CDKN2B cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	CDKN2B
Entrez Gene ID	395076
Full Name	cyclin dependent kinase inhibitor 2B
Synonyms	CDKN2A
General protein information	Preferred Names cyclin dependent kinase inhibitor 2B Names cyclin-dependent kinase 4 inhibitor B cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) cyclin-dependent kinase inhibitor 2B (melanoma, p16, inhibits CDK4) cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) p15INK4b tumor suppressor
Gene Type	protein-coding
Organism	Gallus gallus(chicken)
Summary	This gene encodes a protein similar to one of two mammalian cyclin-dependent kinase inhibitor proteins which regulate the activity of cyclin-dependent protein kinases CDK4 and CDK6 which further cell cycle progression through G1. Expression of this protein in human keratinocytes is induced by TGF-beta, a cytokine that inhibits cell growth through arrest in the G1 phase of the cell cycle. There is evidence that the encoded protein is functionally equivalent to mammalian p16INK4a, a protein from a similar but distinct mammalian gene. [provided by RefSeq, Dec 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_204433.1	NP_989764.1	cyclin-dependent kinase 4 inhibitor B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CDKN2B	NP_001139741.1
Mus musculus (house mouse)	Cdkn2b	NP_031696.1
Rattus norvegicus (Norway rat)	Cdkn2b	NP_570825.1
Bos taurus (cattle)	CDKN2B	NP_001069362.1
Homo sapiens (human)	CDKN2B	NP_004927.2
Pan troglodytes (chimpanzee)	CDKN2B	XP_003312069.1
	CDKN2B	XP_001107263.1
Gallus gallus (chicken)	CDKN2B	NP_989764.1

Gene Ontology
Bibliography

Related articles in PubMed

DNA methylation analysis of the gene CDKN2B in Gallus gallus (chicken).
Gryzi?ska M, Andraszek K, Jocek G
Folia biologica61(3-4)165-71(2013)

Gene pathways and cell cycle-related genes in cultured avian primordial germ cells.
Rengaraj D, Lee BR, Choi JW, Lee SI, Seo HW, Kim TH, Choi HJ, Song G, Han JY
Poultry science91(12)3167-77(2012 Dec)

Sex-linked barring in chickens is controlled by the CDKN2A /B tumour suppressor locus.
Hellstr?m AR, Sundstr?m E, Gunnarsson U, Bed'Hom B, Tixier-Boichard M, Honaker CF, Sahlqvist AS, Jensen P, K?mpe O, Siegel PB, Kerje S, Andersson L
Pigment cell & melanoma research23(4)521-30(2010 Aug)

Upregulation of chicken p15INK4b at senescence and in the developing brain.
Kim SH, Rowe J, Fujii H, Jones R, Schmierer B, Kong BW, Kuchler K, Foster D, Ish-Horowicz D, Peters G
Journal of cell science119(Pt 12)2435-43(2006 Jun)

Absence of p16INK4a and truncation of ARF tumor suppressors in chickens.
Kim SH, Mitchell M, Fujii H, Llanos S, Peters G
Proceedings of the National Academy of Sciences of the United States of America100(1)211-6(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The study was aimed at identifying the methylation of the CDKN2B gene situated at locus bar in Polbar chickens on the 6th and 18th day of embryonic development using the methylation-specific PCR method.
Title: DNA methylation analysis of the gene CDKN2B in Gallus gallus (chicken).

This novel finding provides the first evidence that the tumour suppressor locus CDKN2A can affect pigmentation phenotypes and sheds new light on the functional significance of this gene.
Title: Sex-linked barring in chickens is controlled by the CDKN2A /B tumour suppressor locus.

The INK4b transcript is expressed in the roof-plate of the developing hind-brain, consistent with a role in limiting cell proliferation.
Title: Upregulation of chicken p15INK4b at senescence and in the developing brain.

The following CDKN2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDKN2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa00551	NM_204433.1 Latest version!	Gallus gallus cyclin dependent kinase inhibitor 2B (CDKN2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDKN2B ( NM_204433.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989764.1 Gallus gallus cyclin dependent kinase inhibitor 2B (CDKN2B), mRNA.

Price & Availability↑

CloneID	OGa00551
Clone ID Related Accession (Same CDS sequence)	NM_204433.1
Accession Version	NM_204433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-08
Organism	Gallus gallus(chicken)
Product	cyclin-dependent kinase 4 inhibitor B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY138247.1. ##Evidence-Data-START## Transcript exon combination :: AY138247.1, AM065603.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCAGA GAGCGGCCAG CACGGCTGCG GATGAACTAG CCAACGCCGC CGCCCGCGGC 
GATCTGCTGC GGGTGAAGGA GCTACTGGAC GGCGCGGCCG ACCCCAACGC TGTCAACTCC 
TTCGGTCGGA CCCCCATCCA GGTGATGATG CTGGGCAGCC CTCGCGTGGC CGAGCTGCTG 
CTGCAGCGCG GCGCCGACCC CAACCGCCCC GACCCGCGCA CCGGCTGCCG CCCCGCACAC 
GACGCGGCCC GCGCCGGCTT CCTGGACACG CTGGCGGCGC TGCACCGCGC CGGGGCGCGC 
CTCGACCTGC CCGACGGCCG CGGCCGCCTC CCCATCGACG TGGCGGCCGG GGGCCCGCAC 
GGCCCGGTCG GCTGCTACCT GCGACGGCTG CCAGCGCTAC CGCGAGCACC CCTCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989764.1
CDS	191..610
Translation	MAQRAASTAADELANAAARGDLLRVKELLDGAADPNAVNSFGRTPIQVMMLGSPRVAELLLQRGADPNRPDPRTGCRPAHDAARAGFLDTLAALHRAGARLDLPDGRGRLPIDVAAGGPHGPVGCYLRRLPALPRAPLP

Target ORF information:

RefSeq Version	NM_204433.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus cyclin dependent kinase inhibitor 2B (CDKN2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204433.1

ORF Insert Sequence:

ATGGCGCAGA GAGCGGCCAG CACGGCTGCG GATGAACTAG CCAACGCCGC CGCCCGCGGC 
GATCTGCTGC GGGTGAAGGA GCTACTGGAC GGCGCGGCCG ACCCCAACGC TGTCAACTCC 
TTCGGTCGGA CCCCCATCCA GGTGATGATG CTGGGCAGCC CTCGCGTGGC CGAGCTGCTG 
CTGCAGCGCG GCGCCGACCC CAACCGCCCC GACCCGCGCA CCGGCTGCCG CCCCGCACAC 
GACGCGGCCC GCGCCGGCTT CCTGGACACG CTGGCGGCGC TGCACCGCGC CGGGGCGCGC 
CTCGACCTGC CCGACGGCCG CGGCCGCCTC CCCATCGACG TGGCGGCCGG GGGCCCGCAC 
GGCCCGGTCG GCTGCTACCT GCGACGGCTG CCAGCGCTAC CGCGAGCACC CCTCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DNA methylation analysis of the gene CDKN2B in Gallus gallus (chicken).
Folia biologica61(3-4)165-71(2013)
Gryzi?ska M,Andraszek K,Jocek G

Gene pathways and cell cycle-related genes in cultured avian primordial germ cells.
Poultry science91(12)3167-77(2012 Dec)
Rengaraj D,Lee BR,Choi JW,Lee SI,Seo HW,Kim TH,Choi HJ,Song G,Han JY

Sex-linked barring in chickens is controlled by the CDKN2A /B tumour suppressor locus.
Pigment cell & melanoma research23(4)521-30(2010 Aug)
Hellstr?m AR,Sundstr?m E,Gunnarsson U,Bed'Hom B,Tixier-Boichard M,Honaker CF,Sahlqvist AS,Jensen P,K?mpe O,Siegel PB,Kerje S,Andersson L

Upregulation of chicken p15INK4b at senescence and in the developing brain.
Journal of cell science119(Pt 12)2435-43(2006 Jun)
Kim SH,Rowe J,Fujii H,Jones R,Schmierer B,Kong BW,Kuchler K,Foster D,Ish-Horowicz D,Peters G

Absence of p16INK4a and truncation of ARF tumor suppressors in chickens.
Proceedings of the National Academy of Sciences of the United States of America100(1)211-6(2003 Jan)
Kim SH,Mitchell M,Fujii H,Llanos S,Peters G

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CDKN2B cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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