NF1 cDNA ORF clone, Gallus gallus(chicken)

The following NF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa145673 XM_025142091.1
Latest version!
Gallus gallus neurofibromin 1 (NF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa17415 XM_004946785.3
Latest version!
Gallus gallus neurofibromin 1 (NF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa17415 XM_025142089.1
Latest version!
Gallus gallus neurofibromin 1 (NF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa17416 XM_415914.6
Latest version!
Gallus gallus neurofibromin 1 (NF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa17417 XM_025142090.1
Latest version!
Gallus gallus neurofibromin 1 (NF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa17419 XM_003642416.3
Latest version!
Gallus gallus neurofibromin 1 (NF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa44369 XM_015295853.1
Latest version!
Gallus gallus neurofibromin 1 (NF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa44369 XM_015295852.1
Latest version!
Gallus gallus neurofibromin 1 (NF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa44370 XM_015295854.1
Latest version!
Gallus gallus neurofibromin 1 (NF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa44371 XM_015295855.1
Latest version!
Gallus gallus neurofibromin 1 (NF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa44372 XM_015295856.1
Latest version!
Gallus gallus neurofibromin 1 (NF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OGa17415 XM_004946785.2 Gallus gallus neurofibromin 1 (NF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa17416 XM_415914.5 Gallus gallus neurofibromin 1 (NF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa145673
    Clone ID Related Accession (Same CDS sequence) XM_025142091.1
    Accession Version XM_025142091.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofibromin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGTGGCCTG GGGTAAGAAG GGGCACACAG CAACCGAAGG ATACGATGCG ACTAGACGAG 
    ACTATGCTGG TAAAACAGTT GCTGCCAGAA ATCTGCCACT TCATCCACAC TTACCGTGAA
    GGGAACCAGC ATGCAGCTGA ACTACGCAAT TCTGCCTCTG GGGTTCTTTT TTCTCTTAGC
    TGCAATAACT TTAACGCTGT GTTCAGCCGC ATTTCCACCA GATTGCAGGA ACTGACTGTT
    TGCTCAGAAG ATAATGCCGA TGTTCACGAC ATTGAACTTC TACAGTACAT CAGTGTGGAT
    TGTTCAAAGC TAAAAAGACT CTTGCAAGAG ACTGTATTCA AGTTTAAAGC CCTTAAGAAA
    GTAGCTCAGT TAGCTGTTAT CAACAGTCTT GAAAAGGCAT TTTGGAACTG GGTGGAAAAC
    TATCCTGATG AATTCACAAA GCTGTATCAA ACACCACAGA CTGATATGGC CGATTGTGCA
    GAGAAGTTGT TTGACTTAGT GGATGGCTTT GCAGAGAGCA CAAAACGTAA AGCAGCGGTG
    TGGCCACTAC AAATCATACT CCTGGTCCTG TGTCCAGAGA TAATTCAAGA TATAGCAAAG
    GATGTAGTAG AGGAAACTAA AATGAACAAG AAACTGTTCC TGGACAATCT CAGGAAAGCA
    CTTGCAGGCC ACAGTGGGAG CAGGCAGCTG ACTGAAAGCG CTGCTATTGC TTGTGTTAAA
    CTGTGCAAAG CATCTACCTA CATCAACTGG GAGGATAATT CTGTCATTTT TCTACTAGTG
    CAGTCCATGG TAGTAGATCT TAAGAATTTG CTGTTTAATC CGAGCAAACC TTTTTCAAGA
    AGCAATCAGA ATGCAGATGT AGACCTTATG ATCGACTGCT TAGTTTCATG CTTCCGCATA
    AACCCGCATA ATAACCAACA CTTTAAGATC TGCTTGGCAC AGAGTTCCCC ATCAACATTT
    CATTATGTAC TAGTAAATTC ACTCCACCGA ATAATCACAA ATTCTGCATT GGACTGGTGG
    CCCAGGATTG ATGCTGTGTA CTGCCATTCC GTGGAGCTTC GCAGCATGTT TAGTGAGACA
    CTTCACAAAG CTATTCAGGG CTGTGGGGCA CATCCAGCAA TTCGCATGAC TCCGATGCCA
    ACCAGTCACT GTTTTGCATG GCAGTGGGCC TCTGTTGCTA GTCTGACATT TAAAGAGAAA
    ATGACAAGCC TTAAATTTAA AGAAAAGCCT ACTGACTTGG AAACCAAAAG CTACAAGTTC
    CTGCTGTTAT CCTTGGTAAA ACTGATCCAT GCTGATCCAA AGCTTTTGCT GTGTAATCCA
    AGGAAGCAAG GGCCTGAGAC ACAAGGCAGC ACTGCTGAAT TAATTACAGG CCTTGTACAA
    CTCGTTCCAC AATCCAGCAT GCCAGATATA GCACAAGAAG CCATGGAGGC CTTGCTAGTT
    CTTCACCAGT TAGACAGCAT TGACTTATGG AATCCTGATG CACCTATAGA AACTTTCTGG
    GAAATTAGTT CACAAATGCT TTTTTATATC TGCAAGAAAT TAACCAGTCA TCAGATGCTC
    AGCAGTACAG AAATCCTCAA GTGGCTGCGA GAGATTCTCA TCTGTAGGAA TAAATTTCTT
    CTAAAAAATA AACAATCAGA CAGAACTTCC TGTCACTTTC TCTTCCTTTA TGATGTCTCT
    GGAGGTGGAG CTAGTCAGAT GTCCCTTGAC CACGAAGAGA TGATGCGCTG TGCTCCCGGA
    GCCACCCTCA GAAAAGGAAA AGGGAATTCT TCCATGGAAA GTACAGCTGG ATGCAGTGGA
    ACACCCATTT GTCGCCAGGC CCAAACAAAA TTGGAAGTTG CCTTATACAT GTTTTTGTGG
    AGTCCGGATA TTGAGGCAGT CCTTGTTGCA ATGTCCTGTT TCCGTCACCT CTGTGAGGAA
    GCAGATATCA GATGTGGAGT AGATGAAGTT TCAGTTCATA ATTTTTTGCC AAACTACAAC
    ACTTTCATGG AGTTTGCATC TGTTAGCAAT ATGATGTCAA CAGGGAGAGC AGCTCTTCAG
    AAAAGAGTGA TGGCTTTATT AAGACGTATT GAACATCCAA CTGCAGGGAA CACAGAGGCT
    TGGGAGGATA CACATGCAAA ATGGGAACAA GCTACAAAAC TAATCCTTAA CTATCCAAAG
    ACCAAAATGG AAGACGGGCA GGTTACTGAA AGTCTTCATA AGACAATTGT TAAGAGACGA
    ATGTCACATG TTAGTGGGGG AGGCTCCATA GACTTGTCTG ACACAGATTC TCTTCAGGAA
    TGGATCAACA TGACGGGATT CCTCTGTGCC CTCGGAGGAG TGTGCCTGCA GCATCGTAGC
    AATGCAGGAT TAGCAACCTA CAGTCCACCC ATGGGCCCAG TCAATGAGCG TAAGGGCTCC
    ATGATATCTA TGGTATCCAC TGAGGGAAAT GCAGAGACTC CTGTTAGCAA ATTCATAGAT
    CGATTACTGA CTCTGATGGT CTGTAACCAT GAGAAAGTGG GACTACAAAT ACGGACTAAT
    ATTAAAGATC TGGTGGGTTT GGAGTTGAGT CCAGCACTTT ATCCAATGCT ATTTAACAAA
    TTGAAGAATA CCATCAGCAA GTTTTTTGAT TCTCAAGGAC AGGTTTTGTT AACTGAGACC
    AACACACAGT TTGTAGAACA AACCATTGCT ATAATGAAGA ACTTGCTTGA CAATCATACA
    GAAGGGAGCT CAGAACATCT TGGACAAGCT AGTATTGAGA CAATGATGCT AAACCTGGTT
    AGGTATGTGC GTGTACTTGG AAATTTGGTA CATGCAATTC AAATAAAAAC TAAACTCTGT
    CAGTTGGTGG AAGTAATGAT GGAGCGGAGA GATGACCTTT CATTTTGCCA AGAAATGAAA
    TTTAGGAACA AAATGGTGGA ATATCTGACA GACTGGGTTA TGGGAACATC TAATCAAGCA
    ACAGATGAAG ATGTGAAGTG CTTGACAAGA GATTTGGACC AAGCAAGTAT GGAAGCAGTG
    GTCTCTCTTC TTGCTGGGCT TCCGTTACAG CCTGAAGAAG GAGATGGTGT TGAGTTGATG
    GAAGCCAAAT CTCAGTTATT TCTTAAATAT TTCACACTGT TTATGAACCT TCTAAATGAC
    TGTAGTGAAG TTGAAGATGA AAGTGCACAA ACAGGTGGCA GGAAACGTGG GATGTCCCGA
    AGACTGGCTT CGCTGCGGCA CTGCACTGTT CTTGCTATGT CAAATTTACT AAATGCAAAT
    GTGGACAGTG GTCTTATGCA TTCAATAGGC TTGGGCTATC ATAAAGACCT CCAGACAAGA
    GCTACATTTA TGGAAGTCCT CACCAAAATT CTACAGCAGG GAACAGAATT TGATACGTTA
    GCAGAAACGG TGCTGGCAGA TCGGTTTGAG AGGCTGGTGG AGCTAGTTAC AATGATGGGT
    GATCAGGGGG AGCTTCCTAT TGCTATGGCT TTAGCAAATG TGGTGCCTTG TTCTCAGTGG
    GATGAGCTAG CTCGTGTTCT GGTCACACTC TTTGATTCCC GGCACTTGCT CTACCAGCTA
    CTTTGGAATA TGTTTTCAAA GGAAGTTGAA CTGGCAGATT CCATGCAGAC ACTCTTCCGA
    GGAAACAGCT TAGCTAGTAA AATAATGACT TTCTGTTTTA AGGTATATGG TGCTACTTAT
    TTGCAGAAGC TTTTGGAACC TTTATTAAGA ACAGTGATCA CATCCTCTGA ATGGCAGCAT
    GTTAGCTTTG AGGTGGATCC TACAAGACTG GAGCCTACAG AAAGCCTTGA AGAGAACCAG
    CGGAGTCTCT TGCAAATGAC AGAAAAATTT TTTCATGCAA TCATTAATTC TTCTTCGGAA
    TTCCCACCAC AACTTCGCAG TGTGTGCCAT TGTTTGTACC AGGCAACTTG CCACTCTCTA
    CTGAATAAAG CTACCGTAAA AGAAAAAAAG GAAAACAAAA AATCAGTTGT TAGTCAGCGA
    TTTCCTCAGA ACAGCATTGG AGCAGTTGGG AGTGCCATGT TCCTGCGGTT CATCAACCCT
    GCGATCGTCT CCCCGTACGA GGCAGGGATT TTAGATAAAA AGCCTCCGCC CAGAATTGAG
    AGGGGACTGA AACTGATGTC AAAGATACTT CAGAGTATTG CCAACCACGT CCTGTTTACA
    AAAGAAGAAC ATATGAGGCC TTTTAATGAC TTCGTAAAAA GCAATTTTGA TGCAGCTCGA
    AGGTTTTTCC TTGATATTGC ATCTGATTGC CCTGCGAGTG ACACTGTCAA TCACAGCCTG
    TCTTTCATCA GTGATGGCAA TGTTCTTGCT TTGCACCGGT TGTTGTGGAA CAATCAGGAG
    AAAATTGGCC AGTACCTTTC CAGTAATAGG GACCACAAAG CTGTTGGAAG GCGACCTTTT
    GACAAAATGG CTACTCTTCT TGCCTATCTG GGTCCACCAG AGCACAAACC TGTGGCAGAT
    ACACATTGGT CCAGTTTAAA CCTCACCAGT TCGAAATTTG AAGAATTTAT GACAAGGCAT
    CAGGTACATG AAAAAGAAGA GTTCAAAGCA TTGAAAACGT TAAATATTTT CTACCAGGCT
    GGGACATCCA AAGCTGGCAA TCCTGTTTTC TACTACATTG CTCGGCGTTT CAAGACTGGT
    CAGATCAATG GAGATTTATT GATATACCAC GTCCTGCTAA CTCTGAAGCC ATACTACGCA
    AAGCCATATG AAATTGTAGT GGACCTCACA CATGCTGGCC CCAGTAATCG CTTTAAAACA
    GACTTTCTTT CTAAATGGTT TGTAGTTTTT CCAGGCTTTG CTTATGAAAA TGTCTCAGCA
    GTTTTTATTT ATAACTGTAA TTCTTGGGTC AGAGAATACA CCAAATACCA CGAAAGACTC
    TTGACAGGTT TGAAAGGAAG CAAAAGGCTT ATTTTCATAG ACTCTCCAGG AAAACTGGCG
    GAGCACATTG AACATGACCA GCAGAAACTT CCAGCAGCTA CCTTAGCTTT GGAAGAGGAC
    TTGAAAGTAT TTCACAATGC CCTCAAATTG GCTCACAAAG ACACCAAAGT GTCTATTAAA
    GTTGGATCAA CAGCTGTGCA GGTGACATCA GCAGAACGAA CAAGAGTCCT GGGACAGACA
    GTGTTTTTAA ATGATATATA CTACGCCTCT GAAATTGAGG AAATATGTCT TGTTGATGAG
    AATCAGTTTA CATTGACGAT TGCCAACCAG GGAACGCCAC TCACCTTCAT GCATCAGGAG
    TGTGAAGCCA TTGTGAGGTC CATTATTCAT ATACGGACAC GGTGGGAGCT GTCGCAACCA
    GACTCCATCC CACAGCACAC TAAAATTCGC CCTAAGGATG TTCCCGGAAC ACTACTGAAC
    ATAGCATTGC TCAACCTGGG AAGCTCAGAC CCGAGTTTGC GGTCTGCTGC CTATAATCTT
    CTGTGTGCCT TAACCTGTAC CTTTAATTTA AAGATTGAGG GCCAGTTACT GGAAACTTCA
    GGTCTGTGTA TTCCTGCCAA CAACACGCTC TTTATTGTGT CTATTAGTAA GACTCTTGCA
    GCTAATGAGC CACACCTTAC CCTAGAGTTC TTGGAAGAGT GTATTTCAGG ATTTAGCAAA
    TCTAGTATTG AACTGAAACA TCTTTGTCTG GAATATATGA CTCCCTGGCT GTCCAATCTG
    GTCCGATTCT GCAAACACAA CGACGATGCG AAGCGCCAGC GAGTCACTGC CATTCTTGAT
    AAGCTTATAA CAATGACAAT AAATGAAAAA CAGATGTACC CTTCTATTCA GGCTAAAATA
    TGGGGAAGTC TTGGACAGAT TACAGATCTC CTTGACGTAG TGCTGGACAG TTTCATCAAA
    ACTAGTGCCA CGGGGGGCTT AGGATCCATT AAAGCTGAGG TTATGGCAGA CACAGCAGTA
    GCGCTTGCTT CTGGAAATGT AAAATTGGTT TCAAGCAAAG TGATTGGAAG GATGTGCAAA
    ATAATTGACA AGACTTGTCT GTCTCCAACT CCTACACTGG AACAGCACCT GATGTGGGAT
    GATATTGCCA TTCTAGCACG TTACATGCTG ATGCTCTCCT TCAACAATTC TCTGGATGTG
    GCTGCACATC TCCCCTACCT CTTTCATGTG GTTACTTTGT TGGTGGCCAC TGGACCCCTT
    TCTCTCAGAG CTTCCACTCA TGGTCTTGTC ATCAACATCA TTCATTCTCT GTGCACTTGC
    TCACAGCTTC ATTTTAGTGA GGAGACCAAG CAAGTTTTAA GACTGAGCTT GACTGAATTT
    TCTCTGCCTA AATTTTATTT GCTGTTTGGA ATCAGCAAAG TGAAATCAGC TGCAGTCATA
    GCATTCCGCT CCAGCTATCG AGACAGGTCA TTTTCACCTG GCTCCTACGA GAGGGAGACT
    TTTGCACTGA CCTCCTTGGA AACTGTTACT GAAGCTTTGC TGGAGATCAT GGAGGCTTGT
    ATGAGAGATA TTCCAACATG CAAGTGGCTG GACCAGTGGA CCGAACTAGC ACAAAAGTTT
    GCATTCCAGT ACAATCCATC CCTGCAGCCA AGAGCACTCG TTGTCTTTGG CTGTATAAGT
    AAGCGTGTCT CTCATGGACA AATAAAACAA ATAATCCGAA TTCTTAGCAA GGGGCTTGAG
    AGCTGTTTAA AAGGCCCTGA TAATTACAAT AGCCAAGTTC TAATAGAAGC TACGGTTATA
    GCACTCACCA AACTGCAGCC TCTTCTTAAC AAGGACTCCC CCATGCACAA GGCCCTCTTC
    TGGGTAGCCA TGGCAGTACT GCAGTTGGAT GAAGTCAACC TATACTCAGC AGGTACAGCA
    CTGCTTGAAC AGAATCTGCA TACCTTGGAT AGCCTTCGAG TGTTCAATGA TAAGAGCCCA
    GAAGAGGTGT TCATGGAGAT CAGGAGACCA CTTGAATGGC ACTGCAAACA GATGGATCAT
    TTTGTTGGGC TAAATTTCAA CTCCAACTTC AACTTTGCAC TAGTGGGGCA CCTCCTGAAA
    GGGTACAGGC ATCCTTCACC TACCACTGTA GCAAGAACAG TAAGGATTCT ACATACACTA
    CTAGCTCTGG TTAACAAACA CAGGAACTGT GACAAGTTTG AGGTGAACAC CCAGAGCGTG
    GCTTACCTAG CAGCTTTGCT GACAGTGTCT GAGGAAGTTC GAAGTCGCTG CAGCCTAAAA
    CATAGGAAGT CTCTCTTGCT GACAGATGTT GCAATGGAAA ATGTTCCTAT GGATACATAT
    CCCATACATC ACAGTGACAC TACCTATAGG ACACTAAAGG AAAATCAACC ATGGTCATCA
    CCAAAGGGAT CAGACAGACA CCTGGCTGCC AGTTACCCGA CAGTGGGACA GATAAGCCCT
    CGAACACGAA AGTCCATGAG TTTGGATATG GGGCAGCCTT CACAAGCGAA CACTAAAAAG
    CTTCTAGGTA CAAGGAAAAG TTTTGACCAT TTGATATCGG ACACAAAGGC TCCTAAAAGG
    CAAGACATGG AATCTGGAAT CACAACGCCT CCCAAAATGA GGAGAGTAGC CGAAAATGAT
    TATGAAATGG AAACCCAGAG AATATCACCG CAGCAGCACC CCCATCTCCG GAAAGTTTCT
    GTTTCTGAGT CAAATGTCCT CCTGGATGAA GAAGTTCTGA CTGATCCAAA AATTCAAGCA
    CTGCTGCTTA CAGTTCTTGC CACGTTAGTG AAGTACACTA CTGATGAATT TGACCAGCGG
    ATTCTGTATG AGTATTTAGC AGAAGCCAGT GTTGTCTTCC CAAAGGTCTT TCCTGTTGTG
    CATAATTTAT TGGATTCCAA GATCAATACC CTCTTGTCGC TGTGTCAAGA TCCAAATTTG
    TTGAATCCAA TTCATGGGAT TGTTCAGAGT GTTGTCTACC ATGAGGAGTC TCCACCCCAA
    TACCAAACTT CTTATCTACA AAGTTTTGGA TTTAATGGGT TGTGGAGGTT TGCTGGCCCA
    TTTTCCAAGC AAACACAAAT CCCAGACTAT GCTGAGCTCA TAGTGAAGTT TCTTGATGCC
    TTGATTGACA CGTATTTGCC TGGAATTGAT GAGGAAACCA GTGAAGAATC TCTCCTGACA
    CCCACGTCTC CATATCCTCC CGCAGTGCAG AGCCAGCTTA GCATTACAGC TAACCTTAAC
    CTTTCCAATT CCATGACCTC GCTCGCAACT TCCCAGCACT CCCCAGGGAT CGACAAGGAG
    AACGTGGAGC TCTCGCCCAC CACTGGCCAC ACCAACAGCG GGAGGATGCG CCACGGCTCC
    GCCAGCCAGG TGCAAAAGCA GCGAAGTGCT GGCAGCTTGA AACGTCACAG CATTAAGAAG
    ATTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024997859.1
    CDS100..8388
    Translation

    Target ORF information:

    RefSeq Version XM_025142091.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofibromin 1 (NF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025142091.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGTGGCCTG GGGTAAGAAG GGGCACACAG CAACCGAAGG ATACGATGCG ACTAGACGAG 
    ACTATGCTGG TAAAACAGTT GCTGCCAGAA ATCTGCCACT TCATCCACAC TTACCGTGAA
    GGGAACCAGC ATGCAGCTGA ACTACGCAAT TCTGCCTCTG GGGTTCTTTT TTCTCTTAGC
    TGCAATAACT TTAACGCTGT GTTCAGCCGC ATTTCCACCA GATTGCAGGA ACTGACTGTT
    TGCTCAGAAG ATAATGCCGA TGTTCACGAC ATTGAACTTC TACAGTACAT CAGTGTGGAT
    TGTTCAAAGC TAAAAAGACT CTTGCAAGAG ACTGTATTCA AGTTTAAAGC CCTTAAGAAA
    GTAGCTCAGT TAGCTGTTAT CAACAGTCTT GAAAAGGCAT TTTGGAACTG GGTGGAAAAC
    TATCCTGATG AATTCACAAA GCTGTATCAA ACACCACAGA CTGATATGGC CGATTGTGCA
    GAGAAGTTGT TTGACTTAGT GGATGGCTTT GCAGAGAGCA CAAAACGTAA AGCAGCGGTG
    TGGCCACTAC AAATCATACT CCTGGTCCTG TGTCCAGAGA TAATTCAAGA TATAGCAAAG
    GATGTAGTAG AGGAAACTAA AATGAACAAG AAACTGTTCC TGGACAATCT CAGGAAAGCA
    CTTGCAGGCC ACAGTGGGAG CAGGCAGCTG ACTGAAAGCG CTGCTATTGC TTGTGTTAAA
    CTGTGCAAAG CATCTACCTA CATCAACTGG GAGGATAATT CTGTCATTTT TCTACTAGTG
    CAGTCCATGG TAGTAGATCT TAAGAATTTG CTGTTTAATC CGAGCAAACC TTTTTCAAGA
    AGCAATCAGA ATGCAGATGT AGACCTTATG ATCGACTGCT TAGTTTCATG CTTCCGCATA
    AACCCGCATA ATAACCAACA CTTTAAGATC TGCTTGGCAC AGAGTTCCCC ATCAACATTT
    CATTATGTAC TAGTAAATTC ACTCCACCGA ATAATCACAA ATTCTGCATT GGACTGGTGG
    CCCAGGATTG ATGCTGTGTA CTGCCATTCC GTGGAGCTTC GCAGCATGTT TAGTGAGACA
    CTTCACAAAG CTATTCAGGG CTGTGGGGCA CATCCAGCAA TTCGCATGAC TCCGATGCCA
    ACCAGTCACT GTTTTGCATG GCAGTGGGCC TCTGTTGCTA GTCTGACATT TAAAGAGAAA
    ATGACAAGCC TTAAATTTAA AGAAAAGCCT ACTGACTTGG AAACCAAAAG CTACAAGTTC
    CTGCTGTTAT CCTTGGTAAA ACTGATCCAT GCTGATCCAA AGCTTTTGCT GTGTAATCCA
    AGGAAGCAAG GGCCTGAGAC ACAAGGCAGC ACTGCTGAAT TAATTACAGG CCTTGTACAA
    CTCGTTCCAC AATCCAGCAT GCCAGATATA GCACAAGAAG CCATGGAGGC CTTGCTAGTT
    CTTCACCAGT TAGACAGCAT TGACTTATGG AATCCTGATG CACCTATAGA AACTTTCTGG
    GAAATTAGTT CACAAATGCT TTTTTATATC TGCAAGAAAT TAACCAGTCA TCAGATGCTC
    AGCAGTACAG AAATCCTCAA GTGGCTGCGA GAGATTCTCA TCTGTAGGAA TAAATTTCTT
    CTAAAAAATA AACAATCAGA CAGAACTTCC TGTCACTTTC TCTTCCTTTA TGATGTCTCT
    GGAGGTGGAG CTAGTCAGAT GTCCCTTGAC CACGAAGAGA TGATGCGCTG TGCTCCCGGA
    GCCACCCTCA GAAAAGGAAA AGGGAATTCT TCCATGGAAA GTACAGCTGG ATGCAGTGGA
    ACACCCATTT GTCGCCAGGC CCAAACAAAA TTGGAAGTTG CCTTATACAT GTTTTTGTGG
    AGTCCGGATA TTGAGGCAGT CCTTGTTGCA ATGTCCTGTT TCCGTCACCT CTGTGAGGAA
    GCAGATATCA GATGTGGAGT AGATGAAGTT TCAGTTCATA ATTTTTTGCC AAACTACAAC
    ACTTTCATGG AGTTTGCATC TGTTAGCAAT ATGATGTCAA CAGGGAGAGC AGCTCTTCAG
    AAAAGAGTGA TGGCTTTATT AAGACGTATT GAACATCCAA CTGCAGGGAA CACAGAGGCT
    TGGGAGGATA CACATGCAAA ATGGGAACAA GCTACAAAAC TAATCCTTAA CTATCCAAAG
    ACCAAAATGG AAGACGGGCA GGTTACTGAA AGTCTTCATA AGACAATTGT TAAGAGACGA
    ATGTCACATG TTAGTGGGGG AGGCTCCATA GACTTGTCTG ACACAGATTC TCTTCAGGAA
    TGGATCAACA TGACGGGATT CCTCTGTGCC CTCGGAGGAG TGTGCCTGCA GCATCGTAGC
    AATGCAGGAT TAGCAACCTA CAGTCCACCC ATGGGCCCAG TCAATGAGCG TAAGGGCTCC
    ATGATATCTA TGGTATCCAC TGAGGGAAAT GCAGAGACTC CTGTTAGCAA ATTCATAGAT
    CGATTACTGA CTCTGATGGT CTGTAACCAT GAGAAAGTGG GACTACAAAT ACGGACTAAT
    ATTAAAGATC TGGTGGGTTT GGAGTTGAGT CCAGCACTTT ATCCAATGCT ATTTAACAAA
    TTGAAGAATA CCATCAGCAA GTTTTTTGAT TCTCAAGGAC AGGTTTTGTT AACTGAGACC
    AACACACAGT TTGTAGAACA AACCATTGCT ATAATGAAGA ACTTGCTTGA CAATCATACA
    GAAGGGAGCT CAGAACATCT TGGACAAGCT AGTATTGAGA CAATGATGCT AAACCTGGTT
    AGGTATGTGC GTGTACTTGG AAATTTGGTA CATGCAATTC AAATAAAAAC TAAACTCTGT
    CAGTTGGTGG AAGTAATGAT GGAGCGGAGA GATGACCTTT CATTTTGCCA AGAAATGAAA
    TTTAGGAACA AAATGGTGGA ATATCTGACA GACTGGGTTA TGGGAACATC TAATCAAGCA
    ACAGATGAAG ATGTGAAGTG CTTGACAAGA GATTTGGACC AAGCAAGTAT GGAAGCAGTG
    GTCTCTCTTC TTGCTGGGCT TCCGTTACAG CCTGAAGAAG GAGATGGTGT TGAGTTGATG
    GAAGCCAAAT CTCAGTTATT TCTTAAATAT TTCACACTGT TTATGAACCT TCTAAATGAC
    TGTAGTGAAG TTGAAGATGA AAGTGCACAA ACAGGTGGCA GGAAACGTGG GATGTCCCGA
    AGACTGGCTT CGCTGCGGCA CTGCACTGTT CTTGCTATGT CAAATTTACT AAATGCAAAT
    GTGGACAGTG GTCTTATGCA TTCAATAGGC TTGGGCTATC ATAAAGACCT CCAGACAAGA
    GCTACATTTA TGGAAGTCCT CACCAAAATT CTACAGCAGG GAACAGAATT TGATACGTTA
    GCAGAAACGG TGCTGGCAGA TCGGTTTGAG AGGCTGGTGG AGCTAGTTAC AATGATGGGT
    GATCAGGGGG AGCTTCCTAT TGCTATGGCT TTAGCAAATG TGGTGCCTTG TTCTCAGTGG
    GATGAGCTAG CTCGTGTTCT GGTCACACTC TTTGATTCCC GGCACTTGCT CTACCAGCTA
    CTTTGGAATA TGTTTTCAAA GGAAGTTGAA CTGGCAGATT CCATGCAGAC ACTCTTCCGA
    GGAAACAGCT TAGCTAGTAA AATAATGACT TTCTGTTTTA AGGTATATGG TGCTACTTAT
    TTGCAGAAGC TTTTGGAACC TTTATTAAGA ACAGTGATCA CATCCTCTGA ATGGCAGCAT
    GTTAGCTTTG AGGTGGATCC TACAAGACTG GAGCCTACAG AAAGCCTTGA AGAGAACCAG
    CGGAGTCTCT TGCAAATGAC AGAAAAATTT TTTCATGCAA TCATTAATTC TTCTTCGGAA
    TTCCCACCAC AACTTCGCAG TGTGTGCCAT TGTTTGTACC AGGCAACTTG CCACTCTCTA
    CTGAATAAAG CTACCGTAAA AGAAAAAAAG GAAAACAAAA AATCAGTTGT TAGTCAGCGA
    TTTCCTCAGA ACAGCATTGG AGCAGTTGGG AGTGCCATGT TCCTGCGGTT CATCAACCCT
    GCGATCGTCT CCCCGTACGA GGCAGGGATT TTAGATAAAA AGCCTCCGCC CAGAATTGAG
    AGGGGACTGA AACTGATGTC AAAGATACTT CAGAGTATTG CCAACCACGT CCTGTTTACA
    AAAGAAGAAC ATATGAGGCC TTTTAATGAC TTCGTAAAAA GCAATTTTGA TGCAGCTCGA
    AGGTTTTTCC TTGATATTGC ATCTGATTGC CCTGCGAGTG ACACTGTCAA TCACAGCCTG
    TCTTTCATCA GTGATGGCAA TGTTCTTGCT TTGCACCGGT TGTTGTGGAA CAATCAGGAG
    AAAATTGGCC AGTACCTTTC CAGTAATAGG GACCACAAAG CTGTTGGAAG GCGACCTTTT
    GACAAAATGG CTACTCTTCT TGCCTATCTG GGTCCACCAG AGCACAAACC TGTGGCAGAT
    ACACATTGGT CCAGTTTAAA CCTCACCAGT TCGAAATTTG AAGAATTTAT GACAAGGCAT
    CAGGTACATG AAAAAGAAGA GTTCAAAGCA TTGAAAACGT TAAATATTTT CTACCAGGCT
    GGGACATCCA AAGCTGGCAA TCCTGTTTTC TACTACATTG CTCGGCGTTT CAAGACTGGT
    CAGATCAATG GAGATTTATT GATATACCAC GTCCTGCTAA CTCTGAAGCC ATACTACGCA
    AAGCCATATG AAATTGTAGT GGACCTCACA CATGCTGGCC CCAGTAATCG CTTTAAAACA
    GACTTTCTTT CTAAATGGTT TGTAGTTTTT CCAGGCTTTG CTTATGAAAA TGTCTCAGCA
    GTTTTTATTT ATAACTGTAA TTCTTGGGTC AGAGAATACA CCAAATACCA CGAAAGACTC
    TTGACAGGTT TGAAAGGAAG CAAAAGGCTT ATTTTCATAG ACTCTCCAGG AAAACTGGCG
    GAGCACATTG AACATGACCA GCAGAAACTT CCAGCAGCTA CCTTAGCTTT GGAAGAGGAC
    TTGAAAGTAT TTCACAATGC CCTCAAATTG GCTCACAAAG ACACCAAAGT GTCTATTAAA
    GTTGGATCAA CAGCTGTGCA GGTGACATCA GCAGAACGAA CAAGAGTCCT GGGACAGACA
    GTGTTTTTAA ATGATATATA CTACGCCTCT GAAATTGAGG AAATATGTCT TGTTGATGAG
    AATCAGTTTA CATTGACGAT TGCCAACCAG GGAACGCCAC TCACCTTCAT GCATCAGGAG
    TGTGAAGCCA TTGTGAGGTC CATTATTCAT ATACGGACAC GGTGGGAGCT GTCGCAACCA
    GACTCCATCC CACAGCACAC TAAAATTCGC CCTAAGGATG TTCCCGGAAC ACTACTGAAC
    ATAGCATTGC TCAACCTGGG AAGCTCAGAC CCGAGTTTGC GGTCTGCTGC CTATAATCTT
    CTGTGTGCCT TAACCTGTAC CTTTAATTTA AAGATTGAGG GCCAGTTACT GGAAACTTCA
    GGTCTGTGTA TTCCTGCCAA CAACACGCTC TTTATTGTGT CTATTAGTAA GACTCTTGCA
    GCTAATGAGC CACACCTTAC CCTAGAGTTC TTGGAAGAGT GTATTTCAGG ATTTAGCAAA
    TCTAGTATTG AACTGAAACA TCTTTGTCTG GAATATATGA CTCCCTGGCT GTCCAATCTG
    GTCCGATTCT GCAAACACAA CGACGATGCG AAGCGCCAGC GAGTCACTGC CATTCTTGAT
    AAGCTTATAA CAATGACAAT AAATGAAAAA CAGATGTACC CTTCTATTCA GGCTAAAATA
    TGGGGAAGTC TTGGACAGAT TACAGATCTC CTTGACGTAG TGCTGGACAG TTTCATCAAA
    ACTAGTGCCA CGGGGGGCTT AGGATCCATT AAAGCTGAGG TTATGGCAGA CACAGCAGTA
    GCGCTTGCTT CTGGAAATGT AAAATTGGTT TCAAGCAAAG TGATTGGAAG GATGTGCAAA
    ATAATTGACA AGACTTGTCT GTCTCCAACT CCTACACTGG AACAGCACCT GATGTGGGAT
    GATATTGCCA TTCTAGCACG TTACATGCTG ATGCTCTCCT TCAACAATTC TCTGGATGTG
    GCTGCACATC TCCCCTACCT CTTTCATGTG GTTACTTTGT TGGTGGCCAC TGGACCCCTT
    TCTCTCAGAG CTTCCACTCA TGGTCTTGTC ATCAACATCA TTCATTCTCT GTGCACTTGC
    TCACAGCTTC ATTTTAGTGA GGAGACCAAG CAAGTTTTAA GACTGAGCTT GACTGAATTT
    TCTCTGCCTA AATTTTATTT GCTGTTTGGA ATCAGCAAAG TGAAATCAGC TGCAGTCATA
    GCATTCCGCT CCAGCTATCG AGACAGGTCA TTTTCACCTG GCTCCTACGA GAGGGAGACT
    TTTGCACTGA CCTCCTTGGA AACTGTTACT GAAGCTTTGC TGGAGATCAT GGAGGCTTGT
    ATGAGAGATA TTCCAACATG CAAGTGGCTG GACCAGTGGA CCGAACTAGC ACAAAAGTTT
    GCATTCCAGT ACAATCCATC CCTGCAGCCA AGAGCACTCG TTGTCTTTGG CTGTATAAGT
    AAGCGTGTCT CTCATGGACA AATAAAACAA ATAATCCGAA TTCTTAGCAA GGGGCTTGAG
    AGCTGTTTAA AAGGCCCTGA TAATTACAAT AGCCAAGTTC TAATAGAAGC TACGGTTATA
    GCACTCACCA AACTGCAGCC TCTTCTTAAC AAGGACTCCC CCATGCACAA GGCCCTCTTC
    TGGGTAGCCA TGGCAGTACT GCAGTTGGAT GAAGTCAACC TATACTCAGC AGGTACAGCA
    CTGCTTGAAC AGAATCTGCA TACCTTGGAT AGCCTTCGAG TGTTCAATGA TAAGAGCCCA
    GAAGAGGTGT TCATGGAGAT CAGGAGACCA CTTGAATGGC ACTGCAAACA GATGGATCAT
    TTTGTTGGGC TAAATTTCAA CTCCAACTTC AACTTTGCAC TAGTGGGGCA CCTCCTGAAA
    GGGTACAGGC ATCCTTCACC TACCACTGTA GCAAGAACAG TAAGGATTCT ACATACACTA
    CTAGCTCTGG TTAACAAACA CAGGAACTGT GACAAGTTTG AGGTGAACAC CCAGAGCGTG
    GCTTACCTAG CAGCTTTGCT GACAGTGTCT GAGGAAGTTC GAAGTCGCTG CAGCCTAAAA
    CATAGGAAGT CTCTCTTGCT GACAGATGTT GCAATGGAAA ATGTTCCTAT GGATACATAT
    CCCATACATC ACAGTGACAC TACCTATAGG ACACTAAAGG AAAATCAACC ATGGTCATCA
    CCAAAGGGAT CAGACAGACA CCTGGCTGCC AGTTACCCGA CAGTGGGACA GATAAGCCCT
    CGAACACGAA AGTCCATGAG TTTGGATATG GGGCAGCCTT CACAAGCGAA CACTAAAAAG
    CTTCTAGGTA CAAGGAAAAG TTTTGACCAT TTGATATCGG ACACAAAGGC TCCTAAAAGG
    CAAGACATGG AATCTGGAAT CACAACGCCT CCCAAAATGA GGAGAGTAGC CGAAAATGAT
    TATGAAATGG AAACCCAGAG AATATCACCG CAGCAGCACC CCCATCTCCG GAAAGTTTCT
    GTTTCTGAGT CAAATGTCCT CCTGGATGAA GAAGTTCTGA CTGATCCAAA AATTCAAGCA
    CTGCTGCTTA CAGTTCTTGC CACGTTAGTG AAGTACACTA CTGATGAATT TGACCAGCGG
    ATTCTGTATG AGTATTTAGC AGAAGCCAGT GTTGTCTTCC CAAAGGTCTT TCCTGTTGTG
    CATAATTTAT TGGATTCCAA GATCAATACC CTCTTGTCGC TGTGTCAAGA TCCAAATTTG
    TTGAATCCAA TTCATGGGAT TGTTCAGAGT GTTGTCTACC ATGAGGAGTC TCCACCCCAA
    TACCAAACTT CTTATCTACA AAGTTTTGGA TTTAATGGGT TGTGGAGGTT TGCTGGCCCA
    TTTTCCAAGC AAACACAAAT CCCAGACTAT GCTGAGCTCA TAGTGAAGTT TCTTGATGCC
    TTGATTGACA CGTATTTGCC TGGAATTGAT GAGGAAACCA GTGAAGAATC TCTCCTGACA
    CCCACGTCTC CATATCCTCC CGCAGTGCAG AGCCAGCTTA GCATTACAGC TAACCTTAAC
    CTTTCCAATT CCATGACCTC GCTCGCAACT TCCCAGCACT CCCCAGGGAT CGACAAGGAG
    AACGTGGAGC TCTCGCCCAC CACTGGCCAC ACCAACAGCG GGAGGATGCG CCACGGCTCC
    GCCAGCCAGG TGCAAAAGCA GCGAAGTGCT GGCAGCTTGA AACGTCACAG CATTAAGAAG
    ATTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17415
    Clone ID Related Accession (Same CDS sequence) XM_004946785.2 , XM_004946785.3 , XM_025142089.1
    Accession Version XM_004946785.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofibromin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455988.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004946785.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2641
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    4141
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    4441
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    4681
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    5641
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    5881
    5941
    6001
    6061
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    6181
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    6301
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    6421
    6481
    6541
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    7141
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    7621
    7681
    7741
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    7921
    7981
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    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGATGCCAAC CAGTCACTGT TTTGCATGGC AGTGGGCCTC TGTTGCTAGT
    CTGACATTTA AAGAGAAAAT GACAAGCCTT AAATTTAAAG AAAAGCCTAC TGACTTGGAA
    ACCAAAAGCT ACAAGTTCCT GCTGTTATCC TTGGTAAAAC TGATCCATGC TGATCCAAAG
    CTTTTGCTGT GTAATCCAAG GAAGCAAGGG CCTGAGACAC AAGGCAGCAC TGCTGAATTA
    ATTACAGGCC TTGTACAACT CGTTCCACAA TCCAGCATGC CAGATATAGC ACAAGAAGCC
    ATGGAGGCCT TGCTAGTTCT TCACCAGTTA GACAGCATTG ACTTATGGAA TCCTGATGCA
    CCTATAGAAA CTTTCTGGGA AATTAGTTCA CAAATGCTTT TTTATATCTG CAAGAAATTA
    ACCAGTCATC AGATGCTCAG CAGTACAGAA ATCCTCAAGT GGCTGCGAGA GATTCTCATC
    TGTAGGAATA AATTTCTTCT AAAAAATAAA CAATCAGACA GAACTTCCTG TCACTTTCTC
    TTCCTTTATG ATGTCTCTGG AGGTGGAGCT AGTCAGATGT CCCTTGACCA CGAAGAGATG
    ATGCGCTGTG CTCCCGGAGC CACCCTCAGA AAAGGAAAAG GGAATTCTTC CATGGAAAGT
    ACAGCTGGAT GCAGTGGAAC ACCCATTTGT CGCCAGGCCC AAACAAAATT GGAAGTTGCC
    TTATACATGT TTTTGTGGAG TCCGGATATT GAGGCAGTCC TTGTTGCAAT GTCCTGTTTC
    CGTCACCTCT GTGAGGAAGC AGATATCAGA TGTGGAGTAG ATGAAGTTTC AGTTCATAAT
    TTTTTGCCAA ACTACAACAC TTTCATGGAG TTTGCATCTG TTAGCAATAT GATGTCAACA
    GGGAGAGCAG CTCTTCAGAA AAGAGTGATG GCTTTATTAA GACGTATTGA ACATCCAACT
    GCAGGGAACA CAGAGGCTTG GGAGGATACA CATGCAAAAT GGGAACAAGC TACAAAACTA
    ATCCTTAACT ATCCAAAGAC CAAAATGGAA GACGGGCAGG TTACTGAAAG TCTTCATAAG
    ACAATTGTTA AGAGACGAAT GTCACATGTT AGTGGGGGAG GCTCCATAGA CTTGTCTGAC
    ACAGATTCTC TTCAGGAATG GATCAACATG ACGGGATTCC TCTGTGCCCT CGGAGGAGTG
    TGCCTGCAGC ATCGTAGCAA TGCAGGATTA GCAACCTACA GTCCACCCAT GGGCCCAGTC
    AATGAGCGTA AGGGCTCCAT GATATCTATG GTATCCACTG AGGGAAATGC AGAGACTCCT
    GTTAGCAAAT TCATAGATCG ATTACTGACT CTGATGGTCT GTAACCATGA GAAAGTGGGA
    CTACAAATAC GGACTAATAT TAAAGATCTG GTGGGTTTGG AGTTGAGTCC AGCACTTTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGATTC TCAAGGACAG
    GTTTTGTTAA CTGAGACCAA CACACAGTTT GTAGAACAAA CCATTGCTAT AATGAAGAAC
    TTGCTTGACA ATCATACAGA AGGGAGCTCA GAACATCTTG GACAAGCTAG TATTGAGACA
    ATGATGCTAA ACCTGGTTAG GTATGTGCGT GTACTTGGAA ATTTGGTACA TGCAATTCAA
    ATAAAAACTA AACTCTGTCA GTTGGTGGAA GTAATGATGG AGCGGAGAGA TGACCTTTCA
    TTTTGCCAAG AAATGAAATT TAGGAACAAA ATGGTGGAAT ATCTGACAGA CTGGGTTATG
    GGAACATCTA ATCAAGCAAC AGATGAAGAT GTGAAGTGCT TGACAAGAGA TTTGGACCAA
    GCAAGTATGG AAGCAGTGGT CTCTCTTCTT GCTGGGCTTC CGTTACAGCC TGAAGAAGGA
    GATGGTGTTG AGTTGATGGA AGCCAAATCT CAGTTATTTC TTAAATATTT CACACTGTTT
    ATGAACCTTC TAAATGACTG TAGTGAAGTT GAAGATGAAA GTGCACAAAC AGGTGGCAGG
    AAACGTGGGA TGTCCCGAAG ACTGGCTTCG CTGCGGCACT GCACTGTTCT TGCTATGTCA
    AATTTACTAA ATGCAAATGT GGACAGTGGT CTTATGCATT CAATAGGCTT GGGCTATCAT
    AAAGACCTCC AGACAAGAGC TACATTTATG GAAGTCCTCA CCAAAATTCT ACAGCAGGGA
    ACAGAATTTG ATACGTTAGC AGAAACGGTG CTGGCAGATC GGTTTGAGAG GCTGGTGGAG
    CTAGTTACAA TGATGGGTGA TCAGGGGGAG CTTCCTATTG CTATGGCTTT AGCAAATGTG
    GTGCCTTGTT CTCAGTGGGA TGAGCTAGCT CGTGTTCTGG TCACACTCTT TGATTCCCGG
    CACTTGCTCT ACCAGCTACT TTGGAATATG TTTTCAAAGG AAGTTGAACT GGCAGATTCC
    ATGCAGACAC TCTTCCGAGG AAACAGCTTA GCTAGTAAAA TAATGACTTT CTGTTTTAAG
    GTATATGGTG CTACTTATTT GCAGAAGCTT TTGGAACCTT TATTAAGAAC AGTGATCACA
    TCCTCTGAAT GGCAGCATGT TAGCTTTGAG GTGGATCCTA CAAGACTGGA GCCTACAGAA
    AGCCTTGAAG AGAACCAGCG GAGTCTCTTG CAAATGACAG AAAAATTTTT TCATGCAATC
    ATTAATTCTT CTTCGGAATT CCCACCACAA CTTCGCAGTG TGTGCCATTG TTTGTACCAG
    GCAACTTGCC ACTCTCTACT GAATAAAGCT ACCGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTTGTTA GTCAGCGATT TCCTCAGAAC AGCATTGGAG CAGTTGGGAG TGCCATGTTC
    CTGCGGTTCA TCAACCCTGC GATCGTCTCC CCGTACGAGG CAGGGATTTT AGATAAAAAG
    CCTCCGCCCA GAATTGAGAG GGGACTGAAA CTGATGTCAA AGATACTTCA GAGTATTGCC
    AACCACGTCC TGTTTACAAA AGAAGAACAT ATGAGGCCTT TTAATGACTT CGTAAAAAGC
    AATTTTGATG CAGCTCGAAG GTTTTTCCTT GATATTGCAT CTGATTGCCC TGCGAGTGAC
    ACTGTCAATC ACAGCCTGTC TTTCATCAGT GATGGCAATG TTCTTGCTTT GCACCGGTTG
    TTGTGGAACA ATCAGGAGAA AATTGGCCAG TACCTTTCCA GTAATAGGGA CCACAAAGCT
    GTTGGAAGGC GACCTTTTGA CAAAATGGCT ACTCTTCTTG CCTATCTGGG TCCACCAGAG
    CACAAACCTG TGGCAGATAC ACATTGGTCC AGTTTAAACC TCACCAGTTC GAAATTTGAA
    GAATTTATGA CAAGGCATCA GGTACATGAA AAAGAAGAGT TCAAAGCATT GAAAACGTTA
    AATATTTTCT ACCAGGCTGG GACATCCAAA GCTGGCAATC CTGTTTTCTA CTACATTGCT
    CGGCGTTTCA AGACTGGTCA GATCAATGGA GATTTATTGA TATACCACGT CCTGCTAACT
    CTGAAGCCAT ACTACGCAAA GCCATATGAA ATTGTAGTGG ACCTCACACA TGCTGGCCCC
    AGTAATCGCT TTAAAACAGA CTTTCTTTCT AAATGGTTTG TAGTTTTTCC AGGCTTTGCT
    TATGAAAATG TCTCAGCAGT TTTTATTTAT AACTGTAATT CTTGGGTCAG AGAATACACC
    AAATACCACG AAAGACTCTT GACAGGTTTG AAAGGAAGCA AAAGGCTTAT TTTCATAGAC
    TCTCCAGGAA AACTGGCGGA GCACATTGAA CATGACCAGC AGAAACTTCC AGCAGCTACC
    TTAGCTTTGG AAGAGGACTT GAAAGTATTT CACAATGCCC TCAAATTGGC TCACAAAGAC
    ACCAAAGTGT CTATTAAAGT TGGATCAACA GCTGTGCAGG TGACATCAGC AGAACGAACA
    AGAGTCCTGG GACAGACAGT GTTTTTAAAT GATATATACT ACGCCTCTGA AATTGAGGAA
    ATATGTCTTG TTGATGAGAA TCAGTTTACA TTGACGATTG CCAACCAGGG AACGCCACTC
    ACCTTCATGC ATCAGGAGTG TGAAGCCATT GTGAGGTCCA TTATTCATAT ACGGACACGG
    TGGGAGCTGT CGCAACCAGA CTCCATCCCA CAGCACACTA AAATTCGCCC TAAGGATGTT
    CCCGGAACAC TACTGAACAT AGCATTGCTC AACCTGGGAA GCTCAGACCC GAGTTTGCGG
    TCTGCTGCCT ATAATCTTCT GTGTGCCTTA ACCTGTACCT TTAATTTAAA GATTGAGGGC
    CAGTTACTGG AAACTTCAGG TCTGTGTATT CCTGCCAACA ACACGCTCTT TATTGTGTCT
    ATTAGTAAGA CTCTTGCAGC TAATGAGCCA CACCTTACCC TAGAGTTCTT GGAAGAGTGT
    ATTTCAGGAT TTAGCAAATC TAGTATTGAA CTGAAACATC TTTGTCTGGA ATATATGACT
    CCCTGGCTGT CCAATCTGGT CCGATTCTGC AAACACAACG ACGATGCGAA GCGCCAGCGA
    GTCACTGCCA TTCTTGATAA GCTTATAACA ATGACAATAA ATGAAAAACA GATGTACCCT
    TCTATTCAGG CTAAAATATG GGGAAGTCTT GGACAGATTA CAGATCTCCT TGACGTAGTG
    CTGGACAGTT TCATCAAAAC TAGTGCCACG GGGGGCTTAG GATCCATTAA AGCTGAGGTT
    ATGGCAGACA CAGCAGTAGC GCTTGCTTCT GGAAATGTAA AATTGGTTTC AAGCAAAGTG
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACTTGTCTGT CTCCAACTCC TACACTGGAA
    CAGCACCTGA TGTGGGATGA TATTGCCATT CTAGCACGTT ACATGCTGAT GCTCTCCTTC
    AACAATTCTC TGGATGTGGC TGCACATCTC CCCTACCTCT TTCATGTGGT TACTTTGTTG
    GTGGCCACTG GACCCCTTTC TCTCAGAGCT TCCACTCATG GTCTTGTCAT CAACATCATT
    CATTCTCTGT GCACTTGCTC ACAGCTTCAT TTTAGTGAGG AGACCAAGCA AGTTTTAAGA
    CTGAGCTTGA CTGAATTTTC TCTGCCTAAA TTTTATTTGC TGTTTGGAAT CAGCAAAGTG
    AAATCAGCTG CAGTCATAGC ATTCCGCTCC AGCTATCGAG ACAGGTCATT TTCACCTGGC
    TCCTACGAGA GGGAGACTTT TGCACTGACC TCCTTGGAAA CTGTTACTGA AGCTTTGCTG
    GAGATCATGG AGGCTTGTAT GAGAGATATT CCAACATGCA AGTGGCTGGA CCAGTGGACC
    GAACTAGCAC AAAAGTTTGC ATTCCAGTAC AATCCATCCC TGCAGCCAAG AGCACTCGTT
    GTCTTTGGCT GTATAAGTAA GCGTGTCTCT CATGGACAAA TAAAACAAAT AATCCGAATT
    CTTAGCAAGG GGCTTGAGAG CTGTTTAAAA GGCCCTGATA ATTACAATAG CCAAGTTCTA
    ATAGAAGCTA CGGTTATAGC ACTCACCAAA CTGCAGCCTC TTCTTAACAA GGACTCCCCC
    ATGCACAAGG CCCTCTTCTG GGTAGCCATG GCAGTACTGC AGTTGGATGA AGTCAACCTA
    TACTCAGCAG GTACAGCACT GCTTGAACAG AATCTGCATA CCTTGGATAG CCTTCGAGTG
    TTCAATGATA AGAGCCCAGA AGAGGTGTTC ATGGAGATCA GGAGACCACT TGAATGGCAC
    TGCAAACAGA TGGATCATTT TGTTGGGCTA AATTTCAACT CCAACTTCAA CTTTGCACTA
    GTGGGGCACC TCCTGAAAGG GTACAGGCAT CCTTCACCTA CCACTGTAGC AAGAACAGTA
    AGGATTCTAC ATACACTACT AGCTCTGGTT AACAAACACA GGAACTGTGA CAAGTTTGAG
    GTGAACACCC AGAGCGTGGC TTACCTAGCA GCTTTGCTGA CAGTGTCTGA GGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGGAAGTCT CTCTTGCTGA CAGATGTTGC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATACATCAC AGTGACACTA CCTATAGGAC ACTAAAGGAA
    AATCAACCAT GGTCATCACC AAAGGGATCA GACAGACACC TGGCTGCCAG TTACCCGACA
    GTGGGACAGA TAAGCCCTCG AACACGAAAG TCCATGAGTT TGGATATGGG GCAGCCTTCA
    CAAGCGAACA CTAAAAAGCT TCTAGGTACA AGGAAAAGTT TTGACCATTT GATATCGGAC
    ACAAAGGCTC CTAAAAGGCA AGACATGGAA TCTGGAATCA CAACGCCTCC CAAAATGAGG
    AGAGTAGCCG AAAATGATTA TGAAATGGAA ACCCAGAGAA TATCACCGCA GCAGCACCCC
    CATCTCCGGA AAGTTTCTGT TTCTGAGTCA AATGTCCTCC TGGATGAAGA AGTTCTGACT
    GATCCAAAAA TTCAAGCACT GCTGCTTACA GTTCTTGCCA CGTTAGTGAA GTACACTACT
    GATGAATTTG ACCAGCGGAT TCTGTATGAG TATTTAGCAG AAGCCAGTGT TGTCTTCCCA
    AAGGTCTTTC CTGTTGTGCA TAATTTATTG GATTCCAAGA TCAATACCCT CTTGTCGCTG
    TGTCAAGATC CAAATTTGTT GAATCCAATT CATGGGATTG TTCAGAGTGT TGTCTACCAT
    GAGGAGTCTC CACCCCAATA CCAAACTTCT TATCTACAAA GTTTTGGATT TAATGGGTTG
    TGGAGGTTTG CTGGCCCATT TTCCAAGCAA ACACAAATCC CAGACTATGC TGAGCTCATA
    GTGAAGTTTC TTGATGCCTT GATTGACACG TATTTGCCTG GAATTGATGA GGAAACCAGT
    GAAGAATCTC TCCTGACACC CACGTCTCCA TATCCTCCCG CAGTGCAGAG CCAGCTTAGC
    ATTACAGCTA ACCTTAACCT TTCCAATTCC ATGACCTCGC TCGCAACTTC CCAGCACTCC
    CCAGGGATCG ACAAGGAGAA CGTGGAGCTC TCGCCCACCA CTGGCCACAC CAACAGCGGG
    AGGATGCGCC ACGGCTCCGC CAGCCAGGTG CAAAAGCAGC GAAGTGCTGG CAGCTTGAAA
    CGTCACAGCA TTAAGAAGAT TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004946842.1
    CDS215..8761
    Translation

    Target ORF information:

    RefSeq Version XM_004946785.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofibromin 1 (NF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004946785.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGATGCCAAC CAGTCACTGT TTTGCATGGC AGTGGGCCTC TGTTGCTAGT
    CTGACATTTA AAGAGAAAAT GACAAGCCTT AAATTTAAAG AAAAGCCTAC TGACTTGGAA
    ACCAAAAGCT ACAAGTTCCT GCTGTTATCC TTGGTAAAAC TGATCCATGC TGATCCAAAG
    CTTTTGCTGT GTAATCCAAG GAAGCAAGGG CCTGAGACAC AAGGCAGCAC TGCTGAATTA
    ATTACAGGCC TTGTACAACT CGTTCCACAA TCCAGCATGC CAGATATAGC ACAAGAAGCC
    ATGGAGGCCT TGCTAGTTCT TCACCAGTTA GACAGCATTG ACTTATGGAA TCCTGATGCA
    CCTATAGAAA CTTTCTGGGA AATTAGTTCA CAAATGCTTT TTTATATCTG CAAGAAATTA
    ACCAGTCATC AGATGCTCAG CAGTACAGAA ATCCTCAAGT GGCTGCGAGA GATTCTCATC
    TGTAGGAATA AATTTCTTCT AAAAAATAAA CAATCAGACA GAACTTCCTG TCACTTTCTC
    TTCCTTTATG ATGTCTCTGG AGGTGGAGCT AGTCAGATGT CCCTTGACCA CGAAGAGATG
    ATGCGCTGTG CTCCCGGAGC CACCCTCAGA AAAGGAAAAG GGAATTCTTC CATGGAAAGT
    ACAGCTGGAT GCAGTGGAAC ACCCATTTGT CGCCAGGCCC AAACAAAATT GGAAGTTGCC
    TTATACATGT TTTTGTGGAG TCCGGATATT GAGGCAGTCC TTGTTGCAAT GTCCTGTTTC
    CGTCACCTCT GTGAGGAAGC AGATATCAGA TGTGGAGTAG ATGAAGTTTC AGTTCATAAT
    TTTTTGCCAA ACTACAACAC TTTCATGGAG TTTGCATCTG TTAGCAATAT GATGTCAACA
    GGGAGAGCAG CTCTTCAGAA AAGAGTGATG GCTTTATTAA GACGTATTGA ACATCCAACT
    GCAGGGAACA CAGAGGCTTG GGAGGATACA CATGCAAAAT GGGAACAAGC TACAAAACTA
    ATCCTTAACT ATCCAAAGAC CAAAATGGAA GACGGGCAGG TTACTGAAAG TCTTCATAAG
    ACAATTGTTA AGAGACGAAT GTCACATGTT AGTGGGGGAG GCTCCATAGA CTTGTCTGAC
    ACAGATTCTC TTCAGGAATG GATCAACATG ACGGGATTCC TCTGTGCCCT CGGAGGAGTG
    TGCCTGCAGC ATCGTAGCAA TGCAGGATTA GCAACCTACA GTCCACCCAT GGGCCCAGTC
    AATGAGCGTA AGGGCTCCAT GATATCTATG GTATCCACTG AGGGAAATGC AGAGACTCCT
    GTTAGCAAAT TCATAGATCG ATTACTGACT CTGATGGTCT GTAACCATGA GAAAGTGGGA
    CTACAAATAC GGACTAATAT TAAAGATCTG GTGGGTTTGG AGTTGAGTCC AGCACTTTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGATTC TCAAGGACAG
    GTTTTGTTAA CTGAGACCAA CACACAGTTT GTAGAACAAA CCATTGCTAT AATGAAGAAC
    TTGCTTGACA ATCATACAGA AGGGAGCTCA GAACATCTTG GACAAGCTAG TATTGAGACA
    ATGATGCTAA ACCTGGTTAG GTATGTGCGT GTACTTGGAA ATTTGGTACA TGCAATTCAA
    ATAAAAACTA AACTCTGTCA GTTGGTGGAA GTAATGATGG AGCGGAGAGA TGACCTTTCA
    TTTTGCCAAG AAATGAAATT TAGGAACAAA ATGGTGGAAT ATCTGACAGA CTGGGTTATG
    GGAACATCTA ATCAAGCAAC AGATGAAGAT GTGAAGTGCT TGACAAGAGA TTTGGACCAA
    GCAAGTATGG AAGCAGTGGT CTCTCTTCTT GCTGGGCTTC CGTTACAGCC TGAAGAAGGA
    GATGGTGTTG AGTTGATGGA AGCCAAATCT CAGTTATTTC TTAAATATTT CACACTGTTT
    ATGAACCTTC TAAATGACTG TAGTGAAGTT GAAGATGAAA GTGCACAAAC AGGTGGCAGG
    AAACGTGGGA TGTCCCGAAG ACTGGCTTCG CTGCGGCACT GCACTGTTCT TGCTATGTCA
    AATTTACTAA ATGCAAATGT GGACAGTGGT CTTATGCATT CAATAGGCTT GGGCTATCAT
    AAAGACCTCC AGACAAGAGC TACATTTATG GAAGTCCTCA CCAAAATTCT ACAGCAGGGA
    ACAGAATTTG ATACGTTAGC AGAAACGGTG CTGGCAGATC GGTTTGAGAG GCTGGTGGAG
    CTAGTTACAA TGATGGGTGA TCAGGGGGAG CTTCCTATTG CTATGGCTTT AGCAAATGTG
    GTGCCTTGTT CTCAGTGGGA TGAGCTAGCT CGTGTTCTGG TCACACTCTT TGATTCCCGG
    CACTTGCTCT ACCAGCTACT TTGGAATATG TTTTCAAAGG AAGTTGAACT GGCAGATTCC
    ATGCAGACAC TCTTCCGAGG AAACAGCTTA GCTAGTAAAA TAATGACTTT CTGTTTTAAG
    GTATATGGTG CTACTTATTT GCAGAAGCTT TTGGAACCTT TATTAAGAAC AGTGATCACA
    TCCTCTGAAT GGCAGCATGT TAGCTTTGAG GTGGATCCTA CAAGACTGGA GCCTACAGAA
    AGCCTTGAAG AGAACCAGCG GAGTCTCTTG CAAATGACAG AAAAATTTTT TCATGCAATC
    ATTAATTCTT CTTCGGAATT CCCACCACAA CTTCGCAGTG TGTGCCATTG TTTGTACCAG
    GCAACTTGCC ACTCTCTACT GAATAAAGCT ACCGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTTGTTA GTCAGCGATT TCCTCAGAAC AGCATTGGAG CAGTTGGGAG TGCCATGTTC
    CTGCGGTTCA TCAACCCTGC GATCGTCTCC CCGTACGAGG CAGGGATTTT AGATAAAAAG
    CCTCCGCCCA GAATTGAGAG GGGACTGAAA CTGATGTCAA AGATACTTCA GAGTATTGCC
    AACCACGTCC TGTTTACAAA AGAAGAACAT ATGAGGCCTT TTAATGACTT CGTAAAAAGC
    AATTTTGATG CAGCTCGAAG GTTTTTCCTT GATATTGCAT CTGATTGCCC TGCGAGTGAC
    ACTGTCAATC ACAGCCTGTC TTTCATCAGT GATGGCAATG TTCTTGCTTT GCACCGGTTG
    TTGTGGAACA ATCAGGAGAA AATTGGCCAG TACCTTTCCA GTAATAGGGA CCACAAAGCT
    GTTGGAAGGC GACCTTTTGA CAAAATGGCT ACTCTTCTTG CCTATCTGGG TCCACCAGAG
    CACAAACCTG TGGCAGATAC ACATTGGTCC AGTTTAAACC TCACCAGTTC GAAATTTGAA
    GAATTTATGA CAAGGCATCA GGTACATGAA AAAGAAGAGT TCAAAGCATT GAAAACGTTA
    AATATTTTCT ACCAGGCTGG GACATCCAAA GCTGGCAATC CTGTTTTCTA CTACATTGCT
    CGGCGTTTCA AGACTGGTCA GATCAATGGA GATTTATTGA TATACCACGT CCTGCTAACT
    CTGAAGCCAT ACTACGCAAA GCCATATGAA ATTGTAGTGG ACCTCACACA TGCTGGCCCC
    AGTAATCGCT TTAAAACAGA CTTTCTTTCT AAATGGTTTG TAGTTTTTCC AGGCTTTGCT
    TATGAAAATG TCTCAGCAGT TTTTATTTAT AACTGTAATT CTTGGGTCAG AGAATACACC
    AAATACCACG AAAGACTCTT GACAGGTTTG AAAGGAAGCA AAAGGCTTAT TTTCATAGAC
    TCTCCAGGAA AACTGGCGGA GCACATTGAA CATGACCAGC AGAAACTTCC AGCAGCTACC
    TTAGCTTTGG AAGAGGACTT GAAAGTATTT CACAATGCCC TCAAATTGGC TCACAAAGAC
    ACCAAAGTGT CTATTAAAGT TGGATCAACA GCTGTGCAGG TGACATCAGC AGAACGAACA
    AGAGTCCTGG GACAGACAGT GTTTTTAAAT GATATATACT ACGCCTCTGA AATTGAGGAA
    ATATGTCTTG TTGATGAGAA TCAGTTTACA TTGACGATTG CCAACCAGGG AACGCCACTC
    ACCTTCATGC ATCAGGAGTG TGAAGCCATT GTGAGGTCCA TTATTCATAT ACGGACACGG
    TGGGAGCTGT CGCAACCAGA CTCCATCCCA CAGCACACTA AAATTCGCCC TAAGGATGTT
    CCCGGAACAC TACTGAACAT AGCATTGCTC AACCTGGGAA GCTCAGACCC GAGTTTGCGG
    TCTGCTGCCT ATAATCTTCT GTGTGCCTTA ACCTGTACCT TTAATTTAAA GATTGAGGGC
    CAGTTACTGG AAACTTCAGG TCTGTGTATT CCTGCCAACA ACACGCTCTT TATTGTGTCT
    ATTAGTAAGA CTCTTGCAGC TAATGAGCCA CACCTTACCC TAGAGTTCTT GGAAGAGTGT
    ATTTCAGGAT TTAGCAAATC TAGTATTGAA CTGAAACATC TTTGTCTGGA ATATATGACT
    CCCTGGCTGT CCAATCTGGT CCGATTCTGC AAACACAACG ACGATGCGAA GCGCCAGCGA
    GTCACTGCCA TTCTTGATAA GCTTATAACA ATGACAATAA ATGAAAAACA GATGTACCCT
    TCTATTCAGG CTAAAATATG GGGAAGTCTT GGACAGATTA CAGATCTCCT TGACGTAGTG
    CTGGACAGTT TCATCAAAAC TAGTGCCACG GGGGGCTTAG GATCCATTAA AGCTGAGGTT
    ATGGCAGACA CAGCAGTAGC GCTTGCTTCT GGAAATGTAA AATTGGTTTC AAGCAAAGTG
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACTTGTCTGT CTCCAACTCC TACACTGGAA
    CAGCACCTGA TGTGGGATGA TATTGCCATT CTAGCACGTT ACATGCTGAT GCTCTCCTTC
    AACAATTCTC TGGATGTGGC TGCACATCTC CCCTACCTCT TTCATGTGGT TACTTTGTTG
    GTGGCCACTG GACCCCTTTC TCTCAGAGCT TCCACTCATG GTCTTGTCAT CAACATCATT
    CATTCTCTGT GCACTTGCTC ACAGCTTCAT TTTAGTGAGG AGACCAAGCA AGTTTTAAGA
    CTGAGCTTGA CTGAATTTTC TCTGCCTAAA TTTTATTTGC TGTTTGGAAT CAGCAAAGTG
    AAATCAGCTG CAGTCATAGC ATTCCGCTCC AGCTATCGAG ACAGGTCATT TTCACCTGGC
    TCCTACGAGA GGGAGACTTT TGCACTGACC TCCTTGGAAA CTGTTACTGA AGCTTTGCTG
    GAGATCATGG AGGCTTGTAT GAGAGATATT CCAACATGCA AGTGGCTGGA CCAGTGGACC
    GAACTAGCAC AAAAGTTTGC ATTCCAGTAC AATCCATCCC TGCAGCCAAG AGCACTCGTT
    GTCTTTGGCT GTATAAGTAA GCGTGTCTCT CATGGACAAA TAAAACAAAT AATCCGAATT
    CTTAGCAAGG GGCTTGAGAG CTGTTTAAAA GGCCCTGATA ATTACAATAG CCAAGTTCTA
    ATAGAAGCTA CGGTTATAGC ACTCACCAAA CTGCAGCCTC TTCTTAACAA GGACTCCCCC
    ATGCACAAGG CCCTCTTCTG GGTAGCCATG GCAGTACTGC AGTTGGATGA AGTCAACCTA
    TACTCAGCAG GTACAGCACT GCTTGAACAG AATCTGCATA CCTTGGATAG CCTTCGAGTG
    TTCAATGATA AGAGCCCAGA AGAGGTGTTC ATGGAGATCA GGAGACCACT TGAATGGCAC
    TGCAAACAGA TGGATCATTT TGTTGGGCTA AATTTCAACT CCAACTTCAA CTTTGCACTA
    GTGGGGCACC TCCTGAAAGG GTACAGGCAT CCTTCACCTA CCACTGTAGC AAGAACAGTA
    AGGATTCTAC ATACACTACT AGCTCTGGTT AACAAACACA GGAACTGTGA CAAGTTTGAG
    GTGAACACCC AGAGCGTGGC TTACCTAGCA GCTTTGCTGA CAGTGTCTGA GGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGGAAGTCT CTCTTGCTGA CAGATGTTGC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATACATCAC AGTGACACTA CCTATAGGAC ACTAAAGGAA
    AATCAACCAT GGTCATCACC AAAGGGATCA GACAGACACC TGGCTGCCAG TTACCCGACA
    GTGGGACAGA TAAGCCCTCG AACACGAAAG TCCATGAGTT TGGATATGGG GCAGCCTTCA
    CAAGCGAACA CTAAAAAGCT TCTAGGTACA AGGAAAAGTT TTGACCATTT GATATCGGAC
    ACAAAGGCTC CTAAAAGGCA AGACATGGAA TCTGGAATCA CAACGCCTCC CAAAATGAGG
    AGAGTAGCCG AAAATGATTA TGAAATGGAA ACCCAGAGAA TATCACCGCA GCAGCACCCC
    CATCTCCGGA AAGTTTCTGT TTCTGAGTCA AATGTCCTCC TGGATGAAGA AGTTCTGACT
    GATCCAAAAA TTCAAGCACT GCTGCTTACA GTTCTTGCCA CGTTAGTGAA GTACACTACT
    GATGAATTTG ACCAGCGGAT TCTGTATGAG TATTTAGCAG AAGCCAGTGT TGTCTTCCCA
    AAGGTCTTTC CTGTTGTGCA TAATTTATTG GATTCCAAGA TCAATACCCT CTTGTCGCTG
    TGTCAAGATC CAAATTTGTT GAATCCAATT CATGGGATTG TTCAGAGTGT TGTCTACCAT
    GAGGAGTCTC CACCCCAATA CCAAACTTCT TATCTACAAA GTTTTGGATT TAATGGGTTG
    TGGAGGTTTG CTGGCCCATT TTCCAAGCAA ACACAAATCC CAGACTATGC TGAGCTCATA
    GTGAAGTTTC TTGATGCCTT GATTGACACG TATTTGCCTG GAATTGATGA GGAAACCAGT
    GAAGAATCTC TCCTGACACC CACGTCTCCA TATCCTCCCG CAGTGCAGAG CCAGCTTAGC
    ATTACAGCTA ACCTTAACCT TTCCAATTCC ATGACCTCGC TCGCAACTTC CCAGCACTCC
    CCAGGGATCG ACAAGGAGAA CGTGGAGCTC TCGCCCACCA CTGGCCACAC CAACAGCGGG
    AGGATGCGCC ACGGCTCCGC CAGCCAGGTG CAAAAGCAGC GAAGTGCTGG CAGCTTGAAA
    CGTCACAGCA TTAAGAAGAT TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17415
    Clone ID Related Accession (Same CDS sequence) XM_004946785.2 , XM_004946785.3 , XM_025142089.1
    Accession Version XM_004946785.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofibromin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004946785.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGATGCCAAC CAGTCACTGT TTTGCATGGC AGTGGGCCTC TGTTGCTAGT
    CTGACATTTA AAGAGAAAAT GACAAGCCTT AAATTTAAAG AAAAGCCTAC TGACTTGGAA
    ACCAAAAGCT ACAAGTTCCT GCTGTTATCC TTGGTAAAAC TGATCCATGC TGATCCAAAG
    CTTTTGCTGT GTAATCCAAG GAAGCAAGGG CCTGAGACAC AAGGCAGCAC TGCTGAATTA
    ATTACAGGCC TTGTACAACT CGTTCCACAA TCCAGCATGC CAGATATAGC ACAAGAAGCC
    ATGGAGGCCT TGCTAGTTCT TCACCAGTTA GACAGCATTG ACTTATGGAA TCCTGATGCA
    CCTATAGAAA CTTTCTGGGA AATTAGTTCA CAAATGCTTT TTTATATCTG CAAGAAATTA
    ACCAGTCATC AGATGCTCAG CAGTACAGAA ATCCTCAAGT GGCTGCGAGA GATTCTCATC
    TGTAGGAATA AATTTCTTCT AAAAAATAAA CAATCAGACA GAACTTCCTG TCACTTTCTC
    TTCCTTTATG ATGTCTCTGG AGGTGGAGCT AGTCAGATGT CCCTTGACCA CGAAGAGATG
    ATGCGCTGTG CTCCCGGAGC CACCCTCAGA AAAGGAAAAG GGAATTCTTC CATGGAAAGT
    ACAGCTGGAT GCAGTGGAAC ACCCATTTGT CGCCAGGCCC AAACAAAATT GGAAGTTGCC
    TTATACATGT TTTTGTGGAG TCCGGATATT GAGGCAGTCC TTGTTGCAAT GTCCTGTTTC
    CGTCACCTCT GTGAGGAAGC AGATATCAGA TGTGGAGTAG ATGAAGTTTC AGTTCATAAT
    TTTTTGCCAA ACTACAACAC TTTCATGGAG TTTGCATCTG TTAGCAATAT GATGTCAACA
    GGGAGAGCAG CTCTTCAGAA AAGAGTGATG GCTTTATTAA GACGTATTGA ACATCCAACT
    GCAGGGAACA CAGAGGCTTG GGAGGATACA CATGCAAAAT GGGAACAAGC TACAAAACTA
    ATCCTTAACT ATCCAAAGAC CAAAATGGAA GACGGGCAGG TTACTGAAAG TCTTCATAAG
    ACAATTGTTA AGAGACGAAT GTCACATGTT AGTGGGGGAG GCTCCATAGA CTTGTCTGAC
    ACAGATTCTC TTCAGGAATG GATCAACATG ACGGGATTCC TCTGTGCCCT CGGAGGAGTG
    TGCCTGCAGC ATCGTAGCAA TGCAGGATTA GCAACCTACA GTCCACCCAT GGGCCCAGTC
    AATGAGCGTA AGGGCTCCAT GATATCTATG GTATCCACTG AGGGAAATGC AGAGACTCCT
    GTTAGCAAAT TCATAGATCG ATTACTGACT CTGATGGTCT GTAACCATGA GAAAGTGGGA
    CTACAAATAC GGACTAATAT TAAAGATCTG GTGGGTTTGG AGTTGAGTCC AGCACTTTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGATTC TCAAGGACAG
    GTTTTGTTAA CTGAGACCAA CACACAGTTT GTAGAACAAA CCATTGCTAT AATGAAGAAC
    TTGCTTGACA ATCATACAGA AGGGAGCTCA GAACATCTTG GACAAGCTAG TATTGAGACA
    ATGATGCTAA ACCTGGTTAG GTATGTGCGT GTACTTGGAA ATTTGGTACA TGCAATTCAA
    ATAAAAACTA AACTCTGTCA GTTGGTGGAA GTAATGATGG AGCGGAGAGA TGACCTTTCA
    TTTTGCCAAG AAATGAAATT TAGGAACAAA ATGGTGGAAT ATCTGACAGA CTGGGTTATG
    GGAACATCTA ATCAAGCAAC AGATGAAGAT GTGAAGTGCT TGACAAGAGA TTTGGACCAA
    GCAAGTATGG AAGCAGTGGT CTCTCTTCTT GCTGGGCTTC CGTTACAGCC TGAAGAAGGA
    GATGGTGTTG AGTTGATGGA AGCCAAATCT CAGTTATTTC TTAAATATTT CACACTGTTT
    ATGAACCTTC TAAATGACTG TAGTGAAGTT GAAGATGAAA GTGCACAAAC AGGTGGCAGG
    AAACGTGGGA TGTCCCGAAG ACTGGCTTCG CTGCGGCACT GCACTGTTCT TGCTATGTCA
    AATTTACTAA ATGCAAATGT GGACAGTGGT CTTATGCATT CAATAGGCTT GGGCTATCAT
    AAAGACCTCC AGACAAGAGC TACATTTATG GAAGTCCTCA CCAAAATTCT ACAGCAGGGA
    ACAGAATTTG ATACGTTAGC AGAAACGGTG CTGGCAGATC GGTTTGAGAG GCTGGTGGAG
    CTAGTTACAA TGATGGGTGA TCAGGGGGAG CTTCCTATTG CTATGGCTTT AGCAAATGTG
    GTGCCTTGTT CTCAGTGGGA TGAGCTAGCT CGTGTTCTGG TCACACTCTT TGATTCCCGG
    CACTTGCTCT ACCAGCTACT TTGGAATATG TTTTCAAAGG AAGTTGAACT GGCAGATTCC
    ATGCAGACAC TCTTCCGAGG AAACAGCTTA GCTAGTAAAA TAATGACTTT CTGTTTTAAG
    GTATATGGTG CTACTTATTT GCAGAAGCTT TTGGAACCTT TATTAAGAAC AGTGATCACA
    TCCTCTGAAT GGCAGCATGT TAGCTTTGAG GTGGATCCTA CAAGACTGGA GCCTACAGAA
    AGCCTTGAAG AGAACCAGCG GAGTCTCTTG CAAATGACAG AAAAATTTTT TCATGCAATC
    ATTAATTCTT CTTCGGAATT CCCACCACAA CTTCGCAGTG TGTGCCATTG TTTGTACCAG
    GCAACTTGCC ACTCTCTACT GAATAAAGCT ACCGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTTGTTA GTCAGCGATT TCCTCAGAAC AGCATTGGAG CAGTTGGGAG TGCCATGTTC
    CTGCGGTTCA TCAACCCTGC GATCGTCTCC CCGTACGAGG CAGGGATTTT AGATAAAAAG
    CCTCCGCCCA GAATTGAGAG GGGACTGAAA CTGATGTCAA AGATACTTCA GAGTATTGCC
    AACCACGTCC TGTTTACAAA AGAAGAACAT ATGAGGCCTT TTAATGACTT CGTAAAAAGC
    AATTTTGATG CAGCTCGAAG GTTTTTCCTT GATATTGCAT CTGATTGCCC TGCGAGTGAC
    ACTGTCAATC ACAGCCTGTC TTTCATCAGT GATGGCAATG TTCTTGCTTT GCACCGGTTG
    TTGTGGAACA ATCAGGAGAA AATTGGCCAG TACCTTTCCA GTAATAGGGA CCACAAAGCT
    GTTGGAAGGC GACCTTTTGA CAAAATGGCT ACTCTTCTTG CCTATCTGGG TCCACCAGAG
    CACAAACCTG TGGCAGATAC ACATTGGTCC AGTTTAAACC TCACCAGTTC GAAATTTGAA
    GAATTTATGA CAAGGCATCA GGTACATGAA AAAGAAGAGT TCAAAGCATT GAAAACGTTA
    AATATTTTCT ACCAGGCTGG GACATCCAAA GCTGGCAATC CTGTTTTCTA CTACATTGCT
    CGGCGTTTCA AGACTGGTCA GATCAATGGA GATTTATTGA TATACCACGT CCTGCTAACT
    CTGAAGCCAT ACTACGCAAA GCCATATGAA ATTGTAGTGG ACCTCACACA TGCTGGCCCC
    AGTAATCGCT TTAAAACAGA CTTTCTTTCT AAATGGTTTG TAGTTTTTCC AGGCTTTGCT
    TATGAAAATG TCTCAGCAGT TTTTATTTAT AACTGTAATT CTTGGGTCAG AGAATACACC
    AAATACCACG AAAGACTCTT GACAGGTTTG AAAGGAAGCA AAAGGCTTAT TTTCATAGAC
    TCTCCAGGAA AACTGGCGGA GCACATTGAA CATGACCAGC AGAAACTTCC AGCAGCTACC
    TTAGCTTTGG AAGAGGACTT GAAAGTATTT CACAATGCCC TCAAATTGGC TCACAAAGAC
    ACCAAAGTGT CTATTAAAGT TGGATCAACA GCTGTGCAGG TGACATCAGC AGAACGAACA
    AGAGTCCTGG GACAGACAGT GTTTTTAAAT GATATATACT ACGCCTCTGA AATTGAGGAA
    ATATGTCTTG TTGATGAGAA TCAGTTTACA TTGACGATTG CCAACCAGGG AACGCCACTC
    ACCTTCATGC ATCAGGAGTG TGAAGCCATT GTGAGGTCCA TTATTCATAT ACGGACACGG
    TGGGAGCTGT CGCAACCAGA CTCCATCCCA CAGCACACTA AAATTCGCCC TAAGGATGTT
    CCCGGAACAC TACTGAACAT AGCATTGCTC AACCTGGGAA GCTCAGACCC GAGTTTGCGG
    TCTGCTGCCT ATAATCTTCT GTGTGCCTTA ACCTGTACCT TTAATTTAAA GATTGAGGGC
    CAGTTACTGG AAACTTCAGG TCTGTGTATT CCTGCCAACA ACACGCTCTT TATTGTGTCT
    ATTAGTAAGA CTCTTGCAGC TAATGAGCCA CACCTTACCC TAGAGTTCTT GGAAGAGTGT
    ATTTCAGGAT TTAGCAAATC TAGTATTGAA CTGAAACATC TTTGTCTGGA ATATATGACT
    CCCTGGCTGT CCAATCTGGT CCGATTCTGC AAACACAACG ACGATGCGAA GCGCCAGCGA
    GTCACTGCCA TTCTTGATAA GCTTATAACA ATGACAATAA ATGAAAAACA GATGTACCCT
    TCTATTCAGG CTAAAATATG GGGAAGTCTT GGACAGATTA CAGATCTCCT TGACGTAGTG
    CTGGACAGTT TCATCAAAAC TAGTGCCACG GGGGGCTTAG GATCCATTAA AGCTGAGGTT
    ATGGCAGACA CAGCAGTAGC GCTTGCTTCT GGAAATGTAA AATTGGTTTC AAGCAAAGTG
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACTTGTCTGT CTCCAACTCC TACACTGGAA
    CAGCACCTGA TGTGGGATGA TATTGCCATT CTAGCACGTT ACATGCTGAT GCTCTCCTTC
    AACAATTCTC TGGATGTGGC TGCACATCTC CCCTACCTCT TTCATGTGGT TACTTTGTTG
    GTGGCCACTG GACCCCTTTC TCTCAGAGCT TCCACTCATG GTCTTGTCAT CAACATCATT
    CATTCTCTGT GCACTTGCTC ACAGCTTCAT TTTAGTGAGG AGACCAAGCA AGTTTTAAGA
    CTGAGCTTGA CTGAATTTTC TCTGCCTAAA TTTTATTTGC TGTTTGGAAT CAGCAAAGTG
    AAATCAGCTG CAGTCATAGC ATTCCGCTCC AGCTATCGAG ACAGGTCATT TTCACCTGGC
    TCCTACGAGA GGGAGACTTT TGCACTGACC TCCTTGGAAA CTGTTACTGA AGCTTTGCTG
    GAGATCATGG AGGCTTGTAT GAGAGATATT CCAACATGCA AGTGGCTGGA CCAGTGGACC
    GAACTAGCAC AAAAGTTTGC ATTCCAGTAC AATCCATCCC TGCAGCCAAG AGCACTCGTT
    GTCTTTGGCT GTATAAGTAA GCGTGTCTCT CATGGACAAA TAAAACAAAT AATCCGAATT
    CTTAGCAAGG GGCTTGAGAG CTGTTTAAAA GGCCCTGATA ATTACAATAG CCAAGTTCTA
    ATAGAAGCTA CGGTTATAGC ACTCACCAAA CTGCAGCCTC TTCTTAACAA GGACTCCCCC
    ATGCACAAGG CCCTCTTCTG GGTAGCCATG GCAGTACTGC AGTTGGATGA AGTCAACCTA
    TACTCAGCAG GTACAGCACT GCTTGAACAG AATCTGCATA CCTTGGATAG CCTTCGAGTG
    TTCAATGATA AGAGCCCAGA AGAGGTGTTC ATGGAGATCA GGAGACCACT TGAATGGCAC
    TGCAAACAGA TGGATCATTT TGTTGGGCTA AATTTCAACT CCAACTTCAA CTTTGCACTA
    GTGGGGCACC TCCTGAAAGG GTACAGGCAT CCTTCACCTA CCACTGTAGC AAGAACAGTA
    AGGATTCTAC ATACACTACT AGCTCTGGTT AACAAACACA GGAACTGTGA CAAGTTTGAG
    GTGAACACCC AGAGCGTGGC TTACCTAGCA GCTTTGCTGA CAGTGTCTGA GGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGGAAGTCT CTCTTGCTGA CAGATGTTGC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATACATCAC AGTGACACTA CCTATAGGAC ACTAAAGGAA
    AATCAACCAT GGTCATCACC AAAGGGATCA GACAGACACC TGGCTGCCAG TTACCCGACA
    GTGGGACAGA TAAGCCCTCG AACACGAAAG TCCATGAGTT TGGATATGGG GCAGCCTTCA
    CAAGCGAACA CTAAAAAGCT TCTAGGTACA AGGAAAAGTT TTGACCATTT GATATCGGAC
    ACAAAGGCTC CTAAAAGGCA AGACATGGAA TCTGGAATCA CAACGCCTCC CAAAATGAGG
    AGAGTAGCCG AAAATGATTA TGAAATGGAA ACCCAGAGAA TATCACCGCA GCAGCACCCC
    CATCTCCGGA AAGTTTCTGT TTCTGAGTCA AATGTCCTCC TGGATGAAGA AGTTCTGACT
    GATCCAAAAA TTCAAGCACT GCTGCTTACA GTTCTTGCCA CGTTAGTGAA GTACACTACT
    GATGAATTTG ACCAGCGGAT TCTGTATGAG TATTTAGCAG AAGCCAGTGT TGTCTTCCCA
    AAGGTCTTTC CTGTTGTGCA TAATTTATTG GATTCCAAGA TCAATACCCT CTTGTCGCTG
    TGTCAAGATC CAAATTTGTT GAATCCAATT CATGGGATTG TTCAGAGTGT TGTCTACCAT
    GAGGAGTCTC CACCCCAATA CCAAACTTCT TATCTACAAA GTTTTGGATT TAATGGGTTG
    TGGAGGTTTG CTGGCCCATT TTCCAAGCAA ACACAAATCC CAGACTATGC TGAGCTCATA
    GTGAAGTTTC TTGATGCCTT GATTGACACG TATTTGCCTG GAATTGATGA GGAAACCAGT
    GAAGAATCTC TCCTGACACC CACGTCTCCA TATCCTCCCG CAGTGCAGAG CCAGCTTAGC
    ATTACAGCTA ACCTTAACCT TTCCAATTCC ATGACCTCGC TCGCAACTTC CCAGCACTCC
    CCAGGGATCG ACAAGGAGAA CGTGGAGCTC TCGCCCACCA CTGGCCACAC CAACAGCGGG
    AGGATGCGCC ACGGCTCCGC CAGCCAGGTG CAAAAGCAGC GAAGTGCTGG CAGCTTGAAA
    CGTCACAGCA TTAAGAAGAT TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004946842.1
    CDS237..8783
    Translation

    Target ORF information:

    RefSeq Version XM_004946785.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofibromin 1 (NF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004946785.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGATGCCAAC CAGTCACTGT TTTGCATGGC AGTGGGCCTC TGTTGCTAGT
    CTGACATTTA AAGAGAAAAT GACAAGCCTT AAATTTAAAG AAAAGCCTAC TGACTTGGAA
    ACCAAAAGCT ACAAGTTCCT GCTGTTATCC TTGGTAAAAC TGATCCATGC TGATCCAAAG
    CTTTTGCTGT GTAATCCAAG GAAGCAAGGG CCTGAGACAC AAGGCAGCAC TGCTGAATTA
    ATTACAGGCC TTGTACAACT CGTTCCACAA TCCAGCATGC CAGATATAGC ACAAGAAGCC
    ATGGAGGCCT TGCTAGTTCT TCACCAGTTA GACAGCATTG ACTTATGGAA TCCTGATGCA
    CCTATAGAAA CTTTCTGGGA AATTAGTTCA CAAATGCTTT TTTATATCTG CAAGAAATTA
    ACCAGTCATC AGATGCTCAG CAGTACAGAA ATCCTCAAGT GGCTGCGAGA GATTCTCATC
    TGTAGGAATA AATTTCTTCT AAAAAATAAA CAATCAGACA GAACTTCCTG TCACTTTCTC
    TTCCTTTATG ATGTCTCTGG AGGTGGAGCT AGTCAGATGT CCCTTGACCA CGAAGAGATG
    ATGCGCTGTG CTCCCGGAGC CACCCTCAGA AAAGGAAAAG GGAATTCTTC CATGGAAAGT
    ACAGCTGGAT GCAGTGGAAC ACCCATTTGT CGCCAGGCCC AAACAAAATT GGAAGTTGCC
    TTATACATGT TTTTGTGGAG TCCGGATATT GAGGCAGTCC TTGTTGCAAT GTCCTGTTTC
    CGTCACCTCT GTGAGGAAGC AGATATCAGA TGTGGAGTAG ATGAAGTTTC AGTTCATAAT
    TTTTTGCCAA ACTACAACAC TTTCATGGAG TTTGCATCTG TTAGCAATAT GATGTCAACA
    GGGAGAGCAG CTCTTCAGAA AAGAGTGATG GCTTTATTAA GACGTATTGA ACATCCAACT
    GCAGGGAACA CAGAGGCTTG GGAGGATACA CATGCAAAAT GGGAACAAGC TACAAAACTA
    ATCCTTAACT ATCCAAAGAC CAAAATGGAA GACGGGCAGG TTACTGAAAG TCTTCATAAG
    ACAATTGTTA AGAGACGAAT GTCACATGTT AGTGGGGGAG GCTCCATAGA CTTGTCTGAC
    ACAGATTCTC TTCAGGAATG GATCAACATG ACGGGATTCC TCTGTGCCCT CGGAGGAGTG
    TGCCTGCAGC ATCGTAGCAA TGCAGGATTA GCAACCTACA GTCCACCCAT GGGCCCAGTC
    AATGAGCGTA AGGGCTCCAT GATATCTATG GTATCCACTG AGGGAAATGC AGAGACTCCT
    GTTAGCAAAT TCATAGATCG ATTACTGACT CTGATGGTCT GTAACCATGA GAAAGTGGGA
    CTACAAATAC GGACTAATAT TAAAGATCTG GTGGGTTTGG AGTTGAGTCC AGCACTTTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGATTC TCAAGGACAG
    GTTTTGTTAA CTGAGACCAA CACACAGTTT GTAGAACAAA CCATTGCTAT AATGAAGAAC
    TTGCTTGACA ATCATACAGA AGGGAGCTCA GAACATCTTG GACAAGCTAG TATTGAGACA
    ATGATGCTAA ACCTGGTTAG GTATGTGCGT GTACTTGGAA ATTTGGTACA TGCAATTCAA
    ATAAAAACTA AACTCTGTCA GTTGGTGGAA GTAATGATGG AGCGGAGAGA TGACCTTTCA
    TTTTGCCAAG AAATGAAATT TAGGAACAAA ATGGTGGAAT ATCTGACAGA CTGGGTTATG
    GGAACATCTA ATCAAGCAAC AGATGAAGAT GTGAAGTGCT TGACAAGAGA TTTGGACCAA
    GCAAGTATGG AAGCAGTGGT CTCTCTTCTT GCTGGGCTTC CGTTACAGCC TGAAGAAGGA
    GATGGTGTTG AGTTGATGGA AGCCAAATCT CAGTTATTTC TTAAATATTT CACACTGTTT
    ATGAACCTTC TAAATGACTG TAGTGAAGTT GAAGATGAAA GTGCACAAAC AGGTGGCAGG
    AAACGTGGGA TGTCCCGAAG ACTGGCTTCG CTGCGGCACT GCACTGTTCT TGCTATGTCA
    AATTTACTAA ATGCAAATGT GGACAGTGGT CTTATGCATT CAATAGGCTT GGGCTATCAT
    AAAGACCTCC AGACAAGAGC TACATTTATG GAAGTCCTCA CCAAAATTCT ACAGCAGGGA
    ACAGAATTTG ATACGTTAGC AGAAACGGTG CTGGCAGATC GGTTTGAGAG GCTGGTGGAG
    CTAGTTACAA TGATGGGTGA TCAGGGGGAG CTTCCTATTG CTATGGCTTT AGCAAATGTG
    GTGCCTTGTT CTCAGTGGGA TGAGCTAGCT CGTGTTCTGG TCACACTCTT TGATTCCCGG
    CACTTGCTCT ACCAGCTACT TTGGAATATG TTTTCAAAGG AAGTTGAACT GGCAGATTCC
    ATGCAGACAC TCTTCCGAGG AAACAGCTTA GCTAGTAAAA TAATGACTTT CTGTTTTAAG
    GTATATGGTG CTACTTATTT GCAGAAGCTT TTGGAACCTT TATTAAGAAC AGTGATCACA
    TCCTCTGAAT GGCAGCATGT TAGCTTTGAG GTGGATCCTA CAAGACTGGA GCCTACAGAA
    AGCCTTGAAG AGAACCAGCG GAGTCTCTTG CAAATGACAG AAAAATTTTT TCATGCAATC
    ATTAATTCTT CTTCGGAATT CCCACCACAA CTTCGCAGTG TGTGCCATTG TTTGTACCAG
    GCAACTTGCC ACTCTCTACT GAATAAAGCT ACCGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTTGTTA GTCAGCGATT TCCTCAGAAC AGCATTGGAG CAGTTGGGAG TGCCATGTTC
    CTGCGGTTCA TCAACCCTGC GATCGTCTCC CCGTACGAGG CAGGGATTTT AGATAAAAAG
    CCTCCGCCCA GAATTGAGAG GGGACTGAAA CTGATGTCAA AGATACTTCA GAGTATTGCC
    AACCACGTCC TGTTTACAAA AGAAGAACAT ATGAGGCCTT TTAATGACTT CGTAAAAAGC
    AATTTTGATG CAGCTCGAAG GTTTTTCCTT GATATTGCAT CTGATTGCCC TGCGAGTGAC
    ACTGTCAATC ACAGCCTGTC TTTCATCAGT GATGGCAATG TTCTTGCTTT GCACCGGTTG
    TTGTGGAACA ATCAGGAGAA AATTGGCCAG TACCTTTCCA GTAATAGGGA CCACAAAGCT
    GTTGGAAGGC GACCTTTTGA CAAAATGGCT ACTCTTCTTG CCTATCTGGG TCCACCAGAG
    CACAAACCTG TGGCAGATAC ACATTGGTCC AGTTTAAACC TCACCAGTTC GAAATTTGAA
    GAATTTATGA CAAGGCATCA GGTACATGAA AAAGAAGAGT TCAAAGCATT GAAAACGTTA
    AATATTTTCT ACCAGGCTGG GACATCCAAA GCTGGCAATC CTGTTTTCTA CTACATTGCT
    CGGCGTTTCA AGACTGGTCA GATCAATGGA GATTTATTGA TATACCACGT CCTGCTAACT
    CTGAAGCCAT ACTACGCAAA GCCATATGAA ATTGTAGTGG ACCTCACACA TGCTGGCCCC
    AGTAATCGCT TTAAAACAGA CTTTCTTTCT AAATGGTTTG TAGTTTTTCC AGGCTTTGCT
    TATGAAAATG TCTCAGCAGT TTTTATTTAT AACTGTAATT CTTGGGTCAG AGAATACACC
    AAATACCACG AAAGACTCTT GACAGGTTTG AAAGGAAGCA AAAGGCTTAT TTTCATAGAC
    TCTCCAGGAA AACTGGCGGA GCACATTGAA CATGACCAGC AGAAACTTCC AGCAGCTACC
    TTAGCTTTGG AAGAGGACTT GAAAGTATTT CACAATGCCC TCAAATTGGC TCACAAAGAC
    ACCAAAGTGT CTATTAAAGT TGGATCAACA GCTGTGCAGG TGACATCAGC AGAACGAACA
    AGAGTCCTGG GACAGACAGT GTTTTTAAAT GATATATACT ACGCCTCTGA AATTGAGGAA
    ATATGTCTTG TTGATGAGAA TCAGTTTACA TTGACGATTG CCAACCAGGG AACGCCACTC
    ACCTTCATGC ATCAGGAGTG TGAAGCCATT GTGAGGTCCA TTATTCATAT ACGGACACGG
    TGGGAGCTGT CGCAACCAGA CTCCATCCCA CAGCACACTA AAATTCGCCC TAAGGATGTT
    CCCGGAACAC TACTGAACAT AGCATTGCTC AACCTGGGAA GCTCAGACCC GAGTTTGCGG
    TCTGCTGCCT ATAATCTTCT GTGTGCCTTA ACCTGTACCT TTAATTTAAA GATTGAGGGC
    CAGTTACTGG AAACTTCAGG TCTGTGTATT CCTGCCAACA ACACGCTCTT TATTGTGTCT
    ATTAGTAAGA CTCTTGCAGC TAATGAGCCA CACCTTACCC TAGAGTTCTT GGAAGAGTGT
    ATTTCAGGAT TTAGCAAATC TAGTATTGAA CTGAAACATC TTTGTCTGGA ATATATGACT
    CCCTGGCTGT CCAATCTGGT CCGATTCTGC AAACACAACG ACGATGCGAA GCGCCAGCGA
    GTCACTGCCA TTCTTGATAA GCTTATAACA ATGACAATAA ATGAAAAACA GATGTACCCT
    TCTATTCAGG CTAAAATATG GGGAAGTCTT GGACAGATTA CAGATCTCCT TGACGTAGTG
    CTGGACAGTT TCATCAAAAC TAGTGCCACG GGGGGCTTAG GATCCATTAA AGCTGAGGTT
    ATGGCAGACA CAGCAGTAGC GCTTGCTTCT GGAAATGTAA AATTGGTTTC AAGCAAAGTG
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACTTGTCTGT CTCCAACTCC TACACTGGAA
    CAGCACCTGA TGTGGGATGA TATTGCCATT CTAGCACGTT ACATGCTGAT GCTCTCCTTC
    AACAATTCTC TGGATGTGGC TGCACATCTC CCCTACCTCT TTCATGTGGT TACTTTGTTG
    GTGGCCACTG GACCCCTTTC TCTCAGAGCT TCCACTCATG GTCTTGTCAT CAACATCATT
    CATTCTCTGT GCACTTGCTC ACAGCTTCAT TTTAGTGAGG AGACCAAGCA AGTTTTAAGA
    CTGAGCTTGA CTGAATTTTC TCTGCCTAAA TTTTATTTGC TGTTTGGAAT CAGCAAAGTG
    AAATCAGCTG CAGTCATAGC ATTCCGCTCC AGCTATCGAG ACAGGTCATT TTCACCTGGC
    TCCTACGAGA GGGAGACTTT TGCACTGACC TCCTTGGAAA CTGTTACTGA AGCTTTGCTG
    GAGATCATGG AGGCTTGTAT GAGAGATATT CCAACATGCA AGTGGCTGGA CCAGTGGACC
    GAACTAGCAC AAAAGTTTGC ATTCCAGTAC AATCCATCCC TGCAGCCAAG AGCACTCGTT
    GTCTTTGGCT GTATAAGTAA GCGTGTCTCT CATGGACAAA TAAAACAAAT AATCCGAATT
    CTTAGCAAGG GGCTTGAGAG CTGTTTAAAA GGCCCTGATA ATTACAATAG CCAAGTTCTA
    ATAGAAGCTA CGGTTATAGC ACTCACCAAA CTGCAGCCTC TTCTTAACAA GGACTCCCCC
    ATGCACAAGG CCCTCTTCTG GGTAGCCATG GCAGTACTGC AGTTGGATGA AGTCAACCTA
    TACTCAGCAG GTACAGCACT GCTTGAACAG AATCTGCATA CCTTGGATAG CCTTCGAGTG
    TTCAATGATA AGAGCCCAGA AGAGGTGTTC ATGGAGATCA GGAGACCACT TGAATGGCAC
    TGCAAACAGA TGGATCATTT TGTTGGGCTA AATTTCAACT CCAACTTCAA CTTTGCACTA
    GTGGGGCACC TCCTGAAAGG GTACAGGCAT CCTTCACCTA CCACTGTAGC AAGAACAGTA
    AGGATTCTAC ATACACTACT AGCTCTGGTT AACAAACACA GGAACTGTGA CAAGTTTGAG
    GTGAACACCC AGAGCGTGGC TTACCTAGCA GCTTTGCTGA CAGTGTCTGA GGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGGAAGTCT CTCTTGCTGA CAGATGTTGC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATACATCAC AGTGACACTA CCTATAGGAC ACTAAAGGAA
    AATCAACCAT GGTCATCACC AAAGGGATCA GACAGACACC TGGCTGCCAG TTACCCGACA
    GTGGGACAGA TAAGCCCTCG AACACGAAAG TCCATGAGTT TGGATATGGG GCAGCCTTCA
    CAAGCGAACA CTAAAAAGCT TCTAGGTACA AGGAAAAGTT TTGACCATTT GATATCGGAC
    ACAAAGGCTC CTAAAAGGCA AGACATGGAA TCTGGAATCA CAACGCCTCC CAAAATGAGG
    AGAGTAGCCG AAAATGATTA TGAAATGGAA ACCCAGAGAA TATCACCGCA GCAGCACCCC
    CATCTCCGGA AAGTTTCTGT TTCTGAGTCA AATGTCCTCC TGGATGAAGA AGTTCTGACT
    GATCCAAAAA TTCAAGCACT GCTGCTTACA GTTCTTGCCA CGTTAGTGAA GTACACTACT
    GATGAATTTG ACCAGCGGAT TCTGTATGAG TATTTAGCAG AAGCCAGTGT TGTCTTCCCA
    AAGGTCTTTC CTGTTGTGCA TAATTTATTG GATTCCAAGA TCAATACCCT CTTGTCGCTG
    TGTCAAGATC CAAATTTGTT GAATCCAATT CATGGGATTG TTCAGAGTGT TGTCTACCAT
    GAGGAGTCTC CACCCCAATA CCAAACTTCT TATCTACAAA GTTTTGGATT TAATGGGTTG
    TGGAGGTTTG CTGGCCCATT TTCCAAGCAA ACACAAATCC CAGACTATGC TGAGCTCATA
    GTGAAGTTTC TTGATGCCTT GATTGACACG TATTTGCCTG GAATTGATGA GGAAACCAGT
    GAAGAATCTC TCCTGACACC CACGTCTCCA TATCCTCCCG CAGTGCAGAG CCAGCTTAGC
    ATTACAGCTA ACCTTAACCT TTCCAATTCC ATGACCTCGC TCGCAACTTC CCAGCACTCC
    CCAGGGATCG ACAAGGAGAA CGTGGAGCTC TCGCCCACCA CTGGCCACAC CAACAGCGGG
    AGGATGCGCC ACGGCTCCGC CAGCCAGGTG CAAAAGCAGC GAAGTGCTGG CAGCTTGAAA
    CGTCACAGCA TTAAGAAGAT TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17415
    Clone ID Related Accession (Same CDS sequence) XM_004946785.2 , XM_004946785.3 , XM_025142089.1
    Accession Version XM_025142089.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofibromin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGATGCCAAC CAGTCACTGT TTTGCATGGC AGTGGGCCTC TGTTGCTAGT
    CTGACATTTA AAGAGAAAAT GACAAGCCTT AAATTTAAAG AAAAGCCTAC TGACTTGGAA
    ACCAAAAGCT ACAAGTTCCT GCTGTTATCC TTGGTAAAAC TGATCCATGC TGATCCAAAG
    CTTTTGCTGT GTAATCCAAG GAAGCAAGGG CCTGAGACAC AAGGCAGCAC TGCTGAATTA
    ATTACAGGCC TTGTACAACT CGTTCCACAA TCCAGCATGC CAGATATAGC ACAAGAAGCC
    ATGGAGGCCT TGCTAGTTCT TCACCAGTTA GACAGCATTG ACTTATGGAA TCCTGATGCA
    CCTATAGAAA CTTTCTGGGA AATTAGTTCA CAAATGCTTT TTTATATCTG CAAGAAATTA
    ACCAGTCATC AGATGCTCAG CAGTACAGAA ATCCTCAAGT GGCTGCGAGA GATTCTCATC
    TGTAGGAATA AATTTCTTCT AAAAAATAAA CAATCAGACA GAACTTCCTG TCACTTTCTC
    TTCCTTTATG ATGTCTCTGG AGGTGGAGCT AGTCAGATGT CCCTTGACCA CGAAGAGATG
    ATGCGCTGTG CTCCCGGAGC CACCCTCAGA AAAGGAAAAG GGAATTCTTC CATGGAAAGT
    ACAGCTGGAT GCAGTGGAAC ACCCATTTGT CGCCAGGCCC AAACAAAATT GGAAGTTGCC
    TTATACATGT TTTTGTGGAG TCCGGATATT GAGGCAGTCC TTGTTGCAAT GTCCTGTTTC
    CGTCACCTCT GTGAGGAAGC AGATATCAGA TGTGGAGTAG ATGAAGTTTC AGTTCATAAT
    TTTTTGCCAA ACTACAACAC TTTCATGGAG TTTGCATCTG TTAGCAATAT GATGTCAACA
    GGGAGAGCAG CTCTTCAGAA AAGAGTGATG GCTTTATTAA GACGTATTGA ACATCCAACT
    GCAGGGAACA CAGAGGCTTG GGAGGATACA CATGCAAAAT GGGAACAAGC TACAAAACTA
    ATCCTTAACT ATCCAAAGAC CAAAATGGAA GACGGGCAGG TTACTGAAAG TCTTCATAAG
    ACAATTGTTA AGAGACGAAT GTCACATGTT AGTGGGGGAG GCTCCATAGA CTTGTCTGAC
    ACAGATTCTC TTCAGGAATG GATCAACATG ACGGGATTCC TCTGTGCCCT CGGAGGAGTG
    TGCCTGCAGC ATCGTAGCAA TGCAGGATTA GCAACCTACA GTCCACCCAT GGGCCCAGTC
    AATGAGCGTA AGGGCTCCAT GATATCTATG GTATCCACTG AGGGAAATGC AGAGACTCCT
    GTTAGCAAAT TCATAGATCG ATTACTGACT CTGATGGTCT GTAACCATGA GAAAGTGGGA
    CTACAAATAC GGACTAATAT TAAAGATCTG GTGGGTTTGG AGTTGAGTCC AGCACTTTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGATTC TCAAGGACAG
    GTTTTGTTAA CTGAGACCAA CACACAGTTT GTAGAACAAA CCATTGCTAT AATGAAGAAC
    TTGCTTGACA ATCATACAGA AGGGAGCTCA GAACATCTTG GACAAGCTAG TATTGAGACA
    ATGATGCTAA ACCTGGTTAG GTATGTGCGT GTACTTGGAA ATTTGGTACA TGCAATTCAA
    ATAAAAACTA AACTCTGTCA GTTGGTGGAA GTAATGATGG AGCGGAGAGA TGACCTTTCA
    TTTTGCCAAG AAATGAAATT TAGGAACAAA ATGGTGGAAT ATCTGACAGA CTGGGTTATG
    GGAACATCTA ATCAAGCAAC AGATGAAGAT GTGAAGTGCT TGACAAGAGA TTTGGACCAA
    GCAAGTATGG AAGCAGTGGT CTCTCTTCTT GCTGGGCTTC CGTTACAGCC TGAAGAAGGA
    GATGGTGTTG AGTTGATGGA AGCCAAATCT CAGTTATTTC TTAAATATTT CACACTGTTT
    ATGAACCTTC TAAATGACTG TAGTGAAGTT GAAGATGAAA GTGCACAAAC AGGTGGCAGG
    AAACGTGGGA TGTCCCGAAG ACTGGCTTCG CTGCGGCACT GCACTGTTCT TGCTATGTCA
    AATTTACTAA ATGCAAATGT GGACAGTGGT CTTATGCATT CAATAGGCTT GGGCTATCAT
    AAAGACCTCC AGACAAGAGC TACATTTATG GAAGTCCTCA CCAAAATTCT ACAGCAGGGA
    ACAGAATTTG ATACGTTAGC AGAAACGGTG CTGGCAGATC GGTTTGAGAG GCTGGTGGAG
    CTAGTTACAA TGATGGGTGA TCAGGGGGAG CTTCCTATTG CTATGGCTTT AGCAAATGTG
    GTGCCTTGTT CTCAGTGGGA TGAGCTAGCT CGTGTTCTGG TCACACTCTT TGATTCCCGG
    CACTTGCTCT ACCAGCTACT TTGGAATATG TTTTCAAAGG AAGTTGAACT GGCAGATTCC
    ATGCAGACAC TCTTCCGAGG AAACAGCTTA GCTAGTAAAA TAATGACTTT CTGTTTTAAG
    GTATATGGTG CTACTTATTT GCAGAAGCTT TTGGAACCTT TATTAAGAAC AGTGATCACA
    TCCTCTGAAT GGCAGCATGT TAGCTTTGAG GTGGATCCTA CAAGACTGGA GCCTACAGAA
    AGCCTTGAAG AGAACCAGCG GAGTCTCTTG CAAATGACAG AAAAATTTTT TCATGCAATC
    ATTAATTCTT CTTCGGAATT CCCACCACAA CTTCGCAGTG TGTGCCATTG TTTGTACCAG
    GCAACTTGCC ACTCTCTACT GAATAAAGCT ACCGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTTGTTA GTCAGCGATT TCCTCAGAAC AGCATTGGAG CAGTTGGGAG TGCCATGTTC
    CTGCGGTTCA TCAACCCTGC GATCGTCTCC CCGTACGAGG CAGGGATTTT AGATAAAAAG
    CCTCCGCCCA GAATTGAGAG GGGACTGAAA CTGATGTCAA AGATACTTCA GAGTATTGCC
    AACCACGTCC TGTTTACAAA AGAAGAACAT ATGAGGCCTT TTAATGACTT CGTAAAAAGC
    AATTTTGATG CAGCTCGAAG GTTTTTCCTT GATATTGCAT CTGATTGCCC TGCGAGTGAC
    ACTGTCAATC ACAGCCTGTC TTTCATCAGT GATGGCAATG TTCTTGCTTT GCACCGGTTG
    TTGTGGAACA ATCAGGAGAA AATTGGCCAG TACCTTTCCA GTAATAGGGA CCACAAAGCT
    GTTGGAAGGC GACCTTTTGA CAAAATGGCT ACTCTTCTTG CCTATCTGGG TCCACCAGAG
    CACAAACCTG TGGCAGATAC ACATTGGTCC AGTTTAAACC TCACCAGTTC GAAATTTGAA
    GAATTTATGA CAAGGCATCA GGTACATGAA AAAGAAGAGT TCAAAGCATT GAAAACGTTA
    AATATTTTCT ACCAGGCTGG GACATCCAAA GCTGGCAATC CTGTTTTCTA CTACATTGCT
    CGGCGTTTCA AGACTGGTCA GATCAATGGA GATTTATTGA TATACCACGT CCTGCTAACT
    CTGAAGCCAT ACTACGCAAA GCCATATGAA ATTGTAGTGG ACCTCACACA TGCTGGCCCC
    AGTAATCGCT TTAAAACAGA CTTTCTTTCT AAATGGTTTG TAGTTTTTCC AGGCTTTGCT
    TATGAAAATG TCTCAGCAGT TTTTATTTAT AACTGTAATT CTTGGGTCAG AGAATACACC
    AAATACCACG AAAGACTCTT GACAGGTTTG AAAGGAAGCA AAAGGCTTAT TTTCATAGAC
    TCTCCAGGAA AACTGGCGGA GCACATTGAA CATGACCAGC AGAAACTTCC AGCAGCTACC
    TTAGCTTTGG AAGAGGACTT GAAAGTATTT CACAATGCCC TCAAATTGGC TCACAAAGAC
    ACCAAAGTGT CTATTAAAGT TGGATCAACA GCTGTGCAGG TGACATCAGC AGAACGAACA
    AGAGTCCTGG GACAGACAGT GTTTTTAAAT GATATATACT ACGCCTCTGA AATTGAGGAA
    ATATGTCTTG TTGATGAGAA TCAGTTTACA TTGACGATTG CCAACCAGGG AACGCCACTC
    ACCTTCATGC ATCAGGAGTG TGAAGCCATT GTGAGGTCCA TTATTCATAT ACGGACACGG
    TGGGAGCTGT CGCAACCAGA CTCCATCCCA CAGCACACTA AAATTCGCCC TAAGGATGTT
    CCCGGAACAC TACTGAACAT AGCATTGCTC AACCTGGGAA GCTCAGACCC GAGTTTGCGG
    TCTGCTGCCT ATAATCTTCT GTGTGCCTTA ACCTGTACCT TTAATTTAAA GATTGAGGGC
    CAGTTACTGG AAACTTCAGG TCTGTGTATT CCTGCCAACA ACACGCTCTT TATTGTGTCT
    ATTAGTAAGA CTCTTGCAGC TAATGAGCCA CACCTTACCC TAGAGTTCTT GGAAGAGTGT
    ATTTCAGGAT TTAGCAAATC TAGTATTGAA CTGAAACATC TTTGTCTGGA ATATATGACT
    CCCTGGCTGT CCAATCTGGT CCGATTCTGC AAACACAACG ACGATGCGAA GCGCCAGCGA
    GTCACTGCCA TTCTTGATAA GCTTATAACA ATGACAATAA ATGAAAAACA GATGTACCCT
    TCTATTCAGG CTAAAATATG GGGAAGTCTT GGACAGATTA CAGATCTCCT TGACGTAGTG
    CTGGACAGTT TCATCAAAAC TAGTGCCACG GGGGGCTTAG GATCCATTAA AGCTGAGGTT
    ATGGCAGACA CAGCAGTAGC GCTTGCTTCT GGAAATGTAA AATTGGTTTC AAGCAAAGTG
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACTTGTCTGT CTCCAACTCC TACACTGGAA
    CAGCACCTGA TGTGGGATGA TATTGCCATT CTAGCACGTT ACATGCTGAT GCTCTCCTTC
    AACAATTCTC TGGATGTGGC TGCACATCTC CCCTACCTCT TTCATGTGGT TACTTTGTTG
    GTGGCCACTG GACCCCTTTC TCTCAGAGCT TCCACTCATG GTCTTGTCAT CAACATCATT
    CATTCTCTGT GCACTTGCTC ACAGCTTCAT TTTAGTGAGG AGACCAAGCA AGTTTTAAGA
    CTGAGCTTGA CTGAATTTTC TCTGCCTAAA TTTTATTTGC TGTTTGGAAT CAGCAAAGTG
    AAATCAGCTG CAGTCATAGC ATTCCGCTCC AGCTATCGAG ACAGGTCATT TTCACCTGGC
    TCCTACGAGA GGGAGACTTT TGCACTGACC TCCTTGGAAA CTGTTACTGA AGCTTTGCTG
    GAGATCATGG AGGCTTGTAT GAGAGATATT CCAACATGCA AGTGGCTGGA CCAGTGGACC
    GAACTAGCAC AAAAGTTTGC ATTCCAGTAC AATCCATCCC TGCAGCCAAG AGCACTCGTT
    GTCTTTGGCT GTATAAGTAA GCGTGTCTCT CATGGACAAA TAAAACAAAT AATCCGAATT
    CTTAGCAAGG GGCTTGAGAG CTGTTTAAAA GGCCCTGATA ATTACAATAG CCAAGTTCTA
    ATAGAAGCTA CGGTTATAGC ACTCACCAAA CTGCAGCCTC TTCTTAACAA GGACTCCCCC
    ATGCACAAGG CCCTCTTCTG GGTAGCCATG GCAGTACTGC AGTTGGATGA AGTCAACCTA
    TACTCAGCAG GTACAGCACT GCTTGAACAG AATCTGCATA CCTTGGATAG CCTTCGAGTG
    TTCAATGATA AGAGCCCAGA AGAGGTGTTC ATGGAGATCA GGAGACCACT TGAATGGCAC
    TGCAAACAGA TGGATCATTT TGTTGGGCTA AATTTCAACT CCAACTTCAA CTTTGCACTA
    GTGGGGCACC TCCTGAAAGG GTACAGGCAT CCTTCACCTA CCACTGTAGC AAGAACAGTA
    AGGATTCTAC ATACACTACT AGCTCTGGTT AACAAACACA GGAACTGTGA CAAGTTTGAG
    GTGAACACCC AGAGCGTGGC TTACCTAGCA GCTTTGCTGA CAGTGTCTGA GGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGGAAGTCT CTCTTGCTGA CAGATGTTGC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATACATCAC AGTGACACTA CCTATAGGAC ACTAAAGGAA
    AATCAACCAT GGTCATCACC AAAGGGATCA GACAGACACC TGGCTGCCAG TTACCCGACA
    GTGGGACAGA TAAGCCCTCG AACACGAAAG TCCATGAGTT TGGATATGGG GCAGCCTTCA
    CAAGCGAACA CTAAAAAGCT TCTAGGTACA AGGAAAAGTT TTGACCATTT GATATCGGAC
    ACAAAGGCTC CTAAAAGGCA AGACATGGAA TCTGGAATCA CAACGCCTCC CAAAATGAGG
    AGAGTAGCCG AAAATGATTA TGAAATGGAA ACCCAGAGAA TATCACCGCA GCAGCACCCC
    CATCTCCGGA AAGTTTCTGT TTCTGAGTCA AATGTCCTCC TGGATGAAGA AGTTCTGACT
    GATCCAAAAA TTCAAGCACT GCTGCTTACA GTTCTTGCCA CGTTAGTGAA GTACACTACT
    GATGAATTTG ACCAGCGGAT TCTGTATGAG TATTTAGCAG AAGCCAGTGT TGTCTTCCCA
    AAGGTCTTTC CTGTTGTGCA TAATTTATTG GATTCCAAGA TCAATACCCT CTTGTCGCTG
    TGTCAAGATC CAAATTTGTT GAATCCAATT CATGGGATTG TTCAGAGTGT TGTCTACCAT
    GAGGAGTCTC CACCCCAATA CCAAACTTCT TATCTACAAA GTTTTGGATT TAATGGGTTG
    TGGAGGTTTG CTGGCCCATT TTCCAAGCAA ACACAAATCC CAGACTATGC TGAGCTCATA
    GTGAAGTTTC TTGATGCCTT GATTGACACG TATTTGCCTG GAATTGATGA GGAAACCAGT
    GAAGAATCTC TCCTGACACC CACGTCTCCA TATCCTCCCG CAGTGCAGAG CCAGCTTAGC
    ATTACAGCTA ACCTTAACCT TTCCAATTCC ATGACCTCGC TCGCAACTTC CCAGCACTCC
    CCAGGGATCG ACAAGGAGAA CGTGGAGCTC TCGCCCACCA CTGGCCACAC CAACAGCGGG
    AGGATGCGCC ACGGCTCCGC CAGCCAGGTG CAAAAGCAGC GAAGTGCTGG CAGCTTGAAA
    CGTCACAGCA TTAAGAAGAT TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024997857.1
    CDS237..8783
    Translation

    Target ORF information:

    RefSeq Version XM_025142089.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofibromin 1 (NF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025142089.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGATGCCAAC CAGTCACTGT TTTGCATGGC AGTGGGCCTC TGTTGCTAGT
    CTGACATTTA AAGAGAAAAT GACAAGCCTT AAATTTAAAG AAAAGCCTAC TGACTTGGAA
    ACCAAAAGCT ACAAGTTCCT GCTGTTATCC TTGGTAAAAC TGATCCATGC TGATCCAAAG
    CTTTTGCTGT GTAATCCAAG GAAGCAAGGG CCTGAGACAC AAGGCAGCAC TGCTGAATTA
    ATTACAGGCC TTGTACAACT CGTTCCACAA TCCAGCATGC CAGATATAGC ACAAGAAGCC
    ATGGAGGCCT TGCTAGTTCT TCACCAGTTA GACAGCATTG ACTTATGGAA TCCTGATGCA
    CCTATAGAAA CTTTCTGGGA AATTAGTTCA CAAATGCTTT TTTATATCTG CAAGAAATTA
    ACCAGTCATC AGATGCTCAG CAGTACAGAA ATCCTCAAGT GGCTGCGAGA GATTCTCATC
    TGTAGGAATA AATTTCTTCT AAAAAATAAA CAATCAGACA GAACTTCCTG TCACTTTCTC
    TTCCTTTATG ATGTCTCTGG AGGTGGAGCT AGTCAGATGT CCCTTGACCA CGAAGAGATG
    ATGCGCTGTG CTCCCGGAGC CACCCTCAGA AAAGGAAAAG GGAATTCTTC CATGGAAAGT
    ACAGCTGGAT GCAGTGGAAC ACCCATTTGT CGCCAGGCCC AAACAAAATT GGAAGTTGCC
    TTATACATGT TTTTGTGGAG TCCGGATATT GAGGCAGTCC TTGTTGCAAT GTCCTGTTTC
    CGTCACCTCT GTGAGGAAGC AGATATCAGA TGTGGAGTAG ATGAAGTTTC AGTTCATAAT
    TTTTTGCCAA ACTACAACAC TTTCATGGAG TTTGCATCTG TTAGCAATAT GATGTCAACA
    GGGAGAGCAG CTCTTCAGAA AAGAGTGATG GCTTTATTAA GACGTATTGA ACATCCAACT
    GCAGGGAACA CAGAGGCTTG GGAGGATACA CATGCAAAAT GGGAACAAGC TACAAAACTA
    ATCCTTAACT ATCCAAAGAC CAAAATGGAA GACGGGCAGG TTACTGAAAG TCTTCATAAG
    ACAATTGTTA AGAGACGAAT GTCACATGTT AGTGGGGGAG GCTCCATAGA CTTGTCTGAC
    ACAGATTCTC TTCAGGAATG GATCAACATG ACGGGATTCC TCTGTGCCCT CGGAGGAGTG
    TGCCTGCAGC ATCGTAGCAA TGCAGGATTA GCAACCTACA GTCCACCCAT GGGCCCAGTC
    AATGAGCGTA AGGGCTCCAT GATATCTATG GTATCCACTG AGGGAAATGC AGAGACTCCT
    GTTAGCAAAT TCATAGATCG ATTACTGACT CTGATGGTCT GTAACCATGA GAAAGTGGGA
    CTACAAATAC GGACTAATAT TAAAGATCTG GTGGGTTTGG AGTTGAGTCC AGCACTTTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGATTC TCAAGGACAG
    GTTTTGTTAA CTGAGACCAA CACACAGTTT GTAGAACAAA CCATTGCTAT AATGAAGAAC
    TTGCTTGACA ATCATACAGA AGGGAGCTCA GAACATCTTG GACAAGCTAG TATTGAGACA
    ATGATGCTAA ACCTGGTTAG GTATGTGCGT GTACTTGGAA ATTTGGTACA TGCAATTCAA
    ATAAAAACTA AACTCTGTCA GTTGGTGGAA GTAATGATGG AGCGGAGAGA TGACCTTTCA
    TTTTGCCAAG AAATGAAATT TAGGAACAAA ATGGTGGAAT ATCTGACAGA CTGGGTTATG
    GGAACATCTA ATCAAGCAAC AGATGAAGAT GTGAAGTGCT TGACAAGAGA TTTGGACCAA
    GCAAGTATGG AAGCAGTGGT CTCTCTTCTT GCTGGGCTTC CGTTACAGCC TGAAGAAGGA
    GATGGTGTTG AGTTGATGGA AGCCAAATCT CAGTTATTTC TTAAATATTT CACACTGTTT
    ATGAACCTTC TAAATGACTG TAGTGAAGTT GAAGATGAAA GTGCACAAAC AGGTGGCAGG
    AAACGTGGGA TGTCCCGAAG ACTGGCTTCG CTGCGGCACT GCACTGTTCT TGCTATGTCA
    AATTTACTAA ATGCAAATGT GGACAGTGGT CTTATGCATT CAATAGGCTT GGGCTATCAT
    AAAGACCTCC AGACAAGAGC TACATTTATG GAAGTCCTCA CCAAAATTCT ACAGCAGGGA
    ACAGAATTTG ATACGTTAGC AGAAACGGTG CTGGCAGATC GGTTTGAGAG GCTGGTGGAG
    CTAGTTACAA TGATGGGTGA TCAGGGGGAG CTTCCTATTG CTATGGCTTT AGCAAATGTG
    GTGCCTTGTT CTCAGTGGGA TGAGCTAGCT CGTGTTCTGG TCACACTCTT TGATTCCCGG
    CACTTGCTCT ACCAGCTACT TTGGAATATG TTTTCAAAGG AAGTTGAACT GGCAGATTCC
    ATGCAGACAC TCTTCCGAGG AAACAGCTTA GCTAGTAAAA TAATGACTTT CTGTTTTAAG
    GTATATGGTG CTACTTATTT GCAGAAGCTT TTGGAACCTT TATTAAGAAC AGTGATCACA
    TCCTCTGAAT GGCAGCATGT TAGCTTTGAG GTGGATCCTA CAAGACTGGA GCCTACAGAA
    AGCCTTGAAG AGAACCAGCG GAGTCTCTTG CAAATGACAG AAAAATTTTT TCATGCAATC
    ATTAATTCTT CTTCGGAATT CCCACCACAA CTTCGCAGTG TGTGCCATTG TTTGTACCAG
    GCAACTTGCC ACTCTCTACT GAATAAAGCT ACCGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTTGTTA GTCAGCGATT TCCTCAGAAC AGCATTGGAG CAGTTGGGAG TGCCATGTTC
    CTGCGGTTCA TCAACCCTGC GATCGTCTCC CCGTACGAGG CAGGGATTTT AGATAAAAAG
    CCTCCGCCCA GAATTGAGAG GGGACTGAAA CTGATGTCAA AGATACTTCA GAGTATTGCC
    AACCACGTCC TGTTTACAAA AGAAGAACAT ATGAGGCCTT TTAATGACTT CGTAAAAAGC
    AATTTTGATG CAGCTCGAAG GTTTTTCCTT GATATTGCAT CTGATTGCCC TGCGAGTGAC
    ACTGTCAATC ACAGCCTGTC TTTCATCAGT GATGGCAATG TTCTTGCTTT GCACCGGTTG
    TTGTGGAACA ATCAGGAGAA AATTGGCCAG TACCTTTCCA GTAATAGGGA CCACAAAGCT
    GTTGGAAGGC GACCTTTTGA CAAAATGGCT ACTCTTCTTG CCTATCTGGG TCCACCAGAG
    CACAAACCTG TGGCAGATAC ACATTGGTCC AGTTTAAACC TCACCAGTTC GAAATTTGAA
    GAATTTATGA CAAGGCATCA GGTACATGAA AAAGAAGAGT TCAAAGCATT GAAAACGTTA
    AATATTTTCT ACCAGGCTGG GACATCCAAA GCTGGCAATC CTGTTTTCTA CTACATTGCT
    CGGCGTTTCA AGACTGGTCA GATCAATGGA GATTTATTGA TATACCACGT CCTGCTAACT
    CTGAAGCCAT ACTACGCAAA GCCATATGAA ATTGTAGTGG ACCTCACACA TGCTGGCCCC
    AGTAATCGCT TTAAAACAGA CTTTCTTTCT AAATGGTTTG TAGTTTTTCC AGGCTTTGCT
    TATGAAAATG TCTCAGCAGT TTTTATTTAT AACTGTAATT CTTGGGTCAG AGAATACACC
    AAATACCACG AAAGACTCTT GACAGGTTTG AAAGGAAGCA AAAGGCTTAT TTTCATAGAC
    TCTCCAGGAA AACTGGCGGA GCACATTGAA CATGACCAGC AGAAACTTCC AGCAGCTACC
    TTAGCTTTGG AAGAGGACTT GAAAGTATTT CACAATGCCC TCAAATTGGC TCACAAAGAC
    ACCAAAGTGT CTATTAAAGT TGGATCAACA GCTGTGCAGG TGACATCAGC AGAACGAACA
    AGAGTCCTGG GACAGACAGT GTTTTTAAAT GATATATACT ACGCCTCTGA AATTGAGGAA
    ATATGTCTTG TTGATGAGAA TCAGTTTACA TTGACGATTG CCAACCAGGG AACGCCACTC
    ACCTTCATGC ATCAGGAGTG TGAAGCCATT GTGAGGTCCA TTATTCATAT ACGGACACGG
    TGGGAGCTGT CGCAACCAGA CTCCATCCCA CAGCACACTA AAATTCGCCC TAAGGATGTT
    CCCGGAACAC TACTGAACAT AGCATTGCTC AACCTGGGAA GCTCAGACCC GAGTTTGCGG
    TCTGCTGCCT ATAATCTTCT GTGTGCCTTA ACCTGTACCT TTAATTTAAA GATTGAGGGC
    CAGTTACTGG AAACTTCAGG TCTGTGTATT CCTGCCAACA ACACGCTCTT TATTGTGTCT
    ATTAGTAAGA CTCTTGCAGC TAATGAGCCA CACCTTACCC TAGAGTTCTT GGAAGAGTGT
    ATTTCAGGAT TTAGCAAATC TAGTATTGAA CTGAAACATC TTTGTCTGGA ATATATGACT
    CCCTGGCTGT CCAATCTGGT CCGATTCTGC AAACACAACG ACGATGCGAA GCGCCAGCGA
    GTCACTGCCA TTCTTGATAA GCTTATAACA ATGACAATAA ATGAAAAACA GATGTACCCT
    TCTATTCAGG CTAAAATATG GGGAAGTCTT GGACAGATTA CAGATCTCCT TGACGTAGTG
    CTGGACAGTT TCATCAAAAC TAGTGCCACG GGGGGCTTAG GATCCATTAA AGCTGAGGTT
    ATGGCAGACA CAGCAGTAGC GCTTGCTTCT GGAAATGTAA AATTGGTTTC AAGCAAAGTG
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACTTGTCTGT CTCCAACTCC TACACTGGAA
    CAGCACCTGA TGTGGGATGA TATTGCCATT CTAGCACGTT ACATGCTGAT GCTCTCCTTC
    AACAATTCTC TGGATGTGGC TGCACATCTC CCCTACCTCT TTCATGTGGT TACTTTGTTG
    GTGGCCACTG GACCCCTTTC TCTCAGAGCT TCCACTCATG GTCTTGTCAT CAACATCATT
    CATTCTCTGT GCACTTGCTC ACAGCTTCAT TTTAGTGAGG AGACCAAGCA AGTTTTAAGA
    CTGAGCTTGA CTGAATTTTC TCTGCCTAAA TTTTATTTGC TGTTTGGAAT CAGCAAAGTG
    AAATCAGCTG CAGTCATAGC ATTCCGCTCC AGCTATCGAG ACAGGTCATT TTCACCTGGC
    TCCTACGAGA GGGAGACTTT TGCACTGACC TCCTTGGAAA CTGTTACTGA AGCTTTGCTG
    GAGATCATGG AGGCTTGTAT GAGAGATATT CCAACATGCA AGTGGCTGGA CCAGTGGACC
    GAACTAGCAC AAAAGTTTGC ATTCCAGTAC AATCCATCCC TGCAGCCAAG AGCACTCGTT
    GTCTTTGGCT GTATAAGTAA GCGTGTCTCT CATGGACAAA TAAAACAAAT AATCCGAATT
    CTTAGCAAGG GGCTTGAGAG CTGTTTAAAA GGCCCTGATA ATTACAATAG CCAAGTTCTA
    ATAGAAGCTA CGGTTATAGC ACTCACCAAA CTGCAGCCTC TTCTTAACAA GGACTCCCCC
    ATGCACAAGG CCCTCTTCTG GGTAGCCATG GCAGTACTGC AGTTGGATGA AGTCAACCTA
    TACTCAGCAG GTACAGCACT GCTTGAACAG AATCTGCATA CCTTGGATAG CCTTCGAGTG
    TTCAATGATA AGAGCCCAGA AGAGGTGTTC ATGGAGATCA GGAGACCACT TGAATGGCAC
    TGCAAACAGA TGGATCATTT TGTTGGGCTA AATTTCAACT CCAACTTCAA CTTTGCACTA
    GTGGGGCACC TCCTGAAAGG GTACAGGCAT CCTTCACCTA CCACTGTAGC AAGAACAGTA
    AGGATTCTAC ATACACTACT AGCTCTGGTT AACAAACACA GGAACTGTGA CAAGTTTGAG
    GTGAACACCC AGAGCGTGGC TTACCTAGCA GCTTTGCTGA CAGTGTCTGA GGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGGAAGTCT CTCTTGCTGA CAGATGTTGC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATACATCAC AGTGACACTA CCTATAGGAC ACTAAAGGAA
    AATCAACCAT GGTCATCACC AAAGGGATCA GACAGACACC TGGCTGCCAG TTACCCGACA
    GTGGGACAGA TAAGCCCTCG AACACGAAAG TCCATGAGTT TGGATATGGG GCAGCCTTCA
    CAAGCGAACA CTAAAAAGCT TCTAGGTACA AGGAAAAGTT TTGACCATTT GATATCGGAC
    ACAAAGGCTC CTAAAAGGCA AGACATGGAA TCTGGAATCA CAACGCCTCC CAAAATGAGG
    AGAGTAGCCG AAAATGATTA TGAAATGGAA ACCCAGAGAA TATCACCGCA GCAGCACCCC
    CATCTCCGGA AAGTTTCTGT TTCTGAGTCA AATGTCCTCC TGGATGAAGA AGTTCTGACT
    GATCCAAAAA TTCAAGCACT GCTGCTTACA GTTCTTGCCA CGTTAGTGAA GTACACTACT
    GATGAATTTG ACCAGCGGAT TCTGTATGAG TATTTAGCAG AAGCCAGTGT TGTCTTCCCA
    AAGGTCTTTC CTGTTGTGCA TAATTTATTG GATTCCAAGA TCAATACCCT CTTGTCGCTG
    TGTCAAGATC CAAATTTGTT GAATCCAATT CATGGGATTG TTCAGAGTGT TGTCTACCAT
    GAGGAGTCTC CACCCCAATA CCAAACTTCT TATCTACAAA GTTTTGGATT TAATGGGTTG
    TGGAGGTTTG CTGGCCCATT TTCCAAGCAA ACACAAATCC CAGACTATGC TGAGCTCATA
    GTGAAGTTTC TTGATGCCTT GATTGACACG TATTTGCCTG GAATTGATGA GGAAACCAGT
    GAAGAATCTC TCCTGACACC CACGTCTCCA TATCCTCCCG CAGTGCAGAG CCAGCTTAGC
    ATTACAGCTA ACCTTAACCT TTCCAATTCC ATGACCTCGC TCGCAACTTC CCAGCACTCC
    CCAGGGATCG ACAAGGAGAA CGTGGAGCTC TCGCCCACCA CTGGCCACAC CAACAGCGGG
    AGGATGCGCC ACGGCTCCGC CAGCCAGGTG CAAAAGCAGC GAAGTGCTGG CAGCTTGAAA
    CGTCACAGCA TTAAGAAGAT TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17416
    Clone ID Related Accession (Same CDS sequence) XM_415914.5 , XM_415914.6
    Accession Version XM_415914.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofibromin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455988.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_415914.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
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    481
    541
    601
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    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGAGTCTGAC ATTTAAAGAG AAAATGACAA GCCTTAAATT TAAAGAAAAG
    CCTACTGACT TGGAAACCAA AAGCTACAAG TTCCTGCTGT TATCCTTGGT AAAACTGATC
    CATGCTGATC CAAAGCTTTT GCTGTGTAAT CCAAGGAAGC AAGGGCCTGA GACACAAGGC
    AGCACTGCTG AATTAATTAC AGGCCTTGTA CAACTCGTTC CACAATCCAG CATGCCAGAT
    ATAGCACAAG AAGCCATGGA GGCCTTGCTA GTTCTTCACC AGTTAGACAG CATTGACTTA
    TGGAATCCTG ATGCACCTAT AGAAACTTTC TGGGAAATTA GTTCACAAAT GCTTTTTTAT
    ATCTGCAAGA AATTAACCAG TCATCAGATG CTCAGCAGTA CAGAAATCCT CAAGTGGCTG
    CGAGAGATTC TCATCTGTAG GAATAAATTT CTTCTAAAAA ATAAACAATC AGACAGAACT
    TCCTGTCACT TTCTCTTCCT TTATGATGTC TCTGGAGGTG GAGCTAGTCA GATGTCCCTT
    GACCACGAAG AGATGATGCG CTGTGCTCCC GGAGCCACCC TCAGAAAAGG AAAAGGGAAT
    TCTTCCATGG AAAGTACAGC TGGATGCAGT GGAACACCCA TTTGTCGCCA GGCCCAAACA
    AAATTGGAAG TTGCCTTATA CATGTTTTTG TGGAGTCCGG ATATTGAGGC AGTCCTTGTT
    GCAATGTCCT GTTTCCGTCA CCTCTGTGAG GAAGCAGATA TCAGATGTGG AGTAGATGAA
    GTTTCAGTTC ATAATTTTTT GCCAAACTAC AACACTTTCA TGGAGTTTGC ATCTGTTAGC
    AATATGATGT CAACAGGGAG AGCAGCTCTT CAGAAAAGAG TGATGGCTTT ATTAAGACGT
    ATTGAACATC CAACTGCAGG GAACACAGAG GCTTGGGAGG ATACACATGC AAAATGGGAA
    CAAGCTACAA AACTAATCCT TAACTATCCA AAGACCAAAA TGGAAGACGG GCAGGTTACT
    GAAAGTCTTC ATAAGACAAT TGTTAAGAGA CGAATGTCAC ATGTTAGTGG GGGAGGCTCC
    ATAGACTTGT CTGACACAGA TTCTCTTCAG GAATGGATCA ACATGACGGG ATTCCTCTGT
    GCCCTCGGAG GAGTGTGCCT GCAGCATCGT AGCAATGCAG GATTAGCAAC CTACAGTCCA
    CCCATGGGCC CAGTCAATGA GCGTAAGGGC TCCATGATAT CTATGGTATC CACTGAGGGA
    AATGCAGAGA CTCCTGTTAG CAAATTCATA GATCGATTAC TGACTCTGAT GGTCTGTAAC
    CATGAGAAAG TGGGACTACA AATACGGACT AATATTAAAG ATCTGGTGGG TTTGGAGTTG
    AGTCCAGCAC TTTATCCAAT GCTATTTAAC AAATTGAAGA ATACCATCAG CAAGTTTTTT
    GATTCTCAAG GACAGGTTTT GTTAACTGAG ACCAACACAC AGTTTGTAGA ACAAACCATT
    GCTATAATGA AGAACTTGCT TGACAATCAT ACAGAAGGGA GCTCAGAACA TCTTGGACAA
    GCTAGTATTG AGACAATGAT GCTAAACCTG GTTAGGTATG TGCGTGTACT TGGAAATTTG
    GTACATGCAA TTCAAATAAA AACTAAACTC TGTCAGTTGG TGGAAGTAAT GATGGAGCGG
    AGAGATGACC TTTCATTTTG CCAAGAAATG AAATTTAGGA ACAAAATGGT GGAATATCTG
    ACAGACTGGG TTATGGGAAC ATCTAATCAA GCAACAGATG AAGATGTGAA GTGCTTGACA
    AGAGATTTGG ACCAAGCAAG TATGGAAGCA GTGGTCTCTC TTCTTGCTGG GCTTCCGTTA
    CAGCCTGAAG AAGGAGATGG TGTTGAGTTG ATGGAAGCCA AATCTCAGTT ATTTCTTAAA
    TATTTCACAC TGTTTATGAA CCTTCTAAAT GACTGTAGTG AAGTTGAAGA TGAAAGTGCA
    CAAACAGGTG GCAGGAAACG TGGGATGTCC CGAAGACTGG CTTCGCTGCG GCACTGCACT
    GTTCTTGCTA TGTCAAATTT ACTAAATGCA AATGTGGACA GTGGTCTTAT GCATTCAATA
    GGCTTGGGCT ATCATAAAGA CCTCCAGACA AGAGCTACAT TTATGGAAGT CCTCACCAAA
    ATTCTACAGC AGGGAACAGA ATTTGATACG TTAGCAGAAA CGGTGCTGGC AGATCGGTTT
    GAGAGGCTGG TGGAGCTAGT TACAATGATG GGTGATCAGG GGGAGCTTCC TATTGCTATG
    GCTTTAGCAA ATGTGGTGCC TTGTTCTCAG TGGGATGAGC TAGCTCGTGT TCTGGTCACA
    CTCTTTGATT CCCGGCACTT GCTCTACCAG CTACTTTGGA ATATGTTTTC AAAGGAAGTT
    GAACTGGCAG ATTCCATGCA GACACTCTTC CGAGGAAACA GCTTAGCTAG TAAAATAATG
    ACTTTCTGTT TTAAGGTATA TGGTGCTACT TATTTGCAGA AGCTTTTGGA ACCTTTATTA
    AGAACAGTGA TCACATCCTC TGAATGGCAG CATGTTAGCT TTGAGGTGGA TCCTACAAGA
    CTGGAGCCTA CAGAAAGCCT TGAAGAGAAC CAGCGGAGTC TCTTGCAAAT GACAGAAAAA
    TTTTTTCATG CAATCATTAA TTCTTCTTCG GAATTCCCAC CACAACTTCG CAGTGTGTGC
    CATTGTTTGT ACCAGGCAAC TTGCCACTCT CTACTGAATA AAGCTACCGT AAAAGAAAAA
    AAGGAAAACA AAAAATCAGT TGTTAGTCAG CGATTTCCTC AGAACAGCAT TGGAGCAGTT
    GGGAGTGCCA TGTTCCTGCG GTTCATCAAC CCTGCGATCG TCTCCCCGTA CGAGGCAGGG
    ATTTTAGATA AAAAGCCTCC GCCCAGAATT GAGAGGGGAC TGAAACTGAT GTCAAAGATA
    CTTCAGAGTA TTGCCAACCA CGTCCTGTTT ACAAAAGAAG AACATATGAG GCCTTTTAAT
    GACTTCGTAA AAAGCAATTT TGATGCAGCT CGAAGGTTTT TCCTTGATAT TGCATCTGAT
    TGCCCTGCGA GTGACACTGT CAATCACAGC CTGTCTTTCA TCAGTGATGG CAATGTTCTT
    GCTTTGCACC GGTTGTTGTG GAACAATCAG GAGAAAATTG GCCAGTACCT TTCCAGTAAT
    AGGGACCACA AAGCTGTTGG AAGGCGACCT TTTGACAAAA TGGCTACTCT TCTTGCCTAT
    CTGGGTCCAC CAGAGCACAA ACCTGTGGCA GATACACATT GGTCCAGTTT AAACCTCACC
    AGTTCGAAAT TTGAAGAATT TATGACAAGG CATCAGGTAC ATGAAAAAGA AGAGTTCAAA
    GCATTGAAAA CGTTAAATAT TTTCTACCAG GCTGGGACAT CCAAAGCTGG CAATCCTGTT
    TTCTACTACA TTGCTCGGCG TTTCAAGACT GGTCAGATCA ATGGAGATTT ATTGATATAC
    CACGTCCTGC TAACTCTGAA GCCATACTAC GCAAAGCCAT ATGAAATTGT AGTGGACCTC
    ACACATGCTG GCCCCAGTAA TCGCTTTAAA ACAGACTTTC TTTCTAAATG GTTTGTAGTT
    TTTCCAGGCT TTGCTTATGA AAATGTCTCA GCAGTTTTTA TTTATAACTG TAATTCTTGG
    GTCAGAGAAT ACACCAAATA CCACGAAAGA CTCTTGACAG GTTTGAAAGG AAGCAAAAGG
    CTTATTTTCA TAGACTCTCC AGGAAAACTG GCGGAGCACA TTGAACATGA CCAGCAGAAA
    CTTCCAGCAG CTACCTTAGC TTTGGAAGAG GACTTGAAAG TATTTCACAA TGCCCTCAAA
    TTGGCTCACA AAGACACCAA AGTGTCTATT AAAGTTGGAT CAACAGCTGT GCAGGTGACA
    TCAGCAGAAC GAACAAGAGT CCTGGGACAG ACAGTGTTTT TAAATGATAT ATACTACGCC
    TCTGAAATTG AGGAAATATG TCTTGTTGAT GAGAATCAGT TTACATTGAC GATTGCCAAC
    CAGGGAACGC CACTCACCTT CATGCATCAG GAGTGTGAAG CCATTGTGAG GTCCATTATT
    CATATACGGA CACGGTGGGA GCTGTCGCAA CCAGACTCCA TCCCACAGCA CACTAAAATT
    CGCCCTAAGG ATGTTCCCGG AACACTACTG AACATAGCAT TGCTCAACCT GGGAAGCTCA
    GACCCGAGTT TGCGGTCTGC TGCCTATAAT CTTCTGTGTG CCTTAACCTG TACCTTTAAT
    TTAAAGATTG AGGGCCAGTT ACTGGAAACT TCAGGTCTGT GTATTCCTGC CAACAACACG
    CTCTTTATTG TGTCTATTAG TAAGACTCTT GCAGCTAATG AGCCACACCT TACCCTAGAG
    TTCTTGGAAG AGTGTATTTC AGGATTTAGC AAATCTAGTA TTGAACTGAA ACATCTTTGT
    CTGGAATATA TGACTCCCTG GCTGTCCAAT CTGGTCCGAT TCTGCAAACA CAACGACGAT
    GCGAAGCGCC AGCGAGTCAC TGCCATTCTT GATAAGCTTA TAACAATGAC AATAAATGAA
    AAACAGATGT ACCCTTCTAT TCAGGCTAAA ATATGGGGAA GTCTTGGACA GATTACAGAT
    CTCCTTGACG TAGTGCTGGA CAGTTTCATC AAAACTAGTG CCACGGGGGG CTTAGGATCC
    ATTAAAGCTG AGGTTATGGC AGACACAGCA GTAGCGCTTG CTTCTGGAAA TGTAAAATTG
    GTTTCAAGCA AAGTGATTGG AAGGATGTGC AAAATAATTG ACAAGACTTG TCTGTCTCCA
    ACTCCTACAC TGGAACAGCA CCTGATGTGG GATGATATTG CCATTCTAGC ACGTTACATG
    CTGATGCTCT CCTTCAACAA TTCTCTGGAT GTGGCTGCAC ATCTCCCCTA CCTCTTTCAT
    GTGGTTACTT TGTTGGTGGC CACTGGACCC CTTTCTCTCA GAGCTTCCAC TCATGGTCTT
    GTCATCAACA TCATTCATTC TCTGTGCACT TGCTCACAGC TTCATTTTAG TGAGGAGACC
    AAGCAAGTTT TAAGACTGAG CTTGACTGAA TTTTCTCTGC CTAAATTTTA TTTGCTGTTT
    GGAATCAGCA AAGTGAAATC AGCTGCAGTC ATAGCATTCC GCTCCAGCTA TCGAGACAGG
    TCATTTTCAC CTGGCTCCTA CGAGAGGGAG ACTTTTGCAC TGACCTCCTT GGAAACTGTT
    ACTGAAGCTT TGCTGGAGAT CATGGAGGCT TGTATGAGAG ATATTCCAAC ATGCAAGTGG
    CTGGACCAGT GGACCGAACT AGCACAAAAG TTTGCATTCC AGTACAATCC ATCCCTGCAG
    CCAAGAGCAC TCGTTGTCTT TGGCTGTATA AGTAAGCGTG TCTCTCATGG ACAAATAAAA
    CAAATAATCC GAATTCTTAG CAAGGGGCTT GAGAGCTGTT TAAAAGGCCC TGATAATTAC
    AATAGCCAAG TTCTAATAGA AGCTACGGTT ATAGCACTCA CCAAACTGCA GCCTCTTCTT
    AACAAGGACT CCCCCATGCA CAAGGCCCTC TTCTGGGTAG CCATGGCAGT ACTGCAGTTG
    GATGAAGTCA ACCTATACTC AGCAGGTACA GCACTGCTTG AACAGAATCT GCATACCTTG
    GATAGCCTTC GAGTGTTCAA TGATAAGAGC CCAGAAGAGG TGTTCATGGA GATCAGGAGA
    CCACTTGAAT GGCACTGCAA ACAGATGGAT CATTTTGTTG GGCTAAATTT CAACTCCAAC
    TTCAACTTTG CACTAGTGGG GCACCTCCTG AAAGGGTACA GGCATCCTTC ACCTACCACT
    GTAGCAAGAA CAGTAAGGAT TCTACATACA CTACTAGCTC TGGTTAACAA ACACAGGAAC
    TGTGACAAGT TTGAGGTGAA CACCCAGAGC GTGGCTTACC TAGCAGCTTT GCTGACAGTG
    TCTGAGGAAG TTCGAAGTCG CTGCAGCCTA AAACATAGGA AGTCTCTCTT GCTGACAGAT
    GTTGCAATGG AAAATGTTCC TATGGATACA TATCCCATAC ATCACAGTGA CACTACCTAT
    AGGACACTAA AGGAAAATCA ACCATGGTCA TCACCAAAGG GATCAGACAG ACACCTGGCT
    GCCAGTTACC CGACAGTGGG ACAGATAAGC CCTCGAACAC GAAAGTCCAT GAGTTTGGAT
    ATGGGGCAGC CTTCACAAGC GAACACTAAA AAGCTTCTAG GTACAAGGAA AAGTTTTGAC
    CATTTGATAT CGGACACAAA GGCTCCTAAA AGGCAAGACA TGGAATCTGG AATCACAACG
    CCTCCCAAAA TGAGGAGAGT AGCCGAAAAT GATTATGAAA TGGAAACCCA GAGAATATCA
    CCGCAGCAGC ACCCCCATCT CCGGAAAGTT TCTGTTTCTG AGTCAAATGT CCTCCTGGAT
    GAAGAAGTTC TGACTGATCC AAAAATTCAA GCACTGCTGC TTACAGTTCT TGCCACGTTA
    GTGAAGTACA CTACTGATGA ATTTGACCAG CGGATTCTGT ATGAGTATTT AGCAGAAGCC
    AGTGTTGTCT TCCCAAAGGT CTTTCCTGTT GTGCATAATT TATTGGATTC CAAGATCAAT
    ACCCTCTTGT CGCTGTGTCA AGATCCAAAT TTGTTGAATC CAATTCATGG GATTGTTCAG
    AGTGTTGTCT ACCATGAGGA GTCTCCACCC CAATACCAAA CTTCTTATCT ACAAAGTTTT
    GGATTTAATG GGTTGTGGAG GTTTGCTGGC CCATTTTCCA AGCAAACACA AATCCCAGAC
    TATGCTGAGC TCATAGTGAA GTTTCTTGAT GCCTTGATTG ACACGTATTT GCCTGGAATT
    GATGAGGAAA CCAGTGAAGA ATCTCTCCTG ACACCCACGT CTCCATATCC TCCCGCAGTG
    CAGAGCCAGC TTAGCATTAC AGCTAACCTT AACCTTTCCA ATTCCATGAC CTCGCTCGCA
    ACTTCCCAGC ACTCCCCAGG GATCGACAAG GAGAACGTGG AGCTCTCGCC CACCACTGGC
    CACACCAACA GCGGGAGGAT GCGCCACGGC TCCGCCAGCC AGGTGCAAAA GCAGCGAAGT
    GCTGGCAGCT TGAAACGTCA CAGCATTAAG AAGATTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_415914.2
    CDS1..8502
    Translation

    Target ORF information:

    RefSeq Version XM_415914.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofibromin 1 (NF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_415914.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGAGTCTGAC ATTTAAAGAG AAAATGACAA GCCTTAAATT TAAAGAAAAG
    CCTACTGACT TGGAAACCAA AAGCTACAAG TTCCTGCTGT TATCCTTGGT AAAACTGATC
    CATGCTGATC CAAAGCTTTT GCTGTGTAAT CCAAGGAAGC AAGGGCCTGA GACACAAGGC
    AGCACTGCTG AATTAATTAC AGGCCTTGTA CAACTCGTTC CACAATCCAG CATGCCAGAT
    ATAGCACAAG AAGCCATGGA GGCCTTGCTA GTTCTTCACC AGTTAGACAG CATTGACTTA
    TGGAATCCTG ATGCACCTAT AGAAACTTTC TGGGAAATTA GTTCACAAAT GCTTTTTTAT
    ATCTGCAAGA AATTAACCAG TCATCAGATG CTCAGCAGTA CAGAAATCCT CAAGTGGCTG
    CGAGAGATTC TCATCTGTAG GAATAAATTT CTTCTAAAAA ATAAACAATC AGACAGAACT
    TCCTGTCACT TTCTCTTCCT TTATGATGTC TCTGGAGGTG GAGCTAGTCA GATGTCCCTT
    GACCACGAAG AGATGATGCG CTGTGCTCCC GGAGCCACCC TCAGAAAAGG AAAAGGGAAT
    TCTTCCATGG AAAGTACAGC TGGATGCAGT GGAACACCCA TTTGTCGCCA GGCCCAAACA
    AAATTGGAAG TTGCCTTATA CATGTTTTTG TGGAGTCCGG ATATTGAGGC AGTCCTTGTT
    GCAATGTCCT GTTTCCGTCA CCTCTGTGAG GAAGCAGATA TCAGATGTGG AGTAGATGAA
    GTTTCAGTTC ATAATTTTTT GCCAAACTAC AACACTTTCA TGGAGTTTGC ATCTGTTAGC
    AATATGATGT CAACAGGGAG AGCAGCTCTT CAGAAAAGAG TGATGGCTTT ATTAAGACGT
    ATTGAACATC CAACTGCAGG GAACACAGAG GCTTGGGAGG ATACACATGC AAAATGGGAA
    CAAGCTACAA AACTAATCCT TAACTATCCA AAGACCAAAA TGGAAGACGG GCAGGTTACT
    GAAAGTCTTC ATAAGACAAT TGTTAAGAGA CGAATGTCAC ATGTTAGTGG GGGAGGCTCC
    ATAGACTTGT CTGACACAGA TTCTCTTCAG GAATGGATCA ACATGACGGG ATTCCTCTGT
    GCCCTCGGAG GAGTGTGCCT GCAGCATCGT AGCAATGCAG GATTAGCAAC CTACAGTCCA
    CCCATGGGCC CAGTCAATGA GCGTAAGGGC TCCATGATAT CTATGGTATC CACTGAGGGA
    AATGCAGAGA CTCCTGTTAG CAAATTCATA GATCGATTAC TGACTCTGAT GGTCTGTAAC
    CATGAGAAAG TGGGACTACA AATACGGACT AATATTAAAG ATCTGGTGGG TTTGGAGTTG
    AGTCCAGCAC TTTATCCAAT GCTATTTAAC AAATTGAAGA ATACCATCAG CAAGTTTTTT
    GATTCTCAAG GACAGGTTTT GTTAACTGAG ACCAACACAC AGTTTGTAGA ACAAACCATT
    GCTATAATGA AGAACTTGCT TGACAATCAT ACAGAAGGGA GCTCAGAACA TCTTGGACAA
    GCTAGTATTG AGACAATGAT GCTAAACCTG GTTAGGTATG TGCGTGTACT TGGAAATTTG
    GTACATGCAA TTCAAATAAA AACTAAACTC TGTCAGTTGG TGGAAGTAAT GATGGAGCGG
    AGAGATGACC TTTCATTTTG CCAAGAAATG AAATTTAGGA ACAAAATGGT GGAATATCTG
    ACAGACTGGG TTATGGGAAC ATCTAATCAA GCAACAGATG AAGATGTGAA GTGCTTGACA
    AGAGATTTGG ACCAAGCAAG TATGGAAGCA GTGGTCTCTC TTCTTGCTGG GCTTCCGTTA
    CAGCCTGAAG AAGGAGATGG TGTTGAGTTG ATGGAAGCCA AATCTCAGTT ATTTCTTAAA
    TATTTCACAC TGTTTATGAA CCTTCTAAAT GACTGTAGTG AAGTTGAAGA TGAAAGTGCA
    CAAACAGGTG GCAGGAAACG TGGGATGTCC CGAAGACTGG CTTCGCTGCG GCACTGCACT
    GTTCTTGCTA TGTCAAATTT ACTAAATGCA AATGTGGACA GTGGTCTTAT GCATTCAATA
    GGCTTGGGCT ATCATAAAGA CCTCCAGACA AGAGCTACAT TTATGGAAGT CCTCACCAAA
    ATTCTACAGC AGGGAACAGA ATTTGATACG TTAGCAGAAA CGGTGCTGGC AGATCGGTTT
    GAGAGGCTGG TGGAGCTAGT TACAATGATG GGTGATCAGG GGGAGCTTCC TATTGCTATG
    GCTTTAGCAA ATGTGGTGCC TTGTTCTCAG TGGGATGAGC TAGCTCGTGT TCTGGTCACA
    CTCTTTGATT CCCGGCACTT GCTCTACCAG CTACTTTGGA ATATGTTTTC AAAGGAAGTT
    GAACTGGCAG ATTCCATGCA GACACTCTTC CGAGGAAACA GCTTAGCTAG TAAAATAATG
    ACTTTCTGTT TTAAGGTATA TGGTGCTACT TATTTGCAGA AGCTTTTGGA ACCTTTATTA
    AGAACAGTGA TCACATCCTC TGAATGGCAG CATGTTAGCT TTGAGGTGGA TCCTACAAGA
    CTGGAGCCTA CAGAAAGCCT TGAAGAGAAC CAGCGGAGTC TCTTGCAAAT GACAGAAAAA
    TTTTTTCATG CAATCATTAA TTCTTCTTCG GAATTCCCAC CACAACTTCG CAGTGTGTGC
    CATTGTTTGT ACCAGGCAAC TTGCCACTCT CTACTGAATA AAGCTACCGT AAAAGAAAAA
    AAGGAAAACA AAAAATCAGT TGTTAGTCAG CGATTTCCTC AGAACAGCAT TGGAGCAGTT
    GGGAGTGCCA TGTTCCTGCG GTTCATCAAC CCTGCGATCG TCTCCCCGTA CGAGGCAGGG
    ATTTTAGATA AAAAGCCTCC GCCCAGAATT GAGAGGGGAC TGAAACTGAT GTCAAAGATA
    CTTCAGAGTA TTGCCAACCA CGTCCTGTTT ACAAAAGAAG AACATATGAG GCCTTTTAAT
    GACTTCGTAA AAAGCAATTT TGATGCAGCT CGAAGGTTTT TCCTTGATAT TGCATCTGAT
    TGCCCTGCGA GTGACACTGT CAATCACAGC CTGTCTTTCA TCAGTGATGG CAATGTTCTT
    GCTTTGCACC GGTTGTTGTG GAACAATCAG GAGAAAATTG GCCAGTACCT TTCCAGTAAT
    AGGGACCACA AAGCTGTTGG AAGGCGACCT TTTGACAAAA TGGCTACTCT TCTTGCCTAT
    CTGGGTCCAC CAGAGCACAA ACCTGTGGCA GATACACATT GGTCCAGTTT AAACCTCACC
    AGTTCGAAAT TTGAAGAATT TATGACAAGG CATCAGGTAC ATGAAAAAGA AGAGTTCAAA
    GCATTGAAAA CGTTAAATAT TTTCTACCAG GCTGGGACAT CCAAAGCTGG CAATCCTGTT
    TTCTACTACA TTGCTCGGCG TTTCAAGACT GGTCAGATCA ATGGAGATTT ATTGATATAC
    CACGTCCTGC TAACTCTGAA GCCATACTAC GCAAAGCCAT ATGAAATTGT AGTGGACCTC
    ACACATGCTG GCCCCAGTAA TCGCTTTAAA ACAGACTTTC TTTCTAAATG GTTTGTAGTT
    TTTCCAGGCT TTGCTTATGA AAATGTCTCA GCAGTTTTTA TTTATAACTG TAATTCTTGG
    GTCAGAGAAT ACACCAAATA CCACGAAAGA CTCTTGACAG GTTTGAAAGG AAGCAAAAGG
    CTTATTTTCA TAGACTCTCC AGGAAAACTG GCGGAGCACA TTGAACATGA CCAGCAGAAA
    CTTCCAGCAG CTACCTTAGC TTTGGAAGAG GACTTGAAAG TATTTCACAA TGCCCTCAAA
    TTGGCTCACA AAGACACCAA AGTGTCTATT AAAGTTGGAT CAACAGCTGT GCAGGTGACA
    TCAGCAGAAC GAACAAGAGT CCTGGGACAG ACAGTGTTTT TAAATGATAT ATACTACGCC
    TCTGAAATTG AGGAAATATG TCTTGTTGAT GAGAATCAGT TTACATTGAC GATTGCCAAC
    CAGGGAACGC CACTCACCTT CATGCATCAG GAGTGTGAAG CCATTGTGAG GTCCATTATT
    CATATACGGA CACGGTGGGA GCTGTCGCAA CCAGACTCCA TCCCACAGCA CACTAAAATT
    CGCCCTAAGG ATGTTCCCGG AACACTACTG AACATAGCAT TGCTCAACCT GGGAAGCTCA
    GACCCGAGTT TGCGGTCTGC TGCCTATAAT CTTCTGTGTG CCTTAACCTG TACCTTTAAT
    TTAAAGATTG AGGGCCAGTT ACTGGAAACT TCAGGTCTGT GTATTCCTGC CAACAACACG
    CTCTTTATTG TGTCTATTAG TAAGACTCTT GCAGCTAATG AGCCACACCT TACCCTAGAG
    TTCTTGGAAG AGTGTATTTC AGGATTTAGC AAATCTAGTA TTGAACTGAA ACATCTTTGT
    CTGGAATATA TGACTCCCTG GCTGTCCAAT CTGGTCCGAT TCTGCAAACA CAACGACGAT
    GCGAAGCGCC AGCGAGTCAC TGCCATTCTT GATAAGCTTA TAACAATGAC AATAAATGAA
    AAACAGATGT ACCCTTCTAT TCAGGCTAAA ATATGGGGAA GTCTTGGACA GATTACAGAT
    CTCCTTGACG TAGTGCTGGA CAGTTTCATC AAAACTAGTG CCACGGGGGG CTTAGGATCC
    ATTAAAGCTG AGGTTATGGC AGACACAGCA GTAGCGCTTG CTTCTGGAAA TGTAAAATTG
    GTTTCAAGCA AAGTGATTGG AAGGATGTGC AAAATAATTG ACAAGACTTG TCTGTCTCCA
    ACTCCTACAC TGGAACAGCA CCTGATGTGG GATGATATTG CCATTCTAGC ACGTTACATG
    CTGATGCTCT CCTTCAACAA TTCTCTGGAT GTGGCTGCAC ATCTCCCCTA CCTCTTTCAT
    GTGGTTACTT TGTTGGTGGC CACTGGACCC CTTTCTCTCA GAGCTTCCAC TCATGGTCTT
    GTCATCAACA TCATTCATTC TCTGTGCACT TGCTCACAGC TTCATTTTAG TGAGGAGACC
    AAGCAAGTTT TAAGACTGAG CTTGACTGAA TTTTCTCTGC CTAAATTTTA TTTGCTGTTT
    GGAATCAGCA AAGTGAAATC AGCTGCAGTC ATAGCATTCC GCTCCAGCTA TCGAGACAGG
    TCATTTTCAC CTGGCTCCTA CGAGAGGGAG ACTTTTGCAC TGACCTCCTT GGAAACTGTT
    ACTGAAGCTT TGCTGGAGAT CATGGAGGCT TGTATGAGAG ATATTCCAAC ATGCAAGTGG
    CTGGACCAGT GGACCGAACT AGCACAAAAG TTTGCATTCC AGTACAATCC ATCCCTGCAG
    CCAAGAGCAC TCGTTGTCTT TGGCTGTATA AGTAAGCGTG TCTCTCATGG ACAAATAAAA
    CAAATAATCC GAATTCTTAG CAAGGGGCTT GAGAGCTGTT TAAAAGGCCC TGATAATTAC
    AATAGCCAAG TTCTAATAGA AGCTACGGTT ATAGCACTCA CCAAACTGCA GCCTCTTCTT
    AACAAGGACT CCCCCATGCA CAAGGCCCTC TTCTGGGTAG CCATGGCAGT ACTGCAGTTG
    GATGAAGTCA ACCTATACTC AGCAGGTACA GCACTGCTTG AACAGAATCT GCATACCTTG
    GATAGCCTTC GAGTGTTCAA TGATAAGAGC CCAGAAGAGG TGTTCATGGA GATCAGGAGA
    CCACTTGAAT GGCACTGCAA ACAGATGGAT CATTTTGTTG GGCTAAATTT CAACTCCAAC
    TTCAACTTTG CACTAGTGGG GCACCTCCTG AAAGGGTACA GGCATCCTTC ACCTACCACT
    GTAGCAAGAA CAGTAAGGAT TCTACATACA CTACTAGCTC TGGTTAACAA ACACAGGAAC
    TGTGACAAGT TTGAGGTGAA CACCCAGAGC GTGGCTTACC TAGCAGCTTT GCTGACAGTG
    TCTGAGGAAG TTCGAAGTCG CTGCAGCCTA AAACATAGGA AGTCTCTCTT GCTGACAGAT
    GTTGCAATGG AAAATGTTCC TATGGATACA TATCCCATAC ATCACAGTGA CACTACCTAT
    AGGACACTAA AGGAAAATCA ACCATGGTCA TCACCAAAGG GATCAGACAG ACACCTGGCT
    GCCAGTTACC CGACAGTGGG ACAGATAAGC CCTCGAACAC GAAAGTCCAT GAGTTTGGAT
    ATGGGGCAGC CTTCACAAGC GAACACTAAA AAGCTTCTAG GTACAAGGAA AAGTTTTGAC
    CATTTGATAT CGGACACAAA GGCTCCTAAA AGGCAAGACA TGGAATCTGG AATCACAACG
    CCTCCCAAAA TGAGGAGAGT AGCCGAAAAT GATTATGAAA TGGAAACCCA GAGAATATCA
    CCGCAGCAGC ACCCCCATCT CCGGAAAGTT TCTGTTTCTG AGTCAAATGT CCTCCTGGAT
    GAAGAAGTTC TGACTGATCC AAAAATTCAA GCACTGCTGC TTACAGTTCT TGCCACGTTA
    GTGAAGTACA CTACTGATGA ATTTGACCAG CGGATTCTGT ATGAGTATTT AGCAGAAGCC
    AGTGTTGTCT TCCCAAAGGT CTTTCCTGTT GTGCATAATT TATTGGATTC CAAGATCAAT
    ACCCTCTTGT CGCTGTGTCA AGATCCAAAT TTGTTGAATC CAATTCATGG GATTGTTCAG
    AGTGTTGTCT ACCATGAGGA GTCTCCACCC CAATACCAAA CTTCTTATCT ACAAAGTTTT
    GGATTTAATG GGTTGTGGAG GTTTGCTGGC CCATTTTCCA AGCAAACACA AATCCCAGAC
    TATGCTGAGC TCATAGTGAA GTTTCTTGAT GCCTTGATTG ACACGTATTT GCCTGGAATT
    GATGAGGAAA CCAGTGAAGA ATCTCTCCTG ACACCCACGT CTCCATATCC TCCCGCAGTG
    CAGAGCCAGC TTAGCATTAC AGCTAACCTT AACCTTTCCA ATTCCATGAC CTCGCTCGCA
    ACTTCCCAGC ACTCCCCAGG GATCGACAAG GAGAACGTGG AGCTCTCGCC CACCACTGGC
    CACACCAACA GCGGGAGGAT GCGCCACGGC TCCGCCAGCC AGGTGCAAAA GCAGCGAAGT
    GCTGGCAGCT TGAAACGTCA CAGCATTAAG AAGATTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17416
    Clone ID Related Accession (Same CDS sequence) XM_415914.5 , XM_415914.6
    Accession Version XM_415914.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofibromin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_415914.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGAGTCTGAC ATTTAAAGAG AAAATGACAA GCCTTAAATT TAAAGAAAAG
    CCTACTGACT TGGAAACCAA AAGCTACAAG TTCCTGCTGT TATCCTTGGT AAAACTGATC
    CATGCTGATC CAAAGCTTTT GCTGTGTAAT CCAAGGAAGC AAGGGCCTGA GACACAAGGC
    AGCACTGCTG AATTAATTAC AGGCCTTGTA CAACTCGTTC CACAATCCAG CATGCCAGAT
    ATAGCACAAG AAGCCATGGA GGCCTTGCTA GTTCTTCACC AGTTAGACAG CATTGACTTA
    TGGAATCCTG ATGCACCTAT AGAAACTTTC TGGGAAATTA GTTCACAAAT GCTTTTTTAT
    ATCTGCAAGA AATTAACCAG TCATCAGATG CTCAGCAGTA CAGAAATCCT CAAGTGGCTG
    CGAGAGATTC TCATCTGTAG GAATAAATTT CTTCTAAAAA ATAAACAATC AGACAGAACT
    TCCTGTCACT TTCTCTTCCT TTATGATGTC TCTGGAGGTG GAGCTAGTCA GATGTCCCTT
    GACCACGAAG AGATGATGCG CTGTGCTCCC GGAGCCACCC TCAGAAAAGG AAAAGGGAAT
    TCTTCCATGG AAAGTACAGC TGGATGCAGT GGAACACCCA TTTGTCGCCA GGCCCAAACA
    AAATTGGAAG TTGCCTTATA CATGTTTTTG TGGAGTCCGG ATATTGAGGC AGTCCTTGTT
    GCAATGTCCT GTTTCCGTCA CCTCTGTGAG GAAGCAGATA TCAGATGTGG AGTAGATGAA
    GTTTCAGTTC ATAATTTTTT GCCAAACTAC AACACTTTCA TGGAGTTTGC ATCTGTTAGC
    AATATGATGT CAACAGGGAG AGCAGCTCTT CAGAAAAGAG TGATGGCTTT ATTAAGACGT
    ATTGAACATC CAACTGCAGG GAACACAGAG GCTTGGGAGG ATACACATGC AAAATGGGAA
    CAAGCTACAA AACTAATCCT TAACTATCCA AAGACCAAAA TGGAAGACGG GCAGGTTACT
    GAAAGTCTTC ATAAGACAAT TGTTAAGAGA CGAATGTCAC ATGTTAGTGG GGGAGGCTCC
    ATAGACTTGT CTGACACAGA TTCTCTTCAG GAATGGATCA ACATGACGGG ATTCCTCTGT
    GCCCTCGGAG GAGTGTGCCT GCAGCATCGT AGCAATGCAG GATTAGCAAC CTACAGTCCA
    CCCATGGGCC CAGTCAATGA GCGTAAGGGC TCCATGATAT CTATGGTATC CACTGAGGGA
    AATGCAGAGA CTCCTGTTAG CAAATTCATA GATCGATTAC TGACTCTGAT GGTCTGTAAC
    CATGAGAAAG TGGGACTACA AATACGGACT AATATTAAAG ATCTGGTGGG TTTGGAGTTG
    AGTCCAGCAC TTTATCCAAT GCTATTTAAC AAATTGAAGA ATACCATCAG CAAGTTTTTT
    GATTCTCAAG GACAGGTTTT GTTAACTGAG ACCAACACAC AGTTTGTAGA ACAAACCATT
    GCTATAATGA AGAACTTGCT TGACAATCAT ACAGAAGGGA GCTCAGAACA TCTTGGACAA
    GCTAGTATTG AGACAATGAT GCTAAACCTG GTTAGGTATG TGCGTGTACT TGGAAATTTG
    GTACATGCAA TTCAAATAAA AACTAAACTC TGTCAGTTGG TGGAAGTAAT GATGGAGCGG
    AGAGATGACC TTTCATTTTG CCAAGAAATG AAATTTAGGA ACAAAATGGT GGAATATCTG
    ACAGACTGGG TTATGGGAAC ATCTAATCAA GCAACAGATG AAGATGTGAA GTGCTTGACA
    AGAGATTTGG ACCAAGCAAG TATGGAAGCA GTGGTCTCTC TTCTTGCTGG GCTTCCGTTA
    CAGCCTGAAG AAGGAGATGG TGTTGAGTTG ATGGAAGCCA AATCTCAGTT ATTTCTTAAA
    TATTTCACAC TGTTTATGAA CCTTCTAAAT GACTGTAGTG AAGTTGAAGA TGAAAGTGCA
    CAAACAGGTG GCAGGAAACG TGGGATGTCC CGAAGACTGG CTTCGCTGCG GCACTGCACT
    GTTCTTGCTA TGTCAAATTT ACTAAATGCA AATGTGGACA GTGGTCTTAT GCATTCAATA
    GGCTTGGGCT ATCATAAAGA CCTCCAGACA AGAGCTACAT TTATGGAAGT CCTCACCAAA
    ATTCTACAGC AGGGAACAGA ATTTGATACG TTAGCAGAAA CGGTGCTGGC AGATCGGTTT
    GAGAGGCTGG TGGAGCTAGT TACAATGATG GGTGATCAGG GGGAGCTTCC TATTGCTATG
    GCTTTAGCAA ATGTGGTGCC TTGTTCTCAG TGGGATGAGC TAGCTCGTGT TCTGGTCACA
    CTCTTTGATT CCCGGCACTT GCTCTACCAG CTACTTTGGA ATATGTTTTC AAAGGAAGTT
    GAACTGGCAG ATTCCATGCA GACACTCTTC CGAGGAAACA GCTTAGCTAG TAAAATAATG
    ACTTTCTGTT TTAAGGTATA TGGTGCTACT TATTTGCAGA AGCTTTTGGA ACCTTTATTA
    AGAACAGTGA TCACATCCTC TGAATGGCAG CATGTTAGCT TTGAGGTGGA TCCTACAAGA
    CTGGAGCCTA CAGAAAGCCT TGAAGAGAAC CAGCGGAGTC TCTTGCAAAT GACAGAAAAA
    TTTTTTCATG CAATCATTAA TTCTTCTTCG GAATTCCCAC CACAACTTCG CAGTGTGTGC
    CATTGTTTGT ACCAGGCAAC TTGCCACTCT CTACTGAATA AAGCTACCGT AAAAGAAAAA
    AAGGAAAACA AAAAATCAGT TGTTAGTCAG CGATTTCCTC AGAACAGCAT TGGAGCAGTT
    GGGAGTGCCA TGTTCCTGCG GTTCATCAAC CCTGCGATCG TCTCCCCGTA CGAGGCAGGG
    ATTTTAGATA AAAAGCCTCC GCCCAGAATT GAGAGGGGAC TGAAACTGAT GTCAAAGATA
    CTTCAGAGTA TTGCCAACCA CGTCCTGTTT ACAAAAGAAG AACATATGAG GCCTTTTAAT
    GACTTCGTAA AAAGCAATTT TGATGCAGCT CGAAGGTTTT TCCTTGATAT TGCATCTGAT
    TGCCCTGCGA GTGACACTGT CAATCACAGC CTGTCTTTCA TCAGTGATGG CAATGTTCTT
    GCTTTGCACC GGTTGTTGTG GAACAATCAG GAGAAAATTG GCCAGTACCT TTCCAGTAAT
    AGGGACCACA AAGCTGTTGG AAGGCGACCT TTTGACAAAA TGGCTACTCT TCTTGCCTAT
    CTGGGTCCAC CAGAGCACAA ACCTGTGGCA GATACACATT GGTCCAGTTT AAACCTCACC
    AGTTCGAAAT TTGAAGAATT TATGACAAGG CATCAGGTAC ATGAAAAAGA AGAGTTCAAA
    GCATTGAAAA CGTTAAATAT TTTCTACCAG GCTGGGACAT CCAAAGCTGG CAATCCTGTT
    TTCTACTACA TTGCTCGGCG TTTCAAGACT GGTCAGATCA ATGGAGATTT ATTGATATAC
    CACGTCCTGC TAACTCTGAA GCCATACTAC GCAAAGCCAT ATGAAATTGT AGTGGACCTC
    ACACATGCTG GCCCCAGTAA TCGCTTTAAA ACAGACTTTC TTTCTAAATG GTTTGTAGTT
    TTTCCAGGCT TTGCTTATGA AAATGTCTCA GCAGTTTTTA TTTATAACTG TAATTCTTGG
    GTCAGAGAAT ACACCAAATA CCACGAAAGA CTCTTGACAG GTTTGAAAGG AAGCAAAAGG
    CTTATTTTCA TAGACTCTCC AGGAAAACTG GCGGAGCACA TTGAACATGA CCAGCAGAAA
    CTTCCAGCAG CTACCTTAGC TTTGGAAGAG GACTTGAAAG TATTTCACAA TGCCCTCAAA
    TTGGCTCACA AAGACACCAA AGTGTCTATT AAAGTTGGAT CAACAGCTGT GCAGGTGACA
    TCAGCAGAAC GAACAAGAGT CCTGGGACAG ACAGTGTTTT TAAATGATAT ATACTACGCC
    TCTGAAATTG AGGAAATATG TCTTGTTGAT GAGAATCAGT TTACATTGAC GATTGCCAAC
    CAGGGAACGC CACTCACCTT CATGCATCAG GAGTGTGAAG CCATTGTGAG GTCCATTATT
    CATATACGGA CACGGTGGGA GCTGTCGCAA CCAGACTCCA TCCCACAGCA CACTAAAATT
    CGCCCTAAGG ATGTTCCCGG AACACTACTG AACATAGCAT TGCTCAACCT GGGAAGCTCA
    GACCCGAGTT TGCGGTCTGC TGCCTATAAT CTTCTGTGTG CCTTAACCTG TACCTTTAAT
    TTAAAGATTG AGGGCCAGTT ACTGGAAACT TCAGGTCTGT GTATTCCTGC CAACAACACG
    CTCTTTATTG TGTCTATTAG TAAGACTCTT GCAGCTAATG AGCCACACCT TACCCTAGAG
    TTCTTGGAAG AGTGTATTTC AGGATTTAGC AAATCTAGTA TTGAACTGAA ACATCTTTGT
    CTGGAATATA TGACTCCCTG GCTGTCCAAT CTGGTCCGAT TCTGCAAACA CAACGACGAT
    GCGAAGCGCC AGCGAGTCAC TGCCATTCTT GATAAGCTTA TAACAATGAC AATAAATGAA
    AAACAGATGT ACCCTTCTAT TCAGGCTAAA ATATGGGGAA GTCTTGGACA GATTACAGAT
    CTCCTTGACG TAGTGCTGGA CAGTTTCATC AAAACTAGTG CCACGGGGGG CTTAGGATCC
    ATTAAAGCTG AGGTTATGGC AGACACAGCA GTAGCGCTTG CTTCTGGAAA TGTAAAATTG
    GTTTCAAGCA AAGTGATTGG AAGGATGTGC AAAATAATTG ACAAGACTTG TCTGTCTCCA
    ACTCCTACAC TGGAACAGCA CCTGATGTGG GATGATATTG CCATTCTAGC ACGTTACATG
    CTGATGCTCT CCTTCAACAA TTCTCTGGAT GTGGCTGCAC ATCTCCCCTA CCTCTTTCAT
    GTGGTTACTT TGTTGGTGGC CACTGGACCC CTTTCTCTCA GAGCTTCCAC TCATGGTCTT
    GTCATCAACA TCATTCATTC TCTGTGCACT TGCTCACAGC TTCATTTTAG TGAGGAGACC
    AAGCAAGTTT TAAGACTGAG CTTGACTGAA TTTTCTCTGC CTAAATTTTA TTTGCTGTTT
    GGAATCAGCA AAGTGAAATC AGCTGCAGTC ATAGCATTCC GCTCCAGCTA TCGAGACAGG
    TCATTTTCAC CTGGCTCCTA CGAGAGGGAG ACTTTTGCAC TGACCTCCTT GGAAACTGTT
    ACTGAAGCTT TGCTGGAGAT CATGGAGGCT TGTATGAGAG ATATTCCAAC ATGCAAGTGG
    CTGGACCAGT GGACCGAACT AGCACAAAAG TTTGCATTCC AGTACAATCC ATCCCTGCAG
    CCAAGAGCAC TCGTTGTCTT TGGCTGTATA AGTAAGCGTG TCTCTCATGG ACAAATAAAA
    CAAATAATCC GAATTCTTAG CAAGGGGCTT GAGAGCTGTT TAAAAGGCCC TGATAATTAC
    AATAGCCAAG TTCTAATAGA AGCTACGGTT ATAGCACTCA CCAAACTGCA GCCTCTTCTT
    AACAAGGACT CCCCCATGCA CAAGGCCCTC TTCTGGGTAG CCATGGCAGT ACTGCAGTTG
    GATGAAGTCA ACCTATACTC AGCAGGTACA GCACTGCTTG AACAGAATCT GCATACCTTG
    GATAGCCTTC GAGTGTTCAA TGATAAGAGC CCAGAAGAGG TGTTCATGGA GATCAGGAGA
    CCACTTGAAT GGCACTGCAA ACAGATGGAT CATTTTGTTG GGCTAAATTT CAACTCCAAC
    TTCAACTTTG CACTAGTGGG GCACCTCCTG AAAGGGTACA GGCATCCTTC ACCTACCACT
    GTAGCAAGAA CAGTAAGGAT TCTACATACA CTACTAGCTC TGGTTAACAA ACACAGGAAC
    TGTGACAAGT TTGAGGTGAA CACCCAGAGC GTGGCTTACC TAGCAGCTTT GCTGACAGTG
    TCTGAGGAAG TTCGAAGTCG CTGCAGCCTA AAACATAGGA AGTCTCTCTT GCTGACAGAT
    GTTGCAATGG AAAATGTTCC TATGGATACA TATCCCATAC ATCACAGTGA CACTACCTAT
    AGGACACTAA AGGAAAATCA ACCATGGTCA TCACCAAAGG GATCAGACAG ACACCTGGCT
    GCCAGTTACC CGACAGTGGG ACAGATAAGC CCTCGAACAC GAAAGTCCAT GAGTTTGGAT
    ATGGGGCAGC CTTCACAAGC GAACACTAAA AAGCTTCTAG GTACAAGGAA AAGTTTTGAC
    CATTTGATAT CGGACACAAA GGCTCCTAAA AGGCAAGACA TGGAATCTGG AATCACAACG
    CCTCCCAAAA TGAGGAGAGT AGCCGAAAAT GATTATGAAA TGGAAACCCA GAGAATATCA
    CCGCAGCAGC ACCCCCATCT CCGGAAAGTT TCTGTTTCTG AGTCAAATGT CCTCCTGGAT
    GAAGAAGTTC TGACTGATCC AAAAATTCAA GCACTGCTGC TTACAGTTCT TGCCACGTTA
    GTGAAGTACA CTACTGATGA ATTTGACCAG CGGATTCTGT ATGAGTATTT AGCAGAAGCC
    AGTGTTGTCT TCCCAAAGGT CTTTCCTGTT GTGCATAATT TATTGGATTC CAAGATCAAT
    ACCCTCTTGT CGCTGTGTCA AGATCCAAAT TTGTTGAATC CAATTCATGG GATTGTTCAG
    AGTGTTGTCT ACCATGAGGA GTCTCCACCC CAATACCAAA CTTCTTATCT ACAAAGTTTT
    GGATTTAATG GGTTGTGGAG GTTTGCTGGC CCATTTTCCA AGCAAACACA AATCCCAGAC
    TATGCTGAGC TCATAGTGAA GTTTCTTGAT GCCTTGATTG ACACGTATTT GCCTGGAATT
    GATGAGGAAA CCAGTGAAGA ATCTCTCCTG ACACCCACGT CTCCATATCC TCCCGCAGTG
    CAGAGCCAGC TTAGCATTAC AGCTAACCTT AACCTTTCCA ATTCCATGAC CTCGCTCGCA
    ACTTCCCAGC ACTCCCCAGG GATCGACAAG GAGAACGTGG AGCTCTCGCC CACCACTGGC
    CACACCAACA GCGGGAGGAT GCGCCACGGC TCCGCCAGCC AGGTGCAAAA GCAGCGAAGT
    GCTGGCAGCT TGAAACGTCA CAGCATTAAG AAGATTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_415914.2
    CDS236..8737
    Translation

    Target ORF information:

    RefSeq Version XM_415914.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofibromin 1 (NF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_415914.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGAGTCTGAC ATTTAAAGAG AAAATGACAA GCCTTAAATT TAAAGAAAAG
    CCTACTGACT TGGAAACCAA AAGCTACAAG TTCCTGCTGT TATCCTTGGT AAAACTGATC
    CATGCTGATC CAAAGCTTTT GCTGTGTAAT CCAAGGAAGC AAGGGCCTGA GACACAAGGC
    AGCACTGCTG AATTAATTAC AGGCCTTGTA CAACTCGTTC CACAATCCAG CATGCCAGAT
    ATAGCACAAG AAGCCATGGA GGCCTTGCTA GTTCTTCACC AGTTAGACAG CATTGACTTA
    TGGAATCCTG ATGCACCTAT AGAAACTTTC TGGGAAATTA GTTCACAAAT GCTTTTTTAT
    ATCTGCAAGA AATTAACCAG TCATCAGATG CTCAGCAGTA CAGAAATCCT CAAGTGGCTG
    CGAGAGATTC TCATCTGTAG GAATAAATTT CTTCTAAAAA ATAAACAATC AGACAGAACT
    TCCTGTCACT TTCTCTTCCT TTATGATGTC TCTGGAGGTG GAGCTAGTCA GATGTCCCTT
    GACCACGAAG AGATGATGCG CTGTGCTCCC GGAGCCACCC TCAGAAAAGG AAAAGGGAAT
    TCTTCCATGG AAAGTACAGC TGGATGCAGT GGAACACCCA TTTGTCGCCA GGCCCAAACA
    AAATTGGAAG TTGCCTTATA CATGTTTTTG TGGAGTCCGG ATATTGAGGC AGTCCTTGTT
    GCAATGTCCT GTTTCCGTCA CCTCTGTGAG GAAGCAGATA TCAGATGTGG AGTAGATGAA
    GTTTCAGTTC ATAATTTTTT GCCAAACTAC AACACTTTCA TGGAGTTTGC ATCTGTTAGC
    AATATGATGT CAACAGGGAG AGCAGCTCTT CAGAAAAGAG TGATGGCTTT ATTAAGACGT
    ATTGAACATC CAACTGCAGG GAACACAGAG GCTTGGGAGG ATACACATGC AAAATGGGAA
    CAAGCTACAA AACTAATCCT TAACTATCCA AAGACCAAAA TGGAAGACGG GCAGGTTACT
    GAAAGTCTTC ATAAGACAAT TGTTAAGAGA CGAATGTCAC ATGTTAGTGG GGGAGGCTCC
    ATAGACTTGT CTGACACAGA TTCTCTTCAG GAATGGATCA ACATGACGGG ATTCCTCTGT
    GCCCTCGGAG GAGTGTGCCT GCAGCATCGT AGCAATGCAG GATTAGCAAC CTACAGTCCA
    CCCATGGGCC CAGTCAATGA GCGTAAGGGC TCCATGATAT CTATGGTATC CACTGAGGGA
    AATGCAGAGA CTCCTGTTAG CAAATTCATA GATCGATTAC TGACTCTGAT GGTCTGTAAC
    CATGAGAAAG TGGGACTACA AATACGGACT AATATTAAAG ATCTGGTGGG TTTGGAGTTG
    AGTCCAGCAC TTTATCCAAT GCTATTTAAC AAATTGAAGA ATACCATCAG CAAGTTTTTT
    GATTCTCAAG GACAGGTTTT GTTAACTGAG ACCAACACAC AGTTTGTAGA ACAAACCATT
    GCTATAATGA AGAACTTGCT TGACAATCAT ACAGAAGGGA GCTCAGAACA TCTTGGACAA
    GCTAGTATTG AGACAATGAT GCTAAACCTG GTTAGGTATG TGCGTGTACT TGGAAATTTG
    GTACATGCAA TTCAAATAAA AACTAAACTC TGTCAGTTGG TGGAAGTAAT GATGGAGCGG
    AGAGATGACC TTTCATTTTG CCAAGAAATG AAATTTAGGA ACAAAATGGT GGAATATCTG
    ACAGACTGGG TTATGGGAAC ATCTAATCAA GCAACAGATG AAGATGTGAA GTGCTTGACA
    AGAGATTTGG ACCAAGCAAG TATGGAAGCA GTGGTCTCTC TTCTTGCTGG GCTTCCGTTA
    CAGCCTGAAG AAGGAGATGG TGTTGAGTTG ATGGAAGCCA AATCTCAGTT ATTTCTTAAA
    TATTTCACAC TGTTTATGAA CCTTCTAAAT GACTGTAGTG AAGTTGAAGA TGAAAGTGCA
    CAAACAGGTG GCAGGAAACG TGGGATGTCC CGAAGACTGG CTTCGCTGCG GCACTGCACT
    GTTCTTGCTA TGTCAAATTT ACTAAATGCA AATGTGGACA GTGGTCTTAT GCATTCAATA
    GGCTTGGGCT ATCATAAAGA CCTCCAGACA AGAGCTACAT TTATGGAAGT CCTCACCAAA
    ATTCTACAGC AGGGAACAGA ATTTGATACG TTAGCAGAAA CGGTGCTGGC AGATCGGTTT
    GAGAGGCTGG TGGAGCTAGT TACAATGATG GGTGATCAGG GGGAGCTTCC TATTGCTATG
    GCTTTAGCAA ATGTGGTGCC TTGTTCTCAG TGGGATGAGC TAGCTCGTGT TCTGGTCACA
    CTCTTTGATT CCCGGCACTT GCTCTACCAG CTACTTTGGA ATATGTTTTC AAAGGAAGTT
    GAACTGGCAG ATTCCATGCA GACACTCTTC CGAGGAAACA GCTTAGCTAG TAAAATAATG
    ACTTTCTGTT TTAAGGTATA TGGTGCTACT TATTTGCAGA AGCTTTTGGA ACCTTTATTA
    AGAACAGTGA TCACATCCTC TGAATGGCAG CATGTTAGCT TTGAGGTGGA TCCTACAAGA
    CTGGAGCCTA CAGAAAGCCT TGAAGAGAAC CAGCGGAGTC TCTTGCAAAT GACAGAAAAA
    TTTTTTCATG CAATCATTAA TTCTTCTTCG GAATTCCCAC CACAACTTCG CAGTGTGTGC
    CATTGTTTGT ACCAGGCAAC TTGCCACTCT CTACTGAATA AAGCTACCGT AAAAGAAAAA
    AAGGAAAACA AAAAATCAGT TGTTAGTCAG CGATTTCCTC AGAACAGCAT TGGAGCAGTT
    GGGAGTGCCA TGTTCCTGCG GTTCATCAAC CCTGCGATCG TCTCCCCGTA CGAGGCAGGG
    ATTTTAGATA AAAAGCCTCC GCCCAGAATT GAGAGGGGAC TGAAACTGAT GTCAAAGATA
    CTTCAGAGTA TTGCCAACCA CGTCCTGTTT ACAAAAGAAG AACATATGAG GCCTTTTAAT
    GACTTCGTAA AAAGCAATTT TGATGCAGCT CGAAGGTTTT TCCTTGATAT TGCATCTGAT
    TGCCCTGCGA GTGACACTGT CAATCACAGC CTGTCTTTCA TCAGTGATGG CAATGTTCTT
    GCTTTGCACC GGTTGTTGTG GAACAATCAG GAGAAAATTG GCCAGTACCT TTCCAGTAAT
    AGGGACCACA AAGCTGTTGG AAGGCGACCT TTTGACAAAA TGGCTACTCT TCTTGCCTAT
    CTGGGTCCAC CAGAGCACAA ACCTGTGGCA GATACACATT GGTCCAGTTT AAACCTCACC
    AGTTCGAAAT TTGAAGAATT TATGACAAGG CATCAGGTAC ATGAAAAAGA AGAGTTCAAA
    GCATTGAAAA CGTTAAATAT TTTCTACCAG GCTGGGACAT CCAAAGCTGG CAATCCTGTT
    TTCTACTACA TTGCTCGGCG TTTCAAGACT GGTCAGATCA ATGGAGATTT ATTGATATAC
    CACGTCCTGC TAACTCTGAA GCCATACTAC GCAAAGCCAT ATGAAATTGT AGTGGACCTC
    ACACATGCTG GCCCCAGTAA TCGCTTTAAA ACAGACTTTC TTTCTAAATG GTTTGTAGTT
    TTTCCAGGCT TTGCTTATGA AAATGTCTCA GCAGTTTTTA TTTATAACTG TAATTCTTGG
    GTCAGAGAAT ACACCAAATA CCACGAAAGA CTCTTGACAG GTTTGAAAGG AAGCAAAAGG
    CTTATTTTCA TAGACTCTCC AGGAAAACTG GCGGAGCACA TTGAACATGA CCAGCAGAAA
    CTTCCAGCAG CTACCTTAGC TTTGGAAGAG GACTTGAAAG TATTTCACAA TGCCCTCAAA
    TTGGCTCACA AAGACACCAA AGTGTCTATT AAAGTTGGAT CAACAGCTGT GCAGGTGACA
    TCAGCAGAAC GAACAAGAGT CCTGGGACAG ACAGTGTTTT TAAATGATAT ATACTACGCC
    TCTGAAATTG AGGAAATATG TCTTGTTGAT GAGAATCAGT TTACATTGAC GATTGCCAAC
    CAGGGAACGC CACTCACCTT CATGCATCAG GAGTGTGAAG CCATTGTGAG GTCCATTATT
    CATATACGGA CACGGTGGGA GCTGTCGCAA CCAGACTCCA TCCCACAGCA CACTAAAATT
    CGCCCTAAGG ATGTTCCCGG AACACTACTG AACATAGCAT TGCTCAACCT GGGAAGCTCA
    GACCCGAGTT TGCGGTCTGC TGCCTATAAT CTTCTGTGTG CCTTAACCTG TACCTTTAAT
    TTAAAGATTG AGGGCCAGTT ACTGGAAACT TCAGGTCTGT GTATTCCTGC CAACAACACG
    CTCTTTATTG TGTCTATTAG TAAGACTCTT GCAGCTAATG AGCCACACCT TACCCTAGAG
    TTCTTGGAAG AGTGTATTTC AGGATTTAGC AAATCTAGTA TTGAACTGAA ACATCTTTGT
    CTGGAATATA TGACTCCCTG GCTGTCCAAT CTGGTCCGAT TCTGCAAACA CAACGACGAT
    GCGAAGCGCC AGCGAGTCAC TGCCATTCTT GATAAGCTTA TAACAATGAC AATAAATGAA
    AAACAGATGT ACCCTTCTAT TCAGGCTAAA ATATGGGGAA GTCTTGGACA GATTACAGAT
    CTCCTTGACG TAGTGCTGGA CAGTTTCATC AAAACTAGTG CCACGGGGGG CTTAGGATCC
    ATTAAAGCTG AGGTTATGGC AGACACAGCA GTAGCGCTTG CTTCTGGAAA TGTAAAATTG
    GTTTCAAGCA AAGTGATTGG AAGGATGTGC AAAATAATTG ACAAGACTTG TCTGTCTCCA
    ACTCCTACAC TGGAACAGCA CCTGATGTGG GATGATATTG CCATTCTAGC ACGTTACATG
    CTGATGCTCT CCTTCAACAA TTCTCTGGAT GTGGCTGCAC ATCTCCCCTA CCTCTTTCAT
    GTGGTTACTT TGTTGGTGGC CACTGGACCC CTTTCTCTCA GAGCTTCCAC TCATGGTCTT
    GTCATCAACA TCATTCATTC TCTGTGCACT TGCTCACAGC TTCATTTTAG TGAGGAGACC
    AAGCAAGTTT TAAGACTGAG CTTGACTGAA TTTTCTCTGC CTAAATTTTA TTTGCTGTTT
    GGAATCAGCA AAGTGAAATC AGCTGCAGTC ATAGCATTCC GCTCCAGCTA TCGAGACAGG
    TCATTTTCAC CTGGCTCCTA CGAGAGGGAG ACTTTTGCAC TGACCTCCTT GGAAACTGTT
    ACTGAAGCTT TGCTGGAGAT CATGGAGGCT TGTATGAGAG ATATTCCAAC ATGCAAGTGG
    CTGGACCAGT GGACCGAACT AGCACAAAAG TTTGCATTCC AGTACAATCC ATCCCTGCAG
    CCAAGAGCAC TCGTTGTCTT TGGCTGTATA AGTAAGCGTG TCTCTCATGG ACAAATAAAA
    CAAATAATCC GAATTCTTAG CAAGGGGCTT GAGAGCTGTT TAAAAGGCCC TGATAATTAC
    AATAGCCAAG TTCTAATAGA AGCTACGGTT ATAGCACTCA CCAAACTGCA GCCTCTTCTT
    AACAAGGACT CCCCCATGCA CAAGGCCCTC TTCTGGGTAG CCATGGCAGT ACTGCAGTTG
    GATGAAGTCA ACCTATACTC AGCAGGTACA GCACTGCTTG AACAGAATCT GCATACCTTG
    GATAGCCTTC GAGTGTTCAA TGATAAGAGC CCAGAAGAGG TGTTCATGGA GATCAGGAGA
    CCACTTGAAT GGCACTGCAA ACAGATGGAT CATTTTGTTG GGCTAAATTT CAACTCCAAC
    TTCAACTTTG CACTAGTGGG GCACCTCCTG AAAGGGTACA GGCATCCTTC ACCTACCACT
    GTAGCAAGAA CAGTAAGGAT TCTACATACA CTACTAGCTC TGGTTAACAA ACACAGGAAC
    TGTGACAAGT TTGAGGTGAA CACCCAGAGC GTGGCTTACC TAGCAGCTTT GCTGACAGTG
    TCTGAGGAAG TTCGAAGTCG CTGCAGCCTA AAACATAGGA AGTCTCTCTT GCTGACAGAT
    GTTGCAATGG AAAATGTTCC TATGGATACA TATCCCATAC ATCACAGTGA CACTACCTAT
    AGGACACTAA AGGAAAATCA ACCATGGTCA TCACCAAAGG GATCAGACAG ACACCTGGCT
    GCCAGTTACC CGACAGTGGG ACAGATAAGC CCTCGAACAC GAAAGTCCAT GAGTTTGGAT
    ATGGGGCAGC CTTCACAAGC GAACACTAAA AAGCTTCTAG GTACAAGGAA AAGTTTTGAC
    CATTTGATAT CGGACACAAA GGCTCCTAAA AGGCAAGACA TGGAATCTGG AATCACAACG
    CCTCCCAAAA TGAGGAGAGT AGCCGAAAAT GATTATGAAA TGGAAACCCA GAGAATATCA
    CCGCAGCAGC ACCCCCATCT CCGGAAAGTT TCTGTTTCTG AGTCAAATGT CCTCCTGGAT
    GAAGAAGTTC TGACTGATCC AAAAATTCAA GCACTGCTGC TTACAGTTCT TGCCACGTTA
    GTGAAGTACA CTACTGATGA ATTTGACCAG CGGATTCTGT ATGAGTATTT AGCAGAAGCC
    AGTGTTGTCT TCCCAAAGGT CTTTCCTGTT GTGCATAATT TATTGGATTC CAAGATCAAT
    ACCCTCTTGT CGCTGTGTCA AGATCCAAAT TTGTTGAATC CAATTCATGG GATTGTTCAG
    AGTGTTGTCT ACCATGAGGA GTCTCCACCC CAATACCAAA CTTCTTATCT ACAAAGTTTT
    GGATTTAATG GGTTGTGGAG GTTTGCTGGC CCATTTTCCA AGCAAACACA AATCCCAGAC
    TATGCTGAGC TCATAGTGAA GTTTCTTGAT GCCTTGATTG ACACGTATTT GCCTGGAATT
    GATGAGGAAA CCAGTGAAGA ATCTCTCCTG ACACCCACGT CTCCATATCC TCCCGCAGTG
    CAGAGCCAGC TTAGCATTAC AGCTAACCTT AACCTTTCCA ATTCCATGAC CTCGCTCGCA
    ACTTCCCAGC ACTCCCCAGG GATCGACAAG GAGAACGTGG AGCTCTCGCC CACCACTGGC
    CACACCAACA GCGGGAGGAT GCGCCACGGC TCCGCCAGCC AGGTGCAAAA GCAGCGAAGT
    GCTGGCAGCT TGAAACGTCA CAGCATTAAG AAGATTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17417
    Clone ID Related Accession (Same CDS sequence) XM_025142090.1
    Accession Version XM_025142090.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofibromin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
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    5401
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    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGATGCCAAC CAGTCACTGT TTTGCATGGC AGTGGGCCTC TGTTGCTAGT
    CTGACATTTA AAGAGAAAAT GACAAGCCTT AAATTTAAAG AAAAGCCTAC TGACTTGGAA
    ACCAAAAGCT ACAAGTTCCT GCTGTTATCC TTGGTAAAAC TGATCCATGC TGATCCAAAG
    CTTTTGCTGT GTAATCCAAG GAAGCAAGGG CCTGAGACAC AAGGCAGCAC TGCTGAATTA
    ATTACAGGCC TTGTACAACT CGTTCCACAA TCCAGCATGC CAGATATAGC ACAAGAAGCC
    ATGGAGGCCT TGCTAGTTCT TCACCAGTTA GACAGCATTG ACTTATGGAA TCCTGATGCA
    CCTATAGAAA CTTTCTGGGA AATTAGTTCA CAAATGCTTT TTTATATCTG CAAGAAATTA
    ACCAGTCATC AGATGCTCAG CAGTACAGAA ATCCTCAAGT GGCTGCGAGA GATTCTCATC
    TGTAGGAATA AATTTCTTCT AAAAAATAAA CAATCAGACA GAACTTCCTG TCACTTTCTC
    TTCCTTTATG ATGTCTCTGG AGGTGGAGCT AGTCAGATGT CCCTTGACCA CGAAGAGATG
    ATGCGCTGTG CTCCCGGAGC CACCCTCAGA AAAGGAAAAG GGAATTCTTC CATGGAAAGT
    ACAGCTGGAT GCAGTGGAAC ACCCATTTGT CGCCAGGCCC AAACAAAATT GGAAGTTGCC
    TTATACATGT TTTTGTGGAG TCCGGATATT GAGGCAGTCC TTGTTGCAAT GTCCTGTTTC
    CGTCACCTCT GTGAGGAAGC AGATATCAGA TGTGGAGTAG ATGAAGTTTC AGTTCATAAT
    TTTTTGCCAA ACTACAACAC TTTCATGGAG TTTGCATCTG TTAGCAATAT GATGTCAACA
    GGGAGAGCAG CTCTTCAGAA AAGAGTGATG GCTTTATTAA GACGTATTGA ACATCCAACT
    GCAGGGAACA CAGAGGCTTG GGAGGATACA CATGCAAAAT GGGAACAAGC TACAAAACTA
    ATCCTTAACT ATCCAAAGAC CAAAATGGAA GACGGGCAGG TTACTGAAAG TCTTCATAAG
    ACAATTGTTA AGAGACGAAT GTCACATGTT AGTGGGGGAG GCTCCATAGA CTTGTCTGAC
    ACAGATTCTC TTCAGGAATG GATCAACATG ACGGGATTCC TCTGTGCCCT CGGAGGAGTG
    TGCCTGCAGC ATCGTAGCAA TGCAGGATTA GCAACCTACA GTCCACCCAT GGGCCCAGTC
    AATGAGCGTA AGGGCTCCAT GATATCTATG GTATCCACTG AGGGAAATGC AGAGACTCCT
    GTTAGCAAAT TCATAGATCG ATTACTGACT CTGATGGTCT GTAACCATGA GAAAGTGGGA
    CTACAAATAC GGACTAATAT TAAAGATCTG GTGGGTTTGG AGTTGAGTCC AGCACTTTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGATTC TCAAGGACAG
    GTTTTGTTAA CTGAGACCAA CACACAGTTT GTAGAACAAA CCATTGCTAT AATGAAGAAC
    TTGCTTGACA ATCATACAGA AGGGAGCTCA GAACATCTTG GACAAGCTAG TATTGAGACA
    ATGATGCTAA ACCTGGTTAG GTATGTGCGT GTACTTGGAA ATTTGGTACA TGCAATTCAA
    ATAAAAACTA AACTCTGTCA GTTGGTGGAA GTAATGATGG AGCGGAGAGA TGACCTTTCA
    TTTTGCCAAG AAATGAAATT TAGGAACAAA ATGGTGGAAT ATCTGACAGA CTGGGTTATG
    GGAACATCTA ATCAAGCAAC AGATGAAGAT GTGAAGTGCT TGACAAGAGA TTTGGACCAA
    GCAAGTATGG AAGCAGTGGT CTCTCTTCTT GCTGGGCTTC CGTTACAGCC TGAAGAAGGA
    GATGGTGTTG AGTTGATGGA AGCCAAATCT CAGTTATTTC TTAAATATTT CACACTGTTT
    ATGAACCTTC TAAATGACTG TAGTGAAGTT GAAGATGAAA GTGCACAAAC AGGTGGCAGG
    AAACGTGGGA TGTCCCGAAG ACTGGCTTCG CTGCGGCACT GCACTGTTCT TGCTATGTCA
    AATTTACTAA ATGCAAATGT GGACAGTGGT CTTATGCATT CAATAGGCTT GGGCTATCAT
    AAAGACCTCC AGACAAGAGC TACATTTATG GAAGTCCTCA CCAAAATTCT ACAGCAGGGA
    ACAGAATTTG ATACGTTAGC AGAAACGGTG CTGGCAGATC GGTTTGAGAG GCTGGTGGAG
    CTAGTTACAA TGATGGGTGA TCAGGGGGAG CTTCCTATTG CTATGGCTTT AGCAAATGTG
    GTGCCTTGTT CTCAGTGGGA TGAGCTAGCT CGTGTTCTGG TCACACTCTT TGATTCCCGG
    CACTTGCTCT ACCAGCTACT TTGGAATATG TTTTCAAAGG AAGTTGAACT GGCAGATTCC
    ATGCAGACAC TCTTCCGAGG AAACAGCTTA GCTAGTAAAA TAATGACTTT CTGTTTTAAG
    GTATATGGTG CTACTTATTT GCAGAAGCTT TTGGAACCTT TATTAAGAAC AGTGATCACA
    TCCTCTGAAT GGCAGCATGT TAGCTTTGAG GTGGATCCTA CAAGACTGGA GCCTACAGAA
    AGCCTTGAAG AGAACCAGCG GAGTCTCTTG CAAATGACAG AAAAATTTTT TCATGCAATC
    ATTAATTCTT CTTCGGAATT CCCACCACAA CTTCGCAGTG TGTGCCATTG TTTGTACCAG
    GTTGTTAGTC AGCGATTTCC TCAGAACAGC ATTGGAGCAG TTGGGAGTGC CATGTTCCTG
    CGGTTCATCA ACCCTGCGAT CGTCTCCCCG TACGAGGCAG GGATTTTAGA TAAAAAGCCT
    CCGCCCAGAA TTGAGAGGGG ACTGAAACTG ATGTCAAAGA TACTTCAGAG TATTGCCAAC
    CACGTCCTGT TTACAAAAGA AGAACATATG AGGCCTTTTA ATGACTTCGT AAAAAGCAAT
    TTTGATGCAG CTCGAAGGTT TTTCCTTGAT ATTGCATCTG ATTGCCCTGC GAGTGACACT
    GTCAATCACA GCCTGTCTTT CATCAGTGAT GGCAATGTTC TTGCTTTGCA CCGGTTGTTG
    TGGAACAATC AGGAGAAAAT TGGCCAGTAC CTTTCCAGTA ATAGGGACCA CAAAGCTGTT
    GGAAGGCGAC CTTTTGACAA AATGGCTACT CTTCTTGCCT ATCTGGGTCC ACCAGAGCAC
    AAACCTGTGG CAGATACACA TTGGTCCAGT TTAAACCTCA CCAGTTCGAA ATTTGAAGAA
    TTTATGACAA GGCATCAGGT ACATGAAAAA GAAGAGTTCA AAGCATTGAA AACGTTAAAT
    ATTTTCTACC AGGCTGGGAC ATCCAAAGCT GGCAATCCTG TTTTCTACTA CATTGCTCGG
    CGTTTCAAGA CTGGTCAGAT CAATGGAGAT TTATTGATAT ACCACGTCCT GCTAACTCTG
    AAGCCATACT ACGCAAAGCC ATATGAAATT GTAGTGGACC TCACACATGC TGGCCCCAGT
    AATCGCTTTA AAACAGACTT TCTTTCTAAA TGGTTTGTAG TTTTTCCAGG CTTTGCTTAT
    GAAAATGTCT CAGCAGTTTT TATTTATAAC TGTAATTCTT GGGTCAGAGA ATACACCAAA
    TACCACGAAA GACTCTTGAC AGGTTTGAAA GGAAGCAAAA GGCTTATTTT CATAGACTCT
    CCAGGAAAAC TGGCGGAGCA CATTGAACAT GACCAGCAGA AACTTCCAGC AGCTACCTTA
    GCTTTGGAAG AGGACTTGAA AGTATTTCAC AATGCCCTCA AATTGGCTCA CAAAGACACC
    AAAGTGTCTA TTAAAGTTGG ATCAACAGCT GTGCAGGTGA CATCAGCAGA ACGAACAAGA
    GTCCTGGGAC AGACAGTGTT TTTAAATGAT ATATACTACG CCTCTGAAAT TGAGGAAATA
    TGTCTTGTTG ATGAGAATCA GTTTACATTG ACGATTGCCA ACCAGGGAAC GCCACTCACC
    TTCATGCATC AGGAGTGTGA AGCCATTGTG AGGTCCATTA TTCATATACG GACACGGTGG
    GAGCTGTCGC AACCAGACTC CATCCCACAG CACACTAAAA TTCGCCCTAA GGATGTTCCC
    GGAACACTAC TGAACATAGC ATTGCTCAAC CTGGGAAGCT CAGACCCGAG TTTGCGGTCT
    GCTGCCTATA ATCTTCTGTG TGCCTTAACC TGTACCTTTA ATTTAAAGAT TGAGGGCCAG
    TTACTGGAAA CTTCAGGTCT GTGTATTCCT GCCAACAACA CGCTCTTTAT TGTGTCTATT
    AGTAAGACTC TTGCAGCTAA TGAGCCACAC CTTACCCTAG AGTTCTTGGA AGAGTGTATT
    TCAGGATTTA GCAAATCTAG TATTGAACTG AAACATCTTT GTCTGGAATA TATGACTCCC
    TGGCTGTCCA ATCTGGTCCG ATTCTGCAAA CACAACGACG ATGCGAAGCG CCAGCGAGTC
    ACTGCCATTC TTGATAAGCT TATAACAATG ACAATAAATG AAAAACAGAT GTACCCTTCT
    ATTCAGGCTA AAATATGGGG AAGTCTTGGA CAGATTACAG ATCTCCTTGA CGTAGTGCTG
    GACAGTTTCA TCAAAACTAG TGCCACGGGG GGCTTAGGAT CCATTAAAGC TGAGGTTATG
    GCAGACACAG CAGTAGCGCT TGCTTCTGGA AATGTAAAAT TGGTTTCAAG CAAAGTGATT
    GGAAGGATGT GCAAAATAAT TGACAAGACT TGTCTGTCTC CAACTCCTAC ACTGGAACAG
    CACCTGATGT GGGATGATAT TGCCATTCTA GCACGTTACA TGCTGATGCT CTCCTTCAAC
    AATTCTCTGG ATGTGGCTGC ACATCTCCCC TACCTCTTTC ATGTGGTTAC TTTGTTGGTG
    GCCACTGGAC CCCTTTCTCT CAGAGCTTCC ACTCATGGTC TTGTCATCAA CATCATTCAT
    TCTCTGTGCA CTTGCTCACA GCTTCATTTT AGTGAGGAGA CCAAGCAAGT TTTAAGACTG
    AGCTTGACTG AATTTTCTCT GCCTAAATTT TATTTGCTGT TTGGAATCAG CAAAGTGAAA
    TCAGCTGCAG TCATAGCATT CCGCTCCAGC TATCGAGACA GGTCATTTTC ACCTGGCTCC
    TACGAGAGGG AGACTTTTGC ACTGACCTCC TTGGAAACTG TTACTGAAGC TTTGCTGGAG
    ATCATGGAGG CTTGTATGAG AGATATTCCA ACATGCAAGT GGCTGGACCA GTGGACCGAA
    CTAGCACAAA AGTTTGCATT CCAGTACAAT CCATCCCTGC AGCCAAGAGC ACTCGTTGTC
    TTTGGCTGTA TAAGTAAGCG TGTCTCTCAT GGACAAATAA AACAAATAAT CCGAATTCTT
    AGCAAGGGGC TTGAGAGCTG TTTAAAAGGC CCTGATAATT ACAATAGCCA AGTTCTAATA
    GAAGCTACGG TTATAGCACT CACCAAACTG CAGCCTCTTC TTAACAAGGA CTCCCCCATG
    CACAAGGCCC TCTTCTGGGT AGCCATGGCA GTACTGCAGT TGGATGAAGT CAACCTATAC
    TCAGCAGGTA CAGCACTGCT TGAACAGAAT CTGCATACCT TGGATAGCCT TCGAGTGTTC
    AATGATAAGA GCCCAGAAGA GGTGTTCATG GAGATCAGGA GACCACTTGA ATGGCACTGC
    AAACAGATGG ATCATTTTGT TGGGCTAAAT TTCAACTCCA ACTTCAACTT TGCACTAGTG
    GGGCACCTCC TGAAAGGGTA CAGGCATCCT TCACCTACCA CTGTAGCAAG AACAGTAAGG
    ATTCTACATA CACTACTAGC TCTGGTTAAC AAACACAGGA ACTGTGACAA GTTTGAGGTG
    AACACCCAGA GCGTGGCTTA CCTAGCAGCT TTGCTGACAG TGTCTGAGGA AGTTCGAAGT
    CGCTGCAGCC TAAAACATAG GAAGTCTCTC TTGCTGACAG ATGTTGCAAT GGAAAATGTT
    CCTATGGATA CATATCCCAT ACATCACAGT GACACTACCT ATAGGACACT AAAGGAAAAT
    CAACCATGGT CATCACCAAA GGGATCAGAC AGACACCTGG CTGCCAGTTA CCCGACAGTG
    GGACAGATAA GCCCTCGAAC ACGAAAGTCC ATGAGTTTGG ATATGGGGCA GCCTTCACAA
    GCGAACACTA AAAAGCTTCT AGGTACAAGG AAAAGTTTTG ACCATTTGAT ATCGGACACA
    AAGGCTCCTA AAAGGCAAGA CATGGAATCT GGAATCACAA CGCCTCCCAA AATGAGGAGA
    GTAGCCGAAA ATGATTATGA AATGGAAACC CAGAGAATAT CACCGCAGCA GCACCCCCAT
    CTCCGGAAAG TTTCTGTTTC TGAGTCAAAT GTCCTCCTGG ATGAAGAAGT TCTGACTGAT
    CCAAAAATTC AAGCACTGCT GCTTACAGTT CTTGCCACGT TAGTGAAGTA CACTACTGAT
    GAATTTGACC AGCGGATTCT GTATGAGTAT TTAGCAGAAG CCAGTGTTGT CTTCCCAAAG
    GTCTTTCCTG TTGTGCATAA TTTATTGGAT TCCAAGATCA ATACCCTCTT GTCGCTGTGT
    CAAGATCCAA ATTTGTTGAA TCCAATTCAT GGGATTGTTC AGAGTGTTGT CTACCATGAG
    GAGTCTCCAC CCCAATACCA AACTTCTTAT CTACAAAGTT TTGGATTTAA TGGGTTGTGG
    AGGTTTGCTG GCCCATTTTC CAAGCAAACA CAAATCCCAG ACTATGCTGA GCTCATAGTG
    AAGTTTCTTG ATGCCTTGAT TGACACGTAT TTGCCTGGAA TTGATGAGGA AACCAGTGAA
    GAATCTCTCC TGACACCCAC GTCTCCATAT CCTCCCGCAG TGCAGAGCCA GCTTAGCATT
    ACAGCTAACC TTAACCTTTC CAATTCCATG ACCTCGCTCG CAACTTCCCA GCACTCCCCA
    GGGATCGACA AGGAGAACGT GGAGCTCTCG CCCACCACTG GCCACACCAA CAGCGGGAGG
    ATGCGCCACG GCTCCGCCAG CCAGGTGCAA AAGCAGCGAA GTGCTGGCAG CTTGAAACGT
    CACAGCATTA AGAAGATTGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024997858.1
    CDS237..8720
    Translation

    Target ORF information:

    RefSeq Version XM_025142090.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofibromin 1 (NF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025142090.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGATGCCAAC CAGTCACTGT TTTGCATGGC AGTGGGCCTC TGTTGCTAGT
    CTGACATTTA AAGAGAAAAT GACAAGCCTT AAATTTAAAG AAAAGCCTAC TGACTTGGAA
    ACCAAAAGCT ACAAGTTCCT GCTGTTATCC TTGGTAAAAC TGATCCATGC TGATCCAAAG
    CTTTTGCTGT GTAATCCAAG GAAGCAAGGG CCTGAGACAC AAGGCAGCAC TGCTGAATTA
    ATTACAGGCC TTGTACAACT CGTTCCACAA TCCAGCATGC CAGATATAGC ACAAGAAGCC
    ATGGAGGCCT TGCTAGTTCT TCACCAGTTA GACAGCATTG ACTTATGGAA TCCTGATGCA
    CCTATAGAAA CTTTCTGGGA AATTAGTTCA CAAATGCTTT TTTATATCTG CAAGAAATTA
    ACCAGTCATC AGATGCTCAG CAGTACAGAA ATCCTCAAGT GGCTGCGAGA GATTCTCATC
    TGTAGGAATA AATTTCTTCT AAAAAATAAA CAATCAGACA GAACTTCCTG TCACTTTCTC
    TTCCTTTATG ATGTCTCTGG AGGTGGAGCT AGTCAGATGT CCCTTGACCA CGAAGAGATG
    ATGCGCTGTG CTCCCGGAGC CACCCTCAGA AAAGGAAAAG GGAATTCTTC CATGGAAAGT
    ACAGCTGGAT GCAGTGGAAC ACCCATTTGT CGCCAGGCCC AAACAAAATT GGAAGTTGCC
    TTATACATGT TTTTGTGGAG TCCGGATATT GAGGCAGTCC TTGTTGCAAT GTCCTGTTTC
    CGTCACCTCT GTGAGGAAGC AGATATCAGA TGTGGAGTAG ATGAAGTTTC AGTTCATAAT
    TTTTTGCCAA ACTACAACAC TTTCATGGAG TTTGCATCTG TTAGCAATAT GATGTCAACA
    GGGAGAGCAG CTCTTCAGAA AAGAGTGATG GCTTTATTAA GACGTATTGA ACATCCAACT
    GCAGGGAACA CAGAGGCTTG GGAGGATACA CATGCAAAAT GGGAACAAGC TACAAAACTA
    ATCCTTAACT ATCCAAAGAC CAAAATGGAA GACGGGCAGG TTACTGAAAG TCTTCATAAG
    ACAATTGTTA AGAGACGAAT GTCACATGTT AGTGGGGGAG GCTCCATAGA CTTGTCTGAC
    ACAGATTCTC TTCAGGAATG GATCAACATG ACGGGATTCC TCTGTGCCCT CGGAGGAGTG
    TGCCTGCAGC ATCGTAGCAA TGCAGGATTA GCAACCTACA GTCCACCCAT GGGCCCAGTC
    AATGAGCGTA AGGGCTCCAT GATATCTATG GTATCCACTG AGGGAAATGC AGAGACTCCT
    GTTAGCAAAT TCATAGATCG ATTACTGACT CTGATGGTCT GTAACCATGA GAAAGTGGGA
    CTACAAATAC GGACTAATAT TAAAGATCTG GTGGGTTTGG AGTTGAGTCC AGCACTTTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGATTC TCAAGGACAG
    GTTTTGTTAA CTGAGACCAA CACACAGTTT GTAGAACAAA CCATTGCTAT AATGAAGAAC
    TTGCTTGACA ATCATACAGA AGGGAGCTCA GAACATCTTG GACAAGCTAG TATTGAGACA
    ATGATGCTAA ACCTGGTTAG GTATGTGCGT GTACTTGGAA ATTTGGTACA TGCAATTCAA
    ATAAAAACTA AACTCTGTCA GTTGGTGGAA GTAATGATGG AGCGGAGAGA TGACCTTTCA
    TTTTGCCAAG AAATGAAATT TAGGAACAAA ATGGTGGAAT ATCTGACAGA CTGGGTTATG
    GGAACATCTA ATCAAGCAAC AGATGAAGAT GTGAAGTGCT TGACAAGAGA TTTGGACCAA
    GCAAGTATGG AAGCAGTGGT CTCTCTTCTT GCTGGGCTTC CGTTACAGCC TGAAGAAGGA
    GATGGTGTTG AGTTGATGGA AGCCAAATCT CAGTTATTTC TTAAATATTT CACACTGTTT
    ATGAACCTTC TAAATGACTG TAGTGAAGTT GAAGATGAAA GTGCACAAAC AGGTGGCAGG
    AAACGTGGGA TGTCCCGAAG ACTGGCTTCG CTGCGGCACT GCACTGTTCT TGCTATGTCA
    AATTTACTAA ATGCAAATGT GGACAGTGGT CTTATGCATT CAATAGGCTT GGGCTATCAT
    AAAGACCTCC AGACAAGAGC TACATTTATG GAAGTCCTCA CCAAAATTCT ACAGCAGGGA
    ACAGAATTTG ATACGTTAGC AGAAACGGTG CTGGCAGATC GGTTTGAGAG GCTGGTGGAG
    CTAGTTACAA TGATGGGTGA TCAGGGGGAG CTTCCTATTG CTATGGCTTT AGCAAATGTG
    GTGCCTTGTT CTCAGTGGGA TGAGCTAGCT CGTGTTCTGG TCACACTCTT TGATTCCCGG
    CACTTGCTCT ACCAGCTACT TTGGAATATG TTTTCAAAGG AAGTTGAACT GGCAGATTCC
    ATGCAGACAC TCTTCCGAGG AAACAGCTTA GCTAGTAAAA TAATGACTTT CTGTTTTAAG
    GTATATGGTG CTACTTATTT GCAGAAGCTT TTGGAACCTT TATTAAGAAC AGTGATCACA
    TCCTCTGAAT GGCAGCATGT TAGCTTTGAG GTGGATCCTA CAAGACTGGA GCCTACAGAA
    AGCCTTGAAG AGAACCAGCG GAGTCTCTTG CAAATGACAG AAAAATTTTT TCATGCAATC
    ATTAATTCTT CTTCGGAATT CCCACCACAA CTTCGCAGTG TGTGCCATTG TTTGTACCAG
    GTTGTTAGTC AGCGATTTCC TCAGAACAGC ATTGGAGCAG TTGGGAGTGC CATGTTCCTG
    CGGTTCATCA ACCCTGCGAT CGTCTCCCCG TACGAGGCAG GGATTTTAGA TAAAAAGCCT
    CCGCCCAGAA TTGAGAGGGG ACTGAAACTG ATGTCAAAGA TACTTCAGAG TATTGCCAAC
    CACGTCCTGT TTACAAAAGA AGAACATATG AGGCCTTTTA ATGACTTCGT AAAAAGCAAT
    TTTGATGCAG CTCGAAGGTT TTTCCTTGAT ATTGCATCTG ATTGCCCTGC GAGTGACACT
    GTCAATCACA GCCTGTCTTT CATCAGTGAT GGCAATGTTC TTGCTTTGCA CCGGTTGTTG
    TGGAACAATC AGGAGAAAAT TGGCCAGTAC CTTTCCAGTA ATAGGGACCA CAAAGCTGTT
    GGAAGGCGAC CTTTTGACAA AATGGCTACT CTTCTTGCCT ATCTGGGTCC ACCAGAGCAC
    AAACCTGTGG CAGATACACA TTGGTCCAGT TTAAACCTCA CCAGTTCGAA ATTTGAAGAA
    TTTATGACAA GGCATCAGGT ACATGAAAAA GAAGAGTTCA AAGCATTGAA AACGTTAAAT
    ATTTTCTACC AGGCTGGGAC ATCCAAAGCT GGCAATCCTG TTTTCTACTA CATTGCTCGG
    CGTTTCAAGA CTGGTCAGAT CAATGGAGAT TTATTGATAT ACCACGTCCT GCTAACTCTG
    AAGCCATACT ACGCAAAGCC ATATGAAATT GTAGTGGACC TCACACATGC TGGCCCCAGT
    AATCGCTTTA AAACAGACTT TCTTTCTAAA TGGTTTGTAG TTTTTCCAGG CTTTGCTTAT
    GAAAATGTCT CAGCAGTTTT TATTTATAAC TGTAATTCTT GGGTCAGAGA ATACACCAAA
    TACCACGAAA GACTCTTGAC AGGTTTGAAA GGAAGCAAAA GGCTTATTTT CATAGACTCT
    CCAGGAAAAC TGGCGGAGCA CATTGAACAT GACCAGCAGA AACTTCCAGC AGCTACCTTA
    GCTTTGGAAG AGGACTTGAA AGTATTTCAC AATGCCCTCA AATTGGCTCA CAAAGACACC
    AAAGTGTCTA TTAAAGTTGG ATCAACAGCT GTGCAGGTGA CATCAGCAGA ACGAACAAGA
    GTCCTGGGAC AGACAGTGTT TTTAAATGAT ATATACTACG CCTCTGAAAT TGAGGAAATA
    TGTCTTGTTG ATGAGAATCA GTTTACATTG ACGATTGCCA ACCAGGGAAC GCCACTCACC
    TTCATGCATC AGGAGTGTGA AGCCATTGTG AGGTCCATTA TTCATATACG GACACGGTGG
    GAGCTGTCGC AACCAGACTC CATCCCACAG CACACTAAAA TTCGCCCTAA GGATGTTCCC
    GGAACACTAC TGAACATAGC ATTGCTCAAC CTGGGAAGCT CAGACCCGAG TTTGCGGTCT
    GCTGCCTATA ATCTTCTGTG TGCCTTAACC TGTACCTTTA ATTTAAAGAT TGAGGGCCAG
    TTACTGGAAA CTTCAGGTCT GTGTATTCCT GCCAACAACA CGCTCTTTAT TGTGTCTATT
    AGTAAGACTC TTGCAGCTAA TGAGCCACAC CTTACCCTAG AGTTCTTGGA AGAGTGTATT
    TCAGGATTTA GCAAATCTAG TATTGAACTG AAACATCTTT GTCTGGAATA TATGACTCCC
    TGGCTGTCCA ATCTGGTCCG ATTCTGCAAA CACAACGACG ATGCGAAGCG CCAGCGAGTC
    ACTGCCATTC TTGATAAGCT TATAACAATG ACAATAAATG AAAAACAGAT GTACCCTTCT
    ATTCAGGCTA AAATATGGGG AAGTCTTGGA CAGATTACAG ATCTCCTTGA CGTAGTGCTG
    GACAGTTTCA TCAAAACTAG TGCCACGGGG GGCTTAGGAT CCATTAAAGC TGAGGTTATG
    GCAGACACAG CAGTAGCGCT TGCTTCTGGA AATGTAAAAT TGGTTTCAAG CAAAGTGATT
    GGAAGGATGT GCAAAATAAT TGACAAGACT TGTCTGTCTC CAACTCCTAC ACTGGAACAG
    CACCTGATGT GGGATGATAT TGCCATTCTA GCACGTTACA TGCTGATGCT CTCCTTCAAC
    AATTCTCTGG ATGTGGCTGC ACATCTCCCC TACCTCTTTC ATGTGGTTAC TTTGTTGGTG
    GCCACTGGAC CCCTTTCTCT CAGAGCTTCC ACTCATGGTC TTGTCATCAA CATCATTCAT
    TCTCTGTGCA CTTGCTCACA GCTTCATTTT AGTGAGGAGA CCAAGCAAGT TTTAAGACTG
    AGCTTGACTG AATTTTCTCT GCCTAAATTT TATTTGCTGT TTGGAATCAG CAAAGTGAAA
    TCAGCTGCAG TCATAGCATT CCGCTCCAGC TATCGAGACA GGTCATTTTC ACCTGGCTCC
    TACGAGAGGG AGACTTTTGC ACTGACCTCC TTGGAAACTG TTACTGAAGC TTTGCTGGAG
    ATCATGGAGG CTTGTATGAG AGATATTCCA ACATGCAAGT GGCTGGACCA GTGGACCGAA
    CTAGCACAAA AGTTTGCATT CCAGTACAAT CCATCCCTGC AGCCAAGAGC ACTCGTTGTC
    TTTGGCTGTA TAAGTAAGCG TGTCTCTCAT GGACAAATAA AACAAATAAT CCGAATTCTT
    AGCAAGGGGC TTGAGAGCTG TTTAAAAGGC CCTGATAATT ACAATAGCCA AGTTCTAATA
    GAAGCTACGG TTATAGCACT CACCAAACTG CAGCCTCTTC TTAACAAGGA CTCCCCCATG
    CACAAGGCCC TCTTCTGGGT AGCCATGGCA GTACTGCAGT TGGATGAAGT CAACCTATAC
    TCAGCAGGTA CAGCACTGCT TGAACAGAAT CTGCATACCT TGGATAGCCT TCGAGTGTTC
    AATGATAAGA GCCCAGAAGA GGTGTTCATG GAGATCAGGA GACCACTTGA ATGGCACTGC
    AAACAGATGG ATCATTTTGT TGGGCTAAAT TTCAACTCCA ACTTCAACTT TGCACTAGTG
    GGGCACCTCC TGAAAGGGTA CAGGCATCCT TCACCTACCA CTGTAGCAAG AACAGTAAGG
    ATTCTACATA CACTACTAGC TCTGGTTAAC AAACACAGGA ACTGTGACAA GTTTGAGGTG
    AACACCCAGA GCGTGGCTTA CCTAGCAGCT TTGCTGACAG TGTCTGAGGA AGTTCGAAGT
    CGCTGCAGCC TAAAACATAG GAAGTCTCTC TTGCTGACAG ATGTTGCAAT GGAAAATGTT
    CCTATGGATA CATATCCCAT ACATCACAGT GACACTACCT ATAGGACACT AAAGGAAAAT
    CAACCATGGT CATCACCAAA GGGATCAGAC AGACACCTGG CTGCCAGTTA CCCGACAGTG
    GGACAGATAA GCCCTCGAAC ACGAAAGTCC ATGAGTTTGG ATATGGGGCA GCCTTCACAA
    GCGAACACTA AAAAGCTTCT AGGTACAAGG AAAAGTTTTG ACCATTTGAT ATCGGACACA
    AAGGCTCCTA AAAGGCAAGA CATGGAATCT GGAATCACAA CGCCTCCCAA AATGAGGAGA
    GTAGCCGAAA ATGATTATGA AATGGAAACC CAGAGAATAT CACCGCAGCA GCACCCCCAT
    CTCCGGAAAG TTTCTGTTTC TGAGTCAAAT GTCCTCCTGG ATGAAGAAGT TCTGACTGAT
    CCAAAAATTC AAGCACTGCT GCTTACAGTT CTTGCCACGT TAGTGAAGTA CACTACTGAT
    GAATTTGACC AGCGGATTCT GTATGAGTAT TTAGCAGAAG CCAGTGTTGT CTTCCCAAAG
    GTCTTTCCTG TTGTGCATAA TTTATTGGAT TCCAAGATCA ATACCCTCTT GTCGCTGTGT
    CAAGATCCAA ATTTGTTGAA TCCAATTCAT GGGATTGTTC AGAGTGTTGT CTACCATGAG
    GAGTCTCCAC CCCAATACCA AACTTCTTAT CTACAAAGTT TTGGATTTAA TGGGTTGTGG
    AGGTTTGCTG GCCCATTTTC CAAGCAAACA CAAATCCCAG ACTATGCTGA GCTCATAGTG
    AAGTTTCTTG ATGCCTTGAT TGACACGTAT TTGCCTGGAA TTGATGAGGA AACCAGTGAA
    GAATCTCTCC TGACACCCAC GTCTCCATAT CCTCCCGCAG TGCAGAGCCA GCTTAGCATT
    ACAGCTAACC TTAACCTTTC CAATTCCATG ACCTCGCTCG CAACTTCCCA GCACTCCCCA
    GGGATCGACA AGGAGAACGT GGAGCTCTCG CCCACCACTG GCCACACCAA CAGCGGGAGG
    ATGCGCCACG GCTCCGCCAG CCAGGTGCAA AAGCAGCGAA GTGCTGGCAG CTTGAAACGT
    CACAGCATTA AGAAGATTGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17419
    Clone ID Related Accession (Same CDS sequence) XM_003642416.3
    Accession Version XM_003642416.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofibromin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_003642416.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGAGTCTGAC ATTTAAAGAG AAAATGACAA GCCTTAAATT TAAAGAAAAG
    CCTACTGACT TGGAAACCAA AAGCTACAAG TTCCTGCTGT TATCCTTGGT AAAACTGATC
    CATGCTGATC CAAAGCTTTT GCTGTGTAAT CCAAGGAAGC AAGGGCCTGA GACACAAGGC
    AGCACTGCTG AATTAATTAC AGGCCTTGTA CAACTCGTTC CACAATCCAG CATGCCAGAT
    ATAGCACAAG AAGCCATGGA GGCCTTGCTA GTTCTTCACC AGTTAGACAG CATTGACTTA
    TGGAATCCTG ATGCACCTAT AGAAACTTTC TGGGAAATTA GTTCACAAAT GCTTTTTTAT
    ATCTGCAAGA AATTAACCAG TCATCAGATG CTCAGCAGTA CAGAAATCCT CAAGTGGCTG
    CGAGAGATTC TCATCTGTAG GAATAAATTT CTTCTAAAAA ATAAACAATC AGACAGAACT
    TCCTGTCACT TTCTCTTCCT TTATGATGTC TCTGGAGGTG GAGCTAGTCA GATGTCCCTT
    GACCACGAAG AGATGATGCG CTGTGCTCCC GGAGCCACCC TCAGAAAAGG AAAAGGGAAT
    TCTTCCATGG AAAGTACAGC TGGATGCAGT GGAACACCCA TTTGTCGCCA GGCCCAAACA
    AAATTGGAAG TTGCCTTATA CATGTTTTTG TGGAGTCCGG ATATTGAGGC AGTCCTTGTT
    GCAATGTCCT GTTTCCGTCA CCTCTGTGAG GAAGCAGATA TCAGATGTGG AGTAGATGAA
    GTTTCAGTTC ATAATTTTTT GCCAAACTAC AACACTTTCA TGGAGTTTGC ATCTGTTAGC
    AATATGATGT CAACAGGGAG AGCAGCTCTT CAGAAAAGAG TGATGGCTTT ATTAAGACGT
    ATTGAACATC CAACTGCAGG GAACACAGAG GCTTGGGAGG ATACACATGC AAAATGGGAA
    CAAGCTACAA AACTAATCCT TAACTATCCA AAGACCAAAA TGGAAGACGG GCAGGTTACT
    GAAAGTCTTC ATAAGACAAT TGTTAAGAGA CGAATGTCAC ATGTTAGTGG GGGAGGCTCC
    ATAGACTTGT CTGACACAGA TTCTCTTCAG GAATGGATCA ACATGACGGG ATTCCTCTGT
    GCCCTCGGAG GAGTGTGCCT GCAGCATCGT AGCAATGCAG GATTAGCAAC CTACAGTCCA
    CCCATGGGCC CAGTCAATGA GCGTAAGGGC TCCATGATAT CTATGGTATC CACTGAGGGA
    AATGCAGAGA CTCCTGTTAG CAAATTCATA GATCGATTAC TGACTCTGAT GGTCTGTAAC
    CATGAGAAAG TGGGACTACA AATACGGACT AATATTAAAG ATCTGGTGGG TTTGGAGTTG
    AGTCCAGCAC TTTATCCAAT GCTATTTAAC AAATTGAAGA ATACCATCAG CAAGTTTTTT
    GATTCTCAAG GACAGGTTTT GTTAACTGAG ACCAACACAC AGTTTGTAGA ACAAACCATT
    GCTATAATGA AGAACTTGCT TGACAATCAT ACAGAAGGGA GCTCAGAACA TCTTGGACAA
    GCTAGTATTG AGACAATGAT GCTAAACCTG GTTAGGTATG TGCGTGTACT TGGAAATTTG
    GTACATGCAA TTCAAATAAA AACTAAACTC TGTCAGTTGG TGGAAGTAAT GATGGAGCGG
    AGAGATGACC TTTCATTTTG CCAAGAAATG AAATTTAGGA ACAAAATGGT GGAATATCTG
    ACAGACTGGG TTATGGGAAC ATCTAATCAA GCAACAGATG AAGATGTGAA GTGCTTGACA
    AGAGATTTGG ACCAAGCAAG TATGGAAGCA GTGGTCTCTC TTCTTGCTGG GCTTCCGTTA
    CAGCCTGAAG AAGGAGATGG TGTTGAGTTG ATGGAAGCCA AATCTCAGTT ATTTCTTAAA
    TATTTCACAC TGTTTATGAA CCTTCTAAAT GACTGTAGTG AAGTTGAAGA TGAAAGTGCA
    CAAACAGGTG GCAGGAAACG TGGGATGTCC CGAAGACTGG CTTCGCTGCG GCACTGCACT
    GTTCTTGCTA TGTCAAATTT ACTAAATGCA AATGTGGACA GTGGTCTTAT GCATTCAATA
    GGCTTGGGCT ATCATAAAGA CCTCCAGACA AGAGCTACAT TTATGGAAGT CCTCACCAAA
    ATTCTACAGC AGGGAACAGA ATTTGATACG TTAGCAGAAA CGGTGCTGGC AGATCGGTTT
    GAGAGGCTGG TGGAGCTAGT TACAATGATG GGTGATCAGG GGGAGCTTCC TATTGCTATG
    GCTTTAGCAA ATGTGGTGCC TTGTTCTCAG TGGGATGAGC TAGCTCGTGT TCTGGTCACA
    CTCTTTGATT CCCGGCACTT GCTCTACCAG CTACTTTGGA ATATGTTTTC AAAGGAAGTT
    GAACTGGCAG ATTCCATGCA GACACTCTTC CGAGGAAACA GCTTAGCTAG TAAAATAATG
    ACTTTCTGTT TTAAGGTATA TGGTGCTACT TATTTGCAGA AGCTTTTGGA ACCTTTATTA
    AGAACAGTGA TCACATCCTC TGAATGGCAG CATGTTAGCT TTGAGGTGGA TCCTACAAGA
    CTGGAGCCTA CAGAAAGCCT TGAAGAGAAC CAGCGGAGTC TCTTGCAAAT GACAGAAAAA
    TTTTTTCATG CAATCATTAA TTCTTCTTCG GAATTCCCAC CACAACTTCG CAGTGTGTGC
    CATTGTTTGT ACCAGGTTGT TAGTCAGCGA TTTCCTCAGA ACAGCATTGG AGCAGTTGGG
    AGTGCCATGT TCCTGCGGTT CATCAACCCT GCGATCGTCT CCCCGTACGA GGCAGGGATT
    TTAGATAAAA AGCCTCCGCC CAGAATTGAG AGGGGACTGA AACTGATGTC AAAGATACTT
    CAGAGTATTG CCAACCACGT CCTGTTTACA AAAGAAGAAC ATATGAGGCC TTTTAATGAC
    TTCGTAAAAA GCAATTTTGA TGCAGCTCGA AGGTTTTTCC TTGATATTGC ATCTGATTGC
    CCTGCGAGTG ACACTGTCAA TCACAGCCTG TCTTTCATCA GTGATGGCAA TGTTCTTGCT
    TTGCACCGGT TGTTGTGGAA CAATCAGGAG AAAATTGGCC AGTACCTTTC CAGTAATAGG
    GACCACAAAG CTGTTGGAAG GCGACCTTTT GACAAAATGG CTACTCTTCT TGCCTATCTG
    GGTCCACCAG AGCACAAACC TGTGGCAGAT ACACATTGGT CCAGTTTAAA CCTCACCAGT
    TCGAAATTTG AAGAATTTAT GACAAGGCAT CAGGTACATG AAAAAGAAGA GTTCAAAGCA
    TTGAAAACGT TAAATATTTT CTACCAGGCT GGGACATCCA AAGCTGGCAA TCCTGTTTTC
    TACTACATTG CTCGGCGTTT CAAGACTGGT CAGATCAATG GAGATTTATT GATATACCAC
    GTCCTGCTAA CTCTGAAGCC ATACTACGCA AAGCCATATG AAATTGTAGT GGACCTCACA
    CATGCTGGCC CCAGTAATCG CTTTAAAACA GACTTTCTTT CTAAATGGTT TGTAGTTTTT
    CCAGGCTTTG CTTATGAAAA TGTCTCAGCA GTTTTTATTT ATAACTGTAA TTCTTGGGTC
    AGAGAATACA CCAAATACCA CGAAAGACTC TTGACAGGTT TGAAAGGAAG CAAAAGGCTT
    ATTTTCATAG ACTCTCCAGG AAAACTGGCG GAGCACATTG AACATGACCA GCAGAAACTT
    CCAGCAGCTA CCTTAGCTTT GGAAGAGGAC TTGAAAGTAT TTCACAATGC CCTCAAATTG
    GCTCACAAAG ACACCAAAGT GTCTATTAAA GTTGGATCAA CAGCTGTGCA GGTGACATCA
    GCAGAACGAA CAAGAGTCCT GGGACAGACA GTGTTTTTAA ATGATATATA CTACGCCTCT
    GAAATTGAGG AAATATGTCT TGTTGATGAG AATCAGTTTA CATTGACGAT TGCCAACCAG
    GGAACGCCAC TCACCTTCAT GCATCAGGAG TGTGAAGCCA TTGTGAGGTC CATTATTCAT
    ATACGGACAC GGTGGGAGCT GTCGCAACCA GACTCCATCC CACAGCACAC TAAAATTCGC
    CCTAAGGATG TTCCCGGAAC ACTACTGAAC ATAGCATTGC TCAACCTGGG AAGCTCAGAC
    CCGAGTTTGC GGTCTGCTGC CTATAATCTT CTGTGTGCCT TAACCTGTAC CTTTAATTTA
    AAGATTGAGG GCCAGTTACT GGAAACTTCA GGTCTGTGTA TTCCTGCCAA CAACACGCTC
    TTTATTGTGT CTATTAGTAA GACTCTTGCA GCTAATGAGC CACACCTTAC CCTAGAGTTC
    TTGGAAGAGT GTATTTCAGG ATTTAGCAAA TCTAGTATTG AACTGAAACA TCTTTGTCTG
    GAATATATGA CTCCCTGGCT GTCCAATCTG GTCCGATTCT GCAAACACAA CGACGATGCG
    AAGCGCCAGC GAGTCACTGC CATTCTTGAT AAGCTTATAA CAATGACAAT AAATGAAAAA
    CAGATGTACC CTTCTATTCA GGCTAAAATA TGGGGAAGTC TTGGACAGAT TACAGATCTC
    CTTGACGTAG TGCTGGACAG TTTCATCAAA ACTAGTGCCA CGGGGGGCTT AGGATCCATT
    AAAGCTGAGG TTATGGCAGA CACAGCAGTA GCGCTTGCTT CTGGAAATGT AAAATTGGTT
    TCAAGCAAAG TGATTGGAAG GATGTGCAAA ATAATTGACA AGACTTGTCT GTCTCCAACT
    CCTACACTGG AACAGCACCT GATGTGGGAT GATATTGCCA TTCTAGCACG TTACATGCTG
    ATGCTCTCCT TCAACAATTC TCTGGATGTG GCTGCACATC TCCCCTACCT CTTTCATGTG
    GTTACTTTGT TGGTGGCCAC TGGACCCCTT TCTCTCAGAG CTTCCACTCA TGGTCTTGTC
    ATCAACATCA TTCATTCTCT GTGCACTTGC TCACAGCTTC ATTTTAGTGA GGAGACCAAG
    CAAGTTTTAA GACTGAGCTT GACTGAATTT TCTCTGCCTA AATTTTATTT GCTGTTTGGA
    ATCAGCAAAG TGAAATCAGC TGCAGTCATA GCATTCCGCT CCAGCTATCG AGACAGGTCA
    TTTTCACCTG GCTCCTACGA GAGGGAGACT TTTGCACTGA CCTCCTTGGA AACTGTTACT
    GAAGCTTTGC TGGAGATCAT GGAGGCTTGT ATGAGAGATA TTCCAACATG CAAGTGGCTG
    GACCAGTGGA CCGAACTAGC ACAAAAGTTT GCATTCCAGT ACAATCCATC CCTGCAGCCA
    AGAGCACTCG TTGTCTTTGG CTGTATAAGT AAGCGTGTCT CTCATGGACA AATAAAACAA
    ATAATCCGAA TTCTTAGCAA GGGGCTTGAG AGCTGTTTAA AAGGCCCTGA TAATTACAAT
    AGCCAAGTTC TAATAGAAGC TACGGTTATA GCACTCACCA AACTGCAGCC TCTTCTTAAC
    AAGGACTCCC CCATGCACAA GGCCCTCTTC TGGGTAGCCA TGGCAGTACT GCAGTTGGAT
    GAAGTCAACC TATACTCAGC AGGTACAGCA CTGCTTGAAC AGAATCTGCA TACCTTGGAT
    AGCCTTCGAG TGTTCAATGA TAAGAGCCCA GAAGAGGTGT TCATGGAGAT CAGGAGACCA
    CTTGAATGGC ACTGCAAACA GATGGATCAT TTTGTTGGGC TAAATTTCAA CTCCAACTTC
    AACTTTGCAC TAGTGGGGCA CCTCCTGAAA GGGTACAGGC ATCCTTCACC TACCACTGTA
    GCAAGAACAG TAAGGATTCT ACATACACTA CTAGCTCTGG TTAACAAACA CAGGAACTGT
    GACAAGTTTG AGGTGAACAC CCAGAGCGTG GCTTACCTAG CAGCTTTGCT GACAGTGTCT
    GAGGAAGTTC GAAGTCGCTG CAGCCTAAAA CATAGGAAGT CTCTCTTGCT GACAGATGTT
    GCAATGGAAA ATGTTCCTAT GGATACATAT CCCATACATC ACAGTGACAC TACCTATAGG
    ACACTAAAGG AAAATCAACC ATGGTCATCA CCAAAGGGAT CAGACAGACA CCTGGCTGCC
    AGTTACCCGA CAGTGGGACA GATAAGCCCT CGAACACGAA AGTCCATGAG TTTGGATATG
    GGGCAGCCTT CACAAGCGAA CACTAAAAAG CTTCTAGGTA CAAGGAAAAG TTTTGACCAT
    TTGATATCGG ACACAAAGGC TCCTAAAAGG CAAGACATGG AATCTGGAAT CACAACGCCT
    CCCAAAATGA GGAGAGTAGC CGAAAATGAT TATGAAATGG AAACCCAGAG AATATCACCG
    CAGCAGCACC CCCATCTCCG GAAAGTTTCT GTTTCTGAGT CAAATGTCCT CCTGGATGAA
    GAAGTTCTGA CTGATCCAAA AATTCAAGCA CTGCTGCTTA CAGTTCTTGC CACGTTAGTG
    AAGTACACTA CTGATGAATT TGACCAGCGG ATTCTGTATG AGTATTTAGC AGAAGCCAGT
    GTTGTCTTCC CAAAGGTCTT TCCTGTTGTG CATAATTTAT TGGATTCCAA GATCAATACC
    CTCTTGTCGC TGTGTCAAGA TCCAAATTTG TTGAATCCAA TTCATGGGAT TGTTCAGAGT
    GTTGTCTACC ATGAGGAGTC TCCACCCCAA TACCAAACTT CTTATCTACA AAGTTTTGGA
    TTTAATGGGT TGTGGAGGTT TGCTGGCCCA TTTTCCAAGC AAACACAAAT CCCAGACTAT
    GCTGAGCTCA TAGTGAAGTT TCTTGATGCC TTGATTGACA CGTATTTGCC TGGAATTGAT
    GAGGAAACCA GTGAAGAATC TCTCCTGACA CCCACGTCTC CATATCCTCC CGCAGTGCAG
    AGCCAGCTTA GCATTACAGC TAACCTTAAC CTTTCCAATT CCATGACCTC GCTCGCAACT
    TCCCAGCACT CCCCAGGGAT CGACAAGGAG AACGTGGAGC TCTCGCCCAC CACTGGCCAC
    ACCAACAGCG GGAGGATGCG CCACGGCTCC GCCAGCCAGG TGCAAAAGCA GCGAAGTGCT
    GGCAGCTTGA AACGTCACAG CATTAAGAAG ATTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003642464.1
    CDS1..8439
    Translation

    Target ORF information:

    RefSeq Version XM_003642416.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofibromin 1 (NF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003642416.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGAGTCTGAC ATTTAAAGAG AAAATGACAA GCCTTAAATT TAAAGAAAAG
    CCTACTGACT TGGAAACCAA AAGCTACAAG TTCCTGCTGT TATCCTTGGT AAAACTGATC
    CATGCTGATC CAAAGCTTTT GCTGTGTAAT CCAAGGAAGC AAGGGCCTGA GACACAAGGC
    AGCACTGCTG AATTAATTAC AGGCCTTGTA CAACTCGTTC CACAATCCAG CATGCCAGAT
    ATAGCACAAG AAGCCATGGA GGCCTTGCTA GTTCTTCACC AGTTAGACAG CATTGACTTA
    TGGAATCCTG ATGCACCTAT AGAAACTTTC TGGGAAATTA GTTCACAAAT GCTTTTTTAT
    ATCTGCAAGA AATTAACCAG TCATCAGATG CTCAGCAGTA CAGAAATCCT CAAGTGGCTG
    CGAGAGATTC TCATCTGTAG GAATAAATTT CTTCTAAAAA ATAAACAATC AGACAGAACT
    TCCTGTCACT TTCTCTTCCT TTATGATGTC TCTGGAGGTG GAGCTAGTCA GATGTCCCTT
    GACCACGAAG AGATGATGCG CTGTGCTCCC GGAGCCACCC TCAGAAAAGG AAAAGGGAAT
    TCTTCCATGG AAAGTACAGC TGGATGCAGT GGAACACCCA TTTGTCGCCA GGCCCAAACA
    AAATTGGAAG TTGCCTTATA CATGTTTTTG TGGAGTCCGG ATATTGAGGC AGTCCTTGTT
    GCAATGTCCT GTTTCCGTCA CCTCTGTGAG GAAGCAGATA TCAGATGTGG AGTAGATGAA
    GTTTCAGTTC ATAATTTTTT GCCAAACTAC AACACTTTCA TGGAGTTTGC ATCTGTTAGC
    AATATGATGT CAACAGGGAG AGCAGCTCTT CAGAAAAGAG TGATGGCTTT ATTAAGACGT
    ATTGAACATC CAACTGCAGG GAACACAGAG GCTTGGGAGG ATACACATGC AAAATGGGAA
    CAAGCTACAA AACTAATCCT TAACTATCCA AAGACCAAAA TGGAAGACGG GCAGGTTACT
    GAAAGTCTTC ATAAGACAAT TGTTAAGAGA CGAATGTCAC ATGTTAGTGG GGGAGGCTCC
    ATAGACTTGT CTGACACAGA TTCTCTTCAG GAATGGATCA ACATGACGGG ATTCCTCTGT
    GCCCTCGGAG GAGTGTGCCT GCAGCATCGT AGCAATGCAG GATTAGCAAC CTACAGTCCA
    CCCATGGGCC CAGTCAATGA GCGTAAGGGC TCCATGATAT CTATGGTATC CACTGAGGGA
    AATGCAGAGA CTCCTGTTAG CAAATTCATA GATCGATTAC TGACTCTGAT GGTCTGTAAC
    CATGAGAAAG TGGGACTACA AATACGGACT AATATTAAAG ATCTGGTGGG TTTGGAGTTG
    AGTCCAGCAC TTTATCCAAT GCTATTTAAC AAATTGAAGA ATACCATCAG CAAGTTTTTT
    GATTCTCAAG GACAGGTTTT GTTAACTGAG ACCAACACAC AGTTTGTAGA ACAAACCATT
    GCTATAATGA AGAACTTGCT TGACAATCAT ACAGAAGGGA GCTCAGAACA TCTTGGACAA
    GCTAGTATTG AGACAATGAT GCTAAACCTG GTTAGGTATG TGCGTGTACT TGGAAATTTG
    GTACATGCAA TTCAAATAAA AACTAAACTC TGTCAGTTGG TGGAAGTAAT GATGGAGCGG
    AGAGATGACC TTTCATTTTG CCAAGAAATG AAATTTAGGA ACAAAATGGT GGAATATCTG
    ACAGACTGGG TTATGGGAAC ATCTAATCAA GCAACAGATG AAGATGTGAA GTGCTTGACA
    AGAGATTTGG ACCAAGCAAG TATGGAAGCA GTGGTCTCTC TTCTTGCTGG GCTTCCGTTA
    CAGCCTGAAG AAGGAGATGG TGTTGAGTTG ATGGAAGCCA AATCTCAGTT ATTTCTTAAA
    TATTTCACAC TGTTTATGAA CCTTCTAAAT GACTGTAGTG AAGTTGAAGA TGAAAGTGCA
    CAAACAGGTG GCAGGAAACG TGGGATGTCC CGAAGACTGG CTTCGCTGCG GCACTGCACT
    GTTCTTGCTA TGTCAAATTT ACTAAATGCA AATGTGGACA GTGGTCTTAT GCATTCAATA
    GGCTTGGGCT ATCATAAAGA CCTCCAGACA AGAGCTACAT TTATGGAAGT CCTCACCAAA
    ATTCTACAGC AGGGAACAGA ATTTGATACG TTAGCAGAAA CGGTGCTGGC AGATCGGTTT
    GAGAGGCTGG TGGAGCTAGT TACAATGATG GGTGATCAGG GGGAGCTTCC TATTGCTATG
    GCTTTAGCAA ATGTGGTGCC TTGTTCTCAG TGGGATGAGC TAGCTCGTGT TCTGGTCACA
    CTCTTTGATT CCCGGCACTT GCTCTACCAG CTACTTTGGA ATATGTTTTC AAAGGAAGTT
    GAACTGGCAG ATTCCATGCA GACACTCTTC CGAGGAAACA GCTTAGCTAG TAAAATAATG
    ACTTTCTGTT TTAAGGTATA TGGTGCTACT TATTTGCAGA AGCTTTTGGA ACCTTTATTA
    AGAACAGTGA TCACATCCTC TGAATGGCAG CATGTTAGCT TTGAGGTGGA TCCTACAAGA
    CTGGAGCCTA CAGAAAGCCT TGAAGAGAAC CAGCGGAGTC TCTTGCAAAT GACAGAAAAA
    TTTTTTCATG CAATCATTAA TTCTTCTTCG GAATTCCCAC CACAACTTCG CAGTGTGTGC
    CATTGTTTGT ACCAGGTTGT TAGTCAGCGA TTTCCTCAGA ACAGCATTGG AGCAGTTGGG
    AGTGCCATGT TCCTGCGGTT CATCAACCCT GCGATCGTCT CCCCGTACGA GGCAGGGATT
    TTAGATAAAA AGCCTCCGCC CAGAATTGAG AGGGGACTGA AACTGATGTC AAAGATACTT
    CAGAGTATTG CCAACCACGT CCTGTTTACA AAAGAAGAAC ATATGAGGCC TTTTAATGAC
    TTCGTAAAAA GCAATTTTGA TGCAGCTCGA AGGTTTTTCC TTGATATTGC ATCTGATTGC
    CCTGCGAGTG ACACTGTCAA TCACAGCCTG TCTTTCATCA GTGATGGCAA TGTTCTTGCT
    TTGCACCGGT TGTTGTGGAA CAATCAGGAG AAAATTGGCC AGTACCTTTC CAGTAATAGG
    GACCACAAAG CTGTTGGAAG GCGACCTTTT GACAAAATGG CTACTCTTCT TGCCTATCTG
    GGTCCACCAG AGCACAAACC TGTGGCAGAT ACACATTGGT CCAGTTTAAA CCTCACCAGT
    TCGAAATTTG AAGAATTTAT GACAAGGCAT CAGGTACATG AAAAAGAAGA GTTCAAAGCA
    TTGAAAACGT TAAATATTTT CTACCAGGCT GGGACATCCA AAGCTGGCAA TCCTGTTTTC
    TACTACATTG CTCGGCGTTT CAAGACTGGT CAGATCAATG GAGATTTATT GATATACCAC
    GTCCTGCTAA CTCTGAAGCC ATACTACGCA AAGCCATATG AAATTGTAGT GGACCTCACA
    CATGCTGGCC CCAGTAATCG CTTTAAAACA GACTTTCTTT CTAAATGGTT TGTAGTTTTT
    CCAGGCTTTG CTTATGAAAA TGTCTCAGCA GTTTTTATTT ATAACTGTAA TTCTTGGGTC
    AGAGAATACA CCAAATACCA CGAAAGACTC TTGACAGGTT TGAAAGGAAG CAAAAGGCTT
    ATTTTCATAG ACTCTCCAGG AAAACTGGCG GAGCACATTG AACATGACCA GCAGAAACTT
    CCAGCAGCTA CCTTAGCTTT GGAAGAGGAC TTGAAAGTAT TTCACAATGC CCTCAAATTG
    GCTCACAAAG ACACCAAAGT GTCTATTAAA GTTGGATCAA CAGCTGTGCA GGTGACATCA
    GCAGAACGAA CAAGAGTCCT GGGACAGACA GTGTTTTTAA ATGATATATA CTACGCCTCT
    GAAATTGAGG AAATATGTCT TGTTGATGAG AATCAGTTTA CATTGACGAT TGCCAACCAG
    GGAACGCCAC TCACCTTCAT GCATCAGGAG TGTGAAGCCA TTGTGAGGTC CATTATTCAT
    ATACGGACAC GGTGGGAGCT GTCGCAACCA GACTCCATCC CACAGCACAC TAAAATTCGC
    CCTAAGGATG TTCCCGGAAC ACTACTGAAC ATAGCATTGC TCAACCTGGG AAGCTCAGAC
    CCGAGTTTGC GGTCTGCTGC CTATAATCTT CTGTGTGCCT TAACCTGTAC CTTTAATTTA
    AAGATTGAGG GCCAGTTACT GGAAACTTCA GGTCTGTGTA TTCCTGCCAA CAACACGCTC
    TTTATTGTGT CTATTAGTAA GACTCTTGCA GCTAATGAGC CACACCTTAC CCTAGAGTTC
    TTGGAAGAGT GTATTTCAGG ATTTAGCAAA TCTAGTATTG AACTGAAACA TCTTTGTCTG
    GAATATATGA CTCCCTGGCT GTCCAATCTG GTCCGATTCT GCAAACACAA CGACGATGCG
    AAGCGCCAGC GAGTCACTGC CATTCTTGAT AAGCTTATAA CAATGACAAT AAATGAAAAA
    CAGATGTACC CTTCTATTCA GGCTAAAATA TGGGGAAGTC TTGGACAGAT TACAGATCTC
    CTTGACGTAG TGCTGGACAG TTTCATCAAA ACTAGTGCCA CGGGGGGCTT AGGATCCATT
    AAAGCTGAGG TTATGGCAGA CACAGCAGTA GCGCTTGCTT CTGGAAATGT AAAATTGGTT
    TCAAGCAAAG TGATTGGAAG GATGTGCAAA ATAATTGACA AGACTTGTCT GTCTCCAACT
    CCTACACTGG AACAGCACCT GATGTGGGAT GATATTGCCA TTCTAGCACG TTACATGCTG
    ATGCTCTCCT TCAACAATTC TCTGGATGTG GCTGCACATC TCCCCTACCT CTTTCATGTG
    GTTACTTTGT TGGTGGCCAC TGGACCCCTT TCTCTCAGAG CTTCCACTCA TGGTCTTGTC
    ATCAACATCA TTCATTCTCT GTGCACTTGC TCACAGCTTC ATTTTAGTGA GGAGACCAAG
    CAAGTTTTAA GACTGAGCTT GACTGAATTT TCTCTGCCTA AATTTTATTT GCTGTTTGGA
    ATCAGCAAAG TGAAATCAGC TGCAGTCATA GCATTCCGCT CCAGCTATCG AGACAGGTCA
    TTTTCACCTG GCTCCTACGA GAGGGAGACT TTTGCACTGA CCTCCTTGGA AACTGTTACT
    GAAGCTTTGC TGGAGATCAT GGAGGCTTGT ATGAGAGATA TTCCAACATG CAAGTGGCTG
    GACCAGTGGA CCGAACTAGC ACAAAAGTTT GCATTCCAGT ACAATCCATC CCTGCAGCCA
    AGAGCACTCG TTGTCTTTGG CTGTATAAGT AAGCGTGTCT CTCATGGACA AATAAAACAA
    ATAATCCGAA TTCTTAGCAA GGGGCTTGAG AGCTGTTTAA AAGGCCCTGA TAATTACAAT
    AGCCAAGTTC TAATAGAAGC TACGGTTATA GCACTCACCA AACTGCAGCC TCTTCTTAAC
    AAGGACTCCC CCATGCACAA GGCCCTCTTC TGGGTAGCCA TGGCAGTACT GCAGTTGGAT
    GAAGTCAACC TATACTCAGC AGGTACAGCA CTGCTTGAAC AGAATCTGCA TACCTTGGAT
    AGCCTTCGAG TGTTCAATGA TAAGAGCCCA GAAGAGGTGT TCATGGAGAT CAGGAGACCA
    CTTGAATGGC ACTGCAAACA GATGGATCAT TTTGTTGGGC TAAATTTCAA CTCCAACTTC
    AACTTTGCAC TAGTGGGGCA CCTCCTGAAA GGGTACAGGC ATCCTTCACC TACCACTGTA
    GCAAGAACAG TAAGGATTCT ACATACACTA CTAGCTCTGG TTAACAAACA CAGGAACTGT
    GACAAGTTTG AGGTGAACAC CCAGAGCGTG GCTTACCTAG CAGCTTTGCT GACAGTGTCT
    GAGGAAGTTC GAAGTCGCTG CAGCCTAAAA CATAGGAAGT CTCTCTTGCT GACAGATGTT
    GCAATGGAAA ATGTTCCTAT GGATACATAT CCCATACATC ACAGTGACAC TACCTATAGG
    ACACTAAAGG AAAATCAACC ATGGTCATCA CCAAAGGGAT CAGACAGACA CCTGGCTGCC
    AGTTACCCGA CAGTGGGACA GATAAGCCCT CGAACACGAA AGTCCATGAG TTTGGATATG
    GGGCAGCCTT CACAAGCGAA CACTAAAAAG CTTCTAGGTA CAAGGAAAAG TTTTGACCAT
    TTGATATCGG ACACAAAGGC TCCTAAAAGG CAAGACATGG AATCTGGAAT CACAACGCCT
    CCCAAAATGA GGAGAGTAGC CGAAAATGAT TATGAAATGG AAACCCAGAG AATATCACCG
    CAGCAGCACC CCCATCTCCG GAAAGTTTCT GTTTCTGAGT CAAATGTCCT CCTGGATGAA
    GAAGTTCTGA CTGATCCAAA AATTCAAGCA CTGCTGCTTA CAGTTCTTGC CACGTTAGTG
    AAGTACACTA CTGATGAATT TGACCAGCGG ATTCTGTATG AGTATTTAGC AGAAGCCAGT
    GTTGTCTTCC CAAAGGTCTT TCCTGTTGTG CATAATTTAT TGGATTCCAA GATCAATACC
    CTCTTGTCGC TGTGTCAAGA TCCAAATTTG TTGAATCCAA TTCATGGGAT TGTTCAGAGT
    GTTGTCTACC ATGAGGAGTC TCCACCCCAA TACCAAACTT CTTATCTACA AAGTTTTGGA
    TTTAATGGGT TGTGGAGGTT TGCTGGCCCA TTTTCCAAGC AAACACAAAT CCCAGACTAT
    GCTGAGCTCA TAGTGAAGTT TCTTGATGCC TTGATTGACA CGTATTTGCC TGGAATTGAT
    GAGGAAACCA GTGAAGAATC TCTCCTGACA CCCACGTCTC CATATCCTCC CGCAGTGCAG
    AGCCAGCTTA GCATTACAGC TAACCTTAAC CTTTCCAATT CCATGACCTC GCTCGCAACT
    TCCCAGCACT CCCCAGGGAT CGACAAGGAG AACGTGGAGC TCTCGCCCAC CACTGGCCAC
    ACCAACAGCG GGAGGATGCG CCACGGCTCC GCCAGCCAGG TGCAAAAGCA GCGAAGTGCT
    GGCAGCTTGA AACGTCACAG CATTAAGAAG ATTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa44369
    Clone ID Related Accession (Same CDS sequence) XM_015295852.1 , XM_015295853.1
    Accession Version XM_015295852.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8598bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofibromin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455988.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGATGCCAAC CAGTCACTGT TTTGCATGGC AGTGGGCCTC TGTTGCTAGT
    CTGACATTTA AAGAGAAAAT GACAAGCCTT AAATTTAAAG AAAAGCCTAC TGACTTGGAA
    ACCAAAAGCT ACAAGTTCCT GCTGTTATCC TTGGTAAAAC TGATCCATGC TGATCCAAAG
    CTTTTGCTGT GTAATCCAAG GAAGCAAGGG CCTGAGACAC AAGGCAGCAC TGCTGAATTA
    ATTACAGGCC TTGTACAACT CGTTCCACAA TCCAGCATGC CAGATATAGC ACAAGAAGCC
    ATGGAGGCCT TGCTAGTTCT TCACCAGTTA GACAGCATTG ACTTATGGAA TCCTGATGCA
    CCTATAGAAA CTTTCTGGGA AATTAGTTCA CAAATGCTTT TTTATATCTG CAAGAAATTA
    ACCAGTCATC AGATGCTCAG CAGTACAGAA ATCCTCAAGT GGCTGCGAGA GATTCTCATC
    TGTAGGAATA AATTTCTTCT AAAAAATAAA CAATCAGACA GAACTTCCTG TCACTTTCTC
    TTCCTTTATG ATGTCTCTGG AGGTGGAGCT AGTCAGATGT CCCTTGACCA CGAAGAGATG
    ATGCGCTGTG CTCCCGGAGC CACCCTCAGA AAAGGAAAAG GGAATTCTTC CATGGAAAGT
    ACAGCTGGAT GCAGTGGAAC ACCCATTTGT CGCCAGGCCC AAACAAAATT GGAAGTTGCC
    TTATACATGT TTTTGTGGAG TCCGGATATT GAGGCAGTCC TTGTTGCAAT GTCCTGTTTC
    CGTCACCTCT GTGAGGAAGC AGATATCAGA TGTGGAGTAG ATGAAGTTTC AGTTCATAAT
    TTTTTGCCAA ACTACAACAC TTTCATGGAG TTTGCATCTG TTAGCAATAT GATGTCAACA
    GGGAGAGCAG CTCTTCAGAA AAGAGTGATG GCTTTATTAA GACGTATTGA ACATCCAACT
    GCAGGGAACA CAGAGGCTTG GGAGGATACA CATGCAAAAT GGGAACAAGC TACAAAACTA
    ATCCTTAACT ATCCAAAGAC CAAAATGGAA GACGGGCAGG TTACTGAAAG TCTTCATAAG
    ACAATTGTTA AGAGACGAAT GTCACATGTT AGTGGGGGAG GCTCCATAGA CTTGTCTGAC
    ACAGATTCTC TTCAGGAATG GATCAACATG ACGGGATTCC TCTGTGCCCT CGGAGGAGTG
    TGCCTGCAGC ATCGTAGCAA TGCAGGATTA GCAACCTACA GTCCACCCAT GGGCCCAGTC
    AATGAGCGTA AGGGCTCCAT GATATCTATG GTATCCACTG AGGGAAATGC AGAGACTCCT
    GTTAGCAAAT TCATAGATCG ATTACTGACT CTGATGGTCT GTAACCATGA GAAAGTGGGA
    CTACAAATAC GGACTAATAT TAAAGATCTG GTGGGTTTGG AGTTGAGTCC AGCACTTTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGATTC TCAAGGACAG
    GTTTTGTTAA CTGAGACCAA CACACAGTTT GTAGAACAAA CCATTGCTAT AATGAAGAAC
    TTGCTTGACA ATCATACAGA AGGGAGCTCA GAACATCTTG GACAAGCTAG TATTGAGACA
    ATGATGCTAA ACCTGGTTAG GTATGTGCGT GTACTTGGAA ATTTGGTACA TGCAATTCAA
    ATAAAAACTA AACTCTGTCA GTTGGTGGAA GTAATGATGG AGCGGAGAGA TGACCTTTCA
    TTTTGCCAAG AAATGAAATT TAGGAACAAA ATGGTGGAAT ATCTGACAGA CTGGGTTATG
    GGAACATCTA ATCAAGCAAC AGATGAAGAT GTGAAGTGCT TGACAAGAGA TTTGGACCAA
    GCAAGTATGG AAGCAGTGGT CTCTCTTCTT GCTGGGCTTC CGTTACAGCC TGAAGAAGGA
    GATGGTGTTG AGTTGATGGA AGCCAAATCT CAGTTATTTC TTAAATATTT CACACTGTTT
    ATGAACCTTC TAAATGACTG TAGTGAAGTT GAAGATGAAA GTGCACAAAC AGGTGGCAGG
    AAACGTGGGA TGTCCCGAAG ACTGGCTTCG CTGCGGCACT GCACTGTTCT TGCTATGTCA
    AATTTACTAA ATGCAAATGT GGACAGTGGT CTTATGCATT CAATAGGCTT GGGCTATCAT
    AAAGACCTCC AGACAAGAGC TACATTTATG GAAGTCCTCA CCAAAATTCT ACAGCAGGGA
    ACAGAATTTG ATACGTTAGC AGAAACGGTG CTGGCAGATC GGTTTGAGAG GCTGGTGGAG
    CTAGTTACAA TGATGGGTGA TCAGGGGGAG CTTCCTATTG CTATGGCTTT AGCAAATGTG
    GTGCCTTGTT CTCAGTGGGA TGAGCTAGCT CGTGTTCTGG TCACACTCTT TGATTCCCGG
    CACTTGCTCT ACCAGCTACT TTGGAATATG TTTTCAAAGG AAGTTGAACT GGCAGATTCC
    ATGCAGACAC TCTTCCGAGG AAACAGCTTA GCTAGTAAAA TAATGACTTT CTGTTTTAAG
    GTATATGGTG CTACTTATTT GCAGAAGCTT TTGGAACCTT TATTAAGAAC AGTGATCACA
    TCCTCTGAAT GGCAGCATGT TAGCTTTGAG GTGGATCCTA CAAGACTGGA GCCTACAGAA
    AGCCTTGAAG AGAACCAGCG GAGTCTCTTG CAAATGACAG AAAAATTTTT TCATGCAATC
    ATTAATTCTT CTTCGGAATT CCCACCACAA CTTCGCAGTG TGTGCCATTG TTTGTACCAG
    GCAACTTGCC ACTCTCTACT GAATAAAGCT ACCGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTTGTTA GTCAGCGATT TCCTCAGAAC AGCATTGGAG CAGTTGGGAG TGCCATGTTC
    CTGCGGTTCA TCAACCCTGC GATCGTCTCC CCGTACGAGG CAGGGATTTT AGATAAAAAG
    CCTCCGCCCA GAATTGAGAG GGGACTGAAA CTGATGTCAA AGATACTTCA GAGTATTGCC
    AACCACGTCC TGTTTACAAA AGAAGAACAT ATGAGGCCTT TTAATGACTT CGTAAAAAGC
    AATTTTGATG CAGCTCGAAG GTTTTTCCTT GATATTGCAT CTGATTGCCC TGCGAGTGAC
    ACTGTCAATC ACAGCCTGTC TTTCATCAGT GATGGCAATG TTCTTGCTTT GCACCGGTTG
    TTGTGGAACA ATCAGGAGAA AATTGGCCAG TACCTTTCCA GTAATAGGGA CCACAAAGCT
    GTTGGAAGGC GACCTTTTGA CAAAATGGCT ACTCTTCTTG CCTATCTGGG TCCACCAGAG
    CACAAACCTG TGGCAGATAC ACATTGGTCC AGTTTAAACC TCACCAGTTC GAAATTTGAA
    GAATTTATGA CAAGGCATCA GGTACATGAA AAAGAAGAGT TCAAAGCATT GAAAACGTTA
    AATATTTTCT ACCAGGCTGG GACATCCAAA GCTGGCAATC CTGTTTTCTA CTACATTGCT
    CGGCGTTTCA AGACTGGTCA GATCAATGGA GATTTATTGA TATACCACGT CCTGCTAACT
    CTGAAGCCAT ACTACGCAAA GCCATATGAA ATTGTAGTGG ACCTCACACA TGCTGGCCCC
    AGTAATCGCT TTAAAACAGA CTTTCTTTCT AAATGGTTTG TAGTTTTTCC AGGCTTTGCT
    TATGAAAATG TCTCAGCAGT TTTTATTTAT AACTGTAATT CTTGGGTCAG AGAATACACC
    AAATACCACG AAAGACTCTT GACAGGTTTG AAAGGAAGCA AAAGGCTTAT TTTCATAGAC
    TCTCCAGGAA AACTGGCGGA GCACATTGAA CATGACCAGC AGAAACTTCC AGCAGCTACC
    TTAGCTTTGG AAGAGGACTT GAAAGTATTT CACAATGCCC TCAAATTGGC TCACAAAGAC
    ACCAAAGTGT CTATTAAAGT TGGATCAACA GCTGTGCAGG TGACATCAGC AGAACGAACA
    AGAGTCCTGG GACAGACAGT GTTTTTAAAT GATATATACT ACGCCTCTGA AATTGAGGAA
    ATATGTCTTG TTGATGAGAA TCAGTTTACA TTGACGATTG CCAACCAGGG AACGCCACTC
    ACCTTCATGC ATCAGGAGTG TGAAGCCATT GTGAGGTCCA TTATTCATAT ACGGACACGG
    TGGGAGCTGT CGCAACCAGA CTCCATCCCA CAGCACACTA AAATTCGCCC TAAGGATGTT
    CCCGGAACAC TACTGAACAT AGCATTGCTC AACCTGGGAA GCTCAGACCC GAGTTTGCGG
    TCTGCTGCCT ATAATCTTCT GTGTGCCTTA ACCTGTACCT TTAATTTAAA GATTGAGGGC
    CAGTTACTGG AAACTTCAGG TCTGTGTATT CCTGCCAACA ACACGCTCTT TATTGTGTCT
    ATTAGTAAGA CTCTTGCAGC TAATGAGCCA CACCTTACCC TAGAGTTCTT GGAAGAGTGT
    ATTTCAGGAT TTAGCAAATC TAGTATTGAA CTGAAACATC TTTGTCTGGA ATATATGACT
    CCCTGGCTGT CCAATCTGGT CCGATTCTGC AAACACAACG ACGATGCGAA GCGCCAGCGA
    GTCACTGCCA TTCTTGATAA GCTTATAACA ATGACAATAA ATGAAAAACA GATGTACCCT
    TCTATTCAGG CTAAAATATG GGGAAGTCTT GGACAGATTA CAGATCTCCT TGACGTAGTG
    CTGGACAGTT TCATCAAAAC TAGTGCCACG GGGGGCTTAG GATCCATTAA AGCTGAGGTT
    ATGGCAGACA CAGCAGTAGC GCTTGCTTCT GGAAATGTAA AATTGGTTTC AAGCAAAGTG
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACTTGTCTGT CTCCAACTCC TACACTGGAA
    CAGCACCTGA TGTGGGATGA TATTGCCATT CTAGCACGTT ACATGCTGAT GCTCTCCTTC
    AACAATTCTC TGGATGTGGC TGCACATCTC CCCTACCTCT TTCATGTGGT TACTTTGTTG
    GTGGCCACTG GACCCCTTTC TCTCAGAGCT TCCACTCATG GTCTTGTCAT CAACATCATT
    CATTCTCTGT GCACTTGCTC ACAGCTTCAT TTTAGTGAGG AGACCAAGCA AGTTTTAAGA
    CTGAGCTTGA CTGAATTTTC TCTGCCTAAA TTTTATTTGC TGTTTGGAAT CAGCAAAGTG
    AAATCAGCTG CAGTCATAGC ATTCCGCTCC AGCTATCGAG ACAGGTCATT TTCACCTGGC
    TCCTACGAGA GGGAGACTTT TGCACTGACC TCCTTGGAAA CTGTTACTGA AGCTTTGCTG
    GAGATCATGG AGGCTTGTAT GAGAGATATT CCAACATGCA AGTGGCTGGA CCAGTGGACC
    GAACTAGCAC AAAAGTTTGC ATTCCAGTAC AATCCATCCC TGCAGCCAAG AGCACTCGTT
    GTCTTTGGCT GTATAAGTAA GCGTGTCTCT CATGGACAAA TAAAACAAAT AATCCGAATT
    CTTAGCAAGG GGCTTGAGAG CTGTTTAAAA GGCCCTGATA ATTACAATAG CCAAGTTCTA
    ATAGAAGCTA CGGTTATAGC ACTCACCAAA CTGCAGCCTC TTCTTAACAA GGACTCCCCC
    ATGCACAAGG CCCTCTTCTG GGTAGCCATG GCAGTACTGC AGTTGGATGA AGTCAACCTA
    TACTCAGCAG GTACAGCACT GCTTGAACAG AATCTGCATA CCTTGGATAG CCTTCGAGTG
    TTCAATGATA AGAGCCCAGA AGAGGTGTTC ATGGAGATCA GGAGACCACT TGAATGGCAC
    TGCAAACAGA TGGATCATTT TGTTGGGCTA AATTTCAACT CCAACTTCAA CTTTGCACTA
    GTGGGGCACC TCCTGAAAGG GTACAGGCAT CCTTCACCTA CCACTGTAGC AAGAACAGTA
    AGGATTCTAC ATACACTACT AGCTCTGGTT AACAAACACA GGAACTGTGA CAAGTTTGAG
    GTGAACACCC AGAGCGTGGC TTACCTAGCA GCTTTGCTGA CAGTGTCTGA GGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGGAAGTCT CTCTTGCTGA CAGATGTTGC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATACATCAC AGTGACACTA CCTATAGGAC ACTAAAGGAA
    AATCAACCAT GGTCATCACC AAAGGGATCA GACAGACACC TGGCTGCCAG TTACCCGACA
    GTGGGACAGA TAAGCCCTCG AACACGAAAG TCCATGAGTT TGGATATGGG GCAGCCTTCA
    CAAGCGAACA CTAAAAAGCT TCTAGGTACA AGGAAAAGTT TTGACCATTT GATATCGGAC
    ACAAAGGCTC CTAAAAGGCA AGACATGGAA TCTGGAATCA CAACGCCTCC CAAAATGAGG
    AGAGTAGCCG AAAATGATTA TGAAATGGAA ACCCAGAGAA TATCACCGCA GCAGCACCCC
    CATCTCCGGA AAGTTTCTGT TTCTGAGTCA AATGTCCTCC TGGATGAAGA AGTTCTGACT
    GATCCAAAAA TTCAAGCACT GCTGCTTACA GTTCTTGCCA CGTTAGTGAA GTACACTACT
    GATGAATTTG ACCAGCGGAT TCTGTATGAG TATTTAGCAG AAGCCAGTGT TGTCTTCCCA
    AAGGTCTTTC CTGTTGTGCA TAATTTATTG GATTCCAAGA TCAATACCCT CTTGTCGCTG
    TGTCAAGATC CAAATTTGTT GAATCCAATT CATGGGATTG TTCAGAGTGT TGTCTACCAT
    GAGGAGTCTC CACCCCAATA CCAAACTTCT TATCTACAAA GTTTTGGATT TAATGGGTTG
    TGGAGGTTTG CTGGCCCATT TTCCAAGCAA ACACAAATCC CAGACTATGC TGAGCTCATA
    GTGAAGTTTC TTGATGCCTT GATTGACACG TATTTGCCTG GAATTGATGA GGAAACCAGT
    GAAGAATCTC TCCTGACACC CACGTCTCCA TATCCTCCCG CAGTGCAGAG CCAGCTTAGC
    ATTACAGCTA ACCTTAACCT TTCCAATTCC ATGACCTCGC TCGCAACTTC CCAGCACTCC
    CCAGCTTCTC TGCCTTGCTC TAAATCTGCA GTTTTCATGC AGCTTCCTCA TCAAGGGATC
    GACAAGGAGA ACGTGGAGCT CTCGCCCACC ACTGGCCACA CCAACAGCGG GAGGATGCGC
    CACGGCTCCG CCAGCCAGGT GCAAAAGCAG CGAAGTGCTG GCAGCTTGAA ACGTCACAGC
    ATTAAGAAGA TTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015151338.1
    CDS217..8814
    Translation

    Target ORF information:

    RefSeq Version XM_015295852.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofibromin 1 (NF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015295852.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGATGCCAAC CAGTCACTGT TTTGCATGGC AGTGGGCCTC TGTTGCTAGT
    CTGACATTTA AAGAGAAAAT GACAAGCCTT AAATTTAAAG AAAAGCCTAC TGACTTGGAA
    ACCAAAAGCT ACAAGTTCCT GCTGTTATCC TTGGTAAAAC TGATCCATGC TGATCCAAAG
    CTTTTGCTGT GTAATCCAAG GAAGCAAGGG CCTGAGACAC AAGGCAGCAC TGCTGAATTA
    ATTACAGGCC TTGTACAACT CGTTCCACAA TCCAGCATGC CAGATATAGC ACAAGAAGCC
    ATGGAGGCCT TGCTAGTTCT TCACCAGTTA GACAGCATTG ACTTATGGAA TCCTGATGCA
    CCTATAGAAA CTTTCTGGGA AATTAGTTCA CAAATGCTTT TTTATATCTG CAAGAAATTA
    ACCAGTCATC AGATGCTCAG CAGTACAGAA ATCCTCAAGT GGCTGCGAGA GATTCTCATC
    TGTAGGAATA AATTTCTTCT AAAAAATAAA CAATCAGACA GAACTTCCTG TCACTTTCTC
    TTCCTTTATG ATGTCTCTGG AGGTGGAGCT AGTCAGATGT CCCTTGACCA CGAAGAGATG
    ATGCGCTGTG CTCCCGGAGC CACCCTCAGA AAAGGAAAAG GGAATTCTTC CATGGAAAGT
    ACAGCTGGAT GCAGTGGAAC ACCCATTTGT CGCCAGGCCC AAACAAAATT GGAAGTTGCC
    TTATACATGT TTTTGTGGAG TCCGGATATT GAGGCAGTCC TTGTTGCAAT GTCCTGTTTC
    CGTCACCTCT GTGAGGAAGC AGATATCAGA TGTGGAGTAG ATGAAGTTTC AGTTCATAAT
    TTTTTGCCAA ACTACAACAC TTTCATGGAG TTTGCATCTG TTAGCAATAT GATGTCAACA
    GGGAGAGCAG CTCTTCAGAA AAGAGTGATG GCTTTATTAA GACGTATTGA ACATCCAACT
    GCAGGGAACA CAGAGGCTTG GGAGGATACA CATGCAAAAT GGGAACAAGC TACAAAACTA
    ATCCTTAACT ATCCAAAGAC CAAAATGGAA GACGGGCAGG TTACTGAAAG TCTTCATAAG
    ACAATTGTTA AGAGACGAAT GTCACATGTT AGTGGGGGAG GCTCCATAGA CTTGTCTGAC
    ACAGATTCTC TTCAGGAATG GATCAACATG ACGGGATTCC TCTGTGCCCT CGGAGGAGTG
    TGCCTGCAGC ATCGTAGCAA TGCAGGATTA GCAACCTACA GTCCACCCAT GGGCCCAGTC
    AATGAGCGTA AGGGCTCCAT GATATCTATG GTATCCACTG AGGGAAATGC AGAGACTCCT
    GTTAGCAAAT TCATAGATCG ATTACTGACT CTGATGGTCT GTAACCATGA GAAAGTGGGA
    CTACAAATAC GGACTAATAT TAAAGATCTG GTGGGTTTGG AGTTGAGTCC AGCACTTTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGATTC TCAAGGACAG
    GTTTTGTTAA CTGAGACCAA CACACAGTTT GTAGAACAAA CCATTGCTAT AATGAAGAAC
    TTGCTTGACA ATCATACAGA AGGGAGCTCA GAACATCTTG GACAAGCTAG TATTGAGACA
    ATGATGCTAA ACCTGGTTAG GTATGTGCGT GTACTTGGAA ATTTGGTACA TGCAATTCAA
    ATAAAAACTA AACTCTGTCA GTTGGTGGAA GTAATGATGG AGCGGAGAGA TGACCTTTCA
    TTTTGCCAAG AAATGAAATT TAGGAACAAA ATGGTGGAAT ATCTGACAGA CTGGGTTATG
    GGAACATCTA ATCAAGCAAC AGATGAAGAT GTGAAGTGCT TGACAAGAGA TTTGGACCAA
    GCAAGTATGG AAGCAGTGGT CTCTCTTCTT GCTGGGCTTC CGTTACAGCC TGAAGAAGGA
    GATGGTGTTG AGTTGATGGA AGCCAAATCT CAGTTATTTC TTAAATATTT CACACTGTTT
    ATGAACCTTC TAAATGACTG TAGTGAAGTT GAAGATGAAA GTGCACAAAC AGGTGGCAGG
    AAACGTGGGA TGTCCCGAAG ACTGGCTTCG CTGCGGCACT GCACTGTTCT TGCTATGTCA
    AATTTACTAA ATGCAAATGT GGACAGTGGT CTTATGCATT CAATAGGCTT GGGCTATCAT
    AAAGACCTCC AGACAAGAGC TACATTTATG GAAGTCCTCA CCAAAATTCT ACAGCAGGGA
    ACAGAATTTG ATACGTTAGC AGAAACGGTG CTGGCAGATC GGTTTGAGAG GCTGGTGGAG
    CTAGTTACAA TGATGGGTGA TCAGGGGGAG CTTCCTATTG CTATGGCTTT AGCAAATGTG
    GTGCCTTGTT CTCAGTGGGA TGAGCTAGCT CGTGTTCTGG TCACACTCTT TGATTCCCGG
    CACTTGCTCT ACCAGCTACT TTGGAATATG TTTTCAAAGG AAGTTGAACT GGCAGATTCC
    ATGCAGACAC TCTTCCGAGG AAACAGCTTA GCTAGTAAAA TAATGACTTT CTGTTTTAAG
    GTATATGGTG CTACTTATTT GCAGAAGCTT TTGGAACCTT TATTAAGAAC AGTGATCACA
    TCCTCTGAAT GGCAGCATGT TAGCTTTGAG GTGGATCCTA CAAGACTGGA GCCTACAGAA
    AGCCTTGAAG AGAACCAGCG GAGTCTCTTG CAAATGACAG AAAAATTTTT TCATGCAATC
    ATTAATTCTT CTTCGGAATT CCCACCACAA CTTCGCAGTG TGTGCCATTG TTTGTACCAG
    GCAACTTGCC ACTCTCTACT GAATAAAGCT ACCGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTTGTTA GTCAGCGATT TCCTCAGAAC AGCATTGGAG CAGTTGGGAG TGCCATGTTC
    CTGCGGTTCA TCAACCCTGC GATCGTCTCC CCGTACGAGG CAGGGATTTT AGATAAAAAG
    CCTCCGCCCA GAATTGAGAG GGGACTGAAA CTGATGTCAA AGATACTTCA GAGTATTGCC
    AACCACGTCC TGTTTACAAA AGAAGAACAT ATGAGGCCTT TTAATGACTT CGTAAAAAGC
    AATTTTGATG CAGCTCGAAG GTTTTTCCTT GATATTGCAT CTGATTGCCC TGCGAGTGAC
    ACTGTCAATC ACAGCCTGTC TTTCATCAGT GATGGCAATG TTCTTGCTTT GCACCGGTTG
    TTGTGGAACA ATCAGGAGAA AATTGGCCAG TACCTTTCCA GTAATAGGGA CCACAAAGCT
    GTTGGAAGGC GACCTTTTGA CAAAATGGCT ACTCTTCTTG CCTATCTGGG TCCACCAGAG
    CACAAACCTG TGGCAGATAC ACATTGGTCC AGTTTAAACC TCACCAGTTC GAAATTTGAA
    GAATTTATGA CAAGGCATCA GGTACATGAA AAAGAAGAGT TCAAAGCATT GAAAACGTTA
    AATATTTTCT ACCAGGCTGG GACATCCAAA GCTGGCAATC CTGTTTTCTA CTACATTGCT
    CGGCGTTTCA AGACTGGTCA GATCAATGGA GATTTATTGA TATACCACGT CCTGCTAACT
    CTGAAGCCAT ACTACGCAAA GCCATATGAA ATTGTAGTGG ACCTCACACA TGCTGGCCCC
    AGTAATCGCT TTAAAACAGA CTTTCTTTCT AAATGGTTTG TAGTTTTTCC AGGCTTTGCT
    TATGAAAATG TCTCAGCAGT TTTTATTTAT AACTGTAATT CTTGGGTCAG AGAATACACC
    AAATACCACG AAAGACTCTT GACAGGTTTG AAAGGAAGCA AAAGGCTTAT TTTCATAGAC
    TCTCCAGGAA AACTGGCGGA GCACATTGAA CATGACCAGC AGAAACTTCC AGCAGCTACC
    TTAGCTTTGG AAGAGGACTT GAAAGTATTT CACAATGCCC TCAAATTGGC TCACAAAGAC
    ACCAAAGTGT CTATTAAAGT TGGATCAACA GCTGTGCAGG TGACATCAGC AGAACGAACA
    AGAGTCCTGG GACAGACAGT GTTTTTAAAT GATATATACT ACGCCTCTGA AATTGAGGAA
    ATATGTCTTG TTGATGAGAA TCAGTTTACA TTGACGATTG CCAACCAGGG AACGCCACTC
    ACCTTCATGC ATCAGGAGTG TGAAGCCATT GTGAGGTCCA TTATTCATAT ACGGACACGG
    TGGGAGCTGT CGCAACCAGA CTCCATCCCA CAGCACACTA AAATTCGCCC TAAGGATGTT
    CCCGGAACAC TACTGAACAT AGCATTGCTC AACCTGGGAA GCTCAGACCC GAGTTTGCGG
    TCTGCTGCCT ATAATCTTCT GTGTGCCTTA ACCTGTACCT TTAATTTAAA GATTGAGGGC
    CAGTTACTGG AAACTTCAGG TCTGTGTATT CCTGCCAACA ACACGCTCTT TATTGTGTCT
    ATTAGTAAGA CTCTTGCAGC TAATGAGCCA CACCTTACCC TAGAGTTCTT GGAAGAGTGT
    ATTTCAGGAT TTAGCAAATC TAGTATTGAA CTGAAACATC TTTGTCTGGA ATATATGACT
    CCCTGGCTGT CCAATCTGGT CCGATTCTGC AAACACAACG ACGATGCGAA GCGCCAGCGA
    GTCACTGCCA TTCTTGATAA GCTTATAACA ATGACAATAA ATGAAAAACA GATGTACCCT
    TCTATTCAGG CTAAAATATG GGGAAGTCTT GGACAGATTA CAGATCTCCT TGACGTAGTG
    CTGGACAGTT TCATCAAAAC TAGTGCCACG GGGGGCTTAG GATCCATTAA AGCTGAGGTT
    ATGGCAGACA CAGCAGTAGC GCTTGCTTCT GGAAATGTAA AATTGGTTTC AAGCAAAGTG
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACTTGTCTGT CTCCAACTCC TACACTGGAA
    CAGCACCTGA TGTGGGATGA TATTGCCATT CTAGCACGTT ACATGCTGAT GCTCTCCTTC
    AACAATTCTC TGGATGTGGC TGCACATCTC CCCTACCTCT TTCATGTGGT TACTTTGTTG
    GTGGCCACTG GACCCCTTTC TCTCAGAGCT TCCACTCATG GTCTTGTCAT CAACATCATT
    CATTCTCTGT GCACTTGCTC ACAGCTTCAT TTTAGTGAGG AGACCAAGCA AGTTTTAAGA
    CTGAGCTTGA CTGAATTTTC TCTGCCTAAA TTTTATTTGC TGTTTGGAAT CAGCAAAGTG
    AAATCAGCTG CAGTCATAGC ATTCCGCTCC AGCTATCGAG ACAGGTCATT TTCACCTGGC
    TCCTACGAGA GGGAGACTTT TGCACTGACC TCCTTGGAAA CTGTTACTGA AGCTTTGCTG
    GAGATCATGG AGGCTTGTAT GAGAGATATT CCAACATGCA AGTGGCTGGA CCAGTGGACC
    GAACTAGCAC AAAAGTTTGC ATTCCAGTAC AATCCATCCC TGCAGCCAAG AGCACTCGTT
    GTCTTTGGCT GTATAAGTAA GCGTGTCTCT CATGGACAAA TAAAACAAAT AATCCGAATT
    CTTAGCAAGG GGCTTGAGAG CTGTTTAAAA GGCCCTGATA ATTACAATAG CCAAGTTCTA
    ATAGAAGCTA CGGTTATAGC ACTCACCAAA CTGCAGCCTC TTCTTAACAA GGACTCCCCC
    ATGCACAAGG CCCTCTTCTG GGTAGCCATG GCAGTACTGC AGTTGGATGA AGTCAACCTA
    TACTCAGCAG GTACAGCACT GCTTGAACAG AATCTGCATA CCTTGGATAG CCTTCGAGTG
    TTCAATGATA AGAGCCCAGA AGAGGTGTTC ATGGAGATCA GGAGACCACT TGAATGGCAC
    TGCAAACAGA TGGATCATTT TGTTGGGCTA AATTTCAACT CCAACTTCAA CTTTGCACTA
    GTGGGGCACC TCCTGAAAGG GTACAGGCAT CCTTCACCTA CCACTGTAGC AAGAACAGTA
    AGGATTCTAC ATACACTACT AGCTCTGGTT AACAAACACA GGAACTGTGA CAAGTTTGAG
    GTGAACACCC AGAGCGTGGC TTACCTAGCA GCTTTGCTGA CAGTGTCTGA GGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGGAAGTCT CTCTTGCTGA CAGATGTTGC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATACATCAC AGTGACACTA CCTATAGGAC ACTAAAGGAA
    AATCAACCAT GGTCATCACC AAAGGGATCA GACAGACACC TGGCTGCCAG TTACCCGACA
    GTGGGACAGA TAAGCCCTCG AACACGAAAG TCCATGAGTT TGGATATGGG GCAGCCTTCA
    CAAGCGAACA CTAAAAAGCT TCTAGGTACA AGGAAAAGTT TTGACCATTT GATATCGGAC
    ACAAAGGCTC CTAAAAGGCA AGACATGGAA TCTGGAATCA CAACGCCTCC CAAAATGAGG
    AGAGTAGCCG AAAATGATTA TGAAATGGAA ACCCAGAGAA TATCACCGCA GCAGCACCCC
    CATCTCCGGA AAGTTTCTGT TTCTGAGTCA AATGTCCTCC TGGATGAAGA AGTTCTGACT
    GATCCAAAAA TTCAAGCACT GCTGCTTACA GTTCTTGCCA CGTTAGTGAA GTACACTACT
    GATGAATTTG ACCAGCGGAT TCTGTATGAG TATTTAGCAG AAGCCAGTGT TGTCTTCCCA
    AAGGTCTTTC CTGTTGTGCA TAATTTATTG GATTCCAAGA TCAATACCCT CTTGTCGCTG
    TGTCAAGATC CAAATTTGTT GAATCCAATT CATGGGATTG TTCAGAGTGT TGTCTACCAT
    GAGGAGTCTC CACCCCAATA CCAAACTTCT TATCTACAAA GTTTTGGATT TAATGGGTTG
    TGGAGGTTTG CTGGCCCATT TTCCAAGCAA ACACAAATCC CAGACTATGC TGAGCTCATA
    GTGAAGTTTC TTGATGCCTT GATTGACACG TATTTGCCTG GAATTGATGA GGAAACCAGT
    GAAGAATCTC TCCTGACACC CACGTCTCCA TATCCTCCCG CAGTGCAGAG CCAGCTTAGC
    ATTACAGCTA ACCTTAACCT TTCCAATTCC ATGACCTCGC TCGCAACTTC CCAGCACTCC
    CCAGCTTCTC TGCCTTGCTC TAAATCTGCA GTTTTCATGC AGCTTCCTCA TCAAGGGATC
    GACAAGGAGA ACGTGGAGCT CTCGCCCACC ACTGGCCACA CCAACAGCGG GAGGATGCGC
    CACGGCTCCG CCAGCCAGGT GCAAAAGCAG CGAAGTGCTG GCAGCTTGAA ACGTCACAGC
    ATTAAGAAGA TTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa44369
    Clone ID Related Accession (Same CDS sequence) XM_015295852.1 , XM_015295853.1
    Accession Version XM_015295853.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8598bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofibromin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455988.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
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    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
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    3601
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    3721
    3781
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    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
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    4621
    4681
    4741
    4801
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    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
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    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
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    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGATGCCAAC CAGTCACTGT TTTGCATGGC AGTGGGCCTC TGTTGCTAGT
    CTGACATTTA AAGAGAAAAT GACAAGCCTT AAATTTAAAG AAAAGCCTAC TGACTTGGAA
    ACCAAAAGCT ACAAGTTCCT GCTGTTATCC TTGGTAAAAC TGATCCATGC TGATCCAAAG
    CTTTTGCTGT GTAATCCAAG GAAGCAAGGG CCTGAGACAC AAGGCAGCAC TGCTGAATTA
    ATTACAGGCC TTGTACAACT CGTTCCACAA TCCAGCATGC CAGATATAGC ACAAGAAGCC
    ATGGAGGCCT TGCTAGTTCT TCACCAGTTA GACAGCATTG ACTTATGGAA TCCTGATGCA
    CCTATAGAAA CTTTCTGGGA AATTAGTTCA CAAATGCTTT TTTATATCTG CAAGAAATTA
    ACCAGTCATC AGATGCTCAG CAGTACAGAA ATCCTCAAGT GGCTGCGAGA GATTCTCATC
    TGTAGGAATA AATTTCTTCT AAAAAATAAA CAATCAGACA GAACTTCCTG TCACTTTCTC
    TTCCTTTATG ATGTCTCTGG AGGTGGAGCT AGTCAGATGT CCCTTGACCA CGAAGAGATG
    ATGCGCTGTG CTCCCGGAGC CACCCTCAGA AAAGGAAAAG GGAATTCTTC CATGGAAAGT
    ACAGCTGGAT GCAGTGGAAC ACCCATTTGT CGCCAGGCCC AAACAAAATT GGAAGTTGCC
    TTATACATGT TTTTGTGGAG TCCGGATATT GAGGCAGTCC TTGTTGCAAT GTCCTGTTTC
    CGTCACCTCT GTGAGGAAGC AGATATCAGA TGTGGAGTAG ATGAAGTTTC AGTTCATAAT
    TTTTTGCCAA ACTACAACAC TTTCATGGAG TTTGCATCTG TTAGCAATAT GATGTCAACA
    GGGAGAGCAG CTCTTCAGAA AAGAGTGATG GCTTTATTAA GACGTATTGA ACATCCAACT
    GCAGGGAACA CAGAGGCTTG GGAGGATACA CATGCAAAAT GGGAACAAGC TACAAAACTA
    ATCCTTAACT ATCCAAAGAC CAAAATGGAA GACGGGCAGG TTACTGAAAG TCTTCATAAG
    ACAATTGTTA AGAGACGAAT GTCACATGTT AGTGGGGGAG GCTCCATAGA CTTGTCTGAC
    ACAGATTCTC TTCAGGAATG GATCAACATG ACGGGATTCC TCTGTGCCCT CGGAGGAGTG
    TGCCTGCAGC ATCGTAGCAA TGCAGGATTA GCAACCTACA GTCCACCCAT GGGCCCAGTC
    AATGAGCGTA AGGGCTCCAT GATATCTATG GTATCCACTG AGGGAAATGC AGAGACTCCT
    GTTAGCAAAT TCATAGATCG ATTACTGACT CTGATGGTCT GTAACCATGA GAAAGTGGGA
    CTACAAATAC GGACTAATAT TAAAGATCTG GTGGGTTTGG AGTTGAGTCC AGCACTTTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGATTC TCAAGGACAG
    GTTTTGTTAA CTGAGACCAA CACACAGTTT GTAGAACAAA CCATTGCTAT AATGAAGAAC
    TTGCTTGACA ATCATACAGA AGGGAGCTCA GAACATCTTG GACAAGCTAG TATTGAGACA
    ATGATGCTAA ACCTGGTTAG GTATGTGCGT GTACTTGGAA ATTTGGTACA TGCAATTCAA
    ATAAAAACTA AACTCTGTCA GTTGGTGGAA GTAATGATGG AGCGGAGAGA TGACCTTTCA
    TTTTGCCAAG AAATGAAATT TAGGAACAAA ATGGTGGAAT ATCTGACAGA CTGGGTTATG
    GGAACATCTA ATCAAGCAAC AGATGAAGAT GTGAAGTGCT TGACAAGAGA TTTGGACCAA
    GCAAGTATGG AAGCAGTGGT CTCTCTTCTT GCTGGGCTTC CGTTACAGCC TGAAGAAGGA
    GATGGTGTTG AGTTGATGGA AGCCAAATCT CAGTTATTTC TTAAATATTT CACACTGTTT
    ATGAACCTTC TAAATGACTG TAGTGAAGTT GAAGATGAAA GTGCACAAAC AGGTGGCAGG
    AAACGTGGGA TGTCCCGAAG ACTGGCTTCG CTGCGGCACT GCACTGTTCT TGCTATGTCA
    AATTTACTAA ATGCAAATGT GGACAGTGGT CTTATGCATT CAATAGGCTT GGGCTATCAT
    AAAGACCTCC AGACAAGAGC TACATTTATG GAAGTCCTCA CCAAAATTCT ACAGCAGGGA
    ACAGAATTTG ATACGTTAGC AGAAACGGTG CTGGCAGATC GGTTTGAGAG GCTGGTGGAG
    CTAGTTACAA TGATGGGTGA TCAGGGGGAG CTTCCTATTG CTATGGCTTT AGCAAATGTG
    GTGCCTTGTT CTCAGTGGGA TGAGCTAGCT CGTGTTCTGG TCACACTCTT TGATTCCCGG
    CACTTGCTCT ACCAGCTACT TTGGAATATG TTTTCAAAGG AAGTTGAACT GGCAGATTCC
    ATGCAGACAC TCTTCCGAGG AAACAGCTTA GCTAGTAAAA TAATGACTTT CTGTTTTAAG
    GTATATGGTG CTACTTATTT GCAGAAGCTT TTGGAACCTT TATTAAGAAC AGTGATCACA
    TCCTCTGAAT GGCAGCATGT TAGCTTTGAG GTGGATCCTA CAAGACTGGA GCCTACAGAA
    AGCCTTGAAG AGAACCAGCG GAGTCTCTTG CAAATGACAG AAAAATTTTT TCATGCAATC
    ATTAATTCTT CTTCGGAATT CCCACCACAA CTTCGCAGTG TGTGCCATTG TTTGTACCAG
    GCAACTTGCC ACTCTCTACT GAATAAAGCT ACCGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTTGTTA GTCAGCGATT TCCTCAGAAC AGCATTGGAG CAGTTGGGAG TGCCATGTTC
    CTGCGGTTCA TCAACCCTGC GATCGTCTCC CCGTACGAGG CAGGGATTTT AGATAAAAAG
    CCTCCGCCCA GAATTGAGAG GGGACTGAAA CTGATGTCAA AGATACTTCA GAGTATTGCC
    AACCACGTCC TGTTTACAAA AGAAGAACAT ATGAGGCCTT TTAATGACTT CGTAAAAAGC
    AATTTTGATG CAGCTCGAAG GTTTTTCCTT GATATTGCAT CTGATTGCCC TGCGAGTGAC
    ACTGTCAATC ACAGCCTGTC TTTCATCAGT GATGGCAATG TTCTTGCTTT GCACCGGTTG
    TTGTGGAACA ATCAGGAGAA AATTGGCCAG TACCTTTCCA GTAATAGGGA CCACAAAGCT
    GTTGGAAGGC GACCTTTTGA CAAAATGGCT ACTCTTCTTG CCTATCTGGG TCCACCAGAG
    CACAAACCTG TGGCAGATAC ACATTGGTCC AGTTTAAACC TCACCAGTTC GAAATTTGAA
    GAATTTATGA CAAGGCATCA GGTACATGAA AAAGAAGAGT TCAAAGCATT GAAAACGTTA
    AATATTTTCT ACCAGGCTGG GACATCCAAA GCTGGCAATC CTGTTTTCTA CTACATTGCT
    CGGCGTTTCA AGACTGGTCA GATCAATGGA GATTTATTGA TATACCACGT CCTGCTAACT
    CTGAAGCCAT ACTACGCAAA GCCATATGAA ATTGTAGTGG ACCTCACACA TGCTGGCCCC
    AGTAATCGCT TTAAAACAGA CTTTCTTTCT AAATGGTTTG TAGTTTTTCC AGGCTTTGCT
    TATGAAAATG TCTCAGCAGT TTTTATTTAT AACTGTAATT CTTGGGTCAG AGAATACACC
    AAATACCACG AAAGACTCTT GACAGGTTTG AAAGGAAGCA AAAGGCTTAT TTTCATAGAC
    TCTCCAGGAA AACTGGCGGA GCACATTGAA CATGACCAGC AGAAACTTCC AGCAGCTACC
    TTAGCTTTGG AAGAGGACTT GAAAGTATTT CACAATGCCC TCAAATTGGC TCACAAAGAC
    ACCAAAGTGT CTATTAAAGT TGGATCAACA GCTGTGCAGG TGACATCAGC AGAACGAACA
    AGAGTCCTGG GACAGACAGT GTTTTTAAAT GATATATACT ACGCCTCTGA AATTGAGGAA
    ATATGTCTTG TTGATGAGAA TCAGTTTACA TTGACGATTG CCAACCAGGG AACGCCACTC
    ACCTTCATGC ATCAGGAGTG TGAAGCCATT GTGAGGTCCA TTATTCATAT ACGGACACGG
    TGGGAGCTGT CGCAACCAGA CTCCATCCCA CAGCACACTA AAATTCGCCC TAAGGATGTT
    CCCGGAACAC TACTGAACAT AGCATTGCTC AACCTGGGAA GCTCAGACCC GAGTTTGCGG
    TCTGCTGCCT ATAATCTTCT GTGTGCCTTA ACCTGTACCT TTAATTTAAA GATTGAGGGC
    CAGTTACTGG AAACTTCAGG TCTGTGTATT CCTGCCAACA ACACGCTCTT TATTGTGTCT
    ATTAGTAAGA CTCTTGCAGC TAATGAGCCA CACCTTACCC TAGAGTTCTT GGAAGAGTGT
    ATTTCAGGAT TTAGCAAATC TAGTATTGAA CTGAAACATC TTTGTCTGGA ATATATGACT
    CCCTGGCTGT CCAATCTGGT CCGATTCTGC AAACACAACG ACGATGCGAA GCGCCAGCGA
    GTCACTGCCA TTCTTGATAA GCTTATAACA ATGACAATAA ATGAAAAACA GATGTACCCT
    TCTATTCAGG CTAAAATATG GGGAAGTCTT GGACAGATTA CAGATCTCCT TGACGTAGTG
    CTGGACAGTT TCATCAAAAC TAGTGCCACG GGGGGCTTAG GATCCATTAA AGCTGAGGTT
    ATGGCAGACA CAGCAGTAGC GCTTGCTTCT GGAAATGTAA AATTGGTTTC AAGCAAAGTG
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACTTGTCTGT CTCCAACTCC TACACTGGAA
    CAGCACCTGA TGTGGGATGA TATTGCCATT CTAGCACGTT ACATGCTGAT GCTCTCCTTC
    AACAATTCTC TGGATGTGGC TGCACATCTC CCCTACCTCT TTCATGTGGT TACTTTGTTG
    GTGGCCACTG GACCCCTTTC TCTCAGAGCT TCCACTCATG GTCTTGTCAT CAACATCATT
    CATTCTCTGT GCACTTGCTC ACAGCTTCAT TTTAGTGAGG AGACCAAGCA AGTTTTAAGA
    CTGAGCTTGA CTGAATTTTC TCTGCCTAAA TTTTATTTGC TGTTTGGAAT CAGCAAAGTG
    AAATCAGCTG CAGTCATAGC ATTCCGCTCC AGCTATCGAG ACAGGTCATT TTCACCTGGC
    TCCTACGAGA GGGAGACTTT TGCACTGACC TCCTTGGAAA CTGTTACTGA AGCTTTGCTG
    GAGATCATGG AGGCTTGTAT GAGAGATATT CCAACATGCA AGTGGCTGGA CCAGTGGACC
    GAACTAGCAC AAAAGTTTGC ATTCCAGTAC AATCCATCCC TGCAGCCAAG AGCACTCGTT
    GTCTTTGGCT GTATAAGTAA GCGTGTCTCT CATGGACAAA TAAAACAAAT AATCCGAATT
    CTTAGCAAGG GGCTTGAGAG CTGTTTAAAA GGCCCTGATA ATTACAATAG CCAAGTTCTA
    ATAGAAGCTA CGGTTATAGC ACTCACCAAA CTGCAGCCTC TTCTTAACAA GGACTCCCCC
    ATGCACAAGG CCCTCTTCTG GGTAGCCATG GCAGTACTGC AGTTGGATGA AGTCAACCTA
    TACTCAGCAG GTACAGCACT GCTTGAACAG AATCTGCATA CCTTGGATAG CCTTCGAGTG
    TTCAATGATA AGAGCCCAGA AGAGGTGTTC ATGGAGATCA GGAGACCACT TGAATGGCAC
    TGCAAACAGA TGGATCATTT TGTTGGGCTA AATTTCAACT CCAACTTCAA CTTTGCACTA
    GTGGGGCACC TCCTGAAAGG GTACAGGCAT CCTTCACCTA CCACTGTAGC AAGAACAGTA
    AGGATTCTAC ATACACTACT AGCTCTGGTT AACAAACACA GGAACTGTGA CAAGTTTGAG
    GTGAACACCC AGAGCGTGGC TTACCTAGCA GCTTTGCTGA CAGTGTCTGA GGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGGAAGTCT CTCTTGCTGA CAGATGTTGC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATACATCAC AGTGACACTA CCTATAGGAC ACTAAAGGAA
    AATCAACCAT GGTCATCACC AAAGGGATCA GACAGACACC TGGCTGCCAG TTACCCGACA
    GTGGGACAGA TAAGCCCTCG AACACGAAAG TCCATGAGTT TGGATATGGG GCAGCCTTCA
    CAAGCGAACA CTAAAAAGCT TCTAGGTACA AGGAAAAGTT TTGACCATTT GATATCGGAC
    ACAAAGGCTC CTAAAAGGCA AGACATGGAA TCTGGAATCA CAACGCCTCC CAAAATGAGG
    AGAGTAGCCG AAAATGATTA TGAAATGGAA ACCCAGAGAA TATCACCGCA GCAGCACCCC
    CATCTCCGGA AAGTTTCTGT TTCTGAGTCA AATGTCCTCC TGGATGAAGA AGTTCTGACT
    GATCCAAAAA TTCAAGCACT GCTGCTTACA GTTCTTGCCA CGTTAGTGAA GTACACTACT
    GATGAATTTG ACCAGCGGAT TCTGTATGAG TATTTAGCAG AAGCCAGTGT TGTCTTCCCA
    AAGGTCTTTC CTGTTGTGCA TAATTTATTG GATTCCAAGA TCAATACCCT CTTGTCGCTG
    TGTCAAGATC CAAATTTGTT GAATCCAATT CATGGGATTG TTCAGAGTGT TGTCTACCAT
    GAGGAGTCTC CACCCCAATA CCAAACTTCT TATCTACAAA GTTTTGGATT TAATGGGTTG
    TGGAGGTTTG CTGGCCCATT TTCCAAGCAA ACACAAATCC CAGACTATGC TGAGCTCATA
    GTGAAGTTTC TTGATGCCTT GATTGACACG TATTTGCCTG GAATTGATGA GGAAACCAGT
    GAAGAATCTC TCCTGACACC CACGTCTCCA TATCCTCCCG CAGTGCAGAG CCAGCTTAGC
    ATTACAGCTA ACCTTAACCT TTCCAATTCC ATGACCTCGC TCGCAACTTC CCAGCACTCC
    CCAGCTTCTC TGCCTTGCTC TAAATCTGCA GTTTTCATGC AGCTTCCTCA TCAAGGGATC
    GACAAGGAGA ACGTGGAGCT CTCGCCCACC ACTGGCCACA CCAACAGCGG GAGGATGCGC
    CACGGCTCCG CCAGCCAGGT GCAAAAGCAG CGAAGTGCTG GCAGCTTGAA ACGTCACAGC
    ATTAAGAAGA TTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015151339.1
    CDS217..8814
    Translation

    Target ORF information:

    RefSeq Version XM_015295853.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofibromin 1 (NF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015295853.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGATGCCAAC CAGTCACTGT TTTGCATGGC AGTGGGCCTC TGTTGCTAGT
    CTGACATTTA AAGAGAAAAT GACAAGCCTT AAATTTAAAG AAAAGCCTAC TGACTTGGAA
    ACCAAAAGCT ACAAGTTCCT GCTGTTATCC TTGGTAAAAC TGATCCATGC TGATCCAAAG
    CTTTTGCTGT GTAATCCAAG GAAGCAAGGG CCTGAGACAC AAGGCAGCAC TGCTGAATTA
    ATTACAGGCC TTGTACAACT CGTTCCACAA TCCAGCATGC CAGATATAGC ACAAGAAGCC
    ATGGAGGCCT TGCTAGTTCT TCACCAGTTA GACAGCATTG ACTTATGGAA TCCTGATGCA
    CCTATAGAAA CTTTCTGGGA AATTAGTTCA CAAATGCTTT TTTATATCTG CAAGAAATTA
    ACCAGTCATC AGATGCTCAG CAGTACAGAA ATCCTCAAGT GGCTGCGAGA GATTCTCATC
    TGTAGGAATA AATTTCTTCT AAAAAATAAA CAATCAGACA GAACTTCCTG TCACTTTCTC
    TTCCTTTATG ATGTCTCTGG AGGTGGAGCT AGTCAGATGT CCCTTGACCA CGAAGAGATG
    ATGCGCTGTG CTCCCGGAGC CACCCTCAGA AAAGGAAAAG GGAATTCTTC CATGGAAAGT
    ACAGCTGGAT GCAGTGGAAC ACCCATTTGT CGCCAGGCCC AAACAAAATT GGAAGTTGCC
    TTATACATGT TTTTGTGGAG TCCGGATATT GAGGCAGTCC TTGTTGCAAT GTCCTGTTTC
    CGTCACCTCT GTGAGGAAGC AGATATCAGA TGTGGAGTAG ATGAAGTTTC AGTTCATAAT
    TTTTTGCCAA ACTACAACAC TTTCATGGAG TTTGCATCTG TTAGCAATAT GATGTCAACA
    GGGAGAGCAG CTCTTCAGAA AAGAGTGATG GCTTTATTAA GACGTATTGA ACATCCAACT
    GCAGGGAACA CAGAGGCTTG GGAGGATACA CATGCAAAAT GGGAACAAGC TACAAAACTA
    ATCCTTAACT ATCCAAAGAC CAAAATGGAA GACGGGCAGG TTACTGAAAG TCTTCATAAG
    ACAATTGTTA AGAGACGAAT GTCACATGTT AGTGGGGGAG GCTCCATAGA CTTGTCTGAC
    ACAGATTCTC TTCAGGAATG GATCAACATG ACGGGATTCC TCTGTGCCCT CGGAGGAGTG
    TGCCTGCAGC ATCGTAGCAA TGCAGGATTA GCAACCTACA GTCCACCCAT GGGCCCAGTC
    AATGAGCGTA AGGGCTCCAT GATATCTATG GTATCCACTG AGGGAAATGC AGAGACTCCT
    GTTAGCAAAT TCATAGATCG ATTACTGACT CTGATGGTCT GTAACCATGA GAAAGTGGGA
    CTACAAATAC GGACTAATAT TAAAGATCTG GTGGGTTTGG AGTTGAGTCC AGCACTTTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGATTC TCAAGGACAG
    GTTTTGTTAA CTGAGACCAA CACACAGTTT GTAGAACAAA CCATTGCTAT AATGAAGAAC
    TTGCTTGACA ATCATACAGA AGGGAGCTCA GAACATCTTG GACAAGCTAG TATTGAGACA
    ATGATGCTAA ACCTGGTTAG GTATGTGCGT GTACTTGGAA ATTTGGTACA TGCAATTCAA
    ATAAAAACTA AACTCTGTCA GTTGGTGGAA GTAATGATGG AGCGGAGAGA TGACCTTTCA
    TTTTGCCAAG AAATGAAATT TAGGAACAAA ATGGTGGAAT ATCTGACAGA CTGGGTTATG
    GGAACATCTA ATCAAGCAAC AGATGAAGAT GTGAAGTGCT TGACAAGAGA TTTGGACCAA
    GCAAGTATGG AAGCAGTGGT CTCTCTTCTT GCTGGGCTTC CGTTACAGCC TGAAGAAGGA
    GATGGTGTTG AGTTGATGGA AGCCAAATCT CAGTTATTTC TTAAATATTT CACACTGTTT
    ATGAACCTTC TAAATGACTG TAGTGAAGTT GAAGATGAAA GTGCACAAAC AGGTGGCAGG
    AAACGTGGGA TGTCCCGAAG ACTGGCTTCG CTGCGGCACT GCACTGTTCT TGCTATGTCA
    AATTTACTAA ATGCAAATGT GGACAGTGGT CTTATGCATT CAATAGGCTT GGGCTATCAT
    AAAGACCTCC AGACAAGAGC TACATTTATG GAAGTCCTCA CCAAAATTCT ACAGCAGGGA
    ACAGAATTTG ATACGTTAGC AGAAACGGTG CTGGCAGATC GGTTTGAGAG GCTGGTGGAG
    CTAGTTACAA TGATGGGTGA TCAGGGGGAG CTTCCTATTG CTATGGCTTT AGCAAATGTG
    GTGCCTTGTT CTCAGTGGGA TGAGCTAGCT CGTGTTCTGG TCACACTCTT TGATTCCCGG
    CACTTGCTCT ACCAGCTACT TTGGAATATG TTTTCAAAGG AAGTTGAACT GGCAGATTCC
    ATGCAGACAC TCTTCCGAGG AAACAGCTTA GCTAGTAAAA TAATGACTTT CTGTTTTAAG
    GTATATGGTG CTACTTATTT GCAGAAGCTT TTGGAACCTT TATTAAGAAC AGTGATCACA
    TCCTCTGAAT GGCAGCATGT TAGCTTTGAG GTGGATCCTA CAAGACTGGA GCCTACAGAA
    AGCCTTGAAG AGAACCAGCG GAGTCTCTTG CAAATGACAG AAAAATTTTT TCATGCAATC
    ATTAATTCTT CTTCGGAATT CCCACCACAA CTTCGCAGTG TGTGCCATTG TTTGTACCAG
    GCAACTTGCC ACTCTCTACT GAATAAAGCT ACCGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTTGTTA GTCAGCGATT TCCTCAGAAC AGCATTGGAG CAGTTGGGAG TGCCATGTTC
    CTGCGGTTCA TCAACCCTGC GATCGTCTCC CCGTACGAGG CAGGGATTTT AGATAAAAAG
    CCTCCGCCCA GAATTGAGAG GGGACTGAAA CTGATGTCAA AGATACTTCA GAGTATTGCC
    AACCACGTCC TGTTTACAAA AGAAGAACAT ATGAGGCCTT TTAATGACTT CGTAAAAAGC
    AATTTTGATG CAGCTCGAAG GTTTTTCCTT GATATTGCAT CTGATTGCCC TGCGAGTGAC
    ACTGTCAATC ACAGCCTGTC TTTCATCAGT GATGGCAATG TTCTTGCTTT GCACCGGTTG
    TTGTGGAACA ATCAGGAGAA AATTGGCCAG TACCTTTCCA GTAATAGGGA CCACAAAGCT
    GTTGGAAGGC GACCTTTTGA CAAAATGGCT ACTCTTCTTG CCTATCTGGG TCCACCAGAG
    CACAAACCTG TGGCAGATAC ACATTGGTCC AGTTTAAACC TCACCAGTTC GAAATTTGAA
    GAATTTATGA CAAGGCATCA GGTACATGAA AAAGAAGAGT TCAAAGCATT GAAAACGTTA
    AATATTTTCT ACCAGGCTGG GACATCCAAA GCTGGCAATC CTGTTTTCTA CTACATTGCT
    CGGCGTTTCA AGACTGGTCA GATCAATGGA GATTTATTGA TATACCACGT CCTGCTAACT
    CTGAAGCCAT ACTACGCAAA GCCATATGAA ATTGTAGTGG ACCTCACACA TGCTGGCCCC
    AGTAATCGCT TTAAAACAGA CTTTCTTTCT AAATGGTTTG TAGTTTTTCC AGGCTTTGCT
    TATGAAAATG TCTCAGCAGT TTTTATTTAT AACTGTAATT CTTGGGTCAG AGAATACACC
    AAATACCACG AAAGACTCTT GACAGGTTTG AAAGGAAGCA AAAGGCTTAT TTTCATAGAC
    TCTCCAGGAA AACTGGCGGA GCACATTGAA CATGACCAGC AGAAACTTCC AGCAGCTACC
    TTAGCTTTGG AAGAGGACTT GAAAGTATTT CACAATGCCC TCAAATTGGC TCACAAAGAC
    ACCAAAGTGT CTATTAAAGT TGGATCAACA GCTGTGCAGG TGACATCAGC AGAACGAACA
    AGAGTCCTGG GACAGACAGT GTTTTTAAAT GATATATACT ACGCCTCTGA AATTGAGGAA
    ATATGTCTTG TTGATGAGAA TCAGTTTACA TTGACGATTG CCAACCAGGG AACGCCACTC
    ACCTTCATGC ATCAGGAGTG TGAAGCCATT GTGAGGTCCA TTATTCATAT ACGGACACGG
    TGGGAGCTGT CGCAACCAGA CTCCATCCCA CAGCACACTA AAATTCGCCC TAAGGATGTT
    CCCGGAACAC TACTGAACAT AGCATTGCTC AACCTGGGAA GCTCAGACCC GAGTTTGCGG
    TCTGCTGCCT ATAATCTTCT GTGTGCCTTA ACCTGTACCT TTAATTTAAA GATTGAGGGC
    CAGTTACTGG AAACTTCAGG TCTGTGTATT CCTGCCAACA ACACGCTCTT TATTGTGTCT
    ATTAGTAAGA CTCTTGCAGC TAATGAGCCA CACCTTACCC TAGAGTTCTT GGAAGAGTGT
    ATTTCAGGAT TTAGCAAATC TAGTATTGAA CTGAAACATC TTTGTCTGGA ATATATGACT
    CCCTGGCTGT CCAATCTGGT CCGATTCTGC AAACACAACG ACGATGCGAA GCGCCAGCGA
    GTCACTGCCA TTCTTGATAA GCTTATAACA ATGACAATAA ATGAAAAACA GATGTACCCT
    TCTATTCAGG CTAAAATATG GGGAAGTCTT GGACAGATTA CAGATCTCCT TGACGTAGTG
    CTGGACAGTT TCATCAAAAC TAGTGCCACG GGGGGCTTAG GATCCATTAA AGCTGAGGTT
    ATGGCAGACA CAGCAGTAGC GCTTGCTTCT GGAAATGTAA AATTGGTTTC AAGCAAAGTG
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACTTGTCTGT CTCCAACTCC TACACTGGAA
    CAGCACCTGA TGTGGGATGA TATTGCCATT CTAGCACGTT ACATGCTGAT GCTCTCCTTC
    AACAATTCTC TGGATGTGGC TGCACATCTC CCCTACCTCT TTCATGTGGT TACTTTGTTG
    GTGGCCACTG GACCCCTTTC TCTCAGAGCT TCCACTCATG GTCTTGTCAT CAACATCATT
    CATTCTCTGT GCACTTGCTC ACAGCTTCAT TTTAGTGAGG AGACCAAGCA AGTTTTAAGA
    CTGAGCTTGA CTGAATTTTC TCTGCCTAAA TTTTATTTGC TGTTTGGAAT CAGCAAAGTG
    AAATCAGCTG CAGTCATAGC ATTCCGCTCC AGCTATCGAG ACAGGTCATT TTCACCTGGC
    TCCTACGAGA GGGAGACTTT TGCACTGACC TCCTTGGAAA CTGTTACTGA AGCTTTGCTG
    GAGATCATGG AGGCTTGTAT GAGAGATATT CCAACATGCA AGTGGCTGGA CCAGTGGACC
    GAACTAGCAC AAAAGTTTGC ATTCCAGTAC AATCCATCCC TGCAGCCAAG AGCACTCGTT
    GTCTTTGGCT GTATAAGTAA GCGTGTCTCT CATGGACAAA TAAAACAAAT AATCCGAATT
    CTTAGCAAGG GGCTTGAGAG CTGTTTAAAA GGCCCTGATA ATTACAATAG CCAAGTTCTA
    ATAGAAGCTA CGGTTATAGC ACTCACCAAA CTGCAGCCTC TTCTTAACAA GGACTCCCCC
    ATGCACAAGG CCCTCTTCTG GGTAGCCATG GCAGTACTGC AGTTGGATGA AGTCAACCTA
    TACTCAGCAG GTACAGCACT GCTTGAACAG AATCTGCATA CCTTGGATAG CCTTCGAGTG
    TTCAATGATA AGAGCCCAGA AGAGGTGTTC ATGGAGATCA GGAGACCACT TGAATGGCAC
    TGCAAACAGA TGGATCATTT TGTTGGGCTA AATTTCAACT CCAACTTCAA CTTTGCACTA
    GTGGGGCACC TCCTGAAAGG GTACAGGCAT CCTTCACCTA CCACTGTAGC AAGAACAGTA
    AGGATTCTAC ATACACTACT AGCTCTGGTT AACAAACACA GGAACTGTGA CAAGTTTGAG
    GTGAACACCC AGAGCGTGGC TTACCTAGCA GCTTTGCTGA CAGTGTCTGA GGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGGAAGTCT CTCTTGCTGA CAGATGTTGC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATACATCAC AGTGACACTA CCTATAGGAC ACTAAAGGAA
    AATCAACCAT GGTCATCACC AAAGGGATCA GACAGACACC TGGCTGCCAG TTACCCGACA
    GTGGGACAGA TAAGCCCTCG AACACGAAAG TCCATGAGTT TGGATATGGG GCAGCCTTCA
    CAAGCGAACA CTAAAAAGCT TCTAGGTACA AGGAAAAGTT TTGACCATTT GATATCGGAC
    ACAAAGGCTC CTAAAAGGCA AGACATGGAA TCTGGAATCA CAACGCCTCC CAAAATGAGG
    AGAGTAGCCG AAAATGATTA TGAAATGGAA ACCCAGAGAA TATCACCGCA GCAGCACCCC
    CATCTCCGGA AAGTTTCTGT TTCTGAGTCA AATGTCCTCC TGGATGAAGA AGTTCTGACT
    GATCCAAAAA TTCAAGCACT GCTGCTTACA GTTCTTGCCA CGTTAGTGAA GTACACTACT
    GATGAATTTG ACCAGCGGAT TCTGTATGAG TATTTAGCAG AAGCCAGTGT TGTCTTCCCA
    AAGGTCTTTC CTGTTGTGCA TAATTTATTG GATTCCAAGA TCAATACCCT CTTGTCGCTG
    TGTCAAGATC CAAATTTGTT GAATCCAATT CATGGGATTG TTCAGAGTGT TGTCTACCAT
    GAGGAGTCTC CACCCCAATA CCAAACTTCT TATCTACAAA GTTTTGGATT TAATGGGTTG
    TGGAGGTTTG CTGGCCCATT TTCCAAGCAA ACACAAATCC CAGACTATGC TGAGCTCATA
    GTGAAGTTTC TTGATGCCTT GATTGACACG TATTTGCCTG GAATTGATGA GGAAACCAGT
    GAAGAATCTC TCCTGACACC CACGTCTCCA TATCCTCCCG CAGTGCAGAG CCAGCTTAGC
    ATTACAGCTA ACCTTAACCT TTCCAATTCC ATGACCTCGC TCGCAACTTC CCAGCACTCC
    CCAGCTTCTC TGCCTTGCTC TAAATCTGCA GTTTTCATGC AGCTTCCTCA TCAAGGGATC
    GACAAGGAGA ACGTGGAGCT CTCGCCCACC ACTGGCCACA CCAACAGCGG GAGGATGCGC
    CACGGCTCCG CCAGCCAGGT GCAAAAGCAG CGAAGTGCTG GCAGCTTGAA ACGTCACAGC
    ATTAAGAAGA TTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa44370
    Clone ID Related Accession (Same CDS sequence) XM_015295854.1
    Accession Version XM_015295854.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofibromin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455988.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGAGTCTGAC ATTTAAAGAG AAAATGACAA GCCTTAAATT TAAAGAAAAG
    CCTACTGACT TGGAAACCAA AAGCTACAAG TTCCTGCTGT TATCCTTGGT AAAACTGATC
    CATGCTGATC CAAAGCTTTT GCTGTGTAAT CCAAGGAAGC AAGGGCCTGA GACACAAGGC
    AGCACTGCTG AATTAATTAC AGGCCTTGTA CAACTCGTTC CACAATCCAG CATGCCAGAT
    ATAGCACAAG AAGCCATGGA GGCCTTGCTA GTTCTTCACC AGTTAGACAG CATTGACTTA
    TGGAATCCTG ATGCACCTAT AGAAACTTTC TGGGAAATTA GTTCACAAAT GCTTTTTTAT
    ATCTGCAAGA AATTAACCAG TCATCAGATG CTCAGCAGTA CAGAAATCCT CAAGTGGCTG
    CGAGAGATTC TCATCTGTAG GAATAAATTT CTTCTAAAAA ATAAACAATC AGACAGAACT
    TCCTGTCACT TTCTCTTCCT TTATGATGTC TCTGGAGGTG GAGCTAGTCA GATGTCCCTT
    GACCACGAAG AGATGATGCG CTGTGCTCCC GGAGCCACCC TCAGAAAAGG AAAAGGGAAT
    TCTTCCATGG AAAGTACAGC TGGATGCAGT GGAACACCCA TTTGTCGCCA GGCCCAAACA
    AAATTGGAAG TTGCCTTATA CATGTTTTTG TGGAGTCCGG ATATTGAGGC AGTCCTTGTT
    GCAATGTCCT GTTTCCGTCA CCTCTGTGAG GAAGCAGATA TCAGATGTGG AGTAGATGAA
    GTTTCAGTTC ATAATTTTTT GCCAAACTAC AACACTTTCA TGGAGTTTGC ATCTGTTAGC
    AATATGATGT CAACAGGGAG AGCAGCTCTT CAGAAAAGAG TGATGGCTTT ATTAAGACGT
    ATTGAACATC CAACTGCAGG GAACACAGAG GCTTGGGAGG ATACACATGC AAAATGGGAA
    CAAGCTACAA AACTAATCCT TAACTATCCA AAGACCAAAA TGGAAGACGG GCAGGTTACT
    GAAAGTCTTC ATAAGACAAT TGTTAAGAGA CGAATGTCAC ATGTTAGTGG GGGAGGCTCC
    ATAGACTTGT CTGACACAGA TTCTCTTCAG GAATGGATCA ACATGACGGG ATTCCTCTGT
    GCCCTCGGAG GAGTGTGCCT GCAGCATCGT AGCAATGCAG GATTAGCAAC CTACAGTCCA
    CCCATGGGCC CAGTCAATGA GCGTAAGGGC TCCATGATAT CTATGGTATC CACTGAGGGA
    AATGCAGAGA CTCCTGTTAG CAAATTCATA GATCGATTAC TGACTCTGAT GGTCTGTAAC
    CATGAGAAAG TGGGACTACA AATACGGACT AATATTAAAG ATCTGGTGGG TTTGGAGTTG
    AGTCCAGCAC TTTATCCAAT GCTATTTAAC AAATTGAAGA ATACCATCAG CAAGTTTTTT
    GATTCTCAAG GACAGGTTTT GTTAACTGAG ACCAACACAC AGTTTGTAGA ACAAACCATT
    GCTATAATGA AGAACTTGCT TGACAATCAT ACAGAAGGGA GCTCAGAACA TCTTGGACAA
    GCTAGTATTG AGACAATGAT GCTAAACCTG GTTAGGTATG TGCGTGTACT TGGAAATTTG
    GTACATGCAA TTCAAATAAA AACTAAACTC TGTCAGTTGG TGGAAGTAAT GATGGAGCGG
    AGAGATGACC TTTCATTTTG CCAAGAAATG AAATTTAGGA ACAAAATGGT GGAATATCTG
    ACAGACTGGG TTATGGGAAC ATCTAATCAA GCAACAGATG AAGATGTGAA GTGCTTGACA
    AGAGATTTGG ACCAAGCAAG TATGGAAGCA GTGGTCTCTC TTCTTGCTGG GCTTCCGTTA
    CAGCCTGAAG AAGGAGATGG TGTTGAGTTG ATGGAAGCCA AATCTCAGTT ATTTCTTAAA
    TATTTCACAC TGTTTATGAA CCTTCTAAAT GACTGTAGTG AAGTTGAAGA TGAAAGTGCA
    CAAACAGGTG GCAGGAAACG TGGGATGTCC CGAAGACTGG CTTCGCTGCG GCACTGCACT
    GTTCTTGCTA TGTCAAATTT ACTAAATGCA AATGTGGACA GTGGTCTTAT GCATTCAATA
    GGCTTGGGCT ATCATAAAGA CCTCCAGACA AGAGCTACAT TTATGGAAGT CCTCACCAAA
    ATTCTACAGC AGGGAACAGA ATTTGATACG TTAGCAGAAA CGGTGCTGGC AGATCGGTTT
    GAGAGGCTGG TGGAGCTAGT TACAATGATG GGTGATCAGG GGGAGCTTCC TATTGCTATG
    GCTTTAGCAA ATGTGGTGCC TTGTTCTCAG TGGGATGAGC TAGCTCGTGT TCTGGTCACA
    CTCTTTGATT CCCGGCACTT GCTCTACCAG CTACTTTGGA ATATGTTTTC AAAGGAAGTT
    GAACTGGCAG ATTCCATGCA GACACTCTTC CGAGGAAACA GCTTAGCTAG TAAAATAATG
    ACTTTCTGTT TTAAGGTATA TGGTGCTACT TATTTGCAGA AGCTTTTGGA ACCTTTATTA
    AGAACAGTGA TCACATCCTC TGAATGGCAG CATGTTAGCT TTGAGGTGGA TCCTACAAGA
    CTGGAGCCTA CAGAAAGCCT TGAAGAGAAC CAGCGGAGTC TCTTGCAAAT GACAGAAAAA
    TTTTTTCATG CAATCATTAA TTCTTCTTCG GAATTCCCAC CACAACTTCG CAGTGTGTGC
    CATTGTTTGT ACCAGGCAAC TTGCCACTCT CTACTGAATA AAGCTACCGT AAAAGAAAAA
    AAGGAAAACA AAAAATCAGT TGTTAGTCAG CGATTTCCTC AGAACAGCAT TGGAGCAGTT
    GGGAGTGCCA TGTTCCTGCG GTTCATCAAC CCTGCGATCG TCTCCCCGTA CGAGGCAGGG
    ATTTTAGATA AAAAGCCTCC GCCCAGAATT GAGAGGGGAC TGAAACTGAT GTCAAAGATA
    CTTCAGAGTA TTGCCAACCA CGTCCTGTTT ACAAAAGAAG AACATATGAG GCCTTTTAAT
    GACTTCGTAA AAAGCAATTT TGATGCAGCT CGAAGGTTTT TCCTTGATAT TGCATCTGAT
    TGCCCTGCGA GTGACACTGT CAATCACAGC CTGTCTTTCA TCAGTGATGG CAATGTTCTT
    GCTTTGCACC GGTTGTTGTG GAACAATCAG GAGAAAATTG GCCAGTACCT TTCCAGTAAT
    AGGGACCACA AAGCTGTTGG AAGGCGACCT TTTGACAAAA TGGCTACTCT TCTTGCCTAT
    CTGGGTCCAC CAGAGCACAA ACCTGTGGCA GATACACATT GGTCCAGTTT AAACCTCACC
    AGTTCGAAAT TTGAAGAATT TATGACAAGG CATCAGGTAC ATGAAAAAGA AGAGTTCAAA
    GCATTGAAAA CGTTAAATAT TTTCTACCAG GCTGGGACAT CCAAAGCTGG CAATCCTGTT
    TTCTACTACA TTGCTCGGCG TTTCAAGACT GGTCAGATCA ATGGAGATTT ATTGATATAC
    CACGTCCTGC TAACTCTGAA GCCATACTAC GCAAAGCCAT ATGAAATTGT AGTGGACCTC
    ACACATGCTG GCCCCAGTAA TCGCTTTAAA ACAGACTTTC TTTCTAAATG GTTTGTAGTT
    TTTCCAGGCT TTGCTTATGA AAATGTCTCA GCAGTTTTTA TTTATAACTG TAATTCTTGG
    GTCAGAGAAT ACACCAAATA CCACGAAAGA CTCTTGACAG GTTTGAAAGG AAGCAAAAGG
    CTTATTTTCA TAGACTCTCC AGGAAAACTG GCGGAGCACA TTGAACATGA CCAGCAGAAA
    CTTCCAGCAG CTACCTTAGC TTTGGAAGAG GACTTGAAAG TATTTCACAA TGCCCTCAAA
    TTGGCTCACA AAGACACCAA AGTGTCTATT AAAGTTGGAT CAACAGCTGT GCAGGTGACA
    TCAGCAGAAC GAACAAGAGT CCTGGGACAG ACAGTGTTTT TAAATGATAT ATACTACGCC
    TCTGAAATTG AGGAAATATG TCTTGTTGAT GAGAATCAGT TTACATTGAC GATTGCCAAC
    CAGGGAACGC CACTCACCTT CATGCATCAG GAGTGTGAAG CCATTGTGAG GTCCATTATT
    CATATACGGA CACGGTGGGA GCTGTCGCAA CCAGACTCCA TCCCACAGCA CACTAAAATT
    CGCCCTAAGG ATGTTCCCGG AACACTACTG AACATAGCAT TGCTCAACCT GGGAAGCTCA
    GACCCGAGTT TGCGGTCTGC TGCCTATAAT CTTCTGTGTG CCTTAACCTG TACCTTTAAT
    TTAAAGATTG AGGGCCAGTT ACTGGAAACT TCAGGTCTGT GTATTCCTGC CAACAACACG
    CTCTTTATTG TGTCTATTAG TAAGACTCTT GCAGCTAATG AGCCACACCT TACCCTAGAG
    TTCTTGGAAG AGTGTATTTC AGGATTTAGC AAATCTAGTA TTGAACTGAA ACATCTTTGT
    CTGGAATATA TGACTCCCTG GCTGTCCAAT CTGGTCCGAT TCTGCAAACA CAACGACGAT
    GCGAAGCGCC AGCGAGTCAC TGCCATTCTT GATAAGCTTA TAACAATGAC AATAAATGAA
    AAACAGATGT ACCCTTCTAT TCAGGCTAAA ATATGGGGAA GTCTTGGACA GATTACAGAT
    CTCCTTGACG TAGTGCTGGA CAGTTTCATC AAAACTAGTG CCACGGGGGG CTTAGGATCC
    ATTAAAGCTG AGGTTATGGC AGACACAGCA GTAGCGCTTG CTTCTGGAAA TGTAAAATTG
    GTTTCAAGCA AAGTGATTGG AAGGATGTGC AAAATAATTG ACAAGACTTG TCTGTCTCCA
    ACTCCTACAC TGGAACAGCA CCTGATGTGG GATGATATTG CCATTCTAGC ACGTTACATG
    CTGATGCTCT CCTTCAACAA TTCTCTGGAT GTGGCTGCAC ATCTCCCCTA CCTCTTTCAT
    GTGGTTACTT TGTTGGTGGC CACTGGACCC CTTTCTCTCA GAGCTTCCAC TCATGGTCTT
    GTCATCAACA TCATTCATTC TCTGTGCACT TGCTCACAGC TTCATTTTAG TGAGGAGACC
    AAGCAAGTTT TAAGACTGAG CTTGACTGAA TTTTCTCTGC CTAAATTTTA TTTGCTGTTT
    GGAATCAGCA AAGTGAAATC AGCTGCAGTC ATAGCATTCC GCTCCAGCTA TCGAGACAGG
    TCATTTTCAC CTGGCTCCTA CGAGAGGGAG ACTTTTGCAC TGACCTCCTT GGAAACTGTT
    ACTGAAGCTT TGCTGGAGAT CATGGAGGCT TGTATGAGAG ATATTCCAAC ATGCAAGTGG
    CTGGACCAGT GGACCGAACT AGCACAAAAG TTTGCATTCC AGTACAATCC ATCCCTGCAG
    CCAAGAGCAC TCGTTGTCTT TGGCTGTATA AGTAAGCGTG TCTCTCATGG ACAAATAAAA
    CAAATAATCC GAATTCTTAG CAAGGGGCTT GAGAGCTGTT TAAAAGGCCC TGATAATTAC
    AATAGCCAAG TTCTAATAGA AGCTACGGTT ATAGCACTCA CCAAACTGCA GCCTCTTCTT
    AACAAGGACT CCCCCATGCA CAAGGCCCTC TTCTGGGTAG CCATGGCAGT ACTGCAGTTG
    GATGAAGTCA ACCTATACTC AGCAGGTACA GCACTGCTTG AACAGAATCT GCATACCTTG
    GATAGCCTTC GAGTGTTCAA TGATAAGAGC CCAGAAGAGG TGTTCATGGA GATCAGGAGA
    CCACTTGAAT GGCACTGCAA ACAGATGGAT CATTTTGTTG GGCTAAATTT CAACTCCAAC
    TTCAACTTTG CACTAGTGGG GCACCTCCTG AAAGGGTACA GGCATCCTTC ACCTACCACT
    GTAGCAAGAA CAGTAAGGAT TCTACATACA CTACTAGCTC TGGTTAACAA ACACAGGAAC
    TGTGACAAGT TTGAGGTGAA CACCCAGAGC GTGGCTTACC TAGCAGCTTT GCTGACAGTG
    TCTGAGGAAG TTCGAAGTCG CTGCAGCCTA AAACATAGGA AGTCTCTCTT GCTGACAGAT
    GTTGCAATGG AAAATGTTCC TATGGATACA TATCCCATAC ATCACAGTGA CACTACCTAT
    AGGACACTAA AGGAAAATCA ACCATGGTCA TCACCAAAGG GATCAGACAG ACACCTGGCT
    GCCAGTTACC CGACAGTGGG ACAGATAAGC CCTCGAACAC GAAAGTCCAT GAGTTTGGAT
    ATGGGGCAGC CTTCACAAGC GAACACTAAA AAGCTTCTAG GTACAAGGAA AAGTTTTGAC
    CATTTGATAT CGGACACAAA GGCTCCTAAA AGGCAAGACA TGGAATCTGG AATCACAACG
    CCTCCCAAAA TGAGGAGAGT AGCCGAAAAT GATTATGAAA TGGAAACCCA GAGAATATCA
    CCGCAGCAGC ACCCCCATCT CCGGAAAGTT TCTGTTTCTG AGTCAAATGT CCTCCTGGAT
    GAAGAAGTTC TGACTGATCC AAAAATTCAA GCACTGCTGC TTACAGTTCT TGCCACGTTA
    GTGAAGTACA CTACTGATGA ATTTGACCAG CGGATTCTGT ATGAGTATTT AGCAGAAGCC
    AGTGTTGTCT TCCCAAAGGT CTTTCCTGTT GTGCATAATT TATTGGATTC CAAGATCAAT
    ACCCTCTTGT CGCTGTGTCA AGATCCAAAT TTGTTGAATC CAATTCATGG GATTGTTCAG
    AGTGTTGTCT ACCATGAGGA GTCTCCACCC CAATACCAAA CTTCTTATCT ACAAAGTTTT
    GGATTTAATG GGTTGTGGAG GTTTGCTGGC CCATTTTCCA AGCAAACACA AATCCCAGAC
    TATGCTGAGC TCATAGTGAA GTTTCTTGAT GCCTTGATTG ACACGTATTT GCCTGGAATT
    GATGAGGAAA CCAGTGAAGA ATCTCTCCTG ACACCCACGT CTCCATATCC TCCCGCAGTG
    CAGAGCCAGC TTAGCATTAC AGCTAACCTT AACCTTTCCA ATTCCATGAC CTCGCTCGCA
    ACTTCCCAGC ACTCCCCAGC TTCTCTGCCT TGCTCTAAAT CTGCAGTTTT CATGCAGCTT
    CCTCATCAAG GGATCGACAA GGAGAACGTG GAGCTCTCGC CCACCACTGG CCACACCAAC
    AGCGGGAGGA TGCGCCACGG CTCCGCCAGC CAGGTGCAAA AGCAGCGAAG TGCTGGCAGC
    TTGAAACGTC ACAGCATTAA GAAGATTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015151340.1
    CDS216..8768
    Translation

    Target ORF information:

    RefSeq Version XM_015295854.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofibromin 1 (NF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015295854.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGAGTCTGAC ATTTAAAGAG AAAATGACAA GCCTTAAATT TAAAGAAAAG
    CCTACTGACT TGGAAACCAA AAGCTACAAG TTCCTGCTGT TATCCTTGGT AAAACTGATC
    CATGCTGATC CAAAGCTTTT GCTGTGTAAT CCAAGGAAGC AAGGGCCTGA GACACAAGGC
    AGCACTGCTG AATTAATTAC AGGCCTTGTA CAACTCGTTC CACAATCCAG CATGCCAGAT
    ATAGCACAAG AAGCCATGGA GGCCTTGCTA GTTCTTCACC AGTTAGACAG CATTGACTTA
    TGGAATCCTG ATGCACCTAT AGAAACTTTC TGGGAAATTA GTTCACAAAT GCTTTTTTAT
    ATCTGCAAGA AATTAACCAG TCATCAGATG CTCAGCAGTA CAGAAATCCT CAAGTGGCTG
    CGAGAGATTC TCATCTGTAG GAATAAATTT CTTCTAAAAA ATAAACAATC AGACAGAACT
    TCCTGTCACT TTCTCTTCCT TTATGATGTC TCTGGAGGTG GAGCTAGTCA GATGTCCCTT
    GACCACGAAG AGATGATGCG CTGTGCTCCC GGAGCCACCC TCAGAAAAGG AAAAGGGAAT
    TCTTCCATGG AAAGTACAGC TGGATGCAGT GGAACACCCA TTTGTCGCCA GGCCCAAACA
    AAATTGGAAG TTGCCTTATA CATGTTTTTG TGGAGTCCGG ATATTGAGGC AGTCCTTGTT
    GCAATGTCCT GTTTCCGTCA CCTCTGTGAG GAAGCAGATA TCAGATGTGG AGTAGATGAA
    GTTTCAGTTC ATAATTTTTT GCCAAACTAC AACACTTTCA TGGAGTTTGC ATCTGTTAGC
    AATATGATGT CAACAGGGAG AGCAGCTCTT CAGAAAAGAG TGATGGCTTT ATTAAGACGT
    ATTGAACATC CAACTGCAGG GAACACAGAG GCTTGGGAGG ATACACATGC AAAATGGGAA
    CAAGCTACAA AACTAATCCT TAACTATCCA AAGACCAAAA TGGAAGACGG GCAGGTTACT
    GAAAGTCTTC ATAAGACAAT TGTTAAGAGA CGAATGTCAC ATGTTAGTGG GGGAGGCTCC
    ATAGACTTGT CTGACACAGA TTCTCTTCAG GAATGGATCA ACATGACGGG ATTCCTCTGT
    GCCCTCGGAG GAGTGTGCCT GCAGCATCGT AGCAATGCAG GATTAGCAAC CTACAGTCCA
    CCCATGGGCC CAGTCAATGA GCGTAAGGGC TCCATGATAT CTATGGTATC CACTGAGGGA
    AATGCAGAGA CTCCTGTTAG CAAATTCATA GATCGATTAC TGACTCTGAT GGTCTGTAAC
    CATGAGAAAG TGGGACTACA AATACGGACT AATATTAAAG ATCTGGTGGG TTTGGAGTTG
    AGTCCAGCAC TTTATCCAAT GCTATTTAAC AAATTGAAGA ATACCATCAG CAAGTTTTTT
    GATTCTCAAG GACAGGTTTT GTTAACTGAG ACCAACACAC AGTTTGTAGA ACAAACCATT
    GCTATAATGA AGAACTTGCT TGACAATCAT ACAGAAGGGA GCTCAGAACA TCTTGGACAA
    GCTAGTATTG AGACAATGAT GCTAAACCTG GTTAGGTATG TGCGTGTACT TGGAAATTTG
    GTACATGCAA TTCAAATAAA AACTAAACTC TGTCAGTTGG TGGAAGTAAT GATGGAGCGG
    AGAGATGACC TTTCATTTTG CCAAGAAATG AAATTTAGGA ACAAAATGGT GGAATATCTG
    ACAGACTGGG TTATGGGAAC ATCTAATCAA GCAACAGATG AAGATGTGAA GTGCTTGACA
    AGAGATTTGG ACCAAGCAAG TATGGAAGCA GTGGTCTCTC TTCTTGCTGG GCTTCCGTTA
    CAGCCTGAAG AAGGAGATGG TGTTGAGTTG ATGGAAGCCA AATCTCAGTT ATTTCTTAAA
    TATTTCACAC TGTTTATGAA CCTTCTAAAT GACTGTAGTG AAGTTGAAGA TGAAAGTGCA
    CAAACAGGTG GCAGGAAACG TGGGATGTCC CGAAGACTGG CTTCGCTGCG GCACTGCACT
    GTTCTTGCTA TGTCAAATTT ACTAAATGCA AATGTGGACA GTGGTCTTAT GCATTCAATA
    GGCTTGGGCT ATCATAAAGA CCTCCAGACA AGAGCTACAT TTATGGAAGT CCTCACCAAA
    ATTCTACAGC AGGGAACAGA ATTTGATACG TTAGCAGAAA CGGTGCTGGC AGATCGGTTT
    GAGAGGCTGG TGGAGCTAGT TACAATGATG GGTGATCAGG GGGAGCTTCC TATTGCTATG
    GCTTTAGCAA ATGTGGTGCC TTGTTCTCAG TGGGATGAGC TAGCTCGTGT TCTGGTCACA
    CTCTTTGATT CCCGGCACTT GCTCTACCAG CTACTTTGGA ATATGTTTTC AAAGGAAGTT
    GAACTGGCAG ATTCCATGCA GACACTCTTC CGAGGAAACA GCTTAGCTAG TAAAATAATG
    ACTTTCTGTT TTAAGGTATA TGGTGCTACT TATTTGCAGA AGCTTTTGGA ACCTTTATTA
    AGAACAGTGA TCACATCCTC TGAATGGCAG CATGTTAGCT TTGAGGTGGA TCCTACAAGA
    CTGGAGCCTA CAGAAAGCCT TGAAGAGAAC CAGCGGAGTC TCTTGCAAAT GACAGAAAAA
    TTTTTTCATG CAATCATTAA TTCTTCTTCG GAATTCCCAC CACAACTTCG CAGTGTGTGC
    CATTGTTTGT ACCAGGCAAC TTGCCACTCT CTACTGAATA AAGCTACCGT AAAAGAAAAA
    AAGGAAAACA AAAAATCAGT TGTTAGTCAG CGATTTCCTC AGAACAGCAT TGGAGCAGTT
    GGGAGTGCCA TGTTCCTGCG GTTCATCAAC CCTGCGATCG TCTCCCCGTA CGAGGCAGGG
    ATTTTAGATA AAAAGCCTCC GCCCAGAATT GAGAGGGGAC TGAAACTGAT GTCAAAGATA
    CTTCAGAGTA TTGCCAACCA CGTCCTGTTT ACAAAAGAAG AACATATGAG GCCTTTTAAT
    GACTTCGTAA AAAGCAATTT TGATGCAGCT CGAAGGTTTT TCCTTGATAT TGCATCTGAT
    TGCCCTGCGA GTGACACTGT CAATCACAGC CTGTCTTTCA TCAGTGATGG CAATGTTCTT
    GCTTTGCACC GGTTGTTGTG GAACAATCAG GAGAAAATTG GCCAGTACCT TTCCAGTAAT
    AGGGACCACA AAGCTGTTGG AAGGCGACCT TTTGACAAAA TGGCTACTCT TCTTGCCTAT
    CTGGGTCCAC CAGAGCACAA ACCTGTGGCA GATACACATT GGTCCAGTTT AAACCTCACC
    AGTTCGAAAT TTGAAGAATT TATGACAAGG CATCAGGTAC ATGAAAAAGA AGAGTTCAAA
    GCATTGAAAA CGTTAAATAT TTTCTACCAG GCTGGGACAT CCAAAGCTGG CAATCCTGTT
    TTCTACTACA TTGCTCGGCG TTTCAAGACT GGTCAGATCA ATGGAGATTT ATTGATATAC
    CACGTCCTGC TAACTCTGAA GCCATACTAC GCAAAGCCAT ATGAAATTGT AGTGGACCTC
    ACACATGCTG GCCCCAGTAA TCGCTTTAAA ACAGACTTTC TTTCTAAATG GTTTGTAGTT
    TTTCCAGGCT TTGCTTATGA AAATGTCTCA GCAGTTTTTA TTTATAACTG TAATTCTTGG
    GTCAGAGAAT ACACCAAATA CCACGAAAGA CTCTTGACAG GTTTGAAAGG AAGCAAAAGG
    CTTATTTTCA TAGACTCTCC AGGAAAACTG GCGGAGCACA TTGAACATGA CCAGCAGAAA
    CTTCCAGCAG CTACCTTAGC TTTGGAAGAG GACTTGAAAG TATTTCACAA TGCCCTCAAA
    TTGGCTCACA AAGACACCAA AGTGTCTATT AAAGTTGGAT CAACAGCTGT GCAGGTGACA
    TCAGCAGAAC GAACAAGAGT CCTGGGACAG ACAGTGTTTT TAAATGATAT ATACTACGCC
    TCTGAAATTG AGGAAATATG TCTTGTTGAT GAGAATCAGT TTACATTGAC GATTGCCAAC
    CAGGGAACGC CACTCACCTT CATGCATCAG GAGTGTGAAG CCATTGTGAG GTCCATTATT
    CATATACGGA CACGGTGGGA GCTGTCGCAA CCAGACTCCA TCCCACAGCA CACTAAAATT
    CGCCCTAAGG ATGTTCCCGG AACACTACTG AACATAGCAT TGCTCAACCT GGGAAGCTCA
    GACCCGAGTT TGCGGTCTGC TGCCTATAAT CTTCTGTGTG CCTTAACCTG TACCTTTAAT
    TTAAAGATTG AGGGCCAGTT ACTGGAAACT TCAGGTCTGT GTATTCCTGC CAACAACACG
    CTCTTTATTG TGTCTATTAG TAAGACTCTT GCAGCTAATG AGCCACACCT TACCCTAGAG
    TTCTTGGAAG AGTGTATTTC AGGATTTAGC AAATCTAGTA TTGAACTGAA ACATCTTTGT
    CTGGAATATA TGACTCCCTG GCTGTCCAAT CTGGTCCGAT TCTGCAAACA CAACGACGAT
    GCGAAGCGCC AGCGAGTCAC TGCCATTCTT GATAAGCTTA TAACAATGAC AATAAATGAA
    AAACAGATGT ACCCTTCTAT TCAGGCTAAA ATATGGGGAA GTCTTGGACA GATTACAGAT
    CTCCTTGACG TAGTGCTGGA CAGTTTCATC AAAACTAGTG CCACGGGGGG CTTAGGATCC
    ATTAAAGCTG AGGTTATGGC AGACACAGCA GTAGCGCTTG CTTCTGGAAA TGTAAAATTG
    GTTTCAAGCA AAGTGATTGG AAGGATGTGC AAAATAATTG ACAAGACTTG TCTGTCTCCA
    ACTCCTACAC TGGAACAGCA CCTGATGTGG GATGATATTG CCATTCTAGC ACGTTACATG
    CTGATGCTCT CCTTCAACAA TTCTCTGGAT GTGGCTGCAC ATCTCCCCTA CCTCTTTCAT
    GTGGTTACTT TGTTGGTGGC CACTGGACCC CTTTCTCTCA GAGCTTCCAC TCATGGTCTT
    GTCATCAACA TCATTCATTC TCTGTGCACT TGCTCACAGC TTCATTTTAG TGAGGAGACC
    AAGCAAGTTT TAAGACTGAG CTTGACTGAA TTTTCTCTGC CTAAATTTTA TTTGCTGTTT
    GGAATCAGCA AAGTGAAATC AGCTGCAGTC ATAGCATTCC GCTCCAGCTA TCGAGACAGG
    TCATTTTCAC CTGGCTCCTA CGAGAGGGAG ACTTTTGCAC TGACCTCCTT GGAAACTGTT
    ACTGAAGCTT TGCTGGAGAT CATGGAGGCT TGTATGAGAG ATATTCCAAC ATGCAAGTGG
    CTGGACCAGT GGACCGAACT AGCACAAAAG TTTGCATTCC AGTACAATCC ATCCCTGCAG
    CCAAGAGCAC TCGTTGTCTT TGGCTGTATA AGTAAGCGTG TCTCTCATGG ACAAATAAAA
    CAAATAATCC GAATTCTTAG CAAGGGGCTT GAGAGCTGTT TAAAAGGCCC TGATAATTAC
    AATAGCCAAG TTCTAATAGA AGCTACGGTT ATAGCACTCA CCAAACTGCA GCCTCTTCTT
    AACAAGGACT CCCCCATGCA CAAGGCCCTC TTCTGGGTAG CCATGGCAGT ACTGCAGTTG
    GATGAAGTCA ACCTATACTC AGCAGGTACA GCACTGCTTG AACAGAATCT GCATACCTTG
    GATAGCCTTC GAGTGTTCAA TGATAAGAGC CCAGAAGAGG TGTTCATGGA GATCAGGAGA
    CCACTTGAAT GGCACTGCAA ACAGATGGAT CATTTTGTTG GGCTAAATTT CAACTCCAAC
    TTCAACTTTG CACTAGTGGG GCACCTCCTG AAAGGGTACA GGCATCCTTC ACCTACCACT
    GTAGCAAGAA CAGTAAGGAT TCTACATACA CTACTAGCTC TGGTTAACAA ACACAGGAAC
    TGTGACAAGT TTGAGGTGAA CACCCAGAGC GTGGCTTACC TAGCAGCTTT GCTGACAGTG
    TCTGAGGAAG TTCGAAGTCG CTGCAGCCTA AAACATAGGA AGTCTCTCTT GCTGACAGAT
    GTTGCAATGG AAAATGTTCC TATGGATACA TATCCCATAC ATCACAGTGA CACTACCTAT
    AGGACACTAA AGGAAAATCA ACCATGGTCA TCACCAAAGG GATCAGACAG ACACCTGGCT
    GCCAGTTACC CGACAGTGGG ACAGATAAGC CCTCGAACAC GAAAGTCCAT GAGTTTGGAT
    ATGGGGCAGC CTTCACAAGC GAACACTAAA AAGCTTCTAG GTACAAGGAA AAGTTTTGAC
    CATTTGATAT CGGACACAAA GGCTCCTAAA AGGCAAGACA TGGAATCTGG AATCACAACG
    CCTCCCAAAA TGAGGAGAGT AGCCGAAAAT GATTATGAAA TGGAAACCCA GAGAATATCA
    CCGCAGCAGC ACCCCCATCT CCGGAAAGTT TCTGTTTCTG AGTCAAATGT CCTCCTGGAT
    GAAGAAGTTC TGACTGATCC AAAAATTCAA GCACTGCTGC TTACAGTTCT TGCCACGTTA
    GTGAAGTACA CTACTGATGA ATTTGACCAG CGGATTCTGT ATGAGTATTT AGCAGAAGCC
    AGTGTTGTCT TCCCAAAGGT CTTTCCTGTT GTGCATAATT TATTGGATTC CAAGATCAAT
    ACCCTCTTGT CGCTGTGTCA AGATCCAAAT TTGTTGAATC CAATTCATGG GATTGTTCAG
    AGTGTTGTCT ACCATGAGGA GTCTCCACCC CAATACCAAA CTTCTTATCT ACAAAGTTTT
    GGATTTAATG GGTTGTGGAG GTTTGCTGGC CCATTTTCCA AGCAAACACA AATCCCAGAC
    TATGCTGAGC TCATAGTGAA GTTTCTTGAT GCCTTGATTG ACACGTATTT GCCTGGAATT
    GATGAGGAAA CCAGTGAAGA ATCTCTCCTG ACACCCACGT CTCCATATCC TCCCGCAGTG
    CAGAGCCAGC TTAGCATTAC AGCTAACCTT AACCTTTCCA ATTCCATGAC CTCGCTCGCA
    ACTTCCCAGC ACTCCCCAGC TTCTCTGCCT TGCTCTAAAT CTGCAGTTTT CATGCAGCTT
    CCTCATCAAG GGATCGACAA GGAGAACGTG GAGCTCTCGC CCACCACTGG CCACACCAAC
    AGCGGGAGGA TGCGCCACGG CTCCGCCAGC CAGGTGCAAA AGCAGCGAAG TGCTGGCAGC
    TTGAAACGTC ACAGCATTAA GAAGATTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa44371
    Clone ID Related Accession (Same CDS sequence) XM_015295855.1
    Accession Version XM_015295855.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofibromin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455988.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGATGCCAAC CAGTCACTGT TTTGCATGGC AGTGGGCCTC TGTTGCTAGT
    CTGACATTTA AAGAGAAAAT GACAAGCCTT AAATTTAAAG AAAAGCCTAC TGACTTGGAA
    ACCAAAAGCT ACAAGTTCCT GCTGTTATCC TTGGTAAAAC TGATCCATGC TGATCCAAAG
    CTTTTGCTGT GTAATCCAAG GAAGCAAGGG CCTGAGACAC AAGGCAGCAC TGCTGAATTA
    ATTACAGGCC TTGTACAACT CGTTCCACAA TCCAGCATGC CAGATATAGC ACAAGAAGCC
    ATGGAGGCCT TGCTAGTTCT TCACCAGTTA GACAGCATTG ACTTATGGAA TCCTGATGCA
    CCTATAGAAA CTTTCTGGGA AATTAGTTCA CAAATGCTTT TTTATATCTG CAAGAAATTA
    ACCAGTCATC AGATGCTCAG CAGTACAGAA ATCCTCAAGT GGCTGCGAGA GATTCTCATC
    TGTAGGAATA AATTTCTTCT AAAAAATAAA CAATCAGACA GAACTTCCTG TCACTTTCTC
    TTCCTTTATG ATGTCTCTGG AGGTGGAGCT AGTCAGATGT CCCTTGACCA CGAAGAGATG
    ATGCGCTGTG CTCCCGGAGC CACCCTCAGA AAAGGAAAAG GGAATTCTTC CATGGAAAGT
    ACAGCTGGAT GCAGTGGAAC ACCCATTTGT CGCCAGGCCC AAACAAAATT GGAAGTTGCC
    TTATACATGT TTTTGTGGAG TCCGGATATT GAGGCAGTCC TTGTTGCAAT GTCCTGTTTC
    CGTCACCTCT GTGAGGAAGC AGATATCAGA TGTGGAGTAG ATGAAGTTTC AGTTCATAAT
    TTTTTGCCAA ACTACAACAC TTTCATGGAG TTTGCATCTG TTAGCAATAT GATGTCAACA
    GGGAGAGCAG CTCTTCAGAA AAGAGTGATG GCTTTATTAA GACGTATTGA ACATCCAACT
    GCAGGGAACA CAGAGGCTTG GGAGGATACA CATGCAAAAT GGGAACAAGC TACAAAACTA
    ATCCTTAACT ATCCAAAGAC CAAAATGGAA GACGGGCAGG TTACTGAAAG TCTTCATAAG
    ACAATTGTTA AGAGACGAAT GTCACATGTT AGTGGGGGAG GCTCCATAGA CTTGTCTGAC
    ACAGATTCTC TTCAGGAATG GATCAACATG ACGGGATTCC TCTGTGCCCT CGGAGGAGTG
    TGCCTGCAGC ATCGTAGCAA TGCAGGATTA GCAACCTACA GTCCACCCAT GGGCCCAGTC
    AATGAGCGTA AGGGCTCCAT GATATCTATG GTATCCACTG AGGGAAATGC AGAGACTCCT
    GTTAGCAAAT TCATAGATCG ATTACTGACT CTGATGGTCT GTAACCATGA GAAAGTGGGA
    CTACAAATAC GGACTAATAT TAAAGATCTG GTGGGTTTGG AGTTGAGTCC AGCACTTTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGATTC TCAAGGACAG
    GTTTTGTTAA CTGAGACCAA CACACAGTTT GTAGAACAAA CCATTGCTAT AATGAAGAAC
    TTGCTTGACA ATCATACAGA AGGGAGCTCA GAACATCTTG GACAAGCTAG TATTGAGACA
    ATGATGCTAA ACCTGGTTAG GTATGTGCGT GTACTTGGAA ATTTGGTACA TGCAATTCAA
    ATAAAAACTA AACTCTGTCA GTTGGTGGAA GTAATGATGG AGCGGAGAGA TGACCTTTCA
    TTTTGCCAAG AAATGAAATT TAGGAACAAA ATGGTGGAAT ATCTGACAGA CTGGGTTATG
    GGAACATCTA ATCAAGCAAC AGATGAAGAT GTGAAGTGCT TGACAAGAGA TTTGGACCAA
    GCAAGTATGG AAGCAGTGGT CTCTCTTCTT GCTGGGCTTC CGTTACAGCC TGAAGAAGGA
    GATGGTGTTG AGTTGATGGA AGCCAAATCT CAGTTATTTC TTAAATATTT CACACTGTTT
    ATGAACCTTC TAAATGACTG TAGTGAAGTT GAAGATGAAA GTGCACAAAC AGGTGGCAGG
    AAACGTGGGA TGTCCCGAAG ACTGGCTTCG CTGCGGCACT GCACTGTTCT TGCTATGTCA
    AATTTACTAA ATGCAAATGT GGACAGTGGT CTTATGCATT CAATAGGCTT GGGCTATCAT
    AAAGACCTCC AGACAAGAGC TACATTTATG GAAGTCCTCA CCAAAATTCT ACAGCAGGGA
    ACAGAATTTG ATACGTTAGC AGAAACGGTG CTGGCAGATC GGTTTGAGAG GCTGGTGGAG
    CTAGTTACAA TGATGGGTGA TCAGGGGGAG CTTCCTATTG CTATGGCTTT AGCAAATGTG
    GTGCCTTGTT CTCAGTGGGA TGAGCTAGCT CGTGTTCTGG TCACACTCTT TGATTCCCGG
    CACTTGCTCT ACCAGCTACT TTGGAATATG TTTTCAAAGG AAGTTGAACT GGCAGATTCC
    ATGCAGACAC TCTTCCGAGG AAACAGCTTA GCTAGTAAAA TAATGACTTT CTGTTTTAAG
    GTATATGGTG CTACTTATTT GCAGAAGCTT TTGGAACCTT TATTAAGAAC AGTGATCACA
    TCCTCTGAAT GGCAGCATGT TAGCTTTGAG GTGGATCCTA CAAGACTGGA GCCTACAGAA
    AGCCTTGAAG AGAACCAGCG GAGTCTCTTG CAAATGACAG AAAAATTTTT TCATGCAATC
    ATTAATTCTT CTTCGGAATT CCCACCACAA CTTCGCAGTG TGTGCCATTG TTTGTACCAG
    GTTGTTAGTC AGCGATTTCC TCAGAACAGC ATTGGAGCAG TTGGGAGTGC CATGTTCCTG
    CGGTTCATCA ACCCTGCGAT CGTCTCCCCG TACGAGGCAG GGATTTTAGA TAAAAAGCCT
    CCGCCCAGAA TTGAGAGGGG ACTGAAACTG ATGTCAAAGA TACTTCAGAG TATTGCCAAC
    CACGTCCTGT TTACAAAAGA AGAACATATG AGGCCTTTTA ATGACTTCGT AAAAAGCAAT
    TTTGATGCAG CTCGAAGGTT TTTCCTTGAT ATTGCATCTG ATTGCCCTGC GAGTGACACT
    GTCAATCACA GCCTGTCTTT CATCAGTGAT GGCAATGTTC TTGCTTTGCA CCGGTTGTTG
    TGGAACAATC AGGAGAAAAT TGGCCAGTAC CTTTCCAGTA ATAGGGACCA CAAAGCTGTT
    GGAAGGCGAC CTTTTGACAA AATGGCTACT CTTCTTGCCT ATCTGGGTCC ACCAGAGCAC
    AAACCTGTGG CAGATACACA TTGGTCCAGT TTAAACCTCA CCAGTTCGAA ATTTGAAGAA
    TTTATGACAA GGCATCAGGT ACATGAAAAA GAAGAGTTCA AAGCATTGAA AACGTTAAAT
    ATTTTCTACC AGGCTGGGAC ATCCAAAGCT GGCAATCCTG TTTTCTACTA CATTGCTCGG
    CGTTTCAAGA CTGGTCAGAT CAATGGAGAT TTATTGATAT ACCACGTCCT GCTAACTCTG
    AAGCCATACT ACGCAAAGCC ATATGAAATT GTAGTGGACC TCACACATGC TGGCCCCAGT
    AATCGCTTTA AAACAGACTT TCTTTCTAAA TGGTTTGTAG TTTTTCCAGG CTTTGCTTAT
    GAAAATGTCT CAGCAGTTTT TATTTATAAC TGTAATTCTT GGGTCAGAGA ATACACCAAA
    TACCACGAAA GACTCTTGAC AGGTTTGAAA GGAAGCAAAA GGCTTATTTT CATAGACTCT
    CCAGGAAAAC TGGCGGAGCA CATTGAACAT GACCAGCAGA AACTTCCAGC AGCTACCTTA
    GCTTTGGAAG AGGACTTGAA AGTATTTCAC AATGCCCTCA AATTGGCTCA CAAAGACACC
    AAAGTGTCTA TTAAAGTTGG ATCAACAGCT GTGCAGGTGA CATCAGCAGA ACGAACAAGA
    GTCCTGGGAC AGACAGTGTT TTTAAATGAT ATATACTACG CCTCTGAAAT TGAGGAAATA
    TGTCTTGTTG ATGAGAATCA GTTTACATTG ACGATTGCCA ACCAGGGAAC GCCACTCACC
    TTCATGCATC AGGAGTGTGA AGCCATTGTG AGGTCCATTA TTCATATACG GACACGGTGG
    GAGCTGTCGC AACCAGACTC CATCCCACAG CACACTAAAA TTCGCCCTAA GGATGTTCCC
    GGAACACTAC TGAACATAGC ATTGCTCAAC CTGGGAAGCT CAGACCCGAG TTTGCGGTCT
    GCTGCCTATA ATCTTCTGTG TGCCTTAACC TGTACCTTTA ATTTAAAGAT TGAGGGCCAG
    TTACTGGAAA CTTCAGGTCT GTGTATTCCT GCCAACAACA CGCTCTTTAT TGTGTCTATT
    AGTAAGACTC TTGCAGCTAA TGAGCCACAC CTTACCCTAG AGTTCTTGGA AGAGTGTATT
    TCAGGATTTA GCAAATCTAG TATTGAACTG AAACATCTTT GTCTGGAATA TATGACTCCC
    TGGCTGTCCA ATCTGGTCCG ATTCTGCAAA CACAACGACG ATGCGAAGCG CCAGCGAGTC
    ACTGCCATTC TTGATAAGCT TATAACAATG ACAATAAATG AAAAACAGAT GTACCCTTCT
    ATTCAGGCTA AAATATGGGG AAGTCTTGGA CAGATTACAG ATCTCCTTGA CGTAGTGCTG
    GACAGTTTCA TCAAAACTAG TGCCACGGGG GGCTTAGGAT CCATTAAAGC TGAGGTTATG
    GCAGACACAG CAGTAGCGCT TGCTTCTGGA AATGTAAAAT TGGTTTCAAG CAAAGTGATT
    GGAAGGATGT GCAAAATAAT TGACAAGACT TGTCTGTCTC CAACTCCTAC ACTGGAACAG
    CACCTGATGT GGGATGATAT TGCCATTCTA GCACGTTACA TGCTGATGCT CTCCTTCAAC
    AATTCTCTGG ATGTGGCTGC ACATCTCCCC TACCTCTTTC ATGTGGTTAC TTTGTTGGTG
    GCCACTGGAC CCCTTTCTCT CAGAGCTTCC ACTCATGGTC TTGTCATCAA CATCATTCAT
    TCTCTGTGCA CTTGCTCACA GCTTCATTTT AGTGAGGAGA CCAAGCAAGT TTTAAGACTG
    AGCTTGACTG AATTTTCTCT GCCTAAATTT TATTTGCTGT TTGGAATCAG CAAAGTGAAA
    TCAGCTGCAG TCATAGCATT CCGCTCCAGC TATCGAGACA GGTCATTTTC ACCTGGCTCC
    TACGAGAGGG AGACTTTTGC ACTGACCTCC TTGGAAACTG TTACTGAAGC TTTGCTGGAG
    ATCATGGAGG CTTGTATGAG AGATATTCCA ACATGCAAGT GGCTGGACCA GTGGACCGAA
    CTAGCACAAA AGTTTGCATT CCAGTACAAT CCATCCCTGC AGCCAAGAGC ACTCGTTGTC
    TTTGGCTGTA TAAGTAAGCG TGTCTCTCAT GGACAAATAA AACAAATAAT CCGAATTCTT
    AGCAAGGGGC TTGAGAGCTG TTTAAAAGGC CCTGATAATT ACAATAGCCA AGTTCTAATA
    GAAGCTACGG TTATAGCACT CACCAAACTG CAGCCTCTTC TTAACAAGGA CTCCCCCATG
    CACAAGGCCC TCTTCTGGGT AGCCATGGCA GTACTGCAGT TGGATGAAGT CAACCTATAC
    TCAGCAGGTA CAGCACTGCT TGAACAGAAT CTGCATACCT TGGATAGCCT TCGAGTGTTC
    AATGATAAGA GCCCAGAAGA GGTGTTCATG GAGATCAGGA GACCACTTGA ATGGCACTGC
    AAACAGATGG ATCATTTTGT TGGGCTAAAT TTCAACTCCA ACTTCAACTT TGCACTAGTG
    GGGCACCTCC TGAAAGGGTA CAGGCATCCT TCACCTACCA CTGTAGCAAG AACAGTAAGG
    ATTCTACATA CACTACTAGC TCTGGTTAAC AAACACAGGA ACTGTGACAA GTTTGAGGTG
    AACACCCAGA GCGTGGCTTA CCTAGCAGCT TTGCTGACAG TGTCTGAGGA AGTTCGAAGT
    CGCTGCAGCC TAAAACATAG GAAGTCTCTC TTGCTGACAG ATGTTGCAAT GGAAAATGTT
    CCTATGGATA CATATCCCAT ACATCACAGT GACACTACCT ATAGGACACT AAAGGAAAAT
    CAACCATGGT CATCACCAAA GGGATCAGAC AGACACCTGG CTGCCAGTTA CCCGACAGTG
    GGACAGATAA GCCCTCGAAC ACGAAAGTCC ATGAGTTTGG ATATGGGGCA GCCTTCACAA
    GCGAACACTA AAAAGCTTCT AGGTACAAGG AAAAGTTTTG ACCATTTGAT ATCGGACACA
    AAGGCTCCTA AAAGGCAAGA CATGGAATCT GGAATCACAA CGCCTCCCAA AATGAGGAGA
    GTAGCCGAAA ATGATTATGA AATGGAAACC CAGAGAATAT CACCGCAGCA GCACCCCCAT
    CTCCGGAAAG TTTCTGTTTC TGAGTCAAAT GTCCTCCTGG ATGAAGAAGT TCTGACTGAT
    CCAAAAATTC AAGCACTGCT GCTTACAGTT CTTGCCACGT TAGTGAAGTA CACTACTGAT
    GAATTTGACC AGCGGATTCT GTATGAGTAT TTAGCAGAAG CCAGTGTTGT CTTCCCAAAG
    GTCTTTCCTG TTGTGCATAA TTTATTGGAT TCCAAGATCA ATACCCTCTT GTCGCTGTGT
    CAAGATCCAA ATTTGTTGAA TCCAATTCAT GGGATTGTTC AGAGTGTTGT CTACCATGAG
    GAGTCTCCAC CCCAATACCA AACTTCTTAT CTACAAAGTT TTGGATTTAA TGGGTTGTGG
    AGGTTTGCTG GCCCATTTTC CAAGCAAACA CAAATCCCAG ACTATGCTGA GCTCATAGTG
    AAGTTTCTTG ATGCCTTGAT TGACACGTAT TTGCCTGGAA TTGATGAGGA AACCAGTGAA
    GAATCTCTCC TGACACCCAC GTCTCCATAT CCTCCCGCAG TGCAGAGCCA GCTTAGCATT
    ACAGCTAACC TTAACCTTTC CAATTCCATG ACCTCGCTCG CAACTTCCCA GCACTCCCCA
    GCTTCTCTGC CTTGCTCTAA ATCTGCAGTT TTCATGCAGC TTCCTCATCA AGGGATCGAC
    AAGGAGAACG TGGAGCTCTC GCCCACCACT GGCCACACCA ACAGCGGGAG GATGCGCCAC
    GGCTCCGCCA GCCAGGTGCA AAAGCAGCGA AGTGCTGGCA GCTTGAAACG TCACAGCATT
    AAGAAGATTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015151341.1
    CDS217..8751
    Translation

    Target ORF information:

    RefSeq Version XM_015295855.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofibromin 1 (NF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015295855.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCGGCCC ACAAGCCGGT GGAGTGGGTG CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AGGCCAGCCA GCAGAATACC CACACCAAAG TCAGCACGGA GCACAACAAG
    GAGTGTCTCA TCAACATTTC CAAGTACAAG TTCTCTCTGG TCATCAGTGG TCTCACTAAT
    ATCTTAAAAA ATGTTAATAA CATGAGAATA TTTGGAGAAA CTGCTGAAAA GAACTTATAT
    CTATCTCAAC TGATTATCTT GGATACACTG GAAAAATGTC TAGCAGGGCA ACCGAAGGAT
    ACGATGCGAC TAGACGAGAC TATGCTGGTA AAACAGTTGC TGCCAGAAAT CTGCCACTTC
    ATCCACACTT ACCGTGAAGG GAACCAGCAT GCAGCTGAAC TACGCAATTC TGCCTCTGGG
    GTTCTTTTTT CTCTTAGCTG CAATAACTTT AACGCTGTGT TCAGCCGCAT TTCCACCAGA
    TTGCAGGAAC TGACTGTTTG CTCAGAAGAT AATGCCGATG TTCACGACAT TGAACTTCTA
    CAGTACATCA GTGTGGATTG TTCAAAGCTA AAAAGACTCT TGCAAGAGAC TGTATTCAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGTTA GCTGTTATCA ACAGTCTTGA AAAGGCATTT
    TGGAACTGGG TGGAAAACTA TCCTGATGAA TTCACAAAGC TGTATCAAAC ACCACAGACT
    GATATGGCCG ATTGTGCAGA GAAGTTGTTT GACTTAGTGG ATGGCTTTGC AGAGAGCACA
    AAACGTAAAG CAGCGGTGTG GCCACTACAA ATCATACTCC TGGTCCTGTG TCCAGAGATA
    ATTCAAGATA TAGCAAAGGA TGTAGTAGAG GAAACTAAAA TGAACAAGAA ACTGTTCCTG
    GACAATCTCA GGAAAGCACT TGCAGGCCAC AGTGGGAGCA GGCAGCTGAC TGAAAGCGCT
    GCTATTGCTT GTGTTAAACT GTGCAAAGCA TCTACCTACA TCAACTGGGA GGATAATTCT
    GTCATTTTTC TACTAGTGCA GTCCATGGTA GTAGATCTTA AGAATTTGCT GTTTAATCCG
    AGCAAACCTT TTTCAAGAAG CAATCAGAAT GCAGATGTAG ACCTTATGAT CGACTGCTTA
    GTTTCATGCT TCCGCATAAA CCCGCATAAT AACCAACACT TTAAGATCTG CTTGGCACAG
    AGTTCCCCAT CAACATTTCA TTATGTACTA GTAAATTCAC TCCACCGAAT AATCACAAAT
    TCTGCATTGG ACTGGTGGCC CAGGATTGAT GCTGTGTACT GCCATTCCGT GGAGCTTCGC
    AGCATGTTTA GTGAGACACT TCACAAAGCT ATTCAGGGCT GTGGGGCACA TCCAGCAATT
    CGCATGACTC CGATGCCAAC CAGTCACTGT TTTGCATGGC AGTGGGCCTC TGTTGCTAGT
    CTGACATTTA AAGAGAAAAT GACAAGCCTT AAATTTAAAG AAAAGCCTAC TGACTTGGAA
    ACCAAAAGCT ACAAGTTCCT GCTGTTATCC TTGGTAAAAC TGATCCATGC TGATCCAAAG
    CTTTTGCTGT GTAATCCAAG GAAGCAAGGG CCTGAGACAC AAGGCAGCAC TGCTGAATTA
    ATTACAGGCC TTGTACAACT CGTTCCACAA TCCAGCATGC CAGATATAGC ACAAGAAGCC
    ATGGAGGCCT TGCTAGTTCT TCACCAGTTA GACAGCATTG ACTTATGGAA TCCTGATGCA
    CCTATAGAAA CTTTCTGGGA AATTAGTTCA CAAATGCTTT TTTATATCTG CAAGAAATTA
    ACCAGTCATC AGATGCTCAG CAGTACAGAA ATCCTCAAGT GGCTGCGAGA GATTCTCATC
    TGTAGGAATA AATTTCTTCT AAAAAATAAA CAATCAGACA GAACTTCCTG TCACTTTCTC
    TTCCTTTATG ATGTCTCTGG AGGTGGAGCT AGTCAGATGT CCCTTGACCA CGAAGAGATG
    ATGCGCTGTG CTCCCGGAGC CACCCTCAGA AAAGGAAAAG GGAATTCTTC CATGGAAAGT
    ACAGCTGGAT GCAGTGGAAC ACCCATTTGT CGCCAGGCCC AAACAAAATT GGAAGTTGCC
    TTATACATGT TTTTGTGGAG TCCGGATATT GAGGCAGTCC TTGTTGCAAT GTCCTGTTTC
    CGTCACCTCT GTGAGGAAGC AGATATCAGA TGTGGAGTAG ATGAAGTTTC AGTTCATAAT
    TTTTTGCCAA ACTACAACAC TTTCATGGAG TTTGCATCTG TTAGCAATAT GATGTCAACA
    GGGAGAGCAG CTCTTCAGAA AAGAGTGATG GCTTTATTAA GACGTATTGA ACATCCAACT
    GCAGGGAACA CAGAGGCTTG GGAGGATACA CATGCAAAAT GGGAACAAGC TACAAAACTA
    ATCCTTAACT ATCCAAAGAC CAAAATGGAA GACGGGCAGG TTACTGAAAG TCTTCATAAG
    ACAATTGTTA AGAGACGAAT GTCACATGTT AGTGGGGGAG GCTCCATAGA CTTGTCTGAC
    ACAGATTCTC TTCAGGAATG GATCAACATG ACGGGATTCC TCTGTGCCCT CGGAGGAGTG
    TGCCTGCAGC ATCGTAGCAA TGCAGGATTA GCAACCTACA GTCCACCCAT GGGCCCAGTC
    AATGAGCGTA AGGGCTCCAT GATATCTATG GTATCCACTG AGGGAAATGC AGAGACTCCT
    GTTAGCAAAT TCATAGATCG ATTACTGACT CTGATGGTCT GTAACCATGA GAAAGTGGGA
    CTACAAATAC GGACTAATAT TAAAGATCTG GTGGGTTTGG AGTTGAGTCC AGCACTTTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGATTC TCAAGGACAG
    GTTTTGTTAA CTGAGACCAA CACACAGTTT GTAGAACAAA CCATTGCTAT AATGAAGAAC
    TTGCTTGACA ATCATACAGA AGGGAGCTCA GAACATCTTG GACAAGCTAG TATTGAGACA
    ATGATGCTAA ACCTGGTTAG GTATGTGCGT GTACTTGGAA ATTTGGTACA TGCAATTCAA
    ATAAAAACTA AACTCTGTCA GTTGGTGGAA GTAATGATGG AGCGGAGAGA TGACCTTTCA
    TTTTGCCAAG AAATGAAATT TAGGAACAAA ATGGTGGAAT ATCTGACAGA CTGGGTTATG
    GGAACATCTA ATCAAGCAAC AGATGAAGAT GTGAAGTGCT TGACAAGAGA TTTGGACCAA
    GCAAGTATGG AAGCAGTGGT CTCTCTTCTT GCTGGGCTTC CGTTACAGCC TGAAGAAGGA
    GATGGTGTTG AGTTGATGGA AGCCAAATCT CAGTTATTTC TTAAATATTT CACACTGTTT
    ATGAACCTTC TAAATGACTG TAGTGAAGTT GAAGATGAAA GTGCACAAAC AGGTGGCAGG
    AAACGTGGGA TGTCCCGAAG ACTGGCTTCG CTGCGGCACT GCACTGTTCT TGCTATGTCA
    AATTTACTAA ATGCAAATGT GGACAGTGGT CTTATGCATT CAATAGGCTT GGGCTATCAT
    AAAGACCTCC AGACAAGAGC TACATTTATG GAAGTCCTCA CCAAAATTCT ACAGCAGGGA
    ACAGAATTTG ATACGTTAGC AGAAACGGTG CTGGCAGATC GGTTTGAGAG GCTGGTGGAG
    CTAGTTACAA TGATGGGTGA TCAGGGGGAG CTTCCTATTG CTATGGCTTT AGCAAATGTG
    GTGCCTTGTT CTCAGTGGGA TGAGCTAGCT CGTGTTCTGG TCACACTCTT TGATTCCCGG
    CACTTGCTCT ACCAGCTACT TTGGAATATG TTTTCAAAGG AAGTTGAACT GGCAGATTCC
    ATGCAGACAC TCTTCCGAGG AAACAGCTTA GCTAGTAAAA TAATGACTTT CTGTTTTAAG
    GTATATGGTG CTACTTATTT GCAGAAGCTT TTGGAACCTT TATTAAGAAC AGTGATCACA
    TCCTCTGAAT GGCAGCATGT TAGCTTTGAG GTGGATCCTA CAAGACTGGA GCCTACAGAA
    AGCCTTGAAG AGAACCAGCG GAGTCTCTTG CAAATGACAG AAAAATTTTT TCATGCAATC
    ATTAATTCTT CTTCGGAATT CCCACCACAA CTTCGCAGTG TGTGCCATTG TTTGTACCAG
    GTTGTTAGTC AGCGATTTCC TCAGAACAGC ATTGGAGCAG TTGGGAGTGC CATGTTCCTG
    CGGTTCATCA ACCCTGCGAT CGTCTCCCCG TACGAGGCAG GGATTTTAGA TAAAAAGCCT
    CCGCCCAGAA TTGAGAGGGG ACTGAAACTG ATGTCAAAGA TACTTCAGAG TATTGCCAAC
    CACGTCCTGT TTACAAAAGA AGAACATATG AGGCCTTTTA ATGACTTCGT AAAAAGCAAT
    TTTGATGCAG CTCGAAGGTT TTTCCTTGAT ATTGCATCTG ATTGCCCTGC GAGTGACACT
    GTCAATCACA GCCTGTCTTT CATCAGTGAT GGCAATGTTC TTGCTTTGCA CCGGTTGTTG
    TGGAACAATC AGGAGAAAAT TGGCCAGTAC CTTTCCAGTA ATAGGGACCA CAAAGCTGTT
    GGAAGGCGAC CTTTTGACAA AATGGCTACT CTTCTTGCCT ATCTGGGTCC ACCAGAGCAC
    AAACCTGTGG CAGATACACA TTGGTCCAGT TTAAACCTCA CCAGTTCGAA ATTTGAAGAA
    TTTATGACAA GGCATCAGGT ACATGAAAAA GAAGAGTTCA AAGCATTGAA AACGTTAAAT
    ATTTTCTACC AGGCTGGGAC ATCCAAAGCT GGCAATCCTG TTTTCTACTA CATTGCTCGG
    CGTTTCAAGA CTGGTCAGAT CAATGGAGAT TTATTGATAT ACCACGTCCT GCTAACTCTG
    AAGCCATACT ACGCAAAGCC ATATGAAATT GTAGTGGACC TCACACATGC TGGCCCCAGT
    AATCGCTTTA AAACAGACTT TCTTTCTAAA TGGTTTGTAG TTTTTCCAGG CTTTGCTTAT
    GAAAATGTCT CAGCAGTTTT TATTTATAAC TGTAATTCTT GGGTCAGAGA ATACACCAAA
    TACCACGAAA GACTCTTGAC AGGTTTGAAA GGAAGCAAAA GGCTTATTTT CATAGACTCT
    CCAGGAAAAC TGGCGGAGCA CATTGAACAT GACCAGCAGA AACTTCCAGC AGCTACCTTA
    GCTTTGGAAG AGGACTTGAA AGTATTTCAC AATGCCCTCA AATTGGCTCA CAAAGACACC
    AAAGTGTCTA TTAAAGTTGG ATCAACAGCT GTGCAGGTGA CATCAGCAGA ACGAACAAGA
    GTCCTGGGAC AGACAGTGTT TTTAAATGAT ATATACTACG CCTCTGAAAT TGAGGAAATA
    TGTCTTGTTG ATGAGAATCA GTTTACATTG ACGATTGCCA ACCAGGGAAC GCCACTCACC
    TTCATGCATC AGGAGTGTGA AGCCATTGTG AGGTCCATTA TTCATATACG GACACGGTGG
    GAGCTGTCGC AACCAGACTC CATCCCACAG CACACTAAAA TTCGCCCTAA GGATGTTCCC
    GGAACACTAC TGAACATAGC ATTGCTCAAC CTGGGAAGCT CAGACCCGAG TTTGCGGTCT
    GCTGCCTATA ATCTTCTGTG TGCCTTAACC TGTACCTTTA ATTTAAAGAT TGAGGGCCAG
    TTACTGGAAA CTTCAGGTCT GTGTATTCCT GCCAACAACA CGCTCTTTAT TGTGTCTATT
    AGTAAGACTC TTGCAGCTAA TGAGCCACAC CTTACCCTAG AGTTCTTGGA AGAGTGTATT
    TCAGGATTTA GCAAATCTAG TATTGAACTG AAACATCTTT GTCTGGAATA TATGACTCCC
    TGGCTGTCCA ATCTGGTCCG ATTCTGCAAA CACAACGACG ATGCGAAGCG CCAGCGAGTC
    ACTGCCATTC TTGATAAGCT TATAACAATG ACAATAAATG AAAAACAGAT GTACCCTTCT
    ATTCAGGCTA AAATATGGGG AAGTCTTGGA CAGATTACAG ATCTCCTTGA CGTAGTGCTG
    GACAGTTTCA TCAAAACTAG TGCCACGGGG GGCTTAGGAT CCATTAAAGC TGAGGTTATG
    GCAGACACAG CAGTAGCGCT TGCTTCTGGA AATGTAAAAT TGGTTTCAAG CAAAGTGATT
    GGAAGGATGT GCAAAATAAT TGACAAGACT TGTCTGTCTC CAACTCCTAC ACTGGAACAG
    CACCTGATGT GGGATGATAT TGCCATTCTA GCACGTTACA TGCTGATGCT CTCCTTCAAC
    AATTCTCTGG ATGTGGCTGC ACATCTCCCC TACCTCTTTC ATGTGGTTAC TTTGTTGGTG
    GCCACTGGAC CCCTTTCTCT CAGAGCTTCC ACTCATGGTC TTGTCATCAA CATCATTCAT
    TCTCTGTGCA CTTGCTCACA GCTTCATTTT AGTGAGGAGA CCAAGCAAGT TTTAAGACTG
    AGCTTGACTG AATTTTCTCT GCCTAAATTT TATTTGCTGT TTGGAATCAG CAAAGTGAAA
    TCAGCTGCAG TCATAGCATT CCGCTCCAGC TATCGAGACA GGTCATTTTC ACCTGGCTCC
    TACGAGAGGG AGACTTTTGC ACTGACCTCC TTGGAAACTG TTACTGAAGC TTTGCTGGAG
    ATCATGGAGG CTTGTATGAG AGATATTCCA ACATGCAAGT GGCTGGACCA GTGGACCGAA
    CTAGCACAAA AGTTTGCATT CCAGTACAAT CCATCCCTGC AGCCAAGAGC ACTCGTTGTC
    TTTGGCTGTA TAAGTAAGCG TGTCTCTCAT GGACAAATAA AACAAATAAT CCGAATTCTT
    AGCAAGGGGC TTGAGAGCTG TTTAAAAGGC CCTGATAATT ACAATAGCCA AGTTCTAATA
    GAAGCTACGG TTATAGCACT CACCAAACTG CAGCCTCTTC TTAACAAGGA CTCCCCCATG
    CACAAGGCCC TCTTCTGGGT AGCCATGGCA GTACTGCAGT TGGATGAAGT CAACCTATAC
    TCAGCAGGTA CAGCACTGCT TGAACAGAAT CTGCATACCT TGGATAGCCT TCGAGTGTTC
    AATGATAAGA GCCCAGAAGA GGTGTTCATG GAGATCAGGA GACCACTTGA ATGGCACTGC
    AAACAGATGG ATCATTTTGT TGGGCTAAAT TTCAACTCCA ACTTCAACTT TGCACTAGTG
    GGGCACCTCC TGAAAGGGTA CAGGCATCCT TCACCTACCA CTGTAGCAAG AACAGTAAGG
    ATTCTACATA CACTACTAGC TCTGGTTAAC AAACACAGGA ACTGTGACAA GTTTGAGGTG
    AACACCCAGA GCGTGGCTTA CCTAGCAGCT TTGCTGACAG TGTCTGAGGA AGTTCGAAGT
    CGCTGCAGCC TAAAACATAG GAAGTCTCTC TTGCTGACAG ATGTTGCAAT GGAAAATGTT
    CCTATGGATA CATATCCCAT ACATCACAGT GACACTACCT ATAGGACACT AAAGGAAAAT
    CAACCATGGT CATCACCAAA GGGATCAGAC AGACACCTGG CTGCCAGTTA CCCGACAGTG
    GGACAGATAA GCCCTCGAAC ACGAAAGTCC ATGAGTTTGG ATATGGGGCA GCCTTCACAA
    GCGAACACTA AAAAGCTTCT AGGTACAAGG AAAAGTTTTG ACCATTTGAT ATCGGACACA
    AAGGCTCCTA AAAGGCAAGA CATGGAATCT GGAATCACAA CGCCTCCCAA AATGAGGAGA
    GTAGCCGAAA ATGATTATGA AATGGAAACC CAGAGAATAT CACCGCAGCA GCACCCCCAT
    CTCCGGAAAG TTTCTGTTTC TGAGTCAAAT GTCCTCCTGG ATGAAGAAGT TCTGACTGAT
    CCAAAAATTC AAGCACTGCT GCTTACAGTT CTTGCCACGT TAGTGAAGTA CACTACTGAT
    GAATTTGACC AGCGGATTCT GTATGAGTAT TTAGCAGAAG CCAGTGTTGT CTTCCCAAAG
    GTCTTTCCTG TTGTGCATAA TTTATTGGAT TCCAAGATCA ATACCCTCTT GTCGCTGTGT
    CAAGATCCAA ATTTGTTGAA TCCAATTCAT GGGATTGTTC AGAGTGTTGT CTACCATGAG
    GAGTCTCCAC CCCAATACCA AACTTCTTAT CTACAAAGTT TTGGATTTAA TGGGTTGTGG
    AGGTTTGCTG GCCCATTTTC CAAGCAAACA CAAATCCCAG ACTATGCTGA GCTCATAGTG
    AAGTTTCTTG ATGCCTTGAT TGACACGTAT TTGCCTGGAA TTGATGAGGA AACCAGTGAA
    GAATCTCTCC TGACACCCAC GTCTCCATAT CCTCCCGCAG TGCAGAGCCA GCTTAGCATT
    ACAGCTAACC TTAACCTTTC CAATTCCATG ACCTCGCTCG CAACTTCCCA GCACTCCCCA
    GCTTCTCTGC CTTGCTCTAA ATCTGCAGTT TTCATGCAGC TTCCTCATCA AGGGATCGAC
    AAGGAGAACG TGGAGCTCTC GCCCACCACT GGCCACACCA ACAGCGGGAG GATGCGCCAC
    GGCTCCGCCA GCCAGGTGCA AAAGCAGCGA AGTGCTGGCA GCTTGAAACG TCACAGCATT
    AAGAAGATTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa44372
    Clone ID Related Accession (Same CDS sequence) XM_015295856.1
    Accession Version XM_015295856.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofibromin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455988.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
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    2521
    2581
    2641
    2701
    2761
    2821
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    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
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    3601
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    3721
    3781
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGTGGCCTG GGGTAAGAAG GGGCACACAG CAACCGAAGG ATACGATGCG ACTAGACGAG 
    ACTATGCTGG TAAAACAGTT GCTGCCAGAA ATCTGCCACT TCATCCACAC TTACCGTGAA
    GGGAACCAGC ATGCAGCTGA ACTACGCAAT TCTGCCTCTG GGGTTCTTTT TTCTCTTAGC
    TGCAATAACT TTAACGCTGT GTTCAGCCGC ATTTCCACCA GATTGCAGGA ACTGACTGTT
    TGCTCAGAAG ATAATGCCGA TGTTCACGAC ATTGAACTTC TACAGTACAT CAGTGTGGAT
    TGTTCAAAGC TAAAAAGACT CTTGCAAGAG ACTGTATTCA AGTTTAAAGC CCTTAAGAAA
    GTAGCTCAGT TAGCTGTTAT CAACAGTCTT GAAAAGGCAT TTTGGAACTG GGTGGAAAAC
    TATCCTGATG AATTCACAAA GCTGTATCAA ACACCACAGA CTGATATGGC CGATTGTGCA
    GAGAAGTTGT TTGACTTAGT GGATGGCTTT GCAGAGAGCA CAAAACGTAA AGCAGCGGTG
    TGGCCACTAC AAATCATACT CCTGGTCCTG TGTCCAGAGA TAATTCAAGA TATAGCAAAG
    GATGTAGTAG AGGAAACTAA AATGAACAAG AAACTGTTCC TGGACAATCT CAGGAAAGCA
    CTTGCAGGCC ACAGTGGGAG CAGGCAGCTG ACTGAAAGCG CTGCTATTGC TTGTGTTAAA
    CTGTGCAAAG CATCTACCTA CATCAACTGG GAGGATAATT CTGTCATTTT TCTACTAGTG
    CAGTCCATGG TAGTAGATCT TAAGAATTTG CTGTTTAATC CGAGCAAACC TTTTTCAAGA
    AGCAATCAGA ATGCAGATGT AGACCTTATG ATCGACTGCT TAGTTTCATG CTTCCGCATA
    AACCCGCATA ATAACCAACA CTTTAAGATC TGCTTGGCAC AGAGTTCCCC ATCAACATTT
    CATTATGTAC TAGTAAATTC ACTCCACCGA ATAATCACAA ATTCTGCATT GGACTGGTGG
    CCCAGGATTG ATGCTGTGTA CTGCCATTCC GTGGAGCTTC GCAGCATGTT TAGTGAGACA
    CTTCACAAAG CTATTCAGGG CTGTGGGGCA CATCCAGCAA TTCGCATGAC TCCGATGCCA
    ACCAGTCACT GTTTTGCATG GCAGTGGGCC TCTGTTGCTA GTCTGACATT TAAAGAGAAA
    ATGACAAGCC TTAAATTTAA AGAAAAGCCT ACTGACTTGG AAACCAAAAG CTACAAGTTC
    CTGCTGTTAT CCTTGGTAAA ACTGATCCAT GCTGATCCAA AGCTTTTGCT GTGTAATCCA
    AGGAAGCAAG GGCCTGAGAC ACAAGGCAGC ACTGCTGAAT TAATTACAGG CCTTGTACAA
    CTCGTTCCAC AATCCAGCAT GCCAGATATA GCACAAGAAG CCATGGAGGC CTTGCTAGTT
    CTTCACCAGT TAGACAGCAT TGACTTATGG AATCCTGATG CACCTATAGA AACTTTCTGG
    GAAATTAGTT CACAAATGCT TTTTTATATC TGCAAGAAAT TAACCAGTCA TCAGATGCTC
    AGCAGTACAG AAATCCTCAA GTGGCTGCGA GAGATTCTCA TCTGTAGGAA TAAATTTCTT
    CTAAAAAATA AACAATCAGA CAGAACTTCC TGTCACTTTC TCTTCCTTTA TGATGTCTCT
    GGAGGTGGAG CTAGTCAGAT GTCCCTTGAC CACGAAGAGA TGATGCGCTG TGCTCCCGGA
    GCCACCCTCA GAAAAGGAAA AGGGAATTCT TCCATGGAAA GTACAGCTGG ATGCAGTGGA
    ACACCCATTT GTCGCCAGGC CCAAACAAAA TTGGAAGTTG CCTTATACAT GTTTTTGTGG
    AGTCCGGATA TTGAGGCAGT CCTTGTTGCA ATGTCCTGTT TCCGTCACCT CTGTGAGGAA
    GCAGATATCA GATGTGGAGT AGATGAAGTT TCAGTTCATA ATTTTTTGCC AAACTACAAC
    ACTTTCATGG AGTTTGCATC TGTTAGCAAT ATGATGTCAA CAGGGAGAGC AGCTCTTCAG
    AAAAGAGTGA TGGCTTTATT AAGACGTATT GAACATCCAA CTGCAGGGAA CACAGAGGCT
    TGGGAGGATA CACATGCAAA ATGGGAACAA GCTACAAAAC TAATCCTTAA CTATCCAAAG
    ACCAAAATGG AAGACGGGCA GGTTACTGAA AGTCTTCATA AGACAATTGT TAAGAGACGA
    ATGTCACATG TTAGTGGGGG AGGCTCCATA GACTTGTCTG ACACAGATTC TCTTCAGGAA
    TGGATCAACA TGACGGGATT CCTCTGTGCC CTCGGAGGAG TGTGCCTGCA GCATCGTAGC
    AATGCAGGAT TAGCAACCTA CAGTCCACCC ATGGGCCCAG TCAATGAGCG TAAGGGCTCC
    ATGATATCTA TGGTATCCAC TGAGGGAAAT GCAGAGACTC CTGTTAGCAA ATTCATAGAT
    CGATTACTGA CTCTGATGGT CTGTAACCAT GAGAAAGTGG GACTACAAAT ACGGACTAAT
    ATTAAAGATC TGGTGGGTTT GGAGTTGAGT CCAGCACTTT ATCCAATGCT ATTTAACAAA
    TTGAAGAATA CCATCAGCAA GTTTTTTGAT TCTCAAGGAC AGGTTTTGTT AACTGAGACC
    AACACACAGT TTGTAGAACA AACCATTGCT ATAATGAAGA ACTTGCTTGA CAATCATACA
    GAAGGGAGCT CAGAACATCT TGGACAAGCT AGTATTGAGA CAATGATGCT AAACCTGGTT
    AGGTATGTGC GTGTACTTGG AAATTTGGTA CATGCAATTC AAATAAAAAC TAAACTCTGT
    CAGTTGGTGG AAGTAATGAT GGAGCGGAGA GATGACCTTT CATTTTGCCA AGAAATGAAA
    TTTAGGAACA AAATGGTGGA ATATCTGACA GACTGGGTTA TGGGAACATC TAATCAAGCA
    ACAGATGAAG ATGTGAAGTG CTTGACAAGA GATTTGGACC AAGCAAGTAT GGAAGCAGTG
    GTCTCTCTTC TTGCTGGGCT TCCGTTACAG CCTGAAGAAG GAGATGGTGT TGAGTTGATG
    GAAGCCAAAT CTCAGTTATT TCTTAAATAT TTCACACTGT TTATGAACCT TCTAAATGAC
    TGTAGTGAAG TTGAAGATGA AAGTGCACAA ACAGGTGGCA GGAAACGTGG GATGTCCCGA
    AGACTGGCTT CGCTGCGGCA CTGCACTGTT CTTGCTATGT CAAATTTACT AAATGCAAAT
    GTGGACAGTG GTCTTATGCA TTCAATAGGC TTGGGCTATC ATAAAGACCT CCAGACAAGA
    GCTACATTTA TGGAAGTCCT CACCAAAATT CTACAGCAGG GAACAGAATT TGATACGTTA
    GCAGAAACGG TGCTGGCAGA TCGGTTTGAG AGGCTGGTGG AGCTAGTTAC AATGATGGGT
    GATCAGGGGG AGCTTCCTAT TGCTATGGCT TTAGCAAATG TGGTGCCTTG TTCTCAGTGG
    GATGAGCTAG CTCGTGTTCT GGTCACACTC TTTGATTCCC GGCACTTGCT CTACCAGCTA
    CTTTGGAATA TGTTTTCAAA GGAAGTTGAA CTGGCAGATT CCATGCAGAC ACTCTTCCGA
    GGAAACAGCT TAGCTAGTAA AATAATGACT TTCTGTTTTA AGGTATATGG TGCTACTTAT
    TTGCAGAAGC TTTTGGAACC TTTATTAAGA ACAGTGATCA CATCCTCTGA ATGGCAGCAT
    GTTAGCTTTG AGGTGGATCC TACAAGACTG GAGCCTACAG AAAGCCTTGA AGAGAACCAG
    CGGAGTCTCT TGCAAATGAC AGAAAAATTT TTTCATGCAA TCATTAATTC TTCTTCGGAA
    TTCCCACCAC AACTTCGCAG TGTGTGCCAT TGTTTGTACC AGGCAACTTG CCACTCTCTA
    CTGAATAAAG CTACCGTAAA AGAAAAAAAG GAAAACAAAA AATCAGTTGT TAGTCAGCGA
    TTTCCTCAGA ACAGCATTGG AGCAGTTGGG AGTGCCATGT TCCTGCGGTT CATCAACCCT
    GCGATCGTCT CCCCGTACGA GGCAGGGATT TTAGATAAAA AGCCTCCGCC CAGAATTGAG
    AGGGGACTGA AACTGATGTC AAAGATACTT CAGAGTATTG CCAACCACGT CCTGTTTACA
    AAAGAAGAAC ATATGAGGCC TTTTAATGAC TTCGTAAAAA GCAATTTTGA TGCAGCTCGA
    AGGTTTTTCC TTGATATTGC ATCTGATTGC CCTGCGAGTG ACACTGTCAA TCACAGCCTG
    TCTTTCATCA GTGATGGCAA TGTTCTTGCT TTGCACCGGT TGTTGTGGAA CAATCAGGAG
    AAAATTGGCC AGTACCTTTC CAGTAATAGG GACCACAAAG CTGTTGGAAG GCGACCTTTT
    GACAAAATGG CTACTCTTCT TGCCTATCTG GGTCCACCAG AGCACAAACC TGTGGCAGAT
    ACACATTGGT CCAGTTTAAA CCTCACCAGT TCGAAATTTG AAGAATTTAT GACAAGGCAT
    CAGGTACATG AAAAAGAAGA GTTCAAAGCA TTGAAAACGT TAAATATTTT CTACCAGGCT
    GGGACATCCA AAGCTGGCAA TCCTGTTTTC TACTACATTG CTCGGCGTTT CAAGACTGGT
    CAGATCAATG GAGATTTATT GATATACCAC GTCCTGCTAA CTCTGAAGCC ATACTACGCA
    AAGCCATATG AAATTGTAGT GGACCTCACA CATGCTGGCC CCAGTAATCG CTTTAAAACA
    GACTTTCTTT CTAAATGGTT TGTAGTTTTT CCAGGCTTTG CTTATGAAAA TGTCTCAGCA
    GTTTTTATTT ATAACTGTAA TTCTTGGGTC AGAGAATACA CCAAATACCA CGAAAGACTC
    TTGACAGGTT TGAAAGGAAG CAAAAGGCTT ATTTTCATAG ACTCTCCAGG AAAACTGGCG
    GAGCACATTG AACATGACCA GCAGAAACTT CCAGCAGCTA CCTTAGCTTT GGAAGAGGAC
    TTGAAAGTAT TTCACAATGC CCTCAAATTG GCTCACAAAG ACACCAAAGT GTCTATTAAA
    GTTGGATCAA CAGCTGTGCA GGTGACATCA GCAGAACGAA CAAGAGTCCT GGGACAGACA
    GTGTTTTTAA ATGATATATA CTACGCCTCT GAAATTGAGG AAATATGTCT TGTTGATGAG
    AATCAGTTTA CATTGACGAT TGCCAACCAG GGAACGCCAC TCACCTTCAT GCATCAGGAG
    TGTGAAGCCA TTGTGAGGTC CATTATTCAT ATACGGACAC GGTGGGAGCT GTCGCAACCA
    GACTCCATCC CACAGCACAC TAAAATTCGC CCTAAGGATG TTCCCGGAAC ACTACTGAAC
    ATAGCATTGC TCAACCTGGG AAGCTCAGAC CCGAGTTTGC GGTCTGCTGC CTATAATCTT
    CTGTGTGCCT TAACCTGTAC CTTTAATTTA AAGATTGAGG GCCAGTTACT GGAAACTTCA
    GGTCTGTGTA TTCCTGCCAA CAACACGCTC TTTATTGTGT CTATTAGTAA GACTCTTGCA
    GCTAATGAGC CACACCTTAC CCTAGAGTTC TTGGAAGAGT GTATTTCAGG ATTTAGCAAA
    TCTAGTATTG AACTGAAACA TCTTTGTCTG GAATATATGA CTCCCTGGCT GTCCAATCTG
    GTCCGATTCT GCAAACACAA CGACGATGCG AAGCGCCAGC GAGTCACTGC CATTCTTGAT
    AAGCTTATAA CAATGACAAT AAATGAAAAA CAGATGTACC CTTCTATTCA GGCTAAAATA
    TGGGGAAGTC TTGGACAGAT TACAGATCTC CTTGACGTAG TGCTGGACAG TTTCATCAAA
    ACTAGTGCCA CGGGGGGCTT AGGATCCATT AAAGCTGAGG TTATGGCAGA CACAGCAGTA
    GCGCTTGCTT CTGGAAATGT AAAATTGGTT TCAAGCAAAG TGATTGGAAG GATGTGCAAA
    ATAATTGACA AGACTTGTCT GTCTCCAACT CCTACACTGG AACAGCACCT GATGTGGGAT
    GATATTGCCA TTCTAGCACG TTACATGCTG ATGCTCTCCT TCAACAATTC TCTGGATGTG
    GCTGCACATC TCCCCTACCT CTTTCATGTG GTTACTTTGT TGGTGGCCAC TGGACCCCTT
    TCTCTCAGAG CTTCCACTCA TGGTCTTGTC ATCAACATCA TTCATTCTCT GTGCACTTGC
    TCACAGCTTC ATTTTAGTGA GGAGACCAAG CAAGTTTTAA GACTGAGCTT GACTGAATTT
    TCTCTGCCTA AATTTTATTT GCTGTTTGGA ATCAGCAAAG TGAAATCAGC TGCAGTCATA
    GCATTCCGCT CCAGCTATCG AGACAGGTCA TTTTCACCTG GCTCCTACGA GAGGGAGACT
    TTTGCACTGA CCTCCTTGGA AACTGTTACT GAAGCTTTGC TGGAGATCAT GGAGGCTTGT
    ATGAGAGATA TTCCAACATG CAAGTGGCTG GACCAGTGGA CCGAACTAGC ACAAAAGTTT
    GCATTCCAGT ACAATCCATC CCTGCAGCCA AGAGCACTCG TTGTCTTTGG CTGTATAAGT
    AAGCGTGTCT CTCATGGACA AATAAAACAA ATAATCCGAA TTCTTAGCAA GGGGCTTGAG
    AGCTGTTTAA AAGGCCCTGA TAATTACAAT AGCCAAGTTC TAATAGAAGC TACGGTTATA
    GCACTCACCA AACTGCAGCC TCTTCTTAAC AAGGACTCCC CCATGCACAA GGCCCTCTTC
    TGGGTAGCCA TGGCAGTACT GCAGTTGGAT GAAGTCAACC TATACTCAGC AGGTACAGCA
    CTGCTTGAAC AGAATCTGCA TACCTTGGAT AGCCTTCGAG TGTTCAATGA TAAGAGCCCA
    GAAGAGGTGT TCATGGAGAT CAGGAGACCA CTTGAATGGC ACTGCAAACA GATGGATCAT
    TTTGTTGGGC TAAATTTCAA CTCCAACTTC AACTTTGCAC TAGTGGGGCA CCTCCTGAAA
    GGGTACAGGC ATCCTTCACC TACCACTGTA GCAAGAACAG TAAGGATTCT ACATACACTA
    CTAGCTCTGG TTAACAAACA CAGGAACTGT GACAAGTTTG AGGTGAACAC CCAGAGCGTG
    GCTTACCTAG CAGCTTTGCT GACAGTGTCT GAGGAAGTTC GAAGTCGCTG CAGCCTAAAA
    CATAGGAAGT CTCTCTTGCT GACAGATGTT GCAATGGAAA ATGTTCCTAT GGATACATAT
    CCCATACATC ACAGTGACAC TACCTATAGG ACACTAAAGG AAAATCAACC ATGGTCATCA
    CCAAAGGGAT CAGACAGACA CCTGGCTGCC AGTTACCCGA CAGTGGGACA GATAAGCCCT
    CGAACACGAA AGTCCATGAG TTTGGATATG GGGCAGCCTT CACAAGCGAA CACTAAAAAG
    CTTCTAGGTA CAAGGAAAAG TTTTGACCAT TTGATATCGG ACACAAAGGC TCCTAAAAGG
    CAAGACATGG AATCTGGAAT CACAACGCCT CCCAAAATGA GGAGAGTAGC CGAAAATGAT
    TATGAAATGG AAACCCAGAG AATATCACCG CAGCAGCACC CCCATCTCCG GAAAGTTTCT
    GTTTCTGAGT CAAATGTCCT CCTGGATGAA GAAGTTCTGA CTGATCCAAA AATTCAAGCA
    CTGCTGCTTA CAGTTCTTGC CACGTTAGTG AAGTACACTA CTGATGAATT TGACCAGCGG
    ATTCTGTATG AGTATTTAGC AGAAGCCAGT GTTGTCTTCC CAAAGGTCTT TCCTGTTGTG
    CATAATTTAT TGGATTCCAA GATCAATACC CTCTTGTCGC TGTGTCAAGA TCCAAATTTG
    TTGAATCCAA TTCATGGGAT TGTTCAGAGT GTTGTCTACC ATGAGGAGTC TCCACCCCAA
    TACCAAACTT CTTATCTACA AAGTTTTGGA TTTAATGGGT TGTGGAGGTT TGCTGGCCCA
    TTTTCCAAGC AAACACAAAT CCCAGACTAT GCTGAGCTCA TAGTGAAGTT TCTTGATGCC
    TTGATTGACA CGTATTTGCC TGGAATTGAT GAGGAAACCA GTGAAGAATC TCTCCTGACA
    CCCACGTCTC CATATCCTCC CGCAGTGCAG AGCCAGCTTA GCATTACAGC TAACCTTAAC
    CTTTCCAATT CCATGACCTC GCTCGCAACT TCCCAGCACT CCCCAGCTTC TCTGCCTTGC
    TCTAAATCTG CAGTTTTCAT GCAGCTTCCT CATCAAGGGA TCGACAAGGA GAACGTGGAG
    CTCTCGCCCA CCACTGGCCA CACCAACAGC GGGAGGATGC GCCACGGCTC CGCCAGCCAG
    GTGCAAAAGC AGCGAAGTGC TGGCAGCTTG AAACGTCACA GCATTAAGAA GATTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015151342.1
    CDS77..8416
    Translation

    Target ORF information:

    RefSeq Version XM_015295856.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofibromin 1 (NF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015295856.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGTGGCCTG GGGTAAGAAG GGGCACACAG CAACCGAAGG ATACGATGCG ACTAGACGAG 
    ACTATGCTGG TAAAACAGTT GCTGCCAGAA ATCTGCCACT TCATCCACAC TTACCGTGAA
    GGGAACCAGC ATGCAGCTGA ACTACGCAAT TCTGCCTCTG GGGTTCTTTT TTCTCTTAGC
    TGCAATAACT TTAACGCTGT GTTCAGCCGC ATTTCCACCA GATTGCAGGA ACTGACTGTT
    TGCTCAGAAG ATAATGCCGA TGTTCACGAC ATTGAACTTC TACAGTACAT CAGTGTGGAT
    TGTTCAAAGC TAAAAAGACT CTTGCAAGAG ACTGTATTCA AGTTTAAAGC CCTTAAGAAA
    GTAGCTCAGT TAGCTGTTAT CAACAGTCTT GAAAAGGCAT TTTGGAACTG GGTGGAAAAC
    TATCCTGATG AATTCACAAA GCTGTATCAA ACACCACAGA CTGATATGGC CGATTGTGCA
    GAGAAGTTGT TTGACTTAGT GGATGGCTTT GCAGAGAGCA CAAAACGTAA AGCAGCGGTG
    TGGCCACTAC AAATCATACT CCTGGTCCTG TGTCCAGAGA TAATTCAAGA TATAGCAAAG
    GATGTAGTAG AGGAAACTAA AATGAACAAG AAACTGTTCC TGGACAATCT CAGGAAAGCA
    CTTGCAGGCC ACAGTGGGAG CAGGCAGCTG ACTGAAAGCG CTGCTATTGC TTGTGTTAAA
    CTGTGCAAAG CATCTACCTA CATCAACTGG GAGGATAATT CTGTCATTTT TCTACTAGTG
    CAGTCCATGG TAGTAGATCT TAAGAATTTG CTGTTTAATC CGAGCAAACC TTTTTCAAGA
    AGCAATCAGA ATGCAGATGT AGACCTTATG ATCGACTGCT TAGTTTCATG CTTCCGCATA
    AACCCGCATA ATAACCAACA CTTTAAGATC TGCTTGGCAC AGAGTTCCCC ATCAACATTT
    CATTATGTAC TAGTAAATTC ACTCCACCGA ATAATCACAA ATTCTGCATT GGACTGGTGG
    CCCAGGATTG ATGCTGTGTA CTGCCATTCC GTGGAGCTTC GCAGCATGTT TAGTGAGACA
    CTTCACAAAG CTATTCAGGG CTGTGGGGCA CATCCAGCAA TTCGCATGAC TCCGATGCCA
    ACCAGTCACT GTTTTGCATG GCAGTGGGCC TCTGTTGCTA GTCTGACATT TAAAGAGAAA
    ATGACAAGCC TTAAATTTAA AGAAAAGCCT ACTGACTTGG AAACCAAAAG CTACAAGTTC
    CTGCTGTTAT CCTTGGTAAA ACTGATCCAT GCTGATCCAA AGCTTTTGCT GTGTAATCCA
    AGGAAGCAAG GGCCTGAGAC ACAAGGCAGC ACTGCTGAAT TAATTACAGG CCTTGTACAA
    CTCGTTCCAC AATCCAGCAT GCCAGATATA GCACAAGAAG CCATGGAGGC CTTGCTAGTT
    CTTCACCAGT TAGACAGCAT TGACTTATGG AATCCTGATG CACCTATAGA AACTTTCTGG
    GAAATTAGTT CACAAATGCT TTTTTATATC TGCAAGAAAT TAACCAGTCA TCAGATGCTC
    AGCAGTACAG AAATCCTCAA GTGGCTGCGA GAGATTCTCA TCTGTAGGAA TAAATTTCTT
    CTAAAAAATA AACAATCAGA CAGAACTTCC TGTCACTTTC TCTTCCTTTA TGATGTCTCT
    GGAGGTGGAG CTAGTCAGAT GTCCCTTGAC CACGAAGAGA TGATGCGCTG TGCTCCCGGA
    GCCACCCTCA GAAAAGGAAA AGGGAATTCT TCCATGGAAA GTACAGCTGG ATGCAGTGGA
    ACACCCATTT GTCGCCAGGC CCAAACAAAA TTGGAAGTTG CCTTATACAT GTTTTTGTGG
    AGTCCGGATA TTGAGGCAGT CCTTGTTGCA ATGTCCTGTT TCCGTCACCT CTGTGAGGAA
    GCAGATATCA GATGTGGAGT AGATGAAGTT TCAGTTCATA ATTTTTTGCC AAACTACAAC
    ACTTTCATGG AGTTTGCATC TGTTAGCAAT ATGATGTCAA CAGGGAGAGC AGCTCTTCAG
    AAAAGAGTGA TGGCTTTATT AAGACGTATT GAACATCCAA CTGCAGGGAA CACAGAGGCT
    TGGGAGGATA CACATGCAAA ATGGGAACAA GCTACAAAAC TAATCCTTAA CTATCCAAAG
    ACCAAAATGG AAGACGGGCA GGTTACTGAA AGTCTTCATA AGACAATTGT TAAGAGACGA
    ATGTCACATG TTAGTGGGGG AGGCTCCATA GACTTGTCTG ACACAGATTC TCTTCAGGAA
    TGGATCAACA TGACGGGATT CCTCTGTGCC CTCGGAGGAG TGTGCCTGCA GCATCGTAGC
    AATGCAGGAT TAGCAACCTA CAGTCCACCC ATGGGCCCAG TCAATGAGCG TAAGGGCTCC
    ATGATATCTA TGGTATCCAC TGAGGGAAAT GCAGAGACTC CTGTTAGCAA ATTCATAGAT
    CGATTACTGA CTCTGATGGT CTGTAACCAT GAGAAAGTGG GACTACAAAT ACGGACTAAT
    ATTAAAGATC TGGTGGGTTT GGAGTTGAGT CCAGCACTTT ATCCAATGCT ATTTAACAAA
    TTGAAGAATA CCATCAGCAA GTTTTTTGAT TCTCAAGGAC AGGTTTTGTT AACTGAGACC
    AACACACAGT TTGTAGAACA AACCATTGCT ATAATGAAGA ACTTGCTTGA CAATCATACA
    GAAGGGAGCT CAGAACATCT TGGACAAGCT AGTATTGAGA CAATGATGCT AAACCTGGTT
    AGGTATGTGC GTGTACTTGG AAATTTGGTA CATGCAATTC AAATAAAAAC TAAACTCTGT
    CAGTTGGTGG AAGTAATGAT GGAGCGGAGA GATGACCTTT CATTTTGCCA AGAAATGAAA
    TTTAGGAACA AAATGGTGGA ATATCTGACA GACTGGGTTA TGGGAACATC TAATCAAGCA
    ACAGATGAAG ATGTGAAGTG CTTGACAAGA GATTTGGACC AAGCAAGTAT GGAAGCAGTG
    GTCTCTCTTC TTGCTGGGCT TCCGTTACAG CCTGAAGAAG GAGATGGTGT TGAGTTGATG
    GAAGCCAAAT CTCAGTTATT TCTTAAATAT TTCACACTGT TTATGAACCT TCTAAATGAC
    TGTAGTGAAG TTGAAGATGA AAGTGCACAA ACAGGTGGCA GGAAACGTGG GATGTCCCGA
    AGACTGGCTT CGCTGCGGCA CTGCACTGTT CTTGCTATGT CAAATTTACT AAATGCAAAT
    GTGGACAGTG GTCTTATGCA TTCAATAGGC TTGGGCTATC ATAAAGACCT CCAGACAAGA
    GCTACATTTA TGGAAGTCCT CACCAAAATT CTACAGCAGG GAACAGAATT TGATACGTTA
    GCAGAAACGG TGCTGGCAGA TCGGTTTGAG AGGCTGGTGG AGCTAGTTAC AATGATGGGT
    GATCAGGGGG AGCTTCCTAT TGCTATGGCT TTAGCAAATG TGGTGCCTTG TTCTCAGTGG
    GATGAGCTAG CTCGTGTTCT GGTCACACTC TTTGATTCCC GGCACTTGCT CTACCAGCTA
    CTTTGGAATA TGTTTTCAAA GGAAGTTGAA CTGGCAGATT CCATGCAGAC ACTCTTCCGA
    GGAAACAGCT TAGCTAGTAA AATAATGACT TTCTGTTTTA AGGTATATGG TGCTACTTAT
    TTGCAGAAGC TTTTGGAACC TTTATTAAGA ACAGTGATCA CATCCTCTGA ATGGCAGCAT
    GTTAGCTTTG AGGTGGATCC TACAAGACTG GAGCCTACAG AAAGCCTTGA AGAGAACCAG
    CGGAGTCTCT TGCAAATGAC AGAAAAATTT TTTCATGCAA TCATTAATTC TTCTTCGGAA
    TTCCCACCAC AACTTCGCAG TGTGTGCCAT TGTTTGTACC AGGCAACTTG CCACTCTCTA
    CTGAATAAAG CTACCGTAAA AGAAAAAAAG GAAAACAAAA AATCAGTTGT TAGTCAGCGA
    TTTCCTCAGA ACAGCATTGG AGCAGTTGGG AGTGCCATGT TCCTGCGGTT CATCAACCCT
    GCGATCGTCT CCCCGTACGA GGCAGGGATT TTAGATAAAA AGCCTCCGCC CAGAATTGAG
    AGGGGACTGA AACTGATGTC AAAGATACTT CAGAGTATTG CCAACCACGT CCTGTTTACA
    AAAGAAGAAC ATATGAGGCC TTTTAATGAC TTCGTAAAAA GCAATTTTGA TGCAGCTCGA
    AGGTTTTTCC TTGATATTGC ATCTGATTGC CCTGCGAGTG ACACTGTCAA TCACAGCCTG
    TCTTTCATCA GTGATGGCAA TGTTCTTGCT TTGCACCGGT TGTTGTGGAA CAATCAGGAG
    AAAATTGGCC AGTACCTTTC CAGTAATAGG GACCACAAAG CTGTTGGAAG GCGACCTTTT
    GACAAAATGG CTACTCTTCT TGCCTATCTG GGTCCACCAG AGCACAAACC TGTGGCAGAT
    ACACATTGGT CCAGTTTAAA CCTCACCAGT TCGAAATTTG AAGAATTTAT GACAAGGCAT
    CAGGTACATG AAAAAGAAGA GTTCAAAGCA TTGAAAACGT TAAATATTTT CTACCAGGCT
    GGGACATCCA AAGCTGGCAA TCCTGTTTTC TACTACATTG CTCGGCGTTT CAAGACTGGT
    CAGATCAATG GAGATTTATT GATATACCAC GTCCTGCTAA CTCTGAAGCC ATACTACGCA
    AAGCCATATG AAATTGTAGT GGACCTCACA CATGCTGGCC CCAGTAATCG CTTTAAAACA
    GACTTTCTTT CTAAATGGTT TGTAGTTTTT CCAGGCTTTG CTTATGAAAA TGTCTCAGCA
    GTTTTTATTT ATAACTGTAA TTCTTGGGTC AGAGAATACA CCAAATACCA CGAAAGACTC
    TTGACAGGTT TGAAAGGAAG CAAAAGGCTT ATTTTCATAG ACTCTCCAGG AAAACTGGCG
    GAGCACATTG AACATGACCA GCAGAAACTT CCAGCAGCTA CCTTAGCTTT GGAAGAGGAC
    TTGAAAGTAT TTCACAATGC CCTCAAATTG GCTCACAAAG ACACCAAAGT GTCTATTAAA
    GTTGGATCAA CAGCTGTGCA GGTGACATCA GCAGAACGAA CAAGAGTCCT GGGACAGACA
    GTGTTTTTAA ATGATATATA CTACGCCTCT GAAATTGAGG AAATATGTCT TGTTGATGAG
    AATCAGTTTA CATTGACGAT TGCCAACCAG GGAACGCCAC TCACCTTCAT GCATCAGGAG
    TGTGAAGCCA TTGTGAGGTC CATTATTCAT ATACGGACAC GGTGGGAGCT GTCGCAACCA
    GACTCCATCC CACAGCACAC TAAAATTCGC CCTAAGGATG TTCCCGGAAC ACTACTGAAC
    ATAGCATTGC TCAACCTGGG AAGCTCAGAC CCGAGTTTGC GGTCTGCTGC CTATAATCTT
    CTGTGTGCCT TAACCTGTAC CTTTAATTTA AAGATTGAGG GCCAGTTACT GGAAACTTCA
    GGTCTGTGTA TTCCTGCCAA CAACACGCTC TTTATTGTGT CTATTAGTAA GACTCTTGCA
    GCTAATGAGC CACACCTTAC CCTAGAGTTC TTGGAAGAGT GTATTTCAGG ATTTAGCAAA
    TCTAGTATTG AACTGAAACA TCTTTGTCTG GAATATATGA CTCCCTGGCT GTCCAATCTG
    GTCCGATTCT GCAAACACAA CGACGATGCG AAGCGCCAGC GAGTCACTGC CATTCTTGAT
    AAGCTTATAA CAATGACAAT AAATGAAAAA CAGATGTACC CTTCTATTCA GGCTAAAATA
    TGGGGAAGTC TTGGACAGAT TACAGATCTC CTTGACGTAG TGCTGGACAG TTTCATCAAA
    ACTAGTGCCA CGGGGGGCTT AGGATCCATT AAAGCTGAGG TTATGGCAGA CACAGCAGTA
    GCGCTTGCTT CTGGAAATGT AAAATTGGTT TCAAGCAAAG TGATTGGAAG GATGTGCAAA
    ATAATTGACA AGACTTGTCT GTCTCCAACT CCTACACTGG AACAGCACCT GATGTGGGAT
    GATATTGCCA TTCTAGCACG TTACATGCTG ATGCTCTCCT TCAACAATTC TCTGGATGTG
    GCTGCACATC TCCCCTACCT CTTTCATGTG GTTACTTTGT TGGTGGCCAC TGGACCCCTT
    TCTCTCAGAG CTTCCACTCA TGGTCTTGTC ATCAACATCA TTCATTCTCT GTGCACTTGC
    TCACAGCTTC ATTTTAGTGA GGAGACCAAG CAAGTTTTAA GACTGAGCTT GACTGAATTT
    TCTCTGCCTA AATTTTATTT GCTGTTTGGA ATCAGCAAAG TGAAATCAGC TGCAGTCATA
    GCATTCCGCT CCAGCTATCG AGACAGGTCA TTTTCACCTG GCTCCTACGA GAGGGAGACT
    TTTGCACTGA CCTCCTTGGA AACTGTTACT GAAGCTTTGC TGGAGATCAT GGAGGCTTGT
    ATGAGAGATA TTCCAACATG CAAGTGGCTG GACCAGTGGA CCGAACTAGC ACAAAAGTTT
    GCATTCCAGT ACAATCCATC CCTGCAGCCA AGAGCACTCG TTGTCTTTGG CTGTATAAGT
    AAGCGTGTCT CTCATGGACA AATAAAACAA ATAATCCGAA TTCTTAGCAA GGGGCTTGAG
    AGCTGTTTAA AAGGCCCTGA TAATTACAAT AGCCAAGTTC TAATAGAAGC TACGGTTATA
    GCACTCACCA AACTGCAGCC TCTTCTTAAC AAGGACTCCC CCATGCACAA GGCCCTCTTC
    TGGGTAGCCA TGGCAGTACT GCAGTTGGAT GAAGTCAACC TATACTCAGC AGGTACAGCA
    CTGCTTGAAC AGAATCTGCA TACCTTGGAT AGCCTTCGAG TGTTCAATGA TAAGAGCCCA
    GAAGAGGTGT TCATGGAGAT CAGGAGACCA CTTGAATGGC ACTGCAAACA GATGGATCAT
    TTTGTTGGGC TAAATTTCAA CTCCAACTTC AACTTTGCAC TAGTGGGGCA CCTCCTGAAA
    GGGTACAGGC ATCCTTCACC TACCACTGTA GCAAGAACAG TAAGGATTCT ACATACACTA
    CTAGCTCTGG TTAACAAACA CAGGAACTGT GACAAGTTTG AGGTGAACAC CCAGAGCGTG
    GCTTACCTAG CAGCTTTGCT GACAGTGTCT GAGGAAGTTC GAAGTCGCTG CAGCCTAAAA
    CATAGGAAGT CTCTCTTGCT GACAGATGTT GCAATGGAAA ATGTTCCTAT GGATACATAT
    CCCATACATC ACAGTGACAC TACCTATAGG ACACTAAAGG AAAATCAACC ATGGTCATCA
    CCAAAGGGAT CAGACAGACA CCTGGCTGCC AGTTACCCGA CAGTGGGACA GATAAGCCCT
    CGAACACGAA AGTCCATGAG TTTGGATATG GGGCAGCCTT CACAAGCGAA CACTAAAAAG
    CTTCTAGGTA CAAGGAAAAG TTTTGACCAT TTGATATCGG ACACAAAGGC TCCTAAAAGG
    CAAGACATGG AATCTGGAAT CACAACGCCT CCCAAAATGA GGAGAGTAGC CGAAAATGAT
    TATGAAATGG AAACCCAGAG AATATCACCG CAGCAGCACC CCCATCTCCG GAAAGTTTCT
    GTTTCTGAGT CAAATGTCCT CCTGGATGAA GAAGTTCTGA CTGATCCAAA AATTCAAGCA
    CTGCTGCTTA CAGTTCTTGC CACGTTAGTG AAGTACACTA CTGATGAATT TGACCAGCGG
    ATTCTGTATG AGTATTTAGC AGAAGCCAGT GTTGTCTTCC CAAAGGTCTT TCCTGTTGTG
    CATAATTTAT TGGATTCCAA GATCAATACC CTCTTGTCGC TGTGTCAAGA TCCAAATTTG
    TTGAATCCAA TTCATGGGAT TGTTCAGAGT GTTGTCTACC ATGAGGAGTC TCCACCCCAA
    TACCAAACTT CTTATCTACA AAGTTTTGGA TTTAATGGGT TGTGGAGGTT TGCTGGCCCA
    TTTTCCAAGC AAACACAAAT CCCAGACTAT GCTGAGCTCA TAGTGAAGTT TCTTGATGCC
    TTGATTGACA CGTATTTGCC TGGAATTGAT GAGGAAACCA GTGAAGAATC TCTCCTGACA
    CCCACGTCTC CATATCCTCC CGCAGTGCAG AGCCAGCTTA GCATTACAGC TAACCTTAAC
    CTTTCCAATT CCATGACCTC GCTCGCAACT TCCCAGCACT CCCCAGCTTC TCTGCCTTGC
    TCTAAATCTG CAGTTTTCAT GCAGCTTCCT CATCAAGGGA TCGACAAGGA GAACGTGGAG
    CTCTCGCCCA CCACTGGCCA CACCAACAGC GGGAGGATGC GCCACGGCTC CGCCAGCCAG
    GTGCAAAAGC AGCGAAGTGC TGGCAGCTTG AAACGTCACA GCATTAAGAA GATTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.