NF1 cDNA ORF clone, Canis lupus familiaris(dog)

The following NF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe290773 XM_022423324.1
Latest version!
Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe290774 XM_022423325.1
Latest version!
Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe290775 XM_022423326.1
Latest version!
Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe290776 XM_022423327.1
Latest version!
Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe290777 XM_022423328.1
Latest version!
Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe290778 XM_022423329.1
Latest version!
Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe38290 XM_014116492.1
Latest version!
Canis lupus familiaris neurofibromin 1 (NF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe290773
    Clone ID Related Accession (Same CDS sequence) XM_022423324.1
    Accession Version XM_022423324.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofibromin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
    GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA
    ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA
    AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC
    AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT
    TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT
    GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT
    GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT
    CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA
    AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG
    CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT
    GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC
    GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC
    TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG
    TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC
    AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC
    CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC
    TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT
    GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT
    GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC
    CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT
    AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG
    AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA
    ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT
    ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC
    ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG
    CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC
    AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCC
    GATAGAAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCAAGT
    GGAACTACTA GTCAGATGTC CATGGATCAT GAAGAATTAC TACGTTCTTG TACTCCTGGA
    GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCAGCAGG GTGCAGCGGT
    ACTCCGCCAA TATGCCGACA AGCTCAGACT AAACTAGAAG TGGCCCTGTA CATGTTTCTG
    TGGAGCCCTG ACACTGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGTCA CCTTTGTGAG
    GAAGCAGATA TCCGGTGTGG GGTGGACGAA GTGTCGGTGC ACAACTTCTT GCCCAACTAT
    AACACATTTA TGGAATTTGC CTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT
    CAGAAAAGAG TGATGGCACT ACTAAGGCGC ATTGAGCATC CCACTGCAGG AAATACCGAG
    GCTTGGGAAG ATACACATGC GAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTACCCA
    AAAGCTAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ACAAGACCAT TGTTAAGAGG
    CGAATGTCTC ATGTCAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTACAG
    GAATGGATCA ACATGACTGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA
    AGCAATTCTG GCTTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGTAAGGGT
    TCCATGATTT CAGTGATGTC TTCAGAGGGA AATGCAGATA CACCTGTCAG CAAATTTATG
    GATCGGCTGT TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGGACC
    AATGTTAAGG ATCTGGTGGG TCTAGAATTG AGTCCTGCTC TTTATCCAAT GCTATTTAAC
    AAATTGAAGA ATACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC
    ACCAATACTC AGTTTGTGGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT
    ACTGAAGGCA GCTCTGAACA CCTGGGGCAA GCTAGCATTG AAACAATGAT GTTAAATCTA
    GTCAGATATG TTCGTGTGCT TGGGAATATG GTCCATGCAA TTCAAATAAA AACGAAACTC
    TGTCAGTTAG TCGAGGTGAT GATGGCGAGG AGAGATGACC TCTCATTTTG TCAAGAGATG
    AAATTTAGGA ATAAGATGGT AGAATACCTG ACAGACTGGG TTATGGGAAC ATCCAACCAG
    GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGACTTGG ACCAGGCAAG CATGGAAGCA
    GTAGTTTCAC TCCTGGCTGG TCTTCCACTG CAGCCGGAGG AAGGAGATGG TGTGGAATTG
    ATGGAAGCCA AGTCACAGTT ATTTCTTAAG TATTTCACAT TGTTCATGAA CCTATTGAAT
    GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAACG TGGCATGTCT
    CGGAGGCTGG CATCACTGAG GCACTGTACA GTCCTTGCAA TGTCAAACTT ACTCAATGCT
    AATGTGGACA GTGGCCTCAT GCATTCCATA GGTTTAGGTT ATCACAAAGA TCTTCAGACA
    AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA
    CTTGCGGAAA CAGTGCTGGC CGACCGGTTT GAGAGGCTGG TGGAGTTGGT GACGATGATG
    GGAGACCAGG GAGAGCTCCC AATAGCCATG GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG
    TGGGATGAAC TAGCTCGAGT TTTGGTGACT CTGTTTGATT CTCGGCATTT ACTCTACCAA
    TTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC
    CGAGGAAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC
    TACCTACAAA AACTCTTGGA TCCGTTATTA CGAATTGTGA TCACATCCTC TGATTGGCAG
    CATGTTAGCT TTGAAGTGGA TCCTACCAGA TTAGAACAGT CAGAGAGCCT TGAGGAAAAC
    CAACGGAACC TTCTTCAGAT GACTGAAAAG TTCTTCCATG CCATCATCAG TTCCTCCTCA
    GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGCAAC TTGCCACTCC
    CTACTGAATA AAGCTACAGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT GGTTAGCCAG
    CGTTTCCCTC AGAACAGCAT CGGTGCAGTG GGAAGTGCCA TGTTCCTCAG ATTTATCAAT
    CCTGCCATTG TCTCACCGTA CGAAGCAGGG ATTTTAGATA AAAAGCCACC ACCTAGAATT
    GAAAGGGGCT TGAAGTTAAT GTCAAAGATA CTTCAGAGTA TTGCCAATCA TGTTCTTTTC
    ACAAAAGAAG AGCATATGCG GCCTTTTAAT GATTTTGTGA AGAGCAACTT TGATGCAGCA
    CGGAGGTTTT TCCTTGATAT AGCATCTGAT TGTCCTACAA GTGATACAGT AAATCATAGT
    CTTTCCTTCA TCAGTGATGG CAATGTGCTT GCTTTACATC GTCTACTCTG GAACAATCAA
    GAGAAAATTG GCCAGTATCT TTCCAGCAAC AGGGATCACA AAGCTGTTGG AAGACGACCT
    TTCGATAAGA TGGCAACACT TCTTGCATAC CTGGGTCCTC CAGAGCACAA ACCTGTGGCA
    GATACACACT GGTCCAGCCT TAACCTTACC AGTTCAAAGT TTGAGGAATT TATGACTAGG
    CATCAAGTAC ATGAAAAAGA AGAGTTCAAG GCTTTGAAAA CATTAAGTAT TTTCTACCAA
    GCAGGGACTT CCAAAGCTGG GAATCCTATA TTTTATTATG TTGCACGGAG GTTTAAAACT
    GGTCAAATCA ATGGCGATTT GCTGATATAC CATGTCTTGC TGACTTTAAA GCCATATTAT
    GCAAAGCCAT ATGAAATTGT AGTGGACCTT ACGCATACTG GGCCGAGCAA TCGCTTTAAA
    ACAGACTTTC TCTCCAAGTG GTTTGTTGTT TTTCCTGGCT TCGCTTATGA CAATGTCTCT
    GCAGTCTATA TCTATAACTG TAACTCCTGG GTCAGAGAGT ACACCAAGTA TCACGAGCGG
    CTGCTGACTG GCCTCAAAGG CAGCAAAAGG CTCATCTTCA TAGACTGTCC TGGGAAACTG
    GCTGAGCACA TAGAGCATGA ACAACAGAAA CTGCCTGCTG CCACACTGGC TTTAGAAGAG
    GACCTGAAGG TATTCCACAA TGCTCTCAAG CTAGCTCACA AAGACACCAA GGTTTCCATT
    AAAGTTGGTT CTACTGCTGT TCAAGTAACC TCAGCAGAGC GAACAAAAGT CCTGGGGCAA
    TCGGTCTTTC TAAATGATAT TTATTATGCT TCGGAAATTG AAGAGATCTG CCTGGTAGAT
    GAGAACCAGT TTACCTTAAC CATTGCAAAC CAGGGCACAC CACTCACCTT CATGCACCAG
    GAGTGTGAAG CCATTGTTCA GTCTATCATC CACATCCGGA CACGCTGGGA GCTGTCACAA
    CCTGACTCCA TCCCCCAACA CACCAAGATT CGGCCAAAAG ATGTCCCTGG GACGCTGCTC
    AATATTGCGT TACTTAATTT AGGCAGTTCA GACCCTAGTT TACGGTCAGC TGCCTATAAT
    CTTCTGTGTG CCTTAACTTG TACCTTCAAT TTAAAAATCG AGGGCCAATT ACTAGAGACA
    TCAGGTTTAT GCATCCCTGC CAACAACACC CTCTTTATTG TCTCTATTAG TAAGACACTG
    GCAGCCAATG AGCCACATCT GACCTTAGAA TTTTTGGAAG AGTGTATTTC TGGATTTAGC
    AAGTCTAGTA TTGAATTGAA ACACCTTTGT TTGGAATACA TGACTCCGTG GCTGTCAAAT
    CTAGTCCGTT TTTGTAAGCA CAATGATGAT GCCAAAAGAC AGAGAGTTAC TGCTATTCTT
    GATAAGCTGA TAACAATGAC CATAAATGAG AAACAGATGT ACCCATCTAT CCAGGCCAAA
    ATATGGGGGA GCCTTGGGCA GATTACAGAT CTCCTTGATG TTGTATTAGA CAGTTTCATC
    AAAACCAGTG CAACAGGTGG CTTGGGATCA ATAAAAGCTG AGGTGATGGC AGATACTGCT
    GTAGCTTTGG CTTCTGGAAA CGTGAAATTG GTTTCAAGCA AGGTTATTGG AAGGATGTGC
    AAAATAATTG ACAAGACATG TTTATCTCCA ACTCCTACCT TAGAGCAACA TCTTATGTGG
    GACGATATTG CTATTCTAGC ACGCTACATG CTGATGCTGT CCTTCAACAA TTCCCTTGAT
    GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT GTTGTTACTT TCTTAGTAGC TACAGGGCCA
    CTCTCCCTTA GAGCTTCCAC ACATGGATTG GTCATTAATA TCATTCACTC TCTGTGTACT
    TGTTCACAGC TTCATTTTAG TGAAGAGACA AAGCAAGTTT TGAGACTCAG TTTGACAGAG
    TTCTCATTAC CCAAGTTTTA CCTGCTGTTT GGCATTAGTA AAGTCAAGTC AGCTGCCGTC
    ATTGCCTTTC GCTCCAGTTA CCGGGACAGG TCATTCTCTC CTGGCTCCTA TGAAAGGGAG
    ACTTTTGCTT TGACATCCTT GGAAACAGTC ACAGAAGCTT TGTTGGAGAT TATGGAGGCA
    TGTATGAGAG ATATTCCAAC ATGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAAAGA
    TTTGCATTCC AATATAATCC ATCCCTGCAA CCAAGAGCTC TTGTTGTCTT TGGCTGTATT
    AGCAAACGAG TGTCTCATGG GCAGATAAAA CAGATTATCC GTATTCTTAG CAAGGCACTT
    GAGAGTTGCT TAAAAGGACC TGATACTTAC AACAGTCAAG TTCTGATAGA AGCTACAGTA
    ATAGCACTAA CCAAATTACA ACCACTTCTT AATAAGGACT CCCCTCTGCA CAAAGCCCTC
    TTTTGGGTGG CTGTGGCTGT GCTACAGCTC GATGAGGTCA ACCTGTATTC AGCAGGCAGT
    GCGCTTCTGG AACAAAACCT GCACACCTTA GACAGTCTGC GGGTATTCAA TGACAAGAGT
    CCAGAGGAAG TATTTATGGC AATCCGGAAT CCTCTGGAAT GGCACTGCAA ACAAATGGAT
    CATTTTGTTG GACTCAATTT CAACTCTAAC TTTAACTTCG CACTGGTTGG ACACCTCTTA
    AAAGGGTACA GGCATCCTTC ACCTGCCATT GTGGCAAGAA CAGTCAGAAT TTTGCATACA
    CTACTAACTC TGGTGAACAA ACATAGAAAC TGTGACAAAT TTGAGGTGAA CACACAGAGC
    GTGGCTTATT TAGCAGCCTT ACTCACAGTG TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA
    AAACATAGAA AATCTCTTCT TCTTACTGAT ATTTCAATGG AAAATGTTCC TATGGATACG
    TATCCCATCC ATCATGGCGA CCCTAGCTAT AGGACGCTAA AGGAGAACCA GCCGTGGTCA
    TCTCCCAAAG GTTCTGAAGG GTACCTTGCA GCCTCCTATC CAGCCGTGGG CCAGACCAGT
    CCCCGAGCCA GGAAATCCAT GAGTTTGGAC ATGGGGCAGC CTTCTCAGGC CAATACGAAG
    AAGTTGCTGG GAACAAGGAA AAGTTTTGAT CACTTGATAT CAGACACAAA GGCTCCTAAA
    AGGCAAGAAA TGGAATCAGG GATCACAACA CCCCCCAAAA TGAGGAGAGT AGCAGAAACT
    GATTATGAAA TGGAAACTCA GAGGATTTCC TCATCACAGC AACACCCACA TTTACGCAAA
    GTTTCAGTGT CTGAATCAAA TGTTCTCTTG GATGAAGAAG TACTTACTGA TCCGAAGATC
    CAAGCACTTC TTCTTACTGT TCTGGCTACA CTGGTAAAAT ACACCACAGA TGAGTTTGAT
    CAGCGAATTC TTTATGAATA CTTAGCAGAA GCCAGTGTTG TTTTCCCCAA GGTTTTTCCT
    GTTGTGCACA ATTTGTTGGA CTCTAAGATC AACACCTTGT TGTCATTGTG CCAAGATCCA
    AATTTGTTAA ATCCAATCCA TGGAATCGTG CAGAGTGTGG TATACCATGA AGAATCCCCA
    CCTCAGTACC AAACATCTTA TCTGCAAAGT TTTGGTTTTA ATGGCTTATG GCGGTTTGCA
    GGACCCTTTT CAAAGCAAAC ACAAATCCCA GACTATGCTG AGCTTATTGT TAAGTTTCTT
    GATGCCTTGA TTGACACGTA TTTGCCTGGA ATTGATGAAG AAACCAGTGA GGAGTCCCTC
    CTGACACCCA CTTCTCCTTA TCCTCCTGCA GTGCAGAGCC AGCTTAGTAT TACTGCTAAT
    CTTAACCTTT CTAATTCCAT GACCTCACTT GCAACTTCCC AGCATTCCCC AGGAATCGAC
    AAGGAGAACG TTGAACTCTC CCCAACCACT GGGCACTGTA ACAGTGGACG GACTCGCCAT
    GGATCCGCAA GCCAAGTGCA GAAGCAAAGA AGCGCTGGCA GTTTCAAACG TAATAGCATT
    AAGAAGATCG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279032.1
    CDS148..8502
    Translation

    Target ORF information:

    RefSeq Version XM_022423324.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423324.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
    GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA
    ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA
    AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC
    AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT
    TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT
    GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT
    GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT
    CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA
    AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG
    CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT
    GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC
    GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC
    TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG
    TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC
    AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC
    CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC
    TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT
    GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT
    GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC
    CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT
    AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG
    AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA
    ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT
    ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC
    ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG
    CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC
    AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCC
    GATAGAAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCAAGT
    GGAACTACTA GTCAGATGTC CATGGATCAT GAAGAATTAC TACGTTCTTG TACTCCTGGA
    GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCAGCAGG GTGCAGCGGT
    ACTCCGCCAA TATGCCGACA AGCTCAGACT AAACTAGAAG TGGCCCTGTA CATGTTTCTG
    TGGAGCCCTG ACACTGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGTCA CCTTTGTGAG
    GAAGCAGATA TCCGGTGTGG GGTGGACGAA GTGTCGGTGC ACAACTTCTT GCCCAACTAT
    AACACATTTA TGGAATTTGC CTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT
    CAGAAAAGAG TGATGGCACT ACTAAGGCGC ATTGAGCATC CCACTGCAGG AAATACCGAG
    GCTTGGGAAG ATACACATGC GAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTACCCA
    AAAGCTAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ACAAGACCAT TGTTAAGAGG
    CGAATGTCTC ATGTCAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTACAG
    GAATGGATCA ACATGACTGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA
    AGCAATTCTG GCTTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGTAAGGGT
    TCCATGATTT CAGTGATGTC TTCAGAGGGA AATGCAGATA CACCTGTCAG CAAATTTATG
    GATCGGCTGT TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGGACC
    AATGTTAAGG ATCTGGTGGG TCTAGAATTG AGTCCTGCTC TTTATCCAAT GCTATTTAAC
    AAATTGAAGA ATACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC
    ACCAATACTC AGTTTGTGGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT
    ACTGAAGGCA GCTCTGAACA CCTGGGGCAA GCTAGCATTG AAACAATGAT GTTAAATCTA
    GTCAGATATG TTCGTGTGCT TGGGAATATG GTCCATGCAA TTCAAATAAA AACGAAACTC
    TGTCAGTTAG TCGAGGTGAT GATGGCGAGG AGAGATGACC TCTCATTTTG TCAAGAGATG
    AAATTTAGGA ATAAGATGGT AGAATACCTG ACAGACTGGG TTATGGGAAC ATCCAACCAG
    GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGACTTGG ACCAGGCAAG CATGGAAGCA
    GTAGTTTCAC TCCTGGCTGG TCTTCCACTG CAGCCGGAGG AAGGAGATGG TGTGGAATTG
    ATGGAAGCCA AGTCACAGTT ATTTCTTAAG TATTTCACAT TGTTCATGAA CCTATTGAAT
    GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAACG TGGCATGTCT
    CGGAGGCTGG CATCACTGAG GCACTGTACA GTCCTTGCAA TGTCAAACTT ACTCAATGCT
    AATGTGGACA GTGGCCTCAT GCATTCCATA GGTTTAGGTT ATCACAAAGA TCTTCAGACA
    AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA
    CTTGCGGAAA CAGTGCTGGC CGACCGGTTT GAGAGGCTGG TGGAGTTGGT GACGATGATG
    GGAGACCAGG GAGAGCTCCC AATAGCCATG GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG
    TGGGATGAAC TAGCTCGAGT TTTGGTGACT CTGTTTGATT CTCGGCATTT ACTCTACCAA
    TTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC
    CGAGGAAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC
    TACCTACAAA AACTCTTGGA TCCGTTATTA CGAATTGTGA TCACATCCTC TGATTGGCAG
    CATGTTAGCT TTGAAGTGGA TCCTACCAGA TTAGAACAGT CAGAGAGCCT TGAGGAAAAC
    CAACGGAACC TTCTTCAGAT GACTGAAAAG TTCTTCCATG CCATCATCAG TTCCTCCTCA
    GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGCAAC TTGCCACTCC
    CTACTGAATA AAGCTACAGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT GGTTAGCCAG
    CGTTTCCCTC AGAACAGCAT CGGTGCAGTG GGAAGTGCCA TGTTCCTCAG ATTTATCAAT
    CCTGCCATTG TCTCACCGTA CGAAGCAGGG ATTTTAGATA AAAAGCCACC ACCTAGAATT
    GAAAGGGGCT TGAAGTTAAT GTCAAAGATA CTTCAGAGTA TTGCCAATCA TGTTCTTTTC
    ACAAAAGAAG AGCATATGCG GCCTTTTAAT GATTTTGTGA AGAGCAACTT TGATGCAGCA
    CGGAGGTTTT TCCTTGATAT AGCATCTGAT TGTCCTACAA GTGATACAGT AAATCATAGT
    CTTTCCTTCA TCAGTGATGG CAATGTGCTT GCTTTACATC GTCTACTCTG GAACAATCAA
    GAGAAAATTG GCCAGTATCT TTCCAGCAAC AGGGATCACA AAGCTGTTGG AAGACGACCT
    TTCGATAAGA TGGCAACACT TCTTGCATAC CTGGGTCCTC CAGAGCACAA ACCTGTGGCA
    GATACACACT GGTCCAGCCT TAACCTTACC AGTTCAAAGT TTGAGGAATT TATGACTAGG
    CATCAAGTAC ATGAAAAAGA AGAGTTCAAG GCTTTGAAAA CATTAAGTAT TTTCTACCAA
    GCAGGGACTT CCAAAGCTGG GAATCCTATA TTTTATTATG TTGCACGGAG GTTTAAAACT
    GGTCAAATCA ATGGCGATTT GCTGATATAC CATGTCTTGC TGACTTTAAA GCCATATTAT
    GCAAAGCCAT ATGAAATTGT AGTGGACCTT ACGCATACTG GGCCGAGCAA TCGCTTTAAA
    ACAGACTTTC TCTCCAAGTG GTTTGTTGTT TTTCCTGGCT TCGCTTATGA CAATGTCTCT
    GCAGTCTATA TCTATAACTG TAACTCCTGG GTCAGAGAGT ACACCAAGTA TCACGAGCGG
    CTGCTGACTG GCCTCAAAGG CAGCAAAAGG CTCATCTTCA TAGACTGTCC TGGGAAACTG
    GCTGAGCACA TAGAGCATGA ACAACAGAAA CTGCCTGCTG CCACACTGGC TTTAGAAGAG
    GACCTGAAGG TATTCCACAA TGCTCTCAAG CTAGCTCACA AAGACACCAA GGTTTCCATT
    AAAGTTGGTT CTACTGCTGT TCAAGTAACC TCAGCAGAGC GAACAAAAGT CCTGGGGCAA
    TCGGTCTTTC TAAATGATAT TTATTATGCT TCGGAAATTG AAGAGATCTG CCTGGTAGAT
    GAGAACCAGT TTACCTTAAC CATTGCAAAC CAGGGCACAC CACTCACCTT CATGCACCAG
    GAGTGTGAAG CCATTGTTCA GTCTATCATC CACATCCGGA CACGCTGGGA GCTGTCACAA
    CCTGACTCCA TCCCCCAACA CACCAAGATT CGGCCAAAAG ATGTCCCTGG GACGCTGCTC
    AATATTGCGT TACTTAATTT AGGCAGTTCA GACCCTAGTT TACGGTCAGC TGCCTATAAT
    CTTCTGTGTG CCTTAACTTG TACCTTCAAT TTAAAAATCG AGGGCCAATT ACTAGAGACA
    TCAGGTTTAT GCATCCCTGC CAACAACACC CTCTTTATTG TCTCTATTAG TAAGACACTG
    GCAGCCAATG AGCCACATCT GACCTTAGAA TTTTTGGAAG AGTGTATTTC TGGATTTAGC
    AAGTCTAGTA TTGAATTGAA ACACCTTTGT TTGGAATACA TGACTCCGTG GCTGTCAAAT
    CTAGTCCGTT TTTGTAAGCA CAATGATGAT GCCAAAAGAC AGAGAGTTAC TGCTATTCTT
    GATAAGCTGA TAACAATGAC CATAAATGAG AAACAGATGT ACCCATCTAT CCAGGCCAAA
    ATATGGGGGA GCCTTGGGCA GATTACAGAT CTCCTTGATG TTGTATTAGA CAGTTTCATC
    AAAACCAGTG CAACAGGTGG CTTGGGATCA ATAAAAGCTG AGGTGATGGC AGATACTGCT
    GTAGCTTTGG CTTCTGGAAA CGTGAAATTG GTTTCAAGCA AGGTTATTGG AAGGATGTGC
    AAAATAATTG ACAAGACATG TTTATCTCCA ACTCCTACCT TAGAGCAACA TCTTATGTGG
    GACGATATTG CTATTCTAGC ACGCTACATG CTGATGCTGT CCTTCAACAA TTCCCTTGAT
    GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT GTTGTTACTT TCTTAGTAGC TACAGGGCCA
    CTCTCCCTTA GAGCTTCCAC ACATGGATTG GTCATTAATA TCATTCACTC TCTGTGTACT
    TGTTCACAGC TTCATTTTAG TGAAGAGACA AAGCAAGTTT TGAGACTCAG TTTGACAGAG
    TTCTCATTAC CCAAGTTTTA CCTGCTGTTT GGCATTAGTA AAGTCAAGTC AGCTGCCGTC
    ATTGCCTTTC GCTCCAGTTA CCGGGACAGG TCATTCTCTC CTGGCTCCTA TGAAAGGGAG
    ACTTTTGCTT TGACATCCTT GGAAACAGTC ACAGAAGCTT TGTTGGAGAT TATGGAGGCA
    TGTATGAGAG ATATTCCAAC ATGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAAAGA
    TTTGCATTCC AATATAATCC ATCCCTGCAA CCAAGAGCTC TTGTTGTCTT TGGCTGTATT
    AGCAAACGAG TGTCTCATGG GCAGATAAAA CAGATTATCC GTATTCTTAG CAAGGCACTT
    GAGAGTTGCT TAAAAGGACC TGATACTTAC AACAGTCAAG TTCTGATAGA AGCTACAGTA
    ATAGCACTAA CCAAATTACA ACCACTTCTT AATAAGGACT CCCCTCTGCA CAAAGCCCTC
    TTTTGGGTGG CTGTGGCTGT GCTACAGCTC GATGAGGTCA ACCTGTATTC AGCAGGCAGT
    GCGCTTCTGG AACAAAACCT GCACACCTTA GACAGTCTGC GGGTATTCAA TGACAAGAGT
    CCAGAGGAAG TATTTATGGC AATCCGGAAT CCTCTGGAAT GGCACTGCAA ACAAATGGAT
    CATTTTGTTG GACTCAATTT CAACTCTAAC TTTAACTTCG CACTGGTTGG ACACCTCTTA
    AAAGGGTACA GGCATCCTTC ACCTGCCATT GTGGCAAGAA CAGTCAGAAT TTTGCATACA
    CTACTAACTC TGGTGAACAA ACATAGAAAC TGTGACAAAT TTGAGGTGAA CACACAGAGC
    GTGGCTTATT TAGCAGCCTT ACTCACAGTG TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA
    AAACATAGAA AATCTCTTCT TCTTACTGAT ATTTCAATGG AAAATGTTCC TATGGATACG
    TATCCCATCC ATCATGGCGA CCCTAGCTAT AGGACGCTAA AGGAGAACCA GCCGTGGTCA
    TCTCCCAAAG GTTCTGAAGG GTACCTTGCA GCCTCCTATC CAGCCGTGGG CCAGACCAGT
    CCCCGAGCCA GGAAATCCAT GAGTTTGGAC ATGGGGCAGC CTTCTCAGGC CAATACGAAG
    AAGTTGCTGG GAACAAGGAA AAGTTTTGAT CACTTGATAT CAGACACAAA GGCTCCTAAA
    AGGCAAGAAA TGGAATCAGG GATCACAACA CCCCCCAAAA TGAGGAGAGT AGCAGAAACT
    GATTATGAAA TGGAAACTCA GAGGATTTCC TCATCACAGC AACACCCACA TTTACGCAAA
    GTTTCAGTGT CTGAATCAAA TGTTCTCTTG GATGAAGAAG TACTTACTGA TCCGAAGATC
    CAAGCACTTC TTCTTACTGT TCTGGCTACA CTGGTAAAAT ACACCACAGA TGAGTTTGAT
    CAGCGAATTC TTTATGAATA CTTAGCAGAA GCCAGTGTTG TTTTCCCCAA GGTTTTTCCT
    GTTGTGCACA ATTTGTTGGA CTCTAAGATC AACACCTTGT TGTCATTGTG CCAAGATCCA
    AATTTGTTAA ATCCAATCCA TGGAATCGTG CAGAGTGTGG TATACCATGA AGAATCCCCA
    CCTCAGTACC AAACATCTTA TCTGCAAAGT TTTGGTTTTA ATGGCTTATG GCGGTTTGCA
    GGACCCTTTT CAAAGCAAAC ACAAATCCCA GACTATGCTG AGCTTATTGT TAAGTTTCTT
    GATGCCTTGA TTGACACGTA TTTGCCTGGA ATTGATGAAG AAACCAGTGA GGAGTCCCTC
    CTGACACCCA CTTCTCCTTA TCCTCCTGCA GTGCAGAGCC AGCTTAGTAT TACTGCTAAT
    CTTAACCTTT CTAATTCCAT GACCTCACTT GCAACTTCCC AGCATTCCCC AGGAATCGAC
    AAGGAGAACG TTGAACTCTC CCCAACCACT GGGCACTGTA ACAGTGGACG GACTCGCCAT
    GGATCCGCAA GCCAAGTGCA GAAGCAAAGA AGCGCTGGCA GTTTCAAACG TAATAGCATT
    AAGAAGATCG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290774
    Clone ID Related Accession (Same CDS sequence) XM_022423325.1
    Accession Version XM_022423325.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8352bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofibromin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1321
    1381
    1441
    1501
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    1621
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    1981
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    8341
    ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
    GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA
    ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA
    AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC
    AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT
    TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT
    GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT
    GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT
    CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA
    AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG
    CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT
    GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC
    GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC
    TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG
    TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC
    AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC
    CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC
    TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT
    GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT
    GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC
    CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT
    AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG
    AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA
    ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT
    ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC
    ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG
    CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC
    AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGGCCGAT
    AGAAGTTCCT GTCACTTTCT CTTCCTTTAT GGGGTAGGAT GTGATGTTCC TTCAAGTGGA
    ACTACTAGTC AGATGTCCAT GGATCATGAA GAATTACTAC GTTCTTGTAC TCCTGGAGCC
    TCTCTCCGGA AGGGAAAAGG AAACTCATCC ATGGATAGTG CAGCAGGGTG CAGCGGTACT
    CCGCCAATAT GCCGACAAGC TCAGACTAAA CTAGAAGTGG CCCTGTACAT GTTTCTGTGG
    AGCCCTGACA CTGAAGCTGT TCTGGTTGCC ATGTCCTGTT TCCGTCACCT TTGTGAGGAA
    GCAGATATCC GGTGTGGGGT GGACGAAGTG TCGGTGCACA ACTTCTTGCC CAACTATAAC
    ACATTTATGG AATTTGCCTC TGTCAGCAAT ATGATGTCAA CAGGAAGAGC AGCACTTCAG
    AAAAGAGTGA TGGCACTACT AAGGCGCATT GAGCATCCCA CTGCAGGAAA TACCGAGGCT
    TGGGAAGATA CACATGCGAA GTGGGAACAA GCTACAAAAC TAATCCTCAA CTACCCAAAA
    GCTAAAATGG AAGATGGCCA GGCTGCTGAA AGCCTTCACA AGACCATTGT TAAGAGGCGA
    ATGTCTCATG TCAGTGGAGG AGGATCCATA GATTTGTCTG ACACGGACTC CCTACAGGAA
    TGGATCAACA TGACTGGCTT CCTCTGTGCC CTTGGGGGAG TGTGCCTGCA GCAGAGAAGC
    AATTCTGGCT TGGCAACCTA TAGCCCACCC ATGGGCCCAG TCAGTGAACG TAAGGGTTCC
    ATGATTTCAG TGATGTCTTC AGAGGGAAAT GCAGATACAC CTGTCAGCAA ATTTATGGAT
    CGGCTGTTAT CCTTAATGGT GTGTAACCAT GAGAAAGTGG GACTTCAAAT ACGGACCAAT
    GTTAAGGATC TGGTGGGTCT AGAATTGAGT CCTGCTCTTT ATCCAATGCT ATTTAACAAA
    TTGAAGAATA CCATCAGCAA GTTTTTTGAC TCCCAAGGAC AGGTTTTATT GACTGACACC
    AATACTCAGT TTGTGGAGCA AACCATAGCT ATAATGAAGA ACTTACTAGA TAATCATACT
    GAAGGCAGCT CTGAACACCT GGGGCAAGCT AGCATTGAAA CAATGATGTT AAATCTAGTC
    AGATATGTTC GTGTGCTTGG GAATATGGTC CATGCAATTC AAATAAAAAC GAAACTCTGT
    CAGTTAGTCG AGGTGATGAT GGCGAGGAGA GATGACCTCT CATTTTGTCA AGAGATGAAA
    TTTAGGAATA AGATGGTAGA ATACCTGACA GACTGGGTTA TGGGAACATC CAACCAGGCA
    GCAGATGATG ATGTAAAATG TCTTACAAGA GACTTGGACC AGGCAAGCAT GGAAGCAGTA
    GTTTCACTCC TGGCTGGTCT TCCACTGCAG CCGGAGGAAG GAGATGGTGT GGAATTGATG
    GAAGCCAAGT CACAGTTATT TCTTAAGTAT TTCACATTGT TCATGAACCT ATTGAATGAC
    TGTAGTGAAG TTGAAGATGA AAATGCACAA ACAGGTGGCA GGAAACGTGG CATGTCTCGG
    AGGCTGGCAT CACTGAGGCA CTGTACAGTC CTTGCAATGT CAAACTTACT CAATGCTAAT
    GTGGACAGTG GCCTCATGCA TTCCATAGGT TTAGGTTATC ACAAAGATCT TCAGACAAGA
    GCTACATTTA TGGAAGTTCT GACAAAAATC CTCCAACAAG GCACAGAATT TGACACACTT
    GCGGAAACAG TGCTGGCCGA CCGGTTTGAG AGGCTGGTGG AGTTGGTGAC GATGATGGGA
    GACCAGGGAG AGCTCCCAAT AGCCATGGCT CTGGCCAACG TGGTCCCGTG TTCCCAGTGG
    GATGAACTAG CTCGAGTTTT GGTGACTCTG TTTGATTCTC GGCATTTACT CTACCAATTG
    CTCTGGAACA TGTTTTCTAA GGAAGTAGAA TTGGCAGACT CCATGCAGAC TCTTTTCCGA
    GGAAACAGCT TAGCCAGTAA AATAATGACC TTCTGTTTCA AGGTATATGG TGCTACCTAC
    CTACAAAAAC TCTTGGATCC GTTATTACGA ATTGTGATCA CATCCTCTGA TTGGCAGCAT
    GTTAGCTTTG AAGTGGATCC TACCAGATTA GAACAGTCAG AGAGCCTTGA GGAAAACCAA
    CGGAACCTTC TTCAGATGAC TGAAAAGTTC TTCCATGCCA TCATCAGTTC CTCCTCAGAA
    TTCCCCCCTC AGCTTCGAAG TGTGTGCCAC TGTTTATACC AGGCAACTTG CCACTCCCTA
    CTGAATAAAG CTACAGTAAA AGAAAAAAAG GAAAACAAAA AATCAGTGGT TAGCCAGCGT
    TTCCCTCAGA ACAGCATCGG TGCAGTGGGA AGTGCCATGT TCCTCAGATT TATCAATCCT
    GCCATTGTCT CACCGTACGA AGCAGGGATT TTAGATAAAA AGCCACCACC TAGAATTGAA
    AGGGGCTTGA AGTTAATGTC AAAGATACTT CAGAGTATTG CCAATCATGT TCTTTTCACA
    AAAGAAGAGC ATATGCGGCC TTTTAATGAT TTTGTGAAGA GCAACTTTGA TGCAGCACGG
    AGGTTTTTCC TTGATATAGC ATCTGATTGT CCTACAAGTG ATACAGTAAA TCATAGTCTT
    TCCTTCATCA GTGATGGCAA TGTGCTTGCT TTACATCGTC TACTCTGGAA CAATCAAGAG
    AAAATTGGCC AGTATCTTTC CAGCAACAGG GATCACAAAG CTGTTGGAAG ACGACCTTTC
    GATAAGATGG CAACACTTCT TGCATACCTG GGTCCTCCAG AGCACAAACC TGTGGCAGAT
    ACACACTGGT CCAGCCTTAA CCTTACCAGT TCAAAGTTTG AGGAATTTAT GACTAGGCAT
    CAAGTACATG AAAAAGAAGA GTTCAAGGCT TTGAAAACAT TAAGTATTTT CTACCAAGCA
    GGGACTTCCA AAGCTGGGAA TCCTATATTT TATTATGTTG CACGGAGGTT TAAAACTGGT
    CAAATCAATG GCGATTTGCT GATATACCAT GTCTTGCTGA CTTTAAAGCC ATATTATGCA
    AAGCCATATG AAATTGTAGT GGACCTTACG CATACTGGGC CGAGCAATCG CTTTAAAACA
    GACTTTCTCT CCAAGTGGTT TGTTGTTTTT CCTGGCTTCG CTTATGACAA TGTCTCTGCA
    GTCTATATCT ATAACTGTAA CTCCTGGGTC AGAGAGTACA CCAAGTATCA CGAGCGGCTG
    CTGACTGGCC TCAAAGGCAG CAAAAGGCTC ATCTTCATAG ACTGTCCTGG GAAACTGGCT
    GAGCACATAG AGCATGAACA ACAGAAACTG CCTGCTGCCA CACTGGCTTT AGAAGAGGAC
    CTGAAGGTAT TCCACAATGC TCTCAAGCTA GCTCACAAAG ACACCAAGGT TTCCATTAAA
    GTTGGTTCTA CTGCTGTTCA AGTAACCTCA GCAGAGCGAA CAAAAGTCCT GGGGCAATCG
    GTCTTTCTAA ATGATATTTA TTATGCTTCG GAAATTGAAG AGATCTGCCT GGTAGATGAG
    AACCAGTTTA CCTTAACCAT TGCAAACCAG GGCACACCAC TCACCTTCAT GCACCAGGAG
    TGTGAAGCCA TTGTTCAGTC TATCATCCAC ATCCGGACAC GCTGGGAGCT GTCACAACCT
    GACTCCATCC CCCAACACAC CAAGATTCGG CCAAAAGATG TCCCTGGGAC GCTGCTCAAT
    ATTGCGTTAC TTAATTTAGG CAGTTCAGAC CCTAGTTTAC GGTCAGCTGC CTATAATCTT
    CTGTGTGCCT TAACTTGTAC CTTCAATTTA AAAATCGAGG GCCAATTACT AGAGACATCA
    GGTTTATGCA TCCCTGCCAA CAACACCCTC TTTATTGTCT CTATTAGTAA GACACTGGCA
    GCCAATGAGC CACATCTGAC CTTAGAATTT TTGGAAGAGT GTATTTCTGG ATTTAGCAAG
    TCTAGTATTG AATTGAAACA CCTTTGTTTG GAATACATGA CTCCGTGGCT GTCAAATCTA
    GTCCGTTTTT GTAAGCACAA TGATGATGCC AAAAGACAGA GAGTTACTGC TATTCTTGAT
    AAGCTGATAA CAATGACCAT AAATGAGAAA CAGATGTACC CATCTATCCA GGCCAAAATA
    TGGGGGAGCC TTGGGCAGAT TACAGATCTC CTTGATGTTG TATTAGACAG TTTCATCAAA
    ACCAGTGCAA CAGGTGGCTT GGGATCAATA AAAGCTGAGG TGATGGCAGA TACTGCTGTA
    GCTTTGGCTT CTGGAAACGT GAAATTGGTT TCAAGCAAGG TTATTGGAAG GATGTGCAAA
    ATAATTGACA AGACATGTTT ATCTCCAACT CCTACCTTAG AGCAACATCT TATGTGGGAC
    GATATTGCTA TTCTAGCACG CTACATGCTG ATGCTGTCCT TCAACAATTC CCTTGATGTG
    GCAGCTCATC TCCCCTACCT CTTCCATGTT GTTACTTTCT TAGTAGCTAC AGGGCCACTC
    TCCCTTAGAG CTTCCACACA TGGATTGGTC ATTAATATCA TTCACTCTCT GTGTACTTGT
    TCACAGCTTC ATTTTAGTGA AGAGACAAAG CAAGTTTTGA GACTCAGTTT GACAGAGTTC
    TCATTACCCA AGTTTTACCT GCTGTTTGGC ATTAGTAAAG TCAAGTCAGC TGCCGTCATT
    GCCTTTCGCT CCAGTTACCG GGACAGGTCA TTCTCTCCTG GCTCCTATGA AAGGGAGACT
    TTTGCTTTGA CATCCTTGGA AACAGTCACA GAAGCTTTGT TGGAGATTAT GGAGGCATGT
    ATGAGAGATA TTCCAACATG CAAGTGGCTG GACCAGTGGA CAGAACTAGC TCAAAGATTT
    GCATTCCAAT ATAATCCATC CCTGCAACCA AGAGCTCTTG TTGTCTTTGG CTGTATTAGC
    AAACGAGTGT CTCATGGGCA GATAAAACAG ATTATCCGTA TTCTTAGCAA GGCACTTGAG
    AGTTGCTTAA AAGGACCTGA TACTTACAAC AGTCAAGTTC TGATAGAAGC TACAGTAATA
    GCACTAACCA AATTACAACC ACTTCTTAAT AAGGACTCCC CTCTGCACAA AGCCCTCTTT
    TGGGTGGCTG TGGCTGTGCT ACAGCTCGAT GAGGTCAACC TGTATTCAGC AGGCAGTGCG
    CTTCTGGAAC AAAACCTGCA CACCTTAGAC AGTCTGCGGG TATTCAATGA CAAGAGTCCA
    GAGGAAGTAT TTATGGCAAT CCGGAATCCT CTGGAATGGC ACTGCAAACA AATGGATCAT
    TTTGTTGGAC TCAATTTCAA CTCTAACTTT AACTTCGCAC TGGTTGGACA CCTCTTAAAA
    GGGTACAGGC ATCCTTCACC TGCCATTGTG GCAAGAACAG TCAGAATTTT GCATACACTA
    CTAACTCTGG TGAACAAACA TAGAAACTGT GACAAATTTG AGGTGAACAC ACAGAGCGTG
    GCTTATTTAG CAGCCTTACT CACAGTGTCT GAGGAGGTTC GAAGTCGCTG TAGCCTAAAA
    CATAGAAAAT CTCTTCTTCT TACTGATATT TCAATGGAAA ATGTTCCTAT GGATACGTAT
    CCCATCCATC ATGGCGACCC TAGCTATAGG ACGCTAAAGG AGAACCAGCC GTGGTCATCT
    CCCAAAGGTT CTGAAGGGTA CCTTGCAGCC TCCTATCCAG CCGTGGGCCA GACCAGTCCC
    CGAGCCAGGA AATCCATGAG TTTGGACATG GGGCAGCCTT CTCAGGCCAA TACGAAGAAG
    TTGCTGGGAA CAAGGAAAAG TTTTGATCAC TTGATATCAG ACACAAAGGC TCCTAAAAGG
    CAAGAAATGG AATCAGGGAT CACAACACCC CCCAAAATGA GGAGAGTAGC AGAAACTGAT
    TATGAAATGG AAACTCAGAG GATTTCCTCA TCACAGCAAC ACCCACATTT ACGCAAAGTT
    TCAGTGTCTG AATCAAATGT TCTCTTGGAT GAAGAAGTAC TTACTGATCC GAAGATCCAA
    GCACTTCTTC TTACTGTTCT GGCTACACTG GTAAAATACA CCACAGATGA GTTTGATCAG
    CGAATTCTTT ATGAATACTT AGCAGAAGCC AGTGTTGTTT TCCCCAAGGT TTTTCCTGTT
    GTGCACAATT TGTTGGACTC TAAGATCAAC ACCTTGTTGT CATTGTGCCA AGATCCAAAT
    TTGTTAAATC CAATCCATGG AATCGTGCAG AGTGTGGTAT ACCATGAAGA ATCCCCACCT
    CAGTACCAAA CATCTTATCT GCAAAGTTTT GGTTTTAATG GCTTATGGCG GTTTGCAGGA
    CCCTTTTCAA AGCAAACACA AATCCCAGAC TATGCTGAGC TTATTGTTAA GTTTCTTGAT
    GCCTTGATTG ACACGTATTT GCCTGGAATT GATGAAGAAA CCAGTGAGGA GTCCCTCCTG
    ACACCCACTT CTCCTTATCC TCCTGCAGTG CAGAGCCAGC TTAGTATTAC TGCTAATCTT
    AACCTTTCTA ATTCCATGAC CTCACTTGCA ACTTCCCAGC ATTCCCCAGG AATCGACAAG
    GAGAACGTTG AACTCTCCCC AACCACTGGG CACTGTAACA GTGGACGGAC TCGCCATGGA
    TCCGCAAGCC AAGTGCAGAA GCAAAGAAGC GCTGGCAGTT TCAAACGTAA TAGCATTAAG
    AAGATCGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279033.1
    CDS148..8499
    Translation

    Target ORF information:

    RefSeq Version XM_022423325.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423325.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
    GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA
    ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA
    AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC
    AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT
    TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT
    GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT
    GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT
    CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA
    AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG
    CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT
    GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC
    GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC
    TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG
    TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC
    AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC
    CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC
    TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT
    GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT
    GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC
    CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT
    AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG
    AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA
    ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT
    ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC
    ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG
    CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC
    AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGGCCGAT
    AGAAGTTCCT GTCACTTTCT CTTCCTTTAT GGGGTAGGAT GTGATGTTCC TTCAAGTGGA
    ACTACTAGTC AGATGTCCAT GGATCATGAA GAATTACTAC GTTCTTGTAC TCCTGGAGCC
    TCTCTCCGGA AGGGAAAAGG AAACTCATCC ATGGATAGTG CAGCAGGGTG CAGCGGTACT
    CCGCCAATAT GCCGACAAGC TCAGACTAAA CTAGAAGTGG CCCTGTACAT GTTTCTGTGG
    AGCCCTGACA CTGAAGCTGT TCTGGTTGCC ATGTCCTGTT TCCGTCACCT TTGTGAGGAA
    GCAGATATCC GGTGTGGGGT GGACGAAGTG TCGGTGCACA ACTTCTTGCC CAACTATAAC
    ACATTTATGG AATTTGCCTC TGTCAGCAAT ATGATGTCAA CAGGAAGAGC AGCACTTCAG
    AAAAGAGTGA TGGCACTACT AAGGCGCATT GAGCATCCCA CTGCAGGAAA TACCGAGGCT
    TGGGAAGATA CACATGCGAA GTGGGAACAA GCTACAAAAC TAATCCTCAA CTACCCAAAA
    GCTAAAATGG AAGATGGCCA GGCTGCTGAA AGCCTTCACA AGACCATTGT TAAGAGGCGA
    ATGTCTCATG TCAGTGGAGG AGGATCCATA GATTTGTCTG ACACGGACTC CCTACAGGAA
    TGGATCAACA TGACTGGCTT CCTCTGTGCC CTTGGGGGAG TGTGCCTGCA GCAGAGAAGC
    AATTCTGGCT TGGCAACCTA TAGCCCACCC ATGGGCCCAG TCAGTGAACG TAAGGGTTCC
    ATGATTTCAG TGATGTCTTC AGAGGGAAAT GCAGATACAC CTGTCAGCAA ATTTATGGAT
    CGGCTGTTAT CCTTAATGGT GTGTAACCAT GAGAAAGTGG GACTTCAAAT ACGGACCAAT
    GTTAAGGATC TGGTGGGTCT AGAATTGAGT CCTGCTCTTT ATCCAATGCT ATTTAACAAA
    TTGAAGAATA CCATCAGCAA GTTTTTTGAC TCCCAAGGAC AGGTTTTATT GACTGACACC
    AATACTCAGT TTGTGGAGCA AACCATAGCT ATAATGAAGA ACTTACTAGA TAATCATACT
    GAAGGCAGCT CTGAACACCT GGGGCAAGCT AGCATTGAAA CAATGATGTT AAATCTAGTC
    AGATATGTTC GTGTGCTTGG GAATATGGTC CATGCAATTC AAATAAAAAC GAAACTCTGT
    CAGTTAGTCG AGGTGATGAT GGCGAGGAGA GATGACCTCT CATTTTGTCA AGAGATGAAA
    TTTAGGAATA AGATGGTAGA ATACCTGACA GACTGGGTTA TGGGAACATC CAACCAGGCA
    GCAGATGATG ATGTAAAATG TCTTACAAGA GACTTGGACC AGGCAAGCAT GGAAGCAGTA
    GTTTCACTCC TGGCTGGTCT TCCACTGCAG CCGGAGGAAG GAGATGGTGT GGAATTGATG
    GAAGCCAAGT CACAGTTATT TCTTAAGTAT TTCACATTGT TCATGAACCT ATTGAATGAC
    TGTAGTGAAG TTGAAGATGA AAATGCACAA ACAGGTGGCA GGAAACGTGG CATGTCTCGG
    AGGCTGGCAT CACTGAGGCA CTGTACAGTC CTTGCAATGT CAAACTTACT CAATGCTAAT
    GTGGACAGTG GCCTCATGCA TTCCATAGGT TTAGGTTATC ACAAAGATCT TCAGACAAGA
    GCTACATTTA TGGAAGTTCT GACAAAAATC CTCCAACAAG GCACAGAATT TGACACACTT
    GCGGAAACAG TGCTGGCCGA CCGGTTTGAG AGGCTGGTGG AGTTGGTGAC GATGATGGGA
    GACCAGGGAG AGCTCCCAAT AGCCATGGCT CTGGCCAACG TGGTCCCGTG TTCCCAGTGG
    GATGAACTAG CTCGAGTTTT GGTGACTCTG TTTGATTCTC GGCATTTACT CTACCAATTG
    CTCTGGAACA TGTTTTCTAA GGAAGTAGAA TTGGCAGACT CCATGCAGAC TCTTTTCCGA
    GGAAACAGCT TAGCCAGTAA AATAATGACC TTCTGTTTCA AGGTATATGG TGCTACCTAC
    CTACAAAAAC TCTTGGATCC GTTATTACGA ATTGTGATCA CATCCTCTGA TTGGCAGCAT
    GTTAGCTTTG AAGTGGATCC TACCAGATTA GAACAGTCAG AGAGCCTTGA GGAAAACCAA
    CGGAACCTTC TTCAGATGAC TGAAAAGTTC TTCCATGCCA TCATCAGTTC CTCCTCAGAA
    TTCCCCCCTC AGCTTCGAAG TGTGTGCCAC TGTTTATACC AGGCAACTTG CCACTCCCTA
    CTGAATAAAG CTACAGTAAA AGAAAAAAAG GAAAACAAAA AATCAGTGGT TAGCCAGCGT
    TTCCCTCAGA ACAGCATCGG TGCAGTGGGA AGTGCCATGT TCCTCAGATT TATCAATCCT
    GCCATTGTCT CACCGTACGA AGCAGGGATT TTAGATAAAA AGCCACCACC TAGAATTGAA
    AGGGGCTTGA AGTTAATGTC AAAGATACTT CAGAGTATTG CCAATCATGT TCTTTTCACA
    AAAGAAGAGC ATATGCGGCC TTTTAATGAT TTTGTGAAGA GCAACTTTGA TGCAGCACGG
    AGGTTTTTCC TTGATATAGC ATCTGATTGT CCTACAAGTG ATACAGTAAA TCATAGTCTT
    TCCTTCATCA GTGATGGCAA TGTGCTTGCT TTACATCGTC TACTCTGGAA CAATCAAGAG
    AAAATTGGCC AGTATCTTTC CAGCAACAGG GATCACAAAG CTGTTGGAAG ACGACCTTTC
    GATAAGATGG CAACACTTCT TGCATACCTG GGTCCTCCAG AGCACAAACC TGTGGCAGAT
    ACACACTGGT CCAGCCTTAA CCTTACCAGT TCAAAGTTTG AGGAATTTAT GACTAGGCAT
    CAAGTACATG AAAAAGAAGA GTTCAAGGCT TTGAAAACAT TAAGTATTTT CTACCAAGCA
    GGGACTTCCA AAGCTGGGAA TCCTATATTT TATTATGTTG CACGGAGGTT TAAAACTGGT
    CAAATCAATG GCGATTTGCT GATATACCAT GTCTTGCTGA CTTTAAAGCC ATATTATGCA
    AAGCCATATG AAATTGTAGT GGACCTTACG CATACTGGGC CGAGCAATCG CTTTAAAACA
    GACTTTCTCT CCAAGTGGTT TGTTGTTTTT CCTGGCTTCG CTTATGACAA TGTCTCTGCA
    GTCTATATCT ATAACTGTAA CTCCTGGGTC AGAGAGTACA CCAAGTATCA CGAGCGGCTG
    CTGACTGGCC TCAAAGGCAG CAAAAGGCTC ATCTTCATAG ACTGTCCTGG GAAACTGGCT
    GAGCACATAG AGCATGAACA ACAGAAACTG CCTGCTGCCA CACTGGCTTT AGAAGAGGAC
    CTGAAGGTAT TCCACAATGC TCTCAAGCTA GCTCACAAAG ACACCAAGGT TTCCATTAAA
    GTTGGTTCTA CTGCTGTTCA AGTAACCTCA GCAGAGCGAA CAAAAGTCCT GGGGCAATCG
    GTCTTTCTAA ATGATATTTA TTATGCTTCG GAAATTGAAG AGATCTGCCT GGTAGATGAG
    AACCAGTTTA CCTTAACCAT TGCAAACCAG GGCACACCAC TCACCTTCAT GCACCAGGAG
    TGTGAAGCCA TTGTTCAGTC TATCATCCAC ATCCGGACAC GCTGGGAGCT GTCACAACCT
    GACTCCATCC CCCAACACAC CAAGATTCGG CCAAAAGATG TCCCTGGGAC GCTGCTCAAT
    ATTGCGTTAC TTAATTTAGG CAGTTCAGAC CCTAGTTTAC GGTCAGCTGC CTATAATCTT
    CTGTGTGCCT TAACTTGTAC CTTCAATTTA AAAATCGAGG GCCAATTACT AGAGACATCA
    GGTTTATGCA TCCCTGCCAA CAACACCCTC TTTATTGTCT CTATTAGTAA GACACTGGCA
    GCCAATGAGC CACATCTGAC CTTAGAATTT TTGGAAGAGT GTATTTCTGG ATTTAGCAAG
    TCTAGTATTG AATTGAAACA CCTTTGTTTG GAATACATGA CTCCGTGGCT GTCAAATCTA
    GTCCGTTTTT GTAAGCACAA TGATGATGCC AAAAGACAGA GAGTTACTGC TATTCTTGAT
    AAGCTGATAA CAATGACCAT AAATGAGAAA CAGATGTACC CATCTATCCA GGCCAAAATA
    TGGGGGAGCC TTGGGCAGAT TACAGATCTC CTTGATGTTG TATTAGACAG TTTCATCAAA
    ACCAGTGCAA CAGGTGGCTT GGGATCAATA AAAGCTGAGG TGATGGCAGA TACTGCTGTA
    GCTTTGGCTT CTGGAAACGT GAAATTGGTT TCAAGCAAGG TTATTGGAAG GATGTGCAAA
    ATAATTGACA AGACATGTTT ATCTCCAACT CCTACCTTAG AGCAACATCT TATGTGGGAC
    GATATTGCTA TTCTAGCACG CTACATGCTG ATGCTGTCCT TCAACAATTC CCTTGATGTG
    GCAGCTCATC TCCCCTACCT CTTCCATGTT GTTACTTTCT TAGTAGCTAC AGGGCCACTC
    TCCCTTAGAG CTTCCACACA TGGATTGGTC ATTAATATCA TTCACTCTCT GTGTACTTGT
    TCACAGCTTC ATTTTAGTGA AGAGACAAAG CAAGTTTTGA GACTCAGTTT GACAGAGTTC
    TCATTACCCA AGTTTTACCT GCTGTTTGGC ATTAGTAAAG TCAAGTCAGC TGCCGTCATT
    GCCTTTCGCT CCAGTTACCG GGACAGGTCA TTCTCTCCTG GCTCCTATGA AAGGGAGACT
    TTTGCTTTGA CATCCTTGGA AACAGTCACA GAAGCTTTGT TGGAGATTAT GGAGGCATGT
    ATGAGAGATA TTCCAACATG CAAGTGGCTG GACCAGTGGA CAGAACTAGC TCAAAGATTT
    GCATTCCAAT ATAATCCATC CCTGCAACCA AGAGCTCTTG TTGTCTTTGG CTGTATTAGC
    AAACGAGTGT CTCATGGGCA GATAAAACAG ATTATCCGTA TTCTTAGCAA GGCACTTGAG
    AGTTGCTTAA AAGGACCTGA TACTTACAAC AGTCAAGTTC TGATAGAAGC TACAGTAATA
    GCACTAACCA AATTACAACC ACTTCTTAAT AAGGACTCCC CTCTGCACAA AGCCCTCTTT
    TGGGTGGCTG TGGCTGTGCT ACAGCTCGAT GAGGTCAACC TGTATTCAGC AGGCAGTGCG
    CTTCTGGAAC AAAACCTGCA CACCTTAGAC AGTCTGCGGG TATTCAATGA CAAGAGTCCA
    GAGGAAGTAT TTATGGCAAT CCGGAATCCT CTGGAATGGC ACTGCAAACA AATGGATCAT
    TTTGTTGGAC TCAATTTCAA CTCTAACTTT AACTTCGCAC TGGTTGGACA CCTCTTAAAA
    GGGTACAGGC ATCCTTCACC TGCCATTGTG GCAAGAACAG TCAGAATTTT GCATACACTA
    CTAACTCTGG TGAACAAACA TAGAAACTGT GACAAATTTG AGGTGAACAC ACAGAGCGTG
    GCTTATTTAG CAGCCTTACT CACAGTGTCT GAGGAGGTTC GAAGTCGCTG TAGCCTAAAA
    CATAGAAAAT CTCTTCTTCT TACTGATATT TCAATGGAAA ATGTTCCTAT GGATACGTAT
    CCCATCCATC ATGGCGACCC TAGCTATAGG ACGCTAAAGG AGAACCAGCC GTGGTCATCT
    CCCAAAGGTT CTGAAGGGTA CCTTGCAGCC TCCTATCCAG CCGTGGGCCA GACCAGTCCC
    CGAGCCAGGA AATCCATGAG TTTGGACATG GGGCAGCCTT CTCAGGCCAA TACGAAGAAG
    TTGCTGGGAA CAAGGAAAAG TTTTGATCAC TTGATATCAG ACACAAAGGC TCCTAAAAGG
    CAAGAAATGG AATCAGGGAT CACAACACCC CCCAAAATGA GGAGAGTAGC AGAAACTGAT
    TATGAAATGG AAACTCAGAG GATTTCCTCA TCACAGCAAC ACCCACATTT ACGCAAAGTT
    TCAGTGTCTG AATCAAATGT TCTCTTGGAT GAAGAAGTAC TTACTGATCC GAAGATCCAA
    GCACTTCTTC TTACTGTTCT GGCTACACTG GTAAAATACA CCACAGATGA GTTTGATCAG
    CGAATTCTTT ATGAATACTT AGCAGAAGCC AGTGTTGTTT TCCCCAAGGT TTTTCCTGTT
    GTGCACAATT TGTTGGACTC TAAGATCAAC ACCTTGTTGT CATTGTGCCA AGATCCAAAT
    TTGTTAAATC CAATCCATGG AATCGTGCAG AGTGTGGTAT ACCATGAAGA ATCCCCACCT
    CAGTACCAAA CATCTTATCT GCAAAGTTTT GGTTTTAATG GCTTATGGCG GTTTGCAGGA
    CCCTTTTCAA AGCAAACACA AATCCCAGAC TATGCTGAGC TTATTGTTAA GTTTCTTGAT
    GCCTTGATTG ACACGTATTT GCCTGGAATT GATGAAGAAA CCAGTGAGGA GTCCCTCCTG
    ACACCCACTT CTCCTTATCC TCCTGCAGTG CAGAGCCAGC TTAGTATTAC TGCTAATCTT
    AACCTTTCTA ATTCCATGAC CTCACTTGCA ACTTCCCAGC ATTCCCCAGG AATCGACAAG
    GAGAACGTTG AACTCTCCCC AACCACTGGG CACTGTAACA GTGGACGGAC TCGCCATGGA
    TCCGCAAGCC AAGTGCAGAA GCAAAGAAGC GCTGGCAGTT TCAAACGTAA TAGCATTAAG
    AAGATCGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290775
    Clone ID Related Accession (Same CDS sequence) XM_022423326.1
    Accession Version XM_022423326.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofibromin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
    GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA
    ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA
    AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC
    AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT
    TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT
    GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT
    GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT
    CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA
    AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG
    CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT
    GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC
    GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC
    TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG
    TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC
    AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC
    CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC
    TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATT CTGCATTGGA TTGGTGGCCT
    AAGATTGATG CTGTATATTG TCACTCAGTT GAGCTTCGAA ACATGTTTGG TGAAACACTT
    CATAAAGCAG TGCAGGGTTG TGGAGCACAC CCAGCAATAC GAATGGCACC GAGTCTTACA
    TTTAAAGAAA AAGTAACAAG CCTTAAATTT AAAGAAAAAC CTACAGACCT GGAGACAAGA
    AGCTATAAAT ATCTTCTCTT ATCCATGGTG AAACTAATTC ATGCAGATCC AAAGCTCTTG
    CTTTGTAATC CAAGAAAACA GGGACCTGAA ACCCAAGGCA GTACAGCGGA GTTAATTACA
    GGACTTGTCC AGCTGGTCCC TCAGTCACAT ATGCCAGAGA TTGCTCAGGA AGCAATGGAG
    GCTCTGCTGG TTCTTCATCA GTTAGACAGC ATTGATTTGT GGAATCCTGA TGCTCCTGTA
    GAAACATTTT GGGAGATTAG CTCTCAAATG CTTTTTTACA TCTGCAAGAA ATTAACTAGC
    CATCAGATGC TTAGTAGCAC AGAAATTCTC AAATGGTTGA GGGAGATTTT GATCTGCAGG
    AATAAATTTC TTCTTAAAAA TAAGCAGGCC GATAGAAGTT CCTGTCACTT TCTCTTCCTT
    TATGGGGTAG GATGTGATGT TCCTTCAAGT GGAACTACTA GTCAGATGTC CATGGATCAT
    GAAGAATTAC TACGTTCTTG TACTCCTGGA GCCTCTCTCC GGAAGGGAAA AGGAAACTCA
    TCCATGGATA GTGCAGCAGG GTGCAGCGGT ACTCCGCCAA TATGCCGACA AGCTCAGACT
    AAACTAGAAG TGGCCCTGTA CATGTTTCTG TGGAGCCCTG ACACTGAAGC TGTTCTGGTT
    GCCATGTCCT GTTTCCGTCA CCTTTGTGAG GAAGCAGATA TCCGGTGTGG GGTGGACGAA
    GTGTCGGTGC ACAACTTCTT GCCCAACTAT AACACATTTA TGGAATTTGC CTCTGTCAGC
    AATATGATGT CAACAGGAAG AGCAGCACTT CAGAAAAGAG TGATGGCACT ACTAAGGCGC
    ATTGAGCATC CCACTGCAGG AAATACCGAG GCTTGGGAAG ATACACATGC GAAGTGGGAA
    CAAGCTACAA AACTAATCCT CAACTACCCA AAAGCTAAAA TGGAAGATGG CCAGGCTGCT
    GAAAGCCTTC ACAAGACCAT TGTTAAGAGG CGAATGTCTC ATGTCAGTGG AGGAGGATCC
    ATAGATTTGT CTGACACGGA CTCCCTACAG GAATGGATCA ACATGACTGG CTTCCTCTGT
    GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA AGCAATTCTG GCTTGGCAAC CTATAGCCCA
    CCCATGGGCC CAGTCAGTGA ACGTAAGGGT TCCATGATTT CAGTGATGTC TTCAGAGGGA
    AATGCAGATA CACCTGTCAG CAAATTTATG GATCGGCTGT TATCCTTAAT GGTGTGTAAC
    CATGAGAAAG TGGGACTTCA AATACGGACC AATGTTAAGG ATCTGGTGGG TCTAGAATTG
    AGTCCTGCTC TTTATCCAAT GCTATTTAAC AAATTGAAGA ATACCATCAG CAAGTTTTTT
    GACTCCCAAG GACAGGTTTT ATTGACTGAC ACCAATACTC AGTTTGTGGA GCAAACCATA
    GCTATAATGA AGAACTTACT AGATAATCAT ACTGAAGGCA GCTCTGAACA CCTGGGGCAA
    GCTAGCATTG AAACAATGAT GTTAAATCTA GTCAGATATG TTCGTGTGCT TGGGAATATG
    GTCCATGCAA TTCAAATAAA AACGAAACTC TGTCAGTTAG TCGAGGTGAT GATGGCGAGG
    AGAGATGACC TCTCATTTTG TCAAGAGATG AAATTTAGGA ATAAGATGGT AGAATACCTG
    ACAGACTGGG TTATGGGAAC ATCCAACCAG GCAGCAGATG ATGATGTAAA ATGTCTTACA
    AGAGACTTGG ACCAGGCAAG CATGGAAGCA GTAGTTTCAC TCCTGGCTGG TCTTCCACTG
    CAGCCGGAGG AAGGAGATGG TGTGGAATTG ATGGAAGCCA AGTCACAGTT ATTTCTTAAG
    TATTTCACAT TGTTCATGAA CCTATTGAAT GACTGTAGTG AAGTTGAAGA TGAAAATGCA
    CAAACAGGTG GCAGGAAACG TGGCATGTCT CGGAGGCTGG CATCACTGAG GCACTGTACA
    GTCCTTGCAA TGTCAAACTT ACTCAATGCT AATGTGGACA GTGGCCTCAT GCATTCCATA
    GGTTTAGGTT ATCACAAAGA TCTTCAGACA AGAGCTACAT TTATGGAAGT TCTGACAAAA
    ATCCTCCAAC AAGGCACAGA ATTTGACACA CTTGCGGAAA CAGTGCTGGC CGACCGGTTT
    GAGAGGCTGG TGGAGTTGGT GACGATGATG GGAGACCAGG GAGAGCTCCC AATAGCCATG
    GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG TGGGATGAAC TAGCTCGAGT TTTGGTGACT
    CTGTTTGATT CTCGGCATTT ACTCTACCAA TTGCTCTGGA ACATGTTTTC TAAGGAAGTA
    GAATTGGCAG ACTCCATGCA GACTCTTTTC CGAGGAAACA GCTTAGCCAG TAAAATAATG
    ACCTTCTGTT TCAAGGTATA TGGTGCTACC TACCTACAAA AACTCTTGGA TCCGTTATTA
    CGAATTGTGA TCACATCCTC TGATTGGCAG CATGTTAGCT TTGAAGTGGA TCCTACCAGA
    TTAGAACAGT CAGAGAGCCT TGAGGAAAAC CAACGGAACC TTCTTCAGAT GACTGAAAAG
    TTCTTCCATG CCATCATCAG TTCCTCCTCA GAATTCCCCC CTCAGCTTCG AAGTGTGTGC
    CACTGTTTAT ACCAGGCAAC TTGCCACTCC CTACTGAATA AAGCTACAGT AAAAGAAAAA
    AAGGAAAACA AAAAATCAGT GGTTAGCCAG CGTTTCCCTC AGAACAGCAT CGGTGCAGTG
    GGAAGTGCCA TGTTCCTCAG ATTTATCAAT CCTGCCATTG TCTCACCGTA CGAAGCAGGG
    ATTTTAGATA AAAAGCCACC ACCTAGAATT GAAAGGGGCT TGAAGTTAAT GTCAAAGATA
    CTTCAGAGTA TTGCCAATCA TGTTCTTTTC ACAAAAGAAG AGCATATGCG GCCTTTTAAT
    GATTTTGTGA AGAGCAACTT TGATGCAGCA CGGAGGTTTT TCCTTGATAT AGCATCTGAT
    TGTCCTACAA GTGATACAGT AAATCATAGT CTTTCCTTCA TCAGTGATGG CAATGTGCTT
    GCTTTACATC GTCTACTCTG GAACAATCAA GAGAAAATTG GCCAGTATCT TTCCAGCAAC
    AGGGATCACA AAGCTGTTGG AAGACGACCT TTCGATAAGA TGGCAACACT TCTTGCATAC
    CTGGGTCCTC CAGAGCACAA ACCTGTGGCA GATACACACT GGTCCAGCCT TAACCTTACC
    AGTTCAAAGT TTGAGGAATT TATGACTAGG CATCAAGTAC ATGAAAAAGA AGAGTTCAAG
    GCTTTGAAAA CATTAAGTAT TTTCTACCAA GCAGGGACTT CCAAAGCTGG GAATCCTATA
    TTTTATTATG TTGCACGGAG GTTTAAAACT GGTCAAATCA ATGGCGATTT GCTGATATAC
    CATGTCTTGC TGACTTTAAA GCCATATTAT GCAAAGCCAT ATGAAATTGT AGTGGACCTT
    ACGCATACTG GGCCGAGCAA TCGCTTTAAA ACAGACTTTC TCTCCAAGTG GTTTGTTGTT
    TTTCCTGGCT TCGCTTATGA CAATGTCTCT GCAGTCTATA TCTATAACTG TAACTCCTGG
    GTCAGAGAGT ACACCAAGTA TCACGAGCGG CTGCTGACTG GCCTCAAAGG CAGCAAAAGG
    CTCATCTTCA TAGACTGTCC TGGGAAACTG GCTGAGCACA TAGAGCATGA ACAACAGAAA
    CTGCCTGCTG CCACACTGGC TTTAGAAGAG GACCTGAAGG TATTCCACAA TGCTCTCAAG
    CTAGCTCACA AAGACACCAA GGTTTCCATT AAAGTTGGTT CTACTGCTGT TCAAGTAACC
    TCAGCAGAGC GAACAAAAGT CCTGGGGCAA TCGGTCTTTC TAAATGATAT TTATTATGCT
    TCGGAAATTG AAGAGATCTG CCTGGTAGAT GAGAACCAGT TTACCTTAAC CATTGCAAAC
    CAGGGCACAC CACTCACCTT CATGCACCAG GAGTGTGAAG CCATTGTTCA GTCTATCATC
    CACATCCGGA CACGCTGGGA GCTGTCACAA CCTGACTCCA TCCCCCAACA CACCAAGATT
    CGGCCAAAAG ATGTCCCTGG GACGCTGCTC AATATTGCGT TACTTAATTT AGGCAGTTCA
    GACCCTAGTT TACGGTCAGC TGCCTATAAT CTTCTGTGTG CCTTAACTTG TACCTTCAAT
    TTAAAAATCG AGGGCCAATT ACTAGAGACA TCAGGTTTAT GCATCCCTGC CAACAACACC
    CTCTTTATTG TCTCTATTAG TAAGACACTG GCAGCCAATG AGCCACATCT GACCTTAGAA
    TTTTTGGAAG AGTGTATTTC TGGATTTAGC AAGTCTAGTA TTGAATTGAA ACACCTTTGT
    TTGGAATACA TGACTCCGTG GCTGTCAAAT CTAGTCCGTT TTTGTAAGCA CAATGATGAT
    GCCAAAAGAC AGAGAGTTAC TGCTATTCTT GATAAGCTGA TAACAATGAC CATAAATGAG
    AAACAGATGT ACCCATCTAT CCAGGCCAAA ATATGGGGGA GCCTTGGGCA GATTACAGAT
    CTCCTTGATG TTGTATTAGA CAGTTTCATC AAAACCAGTG CAACAGGTGG CTTGGGATCA
    ATAAAAGCTG AGGTGATGGC AGATACTGCT GTAGCTTTGG CTTCTGGAAA CGTGAAATTG
    GTTTCAAGCA AGGTTATTGG AAGGATGTGC AAAATAATTG ACAAGACATG TTTATCTCCA
    ACTCCTACCT TAGAGCAACA TCTTATGTGG GACGATATTG CTATTCTAGC ACGCTACATG
    CTGATGCTGT CCTTCAACAA TTCCCTTGAT GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT
    GTTGTTACTT TCTTAGTAGC TACAGGGCCA CTCTCCCTTA GAGCTTCCAC ACATGGATTG
    GTCATTAATA TCATTCACTC TCTGTGTACT TGTTCACAGC TTCATTTTAG TGAAGAGACA
    AAGCAAGTTT TGAGACTCAG TTTGACAGAG TTCTCATTAC CCAAGTTTTA CCTGCTGTTT
    GGCATTAGTA AAGTCAAGTC AGCTGCCGTC ATTGCCTTTC GCTCCAGTTA CCGGGACAGG
    TCATTCTCTC CTGGCTCCTA TGAAAGGGAG ACTTTTGCTT TGACATCCTT GGAAACAGTC
    ACAGAAGCTT TGTTGGAGAT TATGGAGGCA TGTATGAGAG ATATTCCAAC ATGCAAGTGG
    CTGGACCAGT GGACAGAACT AGCTCAAAGA TTTGCATTCC AATATAATCC ATCCCTGCAA
    CCAAGAGCTC TTGTTGTCTT TGGCTGTATT AGCAAACGAG TGTCTCATGG GCAGATAAAA
    CAGATTATCC GTATTCTTAG CAAGGCACTT GAGAGTTGCT TAAAAGGACC TGATACTTAC
    AACAGTCAAG TTCTGATAGA AGCTACAGTA ATAGCACTAA CCAAATTACA ACCACTTCTT
    AATAAGGACT CCCCTCTGCA CAAAGCCCTC TTTTGGGTGG CTGTGGCTGT GCTACAGCTC
    GATGAGGTCA ACCTGTATTC AGCAGGCAGT GCGCTTCTGG AACAAAACCT GCACACCTTA
    GACAGTCTGC GGGTATTCAA TGACAAGAGT CCAGAGGAAG TATTTATGGC AATCCGGAAT
    CCTCTGGAAT GGCACTGCAA ACAAATGGAT CATTTTGTTG GACTCAATTT CAACTCTAAC
    TTTAACTTCG CACTGGTTGG ACACCTCTTA AAAGGGTACA GGCATCCTTC ACCTGCCATT
    GTGGCAAGAA CAGTCAGAAT TTTGCATACA CTACTAACTC TGGTGAACAA ACATAGAAAC
    TGTGACAAAT TTGAGGTGAA CACACAGAGC GTGGCTTATT TAGCAGCCTT ACTCACAGTG
    TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA AAACATAGAA AATCTCTTCT TCTTACTGAT
    ATTTCAATGG AAAATGTTCC TATGGATACG TATCCCATCC ATCATGGCGA CCCTAGCTAT
    AGGACGCTAA AGGAGAACCA GCCGTGGTCA TCTCCCAAAG GTTCTGAAGG GTACCTTGCA
    GCCTCCTATC CAGCCGTGGG CCAGACCAGT CCCCGAGCCA GGAAATCCAT GAGTTTGGAC
    ATGGGGCAGC CTTCTCAGGC CAATACGAAG AAGTTGCTGG GAACAAGGAA AAGTTTTGAT
    CACTTGATAT CAGACACAAA GGCTCCTAAA AGGCAAGAAA TGGAATCAGG GATCACAACA
    CCCCCCAAAA TGAGGAGAGT AGCAGAAACT GATTATGAAA TGGAAACTCA GAGGATTTCC
    TCATCACAGC AACACCCACA TTTACGCAAA GTTTCAGTGT CTGAATCAAA TGTTCTCTTG
    GATGAAGAAG TACTTACTGA TCCGAAGATC CAAGCACTTC TTCTTACTGT TCTGGCTACA
    CTGGTAAAAT ACACCACAGA TGAGTTTGAT CAGCGAATTC TTTATGAATA CTTAGCAGAA
    GCCAGTGTTG TTTTCCCCAA GGTTTTTCCT GTTGTGCACA ATTTGTTGGA CTCTAAGATC
    AACACCTTGT TGTCATTGTG CCAAGATCCA AATTTGTTAA ATCCAATCCA TGGAATCGTG
    CAGAGTGTGG TATACCATGA AGAATCCCCA CCTCAGTACC AAACATCTTA TCTGCAAAGT
    TTTGGTTTTA ATGGCTTATG GCGGTTTGCA GGACCCTTTT CAAAGCAAAC ACAAATCCCA
    GACTATGCTG AGCTTATTGT TAAGTTTCTT GATGCCTTGA TTGACACGTA TTTGCCTGGA
    ATTGATGAAG AAACCAGTGA GGAGTCCCTC CTGACACCCA CTTCTCCTTA TCCTCCTGCA
    GTGCAGAGCC AGCTTAGTAT TACTGCTAAT CTTAACCTTT CTAATTCCAT GACCTCACTT
    GCAACTTCCC AGCATTCCCC AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCAACCACT
    GGGCACTGTA ACAGTGGACG GACTCGCCAT GGATCCGCAA GCCAAGTGCA GAAGCAAAGA
    AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279034.1
    CDS148..8472
    Translation

    Target ORF information:

    RefSeq Version XM_022423326.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423326.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
    GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA
    ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA
    AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC
    AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT
    TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT
    GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT
    GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT
    CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA
    AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG
    CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT
    GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC
    GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC
    TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG
    TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC
    AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC
    CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC
    TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATT CTGCATTGGA TTGGTGGCCT
    AAGATTGATG CTGTATATTG TCACTCAGTT GAGCTTCGAA ACATGTTTGG TGAAACACTT
    CATAAAGCAG TGCAGGGTTG TGGAGCACAC CCAGCAATAC GAATGGCACC GAGTCTTACA
    TTTAAAGAAA AAGTAACAAG CCTTAAATTT AAAGAAAAAC CTACAGACCT GGAGACAAGA
    AGCTATAAAT ATCTTCTCTT ATCCATGGTG AAACTAATTC ATGCAGATCC AAAGCTCTTG
    CTTTGTAATC CAAGAAAACA GGGACCTGAA ACCCAAGGCA GTACAGCGGA GTTAATTACA
    GGACTTGTCC AGCTGGTCCC TCAGTCACAT ATGCCAGAGA TTGCTCAGGA AGCAATGGAG
    GCTCTGCTGG TTCTTCATCA GTTAGACAGC ATTGATTTGT GGAATCCTGA TGCTCCTGTA
    GAAACATTTT GGGAGATTAG CTCTCAAATG CTTTTTTACA TCTGCAAGAA ATTAACTAGC
    CATCAGATGC TTAGTAGCAC AGAAATTCTC AAATGGTTGA GGGAGATTTT GATCTGCAGG
    AATAAATTTC TTCTTAAAAA TAAGCAGGCC GATAGAAGTT CCTGTCACTT TCTCTTCCTT
    TATGGGGTAG GATGTGATGT TCCTTCAAGT GGAACTACTA GTCAGATGTC CATGGATCAT
    GAAGAATTAC TACGTTCTTG TACTCCTGGA GCCTCTCTCC GGAAGGGAAA AGGAAACTCA
    TCCATGGATA GTGCAGCAGG GTGCAGCGGT ACTCCGCCAA TATGCCGACA AGCTCAGACT
    AAACTAGAAG TGGCCCTGTA CATGTTTCTG TGGAGCCCTG ACACTGAAGC TGTTCTGGTT
    GCCATGTCCT GTTTCCGTCA CCTTTGTGAG GAAGCAGATA TCCGGTGTGG GGTGGACGAA
    GTGTCGGTGC ACAACTTCTT GCCCAACTAT AACACATTTA TGGAATTTGC CTCTGTCAGC
    AATATGATGT CAACAGGAAG AGCAGCACTT CAGAAAAGAG TGATGGCACT ACTAAGGCGC
    ATTGAGCATC CCACTGCAGG AAATACCGAG GCTTGGGAAG ATACACATGC GAAGTGGGAA
    CAAGCTACAA AACTAATCCT CAACTACCCA AAAGCTAAAA TGGAAGATGG CCAGGCTGCT
    GAAAGCCTTC ACAAGACCAT TGTTAAGAGG CGAATGTCTC ATGTCAGTGG AGGAGGATCC
    ATAGATTTGT CTGACACGGA CTCCCTACAG GAATGGATCA ACATGACTGG CTTCCTCTGT
    GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA AGCAATTCTG GCTTGGCAAC CTATAGCCCA
    CCCATGGGCC CAGTCAGTGA ACGTAAGGGT TCCATGATTT CAGTGATGTC TTCAGAGGGA
    AATGCAGATA CACCTGTCAG CAAATTTATG GATCGGCTGT TATCCTTAAT GGTGTGTAAC
    CATGAGAAAG TGGGACTTCA AATACGGACC AATGTTAAGG ATCTGGTGGG TCTAGAATTG
    AGTCCTGCTC TTTATCCAAT GCTATTTAAC AAATTGAAGA ATACCATCAG CAAGTTTTTT
    GACTCCCAAG GACAGGTTTT ATTGACTGAC ACCAATACTC AGTTTGTGGA GCAAACCATA
    GCTATAATGA AGAACTTACT AGATAATCAT ACTGAAGGCA GCTCTGAACA CCTGGGGCAA
    GCTAGCATTG AAACAATGAT GTTAAATCTA GTCAGATATG TTCGTGTGCT TGGGAATATG
    GTCCATGCAA TTCAAATAAA AACGAAACTC TGTCAGTTAG TCGAGGTGAT GATGGCGAGG
    AGAGATGACC TCTCATTTTG TCAAGAGATG AAATTTAGGA ATAAGATGGT AGAATACCTG
    ACAGACTGGG TTATGGGAAC ATCCAACCAG GCAGCAGATG ATGATGTAAA ATGTCTTACA
    AGAGACTTGG ACCAGGCAAG CATGGAAGCA GTAGTTTCAC TCCTGGCTGG TCTTCCACTG
    CAGCCGGAGG AAGGAGATGG TGTGGAATTG ATGGAAGCCA AGTCACAGTT ATTTCTTAAG
    TATTTCACAT TGTTCATGAA CCTATTGAAT GACTGTAGTG AAGTTGAAGA TGAAAATGCA
    CAAACAGGTG GCAGGAAACG TGGCATGTCT CGGAGGCTGG CATCACTGAG GCACTGTACA
    GTCCTTGCAA TGTCAAACTT ACTCAATGCT AATGTGGACA GTGGCCTCAT GCATTCCATA
    GGTTTAGGTT ATCACAAAGA TCTTCAGACA AGAGCTACAT TTATGGAAGT TCTGACAAAA
    ATCCTCCAAC AAGGCACAGA ATTTGACACA CTTGCGGAAA CAGTGCTGGC CGACCGGTTT
    GAGAGGCTGG TGGAGTTGGT GACGATGATG GGAGACCAGG GAGAGCTCCC AATAGCCATG
    GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG TGGGATGAAC TAGCTCGAGT TTTGGTGACT
    CTGTTTGATT CTCGGCATTT ACTCTACCAA TTGCTCTGGA ACATGTTTTC TAAGGAAGTA
    GAATTGGCAG ACTCCATGCA GACTCTTTTC CGAGGAAACA GCTTAGCCAG TAAAATAATG
    ACCTTCTGTT TCAAGGTATA TGGTGCTACC TACCTACAAA AACTCTTGGA TCCGTTATTA
    CGAATTGTGA TCACATCCTC TGATTGGCAG CATGTTAGCT TTGAAGTGGA TCCTACCAGA
    TTAGAACAGT CAGAGAGCCT TGAGGAAAAC CAACGGAACC TTCTTCAGAT GACTGAAAAG
    TTCTTCCATG CCATCATCAG TTCCTCCTCA GAATTCCCCC CTCAGCTTCG AAGTGTGTGC
    CACTGTTTAT ACCAGGCAAC TTGCCACTCC CTACTGAATA AAGCTACAGT AAAAGAAAAA
    AAGGAAAACA AAAAATCAGT GGTTAGCCAG CGTTTCCCTC AGAACAGCAT CGGTGCAGTG
    GGAAGTGCCA TGTTCCTCAG ATTTATCAAT CCTGCCATTG TCTCACCGTA CGAAGCAGGG
    ATTTTAGATA AAAAGCCACC ACCTAGAATT GAAAGGGGCT TGAAGTTAAT GTCAAAGATA
    CTTCAGAGTA TTGCCAATCA TGTTCTTTTC ACAAAAGAAG AGCATATGCG GCCTTTTAAT
    GATTTTGTGA AGAGCAACTT TGATGCAGCA CGGAGGTTTT TCCTTGATAT AGCATCTGAT
    TGTCCTACAA GTGATACAGT AAATCATAGT CTTTCCTTCA TCAGTGATGG CAATGTGCTT
    GCTTTACATC GTCTACTCTG GAACAATCAA GAGAAAATTG GCCAGTATCT TTCCAGCAAC
    AGGGATCACA AAGCTGTTGG AAGACGACCT TTCGATAAGA TGGCAACACT TCTTGCATAC
    CTGGGTCCTC CAGAGCACAA ACCTGTGGCA GATACACACT GGTCCAGCCT TAACCTTACC
    AGTTCAAAGT TTGAGGAATT TATGACTAGG CATCAAGTAC ATGAAAAAGA AGAGTTCAAG
    GCTTTGAAAA CATTAAGTAT TTTCTACCAA GCAGGGACTT CCAAAGCTGG GAATCCTATA
    TTTTATTATG TTGCACGGAG GTTTAAAACT GGTCAAATCA ATGGCGATTT GCTGATATAC
    CATGTCTTGC TGACTTTAAA GCCATATTAT GCAAAGCCAT ATGAAATTGT AGTGGACCTT
    ACGCATACTG GGCCGAGCAA TCGCTTTAAA ACAGACTTTC TCTCCAAGTG GTTTGTTGTT
    TTTCCTGGCT TCGCTTATGA CAATGTCTCT GCAGTCTATA TCTATAACTG TAACTCCTGG
    GTCAGAGAGT ACACCAAGTA TCACGAGCGG CTGCTGACTG GCCTCAAAGG CAGCAAAAGG
    CTCATCTTCA TAGACTGTCC TGGGAAACTG GCTGAGCACA TAGAGCATGA ACAACAGAAA
    CTGCCTGCTG CCACACTGGC TTTAGAAGAG GACCTGAAGG TATTCCACAA TGCTCTCAAG
    CTAGCTCACA AAGACACCAA GGTTTCCATT AAAGTTGGTT CTACTGCTGT TCAAGTAACC
    TCAGCAGAGC GAACAAAAGT CCTGGGGCAA TCGGTCTTTC TAAATGATAT TTATTATGCT
    TCGGAAATTG AAGAGATCTG CCTGGTAGAT GAGAACCAGT TTACCTTAAC CATTGCAAAC
    CAGGGCACAC CACTCACCTT CATGCACCAG GAGTGTGAAG CCATTGTTCA GTCTATCATC
    CACATCCGGA CACGCTGGGA GCTGTCACAA CCTGACTCCA TCCCCCAACA CACCAAGATT
    CGGCCAAAAG ATGTCCCTGG GACGCTGCTC AATATTGCGT TACTTAATTT AGGCAGTTCA
    GACCCTAGTT TACGGTCAGC TGCCTATAAT CTTCTGTGTG CCTTAACTTG TACCTTCAAT
    TTAAAAATCG AGGGCCAATT ACTAGAGACA TCAGGTTTAT GCATCCCTGC CAACAACACC
    CTCTTTATTG TCTCTATTAG TAAGACACTG GCAGCCAATG AGCCACATCT GACCTTAGAA
    TTTTTGGAAG AGTGTATTTC TGGATTTAGC AAGTCTAGTA TTGAATTGAA ACACCTTTGT
    TTGGAATACA TGACTCCGTG GCTGTCAAAT CTAGTCCGTT TTTGTAAGCA CAATGATGAT
    GCCAAAAGAC AGAGAGTTAC TGCTATTCTT GATAAGCTGA TAACAATGAC CATAAATGAG
    AAACAGATGT ACCCATCTAT CCAGGCCAAA ATATGGGGGA GCCTTGGGCA GATTACAGAT
    CTCCTTGATG TTGTATTAGA CAGTTTCATC AAAACCAGTG CAACAGGTGG CTTGGGATCA
    ATAAAAGCTG AGGTGATGGC AGATACTGCT GTAGCTTTGG CTTCTGGAAA CGTGAAATTG
    GTTTCAAGCA AGGTTATTGG AAGGATGTGC AAAATAATTG ACAAGACATG TTTATCTCCA
    ACTCCTACCT TAGAGCAACA TCTTATGTGG GACGATATTG CTATTCTAGC ACGCTACATG
    CTGATGCTGT CCTTCAACAA TTCCCTTGAT GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT
    GTTGTTACTT TCTTAGTAGC TACAGGGCCA CTCTCCCTTA GAGCTTCCAC ACATGGATTG
    GTCATTAATA TCATTCACTC TCTGTGTACT TGTTCACAGC TTCATTTTAG TGAAGAGACA
    AAGCAAGTTT TGAGACTCAG TTTGACAGAG TTCTCATTAC CCAAGTTTTA CCTGCTGTTT
    GGCATTAGTA AAGTCAAGTC AGCTGCCGTC ATTGCCTTTC GCTCCAGTTA CCGGGACAGG
    TCATTCTCTC CTGGCTCCTA TGAAAGGGAG ACTTTTGCTT TGACATCCTT GGAAACAGTC
    ACAGAAGCTT TGTTGGAGAT TATGGAGGCA TGTATGAGAG ATATTCCAAC ATGCAAGTGG
    CTGGACCAGT GGACAGAACT AGCTCAAAGA TTTGCATTCC AATATAATCC ATCCCTGCAA
    CCAAGAGCTC TTGTTGTCTT TGGCTGTATT AGCAAACGAG TGTCTCATGG GCAGATAAAA
    CAGATTATCC GTATTCTTAG CAAGGCACTT GAGAGTTGCT TAAAAGGACC TGATACTTAC
    AACAGTCAAG TTCTGATAGA AGCTACAGTA ATAGCACTAA CCAAATTACA ACCACTTCTT
    AATAAGGACT CCCCTCTGCA CAAAGCCCTC TTTTGGGTGG CTGTGGCTGT GCTACAGCTC
    GATGAGGTCA ACCTGTATTC AGCAGGCAGT GCGCTTCTGG AACAAAACCT GCACACCTTA
    GACAGTCTGC GGGTATTCAA TGACAAGAGT CCAGAGGAAG TATTTATGGC AATCCGGAAT
    CCTCTGGAAT GGCACTGCAA ACAAATGGAT CATTTTGTTG GACTCAATTT CAACTCTAAC
    TTTAACTTCG CACTGGTTGG ACACCTCTTA AAAGGGTACA GGCATCCTTC ACCTGCCATT
    GTGGCAAGAA CAGTCAGAAT TTTGCATACA CTACTAACTC TGGTGAACAA ACATAGAAAC
    TGTGACAAAT TTGAGGTGAA CACACAGAGC GTGGCTTATT TAGCAGCCTT ACTCACAGTG
    TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA AAACATAGAA AATCTCTTCT TCTTACTGAT
    ATTTCAATGG AAAATGTTCC TATGGATACG TATCCCATCC ATCATGGCGA CCCTAGCTAT
    AGGACGCTAA AGGAGAACCA GCCGTGGTCA TCTCCCAAAG GTTCTGAAGG GTACCTTGCA
    GCCTCCTATC CAGCCGTGGG CCAGACCAGT CCCCGAGCCA GGAAATCCAT GAGTTTGGAC
    ATGGGGCAGC CTTCTCAGGC CAATACGAAG AAGTTGCTGG GAACAAGGAA AAGTTTTGAT
    CACTTGATAT CAGACACAAA GGCTCCTAAA AGGCAAGAAA TGGAATCAGG GATCACAACA
    CCCCCCAAAA TGAGGAGAGT AGCAGAAACT GATTATGAAA TGGAAACTCA GAGGATTTCC
    TCATCACAGC AACACCCACA TTTACGCAAA GTTTCAGTGT CTGAATCAAA TGTTCTCTTG
    GATGAAGAAG TACTTACTGA TCCGAAGATC CAAGCACTTC TTCTTACTGT TCTGGCTACA
    CTGGTAAAAT ACACCACAGA TGAGTTTGAT CAGCGAATTC TTTATGAATA CTTAGCAGAA
    GCCAGTGTTG TTTTCCCCAA GGTTTTTCCT GTTGTGCACA ATTTGTTGGA CTCTAAGATC
    AACACCTTGT TGTCATTGTG CCAAGATCCA AATTTGTTAA ATCCAATCCA TGGAATCGTG
    CAGAGTGTGG TATACCATGA AGAATCCCCA CCTCAGTACC AAACATCTTA TCTGCAAAGT
    TTTGGTTTTA ATGGCTTATG GCGGTTTGCA GGACCCTTTT CAAAGCAAAC ACAAATCCCA
    GACTATGCTG AGCTTATTGT TAAGTTTCTT GATGCCTTGA TTGACACGTA TTTGCCTGGA
    ATTGATGAAG AAACCAGTGA GGAGTCCCTC CTGACACCCA CTTCTCCTTA TCCTCCTGCA
    GTGCAGAGCC AGCTTAGTAT TACTGCTAAT CTTAACCTTT CTAATTCCAT GACCTCACTT
    GCAACTTCCC AGCATTCCCC AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCAACCACT
    GGGCACTGTA ACAGTGGACG GACTCGCCAT GGATCCGCAA GCCAAGTGCA GAAGCAAAGA
    AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290776
    Clone ID Related Accession (Same CDS sequence) XM_022423327.1
    Accession Version XM_022423327.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofibromin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
    GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA
    ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA
    AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC
    AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT
    TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT
    GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT
    GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT
    CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA
    AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG
    CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT
    GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC
    GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC
    TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG
    TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC
    AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC
    CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC
    TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT
    GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT
    GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC
    CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT
    AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG
    AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA
    ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT
    ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC
    ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG
    CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC
    AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCC
    GATAGAAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCAAGT
    GGAACTACTA GTCAGATGTC CATGGATCAT GAAGAATTAC TACGTTCTTG TACTCCTGGA
    GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCAGCAGG GTGCAGCGGT
    ACTCCGCCAA TATGCCGACA AGCTCAGACT AAACTAGAAG TGGCCCTGTA CATGTTTCTG
    TGGAGCCCTG ACACTGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGTCA CCTTTGTGAG
    GAAGCAGATA TCCGGTGTGG GGTGGACGAA GTGTCGGTGC ACAACTTCTT GCCCAACTAT
    AACACATTTA TGGAATTTGC CTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT
    CAGAAAAGAG TGATGGCACT ACTAAGGCGC ATTGAGCATC CCACTGCAGG AAATACCGAG
    GCTTGGGAAG ATACACATGC GAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTACCCA
    AAAGCTAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ACAAGACCAT TGTTAAGAGG
    CGAATGTCTC ATGTCAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTACAG
    GAATGGATCA ACATGACTGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA
    AGCAATTCTG GCTTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGTAAGGGT
    TCCATGATTT CAGTGATGTC TTCAGAGGGA AATGCAGATA CACCTGTCAG CAAATTTATG
    GATCGGCTGT TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGGACC
    AATGTTAAGG ATCTGGTGGG TCTAGAATTG AGTCCTGCTC TTTATCCAAT GCTATTTAAC
    AAATTGAAGA ATACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC
    ACCAATACTC AGTTTGTGGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT
    ACTGAAGGCA GCTCTGAACA CCTGGGGCAA GCTAGCATTG AAACAATGAT GTTAAATCTA
    GTCAGATATG TTCGTGTGCT TGGGAATATG GTCCATGCAA TTCAAATAAA AACGAAACTC
    TGTCAGTTAG TCGAGGTGAT GATGGCGAGG AGAGATGACC TCTCATTTTG TCAAGAGATG
    AAATTTAGGA ATAAGATGGT AGAATACCTG ACAGACTGGG TTATGGGAAC ATCCAACCAG
    GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGACTTGG ACCAGGCAAG CATGGAAGCA
    GTAGTTTCAC TCCTGGCTGG TCTTCCACTG CAGCCGGAGG AAGGAGATGG TGTGGAATTG
    ATGGAAGCCA AGTCACAGTT ATTTCTTAAG TATTTCACAT TGTTCATGAA CCTATTGAAT
    GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAACG TGGCATGTCT
    CGGAGGCTGG CATCACTGAG GCACTGTACA GTCCTTGCAA TGTCAAACTT ACTCAATGCT
    AATGTGGACA GTGGCCTCAT GCATTCCATA GGTTTAGGTT ATCACAAAGA TCTTCAGACA
    AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA
    CTTGCGGAAA CAGTGCTGGC CGACCGGTTT GAGAGGCTGG TGGAGTTGGT GACGATGATG
    GGAGACCAGG GAGAGCTCCC AATAGCCATG GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG
    TGGGATGAAC TAGCTCGAGT TTTGGTGACT CTGTTTGATT CTCGGCATTT ACTCTACCAA
    TTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC
    CGAGGAAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC
    TACCTACAAA AACTCTTGGA TCCGTTATTA CGAATTGTGA TCACATCCTC TGATTGGCAG
    CATGTTAGCT TTGAAGTGGA TCCTACCAGA TTAGAACAGT CAGAGAGCCT TGAGGAAAAC
    CAACGGAACC TTCTTCAGAT GACTGAAAAG TTCTTCCATG CCATCATCAG TTCCTCCTCA
    GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGTGGT TAGCCAGCGT
    TTCCCTCAGA ACAGCATCGG TGCAGTGGGA AGTGCCATGT TCCTCAGATT TATCAATCCT
    GCCATTGTCT CACCGTACGA AGCAGGGATT TTAGATAAAA AGCCACCACC TAGAATTGAA
    AGGGGCTTGA AGTTAATGTC AAAGATACTT CAGAGTATTG CCAATCATGT TCTTTTCACA
    AAAGAAGAGC ATATGCGGCC TTTTAATGAT TTTGTGAAGA GCAACTTTGA TGCAGCACGG
    AGGTTTTTCC TTGATATAGC ATCTGATTGT CCTACAAGTG ATACAGTAAA TCATAGTCTT
    TCCTTCATCA GTGATGGCAA TGTGCTTGCT TTACATCGTC TACTCTGGAA CAATCAAGAG
    AAAATTGGCC AGTATCTTTC CAGCAACAGG GATCACAAAG CTGTTGGAAG ACGACCTTTC
    GATAAGATGG CAACACTTCT TGCATACCTG GGTCCTCCAG AGCACAAACC TGTGGCAGAT
    ACACACTGGT CCAGCCTTAA CCTTACCAGT TCAAAGTTTG AGGAATTTAT GACTAGGCAT
    CAAGTACATG AAAAAGAAGA GTTCAAGGCT TTGAAAACAT TAAGTATTTT CTACCAAGCA
    GGGACTTCCA AAGCTGGGAA TCCTATATTT TATTATGTTG CACGGAGGTT TAAAACTGGT
    CAAATCAATG GCGATTTGCT GATATACCAT GTCTTGCTGA CTTTAAAGCC ATATTATGCA
    AAGCCATATG AAATTGTAGT GGACCTTACG CATACTGGGC CGAGCAATCG CTTTAAAACA
    GACTTTCTCT CCAAGTGGTT TGTTGTTTTT CCTGGCTTCG CTTATGACAA TGTCTCTGCA
    GTCTATATCT ATAACTGTAA CTCCTGGGTC AGAGAGTACA CCAAGTATCA CGAGCGGCTG
    CTGACTGGCC TCAAAGGCAG CAAAAGGCTC ATCTTCATAG ACTGTCCTGG GAAACTGGCT
    GAGCACATAG AGCATGAACA ACAGAAACTG CCTGCTGCCA CACTGGCTTT AGAAGAGGAC
    CTGAAGGTAT TCCACAATGC TCTCAAGCTA GCTCACAAAG ACACCAAGGT TTCCATTAAA
    GTTGGTTCTA CTGCTGTTCA AGTAACCTCA GCAGAGCGAA CAAAAGTCCT GGGGCAATCG
    GTCTTTCTAA ATGATATTTA TTATGCTTCG GAAATTGAAG AGATCTGCCT GGTAGATGAG
    AACCAGTTTA CCTTAACCAT TGCAAACCAG GGCACACCAC TCACCTTCAT GCACCAGGAG
    TGTGAAGCCA TTGTTCAGTC TATCATCCAC ATCCGGACAC GCTGGGAGCT GTCACAACCT
    GACTCCATCC CCCAACACAC CAAGATTCGG CCAAAAGATG TCCCTGGGAC GCTGCTCAAT
    ATTGCGTTAC TTAATTTAGG CAGTTCAGAC CCTAGTTTAC GGTCAGCTGC CTATAATCTT
    CTGTGTGCCT TAACTTGTAC CTTCAATTTA AAAATCGAGG GCCAATTACT AGAGACATCA
    GGTTTATGCA TCCCTGCCAA CAACACCCTC TTTATTGTCT CTATTAGTAA GACACTGGCA
    GCCAATGAGC CACATCTGAC CTTAGAATTT TTGGAAGAGT GTATTTCTGG ATTTAGCAAG
    TCTAGTATTG AATTGAAACA CCTTTGTTTG GAATACATGA CTCCGTGGCT GTCAAATCTA
    GTCCGTTTTT GTAAGCACAA TGATGATGCC AAAAGACAGA GAGTTACTGC TATTCTTGAT
    AAGCTGATAA CAATGACCAT AAATGAGAAA CAGATGTACC CATCTATCCA GGCCAAAATA
    TGGGGGAGCC TTGGGCAGAT TACAGATCTC CTTGATGTTG TATTAGACAG TTTCATCAAA
    ACCAGTGCAA CAGGTGGCTT GGGATCAATA AAAGCTGAGG TGATGGCAGA TACTGCTGTA
    GCTTTGGCTT CTGGAAACGT GAAATTGGTT TCAAGCAAGG TTATTGGAAG GATGTGCAAA
    ATAATTGACA AGACATGTTT ATCTCCAACT CCTACCTTAG AGCAACATCT TATGTGGGAC
    GATATTGCTA TTCTAGCACG CTACATGCTG ATGCTGTCCT TCAACAATTC CCTTGATGTG
    GCAGCTCATC TCCCCTACCT CTTCCATGTT GTTACTTTCT TAGTAGCTAC AGGGCCACTC
    TCCCTTAGAG CTTCCACACA TGGATTGGTC ATTAATATCA TTCACTCTCT GTGTACTTGT
    TCACAGCTTC ATTTTAGTGA AGAGACAAAG CAAGTTTTGA GACTCAGTTT GACAGAGTTC
    TCATTACCCA AGTTTTACCT GCTGTTTGGC ATTAGTAAAG TCAAGTCAGC TGCCGTCATT
    GCCTTTCGCT CCAGTTACCG GGACAGGTCA TTCTCTCCTG GCTCCTATGA AAGGGAGACT
    TTTGCTTTGA CATCCTTGGA AACAGTCACA GAAGCTTTGT TGGAGATTAT GGAGGCATGT
    ATGAGAGATA TTCCAACATG CAAGTGGCTG GACCAGTGGA CAGAACTAGC TCAAAGATTT
    GCATTCCAAT ATAATCCATC CCTGCAACCA AGAGCTCTTG TTGTCTTTGG CTGTATTAGC
    AAACGAGTGT CTCATGGGCA GATAAAACAG ATTATCCGTA TTCTTAGCAA GGCACTTGAG
    AGTTGCTTAA AAGGACCTGA TACTTACAAC AGTCAAGTTC TGATAGAAGC TACAGTAATA
    GCACTAACCA AATTACAACC ACTTCTTAAT AAGGACTCCC CTCTGCACAA AGCCCTCTTT
    TGGGTGGCTG TGGCTGTGCT ACAGCTCGAT GAGGTCAACC TGTATTCAGC AGGCAGTGCG
    CTTCTGGAAC AAAACCTGCA CACCTTAGAC AGTCTGCGGG TATTCAATGA CAAGAGTCCA
    GAGGAAGTAT TTATGGCAAT CCGGAATCCT CTGGAATGGC ACTGCAAACA AATGGATCAT
    TTTGTTGGAC TCAATTTCAA CTCTAACTTT AACTTCGCAC TGGTTGGACA CCTCTTAAAA
    GGGTACAGGC ATCCTTCACC TGCCATTGTG GCAAGAACAG TCAGAATTTT GCATACACTA
    CTAACTCTGG TGAACAAACA TAGAAACTGT GACAAATTTG AGGTGAACAC ACAGAGCGTG
    GCTTATTTAG CAGCCTTACT CACAGTGTCT GAGGAGGTTC GAAGTCGCTG TAGCCTAAAA
    CATAGAAAAT CTCTTCTTCT TACTGATATT TCAATGGAAA ATGTTCCTAT GGATACGTAT
    CCCATCCATC ATGGCGACCC TAGCTATAGG ACGCTAAAGG AGAACCAGCC GTGGTCATCT
    CCCAAAGGTT CTGAAGGGTA CCTTGCAGCC TCCTATCCAG CCGTGGGCCA GACCAGTCCC
    CGAGCCAGGA AATCCATGAG TTTGGACATG GGGCAGCCTT CTCAGGCCAA TACGAAGAAG
    TTGCTGGGAA CAAGGAAAAG TTTTGATCAC TTGATATCAG ACACAAAGGC TCCTAAAAGG
    CAAGAAATGG AATCAGGGAT CACAACACCC CCCAAAATGA GGAGAGTAGC AGAAACTGAT
    TATGAAATGG AAACTCAGAG GATTTCCTCA TCACAGCAAC ACCCACATTT ACGCAAAGTT
    TCAGTGTCTG AATCAAATGT TCTCTTGGAT GAAGAAGTAC TTACTGATCC GAAGATCCAA
    GCACTTCTTC TTACTGTTCT GGCTACACTG GTAAAATACA CCACAGATGA GTTTGATCAG
    CGAATTCTTT ATGAATACTT AGCAGAAGCC AGTGTTGTTT TCCCCAAGGT TTTTCCTGTT
    GTGCACAATT TGTTGGACTC TAAGATCAAC ACCTTGTTGT CATTGTGCCA AGATCCAAAT
    TTGTTAAATC CAATCCATGG AATCGTGCAG AGTGTGGTAT ACCATGAAGA ATCCCCACCT
    CAGTACCAAA CATCTTATCT GCAAAGTTTT GGTTTTAATG GCTTATGGCG GTTTGCAGGA
    CCCTTTTCAA AGCAAACACA AATCCCAGAC TATGCTGAGC TTATTGTTAA GTTTCTTGAT
    GCCTTGATTG ACACGTATTT GCCTGGAATT GATGAAGAAA CCAGTGAGGA GTCCCTCCTG
    ACACCCACTT CTCCTTATCC TCCTGCAGTG CAGAGCCAGC TTAGTATTAC TGCTAATCTT
    AACCTTTCTA ATTCCATGAC CTCACTTGCA ACTTCCCAGC ATTCCCCAGG AATCGACAAG
    GAGAACGTTG AACTCTCCCC AACCACTGGG CACTGTAACA GTGGACGGAC TCGCCATGGA
    TCCGCAAGCC AAGTGCAGAA GCAAAGAAGC GCTGGCAGTT TCAAACGTAA TAGCATTAAG
    AAGATCGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279035.1
    CDS148..8439
    Translation

    Target ORF information:

    RefSeq Version XM_022423327.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423327.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
    GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA
    ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA
    AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC
    AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT
    TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT
    GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT
    GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT
    CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA
    AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG
    CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT
    GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC
    GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC
    TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG
    TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC
    AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC
    CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC
    TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT
    GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT
    GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC
    CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT
    AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG
    AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA
    ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT
    ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC
    ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG
    CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC
    AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCC
    GATAGAAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCAAGT
    GGAACTACTA GTCAGATGTC CATGGATCAT GAAGAATTAC TACGTTCTTG TACTCCTGGA
    GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCAGCAGG GTGCAGCGGT
    ACTCCGCCAA TATGCCGACA AGCTCAGACT AAACTAGAAG TGGCCCTGTA CATGTTTCTG
    TGGAGCCCTG ACACTGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGTCA CCTTTGTGAG
    GAAGCAGATA TCCGGTGTGG GGTGGACGAA GTGTCGGTGC ACAACTTCTT GCCCAACTAT
    AACACATTTA TGGAATTTGC CTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT
    CAGAAAAGAG TGATGGCACT ACTAAGGCGC ATTGAGCATC CCACTGCAGG AAATACCGAG
    GCTTGGGAAG ATACACATGC GAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTACCCA
    AAAGCTAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ACAAGACCAT TGTTAAGAGG
    CGAATGTCTC ATGTCAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTACAG
    GAATGGATCA ACATGACTGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA
    AGCAATTCTG GCTTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGTAAGGGT
    TCCATGATTT CAGTGATGTC TTCAGAGGGA AATGCAGATA CACCTGTCAG CAAATTTATG
    GATCGGCTGT TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGGACC
    AATGTTAAGG ATCTGGTGGG TCTAGAATTG AGTCCTGCTC TTTATCCAAT GCTATTTAAC
    AAATTGAAGA ATACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC
    ACCAATACTC AGTTTGTGGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT
    ACTGAAGGCA GCTCTGAACA CCTGGGGCAA GCTAGCATTG AAACAATGAT GTTAAATCTA
    GTCAGATATG TTCGTGTGCT TGGGAATATG GTCCATGCAA TTCAAATAAA AACGAAACTC
    TGTCAGTTAG TCGAGGTGAT GATGGCGAGG AGAGATGACC TCTCATTTTG TCAAGAGATG
    AAATTTAGGA ATAAGATGGT AGAATACCTG ACAGACTGGG TTATGGGAAC ATCCAACCAG
    GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGACTTGG ACCAGGCAAG CATGGAAGCA
    GTAGTTTCAC TCCTGGCTGG TCTTCCACTG CAGCCGGAGG AAGGAGATGG TGTGGAATTG
    ATGGAAGCCA AGTCACAGTT ATTTCTTAAG TATTTCACAT TGTTCATGAA CCTATTGAAT
    GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAACG TGGCATGTCT
    CGGAGGCTGG CATCACTGAG GCACTGTACA GTCCTTGCAA TGTCAAACTT ACTCAATGCT
    AATGTGGACA GTGGCCTCAT GCATTCCATA GGTTTAGGTT ATCACAAAGA TCTTCAGACA
    AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA
    CTTGCGGAAA CAGTGCTGGC CGACCGGTTT GAGAGGCTGG TGGAGTTGGT GACGATGATG
    GGAGACCAGG GAGAGCTCCC AATAGCCATG GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG
    TGGGATGAAC TAGCTCGAGT TTTGGTGACT CTGTTTGATT CTCGGCATTT ACTCTACCAA
    TTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC
    CGAGGAAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC
    TACCTACAAA AACTCTTGGA TCCGTTATTA CGAATTGTGA TCACATCCTC TGATTGGCAG
    CATGTTAGCT TTGAAGTGGA TCCTACCAGA TTAGAACAGT CAGAGAGCCT TGAGGAAAAC
    CAACGGAACC TTCTTCAGAT GACTGAAAAG TTCTTCCATG CCATCATCAG TTCCTCCTCA
    GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGTGGT TAGCCAGCGT
    TTCCCTCAGA ACAGCATCGG TGCAGTGGGA AGTGCCATGT TCCTCAGATT TATCAATCCT
    GCCATTGTCT CACCGTACGA AGCAGGGATT TTAGATAAAA AGCCACCACC TAGAATTGAA
    AGGGGCTTGA AGTTAATGTC AAAGATACTT CAGAGTATTG CCAATCATGT TCTTTTCACA
    AAAGAAGAGC ATATGCGGCC TTTTAATGAT TTTGTGAAGA GCAACTTTGA TGCAGCACGG
    AGGTTTTTCC TTGATATAGC ATCTGATTGT CCTACAAGTG ATACAGTAAA TCATAGTCTT
    TCCTTCATCA GTGATGGCAA TGTGCTTGCT TTACATCGTC TACTCTGGAA CAATCAAGAG
    AAAATTGGCC AGTATCTTTC CAGCAACAGG GATCACAAAG CTGTTGGAAG ACGACCTTTC
    GATAAGATGG CAACACTTCT TGCATACCTG GGTCCTCCAG AGCACAAACC TGTGGCAGAT
    ACACACTGGT CCAGCCTTAA CCTTACCAGT TCAAAGTTTG AGGAATTTAT GACTAGGCAT
    CAAGTACATG AAAAAGAAGA GTTCAAGGCT TTGAAAACAT TAAGTATTTT CTACCAAGCA
    GGGACTTCCA AAGCTGGGAA TCCTATATTT TATTATGTTG CACGGAGGTT TAAAACTGGT
    CAAATCAATG GCGATTTGCT GATATACCAT GTCTTGCTGA CTTTAAAGCC ATATTATGCA
    AAGCCATATG AAATTGTAGT GGACCTTACG CATACTGGGC CGAGCAATCG CTTTAAAACA
    GACTTTCTCT CCAAGTGGTT TGTTGTTTTT CCTGGCTTCG CTTATGACAA TGTCTCTGCA
    GTCTATATCT ATAACTGTAA CTCCTGGGTC AGAGAGTACA CCAAGTATCA CGAGCGGCTG
    CTGACTGGCC TCAAAGGCAG CAAAAGGCTC ATCTTCATAG ACTGTCCTGG GAAACTGGCT
    GAGCACATAG AGCATGAACA ACAGAAACTG CCTGCTGCCA CACTGGCTTT AGAAGAGGAC
    CTGAAGGTAT TCCACAATGC TCTCAAGCTA GCTCACAAAG ACACCAAGGT TTCCATTAAA
    GTTGGTTCTA CTGCTGTTCA AGTAACCTCA GCAGAGCGAA CAAAAGTCCT GGGGCAATCG
    GTCTTTCTAA ATGATATTTA TTATGCTTCG GAAATTGAAG AGATCTGCCT GGTAGATGAG
    AACCAGTTTA CCTTAACCAT TGCAAACCAG GGCACACCAC TCACCTTCAT GCACCAGGAG
    TGTGAAGCCA TTGTTCAGTC TATCATCCAC ATCCGGACAC GCTGGGAGCT GTCACAACCT
    GACTCCATCC CCCAACACAC CAAGATTCGG CCAAAAGATG TCCCTGGGAC GCTGCTCAAT
    ATTGCGTTAC TTAATTTAGG CAGTTCAGAC CCTAGTTTAC GGTCAGCTGC CTATAATCTT
    CTGTGTGCCT TAACTTGTAC CTTCAATTTA AAAATCGAGG GCCAATTACT AGAGACATCA
    GGTTTATGCA TCCCTGCCAA CAACACCCTC TTTATTGTCT CTATTAGTAA GACACTGGCA
    GCCAATGAGC CACATCTGAC CTTAGAATTT TTGGAAGAGT GTATTTCTGG ATTTAGCAAG
    TCTAGTATTG AATTGAAACA CCTTTGTTTG GAATACATGA CTCCGTGGCT GTCAAATCTA
    GTCCGTTTTT GTAAGCACAA TGATGATGCC AAAAGACAGA GAGTTACTGC TATTCTTGAT
    AAGCTGATAA CAATGACCAT AAATGAGAAA CAGATGTACC CATCTATCCA GGCCAAAATA
    TGGGGGAGCC TTGGGCAGAT TACAGATCTC CTTGATGTTG TATTAGACAG TTTCATCAAA
    ACCAGTGCAA CAGGTGGCTT GGGATCAATA AAAGCTGAGG TGATGGCAGA TACTGCTGTA
    GCTTTGGCTT CTGGAAACGT GAAATTGGTT TCAAGCAAGG TTATTGGAAG GATGTGCAAA
    ATAATTGACA AGACATGTTT ATCTCCAACT CCTACCTTAG AGCAACATCT TATGTGGGAC
    GATATTGCTA TTCTAGCACG CTACATGCTG ATGCTGTCCT TCAACAATTC CCTTGATGTG
    GCAGCTCATC TCCCCTACCT CTTCCATGTT GTTACTTTCT TAGTAGCTAC AGGGCCACTC
    TCCCTTAGAG CTTCCACACA TGGATTGGTC ATTAATATCA TTCACTCTCT GTGTACTTGT
    TCACAGCTTC ATTTTAGTGA AGAGACAAAG CAAGTTTTGA GACTCAGTTT GACAGAGTTC
    TCATTACCCA AGTTTTACCT GCTGTTTGGC ATTAGTAAAG TCAAGTCAGC TGCCGTCATT
    GCCTTTCGCT CCAGTTACCG GGACAGGTCA TTCTCTCCTG GCTCCTATGA AAGGGAGACT
    TTTGCTTTGA CATCCTTGGA AACAGTCACA GAAGCTTTGT TGGAGATTAT GGAGGCATGT
    ATGAGAGATA TTCCAACATG CAAGTGGCTG GACCAGTGGA CAGAACTAGC TCAAAGATTT
    GCATTCCAAT ATAATCCATC CCTGCAACCA AGAGCTCTTG TTGTCTTTGG CTGTATTAGC
    AAACGAGTGT CTCATGGGCA GATAAAACAG ATTATCCGTA TTCTTAGCAA GGCACTTGAG
    AGTTGCTTAA AAGGACCTGA TACTTACAAC AGTCAAGTTC TGATAGAAGC TACAGTAATA
    GCACTAACCA AATTACAACC ACTTCTTAAT AAGGACTCCC CTCTGCACAA AGCCCTCTTT
    TGGGTGGCTG TGGCTGTGCT ACAGCTCGAT GAGGTCAACC TGTATTCAGC AGGCAGTGCG
    CTTCTGGAAC AAAACCTGCA CACCTTAGAC AGTCTGCGGG TATTCAATGA CAAGAGTCCA
    GAGGAAGTAT TTATGGCAAT CCGGAATCCT CTGGAATGGC ACTGCAAACA AATGGATCAT
    TTTGTTGGAC TCAATTTCAA CTCTAACTTT AACTTCGCAC TGGTTGGACA CCTCTTAAAA
    GGGTACAGGC ATCCTTCACC TGCCATTGTG GCAAGAACAG TCAGAATTTT GCATACACTA
    CTAACTCTGG TGAACAAACA TAGAAACTGT GACAAATTTG AGGTGAACAC ACAGAGCGTG
    GCTTATTTAG CAGCCTTACT CACAGTGTCT GAGGAGGTTC GAAGTCGCTG TAGCCTAAAA
    CATAGAAAAT CTCTTCTTCT TACTGATATT TCAATGGAAA ATGTTCCTAT GGATACGTAT
    CCCATCCATC ATGGCGACCC TAGCTATAGG ACGCTAAAGG AGAACCAGCC GTGGTCATCT
    CCCAAAGGTT CTGAAGGGTA CCTTGCAGCC TCCTATCCAG CCGTGGGCCA GACCAGTCCC
    CGAGCCAGGA AATCCATGAG TTTGGACATG GGGCAGCCTT CTCAGGCCAA TACGAAGAAG
    TTGCTGGGAA CAAGGAAAAG TTTTGATCAC TTGATATCAG ACACAAAGGC TCCTAAAAGG
    CAAGAAATGG AATCAGGGAT CACAACACCC CCCAAAATGA GGAGAGTAGC AGAAACTGAT
    TATGAAATGG AAACTCAGAG GATTTCCTCA TCACAGCAAC ACCCACATTT ACGCAAAGTT
    TCAGTGTCTG AATCAAATGT TCTCTTGGAT GAAGAAGTAC TTACTGATCC GAAGATCCAA
    GCACTTCTTC TTACTGTTCT GGCTACACTG GTAAAATACA CCACAGATGA GTTTGATCAG
    CGAATTCTTT ATGAATACTT AGCAGAAGCC AGTGTTGTTT TCCCCAAGGT TTTTCCTGTT
    GTGCACAATT TGTTGGACTC TAAGATCAAC ACCTTGTTGT CATTGTGCCA AGATCCAAAT
    TTGTTAAATC CAATCCATGG AATCGTGCAG AGTGTGGTAT ACCATGAAGA ATCCCCACCT
    CAGTACCAAA CATCTTATCT GCAAAGTTTT GGTTTTAATG GCTTATGGCG GTTTGCAGGA
    CCCTTTTCAA AGCAAACACA AATCCCAGAC TATGCTGAGC TTATTGTTAA GTTTCTTGAT
    GCCTTGATTG ACACGTATTT GCCTGGAATT GATGAAGAAA CCAGTGAGGA GTCCCTCCTG
    ACACCCACTT CTCCTTATCC TCCTGCAGTG CAGAGCCAGC TTAGTATTAC TGCTAATCTT
    AACCTTTCTA ATTCCATGAC CTCACTTGCA ACTTCCCAGC ATTCCCCAGG AATCGACAAG
    GAGAACGTTG AACTCTCCCC AACCACTGGG CACTGTAACA GTGGACGGAC TCGCCATGGA
    TCCGCAAGCC AAGTGCAGAA GCAAAGAAGC GCTGGCAGTT TCAAACGTAA TAGCATTAAG
    AAGATCGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290777
    Clone ID Related Accession (Same CDS sequence) XM_022423328.1
    Accession Version XM_022423328.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8232bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofibromin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1321
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    1501
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    8221
    ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
    GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA
    ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA
    AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC
    AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT
    TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT
    GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT
    GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT
    CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA
    AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG
    CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT
    GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC
    GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC
    TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG
    TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC
    AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC
    CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC
    TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT
    GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT
    GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC
    CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT
    AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG
    AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA
    ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT
    ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC
    ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG
    CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC
    AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCC
    GATAGAAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCAAGT
    GGAACTACTA GTCAGATGTC CATGGATCAT GAAGAATTAC TACGTTCTTG TACTCCTGGA
    GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCAGCAGG GTGCAGCGGT
    ACTCCGCCAA TATGCCGACA AGCTCAGACT AAACTAGAAG TGGCCCTGTA CATGTTTCTG
    TGGAGCCCTG ACACTGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGTCA CCTTTGTGAG
    GAAGCAGATA TCCGGTGTGG GGTGGACGAA GTGTCGGTGC ACAACTTCTT GCCCAACTAT
    AACACATTTA TGGAATTTGC CTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT
    CAGAAAAGAG TGATGGCACT ACTAAGGCGC ATTGAGCATC CCACTGCAGG AAATACCGAG
    GCTTGGGAAG ATACACATGC GAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTACCCA
    AAAGCTAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ACAAGACCAT TGTTAAGAGG
    CGAATGTCTC ATGTCAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTACAG
    GAATGGATCA ACATGACTGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA
    AGCAATTCTG GCTTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGTAAGGGT
    TCCATGATTT CAGTGATGTC TTCAGAGGGA AATGCAGATA CACCTGTCAG CAAATTTATG
    GATCGGCTGT TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGGACC
    AATGTTAAGG ATCTGGTGGG TCTAGAATTG AGTCCTGCTC TTTATCCAAT GCTATTTAAC
    AAATTGAAGA ATACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC
    ACCAATACTC AGTTTGTGGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT
    ACTGAAGGCA GCTCTGAACA CCTGGGGCAA GCTAGCATTG AAACAATGAT GTTAAATCTA
    GTCAGATATG TTCGTGTGCT TGGGAATATG GTCCATGCAA TTCAAATAAA AACGAAACTC
    TGTCAGTTAG TCGAGGTGAT GATGGCGAGG AGAGATGACC TCTCATTTTG TCAAGAGATG
    AAATTTAGGA ATAAGATGGT AGAATACCTG ACAGACTGGG TTATGGGAAC ATCCAACCAG
    GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGACTTGG ACCAGGCAAG CATGGAAGCA
    GTAGTTTCAC TCCTGGCTGG TCTTCCACTG CAGCCGGAGG AAGGAGATGG TGTGGAATTG
    ATGGAAGCCA AGTCACAGTT ATTTCTTAAG TATTTCACAT TGTTCATGAA CCTATTGAAT
    GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAACG TGGCATGTCT
    CGGAGGCTGG CATCACTGAG GCACTGTACA GTCCTTGCAA TGTCAAACTT ACTCAATGCT
    AATGTGGACA GTGGCCTCAT GCATTCCATA GGTTTAGGTT ATCACAAAGA TCTTCAGACA
    AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA
    CTTGCGGAAA CAGTGCTGGC CGACCGGTTT GAGAGGCTGG TGGAGTTGGT GACGATGATG
    GGAGACCAGG GAGAGCTCCC AATAGCCATG GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG
    TGGGATGAAC TAGCTCGAGT TTTGGTGACT CTGTTTGATT CTCGGCATTT ACTCTACCAA
    TTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC
    CGAGGAAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC
    TACCTACAAA AACTCTTGGA TCCGTTATTA CGAATTGTGA TCACATCCTC TGATTGGCAG
    CATGTTAGCT TTGAAGTGGA TCCTACCAGA TTAGAACAGT CAGAGAGCCT TGAGGAAAAC
    CAACGGAACC TTCTTCAGAT GACTGAAAAG TTCTTCCATG CCATCATCAG TTCCTCCTCA
    GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGCAAC TTGCCACTCC
    CTACTGAATA AAGCTACAGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT GGTTAGCCAG
    CGTTTCCCTC AGAACAGCAT CGGTGCAGTG GGAAGTGCCA TGTTCCTCAG ATTTATCAAT
    CCTGCCATTG TCTCACCGTA CGAAGCAGGG ATTTTAGATA AAAAGCCACC ACCTAGAATT
    GAAAGGGGCT TGAAGTTAAT GTCAAAGATA CTTCAGAGTA TTGCCAATCA TGTTCTTTTC
    ACAAAAGAAG AGCATATGCG GCCTTTTAAT GATTTTGTGA AGAGCAACTT TGATGCAGCA
    CGGAGGTTTT TCCTTGATAT AGCATCTGAT TGTCCTACAA GTGATACAGT AAATCATAGT
    CTTTCCTTCA TCAGTGATGG CAATGTGCTT GCTTTACATC GTCTACTCTG GAACAATCAA
    GAGAAAATTG GCCAGTATCT TTCCAGCAAC AGGGATCACA AAGCTGTTGG AAGACGACCT
    TTCGATAAGA TGGCAACACT TCTTGCATAC CTGGGTCCTC CAGAGCACAA ACCTGTGGCA
    GATACACACT GGTCCAGCCT TAACCTTACC AGTTCAAAGT TTGAGGAATT TATGACTAGG
    CATCAAGTAC ATGAAAAAGA AGAGTTCAAG GCTTTGAAAA CATTAAGTAT TTTCTACCAA
    GCAGGGACTT CCAAAGCTGG GAATCCTATA TTTTATTATG TTGCACGGAG GTTTAAAACT
    GGTCAAATCA ATGGCGATTT GCTGATATAC CATGTCTTGC TGACTTTAAA GCCATATTAT
    GCAAAGCCAT ATGAAATTGT AGTGGACCTT ACGCATACTG GGCCGAGCAA TCGCTTTAAA
    ACAGACTTTC TCTCCAAGTG GTTTGTTGTT TTTCCTGGCT TCGCTTATGA CAATGTCTCT
    GCAGTCTATA TCTATAACTG TAACTCCTGG GTCAGAGAGT ACACCAAGTA TCACGAGCGG
    CTGCTGACTG GCCTCAAAGG CAGCAAAAGG CTCATCTTCA TAGACTGTCC TGGGAAACTG
    GCTGAGCACA TAGAGCATGA ACAACAGAAA CTGCCTGCTG CCACACTGGC TTTAGAAGAG
    GACCTGAAGG TATTCCACAA TGCTCTCAAG CTAGCTCACA AAGACACCAA GGTTTCCATT
    AAAGTTGGTT CTACTGCTGT TCAAGTAACC TCAGCAGAGC GAACAAAAGT CCTGGGGCAA
    TCGGTCTTTC TAAATGATAT TTATTATGCT TCGGAAATTG AAGAGATCTG CCTGGTAGAT
    GAGAACCAGT TTACCTTAAC CATTGCAAAC CAGGGCACAC CACTCACCTT CATGCACCAG
    GAGTGTGAAG CCATTGTTCA GTCTATCATC CACATCCGGA CACGCTGGGA GCTGTCACAA
    CCTGACTCCA TCCCCCAACA CACCAAGATT CGGCCAAAAG ATGTCCCTGG GACGCTGCTC
    AATATTGCGT TACTTAATTT AGGCAGTTCA GACCCTAGTT TACGGTCAGC TGCCTATAAT
    CTTCTGTGTG CCTTAACTTG TACCTTCAAT TTAAAAATCG AGGGCCAATT ACTAGAGACA
    TCAGGTTTAT GCATCCCTGC CAACAACACC CTCTTTATTG TCTCTATTAG TAAGACACTG
    GCAGCCAATG AGCCACATCT GACCTTAGAA TTTTTGGAAG AGTGTATTTC TGGATTTAGC
    AAGTCTAGTA TTGAATTGAA ACACCTTTGT TTGGAATACA TGACTCCGTG GCTGTCAAAT
    CTAGTCCGTT TTTGTAAGCA CAATGATGAT GCCAAAAGAC AGAGAGTTAC TGCTATTCTT
    GATAAGCTGA TAACAATGAC CATAAATGAG AAACAGATGT ACCCATCTAT CCAGGCCAAA
    ATATGGGGGA GCCTTGGGCA GATTACAGAT CTCCTTGATG TTGTATTAGA CAGTTTCATC
    AAAACCAGTG CAACAGGTGG CTTGGGATCA ATAAAAGCTG AGGTGATGGC AGATACTGCT
    GTAGCTTTGG CTTCTGGAAA CGTGAAATTG GTTTCAAGCA AGGTTATTGG AAGGATGTGC
    AAAATAATTG ACAAGACATG TTTATCTCCA ACTCCTACCT TAGAGCAACA TCTTATGTGG
    GACGATATTG CTATTCTAGC ACGCTACATG CTGATGCTGT CCTTCAACAA TTCCCTTGAT
    GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT GTTGTTACTT TCTTAGTAGC TACAGGGCCA
    CTCTCCCTTA GAGCTTCCAC ACATGGATTG GTCATTAATA TCATTCACTC TCTGTGTACT
    TGTTCACAGC TTCATTTTAG TGAAGAGACA AAGCAAGTTT TGAGACTCAG TTTGACAGAG
    TTCTCATTAC CCAAGTTTTA CCTGCTGTTT GGCATTAGTA AAGTCAAGTC AGCTGCCGTC
    ATTGCCTTTC GCTCCAGTTA CCGGGACAGG TCATTCTCTC CTGGCTCCTA TGAAAGGGAG
    ACTTTTGCTT TGACATCCTT GGAAACAGTC ACAGAAGCTT TGTTGGAGAT TATGGAGGCA
    TGTATGAGAG ATATTCCAAC ATGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAAAGA
    TTTGCATTCC AATATAATCC ATCCCTGCAA CCAAGAGCTC TTGTTGTCTT TGGCTGTATT
    AGCAAACGAG TGTCTCATGG GCAGATAAAA CAGATTATCC GTATTCTTAG CAAGGCACTT
    GAGAGTTGCT TAAAAGGACC TGATACTTAC AACAGTCAAG TTCTGATAGA AGCTACAGTA
    ATAGCACTAA CCAAATTACA ACCACTTCTT AATAAGGACT CCCCTCTGCA CAAAGCCCTC
    TTTTGGGTGG CTGTGGCTGT GCTACAGCTC GATGAGGTCA ACCTGTATTC AGCAGGCAGT
    GCGCTTCTGG AACAAAACCT GCACACCTTA GACAGTCTGC GGGTATTCAA TGACAAGAGT
    CCAGAGGAAG TATTTATGGC AATCCGGAAT CCTCTGGAAT GGCACTGCAA ACAAATGGAT
    CATTTTGTTG GACTCAATTT CAACTCTAAC TTTAACTTCG CACTGGTTGG ACACCTCTTA
    AAAGGGTACA GGCATCCTTC ACCTGCCATT GTGGCAAGAA CAGTCAGAAT TTTGCATACA
    CTACTAACTC TGGTGAACAA ACATAGAAAC TGTGACAAAT TTGAGGTGAA CACACAGAGC
    GTGGCTTATT TAGCAGCCTT ACTCACAGTG TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA
    AAACATAGAA AATCTCTTCT TCTTACTGAT ATTTCAATGG AAAATGTTCC TATGGATACG
    TATCCCATCC ATCATGGCGA CCCTAGCTAT AGGACGCTAA AGGAGAACCA GCCGTGGTCA
    TCTCCCAAAG GTTCTGAAGG GTACCTTGCA GCCTCCTATC CAGCCGTGGG CCAGACCAGT
    CCCCGAGCCA GGAAATCCAT GAGTTTGGAC ATGGGGCAGC CTTCTCAGGC CAATACGAAG
    AAGTTGCTGG AAACTCAGAG GATTTCCTCA TCACAGCAAC ACCCACATTT ACGCAAAGTT
    TCAGTGTCTG AATCAAATGT TCTCTTGGAT GAAGAAGTAC TTACTGATCC GAAGATCCAA
    GCACTTCTTC TTACTGTTCT GGCTACACTG GTAAAATACA CCACAGATGA GTTTGATCAG
    CGAATTCTTT ATGAATACTT AGCAGAAGCC AGTGTTGTTT TCCCCAAGGT TTTTCCTGTT
    GTGCACAATT TGTTGGACTC TAAGATCAAC ACCTTGTTGT CATTGTGCCA AGATCCAAAT
    TTGTTAAATC CAATCCATGG AATCGTGCAG AGTGTGGTAT ACCATGAAGA ATCCCCACCT
    CAGTACCAAA CATCTTATCT GCAAAGTTTT GGTTTTAATG GCTTATGGCG GTTTGCAGGA
    CCCTTTTCAA AGCAAACACA AATCCCAGAC TATGCTGAGC TTATTGTTAA GTTTCTTGAT
    GCCTTGATTG ACACGTATTT GCCTGGAATT GATGAAGAAA CCAGTGAGGA GTCCCTCCTG
    ACACCCACTT CTCCTTATCC TCCTGCAGTG CAGAGCCAGC TTAGTATTAC TGCTAATCTT
    AACCTTTCTA ATTCCATGAC CTCACTTGCA ACTTCCCAGC ATTCCCCAGG AATCGACAAG
    GAGAACGTTG AACTCTCCCC AACCACTGGG CACTGTAACA GTGGACGGAC TCGCCATGGA
    TCCGCAAGCC AAGTGCAGAA GCAAAGAAGC GCTGGCAGTT TCAAACGTAA TAGCATTAAG
    AAGATCGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279036.1
    CDS148..8379
    Translation

    Target ORF information:

    RefSeq Version XM_022423328.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423328.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
    GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA
    ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA
    AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC
    AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT
    TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT
    GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT
    GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT
    CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA
    AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG
    CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT
    GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC
    GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC
    TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG
    TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC
    AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC
    CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC
    TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT
    GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT
    GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC
    CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT
    AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG
    AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA
    ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT
    ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC
    ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG
    CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC
    AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCC
    GATAGAAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCAAGT
    GGAACTACTA GTCAGATGTC CATGGATCAT GAAGAATTAC TACGTTCTTG TACTCCTGGA
    GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCAGCAGG GTGCAGCGGT
    ACTCCGCCAA TATGCCGACA AGCTCAGACT AAACTAGAAG TGGCCCTGTA CATGTTTCTG
    TGGAGCCCTG ACACTGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGTCA CCTTTGTGAG
    GAAGCAGATA TCCGGTGTGG GGTGGACGAA GTGTCGGTGC ACAACTTCTT GCCCAACTAT
    AACACATTTA TGGAATTTGC CTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT
    CAGAAAAGAG TGATGGCACT ACTAAGGCGC ATTGAGCATC CCACTGCAGG AAATACCGAG
    GCTTGGGAAG ATACACATGC GAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTACCCA
    AAAGCTAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ACAAGACCAT TGTTAAGAGG
    CGAATGTCTC ATGTCAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTACAG
    GAATGGATCA ACATGACTGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA
    AGCAATTCTG GCTTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGTAAGGGT
    TCCATGATTT CAGTGATGTC TTCAGAGGGA AATGCAGATA CACCTGTCAG CAAATTTATG
    GATCGGCTGT TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGGACC
    AATGTTAAGG ATCTGGTGGG TCTAGAATTG AGTCCTGCTC TTTATCCAAT GCTATTTAAC
    AAATTGAAGA ATACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC
    ACCAATACTC AGTTTGTGGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT
    ACTGAAGGCA GCTCTGAACA CCTGGGGCAA GCTAGCATTG AAACAATGAT GTTAAATCTA
    GTCAGATATG TTCGTGTGCT TGGGAATATG GTCCATGCAA TTCAAATAAA AACGAAACTC
    TGTCAGTTAG TCGAGGTGAT GATGGCGAGG AGAGATGACC TCTCATTTTG TCAAGAGATG
    AAATTTAGGA ATAAGATGGT AGAATACCTG ACAGACTGGG TTATGGGAAC ATCCAACCAG
    GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGACTTGG ACCAGGCAAG CATGGAAGCA
    GTAGTTTCAC TCCTGGCTGG TCTTCCACTG CAGCCGGAGG AAGGAGATGG TGTGGAATTG
    ATGGAAGCCA AGTCACAGTT ATTTCTTAAG TATTTCACAT TGTTCATGAA CCTATTGAAT
    GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAACG TGGCATGTCT
    CGGAGGCTGG CATCACTGAG GCACTGTACA GTCCTTGCAA TGTCAAACTT ACTCAATGCT
    AATGTGGACA GTGGCCTCAT GCATTCCATA GGTTTAGGTT ATCACAAAGA TCTTCAGACA
    AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA
    CTTGCGGAAA CAGTGCTGGC CGACCGGTTT GAGAGGCTGG TGGAGTTGGT GACGATGATG
    GGAGACCAGG GAGAGCTCCC AATAGCCATG GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG
    TGGGATGAAC TAGCTCGAGT TTTGGTGACT CTGTTTGATT CTCGGCATTT ACTCTACCAA
    TTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC
    CGAGGAAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC
    TACCTACAAA AACTCTTGGA TCCGTTATTA CGAATTGTGA TCACATCCTC TGATTGGCAG
    CATGTTAGCT TTGAAGTGGA TCCTACCAGA TTAGAACAGT CAGAGAGCCT TGAGGAAAAC
    CAACGGAACC TTCTTCAGAT GACTGAAAAG TTCTTCCATG CCATCATCAG TTCCTCCTCA
    GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGCAAC TTGCCACTCC
    CTACTGAATA AAGCTACAGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT GGTTAGCCAG
    CGTTTCCCTC AGAACAGCAT CGGTGCAGTG GGAAGTGCCA TGTTCCTCAG ATTTATCAAT
    CCTGCCATTG TCTCACCGTA CGAAGCAGGG ATTTTAGATA AAAAGCCACC ACCTAGAATT
    GAAAGGGGCT TGAAGTTAAT GTCAAAGATA CTTCAGAGTA TTGCCAATCA TGTTCTTTTC
    ACAAAAGAAG AGCATATGCG GCCTTTTAAT GATTTTGTGA AGAGCAACTT TGATGCAGCA
    CGGAGGTTTT TCCTTGATAT AGCATCTGAT TGTCCTACAA GTGATACAGT AAATCATAGT
    CTTTCCTTCA TCAGTGATGG CAATGTGCTT GCTTTACATC GTCTACTCTG GAACAATCAA
    GAGAAAATTG GCCAGTATCT TTCCAGCAAC AGGGATCACA AAGCTGTTGG AAGACGACCT
    TTCGATAAGA TGGCAACACT TCTTGCATAC CTGGGTCCTC CAGAGCACAA ACCTGTGGCA
    GATACACACT GGTCCAGCCT TAACCTTACC AGTTCAAAGT TTGAGGAATT TATGACTAGG
    CATCAAGTAC ATGAAAAAGA AGAGTTCAAG GCTTTGAAAA CATTAAGTAT TTTCTACCAA
    GCAGGGACTT CCAAAGCTGG GAATCCTATA TTTTATTATG TTGCACGGAG GTTTAAAACT
    GGTCAAATCA ATGGCGATTT GCTGATATAC CATGTCTTGC TGACTTTAAA GCCATATTAT
    GCAAAGCCAT ATGAAATTGT AGTGGACCTT ACGCATACTG GGCCGAGCAA TCGCTTTAAA
    ACAGACTTTC TCTCCAAGTG GTTTGTTGTT TTTCCTGGCT TCGCTTATGA CAATGTCTCT
    GCAGTCTATA TCTATAACTG TAACTCCTGG GTCAGAGAGT ACACCAAGTA TCACGAGCGG
    CTGCTGACTG GCCTCAAAGG CAGCAAAAGG CTCATCTTCA TAGACTGTCC TGGGAAACTG
    GCTGAGCACA TAGAGCATGA ACAACAGAAA CTGCCTGCTG CCACACTGGC TTTAGAAGAG
    GACCTGAAGG TATTCCACAA TGCTCTCAAG CTAGCTCACA AAGACACCAA GGTTTCCATT
    AAAGTTGGTT CTACTGCTGT TCAAGTAACC TCAGCAGAGC GAACAAAAGT CCTGGGGCAA
    TCGGTCTTTC TAAATGATAT TTATTATGCT TCGGAAATTG AAGAGATCTG CCTGGTAGAT
    GAGAACCAGT TTACCTTAAC CATTGCAAAC CAGGGCACAC CACTCACCTT CATGCACCAG
    GAGTGTGAAG CCATTGTTCA GTCTATCATC CACATCCGGA CACGCTGGGA GCTGTCACAA
    CCTGACTCCA TCCCCCAACA CACCAAGATT CGGCCAAAAG ATGTCCCTGG GACGCTGCTC
    AATATTGCGT TACTTAATTT AGGCAGTTCA GACCCTAGTT TACGGTCAGC TGCCTATAAT
    CTTCTGTGTG CCTTAACTTG TACCTTCAAT TTAAAAATCG AGGGCCAATT ACTAGAGACA
    TCAGGTTTAT GCATCCCTGC CAACAACACC CTCTTTATTG TCTCTATTAG TAAGACACTG
    GCAGCCAATG AGCCACATCT GACCTTAGAA TTTTTGGAAG AGTGTATTTC TGGATTTAGC
    AAGTCTAGTA TTGAATTGAA ACACCTTTGT TTGGAATACA TGACTCCGTG GCTGTCAAAT
    CTAGTCCGTT TTTGTAAGCA CAATGATGAT GCCAAAAGAC AGAGAGTTAC TGCTATTCTT
    GATAAGCTGA TAACAATGAC CATAAATGAG AAACAGATGT ACCCATCTAT CCAGGCCAAA
    ATATGGGGGA GCCTTGGGCA GATTACAGAT CTCCTTGATG TTGTATTAGA CAGTTTCATC
    AAAACCAGTG CAACAGGTGG CTTGGGATCA ATAAAAGCTG AGGTGATGGC AGATACTGCT
    GTAGCTTTGG CTTCTGGAAA CGTGAAATTG GTTTCAAGCA AGGTTATTGG AAGGATGTGC
    AAAATAATTG ACAAGACATG TTTATCTCCA ACTCCTACCT TAGAGCAACA TCTTATGTGG
    GACGATATTG CTATTCTAGC ACGCTACATG CTGATGCTGT CCTTCAACAA TTCCCTTGAT
    GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT GTTGTTACTT TCTTAGTAGC TACAGGGCCA
    CTCTCCCTTA GAGCTTCCAC ACATGGATTG GTCATTAATA TCATTCACTC TCTGTGTACT
    TGTTCACAGC TTCATTTTAG TGAAGAGACA AAGCAAGTTT TGAGACTCAG TTTGACAGAG
    TTCTCATTAC CCAAGTTTTA CCTGCTGTTT GGCATTAGTA AAGTCAAGTC AGCTGCCGTC
    ATTGCCTTTC GCTCCAGTTA CCGGGACAGG TCATTCTCTC CTGGCTCCTA TGAAAGGGAG
    ACTTTTGCTT TGACATCCTT GGAAACAGTC ACAGAAGCTT TGTTGGAGAT TATGGAGGCA
    TGTATGAGAG ATATTCCAAC ATGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAAAGA
    TTTGCATTCC AATATAATCC ATCCCTGCAA CCAAGAGCTC TTGTTGTCTT TGGCTGTATT
    AGCAAACGAG TGTCTCATGG GCAGATAAAA CAGATTATCC GTATTCTTAG CAAGGCACTT
    GAGAGTTGCT TAAAAGGACC TGATACTTAC AACAGTCAAG TTCTGATAGA AGCTACAGTA
    ATAGCACTAA CCAAATTACA ACCACTTCTT AATAAGGACT CCCCTCTGCA CAAAGCCCTC
    TTTTGGGTGG CTGTGGCTGT GCTACAGCTC GATGAGGTCA ACCTGTATTC AGCAGGCAGT
    GCGCTTCTGG AACAAAACCT GCACACCTTA GACAGTCTGC GGGTATTCAA TGACAAGAGT
    CCAGAGGAAG TATTTATGGC AATCCGGAAT CCTCTGGAAT GGCACTGCAA ACAAATGGAT
    CATTTTGTTG GACTCAATTT CAACTCTAAC TTTAACTTCG CACTGGTTGG ACACCTCTTA
    AAAGGGTACA GGCATCCTTC ACCTGCCATT GTGGCAAGAA CAGTCAGAAT TTTGCATACA
    CTACTAACTC TGGTGAACAA ACATAGAAAC TGTGACAAAT TTGAGGTGAA CACACAGAGC
    GTGGCTTATT TAGCAGCCTT ACTCACAGTG TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA
    AAACATAGAA AATCTCTTCT TCTTACTGAT ATTTCAATGG AAAATGTTCC TATGGATACG
    TATCCCATCC ATCATGGCGA CCCTAGCTAT AGGACGCTAA AGGAGAACCA GCCGTGGTCA
    TCTCCCAAAG GTTCTGAAGG GTACCTTGCA GCCTCCTATC CAGCCGTGGG CCAGACCAGT
    CCCCGAGCCA GGAAATCCAT GAGTTTGGAC ATGGGGCAGC CTTCTCAGGC CAATACGAAG
    AAGTTGCTGG AAACTCAGAG GATTTCCTCA TCACAGCAAC ACCCACATTT ACGCAAAGTT
    TCAGTGTCTG AATCAAATGT TCTCTTGGAT GAAGAAGTAC TTACTGATCC GAAGATCCAA
    GCACTTCTTC TTACTGTTCT GGCTACACTG GTAAAATACA CCACAGATGA GTTTGATCAG
    CGAATTCTTT ATGAATACTT AGCAGAAGCC AGTGTTGTTT TCCCCAAGGT TTTTCCTGTT
    GTGCACAATT TGTTGGACTC TAAGATCAAC ACCTTGTTGT CATTGTGCCA AGATCCAAAT
    TTGTTAAATC CAATCCATGG AATCGTGCAG AGTGTGGTAT ACCATGAAGA ATCCCCACCT
    CAGTACCAAA CATCTTATCT GCAAAGTTTT GGTTTTAATG GCTTATGGCG GTTTGCAGGA
    CCCTTTTCAA AGCAAACACA AATCCCAGAC TATGCTGAGC TTATTGTTAA GTTTCTTGAT
    GCCTTGATTG ACACGTATTT GCCTGGAATT GATGAAGAAA CCAGTGAGGA GTCCCTCCTG
    ACACCCACTT CTCCTTATCC TCCTGCAGTG CAGAGCCAGC TTAGTATTAC TGCTAATCTT
    AACCTTTCTA ATTCCATGAC CTCACTTGCA ACTTCCCAGC ATTCCCCAGG AATCGACAAG
    GAGAACGTTG AACTCTCCCC AACCACTGGG CACTGTAACA GTGGACGGAC TCGCCATGGA
    TCCGCAAGCC AAGTGCAGAA GCAAAGAAGC GCTGGCAGTT TCAAACGTAA TAGCATTAAG
    AAGATCGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290778
    Clone ID Related Accession (Same CDS sequence) XM_022423329.1
    Accession Version XM_022423329.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8172bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofibromin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
    GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA
    ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA
    AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC
    AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT
    TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT
    GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT
    GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT
    CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA
    AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG
    CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT
    GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC
    GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC
    TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG
    TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC
    AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC
    CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC
    TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT
    GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT
    GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC
    CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT
    AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG
    AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA
    ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT
    ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC
    ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG
    CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC
    AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCC
    GATAGAAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCAAGT
    GGAACTACTA GTCAGATGTC CATGGATCAT GAAGAATTAC TACGTTCTTG TACTCCTGGA
    GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCAGCAGG GTGCAGCGGT
    ACTCCGCCAA TATGCCGACA AGCTCAGACT AAACTAGAAG TGGCCCTGTA CATGTTTCTG
    TGGAGCCCTG ACACTGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGTCA CCTTTGTGAG
    GAAGCAGATA TCCGGTGTGG GGTGGACGAA GTGTCGGTGC ACAACTTCTT GCCCAACTAT
    AACACATTTA TGGAATTTGC CTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT
    CAGAAAAGAG TGATGGCACT ACTAAGGCGC ATTGAGCATC CCACTGCAGG AAATACCGAG
    GCTTGGGAAG ATACACATGC GAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTACCCA
    AAAGCTAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ACAAGACCAT TGTTAAGAGG
    CGAATGTCTC ATGTCAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTACAG
    GAATGGATCA ACATGACTGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA
    AGCAATTCTG GCTTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGTAAGGGT
    TCCATGATTT CAGTGATGTC TTCAGAGGGA AATGCAGATA CACCTGTCAG CAAATTTATG
    GATCGGCTGT TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGGACC
    AATGTTAAGG ATCTGGTGGG TCTAGAATTG AGTCCTGCTC TTTATCCAAT GCTATTTAAC
    AAATTGAAGA ATACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC
    ACCAATACTC AGTTTGTGGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT
    ACTGAAGGCA GCTCTGAACA CCTGGGGCAA GCTAGCATTG AAACAATGAT GTTAAATCTA
    GTCAGATATG TTCGTGTGCT TGGGAATATG GTCCATGCAA TTCAAATAAA AACGAAACTC
    TGTCAGTTAG TCGAGGTGAT GATGGCGAGG AGAGATGACC TCTCATTTTG TCAAGAGATG
    AAATTTAGGA ATAAGATGGT AGAATACCTG ACAGACTGGG TTATGGGAAC ATCCAACCAG
    GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGACTTGG ACCAGGCAAG CATGGAAGCA
    GTAGTTTCAC TCCTGGCTGG TCTTCCACTG CAGCCGGAGG AAGGAGATGG TGTGGAATTG
    ATGGAAGCCA AGTCACAGTT ATTTCTTAAG TATTTCACAT TGTTCATGAA CCTATTGAAT
    GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAACG TGGCATGTCT
    CGGAGGCTGG CATCACTGAG GCACTGTACA GTCCTTGCAA TGTCAAACTT ACTCAATGCT
    AATGTGGACA GTGGCCTCAT GCATTCCATA GGTTTAGGTT ATCACAAAGA TCTTCAGACA
    AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA
    CTTGCGGAAA CAGTGCTGGC CGACCGGTTT GAGAGGCTGG TGGAGTTGGT GACGATGATG
    GGAGACCAGG GAGAGCTCCC AATAGCCATG GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG
    TGGGATGAAC TAGCTCGAGT TTTGGTGACT CTGTTTGATT CTCGGCATTT ACTCTACCAA
    TTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC
    CGAGGAAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC
    TACCTACAAA AACTCTTGGA TCCGTTATTA CGAATTGTGA TCACATCCTC TGATTGGCAG
    CATGTTAGCT TTGAAGTGGA TCCTACCAGA TTAGAACAGT CAGAGAGCCT TGAGGAAAAC
    CAACGGAACC TTCTTCAGAT GACTGAAAAG TTCTTCCATG CCATCATCAG TTCCTCCTCA
    GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGCAAC TTGCCACTCC
    CTACTGAATA AAGCTACAGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT GGTTAGCCAG
    CGTTTCCCTC AGAACAGCAT CGGTGCAGTG GGAAGTGCCA TGTTCCTCAG ATTTATCAAT
    CCTGCCATTG TCTCACCGTA CGAAGCAGGG ATTTTAGATA AAAAGCCACC ACCTAGAATT
    GAAAGGGGCT TGAAGTTAAT GTCAAAGATA CTTCAGAGTA TTGCCAATCA TGTTCTTTTC
    ACAAAAGAAG AGCATATGCG GCCTTTTAAT GATTTTGTGA AGAGCAACTT TGATGCAGCA
    CGGAGGTTTT TCCTTGATAT AGCATCTGAT TGTCCTACAA GTGATACAGT AAATCATAGT
    CTTTCCTTCA TCAGTGATGG CAATGTGCTT GCTTTACATC GTCTACTCTG GAACAATCAA
    GAGAAAATTG GCCAGTATCT TTCCAGCAAC AGGGATCACA AAGCTGTTGG AAGACGACCT
    TTCGATAAGA TGGCAACACT TCTTGCATAC CTGGGTCCTC CAGAGCACAA ACCTGTGGCA
    GATACACACT GGTCCAGCCT TAACCTTACC AGTTCAAAGT TTGAGGAATT TATGACTAGG
    CATCAAGTAC ATGAAAAAGA AGAGTTCAAG GCTTTGAAAA CATTAAGTAT TTTCTACCAA
    GCAGGGACTT CCAAAGCTGG GAATCCTATA TTTTATTATG TTGCACGGAG GTTTAAAACT
    GGTCAAATCA ATGGCGATTT GCTGATATAC CATGTCTTGC TGACTTTAAA GCCATATTAT
    GCAAAGCCAT ATGAAATTGT AGTGGACCTT ACGCATACTG GGCCGAGCAA TCGCTTTAAA
    ACAGACTTTC TCTCCAAGTG GTTTGTTGTT TTTCCTGGCT TCGCTTATGA CAATGTCTCT
    GCAGTCTATA TCTATAACTG TAACTCCTGG GTCAGAGAGT ACACCAAGTA TCACGAGCGG
    CTGCTGACTG GCCTCAAAGG CAGCAAAAGG CTCATCTTCA TAGACTGTCC TGGGAAACTG
    GCTGAGCACA TAGAGCATGA ACAACAGAAA CTGCCTGCTG CCACACTGGC TTTAGAAGAG
    GACCTGAAGG TATTCCACAA TGCTCTCAAG CTAGCTCACA AAGACACCAA GGTTTCCATT
    AAAGTTGGTT CTACTGCTGT TCAAGTAACC TCAGCAGAGC GAACAAAAGT CCTGGGGCAA
    TCGGTCTTTC TAAATGATAT TTATTATGCT TCGGAAATTG AAGAGATCTG CCTGGTAGAT
    GAGAACCAGT TTACCTTAAC CATTGCAAAC CAGGGCACAC CACTCACCTT CATGCACCAG
    GAGTGTGAAG CCATTGTTCA GTCTATCATC CACATCCGGA CACGCTGGGA GCTGTCACAA
    CCTGACTCCA TCCCCCAACA CACCAAGATT CGGCCAAAAG ATGTCCCTGG GACGCTGCTC
    AATATTGCGT TACTTAATTT AGGCAGTTCA GACCCTAGTT TACGGTCAGC TGCCTATAAT
    CTTCTGTGTG CCTTAACTTG TACCTTCAAT TTAAAAATCG AGGGCCAATT ACTAGAGACA
    TCAGGTTTAT GCATCCCTGC CAACAACACC CTCTTTATTG TCTCTATTAG TAAGACACTG
    GCAGCCAATG AGCCACATCT GACCTTAGAA TTTTTGGAAG AGTGTATTTC TGGATTTAGC
    AAGTCTAGTA TTGAATTGAA ACACCTTTGT TTGGAATACA TGACTCCGTG GCTGTCAAAT
    CTAGTCCGTT TTTGTAAGCA CAATGATGAT GCCAAAAGAC AGAGAGTTAC TGCTATTCTT
    GATAAGCTGA TAACAATGAC CATAAATGAG AAACAGATGT ACCCATCTAT CCAGGCCAAA
    ATATGGGGGA GCCTTGGGCA GATTACAGAT CTCCTTGATG TTGTATTAGA CAGTTTCATC
    AAAACCAGTG CAACAGGTGG CTTGGGATCA ATAAAAGCTG AGGTGATGGC AGATACTGCT
    GTAGCTTTGG CTTCTGGAAA CGTGAAATTG GTTTCAAGCA AGGTTATTGG AAGGATGTGC
    AAAATAATTG ACAAGACATG TTTATCTCCA ACTCCTACCT TAGAGCAACA TCTTATGTGG
    GACGATATTG CTATTCTAGC ACGCTACATG CTGATGCTGT CCTTCAACAA TTCCCTTGAT
    GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT GTTGTTACTT TCTTAGTAGC TACAGGGCCA
    CTCTCCCTTA GAGCTTCCAC ACATGGATTG GTCATTAATA TCATTCACTC TCTGTGTACT
    TGTTCACAGC TTCATTTTAG TGAAGAGACA AAGCAAGTTT TGAGACTCAG TTTGACAGAG
    TTCTCATTAC CCAAGTTTTA CCTGCTGTTT GGCATTAGTA AAGTCAAGTC AGCTGCCGTC
    ATTGCCTTTC GCTCCAGTTA CCGGGACAGG TCATTCTCTC CTGGCTCCTA TGAAAGGGAG
    ACTTTTGCTT TGACATCCTT GGAAACAGTC ACAGAAGCTT TGTTGGAGAT TATGGAGGCA
    TGTATGAGAG ATATTCCAAC ATGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAAAGA
    TTTGCATTCC AATATAATCC ATCCCTGCAA CCAAGAGCTC TTGTTGTCTT TGGCTGTATT
    AGCAAACGAG TGTCTCATGG GCAGATAAAA CAGATTATCC GTATTCTTAG CAAGGCACTT
    GAGAGTTGCT TAAAAGGACC TGATACTTAC AACAGTCAAG TTCTGATAGA AGCTACAGTA
    ATAGCACTAA CCAAATTACA ACCACTTCTT AATAAGGACT CCCCTCTGCA CAAAGCCCTC
    TTTTGGGTGG CTGTGGCTGT GCTACAGCTC GATGAGGTCA ACCTGTATTC AGCAGGCAGT
    GCGCTTCTGG AACAAAACCT GCACACCTTA GACAGTCTGC GGGTATTCAA TGACAAGAGT
    CCAGAGGAAG TATTTATGGC AATCCGGAAT CCTCTGGAAT GGCACTGCAA ACAAATGGAT
    CATTTTGTTG GACTCAATTT CAACTCTAAC TTTAACTTCG CACTGGTTGG ACACCTCTTA
    AAAGGGTACA GGCATCCTTC ACCTGCCATT GTGGCAAGAA CAGTCAGAAT TTTGCATACA
    CTACTAACTC TGGTGAACAA ACATAGAAAC TGTGACAAAT TTGAGGTGAA CACACAGAGC
    GTGGCTTATT TAGCAGCCTT ACTCACAGTG TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA
    AAACATAGAA AATCTCTTCT TCTTACTGAT ATTTCAATGG AAAATGTTCC TATGGATACG
    TATCCCATCC ATCATGGCGA CCCTAGCTAT AGGACGCTAA AGGAGAACCA GCCGTGGTCA
    TCTCCCAAAG GTTCTGAAGG GTACCTTGCA GCCTCCTATC CAGCCGTGGG CCAGACCAGT
    CCCCGAGCCA GGAAATCCAT GAGTTTGGAC ATGGGGCAGC CTTCTCAGGC CAATACGAAG
    AAGTTGCTGG GAACAAGGAA AAGTTTTGAT CACTTGATAT CAGACACAAA GGCTCCTAAA
    AGGCAAGAAA TGGAATCAGG GATCACAACA CCCCCCAAAA TGAGGAGAGT AGCAGAAACT
    GATTATGAAA TGGAAACTCA GAGGATTTCC TCATCACAGC AACACCCACA TTTACGCAAA
    GTTTCAGTGT CTGAATCAAA TGTTCTCTTG GATGAAGAAG TACTTACTGA TCCGAAGATC
    CAAGCACTTC TTCTTACTGT TCTGGCTACA CTGGTAAAAT ACACCACAGA TGAGTTTGAT
    CAGCGAATTC TTTATGAATA CTTAGCAGAA GCCAGTGTTG TTTTCCCCAA GGTTTTTCCT
    GTTGTGCACA ATTTGTTGGA CTCTAAGATC AACACCTTGT TGTCATTGTG CCAAGATCCA
    AATTTGTTAA ATCCAATCCA TGGAATCGTG CAGAGTGTGG TATACCATGA AGAATCCCCA
    CCTCAGTACC AAACATCTTA TCTGCAAAGT TTTGGTTTTA ATGGCTTATG GCGGTTTGCA
    GGACCCTTTT CAAAGGAATC GACAAGGAGA ACGTTGAACT CTCCCCAACC ACTGGGCACT
    GTAACAGTGG ACGGACTCGC CATGGATCCG CAAGCCAAGT GCAGAAGCAA AGAAGCGCTG
    GCAGTTTCAA ACGTAATAGC ATTAAGAAGA TCGTGTGAAA CTTTCTTTCT TTCCTTTTTC
    AAACAGACTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279037.1
    CDS148..8319
    Translation

    Target ORF information:

    RefSeq Version XM_022423329.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423329.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
    GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA
    ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA
    AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC
    AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT
    TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT
    GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT
    GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT
    CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA
    AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG
    CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT
    GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC
    GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC
    TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG
    TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC
    AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC
    CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC
    TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT
    GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT
    GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC
    CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT
    AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG
    AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA
    ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT
    ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC
    ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG
    CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC
    AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCC
    GATAGAAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCAAGT
    GGAACTACTA GTCAGATGTC CATGGATCAT GAAGAATTAC TACGTTCTTG TACTCCTGGA
    GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCAGCAGG GTGCAGCGGT
    ACTCCGCCAA TATGCCGACA AGCTCAGACT AAACTAGAAG TGGCCCTGTA CATGTTTCTG
    TGGAGCCCTG ACACTGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGTCA CCTTTGTGAG
    GAAGCAGATA TCCGGTGTGG GGTGGACGAA GTGTCGGTGC ACAACTTCTT GCCCAACTAT
    AACACATTTA TGGAATTTGC CTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT
    CAGAAAAGAG TGATGGCACT ACTAAGGCGC ATTGAGCATC CCACTGCAGG AAATACCGAG
    GCTTGGGAAG ATACACATGC GAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTACCCA
    AAAGCTAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ACAAGACCAT TGTTAAGAGG
    CGAATGTCTC ATGTCAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTACAG
    GAATGGATCA ACATGACTGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA
    AGCAATTCTG GCTTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGTAAGGGT
    TCCATGATTT CAGTGATGTC TTCAGAGGGA AATGCAGATA CACCTGTCAG CAAATTTATG
    GATCGGCTGT TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGGACC
    AATGTTAAGG ATCTGGTGGG TCTAGAATTG AGTCCTGCTC TTTATCCAAT GCTATTTAAC
    AAATTGAAGA ATACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC
    ACCAATACTC AGTTTGTGGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT
    ACTGAAGGCA GCTCTGAACA CCTGGGGCAA GCTAGCATTG AAACAATGAT GTTAAATCTA
    GTCAGATATG TTCGTGTGCT TGGGAATATG GTCCATGCAA TTCAAATAAA AACGAAACTC
    TGTCAGTTAG TCGAGGTGAT GATGGCGAGG AGAGATGACC TCTCATTTTG TCAAGAGATG
    AAATTTAGGA ATAAGATGGT AGAATACCTG ACAGACTGGG TTATGGGAAC ATCCAACCAG
    GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGACTTGG ACCAGGCAAG CATGGAAGCA
    GTAGTTTCAC TCCTGGCTGG TCTTCCACTG CAGCCGGAGG AAGGAGATGG TGTGGAATTG
    ATGGAAGCCA AGTCACAGTT ATTTCTTAAG TATTTCACAT TGTTCATGAA CCTATTGAAT
    GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAACG TGGCATGTCT
    CGGAGGCTGG CATCACTGAG GCACTGTACA GTCCTTGCAA TGTCAAACTT ACTCAATGCT
    AATGTGGACA GTGGCCTCAT GCATTCCATA GGTTTAGGTT ATCACAAAGA TCTTCAGACA
    AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA
    CTTGCGGAAA CAGTGCTGGC CGACCGGTTT GAGAGGCTGG TGGAGTTGGT GACGATGATG
    GGAGACCAGG GAGAGCTCCC AATAGCCATG GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG
    TGGGATGAAC TAGCTCGAGT TTTGGTGACT CTGTTTGATT CTCGGCATTT ACTCTACCAA
    TTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC
    CGAGGAAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC
    TACCTACAAA AACTCTTGGA TCCGTTATTA CGAATTGTGA TCACATCCTC TGATTGGCAG
    CATGTTAGCT TTGAAGTGGA TCCTACCAGA TTAGAACAGT CAGAGAGCCT TGAGGAAAAC
    CAACGGAACC TTCTTCAGAT GACTGAAAAG TTCTTCCATG CCATCATCAG TTCCTCCTCA
    GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGCAAC TTGCCACTCC
    CTACTGAATA AAGCTACAGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT GGTTAGCCAG
    CGTTTCCCTC AGAACAGCAT CGGTGCAGTG GGAAGTGCCA TGTTCCTCAG ATTTATCAAT
    CCTGCCATTG TCTCACCGTA CGAAGCAGGG ATTTTAGATA AAAAGCCACC ACCTAGAATT
    GAAAGGGGCT TGAAGTTAAT GTCAAAGATA CTTCAGAGTA TTGCCAATCA TGTTCTTTTC
    ACAAAAGAAG AGCATATGCG GCCTTTTAAT GATTTTGTGA AGAGCAACTT TGATGCAGCA
    CGGAGGTTTT TCCTTGATAT AGCATCTGAT TGTCCTACAA GTGATACAGT AAATCATAGT
    CTTTCCTTCA TCAGTGATGG CAATGTGCTT GCTTTACATC GTCTACTCTG GAACAATCAA
    GAGAAAATTG GCCAGTATCT TTCCAGCAAC AGGGATCACA AAGCTGTTGG AAGACGACCT
    TTCGATAAGA TGGCAACACT TCTTGCATAC CTGGGTCCTC CAGAGCACAA ACCTGTGGCA
    GATACACACT GGTCCAGCCT TAACCTTACC AGTTCAAAGT TTGAGGAATT TATGACTAGG
    CATCAAGTAC ATGAAAAAGA AGAGTTCAAG GCTTTGAAAA CATTAAGTAT TTTCTACCAA
    GCAGGGACTT CCAAAGCTGG GAATCCTATA TTTTATTATG TTGCACGGAG GTTTAAAACT
    GGTCAAATCA ATGGCGATTT GCTGATATAC CATGTCTTGC TGACTTTAAA GCCATATTAT
    GCAAAGCCAT ATGAAATTGT AGTGGACCTT ACGCATACTG GGCCGAGCAA TCGCTTTAAA
    ACAGACTTTC TCTCCAAGTG GTTTGTTGTT TTTCCTGGCT TCGCTTATGA CAATGTCTCT
    GCAGTCTATA TCTATAACTG TAACTCCTGG GTCAGAGAGT ACACCAAGTA TCACGAGCGG
    CTGCTGACTG GCCTCAAAGG CAGCAAAAGG CTCATCTTCA TAGACTGTCC TGGGAAACTG
    GCTGAGCACA TAGAGCATGA ACAACAGAAA CTGCCTGCTG CCACACTGGC TTTAGAAGAG
    GACCTGAAGG TATTCCACAA TGCTCTCAAG CTAGCTCACA AAGACACCAA GGTTTCCATT
    AAAGTTGGTT CTACTGCTGT TCAAGTAACC TCAGCAGAGC GAACAAAAGT CCTGGGGCAA
    TCGGTCTTTC TAAATGATAT TTATTATGCT TCGGAAATTG AAGAGATCTG CCTGGTAGAT
    GAGAACCAGT TTACCTTAAC CATTGCAAAC CAGGGCACAC CACTCACCTT CATGCACCAG
    GAGTGTGAAG CCATTGTTCA GTCTATCATC CACATCCGGA CACGCTGGGA GCTGTCACAA
    CCTGACTCCA TCCCCCAACA CACCAAGATT CGGCCAAAAG ATGTCCCTGG GACGCTGCTC
    AATATTGCGT TACTTAATTT AGGCAGTTCA GACCCTAGTT TACGGTCAGC TGCCTATAAT
    CTTCTGTGTG CCTTAACTTG TACCTTCAAT TTAAAAATCG AGGGCCAATT ACTAGAGACA
    TCAGGTTTAT GCATCCCTGC CAACAACACC CTCTTTATTG TCTCTATTAG TAAGACACTG
    GCAGCCAATG AGCCACATCT GACCTTAGAA TTTTTGGAAG AGTGTATTTC TGGATTTAGC
    AAGTCTAGTA TTGAATTGAA ACACCTTTGT TTGGAATACA TGACTCCGTG GCTGTCAAAT
    CTAGTCCGTT TTTGTAAGCA CAATGATGAT GCCAAAAGAC AGAGAGTTAC TGCTATTCTT
    GATAAGCTGA TAACAATGAC CATAAATGAG AAACAGATGT ACCCATCTAT CCAGGCCAAA
    ATATGGGGGA GCCTTGGGCA GATTACAGAT CTCCTTGATG TTGTATTAGA CAGTTTCATC
    AAAACCAGTG CAACAGGTGG CTTGGGATCA ATAAAAGCTG AGGTGATGGC AGATACTGCT
    GTAGCTTTGG CTTCTGGAAA CGTGAAATTG GTTTCAAGCA AGGTTATTGG AAGGATGTGC
    AAAATAATTG ACAAGACATG TTTATCTCCA ACTCCTACCT TAGAGCAACA TCTTATGTGG
    GACGATATTG CTATTCTAGC ACGCTACATG CTGATGCTGT CCTTCAACAA TTCCCTTGAT
    GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT GTTGTTACTT TCTTAGTAGC TACAGGGCCA
    CTCTCCCTTA GAGCTTCCAC ACATGGATTG GTCATTAATA TCATTCACTC TCTGTGTACT
    TGTTCACAGC TTCATTTTAG TGAAGAGACA AAGCAAGTTT TGAGACTCAG TTTGACAGAG
    TTCTCATTAC CCAAGTTTTA CCTGCTGTTT GGCATTAGTA AAGTCAAGTC AGCTGCCGTC
    ATTGCCTTTC GCTCCAGTTA CCGGGACAGG TCATTCTCTC CTGGCTCCTA TGAAAGGGAG
    ACTTTTGCTT TGACATCCTT GGAAACAGTC ACAGAAGCTT TGTTGGAGAT TATGGAGGCA
    TGTATGAGAG ATATTCCAAC ATGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAAAGA
    TTTGCATTCC AATATAATCC ATCCCTGCAA CCAAGAGCTC TTGTTGTCTT TGGCTGTATT
    AGCAAACGAG TGTCTCATGG GCAGATAAAA CAGATTATCC GTATTCTTAG CAAGGCACTT
    GAGAGTTGCT TAAAAGGACC TGATACTTAC AACAGTCAAG TTCTGATAGA AGCTACAGTA
    ATAGCACTAA CCAAATTACA ACCACTTCTT AATAAGGACT CCCCTCTGCA CAAAGCCCTC
    TTTTGGGTGG CTGTGGCTGT GCTACAGCTC GATGAGGTCA ACCTGTATTC AGCAGGCAGT
    GCGCTTCTGG AACAAAACCT GCACACCTTA GACAGTCTGC GGGTATTCAA TGACAAGAGT
    CCAGAGGAAG TATTTATGGC AATCCGGAAT CCTCTGGAAT GGCACTGCAA ACAAATGGAT
    CATTTTGTTG GACTCAATTT CAACTCTAAC TTTAACTTCG CACTGGTTGG ACACCTCTTA
    AAAGGGTACA GGCATCCTTC ACCTGCCATT GTGGCAAGAA CAGTCAGAAT TTTGCATACA
    CTACTAACTC TGGTGAACAA ACATAGAAAC TGTGACAAAT TTGAGGTGAA CACACAGAGC
    GTGGCTTATT TAGCAGCCTT ACTCACAGTG TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA
    AAACATAGAA AATCTCTTCT TCTTACTGAT ATTTCAATGG AAAATGTTCC TATGGATACG
    TATCCCATCC ATCATGGCGA CCCTAGCTAT AGGACGCTAA AGGAGAACCA GCCGTGGTCA
    TCTCCCAAAG GTTCTGAAGG GTACCTTGCA GCCTCCTATC CAGCCGTGGG CCAGACCAGT
    CCCCGAGCCA GGAAATCCAT GAGTTTGGAC ATGGGGCAGC CTTCTCAGGC CAATACGAAG
    AAGTTGCTGG GAACAAGGAA AAGTTTTGAT CACTTGATAT CAGACACAAA GGCTCCTAAA
    AGGCAAGAAA TGGAATCAGG GATCACAACA CCCCCCAAAA TGAGGAGAGT AGCAGAAACT
    GATTATGAAA TGGAAACTCA GAGGATTTCC TCATCACAGC AACACCCACA TTTACGCAAA
    GTTTCAGTGT CTGAATCAAA TGTTCTCTTG GATGAAGAAG TACTTACTGA TCCGAAGATC
    CAAGCACTTC TTCTTACTGT TCTGGCTACA CTGGTAAAAT ACACCACAGA TGAGTTTGAT
    CAGCGAATTC TTTATGAATA CTTAGCAGAA GCCAGTGTTG TTTTCCCCAA GGTTTTTCCT
    GTTGTGCACA ATTTGTTGGA CTCTAAGATC AACACCTTGT TGTCATTGTG CCAAGATCCA
    AATTTGTTAA ATCCAATCCA TGGAATCGTG CAGAGTGTGG TATACCATGA AGAATCCCCA
    CCTCAGTACC AAACATCTTA TCTGCAAAGT TTTGGTTTTA ATGGCTTATG GCGGTTTGCA
    GGACCCTTTT CAAAGGAATC GACAAGGAGA ACGTTGAACT CTCCCCAACC ACTGGGCACT
    GTAACAGTGG ACGGACTCGC CATGGATCCG CAAGCCAAGT GCAGAAGCAA AGAAGCGCTG
    GCAGTTTCAA ACGTAATAGC ATTAAGAAGA TCGTGTGAAA CTTTCTTTCT TTCCTTTTTC
    AAACAGACTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38290
    Clone ID Related Accession (Same CDS sequence) XM_014116492.1
    Accession Version XM_014116492.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofibromin
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGTTGTTGC TTCCAATAAA AACAGGACAG CAGAACACGC ATACCAAAGT CAGTACTGAA 
    CACAACAAGG AATGTCTTAT TAATATTTCC AAATACAAGT TTTCTTTGGT TATCAGCGGC
    CTCACCACTA TCTTAAAGAA TGTTAACAAT ATGAGAATAT TTGGAGAAGC TGCTGAAAAA
    AATTTATATC TCTCTCAGTT GATTATATTG GATACACTGG AAAAATGTCT TGCTGGGCAA
    CCAAAGGACA CGATGAGATT AGATGAAACA ATGCTGGTCA AACAGTTGCT GCCGGAAATC
    TGCCATTTTC TTCATACCTG TCGTGAAGGA AACCAACATG CCGCTGAACT TCGGAATTCC
    GCCTCTGGGG TTTTATTTTC TCTCAGCTGC AACAACTTCA ATGCAGTGTT TAGTCGCATT
    TCTACCAGGT TACAGGAATT GACGGTTTGT TCAGAAGACA ATGTTGATGT TCATGATATA
    GAATTGTTAC AGTATATCAA TGTGGATTGT GCAAAATTAA AACGACTTCT AAAGGAAACT
    GCATTTAAAT TTAAAGCCCT AAAGAAGGTT GCACAGTTAG CAGTTATAAA TAGCCTGGAA
    AAAGCATTTT GGAACTGGGT AGAAAATTAT CCAGATGAAT TTACAAAGCT GTACCAGATT
    CCACAGACTG ATATGGCTGA GTGTGCGGAA AAACTGTTTG ACTTGGTGGA TGGTTTTGCT
    GAAAGCACCA AACGTAAAGC AGCAGTTTGG CCACTGCAAA TCATTCTCCT TATCTTGTGT
    CCAGAAATAA TTCAGGATAT ATCCAAGGAT GTGGTTGATG AAAACAACAT GAATAAGAAA
    TTGTTTCTGG ACAGTCTACG AAAAGCACTC GCCGGCCACG GAGGAAGCAG GCAGCTGACA
    GAAAGTGCTG CGATTGCCTG TGTCAAACTC TGTAAAGCAA GTACTTATAT CAATTGGGAA
    GATAACTCTG TCATTTTCCT ACTGGTTCAG TCCATGGTGG TTGATCTTAA GAACCTACTC
    TTTAATCCAA GTAAACCATT CTCAAGAGGC AGTCAGCCTG CAGATGTGGA TCTAATGATT
    GACTGCCTTG TTTCTTGCTT TCGAATAAGC CCTCACAATA ACCAACACTT TAAGATCTGC
    CTGGCTCAGA ATTCCCCTTC TACATTTCAC TATGTGCTGG TAAATTCACT CCATCGAATC
    ATCACCAATA GCACTTTCAA GCATGGACTT GGCACTGCTT CTGCATTGGA TTGGTGGCCT
    AAGATTGATG CTGTATATTG TCACTCAGTT GAGCTTCGAA ACATGTTTGG TGAAACACTT
    CATAAAGCAG TGCAGGGTTG TGGAGCACAC CCAGCAATAC GAATGGCACC GAGTCTTACA
    TTTAAAGAAA AAGTAACAAG CCTTAAATTT AAAGAAAAAC CTACAGACCT GGAGACAAGA
    AGCTATAAAT ATCTTCTCTT ATCCATGGTG AAACTAATTC ATGCAGATCC AAAGCTCTTG
    CTTTGTAATC CAAGAAAACA GGGACCTGAA ACCCAAGGCA GTACAGCGGA GTTAATTACA
    GGACTTGTCC AGCTGGTCCC TCAGTCACAT ATGCCAGAGA TTGCTCAGGA AGCAATGGAG
    GCTCTGCTGG TTCTTCATCA GTTAGACAGC ATTGATTTGT GGAATCCTGA TGCTCCTGTA
    GAAACATTTT GGGAGATTAG CTCTCAAATG CTTTTTTACA TCTGCAAGAA ATTAACTAGC
    CATCAGATGC TTAGTAGCAC AGAAATTCTC AAATGGTTGA GGGAGATTTT GATCTGCAGG
    AATAAATTTC TTCTTAAAAA TAAGCAGGCC GATAGAAGTT CCTGTCACTT TCTCTTCCTT
    TATGGGGTAG GATGTGATGT TCCTTCAAGT GGAACTACTA GTCAGATGTC CATGGATCAT
    GAAGAATTAC TACGTTCTTG TACTCCTGGA GCCTCTCTCC GGAAGGGAAA AGGAAACTCA
    TCCATGGATA GTGCAGCAGG GTGCAGCGGT ACTCCGCCAA TATGCCGACA AGCTCAGACT
    AAACTAGAAG TGGCCCTGTA CATGTTTCTG TGGAGCCCTG ACACTGAAGC TGTTCTGGTT
    GCCATGTCCT GTTTCCGTCA CCTTTGTGAG GAAGCAGATA TCCGGTGTGG GGTGGACGAA
    GTGTCGGTGC ACAACTTCTT GCCCAACTAT AACACATTTA TGGAATTTGC CTCTGTCAGC
    AATATGATGT CAACAGGAAG AGCAGCACTT CAGAAAAGAG TGATGGCACT ACTAAGGCGC
    ATTGAGCATC CCACTGCAGG AAATACCGAG GCTTGGGAAG ATACACATGC GAAGTGGGAA
    CAAGCTACAA AACTAATCCT CAACTACCCA AAAGCTAAAA TGGAAGATGG CCAGGCTGCT
    GAAAGCCTTC ACAAGACCAT TGTTAAGAGG CGAATGTCTC ATGTCAGTGG AGGAGGATCC
    ATAGATTTGT CTGACACGGA CTCCCTACAG GAATGGATCA ACATGACTGG CTTCCTCTGT
    GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA AGCAATTCTG GCTTGGCAAC CTATAGCCCA
    CCCATGGGCC CAGTCAGTGA ACGTAAGGGT TCCATGATTT CAGTGATGTC TTCAGAGGGA
    AATGCAGATA CACCTGTCAG CAAATTTATG GATCGGCTGT TATCCTTAAT GGTGTGTAAC
    CATGAGAAAG TGGGACTTCA AATACGGACC AATGTTAAGG ATCTGGTGGG TCTAGAATTG
    AGTCCTGCTC TTTATCCAAT GCTATTTAAC AAATTGAAGA ATACCATCAG CAAGTTTTTT
    GACTCCCAAG GACAGGTTTT ATTGACTGAC ACCAATACTC AGTTTGTGGA GCAAACCATA
    GCTATAATGA AGAACTTACT AGATAATCAT ACTGAAGGCA GCTCTGAACA CCTGGGGCAA
    GCTAGCATTG AAACAATGAT GTTAAATCTA GTCAGATATG TTCGTGTGCT TGGGAATATG
    GTCCATGCAA TTCAAATAAA AACGAAACTC TGTCAGTTAG TCGAGGTGAT GATGGCGAGG
    AGAGATGACC TCTCATTTTG TCAAGAGATG AAATTTAGGA ATAAGATGGT AGAATACCTG
    ACAGACTGGG TTATGGGAAC ATCCAACCAG GCAGCAGATG ATGATGTAAA ATGTCTTACA
    AGAGACTTGG ACCAGGCAAG CATGGAAGCA GTAGTTTCAC TCCTGGCTGG TCTTCCACTG
    CAGCCGGAGG AAGGAGATGG TGTGGAATTG ATGGAAGCCA AGTCACAGTT ATTTCTTAAG
    TATTTCACAT TGTTCATGAA CCTATTGAAT GACTGTAGTG AAGTTGAAGA TGAAAATGCA
    CAAACAGGTG GCAGGAAACG TGGCATGTCT CGGAGGCTGG CATCACTGAG GCACTGTACA
    GTCCTTGCAA TGTCAAACTT ACTCAATGCT AATGTGGACA GTGGCCTCAT GCATTCCATA
    GGTTTAGGTT ATCACAAAGA TCTTCAGACA AGAGCTACAT TTATGGAAGT TCTGACAAAA
    ATCCTCCAAC AAGGCACAGA ATTTGACACA CTTGCGGAAA CAGTGCTGGC CGACCGGTTT
    GAGAGGCTGG TGGAGTTGGT GACGATGATG GGAGACCAGG GAGAGCTCCC AATAGCCATG
    GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG TGGGATGAAC TAGCTCGAGT TTTGGTGACT
    CTGTTTGATT CTCGGCATTT ACTCTACCAA TTGCTCTGGA ACATGTTTTC TAAGGAAGTA
    GAATTGGCAG ACTCCATGCA GACTCTTTTC CGAGGAAACA GCTTAGCCAG TAAAATAATG
    ACCTTCTGTT TCAAGGTATA TGGTGCTACC TACCTACAAA AACTCTTGGA TCCGTTATTA
    CGAATTGTGA TCACATCCTC TGATTGGCAG CATGTTAGCT TTGAAGTGGA TCCTACCAGA
    TTAGAACAGT CAGAGAGCCT TGAGGAAAAC CAACGGAACC TTCTTCAGAT GACTGAAAAG
    TTCTTCCATG CCATCATCAG TTCCTCCTCA GAATTCCCCC CTCAGCTTCG AAGTGTGTGC
    CACTGTTTAT ACCAGGCAAC TTGCCACTCC CTACTGAATA AAGCTACAGT AAAAGAAAAA
    AAGGAAAACA AAAAATCAGT GGTTAGCCAG CGTTTCCCTC AGAACAGCAT CGGTGCAGTG
    GGAAGTGCCA TGTTCCTCAG ATTTATCAAT CCTGCCATTG TCTCACCGTA CGAAGCAGGG
    ATTTTAGATA AAAAGCCACC ACCTAGAATT GAAAGGGGCT TGAAGTTAAT GTCAAAGATA
    CTTCAGAGTA TTGCCAATCA TGTTCTTTTC ACAAAAGAAG AGCATATGCG GCCTTTTAAT
    GATTTTGTGA AGAGCAACTT TGATGCAGCA CGGAGGTTTT TCCTTGATAT AGCATCTGAT
    TGTCCTACAA GTGATACAGT AAATCATAGT CTTTCCTTCA TCAGTGATGG CAATGTGCTT
    GCTTTACATC GTCTACTCTG GAACAATCAA GAGAAAATTG GCCAGTATCT TTCCAGCAAC
    AGGGATCACA AAGCTGTTGG AAGACGACCT TTCGATAAGA TGGCAACACT TCTTGCATAC
    CTGGGTCCTC CAGAGCACAA ACCTGTGGCA GATACACACT GGTCCAGCCT TAACCTTACC
    AGTTCAAAGT TTGAGGAATT TATGACTAGG CATCAAGTAC ATGAAAAAGA AGAGTTCAAG
    GCTTTGAAAA CATTAAGTAT TTTCTACCAA GCAGGGACTT CCAAAGCTGG GAATCCTATA
    TTTTATTATG TTGCACGGAG GTTTAAAACT GGTCAAATCA ATGGCGATTT GCTGATATAC
    CATGTCTTGC TGACTTTAAA GCCATATTAT GCAAAGCCAT ATGAAATTGT AGTGGACCTT
    ACGCATACTG GGCCGAGCAA TCGCTTTAAA ACAGACTTTC TCTCCAAGTG GTTTGTTGTT
    TTTCCTGGCT TCGCTTATGA CAATGTCTCT GCAGTCTATA TCTATAACTG TAACTCCTGG
    GTCAGAGAGT ACACCAAGTA TCACGAGCGG CTGCTGACTG GCCTCAAAGG CAGCAAAAGG
    CTCATCTTCA TAGACTGTCC TGGGAAACTG GCTGAGCACA TAGAGCATGA ACAACAGAAA
    CTGCCTGCTG CCACACTGGC TTTAGAAGAG GACCTGAAGG TATTCCACAA TGCTCTCAAG
    CTAGCTCACA AAGACACCAA GGTTTCCATT AAAGTTGGTT CTACTGCTGT TCAAGTAACC
    TCAGCAGAGC GAACAAAAGT CCTGGGGCAA TCGGTCTTTC TAAATGATAT TTATTATGCT
    TCGGAAATTG AAGAGATCTG CCTGGTAGAT GAGAACCAGT TTACCTTAAC CATTGCAAAC
    CAGGGCACAC CACTCACCTT CATGCACCAG GAGTGTGAAG CCATTGTTCA GTCTATCATC
    CACATCCGGA CACGCTGGGA GCTGTCACAA CCTGACTCCA TCCCCCAACA CACCAAGATT
    CGGCCAAAAG ATGTCCCTGG GACGCTGCTC AATATTGCGT TACTTAATTT AGGCAGTTCA
    GACCCTAGTT TACGGTCAGC TGCCTATAAT CTTCTGTGTG CCTTAACTTG TACCTTCAAT
    TTAAAAATCG AGGGCCAATT ACTAGAGACA TCAGGTTTAT GCATCCCTGC CAACAACACC
    CTCTTTATTG TCTCTATTAG TAAGACACTG GCAGCCAATG AGCCACATCT GACCTTAGAA
    TTTTTGGAAG AGTGTATTTC TGGATTTAGC AAGTCTAGTA TTGAATTGAA ACACCTTTGT
    TTGGAATACA TGACTCCGTG GCTGTCAAAT CTAGTCCGTT TTTGTAAGCA CAATGATGAT
    GCCAAAAGAC AGAGAGTTAC TGCTATTCTT GATAAGCTGA TAACAATGAC CATAAATGAG
    AAACAGATGT ACCCATCTAT CCAGGCCAAA ATATGGGGGA GCCTTGGGCA GATTACAGAT
    CTCCTTGATG TTGTATTAGA CAGTTTCATC AAAACCAGTG CAACAGGTGG CTTGGGATCA
    ATAAAAGCTG AGGTGATGGC AGATACTGCT GTAGCTTTGG CTTCTGGAAA CGTGAAATTG
    GTTTCAAGCA AGGTTATTGG AAGGATGTGC AAAATAATTG ACAAGACATG TTTATCTCCA
    ACTCCTACCT TAGAGCAACA TCTTATGTGG GACGATATTG CTATTCTAGC ACGCTACATG
    CTGATGCTGT CCTTCAACAA TTCCCTTGAT GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT
    GTTGTTACTT TCTTAGTAGC TACAGGGCCA CTCTCCCTTA GAGCTTCCAC ACATGGATTG
    GTCATTAATA TCATTCACTC TCTGTGTACT TGTTCACAGC TTCATTTTAG TGAAGAGACA
    AAGCAAGTTT TGAGACTCAG TTTGACAGAG TTCTCATTAC CCAAGTTTTA CCTGCTGTTT
    GGCATTAGTA AAGTCAAGTC AGCTGCCGTC ATTGCCTTTC GCTCCAGTTA CCGGGACAGG
    TCATTCTCTC CTGGCTCCTA TGAAAGGGAG ACTTTTGCTT TGACATCCTT GGAAACAGTC
    ACAGAAGCTT TGTTGGAGAT TATGGAGGCA TGTATGAGAG ATATTCCAAC ATGCAAGTGG
    CTGGACCAGT GGACAGAACT AGCTCAAAGA TTTGCATTCC AATATAATCC ATCCCTGCAA
    CCAAGAGCTC TTGTTGTCTT TGGCTGTATT AGCAAACGAG TGTCTCATGG GCAGATAAAA
    CAGATTATCC GTATTCTTAG CAAGGCACTT GAGAGTTGCT TAAAAGGACC TGATACTTAC
    AACAGTCAAG TTCTGATAGA AGCTACAGTA ATAGCACTAA CCAAATTACA ACCACTTCTT
    AATAAGGACT CCCCTCTGCA CAAAGCCCTC TTTTGGGTGG CTGTGGCTGT GCTACAGCTC
    GATGAGGTCA ACCTGTATTC AGCAGGCAGT GCGCTTCTGG AACAAAACCT GCACACCTTA
    GACAGTCTGC GGGTATTCAA TGACAAGAGT CCAGAGGAAG TATTTATGGC AATCCGGAAT
    CCTCTGGAAT GGCACTGCAA ACAAATGGAT CATTTTGTTG GACTCAATTT CAACTCTAAC
    TTTAACTTCG CACTGGTTGG ACACCTCTTA AAAGGGTACA GGCATCCTTC ACCTGCCATT
    GTGGCAAGAA CAGTCAGAAT TTTGCATACA CTACTAACTC TGGTGAACAA ACATAGAAAC
    TGTGACAAAT TTGAGGTGAA CACACAGAGC GTGGCTTATT TAGCAGCCTT ACTCACAGTG
    TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA AAACATAGAA AATCTCTTCT TCTTACTGAT
    ATTTCAATGG AAAATGTTCC TATGGATACG TATCCCATCC ATCATGGCGA CCCTAGCTAT
    AGGACGCTAA AGGAGAACCA GCCGTGGTCA TCTCCCAAAG GTTCTGAAGG GTACCTTGCA
    GCCTCCTATC CAGCCGTGGG CCAGACCAGT CCCCGAGCCA GGAAATCCAT GAGTTTGGAC
    ATGGGGCAGC CTTCTCAGGC CAATACGAAG AAGTTGCTGG GAACAAGGAA AAGTTTTGAT
    CACTTGATAT CAGACACAAA GGCTCCTAAA AGGCAAGAAA TGGAATCAGG GATCACAACA
    CCCCCCAAAA TGAGGAGAGT AGCAGAAACT GATTATGAAA TGGAAACTCA GAGGATTTCC
    TCATCACAGC AACACCCACA TTTACGCAAA GTTTCAGTGT CTGAATCAAA TGTTCTCTTG
    GATGAAGAAG TACTTACTGA TCCGAAGATC CAAGCACTTC TTCTTACTGT TCTGGCTACA
    CTGGTAAAAT ACACCACAGA TGAGTTTGAT CAGCGAATTC TTTATGAATA CTTAGCAGAA
    GCCAGTGTTG TTTTCCCCAA GGTTTTTCCT GTTGTGCACA ATTTGTTGGA CTCTAAGATC
    AACACCTTGT TGTCATTGTG CCAAGATCCA AATTTGTTAA ATCCAATCCA TGGAATCGTG
    CAGAGTGTGG TATACCATGA AGAATCCCCA CCTCAGTACC AAACATCTTA TCTGCAAAGT
    TTTGGTTTTA ATGGCTTATG GCGGTTTGCA GGACCCTTTT CAAAGCAAAC ACAAATCCCA
    GACTATGCTG AGCTTATTGT TAAGTTTCTT GATGCCTTGA TTGACACGTA TTTGCCTGGA
    ATTGATGAAG AAACCAGTGA GGAGTCCCTC CTGACACCCA CTTCTCCTTA TCCTCCTGCA
    GTGCAGAGCC AGCTTAGTAT TACTGCTAAT CTTAACCTTT CTAATTCCAT GACCTCACTT
    GCAACTTCCC AGCATTCCCC AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCAACCACT
    GGGCACTGTA ACAGTGGACG GACTCGCCAT GGATCCGCAA GCCAAGTGCA GAAGCAAAGA
    AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013971967.1
    CDS1..8505
    Translation

    Target ORF information:

    RefSeq Version XM_014116492.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofibromin 1 (NF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014116492.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGTTGTTGC TTCCAATAAA AACAGGACAG CAGAACACGC ATACCAAAGT CAGTACTGAA 
    CACAACAAGG AATGTCTTAT TAATATTTCC AAATACAAGT TTTCTTTGGT TATCAGCGGC
    CTCACCACTA TCTTAAAGAA TGTTAACAAT ATGAGAATAT TTGGAGAAGC TGCTGAAAAA
    AATTTATATC TCTCTCAGTT GATTATATTG GATACACTGG AAAAATGTCT TGCTGGGCAA
    CCAAAGGACA CGATGAGATT AGATGAAACA ATGCTGGTCA AACAGTTGCT GCCGGAAATC
    TGCCATTTTC TTCATACCTG TCGTGAAGGA AACCAACATG CCGCTGAACT TCGGAATTCC
    GCCTCTGGGG TTTTATTTTC TCTCAGCTGC AACAACTTCA ATGCAGTGTT TAGTCGCATT
    TCTACCAGGT TACAGGAATT GACGGTTTGT TCAGAAGACA ATGTTGATGT TCATGATATA
    GAATTGTTAC AGTATATCAA TGTGGATTGT GCAAAATTAA AACGACTTCT AAAGGAAACT
    GCATTTAAAT TTAAAGCCCT AAAGAAGGTT GCACAGTTAG CAGTTATAAA TAGCCTGGAA
    AAAGCATTTT GGAACTGGGT AGAAAATTAT CCAGATGAAT TTACAAAGCT GTACCAGATT
    CCACAGACTG ATATGGCTGA GTGTGCGGAA AAACTGTTTG ACTTGGTGGA TGGTTTTGCT
    GAAAGCACCA AACGTAAAGC AGCAGTTTGG CCACTGCAAA TCATTCTCCT TATCTTGTGT
    CCAGAAATAA TTCAGGATAT ATCCAAGGAT GTGGTTGATG AAAACAACAT GAATAAGAAA
    TTGTTTCTGG ACAGTCTACG AAAAGCACTC GCCGGCCACG GAGGAAGCAG GCAGCTGACA
    GAAAGTGCTG CGATTGCCTG TGTCAAACTC TGTAAAGCAA GTACTTATAT CAATTGGGAA
    GATAACTCTG TCATTTTCCT ACTGGTTCAG TCCATGGTGG TTGATCTTAA GAACCTACTC
    TTTAATCCAA GTAAACCATT CTCAAGAGGC AGTCAGCCTG CAGATGTGGA TCTAATGATT
    GACTGCCTTG TTTCTTGCTT TCGAATAAGC CCTCACAATA ACCAACACTT TAAGATCTGC
    CTGGCTCAGA ATTCCCCTTC TACATTTCAC TATGTGCTGG TAAATTCACT CCATCGAATC
    ATCACCAATA GCACTTTCAA GCATGGACTT GGCACTGCTT CTGCATTGGA TTGGTGGCCT
    AAGATTGATG CTGTATATTG TCACTCAGTT GAGCTTCGAA ACATGTTTGG TGAAACACTT
    CATAAAGCAG TGCAGGGTTG TGGAGCACAC CCAGCAATAC GAATGGCACC GAGTCTTACA
    TTTAAAGAAA AAGTAACAAG CCTTAAATTT AAAGAAAAAC CTACAGACCT GGAGACAAGA
    AGCTATAAAT ATCTTCTCTT ATCCATGGTG AAACTAATTC ATGCAGATCC AAAGCTCTTG
    CTTTGTAATC CAAGAAAACA GGGACCTGAA ACCCAAGGCA GTACAGCGGA GTTAATTACA
    GGACTTGTCC AGCTGGTCCC TCAGTCACAT ATGCCAGAGA TTGCTCAGGA AGCAATGGAG
    GCTCTGCTGG TTCTTCATCA GTTAGACAGC ATTGATTTGT GGAATCCTGA TGCTCCTGTA
    GAAACATTTT GGGAGATTAG CTCTCAAATG CTTTTTTACA TCTGCAAGAA ATTAACTAGC
    CATCAGATGC TTAGTAGCAC AGAAATTCTC AAATGGTTGA GGGAGATTTT GATCTGCAGG
    AATAAATTTC TTCTTAAAAA TAAGCAGGCC GATAGAAGTT CCTGTCACTT TCTCTTCCTT
    TATGGGGTAG GATGTGATGT TCCTTCAAGT GGAACTACTA GTCAGATGTC CATGGATCAT
    GAAGAATTAC TACGTTCTTG TACTCCTGGA GCCTCTCTCC GGAAGGGAAA AGGAAACTCA
    TCCATGGATA GTGCAGCAGG GTGCAGCGGT ACTCCGCCAA TATGCCGACA AGCTCAGACT
    AAACTAGAAG TGGCCCTGTA CATGTTTCTG TGGAGCCCTG ACACTGAAGC TGTTCTGGTT
    GCCATGTCCT GTTTCCGTCA CCTTTGTGAG GAAGCAGATA TCCGGTGTGG GGTGGACGAA
    GTGTCGGTGC ACAACTTCTT GCCCAACTAT AACACATTTA TGGAATTTGC CTCTGTCAGC
    AATATGATGT CAACAGGAAG AGCAGCACTT CAGAAAAGAG TGATGGCACT ACTAAGGCGC
    ATTGAGCATC CCACTGCAGG AAATACCGAG GCTTGGGAAG ATACACATGC GAAGTGGGAA
    CAAGCTACAA AACTAATCCT CAACTACCCA AAAGCTAAAA TGGAAGATGG CCAGGCTGCT
    GAAAGCCTTC ACAAGACCAT TGTTAAGAGG CGAATGTCTC ATGTCAGTGG AGGAGGATCC
    ATAGATTTGT CTGACACGGA CTCCCTACAG GAATGGATCA ACATGACTGG CTTCCTCTGT
    GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA AGCAATTCTG GCTTGGCAAC CTATAGCCCA
    CCCATGGGCC CAGTCAGTGA ACGTAAGGGT TCCATGATTT CAGTGATGTC TTCAGAGGGA
    AATGCAGATA CACCTGTCAG CAAATTTATG GATCGGCTGT TATCCTTAAT GGTGTGTAAC
    CATGAGAAAG TGGGACTTCA AATACGGACC AATGTTAAGG ATCTGGTGGG TCTAGAATTG
    AGTCCTGCTC TTTATCCAAT GCTATTTAAC AAATTGAAGA ATACCATCAG CAAGTTTTTT
    GACTCCCAAG GACAGGTTTT ATTGACTGAC ACCAATACTC AGTTTGTGGA GCAAACCATA
    GCTATAATGA AGAACTTACT AGATAATCAT ACTGAAGGCA GCTCTGAACA CCTGGGGCAA
    GCTAGCATTG AAACAATGAT GTTAAATCTA GTCAGATATG TTCGTGTGCT TGGGAATATG
    GTCCATGCAA TTCAAATAAA AACGAAACTC TGTCAGTTAG TCGAGGTGAT GATGGCGAGG
    AGAGATGACC TCTCATTTTG TCAAGAGATG AAATTTAGGA ATAAGATGGT AGAATACCTG
    ACAGACTGGG TTATGGGAAC ATCCAACCAG GCAGCAGATG ATGATGTAAA ATGTCTTACA
    AGAGACTTGG ACCAGGCAAG CATGGAAGCA GTAGTTTCAC TCCTGGCTGG TCTTCCACTG
    CAGCCGGAGG AAGGAGATGG TGTGGAATTG ATGGAAGCCA AGTCACAGTT ATTTCTTAAG
    TATTTCACAT TGTTCATGAA CCTATTGAAT GACTGTAGTG AAGTTGAAGA TGAAAATGCA
    CAAACAGGTG GCAGGAAACG TGGCATGTCT CGGAGGCTGG CATCACTGAG GCACTGTACA
    GTCCTTGCAA TGTCAAACTT ACTCAATGCT AATGTGGACA GTGGCCTCAT GCATTCCATA
    GGTTTAGGTT ATCACAAAGA TCTTCAGACA AGAGCTACAT TTATGGAAGT TCTGACAAAA
    ATCCTCCAAC AAGGCACAGA ATTTGACACA CTTGCGGAAA CAGTGCTGGC CGACCGGTTT
    GAGAGGCTGG TGGAGTTGGT GACGATGATG GGAGACCAGG GAGAGCTCCC AATAGCCATG
    GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG TGGGATGAAC TAGCTCGAGT TTTGGTGACT
    CTGTTTGATT CTCGGCATTT ACTCTACCAA TTGCTCTGGA ACATGTTTTC TAAGGAAGTA
    GAATTGGCAG ACTCCATGCA GACTCTTTTC CGAGGAAACA GCTTAGCCAG TAAAATAATG
    ACCTTCTGTT TCAAGGTATA TGGTGCTACC TACCTACAAA AACTCTTGGA TCCGTTATTA
    CGAATTGTGA TCACATCCTC TGATTGGCAG CATGTTAGCT TTGAAGTGGA TCCTACCAGA
    TTAGAACAGT CAGAGAGCCT TGAGGAAAAC CAACGGAACC TTCTTCAGAT GACTGAAAAG
    TTCTTCCATG CCATCATCAG TTCCTCCTCA GAATTCCCCC CTCAGCTTCG AAGTGTGTGC
    CACTGTTTAT ACCAGGCAAC TTGCCACTCC CTACTGAATA AAGCTACAGT AAAAGAAAAA
    AAGGAAAACA AAAAATCAGT GGTTAGCCAG CGTTTCCCTC AGAACAGCAT CGGTGCAGTG
    GGAAGTGCCA TGTTCCTCAG ATTTATCAAT CCTGCCATTG TCTCACCGTA CGAAGCAGGG
    ATTTTAGATA AAAAGCCACC ACCTAGAATT GAAAGGGGCT TGAAGTTAAT GTCAAAGATA
    CTTCAGAGTA TTGCCAATCA TGTTCTTTTC ACAAAAGAAG AGCATATGCG GCCTTTTAAT
    GATTTTGTGA AGAGCAACTT TGATGCAGCA CGGAGGTTTT TCCTTGATAT AGCATCTGAT
    TGTCCTACAA GTGATACAGT AAATCATAGT CTTTCCTTCA TCAGTGATGG CAATGTGCTT
    GCTTTACATC GTCTACTCTG GAACAATCAA GAGAAAATTG GCCAGTATCT TTCCAGCAAC
    AGGGATCACA AAGCTGTTGG AAGACGACCT TTCGATAAGA TGGCAACACT TCTTGCATAC
    CTGGGTCCTC CAGAGCACAA ACCTGTGGCA GATACACACT GGTCCAGCCT TAACCTTACC
    AGTTCAAAGT TTGAGGAATT TATGACTAGG CATCAAGTAC ATGAAAAAGA AGAGTTCAAG
    GCTTTGAAAA CATTAAGTAT TTTCTACCAA GCAGGGACTT CCAAAGCTGG GAATCCTATA
    TTTTATTATG TTGCACGGAG GTTTAAAACT GGTCAAATCA ATGGCGATTT GCTGATATAC
    CATGTCTTGC TGACTTTAAA GCCATATTAT GCAAAGCCAT ATGAAATTGT AGTGGACCTT
    ACGCATACTG GGCCGAGCAA TCGCTTTAAA ACAGACTTTC TCTCCAAGTG GTTTGTTGTT
    TTTCCTGGCT TCGCTTATGA CAATGTCTCT GCAGTCTATA TCTATAACTG TAACTCCTGG
    GTCAGAGAGT ACACCAAGTA TCACGAGCGG CTGCTGACTG GCCTCAAAGG CAGCAAAAGG
    CTCATCTTCA TAGACTGTCC TGGGAAACTG GCTGAGCACA TAGAGCATGA ACAACAGAAA
    CTGCCTGCTG CCACACTGGC TTTAGAAGAG GACCTGAAGG TATTCCACAA TGCTCTCAAG
    CTAGCTCACA AAGACACCAA GGTTTCCATT AAAGTTGGTT CTACTGCTGT TCAAGTAACC
    TCAGCAGAGC GAACAAAAGT CCTGGGGCAA TCGGTCTTTC TAAATGATAT TTATTATGCT
    TCGGAAATTG AAGAGATCTG CCTGGTAGAT GAGAACCAGT TTACCTTAAC CATTGCAAAC
    CAGGGCACAC CACTCACCTT CATGCACCAG GAGTGTGAAG CCATTGTTCA GTCTATCATC
    CACATCCGGA CACGCTGGGA GCTGTCACAA CCTGACTCCA TCCCCCAACA CACCAAGATT
    CGGCCAAAAG ATGTCCCTGG GACGCTGCTC AATATTGCGT TACTTAATTT AGGCAGTTCA
    GACCCTAGTT TACGGTCAGC TGCCTATAAT CTTCTGTGTG CCTTAACTTG TACCTTCAAT
    TTAAAAATCG AGGGCCAATT ACTAGAGACA TCAGGTTTAT GCATCCCTGC CAACAACACC
    CTCTTTATTG TCTCTATTAG TAAGACACTG GCAGCCAATG AGCCACATCT GACCTTAGAA
    TTTTTGGAAG AGTGTATTTC TGGATTTAGC AAGTCTAGTA TTGAATTGAA ACACCTTTGT
    TTGGAATACA TGACTCCGTG GCTGTCAAAT CTAGTCCGTT TTTGTAAGCA CAATGATGAT
    GCCAAAAGAC AGAGAGTTAC TGCTATTCTT GATAAGCTGA TAACAATGAC CATAAATGAG
    AAACAGATGT ACCCATCTAT CCAGGCCAAA ATATGGGGGA GCCTTGGGCA GATTACAGAT
    CTCCTTGATG TTGTATTAGA CAGTTTCATC AAAACCAGTG CAACAGGTGG CTTGGGATCA
    ATAAAAGCTG AGGTGATGGC AGATACTGCT GTAGCTTTGG CTTCTGGAAA CGTGAAATTG
    GTTTCAAGCA AGGTTATTGG AAGGATGTGC AAAATAATTG ACAAGACATG TTTATCTCCA
    ACTCCTACCT TAGAGCAACA TCTTATGTGG GACGATATTG CTATTCTAGC ACGCTACATG
    CTGATGCTGT CCTTCAACAA TTCCCTTGAT GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT
    GTTGTTACTT TCTTAGTAGC TACAGGGCCA CTCTCCCTTA GAGCTTCCAC ACATGGATTG
    GTCATTAATA TCATTCACTC TCTGTGTACT TGTTCACAGC TTCATTTTAG TGAAGAGACA
    AAGCAAGTTT TGAGACTCAG TTTGACAGAG TTCTCATTAC CCAAGTTTTA CCTGCTGTTT
    GGCATTAGTA AAGTCAAGTC AGCTGCCGTC ATTGCCTTTC GCTCCAGTTA CCGGGACAGG
    TCATTCTCTC CTGGCTCCTA TGAAAGGGAG ACTTTTGCTT TGACATCCTT GGAAACAGTC
    ACAGAAGCTT TGTTGGAGAT TATGGAGGCA TGTATGAGAG ATATTCCAAC ATGCAAGTGG
    CTGGACCAGT GGACAGAACT AGCTCAAAGA TTTGCATTCC AATATAATCC ATCCCTGCAA
    CCAAGAGCTC TTGTTGTCTT TGGCTGTATT AGCAAACGAG TGTCTCATGG GCAGATAAAA
    CAGATTATCC GTATTCTTAG CAAGGCACTT GAGAGTTGCT TAAAAGGACC TGATACTTAC
    AACAGTCAAG TTCTGATAGA AGCTACAGTA ATAGCACTAA CCAAATTACA ACCACTTCTT
    AATAAGGACT CCCCTCTGCA CAAAGCCCTC TTTTGGGTGG CTGTGGCTGT GCTACAGCTC
    GATGAGGTCA ACCTGTATTC AGCAGGCAGT GCGCTTCTGG AACAAAACCT GCACACCTTA
    GACAGTCTGC GGGTATTCAA TGACAAGAGT CCAGAGGAAG TATTTATGGC AATCCGGAAT
    CCTCTGGAAT GGCACTGCAA ACAAATGGAT CATTTTGTTG GACTCAATTT CAACTCTAAC
    TTTAACTTCG CACTGGTTGG ACACCTCTTA AAAGGGTACA GGCATCCTTC ACCTGCCATT
    GTGGCAAGAA CAGTCAGAAT TTTGCATACA CTACTAACTC TGGTGAACAA ACATAGAAAC
    TGTGACAAAT TTGAGGTGAA CACACAGAGC GTGGCTTATT TAGCAGCCTT ACTCACAGTG
    TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA AAACATAGAA AATCTCTTCT TCTTACTGAT
    ATTTCAATGG AAAATGTTCC TATGGATACG TATCCCATCC ATCATGGCGA CCCTAGCTAT
    AGGACGCTAA AGGAGAACCA GCCGTGGTCA TCTCCCAAAG GTTCTGAAGG GTACCTTGCA
    GCCTCCTATC CAGCCGTGGG CCAGACCAGT CCCCGAGCCA GGAAATCCAT GAGTTTGGAC
    ATGGGGCAGC CTTCTCAGGC CAATACGAAG AAGTTGCTGG GAACAAGGAA AAGTTTTGAT
    CACTTGATAT CAGACACAAA GGCTCCTAAA AGGCAAGAAA TGGAATCAGG GATCACAACA
    CCCCCCAAAA TGAGGAGAGT AGCAGAAACT GATTATGAAA TGGAAACTCA GAGGATTTCC
    TCATCACAGC AACACCCACA TTTACGCAAA GTTTCAGTGT CTGAATCAAA TGTTCTCTTG
    GATGAAGAAG TACTTACTGA TCCGAAGATC CAAGCACTTC TTCTTACTGT TCTGGCTACA
    CTGGTAAAAT ACACCACAGA TGAGTTTGAT CAGCGAATTC TTTATGAATA CTTAGCAGAA
    GCCAGTGTTG TTTTCCCCAA GGTTTTTCCT GTTGTGCACA ATTTGTTGGA CTCTAAGATC
    AACACCTTGT TGTCATTGTG CCAAGATCCA AATTTGTTAA ATCCAATCCA TGGAATCGTG
    CAGAGTGTGG TATACCATGA AGAATCCCCA CCTCAGTACC AAACATCTTA TCTGCAAAGT
    TTTGGTTTTA ATGGCTTATG GCGGTTTGCA GGACCCTTTT CAAAGCAAAC ACAAATCCCA
    GACTATGCTG AGCTTATTGT TAAGTTTCTT GATGCCTTGA TTGACACGTA TTTGCCTGGA
    ATTGATGAAG AAACCAGTGA GGAGTCCCTC CTGACACCCA CTTCTCCTTA TCCTCCTGCA
    GTGCAGAGCC AGCTTAGTAT TACTGCTAAT CTTAACCTTT CTAATTCCAT GACCTCACTT
    GCAACTTCCC AGCATTCCCC AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCAACCACT
    GGGCACTGTA ACAGTGGACG GACTCGCCAT GGATCCGCAA GCCAAGTGCA GAAGCAAAGA
    AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.