CDKN2B cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	CDKN2B
Entrez Gene ID	481563
Full Name	cyclin dependent kinase inhibitor 2B
Synonyms	p15INK4B
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005626410.2	XP_005626467.1	cyclin-dependent kinase 4 inhibitor B isoform X1
NM_001146269.1	NP_001139741.1	cyclin-dependent kinase 4 inhibitor B
XM_005626410.3	XP_005626467.1	cyclin-dependent kinase 4 inhibitor B isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CDKN2B	NP_001139741.1
Mus musculus (house mouse)	Cdkn2b	NP_031696.1
Rattus norvegicus (Norway rat)	Cdkn2b	NP_570825.1
Bos taurus (cattle)	CDKN2B	NP_001069362.1
Homo sapiens (human)	CDKN2B	NP_004927.2
Pan troglodytes (chimpanzee)	CDKN2B	XP_003312069.1
	CDKN2B	XP_001107263.1
Gallus gallus (chicken)	CDKN2B	NP_989764.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide analyses implicate 33 loci in heritable dog osteosarcoma, including regulatory variants near CDKN2A/B.
Karlsson EK, Sigurdsson S, Ivansson E, Thomas R, Elvers I, Wright J, Howald C, Tonomura N, Perloski M, Swofford R, Biagi T, Fryc S, Anderson N, Courtay-Cahen C, Youell L, Ricketts SL, Mandlebaum S, Rivera P, von Euler H, Kisseberth WC, London CA, Lander ES, Couto G, Comstock K, Starkey MP, Modiano JF, Breen M, Lindblad-Toh K
Genome biology14(12)R132(2013 Dec)

Simultaneous inactivation of the p16, p15 and p14 genes encoding cyclin-dependent kinase inhibitors in canine T-lymphoid tumor cells.
Fujiwara-Igarashi A, Goto-Koshino Y, Mochizuki H, Maeda S, Fujino Y, Ohno K, Tsujimoto H
The Journal of veterinary medical science75(6)733-42(2013)

Disruption of chromosome 11 in canine fibrosarcomas highlights an unusual variability of CDKN2B in dogs.
Aguirre-Hern?ndez J, Milne BS, Queen C, O'Brien PC, Hoather T, Haugland S, Ferguson-Smith MA, Dobson JM, Sargan DR
BMC veterinary research527(2009 Jul)

Inactivation of the p16 cyclin-dependent kinase inhibitor in high-grade canine non-Hodgkin's T-cell lymphoma.
Fosmire SP, Thomas R, Jubala CM, Wojcieszyn JW, Valli VE, Getzy DM, Smith TL, Gardner LA, Ritt MG, Bell JS, Freeman KP, Greenfield BE, Lana SE, Kisseberth WC, Helfand SC, Cutter GR, Breen M, Modiano JF
Veterinary pathology44(4)467-78(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The greyhound locus exhibiting the strongest association, located 150 kilobases upstream of the genes CDKN2A/B, is also the most rearranged locus in canine osteosarcoma tumors.
Title: Genome-wide analyses implicate 33 loci in heritable dog osteosarcoma, including regulatory variants near CDKN2A/B.

In this study, the full-length complementary DNA (cDNA) of the canine p16 gene was cloned using the 5' and 3' rapid amplification of cDNA ends methods.
Title: Simultaneous inactivation of the p16, p15 and p14 genes encoding cyclin-dependent kinase inhibitors in canine T-lymphoid tumor cells.

BAC hybridisations on metaphases of two fibrosarcomas showed loss of heterozygosity on chromosome 11 in the CDKN2B-CDKN2A tumour suppressor gene cluster region
Title: Disruption of chromosome 11 in canine fibrosarcomas highlights an unusual variability of CDKN2B in dogs.

The data show that p16 deletion or inactivation occurs almost exclusively in high-grade T-cell non-Hodgkin's lymphoma(NHL); alternative pathways can generate functional phenotypes of Rb deficiency in low-grade T-cell NHL and in high-grade B-cell NHL.
Title: Inactivation of the p16 cyclin-dependent kinase inhibitor in high-grade canine non-Hodgkin's T-cell lymphoma.

The following CDKN2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDKN2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe34321	NM_001146269.1 Latest version!	Canis lupus familiaris cyclin dependent kinase inhibitor 2B (CDKN2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$104.30 $149.00
OCe14144	XM_005626410.3 Latest version!	Canis lupus familiaris cyclin dependent kinase inhibitor 2B (CDKN2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$104.30 $149.00
View Old Accession Versions >>
OCe14144	XM_005626410.2	Canis lupus familiaris cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDKN2B ( NM_001146269.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001139741.1 Canis lupus familiaris cyclin dependent kinase inhibitor 2B (CDKN2B), mRNA.

Price & Availability↑

CloneID	OCe34321
Clone ID Related Accession (Same CDS sequence)	NM_001146269.1
Accession Version	NM_001146269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Canis lupus familiaris(dog)
Product	cyclin-dependent kinase 4 inhibitor B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAEX03007905.1. On Apr 4, 2009 this sequence version replaced XM_538685.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGCGCGAGG AGGCCACGGG CTTGCTCGGC GGGGACGGCG GCGACGCGGG CCTGGCCAGC 
GCCTCGGCGC AGGGGCAGGC GGAGACCGTG CGGCAGCTCC TGGAAGCCGG CGCGGATCCC 
AACGGAGTCA ACAGCTTCGG CAGGCGCCCG ATCCAGGTCA TGATGATGGG CAGCACCCGC 
GTGGCCCAGC TGCTGCTGCT CCACGGCGCC GACCCCAACT GTGCCGACCC CGCCACCCTC 
ACCCGCCCTG TGCACGACGC GGCCCGGGAG GGCTTCCTGG ACACGCTGGT GGTGCTGCAC 
CGAGCCGGGG CGCGGCTGGA CGTGCGCGAT GCCTGGGGCC GCCTGCCCGT GGACCTGGCT 
GAGGAGCGGG GCCACGGCGC TGTCGCCCGG TACCTGCGCG CAGCCGCGGG GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139741.1
CDS	1..417
Translation	MREEATGLLGGDGGDAGLASASAQGQAETVRQLLEAGADPNGVNSFGRRPIQVMMMGSTRVAQLLLLHGADPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEERGHGAVARYLRAAAGD

Target ORF information:

RefSeq Version	NM_001146269.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin dependent kinase inhibitor 2B (CDKN2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146269.1

ORF Insert Sequence:

ATGCGCGAGG AGGCCACGGG CTTGCTCGGC GGGGACGGCG GCGACGCGGG CCTGGCCAGC 
GCCTCGGCGC AGGGGCAGGC GGAGACCGTG CGGCAGCTCC TGGAAGCCGG CGCGGATCCC 
AACGGAGTCA ACAGCTTCGG CAGGCGCCCG ATCCAGGTCA TGATGATGGG CAGCACCCGC 
GTGGCCCAGC TGCTGCTGCT CCACGGCGCC GACCCCAACT GTGCCGACCC CGCCACCCTC 
ACCCGCCCTG TGCACGACGC GGCCCGGGAG GGCTTCCTGG ACACGCTGGT GGTGCTGCAC 
CGAGCCGGGG CGCGGCTGGA CGTGCGCGAT GCCTGGGGCC GCCTGCCCGT GGACCTGGCT 
GAGGAGCGGG GCCACGGCGC TGTCGCCCGG TACCTGCGCG CAGCCGCGGG GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDKN2B ( XM_005626410.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626467.1 Canis lupus familiaris cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe14144
Clone ID Related Accession (Same CDS sequence)	XM_005626410.2 , XM_005626410.3
Accession Version	XM_005626410.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cyclin-dependent kinase 4 inhibitor B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626410.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGAGG AGGCCACGGG CTTGCTCGGC GGCGGCGGCG GCGGGGACGG CGGCGGGGAC 
GGCGGCGGGG ACGGCGGCGG GGACGGCGGC GACGCGGGCC TGGCCAGCGC CTCGGCGCAG 
GGGCAGGCGG AGACCGTGCG GCAGCTCCTG GAAGCCGGCG CGGATCCCAA CGGAGTCAAC 
AGCTTCGGCA GGCGCCCGAT CCAGGTCATG ATGATGGGCA GCACCCGCGT GGCCCAGCTG 
CTGCTGCTCC ACGGCGCCGA CCCCAACTGT GCCGACCCCG CCACCCTCAC CCGCCCTGTG 
CACGACGCGG CCCGGGAGGG CTTCCTGGAC ACGCTGGTGG TGCTGCACCG AGCCGGGGCG 
CGGCTGGACG TGCGCGATGC CTGGGGCCGC CTGCCCGTGG ACCTGGCTGA GGAGCGGGGC 
CACGGCGCTG TCGCCCGGTA CCTGCGCGCA GCCGCGGGGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626467.1
CDS	454..918
Translation	MREEATGLLGGGGGGDGGGDGGGDGGGDGGDAGLASASAQGQAETVRQLLEAGADPNGVNSFGRRPIQVMMMGSTRVAQLLLLHGADPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEERGHGAVARYLRAAAGD

Target ORF information:

RefSeq Version	XM_005626410.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626410.2

ORF Insert Sequence:

ATGCGCGAGG AGGCCACGGG CTTGCTCGGC GGCGGCGGCG GCGGGGACGG CGGCGGGGAC 
GGCGGCGGGG ACGGCGGCGG GGACGGCGGC GACGCGGGCC TGGCCAGCGC CTCGGCGCAG 
GGGCAGGCGG AGACCGTGCG GCAGCTCCTG GAAGCCGGCG CGGATCCCAA CGGAGTCAAC 
AGCTTCGGCA GGCGCCCGAT CCAGGTCATG ATGATGGGCA GCACCCGCGT GGCCCAGCTG 
CTGCTGCTCC ACGGCGCCGA CCCCAACTGT GCCGACCCCG CCACCCTCAC CCGCCCTGTG 
CACGACGCGG CCCGGGAGGG CTTCCTGGAC ACGCTGGTGG TGCTGCACCG AGCCGGGGCG 
CGGCTGGACG TGCGCGATGC CTGGGGCCGC CTGCCCGTGG ACCTGGCTGA GGAGCGGGGC 
CACGGCGCTG TCGCCCGGTA CCTGCGCGCA GCCGCGGGGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDKN2B ( XM_005626410.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626467.1 Canis lupus familiaris cyclin dependent kinase inhibitor 2B (CDKN2B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe14144
Clone ID Related Accession (Same CDS sequence)	XM_005626410.2 , XM_005626410.3
Accession Version	XM_005626410.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cyclin-dependent kinase 4 inhibitor B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626410.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGAGG AGGCCACGGG CTTGCTCGGC GGCGGCGGCG GCGGGGACGG CGGCGGGGAC 
GGCGGCGGGG ACGGCGGCGG GGACGGCGGC GACGCGGGCC TGGCCAGCGC CTCGGCGCAG 
GGGCAGGCGG AGACCGTGCG GCAGCTCCTG GAAGCCGGCG CGGATCCCAA CGGAGTCAAC 
AGCTTCGGCA GGCGCCCGAT CCAGGTCATG ATGATGGGCA GCACCCGCGT GGCCCAGCTG 
CTGCTGCTCC ACGGCGCCGA CCCCAACTGT GCCGACCCCG CCACCCTCAC CCGCCCTGTG 
CACGACGCGG CCCGGGAGGG CTTCCTGGAC ACGCTGGTGG TGCTGCACCG AGCCGGGGCG 
CGGCTGGACG TGCGCGATGC CTGGGGCCGC CTGCCCGTGG ACCTGGCTGA GGAGCGGGGC 
CACGGCGCTG TCGCCCGGTA CCTGCGCGCA GCCGCGGGGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626467.1
CDS	454..918
Translation	MREEATGLLGGGGGGDGGGDGGGDGGGDGGDAGLASASAQGQAETVRQLLEAGADPNGVNSFGRRPIQVMMMGSTRVAQLLLLHGADPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEERGHGAVARYLRAAAGD

Target ORF information:

RefSeq Version	XM_005626410.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin dependent kinase inhibitor 2B (CDKN2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626410.3

ORF Insert Sequence:

ATGCGCGAGG AGGCCACGGG CTTGCTCGGC GGCGGCGGCG GCGGGGACGG CGGCGGGGAC 
GGCGGCGGGG ACGGCGGCGG GGACGGCGGC GACGCGGGCC TGGCCAGCGC CTCGGCGCAG 
GGGCAGGCGG AGACCGTGCG GCAGCTCCTG GAAGCCGGCG CGGATCCCAA CGGAGTCAAC 
AGCTTCGGCA GGCGCCCGAT CCAGGTCATG ATGATGGGCA GCACCCGCGT GGCCCAGCTG 
CTGCTGCTCC ACGGCGCCGA CCCCAACTGT GCCGACCCCG CCACCCTCAC CCGCCCTGTG 
CACGACGCGG CCCGGGAGGG CTTCCTGGAC ACGCTGGTGG TGCTGCACCG AGCCGGGGCG 
CGGCTGGACG TGCGCGATGC CTGGGGCCGC CTGCCCGTGG ACCTGGCTGA GGAGCGGGGC 
CACGGCGCTG TCGCCCGGTA CCTGCGCGCA GCCGCGGGGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide analyses implicate 33 loci in heritable dog osteosarcoma, including regulatory variants near CDKN2A/B.
Genome biology14(12)R132(2013 Dec)
Karlsson EK,Sigurdsson S,Ivansson E,Thomas R,Elvers I,Wright J,Howald C,Tonomura N,Perloski M,Swofford R,Biagi T,Fryc S,Anderson N,Courtay-Cahen C,Youell L,Ricketts SL,Mandlebaum S,Rivera P,von Euler H,Kisseberth WC,London CA,Lander ES,Couto G,Comstock K,Starkey MP,Modiano JF,Breen M,Lindblad-Toh K

Simultaneous inactivation of the p16, p15 and p14 genes encoding cyclin-dependent kinase inhibitors in canine T-lymphoid tumor cells.
The Journal of veterinary medical science75(6)733-42(2013)
Fujiwara-Igarashi A,Goto-Koshino Y,Mochizuki H,Maeda S,Fujino Y,Ohno K,Tsujimoto H

Disruption of chromosome 11 in canine fibrosarcomas highlights an unusual variability of CDKN2B in dogs.
BMC veterinary research527(2009 Jul)
Aguirre-Hern?ndez J,Milne BS,Queen C,O'Brien PC,Hoather T,Haugland S,Ferguson-Smith MA,Dobson JM,Sargan DR

Inactivation of the p16 cyclin-dependent kinase inhibitor in high-grade canine non-Hodgkin's T-cell lymphoma.
Veterinary pathology44(4)467-78(2007 Jul)
Fosmire SP,Thomas R,Jubala CM,Wojcieszyn JW,Valli VE,Getzy DM,Smith TL,Gardner LA,Ritt MG,Bell JS,Freeman KP,Greenfield BE,Lana SE,Kisseberth WC,Helfand SC,Cutter GR,Breen M,Modiano JF

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CDKN2B cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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