GAGCAACGGT TGGGGCCCAT GAAGTCTATC GGGATCCAAG AGACAAACGA
    TCTAAAAGTC AAGATACAGA TTTACCTGGA GCACCAGAAA ATCTGACGTT CAGGGAACGC
    CAGCGACTTT TTTCACAGGG TCAAGATGTA TCCAATAAAG TAAAAGCCTC TAGGAAACTA
    ATGGAACTGG AAAATGAGTT GAACACGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33806
    Clone ID Related Accession (Same CDS sequence) XM_015284356.2 , XM_015284356.1
    Accession Version XM_015284356.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGAAACAGA AGAATATTGC TGGGGCACTT GCCTTTTGGA TGGCAAATGC GTCTGAATTG
    CTGAATTTTA TAAAGCAAGA CCGTGATCTG AGCCGAATCA CTGTGGATGC TCAAGATGTT
    TTGGCGCACT TGGTTCAAAT GGCATTCAAG TACCTAGTTC ATTGTCTGCA GTCAGAGCTT
    AATAACTACA TGCCAGCATT TCTTGATGAT CCAGAGGAAA ATAATCCACA GAGGCCTAAA
    ATAGATGATG TGCTGCACAC ATTGACTGGA GCTATGTCCT TGTTGCGACG ATGTAGAGTG
    AATGCTGCAC TGACTATACA GCTCTTCTCC CAGTTGTTTC ATTTTATCAA CATGTGGCTT
    TTTAACAGAC TAGTTACGGC TCCAGATTCA GGGTTATGTT CCCACTACTG GGGAGCTATT
    GTTCGTCAGC AGTTGGGTCA TATTGAGGCC TGGGCAGAAA AACAGGGTTT AGAATTGGCT
    GCTGATTGCC ACTTAAGCAG AATAGTGCAG GCAACCACAT TGCTCACCAT GGACAAGTAC
    TCACATGCTG ATGTTCCAAA TATTAACAGT ACTTGCTTTA AGTTGAATTC TCTACAGCTA
    CAAGCTTTGT TGCAGAATTA TCACTGTGCT CCAGATGAAC CGCTTATACC AACAGAACTG
    ATAGAGAATG TAGTAACTGT TGCAGAAAAT ACGGCTGATG AACTTGCTCG CAGTGATGGC
    AGAGAAGTCC AATTGGAAGA GGATCCTGAC TTACAACTCC CTTTTCTTTT ACCAGAAGAT
    GGCTATTCCT GTGATGTTGT CAGAAATGTT CCAAGTGGTT TACAAGAATT TTTGGATCCC
    CTCTGTCAGA GAGGTTTTTG TAGGTTAATA CCTCATACAC GGTCACCAGG CACATGGACA
    ATATACTTTG AAGGCGCAGA CTATGAAAGC CACCTGTCAC ATGAGAATGC TGAGCTAGCT
    CAGCCTTTGA GGAAAGAACC TGAGATTATA ACTGTAACAC TAAAAAAACA AAATGGAATG
    GGCCTCAGCA TTGTTGCTGC CAAGGGTGCT GGTCAAGACA AACTTGGAAT ATATGTCAAG
    TCTGTTGTGA AAGGAGGTGC TGCAGATGTG GATGGTCGTC TGGCAGCAGG GGATCAGCTG
    CTCAGTGTGG ATGGTCGAAG CTTGGTGGGG CTGTCCCAGG AAAGGGCAGC AGAACTTATG
    ACGAGGACAG GTTCTGTTGT AACACTGGAA GTAGCAAAAC AAGGAGCAAT ATACCACGGT
    CTGGCAACCC TGCTAAATCA GCCATCTCCA ATGATGCAAA GAGTTTCTGA CCGCCGTGGC
    TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTTGAGCTGT ATAACAACTC CACGCAAAAT
    GGGTCACCTG AGAGCCCCCA GCTGCCATGG ACGGAGTACA GTGAACCAAA GAAGCTGCCA
    GGAGATGACA GATTAATGAA GAACAGAGCC GATCATCGCT CCAGCCCCAA CGTAGCAAAT
    CAGCCTCCAA GCCCTGGGGG GAAGAATGCT TATGCATCTG GAACTACCAC CAAAATAACC
    TCAGTCTCGA CTGGAAATCT TTGTACAGAG GAGCAAACTC TGCCTCCACG ACCTGAAGCT
    TATCCTATCC CAACACAGAC CTATACCAGA GAATATTTCA CATTCCCAGC TTCAAAATCC
    CAGGATCGAA TGGGTCCAGC TCAGAGTCAG TGGCCAAATT ATGAAGAGAA GCCACAGATC
    CAGGCAGAAA GTAATCACTC AATCAATACT ACAATGCAGC GCGTGGCTCG ATCCCAGGAA
    GAACTCCGAG ATAAAGTTTT CCAGCCAGAG AGGAATCGTT TGGAAGTTGT TATCCGGAAG
    GACATAGAAT ACATTGATCG GAAGTCAGAC AGCGATCAGT GGATGAATTC ATCTGTGGAT
    TCTAGCACAT CTAGCCAAGA GCACCTGAAC CATACATCCA AACCTGTTTC ACCTGGTTCT
    TGCTTAACTA AAAGTGGACC TGGCCGTTGG AAAACTCCAG CTACGAGCCA GCCAACTCCA
    GTGGCCGTTT CCCAGCCCAT CAGAACAGAC CTTCCTCCTC CGCCGCCACC TCCTCCGGTG
    CATTATGCAG CTGAATTTGA TGAACCACAA ATGGATTTGC CTCTGCCTCC CCCTCCTCCA
    CCGAACCAAG TAGGAGCACA AGCATCTTCT CAGGTGGCTG CTGCTGAGCG TAAAAAACGA
    GAAGAGCATC AGAGGTGGTA TGAAAAGGAA AAGGCACGTT TGGAAGAAGA ACGAGAAAGG
    AAACGCCGTG AGCAAGAGAG AAAACTGGGC CAAATGCGTT CGCAGCCACT AATTCCTCCC
    CAGCCTGTGG TTCCTCCTGT TTCCCTTCAG CAAGTGAAAT CGGAAAAAAC TTCAACTTTC
    CAGAGGTCTC AAGAAACAGT CATTCGAGAG TTGCAGCCGC AACAGCAGCC TCGGACTATT
    GAGCGGAGAG ATCTGCAGTA CATTACTATC AGCAAGGAAG AATTGTCCTC TAGTGACAGC
    CTCTCTCCAG ATCCCTGGAA ACGAGATGCC AAGGAGAAAC TGGAGAAGCA GCAGCAGATG
    CACATTGTGG ACATGCTGAG CAAAGAGATT CAGGATCTTC AGAACAAGCC TGATCGTACA
    GCTGAAGAGA ACGACCGCCT GCGCAAACTC ATGCTAGAGT GGCAGTTCCA AAAGCGACTT
    CAGGAATCAA AACAAAAGGA TGAAGATGAT GATGAAGAAG AGGACGATGA TGTGGATACT
    ATGCTTATCA TGCAGCGGCT GGAGGCAGAA AGAAGAGCAA GGGATGAAGA ACGCAGACGA
    CAGCAACAGT TAGAAGAAAT GCGCAAGCGA GAAGCAGAAG ACAGGGCCAG GCAGGAAGAG
    GAGCGCCGAC AGCAAGAGGA GGAGCGAACC AGGAGAGAGG CAGAAGAAAA GAGGCGACAG
    GAAGAGGAGT ATTACAATCG CTTAGAGGCT GAAAGGCGCA GGCAGCACGA TGAAGCAGAA
    CGAAGATTGC TGGAACCAGA CGAGCCTGGT CTGTACAGAC CTCCACTTCC ACGGGAATAT
    GAACTCCCAT CCCCAACCCC TTCATCTAAC GCTCCTCCTC CCCCACCTCA GCGAAACACC
    TCTTACCTCA AAACACAGGT CCTCTCCCCT GACACGATTT ATACTGCCAA GTTTGTTGCA
    TACAATGATG ATGAGGAGGA GGAGGATTCC AATCTAGCAG GAATAAATTC ATACACTGGA
    TCTTCGGGAG CAACGGTTGG GGCCCATGAA GTCTATCGGG ATCCAAGAGA CAAACGATCT
    AAAAGTCAAG ATACAGATTT ACCTGGAGCA CCAGAAAATC TGACGTTCAG GGAACGCCAG
    CGACTTTTTT CACAGGGTCA AGATGTATCC AATAAAGTAA AAGCCTCTAG GAAACTAATG
    GAACTGGAAA ATGAGTTGAA CACGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139842.1
    CDS2885..8374
    Translation

    Target ORF information:

    RefSeq Version XM_015284356.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284356.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGAAACAGA AGAATATTGC TGGGGCACTT GCCTTTTGGA TGGCAAATGC GTCTGAATTG
    CTGAATTTTA TAAAGCAAGA CCGTGATCTG AGCCGAATCA CTGTGGATGC TCAAGATGTT
    TTGGCGCACT TGGTTCAAAT GGCATTCAAG TACCTAGTTC ATTGTCTGCA GTCAGAGCTT
    AATAACTACA TGCCAGCATT TCTTGATGAT CCAGAGGAAA ATAATCCACA GAGGCCTAAA
    ATAGATGATG TGCTGCACAC ATTGACTGGA GCTATGTCCT TGTTGCGACG ATGTAGAGTG
    AATGCTGCAC TGACTATACA GCTCTTCTCC CAGTTGTTTC ATTTTATCAA CATGTGGCTT
    TTTAACAGAC TAGTTACGGC TCCAGATTCA GGGTTATGTT CCCACTACTG GGGAGCTATT
    GTTCGTCAGC AGTTGGGTCA TATTGAGGCC TGGGCAGAAA AACAGGGTTT AGAATTGGCT
    GCTGATTGCC ACTTAAGCAG AATAGTGCAG GCAACCACAT TGCTCACCAT GGACAAGTAC
    TCACATGCTG ATGTTCCAAA TATTAACAGT ACTTGCTTTA AGTTGAATTC TCTACAGCTA
    CAAGCTTTGT TGCAGAATTA TCACTGTGCT CCAGATGAAC CGCTTATACC AACAGAACTG
    ATAGAGAATG TAGTAACTGT TGCAGAAAAT ACGGCTGATG AACTTGCTCG CAGTGATGGC
    AGAGAAGTCC AATTGGAAGA GGATCCTGAC TTACAACTCC CTTTTCTTTT ACCAGAAGAT
    GGCTATTCCT GTGATGTTGT CAGAAATGTT CCAAGTGGTT TACAAGAATT TTTGGATCCC
    CTCTGTCAGA GAGGTTTTTG TAGGTTAATA CCTCATACAC GGTCACCAGG CACATGGACA
    ATATACTTTG AAGGCGCAGA CTATGAAAGC CACCTGTCAC ATGAGAATGC TGAGCTAGCT
    CAGCCTTTGA GGAAAGAACC TGAGATTATA ACTGTAACAC TAAAAAAACA AAATGGAATG
    GGCCTCAGCA TTGTTGCTGC CAAGGGTGCT GGTCAAGACA AACTTGGAAT ATATGTCAAG
    TCTGTTGTGA AAGGAGGTGC TGCAGATGTG GATGGTCGTC TGGCAGCAGG GGATCAGCTG
    CTCAGTGTGG ATGGTCGAAG CTTGGTGGGG CTGTCCCAGG AAAGGGCAGC AGAACTTATG
    ACGAGGACAG GTTCTGTTGT AACACTGGAA GTAGCAAAAC AAGGAGCAAT ATACCACGGT
    CTGGCAACCC TGCTAAATCA GCCATCTCCA ATGATGCAAA GAGTTTCTGA CCGCCGTGGC
    TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTTGAGCTGT ATAACAACTC CACGCAAAAT
    GGGTCACCTG AGAGCCCCCA GCTGCCATGG ACGGAGTACA GTGAACCAAA GAAGCTGCCA
    GGAGATGACA GATTAATGAA GAACAGAGCC GATCATCGCT CCAGCCCCAA CGTAGCAAAT
    CAGCCTCCAA GCCCTGGGGG GAAGAATGCT TATGCATCTG GAACTACCAC CAAAATAACC
    TCAGTCTCGA CTGGAAATCT TTGTACAGAG GAGCAAACTC TGCCTCCACG ACCTGAAGCT
    TATCCTATCC CAACACAGAC CTATACCAGA GAATATTTCA CATTCCCAGC TTCAAAATCC
    CAGGATCGAA TGGGTCCAGC TCAGAGTCAG TGGCCAAATT ATGAAGAGAA GCCACAGATC
    CAGGCAGAAA GTAATCACTC AATCAATACT ACAATGCAGC GCGTGGCTCG ATCCCAGGAA
    GAACTCCGAG ATAAAGTTTT CCAGCCAGAG AGGAATCGTT TGGAAGTTGT TATCCGGAAG
    GACATAGAAT ACATTGATCG GAAGTCAGAC AGCGATCAGT GGATGAATTC ATCTGTGGAT
    TCTAGCACAT CTAGCCAAGA GCACCTGAAC CATACATCCA AACCTGTTTC ACCTGGTTCT
    TGCTTAACTA AAAGTGGACC TGGCCGTTGG AAAACTCCAG CTACGAGCCA GCCAACTCCA
    GTGGCCGTTT CCCAGCCCAT CAGAACAGAC CTTCCTCCTC CGCCGCCACC TCCTCCGGTG
    CATTATGCAG CTGAATTTGA TGAACCACAA ATGGATTTGC CTCTGCCTCC CCCTCCTCCA
    CCGAACCAAG TAGGAGCACA AGCATCTTCT CAGGTGGCTG CTGCTGAGCG TAAAAAACGA
    GAAGAGCATC AGAGGTGGTA TGAAAAGGAA AAGGCACGTT TGGAAGAAGA ACGAGAAAGG
    AAACGCCGTG AGCAAGAGAG AAAACTGGGC CAAATGCGTT CGCAGCCACT AATTCCTCCC
    CAGCCTGTGG TTCCTCCTGT TTCCCTTCAG CAAGTGAAAT CGGAAAAAAC TTCAACTTTC
    CAGAGGTCTC AAGAAACAGT CATTCGAGAG TTGCAGCCGC AACAGCAGCC TCGGACTATT
    GAGCGGAGAG ATCTGCAGTA CATTACTATC AGCAAGGAAG AATTGTCCTC TAGTGACAGC
    CTCTCTCCAG ATCCCTGGAA ACGAGATGCC AAGGAGAAAC TGGAGAAGCA GCAGCAGATG
    CACATTGTGG ACATGCTGAG CAAAGAGATT CAGGATCTTC AGAACAAGCC TGATCGTACA
    GCTGAAGAGA ACGACCGCCT GCGCAAACTC ATGCTAGAGT GGCAGTTCCA AAAGCGACTT
    CAGGAATCAA AACAAAAGGA TGAAGATGAT GATGAAGAAG AGGACGATGA TGTGGATACT
    ATGCTTATCA TGCAGCGGCT GGAGGCAGAA AGAAGAGCAA GGGATGAAGA ACGCAGACGA
    CAGCAACAGT TAGAAGAAAT GCGCAAGCGA GAAGCAGAAG ACAGGGCCAG GCAGGAAGAG
    GAGCGCCGAC AGCAAGAGGA GGAGCGAACC AGGAGAGAGG CAGAAGAAAA GAGGCGACAG
    GAAGAGGAGT ATTACAATCG CTTAGAGGCT GAAAGGCGCA GGCAGCACGA TGAAGCAGAA
    CGAAGATTGC TGGAACCAGA CGAGCCTGGT CTGTACAGAC CTCCACTTCC ACGGGAATAT
    GAACTCCCAT CCCCAACCCC TTCATCTAAC GCTCCTCCTC CCCCACCTCA GCGAAACACC
    TCTTACCTCA AAACACAGGT CCTCTCCCCT GACACGATTT ATACTGCCAA GTTTGTTGCA
    TACAATGATG ATGAGGAGGA GGAGGATTCC AATCTAGCAG GAATAAATTC ATACACTGGA
    TCTTCGGGAG CAACGGTTGG GGCCCATGAA GTCTATCGGG ATCCAAGAGA CAAACGATCT
    AAAAGTCAAG ATACAGATTT ACCTGGAGCA CCAGAAAATC TGACGTTCAG GGAACGCCAG
    CGACTTTTTT CACAGGGTCA AGATGTATCC AATAAAGTAA AAGCCTCTAG GAAACTAATG
    GAACTGGAAA ATGAGTTGAA CACGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33806
    Clone ID Related Accession (Same CDS sequence) XM_015284356.2 , XM_015284356.1
    Accession Version XM_015284356.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGAAACAGA AGAATATTGC TGGGGCACTT GCCTTTTGGA TGGCAAATGC GTCTGAATTG
    CTGAATTTTA TAAAGCAAGA CCGTGATCTG AGCCGAATCA CTGTGGATGC TCAAGATGTT
    TTGGCGCACT TGGTTCAAAT GGCATTCAAG TACCTAGTTC ATTGTCTGCA GTCAGAGCTT
    AATAACTACA TGCCAGCATT TCTTGATGAT CCAGAGGAAA ATAATCCACA GAGGCCTAAA
    ATAGATGATG TGCTGCACAC ATTGACTGGA GCTATGTCCT TGTTGCGACG ATGTAGAGTG
    AATGCTGCAC TGACTATACA GCTCTTCTCC CAGTTGTTTC ATTTTATCAA CATGTGGCTT
    TTTAACAGAC TAGTTACGGC TCCAGATTCA GGGTTATGTT CCCACTACTG GGGAGCTATT
    GTTCGTCAGC AGTTGGGTCA TATTGAGGCC TGGGCAGAAA AACAGGGTTT AGAATTGGCT
    GCTGATTGCC ACTTAAGCAG AATAGTGCAG GCAACCACAT TGCTCACCAT GGACAAGTAC
    TCACATGCTG ATGTTCCAAA TATTAACAGT ACTTGCTTTA AGTTGAATTC TCTACAGCTA
    CAAGCTTTGT TGCAGAATTA TCACTGTGCT CCAGATGAAC CGCTTATACC AACAGAACTG
    ATAGAGAATG TAGTAACTGT TGCAGAAAAT ACGGCTGATG AACTTGCTCG CAGTGATGGC
    AGAGAAGTCC AATTGGAAGA GGATCCTGAC TTACAACTCC CTTTTCTTTT ACCAGAAGAT
    GGCTATTCCT GTGATGTTGT CAGAAATGTT CCAAGTGGTT TACAAGAATT TTTGGATCCC
    CTCTGTCAGA GAGGTTTTTG TAGGTTAATA CCTCATACAC GGTCACCAGG CACATGGACA
    ATATACTTTG AAGGCGCAGA CTATGAAAGC CACCTGTCAC ATGAGAATGC TGAGCTAGCT
    CAGCCTTTGA GGAAAGAACC TGAGATTATA ACTGTAACAC TAAAAAAACA AAATGGAATG
    GGCCTCAGCA TTGTTGCTGC CAAGGGTGCT GGTCAAGACA AACTTGGAAT ATATGTCAAG
    TCTGTTGTGA AAGGAGGTGC TGCAGATGTG GATGGTCGTC TGGCAGCAGG GGATCAGCTG
    CTCAGTGTGG ATGGTCGAAG CTTGGTGGGG CTGTCCCAGG AAAGGGCAGC AGAACTTATG
    ACGAGGACAG GTTCTGTTGT AACACTGGAA GTAGCAAAAC AAGGAGCAAT ATACCACGGT
    CTGGCAACCC TGCTAAATCA GCCATCTCCA ATGATGCAAA GAGTTTCTGA CCGCCGTGGC
    TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTTGAGCTGT ATAACAACTC CACGCAAAAT
    GGGTCACCTG AGAGCCCCCA GCTGCCATGG ACGGAGTACA GTGAACCAAA GAAGCTGCCA
    GGAGATGACA GATTAATGAA GAACAGAGCC GATCATCGCT CCAGCCCCAA CGTAGCAAAT
    CAGCCTCCAA GCCCTGGGGG GAAGAATGCT TATGCATCTG GAACTACCAC CAAAATAACC
    TCAGTCTCGA CTGGAAATCT TTGTACAGAG GAGCAAACTC TGCCTCCACG ACCTGAAGCT
    TATCCTATCC CAACACAGAC CTATACCAGA GAATATTTCA CATTCCCAGC TTCAAAATCC
    CAGGATCGAA TGGGTCCAGC TCAGAGTCAG TGGCCAAATT ATGAAGAGAA GCCACAGATC
    CAGGCAGAAA GTAATCACTC AATCAATACT ACAATGCAGC GCGTGGCTCG ATCCCAGGAA
    GAACTCCGAG ATAAAGTTTT CCAGCCAGAG AGGAATCGTT TGGAAGTTGT TATCCGGAAG
    GACATAGAAT ACATTGATCG GAAGTCAGAC AGCGATCAGT GGATGAATTC ATCTGTGGAT
    TCTAGCACAT CTAGCCAAGA GCACCTGAAC CATACATCCA AACCTGTTTC ACCTGGTTCT
    TGCTTAACTA AAAGTGGACC TGGCCGTTGG AAAACTCCAG CTACGAGCCA GCCAACTCCA
    GTGGCCGTTT CCCAGCCCAT CAGAACAGAC CTTCCTCCTC CGCCGCCACC TCCTCCGGTG
    CATTATGCAG CTGAATTTGA TGAACCACAA ATGGATTTGC CTCTGCCTCC CCCTCCTCCA
    CCGAACCAAG TAGGAGCACA AGCATCTTCT CAGGTGGCTG CTGCTGAGCG TAAAAAACGA
    GAAGAGCATC AGAGGTGGTA TGAAAAGGAA AAGGCACGTT TGGAAGAAGA ACGAGAAAGG
    AAACGCCGTG AGCAAGAGAG AAAACTGGGC CAAATGCGTT CGCAGCCACT AATTCCTCCC
    CAGCCTGTGG TTCCTCCTGT TTCCCTTCAG CAAGTGAAAT CGGAAAAAAC TTCAACTTTC
    CAGAGGTCTC AAGAAACAGT CATTCGAGAG TTGCAGCCGC AACAGCAGCC TCGGACTATT
    GAGCGGAGAG ATCTGCAGTA CATTACTATC AGCAAGGAAG AATTGTCCTC TAGTGACAGC
    CTCTCTCCAG ATCCCTGGAA ACGAGATGCC AAGGAGAAAC TGGAGAAGCA GCAGCAGATG
    CACATTGTGG ACATGCTGAG CAAAGAGATT CAGGATCTTC AGAACAAGCC TGATCGTACA
    GCTGAAGAGA ACGACCGCCT GCGCAAACTC ATGCTAGAGT GGCAGTTCCA AAAGCGACTT
    CAGGAATCAA AACAAAAGGA TGAAGATGAT GATGAAGAAG AGGACGATGA TGTGGATACT
    ATGCTTATCA TGCAGCGGCT GGAGGCAGAA AGAAGAGCAA GGGATGAAGA ACGCAGACGA
    CAGCAACAGT TAGAAGAAAT GCGCAAGCGA GAAGCAGAAG ACAGGGCCAG GCAGGAAGAG
    GAGCGCCGAC AGCAAGAGGA GGAGCGAACC AGGAGAGAGG CAGAAGAAAA GAGGCGACAG
    GAAGAGGAGT ATTACAATCG CTTAGAGGCT GAAAGGCGCA GGCAGCACGA TGAAGCAGAA
    CGAAGATTGC TGGAACCAGA CGAGCCTGGT CTGTACAGAC CTCCACTTCC ACGGGAATAT
    GAACTCCCAT CCCCAACCCC TTCATCTAAC GCTCCTCCTC CCCCACCTCA GCGAAACACC
    TCTTACCTCA AAACACAGGT CCTCTCCCCT GACACGATTT ATACTGCCAA GTTTGTTGCA
    TACAATGATG ATGAGGAGGA GGAGGATTCC AATCTAGCAG GAATAAATTC ATACACTGGA
    TCTTCGGGAG CAACGGTTGG GGCCCATGAA GTCTATCGGG ATCCAAGAGA CAAACGATCT
    AAAAGTCAAG ATACAGATTT ACCTGGAGCA CCAGAAAATC TGACGTTCAG GGAACGCCAG
    CGACTTTTTT CACAGGGTCA AGATGTATCC AATAAAGTAA AAGCCTCTAG GAAACTAATG
    GAACTGGAAA ATGAGTTGAA CACGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139842.1
    CDS33..5522
    Translation

    Target ORF information:

    RefSeq Version XM_015284356.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284356.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGAAACAGA AGAATATTGC TGGGGCACTT GCCTTTTGGA TGGCAAATGC GTCTGAATTG
    CTGAATTTTA TAAAGCAAGA CCGTGATCTG AGCCGAATCA CTGTGGATGC TCAAGATGTT
    TTGGCGCACT TGGTTCAAAT GGCATTCAAG TACCTAGTTC ATTGTCTGCA GTCAGAGCTT
    AATAACTACA TGCCAGCATT TCTTGATGAT CCAGAGGAAA ATAATCCACA GAGGCCTAAA
    ATAGATGATG TGCTGCACAC ATTGACTGGA GCTATGTCCT TGTTGCGACG ATGTAGAGTG
    AATGCTGCAC TGACTATACA GCTCTTCTCC CAGTTGTTTC ATTTTATCAA CATGTGGCTT
    TTTAACAGAC TAGTTACGGC TCCAGATTCA GGGTTATGTT CCCACTACTG GGGAGCTATT
    GTTCGTCAGC AGTTGGGTCA TATTGAGGCC TGGGCAGAAA AACAGGGTTT AGAATTGGCT
    GCTGATTGCC ACTTAAGCAG AATAGTGCAG GCAACCACAT TGCTCACCAT GGACAAGTAC
    TCACATGCTG ATGTTCCAAA TATTAACAGT ACTTGCTTTA AGTTGAATTC TCTACAGCTA
    CAAGCTTTGT TGCAGAATTA TCACTGTGCT CCAGATGAAC CGCTTATACC AACAGAACTG
    ATAGAGAATG TAGTAACTGT TGCAGAAAAT ACGGCTGATG AACTTGCTCG CAGTGATGGC
    AGAGAAGTCC AATTGGAAGA GGATCCTGAC TTACAACTCC CTTTTCTTTT ACCAGAAGAT
    GGCTATTCCT GTGATGTTGT CAGAAATGTT CCAAGTGGTT TACAAGAATT TTTGGATCCC
    CTCTGTCAGA GAGGTTTTTG TAGGTTAATA CCTCATACAC GGTCACCAGG CACATGGACA
    ATATACTTTG AAGGCGCAGA CTATGAAAGC CACCTGTCAC ATGAGAATGC TGAGCTAGCT
    CAGCCTTTGA GGAAAGAACC TGAGATTATA ACTGTAACAC TAAAAAAACA AAATGGAATG
    GGCCTCAGCA TTGTTGCTGC CAAGGGTGCT GGTCAAGACA AACTTGGAAT ATATGTCAAG
    TCTGTTGTGA AAGGAGGTGC TGCAGATGTG GATGGTCGTC TGGCAGCAGG GGATCAGCTG
    CTCAGTGTGG ATGGTCGAAG CTTGGTGGGG CTGTCCCAGG AAAGGGCAGC AGAACTTATG
    ACGAGGACAG GTTCTGTTGT AACACTGGAA GTAGCAAAAC AAGGAGCAAT ATACCACGGT
    CTGGCAACCC TGCTAAATCA GCCATCTCCA ATGATGCAAA GAGTTTCTGA CCGCCGTGGC
    TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTTGAGCTGT ATAACAACTC CACGCAAAAT
    GGGTCACCTG AGAGCCCCCA GCTGCCATGG ACGGAGTACA GTGAACCAAA GAAGCTGCCA
    GGAGATGACA GATTAATGAA GAACAGAGCC GATCATCGCT CCAGCCCCAA CGTAGCAAAT
    CAGCCTCCAA GCCCTGGGGG GAAGAATGCT TATGCATCTG GAACTACCAC CAAAATAACC
    TCAGTCTCGA CTGGAAATCT TTGTACAGAG GAGCAAACTC TGCCTCCACG ACCTGAAGCT
    TATCCTATCC CAACACAGAC CTATACCAGA GAATATTTCA CATTCCCAGC TTCAAAATCC
    CAGGATCGAA TGGGTCCAGC TCAGAGTCAG TGGCCAAATT ATGAAGAGAA GCCACAGATC
    CAGGCAGAAA GTAATCACTC AATCAATACT ACAATGCAGC GCGTGGCTCG ATCCCAGGAA
    GAACTCCGAG ATAAAGTTTT CCAGCCAGAG AGGAATCGTT TGGAAGTTGT TATCCGGAAG
    GACATAGAAT ACATTGATCG GAAGTCAGAC AGCGATCAGT GGATGAATTC ATCTGTGGAT
    TCTAGCACAT CTAGCCAAGA GCACCTGAAC CATACATCCA AACCTGTTTC ACCTGGTTCT
    TGCTTAACTA AAAGTGGACC TGGCCGTTGG AAAACTCCAG CTACGAGCCA GCCAACTCCA
    GTGGCCGTTT CCCAGCCCAT CAGAACAGAC CTTCCTCCTC CGCCGCCACC TCCTCCGGTG
    CATTATGCAG CTGAATTTGA TGAACCACAA ATGGATTTGC CTCTGCCTCC CCCTCCTCCA
    CCGAACCAAG TAGGAGCACA AGCATCTTCT CAGGTGGCTG CTGCTGAGCG TAAAAAACGA
    GAAGAGCATC AGAGGTGGTA TGAAAAGGAA AAGGCACGTT TGGAAGAAGA ACGAGAAAGG
    AAACGCCGTG AGCAAGAGAG AAAACTGGGC CAAATGCGTT CGCAGCCACT AATTCCTCCC
    CAGCCTGTGG TTCCTCCTGT TTCCCTTCAG CAAGTGAAAT CGGAAAAAAC TTCAACTTTC
    CAGAGGTCTC AAGAAACAGT CATTCGAGAG TTGCAGCCGC AACAGCAGCC TCGGACTATT
    GAGCGGAGAG ATCTGCAGTA CATTACTATC AGCAAGGAAG AATTGTCCTC TAGTGACAGC
    CTCTCTCCAG ATCCCTGGAA ACGAGATGCC AAGGAGAAAC TGGAGAAGCA GCAGCAGATG
    CACATTGTGG ACATGCTGAG CAAAGAGATT CAGGATCTTC AGAACAAGCC TGATCGTACA
    GCTGAAGAGA ACGACCGCCT GCGCAAACTC ATGCTAGAGT GGCAGTTCCA AAAGCGACTT
    CAGGAATCAA AACAAAAGGA TGAAGATGAT GATGAAGAAG AGGACGATGA TGTGGATACT
    ATGCTTATCA TGCAGCGGCT GGAGGCAGAA AGAAGAGCAA GGGATGAAGA ACGCAGACGA
    CAGCAACAGT TAGAAGAAAT GCGCAAGCGA GAAGCAGAAG ACAGGGCCAG GCAGGAAGAG
    GAGCGCCGAC AGCAAGAGGA GGAGCGAACC AGGAGAGAGG CAGAAGAAAA GAGGCGACAG
    GAAGAGGAGT ATTACAATCG CTTAGAGGCT GAAAGGCGCA GGCAGCACGA TGAAGCAGAA
    CGAAGATTGC TGGAACCAGA CGAGCCTGGT CTGTACAGAC CTCCACTTCC ACGGGAATAT
    GAACTCCCAT CCCCAACCCC TTCATCTAAC GCTCCTCCTC CCCCACCTCA GCGAAACACC
    TCTTACCTCA AAACACAGGT CCTCTCCCCT GACACGATTT ATACTGCCAA GTTTGTTGCA
    TACAATGATG ATGAGGAGGA GGAGGATTCC AATCTAGCAG GAATAAATTC ATACACTGGA
    TCTTCGGGAG CAACGGTTGG GGCCCATGAA GTCTATCGGG ATCCAAGAGA CAAACGATCT
    AAAAGTCAAG ATACAGATTT ACCTGGAGCA CCAGAAAATC TGACGTTCAG GGAACGCCAG
    CGACTTTTTT CACAGGGTCA AGATGTATCC AATAAAGTAA AAGCCTCTAG GAAACTAATG
    GAACTGGAAA ATGAGTTGAA CACGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33807
    Clone ID Related Accession (Same CDS sequence) XM_015284357.1 , XM_015284357.2
    Accession Version XM_015284357.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5361bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGAATAAATT CATACACTGG ATCTTCGGGA
    GCAACGGTTG GGGCCCATGA AGTCTATCGG GATCCAAGAG ACAAACGATC TAAAAGTCAA
    GATACAGATT TACCTGGAGC ACCAGAAAAT CTGACGTTCA GGGAACGCCA GCGACTTTTT
    TCACAGGGTC AAGATGTATC CAATAAAGTA AAAGCCTCTA GGAAACTAAT GGAACTGGAA
    AATGAGTTGA ACACGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139843.1
    CDS33..5393
    Translation

    Target ORF information:

    RefSeq Version XM_015284357.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284357.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGAATAAATT CATACACTGG ATCTTCGGGA
    GCAACGGTTG GGGCCCATGA AGTCTATCGG GATCCAAGAG ACAAACGATC TAAAAGTCAA
    GATACAGATT TACCTGGAGC ACCAGAAAAT CTGACGTTCA GGGAACGCCA GCGACTTTTT
    TCACAGGGTC AAGATGTATC CAATAAAGTA AAAGCCTCTA GGAAACTAAT GGAACTGGAA
    AATGAGTTGA ACACGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33807
    Clone ID Related Accession (Same CDS sequence) XM_015284357.1 , XM_015284357.2
    Accession Version XM_015284357.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5361bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGAATAAATT CATACACTGG ATCTTCGGGA
    GCAACGGTTG GGGCCCATGA AGTCTATCGG GATCCAAGAG ACAAACGATC TAAAAGTCAA
    GATACAGATT TACCTGGAGC ACCAGAAAAT CTGACGTTCA GGGAACGCCA GCGACTTTTT
    TCACAGGGTC AAGATGTATC CAATAAAGTA AAAGCCTCTA GGAAACTAAT GGAACTGGAA
    AATGAGTTGA ACACGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139843.1
    CDS2885..8245
    Translation

    Target ORF information:

    RefSeq Version XM_015284357.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284357.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGAATAAATT CATACACTGG ATCTTCGGGA
    GCAACGGTTG GGGCCCATGA AGTCTATCGG GATCCAAGAG ACAAACGATC TAAAAGTCAA
    GATACAGATT TACCTGGAGC ACCAGAAAAT CTGACGTTCA GGGAACGCCA GCGACTTTTT
    TCACAGGGTC AAGATGTATC CAATAAAGTA AAAGCCTCTA GGAAACTAAT GGAACTGGAA
    AATGAGTTGA ACACGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33808
    Clone ID Related Accession (Same CDS sequence) XM_015284358.1 , XM_015284358.2
    Accession Version XM_015284358.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGTTACAGG ATGAAGAACG CAGACGACAG CAACAGTTAG AAGAAATGCG CAAGCGAGAA
    GCAGAAGACA GGGCCAGGCA GGAAGAGGAG CGCCGACAGC AAGAGGAGGA GCGAACCAGG
    AGAGAGGCAG AAGAAAAGAG GCGACAGGAA GAGGAGTATT ACAATCGCTT AGAGGCTGAA
    AGGCGCAGGC AGCACGATGA AGCAGAACGA AGATTGCTGG AACCAGACGA GCCTGGAATA
    AATTCATACA CTGGATCTTC GGGAGCAACG GTTGGGGCCC ATGAAGTCTA TCGGGATCCA
    AGAGACAAAC GATCTAAAAG TCAAGATACA GATTTACCTG GAGCACCAGA AAATCTGACG
    TTCAGGGAAC GCCAGCGACT TTTTTCACAG GGTCAAGATG TATCCAATAA AGTAAAAGCC
    TCTAGGAAAC TAATGGAACT GGAAAATGAG TTGAACACGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139844.1
    CDS33..5357
    Translation

    Target ORF information:

    RefSeq Version XM_015284358.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284358.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGTTACAGG ATGAAGAACG CAGACGACAG CAACAGTTAG AAGAAATGCG CAAGCGAGAA
    GCAGAAGACA GGGCCAGGCA GGAAGAGGAG CGCCGACAGC AAGAGGAGGA GCGAACCAGG
    AGAGAGGCAG AAGAAAAGAG GCGACAGGAA GAGGAGTATT ACAATCGCTT AGAGGCTGAA
    AGGCGCAGGC AGCACGATGA AGCAGAACGA AGATTGCTGG AACCAGACGA GCCTGGAATA
    AATTCATACA CTGGATCTTC GGGAGCAACG GTTGGGGCCC ATGAAGTCTA TCGGGATCCA
    AGAGACAAAC GATCTAAAAG TCAAGATACA GATTTACCTG GAGCACCAGA AAATCTGACG
    TTCAGGGAAC GCCAGCGACT TTTTTCACAG GGTCAAGATG TATCCAATAA AGTAAAAGCC
    TCTAGGAAAC TAATGGAACT GGAAAATGAG TTGAACACGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33808
    Clone ID Related Accession (Same CDS sequence) XM_015284358.1 , XM_015284358.2
    Accession Version XM_015284358.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGTTACAGG ATGAAGAACG CAGACGACAG CAACAGTTAG AAGAAATGCG CAAGCGAGAA
    GCAGAAGACA GGGCCAGGCA GGAAGAGGAG CGCCGACAGC AAGAGGAGGA GCGAACCAGG
    AGAGAGGCAG AAGAAAAGAG GCGACAGGAA GAGGAGTATT ACAATCGCTT AGAGGCTGAA
    AGGCGCAGGC AGCACGATGA AGCAGAACGA AGATTGCTGG AACCAGACGA GCCTGGAATA
    AATTCATACA CTGGATCTTC GGGAGCAACG GTTGGGGCCC ATGAAGTCTA TCGGGATCCA
    AGAGACAAAC GATCTAAAAG TCAAGATACA GATTTACCTG GAGCACCAGA AAATCTGACG
    TTCAGGGAAC GCCAGCGACT TTTTTCACAG GGTCAAGATG TATCCAATAA AGTAAAAGCC
    TCTAGGAAAC TAATGGAACT GGAAAATGAG TTGAACACGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139844.1
    CDS2885..8209
    Translation

    Target ORF information:

    RefSeq Version XM_015284358.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284358.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGTTACAGG ATGAAGAACG CAGACGACAG CAACAGTTAG AAGAAATGCG CAAGCGAGAA
    GCAGAAGACA GGGCCAGGCA GGAAGAGGAG CGCCGACAGC AAGAGGAGGA GCGAACCAGG
    AGAGAGGCAG AAGAAAAGAG GCGACAGGAA GAGGAGTATT ACAATCGCTT AGAGGCTGAA
    AGGCGCAGGC AGCACGATGA AGCAGAACGA AGATTGCTGG AACCAGACGA GCCTGGAATA
    AATTCATACA CTGGATCTTC GGGAGCAACG GTTGGGGCCC ATGAAGTCTA TCGGGATCCA
    AGAGACAAAC GATCTAAAAG TCAAGATACA GATTTACCTG GAGCACCAGA AAATCTGACG
    TTCAGGGAAC GCCAGCGACT TTTTTCACAG GGTCAAGATG TATCCAATAA AGTAAAAGCC
    TCTAGGAAAC TAATGGAACT GGAAAATGAG TTGAACACGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33809
    Clone ID Related Accession (Same CDS sequence) XM_015284359.1 , XM_015284359.2
    Accession Version XM_015284359.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGTCCTCTCC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139845.1
    CDS33..5177
    Translation

    Target ORF information:

    RefSeq Version XM_015284359.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284359.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGTCCTCTCC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33809
    Clone ID Related Accession (Same CDS sequence) XM_015284359.1 , XM_015284359.2
    Accession Version XM_015284359.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGTCCTCTCC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139845.1
    CDS2885..8029
    Translation

    Target ORF information:

    RefSeq Version XM_015284359.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284359.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAGAAA GAAGATTAGA TGTGGATGAG AAGCCTCTTG TTGTACAATT AAACTGGAAC
    AAAGATGATC GAGAAGGAAG GTTTGTTCTC AAGAATGAAA ATGATACGCT TCCTCCAAAG
    AAGGCTCAGA GTAATGGCCC TGAAAAACAA GAAAAAGAAG GAGTAATACA GAATTTCAAA
    CGAACTCTCT CAAAGAAAGA AAAGAAAGAG AAGAAGAGGC GTGAAAAAGA GGCATTGCGA
    CAAGCATCAG ATAAAGATGA TCGACTTCTT CATGGGGATG ATATTGAGAA TTCTCGCCTT
    GCAGCTGAGG TTTACAAAGA TATGCCTGAG ACCAGCTTCA CTCGCACAAT ATCTAATCCT
    GAGGTGGTTA TGAAACGACG GCGGCAGCAA AAACTAGAGA AGAGAATGCA GGAATTTCGC
    AGTTCTGATG GCAGACCAGA CTCAGGTGGG ACGCTGAGAA TTTATGCGGA CAGTTTAAAA
    CCAAATATAC CATACAAGAC AATCCTGTTG TCAACTACAG ATACTGCAGA CTTTGCAGTT
    ATTGAAGCAC TGGAGAAATA TGGCTTGGAG AAGGAAAACC CCAAGGATTA TTGTATTGCT
    CGTGTCATTC TGCCTCCTGG TGCTCAGCAC TCTGATGATA AAGGTGCTAA AGAAACTATT
    CTAGATGATG ATGAGTGTCC ACTTCAGATA TTCAGGGAAT GGCCTAGTGA TAAAGGAATA
    CTAGTCTTTC AGTTGAAAAG GCGGCCTCCT GATTATGTCC CAAAGAAATC CAAGAAAATA
    GCTGATGGAA AGCCATTAAA GGGGAAGGAA AGAGTTGACG GGTCTGGCTA CGGCTCTTGC
    CTTCCTCCTG AGAAACTACC ATACCTGGTA GAATTAAGCC CAGGGAGAAG GAATCACTTT
    GCCTACTACA ACTATCACGC TTATGAAGAT GGTTCTGACT CCAGAGATAA ACCAAAGCTT
    TATCGTCTAC AATTAAGTGT CACTGAAGTT GGAACTGAAA AATTTGATGA CAATTCCATT
    CAGTTATTTG GTCCAGGAAT TCAGCCTCAT CATTGTGATC TTACTAATAT GGATGGAGTT
    GTTACTGTAA CCCCAAGAAA CATTGATGCG GAAACGTATG TAGAAGGTCA GCGTATTTCA
    GAAACCACTA TGCTGCAAAG TGGAATGAAG GTTCAGTTTG GGTCATCTCA TGTGTTTAAG
    TTTGTGGATC CCACTCAGGA CCATATTATT ACTAAAAGAC CTATTGATGC AAGCCTTATG
    GTCAAAGGTC CAAGGCATAA AGCTGGAGCT GTTCAGGAAA CCACGTTTGA TCTGGAGGGA
    GATGTTCACA GTGGAACAGC GTTACCAGCG AGCAAGAGCA CCAGCAGGCT TGATGGTGAC
    AGAACATCAT CAGCATCCAG CACAGCTGAA CGAGGAATGG TGAAGCCCAT GATTAGAATA
    GAACAGCAGC AAGATTATCG CAGACAGGAA AGCAGACCTC AGGATACCCA TGGACCAGAA
    CTGATCCTAC CTGCCAGTAT TGAATTCAGG GAAAGCTCTG AAGATGCCTT CTTATCTGCC
    ATTATAAATT ATACAAATAG CTCGACAGTT CATTTTAAAC TGTCACCCAC GTATGTTTTG
    TATATGACAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CTGACATCAC TCCTACTGAG
    CGTACTCACA AAGTTATTGC AATAGTCAAC AAGATGGTGA ATATGATGGA AGGAGTCATA
    CAGGAGGTAG ACCAGGTTGA TCAGAAACAG AAGAATATTG CTGGGGCACT TGCCTTTTGG
    ATGGCAAATG CGTCTGAATT GCTGAATTTT ATAAAGCAAG ACCGTGATCT GAGCCGAATC
    ACTGTGGATG CTCAAGATGT TTTGGCGCAC TTGGTTCAAA TGGCATTCAA GTACCTAGTT
    CATTGTCTGC AGTCAGAGCT TAATAACTAC ATGCCAGCAT TTCTTGATGA TCCAGAGGAA
    AATAATCCAC AGAGGCCTAA AATAGATGAT GTGCTGCACA CATTGACTGG AGCTATGTCC
    TTGTTGCGAC GATGTAGAGT GAATGCTGCA CTGACTATAC AGCTCTTCTC CCAGTTGTTT
    CATTTTATCA ACATGTGGCT TTTTAACAGA CTAGTTACGG CTCCAGATTC AGGGTTATGT
    TCCCACTACT GGGGAGCTAT TGTTCGTCAG CAGTTGGGTC ATATTGAGGC CTGGGCAGAA
    AAACAGGGTT TAGAATTGGC TGCTGATTGC CACTTAAGCA GAATAGTGCA GGCAACCACA
    TTGCTCACCA TGGACAAGTA CTCACATGCT GATGTTCCAA ATATTAACAG TACTTGCTTT
    AAGTTGAATT CTCTACAGCT ACAAGCTTTG TTGCAGAATT ATCACTGTGC TCCAGATGAA
    CCGCTTATAC CAACAGAACT GATAGAGAAT GTAGTAACTG TTGCAGAAAA TACGGCTGAT
    GAACTTGCTC GCAGTGATGG CAGAGAAGTC CAATTGGAAG AGGATCCTGA CTTACAACTC
    CCTTTTCTTT TACCAGAAGA TGGCTATTCC TGTGATGTTG TCAGAAATGT TCCAAGTGGT
    TTACAAGAAT TTTTGGATCC CCTCTGTCAG AGAGGTTTTT GTAGGTTAAT ACCTCATACA
    CGGTCACCAG GCACATGGAC AATATACTTT GAAGGCGCAG ACTATGAAAG CCACCTGTCA
    CATGAGAATG CTGAGCTAGC TCAGCCTTTG AGGAAAGAAC CTGAGATTAT AACTGTAACA
    CTAAAAAAAC AAAATGGAAT GGGCCTCAGC ATTGTTGCTG CCAAGGGTGC TGGTCAAGAC
    AAACTTGGAA TATATGTCAA GTCTGTTGTG AAAGGAGGTG CTGCAGATGT GGATGGTCGT
    CTGGCAGCAG GGGATCAGCT GCTCAGTGTG GATGGTCGAA GCTTGGTGGG GCTGTCCCAG
    GAAAGGGCAG CAGAACTTAT GACGAGGACA GGTTCTGTTG TAACACTGGA AGTAGCAAAA
    CAAGGAGCAA TATACCACGG TCTGGCAACC CTGCTAAATC AGCCATCTCC AATGATGCAA
    AGAGTTTCTG ACCGCCGTGG CTCAGGTAAA CCCCGACCAA AGAGTGAAGG TTTTGAGCTG
    TATAACAACT CCACGCAAAA TGGGTCACCT GAGAGCCCCC AGCTGCCATG GACGGAGTAC
    AGTGAACCAA AGAAGCTGCC AGGAGATGAC AGATTAATGA AGAACAGAGC CGATCATCGC
    TCCAGCCCCA ACGTAGCAAA TCAGCCTCCA AGCCCTGGGG GGAAGAATGC TTATGCATCT
    GGAACTACCA CCAAAATAAC CTCAGTCTCG ACTGGAAATC TTTGTACAGA GGAGCAAACT
    CTGCCTCCAC GACCTGAAGC TTATCCTATC CCAACACAGA CCTATACCAG AGAATATTTC
    ACATTCCCAG CTTCAAAATC CCAGGATCGA ATGGGTCCAG CTCAGAGTCA GTGGCCAAAT
    TATGAAGAGA AGCCACAGAT CCAGGCAGAA AGTAATCACT CAATCAATAC TACAATGCAG
    CGCGTGGCTC GATCCCAGGA AGAACTCCGA GATAAAGTTT TCCAGCCAGA GAGGAATCGT
    TTGGAAGTTG TTATCCGGAA GGACATAGAA TACATTGATC GGAAGTCAGA CAGCGATCAG
    TGGATGAATT CATCTGTGGA TTCTAGCACA TCTAGCCAAG AGCACCTGAA CCATACATCC
    AAACCTGTTT CACCTGGTTC TTGCTTAACT AAAAGTGGAC CTGGCCGTTG GAAAACTCCA
    GCTACGAGCC AGCCAACTCC AGTGGCCGTT TCCCAGCCCA TCAGAACAGA CCTTCCTCCT
    CCGCCGCCAC CTCCTCCGGT GCATTATGCA GCTGAATTTG ATGAACCACA AATGGATTTG
    CCTCTGCCTC CCCCTCCTCC ACCGAACCAA GTAGGAGCAC AAGCATCTTC TCAGGTGGCT
    GCTGCTGAGC GTAAAAAACG AGAAGAGCAT CAGAGGTGGT ATGAAAAGGA AAAGGCACGT
    TTGGAAGAAG AACGAGAAAG GAAACGCCGT GAGCAAGAGA GAAAACTGGG CCAAATGCGT
    TCGCAGCCAC TAATTCCTCC CCAGCCTGTG GTTCCTCCTG TTTCCCTTCA GCAAGTGAAA
    TCGGAAAAAA CTTCAACTTT CCAGAGGTCT CAAGAAACAG TCATTCGAGA GTTGCAGCCG
    CAACAGCAGC CTCGGACTAT TGAGCGGAGA GATCTGCAGT ACATTACTAT CAGCAAGGAA
    GAATTGTCCT CTAGTGACAG CCTCTCTCCA GATCCCTGGA AACGAGATGC CAAGGAGAAA
    CTGGAGAAGC AGCAGCAGAT GCACATTGTG GACATGCTGA GCAAAGAGAT TCAGGATCTT
    CAGAACAAGC CTGATCGTAC AGCTGAAGAG AACGACCGCC TGCGCAAACT CATGCTAGAG
    TGGCAGTTCC AAAAGCGACT TCAGGAATCA AAACAAAAGG ATGAAGATGA TGATGAAGAA
    GAGGACGATG ATGTGGATAC TATGCTTATC ATGCAGCGGC TGGAGGCAGA AAGAAGAGCA
    AGGCAGACTG CTGTGCCAGC AATCTCTGTT CTAGACTTGT TACAGGATGA AGAACGCAGA
    CGACAGCAAC AGTTAGAAGA AATGCGCAAG CGAGAAGCAG AAGACAGGGC CAGGCAGGAA
    GAGGAGCGCC GACAGCAAGA GGAGGAGCGA ACCAGGAGAG AGGCAGAAGA AAAGAGGCGA
    CAGGAAGAGG AGTATTACAA TCGCTTAGAG GCTGAAAGGC GCAGGCAGCA CGATGAAGCA
    GAACGAAGAT TGCTGGAACC AGACGAGCCT GGTCCTCTCC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33810
    Clone ID Related Accession (Same CDS sequence) XM_015284360.1 , XM_015284360.2
    Accession Version XM_015284360.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product afadin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAAGAA GATTAGATGT GGATGAGAAG CCTCTTGTTG TACAATTAAA CTGGAACAAA
    GATGATCGAG AAGGAAGGTT TGTTCTCAAG AATGAAAATG ATACGCTTCC TCCAAAGAAG
    GCTCAGAGTA ATGGCCCTGA AAAACAAGAA AAAGAAGGAG TAATACAGAA TTTCAAACGA
    ACTCTCTCAA AGAAAGAAAA GAAAGAGAAG AAGAGGCGTG AAAAAGAGGC ATTGCGACAA
    GCATCAGATA AAGATGATCG ACTTCTTCAT GGGGATGATA TTGAGAATTC TCGCCTTGCA
    GCTGAGGTTT ACAAAGATAT GCCTGAGACC AGCTTCACTC GCACAATATC TAATCCTGAG
    GTGGTTATGA AACGACGGCG GCAGCAAAAA CTAGAGAAGA GAATGCAGGA ATTTCGCAGT
    TCTGATGGCA GACCAGACTC AGGTGGGACG CTGAGAATTT ATGCGGACAG TTTAAAACCA
    AATATACCAT ACAAGACAAT CCTGTTGTCA ACTACAGATA CTGCAGACTT TGCAGTTATT
    GAAGCACTGG AGAAATATGG CTTGGAGAAG GAAAACCCCA AGGATTATTG TATTGCTCGT
    GTCATTCTGC CTCCTGGTGC TCAGCACTCT GATGATAAAG GTGCTAAAGA AACTATTCTA
    GATGATGATG AGTGTCCACT TCAGATATTC AGGGAATGGC CTAGTGATAA AGGAATACTA
    GTCTTTCAGT TGAAAAGGCG GCCTCCTGAT TATGTCCCAA AGAAATCCAA GAAAATAGCT
    GATGGAAAGC CATTAAAGGG GAAGGAAAGA GTTGACGGGT CTGGCTACGG CTCTTGCCTT
    CCTCCTGAGA AACTACCATA CCTGGTAGAA TTAAGCCCAG ATGGTTCTGA CTCCAGAGAT
    AAACCAAAGC TTTATCGTCT ACAATTAAGT GTCACTGAAG TTGGAACTGA AAAATTTGAT
    GACAATTCCA TTCAGTTATT TGGTCCAGGA ATTCAGCCTC ATCATTGTGA TCTTACTAAT
    ATGGATGGAG TTGTTACTGT AACCCCAAGA AACATTGATG CGGAAACGTA TGTAGAAGGT
    CAGCGTATTT CAGAAACCAC TATGCTGCAA AGTGGAATGA AGGTTCAGTT TGGGTCATCT
    CATGTGTTTA AGTTTGTGGA TCCCACTCAG GACCATATTA TTACTAAAAG ACCTATTGAT
    GCAAGCCTTA TGGTCAAAGG TCCAAGGCAT AAAGCTGGAG CTGTTCAGGA AACCACGTTT
    GATCTGGAGG GAGATGTTCA CAGTGGAACA GCGTTACCAG CGAGCAAGAG CACCAGCAGG
    CTTGATGGTG ACAGAACATC ATCAGCATCC AGCACAGCTG AACGAGGAAT GGTGAAGCCC
    ATGATTAGAA TAGAACAGCA GCAAGATTAT CGCAGACAGG AAAGCAGACC TCAGGATACC
    CATGGACCAG AACTGATCCT ACCTGCCAGT ATTGAATTCA GGGAAAGCTC TGAAGATGCC
    TTCTTATCTG CCATTATAAA TTATACAAAT AGCTCGACAG TTCATTTTAA ACTGTCACCC
    ACGTATGTTT TGTATATGAC ATGTCGGTAT GTATTGTCAA GCCAGTACAG ACCTGACATC
    ACTCCTACTG AGCGTACTCA CAAAGTTATT GCAATAGTCA ACAAGATGGT GAATATGATG
    GAAGGAGTCA TACAGAAACA GAAGAATATT GCTGGGGCAC TTGCCTTTTG GATGGCAAAT
    GCGTCTGAAT TGCTGAATTT TATAAAGCAA GACCGTGATC TGAGCCGAAT CACTGTGGAT
    GCTCAAGATG TTTTGGCGCA CTTGGTTCAA ATGGCATTCA AGTACCTAGT TCATTGTCTG
    CAGTCAGAGC TTAATAACTA CATGCCAGCA TTTCTTGATG ATCCAGAGGA AAATAATCCA
    CAGAGGCCTA AAATAGATGA TGTGCTGCAC ACATTGACTG GAGCTATGTC CTTGTTGCGA
    CGATGTAGAG TGAATGCTGC ACTGACTATA CAGCTCTTCT CCCAGTTGTT TCATTTTATC
    AACATGTGGC TTTTTAACAG ACTAGTTACG GCTCCAGATT CAGGGTTATG TTCCCACTAC
    TGGGGAGCTA TTGTTCGTCA GCAGTTGGGT CATATTGAGG CCTGGGCAGA AAAACAGGGT
    TTAGAATTGG CTGCTGATTG CCACTTAAGC AGAATAGTGC AGGCAACCAC ATTGCTCACC
    ATGGACAAGT ACTCACATGC TGATGTTCCA AATATTAACA GTACTTGCTT TAAGTTGAAT
    TCTCTACAGC TACAAGCTTT GTTGCAGAAT TATCACTGTG CTCCAGATGA ACCGCTTATA
    CCAACAGAAC TGATAGAGAA TGTAGTAACT GTTGCAGAAA ATACGGCTGA TGAACTTGCT
    CGCAGTGATG GCAGAGAAGT CCAATTGGAA GAGGATCCTG ACTTACAACT CCCTTTTCTT
    TTACCAGAAG ATGGCTATTC CTGTGATGTT GTCAGAAATG TTCCAAGTGG TTTACAAGAA
    TTTTTGGATC CCCTCTGTCA GAGAGGTTTT TGTAGGTTAA TACCTCATAC ACGGTCACCA
    GGCACATGGA CAATATACTT TGAAGGCGCA GACTATGAAA GCCACCTGTC ACATGAGAAT
    GCTGAGCTAG CTCAGCCTTT GAGGAAAGAA CCTGAGATTA TAACTGTAAC ACTAAAAAAA
    CAAAATGGAA TGGGCCTCAG CATTGTTGCT GCCAAGGGTG CTGGTCAAGA CAAACTTGGA
    ATATATGTCA AGTCTGTTGT GAAAGGAGGT GCTGCAGATG TGGATGGTCG TCTGGCAGCA
    GGGGATCAGC TGCTCAGTGT GGATGGTCGA AGCTTGGTGG GGCTGTCCCA GGAAAGGGCA
    GCAGAACTTA TGACGAGGAC AGGTTCTGTT GTAACACTGG AAGTAGCAAA ACAAGGAGCA
    ATATACCACG GTCTGGCAAC CCTGCTAAAT CAGCCATCTC CAATGATGCA AAGAGTTTCT
    GACCGCCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTTGAGCT GTATAACAAC
    TCCACGCAAA ATGGGTCACC TGAGAGCCCC CAGCTGCCAT GGACGGAGTA CAGTGAACCA
    AAGAAGCTGC CAGGAGATGA CAGATTAATG AAGAACAGAG CCGATCATCG CTCCAGCCCC
    AACGTAGCAA ATCAGCCTCC AAGCCCTGGG GGGAAGAATG CTTATGCATC TGGAACTACC
    ACCAAAATAA CCTCAGTCTC GACTGGAAAT CTTTGTACAG AGGAGCAAAC TCTGCCTCCA
    CGACCTGAAG CTTATCCTAT CCCAACACAG ACCTATACCA GAGAATATTT CACATTCCCA
    GCTTCAAAAT CCCAGGATCG AATGGGTCCA GCTCAGAGTC AGTGGCCAAA TTATGAAGAG
    AAGCCACAGA TCCAGGCAGA AAGTAATCAC TCAATCAATA CTACAATGCA GCGCGTGGCT
    CGATCCCAGG AAGAACTCCG AGATAAAGTT TTCCAGCCAG AGAGGAATCG TTTGGAAGTT
    GTTATCCGGA AGGACATAGA ATACATTGAT CGGAAGTCAG ACAGCGATCA GTGGATGAAT
    TCATCTGTGG ATTCTAGCAC ATCTAGCCAA GAGCACCTGA ACCATACATC CAAACCTGTT
    TCACCTGGTT CTTGCTTAAC TAAAAGTGGA CCTGGCCGTT GGAAAACTCC AGCTACGAGC
    CAGCCAACTC CAGTGGCCGT TTCCCAGCCC ATCAGAACAG ACCTTCCTCC TCCGCCGCCA
    CCTCCTCCGG TGCATTATGC AGCTGAATTT GATGAACCAC AAATGGATTT GCCTCTGCCT
    CCCCCTCCTC CACCGAACCA AGTAGGAGCA CAAGCATCTT CTCAGGTGGC TGCTGCTGAG
    CGTAAAAAAC GAGAAGAGCA TCAGAGGTGG TATGAAAAGG AAAAGGCACG TTTGGAAGAA
    GAACGAGAAA GGAAACGCCG TGAGCAAGAG AGAAAACTGG GCCAAATGCG TTCGCAGCCA
    CTAATTCCTC CCCAGCCTGT GGTTCCTCCT GTTTCCCTTC AGCAAGTGAA ATCGGAAAAA
    ACTTCAACTT TCCAGAGGTC TCAAGAAACA GTCATTCGAG AGTTGCAGCC GCAACAGCAG
    CCTCGGACTA TTGAGCGGAG AGATCTGCAG TACATTACTA TCAGCAAGGA AGAATTGTCC
    TCTAGTGACA GCCTCTCTCC AGATCCCTGG AAACGAGATG CCAAGGAGAA ACTGGAGAAG
    CAGCAGCAGA TGCACATTGT GGACATGCTG AGCAAAGAGA TTCAGGATCT TCAGAACAAG
    CCTGATCGTA CAGCTGAAGA GAACGACCGC CTGCGCAAAC TCATGCTAGA GTGGCAGTTC
    CAAAAGCGAC TTCAGGAATC AAAACAAAAG GATGAAGATG ATGATGAAGA AGAGGACGAT
    GATGTGGATA CTATGCTTAT CATGCAGCGG CTGGAGGCAG AAAGAAGAGC AAGGTTACAG
    GATGAAGAAC GCAGACGACA GCAACAGTTA GAAGAAATGC GCAAGCGAGA AGCAGAAGAC
    AGGGCCAGGC AGGAAGAGGA GCGCCGACAG CAAGAGGAGG AGCGAACCAG GAGAGAGGCA
    GAAGAAAAGA GGCGACAGGA AGAGGAGTAT TACAATCGCT TAGAGGCTGA AAGGCGCAGG
    CAGCACGATG AAGCAGAACG AAGATTGCTG GAACCAGACG AGCCTGGAAT AAATTCATAC
    ACTGGATCTT CGGGAGCAAC GGTTGGGGCC CATGAAGTCT ATCGGGATCC AAGAGACAAA
    CGATCTAAAA GTCAAGATAC AGATTTACCT GGAGCACCAG AAAATCTGAC GTTCAGGGAA
    CGCCAGCGAC TTTTTTCACA GGGTCAAGAT GTATCCAATA AAGTAAAAGC CTCTAGGAAA
    CTAATGGAAC TGGAAAATGA GTTGAACACG AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139846.1
    CDS68..5323
    Translation

    Target ORF information:

    RefSeq Version XM_015284360.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284360.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAAGAA GATTAGATGT GGATGAGAAG CCTCTTGTTG TACAATTAAA CTGGAACAAA
    GATGATCGAG AAGGAAGGTT TGTTCTCAAG AATGAAAATG ATACGCTTCC TCCAAAGAAG
    GCTCAGAGTA ATGGCCCTGA AAAACAAGAA AAAGAAGGAG TAATACAGAA TTTCAAACGA
    ACTCTCTCAA AGAAAGAAAA GAAAGAGAAG AAGAGGCGTG AAAAAGAGGC ATTGCGACAA
    GCATCAGATA AAGATGATCG ACTTCTTCAT GGGGATGATA TTGAGAATTC TCGCCTTGCA
    GCTGAGGTTT ACAAAGATAT GCCTGAGACC AGCTTCACTC GCACAATATC TAATCCTGAG
    GTGGTTATGA AACGACGGCG GCAGCAAAAA CTAGAGAAGA GAATGCAGGA ATTTCGCAGT
    TCTGATGGCA GACCAGACTC AGGTGGGACG CTGAGAATTT ATGCGGACAG TTTAAAACCA
    AATATACCAT ACAAGACAAT CCTGTTGTCA ACTACAGATA CTGCAGACTT TGCAGTTATT
    GAAGCACTGG AGAAATATGG CTTGGAGAAG GAAAACCCCA AGGATTATTG TATTGCTCGT
    GTCATTCTGC CTCCTGGTGC TCAGCACTCT GATGATAAAG GTGCTAAAGA AACTATTCTA
    GATGATGATG AGTGTCCACT TCAGATATTC AGGGAATGGC CTAGTGATAA AGGAATACTA
    GTCTTTCAGT TGAAAAGGCG GCCTCCTGAT TATGTCCCAA AGAAATCCAA GAAAATAGCT
    GATGGAAAGC CATTAAAGGG GAAGGAAAGA GTTGACGGGT CTGGCTACGG CTCTTGCCTT
    CCTCCTGAGA AACTACCATA CCTGGTAGAA TTAAGCCCAG ATGGTTCTGA CTCCAGAGAT
    AAACCAAAGC TTTATCGTCT ACAATTAAGT GTCACTGAAG TTGGAACTGA AAAATTTGAT
    GACAATTCCA TTCAGTTATT TGGTCCAGGA ATTCAGCCTC ATCATTGTGA TCTTACTAAT
    ATGGATGGAG TTGTTACTGT AACCCCAAGA AACATTGATG CGGAAACGTA TGTAGAAGGT
    CAGCGTATTT CAGAAACCAC TATGCTGCAA AGTGGAATGA AGGTTCAGTT TGGGTCATCT
    CATGTGTTTA AGTTTGTGGA TCCCACTCAG GACCATATTA TTACTAAAAG ACCTATTGAT
    GCAAGCCTTA TGGTCAAAGG TCCAAGGCAT AAAGCTGGAG CTGTTCAGGA AACCACGTTT
    GATCTGGAGG GAGATGTTCA CAGTGGAACA GCGTTACCAG CGAGCAAGAG CACCAGCAGG
    CTTGATGGTG ACAGAACATC ATCAGCATCC AGCACAGCTG AACGAGGAAT GGTGAAGCCC
    ATGATTAGAA TAGAACAGCA GCAAGATTAT CGCAGACAGG AAAGCAGACC TCAGGATACC
    CATGGACCAG AACTGATCCT ACCTGCCAGT ATTGAATTCA GGGAAAGCTC TGAAGATGCC
    TTCTTATCTG CCATTATAAA TTATACAAAT AGCTCGACAG TTCATTTTAA ACTGTCACCC
    ACGTATGTTT TGTATATGAC ATGTCGGTAT GTATTGTCAA GCCAGTACAG ACCTGACATC
    ACTCCTACTG AGCGTACTCA CAAAGTTATT GCAATAGTCA ACAAGATGGT GAATATGATG
    GAAGGAGTCA TACAGAAACA GAAGAATATT GCTGGGGCAC TTGCCTTTTG GATGGCAAAT
    GCGTCTGAAT TGCTGAATTT TATAAAGCAA GACCGTGATC TGAGCCGAAT CACTGTGGAT
    GCTCAAGATG TTTTGGCGCA CTTGGTTCAA ATGGCATTCA AGTACCTAGT TCATTGTCTG
    CAGTCAGAGC TTAATAACTA CATGCCAGCA TTTCTTGATG ATCCAGAGGA AAATAATCCA
    CAGAGGCCTA AAATAGATGA TGTGCTGCAC ACATTGACTG GAGCTATGTC CTTGTTGCGA
    CGATGTAGAG TGAATGCTGC ACTGACTATA CAGCTCTTCT CCCAGTTGTT TCATTTTATC
    AACATGTGGC TTTTTAACAG ACTAGTTACG GCTCCAGATT CAGGGTTATG TTCCCACTAC
    TGGGGAGCTA TTGTTCGTCA GCAGTTGGGT CATATTGAGG CCTGGGCAGA AAAACAGGGT
    TTAGAATTGG CTGCTGATTG CCACTTAAGC AGAATAGTGC AGGCAACCAC ATTGCTCACC
    ATGGACAAGT ACTCACATGC TGATGTTCCA AATATTAACA GTACTTGCTT TAAGTTGAAT
    TCTCTACAGC TACAAGCTTT GTTGCAGAAT TATCACTGTG CTCCAGATGA ACCGCTTATA
    CCAACAGAAC TGATAGAGAA TGTAGTAACT GTTGCAGAAA ATACGGCTGA TGAACTTGCT
    CGCAGTGATG GCAGAGAAGT CCAATTGGAA GAGGATCCTG ACTTACAACT CCCTTTTCTT
    TTACCAGAAG ATGGCTATTC CTGTGATGTT GTCAGAAATG TTCCAAGTGG TTTACAAGAA
    TTTTTGGATC CCCTCTGTCA GAGAGGTTTT TGTAGGTTAA TACCTCATAC ACGGTCACCA
    GGCACATGGA CAATATACTT TGAAGGCGCA GACTATGAAA GCCACCTGTC ACATGAGAAT
    GCTGAGCTAG CTCAGCCTTT GAGGAAAGAA CCTGAGATTA TAACTGTAAC ACTAAAAAAA
    CAAAATGGAA TGGGCCTCAG CATTGTTGCT GCCAAGGGTG CTGGTCAAGA CAAACTTGGA
    ATATATGTCA AGTCTGTTGT GAAAGGAGGT GCTGCAGATG TGGATGGTCG TCTGGCAGCA
    GGGGATCAGC TGCTCAGTGT GGATGGTCGA AGCTTGGTGG GGCTGTCCCA GGAAAGGGCA
    GCAGAACTTA TGACGAGGAC AGGTTCTGTT GTAACACTGG AAGTAGCAAA ACAAGGAGCA
    ATATACCACG GTCTGGCAAC CCTGCTAAAT CAGCCATCTC CAATGATGCA AAGAGTTTCT
    GACCGCCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTTGAGCT GTATAACAAC
    TCCACGCAAA ATGGGTCACC TGAGAGCCCC CAGCTGCCAT GGACGGAGTA CAGTGAACCA
    AAGAAGCTGC CAGGAGATGA CAGATTAATG AAGAACAGAG CCGATCATCG CTCCAGCCCC
    AACGTAGCAA ATCAGCCTCC AAGCCCTGGG GGGAAGAATG CTTATGCATC TGGAACTACC
    ACCAAAATAA CCTCAGTCTC GACTGGAAAT CTTTGTACAG AGGAGCAAAC TCTGCCTCCA
    CGACCTGAAG CTTATCCTAT CCCAACACAG ACCTATACCA GAGAATATTT CACATTCCCA
    GCTTCAAAAT CCCAGGATCG AATGGGTCCA GCTCAGAGTC AGTGGCCAAA TTATGAAGAG
    AAGCCACAGA TCCAGGCAGA AAGTAATCAC TCAATCAATA CTACAATGCA GCGCGTGGCT
    CGATCCCAGG AAGAACTCCG AGATAAAGTT TTCCAGCCAG AGAGGAATCG TTTGGAAGTT
    GTTATCCGGA AGGACATAGA ATACATTGAT CGGAAGTCAG ACAGCGATCA GTGGATGAAT
    TCATCTGTGG ATTCTAGCAC ATCTAGCCAA GAGCACCTGA ACCATACATC CAAACCTGTT
    TCACCTGGTT CTTGCTTAAC TAAAAGTGGA CCTGGCCGTT GGAAAACTCC AGCTACGAGC
    CAGCCAACTC CAGTGGCCGT TTCCCAGCCC ATCAGAACAG ACCTTCCTCC TCCGCCGCCA
    CCTCCTCCGG TGCATTATGC AGCTGAATTT GATGAACCAC AAATGGATTT GCCTCTGCCT
    CCCCCTCCTC CACCGAACCA AGTAGGAGCA CAAGCATCTT CTCAGGTGGC TGCTGCTGAG
    CGTAAAAAAC GAGAAGAGCA TCAGAGGTGG TATGAAAAGG AAAAGGCACG TTTGGAAGAA
    GAACGAGAAA GGAAACGCCG TGAGCAAGAG AGAAAACTGG GCCAAATGCG TTCGCAGCCA
    CTAATTCCTC CCCAGCCTGT GGTTCCTCCT GTTTCCCTTC AGCAAGTGAA ATCGGAAAAA
    ACTTCAACTT TCCAGAGGTC TCAAGAAACA GTCATTCGAG AGTTGCAGCC GCAACAGCAG
    CCTCGGACTA TTGAGCGGAG AGATCTGCAG TACATTACTA TCAGCAAGGA AGAATTGTCC
    TCTAGTGACA GCCTCTCTCC AGATCCCTGG AAACGAGATG CCAAGGAGAA ACTGGAGAAG
    CAGCAGCAGA TGCACATTGT GGACATGCTG AGCAAAGAGA TTCAGGATCT TCAGAACAAG
    CCTGATCGTA CAGCTGAAGA GAACGACCGC CTGCGCAAAC TCATGCTAGA GTGGCAGTTC
    CAAAAGCGAC TTCAGGAATC AAAACAAAAG GATGAAGATG ATGATGAAGA AGAGGACGAT
    GATGTGGATA CTATGCTTAT CATGCAGCGG CTGGAGGCAG AAAGAAGAGC AAGGTTACAG
    GATGAAGAAC GCAGACGACA GCAACAGTTA GAAGAAATGC GCAAGCGAGA AGCAGAAGAC
    AGGGCCAGGC AGGAAGAGGA GCGCCGACAG CAAGAGGAGG AGCGAACCAG GAGAGAGGCA
    GAAGAAAAGA GGCGACAGGA AGAGGAGTAT TACAATCGCT TAGAGGCTGA AAGGCGCAGG
    CAGCACGATG AAGCAGAACG AAGATTGCTG GAACCAGACG AGCCTGGAAT AAATTCATAC
    ACTGGATCTT CGGGAGCAAC GGTTGGGGCC CATGAAGTCT ATCGGGATCC AAGAGACAAA
    CGATCTAAAA GTCAAGATAC AGATTTACCT GGAGCACCAG AAAATCTGAC GTTCAGGGAA
    CGCCAGCGAC TTTTTTCACA GGGTCAAGAT GTATCCAATA AAGTAAAAGC CTCTAGGAAA
    CTAATGGAAC TGGAAAATGA GTTGAACACG AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33810
    Clone ID Related Accession (Same CDS sequence) XM_015284360.1 , XM_015284360.2
    Accession Version XM_015284360.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product afadin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAAGAA GATTAGATGT GGATGAGAAG CCTCTTGTTG TACAATTAAA CTGGAACAAA
    GATGATCGAG AAGGAAGGTT TGTTCTCAAG AATGAAAATG ATACGCTTCC TCCAAAGAAG
    GCTCAGAGTA ATGGCCCTGA AAAACAAGAA AAAGAAGGAG TAATACAGAA TTTCAAACGA
    ACTCTCTCAA AGAAAGAAAA GAAAGAGAAG AAGAGGCGTG AAAAAGAGGC ATTGCGACAA
    GCATCAGATA AAGATGATCG ACTTCTTCAT GGGGATGATA TTGAGAATTC TCGCCTTGCA
    GCTGAGGTTT ACAAAGATAT GCCTGAGACC AGCTTCACTC GCACAATATC TAATCCTGAG
    GTGGTTATGA AACGACGGCG GCAGCAAAAA CTAGAGAAGA GAATGCAGGA ATTTCGCAGT
    TCTGATGGCA GACCAGACTC AGGTGGGACG CTGAGAATTT ATGCGGACAG TTTAAAACCA
    AATATACCAT ACAAGACAAT CCTGTTGTCA ACTACAGATA CTGCAGACTT TGCAGTTATT
    GAAGCACTGG AGAAATATGG CTTGGAGAAG GAAAACCCCA AGGATTATTG TATTGCTCGT
    GTCATTCTGC CTCCTGGTGC TCAGCACTCT GATGATAAAG GTGCTAAAGA AACTATTCTA
    GATGATGATG AGTGTCCACT TCAGATATTC AGGGAATGGC CTAGTGATAA AGGAATACTA
    GTCTTTCAGT TGAAAAGGCG GCCTCCTGAT TATGTCCCAA AGAAATCCAA GAAAATAGCT
    GATGGAAAGC CATTAAAGGG GAAGGAAAGA GTTGACGGGT CTGGCTACGG CTCTTGCCTT
    CCTCCTGAGA AACTACCATA CCTGGTAGAA TTAAGCCCAG ATGGTTCTGA CTCCAGAGAT
    AAACCAAAGC TTTATCGTCT ACAATTAAGT GTCACTGAAG TTGGAACTGA AAAATTTGAT
    GACAATTCCA TTCAGTTATT TGGTCCAGGA ATTCAGCCTC ATCATTGTGA TCTTACTAAT
    ATGGATGGAG TTGTTACTGT AACCCCAAGA AACATTGATG CGGAAACGTA TGTAGAAGGT
    CAGCGTATTT CAGAAACCAC TATGCTGCAA AGTGGAATGA AGGTTCAGTT TGGGTCATCT
    CATGTGTTTA AGTTTGTGGA TCCCACTCAG GACCATATTA TTACTAAAAG ACCTATTGAT
    GCAAGCCTTA TGGTCAAAGG TCCAAGGCAT AAAGCTGGAG CTGTTCAGGA AACCACGTTT
    GATCTGGAGG GAGATGTTCA CAGTGGAACA GCGTTACCAG CGAGCAAGAG CACCAGCAGG
    CTTGATGGTG ACAGAACATC ATCAGCATCC AGCACAGCTG AACGAGGAAT GGTGAAGCCC
    ATGATTAGAA TAGAACAGCA GCAAGATTAT CGCAGACAGG AAAGCAGACC TCAGGATACC
    CATGGACCAG AACTGATCCT ACCTGCCAGT ATTGAATTCA GGGAAAGCTC TGAAGATGCC
    TTCTTATCTG CCATTATAAA TTATACAAAT AGCTCGACAG TTCATTTTAA ACTGTCACCC
    ACGTATGTTT TGTATATGAC ATGTCGGTAT GTATTGTCAA GCCAGTACAG ACCTGACATC
    ACTCCTACTG AGCGTACTCA CAAAGTTATT GCAATAGTCA ACAAGATGGT GAATATGATG
    GAAGGAGTCA TACAGAAACA GAAGAATATT GCTGGGGCAC TTGCCTTTTG GATGGCAAAT
    GCGTCTGAAT TGCTGAATTT TATAAAGCAA GACCGTGATC TGAGCCGAAT CACTGTGGAT
    GCTCAAGATG TTTTGGCGCA CTTGGTTCAA ATGGCATTCA AGTACCTAGT TCATTGTCTG
    CAGTCAGAGC TTAATAACTA CATGCCAGCA TTTCTTGATG ATCCAGAGGA AAATAATCCA
    CAGAGGCCTA AAATAGATGA TGTGCTGCAC ACATTGACTG GAGCTATGTC CTTGTTGCGA
    CGATGTAGAG TGAATGCTGC ACTGACTATA CAGCTCTTCT CCCAGTTGTT TCATTTTATC
    AACATGTGGC TTTTTAACAG ACTAGTTACG GCTCCAGATT CAGGGTTATG TTCCCACTAC
    TGGGGAGCTA TTGTTCGTCA GCAGTTGGGT CATATTGAGG CCTGGGCAGA AAAACAGGGT
    TTAGAATTGG CTGCTGATTG CCACTTAAGC AGAATAGTGC AGGCAACCAC ATTGCTCACC
    ATGGACAAGT ACTCACATGC TGATGTTCCA AATATTAACA GTACTTGCTT TAAGTTGAAT
    TCTCTACAGC TACAAGCTTT GTTGCAGAAT TATCACTGTG CTCCAGATGA ACCGCTTATA
    CCAACAGAAC TGATAGAGAA TGTAGTAACT GTTGCAGAAA ATACGGCTGA TGAACTTGCT
    CGCAGTGATG GCAGAGAAGT CCAATTGGAA GAGGATCCTG ACTTACAACT CCCTTTTCTT
    TTACCAGAAG ATGGCTATTC CTGTGATGTT GTCAGAAATG TTCCAAGTGG TTTACAAGAA
    TTTTTGGATC CCCTCTGTCA GAGAGGTTTT TGTAGGTTAA TACCTCATAC ACGGTCACCA
    GGCACATGGA CAATATACTT TGAAGGCGCA GACTATGAAA GCCACCTGTC ACATGAGAAT
    GCTGAGCTAG CTCAGCCTTT GAGGAAAGAA CCTGAGATTA TAACTGTAAC ACTAAAAAAA
    CAAAATGGAA TGGGCCTCAG CATTGTTGCT GCCAAGGGTG CTGGTCAAGA CAAACTTGGA
    ATATATGTCA AGTCTGTTGT GAAAGGAGGT GCTGCAGATG TGGATGGTCG TCTGGCAGCA
    GGGGATCAGC TGCTCAGTGT GGATGGTCGA AGCTTGGTGG GGCTGTCCCA GGAAAGGGCA
    GCAGAACTTA TGACGAGGAC AGGTTCTGTT GTAACACTGG AAGTAGCAAA ACAAGGAGCA
    ATATACCACG GTCTGGCAAC CCTGCTAAAT CAGCCATCTC CAATGATGCA AAGAGTTTCT
    GACCGCCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTTGAGCT GTATAACAAC
    TCCACGCAAA ATGGGTCACC TGAGAGCCCC CAGCTGCCAT GGACGGAGTA CAGTGAACCA
    AAGAAGCTGC CAGGAGATGA CAGATTAATG AAGAACAGAG CCGATCATCG CTCCAGCCCC
    AACGTAGCAA ATCAGCCTCC AAGCCCTGGG GGGAAGAATG CTTATGCATC TGGAACTACC
    ACCAAAATAA CCTCAGTCTC GACTGGAAAT CTTTGTACAG AGGAGCAAAC TCTGCCTCCA
    CGACCTGAAG CTTATCCTAT CCCAACACAG ACCTATACCA GAGAATATTT CACATTCCCA
    GCTTCAAAAT CCCAGGATCG AATGGGTCCA GCTCAGAGTC AGTGGCCAAA TTATGAAGAG
    AAGCCACAGA TCCAGGCAGA AAGTAATCAC TCAATCAATA CTACAATGCA GCGCGTGGCT
    CGATCCCAGG AAGAACTCCG AGATAAAGTT TTCCAGCCAG AGAGGAATCG TTTGGAAGTT
    GTTATCCGGA AGGACATAGA ATACATTGAT CGGAAGTCAG ACAGCGATCA GTGGATGAAT
    TCATCTGTGG ATTCTAGCAC ATCTAGCCAA GAGCACCTGA ACCATACATC CAAACCTGTT
    TCACCTGGTT CTTGCTTAAC TAAAAGTGGA CCTGGCCGTT GGAAAACTCC AGCTACGAGC
    CAGCCAACTC CAGTGGCCGT TTCCCAGCCC ATCAGAACAG ACCTTCCTCC TCCGCCGCCA
    CCTCCTCCGG TGCATTATGC AGCTGAATTT GATGAACCAC AAATGGATTT GCCTCTGCCT
    CCCCCTCCTC CACCGAACCA AGTAGGAGCA CAAGCATCTT CTCAGGTGGC TGCTGCTGAG
    CGTAAAAAAC GAGAAGAGCA TCAGAGGTGG TATGAAAAGG AAAAGGCACG TTTGGAAGAA
    GAACGAGAAA GGAAACGCCG TGAGCAAGAG AGAAAACTGG GCCAAATGCG TTCGCAGCCA
    CTAATTCCTC CCCAGCCTGT GGTTCCTCCT GTTTCCCTTC AGCAAGTGAA ATCGGAAAAA
    ACTTCAACTT TCCAGAGGTC TCAAGAAACA GTCATTCGAG AGTTGCAGCC GCAACAGCAG
    CCTCGGACTA TTGAGCGGAG AGATCTGCAG TACATTACTA TCAGCAAGGA AGAATTGTCC
    TCTAGTGACA GCCTCTCTCC AGATCCCTGG AAACGAGATG CCAAGGAGAA ACTGGAGAAG
    CAGCAGCAGA TGCACATTGT GGACATGCTG AGCAAAGAGA TTCAGGATCT TCAGAACAAG
    CCTGATCGTA CAGCTGAAGA GAACGACCGC CTGCGCAAAC TCATGCTAGA GTGGCAGTTC
    CAAAAGCGAC TTCAGGAATC AAAACAAAAG GATGAAGATG ATGATGAAGA AGAGGACGAT
    GATGTGGATA CTATGCTTAT CATGCAGCGG CTGGAGGCAG AAAGAAGAGC AAGGTTACAG
    GATGAAGAAC GCAGACGACA GCAACAGTTA GAAGAAATGC GCAAGCGAGA AGCAGAAGAC
    AGGGCCAGGC AGGAAGAGGA GCGCCGACAG CAAGAGGAGG AGCGAACCAG GAGAGAGGCA
    GAAGAAAAGA GGCGACAGGA AGAGGAGTAT TACAATCGCT TAGAGGCTGA AAGGCGCAGG
    CAGCACGATG AAGCAGAACG AAGATTGCTG GAACCAGACG AGCCTGGAAT AAATTCATAC
    ACTGGATCTT CGGGAGCAAC GGTTGGGGCC CATGAAGTCT ATCGGGATCC AAGAGACAAA
    CGATCTAAAA GTCAAGATAC AGATTTACCT GGAGCACCAG AAAATCTGAC GTTCAGGGAA
    CGCCAGCGAC TTTTTTCACA GGGTCAAGAT GTATCCAATA AAGTAAAAGC CTCTAGGAAA
    CTAATGGAAC TGGAAAATGA GTTGAACACG AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139846.1
    CDS2885..8140
    Translation

    Target ORF information:

    RefSeq Version XM_015284360.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus afadin, adherens junction formation factor (AFDN), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284360.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGGCG GCGGCAGCCG GGAGGAGGAG CGGCGCAAAC TCGCCGACAT CATCAACCAC 
    TGGAACGCGA ACCGGCTGGA CCTGTTCGAG ATCAGCGCCC CCACCGAGGA TTTGGAATTT
    CATGGTGTAA TGAGATTTTA CTTTCAAGAC AAAGCGGCTG GAAACTTTGC AACAAAATGT
    ATCCGTGTCT CAAGTACTGC CACAACTCAG GATGTGATAG AGACTCTTGC AGAGAAGTTC
    CGGCCAGACA TGCGCATGTT GTCATCCCCT AAATACTCGC TCTATGAGGT GCATGTCAGT
    GGAGAAAGAA GATTAGATGT GGATGAGAAG CCTCTTGTTG TACAATTAAA CTGGAACAAA
    GATGATCGAG AAGGAAGGTT TGTTCTCAAG AATGAAAATG ATACGCTTCC TCCAAAGAAG
    GCTCAGAGTA ATGGCCCTGA AAAACAAGAA AAAGAAGGAG TAATACAGAA TTTCAAACGA
    ACTCTCTCAA AGAAAGAAAA GAAAGAGAAG AAGAGGCGTG AAAAAGAGGC ATTGCGACAA
    GCATCAGATA AAGATGATCG ACTTCTTCAT GGGGATGATA TTGAGAATTC TCGCCTTGCA
    GCTGAGGTTT ACAAAGATAT GCCTGAGACC AGCTTCACTC GCACAATATC TAATCCTGAG
    GTGGTTATGA AACGACGGCG GCAGCAAAAA CTAGAGAAGA GAATGCAGGA ATTTCGCAGT
    TCTGATGGCA GACCAGACTC AGGTGGGACG CTGAGAATTT ATGCGGACAG TTTAAAACCA
    AATATACCAT ACAAGACAAT CCTGTTGTCA ACTACAGATA CTGCAGACTT TGCAGTTATT
    GAAGCACTGG AGAAATATGG CTTGGAGAAG GAAAACCCCA AGGATTATTG TATTGCTCGT
    GTCATTCTGC CTCCTGGTGC TCAGCACTCT GATGATAAAG GTGCTAAAGA AACTATTCTA
    GATGATGATG AGTGTCCACT TCAGATATTC AGGGAATGGC CTAGTGATAA AGGAATACTA
    GTCTTTCAGT TGAAAAGGCG GCCTCCTGAT TATGTCCCAA AGAAATCCAA GAAAATAGCT
    GATGGAAAGC CATTAAAGGG GAAGGAAAGA GTTGACGGGT CTGGCTACGG CTCTTGCCTT
    CCTCCTGAGA AACTACCATA CCTGGTAGAA TTAAGCCCAG ATGGTTCTGA CTCCAGAGAT
    AAACCAAAGC TTTATCGTCT ACAATTAAGT GTCACTGAAG TTGGAACTGA AAAATTTGAT
    GACAATTCCA TTCAGTTATT TGGTCCAGGA ATTCAGCCTC ATCATTGTGA TCTTACTAAT
    ATGGATGGAG TTGTTACTGT AACCCCAAGA AACATTGATG CGGAAACGTA TGTAGAAGGT
    CAGCGTATTT CAGAAACCAC TATGCTGCAA AGTGGAATGA AGGTTCAGTT TGGGTCATCT
    CATGTGTTTA AGTTTGTGGA TCCCACTCAG GACCATATTA TTACTAAAAG ACCTATTGAT
    GCAAGCCTTA TGGTCAAAGG TCCAAGGCAT AAAGCTGGAG CTGTTCAGGA AACCACGTTT
    GATCTGGAGG GAGATGTTCA CAGTGGAACA GCGTTACCAG CGAGCAAGAG CACCAGCAGG
    CTTGATGGTG ACAGAACATC ATCAGCATCC AGCACAGCTG AACGAGGAAT GGTGAAGCCC
    ATGATTAGAA TAGAACAGCA GCAAGATTAT CGCAGACAGG AAAGCAGACC TCAGGATACC
    CATGGACCAG AACTGATCCT ACCTGCCAGT ATTGAATTCA GGGAAAGCTC TGAAGATGCC
    TTCTTATCTG CCATTATAAA TTATACAAAT AGCTCGACAG TTCATTTTAA ACTGTCACCC
    ACGTATGTTT TGTATATGAC ATGTCGGTAT GTATTGTCAA GCCAGTACAG ACCTGACATC
    ACTCCTACTG AGCGTACTCA CAAAGTTATT GCAATAGTCA ACAAGATGGT GAATATGATG
    GAAGGAGTCA TACAGAAACA GAAGAATATT GCTGGGGCAC TTGCCTTTTG GATGGCAAAT
    GCGTCTGAAT TGCTGAATTT TATAAAGCAA GACCGTGATC TGAGCCGAAT CACTGTGGAT
    GCTCAAGATG TTTTGGCGCA CTTGGTTCAA ATGGCATTCA AGTACCTAGT TCATTGTCTG
    CAGTCAGAGC TTAATAACTA CATGCCAGCA TTTCTTGATG ATCCAGAGGA AAATAATCCA
    CAGAGGCCTA AAATAGATGA TGTGCTGCAC ACATTGACTG GAGCTATGTC CTTGTTGCGA
    CGATGTAGAG TGAATGCTGC ACTGACTATA CAGCTCTTCT CCCAGTTGTT TCATTTTATC
    AACATGTGGC TTTTTAACAG ACTAGTTACG GCTCCAGATT CAGGGTTATG TTCCCACTAC
    TGGGGAGCTA TTGTTCGTCA GCAGTTGGGT CATATTGAGG CCTGGGCAGA AAAACAGGGT
    TTAGAATTGG CTGCTGATTG CCACTTAAGC AGAATAGTGC AGGCAACCAC ATTGCTCACC
    ATGGACAAGT ACTCACATGC TGATGTTCCA AATATTAACA GTACTTGCTT TAAGTTGAAT
    TCTCTACAGC TACAAGCTTT GTTGCAGAAT TATCACTGTG CTCCAGATGA ACCGCTTATA
    CCAACAGAAC TGATAGAGAA TGTAGTAACT GTTGCAGAAA ATACGGCTGA TGAACTTGCT
    CGCAGTGATG GCAGAGAAGT CCAATTGGAA GAGGATCCTG ACTTACAACT CCCTTTTCTT
    TTACCAGAAG ATGGCTATTC CTGTGATGTT GTCAGAAATG TTCCAAGTGG TTTACAAGAA
    TTTTTGGATC CCCTCTGTCA GAGAGGTTTT TGTAGGTTAA TACCTCATAC ACGGTCACCA
    GGCACATGGA CAATATACTT TGAAGGCGCA GACTATGAAA GCCACCTGTC ACATGAGAAT
    GCTGAGCTAG CTCAGCCTTT GAGGAAAGAA CCTGAGATTA TAACTGTAAC ACTAAAAAAA
    CAAAATGGAA TGGGCCTCAG CATTGTTGCT GCCAAGGGTG CTGGTCAAGA CAAACTTGGA
    ATATATGTCA AGTCTGTTGT GAAAGGAGGT GCTGCAGATG TGGATGGTCG TCTGGCAGCA
    GGGGATCAGC TGCTCAGTGT GGATGGTCGA AGCTTGGTGG GGCTGTCCCA GGAAAGGGCA
    GCAGAACTTA TGACGAGGAC AGGTTCTGTT GTAACACTGG AAGTAGCAAA ACAAGGAGCA
    ATATACCACG GTCTGGCAAC CCTGCTAAAT CAGCCATCTC CAATGATGCA AAGAGTTTCT
    GACCGCCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTTGAGCT GTATAACAAC
    TCCACGCAAA ATGGGTCACC TGAGAGCCCC CAGCTGCCAT GGACGGAGTA CAGTGAACCA
    AAGAAGCTGC CAGGAGATGA CAGATTAATG AAGAACAGAG CCGATCATCG CTCCAGCCCC
    AACGTAGCAA ATCAGCCTCC AAGCCCTGGG GGGAAGAATG CTTATGCATC TGGAACTACC
    ACCAAAATAA CCTCAGTCTC GACTGGAAAT CTTTGTACAG AGGAGCAAAC TCTGCCTCCA
    CGACCTGAAG CTTATCCTAT CCCAACACAG ACCTATACCA GAGAATATTT CACATTCCCA
    GCTTCAAAAT CCCAGGATCG AATGGGTCCA GCTCAGAGTC AGTGGCCAAA TTATGAAGAG
    AAGCCACAGA TCCAGGCAGA AAGTAATCAC TCAATCAATA CTACAATGCA GCGCGTGGCT
    CGATCCCAGG AAGAACTCCG AGATAAAGTT TTCCAGCCAG AGAGGAATCG TTTGGAAGTT
    GTTATCCGGA AGGACATAGA ATACATTGAT CGGAAGTCAG ACAGCGATCA GTGGATGAAT
    TCATCTGTGG ATTCTAGCAC ATCTAGCCAA GAGCACCTGA ACCATACATC CAAACCTGTT
    TCACCTGGTT CTTGCTTAAC TAAAAGTGGA CCTGGCCGTT GGAAAACTCC AGCTACGAGC
    CAGCCAACTC CAGTGGCCGT TTCCCAGCCC ATCAGAACAG ACCTTCCTCC TCCGCCGCCA
    CCTCCTCCGG TGCATTATGC AGCTGAATTT GATGAACCAC AAATGGATTT GCCTCTGCCT
    CCCCCTCCTC CACCGAACCA AGTAGGAGCA CAAGCATCTT CTCAGGTGGC TGCTGCTGAG
    CGTAAAAAAC GAGAAGAGCA TCAGAGGTGG TATGAAAAGG AAAAGGCACG TTTGGAAGAA
    GAACGAGAAA GGAAACGCCG TGAGCAAGAG AGAAAACTGG GCCAAATGCG TTCGCAGCCA
    CTAATTCCTC CCCAGCCTGT GGTTCCTCCT GTTTCCCTTC AGCAAGTGAA ATCGGAAAAA
    ACTTCAACTT TCCAGAGGTC TCAAGAAACA GTCATTCGAG AGTTGCAGCC GCAACAGCAG
    CCTCGGACTA TTGAGCGGAG AGATCTGCAG TACATTACTA TCAGCAAGGA AGAATTGTCC
    TCTAGTGACA GCCTCTCTCC AGATCCCTGG AAACGAGATG CCAAGGAGAA ACTGGAGAAG
    CAGCAGCAGA TGCACATTGT GGACATGCTG AGCAAAGAGA TTCAGGATCT TCAGAACAAG
    CCTGATCGTA CAGCTGAAGA GAACGACCGC CTGCGCAAAC TCATGCTAGA GTGGCAGTTC
    CAAAAGCGAC TTCAGGAATC AAAACAAAAG GATGAAGATG ATGATGAAGA AGAGGACGAT
    GATGTGGATA CTATGCTTAT CATGCAGCGG CTGGAGGCAG AAAGAAGAGC AAGGTTACAG
    GATGAAGAAC GCAGACGACA GCAACAGTTA GAAGAAATGC GCAAGCGAGA AGCAGAAGAC
    AGGGCCAGGC AGGAAGAGGA GCGCCGACAG CAAGAGGAGG AGCGAACCAG GAGAGAGGCA
    GAAGAAAAGA GGCGACAGGA AGAGGAGTAT TACAATCGCT TAGAGGCTGA AAGGCGCAGG
    CAGCACGATG AAGCAGAACG AAGATTGCTG GAACCAGACG AGCCTGGAAT AAATTCATAC
    ACTGGATCTT CGGGAGCAAC GGTTGGGGCC CATGAAGTCT ATCGGGATCC AAGAGACAAA
    CGATCTAAAA GTCAAGATAC AGATTTACCT GGAGCACCAG AAAATCTGAC GTTCAGGGAA
    CGCCAGCGAC TTTTTTCACA GGGTCAAGAT GTATCCAATA AAGTAAAAGC CTCTAGGAAA
    CTAATGGAAC TGGAAAATGA GTTGAACACG AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.