Nf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nf1
Entrez Gene ID	24592
Full Name	neurofibromin 1
General protein information	Preferred Names neurofibromin 1 Names neurofibromin Neurofibromatosis type 1 neurofibromatosis 1 neurofibromatosis-related protein NF-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q25
Summary	mutations in gene are associated with neurofibromatosis type 1 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012609.1	NP_036741.1	neurofibromin

Pathways from BioSystems

Homologies

Homo sapiens (human)	NF1	NP_001035957.1
Rattus norvegicus (Norway rat)	Nf1	NP_036741.1
Danio rerio (zebrafish)	nf1b	XP_692937.4
Drosophila melanogaster (fruit fly)	Nf1	NP_733132.2
	NF1	XP_002800429.1
Canis lupus familiaris (dog)	NF1	XP_537738.3
Mus musculus (house mouse)	Nf1	NP_035027.1
Gallus gallus (chicken)	NF1	XP_004946843.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003140	XP_312829.4
Xenopus tropicalis (tropical clawed frog)	nf1	XP_002936865.2
Pan troglodytes (chimpanzee)	NF1	XP_511395.2

Gene Ontology
Bibliography

Related articles in PubMed

Neurofibromin Regulates Seizure Attacks in the Rat Pilocarpine-Induced Model of Epilepsy.
Ren M, Li K, Wang D, Guo J, Li J, Yang G, Long X, Shen W, Hu R, Wang X, Zeng K
Molecular neurobiology53(9)6069-6077(2016 Nov)

Clinicopathologic implications of NF1 gene alterations in diffuse gliomas.
Vizca?no MA, Shah S, Eberhart CG, Rodriguez FJ
Human pathology46(9)1323-30(2015 Sep)

Social learning and amygdala disruptions in Nf1 mice are rescued by blocking p21-activated kinase.
Molosh AI, Johnson PL, Spence JP, Arendt D, Federici LM, Bernabe C, Janasik SP, Segu ZM, Khanna R, Goswami C, Zhu W, Park SJ, Li L, Mechref YS, Clapp DW, Shekhar A
Nature neuroscience17(11)1583-90(2014 Nov)

Identification of genes important for cutaneous function revealed by a large scale reverse genetic screen in the mouse.
DiTommaso T, Jones LK, Cottle DL, , Gerdin AK, Vancollie VE, Watt FM, Ramirez-Solis R, Bradley A, Steel KP, Sundberg JP, White JK, Smyth IM
PLoS genetics10(10)e1004705(2014 Oct)

Neurofibromin is the major ras inactivator in dendritic spines.
Oliveira AF, Yasuda R
The Journal of neuroscience : the official journal of the Society for Neuroscience34(3)776-83(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ras inactivation by neurofibromin is required for sustained spine enlargement associated with long term potentiation (LTP) and maintenance of spine density.
Title: Neurofibromin is the major ras inactivator in dendritic spines.

Immunoprecipitation from rat brain shows that neurofibromin forms a complex with VCP and p47. The LRD domain is required.
Title: Valosin-containing protein and neurofibromin interact to regulate dendritic spine density.

association of neurofibromin and CRMP-2 is essential for neuronal cell differentiation
Title: Neurofibromatosis type 1 (NF1) tumor suppressor, neurofibromin, regulates the neuronal differentiation of PC12 cells via its associating protein, CRMP-2.

Loss of NF1 function may therefore lead to dysplastic bone growth, thereby causing the debilitating osseous symptoms of NF1
Title: NF1 tumor suppressor protein and mRNA in skeletal tissues of developing and adult normal mouse and NF1-deficient embryos.

The following Nf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12357	NM_012609.1 Latest version!	Rattus norvegicus neurofibromin 1 (Nf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nf1 ( NM_012609.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036741.1 Rattus norvegicus neurofibromin 1 (Nf1), mRNA.

Price & Availability↑

CloneID	ORa12357
Clone ID Related Accession (Same CDS sequence)	NM_012609.1
Accession Version	NM_012609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Rattus norvegicus(Norway rat)
Product	neurofibromin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D45201.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D45201.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGCACGGA GCACAACAAG 
GAGTGTCTGA TCAACATTTC CAAGTACAAG TTTTCTTTGG TCATCAGTGG ACTCACCACC 
ATTCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT 
CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC 
ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TACCAGAAAT CTGCCATTTT 
CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCTTCTGGG 
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGAAT TTCTACAAGG 
TTACAGGAGT TGACTGTCTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA 
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA 
TTTAAAGCCC TGAAGAAGGT GGCACAATTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT 
TGGAACTGGG TGGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA 
GATATGGCTG AGTGTGCAGG AAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC 
AAACGTAAAG CAGCAGTTTG GCCACTACAG ATCATTCTCC TCATTCTGTG TCCAGAAATA 
ATCCAGGACA TATCCAGGGA TGTGGTCGAT GAGAACAACA CAAACAAGAA ACTGTTTCTG 
GACAGTTTAC GGAAAGCACT TGCTGGCCAT GGCGGAAGCA GACAGCTGAC TGAAAGTGCT 
GCCATCGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGACAACTCT 
GTCATTTTCC TACTGGTTCA GTCCATGGTG GTTGATCTGA AGAACCTGCT TTTTAATCCA 
AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT 
GTCTCTTGCT TTCGGATCAG CCCTCACAAC AACCAACACT TTAAGATCTG TCTGGCTCAG 
AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TCCACCGCAT TATCACCAAT 
TCTGCATGGG ATTGGTGGCC TAAGATTGAC GCTGTATATT GTCACTCAGT TGAACTTCGA 
AATATGTTTG GTGAAACCCT TCATAAAGCA GTGCAAGGCT GTGGAGCACA CCCAGCATTA 
CGGATGGCAC CAAGTCTTAC CTTTAAGGAA AAGGTAACAA GCCTTAAGTT TAAAGAAAAG 
CCTACGGACC TAGAGGCAAG GAGCTACAAG TATCTTCTTT TGTCTATGGT GAAACTGATT 
CATGCAGACC CAAAGCTTTT GCTTTGTAAT CCAAGAAAAC AGGGCCCTGA AACTCAAGGT 
AGTACAGCAG AACTGATTAC AGGGCTCGTA CAGCTGGTTC CTCAGTCACA CATGCCAGAG 
GTCGCTCAGG AGGCTATGGA GGCTTTGTTG GTTCTTCATC AGTTAGACAG CATTGATCTG 
TGGAATCCTG ATGCTCCTGT AGAAACGTTT TGGGAAATTA GTTCACAAAT GCTTTTTTAC 
ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTA 
CGGGAAATTC TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT 
TCCTGTCATT CTCTCTACCT TTATGGAGTA GGATGTGACC TGCCTGCTAG TGGAAATGTC 
ACTCAGATGT CAGTTGATCA TGAGGAGTCA CTACGTACCT GTGCTCCTGG AGCCTCTCTG 
CGGAAAGGAA GAGGGAATTC CTCCATGGAT AGCACAGCAG GGTGCAGTGG AACCCCACCT 
ATATGCCGAC AAGCCCAGAC CAAGCTAGAA GTGGCCTTGT ACATGTTTCT GTGGAGTCCT 
GACACGGAAG TTGTTCTGGT TGCTATGTCC TGTTTCCGCC ACCTCTGTGA AGAAGCAGAC 
ATCCGATGTG GTGTGGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA CAACACATTC 
ATGGAATTTG CCTCAGTCAG CAATATGTTG TCAACAGGAA GAGCGGCACT TCAGAAAAGA 
GTGATGGCCC TGCTAAGGCG CATTGAGCAC CCTACTGCAG GAAACACTGA GGCCTGGGAA 
GATACACATG CAAAGTGGGA ACAGGCTACA AAACTAATCC TTAACTACCC AAAGGCCAAA 
ATGGAAGATG GCCAGGCTGC AGAAAGTCTT CATAAGACCA TTGTTAAGAG ACGGATGTCC 
CATGTCAGTG GAGGAGGATC CATAGACTTG TCTGACACAG ACTCCCTGCA GGAATGGATC 
AACATGACTG GCTTCCTCTG TGCCCTTGGT GGGGTGTGCC TGCAACAGAG AAGCAGCTCT 
GGCCTAGCAA CATACAGCCC ACCTATGGGC CCTGTCAGTG AACGCAAAGG GTCTATGATT 
TCTGTAATGT CTTCTGAAGG GAATGTTGAT TCACCTGTCA GCAGATTTAT GGACCGGCTT 
CTGTCCTTGA TGGTGTGTAA CCATGAGAAA GTGGGGCTTC AGATACGGAC CAATGTTAAG 
GACCTGGTGG GTCTGGAGCT GAGTCCTGCT CTGTATCCAA TGCTGTTCAA CAAACTGAAG 
AGTGCCATCA GCAAGTTTTT TGACTCTCAA GGACAGGTAT TATTAACTGA CAGCAATACT 
CAGTTTGTAG AGCAAACCAT AGCTATAATG AAGAACCTAC TGGATAATCA TACTGAAGGC 
AGCTCTGAGC ATCTGGGACA AGCTAGCATT GAGACAATGA TGCTAAATCT GGTCAGATAT 
GTTCGTGTTC TTGGGAATAT GGTTCATGCA ATTCAAATAA AAACGAAATT GTGTCAGTTA 
GTTGAAGTCA TGATGGCAAG AAGAGACGAC CTCTCGTTTT GTCAAGAGAT GAAATTTCGG 
AATAAGATGG TGGAGTACCT AACAGACTGG GTTATGGGCA CTTCAAACCA AGCCGCAGAT 
GATGATGTAA AGTGTCTCAC AAGAGATCTA GACCAAGCAA GCATGGAGGC AGTAGTCTCG 
CTCCTCGCTG GTCTTCCACT GCAGCCTGAA GAAGGAGACG GAGTGGAGCT GATGGAGGCC 
AAGTCACAGC TATTCCTTAA GTACTTCACA TTATTTATGA ACCTTTTGAA TGACTGTAGT 
GAAGTTGAAG ATGAAAATGC ACAAACAGGT GGCAGGAAAC GTGGCATGTC TCGGAGGCTG 
GCATCCCTGA GGCACTGTAC AGTGCTTGCA ATGTCAAACT TACTCAATGC TAATGTGGAC 
AGTGGACTCA TGCACTCCAT AGGCTTAGGT TATCATAAAG ATCTTCAGAC AAGAGCTACA 
TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGAACAG AATTTGATAC ACTTGCTGAA 
ACCGTGTTGG CAGATCGGTT CGAGAGACTT GTGGAACTGG TCACAATGAT GGGAGACCAG 
GGAGAGCTCC CTATAGCTAT GGCTCTGGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAG 
CTGGCTCGAG TTCTGGTCAC GCTGTTTGAT TCTCGGCATT TGCTCTACCA GCTGCTCTGG 
AACATGTTTT CTAAGGAGGT AGAGTTGGCA GACTCCATGC AGACTCTCTT TCGAGGCAAC 
AGCTTGGCCA GTAAGATAAT GACGTTCTGC TTCAAGGTGT ACGGTGCTAC TTACCTACAA 
AAGCTCTTGG ACCCCTTGTT ACGAATCATT ATCACATCCT CCGATTGGCA GCATGTTAGC 
TTTGAAGTGG ATCCTACCAG GTTAGAGCCC TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC 
CTCCTTCAGA TGACAGAGAA GTTCTTCCAT GCCATCATCA GTTCTTCCTC AGAGTTCCCC 
TCGCAGCTTC GAAGTGTCTG CCATTGTTTG TACCAGGTGG TTAGCCAGCG CTTCCCTCAG 
AACAGCATCG GTGCCGTCGG AAGTGCCATG TTCCTGAGGT TCATCAACCC TGCCATTGTC 
TCACCGTATG AAGCAGGGAT TTTAGATAAA AAGCCACCAC CTAGAATTGA AAGGGGCTTG 
AAGTTAATGT CAAAGGTCCT TCAGAGCATT GCCAATCATG TACTGTTCAC AAAGGAAGAG 
CACATGAGGC CTTTTAATGA TTTTGTGAAA AGCAACTTCG ACTTGGCACG GAGGTTTTTC 
TTAGATATAG CATCAGATTG TCCCACAAGT GACGCAGTAA ACCATAGTCT TTCCTTCATC 
AGTGATGGCA ATGTGCTCGC TTTACATCGG CTGCTTTGGA ATAATCAGGA GAAAATTGGC 
CAGTATCTTT CCAGCAACAG GGATCATAAA GCTGTTGGAA GACGACCTTT CGATAAGATG 
GCCACACTTC TTGCATATCT GGGTCCTCCG GAGCACAAGC CTGTGGCAGA TACACACTGG 
TCCAGCCTTA ACCTTACCAG TTCAAAGTTT GAGGAATTTA TGACCAGGCA TCAGGTTCAT 
GAGAAGGAAG AGTTCAAGGC CTTGAAAACA TTAAGCATCT TCTACCAAGC TGGCACATCC 
AAAGCTGGGA ATCCTATTTT CTATTATGTT GCACGGAGGT TCAAAACTGG CCAAATCAAT 
GGGGATTTGC TGATATACCA TGTCTTGCTG ACTTTAAAGC CATATTATGC AAAGCCATAT 
GAAATTGTAG TGGACCTTAC CCATACTGGG CCTAGCAATC GCTTTAAAAC AGACTTCCTC 
TCTAAGTGGT TTGTTGTTTT TCCTGGCTTT GCTTATGACA ACGTGTCTGC AGTCTATATC 
TATAACTGTA ACTCCTGGGT CAGGGAGTAC ACCAAATACC ATGAGCGGCT GCTGACTGGC 
CTCAAGGGCA GCAAAAGGCT CATTTTCATC GACTGTCCTG GGAAGCTGGC TGAGCACATA 
GAGCACGAGC AACAGAAGCT CCCTGCTGCT ACCTTGGCAC TGGAAGAGGA TCTAAAGGTT 
TTCCACAATG CGCTCAAGCT AGCTCACAAG GACACCAAAG TTTCTATTAA GGTTGGTTCT 
ACTGCTGTTC AGGTAACCTC AGCAGAAAGA ACAAAAGTAT TGGGGCAATC CGTCTTTCTA 
AATGATATCT ACTATGCTTC TGAAATCGAA GAGATCTGCC TAGTTGATGA GAACCAGTTC 
ACTTTAACCA TTGCAAACCA AGGCACACCG CTGACCTTTA TGCACCAAGA GTGTGAAGCC 
ATTGTTCAGT CTATCATTCA TATCAGGACC CGATGGGAAC TGTCACAGCC TGACTCCATC 
CCTCAGCATA CCAAGATTCG ACCAAAAGAT GTCCCTGGAA CACTGCTTAA TATCGCATTA 
CTGAATTTGG GCAGTTCAGA CCCTAGTTTA CGGTCTGCTG CCTATAACCT CCTGTGTGCC 
TTAACTTGTA CCTTTAATTT AAAGATCGAA GGTCAGTTAC TAGAGACATC AGGGTTATGC 
ATCCCTGCCA ACAACACCCT CTTCATTGTC TCTATCAGTA AAACGCTTGC AGCCAACGAG 
CCACACCTCA CCTTAGAGTT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT 
GAATTGAAAC ACCTTTGTTT GGAATATATG ACCCCATGGC TGTCAAACCT AGTCCGTTTT 
TGTAAGCATA ACGATGACGC CAAACGACAA AGGGTTACTG CCATCCTTGA TAAACTCATA 
ACAATGACCA TAAATGAAAA GCAGATGTAC CCTTCTATTC AAGCAAAAAT ATGGGGGAGC 
CTTGGGCAGA TCACAGACCT GCTAGATGTT GTGCTTGACA GTTTCATCAA AACCAGTGCT 
ACCGGAGGGT TGGGGTCTAT CAAAGCTGAG GTGATGGCAG ACACAGCTGT GGCTTTAGCT 
TCTGGAAATG TGAAACTGGT GTCAAGTAAG GTTATTGGAA GGATGTGTAA AATAATTGAC 
AAGACTTGCT TATCTCCAAC TCCAACTTTA GAACAGCATC TTATGTGGGA CGACATTGCC 
ATTTTAGCCC GCTATATGCT GATGCTGTCC TTCAACAACT CCCTGGATGT GGCCGCTCAT 
CTCCCCTATC TCTTCCATGT TGTCACCTTC TTAGTAGCCA CAGGTCCTTT GTCCCTTCGC 
GCTTCCACCC ACGGATTGGT CATTAATATC ATTCACTCTC TGTGCACTTG CTCACAGCTT 
CATTTTAGTG AAGAGACCAA GCAAGTTTTG AGGCTCAGTC TGACAGAGTT CTCGTTACCC 
AAATTTTACT TACTGTTTGG CATCAGCAAA GTCAAGTCAG CTGCTGTCAT TGCCTTCCGT 
TCCAGCTACC GGGACCGGTC GTTCTCTCCT GGCTCCTATG AAAGGGAGAC TTTTGCTTTG 
ACGTCCCTGG AAACAGTCAC AGAAGCTTTG TTGGAGATCA TGGAGGCATG TATGAGAGAT 
ATTCCAACAT GCAAGTGGCT GGATCAGTGG ACGGAACTGG CTCAAAGATT TGCATTTCAG 
TACAACCCAT CCCTGCAGCC AAGAGCTCTT GTGGTGTTTG GCTGTATTAG CAAACGTGTG 
TCTCACGGGC AGATAAAGCA GATTATCCGA ATCCTTAGCA AGGCACTTGA AAGTTGTTTA 
AAGGGACCTG ACACTTACAA CAGTCAAGTT CTGATAGAAG CCACAGTGAT TGCACTGACC 
AAACTACAGC CACTTCTTAA TAAGGACTCA CCACTGCACA AAGCCCTCTT CTGGGTTGCT 
GTGGCTGTGC TGCAGCTAGA TGAAGTCAAC TTGTACTCAG CTGGCACTGC ACTTCTGGAA 
CAGAATCTGC ATACCTTGGA TAGTCTCCGG ATATTCAATG ACAAGAGTCC GGAAGAGGTA 
TTTATGGCAA TCCGGAATCC TCTGGAATGG CACTGCAAGC AAATGGATCA CTTTGTTGGA 
CTCAATTTCA ACTCTAATTT TAACTTTGCA CTAGTTGGAC ACCTCTTAAA AGGGTACAGA 
CATCCTTCAC CTGCCATTGT TGCAAGAACA GTCAGAATTT TGCATACACT ACTAACTCTT 
GTTAACAAAC ATAGAAATTG TGACAAATTT GAGGTGAATA CACAGAGTGT GGCTTACTTA 
GCAGCTCTAC TCACAGTGTC TGAAGAGGTT CGAAGTCGCT GTAGCCTCAA GCATAGGAAG 
TCCCTTCTTC TTACTGATAT CTCAATGGAA AATGTTCCTA TGGATACATA TCCCATTCAT 
CACGGTGATC CCAGCTCTAG GACACTAAAG GAGACTCAGC CATGGTCCTC TCCCAGAGGT 
TCAGAAGGAT ACCTTGCAGC CACCTACCCA GCTGTAGGCC AAACTAGTCC CCGAGCCAGG 
AAATCCATGA GCTTGGACAT GGGACAGCCT TCTCAGGCCA ACACAAAAAA ATTGCTTGGA 
ACAAGGAAAA GTTTTGATCA CTTGATATCA GACACAAAGG CTCCTAAAAG ACAAGAAATG 
GAATCAGGGA TTACAACACC CCCAAAAATG AGAAGAGTGG CAGAGACCGA CTATGAAATG 
GAAACCCAAA GGATTTCCTC CTCGCAGCAG CACCCACATT TACGGAAAGT CTCAGTGTCT 
GAATCTAATG TTCTATTGGA CGAAGAAGTA CTTACTGACC CAAAGATCCA AGCACTGCTT 
CTTACTGTTC TGGCTACACT GGTAAAATAT ACCACAGATG AATTTGATCA GCGAATTCTT 
TATGAATATC TAGCAGAGGC CAGTGTTGTG TTTCCCAAAG TGTTTCCTCT TGTGCACAAT 
TTGTTGGACT CTAAGATCAA TACCCTCTTG TCTTTGTGCC AAGATCCAAA TTTGTTAAAT 
CCGATCCATG GAATCGTGCA GAGTGTGGTG TACCATGAGG AATCCCCTCC ACAGTACCAA 
ACATCATACC TGCAAAGTTT CGGTTTTAAT GGCTTATGGC GGTTTGCAGG ACCCTTTTCA 
AAGCAAACAC AAATCCCAGA CTATGCTGAG CTCATTGTTA AATTTCTTGA TGCCTTGATT 
GACACGTACT TGCCTGGAAT TGATGAAGAA ACCAGTGAGG AGTCCCTCCT GACACCCACA 
TCTCCTTACC CTCCTGCACT GCAGAGCCAG CTTAGTATCA CTGCCAACCT TAACCTCTCT 
AATTCTATGA CCTCACTTGC AACTTCCCAG CATTCCCCAG GAATCGACAA GGAGAACGTT 
GAACTCTCCC CCACCACTGG CCACTGTAAC AGTGGACGGA CTCGCCATGG ATCCGCAAGC 
CAAGTGCAGA AGCAAAGGAG TGCTGGCAGT TTCAAACGTA ATAGCATTAA GAAGATCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036741.1
CDS	135..8597
Translation	MAAHRPVEWVQAVVSRFDEQLPIKTGQQNTHTKVSTEHNKECLINISKYKFSLVISGLTTILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTCREGNQHAAELRNSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLKETAFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQIPQTDMAECAGKLFDLVDGFAESTKRKAAVWPLQIILLILCPEIIQDISRDVVDENNTNKKLFLDSLRKALAGHGGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQPADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSAWDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHPALRMAPSLTFKEKVTSLKFKEKPTDLEARSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSHMPEVAQEAMEALLVLHQLDSIDLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKFLLKNKQADRSSCHSLYLYGVGCDLPASGNVTQMSVDHEESLRTCAPGASLRKGRGNSSMDSTAGCSGTPPICRQAQTKLEVALYMFLWSPDTEVVLVAMSCFRHLCEEADIRCGVDEVSVHNFLPNYNTFMEFASVSNMLSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTHAKWEQATKLILNYPKAKMEDGQAAESLHKTIVKRRMSHVSGGGSIDLSDTDSLQEWINMTGFLCALGGVCLQQRSSSGLATYSPPMGPVSERKGSMISVMSSEGNVDSPVSRFMDRLLSLMVCNHEKVGLQIRTNVKDLVGLELSPALYPMLFNKLKSAISKFFDSQGQVLLTDSNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRVLGNMVHAIQIKTKLCQLVEVMMARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDENAQTGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRIIITSSDWQHVSFEVDPTRLEPSESLEENQRNLLQMTEKFFHAIISSSSEFPSQLRSVCHCLYQVVSQRFPQNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIERGLKLMSKVLQSIANHVLFTKEEHMRPFNDFVKSNFDLARRFFLDIASDCPTSDAVNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLSIFYQAGTSKAGNPIFYYVARRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVVDLTHTGPSNRFKTDFLSKWFVVFPGFAYDNVSAVYIYNCNSWVREYTKYHERLLTGLKGSKRLIFIDCPGKLAEHIEHEQQKLPAATLALEEDLKVFHNALKLAHKDTKVSIKVGSTAVQVTSAERTKVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTFLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKALESCLKGPDTYNSQVLIEATVIALTKLQPLLNKDSPLHKALFWVAVAVLQLDEVNLYSAGTALLEQNLHTLDSLRIFNDKSPEEVFMAIRNPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGYRHPSPAIVARTVRILHTLLTLVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDISMENVPMDTYPIHHGDPSSRTLKETQPWSSPRGSEGYLAATYPAVGQTSPRARKSMSLDMGQPSQANTKKLLGTRKSFDHLISDTKAPKRQEMESGITTPPKMRRVAETDYEMETQRISSSQQHPHLRKVSVSESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYEYLAEASVVFPKVFPLVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHEESPPQYQTSYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEETSEESLLTPTSPYPPALQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCNSGRTRHGSASQVQKQRSAGSFKRNSIKKIV

Target ORF information:

RefSeq Version	NM_012609.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofibromin 1 (Nf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012609.1

ORF Insert Sequence:

ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGCACGGA GCACAACAAG 
GAGTGTCTGA TCAACATTTC CAAGTACAAG TTTTCTTTGG TCATCAGTGG ACTCACCACC 
ATTCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT 
CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC 
ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TACCAGAAAT CTGCCATTTT 
CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCTTCTGGG 
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGAAT TTCTACAAGG 
TTACAGGAGT TGACTGTCTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA 
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA 
TTTAAAGCCC TGAAGAAGGT GGCACAATTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT 
TGGAACTGGG TGGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA 
GATATGGCTG AGTGTGCAGG AAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC 
AAACGTAAAG CAGCAGTTTG GCCACTACAG ATCATTCTCC TCATTCTGTG TCCAGAAATA 
ATCCAGGACA TATCCAGGGA TGTGGTCGAT GAGAACAACA CAAACAAGAA ACTGTTTCTG 
GACAGTTTAC GGAAAGCACT TGCTGGCCAT GGCGGAAGCA GACAGCTGAC TGAAAGTGCT 
GCCATCGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGACAACTCT 
GTCATTTTCC TACTGGTTCA GTCCATGGTG GTTGATCTGA AGAACCTGCT TTTTAATCCA 
AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT 
GTCTCTTGCT TTCGGATCAG CCCTCACAAC AACCAACACT TTAAGATCTG TCTGGCTCAG 
AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TCCACCGCAT TATCACCAAT 
TCTGCATGGG ATTGGTGGCC TAAGATTGAC GCTGTATATT GTCACTCAGT TGAACTTCGA 
AATATGTTTG GTGAAACCCT TCATAAAGCA GTGCAAGGCT GTGGAGCACA CCCAGCATTA 
CGGATGGCAC CAAGTCTTAC CTTTAAGGAA AAGGTAACAA GCCTTAAGTT TAAAGAAAAG 
CCTACGGACC TAGAGGCAAG GAGCTACAAG TATCTTCTTT TGTCTATGGT GAAACTGATT 
CATGCAGACC CAAAGCTTTT GCTTTGTAAT CCAAGAAAAC AGGGCCCTGA AACTCAAGGT 
AGTACAGCAG AACTGATTAC AGGGCTCGTA CAGCTGGTTC CTCAGTCACA CATGCCAGAG 
GTCGCTCAGG AGGCTATGGA GGCTTTGTTG GTTCTTCATC AGTTAGACAG CATTGATCTG 
TGGAATCCTG ATGCTCCTGT AGAAACGTTT TGGGAAATTA GTTCACAAAT GCTTTTTTAC 
ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTA 
CGGGAAATTC TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT 
TCCTGTCATT CTCTCTACCT TTATGGAGTA GGATGTGACC TGCCTGCTAG TGGAAATGTC 
ACTCAGATGT CAGTTGATCA TGAGGAGTCA CTACGTACCT GTGCTCCTGG AGCCTCTCTG 
CGGAAAGGAA GAGGGAATTC CTCCATGGAT AGCACAGCAG GGTGCAGTGG AACCCCACCT 
ATATGCCGAC AAGCCCAGAC CAAGCTAGAA GTGGCCTTGT ACATGTTTCT GTGGAGTCCT 
GACACGGAAG TTGTTCTGGT TGCTATGTCC TGTTTCCGCC ACCTCTGTGA AGAAGCAGAC 
ATCCGATGTG GTGTGGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA CAACACATTC 
ATGGAATTTG CCTCAGTCAG CAATATGTTG TCAACAGGAA GAGCGGCACT TCAGAAAAGA 
GTGATGGCCC TGCTAAGGCG CATTGAGCAC CCTACTGCAG GAAACACTGA GGCCTGGGAA 
GATACACATG CAAAGTGGGA ACAGGCTACA AAACTAATCC TTAACTACCC AAAGGCCAAA 
ATGGAAGATG GCCAGGCTGC AGAAAGTCTT CATAAGACCA TTGTTAAGAG ACGGATGTCC 
CATGTCAGTG GAGGAGGATC CATAGACTTG TCTGACACAG ACTCCCTGCA GGAATGGATC 
AACATGACTG GCTTCCTCTG TGCCCTTGGT GGGGTGTGCC TGCAACAGAG AAGCAGCTCT 
GGCCTAGCAA CATACAGCCC ACCTATGGGC CCTGTCAGTG AACGCAAAGG GTCTATGATT 
TCTGTAATGT CTTCTGAAGG GAATGTTGAT TCACCTGTCA GCAGATTTAT GGACCGGCTT 
CTGTCCTTGA TGGTGTGTAA CCATGAGAAA GTGGGGCTTC AGATACGGAC CAATGTTAAG 
GACCTGGTGG GTCTGGAGCT GAGTCCTGCT CTGTATCCAA TGCTGTTCAA CAAACTGAAG 
AGTGCCATCA GCAAGTTTTT TGACTCTCAA GGACAGGTAT TATTAACTGA CAGCAATACT 
CAGTTTGTAG AGCAAACCAT AGCTATAATG AAGAACCTAC TGGATAATCA TACTGAAGGC 
AGCTCTGAGC ATCTGGGACA AGCTAGCATT GAGACAATGA TGCTAAATCT GGTCAGATAT 
GTTCGTGTTC TTGGGAATAT GGTTCATGCA ATTCAAATAA AAACGAAATT GTGTCAGTTA 
GTTGAAGTCA TGATGGCAAG AAGAGACGAC CTCTCGTTTT GTCAAGAGAT GAAATTTCGG 
AATAAGATGG TGGAGTACCT AACAGACTGG GTTATGGGCA CTTCAAACCA AGCCGCAGAT 
GATGATGTAA AGTGTCTCAC AAGAGATCTA GACCAAGCAA GCATGGAGGC AGTAGTCTCG 
CTCCTCGCTG GTCTTCCACT GCAGCCTGAA GAAGGAGACG GAGTGGAGCT GATGGAGGCC 
AAGTCACAGC TATTCCTTAA GTACTTCACA TTATTTATGA ACCTTTTGAA TGACTGTAGT 
GAAGTTGAAG ATGAAAATGC ACAAACAGGT GGCAGGAAAC GTGGCATGTC TCGGAGGCTG 
GCATCCCTGA GGCACTGTAC AGTGCTTGCA ATGTCAAACT TACTCAATGC TAATGTGGAC 
AGTGGACTCA TGCACTCCAT AGGCTTAGGT TATCATAAAG ATCTTCAGAC AAGAGCTACA 
TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGAACAG AATTTGATAC ACTTGCTGAA 
ACCGTGTTGG CAGATCGGTT CGAGAGACTT GTGGAACTGG TCACAATGAT GGGAGACCAG 
GGAGAGCTCC CTATAGCTAT GGCTCTGGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAG 
CTGGCTCGAG TTCTGGTCAC GCTGTTTGAT TCTCGGCATT TGCTCTACCA GCTGCTCTGG 
AACATGTTTT CTAAGGAGGT AGAGTTGGCA GACTCCATGC AGACTCTCTT TCGAGGCAAC 
AGCTTGGCCA GTAAGATAAT GACGTTCTGC TTCAAGGTGT ACGGTGCTAC TTACCTACAA 
AAGCTCTTGG ACCCCTTGTT ACGAATCATT ATCACATCCT CCGATTGGCA GCATGTTAGC 
TTTGAAGTGG ATCCTACCAG GTTAGAGCCC TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC 
CTCCTTCAGA TGACAGAGAA GTTCTTCCAT GCCATCATCA GTTCTTCCTC AGAGTTCCCC 
TCGCAGCTTC GAAGTGTCTG CCATTGTTTG TACCAGGTGG TTAGCCAGCG CTTCCCTCAG 
AACAGCATCG GTGCCGTCGG AAGTGCCATG TTCCTGAGGT TCATCAACCC TGCCATTGTC 
TCACCGTATG AAGCAGGGAT TTTAGATAAA AAGCCACCAC CTAGAATTGA AAGGGGCTTG 
AAGTTAATGT CAAAGGTCCT TCAGAGCATT GCCAATCATG TACTGTTCAC AAAGGAAGAG 
CACATGAGGC CTTTTAATGA TTTTGTGAAA AGCAACTTCG ACTTGGCACG GAGGTTTTTC 
TTAGATATAG CATCAGATTG TCCCACAAGT GACGCAGTAA ACCATAGTCT TTCCTTCATC 
AGTGATGGCA ATGTGCTCGC TTTACATCGG CTGCTTTGGA ATAATCAGGA GAAAATTGGC 
CAGTATCTTT CCAGCAACAG GGATCATAAA GCTGTTGGAA GACGACCTTT CGATAAGATG 
GCCACACTTC TTGCATATCT GGGTCCTCCG GAGCACAAGC CTGTGGCAGA TACACACTGG 
TCCAGCCTTA ACCTTACCAG TTCAAAGTTT GAGGAATTTA TGACCAGGCA TCAGGTTCAT 
GAGAAGGAAG AGTTCAAGGC CTTGAAAACA TTAAGCATCT TCTACCAAGC TGGCACATCC 
AAAGCTGGGA ATCCTATTTT CTATTATGTT GCACGGAGGT TCAAAACTGG CCAAATCAAT 
GGGGATTTGC TGATATACCA TGTCTTGCTG ACTTTAAAGC CATATTATGC AAAGCCATAT 
GAAATTGTAG TGGACCTTAC CCATACTGGG CCTAGCAATC GCTTTAAAAC AGACTTCCTC 
TCTAAGTGGT TTGTTGTTTT TCCTGGCTTT GCTTATGACA ACGTGTCTGC AGTCTATATC 
TATAACTGTA ACTCCTGGGT CAGGGAGTAC ACCAAATACC ATGAGCGGCT GCTGACTGGC 
CTCAAGGGCA GCAAAAGGCT CATTTTCATC GACTGTCCTG GGAAGCTGGC TGAGCACATA 
GAGCACGAGC AACAGAAGCT CCCTGCTGCT ACCTTGGCAC TGGAAGAGGA TCTAAAGGTT 
TTCCACAATG CGCTCAAGCT AGCTCACAAG GACACCAAAG TTTCTATTAA GGTTGGTTCT 
ACTGCTGTTC AGGTAACCTC AGCAGAAAGA ACAAAAGTAT TGGGGCAATC CGTCTTTCTA 
AATGATATCT ACTATGCTTC TGAAATCGAA GAGATCTGCC TAGTTGATGA GAACCAGTTC 
ACTTTAACCA TTGCAAACCA AGGCACACCG CTGACCTTTA TGCACCAAGA GTGTGAAGCC 
ATTGTTCAGT CTATCATTCA TATCAGGACC CGATGGGAAC TGTCACAGCC TGACTCCATC 
CCTCAGCATA CCAAGATTCG ACCAAAAGAT GTCCCTGGAA CACTGCTTAA TATCGCATTA 
CTGAATTTGG GCAGTTCAGA CCCTAGTTTA CGGTCTGCTG CCTATAACCT CCTGTGTGCC 
TTAACTTGTA CCTTTAATTT AAAGATCGAA GGTCAGTTAC TAGAGACATC AGGGTTATGC 
ATCCCTGCCA ACAACACCCT CTTCATTGTC TCTATCAGTA AAACGCTTGC AGCCAACGAG 
CCACACCTCA CCTTAGAGTT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT 
GAATTGAAAC ACCTTTGTTT GGAATATATG ACCCCATGGC TGTCAAACCT AGTCCGTTTT 
TGTAAGCATA ACGATGACGC CAAACGACAA AGGGTTACTG CCATCCTTGA TAAACTCATA 
ACAATGACCA TAAATGAAAA GCAGATGTAC CCTTCTATTC AAGCAAAAAT ATGGGGGAGC 
CTTGGGCAGA TCACAGACCT GCTAGATGTT GTGCTTGACA GTTTCATCAA AACCAGTGCT 
ACCGGAGGGT TGGGGTCTAT CAAAGCTGAG GTGATGGCAG ACACAGCTGT GGCTTTAGCT 
TCTGGAAATG TGAAACTGGT GTCAAGTAAG GTTATTGGAA GGATGTGTAA AATAATTGAC 
AAGACTTGCT TATCTCCAAC TCCAACTTTA GAACAGCATC TTATGTGGGA CGACATTGCC 
ATTTTAGCCC GCTATATGCT GATGCTGTCC TTCAACAACT CCCTGGATGT GGCCGCTCAT 
CTCCCCTATC TCTTCCATGT TGTCACCTTC TTAGTAGCCA CAGGTCCTTT GTCCCTTCGC 
GCTTCCACCC ACGGATTGGT CATTAATATC ATTCACTCTC TGTGCACTTG CTCACAGCTT 
CATTTTAGTG AAGAGACCAA GCAAGTTTTG AGGCTCAGTC TGACAGAGTT CTCGTTACCC 
AAATTTTACT TACTGTTTGG CATCAGCAAA GTCAAGTCAG CTGCTGTCAT TGCCTTCCGT 
TCCAGCTACC GGGACCGGTC GTTCTCTCCT GGCTCCTATG AAAGGGAGAC TTTTGCTTTG 
ACGTCCCTGG AAACAGTCAC AGAAGCTTTG TTGGAGATCA TGGAGGCATG TATGAGAGAT 
ATTCCAACAT GCAAGTGGCT GGATCAGTGG ACGGAACTGG CTCAAAGATT TGCATTTCAG 
TACAACCCAT CCCTGCAGCC AAGAGCTCTT GTGGTGTTTG GCTGTATTAG CAAACGTGTG 
TCTCACGGGC AGATAAAGCA GATTATCCGA ATCCTTAGCA AGGCACTTGA AAGTTGTTTA 
AAGGGACCTG ACACTTACAA CAGTCAAGTT CTGATAGAAG CCACAGTGAT TGCACTGACC 
AAACTACAGC CACTTCTTAA TAAGGACTCA CCACTGCACA AAGCCCTCTT CTGGGTTGCT 
GTGGCTGTGC TGCAGCTAGA TGAAGTCAAC TTGTACTCAG CTGGCACTGC ACTTCTGGAA 
CAGAATCTGC ATACCTTGGA TAGTCTCCGG ATATTCAATG ACAAGAGTCC GGAAGAGGTA 
TTTATGGCAA TCCGGAATCC TCTGGAATGG CACTGCAAGC AAATGGATCA CTTTGTTGGA 
CTCAATTTCA ACTCTAATTT TAACTTTGCA CTAGTTGGAC ACCTCTTAAA AGGGTACAGA 
CATCCTTCAC CTGCCATTGT TGCAAGAACA GTCAGAATTT TGCATACACT ACTAACTCTT 
GTTAACAAAC ATAGAAATTG TGACAAATTT GAGGTGAATA CACAGAGTGT GGCTTACTTA 
GCAGCTCTAC TCACAGTGTC TGAAGAGGTT CGAAGTCGCT GTAGCCTCAA GCATAGGAAG 
TCCCTTCTTC TTACTGATAT CTCAATGGAA AATGTTCCTA TGGATACATA TCCCATTCAT 
CACGGTGATC CCAGCTCTAG GACACTAAAG GAGACTCAGC CATGGTCCTC TCCCAGAGGT 
TCAGAAGGAT ACCTTGCAGC CACCTACCCA GCTGTAGGCC AAACTAGTCC CCGAGCCAGG 
AAATCCATGA GCTTGGACAT GGGACAGCCT TCTCAGGCCA ACACAAAAAA ATTGCTTGGA 
ACAAGGAAAA GTTTTGATCA CTTGATATCA GACACAAAGG CTCCTAAAAG ACAAGAAATG 
GAATCAGGGA TTACAACACC CCCAAAAATG AGAAGAGTGG CAGAGACCGA CTATGAAATG 
GAAACCCAAA GGATTTCCTC CTCGCAGCAG CACCCACATT TACGGAAAGT CTCAGTGTCT 
GAATCTAATG TTCTATTGGA CGAAGAAGTA CTTACTGACC CAAAGATCCA AGCACTGCTT 
CTTACTGTTC TGGCTACACT GGTAAAATAT ACCACAGATG AATTTGATCA GCGAATTCTT 
TATGAATATC TAGCAGAGGC CAGTGTTGTG TTTCCCAAAG TGTTTCCTCT TGTGCACAAT 
TTGTTGGACT CTAAGATCAA TACCCTCTTG TCTTTGTGCC AAGATCCAAA TTTGTTAAAT 
CCGATCCATG GAATCGTGCA GAGTGTGGTG TACCATGAGG AATCCCCTCC ACAGTACCAA 
ACATCATACC TGCAAAGTTT CGGTTTTAAT GGCTTATGGC GGTTTGCAGG ACCCTTTTCA 
AAGCAAACAC AAATCCCAGA CTATGCTGAG CTCATTGTTA AATTTCTTGA TGCCTTGATT 
GACACGTACT TGCCTGGAAT TGATGAAGAA ACCAGTGAGG AGTCCCTCCT GACACCCACA 
TCTCCTTACC CTCCTGCACT GCAGAGCCAG CTTAGTATCA CTGCCAACCT TAACCTCTCT 
AATTCTATGA CCTCACTTGC AACTTCCCAG CATTCCCCAG GAATCGACAA GGAGAACGTT 
GAACTCTCCC CCACCACTGG CCACTGTAAC AGTGGACGGA CTCGCCATGG ATCCGCAAGC 
CAAGTGCAGA AGCAAAGGAG TGCTGGCAGT TTCAAACGTA ATAGCATTAA GAAGATCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neurofibromin Regulates Seizure Attacks in the Rat Pilocarpine-Induced Model of Epilepsy.
Molecular neurobiology53(9)6069-6077(2016 Nov)
Ren M,Li K,Wang D,Guo J,Li J,Yang G,Long X,Shen W,Hu R,Wang X,Zeng K

Clinicopathologic implications of NF1 gene alterations in diffuse gliomas.
Human pathology46(9)1323-30(2015 Sep)
Vizca?no MA,Shah S,Eberhart CG,Rodriguez FJ

Social learning and amygdala disruptions in Nf1 mice are rescued by blocking p21-activated kinase.
Nature neuroscience17(11)1583-90(2014 Nov)
Molosh AI,Johnson PL,Spence JP,Arendt D,Federici LM,Bernabe C,Janasik SP,Segu ZM,Khanna R,Goswami C,Zhu W,Park SJ,Li L,Mechref YS,Clapp DW,Shekhar A

Identification of genes important for cutaneous function revealed by a large scale reverse genetic screen in the mouse.
PLoS genetics10(10)e1004705(2014 Oct)
DiTommaso T,Jones LK,Cottle DL,,Gerdin AK,Vancollie VE,Watt FM,Ramirez-Solis R,Bradley A,Steel KP,Sundberg JP,White JK,Smyth IM

Neurofibromin is the major ras inactivator in dendritic spines.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(3)776-83(2014 Jan)
Oliveira AF,Yasuda R

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Nf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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