VIL1 cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	VIL1
Entrez Gene ID	103110705
Full Name	villin 1
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016187044.1	XP_016042530.1	villin-1 isoform X2
XM_007519877.2	XP_007519939.1	villin-1 isoform X1

Pathways from BioSystems
Gene Ontology

The following VIL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VIL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEr00828	XM_007519877.2 Latest version!	Erinaceus europaeus villin 1 (VIL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OEr00829	XM_016187044.1 Latest version!	Erinaceus europaeus villin 1 (VIL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VIL1 ( XM_007519877.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007519939.1 Erinaceus europaeus villin 1 (VIL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OEr00828
Clone ID Related Accession (Same CDS sequence)	XM_007519877.2
Accession Version	XM_007519877.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	villin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006803931.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007519877.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAAGC TCAGCACCCA AGTCAAAGGC TCCCTCAACA CCACCAGTCC CGGGGTGCAG 
ATATGGAGGA TCGAGGCCAT GCAGATGGTG CCTGTCCCCT CCAGCACCTT CGGCAGCTTC 
TTTGATGGCG ACTGCTACAT CGTCTTGGCT ATCCACAAGA CAGGCAGCAC CTTGTCCTAT 
GACATCCACT TCTGGATCGG CCAGGCCTCA TCCCAGGATG AGCAGGGGGC AGCCGCCATC 
TACACCACAC AGATGGATGA CTTCCTCAAG GGCAAGGCTG TGCAACACCG TGAGGTCCAA 
GGCAATGAGA GCGACACCTT CCGCGCCTAC TTCAAGCAGG GTCTCGTGAT CCGGAAAGGG 
GGTGTGGCGT CTGGGATGAA GCACGTGGAG ACCAACTCCT ATGACGTCCA GCGGCTGCTG 
CACGTCAAGG GCAAGAGGAA TGTGGTGGCC GGAGAGGTGG AGAAGTCCTG GAAGAGTTTC 
AACCGCGGGG ACGTTTTCCT CCTGGACCTG GGGAAGCTCA TCATCCAGTG GAACGGGCCC 
GAGAGCAACC GCATGGAGAG ACTCAGGGGC ATGAACCTGG CTAAGGAGAT CCGTGACCAG 
GAACGAGGCG GGCGCACATA CGTGGCTGTG GTGGATGGCG AGAGCGAGAA GGACTCACCG 
CAGCTGATGG AGATCATGAA CTACGTGCTG GGCCAGCGGC GGGAGCTCAA GGCCGCCATC 
CCAGACAACT TGGTAGAGCC AGCACTCAAG GCCAACCTCA AGCTATACCA CTTGTCTGAC 
TCAGAGGGAA AGCTGGTTGT CAGAGAGATC ACCACTCGGC CACTCACCCA AAACCTGCTC 
AACCACGAGG ACTGCTACAT CCTGGACCAG GGCGGGTTGA AGATCTTCGT GTGGAAGGGC 
AAGAAAGCCA ATGCCCAGGA GAAGAAGGAG GCCATGAACC AGGCGCTGAA CTTCATCAAA 
GCGAAGCAGT ACCCGCAGAG CACACAGGTG GAGGTGCAGA ATGACGGGGC CGAGTCCGCG 
GTCTTCCAAC AGCTTTTCCA GAAGTGGACA GTGCCCAACC GCACCTCGGG CCTGGGCACG 
ACCCACAGCG TGGGTTCTGT GGCCAAGGTG GAGCAGGTGA AGTTTGATGC CATGTCCATG 
CATGTCCAGC CCCAGGTGGC CGCCCAGCAG AAGATGGTGG ATGATGGCAG CGGGGACGTG 
CAGATTTGGC GCATCGAGGA CCTGGAGATG GTGCCCGTGG ACCCCAAGTG GCGGGGCCAC 
TTCTACGGGG GTGACTGCTA CCTGCTGCTC TACACCTACC TCATCGGGGA GAAGCAGCAC 
TACCTGCTCT ACATCTGGCA GGGCGCCCAG GCCAGCCAGG ATGAGATCGC AGCTTCGGCC 
TACCAGGCTG TCAACTTGGA CCAGCAGTAC AACGACGAGG CCGTCCAGAT CCGGGTCCCG 
ATGGGCAAGG AGCCACCGCA CCTCATGTCC ATCTTCAAGG GCCGCATGGT GGTCTACCAG 
GGAGGCACCT CCCGGGCTAA CAGTACTGAG CCCATCCCCT CCACAAGGCT GTTCCAGGTC 
CGGGGGACCA ATGACAACAA CACGAAGGCT TTCGAGGTCC CAGCCCGAGC TGCTTCCCTC 
AATTCCAACG ATGTCTTCGT CCTGAAGACC CAGTCTTGCT GCTTCTTGTG GTGCGGGAAG 
GGCTGCAGCG GTGATGAGCG GGAGATGGCC AAGCTCGTGG CTGACACCAT CTCCCGGACG 
GAGAAGCAAG TGGTGGTGGA AGGACAGGAG CCCAGCAGCT TCTGGGTGGC CCTAGGCGGG 
AAGGCGCCCT ACTCCAACAG CAAGAGACTG CAGGAGGAGA CCCAGGTCAT CACACCCCGG 
CTCTTTGAAT GCTCCAACCA GACGGGGCGA TTCCTGGCCA CAGAGATCCC TGACTTCAGC 
CAGGATGACT TGGAGGAGGA CGACGTGTTC CTCCTGGACG TCTGGGATCA GGTCTTCTTC 
TGGATTGGGA AGCAAGCCAA CGAGGAGGAG AAGAAAGCCA CAGCTGTGAC GGTGCAAGAG 
TACCTCAAGA CCCACCCCAG TGGCCGAGAC CCTCAGACGC CCATCATTGT GGTCAAGCAG 
GGCCACGAGC CCCCCACTTT CACTGGCTGG TTCCTGGCTT GGGACCCCTT CAAGTGGAGT 
AACACCAAGT CCTATGAGGA CGTGAAGGGG GAGCTTGGAA ACCCTGGAGA CTGGGATCAC 
ATCACCGCTG AGATTACCAA TCCTAAAACA GACGTGTTCA ACGCCAACAG CAGCCTCGAT 
TCTGGGCCTC TCCCCATCTT CCCACTGGAG CAGTTGATCA ATGTTCCTGT GGACGAGCTC 
CCTGAGGGTG TGGACCCCAG CAGGAAGGAG GAGCACCTGT CTGTTGAGGA TTTCACCAAG 
GCCCTGGGGA TGACTCCAGC TGCTTTCTCT GCCCTGCCTC GTTGGAAGCA ACAGAACCTC 
AAGAAACAGA AAGGCCTCTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007519939.1
CDS	50..2533
Translation	MTKLSTQVKGSLNTTSPGVQIWRIEAMQMVPVPSSTFGSFFDGDCYIVLAIHKTGSTLSYDIHFWIGQASSQDEQGAAAIYTTQMDDFLKGKAVQHREVQGNESDTFRAYFKQGLVIRKGGVASGMKHVETNSYDVQRLLHVKGKRNVVAGEVEKSWKSFNRGDVFLLDLGKLIIQWNGPESNRMERLRGMNLAKEIRDQERGGRTYVAVVDGESEKDSPQLMEIMNYVLGQRRELKAAIPDNLVEPALKANLKLYHLSDSEGKLVVREITTRPLTQNLLNHEDCYILDQGGLKIFVWKGKKANAQEKKEAMNQALNFIKAKQYPQSTQVEVQNDGAESAVFQQLFQKWTVPNRTSGLGTTHSVGSVAKVEQVKFDAMSMHVQPQVAAQQKMVDDGSGDVQIWRIEDLEMVPVDPKWRGHFYGGDCYLLLYTYLIGEKQHYLLYIWQGAQASQDEIAASAYQAVNLDQQYNDEAVQIRVPMGKEPPHLMSIFKGRMVVYQGGTSRANSTEPIPSTRLFQVRGTNDNNTKAFEVPARAASLNSNDVFVLKTQSCCFLWCGKGCSGDEREMAKLVADTISRTEKQVVVEGQEPSSFWVALGGKAPYSNSKRLQEETQVITPRLFECSNQTGRFLATEIPDFSQDDLEEDDVFLLDVWDQVFFWIGKQANEEEKKATAVTVQEYLKTHPSGRDPQTPIIVVKQGHEPPTFTGWFLAWDPFKWSNTKSYEDVKGELGNPGDWDHITAEITNPKTDVFNANSSLDSGPLPIFPLEQLINVPVDELPEGVDPSRKEEHLSVEDFTKALGMTPAAFSALPRWKQQNLKKQKGLF

Target ORF information:

RefSeq Version	XM_007519877.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus villin 1 (VIL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007519877.2

ORF Insert Sequence:

ATGACCAAGC TCAGCACCCA AGTCAAAGGC TCCCTCAACA CCACCAGTCC CGGGGTGCAG 
ATATGGAGGA TCGAGGCCAT GCAGATGGTG CCTGTCCCCT CCAGCACCTT CGGCAGCTTC 
TTTGATGGCG ACTGCTACAT CGTCTTGGCT ATCCACAAGA CAGGCAGCAC CTTGTCCTAT 
GACATCCACT TCTGGATCGG CCAGGCCTCA TCCCAGGATG AGCAGGGGGC AGCCGCCATC 
TACACCACAC AGATGGATGA CTTCCTCAAG GGCAAGGCTG TGCAACACCG TGAGGTCCAA 
GGCAATGAGA GCGACACCTT CCGCGCCTAC TTCAAGCAGG GTCTCGTGAT CCGGAAAGGG 
GGTGTGGCGT CTGGGATGAA GCACGTGGAG ACCAACTCCT ATGACGTCCA GCGGCTGCTG 
CACGTCAAGG GCAAGAGGAA TGTGGTGGCC GGAGAGGTGG AGAAGTCCTG GAAGAGTTTC 
AACCGCGGGG ACGTTTTCCT CCTGGACCTG GGGAAGCTCA TCATCCAGTG GAACGGGCCC 
GAGAGCAACC GCATGGAGAG ACTCAGGGGC ATGAACCTGG CTAAGGAGAT CCGTGACCAG 
GAACGAGGCG GGCGCACATA CGTGGCTGTG GTGGATGGCG AGAGCGAGAA GGACTCACCG 
CAGCTGATGG AGATCATGAA CTACGTGCTG GGCCAGCGGC GGGAGCTCAA GGCCGCCATC 
CCAGACAACT TGGTAGAGCC AGCACTCAAG GCCAACCTCA AGCTATACCA CTTGTCTGAC 
TCAGAGGGAA AGCTGGTTGT CAGAGAGATC ACCACTCGGC CACTCACCCA AAACCTGCTC 
AACCACGAGG ACTGCTACAT CCTGGACCAG GGCGGGTTGA AGATCTTCGT GTGGAAGGGC 
AAGAAAGCCA ATGCCCAGGA GAAGAAGGAG GCCATGAACC AGGCGCTGAA CTTCATCAAA 
GCGAAGCAGT ACCCGCAGAG CACACAGGTG GAGGTGCAGA ATGACGGGGC CGAGTCCGCG 
GTCTTCCAAC AGCTTTTCCA GAAGTGGACA GTGCCCAACC GCACCTCGGG CCTGGGCACG 
ACCCACAGCG TGGGTTCTGT GGCCAAGGTG GAGCAGGTGA AGTTTGATGC CATGTCCATG 
CATGTCCAGC CCCAGGTGGC CGCCCAGCAG AAGATGGTGG ATGATGGCAG CGGGGACGTG 
CAGATTTGGC GCATCGAGGA CCTGGAGATG GTGCCCGTGG ACCCCAAGTG GCGGGGCCAC 
TTCTACGGGG GTGACTGCTA CCTGCTGCTC TACACCTACC TCATCGGGGA GAAGCAGCAC 
TACCTGCTCT ACATCTGGCA GGGCGCCCAG GCCAGCCAGG ATGAGATCGC AGCTTCGGCC 
TACCAGGCTG TCAACTTGGA CCAGCAGTAC AACGACGAGG CCGTCCAGAT CCGGGTCCCG 
ATGGGCAAGG AGCCACCGCA CCTCATGTCC ATCTTCAAGG GCCGCATGGT GGTCTACCAG 
GGAGGCACCT CCCGGGCTAA CAGTACTGAG CCCATCCCCT CCACAAGGCT GTTCCAGGTC 
CGGGGGACCA ATGACAACAA CACGAAGGCT TTCGAGGTCC CAGCCCGAGC TGCTTCCCTC 
AATTCCAACG ATGTCTTCGT CCTGAAGACC CAGTCTTGCT GCTTCTTGTG GTGCGGGAAG 
GGCTGCAGCG GTGATGAGCG GGAGATGGCC AAGCTCGTGG CTGACACCAT CTCCCGGACG 
GAGAAGCAAG TGGTGGTGGA AGGACAGGAG CCCAGCAGCT TCTGGGTGGC CCTAGGCGGG 
AAGGCGCCCT ACTCCAACAG CAAGAGACTG CAGGAGGAGA CCCAGGTCAT CACACCCCGG 
CTCTTTGAAT GCTCCAACCA GACGGGGCGA TTCCTGGCCA CAGAGATCCC TGACTTCAGC 
CAGGATGACT TGGAGGAGGA CGACGTGTTC CTCCTGGACG TCTGGGATCA GGTCTTCTTC 
TGGATTGGGA AGCAAGCCAA CGAGGAGGAG AAGAAAGCCA CAGCTGTGAC GGTGCAAGAG 
TACCTCAAGA CCCACCCCAG TGGCCGAGAC CCTCAGACGC CCATCATTGT GGTCAAGCAG 
GGCCACGAGC CCCCCACTTT CACTGGCTGG TTCCTGGCTT GGGACCCCTT CAAGTGGAGT 
AACACCAAGT CCTATGAGGA CGTGAAGGGG GAGCTTGGAA ACCCTGGAGA CTGGGATCAC 
ATCACCGCTG AGATTACCAA TCCTAAAACA GACGTGTTCA ACGCCAACAG CAGCCTCGAT 
TCTGGGCCTC TCCCCATCTT CCCACTGGAG CAGTTGATCA ATGTTCCTGT GGACGAGCTC 
CCTGAGGGTG TGGACCCCAG CAGGAAGGAG GAGCACCTGT CTGTTGAGGA TTTCACCAAG 
GCCCTGGGGA TGACTCCAGC TGCTTTCTCT GCCCTGCCTC GTTGGAAGCA ACAGAACCTC 
AAGAAACAGA AAGGCCTCTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VIL1 ( XM_016187044.1 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_016042530.1 Erinaceus europaeus villin 1 (VIL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OEr00829
Clone ID Related Accession (Same CDS sequence)	XM_016187044.1
Accession Version	XM_016187044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	villin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006803931.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAAGC TCAGCACCCA AGTCAAAGGC TCCCTCAACA CCACCAGTCC CGGGGTGCAG 
ATATGGAGGA TCGAGATCCA CAAGACAGGC AGCACCTTGT CCTATGACAT CCACTTCTGG 
ATCGGCCAGG CCTCATCCCA GGATGAGCAG GGGGCAGCCG CCATCTACAC CACACAGATG 
GATGACTTCC TCAAGGGCAA GGCTGTGCAA CACCGTGAGG TCCAAGGCAA TGAGAGCGAC 
ACCTTCCGCG CCTACTTCAA GCAGGGTCTC GTGATCCGGA AAGGGGGTGT GGCGTCTGGG 
ATGAAGCACG TGGAGACCAA CTCCTATGAC GTCCAGCGGC TGCTGCACGT CAAGGGCAAG 
AGGAATGTGG TGGCCGGAGA GGTGGAGAAG TCCTGGAAGA GTTTCAACCG CGGGGACGTT 
TTCCTCCTGG ACCTGGGGAA GCTCATCATC CAGTGGAACG GGCCCGAGAG CAACCGCATG 
GAGAGACTCA GGGGCATGAA CCTGGCTAAG GAGATCCGTG ACCAGGAACG AGGCGGGCGC 
ACATACGTGG CTGTGGTGGA TGGCGAGAGC GAGAAGGACT CACCGCAGCT GATGGAGATC 
ATGAACTACG TGCTGGGCCA GCGGCGGGAG CTCAAGGCCG CCATCCCAGA CAACTTGGTA 
GAGCCAGCAC TCAAGGCCAA CCTCAAGCTA TACCACTTGT CTGACTCAGA GGGAAAGCTG 
GTTGTCAGAG AGATCACCAC TCGGCCACTC ACCCAAAACC TGCTCAACCA CGAGGACTGC 
TACATCCTGG ACCAGGGCGG GTTGAAGATC TTCGTGTGGA AGGGCAAGAA AGCCAATGCC 
CAGGAGAAGA AGGAGGCCAT GAACCAGGCG CTGAACTTCA TCAAAGCGAA GCAGTACCCG 
CAGAGCACAC AGGTGGAGGT GCAGAATGAC GGGGCCGAGT CCGCGGTCTT CCAACAGCTT 
TTCCAGAAGT GGACAGTGCC CAACCGCACC TCGGGCCTGG GCACGACCCA CAGCGTGGGT 
TCTGTGGCCA AGGTGGAGCA GGTGAAGTTT GATGCCATGT CCATGCATGT CCAGCCCCAG 
GTGGCCGCCC AGCAGAAGAT GGTGGATGAT GGCAGCGGGG ACGTGCAGAT TTGGCGCATC 
GAGGACCTGG AGATGGTGCC CGTGGACCCC AAGTGGCGGG GCCACTTCTA CGGGGGTGAC 
TGCTACCTGC TGCTCTACAC CTACCTCATC GGGGAGAAGC AGCACTACCT GCTCTACATC 
TGGCAGGGCG CCCAGGCCAG CCAGGATGAG ATCGCAGCTT CGGCCTACCA GGCTGTCAAC 
TTGGACCAGC AGTACAACGA CGAGGCCGTC CAGATCCGGG TCCCGATGGG CAAGGAGCCA 
CCGCACCTCA TGTCCATCTT CAAGGGCCGC ATGGTGGTCT ACCAGGGAGG CACCTCCCGG 
GCTAACAGTA CTGAGCCCAT CCCCTCCACA AGGCTGTTCC AGGTCCGGGG GACCAATGAC 
AACAACACGA AGGCTTTCGA GGTCCCAGCC CGAGCTGCTT CCCTCAATTC CAACGATGTC 
TTCGTCCTGA AGACCCAGTC TTGCTGCTTC TTGTGGTGCG GGAAGGGCTG CAGCGGTGAT 
GAGCGGGAGA TGGCCAAGCT CGTGGCTGAC ACCATCTCCC GGACGGAGAA GCAAGTGGTG 
GTGGAAGGAC AGGAGCCCAG CAGCTTCTGG GTGGCCCTAG GCGGGAAGGC GCCCTACTCC 
AACAGCAAGA GACTGCAGGA GGAGACCCAG GTCATCACAC CCCGGCTCTT TGAATGCTCC 
AACCAGACGG GGCGATTCCT GGCCACAGAG ATCCCTGACT TCAGCCAGGA TGACTTGGAG 
GAGGACGACG TGTTCCTCCT GGACGTCTGG GATCAGGTCT TCTTCTGGAT TGGGAAGCAA 
GCCAACGAGG AGGAGAAGAA AGCCACAGCT GTGACGGTGC AAGAGTACCT CAAGACCCAC 
CCCAGTGGCC GAGACCCTCA GACGCCCATC ATTGTGGTCA AGCAGGGCCA CGAGCCCCCC 
ACTTTCACTG GCTGGTTCCT GGCTTGGGAC CCCTTCAAGT GGAGTAACAC CAAGTCCTAT 
GAGGACGTGA AGGGGGAGCT TGGAAACCCT GGAGACTGGG ATCACATCAC CGCTGAGATT 
ACCAATCCTA AAACAGACGT GTTCAACGCC AACAGCAGCC TCGATTCTGG GCCTCTCCCC 
ATCTTCCCAC TGGAGCAGTT GATCAATGTT CCTGTGGACG AGCTCCCTGA GGGTGTGGAC 
CCCAGCAGGA AGGAGGAGCA CCTGTCTGTT GAGGATTTCA CCAAGGCCCT GGGGATGACT 
CCAGCTGCTT TCTCTGCCCT GCCTCGTTGG AAGCAACAGA ACCTCAAGAA ACAGAAAGGC 
CTCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016042530.1
CDS	50..2458
Translation	MTKLSTQVKGSLNTTSPGVQIWRIEIHKTGSTLSYDIHFWIGQASSQDEQGAAAIYTTQMDDFLKGKAVQHREVQGNESDTFRAYFKQGLVIRKGGVASGMKHVETNSYDVQRLLHVKGKRNVVAGEVEKSWKSFNRGDVFLLDLGKLIIQWNGPESNRMERLRGMNLAKEIRDQERGGRTYVAVVDGESEKDSPQLMEIMNYVLGQRRELKAAIPDNLVEPALKANLKLYHLSDSEGKLVVREITTRPLTQNLLNHEDCYILDQGGLKIFVWKGKKANAQEKKEAMNQALNFIKAKQYPQSTQVEVQNDGAESAVFQQLFQKWTVPNRTSGLGTTHSVGSVAKVEQVKFDAMSMHVQPQVAAQQKMVDDGSGDVQIWRIEDLEMVPVDPKWRGHFYGGDCYLLLYTYLIGEKQHYLLYIWQGAQASQDEIAASAYQAVNLDQQYNDEAVQIRVPMGKEPPHLMSIFKGRMVVYQGGTSRANSTEPIPSTRLFQVRGTNDNNTKAFEVPARAASLNSNDVFVLKTQSCCFLWCGKGCSGDEREMAKLVADTISRTEKQVVVEGQEPSSFWVALGGKAPYSNSKRLQEETQVITPRLFECSNQTGRFLATEIPDFSQDDLEEDDVFLLDVWDQVFFWIGKQANEEEKKATAVTVQEYLKTHPSGRDPQTPIIVVKQGHEPPTFTGWFLAWDPFKWSNTKSYEDVKGELGNPGDWDHITAEITNPKTDVFNANSSLDSGPLPIFPLEQLINVPVDELPEGVDPSRKEEHLSVEDFTKALGMTPAAFSALPRWKQQNLKKQKGLF

Target ORF information:

RefSeq Version	XM_016187044.1
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus villin 1 (VIL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016187044.1

ORF Insert Sequence:

ATGACCAAGC TCAGCACCCA AGTCAAAGGC TCCCTCAACA CCACCAGTCC CGGGGTGCAG 
ATATGGAGGA TCGAGATCCA CAAGACAGGC AGCACCTTGT CCTATGACAT CCACTTCTGG 
ATCGGCCAGG CCTCATCCCA GGATGAGCAG GGGGCAGCCG CCATCTACAC CACACAGATG 
GATGACTTCC TCAAGGGCAA GGCTGTGCAA CACCGTGAGG TCCAAGGCAA TGAGAGCGAC 
ACCTTCCGCG CCTACTTCAA GCAGGGTCTC GTGATCCGGA AAGGGGGTGT GGCGTCTGGG 
ATGAAGCACG TGGAGACCAA CTCCTATGAC GTCCAGCGGC TGCTGCACGT CAAGGGCAAG 
AGGAATGTGG TGGCCGGAGA GGTGGAGAAG TCCTGGAAGA GTTTCAACCG CGGGGACGTT 
TTCCTCCTGG ACCTGGGGAA GCTCATCATC CAGTGGAACG GGCCCGAGAG CAACCGCATG 
GAGAGACTCA GGGGCATGAA CCTGGCTAAG GAGATCCGTG ACCAGGAACG AGGCGGGCGC 
ACATACGTGG CTGTGGTGGA TGGCGAGAGC GAGAAGGACT CACCGCAGCT GATGGAGATC 
ATGAACTACG TGCTGGGCCA GCGGCGGGAG CTCAAGGCCG CCATCCCAGA CAACTTGGTA 
GAGCCAGCAC TCAAGGCCAA CCTCAAGCTA TACCACTTGT CTGACTCAGA GGGAAAGCTG 
GTTGTCAGAG AGATCACCAC TCGGCCACTC ACCCAAAACC TGCTCAACCA CGAGGACTGC 
TACATCCTGG ACCAGGGCGG GTTGAAGATC TTCGTGTGGA AGGGCAAGAA AGCCAATGCC 
CAGGAGAAGA AGGAGGCCAT GAACCAGGCG CTGAACTTCA TCAAAGCGAA GCAGTACCCG 
CAGAGCACAC AGGTGGAGGT GCAGAATGAC GGGGCCGAGT CCGCGGTCTT CCAACAGCTT 
TTCCAGAAGT GGACAGTGCC CAACCGCACC TCGGGCCTGG GCACGACCCA CAGCGTGGGT 
TCTGTGGCCA AGGTGGAGCA GGTGAAGTTT GATGCCATGT CCATGCATGT CCAGCCCCAG 
GTGGCCGCCC AGCAGAAGAT GGTGGATGAT GGCAGCGGGG ACGTGCAGAT TTGGCGCATC 
GAGGACCTGG AGATGGTGCC CGTGGACCCC AAGTGGCGGG GCCACTTCTA CGGGGGTGAC 
TGCTACCTGC TGCTCTACAC CTACCTCATC GGGGAGAAGC AGCACTACCT GCTCTACATC 
TGGCAGGGCG CCCAGGCCAG CCAGGATGAG ATCGCAGCTT CGGCCTACCA GGCTGTCAAC 
TTGGACCAGC AGTACAACGA CGAGGCCGTC CAGATCCGGG TCCCGATGGG CAAGGAGCCA 
CCGCACCTCA TGTCCATCTT CAAGGGCCGC ATGGTGGTCT ACCAGGGAGG CACCTCCCGG 
GCTAACAGTA CTGAGCCCAT CCCCTCCACA AGGCTGTTCC AGGTCCGGGG GACCAATGAC 
AACAACACGA AGGCTTTCGA GGTCCCAGCC CGAGCTGCTT CCCTCAATTC CAACGATGTC 
TTCGTCCTGA AGACCCAGTC TTGCTGCTTC TTGTGGTGCG GGAAGGGCTG CAGCGGTGAT 
GAGCGGGAGA TGGCCAAGCT CGTGGCTGAC ACCATCTCCC GGACGGAGAA GCAAGTGGTG 
GTGGAAGGAC AGGAGCCCAG CAGCTTCTGG GTGGCCCTAG GCGGGAAGGC GCCCTACTCC 
AACAGCAAGA GACTGCAGGA GGAGACCCAG GTCATCACAC CCCGGCTCTT TGAATGCTCC 
AACCAGACGG GGCGATTCCT GGCCACAGAG ATCCCTGACT TCAGCCAGGA TGACTTGGAG 
GAGGACGACG TGTTCCTCCT GGACGTCTGG GATCAGGTCT TCTTCTGGAT TGGGAAGCAA 
GCCAACGAGG AGGAGAAGAA AGCCACAGCT GTGACGGTGC AAGAGTACCT CAAGACCCAC 
CCCAGTGGCC GAGACCCTCA GACGCCCATC ATTGTGGTCA AGCAGGGCCA CGAGCCCCCC 
ACTTTCACTG GCTGGTTCCT GGCTTGGGAC CCCTTCAAGT GGAGTAACAC CAAGTCCTAT 
GAGGACGTGA AGGGGGAGCT TGGAAACCCT GGAGACTGGG ATCACATCAC CGCTGAGATT 
ACCAATCCTA AAACAGACGT GTTCAACGCC AACAGCAGCC TCGATTCTGG GCCTCTCCCC 
ATCTTCCCAC TGGAGCAGTT GATCAATGTT CCTGTGGACG AGCTCCCTGA GGGTGTGGAC 
CCCAGCAGGA AGGAGGAGCA CCTGTCTGTT GAGGATTTCA CCAAGGCCCT GGGGATGACT 
CCAGCTGCTT TCTCTGCCCT GCCTCGTTGG AAGCAACAGA ACCTCAAGAA ACAGAAAGGC 
CTCTTTTGA